LOCUS       DASWNU010000001         5343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_259483, whole genome shotgun sequence.
ACCESSION   DASWNU010000001 DASWNU010000000
VERSION     DASWNU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259483"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGB57_00005"
     CDS             complement(<1..648)
                     /locus_tag="VGB57_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0129757.1"
                     /translation="MARFEFKLPDIGEGIAEAEIVAWHIKIGDSVREDQQLADMMTDK
                     ATVEMESPVAGKVLELAGEVGDQIPIGSVLVVFETEAEASVAEQDGQETTEEGERTDT
                     VASADGLITPTEAQKEAIPSTSVEETPLASSEVEKHAPEQRVSTLLDTNGVGERKSVL
                     ASPAVRQRARDLGIDLAQVKTAGDRIRHADLDSYLLYSGGSVSRPGAARRQDKTIK"
     gene            complement(777..1781)
                     /locus_tag="VGB57_00010"
                     /pseudo
     CDS             complement(777..1781)
                     /locus_tag="VGB57_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
     gene            complement(1787..3058)
                     /locus_tag="VGB57_00015"
     CDS             complement(1787..3058)
                     /locus_tag="VGB57_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEY0129758.1"
                     /translation="MNTASERPNRPALSLYVPEPPARPGDEVDFSHIEIPAAGSVPRP
                     DSAAPAADTHALVYSLVRVLDDEGNAVGPWDPKLDPDTLRRILRDMMMVRTYDDRMYR
                     AQRQGKTSFYMKSTGEEAIAVAGAHALDRDDMCFPTYRQQGLLVARDYPLVQMMCQVY
                     SNRGDPLKGRQLPIMYASNSHGFFTISGNLATQFPQAVGWAMASAIRGDSRIASGYIG
                     DGSTAEGDFHAACTFAGVYRAPVILNVVNNQWAISTFSGIAGGDLTTFAARAVGYGIA
                     GLRVDGNDALAVYAATLWAADRARANLGPTLIEFFTYRVEGHSTSDDPSVYRPQNAGA
                     AWPLGDPVARLRQHLVALGEWDDERHQAQLDQLDAEVRAAQKEAEKLGTLQSDPYLDI
                     ASMFEDVFEELPWHLKEQQAQAIAEGEAKRK"
     gene            3161..3655
                     /locus_tag="VGB57_00020"
     CDS             3161..3655
                     /locus_tag="VGB57_00020"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEY0129759.1"
                     /translation="MALFRPFAALLLLAASACATTAKDPPAPSWYVMRHLQKAEGQDP
                     ALSAEGHANARRLIDFFAADPPRAIYVSTTRRALETAGPLASRLGVTVKDYDPRDTAG
                     LVARVKAEAGTVLVVGHGNTVPEIVAQLGGARPGELTETDFGDIFRVRRDGKVERLRL
                     ETAP"
     gene            complement(3788..4018)
                     /locus_tag="VGB57_00025"
     CDS             complement(3788..4018)
                     /locus_tag="VGB57_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129760.1"
                     /translation="MSEPMTMMAMAGFGLTALGILLFAASKGWQGWLDLKRLELAGAE
                     PRGHGGMAERIELADLKERIRKLESLAACIDL"
     gene            complement(4045..4392)
                     /locus_tag="VGB57_00030"
     CDS             complement(4045..4392)
                     /locus_tag="VGB57_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY0129761.1"
                     /translation="MKKRFYLDKEEGKVFGVCAGIANHMNWDPTFVRVAMVVLTLITF
                     PWALLVYGVAAMIGKKRPVDGYGEAALDRPLRTSTYELNRSMRDIDRRMAEIETYVTA
                     QNTPLAREIESLR"
     gene            complement(4521..5195)
                     /gene="pspA"
                     /locus_tag="VGB57_00035"
     CDS             complement(4521..5195)
                     /gene="pspA"
                     /locus_tag="VGB57_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEY0129762.1"
                     /translation="MSIFSRTRDIVAANFSDLLDKAEDPAKMIRMIILEMEETLVEVR
                     ASTARTMADQKEMHRQIARLDRLQESWAEKAELALGKGREDLAHGALTEKRKAAELAE
                     SLRTEIDLLDEAMRASEADIARLQGKLREARIRQSATRTRLESANQRARLREMTAGPR
                     VDEAFSRFDRLEREVDHAEGRADALMLGAPPRSLDEEIAELRNAEKIDAELEALKARL
                     AADREG"
BASE COUNT          784 a         1690 c         1905 g          964 t
ORIGIN      
        1 cttgatggtt ttgtcctgac gccttgcggc gccaggccgg gagacggagc cgccgctgta
       61 gagtaggtag gagtcgaggt cggcgtggcg gattcggtcg ccggcggtct tcacctgggc
      121 aaggtcgatg ccgagatcac gggcgcgctg acgcaccgcg ggtgacgcga ggaccgattt
      181 ccgctcaccc actccgttcg tgtcgagcaa agtcgagaca cgctgctcag gcgcatgctt
      241 ctcgacttcg ctcgaagcga gcggtgtctc ttcgaccgag gtggagggga tcgcttcctt
      301 ctgggcctcg gtcggggtga tgaggccgtc ggcggaggcg acggtgtcgg tccgctcccc
      361 ttcctcggtc gtctcctggc cgtcctgctc ggcgacggaa gcctcggctt ccgtctcgaa
      421 caccaccagc accgagccga tcgggatctg gtcgccgacc tcgccggcaa gctcgagcac
      481 cttgccggcg acggggcttt ccatctcgac cgtggctttg tcggtcatca tgtcggcgag
      541 ctgctggtcc tcccggacgg agtcgccgat cttgatgtgc caggcgacga tctcggcctc
      601 ggcaatgcct tcgccgatgt cgggcagctt gaattcgaag cgcgccatgg gcgtcccttt
      661 agtccttggt cgtcattgcg aggagcggag cgacgaagca atccagcctg cgtccgacgc
      721 cgcgctggat tgcttcgcta cgctcgcaat gacgggatga tgcggccatc atcccgtcag
      781 tcctccaaag cttttttcag cgccagcccg agccggaccg ggccggggaa ataggcccat
      841 tccatcgagt gcggataagg cgtgtcccag ccggtgaccc gggtgacggg cgcctcgaga
      901 tggtagaagc agcgctcctg gacgagcgcc gacaattcgg cgccgaagcc cgaggtccgg
      961 gtcgcctcgt ggacgatcag gcagcgaccg gtcttcctca ccgaagcctc gatcgtctcg
     1021 atgtcgacgg gcacgagggt gcgaaggtcg atcacctcgg cgtcgactcc ttctgccgcg
     1081 gcggtggcga gggcgacgtg gaccatcgtg ccgtaggcga ggatggtgat ctcctcccct
     1141 tcgcgcacca ccgccgcctt gccgagcggg acggtatagt ggccgtccgg cacctcggcg
     1201 gcgggatgct tggcccaggg ggtgacgggg cgttcgtaga agccgtcgaa cgggccgttg
     1261 tagatccgct tgggctcgaa gaagatgacc gggtcgttgt cctcgatcgc ggcgatgagg
     1321 aggcccttgg cgtcgtaggg gttggagggg atcaccgtct tgattccggc gatgtgggtg
     1381 aacagggcct cgggcgactg gctgagggtc tggcctccga agatgccgcc gccctagggg
     1441 gagcgcagga ccatcggcag atgccattcg cccgccgagc ggtaacgcat cttggcggct
     1501 tcggagacga tctggtcgaa gcccggatag atgtagtcgg cgaactggat ctcggcgacg
     1561 gggcgaaggc cgtaggcggc cattccgacg gcgatcgcga cgatgccgtt ctcgctgatc
     1621 ggggagtcga aggcgcgctc cttgccgaat ttcttctgga ggtgctcggt gacgcggaag
     1681 accccgccga aatagccgat gtcctcgccg aggacgacca tggccggatc ccgctccatc
     1741 atcacgtgat gggccgagtt gagcgcctgg atcatgttca tgacgctcat ttgcgcttcg
     1801 cctcgccttc cgcgatcgcc tgggcctgct gttccttgag atgccaggga agctcctcga
     1861 acacgtcctc gaacatcgag gcgatgtcga gatagggatc ggactgcagg gtaccgagct
     1921 tctcggcctc cttctgggcg gcgcggacct cggcgtcgag ctggtcgagc tgggcctgat
     1981 ggcgttcgtc gtcccattcg ccgagggcga cgaggtgctg cctcaggcgg gcgacgggat
     2041 cgcccagcgg ccacgcggcc ccggcattct ggggccggta gacggaggga tcgtccgagg
     2101 tggaatggcc ctcgacccgg taggtgaaga attcgatcag ggtgggcccg agattggcgc
     2161 gggcgcggtc cgcggcccat aaagtcgcgg cgtagacggc cagggcgtcg ttgccgtcga
     2221 ccctcaggcc ggcgatgccg tagccgacgg cgcgggcggc gaaggtggtg agatcgccgc
     2281 cggcgatccc ggagaaggtc gagatcgccc actggttgtt gacgacgttg aggatgaccg
     2341 gcgcccggta gactccggcg aaggtgcagg cggcgtggaa atcgccctcg gcggtggagc
     2401 cgtcgccgat atagcccgag gcgatccggc tgtcgccgcg gatcgccgag gccatcgccc
     2461 agcccacggc ctgcgggaat tgggtcgcga ggttgcccga gatggtgaag aagccgtgcg
     2521 agttggaggc gtacatgatg ggcagctggc ggcccttcag cggatcgccg cggttcgaat
     2581 agacctggca catcatctga acgagcggat agtcgcgggc gacgagcagc ccctgctggc
     2641 ggtaggtggg gaagcacatg tcgtcgcggt cgagagcgtg ggcgccggcg accgcgatcg
     2701 cctcctcgcc cgtcgacttc atgtagaagc tggtcttgcc ctgcctctgg gcgcggtaca
     2761 ttcggtcgtc gtaggtccga accatcatca tgtcgcgaag gattcggcgc agggtgtcgg
     2821 ggtcgagctt cgggtcccag gggccgaccg cattcccctc gtcatcgagg acccggacca
     2881 gcgaatagac cagagcgtgg gtgtccgcgg cgggtgcggc ggaatcgggg cgcgggacgc
     2941 ttccggcggc gggaatctcg atatggctga aatcgacctc gtcccccggc cgcgccggcg
     3001 gctccgggac atatagggag agcgccggcc ggttcgggcg ctcgcttgct gtgttcatcc
     3061 ttggccctgt tcgttgcggc tcgctgcgcc cgtaacccgt gtcgccgccc ttgtgaagcg
     3121 gcgcccggcg gggcaggtaa agggtcaaag gagcgacacc atggccttgt tccgtccctt
     3181 cgccgctttg ctgctcctcg cggcctccgc ctgcgccact acggcgaagg atccgcccgc
     3241 gccgagctgg tacgtcatgc ggcatttgca gaaggccgaa gggcaggatc cggcgctgag
     3301 cgcggagggc cacgccaatg cacggcggct gatcgacttc ttcgccgccg acccgccgcg
     3361 ggctatctac gtcagcacca ctcggcgcgc gctggagacg gcgggtccgc tggcgtcccg
     3421 gctgggggtg acggtgaagg actatgatcc gcgcgacacg gccggactgg tggcgagggt
     3481 gaaggccgag gctggaacgg tgctcgtggt cggccacggc aatacggtgc cggagatcgt
     3541 ggcccagctc ggcggcgcac ggccggggga actgaccgag acggatttcg gcgacatctt
     3601 ccgggtgagg cgcgacggca aggtggagcg tctcaggctg gagacggcgc cttaaatcct
     3661 ccccggcatt tgccggggag ggggaccgcg cgaagcgcgg tggaggggtt cttcagactc
     3721 tggaagaccc ctccaccatg cttcgcatgg tccccctccc ccgcaaaggc gggggaggat
     3781 tttcgcttca aagatcgatg caggcggcga ggctctcgag cttgcggatg cgctccttca
     3841 ggtcggcaag ctcgatccgt tcggccattc cgccgtggcc cctcggctcg gcgccggcaa
     3901 gctcgagccg cttcaggtcg agccagccct gccagccctt cgaagcggcg aagagcagga
     3961 tgccgagcgc ggtcaggccg aagccggcca tcgccatcat cgtcatcggt tcgctcatcg
     4021 gatcctccct tctctctctg gttttcagcg caggctctcg atctcgcggg cgagaggcgt
     4081 gttctgagcg gttacgtagg tctcgatctc ggccatgcgg cggtcgatgt cgcgcatcga
     4141 gcggttgagc tcgtaggtgg aggtgcgcag gggccggtcg agggccgctt cgccatagcc
     4201 gtctacgggc cgtttcttgc cgatcatcgc cgccacgcca tagacgagca gcgcccaggg
     4261 gaaggtgatg agcgtcagga cgaccatcgc cactcggacg aatgtcgggt cccagttcat
     4321 gtgattggcg atgccggcgc agaccccgaa gaccttgcct tcttccttgt cgagatagaa
     4381 acgcttcttc atctttccct cctgagtgtc tcgagttcga ggcggggctc ccggctcccg
     4441 gtcgccgccg ggatgctcga gagggtccgc caattggggt tgcgggcgtt catgatccgg
     4501 tagatccagt cttccacggc tcatccctcc cggtccgccg cgagccgggc cttgagggcc
     4561 tccagctcgg cgtcgatctt ttcggcattg cgaagctcgg cgatctcctc gtcgaggctc
     4621 ctgggcgggg ctccgagcat cagcgcgtcg gcccggcctt cggcatgatc cacctcgcgc
     4681 tcgagcctgt cgaagcggga gaaggcctcg tcgaccctgg ggccggcggt catctcgcgg
     4741 aggcgggcgc gttggttcgc gctctccagc cgggtccggg tcgcgctctg ccgaatgcgg
     4801 gcttcgcgca acttgccctg gagcctggcg atgtcggctt ccgaggcacg catcgcctcg
     4861 tcgagcaggt cgatctcggt gcgcaggctc tcggcaagct ccgccgcctt ccgcttctcg
     4921 gtgagagcgc cgtgggcgag gtcctcgcgg cccttgccga gcgcgagctc cgctttctcg
     4981 gcccagctct cctggagcct gtcgaggcgg gcgatctgcc ggtgcatctc cttctggtcg
     5041 gccatggtcc gggcagtgct ggcccggacc tcgacgaggg tctcctccat ctcgaggatg
     5101 atcatccgga tcatcttcgc cgggtcctcg gccttgtcga gaagatccga gaaattggcg
     5161 gcgacgatgt cgcgggtgcg ggagaagatg ctcatcggca ggaactccat gaaaccgaaa
     5221 gccgggccag ccgggttgtg cgggttgcgg ggaccgcgga gcgcctcgtg gcggcgcggg
     5281 ccgccgggcc tcggcgggat ccgaaccagc ttcccggcgg gcacgtggat cgtctcagtc
     5341 acg
//
LOCUS       DASWNU010000002         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_58,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000002 DASWNU010000000
VERSION     DASWNU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGB57_00040"
     CDS             <1..546
                     /locus_tag="VGB57_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY0129763.1"
                     /translation="ETFEEGEQPANIIMAAAQGAATGVKLAVAVGAMVLAFVALVALA
                     NGLLGGVGGWFGYPDLSFQRLVGYIFQPVMFLIGIPWDQAGEAGGMFGTKIVLNEFVA
                     FIDLGNMEGSTLSPRSVAIVTFALCGFANFSSIAIQMAVTGGLAPNQRPVIARLGIKA
                     LIAGSLANLMSAALAGLLLPV"
     gene            595..1548
                     /locus_tag="VGB57_00045"
     CDS             595..1548
                     /locus_tag="VGB57_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY0129764.1"
                     /translation="MSTLAEPRPQVASVSLTASDTDPEGFAKELGESFERFGFAIIAD
                     HGIPADLIQRAEEKARAFFALPEEVKRAYHVAGGGGARGYTPFGIETAKGATAYDLKE
                     FWHVGRELPPGHKYSDSMAPNVWPEEVQSFRDTFLELFAAFDQAGLRILQAIARHLGL
                     DPDYFRDTVQDGNSVLRLLHYPPIPGDGPNVRAGAHEDINTITLLLGAEEAGLELLDR
                     DGNWLPVSPKPGELAVNIGDMLQRLTNNVLRSTTHRVVNPPPERRGKSRYSMPFFLHF
                     RPDFLIETLPDCVSEDRPNLYREPITAHEFLQQRLREIKLA"
     gene            complement(1535..1981)
                     /locus_tag="VGB57_00050"
     CDS             complement(1535..1981)
                     /locus_tag="VGB57_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0129765.1"
                     /translation="METGRFLLRTVLTGLNQFRRAFWALTKRGGRGVLAVPLTPAGKL
                     VLVRLTYAGGWHLPGGGFGRKEDPEAAALRELAEEIGMTGHGEVSRLDPIGSSLMFVV
                     RDVVYAPRRTFEIEEVAAFAPDALPSGTTRSAESGLAEALKLIRRA"
BASE COUNT          364 a          784 c          716 g          360 t
ORIGIN      
        1 gagacgttcg aggaaggcga gcagcccgcc aacatcatca tggccgcggc ccagggagcg
       61 gccaccggcg tcaagctcgc ggtcgcggtc ggcgcgatgg tgctcgcctt cgtcgccctg
      121 gtcgccctcg ccaacggcct gctcggcggg gtgggcggct ggttcggcta tccggacctc
      181 agcttccagc ggctggtcgg ctatatcttc cagcccgtca tgttcctcat cggcatcccc
      241 tgggaccagg ccggggaggc cggcggaatg ttcggcacca agatcgtgct caacgaattc
      301 gtcgcattca tcgatctcgg caatatggag gggagcacgc tcagcccgcg ctccgtcgcg
      361 atcgtgacct tcgcgctgtg cggcttcgcc aacttctcct ccatcgccat ccagatggcg
      421 gtgacgggag gcctcgcgcc caaccagcgg ccggtcatcg cgcgcctggg catcaaggcg
      481 ctgatcgccg ggtcgctcgc caatttgatg agcgccgcgc tggccggcct gctgcttccg
      541 gtgtgaacgc gtccgctagg tgacgacgcc cccgcttcgg agtaaagtgg ctagatgagc
      601 acactcgccg aaccccgccc gcaggtcgcc tcagtctccc tgaccgcttc cgacaccgac
      661 cccgagggct tcgccaagga gctcggcgaa tcattcgagc gcttcggctt cgcgatcatc
      721 gccgatcacg gcatccccgc cgacctcatc cagcgcgcgg aggagaaggc cagggccttc
      781 ttcgcgctgc ccgaggaggt gaagcgcgcc taccatgtcg ccggcggcgg cggtgcgcgc
      841 ggctacacgc cgttcggaat cgagaccgcc aagggcgcga cggcctacga cctcaaggaa
      901 ttctggcatg tcggccgcga gctgccgccc ggccacaaat atagcgattc gatggcgccc
      961 aacgtctggc ccgaggaagt gcagtccttc cgcgacacct tcctggagct gttcgccgcc
     1021 ttcgaccagg ccggccttag gatcctccag gcgatcgccc gccatctggg cctcgatccc
     1081 gattatttca gggatacggt gcaggacggc aattcggtgc tccgactgct tcattacccg
     1141 ccgatccccg gcgacggccc aaacgtgcgg gccggcgcgc acgaggacat caacaccatc
     1201 accctgctgc tcggcgccga ggaagcgggc ctcgagctgc tcgaccgcga cggcaactgg
     1261 ctgccggtca gcccgaagcc gggcgagctc gccgtcaaca tcggcgacat gctgcagcgg
     1321 ctcaccaaca acgtgcttcg ctcaaccacc caccgggtcg tgaacccgcc gcccgagcgg
     1381 cgcggcaaat cgcgctactc gatgcccttc ttccttcatt tccgcccgga tttcctgatc
     1441 gaaaccctgc ccgactgcgt ctcggaggac cggccgaacc tctatcgcga gccgatcacc
     1501 gcccacgaat tcctccagca acgcctgcgc gagatcaagc tcgcctgatc agcttcaggg
     1561 cctccgcgag gccgctctcg gcggagcggg tagtgcctga cggcagcgcg tccggagcga
     1621 aggcggcgac ctcctcgatc tcgaaggtgc gacgcggcgc gtaaacgacg tcgcgcacga
     1681 caaacatgag cgagcttccg atcggatcga ggcggctgac ttcgccgtgg ccggtcatgc
     1741 cgatctcctc ggcaagctcc cgcagcgcgg ccgcttccgg atcctctttg cgcccgaagc
     1801 ctccgccggg aaggtgccag ccgcccgcat aagtgagccg taccaggacc agcttgcccg
     1861 ccggggtgag cggcaccgca aggacgccgc ggccgccccg cttggtcagc gcccagaaag
     1921 cgcgacggaa ttggttcagc ccggtgagca cggtacggag gaggaagcgg ccggtttcca
     1981 catgtcgggc atagttgacg cagtggaaag agcggaatag tttctctttg aaaccggttg
     2041 aggagcggca gtggagacca gctacggcct tgccgacgcg gagacgctgc ggcgtctgac
     2101 cggcctcgaa tttttgcgcg cgatgatcgc cgggcgggtt cccgcgccgc cgatggcgga
     2161 gcgtctcggc ttccgcctcg tcgaggccga cgaaggccgg gcggttttcg aaggggcgcc
     2221 cgat
//
LOCUS       DASWNU010000003         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_77928, whole genome shotgun sequence.
ACCESSION   DASWNU010000003 DASWNU010000000
VERSION     DASWNU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77928"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VGB57_00055"
     CDS             complement(<1..1023)
                     /locus_tag="VGB57_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0129766.1"
                     /translation="MKSPKMNVAAVRAEFPILARRVRGKPLVYLDNAATSQKPRAMIE
                     RIDRIYRHEYARVEEGHTLSSEATKAFEGTRKKVARLLNAAEPREIVFCRGATEALNL
                     VSRMILHRGLAAGDEILITEMEHHSNIGPWIQVCEETGATLKVVPITPSGDLDLDRLE
                     TLLGERTKILAVSHVSNVTGAITPVKRITAMAHAKGIPVLVDGAQAVPHFPVDVRDIG
                     CEFYAGTGHKMGGPSSVGFLYGRADVMEKMPIADSGSTMSEDMTFEKITPKPLPHKYE
                     AGEPPFSEVEAWGPALDYWTDIGLDRIEAYERELTEYARSLLQSIPSVRILGDPADRI
                     SVVSFVA"
     gene            1246..2064
                     /locus_tag="VGB57_00060"
     CDS             1246..2064
                     /locus_tag="VGB57_00060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEY0129767.1"
                     /translation="MSVRVLVGVRSLRLLLSCFAFPFLLVSAAPPAVPAPSPRRPLVA
                     VIDSGVARTPELAPWLVAEYDTASAPGRPAFRPRYDHGTMVATILVREARRAVDILSF
                     RIDDPAGCPAHLQPPCQTSPGPVADAIRKAAALGVDAINLSLTLQDDPAIVEAIRDAA
                     ARNIRVVIAAGNEGLDRPGNLPMARAGYPNAVLVGAVDSEGRPWLGTNRPDGTHGDYV
                     YAWQRGVDVPTVAAKGAPIRGTGTSFAAPAETARIIARLGAGASSEAGTPSTAW"
     gene            complement(2140..>3752)
                     /locus_tag="VGB57_00065"
     CDS             complement(2140..>3752)
                     /locus_tag="VGB57_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0129768.1"
                     /translation="VAADPRTSSPEALATFARASIKALLNVPLIKDGRLVAVLGVHSG
                     REHDWKPEEVAVAEEVAERTWAAVGRARAETALRKSEERLRGAFEIDTVAVLFWGSDF
                     KLREANSAFLAMTGFSHEEAIGLTWQELTPPEFHPASEWAVAQIESTGWAEPYEKQYY
                     RKDGSRWWALFAPRRLADGDVVEFVLDVSDRRRKEQALRESEEQLRLIVGAARDYAIF
                     TTDPEGRISGWYAGAENVFGWSAEEAIGAPVDMTFTPEDREKGVPEGERATARDEGRA
                     PNVRWHVRANGSRVFIEGSTVALRSETGELTGFLKIGQDVTARMQAQEHERLLLFELQ
                     HRVRNTLAVVRSIVARTASASATVDEMESHLGGRLAAFSRVQATVTRNPGAGVDLNAL
                     VQDELLAHATREGPRLRVSGSEVLLQPKAAEGFSLAIHELATNAVKYGALAGDSGRLE
                     VSWSVEGEPGAEELQFEWVERDVAVAAGGGHEGFGTELLLRSLPYELDAETRLEVGSR
                     GVRFEMKVPADRALSPPKAPVGAEGGAG"
BASE COUNT          559 a         1339 c         1219 g          635 t
ORIGIN      
        1 ggcgacgaag ctcaccaccg aaattcggtc ggcgggatcg ccgaggattc gaacgctcgg
       61 aatcgattgc agcagcgagc gcgcatattc ggtcagctcg cgctcatagg cttcgatcct
      121 gtcgagcccg atatcggtcc agtaatccag cgccggcccc cacgcctcca cctcgctgaa
      181 cggcggctcg cccgcttcat atttgtgggg aagcggcttg ggcgtgatct tctcgaaggt
      241 catgtcctcc gacatggtcg aacccgaatc cgcgatcggc atcttctcca tcacgtcggc
      301 gcggccgtaa aggaagccga cgctcgacgg accgcccatc ttgtggccgg tgccggcgta
      361 gaattcgcag ccgatgtcgc gaacgtcgac cgggaagtgc ggcaccgcct gggcgccgtc
      421 gacgagcacc ggaatgcctt tggcgtgcgc catggcggtg atccgtttca ccggcgtgat
      481 cgcgccggtg acgttcgaga cgtgcgacac ggcgaggatc ttcgtgcgct cgcccagcag
      541 ggtctcgagc cggtcgaggt ccaggtctcc cgaaggcgtg atcggcacca ccttgagagt
      601 cgcgccggtc tcctcgcaga cctgaatcca cggtccgatg ttggaatgat gctccatctc
      661 ggtgatcagt atctcgtcgc cggccgcgag accccgatgc aggatcatcc gcgagacgag
      721 gttcagcgct tcggtcgcgc cgcggcagaa gacgatctcg cgcggctcgg ccgcgttcag
      781 aagccgggcg accttcttgc gagtcccctc gaacgccttg gtcgcctcgc tgctgagcgt
      841 atgcccttcc tcgacccggg catattcgtg ccggtagatc cggtcgatac gctcgatcat
      901 ggcccggggc ttctggctcg tcgcggcatt gtcgagatag acgagcggct tgccgcgcac
      961 ccgccgggcc aggatcggga attcggcccg aacggccgcc acattcatct tcgggctctt
     1021 catctcattc tccaagcccc cccgccatga acggagacaa cctcgcggtg ctgcgcctgt
     1081 tccgcccgct tcaaggcgct ttccgacgcc atgaaaaagc ttcgccaggg cctgtcgcca
     1141 acttgcaaac ctgtgttata aaccgacatt atctttggac gcccatttgg gcggggattg
     1201 gggacaagcg cacgcacccg agcggatcgc tccggtgccg cctcgatgag cgtccgggtc
     1261 ttggtcggag tccgctcgtt gcgtctgctt ctctcctgtt tcgcgttccc gttcctgctc
     1321 gtttccgccg cgccgccggc ggtcccggct ccgtcgccgc ggcgcccgct cgtcgcggtt
     1381 atcgacagtg gggtagcccg gacgcccgag ctcgctccgt ggctggtcgc ggaatatgac
     1441 accgcgtccg ccccggggcg gcctgccttc cggccgcgat acgatcacgg cacgatggtc
     1501 gcgacgatcc tggtgcgcga ggcgcggcgg gcggtcgaca tcctgtcgtt ccggatcgac
     1561 gaccccgccg gctgcccggc ccacctccag cccccctgcc agacctcgcc ggggcccgtc
     1621 gccgacgcga tccgcaaggc cgcggctctg ggggtcgacg cgatcaacct gtccctgacc
     1681 ctgcaggacg acccggcgat cgtcgaagcg atccgcgacg ccgccgcccg caatatccgg
     1741 gtggtcatcg ccgccgggaa cgaaggcctc gaccgtccgg gcaatttgcc gatggcgagg
     1801 gccggctatc cgaacgcggt gctggtcggc gcggtggatt ctgagggacg cccgtggctc
     1861 ggcaccaacc ggccggacgg aacgcacggc gactatgtct atgcctggca gagaggcgtc
     1921 gacgttccga cggtggcggc gaagggcgcg ccgatccggg ggacgggaac ctccttcgcg
     1981 gccccggccg agacggcccg cataatcgcc cggctcggcg cgggggcttc aagcgaggcc
     2041 gggacgccgt cgaccgcctg gtgactccaa cgggagcggc gcctggggga atcgtcagcg
     2101 acgtcggctg gcgatctcga tcaacgcacg ggccctagat caacccgcac cgccctccgc
     2161 gccgactggc gctttcggag ggctgagcgc tcggtcggcg ggcaccttca tctcgaaccg
     2221 cacccccctg ctcccgacct cgaggcgagt ctcggcgtcc agctcataag gaaggctgcg
     2281 caagaggagc tccgtgccga acccttcgtg gccgccgccg gcggccaccg cgacgtcccg
     2341 ttcaacccat tcgaactgca gctcctccgc tcccggctca ccttcgacgc tccaactgac
     2401 ctcgaggcgc cccgaatccc cggccaaggc gccatatttg acggcgttgg tggcgagctc
     2461 gtggatcgcg aggctgaagc cttcggccgc cttgggctgg agcaggacct cgctgccgct
     2521 gactcgcagc ctcggcccct cgcgggtggc atgggcgaga agctcgtcct gcaccagtgc
     2581 gttgaggtcg actccggcgc cgggattgcg ggtgacggtc gcctgcacgc gcgagaaagc
     2641 ggcgaggcgc ccgccgaggt gggattccat ctcgtcgacg gtggcgctgg ccgaagcggt
     2701 gcgggcgacg atcgacctca ccaccgccag cgtgttgcgc accctgtgct gcagctcgaa
     2761 cagcagcaat cgctcatgct cctgcgcctg catccgggcc gtgacgtcct ggccgatctt
     2821 caggaagccg gtgagctcgc ccgtttccga ccgcagcgcc acggtcgagc cttcgatgaa
     2881 aacacgcgag ccgtttgcgc ggacatgcca gcggacgttc ggagcgcggc cctcgtcgcg
     2941 cgccgtcgcc cgctccccct ccgggacgcc tttttcccgg tcctccggcg tgaaggtcat
     3001 gtcgacgggg gcgccgatgg cttcctccgc cgaccagccg aacacattct cggcgccggc
     3061 ataccagccg ctgatccgtc cctccggatc ggtggtgaag atcgcatagt cgcgcgcggc
     3121 gccgacgatc agccgaagtt gctcctcgct ctcccgaagc gcctgctcct ttcgacggcg
     3181 gtcgctgacg tcgaggacga actccacgac atccccatcc gcgaggcggc gcggggcgaa
     3241 gagcgcccac cagcgcgagc cgtccttccg gtaatattgc ttctcgtacg gctccgccca
     3301 gcccgtgctc tcgatctgcg ccaccgccca ttcggaagcc gggtggaatt caggcggggt
     3361 cagctcctgc caggtaaggc cgatggcctc ttcatggctg aagccggtca tcgcgagaaa
     3421 agcggaattg gcctcccgca gcttgaagtc ggagccccag aacaggacgg cgacggtatc
     3481 gatctcgaat gcgccgcgga gccgctcctc gctcttgcgc agcgcggttt ccgcccgcgc
     3541 acgccccacc gccgcccagg tgcgctcggc gacctcctcg gcgaccgcca cctcttcggg
     3601 cttccagtca tgttccctgc cgctgtggac cccgagaacg gcaaccagac gcccgtcctt
     3661 gatcagcggg acgttgagga gcgccttgat cgaggcgcgc gcgaatgtcg ccagggcttc
     3721 cggggacgag gttcggggat cggccgccac gt
//
LOCUS       DASWNU010000004         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_129832, whole genome shotgun sequence.
ACCESSION   DASWNU010000004 DASWNU010000000
VERSION     DASWNU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129832"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(483..1586)
                     /gene="aguA"
                     /locus_tag="VGB57_00070"
     CDS             complement(483..1586)
                     /gene="aguA"
                     /locus_tag="VGB57_00070"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEY0129769.1"
                     /translation="MGRTLKSTPAEDGFRMPGEFEPHAGCWMAWPERPDNWRLGAKPA
                     QEAYAAVAEAIASSEPVTMAVSDAQFENCRALLSPAVRVVEVSTDDAWIRDHGPTFVV
                     DGDGGRRGVDWRFNAWGGTEGGLYFPWDHDERVAAKVLEVEGDERYRAPIVLEGGAIH
                     VDGEGTVLTTEECLLNPNRNPELSREQIKRVLLDYLGAEKVIWLGAGVFEDETDGHVD
                     NLACFARPGGVLLTWSDDEDDPQHAISRDARERLEAATDARGRSLEVVLLPSPGPLQI
                     SAEEAEGVDAGEGTVPRRAGDRMAASYVNFYLANSHLVLPLLDPRHDEEAAAILRRTL
                     PDREVVGVPAREILLGGGNIHCITQQVPAVSAR"
BASE COUNT          283 a          603 c          602 g          286 t
ORIGIN      
        1 tcccgaccat cgaccgctac gtacccggca gccgcgtcca cctgattccg ttggagcaga
       61 tcgccgagac caacagcgat cacttcgccg tctcgccacc ctggctgaag gatgtgtggt
      121 gggatccgga ggccgtggga actagttagg gcgtgaatgg taaagcagac aaaaaacatc
      181 agctagaaga aagaatggtg taatgcaaca gagtggtcga ccagtatcta gcgtccgttt
      241 gcaggcgcta taggaaagct gatcgaatga attatttttt gaaaactaaa cacctcacat
      301 cggatcacag cattgcgcta caagtcgcct gggggcgggc agctgccgcc cgtcgagctt
      361 tcctcggcga gtggatgtgg cctcggtgtt gcagcaccga gaagttcggg taacccaaag
      421 tggccgttct agtccgcccc ccagagtgtt ccaaatccct tggtcgcggc tcgaccgtct
      481 ggctaacgag cactaaccgc gggcacctgc tgggtgatgc agtggatgtt gccgccgccg
      541 aggaggatct cccgcgccgg cacaccgacg acttcgcggt cggggagggt gcggcggagg
      601 atcgccgccg cctcctcgtc gtggcggggg tcgagaaggg ggaggacgag gtgggagttg
      661 gcgaggtaga agttgacgta ggaggcggcc atacggtctc cggcgcggcg cgggaccgtg
      721 ccctcgccgg cgtcgacgcc ctcggcctcc tcggctgaga tctgcagcgg gccgggcgag
      781 gggagcagga ctacctccag ggagcggcca cgcgcatcgg tcgccgcttc caggcgctcg
      841 cgcgcgtcgc gggagatggc gtgttgggga tcgtcctcgt cgtcgctcca ggtcagcagc
      901 acccctcccg gtcgggcgaa gcaggcgagg ttgtcgacgt gcccgtcggt ctcgtcctcg
      961 aagacgcccg cccccagcca gatcaccttc tcggcgccga ggtagtcgag caggacccgc
     1021 ttgatctgct cgcgggacag ctcgggattg cggttcgggt tgagcaggca ctcttcggtc
     1081 gtcagcaccg tgccctcgcc gtcgacgtgg atcgcgccgc cctcgagcac gatcggcgcc
     1141 cggtagcgct cgtcgccctc gacctccagc accttcgccg ctacccgttc gtcgtggtcc
     1201 caggggaagt agagaccgcc ctcggtcccg ccccaggcgt tgaagcgcca gtcgacgccg
     1261 cggcgacccc cgtcgccgtc gacgacgaag gtcggcccgt ggtcgcggat ccaggcgtcg
     1321 tcggtggaga cctcgaccac gcgcaccgcc ggcgacagca gcgcccggca gttctcgaac
     1381 tgggcgtcgg agaccgccat cgtcaccggc tcggaggagg cgatcgcctc cgccaccgcc
     1441 gcgtaggcct cctgcgccgg cttggccccc agccgccagt tgtccggccg ctccggccag
     1501 gccatccagc agcctgcgtg gggctcgaac tcgccaggca tccggaatcc gtcttcggcg
     1561 ggcgtcgatt tcagcgtgcg gcccatctcc gtctatcttg aaccagtgga cgagatccgc
     1621 ctgatccatg ccggggatcc ccccgagtcg cctgcccgga cccgcccgcc ggaccgcccg
     1681 ccgttccgga tcgccgcggt ccagcaccgc tggcaccggg atccggccga gcacgaggcg
     1741 gcgctggagg aggggatccg cctcgcggcc gagg
//
LOCUS       DASWNU010000005         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_207714, whole genome shotgun sequence.
ACCESSION   DASWNU010000005 DASWNU010000000
VERSION     DASWNU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207714"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..627
                     /locus_tag="VGB57_00075"
     CDS             277..627
                     /locus_tag="VGB57_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129770.1"
                     /translation="MAGTRVERAMSLALLLALQAAPEARAEPLPIEFDLATVKPADPC
                     RTGGGGSDIVVCGRRPRGNFDFEKWEREFRTGPLVAETSIGRGAVARAYGESVGMPGG
                     QISKRAMVGIGLKF"
     gene            complement(1012..1251)
                     /locus_tag="VGB57_00080"
     CDS             complement(1012..1251)
                     /locus_tag="VGB57_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129771.1"
                     /translation="MGTEGRRRDKAEFRDGPEEVLACLEAAFEDGDPEAIAAALTNAA
                     RAKGLAQDPLTARSDIASVVGTVNALGLKLAVKAA"
     gene            1305..1682
                     /locus_tag="VGB57_00085"
     CDS             1305..1682
                     /locus_tag="VGB57_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129772.1"
                     /translation="MRGQAVLRAAVLPGLLGDGEVELEHQRRQVPGREPKQPADGREA
                     EPVEPLEPFLQRVGGKLAPLRRQEPLLRLESRHPGLGPPQGRDRPLQASPGRAPTRAR
                     RSRKRDRRPTTAPGDGASADTHA"
     gene            1749..2048
                     /locus_tag="VGB57_00090"
     CDS             1749..2048
                     /locus_tag="VGB57_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0129773.1"
                     /translation="MVLTTKKLVKGDFLTRPVLLPVQCRMARVALQWSVNEAGRAAGV
                     SYRTIVRLENEQRDIQADKLAAIRRGFEEAGVRFVDVGPDAGAVVPPPLKVVSPQ"
BASE COUNT          362 a          775 c          787 g          342 t
ORIGIN      
        1 gggcggagtc gagaccgctc cgggccgtat cggcggagcg acgccgggcc tcggcgacgg
       61 agataccggc gaagcgggcg ctccggtggc ggtgtcgatc accgacagcc gtccgtcctt
      121 ctcctcgacc cgacatcgtc ccttgggcgc gtccatcggc gctcccgggt cgagcgtaac
      181 gcaagtgcgg cgggagcgga agacgcgagc ggagccgggc ggattacgcc ggggcgcgcg
      241 acggctgcct tatcttcgcc ggaaatggcg gggaggatgg cgggcacccg agtggagcgt
      301 gcgatgagcc tggccttgct gcttgccctt caggccgcgc ccgaggcgcg ggccgagccg
      361 ctgccgatcg agttcgatct cgcgacggtg aagcccgccg acccctgcag gaccggcggg
      421 ggcggctccg acatcgtggt gtgcggccgc cgcccgcgcg gcaacttcga cttcgagaaa
      481 tgggagcgcg agttcaggac cggcccgctc gtcgccgaaa cctcgatcgg ccgcggcgcc
      541 gtcgcccgcg cctatggcga aagcgtgggg atgccgggcg gccagatcag caagcgggcg
      601 atggtgggga tcgggctcaa attctgaacg gggttccacg tggaacgttt catgaacatt
      661 aatcttcgca aaggtggtcg cgacggttgg aagcttcgaa aagcgaccca ccaccgcctt
      721 tgtccgctgt ggcaagtctt tgtttaaaaa gaaaaacagg ttgcgcctta ttcagctcct
      781 taccgatcgc aatcgcgagg aatcgcccgg aatcgcgatt aatcgccaca tttcgcgcca
      841 agcgcgagaa gcgcaggttt cggccggtcc tcgaaaggga ccgaccgccg ccgacttcgc
      901 atcggacgtg cgctcgcccg gttctagagg gaagacacga aggcgcgaag gcacaacgta
      961 gaaaaagaga ggatggccgc ttcgcggcgg aaggtcgcgg gttcccggcc ttcaagcggc
     1021 cttgactgca agcttcagcc ccagggcgtt cacggtcccg acgacggagg cgatgtcgct
     1081 tcgcgcggtc agcgggtctt gcgcgaggcc cttggcccgg gccgcgttgg tgagggccgc
     1141 agcgatcgcc tcggggtcgc catcctcgaa cgcggcctcg agacaggcga gaacctcttc
     1201 cggaccgtcg cggaattcgg ccttgtcccg gcgccttcct tcagtcccca tttgcgggcg
     1261 cccagggagc ggcgtccgcc gaaatccgcc gggcgtcggc ggcgatgagg ggccaggcag
     1321 ttctccgagc cgctgtcctc ccaggcctgc tcggcgacgg cgaggtcgag cttgagcacc
     1381 agcgccggca ggtcccgggc cgggagccga agcagccggc ggacggccgc gaggcggagc
     1441 ctgtcgagcc gctcgaacct ttcctccagc gcgtcggcgg caagctcgcg ccgctccgcc
     1501 ggcaagagcc tctcctccgc ctcgaaagcc gccacccggg cctcggcccg ccgcagggcc
     1561 gcgaccgccc gctccaagct tcgccgggac gagctccgac ccgggctcgc cgcagccgca
     1621 agcgtgaccg ccgccccacg accgcccccg gcgacggcgc aagcgccgac acccacgcct
     1681 agcaacgccc ggcgcgacag gttatgcctc ccggcagcca tgcctgaggg tctccttctt
     1741 cgtggtcagt ggtactgacc acgaagaagc tggtcaaggg ggattttctg acgcgccctg
     1801 tgctcctgcc cgttcaatgc cgaatggccc gggtcgcgct gcaatggtcc gtgaacgaag
     1861 cgggacgcgc ggccggggtt agctaccgga ccatcgtccg cctagagaac gagcagcgcg
     1921 atatccaggc cgacaagctt gcggcgatcc ggcgcgggtt cgaggaggcc ggagtgcgtt
     1981 tcgtcgacgt gggacccgac gccggcgcag tggtcccgcc accgctaaaa gtcgtgtccc
     2041 ctcaataggt ggtcatcgac gctgaccgcg cagatcgctc gcggaggcac gcggccgccc
     2101 cttggacatc gaccggcctg gttgcgcctg gcccgggtga cggtggcggg ggtaaggaaa
     2161 gtgacagtca ctagtgactg tcactcgcgc ccatagtgac tgtcactcgc gccttgtcac
     2221 tcgcgccttg tcacctgcgc ccccatccgg cggcgatggc cctcgc
//
LOCUS       DASWNU010000006         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_181812, whole genome shotgun sequence.
ACCESSION   DASWNU010000006 DASWNU010000000
VERSION     DASWNU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181812"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGB57_00095"
     CDS             <1..379
                     /locus_tag="VGB57_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEY0129774.1"
                     /translation="LLRLLDLPDGVRQLVESGQLSMGHARALIGAADAEALARDAVER
                     GLSVREVEKLARFGKPGRERQGRLEIKGAGADIEALERQLGDMLGLKVKITHRPDGGV
                     VALNYSTLDQLDMICQRLSGEKI"
     gene            complement(466..1488)
                     /gene="holA"
                     /locus_tag="VGB57_00100"
     CDS             complement(466..1488)
                     /gene="holA"
                     /locus_tag="VGB57_00100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0129775.1"
                     /translation="MKAKRAEMERALRSPSAYRFFLLYGPDDSGSHSLVKVAAAGAGA
                     EAERVELTGAELKADPARLADEAASISLFGGARYIVVQPAGDECLAAVEALLQAPAAG
                     NPVLLIAGALKPTSKLLKLALAEPAALALASYAPEGQEAERLVSDMARAEGLAVRPDV
                     ARRLADACAGNRAILALELQKLALYLDAAPDRPRPLDHEALDAVGAAAEEGDLSRLVD
                     SVGNGNAAMLQSELVRLSSEGIEGIPLIRAVLRRMSLLSRLRAQVERGSSPEAVMASQ
                     GKSLFWKEKDSIGRQLRSWRSDALARSVSRLLEAERQVKASGGLGPTAVDEELFAICR
                     QAARLR"
     gene            complement(1485..1976)
                     /gene="lptE"
                     /locus_tag="VGB57_00105"
     CDS             complement(1485..1976)
                     /gene="lptE"
                     /locus_tag="VGB57_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY0129776.1"
                     /translation="MKRLLPLTLLLAGCGLQPLYGGGASGPVAQSLRSVTVAPIEGRA
                     GWLVRTALEDRLGAPSAGAAAYRLEVLLDDEIAGFGIRRDDAVTRERRTLRARYRLVD
                     AARGTVLLDATAGSDAGIDVVGSEYATVAAEQTALERLSREVADQIVTRVALYARRSG
                     NAP"
     gene            complement(1973..4489)
                     /gene="leuS"
                     /locus_tag="VGB57_00110"
     CDS             complement(1973..4489)
                     /gene="leuS"
                     /locus_tag="VGB57_00110"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0129777.1"
                     /translation="MAARFNPLEADARWQKVWEEQGTFHASGDGAHPRAYVLEMFPYP
                     SGRIHMGHVRNYTMGDVLARYRRMNGFDVLHPMGWDAFGMPAENAAMEKKVHPGAWTR
                     SNIDTMRAQLKRLGFALDWSRELATCDPSYYGHEQALFLDLYEAGLVYRKQSEVNWDP
                     VDMTVLANEQVIDGRGWRSNALVERRKLSQWFLRISEFADELLDGLGRLDQWPDKVRL
                     MQENWIGKSQGMRFRFDLPEPVAGHSGFEVFTTRPDTIFGASFAAISPDHPIALALAE
                     GNEALAAFIAECKQGGTTAAELETAEKKGFDTGARVTHPLDAEWTLPLYVANFVLMDY
                     GTGAIFGCPAHDQRDLDFARKYGLPVTRVVSPSPEEADEPIGDEAYSGPGRLVNSRFL
                     DGLAAEDGIREVIARAEAEGWGEGTTVWRLRDWGVSRQRYWGTPIPIVHCEACGPVAV
                     RRDQLPIVLPEDIDFETPGNPLDRHPTWAKADCPDCGGPARRETDTLDTFVDSSWYFI
                     RFASQPADAPFDAEEAERWLPVDQYIGGVEHAILHLLYARFWTRALQRIGKLDVEEPF
                     QGLFTQGMVTHVTFQDAEGKWLTPDEAESYSGPVARGRVEKMSKSKRNTVDPEPIVDQ
                     YGADAVRWFMLSDSPPERDLEWSEAGIQGSWRFVQRIWRLAAEEAGRGSSTWLGGGED
                     DVALDRKVHRTIAAVGANIEALAFNKAVANLYELTNALEKAPASPSRSRAVETLIRLA
                     APMVPHLAEEAWTAMGQEGLIAEAEWPAADPALLVEEEVTIAIQVNGKLRDTLTAPKG
                     MAKDRLEEMALASEKVARILEGKPPRKVIVVPDRLVNVVA"
     gene            complement(4499..4972)
                     /locus_tag="VGB57_00115"
     CDS             complement(4499..4972)
                     /locus_tag="VGB57_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEY0129778.1"
                     /translation="MRRADILALTIVGALGLSACDFIRDDINKSLGGGSGNRVREADL
                     AASQVTTIGVNSYLWRAALETLSFAPMAQVDSNGGVMVTDWYSNPGSPNERVKVTATI
                     LDRDLRADALRIAASRQVLNGGQWVDAPVAAATVQKLEEIILTRARDLRRNAIAS"
     gene            5220..5930
                     /locus_tag="VGB57_00120"
     CDS             5220..5930
                     /locus_tag="VGB57_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0129779.1"
                     /translation="MKLGSIGAGGSAALVALSLMLTPALAASNQQARKARPAAALRAG
                     TFTPAVADPRLAAELARRGVQANSFRFTPAPTAVNRSQSIRVAIRARAATPAGALRRA
                     ADVSATPVTAITPTVYNLGASVGWKRFALSGDVDSVKGGTIPGGRKAAEVGVSYSGNR
                     FTARAEAGVAKADPATPRIIAEDESYTVGVGGSYRLARTLDVTGGVRYKIQRDRLEAL
                     TDPRRDSQAVYVGTAFRF"
     gene            complement(6040..6594)
                     /locus_tag="VGB57_00125"
     CDS             complement(6040..6594)
                     /locus_tag="VGB57_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0129780.1"
                     /translation="MPRRQPLPRLWLMTDERQGDELWEALERLPRGSGLVFRHYSLAP
                     AERRTLFERVRRVARRRRLLLLAAGKGLRGDGVHGGGRGGLRSASVHDLRELKTAERA
                     GATLVFLSPAFPTRSHPGARALGPMRFGLIAGQARVPVIALGGVDGRKARRLPHIYGW
                     AGIDAWLASPSKMGRGTMRSMVEG"
BASE COUNT         1024 a         2391 c         2327 g         1023 t
ORIGIN      
        1 cctgctgcgc ctgctcgacc tgccggacgg cgtccggcag ctcgtcgaga gcggccagct
       61 aagcatgggc catgcccgcg ccctgatcgg ggcggccgac gccgaggcgc tggcccgcga
      121 cgcggtcgag cgaggactgt cggtgcgcga ggtcgagaag ctcgcccgct tcggcaagcc
      181 cggccgcgag cgccagggcc ggctcgagat caagggtgcc ggcgccgaca tcgaggcgct
      241 cgagcgccag ctcggcgaca tgctcggcct caaggtgaag atcacgcacc gaccggacgg
      301 cggcgtcgtg gcgctcaatt attcgacgct cgaccagctc gacatgatct gccagcgcct
      361 gtcgggcgag aagatataag ccgtcatccc ggcgaaagcc gggacctcgg gacgagaagt
      421 cacggtgggt ctccgcgagc tcccggcttg cgccgggatg acgggctagc gcagccgcgc
      481 cgcctggcgg cagatcgcga atagctcctc gtccacggca gtgggtccca gtcctcccga
      541 ggccttcacc tgccgctccg cttcaagcag ccggcttacg cttcgggcca gagcgtccga
      601 gcgccagctc ctgagctgtc ggccgattga gtccttttcc ttccagaaca gcgacttacc
      661 ttgtgacgcc atgacggctt ccgggctgct tccgcgctcc acctgggcac gcaggcgtga
      721 cagcaggctc atccgtcgaa gcaccgcccg gatcagcggg attccctcga tgccctcgct
      781 ggagagtcgg accagctccg actggagcat ggccgcattg ccgttgccga cgctgtcgac
      841 gagtcggctg aggtcgcctt cctcggccgc ggcgccgacg gcgtcgaggg cctcatggtc
      901 gagcggcctg ggccgatcgg gcgcggcgtc gaggtacaag gccagcttct ggagctcgag
      961 cgcgaggatg gcccggttgc cggcgcaggc gtcggcgagt cggcgggcga cgtcggggcg
     1021 gacggcgagg ccctcggccc gggccatgtc cgagaccagc cgctcggctt cctgcccttc
     1081 cggggcgtag ctggccagcg ccaaagcggc cggctcggcg agggcgagct tgaggagctt
     1141 ggaggtcggc ttgagggctc cggcgatcag gaggaccggg ttgcccgccg ccggcgcctg
     1201 aagcagcgcc tccaccgccg cgaggcattc gtcgccggcc ggctggacga cgatgtagcg
     1261 ggcgcctccg aacagcgaga tggaggccgc ctcgtcggcg agccgggccg gatccgcttt
     1321 cagctccgcc ccggtgagct cgacccgctc cgcctccgcc cccgccccgg cggcggcgac
     1381 cttgaccagg gaatgggagc ccgaatcgtc ggggccgtag aggaggaaga agcggtaagc
     1441 cgaggggctt cgaagcgccc gctccatttc ggcgcgtttc gccttcacgg cgcgttgccg
     1501 ctccgccgcg catagagggc gacccgggtg acgatctggt cggcgacctc cctcgacagc
     1561 cgctccagcg ccgtctgctc cgccgcgacg gtcgcatatt cggagccgac cacgtcgatg
     1621 cccgcgtcgg agcccgcggt ggcgtcgaga aggaccgtgc cccgggccgc gtcgaccagc
     1681 cggtagcgcg cccggagagt ccggcgctcg cgggtgacgg cgtcgtcccg gcggattccg
     1741 aagccggcga tttcgtcgtc gagcagcact tcgagccgat aggccgcggc gcccgcgctc
     1801 ggagcgccga gccggtcctc gagcgcggtt cggaccagcc agccggcgcg cccctcgatc
     1861 ggagccaccg tcaccgagcg cagggactga gccaccgggc ccgaggcgcc cccgccatag
     1921 agcggctgca gcccgcagcc cgccagaagg agggtcagag ggagaagccg tttcatgcga
     1981 cgacgttcac cagccggtcg gggacgacga tcaccttgcg gggcggcttg ccctcgagga
     2041 tccgggccac cttctccgaa gcgagcgcca tctcctcgag cctgtccttc gccatgccct
     2101 tgggtgcggt cagagtatcg cgaagcttgc cgttgacctg gattgcgatc gtcacttcct
     2161 cctcgaccag cagggcgggg tcggccgcgg gccattcggc ctcggcgatg agcccttcct
     2221 ggcccatcgc ggtccaggcc tcctcggcga ggtgcggcac catcggcgcg gcgagccgga
     2281 tcagggtctc gaccgccctg cttcgcgacg gcgaggcggg cgccttttcg agtgcgttgg
     2341 tcagctcgta cagattggcc accgccttgt tgaaggccag agcctcgata ttggcgccga
     2401 cggcggcgat ggtccgatgg accttgcggt cgagcgccac atcgtcctcg ccgccgccga
     2461 gccaggtcga cgatccgcgc cccgcttcct cggccgcgag gcgccagatc ctctggacga
     2521 agcgccagct cccctggatc cccgcctcgc tccattcgag gtcgcgctcc ggggggctgt
     2581 cggagagcat gaaccagcgc accgcgtcgg cgccgtactg gtcgacgatc ggctccggat
     2641 cgaccgtgtt gcgcttcgac ttggacatct tctcgacccg gccgcgagcg accggcccgg
     2701 agtagctttc ggcctcgtcg ggcgtcagcc acttgccctc ggcatcctgg aaggtgacgt
     2761 gggtgaccat gccctgggtg aagaggccct ggaacggctc ctccacgtcc agcttgccga
     2821 tgcgctgcag ggcgcgggtc cagaagcggg cgtagagcag gtggaggatc gcatgctcga
     2881 caccgcctat atattggtcg accggaagcc agcgctcggc ctcctcggca tcgaagggcg
     2941 cgtcggcggg ctggctcgcg aagcggatga agtaccagga cgaatcgacg aaggtgtcga
     3001 gcgtatcggt ctcgcgccgc gccgggccgc cgcaatcggg gcaatcggcc ttcgcccagg
     3061 tcggatggcg gtcgagcggg ttgcccggag tctcgaagtc gatgtcctcg ggcaggacga
     3121 ttggaagctg gtcgcgccgg acggcgaccg ggccgcaggc ctcgcaatgg acgatcggga
     3181 tcggcgtccc ccagtaacgc tggcgcgaga cgccccagtc gcgcagccgc cacaccgtcg
     3241 tgccctcgcc ccagccttcg gcctcggcgc gcgcgatcac ctcccggatt ccgtcctcgg
     3301 cggcgagacc gtcgaggaat cggctgttca cgagtcggcc cggcccggaa taggcctcgt
     3361 cgccgatcgg ctcatccgcc tcctcgggcg agggcgacac gacccgggtc accggcaggc
     3421 cgtatttgcg ggcgaaatcc aggtcgcgct ggtcgtgcgc cggacagccg aaaatggcgc
     3481 cggtgccgta gtccatcagc acgaaattgg cgacgtagag gggcaaggtc cattcggcgt
     3541 cgagcgggtg ggttacccgg gcgccggtgt cgaagccctt cttctccgcc gtttccagct
     3601 ccgccgccgt ggtcccgccc tgcttgcact cggcgatgaa ggcggcgagc gcctcattcc
     3661 cttccgccag cgccagagcg atgggatgat cgggcgagat cgcggcgaag cttgcgccga
     3721 agatcgtatc cgggcgggtg gtgaagacct cgaaacccga atgaccggcc accggctcgg
     3781 gcagatcgaa ccggaagcgc atcccctggc tcttgccgat ccaattctcc tgcatcagcc
     3841 ggaccttgtc gggccattgg tcgagccggc cgagaccgtc gagcagctcg tcggcgaact
     3901 cgctgatcct gaggaaccac tggctgagct tgcgccgctc gacgagcgcg ttcgagcgcc
     3961 agccgcggcc gtcgatcacc tgctcgttgg ccagcacggt catgtcgacc ggatcccaat
     4021 tcacttcgct ctgcttgcgg tagaccagcc cggcttcata gagatcgagg aacagcgcct
     4081 gctcgtggcc gtaataggaa gggtcgcagg tggcgagctc ccggctccag tcgagagcga
     4141 agccgaggcg cttcaattgc gcgcgcatcg tgtcgatgtt cgaccgggtc caggctccgg
     4201 gatggacctt cttctccatc gccgcattct cggccggcat tccgaacgcg tcccatccca
     4261 tcggatggag gacgtcgaag ccgttcatcc ggcgatagcg ggcgagcacg tcgcccatcg
     4321 tgtagttgcg gacgtggccc atatggatgc gccccgacgg ataggggaac atctcgagca
     4381 cgtaggcgcg ggggtgggcg ccgtcgccgg aagcgtggaa ggtgccctgc tcctcccaca
     4441 ccttctgcca gcgcgcgtcg gcctcgagcg ggttgaaacg cgccgccatg tcagttcctc
     4501 agcttgcgat ggcgttccgg cggagatcgc gggcgcgggt gaggatgatc tcctcgagct
     4561 tctggacggt ggccgcggcc accggcgcgt cgacccactg gccgccgttc agcacctgcc
     4621 gcgacgcggc gatgcgcagc gcgtcggcgc gcaggtcgcg gtcgaggatg gtcgcggtga
     4681 ccttgacgcg ctcgttcggc gagccgggat tgctgtacca gtcggtgacc atcactccgc
     4741 cgttcgaatc gacctgggcc atgggcgcga aggagagcgt ctcgagcgcc gcgcgccaca
     4801 ggtaggaatt gaccccgatc gtcgtcacct gcgacgccgc caggtccgcc tcgcgcacgc
     4861 ggttgccgct gccgcccccg agcgacttgt tgatgtcgtc ccgaatgaag tcgcaggccg
     4921 agaggccgag ggcgccgacg atcgtcaggg ccagaatatc agcgcgtcgc atgaagcttg
     4981 tgtccttgga aactacaggg gtcgagcgcc gttatagaac gcgccacgca ggcggcaagc
     5041 cgtctctggg cgctaaggga gccaagtgcc ggggtcgagc gtattgctgc cacgtcaatt
     5101 cggggttgtg gaaaagatgc aacatgccgg aacgattcgt gtccgccccg gccgcttcgc
     5161 tggtttgacc gccacggacg tactacatcc gcttcgatac ggagtcgcct gaagtgattg
     5221 tgaagctcgg cagtatcggt gcaggaggaa gcgcagcgct cgtcgcgctg tcgctgatgc
     5281 ttacgcccgc gctcgccgca agcaaccagc aggccaggaa ggcgcgcccg gccgccgcgc
     5341 tccgcgccgg caccttcacc cccgccgtcg ccgatccccg cctcgccgcc gagctcgccc
     5401 gccgcggagt ccaggccaac agtttccggt tcacccccgc cccgaccgcg gtcaaccgca
     5461 gccagtcgat tcgggtcgcc atccgcgccc gcgccgccac tccggccgga gccctgcgcc
     5521 gagccgccga cgtctcggcc accccggtca ccgcgatcac gcccaccgtc tacaatctcg
     5581 gcgcctcggt cggctggaag cgcttcgcgc tttccggcga cgtcgactcc gtcaagggcg
     5641 gcaccatccc cggcggccgc aaggccgcgg aggtcggagt gagctattcg ggcaatcgct
     5701 tcaccgcccg cgccgaggcc ggagtggcca aggccgatcc cgcgactccg cggatcatcg
     5761 ccgaggacga gagctatacg gtcggagtcg gcggctccta ccggctcgcc cgcaccctcg
     5821 atgtcaccgg cggagtccgc tacaagatcc agcgcgaccg tctcgaggcg ctgacggatc
     5881 cgcgccgcga cagccaggcc gtctacgtcg gcaccgcctt caggttctga tctgcctccc
     5941 tatgcgaagc atggggaggg ggaccacacg tagtgtggtg gaggggtaga aggcgcaggc
     6001 ggcagcggat cgcacgcaaa cccaccaagg agacggcagc tacccctcca ccatgcttcg
     6061 catggtcccc ctccccatct tcgatgggga ggcaagccag gcgtctattc cagcccagcc
     6121 gtagatgtgg ggcaaccgcc gcgcctttcg tccgtccacg ccacccagcg cgatcaccgg
     6181 aacccgcgcc tggccggcga tcagtccgaa tcgcatcggt ccaagcgcgc gggcgccggg
     6241 atgcgatcgg gtgggaaagg ccggggagag gaagaccagc gtcgccccgg cgcgctccgc
     6301 cgtcttcagc tcgcgcaggt catggaccga agcgcttctt aatccaccgc ggccgccgcc
     6361 atgcaccccg tcgccgcgca gccccttgcc ggcggcgagg aggagcagcc gacggcgcct
     6421 ggccacgcgc cgaacgcgct cgaacagggt ccgccgctcg gccggcgcaa ggctgtaatg
     6481 gcggaagacc aggccggatc ccctcggcag ccgctcaagc gcctcccaca gctcatcgcc
     6541 ctggcgctcg tcggtcatca gccagaggcg cggcagaggc tggcggcggg gcatgccgct
     6601 tcctatagca gccgccatga ccggcgcatc acccgcagag cggcttcggg acgtaaggga
     6661 ggcgatcggg cgttccgccc gcctggcggc tcgcccggcg gatgacatca ccctgatcgc
     6721 cgtctcgaag acccacggcg ccgactcgat ccggccgctg atcga
//
LOCUS       DASWNU010000007         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_493139, whole genome shotgun sequence.
ACCESSION   DASWNU010000007 DASWNU010000000
VERSION     DASWNU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493139"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="VGB57_00130"
     CDS             complement(<1..144)
                     /locus_tag="VGB57_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY0129781.1"
                     /translation="MIETIFALSSGSPPAALAVVRISGTRAHSALEALAGRLPAPRVA
                     TLAT"
     gene            complement(249..482)
                     /locus_tag="VGB57_00135"
     CDS             complement(249..482)
                     /locus_tag="VGB57_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HEY0129782.1"
                     /translation="MKISVDVDCTPEEARRFLGLPDLTPVHEAYVQRMTRAASEGLTA
                     ESAAEMMKAWGPMSEAGMTMWRSMLEGMGGKKG"
     gene            551..1255
                     /locus_tag="VGB57_00140"
     CDS             551..1255
                     /locus_tag="VGB57_00140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0129783.1"
                     /translation="MGTIRIDTLEGEGGFDAYAAEPEGGAGPRAAIVVIQEIFGINEG
                     IRRKCDAWADKGYLALAPDLFWRLEPGVELDPDVPDEFRQALDLMGRFDQDQGVVDIE
                     ATIRAARGRLDEGAKVGIVGYCLGGRLAYMAAARTDADASVGYYAVGLPNILHEKNAI
                     ARPLMLHIAGADHFVDEAAQRAMHEALDDHPRVTLHDYPGEDHGFAAEMGKRRSEEAA
                     RQADRRTEDFFERHLG"
     gene            1252..1716
                     /locus_tag="VGB57_00145"
     CDS             1252..1716
                     /locus_tag="VGB57_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129784.1"
                     /translation="MNEVAPVQALAPNNWLVAGGILSALAAILHLAIIAGGPDWYRFF
                     GAGETMARMAERGLLRPTFYAIAIASILAIWSAYAFSGAGLLPRLPLIRTALVAISAA
                     YLLRGLAPIPLALLRPDLLGPFALWSSLIVLVYGICYAAGTWRAWPILSAGS"
     gene            1718..2698
                     /locus_tag="VGB57_00150"
     CDS             1718..2698
                     /locus_tag="VGB57_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY0129785.1"
                     /translation="MNDAYAFTLTRTGGPEVLEAEHIDVPRPGPGEVLVRNEAVGLNF
                     IDTYHRSGLYKLPLPSGLGGEGAGVVEAVGEGVTGFREGHRVGYFTGPLGSYATHRTI
                     AADKLIRLPDSIGFESAAAAMLKGCTAEYLIERCARVEAGQTVLVHAAAGGMGSILVQ
                     WLKAVGATVIAHAGDSRKAALAAELGADHALCCPMDALAAEVRALTGGQGVPVVLDGV
                     GEASWKASLGSVARRGLLVTYGNASGAVPAFTALDLLSAGSIFVTRPTLADYCRSPDE
                     MAASAARLFEMIGKGAVTIRIGATFPLLKAADAHRAIEARQTTGSTVLLP"
     gene            complement(2699..2950)
                     /locus_tag="VGB57_00155"
                     /pseudo
     CDS             complement(2699..2950)
                     /locus_tag="VGB57_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            complement(3390..>3508)
                     /locus_tag="VGB57_00160"
     CDS             complement(3390..>3508)
                     /locus_tag="VGB57_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0129786.1"
                     /translation="AKYIERYRWIAWIGLIIIVYVAGKMILEGWHDVQPMIG"
BASE COUNT          575 a         1189 c         1169 g          575 t
ORIGIN      
        1 tgtcgcgagg gtcgcgaccc tgggcgcggg aagcctgccg gcgagcgcct cgagcgccga
       61 gtgcgcgcga gtcccgctga tccgaaccac ggccaaggcc gcgggcgggc tgccggacga
      121 caaggcgaag atggtctcga tcacgctatt ctctcgcgac taaccgtcat cccggcgaaa
      181 gccgggacct cgggacgaga agtctcggcg ggtctcccag aggtcccggc ttccgccggg
      241 acgacggctc aacccttctt cccgcccatc ccctcgagca tcgagcgcca catcgtcatc
      301 ccggcctcgc tcatcgggcc ccaggccttc atcatctccg cggccgattc ggccgtgagg
      361 ccttcgctcg ccgccctggt catgcgctgg acataggctt cgtggaccgg agtcaggtcc
      421 gggaggccga ggaagcgccg cgcctcctcc ggagtgcagt cgacgtcgac gctgatcttc
      481 atggccggtc cttccgaaag ctccatcgct atgtgtatag aagcgccgcc cgagacaagg
      541 aggagccgac atggggacga tccggatcga cacgctggaa ggggaaggcg gcttcgacgc
      601 ctatgccgcc gaacccgagg gaggcgccgg cccgcgcgcg gcgatcgtcg tcatccagga
      661 gatattcggg atcaacgagg gcatcaggcg caaatgcgac gcctgggcgg acaagggcta
      721 tctcgctttg gcgccggatc tcttctggag gctcgagccg ggcgtcgagc tcgatcccga
      781 cgtgccggac gagttcaggc aggcgctcga cctgatgggc cggttcgacc aggatcaggg
      841 ggtcgtggac atcgaggcga cgattcgcgc cgcccgcggc cggctggatg agggcgcaaa
      901 ggtcggaatc gtcggctact gcctcggcgg ccggcttgcc tacatggcgg ccgcgcgcac
      961 cgacgccgat gcgagcgtgg gctattatgc ggtcggcctg cccaacatcc tccacgagaa
     1021 gaacgcgatc gcccggccgc tgatgctcca catcgcgggc gccgaccatt tcgtcgacga
     1081 ggcggcgcag cgagcgatgc acgaggcgct cgatgatcat ccccgggtca ccctccacga
     1141 ttatcccggc gaagaccacg gcttcgccgc cgagatgggc aagcggcgga gcgaggaagc
     1201 ggcacggcag gcggaccgcc gcaccgaaga cttcttcgag cggcacctcg ggtgaatgaa
     1261 gtcgcgccgg tgcaggcgct ggcgcccaat aactggctgg tcgcgggcgg aatcctgagc
     1321 gccctcgccg cgatcctcca cctggcgatc atcgccggcg gcccggactg gtaccgcttc
     1381 ttcggcgccg gcgagacgat ggctcggatg gcggaacgag gcctgcttcg ccccaccttc
     1441 tacgcgatcg ccatcgcctc gattctcgcg atctggtccg cttatgcctt ttccggcgca
     1501 ggcctcctcc cccgcctgcc gctgatccga accgcgctgg tcgcgatcag cgcggcctac
     1561 ctgctgcgcg gcctcgcccc gatcccgctc gccttgctcc gtcccgacct cctcggcccg
     1621 ttcgcgctgt ggagctcgct gatcgtcctc gtctacggaa tctgctacgc cgccggcacc
     1681 tggcgcgcct ggcccatcct ctccgccggg agctgaaatg aacgacgctt acgccttcac
     1741 actcacccgc accggcggcc ccgaagtgct cgaggccgag catatcgacg tcccgcgtcc
     1801 cggcccgggc gaggttctgg tccgcaacga ggcggtgggg ctgaacttca tcgacaccta
     1861 tcatcgctcg ggcctctaca agctgcctct gccctcgggg ctgggcggcg agggcgccgg
     1921 ggtcgtcgag gcggtcgggg agggggtgac cggctttcgc gaaggccatc gggtcggcta
     1981 tttcaccggg ccgctcggat cctacgccac ccaccggacg atcgccgccg acaagctgat
     2041 caggctgccc gactcgatag gcttcgagag cgcggcggcg gcgatgctca agggctgtac
     2101 cgccgaatat ctgatcgagc gctgcgcccg ggtggaagcg gggcagacgg tcctcgtcca
     2161 cgccgcggcg ggcggcatgg gctcgatcct cgtgcaatgg ctgaaagccg tcggagcgac
     2221 ggtgatcgcg cacgccgggg acagccgcaa ggcggccctg gcggcggagc tcggcgccga
     2281 ccatgccctg tgctgcccga tggacgcgct ggcggcggag gtgcgggcgc tgaccggcgg
     2341 ccagggcgtt ccggtggtgc tcgacggcgt gggggaggcg agctggaagg cgtcgctcgg
     2401 ctcggtcgcc cgtcgcggcc tgctcgtcac ctacggcaac gcctccggcg cggtgcccgc
     2461 cttcaccgcg ctcgacctgc tcagcgccgg ctcgatcttc gtcacccggc ccaccctcgc
     2521 cgattattgc aggagcccgg acgagatggc ggcttcggcg gcgcggctgt tcgagatgat
     2581 cgggaaaggc gcggtgacga tccggatcgg cgccaccttc cccttactca aggccgccga
     2641 cgcccaccgg gccatcgagg cgcggcagac gacggggtcg accgtcctcc ttccctgatc
     2701 aggtaaagag cgttcccagc ccaacggcgg ggtcgacgaa gccttcgtag accatcttgc
     2761 cggcgacata gacgatgacc agcagtccga cccacgcgat ccagcggtag cgcacgatat
     2821 atttggcgag gatgttggcg gcgacgccca tcagggcgac ggcgagcaac aggccgatga
     2881 tgaggatgcc cggatgctcg cgcgccgctc cggccaccgc cagcacgttg tcgaggctca
     2941 tcgagacgtc gacgccgcat ccgaaatggt tgcaactgca cgagcatttc cagtgacgac
     3001 ggtctatttc gaaaaggcgt cacgaaacaa ataaacgacc acgccaaaaa gggagaagat
     3061 aatgagagag tccaccaaag ctgtacttgc agccctgact ttggccgccg tgtcggtacc
     3121 tgcggtcgct cttgcgcagg caggcccagg tgacactgtt tgtagtgttg aatgtggagc
     3181 cagcgcaacc tgcgccattg tgcaatggtt gttttgctaa gatagttggc gggagggcac
     3241 tcgtgtcctc ccgcctcact ctaggtccgt cgttccggtg gctgaagcaa cgttgaccga
     3301 tgtcttccta cttcatgcgt tctacgaacc gagcgcataa atccaagggc tcgagacgcg
     3361 gtctacgacc gggtcgacga tgctgtcccc tagccgatca tcggctggac gtcgtgccag
     3421 ccttccagga tcatcttgcc ggcgacgtag acgatgatga tcagtccgat ccaggcgatc
     3481 cagcggtagc gctcgatata tttggcga
//
LOCUS       DASWNU010000008         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_493172, whole genome shotgun sequence.
ACCESSION   DASWNU010000008 DASWNU010000000
VERSION     DASWNU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493172"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1228
                     /locus_tag="VGB57_00165"
     CDS             44..1228
                     /locus_tag="VGB57_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0129787.1"
                     /translation="MSDLESFRLETRAWLEANCPAEMREPVRDESDACWGGRNFEFKN
                     EAQKRWMEVMAERGWTVPDWPAEYGGGGLSPAETKILKQEMARIGARNPLMSFGISML
                     GPALLKYGTEEQKKRFLTEIARGEIRWCQGYSEPNAGSDLASLQTKCEDKGDHWLVNG
                     QKVWTSYADKADWIFCLVRTDFQAPKHKGISFILFDMASPGVSTRPILLISGSSPFCE
                     TFFDDVKVPKEQLVGELNKGWDVAKYLLGHEREMISGMGLGGQTGSLGEALVESIGRD
                     GDGRLADPVLRADIARFDVDAMAFRAMSERFIDELKVGMAHPAQPSMMKYAGTELNKA
                     RHELVMAAGGSNALEWESERSRKGKSAREWLRTKANSIEGGTSEVQLGIIAKRILDLP
                     GA"
     gene            1336..2451
                     /locus_tag="VGB57_00170"
     CDS             1336..2451
                     /locus_tag="VGB57_00170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0129788.1"
                     /translation="MPLYLTEDQVMLRDTARQLMADEGSIAGQLRKYRDMGCKDGFGH
                     ALWKQLAELGFTGILIDEADGGLGLGHVEAGIVLEEIGRNLTPSPFLISAVAVVEALK
                     DTPHAQRWFPGILSGDTVAALAVDEGPKHRPEAIAMRGERAGNGFRLLGRKQFVVQGA
                     SADLIVVAARTDEGVTLFAVEKDSKGLEIEGVRLADSSIGARLDFQGVEVNADAVIGE
                     VGGGEAVLGRVLDAGRAGAASEMVGLGSAAMGMTVDYLKQRKQFGRLIGEFQVLQHRA
                     AHLYSELEIARSAALKAQQLLDEGSPEAEAMVSVAKAKAGRASTLAVQEGVQMHGGIG
                     MTDEYDIGLYMKRDRALNELFGDSNYHADKLARMKGY"
     gene            2486..3628
                     /locus_tag="VGB57_00175"
     CDS             2486..3628
                     /locus_tag="VGB57_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0129789.1"
                     /translation="MPLDPESFELLLSTVRRFVRERLRPLEERVDREDEVPPQIVAEM
                     REMGLFGLSIPEEYGGLGLTMSEEVRVALELGGTTPAFRSTFGTNVGIGSQGLVMAGS
                     EAQKAEWLPRIATGEIVTSFALTEPGAGSDSASVQTKAVRDGDVYRLTGTKRFITNAD
                     KASLFTVMARTGEPGAKGVSAFLVPADLPGVSVGRPEKKMGQQGAHVCDVNFDEAPVP
                     AANRLGEEGEGFRIAMRVLDRGRLHIAAVCVGIAERLIADAVGYAAERKQFGKPISEF
                     QLIQAMIADSKTETMAAKALVLEAAALKDKGEPITLEAAAAKYFASETVGRVADRAVQ
                     IFGGAGYIADYGIERLYRDVRLFRIYEGTSQIQQLVIAREVMKRGG"
     gene            complement(3723..4034)
                     /locus_tag="VGB57_00180"
     CDS             complement(3723..4034)
                     /locus_tag="VGB57_00180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0129790.1"
                     /translation="MLWVMPFREEVVGRPGFLHGGAIAGLLEFAALGTLFEALGEGAT
                     ARPINVSVDFMRGGTDHDTFASAVVTRLGSRVANVEAHAWQQDRGKPIAAARMNLMVR
                     R"
BASE COUNT          729 a         1289 c         1441 g          609 t
ORIGIN      
        1 gcccgtgccg ccaccgatgg cccataaagg agagcgcgac ccgatgagcg accttgaaag
       61 cttccgtctc gaaacccgcg cctggctgga ggcgaactgc ccggccgaga tgcgcgagcc
      121 ggtgcgcgac gaaagcgacg cctgctgggg cgggcgcaac ttcgagttca agaacgaggc
      181 ccagaaacgc tggatggagg tgatggcgga gcgcggctgg accgtgcccg actggccggc
      241 cgaatatggc ggcggcggcc tttcgccggc cgagacgaag atattgaagc aggagatggc
      301 gcggatcggc gcgcgcaatc cgctgatgag cttcggtatc tcgatgcttg gtcctgcgct
      361 gctgaaatat ggcacggagg agcagaagaa gcgcttcctg accgagatcg cccgcggcga
      421 gatccgctgg tgccagggtt attcggagcc gaacgccggc tccgacctcg cctcgcttca
      481 gacgaagtgc gaggacaagg gggatcattg gctggtcaac ggccagaagg tctggaccag
      541 ctacgcggac aaggccgact ggatcttctg cctggtgcgc accgatttcc aggcgcccaa
      601 gcacaagggg atcagcttca tcctgttcga catggccagc ccgggcgtgt cgaccaggcc
      661 gatcctgctc atttccggct cctcgccttt ctgcgagacc ttcttcgacg acgtgaaggt
      721 gcccaaggag caactggtgg gtgagctcaa caagggctgg gacgtcgcca aatatctgct
      781 cggccacgag cgcgagatga tcagcgggat gggcctgggc ggccagaccg gaagcctcgg
      841 cgaggccctg gtcgagagca tcggccgcga cggggacgga cggctggccg atccggtgct
      901 ccgcgccgac atcgcccgct tcgacgtcga cgccatggcc ttccgggcga tgtccgaacg
      961 gttcatcgac gagctgaagg tggggatggc gcatccggcc cagccctcga tgatgaaata
     1021 tgcgggcacc gagctcaaca aagcgcggca cgagctggtc atggccgcgg gcggctcgaa
     1081 cgcgctggaa tgggagagcg agaggagccg caagggcaag tcggcgcgcg agtggctgag
     1141 gaccaaggcc aattcgatcg aaggcgggac cagcgaagtg cagttgggga tcatcgcgaa
     1201 acggattttg gatttgccgg gagcataaga gaagaagctg gattgcttcg tcgcttcgct
     1261 cctcgcaatg acggggatgc aagccgtcat tgcgagcgga gcgaagcaat ccagctctgc
     1321 ctaggagaca aatacgtgcc gctctacctt accgaagacc aggtcatgct tcgcgatacg
     1381 gcgcggcagc ttatggcgga cgaagggtcg atcgccggcc agcttcgcaa atatcgcgac
     1441 atgggttgca aggacggctt cgggcacgct ttgtggaagc agctcgccga gttgggcttc
     1501 accgggatcc tgatcgacga ggccgacggc ggtctcggcc tcggccacgt cgaggccgga
     1561 atcgtgctcg aggagatcgg ccgcaacctg actccttcgc ccttcctgat cagcgctgtc
     1621 gccgtcgtcg aggcgctgaa ggacacgccg cacgcgcagc gctggtttcc cgggatactc
     1681 tccggagaca cggtggcggc gctcgccgtc gacgagggcc ccaagcacag acccgaggcg
     1741 atcgcgatgc ggggcgagcg ggccggcaac ggcttccggc tcttggggcg caagcagttc
     1801 gtcgtgcagg gcgcttcggc ggacctgatc gtggtggcgg cgcggacgga cgagggcgtg
     1861 accctgttcg ccgtggagaa ggattcgaag gggctggaga tcgaaggcgt ccggctggcc
     1921 gattccagca tcggcgcccg gctcgatttc cagggcgtcg aggtcaatgc cgacgccgtc
     1981 atcggcgagg tcggcggcgg cgaggcggtg ctcggccggg tcctcgatgc cggccgcgcc
     2041 ggcgccgcct cggagatggt cgggctcggc tcggcggcga tggggatgac ggtcgactat
     2101 ctcaagcaaa ggaagcagtt cggccggctg atcggcgagt tccaggtgct ccagcaccgc
     2161 gccgcgcacc tttattcgga gctggagatc gcgcgctcgg cggcgctcaa ggcgcagcaa
     2221 ttgctcgacg agggcagccc ggaagcggaa gcgatggtct ccgtcgccaa ggccaaggcc
     2281 ggacgggcct cgacgctcgc ggtgcaggaa ggagtccaga tgcacggcgg catcggaatg
     2341 accgacgaat atgacatcgg cctctacatg aagcgtgacc gggccctgaa cgagctgttc
     2401 ggggattcga actaccacgc cgacaagctg gcgcggatga aggggtatta gctccccggt
     2461 cttcgccggg gaacaaggag aaccaatgcc cctcgatccc gaatccttcg agcttctcct
     2521 ctccacggtc cgccgcttcg tccgcgagcg cttgcgcccg ctcgaggaac gcgtcgaccg
     2581 cgaggacgag gtgcctccgc agatcgtcgc cgaaatgcgg gagatggggc tgttcggcct
     2641 ctccatcccc gaggaatatg gcgggctcgg ccttaccatg tcggaggagg ttcgggtcgc
     2701 gctcgagctc ggcgggacga cccccgcctt ccgctccacc ttcgggacca atgtcggaat
     2761 cggcagccag ggcctggtca tggcggggag cgaggctcaa aaggccgagt ggctgccgcg
     2821 catcgcgacc ggcgagatcg tcaccagctt cgccctcacc gaacccggcg ccggctccga
     2881 cagcgcgtcg gtgcagacga aggcggtgcg cgacggcgac gtctaccgcc tgaccggcac
     2941 caagcgcttc atcaccaatg ccgacaaggc ctcgctcttc accgtcatgg cccgcacggg
     3001 ggagcccggg gccaagggcg tctccgcctt cctcgttccc gccgacctgc ccggggtaag
     3061 cgtcggcagg ccggagaaga agatgggcca gcagggcgcc catgtctgcg acgtcaattt
     3121 cgacgaggcg ccggtgcccg ccgccaaccg gctcggcgag gaaggcgagg ggttccggat
     3181 cgcgatgcgg gtgctcgacc gcggccggct ccacatcgcc gcggtctgcg tcgggatcgc
     3241 cgagcgcctg atcgccgacg ccgtcggcta tgcggcggag cgcaagcagt tcggaaagcc
     3301 catatccgag ttccagctca tccaggcgat gatcgccgat tccaagacgg agacgatggc
     3361 cgcgaaggcg ctggtgctgg aagcggcggc gctgaaggac aaaggcgagc cgatcacgct
     3421 cgaggcggcg gccgccaaat atttcgcgag cgagaccgtg gggcgggtcg ccgaccgggc
     3481 ggtgcagata ttcggcggtg ccggctacat cgccgattac ggcatcgagc ggctgtaccg
     3541 ggacgtccgc ctgttccgca tctacgaggg cacgagccag attcagcagc tggtgatcgc
     3601 aagggaagtg atgaaaaggg gcgggtgagc gcgtcgtccc agcgaaggct gggaccccag
     3661 cgggtgaaga acgcggcgcc tttcaccgcc cggggcccca gccttcgctg gggcgacggc
     3721 tatcagcgcc tcaccatcaa attcatccga gccgccgcga tcggcttccc ccggtcctgc
     3781 tgccaggcat gagcctcgac gttagccacc cggctgccga gccgggtcac caccgccgag
     3841 gcgaaggtgt cgtgatcggt gccgccgcgc atgaagtcga cgctgacgtt gatcggcctg
     3901 gcggtcgcgc cctcgcccag cgcctcgaag agggtaccca gcgccgcgaa ttcgagaagg
     3961 ccggcgatgg cgccgccgtg aaggaagccg ggacgtccga ccacctcctc gcggaacggc
     4021 atcacccaga gcagggcgcc gtccccggag cgctcggtgc gaagcccg
//
LOCUS       DASWNU010000009         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_493261, whole genome shotgun sequence.
ACCESSION   DASWNU010000009 DASWNU010000000
VERSION     DASWNU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493261"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..>1703
                     /gene="alaS"
                     /locus_tag="VGB57_00185"
     CDS             65..>1703
                     /gene="alaS"
                     /locus_tag="VGB57_00185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0129791.1"
                     /translation="MTSTNDIRRAFLDHFGREGHQIVPSAPLVPQNDPTLMFVNAGMV
                     PFKNVFTGLESRPYRTATSSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KERAIALAWDLLTREFGVSKDRLTATVYHTDDEAYDLWKRIAGLPEERIIRIATKDNF
                     WAMGDSGPCGPCSEIFFDHGDHIPGGPPGSPDEDGDRFTEIWNLVFMQYEQEDNEIVR
                     DLPTPSIDTGMGLERIAAVLQGVHDNYDTDTFKALIAASGELTRTETGGTNKASHRVI
                     ADHLRASGFLIADGVLPANEGRGYVLRRIMRRAMRHAHLLGAAEPLMHRLVPELVSEM
                     GGPYPELVRAQPLIEETLRSEETRFRQTLANGLRLLDEATSGMGEGDTLPGTVAFKLY
                     DTFGFPYDLTEDALRAQGLAVDRAGFDAAMAEQKAAARAAWKGSGEKASDDIWFDIAE
                     AQGSTEFTGYAGHQGEGVVVGLVKDGKPVGQAGEGDSVILLTNQTPFYGESGGQMGDA
                     GKIGNDNGFAADVSDTSKPLGRLHAHHALVKSGSVRLG"
BASE COUNT          302 a          582 c          553 g          266 t
ORIGIN      
        1 gggccttcgc ctcgccgctg aggctttcct tgagcggggg atgcggctcg cctaagtcaa
       61 ccttatgact tccacgaacg acattcgccg cgccttcctc gaccatttcg gccgtgaggg
      121 gcaccaaatc gtgccctcgg cgccgctggt gccgcagaac gaccccactt tgatgttcgt
      181 caatgcgggg atggtgccgt tcaagaacgt cttcaccggc ctcgaaagcc ggccgtaccg
      241 caccgccacc tcctcgcaga aatgcgtgcg cgccggcggc aagcataacg acctcgacaa
      301 tgtcggctat accgcgcgcc accacacctt tttcgagatg ctggggaatt tctccttcgg
      361 cgattatttc aaggagcgcg cgatcgctct cgcctgggac ctgctgaccc gcgagttcgg
      421 cgtctccaag gatcgcctga ccgccaccgt ctaccacacc gacgacgagg cctatgatct
      481 ttggaagagg atcgccggcc tgcccgagga gcggatcatc cgcatcgcca ccaaggacaa
      541 tttctgggcg atgggcgaca gcggcccatg cgggccctgc tccgaaatct tcttcgacca
      601 tggcgatcac atccccggcg ggccgcccgg cagccccgat gaggatggcg accgcttcac
      661 cgagatctgg aacctcgtct tcatgcagta cgagcaggag gacaatgaga tcgtccgcga
      721 cctgccgacg ccgagcatcg acaccggcat ggggctcgag cggatcgccg ccgtgctcca
      781 gggcgtccac gacaattacg acacggacac gttcaaggcg ctgatcgccg cctccggcga
      841 actcaccagg acggagacgg gcggaacgaa taaggccagc caccgggtca tcgccgacca
      901 tctgcgcgct tcgggcttcc tgatcgcgga cggcgtgctg ccggccaacg agggcagggg
      961 ctatgtcttg aggcgcatca tgcgccgggc gatgcgccac gcccatctgc tcggggcggc
     1021 cgagccgctg atgcaccggc tcgtgccgga gctggtctcg gagatgggcg gcccctatcc
     1081 ggaactggtc cgcgcccagc cgctgatcga ggagacattg cgctccgaag agacccgctt
     1141 ccggcagacc ctcgccaacg gcctgcgcct gctcgacgaa gccacttccg gcatgggcga
     1201 gggcgacacg ctgccgggca cggtcgcgtt caagctctac gacacgttcg gcttccccta
     1261 cgacctcacc gaggatgcgc tgcgtgccca gggcctggcg gtcgaccgcg ccggcttcga
     1321 cgccgcgatg gccgagcaga aggccgcggc ccgcgccgcc tggaagggct cgggcgaaaa
     1381 ggcgtccgac gacatctggt tcgacatcgc cgaggcgcag gggagcaccg agttcaccgg
     1441 ctatgccggc catcaaggcg agggggtcgt ggtcgggctg gtgaaggacg ggaagccggt
     1501 cggccaggcg ggcgagggcg acagcgtcat cctgctgacc aaccagaccc ccttttacgg
     1561 cgagtccggc gggcaaatgg gcgatgccgg aaagatcggc aacgacaacg ggttcgctgc
     1621 ggatgttagc gacacgtcaa agccgctcgg ccgcctccac gcccatcacg cgctggtgaa
     1681 atcgggctcc gtgaggctcg gcg
//
LOCUS       DASWNU010000010         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_363434, whole genome shotgun sequence.
ACCESSION   DASWNU010000010 DASWNU010000000
VERSION     DASWNU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363434"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..953)
                     /locus_tag="VGB57_00190"
     CDS             complement(156..953)
                     /locus_tag="VGB57_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0129792.1"
                     /translation="MSRSQGLVRVGVGGWDYDPWRETFYPRGLPKAKQLEHMAARLTA
                     VEINATYYKLQKPELFERWAKAVPDGFRFAIKGSRFCSNRKVLGEAGEAVGRFCGQGL
                     AELGDRLGPVLWQFMATKRFEPDDFRAFLELLPRKVAGVPLRHVVEPRHESFRDPAFV
                     AMARAAKVAIAFADSDEFPCIPDLSGELVYARLQRSREAAATGYPAAELERWAEVAKR
                     WARGESPEGLAYVARPARPGAREVFVFFTSGAKARNPAAAEALIGRL"
     gene            1112..1336
                     /locus_tag="VGB57_00195"
     CDS             1112..1336
                     /locus_tag="VGB57_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129793.1"
                     /translation="MTTVTAFENQCRAHGEAAKLPFAASRGAIGAILFLLVAFFLLAA
                     GAVTGAPQGSGAPVEANAFILDPLVGSAQA"
     gene            1357..2379
                     /locus_tag="VGB57_00200"
     CDS             1357..2379
                     /locus_tag="VGB57_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0129794.1"
                     /translation="MKRLGLLAFILIAGCGSAPQPVAGGPRLRAEPAAGFRDDEGPGR
                     EAAFSPDGRLLATTSASGLVTLRRMPGLKVVHRFRHPGGAAAIVFSPDGKGLATAGYD
                     GVVRLWETAGGRPGGALEEARGTVWSLDYSPDGSRLAAAGEDGVVRIWNSEGRVAARL
                     QGHGRNVWEVRFSPDGRRLASGSFDATARLWDSATGKALATLREHEEAVVGLAWSGDG
                     RWLATGGDDSTILIRRSTDGRPVRRLEAGNHVYKIAFSPDSRWLATAGRARGGLGTLW
                     HGLTGAGGDGEAVRLWRVADGALIQALKMPEDVMYVGFSPDGRRLVASGDDGTVTLWT
                     LRAGTE"
     gene            2414..2605
                     /locus_tag="VGB57_00205"
     CDS             2414..2605
                     /locus_tag="VGB57_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129795.1"
                     /translation="MQDMIDDGLAIILACLLIAGLASALIVVRPWRRRLRHRRRHSHR
                     TKIDLFAVEAGEPAAKTDA"
     gene            2655..3659
                     /gene="argC"
                     /locus_tag="VGB57_00210"
     CDS             2655..3659
                     /gene="argC"
                     /locus_tag="VGB57_00210"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0129796.1"
                     /translation="MIRVAILGASGFVGAELLRLCAQHPRLRPVKLFGSSQAGQKLDS
                     VHPQLALAYPDVLVNSYAPDALDEGVDLVFAALPHGHSQAVARDVIARSIPFVDLGAD
                     FRLDSAADYQLWYREPHGAADLIGRFVYGIPELHRGSISGSTTVAAAGCYATAAILAM
                     KPLMALVEPGSLVVDAASGVSGAGKGLKEETHFNSVDESFAAYGLLAHRHTAEMEMAL
                     GASLLFTPHLVPMNRGILATCHAVAKGPCDPLAALRDAYAGEPFIHVSERPPATKWTL
                     GSNAVHLTARYDERTGRVLAIAALDNLVKGAAGQMIQCANLMLGLEETAGLPVAGVYP
                     "
BASE COUNT          572 a         1376 c         1267 g          587 t
ORIGIN      
        1 gcgggttcat tgcgcccgta ggccgcccca tgcgcagcat ggggaggggg accatgcgaa
       61 gcatggtgga ggggtgactt ggatagacct cgtcagatca cccctccacc gccttcggcg
      121 gtccccctcc ccatcgtttc acgacgggga ggcagctaca gccgcccgat cagcgcttcc
      181 gcggccgccg ggttgcgcgc cttggcgccc gaggtgaaga agacgaagac ctcccgagcc
      241 cccggcctgg ctggcctggc gacataggcg aggccttccg ggctttcgcc ccgcgcccac
      301 cgcttcgcca cctcggccca gcgctccagc tccgccgccg gatagcccgt cgccgccgcc
      361 tcccggctgc gctggagccg cgcatagacc agctcgccgg acaagtcggg gatgcacggg
      421 aattcgtcgg aatcggcgaa ggcgatcgcc accttcgccg cccgcgccat cgccacgaag
      481 gcgggatccc ggaagctttc gtgcctcggc tcgaccacgt ggcgaagcgg cacgcctgcg
      541 accttgcgcg gcagaagctc caggaaggcc cggaagtcgt cgggctcgaa cctctttgtc
      601 gccatgaact gccacaggac ggggccgagc ctgtcgccca gctccgccaa cccctggccg
      661 caaaagcgcc ccacggcctc cccggcctcg ccgagcacct tgcggttcga gcagaagcgc
      721 gagcccttga tggcgaagcg gaagccgtcc gggaccgcct tcgcccagcg ctcgaacagc
      781 tcgggcttct gcagcttgta ataggtcgcg ttgatctcga ccgccgtcag cctcgccgcc
      841 atatgctcga gctgcttcgc cttcggcaat ccgcgcggat agaaagtctc gcgccacgga
      901 tcgtaatccc agccgccgac tccgactcgg acgaggccct ggctacgcga catggcatcg
      961 gcactccgca catctccgca catatccgta cgccccgggc aaggccagcc gcccgtccac
     1021 cgccgatgtg ccgaaacttg gctttactgc tggtggcggc gcgacggcgc gcggtcctaa
     1081 tctgcaggca cttccagcgg ggagcgggac aatgacgacg gtcacggctt tcgaaaatca
     1141 gtgcagggcc catggcgaag cggcgaagct gcccttcgcc gcttcgcgcg gcgcgatcgg
     1201 cgcgatcctg ttcctgctgg tggctttctt tctgcttgcc gcgggggcgg taaccggcgc
     1261 gccgcaaggg agcggcgcgc cggtcgaggc caacgcgttc atcctcgatc cgctggtcgg
     1321 ctcggcgcaa gcctgacctt ctgctagaag aggcgcgtga agcgcctcgg cctcctcgcc
     1381 ttcatcctga tcgccggctg cggcagcgct ccgcagcccg ttgccggcgg accgcgcctg
     1441 cgggcggagc cggccgccgg cttccgggac gacgaggggc ccggccggga ggccgccttc
     1501 agccccgacg gccgcttgct ggcgacgacc agcgcttccg gcctggtgac cctccgccgg
     1561 atgcccggcc tgaaggtcgt ccaccggttt cgccatccgg gcggcgccgc cgcgatcgtc
     1621 ttctcgccgg acggcaaagg gcttgccacc gccggctatg acggcgtggt ccggttgtgg
     1681 gagacggccg gcggccggcc cggcggcgcg ctcgaggagg cgcgcggcac cgtgtggagc
     1741 ctcgattatt cgcccgacgg cagccggctt gccgcggcgg gcgaggacgg cgtcgttcgc
     1801 atctggaaca gcgaagggcg ggtcgccgcc aggctgcagg ggcatggccg caacgtctgg
     1861 gaggtgcgct tcagccccga cggccgccgc ctcgccagcg gcagcttcga cgcgaccgcc
     1921 cggctgtggg attcggccac tggcaaggcg ctggcgaccc tgcgcgagca tgaggaagcg
     1981 gtggtcggcc tcgcctggag cggcgacgga cgctggctcg ccaccggcgg cgacgattcg
     2041 accatcctca tccgccgctc cacggacggc cggccggtgc ggcggctcga ggccggcaac
     2101 catgtctaca aaatcgcctt cagccccgac agccgctggc tcgccaccgc cggccgggcc
     2161 cgaggcggac tcggcacctt gtggcacggc ctgaccggag ccggcggcga cggggaggcc
     2221 gtccggctct ggcgggtggc ggacggagct ttgatccagg ccctgaagat gcccgaagac
     2281 gtgatgtatg tcggcttcag cccggacggg cgccggctgg tcgcgtccgg cgacgacggc
     2341 accgtcacct tgtggacgct tcgggctgga acggagtgac gacttgatct agattgcccc
     2401 cgggaggcga gccatgcagg acatgatcga cgacggcctg gcgatcatac tcgcctgcct
     2461 cctcatcgcc ggcctcgcca gcgctctcat cgtcgtccga ccctggaggc gccgcctccg
     2521 ccaccgcagg cgccactccc accggacgaa gatcgacctg ttcgcggtcg aagccggcga
     2581 gcccgccgcg aagacggatg cataaaacgc aggttattgc ataattatgc aatagggcgc
     2641 attggccgct ccccatgatt cgagttgcca tattgggagc ctcgggcttc gtcggagcag
     2701 agctgctgag actgtgcgct cagcatcccc ggttgcgccc ggtgaaattg ttcggaagca
     2761 gccaggcggg gcagaagctg gactcggtcc acccccagct ggctttggcc tatcccgatg
     2821 tcctcgtgaa tagttatgca ccggatgcgc tcgacgaggg cgtcgatctg gtcttcgcgg
     2881 cgctgcccca cggccattcg caggccgtcg cccgcgacgt catcgcccgc tccatcccgt
     2941 tcgtcgatct cggcgccgac ttccggctcg acagcgccgc ggactatcag ctctggtacc
     3001 gggagccgca cggcgccgcc gacctcatcg gccggttcgt ctacggtatt cccgagcttc
     3061 accgcggttc tatatcgggt tcgaccacgg tcgccgccgc cggctgctac gccaccgcgg
     3121 cgatcctcgc catgaagccg ctcatggcgc tggtcgagcc cggcagcctc gtcgtcgacg
     3181 cggcctcggg ggtcagcgga gccggcaagg ggctcaagga agagactcat ttcaacagcg
     3241 tcgacgagag cttcgccgcc tacgggcttc tcgcccaccg ccacaccgcg gagatggaga
     3301 tggcgctggg cgcaagccta ttgttcacgc cccatctcgt gccgatgaac cgcggtatcc
     3361 tcgccacctg ccacgccgtc gccaagggac cgtgcgaccc cctcgccgcg cttcgcgacg
     3421 cttatgccgg cgagcccttc atccacgtct cggagcgccc gccggcgacg aaatggaccc
     3481 tgggctcaaa cgccgtccac ctcaccgccc gctacgacga gcgcaccggc cgggtgcttg
     3541 cgatcgcggc gctcgacaac ctcgtcaaag gggccgccgg ccagatgatc cagtgcgcga
     3601 acctgatgct cggcctcgaa gagacggccg gattgccggt ggcgggagtc tatccatgag
     3661 cgggaaatgc caagatcctc ccctgcattt gcaggggagg gggaccgccc gaagggcggt
     3721 ggaggggccc ttcggcagca agcccctcca ccatgcttcg catggtcccc ctcccctcga
     3781 aatcgagggg aggattttca ga
//
LOCUS       DASWNU010000011         4122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_285803, whole genome shotgun sequence.
ACCESSION   DASWNU010000011 DASWNU010000000
VERSION     DASWNU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285803"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VGB57_00215"
     CDS             complement(<1..2079)
                     /locus_tag="VGB57_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129797.1"
                     /translation="MRRAAFAALLLLAPAVIWSQSTVPKHLQRDFGNDKGDESAPPSS
                     IDTPVVRINVVPESPSPSARETIVVAVVTATQDAGPVTIEVIAGGGVRAARPAGFASA
                     PAGTSYFRSMANRNRYIQRLPALNAGQSLRIRIPVTLSAVDGFVAVTVASPDGESFSE
                     NDTLFLGRGEGQTISSGSSLQDLRRQRLILRSRRQGSELVAPGTTLEEALIELEQEGR
                     VDMGGSSRPPIGPSVAPQAQISVTGRIQFTASDGDRHPVRGIRVEIRDEDTATADDIL
                     ATVQTNDQGEFSATVANTDEDGTGADLHAIAYAIGATVEVSRYAAINGVRPTRSIKSE
                     LRNNVGSGPIDLSIVASNDEANFPNAVAFEIYEAANYQSRYVRTLEGGTAPELVIIRY
                     PRLDEDGQDTSDSSWYGNGRIRLGLTDAHDWDNIMHEYGHHLQAKYQFGNNPGGSHTG
                     TQNHCQAQSSRDEGLRLAWSESWPTFFALQTQASLGLAGLGIPDLGDTLYQDRKPGTD
                     LVYNLEVPNILTSKPPSEGQEHALQRVTWDMFDTVNDISDANVAYPPARLWAAAKASG
                     TGRFASFWQAFSQPLSDGNRGPLGDILALHWIGPTTNPVSSPTGGGIPTLTWRVLLGC
                     SGGSQIRYRLRFWDPQTGANVLETPFQTGTQFTPSAAQIATLFPQGRTRVGWTVVSRD
                     QASPQTGDYPG"
     gene            complement(2459..2881)
                     /locus_tag="VGB57_00220"
     CDS             complement(2459..2881)
                     /locus_tag="VGB57_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129798.1"
                     /translation="MSRIRMEDVVLPPAPAHVLRRRRGIISELTIATALAGTIFAVAA
                     VLLLGGAVRALQVGQLFSALTLVLVLLAVYLTIGVVLCAAFVNRLRFWGGAPLSDIVE
                     AKANFVRRWLYSAPRLLTMLLFHRLTGTGAGSAAGPWT"
     gene            complement(2878..3654)
                     /locus_tag="VGB57_00225"
     CDS             complement(2878..3654)
                     /locus_tag="VGB57_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129799.1"
                     /translation="MRKASRRRGRAREAHLPRRQLAFVASVGLAVAAAGVSAFALRGP
                     VVDPETDCLAAAPPPASRVMAIDTTDRLVGPQMETVRAVSRRLIAELPEQGRLALVEI
                     RDGGPSELQPLLDRCVPRLNSNRARRRFEALVEEPLNEELGKLRRRPDAPQSPVMETT
                     FAIAAEPRWRDSAGRVTIVIATDAMQNTDALSFYRRGDRNPIVGRPLKKVSVILVPLR
                     NERDLARQGAAFDRLVEELQGAGAEVTYQRPPWLAFEEVR"
     gene            complement(3651..>4122)
                     /locus_tag="VGB57_00230"
     CDS             complement(3651..>4122)
                     /locus_tag="VGB57_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129800.1"
                     /translation="AVLITEAASTGRREADNVVRFAAEWAKTTKVEAQRAKGVIYQAN
                     RYLENLENAVLAVDSEYRAGNERMRTSSPPAWQPIQAFDGYFATPPEIEEAPKIAARN
                     SARWSEAARVETAAIGEAELKALAYIDGLVDLLPPNPPQPAAPAPALPWRAAPV"
BASE COUNT          700 a         1316 c         1428 g          678 t
ORIGIN      
        1 gccgggatag tcgccggtct gaggcgacgc ctggtcccgg ctcaccacgg tccagccaac
       61 gcgggtccgg ccctgaggaa aaagcgttgc gatctgggcg gccgaggggg tgaactgggt
      121 gcccgtctga aacggcgtct caaggacgtt ggcaccggtt tgcggatccc agaaacgcag
      181 ccgatagcga atctgcgaac cgccgctgca cccgagcagc actcgccagg tcagggtcgg
      241 gatgccgccg ccggtgggcg agctgaccgg gttggtcgtc ggtccaatcc agtggagcgc
      301 caatatatcg ccaagcgggc cccgattgcc gtccgacaaa ggctgcgaga aagcctgcca
      361 gaaggaggcg aagcggccgg tgccgctcgc cttggccgcg gcccagagcc gcgcgggcgg
      421 ataggcgaca ttcgcatcgg agatgtcatt gaccgtgtcg aacatgtccc aggtcacgcg
      481 ctggagggca tgttcctggc cttcgctcgg cggcttgctg gtgaggatgt tgggaacctc
      541 gagattgtag acgaggtccg tacccggctt ccggtcctga taaagcgtgt cgccgaggtc
      601 ggggataccg agaccggcaa ggccaaggct cgcctgggtt tgaagcgcaa agaaagtggg
      661 ccaggattcg gaccaggcca atctcaggcc ctcgtcgcgc gagctctgcg cctgacaatg
      721 attttgggtg ccggtatggg agccgcccgg attgttgccg aactgatatt tggcctgcag
      781 gtgatggcca tattcgtgca tgatattgtc ccaatcatgc gcgtccgtca ggccgagccg
      841 gatgcggcca tttccgtacc aggaagaatc ggacgtatcc tggccgtcct catcgagccg
      901 cggatagcgg atgatgacga gttcgggcgc cgtcccccct tcgagcgtcc ggacgtatcg
      961 ggactggtaa ttggctgcct cgtaaatctc gaacgcgacg gcattgggga aattggcctc
     1021 gtcgttcgag gcaacgatcg agagatcgat gggtcccgag ccgacattgt tgcgtagttc
     1081 cgatttgatg gagcgggttg gacggacacc gtttatggcc gcgtagcggc tgacctcgac
     1141 agtcgcgccg atcgcataag cgatcgcgtg cagatcggct ccggttccgt cctcgtcggt
     1201 gttggcgacc gtggcgctga actcgccctg atcgttggtc tggacggtgg cgaggatgtc
     1261 atcggccgtg gccgtgtcct catcgcgtat ctcgacgcgt atgccgcgca cggggtgccg
     1321 atcgccgtcg cttgcggtga actggatccg gccagtgacc gagatctgcg cctgcggagc
     1381 aacgctcgga cctatcgggg gccgcgaaga gccgcccata tcgacgcgcc cttcctgctc
     1441 gagctcgatc aacgcctctt caagcgtcgt tcccggagcc accaattccg atccttgccg
     1501 acgcgagcga aggatgaggc gctggcgccg cagatcttgc aggctgctac cgctggaaat
     1561 cgtctgaccc tctccccgac cgaggaacaa ggtatcgttt tcggagaagc tttcgccgtc
     1621 cggggaggcg acagtaacgg caacaaagcc gtcgacggcc gagagtgtta ccggaattcg
     1681 gatccgaagg ctttgccccg cattgagggc cggcaagcgc tggatgtacc ggttccggtt
     1741 cgccatgctc ctgaaatagg acgtgccggc cggggcggac gcgaaaccgg ccggacgcgc
     1801 ggcgcgaacg ccgcctccgg cgatgacctc gatcgttacc ggcccggcgt cctgggtggc
     1861 ggtgacgacc gcgaccacaa tcgtttctcg cgcggacggc gagggcgact ccggtacgac
     1921 gttgatgcga acgacgggcg tatctatcga agacggcggt gcagactcgt cacctttgtc
     1981 gttgccgaaa tctcgctgta gatgcttcgg aacggtcgat tgcgaccaga tcaccgcagg
     2041 cgccaaaaga agcagggcag caaaggcagc tcgccgcatg tcatcctcca cggaagcgag
     2101 gagtgtacac catcgtgcga gcaaatggca gagaccgttc aattacctat tcacaccttc
     2161 gtcgacgccg gcgcggaccc gcatctcaaa tcggtcttaa acagcatctg agatcatgcg
     2221 acccgcctcg ggacgggcgg tggccgatgg acgactattg cggggccccg tcgctcgcag
     2281 cgctttcagc gggatttgcc gtgagaccgc ctatcgaccg atgtcgcgct ggcgaagatg
     2341 cttcgcgccc accgcggcgt tcgccggccg gtcggggaac ggttggcggg cgcgccccta
     2401 ggcggccaac gacgatccta gcgagggtga cgcggcttgg aactctagcc ggaacgacct
     2461 aggtccaagg accagccgcc gaaccggccc cggtgccggt aaggcggtgg aacagcagca
     2521 tggtcagaag cctcggcgcg gagtacagcc accgccggac aaaattggcc ttcgcttcga
     2581 cgatgtcgct gaggggcgcc ccgccccaga agcggagcct gttcacgaac gccgcgcaga
     2641 gcacgacgcc gatcgtcagg tagacggcga ggagaacgag gacgagagtc agagccgaga
     2701 aaagctggcc gacctgaagg gctctgaccg cgccccccaa gaggagcacg gccgcgacgg
     2761 cgaagatggt gcccgcgagg gcggtcgcga tcgtcagctc gctgatgatc ccgcgccgac
     2821 gccgaaggac atgcgccggc gccggcggga ggacgacgtc ttccattcgg atccggctca
     2881 tctcacctcc tcgaaggcca gccagggggg ccgctggtag gtgacctcgg ccccggcacc
     2941 ctgaagctcc tccaccaagc ggtcgaaggc ggcaccctgg cgcgcgaggt cccgctcgtt
     3001 tctcagcggc accaggatga ccgacacttt cttcagcggc cggccgacga tcgggttgcg
     3061 gtcgccacgc cggtagaagg agagcgcatc ggtattttgc atggcgtcgg tcgcaatcac
     3121 gatggtgacc cgcccggcgc tgtcgcgcca cctcggctcc gcggcgatcg cgaacgtcgt
     3181 ctccatcacc ggcgactgcg gcgcgtcggg ccgcctccga agcttgccga gctcctcgtt
     3241 cagcggctcc tcgacgagag cctcgaagcg gcgccttgcg cggttgctgt tgagccgtgg
     3301 gacgcagcgg tcgagcagcg gctgcagctc cgacggcccg ccgtcccgga tctcgacgag
     3361 cgccagccgg ccctgctccg ggagctcggc gatgaggcgg cgcgagaccg cgcgcacggt
     3421 ttccatctga ggacccacga gacggtcggt cgtgtcgatc gccatgaccc ggctcgccgg
     3481 cggcggcgcc gccgcgaggc agtccgtttc gggatccacc accgggccgc ggagggcgaa
     3541 ggcgctgacg ccggctgcgg cgacggcgag gccgacgctg gcgacgaagg cgagttggcg
     3601 gcgcggaaga tgagcctcac gagcgcgtcc gcggcggcgg gatgccttcc tcataccggc
     3661 gcggccctcc agggcagggc gggcgcgggc gcggccggct ggggcggatt ggggggaagc
     3721 agatcgacga ggccgtcgat ataggccagc gccttgagct ccgcctcccc gatcgccgcc
     3781 gtctcgacgc gggcggcctc ggaccagcgg gcgctgttcc tcgccgcgat tttcggggcc
     3841 tcctcgatct ccggcggcgt ggcgaagtag ccgtcgaaag cctgtatggg ttgccacgcc
     3901 ggcggcgagg acgtgcgcat cctctcgttt ccggcacgat attcgctgtc gacggcgagg
     3961 acggcgttct cgaggttctc gagatagcga ttggcctggt aaatgacacc tttcgcgcgc
     4021 tgggcctcga ccttcgtcgt cttcgcccac tcggcggcga agcgaacgac attgtccgcc
     4081 tcgcggcggc cggtggaggc ggcctcggtg atcagaacgg cc
//
LOCUS       DASWNU010000012         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_259756, whole genome shotgun sequence.
ACCESSION   DASWNU010000012 DASWNU010000000
VERSION     DASWNU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259756"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VGB57_00235"
     CDS             <1..817
                     /locus_tag="VGB57_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0129801.1"
                     /translation="NNSSDRTCALAEAAGATVRTERMQGKGNVVRRMFADIEADIYVM
                     ADGDSTYDASVAPELVRRLVEERLDMVVGARKSAVEEAYRRGHRLGNRLFTGILAGLF
                     GRSFTDIFSGYRVFSRRFVKSFPALSRGFETETEISVHALELAMPVAEVVTAYGARPE
                     GSVSKLSTYRDGWRIMRTILHLFRIERPVLFYGGFAAFLAALGLVLAAPLIVTYAKTG
                     FVPRFPTAILVTGMMVVAFLSFGVGLILDTVVRGRREVRRLAYLSFPAVGEGG"
     gene            complement(1262..1777)
                     /locus_tag="VGB57_00240"
     CDS             complement(1262..1777)
                     /locus_tag="VGB57_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0129802.1"
                     /translation="MRTLLLGPFLLLCGCGAGDEGGGKTSPAVAEPAAAPLSTARQPP
                     DAARDEAELIRLEREYARALVERDREFLMRFYASDWRGGNWMGFWSKSTMLKSVLNAR
                     YVIKSMAVRDLKVRLMGDVAVVQGVDEEVTSVDGRDTSGKWAFTDIFARRDGIWVAVA
                     SHTSEIKPTGE"
     gene            2042..2233
                     /locus_tag="VGB57_00245"
     CDS             2042..2233
                     /locus_tag="VGB57_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129803.1"
                     /translation="MEIRKVQAEAMRQRAQSLREAACGFSSGVSRTLRELADAIDEEA
                     ALLEARAGSGGEGEADPSA"
     gene            complement(2415..>3075)
                     /locus_tag="VGB57_00250"
     CDS             complement(2415..>3075)
                     /locus_tag="VGB57_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129804.1"
                     /translation="LMAAAAAALLAAPAASMAAPLVVRSAGPSAKSYPPGRAVGENTP
                     VVLKAGDVVTVLAPSGARTLRGPGTFKVAGGGAAPAFNARSRFSAMRGPEVPPSPGLW
                     DVDVSKSGNFCLADPAQVILWRPAAAAEATLQVIPATGAGQPVAWPAGRLVQRWPQQL
                     PIADDAEYSLKWADAGEPTRLRFKTLAAVGDDPTILAKALIDNGCQAQLDLLLKAAPQ
                     E"
BASE COUNT          462 a         1036 c         1060 g          517 t
ORIGIN      
        1 caataacagc tcggaccgca cctgcgcgct cgccgaggcc gccggagcga ccgtccgcac
       61 cgagaggatg cagggcaagg gcaatgtcgt gcgccgcatg ttcgccgaca tcgaggccga
      121 catctacgtc atggccgacg gcgattcgac ctacgacgcc tcagtcgctc cggagctcgt
      181 ccggcgtctc gtcgaggagc ggctcgacat ggtcgtcggc gcccgcaagt ccgcggtcga
      241 ggaagcctat cggcgcggcc atcgcctcgg caaccggctg ttcaccggaa tcctcgccgg
      301 cctgttcggc cgaagcttca ccgacatctt ctccggctac cgggtcttct cgcgccgctt
      361 cgtcaagagt ttcccggcgc tgtcgcgagg cttcgagacc gagaccgaga tcagcgtcca
      421 cgcgctggag ctcgcaatgc cggtcgccga agtcgttacc gcctacggcg cccggcccga
      481 gggctcggtg tcgaagcttt ccacctatcg cgacggctgg aggatcatgc gcacgatcct
      541 tcatctcttc cggatcgagc gcccggtcct gttctacggc ggcttcgcgg ccttcctggc
      601 ggcgcttggc ctggtcctcg ccgcgccgtt gattgtcacc tacgccaaaa ccggctttgt
      661 gccgcgcttc cccacggcca ttctggtcac cggaatgatg gtcgtcgcct tcttgagctt
      721 cggggtgggg ctgatcctcg acaccgtggt gcgggggcgg cgggaggtcc ggcggctggc
      781 ctatctgagc ttcccggcgg tcggggaggg cggttgaccc tggagcgatt cacgggtggc
      841 tgcctgtgcg gcaatgtccg gatcgcggcg tcggggcgcc cgtaccgggt cggcctttgc
      901 cactgcctcg actgccgcaa gcatcacggg gccctgttcc acgcctccgc catctttccc
      961 cgggaggcgg tgagcatcga gggcgaaacg cgcgattatg gcgggcgctc tttctgtccg
     1021 cgctgcggct catcggtttt cgggcgaacc ggagacgaga tcgaagtgaa tctgggatcg
     1081 ctggataccc cggaccagct cattccgact tacgaaagct gggttgtccg ccgcgaatcc
     1141 tggttgcctc gatttccgct cgcaaggcgc tacgagcgcg atcgcgtccc gggggggcgc
     1201 ttcgaggatt aggcccggcc cgacgcgact gcgccgcaac ccactatgcg gggcgcgccg
     1261 cttactcccc ggtcggcttg atttcgctgg tgtggctcgc gacggccacc cagatgccgt
     1321 cgcggcgggc gaagatgtcg gtgaaggccc atttgccgct ggtgtcccgg ccgtcgacgg
     1381 aggtgacttc ctcgtcaacg ccctggacga ccgcgacgtc gcccatgagc cggaccttga
     1441 ggtcgcgcac cgccatcgat ttgatcacgt agcgcgcgtt gaggacggat ttcagcatgg
     1501 tcgatttgga ccagaagccc atccagtttc cgccccgcca gtcggatgcg tagaaacgca
     1561 tcaaaaactc tcgatccctc tcgacaaggg cgcgggcata ttcgcgttcg aggcggatca
     1621 gctcggcctc gtcgcgagcg gcgtcgggag gctgcctcgc cgtcgaaagg ggcgccgcgg
     1681 ccggttcggc gaccgccggg ctggtcttcc cgccgccctc gtcgccggcc ccgcacccgc
     1741 aaaggagcag gaaagggccg aggagaagcg tccgcatggc gggcgctatg cgtcacgccg
     1801 cggcgcagcc gcaagagcgg ccgccgattt tcctcgtcgc tggacgatcg cgcccttcac
     1861 ccgacggggg agccgcggca gcgaaatgga agcaccgccg gccaactcga tcctttgcaa
     1921 tcagtgcagc cccggaactc gtcccaaatg gcggagcggg gatatagaaa tccgaaatac
     1981 acttgcggct attcgattat cgtatcacca gacggtcatt gcccggacga ggtgcgcgtc
     2041 tatggaaatc cggaaggtcc aggccgaagc gatgcgccag cgcgcccaga gccttcgcga
     2101 ggccgcctgc ggcttttctt caggcgtttc ccgaacgttg cgggagcttg ccgacgcgat
     2161 cgacgaggaa gcggccctgc tcgaggctag ggccgggagc ggcggagaag gggaggcgga
     2221 cccttccgct tagccttcga tccaatcatg gcagcaattt caagggttta agttcgccgc
     2281 gggatatgct agggcgacct caccttgagt tgaatggctt cctctcacgg cgaggccggg
     2341 cacgacgtga cgacggagtg cccggcctct tttttttgcc ggcctgcgat tcgcgccggc
     2401 cgacgctgcc gtccttactc ctgcggcgcg gccttgagca gcaggtcgag ctgggcttgg
     2461 cagccattgt cgatcagcgc tttggccagg atcgtgggat cgtcgccgac ggcggcgagc
     2521 gtcttgaacc ggagccgggt cggctcgccg gcatcggccc atttcagcga atattcggcg
     2581 tcgtcggcga tcggcagctg ctgcggccag cgctgcacca gccgcccggc aggccaggcg
     2641 acgggctggc cggccccggt cgccggaatg acctggaggg tcgcctcggc ggcggcggcg
     2701 ggccgccaca ggatgacctg ggcggggtcg gcgaggcaga aattaccgct cttcgagacg
     2761 tcgacgtccc acaggcccgg cgacggcggc acttccgggc cgcgcatggc gctgaaccgc
     2821 gagcgcgcgt tgaaggccgg ggcggcgcct ccgccggcca ccttgaacgt gccgggcccg
     2881 cgcagggtgc gggcgccgga cggggcgagc accgtcacca cgtcgcccgc cttgaggacc
     2941 accggcgtgt tctcgcccac ggcgcgaccg ggcggatagg attttgccga aggcccggcc
     3001 gagcgaacga cgagcggggc cgccatcgaa gcggcggggg ccgccagcag ggcggccgcc
     3061 gcggcggcca tcaac
//
LOCUS       DASWNU010000013         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_311628, whole genome shotgun sequence.
ACCESSION   DASWNU010000013 DASWNU010000000
VERSION     DASWNU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311628"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VGB57_00255"
     CDS             complement(<1..456)
                     /locus_tag="VGB57_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEY0129805.1"
                     /translation="MRREWGTALRALRRLLANGDDTVQVFRIMRALNGDTSHRNYRRL
                     IASAEGGRLAYERVELASRFCDRAWIESFAEGSVGAAYRAFLDRTGYSADGLAEISVA
                     ETEIPANVEHPYAWFGRRERDVHDIWHVLTGYEADEPLGEACLVAFSYAQ"
     gene            complement(584..1252)
                     /locus_tag="VGB57_00260"
     CDS             complement(584..1252)
                     /locus_tag="VGB57_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129806.1"
                     /translation="MHPRPSSLRRTSRRGAALAALLVAQPAAADDPRKVAVGPDSPKG
                     ALLFKVPPAPVNHVLLFTRIGASPTNANWVLIKGRPVAEGDRFIVTALPPGRYRLDSV
                     HLQRWWLGCLHAAVPTFTIEPGKIAYLGSLDTRLTLASISRSAQAHKNLTASLGQWYR
                     YRIEAGIPRLADRDPEALARAESFARREMPRSSGPVALAELGPGQDSGSTQSQLAARC
                     NYAG"
     gene            complement(1266..1697)
                     /locus_tag="VGB57_00265"
     CDS             complement(1266..1697)
                     /locus_tag="VGB57_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129807.1"
                     /translation="MIEPEAPRTWAYAAALPVTIVCLALANFVIAYLGGWANIEAAKP
                     GAVAIGFVQGLAGALIAGVASSQLFRSARPADFWLKWIVIVAAALFAVVIVWAIVIGK
                     SDRALTWMTFAGVASLIGTEMGRRMAKALARRSESVGSLPG"
     gene            complement(1759..2388)
                     /locus_tag="VGB57_00270"
     CDS             complement(1759..2388)
                     /locus_tag="VGB57_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129808.1"
                     /translation="MDVEIEPPHVHHHGTGHAWLDKVLPVSALFVSFISIMVAWHHGE
                     VMQELVHQNERLVQANSLPHLEIAGSNTNDAGEARVALHVTNAGVGPAEIRSVQVLVD
                     GRPVARLPELLRACCGGEAKDYRGIVSSTLAGSMIRPGETLSYIRFAATPANAEAWTA
                     LDKARTTGRIETRICYCSVFDECWQRSSGGGNRPIPIRQCPVPQPQYTE"
     gene            complement(2408..3520)
                     /gene="dnaN"
                     /locus_tag="VGB57_00275"
     CDS             complement(2408..3520)
                     /gene="dnaN"
                     /locus_tag="VGB57_00275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0129809.1"
                     /translation="MKATIERATLLKSLGHVQSVVERRNTIPILSNVLIEATEGGGLR
                     LMATDLDLQIVETVEANVTQPGATTVSAHTLFDIARKLPEGSQVELNAAEGKMQVNAG
                     RARFNLSTLPRDDFPVIAEGDLPTRFELPAATLRQIIDKTRFAISTEETRYYLNGIFL
                     HVSDEAQPVLKAAATDGHRLARVTVPRPAGAEGMPDVIIPRKCVAELRKLLDELEGTV
                     QISLSETKVRFGLGSAILTSKLIDGTFPDYSRVIPTANDKLLRIDPRSFEEGVDRVAT
                     IASEKTRAVKLALDRDRVTLSVTSPENGTAAEEVPGDYNADGLEIGFNARYLLDILGQ
                     IEGDVVEVHLADAAAPTLLRENDKAAALYVLMPMRV"
     gene            3693..3920
                     /locus_tag="VGB57_00280"
     CDS             3693..3920
                     /locus_tag="VGB57_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0129810.1"
                     /translation="MTVEEIFASALGVDRARVTDDLTYGSIKEWDSVAHMALISALEQ
                     EYDVMIETDDVIDMSSVAVAKTILHKYGVPA"
     gene            3941..>5337
                     /locus_tag="VGB57_00285"
     CDS             3941..>5337
                     /locus_tag="VGB57_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0129811.1"
                     /translation="MAFWDLGAPAGDIALIGPGGETRSYGALAAAADSLASRLPERAG
                     RSLGFLILQPSFEAVAAYLGCLRSRRHVPLLVQQGVNPRLLARLEEIYRPDWMILPAG
                     GEAQAGYCEAWTGDTDSIWAREAVAEEAPLHADLALLLSTSGSTGSEKLVRLSRGALA
                     SNAASIVDYLEMRSDDRTVTTLPLGYSFGMSMLNSHLEAGASILLTGESLMSRDFWRL
                     AAEQKITSLSGVPSTFEMLRRVGLEKRGLDRLRTLTQAGGAMRPDLIRAFDALARDNG
                     WRLFVMYGQTEAAPRISYVPPERLPDKVGSIGIAIPGGALHVDPATSELIYRGPNVMM
                     GYAERREALARGDECGGTLRTGDLGEMDRDGYFYLKGRLKRFIKISGARINLDSVEAG
                     LAERLGTPIVCTGSDDSLCVWLLDESTAAEDEVLASLRETFDIYPGLAGVSRIATFPL
                     TANGKIDYRSLALRS"
BASE COUNT          862 a         1742 c         1846 g          887 t
ORIGIN      
        1 ctgggcgtaa gagaaagcga ccaggcacgc ctccccgagc ggctcgtcgg cctcgtagcc
       61 ggtcaagacg tgccagatgt cgtggacgtc gcgctcgcgc cggccgaacc aggcataagg
      121 atgctcgaca ttggccggga tctcggtctc ggcgacgctg atctcggcga ggccgtcggc
      181 ggaatagccg gtgcggtcga ggaaggcgcg gtaggcggct ccgacggagc cttcggcaaa
      241 ggactcgatc caggcgcggt cgcagaacct cgaagcgagc tcgacccgct cataggccag
      301 cctcccgcct tcggcgcttg cgatcaacct gcggtagttg cggtgcgagg tgtcgccgtt
      361 gagcgcacgc atgatccgga agacctgcac cgtgtcgtcg ccgttggcga gcagccggcg
      421 cagggccctg agggccgtgc cccattcgcg cctcatcggc tccgcgggat tgaaggactg
      481 ggtagccaca ggcgactccg ctgctgacac ggatgtcagt gtggcatccc ccaccgccga
      541 gatcaatatg cgcgtcggag gcgcgcgggc cgcgccgtcc gcttcatccc gcgtaattgc
      601 acctcgccgc caattgggac tgggtcgagc ccgaatcctg cccgggccca agctcggcca
      661 atgcgaccgg gcccgagctt ctgggcatct cgcggcgagc gaagctttcc gcccgcgcga
      721 gtgcctccgg gtcgcgatcg gccagccggg gtatgccggc ttcgatccgg taacggtacc
      781 actggcccag cgatgcggtg agattcttgt gcgcctgggc gcttcgcgag atgctcgcca
      841 gggtcaggcg cgtatccagg gatccgagat aggcgatctt gcccggctcg atggtaaagg
      901 tcggcacggc cgcgtgcagg cagcccagcc accagcgctg caggtggacc gaatcgagcc
      961 ggtagcggcc gggcggaagc gccgtgacga tgaagcgatc gccctccgcg accggcctgc
     1021 ccttgatcag cacccaattg gcgttggtcg gggaggcccc tatgcgcgtg aacaggagga
     1081 cgtggttgac gggcgccggg gggactttga agagcagtgc ccctttcggg gagtcgggac
     1141 cgacggcgac cttgcgggga tcgtcggctg ccgcgggctg agcgacgaga agcgccgcca
     1201 gcgccgcccc acgtcggctt gtcctccgca gggaagaagg ccggggatgc acgcctgcac
     1261 cccgttcaac cgggaaggga gccgacactc tcggaccggc gagccagtgc tttcgccatc
     1321 cggcgtccca tctcggtgcc gataagcgac gcgacgccgg cgaaggtcat ccaggtcagg
     1381 gccctgtcgg acttgccgat gacgatggcc cagacgatca cgaccgcgaa caaggccgcg
     1441 gcgacgatga ctatccattt gagccagaaa tccgccgggc gggccgacct gaacagctgg
     1501 ctggatgcaa cgcctgcgat caacgccccg gccaggccct ggacgaagcc gatggcgacg
     1561 gcaccgggct tggccgcttc gatgttcgcc cacccgccaa gataggcgat gacgaaattg
     1621 gcgagggcga gacagacgat cgtgacgggc aaggccgccg cataggccca cgtcctgggc
     1681 gcttcgggct cgatcatcgc acccacatcc gcatcagcac gtaaagctcg gccgccatgt
     1741 cattctcccg gatcacggtc attcggtata ttgcggctgc ggaaccgggc attgccggat
     1801 cgggatcggg cggttcccgc cgccggagga gcgttgccag cattcgtcga agaccgagca
     1861 atagcagatc cgggtctcga tccggccggt cgtgcgggcc ttgtcgagag cggtccacgc
     1921 ctccgcattg gccggcgtgg ccgcgaacct gatgtaggag agggtctcgc ccggccggat
     1981 catggagccg gccagagtcg agctgacgat gcctcgataa tccttggcct cgcccccgca
     2041 gcaggcccgc aacaactcgg gcaggcgagc taccggacgg ccgtcgacca gcacctgaac
     2101 ggaacggatt tcggcgggtc cgacaccggc attggtgacg tgaagagcga ccctcgcctc
     2161 ccccgcgtcg ttcgtattgc tgcccgcgat ctcgagatgc ggaagcgaat tggcctgcac
     2221 cagccgctcg ttctgatgga ccagctcctg catcacttcg ccatgatgcc aggcgaccat
     2281 gatcgagatg aaggacacga agagcgcgct gaccggaagc accttgtcga gccaggcatg
     2341 gccggtgccg tgatgatgca cgtggggagg ttcgatttcg acgtccaccg cctcttcgtc
     2401 cttccgctca cacccgcatc ggcatcagca cgtagagcgc cgccgccttg tcgttctcgc
     2461 gcagcagggt cggggcggcg gcgtcggcga ggtggacctc gacgacatcg ccttcgatct
     2521 ggcccaatat gtcgagcaga tagcgggcgt tgaagccgat ctcgaggccg tcggcattat
     2581 agtcgcccgg cacctcctcg gcggcggtgc cgttctccgg gctggtgacc gagagcgtga
     2641 cccggtcgcg gtcgagcgcc agcttgacgg cgcgggtctt ctcgctggcg atggtggcga
     2701 cgcggtcgac gccctcctcg aagctgcgcg gatcgatgcg caggagcttg tcgttggcgg
     2761 tcgggatgac gcggctgtaa tcggggaagg tgccgtcgat cagcttggag gtgaggatgg
     2821 cgctgcccag gccgaagcgg accttggtct cggagagcga gatctggacg gtgccctcga
     2881 gctcgtcgag cagcttgcgc agctcggcga cgcatttcct cgggatgatg acgtcgggca
     2941 tgccctcggc gcccgccggc ctgggcacgg tgacccgggc gaggcggtgg ccgtccgtcg
     3001 ccgcggcctt gagcaccggc tgcgcctcgt cggagacgtg gaggaagatg ccgttcagat
     3061 aatatcgcgt ctcttcggtc gagatggcga agcgggtctt gtctatgatc tggcgcaggg
     3121 tcgccgccgg cagctcgaag cgggtcggaa ggtcgccctc ggcgatgacc gggaagtcgt
     3181 cgcgcgggag agtggagagg ttgaagcggg cgcggccggc attgacctgc atcttgcctt
     3241 cggccgcgtt cagctcgacc tggcttcctt cgggaagctt gcgggcgatg tcgaacaagg
     3301 tgtgggcgga gacggtggtg gctccgggct gggtgacgtt ggcctcgacc gtctccacga
     3361 tctggaggtc gagatcggtc gccatcagcc tcaggccgcc gccctcggtc gcctcgatga
     3421 ggacgttgga gaggatcggg atcgtgttcc tccgctccac cacggactgg acgtggccga
     3481 ggctcttgag gagcgtagcc cgttcgatcg tcgccttcat caggtccccc aaattcacgt
     3541 gtgagggagt attagagcgg cgcttcgccc cccgctaggg tttagtgcgc gcggcggcaa
     3601 aaaggtcgtt cgggccaagg gttgagcgcg aggggcttct cctctattcg cggcgccgac
     3661 gaggggcccg gctgggctat ctgaggtaaa aaatgacagt tgaagagatt ttcgccagcg
     3721 ctctgggcgt cgaccgcgcg cgggtgaccg acgatctgac ctacggttcc atcaaggaat
     3781 gggattccgt cgcgcacatg gcgctgatct ccgcgctcga gcaggaatat gacgtgatga
     3841 tcgagacgga cgacgtcatc gacatgagca gcgtcgccgt cgccaagacg atcctgcaca
     3901 aatatggcgt tcccgcctga ggccgaaaga ggcgctcgca atggcattct gggacctcgg
     3961 cgcgccggcc ggggacatcg cgttgatcgg ccccggcggc gagacccgct cctatggggc
     4021 gctggccgcc gccgctgact cgctggcctc caggctgccg gaacgcgccg ggcggtcgct
     4081 cggcttcctc atccttcagc cgagcttcga ggcggtcgcc gcctatctcg gctgcctgcg
     4141 ctcgcgccgg cacgtgccgc tgctcgtcca gcagggcgtc aatccccgat tgctcgcccg
     4201 tctggaggag atctaccggc ccgactggat gatccttccg gcgggcggcg aggcacaggc
     4261 cggatattgt gaagcctgga cgggggacac ggactcgatc tgggcccggg aagcggtggc
     4321 cgaggaggcg ccgctgcatg ccgatctggc gctgctcctg tccacctccg gctcgaccgg
     4381 ctccgagaag ctggtccgcc tctcccgcgg cgcgctcgcc tccaacgccg cttcgatcgt
     4441 cgactatctg gagatgcggt ccgacgaccg cacggtcacg accctgccgc tcggctattc
     4501 tttcggaatg tcgatgctca attcccatct cgaagcgggg gcgtcgatcc tgctcaccgg
     4561 cgaaagcctg atgagccggg acttctggcg gctggcggcc gaacagaaga tcacctcgct
     4621 gtccggagtc cccagcacct ttgagatgct tcgccgcgtc ggcctcgaga aacgcgggct
     4681 cgaccgtctg aggaccctga cccaggcggg cggggcgatg cgtccggacc tcatccgggc
     4741 gttcgacgcg ctggcacggg acaatggctg gcggcttttc gtcatgtacg gccagaccga
     4801 ggccgcgccg cggatcagct acgtgccgcc cgagcgcctc cccgacaagg tcggaagtat
     4861 cggtatcgcc attccgggcg gagcgctcca cgtcgatccc gccacctccg agctgatcta
     4921 tcgcggtccc aacgtgatga tgggctatgc cgagcggcgc gaggccctgg cccgaggcga
     4981 cgaatgcggc ggaaccctgc gcaccggcga tctcggcgag atggaccgcg acggctactt
     5041 ctacctgaag gggcggctca agcgcttcat caagatctcc ggcgcgcgca tcaacctcga
     5101 ctcggtcgag gccggcctgg ccgagcggct cggaaccccg atcgtctgca ccggatcgga
     5161 cgacagcctc tgcgtctggc tgctggacga gagcacagcg gcggaggacg aggtgctcgc
     5221 cagcctgcgc gagaccttcg atatctaccc cggcttggca ggcgtgtcgc ggatcgccac
     5281 tttcccgctg acggcgaacg ggaaaatcga ctatcgctcg ctcgccctcc gctcgaa
//
LOCUS       DASWNU010000014         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_467355, whole genome shotgun sequence.
ACCESSION   DASWNU010000014 DASWNU010000000
VERSION     DASWNU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467355"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1504)
                     /locus_tag="VGB57_00290"
     CDS             complement(<1..>1504)
                     /locus_tag="VGB57_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0129812.1"
                     /translation="AAETALRDGLMRYDRGKGWRDPGLKVDVSGDWRGQLARVPFGVG
                     YDDWRPAVVLSREGQSATIGFADGSTGDLPSYAAAMPKRGVGGAAFNFLRPGMVIAVK
                     REGGVWSLRSIPEIKGGMVVEEVATGRVLAMQGGFDARGDDFNRAVQAQRQPGSTFKP
                     IVYSAALDNGMTPASIIVDGAFCVWQGAGLGNKCFRNFSGGNAGPQTMRWGIEQSRNL
                     MTVRTANQTGMDKVTRLAKIMGVGDYPNYLAIALGAGDTTVLKMTNAFAILANQGRAL
                     KPSLIDYVQDRNGKVIYRADTRPCDGCNARDWDGKPMPRPPLRTKQLLDPLTAYQMVH
                     ILEGVVQRGTAQVLRDLKRPLFGKTGTTSGPTNVWFIGGSADIVAGVYMGFDHPRPMG
                     GYAQGGTLAAPIFKQFAQVAFKDMPVLPFRAPSGIRMVRIDRRSGKKVFGAWPTDDPK
                     AAVIWEAFKPESEPRRSIRRDEMANRPPEKAAARKVRAARTAPVQRDSDFL"
BASE COUNT          215 a          517 c          523 g          249 t
ORIGIN      
        1 caagaagtcg ctatcccgct gcaccggggc cgtccgcgcc gccctgacct tgcgcgccgc
       61 cgctttctcc ggcggccggt tggccatctc gtcgcggcgg atcgagcggc gcggctcgct
      121 ttcgggcttg aaggcctccc agatcaccgc cgccttggga tcgtcggtcg gccaggcgcc
      181 gaacaccttc ttgcccgaac gccggtcgat ccggaccatt cggatcccgg aaggggcccg
      241 gaacggcagc accggcatgt ccttgaacgc gacctgcgcg aactgcttga agatcggcgc
      301 cgcgagcgtg ccaccctggg cgtagccgcc catcggccgg gggtggtcga agcccatata
      361 gaccccggcg acgatgtcgg cgcttccgcc gatgaaccag acgttggtcg ggcccgaagt
      421 ggtcccggtc ttgccgaaca gcggccgctt caggtcgcgg agcacctgag cggtgccgcg
      481 ctggacgacg ccttcgagga tgtggaccat ctggtaggcg gtgagcgggt cgagcagctg
      541 cttggttcga agcggaggcc ggggcatcgg cttgccgtcc cagtcgcgcg cgttgcagcc
      601 gtcgcagggc ctcgtgtcgg cccggtagat gaccttgccg ttgcggtcct ggacatagtc
      661 gatcaggctc ggcttcagcg cccggccctg gttggcgagg atggcgaagg cgttggtcat
      721 cttgagcacg gtggtgtcgc cggcgcccag cgcgatggcg agataattgg gatagtcgcc
      781 gacgcccatg attttggcga gccgggtgac cttgtccatt ccggtctggt tggctgtgcg
      841 gaccgtcatc aggttgcgcg actgctcgat cccccagcgc atggtctggg gcccggcatt
      901 gccgcccgag aagttgcgga agcatttgtt gccgagcccc gcgccctgcc agacgcagaa
      961 ggcgccgtcg acgatgatcg aggcgggggt cattccgttg tcgagcgcgg ccgaatagac
     1021 gatcggcttg aaggtcgatc cgggctggcg ctgggcctgg acggcccggt tgaaatcgtc
     1081 gccgcgggcg tcgaagccgc cctgcattgc gagcacccgg ccggtcgcca cttcctcgac
     1141 gaccatcccg cccttgatct cggggatcga gcgaagcgac cagacgccgc cctcccgctt
     1201 gaccgcgatc accattcccg gccgaaggaa gttgaaggcg gcgccgccga ccccgcgctt
     1261 gggcatcgct gcggcatagg aaggcaggtc gccggtcgag ccgtcggcga agccgatcgt
     1321 cgcgctctgg ccctcgcgcg acagcaccac cgccggccgc caatcgtcgt agccgactcc
     1381 gaacggaacc cgggccagct gtccgcgcca gtcgccggag acgtcgacct ttagcccggg
     1441 atcccgccag cccttgccgc ggtcgtagcg catcaggccg tcgcgaagcg ccgtttccgc
     1501 ggcc
//
LOCUS       DASWNU010000015         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_104052, whole genome shotgun sequence.
ACCESSION   DASWNU010000015 DASWNU010000000
VERSION     DASWNU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104052"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..560)
                     /locus_tag="VGB57_00295"
     CDS             complement(3..560)
                     /locus_tag="VGB57_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0129813.1"
                     /translation="MGRAVRRVVTGHDAKGRSIVQEDGPPARVATLGGDSGTTFHEVW
                     NTQLTPAPIDRSSGEPAEPGISLLPPAGGTRIRILDIPPDDGTIAALPREAVRALFEA
                     IGAGHALAENPPHPLMHRTETVDYGIVLEGELVLILDESETVVRAGDIVIQRGTSHAW
                     ANRSTSNARIAFILVDGRFEEGLGG"
     gene            649..969
                     /locus_tag="VGB57_00300"
     CDS             649..969
                     /locus_tag="VGB57_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129814.1"
                     /translation="MSATRLLRWLTLFALLVAPLAMIGGSPATAHVAAVLGGHCAETE
                     KPVEAPPSAPVDCMIACAGCLPTQAGTLAVRPRLEPAARPGPIDFRLSGLNPEAATPP
                     PRIS"
     gene            1220..2374
                     /locus_tag="VGB57_00305"
     CDS             1220..2374
                     /locus_tag="VGB57_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129815.1"
                     /translation="MTGALGPYPASREASGTAWQPDSSPHGGLHLRSGDWTLMGHAAL
                     HAVYDRQEGPRGGEKAFVSGMAMGMARRPLGSGTLQLRASFSPDPLMGRRGYPLLLAA
                     GETANGTDPLVDRQHPHDLFMELSASYSLPLSKGASLFLYGGLPGEPAFGPPTFMHRV
                     AAMDSPLAPISHHWLDSTHIVFGVVTAGLIVGDARLEASRFNGREPDQRRFDIETGPL
                     DSTALRLSWNPAPSLALQASWARLIGPEQLEPDENETRWSASALHTRRLGDKGWWSTT
                     FAWGRRTKAHADLDALVLESAVGLGRWTLFGRAERIETDELTRIGGHHGPVYDVGKAS
                     LGAIRDFPLARHVKLGLGGLWAFDFVPRALEPFYGGDPKGGMLFLRLRVD"
     gene            2379..2858
                     /locus_tag="VGB57_00310"
     CDS             2379..2858
                     /locus_tag="VGB57_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0129816.1"
                     /translation="MSRSRRLVRIVFTFLHGLRRALWKVAGPGRPGVHALPLTERGRV
                     VLVLLTYAPGWRLPGGGRGRKEAPEAAVLRELREEIGLVSHGRIERLEDGRPDSVLPG
                     DRSAFFLVRDVVYRPRRSLEIEAVREFDPRRLPDDISPWTAHIVQKWTQGPYPSSRT"
     gene            complement(2983..3621)
                     /locus_tag="VGB57_00315"
     CDS             complement(2983..3621)
                     /locus_tag="VGB57_00315"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0129817.1"
                     /translation="MDALVEVPEPGDDQADEARPQAPIWAEPRHLFFFRLGALLGEGE
                     QRPVEILALAESGAIVRIGESLQEGARLVIELNSHHRFPVRVEWSSDTLAGLEFEDRD
                     LVRETLAARDRAHPYRAPRLELAAEVVLRLGETCVTAQARDVSEGGIKVELAEPGHVG
                     REASVIVPGMPPVAGRILWCRDGRAGISFERPLPFRSLIDCLTRKPVEQPGT"
BASE COUNT          520 a         1364 c         1269 g          600 t
ORIGIN      
        1 cttcagcctc caagcccctc ctcgaacctg ccgtccacca gtatgaaggc gatcctggca
       61 ttgcttgtag agcggttggc ccaggcgtgg ctggtgccgc gctggatgac gatgtcaccg
      121 gcccgaacca cggtctcgct ctcgtcgagg atgaggacga gctcgccttc gaggacgatt
      181 ccataatcca ccgtctcggt ccggtgcatc agcgggtggg gcgggttttc ggccagggca
      241 tggccggcgc cgatcgcctc gaaaagcgcg cgcaccgcct cacggggcag agcggcgatg
      301 gtgccgtcgt cgggcgggat gtcgaggatc cggatccggg tgcccccggc cggcggcaga
      361 agcgagatgc cgggctccgc cggctcgccc gaggatcgat cgatgggggc gggggtcagc
      421 tgcgtgttcc acacttcgtg gaaagtcgta ccgctgtcgc cgccgagggt cgccacccgc
      481 gccggcgggc catcctcctg gacgatcgag cggcccttcg catcgtggcc ggtcacgacc
      541 cggcgaacgg ctcttcccat cgcgttcctc cctcctcctg aaccttcgcg gcctgcggga
      601 aagcgggcgg cgttgcaagc cgccggggag gggtctatct gcgcgggcat gtcggcaacc
      661 cgcctgctca ggtggctcac ccttttcgcg ctgctggtcg ccccgctcgc catgatcggc
      721 ggttcgccgg ccacggccca cgtcgcggcg gtgttgggcg gccactgcgc cgagaccgag
      781 aagcccgtcg aggctccgcc ttcggctccc gtcgactgca tgatcgcctg cgccggttgc
      841 ctgccgaccc aggccgggac gctcgcggtc cggccccggc tcgagcccgc ggcccggccg
      901 gggccgatcg atttccgcct cagcggcctc aaccccgaag ccgcgactcc gccgcctcga
      961 atctcctgac gctgcagcac tcgccgtcat gacggccatg actcccactg agtaagcctc
     1021 agggcttcgg agcattacga tgaatgtgaa acttgccgcc ggggccctcg ccctggcgct
     1081 cgccgcgtcg cctgcgttcg cgcagcacga aggccatgac accccggaga cggtccctcc
     1141 ggcgccggca gaccctgcgc atgagggcca cccaatgcca gggccggccg accctcccgc
     1201 cgcccgggag ggccatgcca tgaccggcgc cctcggcccc tacccagcca gccgggaagc
     1261 ctcgggcacc gcctggcaac cggattcctc gccccacggc ggcttgcatc tcaggtccgg
     1321 cgactggacc ttgatgggcc acgccgccct ccacgccgtc tatgatcggc aggaaggccc
     1381 tcgcggcggc gagaaggcct tcgtctccgg aatggcgatg ggcatggccc ggcggccgct
     1441 cggctccgga acgcttcagc ttcgagccag cttcagcccc gatccgctga tgggccgccg
     1501 cggctatcca ctccttctcg ccgcgggcga gacggcgaac ggtaccgatc ccctggtcga
     1561 ccgccagcat ccgcacgacc tcttcatgga gctgtcggcc agctacagcc tgcccttgtc
     1621 gaagggggcg tcgctgttcc tctacggcgg ccttcccggc gagcccgctt tcggtccgcc
     1681 gaccttcatg catcgggtcg ccgcgatgga ttcgcctctg gccccgatca gccaccattg
     1741 gcttgattcc acccacattg tcttcggggt cgtgaccgcg ggcctcatcg tcggcgacgc
     1801 caggctcgag gcgtcccgct tcaacggccg cgagcccgac cagcggcgct tcgacatcga
     1861 gaccggaccg ctcgactcga ccgcgcttcg gctctcctgg aacccggcgc cgtcgctggc
     1921 gctgcaggcg agttgggcgc ggctgatcgg ccccgagcag ctcgagccgg acgagaacga
     1981 gacccgctgg tcggcgagcg ccctccacac gcgccggctg ggagacaaag gctggtggtc
     2041 gaccactttc gcctgggggc gacggaccaa ggcgcatgcc gatctcgacg ccttggtcct
     2101 ggagagcgcg gtcgggctcg gccggtggac cctgttcggc cgcgccgagc ggatcgagac
     2161 ggacgagctg acccggatcg gcggtcatca cgggccggtc tacgacgtcg gcaaggcttc
     2221 gctgggagcg atccgcgact tcccgctggc ccggcacgtc aagctcggcc tcggcggcct
     2281 ctgggccttc gacttcgtgc cccgcgccct ggagccattt tacggcggcg atccgaaagg
     2341 ggggatgctc ttcctgcgcc tcagggtaga ttgagcggat gagccggtcg cgccgcctgg
     2401 tccgaatcgt cttcaccttc ctgcacggtt tgcgccgcgc gttgtggaag gtggccgggc
     2461 ctggccgccc cggagtccac gccttgccgc tgaccgagcg gggcagggtg gttctggtcc
     2521 tcctcaccta cgcgcccggc tggcgcctgc ccggcggcgg ccgggggcgg aaggaggcgc
     2581 cggaagccgc ggttctgcgc gagctccggg aggagatcgg tctcgtctcg cacgggcgca
     2641 tcgagaggct cgaggacggc cggcccgatt ccgtccttcc cggcgaccgc tccgctttct
     2701 tcctggtccg cgacgtcgtc taccggccgc ggcgaagcct ggagatcgag gcggtgcgcg
     2761 agttcgatcc gcggcgactt ccggacgaca tcagcccgtg gaccgcgcat atcgtccaaa
     2821 agtggaccca gggtccgtac ccgtcatccc ggacttgatc cgggatccat gaacaccgcg
     2881 ggtcgggttc gagttcggcg gtgttcaggg attccggcct tcgccggaat gacgggaggg
     2941 agagcccgct tcgccgcccg gtggaagcgt gtgaaagaac ccctacgtcc ccggctgctc
     3001 cacaggcttc cgggtgaggc agtcgatcag gctccggaag ggcagggggc gttcgaacga
     3061 gattccggcc cggccgtcgc ggcaccacag gatccggccg gccaccggcg gcatcccggg
     3121 aacgatcacg ctcgcctcgc gcccgacatg cccgggctcg gcgagctcta ccttgatacc
     3181 gccttccgaa acgtccctgg cctgagcggt gacgcaggtt tcgcccagtc gcaggacgac
     3241 ttccgccgcg agctccagcc gcggcgcccg ataagggtgg gcgcggtcgc gggcggcgag
     3301 ggtttcacgc accaggtcgc gatcctcgaa ctcgaggccg gccagggtat cggacgacca
     3361 ttccacccgt acggggaaac ggtggtggga gttgagctcg atgaccagcc gggccccttc
     3421 ctgaaggctc tcgccgatcc ggacgatggc tccgctttcg gcgagggcca gtatctcgac
     3481 cggtctctgc tccccctcgc cgagcagggc gccgagccgg aagaagaaca aatgccgcgg
     3541 ctcggcccag atcggagcct gcggacgcgc ttcgtccgct tgatcgtcgc ccggctccgg
     3601 cacctccacc aacgcgtcca tcctcgcctc cgctgctagg cccgcctgat gcggccccgg
     3661 ctgctgccgg acgatgaaca ggtgaggcta aggccgtggt aactatccca ggtaacgcgg
     3721 aacttcagtc gcttacgcgc tccggcttgc cct
//
LOCUS       DASWNU010000016         7713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_208134, whole genome shotgun sequence.
ACCESSION   DASWNU010000016 DASWNU010000000
VERSION     DASWNU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208134"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..793)
                     /locus_tag="VGB57_00320"
     CDS             complement(152..793)
                     /locus_tag="VGB57_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129818.1"
                     /translation="MADPDSRATDPGFKLWQIADIRWVHCPRCDGPARLAGGRLTCTR
                     CAYAEGGPKKGRRRLKAVMTAKNNPKCENRSCGAPVPNHGPPVRGGGSDELNVRIVCP
                     TCGHVGDYPAVPIEPLDVRKAHAALRLFHSWPSAAPYLRRRIGAHTLVVYNQQHLDAL
                     ETWLGAEHRQRGPVAGLTMMARLPRWMKAARARDDVLKALGELRKQAESEALW"
     gene            924..2324
                     /gene="fumC"
                     /locus_tag="VGB57_00325"
     CDS             924..2324
                     /gene="fumC"
                     /locus_tag="VGB57_00325"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0129819.1"
                     /translation="MISTATARTETDSFGPIDVPGDAYWGAQTQRSIQNFPFGPRETM
                     PVEIVHALGFVKQAAARVNARIGGLNAELAEAVQQAAGEVASGMHDDQFPLVIWQTGS
                     GTQSNMNANEVIAGRANEILTGNRGGKSPVHPNDHVNKGQSSNDSFPTAMHIAAARAV
                     EGRLIPALQAMHRRLAAQASAWDSIVKIGRTHLQDATPLTLGQEFSGYAAQVEAGIER
                     LRSVQPRVLQLAQGGTAVGTGLNAPEGFAEAFADQVATLTKLRFTSAPNKFEALAAHD
                     TLVELSGVLNVIAVSLTKIANDIRLMGSGPRCGLGELKLPENEPGSSIMPGKVNPTQA
                     EMLTMVAAQVMGNHVAVTVGGLQGHLELNVFKPVIAANLLRSIDLLATGMESFTERTL
                     DGLEPDESRIAELMNRSLMLVTALAPEIGYDNAANIAKNAHEKGLTLKESGIELGLVD
                     EATFDRVVKPEAMLGR"
     gene            complement(2357..2923)
                     /locus_tag="VGB57_00330"
     CDS             complement(2357..2923)
                     /locus_tag="VGB57_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129820.1"
                     /translation="MPSLRSIALAALTALLMTPPATSAGPQPFADGTRGDGARDFDFE
                     IGRWKTELRRLKRPLTGSSEWVSYSGTTTVTRVWGGKANLVELEVEGPAGRLQALSLR
                     LYNPDTREWTLNFANSTGGTLGVPSVGAFRDGRGEFYNQEAFAGRTVLVRFVISDIKA
                     NSVRFEQSFSADGGRSWEPNWIAVDTRL"
     gene            2998..3624
                     /locus_tag="VGB57_00335"
     CDS             2998..3624
                     /locus_tag="VGB57_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0129821.1"
                     /translation="MEQKRPRRPGRPPAYDRDDVVEKAQRLFWKDGAAGVSLDALSEA
                     TGLHKPSLYAAFGGKAGLYVAALDAYIGRGAPDVQGAFARAPLRAALEAFYEADLDVF
                     CGEEGKSGCFLVGTAIQAAAELPEVRSRVEAVFVGLRRLLRVRLERAADEGDLPTGAD
                     MDGLTEILFGTHIALSVEARAGAPRAELRQHYRRVLDFLASLAPSRAA"
     gene            complement(3611..4138)
                     /locus_tag="VGB57_00340"
     CDS             complement(3611..4138)
                     /locus_tag="VGB57_00340"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY0129822.1"
                     /translation="MKPIVTGAALALAAAAAPAHGQELFGGVYVHDVDTGLTKSGIEE
                     GVDLELGWRGDRIGALRAIGGPRPQAFVSVNSAGNTHFAAAGLSWKIGGRAYLRPGVG
                     LAVHTGPGRVVPGDDRIDFGSRILFAPELGLGARISDRLSIEASWVHLSHAQLFGPQN
                     PGLDTIGVRLNFKLP"
     gene            4210..4374
                     /locus_tag="VGB57_00345"
     CDS             4210..4374
                     /locus_tag="VGB57_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129823.1"
                     /translation="MSNDDTAPGDEAAPGTPGTGENICRHCNGTGEHDGIPCPVCDGT
                     GKVTEGLAGG"
     gene            4489..5013
                     /locus_tag="VGB57_00350"
     CDS             4489..5013
                     /locus_tag="VGB57_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0129824.1"
                     /translation="MIRSLLIASAASLALAACATEGDMGAGVAAAGDMTPEQRDAYVQ
                     MAAASDLFEIQSSQLAVSRAQSPDVRAFAQMLIEHHTMTTQQLTAAATAAGTPPSPAL
                     MPMQAEMIAQLQGANGADFDRMYMRQQVPAHQMALALHQNYASNGDTASLRTVAAAAV
                     PIVRQHLDRARALR"
     gene            complement(5115..5684)
                     /locus_tag="VGB57_00355"
     CDS             complement(5115..5684)
                     /locus_tag="VGB57_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006960264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HEY0129825.1"
                     /translation="MSVPHVPRGYWFAALGLVAVMALILLWMGRPPICTCGEVKLWVG
                     TVNGPDNSQHLADWYTPSHIIHGFLFYGLGWLFLRRNPPGDRLIGAVLIEAGWELLEN
                     SRWIIERYREATMALGYNGDAVINSAADVGWMVAGFALARRLPVWATLVLAVAFELLA
                     LAIIRDNLTLNVIMLVAPSDAIRAWQAGA"
     gene            complement(5773..6306)
                     /locus_tag="VGB57_00360"
     CDS             complement(5773..6306)
                     /locus_tag="VGB57_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY0129826.1"
                     /translation="MARLIALLRAVNVGGRKLPMAQLRALCGELGWRDVETYIQSGNL
                     VFAAPGRAEAIEAQLEKAIKDRFGLDVPVMVRTAAQWSDYAAANPFPEVARDEPNRLQ
                     LLVSKRPPDPAAAEKLMERALAGEAVKAAGGALWFHFPAGVGTSKLVPALIDKAAGSP
                     STSRNWRTVLKLQEMAK"
     gene            6354..6986
                     /gene="gmk"
                     /locus_tag="VGB57_00365"
     CDS             6354..6986
                     /gene="gmk"
                     /locus_tag="VGB57_00365"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY0129827.1"
                     /translation="MEIASAFQRRGLLFVLSSPSGAGKSTIARMLIEADDGVAMSVSA
                     TTRPIRPGEVDGRDYHFVDDKGFDSLVAAGHFLEWAHVFGHRYGTLKSEVLKTIEGGR
                     DVLLDIDWQGTQQLKQVDPDIVRVFILPPSMKELERRLRTRGTDSEDVIRRRMERAAA
                     EISHWAEYDYVLINNDAEKCRELVHNILKAERLKATRRVFLHDFVRQLIG"
     gene            7042..7656
                     /locus_tag="VGB57_00370"
     CDS             7042..7656
                     /locus_tag="VGB57_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129828.1"
                     /translation="MIRTVIGGLIAGVIIFVVGFIFWATPLGELAYSKADEAQNAAVQ
                     ASLAQNLVKKDPQTGEMSGTGTYIIPAHNSAAGAVLYARGPIATVHFNTRGYSPDDMS
                     AILPGFIVAVVAGLLIAFGLAAVGGRSFADLARLVVCFSLGFTVWEYLGSPIFNHFGW
                     GYWIYTFIAESVALILAGLVVACWFMPHHERGEAHSTPHPEQSE"
BASE COUNT         1297 a         2660 c         2561 g         1195 t
ORIGIN      
        1 cccctgggcg tgccgcaccc gagcctgcgc atccatccag cttccctcca tcgcgaaaac
       61 tcccggccgg ggccaaggcg cgaagctcct agcatagctc catcgcttcg aaggcccagc
      121 gccggcaacc tcgcactcca aagcgttgat ctcaccataa tgcctcggac tccgcctgct
      181 ttctcagctc ccccaacgcc ttcaaaacgt cgtctcgagc tcgcgcggcc ttcatccagc
      241 ggggaaggcg cgccatcatc gtcaggccag cgaccgggcc tcgctggcgg tgctctgctc
      301 ccagccaggt ctcgagcgca tccagatgct gctggttgta gacgacgagc gtgtgcgcgc
      361 cgatgcggcg gcgcaggtaa ggcgccgcgc tcggccatga gtgaaagagg cgcagggcgg
      421 catgcgcttt tcgcacatcg agaggctcga tgggaaccgc gggataatcg ccaacgtgtc
      481 cgcaggtcgg gcagacgatc cgaacgttca gttcgtccga gccgccgccc cggacgggcg
      541 gcccgtgatt ggggacaggc gcaccgcagg acctgttctc gcatttcggg ttgtttttcg
      601 cggtcatcac cgccttgagg cggcgccggc cctttttcgg gccgccctcg gcgtaagcgc
      661 agcgagtgca ggtcagccgg ccgcccgcca gccgcgccgg gccgtcgcaa cgcgggcaat
      721 gcacccaccg gatgtcggcg atctgccaca gcttgaagcc cgggtcggtt gcgcgggagt
      781 cgggatctgc catcacttac ccccaggcat cgaccgtgca ggcaaccttg tgacagcgac
      841 ggcgaatgtt cgcaatcggg tcgccaggcc catggatctt tccccgcgtg acgggcgcat
      901 ttcccgccgc taaggggctc cgcatgatct ccaccgccac cgcccgcacc gagaccgaca
      961 gcttcggccc catagacgtt cccggcgacg cttattgggg cgcccagacc cagcgttcga
     1021 tccagaattt cccgttcggg ccgcgcgaga cgatgccggt cgagatcgtc cacgctttgg
     1081 gcttcgtgaa gcaggcggcg gcccgggtga acgcccgcat cggcgggctc aacgccgagc
     1141 tcgccgaggc ggtccagcag gcggcgggcg aggtggcgag cgggatgcac gacgaccagt
     1201 tcccgctggt catctggcag acgggctccg gcacccaatc caacatgaac gccaacgagg
     1261 tgatcgccgg ccgagccaac gagatcctca cgggcaatcg cggcggcaag agcccggtcc
     1321 atcccaacga ccatgtcaac aagggccagt cgtccaacga cagcttcccg accgcgatgc
     1381 acatcgccgc ggcccgggcg gtggaggggc ggctgatccc cgccctccag gccatgcacc
     1441 gccggctggc cgctcaggcg agcgcctggg actctatcgt caagatcggc cggacccacc
     1501 tccaggacgc gaccccgctc accctcggcc aggaattttc gggctatgcc gcccaggtcg
     1561 aggccggcat cgagcggctc cgctcggtcc agccgcgggt gctccagctc gcccagggcg
     1621 gaaccgccgt cggaaccggc ctcaacgcgc ccgaaggctt tgccgaggcc ttcgccgacc
     1681 aggtggcgac gctcaccaag cttcgcttca ccagcgcgcc caacaagttc gaggcgctgg
     1741 ccgcgcacga tactctggtc gagctgtccg gcgtgctcaa cgtcatcgcc gtctcgctga
     1801 ccaagatcgc caacgacatc cgcctgatgg gctcggggcc gcgctgcggg ctcggcgagc
     1861 tgaagctgcc ggagaacgag ccgggcagct cgatcatgcc gggcaaggtc aacccgacgc
     1921 aggcggagat gctgaccatg gtcgccgccc aggtgatggg caaccatgtc gcggtcaccg
     1981 tcggcggcct ccaggggcat ctcgagctca acgtgttcaa gccggtcatc gccgccaatt
     2041 tgcttcgctc gatcgacctg ctcgccaccg gcatggaaag cttcaccgag cggacgctcg
     2101 acgggctgga gcccgacgag agccggatcg cggagctgat gaaccgctcg ctgatgctgg
     2161 tgacggcgct ggcgccggaa atcgggtacg acaacgccgc gaacattgca aagaatgccc
     2221 acgagaaggg gctgacgctc aaggagtcgg gaatcgagct cgggcttgtc gacgaggcga
     2281 cgttcgaccg ggtggtgaag cccgaggcga tgctggggcg ctaagaattc ctcccccggc
     2341 aaaagccggg gaggaatcat agccgcgtgt cgacggcgat ccagttcggc tcccagctgc
     2401 ggccgccatc ggcggagaag gactgctcga agcggacgga attggccttg atgtccgaga
     2461 tcacgaacct caccagcacg gtccgcccgg cgaaagcctc ctgattgtag aattcaccgc
     2521 gaccgtcgcg gaaggcgccg acggagggga cgcccagcgt cccgccggtg ctgttggcga
     2581 agttcaaggt ccattcgcgc gtgtccgggt tgtagagccg gagcgacagg gcctgcagcc
     2641 gcccggccgg gccctccacc tcgagctcca ccagattggc cttgccgccc cagaccctgg
     2701 tgacggtcgt ggttcccgaa taagaaaccc actccgacga gccggtcagc ggacgcttga
     2761 gccgccgaag ctccgttttc cagcggccga tctcgaagtc gaagtcacga gcgccgtcgc
     2821 ctctcgtccc gtctgcgaac ggctggggcc cggccgaggt cgcgggaggc gtcatgagca
     2881 gggcagtcag ggcagcgagc gcgatcgagc ggagcgaagg catggaaggt cgccttgtcg
     2941 tgggttggtt ttgtactatt tagaatataa gtgggccgtc aagagatttt gtaccgaatg
     3001 gaacagaaac gcccgcgccg gcccggcagg ccgccggctt atgaccgcga cgacgtcgtc
     3061 gagaaggcgc agcggctctt ctggaaggac ggggcggccg gagtctcgct cgacgcgctg
     3121 agcgaagcga ccggccttca caagcccagc ctctatgcgg ctttcggggg caaggcgggc
     3181 ctctacgtag cggcgctcga cgcctatatc ggacggggcg cgcccgacgt ccagggcgcc
     3241 ttcgccaggg cgccgctgcg ggcggcgctc gaagcctttt acgaagccga tctcgacgtc
     3301 ttttgcggcg aggaagggaa aagcggctgc ttcctcgtcg gaaccgccat ccaggcggcg
     3361 gcggaacttc ccgaggtgcg ctcgcgagtc gaggcggtct tcgtgggcct tcgccgcctc
     3421 ctgcgcgtcc ggctcgagcg ggcggccgat gaaggcgatc tccccaccgg cgcggacatg
     3481 gacgggctga ccgagatact cttcggcacc catatcgcgc tgtcggtcga agcccgcgcg
     3541 ggagcgccga gggcggagct tcggcagcat taccgccggg tgctggattt cctcgcttcc
     3601 ctcgccccgt ctagggcagc ttgaaattca gccggacccc gatcgtgtcg aggcccggat
     3661 tttgcgggcc gaacagctgg gcgtggctga gatggaccca gctcgcttcg atcgagagac
     3721 ggtccgaaat ccgtgcgccg agcccgagct ccggggcgaa caggattcgc gagccgaaat
     3781 cgatgcggtc gtcgcccgga accacccggc ccggtcccgt gtggacggcg aggccgacgc
     3841 cggggcgcag ataggcccgg ccgccgatct tccagctcag cccggccgcg gcgaagtggg
     3901 tgttgccggc gctgttgacc gagacgaagg cctgcggccg cggaccgccg atcgctcgaa
     3961 gcgcgccgat ccggtcgccg cgccagccga gctccagatc caccccttcc tcgatcccgc
     4021 tcttggtcag gccggtgtcg acgtcgtgga cgtagactcc gccgaacagc tcctggccgt
     4081 gggcgggggc cgcggcggcg gctagagcga gggcagcgcc cgtaacgatc ggcttcatgg
     4141 ccgggctaac ccgtcgcgcg cagcgtcggt tccggggaac cgggccccca agcgcgggtt
     4201 gaaaccacca tgagcaacga cgacaccgct cccggcgacg aagccgcccc gggaacgccc
     4261 ggcaccggcg agaatatctg ccgccattgc aacggcaccg gcgagcatga cggcatcccc
     4321 tgcccggtct gcgacggcac cggcaaggtc accgagggac tggccggcgg ctgagcggcc
     4381 gtcgcttcag gaatctccct aacccacatc agagccttgg ttttttcccg gaaccggcgg
     4441 cgcccgcatc cgcttgtcgg ccatcccact gcccgacagg aatcccacat gatccgctcc
     4501 cttctcatcg cttccgccgc ttccctcgcc ctcgccgcct gcgccaccga aggcgacatg
     4561 ggagccggcg tcgccgccgc cggcgacatg acccccgaac agcgcgacgc ctacgtccag
     4621 atggccgccg ccagcgacct tttcgagatc cagtcctccc agctcgccgt ctccagggcc
     4681 cagagccccg acgtccgcgc gttcgcccag atgctgatcg agcaccacac catgaccacg
     4741 cagcagctga ccgccgcggc gacggcggcg ggaaccccgc cgagccccgc cctgatgccg
     4801 atgcaggccg agatgatcgc ccagctgcag ggcgcgaacg gcgccgattt cgaccggatg
     4861 tacatgcgtc agcaggttcc cgcgcaccag atggcgctcg cgctgcacca gaattatgcg
     4921 agcaacggcg atacggcgag cctccgcacc gtcgcggccg ccgcagtgcc gatcgtccgc
     4981 cagcacctcg accgggcgcg ggcgctgcgc tgaggctaag tcatcccggc gcaggctggg
     5041 atcccgggtg gtgaagagcg cagcgctttt cacccgctgg ggccccagcc ttcgccgggg
     5101 cgacggtccg gagatcaggc gccggcctgc caggccctta tggcgtcgct cggcgccacc
     5161 agcatgatca cgttgagcgt gagattatcg cggatgatgg ccagcgccag aagctcgaac
     5221 gcgaccgcca ggaccagcgt cgcccagacc ggcagccgcc tcgccagggc gaagccggcc
     5281 accatccagc cgacgtcggc ggcggagttg atcacggcgt cgccattata gcccaatgcc
     5341 atcgtcgcct cgcggtagcg ctcgatgatc cagcggctgt tctcgagcag ctcccacccc
     5401 gcctcgatca gcaccgctcc gatcagccgg tcgccgggcg ggttgcggcg caggaagagc
     5461 cagccgagcc cgtagaagag gaagccgtgg atgatgtggc tcggcgtata ccagtcggcg
     5521 agatgctggc tgttgtcggg gccgttgacc gtcccgaccc acaatttgac ttctccgcag
     5581 gtgcagatgg gcggccttcc catccagagc aggatgaggg ccatcaccgc gacgaggccg
     5641 agcgcggcga accaatagcc ccgggggaca tgagggacgc tcatcccgtc atcccggcga
     5701 aagccgggac ctcaggacaa gaaggcacga ggggtctccc cgaggccccg gctttcgccg
     5761 gggtgacgga agtcatttcg ccatctcctg aagcttgagg acggttcgcc agttgcggct
     5821 ggtcgacggg gagccggcgg ccttgtcgat caaggcgggt acgagcttcg aggtgccgac
     5881 gcccgccgga aaatggaacc agagcgcgcc gccggctgcc ttgaccgctt ccccggccaa
     5941 ggcccgctcc atcagctttt cggcggcggc aggatcgggc ggccgtttcg agaccagcag
     6001 ctggaggcgg ttcggctcgt cgcgcgcgac ttccgggaag gggttggccg cggcgtagtc
     6061 cgaccattgc gccgcggttc ggaccatgac cggcacgtcg agcccgaacc ggtcctttat
     6121 cgccttttcg agctgcgcct cgatcgcttc ggccctgccg ggggcggcga agaccagatt
     6181 gccgctctgg atgtaggtct cgacgtcccg ccatccgagc tcgccgcaca gggcgcgaag
     6241 ctgagccatc ggcagcttgc ggccgccgac gttgacggct cggagaaggg cgatgagacg
     6301 ggccatgtcc ctacctgctt gacgcatggc ggctcggcaa gccagtagcg cgcatggaaa
     6361 tcgcaagcgc cttccagcgg cggggcctcc tcttcgtcct gtccagcccc tcgggcgccg
     6421 gcaagtccac catcgcccga atgctgatcg aggccgacga cggggtcgcc atgtcggttt
     6481 cggcgaccac ccggccgatc cgcccgggcg aagtcgacgg gcgcgattac cacttcgtcg
     6541 acgacaaggg cttcgattcg ctggtcgccg ccggccattt cctcgaatgg gcgcatgtct
     6601 tcggccaccg ctacggcact ttgaagagcg aggtgctcaa gacgatcgag ggcggccgcg
     6661 acgtactgct cgacatcgac tggcagggca cccagcagct gaagcaggtc gatcccgaca
     6721 tcgtccgggt cttcatcctg ccgccgtcga tgaaggagct ggagcggcgc ctgcgcaccc
     6781 gcggcaccga cagcgaggac gtcatccgac gccggatgga gcgggccgcg gccgagatca
     6841 gccattgggc cgaatatgat tatgtgctga tcaacaacga cgccgagaaa tgccgggagc
     6901 tcgtccacaa catcctcaag gccgagcgat tgaaggcgac tcggcgggtc ttcctccacg
     6961 atttcgtccg gcaattgatc ggctgacttt gccggcgccc cgattgagcg tagtgttcac
     7021 cggcaacaaa aggggatgaa catgatccgg accgtgatcg gcggcctcat cgcgggtgtc
     7081 atcatattcg tcgtgggctt catcttctgg gcgaccccgc tcggagagtt ggcctattcg
     7141 aaagcggatg aagcgcagaa cgccgcggtc caggcctcgc tggcccagaa tctggtcaag
     7201 aaggaccccc agaccgggga gatgagcggc accggcacct acatcatccc cgctcacaac
     7261 agcgccgccg gagcggtgct ctacgcgcgc ggcccgatcg ccacggtgca tttcaacacg
     7321 cgcggctatt cgcccgacga catgtcggcg atcctgccgg gcttcatcgt ggcggtggtg
     7381 gcggggcttc tgatcgcctt tggcctcgcg gcggtgggcg gacgaagctt cgccgatctg
     7441 gcgcggctgg tcgtctgctt ctcgctgggc ttcactgtgt gggaatatct cggctcaccc
     7501 atcttcaacc attttggctg gggctattgg atctacacct tcatcgccga atcggtggcg
     7561 ctgatccttg ccggcctggt cgtcgcctgc tggttcatgc cgcatcacga gcggggggaa
     7621 gcgcattcaa cgccgcatcc ggagcaatcc gagtagcaac ccatcgtcgt cccagcgaag
     7681 gctgggaccc cggcgggtga aaggcgcaag cgg
//
LOCUS       DASWNU010000017         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_259990, whole genome shotgun sequence.
ACCESSION   DASWNU010000017 DASWNU010000000
VERSION     DASWNU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259990"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..2315)
                     /locus_tag="VGB57_00375"
     CDS             complement(96..2315)
                     /locus_tag="VGB57_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0129829.1"
                     /translation="MRTPQFEIHISVLAVAACLIAPGVAQAQTVPAQPGRPSPNAGPK
                     PGPAGDKPNEVVVTGTRSDVIATPDRVSFNVANDLQVQTGTVADALRAVPGVVVDPQG
                     RVSLRGDPGVTILIDGRPSTMLRGESRGDVLLSMPAGQIERVEVMTNPSAALSPEGSG
                     GVINLVTRQARKDTRSATVRATVGTEGRGALNLSGAHSGSGLTVTGDVGYRRMTGEAS
                     ATQLRSRVDPGSGAVVTSRQESELDNTNSARTARLGIEYDLDKKSRLGTELSYRDVSG
                     DVERSDSFVSQNAAFSYERGSEIDLFQRGVSARASWRRTLPGKEHEFSADLEVEKGLF
                     RREVEAVTVFASGQSFSEHIRNVGKRGETGVKLDYKKPLGTKGSLNLGYQGNFGDSEF
                     DYSGARGSALDALLPVPGLTNSFEYDQNIHAAFGTFRFPLGKLEVQSGLRLEQVETEI
                     RQVTDGLAFERDYFRAYPTLHLGYELSRTDQLRGSYSRRIQRPSPQDLNPYTFYIDPQ
                     NVRRGNPALLPEVTDSIEVGFQHRKAGNFYSLTGFYRRSRGGVTDILSDLGGGVFLTT
                     RANLATAQRAGVELIANGKLSKTLTYNASATFLWNEIDPRVGGLSASRSGTTGTARAN
                     LTWQPTKKDFFQLNASYAGKQLLPQGYRRSGGLLNLGYRRKVDDKLSVLLTGQNVLDS
                     ARQVTVFETPTLRDRFTQRGTGRVVLLGLVYNLGGQGGRKKPEPAFDFQQGGVDSPQ"
     gene            complement(2550..3095)
                     /locus_tag="VGB57_00380"
     CDS             complement(2550..3095)
                     /locus_tag="VGB57_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY0129830.1"
                     /translation="MSTPFFTIGHSTRTMEDFVELLRVGGVRMVIDIRTVPRSRTNPQ
                     YNLDSLGQALAGYQIGHGRIPALGGLRGRVREVPPERNGWWQNRSFQNYADHALSEDF
                     RAGLEELVALGRERPVAMMCSEAVWWRCHRRLIADHLIARGETVFHLMDRARAEPARL
                     SEGARVADGVVTYPGTPQPSS"
     gene            complement(3147..3587)
                     /locus_tag="VGB57_00385"
     CDS             complement(3147..3587)
                     /locus_tag="VGB57_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129831.1"
                     /translation="MLAGLGAGAVGAVAAAAPILSLRLSATGTGAGRPASWWDRTFLS
                     LQAAGLAEWSGVVGETFTLDSLNGSHMLRIVAVTAFPRSGPRPLELGRSQAFSVVFES
                     IAGPPLPATDRLCQLVHRSYPPLPIYMGAPFRVGQKSRLIAVFN"
     gene            complement(3857..>4522)
                     /locus_tag="VGB57_00390"
     CDS             complement(3857..>4522)
                     /locus_tag="VGB57_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0129832.1"
                     /translation="DYGPVPRDRGVRPNVWVRWGDDLILAREVRGQGAATELRKVSIY
                     DRDYETLKSIVTAERAVRAGNGWRLEQVETFDVARGIAQRSAATPFGEGLDPTQFTLA
                     SISADEQDFVTLRDSVRQLKMAGRPTAELESGLWHKLSGPLSAVLMPLLAGVAAFGLA
                     RSGQLFLRAVIGMFLGFAYFVADNFALAMGNLGAYPPLLAAWAPFALFALIGETVLIR
                     TEE"
BASE COUNT          744 a         1476 c         1550 g          752 t
ORIGIN      
        1 cgcgatccgc gcttccttcc ccttctaggc cgtatcgaca ttcagcctgg ccaattaacc
       61 cgctgaaggt cgatgcggcc caggctggcg ccgccttact gcgggctgtc caccccgccc
      121 tgctggaaat cgaaggccgg ctccggcttc ttgcgtcccc cctgcccgcc cagattgtag
      181 acgaggccga gaaggacgac ccggcccgtc cccctctggg tgaagcggtc gcgcagggtc
      241 ggggtctcga agacggtgac ctgcctggcg ctgtcgagga cgttctggcc ggtgagaagc
      301 acgctcaact tgtcgtctac cttgcggcga tagcccagat tgagaagtcc gcccgagcgg
      361 cgatagccct gcgggagcag ctgctttccg gcataggagg cgttcagctg gaagaagtcc
      421 ttcttcgtcg gctgccaggt cagattggcc cgggccgtcc cggtcgtgcc ggagcgcgag
      481 gcggagaggc cgcccacgcg cggatcgatc tcgttccaca ggaaggtggc cgaggcgttg
      541 taggtcaggg tcttgctcag cttgccgttg gcgatcagct cgacgccggc gcgctgcgcc
      601 gtcgccagat tggcgcgggt cgtcaggaag actccgccgc ccagatcgct gaggatgtcg
      661 gtcaccccgc ccctcgagcg ccggtagaat ccggtgaggg aatagaaatt gccggccttg
      721 cggtgctgaa agccgacctc gatcgaatcg gtgacctccg gcagcagggc gggattgccg
      781 cggcggacgt tctgggggtc gatgtagaag gtgtaagggt tgagatcctg cggcgacggg
      841 cgctggatcc gccggctgta gctgccgcgc aactggtcgg tcctggagag ctcgtagccg
      901 aggtgaagcg tcggataggc gcggaaatag tcgcgttcga aggccaggcc gtccgtgacc
      961 tggcggatct ccgtctcgac ctgctcgagc ctcaggcccg actggacttc gagcttgccg
     1021 agcgggaagc gaaaagtgcc gaaggcggcg tggatattct ggtcgtattc gaagctgttg
     1081 gtgaggccgg ggaccggcaa cagagcatcg agggccgagc cccgggcgcc ggaatagtcg
     1141 aactcgctgt cgccgaaatt gccctggtag ccgaggttca gagatccctt cgtgcccagc
     1201 ggcttcttgt agtcgagctt tacgccggtt tcgccgcgtt tgccgacgtt gcggatatgc
     1261 tccgagaaag actgtcccga ggcaaacacc gtgacggcct cgacctcgcg cctgaacagg
     1321 cctttttcga cctcgaggtc ggcggaaaat tcatgctcct tccccggcaa ggtccggcgc
     1381 cacgacgccc tcgcgctcac gccgcgctgg aacaggtcga tttccgagcc gcgctcgtag
     1441 gaaaaggcgg cgttctggct gacgaagctg tcggagcgct cgacgtcccc gctgacgtcg
     1501 cggtagctca gctcggtgcc gagccggctc ttcttgtcga gatcgtactc gatcccgagg
     1561 cgggcggtgc gggcggaatt ggtgttgtcg agctccgatt cctggcggct cgtcacgaca
     1621 gcccccgagc cggggtcgac ccgggagcga agctgggtag cgctcgcctc gcccgtcatc
     1681 cggcgatagc cgacgtcgcc cgtcaccgtg aggccggaac cggaatgggc gccgctcagg
     1741 ttgagagcgc cgcggccttc cgttccgacg gtggcccgga cggtcgccga gcgcgtatcc
     1801 ttgcgcgcct gcctggtcac caggttgatc actccgcccg acccttcggg gctgagcgcc
     1861 gcggacgggt tggtcatcac ctcgacccgt tcgatctgtc ccgccggcat cgacaggagg
     1921 acgtcgcctc gggattcccc gcgaagcatc gtggagggac gcccgtcgat caggatcgtc
     1981 acccccggat cgccgcgaag gctgacccgg ccctgggggt cgaccaccac gccagggacg
     2041 gcccgcaggg cgtcggcgac cgtcccggtc tggacctgca ggtcgttcgc gacattgaag
     2101 ctcacccgat ccggagtcgc gatgacgtcg gaacgcgtgc cggtgaccac cacctcattg
     2161 ggcttgtcgc cggcgggtcc gggcttcggc cccgcgttcg gggacggacg gccgggctgc
     2221 gccggaaccg tctgcgcctg tgcgacgccc ggcgcgatca ggcaggccgc aacggccagc
     2281 acggaaatgt ggatctcgaa ttggggggtt cgcatgcgtc ttgctgcctt catgaatgcc
     2341 tgatcgcctt cctgcctgag ggccaggcgt gcaggcgcga tgagccagcg gtggaattcc
     2401 gttgaaatac cggcggggaa ccgggccgat gccacgctgc cttccgatta tcgctccgga
     2461 ttccaatcgc atttgaccct ctgtattagg ttgctaaaac accaatacac ctaggtcaat
     2521 cttccggggt atgcagcgat tgcggacttt tacgacgaag gctggggcgt accgggatat
     2581 gtcaccacgc cgtcggccac acgggcgcct tccgagaggc gcgccggctc ggcccgcgcc
     2641 cggtccatca ggtggaaaac ggtctcgccg cgggcgatga gatggtccgc gatgagccgt
     2701 cggtggcagc gccaccagac ggcctcggag cacatcattg cgaccggccg ctcccgcccc
     2761 agagccacca gttcctccag ccccgcccgg aaatcctccg acagtgcgtg gtcggcataa
     2821 ttttggaagc tccggttctg ccaccagccg ttgcgttcgg gcggcacctc gcgcacccgg
     2881 cctcgaaggc cgccgagcgc cgggatccgg ccatggccga tctggtagcc ggccagcgcc
     2941 tggccgaggc tgtccagatt atattgcggg ttggtccgcg aacggggcac cgtgcggatg
     3001 tcgatcacca tccggactcc gccgactcga agcagctcga cgaaatcctc catggtccgg
     3061 gtcgaatggc cgatcgtgaa gaagggcgtg gacatgatag ccgggcaggt aacgcgatgc
     3121 cctcgctttg tctcccggcc ggccggctag ttgaacacgg cgatcaggcg gcttttctgc
     3181 ccgaccctga aaggcgcgcc catgtagatg ggcaagggcg gataggaacg atgtacgagc
     3241 tggcatagcc ggtcggtggc gggcagcggc ggcccggcga tcgattcgaa cacgaccgaa
     3301 aaggcttgcg agcggccgag ctccagcggg cgcggccccg atctggggaa ggcggtgacg
     3361 gcgacgatcc gaagcatgtg ggagccgttc aggctgtcca gggtgaacgt ctctccgacc
     3421 acgccgctcc attcggccag cccggcggcc tgaagcgaga ggaaagtgcg gtcccaccag
     3481 gaggccggcc ggcccgcgcc ggtaccggtg gctgacaggc gaaggctgag gatgggtgct
     3541 gccgccgcga cggcgccgac ggctccggca ccgagaccgg ccagcaacac gcgacggcta
     3601 aggaaagcgg aatcccgctg catcggtagc accccccaat ccaccgatga atgcccgccc
     3661 ccggacgcgc tccttgtaac gggaccgcgt gttcgatcaa gccggtgaaa tcaggacgtt
     3721 gcgtgttttt ttgggtcgct tccgcaagtg gcgcagaagt tcggaccttt ggccggccct
     3781 gggtcgggtt ttcagggccc gccggatcat ccgggctgtt cggcggggca gacggacggc
     3841 cgccattcgc cccgccttat tcctcggtcc ggatcagcac cgtctcgccg atcagggcga
     3901 acaaagcgaa aggggcccag gcggccagca gcggcgggta ggcgccgaga ttgcccatgg
     3961 cgagcgcgaa attgtcggcg acgaaatagg cgaagccgag gaacatgccg atgacggcgc
     4021 gaaggaagag ctgccccgag cgggcgagtc cgaacgcggc gactccggcc agaagcggca
     4081 tcagcaccgc cgaaagcggt cccgacaatt tgtgccagag cccggattcg agctccgccg
     4141 tcggtcggcc cgccatcttc agctgccgaa ccgaatcgcg aagggtgacg aaatcctgct
     4201 cgtcggcgga gatgctggcg agggtgaact gggtcggatc gagcccttcg ccgaatggcg
     4261 tggcggcgct gcgctgcgcg atgccccggg cgacgtcgaa cgtctcgacc tgctcgagcc
     4321 tccagccatt gccggcacgg accgcgcgct ccgcggtgac gatcgacttc agagtctcat
     4381 aatcgcgatc gtagatgctg accttgcgaa gctcggtcgc cgctccctgt ccacgcacct
     4441 cgcgggcgag gatgagatcg tcgccccagc gcacccagac gttgggccgg acgcctctgt
     4501 cgcgcggcac cggcccgtaa tc
//
LOCUS       DASWNU010000018         6129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_182213, whole genome shotgun sequence.
ACCESSION   DASWNU010000018 DASWNU010000000
VERSION     DASWNU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182213"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1861
                     /locus_tag="VGB57_00395"
     CDS             38..1861
                     /locus_tag="VGB57_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY0129833.1"
                     /translation="MNKFALLLAASLAGLSACTTTQAETQTATATQAPSGPQSEAGRL
                     KRLFHDSDEASLRRNPIGAIFRGDLRYADRLGDFITDEYFETERAAATADLAALRGID
                     RSRLSATDRIAYDVFEWQTLDSLKNLEPDMLALGVVRPINHFSGFHTFYPAFASGEGA
                     APFKTVADYENNLKRHKGYVVYHDRAIGRFREGIASGVFETRLTTRNVIEQLDLLIAQ
                     NVEESPFYAPVRNFPATFSEADKARLTAAYRTSIETEINPAHRRVRDFLRGEYLPKAR
                     EGVGLVHMKGGAKVYQRQIESTTTLPLTADEIHATGLREVARIKSEMEAVKARTGFKG
                     TLSQFFEYLRTDPKFAAVSRDALREGYEAIARRVEARIGEQFSTIPKSPLEIRAVEPF
                     REKTEAGGSYQQGTPDGSRPGIFYYNAYDLPSRRVWGMETLYLHEGSPGHHFQISLAQ
                     ENEALPAFMRFGGNTAFVEGWALYAETLWDELGLETDPYQRFGGLNDEMLRAMRLVVD
                     TGIHSKGWSREQAIDYMLANSGMSRTEVVAEVERYIAIPGQALAYKVGALTIQRLRAK
                     AEAALGQRFDPRAFHAQVLMTGSLPMAVLEKKIDDWIAATK"
     gene            2061..3881
                     /locus_tag="VGB57_00400"
     CDS             2061..3881
                     /locus_tag="VGB57_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY0129834.1"
                     /translation="MQPSYVLGAFTALLLASCASTDAPPPDATSAQAPRQSASEALAS
                     LFRESDEASLRRNPVQATARGDLRYADRLGDFFSDDYYQAEREAARADLAALAAIDRS
                     ALSADERVAYDVFKWQRGMDLRGLEGEIFAATAVRPVDHFNGFHTFFAELSSGQGIAP
                     YKNVKDYENGLARVDDFVELADSAIRRMEQGLAAGVVQPRLVMANVVEQLDAMLAEGV
                     EGSSFYRPVANFPAEVPEADRARLRAAYARSISTRIRPAVTRLRDFIRDRYLPKARPT
                     VGLQDMKGGPALYRHLVALSTTTDMTPDQIHRIGLAEVARLHKEMETVKAQVGFTGTL
                     QQFFEHIRTDPKFKPKSREALQQGYVAIGKRLDATLPTMFSTIPRTPLEIRPVPALTE
                     KGAARGSYQGGTVDGSRPGVFYFNAYDLPSRTTPGMETLYLHEGAPGHHFQISLAQEN
                     EALPSFMRFGGNTAYVEGWGLYAETLGRELGVYTDPYQYFGYLDSQLFRAIRLVVDTG
                     IHSKGWTRDRTIQYILDNSSRGRSNATAETERYIAIPGQALAYKIGQLKISELRARAQ
                     RELGPRFDIREFHAQVLMTGALPLAVLEKKIDDWIAAKKG"
     gene            complement(4096..5706)
                     /locus_tag="VGB57_00405"
     CDS             complement(4096..5706)
                     /locus_tag="VGB57_00405"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY0129835.1"
                     /translation="MPQSQVPEALRAAALVSRAWPYEEARKLLKRYPDGPPRGEIVFE
                     TGYGPSGLPHLGTFQEVARTLMVRHALSEMVDWPSRLIAFSDDMDGMRKVPPGVPGQD
                     MMHAHLDQPLSKVPDPYGCGHSSYAAHNNNLLLQFLDRFGFDYEFVASSNCYNSGRFD
                     EVLRQVLRKYRDILDIMLPTLREERRLTYSPVLPISPESGKVLQVPVEVVNAEAGLVR
                     YTDPESGQEVEQSILGGGAKLQWKVDWAARWVALGVDYEMSGKDHIDGVVQSSRIARA
                     LGARPPEGFNYELFLDENGEKISKTKGNGVTIDEWLSYAPRESLSFYIYREPKRAKQL
                     SVGVIPRAVDEYYQFLAAYPGQEWDKRLGNPVHHIHAGKVPDVRLPISFALLLNIASL
                     PGVGDRETVWSFLHRYNPALTPEASPELDRLVSYAVEYGCRFVAPALRRRPPTPEEVP
                     ALRELDELLARDDGTGREDEDPAVLLQNHVYEIGKKYYGKERLRDWFKALYETLLGSE
                     QGPRMGSFIALYGVENSRRLIAEALESA"
     gene            5761..>6129
                     /locus_tag="VGB57_00410"
     CDS             5761..>6129
                     /locus_tag="VGB57_00410"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0129836.1"
                     /translation="MTVIQPINVPQRVKLTIEQFELLDRSGAFDGYAKTELIEGAIYA
                     MEGQFRPHAFAKSELYFRLRTALDAINSPLMPIVEGSVAMPPLSAPEPDICVTAAPRG
                     EGYIPLDSVALAVEVSDSTAA"
BASE COUNT         1086 a         2086 c         1970 g          987 t
ORIGIN      
        1 gcttccccct ctctccgccg cccgctaagc aacggccatg aacaaattcg cgctcctgct
       61 cgccgcctcg ctcgccggcc tttccgcctg caccaccacc caggccgaaa cgcagaccgc
      121 cacagcgacc caagcccctt ccgggccgca gagcgaggcc gggaggctga agcggctgtt
      181 ccacgacagc gacgaggcca gcctcaggcg caatccgatc ggcgccatct tccggggcga
      241 cctgcgctac gccgaccggc tcggcgattt catcaccgac gaatatttcg agaccgagcg
      301 ggcggccgcg acggccgacc tcgccgccct gcgcgggatc gaccggtcca ggctgagcgc
      361 caccgaccgg atagcctatg acgtcttcga atggcagacc ctggattccc tgaagaatct
      421 cgagcccgac atgctcgcgc tcggagtggt ccgaccgatc aaccatttct ccggcttcca
      481 caccttctat cccgctttcg cctcgggcga gggggcggcg ccgttcaaga cggtggcgga
      541 ttatgagaac aatctgaagc gccacaaagg ctatgtcgtc tatcacgacc gcgccatcgg
      601 acgcttccgg gagggcatcg cctccggcgt gttcgagacc aggctgacga cccgcaacgt
      661 gatcgagcag cttgatttgc tgatcgccca aaatgtggag gaatcgccat tttatgcacc
      721 ggttcgcaac tttccggcca cattttcgga ggccgacaaa gcgcgcctga cggccgccta
      781 ccggacgtcg atcgaaaccg aaatcaatcc ggcccaccgc cgggtccgcg acttcctgcg
      841 cggcgaatat ctgcccaagg cgcgcgaagg ggtcgggctc gtccacatga agggcggggc
      901 caaggtctac cagcggcaga tcgagagcac gacgaccctg ccgctcaccg ccgacgagat
      961 ccacgctacc gggctgcgcg aggtcgcccg gatcaagagc gagatggagg cggtaaaggc
     1021 gcgcaccggc ttcaagggca cgctctccca gttcttcgaa tatcttcgca ccgatcccaa
     1081 attcgccgcc gtctccaggg acgcgcttcg cgaaggctat gaggcgatcg cccgccgggt
     1141 cgaagcgcgg atcggtgagc aattctcgac catcccgaag agcccgctcg agatccgagc
     1201 cgtcgagccc ttccgggaga agaccgaggc cggcggctcc taccagcagg ggacgcccga
     1261 cgggtcgcgg ccgggaatct tctattacaa cgcttacgac ctgccctcgc gccgggtctg
     1321 gggcatggag acgctctacc tccacgaagg cagccccggc caccatttcc agatcagcct
     1381 cgcccaggaa aatgaggcgc ttcccgcctt catgcgcttc ggcggcaaca ccgccttcgt
     1441 cgagggctgg gccctctatg ccgagacctt gtgggacgag ctcggcctcg agaccgatcc
     1501 ctaccagcgc ttcggcggcc tcaacgacga gatgctccga gccatgcggc tcgtcgtcga
     1561 caccgggatc cattcgaaag gctggagccg cgagcaggcg atcgactaca tgctcgccaa
     1621 cagcgggatg agccggaccg aggtcgtcgc cgaggtcgag cgctacatcg ccattccggg
     1681 ccaggcgctg gcgtataagg tgggggcgct caccatccag cgcctccggg ccaaggcgga
     1741 ggcggcgctt gggcagcggt tcgacccgcg cgcgttccac gcccaggtgc tgatgacggg
     1801 atcgctgccg atggcggtgc tggagaagaa gatcgacgac tggatcgcgg cgacgaaata
     1861 gattgcctcc ccatcgccag atggggaggg ggaccatgcg aagcatggtg gaggggtaga
     1921 tggcccggaa gttctgggtt cgatcgagaa cttcacctgc ggagccaatt acccctccac
     1981 cacccttcgg gtggtccccc tccccacccg ctccgcgggc agggaggcaa atccggcgcg
     2041 acgcctgaca ggagaagaca atgcaaccat cgtacgtgct gggcgctttc acggccctcc
     2101 tccttgcgag ctgcgcttcc accgacgcac cgccacccga cgccacaagc gcccaggctc
     2161 ctcggcaatc cgcctccgag gctctcgcct ccctgttccg cgagagcgac gaggcgagcc
     2221 tccgccgcaa ccccgtccag gccaccgcgc gcggcgacct gcgctacgcg gaccgtctcg
     2281 gcgacttctt cagcgacgat tattaccagg cggagcggga ggcggcacgg gcggacctgg
     2341 cggcgctggc cgcgatcgac cggagtgcgc tgagcgccga cgagcgggtc gcctacgacg
     2401 tcttcaaatg gcaacggggc atggacctgc gcgggcttga gggcgagatc ttcgccgcca
     2461 cggcggttcg gccggtcgac catttcaacg gcttccacac cttcttcgcc gagctcagct
     2521 cgggccaggg catcgccccg tacaagaatg tgaaggatta tgagaacggg ctcgcccggg
     2581 tcgacgattt cgtcgagctg gccgacagcg ccatccgccg gatggagcag gggctcgcgg
     2641 cgggcgtggt gcagccaagg ctggtcatgg ccaatgtcgt cgagcagctc gacgcgatgc
     2701 tggccgaagg cgtggaagga agcagcttct accgtcccgt cgccaatttc ccagcggagg
     2761 tgcccgaggc ggaccgggcg cgcctgaggg ccgcctacgc ccggtcgatc tcgaccagga
     2821 tccggccggc ggtgacccgg cttcgcgatt tcatccgcga ccgctacctg ccgaaggcgc
     2881 gcccgaccgt cgggcttcag gacatgaagg gcggccccgc cctctaccgc catctggtcg
     2941 cgctgagcac caccaccgac atgacccccg accagataca ccggatcggc ctcgccgagg
     3001 tcgcccggct ccacaaggag atggagacgg tgaaagccca ggtcggcttc actgggacgc
     3061 tccagcaatt tttcgagcac atccgcaccg atccgaagtt caagccgaag tcgcgcgaag
     3121 cgcttcagca gggctatgtc gcgatcggca agcggctgga cgcgaccctg ccgacgatgt
     3181 tctcgaccat ccccaggacc ccgctcgaga tccggccggt cccggccttg accgagaaag
     3241 gggcggcgcg cggctcctac cagggcggca ccgtcgacgg ctcgcggccg ggagtcttct
     3301 atttcaacgc ctacgatctg ccctcgcgca ccacccccgg gatggagacc ctctatctcc
     3361 acgaaggcgc gcccggccac catttccaga tcagcctcgc ccaggagaat gaggctcttc
     3421 cgagcttcat gcgcttcggc ggcaacaccg cctatgtcga gggctggggc ctttacgcgg
     3481 agaccctggg gcgggagctg ggggtctata ccgaccccta tcaatatttc ggctatctcg
     3541 attcgcagct gttccgggcg atccggctgg tcgtcgacac cggcatccat tcgaaaggct
     3601 ggacccgcga ccggaccatc cagtacatcc tcgacaacag ctcgcgcgga cgaagcaacg
     3661 ccaccgccga gaccgagcgc tacatcgcca tcccgggcca ggcgctcgcc tacaagatcg
     3721 gccagctcaa gatcagcgag ctgcgggccc gcgcgcaacg ggagctgggg ccccgcttcg
     3781 acatccgcga gttccacgcg caggtgctga tgaccggcgc actgccgctc gccgtgctcg
     3841 agaagaagat cgacgactgg atcgcggcga agaagggctg agaggtccgc cgggcacgtc
     3901 cacgacccgt catcccggcg gaggccggga cctcggggag acccaccggg acttcttgtc
     3961 ctgaggtccc ggctttcgcc gggatgacga gaggggctgc ttgcgtcatc ccagcgaagg
     4021 ctgggattcc ggcgggtgaa aggcgccggc ggtcttcacc cgccggggcc ccagccttcg
     4081 ctggggcgac ggggatcagg ccgactccag cgcctcggcg atcagccttc ggctgttctc
     4141 gactccatag agggcgatga agctgcccat gcgggggccc tgctcgcttc cgagcagggt
     4201 ctcgtaaagc gccttgaacc agtcgcgcag gcgctccttg ccgtaatatt tcttgccgat
     4261 ctcgtagacg tggttctgca ggaggacggc cggatcctcg tcctcgcggc cggtgccgtc
     4321 gtcccgggcg agcagctcgt ccagctcccg cagcgccggc acctcctcgg gggtgggcgg
     4381 ccgccgccga agcgcgggcg cgacgaagcg gcagccatat tcgaccgcat aagagacgag
     4441 cctgtcgagc tcggggctcg cctccggagt cagagcggga ttgtagcggt ggaggaagct
     4501 ccacaccgtc tcccgatcgc ccacgccggg aaggctggcg atgttgagga gcaaggcgaa
     4561 gctgatcgga agcctcacgt cgggcacctt gccggcatgg atgtggtgga cgggattgcc
     4621 gaggcgcttg tcccactcct gccccggata ggcggcgagg aactggtaat attcgtcgac
     4681 cgccctcggg atgaccccga cgctgagctg cttcgcccgc ttcggctcgc ggtagatgta
     4741 gaaagagagg ctctcccggg gcgcgtagct cagccactcg tcgatcgtga ctccattgcc
     4801 cttggtcttc gagatcttct cgccattctc gtcgaggaac agctcgtaat tgaacccttc
     4861 gggaggccgg gcgccgagcg cgcgcgcgat cctcgagctt tgcacgactc cgtctatgtg
     4921 gtccttgccg gacatctcat aatcgacgcc gagcgctacc cagcgagccg cccagtcgac
     4981 cttccactga agcttggcgc cgccgccgag gatcgactgc tcgacctcct ggccgctctc
     5041 cgggtcggtg tagcggacca gccccgcctc ggcattcacc acctcgaccg ggacctggag
     5101 caccttgccg ctttcggggc tgatcggaag caccggcgaa taggtgagcc gccgctcctc
     5161 gcgaagcgtc ggaagcatga tgtcgagaat gtcccgatac ttgcgaagca cctgccgaag
     5221 cacctcatcg aagcggccgg agttgtagca gttcgaggag gcgacgaact catagtcgaa
     5281 gccgaagcgg tcgaggaatt gcaggagcag attgttgttg tgcgcggcat agctggagtg
     5341 gccgcagcca taggggtccg gcaccttgct cagcggctgg tcgagatggg catgcatcat
     5401 gtcctgcccg ggaacgccgg gcggaacctt gcgcatcccg tccatgtcgt ccgaaaaagc
     5461 gatcagccgg ctaggccagt cgaccatctc cgacaaggcg tgacggacca tcagggtgcg
     5521 cgccacctcc tggaaggtgc ccagatgcgg caggccgctc gggccgtagc cggtctcgaa
     5581 cacgatctcg ccgcggggcg gcccgtcggg gtagcgcttc agcagcttgc gcgcttcctc
     5641 atagggccat gccctggaga cgagcgcggc ggcgcggagg gcttcgggaa cttgagactg
     5701 gggcatgaac cgtctcatcg ggatttcgtt tgtgtcgcgc aagtgtggta agaaactccc
     5761 gtgaccgtca tccagccgat caacgtaccc caacgggtga aactcaccat cgagcagttc
     5821 gagctgctcg acagatcggg cgcgttcgac ggttacgcaa agaccgagct gatcgaggga
     5881 gcgatctacg cgatggaggg gcagttccgt ccgcacgcct tcgcgaagag cgaactctat
     5941 tttcggctga ggacggcgct cgatgccatc aactcgccgc tgatgccaat tgtcgaaggc
     6001 tcggtcgcca tgccgccgct cagtgcgccg gaaccggaca tttgcgtcac ggccgctcca
     6061 cgcggggaag gctatatccc gctcgattca gtcgccttgg ccgtcgaggt ttcggattcc
     6121 accgcggcg
//
LOCUS       DASWNU010000019         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_182214, whole genome shotgun sequence.
ACCESSION   DASWNU010000019 DASWNU010000000
VERSION     DASWNU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182214"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VGB57_00415"
     CDS             <1..322
                     /locus_tag="VGB57_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEY0129837.1"
                     /translation="FGYLSDKGETSIRLAGYYNQPLTGALILQPSAELDYYGEDVPEL
                     GLGRGFGYAEAGLRLRYEVKEAFAPYVGLSWSRDLGRTARLTRDEGEDPETRSVVMGV
                     RSAF"
     gene            complement(412..684)
                     /locus_tag="VGB57_00420"
     CDS             complement(412..684)
                     /locus_tag="VGB57_00420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0129838.1"
                     /translation="MAEVTNNEAASQYELEVEGQLALAAYRLRDGRITFTHTEVPDAL
                     AGQGIGKRLIKAALDDVREQGLKVVPACAFVKHYIDTHPETKDLLA"
     gene            complement(800..1834)
                     /locus_tag="VGB57_00425"
     CDS             complement(800..1834)
                     /locus_tag="VGB57_00425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0129839.1"
                     /translation="MRFGYWMPVFGGWLRNIEDEGMEASWDYVRRLTLRSEEAGWDLS
                     LIAELNLNDIKGVEAPALDAWSTAAALAAVTERIELMVAVRPNFHQPALFAKQAANID
                     NISGGRLSLNVVSSWWADEATQYGLQFDQHDDRYARTREWLDVVDGLWTEKRFTYEGE
                     RYRLKDAICEPKPVSSPRPTIYAGGESEAAKTMISDQCDAYVIHGDPVEAVAPKIADM
                     AARRERAGKPEMQYGMAAYAIVRDSEAEAKRELERITALPPRPPKGFDNFDQWLSGTQ
                     LERELKLQEYSVSNRGLRPNFVGTPEQLRERIEEYEAAGLDLLLLQMSPQAEEMDRFA
                     AQVIAPMKAA"
     gene            complement(1834..2754)
                     /locus_tag="VGB57_00430"
     CDS             complement(1834..2754)
                     /locus_tag="VGB57_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HEY0129840.1"
                     /translation="MKPDLAILFEHPTWFEPLFAALDRRGLSYEAVRLSDHSFDPASP
                     ETPAPVILSRVAMSSFLREAEHGIFYAEALLAHWAANGARVLNGAEVIAVDSSKARQL
                     SLISRLGHDVPETRVVHRRRDLLAAAGTMRWPLLVKANIGGSGAGIVRYGSEDELAAS
                     MAEGSVPESVDSVLLVQDYVPARGGTIVRAETLGGKFLYAIEVESGGDSFDLCPADAC
                     LAQPGRAAVRMTAVTPPPELIEAAERIAQAAGLDVGGVEYLIDDRDGIARFYDINALS
                     NFVAKPLDVLGWDPHERLVDHLETAIRESR"
     gene            complement(2896..4866)
                     /locus_tag="VGB57_00435"
     CDS             complement(2896..4866)
                     /locus_tag="VGB57_00435"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0129841.1"
                     /translation="MADFAAQREHMVRQQIAGRGIEGARLLDAFRAVPREDFVPEGVR
                     EFAYEDAPLPIEAGQTISQPYIVALMIAAAEVAPGDRVLEVGAGSGYAAAVMSRLAGT
                     VIAVERHPELAVLARARMERLGYRNVRILEGDGTGGLPGEAPFEAILCAASGSHVPDA
                     LRRQLSIGGSLVMPLGEPNAAQKLVKVTRRGEEEFEQEDLGPVRFVPLIGAHGWADPK
                     GEKTMSVPELIAAAAEPLPGPEEPAFAGAFDRFGEARVVLLGEASHGTSEFYRARAAI
                     TRRLIERHGFNIVAVEADWPDAASIDRYVRHRPRREGEEAAFQRFPTWMWRNFEVDAF
                     VRWLRAHNGGRPHEAMAGFYGLDLYNLGASIRAVIDFLDEADPAAAKVARERYGCLRP
                     WAHDPAAYGRVAITEGYGRCEVGVVQMLRELMTRRMDCLSPECDEWLDAAANARLIKN
                     AEQYYRVMYYGSAESWNLRDTHMFETLCQLLEAKGPDSKAVVWAHNSHIGNAAHTEMG
                     QVREELNIGQLVKERFGDRARLIGFGTHTGTVAAATDWDEPMEVKNVRPSLPESYERL
                     CHDSAVPRFLLDLRDGVNEAAAEALLEPRLERFIGVIYRPETERWSHYAQAVLPNQFD
                     AWVWFDETEAVTPLPGAEAPPAGEDETYPFGL"
BASE COUNT          751 a         1788 c         1656 g          832 t
ORIGIN      
        1 cttcggctat ctctccgaca agggcgagac cagcatcagg ctggccggct attacaacca
       61 gccgttgacc ggcgccctca tcctccagcc cagcgccgag ctcgattatt atggcgagga
      121 cgtgccggag ctcggcctcg gccgcggctt cggctatgcc gaggcgggcc tgcgcctgcg
      181 ctacgaggtc aaggaagcct tcgcgcccta tgtcggcctc agctggtccc gcgacctcgg
      241 ccgaaccgcc cgcctcaccc gcgacgaagg cgaggatccg gagacgagga gcgtcgttat
      301 gggggtgcgc tcggcctttt gatccccccg tcgtcccggc gaaagccggg gcctcgggga
      361 gacccgccga gatttctcga catggggtcc cggctttcgc cgggatgacg gctacgccag
      421 caggtcctta gtctccgggt gagtgtcgat ataatgcttc acgaacgcgc aagcgggcac
      481 gaccttcagc ccctgctccc ggacgtcgtc cagcgcggcc ttgatcagcc tcttgccgat
      541 cccctgcccc gccagtgcgt ccggcacctc ggtgtgggtg aaggtgatcc gcccgtcccg
      601 aagccgatag gccgcgagcg cgagctgtcc ctcgacctcc agctcatact ggctcgccgc
      661 ttcgttgttc gtcacttcgg ccatcgatca ctccttactc tttcccgtca ttgcgaggag
      721 cgcagcgacg aagcaatcca gctacggagc ccccaacgaa cgcggtcctg gattgcttcg
      781 ctgcgctcgc aatgacggct caggccgcct tcataggcgc gatgacctgc gcggcgaagc
      841 ggtccatctc ttccgcctgc gggctcatct gaagcagaag caggtcgagc cccgccgcct
      901 catattcctc aatccgctcg cgcagctgct cgggggtgcc gacgaaattg ggcctgaggc
      961 cgcggttcga gaccgaatat tcctggagct tcagttcgcg ctcgagttgg gtgccggaca
     1021 gccactggtc gaaattgtcg aagcctttag gcggccgggg cggcaacgcg gtgatccgct
     1081 ccagctccct tttcgcctcg gcctcgctgt cgcggacgat cgcataggcg gccattccgt
     1141 actgcatttc cggcttgccg gcccgctcgc gccgggccgc catgtccgcg atcttcggag
     1201 ccaccgcctc gaccggatcg ccgtgaatga cataggcgtc gcactggtcc gagatcatcg
     1261 tcttggcggc ctcgctttcg ccgccggcgt agatcgtcgg gcgggggctg ctcaccggct
     1321 tgggctcgca gatcgcgtcc ttcagccggt agcgctcgcc ttcatacgtg aagcgcttct
     1381 cggtccacag cccgtcgacc acgtcgagcc attcccgggt ccgggcgtag cgatcgtcat
     1441 gctggtcgaa ctggagccca tattgggtcg cctcgtcggc ccaccaggag gagacgacgt
     1501 tgagcgacag ccgcccgccg ctgatattgt cgatgttcgc ggcctgcttg gcgaacaggg
     1561 ccggctggtg gaaattgggc ctgaccgcga ccatcagctc aatccgctcg gtcaccgccg
     1621 ccagcgccgc cgccgtcgac caggcgtcga gcgcgggcgc ctccacgccc ttgatgtcgt
     1681 tcaggttcaa ttcggcgatc aggctcagat cccagccggc ctcctcgctt cgcagggtga
     1741 ggcggcggac atagtcccag ctcgcctcca tgccctcgtc ctcgatgttc ctgagccagc
     1801 cgccgaagac cggcatccaa taaccgaacc gcatcatctg ctctcccgga tcgccgtttc
     1861 gaggtgatcg acgagccgct cgtgcgggtc ccagcccagc acgtccagcg gcttcgcgac
     1921 gaaattcgac agcgcgttga tgtcgtagaa gcgggcgatg ccgtcgcggt cgtcgatcag
     1981 atattcgacc ccgccgacat cgagcccggc cgcttgcgcg atccgctccg cggcttcgat
     2041 cagctccggc gggggcgtca cggccgtcat ccgcaccgcc gcccggccag gctgggccag
     2101 gcaggcgtcg gccgggcaca ggtcgaagct gtcgccgccg ctctccacct cgatggcgta
     2161 gaggaacttg ccgcccaaag tctccgcccg gacgatcgtc ccgccgcgcg ccgggacgta
     2221 atcctggacg aggagaacgg aatcgacgct ttccggaacg ctcccctcgg ccattgaagc
     2281 cgccagctcg tcctcgctgc cgtagcgcac gatgccggcg cccgagccgc cgatattggc
     2341 cttgaccagc agcggccagc gcatcgtccc ggccgcggcg agcaggtccc ggcggcggtg
     2401 gacgacccgg gtctccggca cgtcgtggcc gagccgggag atgagcgaga gctgccgcgc
     2461 cttggaggaa tcgacggcga tgacctccgc cccgttcagc acccgcgccc cgttcgcggc
     2521 ccagtgggcg agcagcgcct cggcatagaa gatgccgtgc tctgcttcgc gaaggaagct
     2581 cgacatcgcg acccggctca ggatcacggg ggcgggggtt tcgggtgagg ccggatcgaa
     2641 gctgtggtcg gacagccgga cggcctcata gctgaggccg cgccggtcga gcgcggcgaa
     2701 cagcggctcg aaccaggtcg ggtgctcgaa gaggatggcg agatcgggtt tcatgcgtga
     2761 ggcacttggg ggcgggaggg gcggatgtga aggcttcgtc cgtcatcccg gcgaaagccg
     2821 ggacctcagg acgagcagtc tccacgggct ccacgaggtc ccggctttcg ccgggatgac
     2881 gttcttcccc catcgtcaca gcccgaacgg gtaggtctcg tcctctcccg ccggcggcgc
     2941 ctccgcgccc ggtagcggag tcaccgcctc ggtctcgtcg aaccagaccc aggcatcgaa
     3001 ctggttgggc aggacggcct gcgcataatg gctccagcgc tcggtctccg gccggtagat
     3061 gactccgatg aatcgctcca gccgcggctc gagcaaagcc tccgccgccg cctcgttcac
     3121 gccgtcgcga aggtcgagga ggaagcgggg gactgcgctg tcgtggcaca gccgctcgta
     3181 gctctcggga agcgagggcc ggacattctt cacctccatc ggctcgtccc agtcggtcgc
     3241 cgcggcgacg gtgcccgtat gcgtgccgaa gccgatcagc cgcgcccgat cgccgaacct
     3301 ttccttgacc agttggccga tgttgagctc ctcgcgcacc tggcccatct cggtatgcgc
     3361 ggcattgccg atatggctgt tatgggccca gaccaccgcc ttggaatccg gccccttcgc
     3421 ctcgagcagc tggcacagcg tctcgaacat gtgggtgtcg cgcagattcc agctttccgc
     3481 cgagccgtaa tacatgaccc ggtaatattg ctcggcattc ttgatcagcc gcgcgttggc
     3541 ggcggcgtcg agccattcgt cgcattcggg cgaaaggcag tccatccgcc gggtcatcag
     3601 ctccctgagc atctggacga cgcccacctc gcaccgccca tagccttcgg tgatcgcgac
     3661 ccgcccgtag gcggccggat cgtgggccca gggccgaagg cagccatagc gctcgcgcgc
     3721 gaccttggct gcggcggggt ccgcttcgtc gaggaagtcg atcacggccc ggatcgaggc
     3781 tccgagattg tagaggtcga ggccgtagaa gccggccatc gcctcgtgag gccggccgcc
     3841 gttatgcgcc ctcagccagc gaacgaaggc gtcgacctcg aaattgcgcc acatccaggt
     3901 cgggaagcgc tggaaggccg cctcctcccc ctcccggcgg ggccggtggc ggacgtagcg
     3961 gtcgatgctg gcggcgtcgg gccagtcggc ctcgaccgcg acgatgttga agccgtggcg
     4021 ctcgatcagc cgccgggtga tcgccgcccg cgcccggtag aattccgacg tgccgtgact
     4081 ggcctcgccg aggagcacca cccgcgcctc cccgaaccgg tcgaaagcgc cggcgaaggc
     4141 cggttcctcc ggtccgggca gcggctcggc cgcggcggcg atcagctctg gcaccgacat
     4201 ggtcttctct cctttgggat cggcccagcc atgggccccg atcaggggca cgaagcgcac
     4261 cggcccgaga tcctcctgct cgaattcctc ctcgccgcgg cgagtgacct tgacgagctt
     4321 ctgggcggcg ttcggctccc cgagcggcat caccaggctt ccgccgatcg acagctgacg
     4381 gcgaagcgca tcgggcacgt ggctgccgct cgccgcgcat aatatcgcct cgaatggagc
     4441 ttcgccggga aggcccccgg tgccgtcgcc ctccaggatc cggacattgc ggtaaccgag
     4501 ccgctccatc cgcgcccgcg ccagcacggc cagctcgggg tggcgctcga ccgcgatcac
     4561 cgtcccggcg aggcggctca tcacggcggc cgcgtagccc gagccggcgc cgacctcgag
     4621 caccctgtcg ccgggcgcga cttcggccgc ggcgatcatc agcgcgacga tatagggttg
     4681 cgagatggtc tgccccgcct cgatcggaag cggcgcgtcc tcgtaggcga attcgcgcac
     4741 gccctcgggc acgaagtcct cgcgcggcac cgccctgaag gcgtcgagga gccgcgctcc
     4801 ctcgattccg cggccggcga tctgctgccg gaccatatgc tcgcgctggg cggcgaagtc
     4861 cgccatctga gcctcctgtc gcacccataa ccggcgcggc gggagcgtgt tcctaaccgc
     4921 cgcacccgat ggccggcgcc ggcgcggcgg ctagggcgcc gggctttggg ccggcggacg
     4981 ggctccgccg gcgcgctgac ggacgacccg gatataggag tcgacga
//
LOCUS       DASWNU010000020         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_363678, whole genome shotgun sequence.
ACCESSION   DASWNU010000020 DASWNU010000000
VERSION     DASWNU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363678"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VGB57_00440"
     CDS             complement(<1..1366)
                     /locus_tag="VGB57_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129842.1"
                     /translation="MANVRSAISVTIALALLLALAPAVAAKGVPVVHLVVLPAGTTPD
                     ELAEAGFSPGLMSAGLGAVPRNQTYLDIGQGNRVFDSLYDQSLPWSQGCGSWQKEVEV
                     RAESAPAEIVPFLLVRKARRHGLTVSWTGRLACAFGGNRRIDPGNRGALFFLEGALLG
                     KAGEMRRFLESDLLIVIAEPEGESDEPVAIGIAGRGFDGNLTSGSTRTDGYVLSTDVA
                     PTILDRLGVEVPSEMSGQPIRAEGAVDAAAIESLGARMEVISSRRGTAVGLPIAAWLL
                     LAALIALVSRGALARPIVRVLGLSVVYLPLLLLLGATLEPAETAEALLVGLGAPLLGI
                     LTLALLGGYRALAVASALTVLAYSIDVVAGSPLTSLSLLGPNPGLGVRFYGIGNELEA
                     LLAVLGVAGVGAGLTAFAPRSSQRAKAAAFVFAGLLAAFVFAAGRFGADVGAAIVFPI
                     GAAVA"
     gene            1344..>1604
                     /locus_tag="VGB57_00445"
     CDS             1344..>1604
                     /locus_tag="VGB57_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129843.1"
                     /translation="MADRTFAKFTFFKIDPAWRRRDPELRAADKREFLAACEDFDVDR
                     SLRAYSTIGTRGDVDLVLLSQSPNLNDIHTFHVVLGQSGLAKW"
BASE COUNT          278 a          583 c          539 g          204 t
ORIGIN      
        1 ctgccaccgc ggcgccgatc gggaagacga tcgccgcgcc tacgtcggcg ccgaagcggc
       61 cggcggcgaa gacgaaggcg gcgagaagcc cggcgaagac gaatgcggcc gccttcgccc
      121 gctgggagga ccgcggcgcg aaggcggtca gcccggcgcc gacgccggcc acccccagca
      181 ccgcgagcag ggcctcgagc tcgttgccga tcccgtagaa gcgcaccccg aggccggggt
      241 tcggccccaa cagcgagagc gacgtcaggg gcgagccggc gacgacgtcg atcgagtagg
      301 cgaggacggt gagggccgag gccaccgcga gggcccggta cccgcccaga agcgccagcg
      361 tcaagatccc cagcaaaggc gcccccagcc ccaccagcag cgcttcggcc gtctcggcag
      421 gctcgagtgt cgcccccagc aggagcagca acggcaggta gacgacgctc agacccaaga
      481 ctcggacgat cggccgcgct agcgcccccc ggctgaccag ggcgatcagt gcggccagca
      541 gcagccaggc tgcaatcggc aggccgaccg cggttccgcg tcgcgaggag atcacctcca
      601 tccgcgcccc cagcgattcg atcgccgccg cgtcgacggc tccctcggcg cggatcggct
      661 gccctgacat ctcgctcggc acctccaccc cgagccggtc gaggatcgtc ggcgccacat
      721 ccgtggagag gacgtagccg tccgtgcggg tgctgccgga ggtcaggttg ccgtcgaagc
      781 ctcggccggc gatccctatc gccaccggct cgtctgactc gccttcaggt tcggcgatca
      841 cgatcagcag gtcggattcg agaaaacggc gcatctcccc ggcctttccc agcagcgcac
      901 cttcgagaaa gaacaacgcg cccctgttcc cgggatcgat ccgcctgttc cctccgaagg
      961 cgcaggcgag cctccccgtc caggaaacgg tgaggccgtg gcgccgagct ttgcggacga
     1021 ggaggaaggg gacgatctcc gccggcgccg attcggctcg cacctcgacc tccttctgcc
     1081 agctcccgca gccctggctc cacggcaggc tctggtcgta cagggagtcg aagacccggt
     1141 tgccctgacc gatgtcgagg taggtctggt tgcgggggac ggcgccgaga ccggcgctca
     1201 tcaggccggg cgagaagccc gcctcggcca gctcgtcggg cgtcgttccc gcaggaagca
     1261 cgacgagatg gacgaccggg acacccttcg ccgccaccgc gggcgccaga gccagcagca
     1321 gggcgagcgc tatcgtcacc gagatggccg accgcacgtt cgccaagttc acgtttttca
     1381 agatcgatcc agcctggcgt cgtcgggacc cggagcttcg ggcagccgac aagcgggaat
     1441 tcctcgccgc ctgcgaggac ttcgacgtcg accgctcctt gcgtgcctac tcaaccatag
     1501 gaacgcgggg cgacgtcgac ctggtgctgc tgagccaaag cccgaacctc aacgacatcc
     1561 acacctttca cgtcgtgctc ggccagagcg ggctggcgaa atgg
//
LOCUS       DASWNU010000021         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_389714, whole genome shotgun sequence.
ACCESSION   DASWNU010000021 DASWNU010000000
VERSION     DASWNU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389714"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VGB57_00450"
     CDS             <1..469
                     /locus_tag="VGB57_00450"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY0129844.1"
                     /translation="AYVLIKILSPGFYARRDIKTPVFVAVATLLGSVVLNFILVPRLG
                     IVALPLSTAIGAWANAFLLAALLHARGHFRLPGWLASRLVRQLLAGAAMGVTLWLIER
                     ALADRFAGSAGDRVLAMGALVAAGGIVYFAVAWTIGAMNREDILILLRRKKVD"
     gene            601..1272
                     /locus_tag="VGB57_00455"
     CDS             601..1272
                     /locus_tag="VGB57_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY0129845.1"
                     /translation="MSLAKKLLTLAAPAALLALGGCATGLPTQVSRYQAMPAPAGQSF
                     AIQAADPEDRGGLEFSRYAELVRRHMIAQGYSEAASPQQASLIVSLDYGVDDGRQQVV
                     TYPSSRFGSRFGYGGFGYRPYWSRFGGYGRLRSPFYYGWDDPFWYRGFGEEIDVYTVY
                     SSFLDMDIRRPDGQAVFEGTARARSRTDELSVLVPNLIEAMFTGFPGNSGETVKITVA
                     PERRR"
     gene            1489..2430
                     /locus_tag="VGB57_00460"
     CDS             1489..2430
                     /locus_tag="VGB57_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY0129846.1"
                     /translation="MLKYKGAAAMAAIALASAAPAQNKAADRAQVEDIVVTGRKSGVP
                     MWIVRGDTTTLVLVGGINGVSKTTHWDPAALTEALRKADRVMFPQSYALTASPFRAIG
                     WIAKWNKMGSLPKNQSLGAIAGPQAMRRLAALRARNMVRADYDRRHPLHLSQELRSRA
                     KGDIDYSRDVAEYARNAAKKHKLAEVVPIQRSKAKPVVKDLFASTPQEHVPCLEAALT
                     MAEAGPAAVQARSDAWAARRVRDVLSSPADPVYERCWPSGVFADREVELLPQMKQLLA
                     EPQVTVAVLSLRTLAEQGGLLDGLAAAGFDIQGPAWK"
     gene            complement(2539..2946)
                     /locus_tag="VGB57_00465"
     CDS             complement(2539..2946)
                     /locus_tag="VGB57_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0129847.1"
                     /translation="MGGSLIALQAPTNALLARGVGSAVNAAFVSFVVGTIALLAVAAA
                     LGARPAAGVTGALPWYAWVGGFYGAFFVAVAAFAAPRMGVTFFLMVAIAGQLAMALFL
                     DRIGAFGLARVEIGPTRIAGILLVLAGAYLVRR"
     gene            complement(3027..>3453)
                     /locus_tag="VGB57_00470"
     CDS             complement(3027..>3453)
                     /locus_tag="VGB57_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0129848.1"
                     /translation="KRINSNVRELEGALNRIAAYATMQNRVIDLDFVGEVLANTLRAN
                     QRRISIDEIQTRVSEHYRIRKAEMTSARRAREVARPRQVAMYLSKQLTPRSLPEIGRR
                     FGGRDHTTVIHAVRQIEKLRALDSELDSDIRLLTRQLEG"
BASE COUNT          549 a         1150 c         1198 g          556 t
ORIGIN      
        1 ggcctatgtc ctcatcaaga tcctgtcccc cggtttctat gcgcggcgcg acatcaagac
       61 gccggtgttc gttgccgtgg cgaccctgct ggggagcgtc gtgctcaact tcatcctggt
      121 gccccggctc gggatcgtcg cgcttccact ttcgaccgcg atcggtgcct gggccaacgc
      181 gttcctgctc gccgccctgc tccatgcgcg cgggcacttc cgcctgccgg gctggctcgc
      241 ctcgcggctg gtacggcagc ttctcgccgg cgcggccatg ggggtgactt tgtggctgat
      301 cgagcgggcg ctggccgatc gcttcgccgg ctcggcgggc gaccgggtcc tggcgatggg
      361 agcgctcgtc gcggcggggg ggatcgtcta tttcgcagtc gcctggacga tcggagcgat
      421 gaaccgcgag gatatcttga tcctgctcag gcgcaagaag gtcgactgac ctgaaggcac
      481 cgatttgcgg cttgagcgca cctgtccgcg ccttcagccg ccgttcagcc gtcttttgta
      541 gaaaggcgcg gtttcctata tagttacagc tacggctccg ttcccaagag gatgcgtccc
      601 atgtctctcg ccaagaaatt gctcaccctc gccgcgccgg cggccctgct cgcgctgggc
      661 ggctgcgcga ccggtcttcc cacccaggtt tcccgctacc aggcgatgcc ggccccggcc
      721 ggacagagct tcgccatcca ggccgccgat cccgaggatc gcggcggcct cgaattctcc
      781 cgttatgccg agctcgtccg ccggcacatg atcgcccaag gctatagcga ggcggcctcg
      841 ccgcagcagg cgtcgctgat cgtctcgctc gactacgggg tcgacgacgg ccgccagcag
      901 gtcgtcacct atccgtcctc gcgcttcggc agccgcttcg gctatggcgg cttcggctat
      961 cggccctact ggtcgcgctt cggcggctac ggccggcttc gctcgccctt ctattacggc
     1021 tgggacgatc ccttctggta tcgcggcttc ggcgaggaga tcgacgtcta caccgtctat
     1081 tcgagcttcc tcgacatgga catccgccgc cccgacggcc aggccgtgtt cgaaggcacc
     1141 gcccgggcgc gctcgcgcac ggacgagctc agcgtgctcg tgcccaacct catcgaggcg
     1201 atgttcaccg gcttccccgg caattcgggc gagacggtga agatcacggt ggcgccggag
     1261 cgcaggcgct aagcggccgt cgcgtcactc cgtcattgcg cggagcgaag cgacgaagca
     1321 aaccagcggg agtccgaggc agcactggat tgtttcgctt cgctcgcaat gacggggagg
     1381 gccggcgacg gccctccctt ttctttgggc gccattcaac gggcgttcat cggccgagcc
     1441 ggatttcaag ccccgacagg ggaatgagaa aagtctcggg gagggtgaat gctgaaatat
     1501 aagggagcgg ccgcaatggc cgctatcgcg cttgcgtccg cggcgccggc gcagaacaag
     1561 gccgccgacc gcgcccaggt cgaggacatc gtcgtcaccg gccggaagag cggggtgccg
     1621 atgtggatcg tgcgcggcga cacgacgacg ctcgtcctcg tcggcgggat caacggggtg
     1681 tccaagacca cgcattggga tccggccgcg ctcaccgagg cgctgcgcaa ggccgaccgg
     1741 gtgatgttcc cgcaatccta cgctctgacc gcctcgccct tccgggcgat cggctggatc
     1801 gccaaatgga acaagatggg cagcctgccc aagaaccaga gtctcggggc catcgccgga
     1861 ccgcaggcga tgcgccggct ggcggcgctt cgggcgcgga acatggtccg ggcggattac
     1921 gaccggcgcc atccgctcca cctgtcgcag gaactgcgga gccgggccaa aggcgacatc
     1981 gactatagcc gcgacgtcgc cgaatatgcc cgcaacgctg cgaagaagca caagctcgcc
     2041 gaggtcgtcc cgatccagcg ctccaaggcc aagccggtgg tcaaggattt gttcgcctcg
     2101 accccgcagg agcatgtgcc gtgcctcgag gccgcactga cgatggccga ggccgggccg
     2161 gcggcggttc aggctcggtc cgacgcctgg gcggcgcggc gggtccgaga cgtgctcagc
     2221 tcgccggcgg acccggtcta cgagcgttgc tggcccagcg gagtcttcgc cgaccgcgaa
     2281 gtcgagctgc tcccgcagat gaagcaattg ctggccgagc cccaggtgac ggtggcggtg
     2341 ctcagcctgc gcaccctcgc cgagcagggc gggcttctgg acggactggc ggcggccggg
     2401 ttcgacatac aggggcccgc ctggaagtga cggcatagcc gtcattgcga gcgtagcgaa
     2461 gcaatccaga actgcggctt gccacaggct cagcgctgga ttgcttcgtc gctgcgctcc
     2521 tcgcgctgac gccgacggtc agcgcctgac caggtaagct ccggcaagga cgaggaggat
     2581 gccggcgatc cgggtcgggc cgatctcgac ccgggcgagg ccgaaggctc cgatccggtc
     2641 gaggaagaga gccatggcga gttggccggc gatcgccacc atcaggaaga aggtcacccc
     2701 catgcgcggt gccgcgaagg cggcgacggc gacgaagaag gcgccataga agccgccgac
     2761 ccaggcgtac cagggaagcg cgcccgtcac gccggcggcg ggcctcgcgc caagggccgc
     2821 tgccacggcg agcagggcga tggtgccgac gacgaaggaa acgaaagcgg cgttcacggc
     2881 cgagccgacc ccgcgggcga ggagggcatt ggtcggtgcc tgcagggcga tgaggcttcc
     2941 gcccacgaaa gcggccccca gggagaaaag aagggcggga ttcaagcgac ccggcccccc
     3001 ttgaccctag tccgaccgaa aacggctcaa ccctcgagct ggcgggtcag caggcggatg
     3061 tccgagtcga gctccgaatc cagggcccgc aatttctcga tctggcggac ggcgtggatc
     3121 accgtcgtgt ggtcgcgccc tccgaagcgg cggccgatct cgggaagcga gcgcggggtg
     3181 agctgcttgg agaggtacat ggcgacctgg cgcggccggg cgacctcgcg ggcgcggcga
     3241 gcgctggtca tctccgcctt gcggatccgg taatgctccg agacccgggt ctggatctcg
     3301 tcgatcgaga tccggcgctg gttggcgcgc agcgtgttgg cgagcacttc gcccacgaaa
     3361 tcgaggtcga tgacccggtt ctgcatggtc gcgtaggcgg cgatcctgtt gagggcgcct
     3421 tccagctcgc ggacgttgga attgatccgc ttg
//
LOCUS       DASWNU010000022         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_26438, whole genome shotgun sequence.
ACCESSION   DASWNU010000022 DASWNU010000000
VERSION     DASWNU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26438"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VGB57_00475"
     CDS             <1..955
                     /locus_tag="VGB57_00475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0129849.1"
                     /translation="MRQILLAALAASCLAGCSTDAGKAPEPAANSRYSFWRPAPVVDA
                     PDPAKAVQEAPAEAWRRIDPSNLLVIELKEGGRVSIELAPDFAPVHIANVRAFAKAGW
                     WNNASIYRVQDNYVSQWGNGDSKTELPAGVVRQPPHEYHRPLAGLQVRPLGFPDSYAP
                     MVGHAFGWPVGYDPEEGRAWLTHCYGYVGVGRDLAPDTGTGGELYAVIGHAPRHLDLN
                     IAVIGRVVEGMALLSARPRGTEALGFYKDPAQHIAIARARLAADIPAADRPAYEAMRS
                     DSLAFSQYVTGRANRGGEFFQRPAGGVDICNAQVPVRKVTP"
     gene            complement(1123..1389)
                     /locus_tag="VGB57_00480"
     CDS             complement(1123..1389)
                     /locus_tag="VGB57_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129850.1"
                     /translation="MATSILEARGGCIIPQADQRGYHIVYREHETNYCPGCGRTHWYI
                     GRSMAECAFCTTALPLENAPMIAGGAIVRTIRRPVYGNEQAYAA"
     gene            complement(1627..1782)
                     /locus_tag="VGB57_00485"
     CDS             complement(1627..1782)
                     /locus_tag="VGB57_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129851.1"
                     /translation="MEGLELGTVVLVSSLVLGALALPVTAVAIHQRRRRSFEKRAGVR
                     RKEKIQL"
BASE COUNT          344 a          702 c          655 g          319 t
ORIGIN      
        1 gatgcgccag atcctgctcg ccgccctcgc cgcttcctgc ctcgccggct gctcgaccga
       61 tgccggcaag gcgcccgagc ccgccgccaa ttcccgttat tccttctggc gcccggcgcc
      121 ggtggtcgac gcgcccgatc cggccaaggc ggtccaggag gctcccgccg aggcgtggcg
      181 gcgcatcgac ccgtccaacc tcctcgtcat cgagctgaag gagggcggcc gggtttcgat
      241 cgagctcgcg cccgatttcg cgcccgtcca catcgccaac gtccgcgcct tcgccaaagc
      301 cggatggtgg aacaacgcct cgatctaccg ggtccaggat aattacgtct cccaatgggg
      361 caatggcgac tcgaagaccg agcttcccgc cggggtagtc cggcagccgc cgcacgaata
      421 tcaccggccg ctcgccgggc tgcaggtccg ccccctcggc ttccccgaca gctatgcgcc
      481 gatggtcggc cacgcatttg gctggccggt cggctacgat cccgaggagg gccgggcctg
      541 gctgacccat tgctacggct acgtcggcgt cgggcgcgac ctggcgccgg acaccggcac
      601 cgggggagag ctctacgcgg tgatcgggca tgcgccccgc catctcgacc tcaacatcgc
      661 cgtcatcggc cgggtcgtgg aaggcatggc cctgctctcg gcccgcccgc gcggcaccga
      721 ggctctgggc ttctacaagg atccggcgca gcacatcgcc atcgcgcgcg cgcgcctcgc
      781 cgccgacatc ccggccgccg atcgccccgc ttacgaggcg atgcgctcgg acagcctcgc
      841 cttcagccaa tatgtgacgg gccgagccaa ccgcggcggc gaattcttcc agcgccccgc
      901 ggggggagtg gacatctgca acgcccaggt gccggtgcgg aaggtgacgc cgtaaaggcg
      961 tcattgcgag gagcgtaagc gacgcggcaa tccagtgggc ctcggaggcc gtactgggtt
     1021 gcttcgcttc gctcgcaatg acggggttac gcgccggccc ctcgcgtagg gatgataagg
     1081 ggcgggcgcg acggcggccg cccgagggcg ccgcccgcga tttcaggccg cgtaggcttg
     1141 ctcgttgccg tacaccgggc ggcggatggt gcggacgatg gcgccaccgg cgatcatcgg
     1201 agcgttctcg agcggaagcg cggtcgtgca gaaagcacat tccgccatcg accggccgat
     1261 ataccagtgg gtccgcccgc aaccggggca ataattggtc tcatgctcgc ggtagacgat
     1321 atgatagccg cgctgatccg cctgggggat gatgcagccg ccgcgcgctt caaggatact
     1381 cgtggccata cttggctccc ttctcactca cttgcggccc taacgaccga ggagtctgaa
     1441 gagtttcatg gcgttaagat gaacggagcc gccgagcggg tgtccggcgc ctcggcgaca
     1501 gggccgaaaa gggcggaaaa ctgcggcgac gaggcgttcg gaaccgtcgg cgaatcgagg
     1561 ctcaaaatat tttcgcgcgg cccccgttaa cctttttcgg gccggattcg gcttccgatc
     1621 cgggcctcaa agctggattt tttccttgcg ccggacaccc gcccgcttct cgaaggagcg
     1681 gcgcctgcgc tgatggatcg cgaccgccgt caccggcagc gcgagagcgc cgagcacgag
     1741 cgacgacacc aggaccacgg ttcccagctc cagaccttcc atcgaaactt tcctccaacc
     1801 acccgattag cttcatgcgc cgccgccgtc accctagtgc tgccgccgcc gggccgaaac
     1861 ggggctaagc aaggacgaaa gaggcggcgg cctgagcgat atcgacgaca ggaacccgtt
     1921 caaaaaaatt agaaccgttt cgagaccgat ccaatcgact ccgcggagcc gccgggccta
     1981 tcatgaggct tccaacaaag ggaggacttc catggcgact
//
LOCUS       DASWNU010000023         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_337942, whole genome shotgun sequence.
ACCESSION   DASWNU010000023 DASWNU010000000
VERSION     DASWNU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337942"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..452)
                     /gene="arfB"
                     /locus_tag="VGB57_00490"
     CDS             complement(36..452)
                     /gene="arfB"
                     /locus_tag="VGB57_00490"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY0129852.1"
                     /translation="MPPAAEPFVIPEEALEEKFLASTGPGGQNVNKVATAVQLRCDVF
                     KLGLEPPVYARLKVLAGSRMTGAGEIVITARSHRTQEANRAEARERLADLVARAHVRP
                     LKRKPTKPSRSAKAKRVDEKKGRGAVKQGRGKVRVD"
     gene            complement(436..1188)
                     /locus_tag="VGB57_00495"
     CDS             complement(436..1188)
                     /locus_tag="VGB57_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEY0129853.1"
                     /translation="MAVWDKYVLFIDGEAIVIDKPAGLAVHPGSRTPESLEDYLKHFR
                     FGFKRAPLPVHRLDRDTSGCLLLARNPKAHKRFQRAFEAKQVTKTYLAVVDGVPEGEE
                     GAIDMPLAKISSEAEGWRMVNDPDGRPSLTRWRRIAVRDGRAVILFSPETGRTHQIRV
                     HAADGLGIPISGDPVYGGSNGPMLLHALSIRVDRDGKPPVEATAPLPPTFVNAGLGDV
                     LAGEPHLQAPAALPPIFHEAGPSEDPGDAPGG"
     gene            complement(1263..2438)
                     /locus_tag="VGB57_00500"
     CDS             complement(1263..2438)
                     /locus_tag="VGB57_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0129854.1"
                     /translation="MTNDAAPVTVISSIGDPRDRRTWSTTPSEILAAMERSGIAVRTH
                     AIATPRPLGYVYLAGNMLSGYGRLQPNRVGLWSSHLRRSASRLIAGAPSGTFLHFGSD
                     HLPLSGTRPGQRHFLVTDYSIHLLMTQGVLGEKAPRRYRDAVLSAEKAIAAQMEAIFT
                     VSRYVADDWRTTYALGAEKVVPIGTGLGNPLTLEGAAKDYRGGHLLFVAKHSFDIKGG
                     PLLVQGFLEALKSRPDLRLVVIADAGDPTLAPWLPTMQTHPSIDFRQSGTPDFVDLMR
                     RAILFVGPAEREPWGLVYLECLMCETPILGLDRNAMRELSDEGRVGFIVEEASAAAVG
                     RAIVDAVSDPERLARMGRSGREFVEAGFTWDQVAARMLRRMGLDCGPADAAPSRPAD"
     gene            2713..3768
                     /locus_tag="VGB57_00505"
     CDS             2713..3768
                     /locus_tag="VGB57_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HEY0129855.1"
                     /translation="MASEKIDPSADRNRGAVVLTSIAPPNDVMRRIASDCRERGIRFY
                     VVGDTKSPADFALDGCEFFSVDAQVASGSRHAELCPTRHYARKNFGYLMAMRQPIDFI
                     VETDDDNMPSDDFYRPLPIDLAVPSVSGCGWLNVYRYFTDQLIWPRGLPLDTINAPLP
                     EPVDGEMIRAPIQQGRADENPDVDAIFRLVLPLPFTFEKRLRLALGDGAWCPFNSQNT
                     VFYREAFPLLYLPAYCSFRMTDIWRSFVAQRIAWANGWSVLFREATVVQDRNDHSLMR
                     DFEDEISGYLGNEKIRQALQALDLPSGVEAIPDAMRMCYRALTDQGFVGAEELPLLDA
                     WLEDLERFGRGNTRTPG"
     gene            3816..4886
                     /gene="gmd"
                     /locus_tag="VGB57_00510"
     CDS             3816..4886
                     /gene="gmd"
                     /locus_tag="VGB57_00510"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0129856.1"
                     /translation="MTERKVALITGVTGQDGAYLSELLLSKGYEVHGVKRRSSSFNTG
                     RIEGIYQDPHVEGARFHLHYGDLTDSTNLIRLVQQTQPTEIYNLAAQSHVGVSFETPE
                     YTANADGIGTLRLLEAIRILGMEKSCRFYQASTSELYGLVQEVPQKETTPFYPRSPYG
                     AAKMYAYWIVVNYREAYGMHASNGILFNHESPLRGETFVTRKITRAAAAIKLGRQERL
                     WLGNLDAKRDWGHAREYVRGMWLMMQQEEPDDYVLATGVTTQVRTFVEWAFEDVGIAL
                     RWEGEGVDEKAYCAESGRCLVEVDPRYFRPTEVELLIGDATKARERLGWSHETPVREL
                     VREMVVEDLKVMRDAPIGKDAR"
     gene            4889..5845
                     /locus_tag="VGB57_00515"
     CDS             4889..5845
                     /locus_tag="VGB57_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEY0129857.1"
                     /translation="MASAFTLAGKRVFVAGHRGMVGSALVRRLAREDCEIITASRDEL
                     DLKDQRAVRDWMAEKRPQAVFLAAAKVGGILANDSYPADFLYDNLMIEANVIEAAHRV
                     EVEKLLFLGSSCIYPKFAPQPITEDALLTGPLEPTNEWYAIAKIAGIKLAQAYRRQHG
                     RDFISAMPTNLYGPGDNFALNSSHVLPALIRKAHEARSSGAPLVVWGTGSPRREFLYV
                     DDCADACVLLMNSYADAQHVNVGSGEDISILDLTRLVCKVVGYTGPIELDPSKPDGTP
                     RKLMDAGKLNGMGWAPSVSLEDGIARAYAWFLDNHEAQAVPA"
BASE COUNT          967 a         1940 c         1967 g          989 t
ORIGIN      
        1 gccggcgatc gccggggaag gctgtggcga gccgttcagt ccacccgaac cttgccccgc
       61 ccctgcttga ccgctccgcg gcccttcttc tcgtccaccc gctttgcctt ggcggaccgg
      121 ctcggcttgg tcggtttgcg cttcaagggg cggacgtggg cgcgggcgac gaggtccgcg
      181 agccgctcgc gcgcctcggc gcgattggcc tcctgggtgc ggtggctgcg ggcggtgatg
      241 acgatttcgc cagcccccgt catccggctg ccggcgagca ccttcagccg cgcataaacg
      301 ggcggctcga gccccagctt gaacacgtcg catctgagct gcaccgcagt cgcgaccttg
      361 ttgacgttct ggccgcccgg gccggtcgag gcgaggaact tctcctccag cgcctcctcg
      421 gggatgacga agggctcagc cgccgggggc atcgcccgga tcctcgctcg gccccgcctc
      481 gtggaagatc ggtgggaggg cggccggcgc ctgcaggtgc ggctcgccgg ccaagacgtc
      541 gccgaggccg gcgttgacga aggtcggcgg caagggcgcg gtcgcctcga ccgggggctt
      601 gccgtcgcgg tcgacccgga tcgacaaagc gtggaggagc atcgggccgt tcgagccgcc
      661 gtagaccggg tcgcccgaga tcgggatccc gaggccgtcg gccgcgtgga cccttatctg
      721 gtgggtccgg ccggtctcgg gcgagaagag gatgacggcc cggccgtccc ggacggcgat
      781 cctgcgccag cgggtcagcg agggcctgcc gtcggggtcg ttcaccatcc gccagccttc
      841 ggcctcgctc gaaatcttgg cgagcggcat gtcgatcgcg ccttcctccc cttcgggcac
      901 tccgtcgacg acggcgagat aggtcttggt cacctgcttc gcctcgaagg cgcgctggaa
      961 gcgcttgtgg gcttttggat tgcgggcgag gaggaggcag cccgaagtgt cgcggtcgag
     1021 ccggtggacg ggcaggggcg cgcgtttgaa gccgaagcgg aagtgcttca gatagtcttc
     1081 gaggctctcg ggcgtgcgcg agccggggtg gacggcgaga cccgccggct tgtcgatgac
     1141 gattgcctcc ccgtcgatga acaggacata tttatcccac acggccattg tccttcatcg
     1201 ggcggccctg ccacgcctgc cgcggcggcg caaaacctga atcgcggccc gtggctttcg
     1261 ggtcaatccg cgggacgtga aggggctgca tcggccgggc cgcagtccag ccccatccgg
     1321 cggagcatcc tggccgccac ctggtcccag gtgaaaccgg cctcgacgaa ctcgcggccc
     1381 gagcggccca tccgggcgag cctctccggg tcggatacgg cgtcgacgat cgcccggccc
     1441 acggcggcgg cgctcgcctc ctcgacgatg aatcccaccc ggccctcgtc gctcagctcg
     1501 cgcatggcat tgcggtccag ccccaggatc ggggtctcgc acatcaggca ctccagatag
     1561 acgaggcccc agggctcccg ctcggcgggc ccgacgaaga ggatcgcgcg ccgcatcagg
     1621 tcgacgaagt ccggggtgcc cgattgacgg aaatcgatcg acggatgggt ctgcatcgtc
     1681 ggcagccagg gcgctagggt agggtccccg gcgtcggcga tgacgacgag gcggaggtcg
     1741 gggcggcttt tcagggcttc gaggaagccc tggacgagca gcggcccgcc tttgatgtcg
     1801 aagctgtgct tcgccacgaa gagcagatgg ccgccccggt aatccttggc cgcgccttcg
     1861 agcgtgaggg gattgcccag gccggtgccg atcggcacca ccttttcggc gccgagcgcg
     1921 taggtggtgc gccagtcgtc cgcgacgtat cgggagacgg tgaatatcgc ttccatctgg
     1981 gccgcgatgg ccttttcggc ggagagcacc gcatcccggt accggcgagg cgccttctcg
     2041 ccgaggacgc cctgggtcat cagcaggtgg atactgtaat cggtgacgag gaaatgccgc
     2101 tgaccgggac gggtccccga caagggcagg tggtccgagc cgaagtgaag gaaggtgccg
     2161 gatggcgcgc ctgcgatgag ccgcgaggcc gaccggcgca ggtgcgagct ccacagcccg
     2221 acccggttcg gctgaaggcg gccgtaaccg ctcagcatat tgccggcgag gtagacgtag
     2281 cccagcggac gaggggtcgc gatagcgtgg gtgcgaacgg cgatgcccga gcgctccatc
     2341 gcggcgagga tctccgacgg ggtggtcgac caggtgcgcc tgtcgcgtgg atcgccgatc
     2401 gaggagatca ccgtcaccgg cgcggcgtcg ttcgtcatta cggctttccc ttcaggcgat
     2461 cggcaccgct agcagcgccc ccggccgacc gacaaggggc cgcaagccct gcgctgttgc
     2521 aaagccttgt cgatcgctct acccggcccc cggctttccg gagttcatgc cgaggcgtgg
     2581 gccgcgatcc tgtgctgcag gccaacgcag aacgcgcgtc gcaaggcggc cagaatccac
     2641 cctttcagcg gttccggcgg accgggcggc acgggccgga tgccgggcgc tccgcttttc
     2701 atgaggtttt ccatggcatc cgagaagatt gatccttccg ccgatcgaaa ccggggcgcg
     2761 gtagtcctga cctccatcgc tccgcccaac gacgtcatgc ggcgcatcgc ctcggattgc
     2821 cgggagcgcg gcattcgctt ctatgtcgtc ggcgacacca agagtccggc cgacttcgcg
     2881 ctcgacggct gcgaattctt cagcgtcgac gcccaggtcg ccagcggctc gcgccatgcc
     2941 gagctttgcc cgacccgcca ttatgccagg aagaatttcg gctatctgat ggcgatgcgg
     3001 cagccgatcg atttcatcgt cgagacggac gacgacaata tgccttccga cgacttctac
     3061 cgcccgctgc cgatcgacct tgccgtgcca tcggtgagcg gctgcggctg gctgaacgtc
     3121 tatcgctact tcacggacca gctcatctgg cctcgcggcc tgccgctcga cacgatcaat
     3181 gccccgcttc cggagccggt ggacggagag atgatccggg ccccgatcca gcagggccgc
     3241 gccgacgaga atcccgacgt cgatgcgatc ttccggctcg tcctgccttt gccgttcacc
     3301 ttcgagaaga ggctgaggct ggcgctcggc gacggcgcct ggtgtccgtt caacagccag
     3361 aacacggtct tctaccggga agccttcccg ctcctctacc tgccggccta ttgcagcttc
     3421 cggatgacgg acatctggcg cagcttcgtc gcccagcgca tcgcctgggc gaacggctgg
     3481 tcggtcctgt tccgcgaggc caccgtcgtc caggaccgca acgatcacag cctgatgcgg
     3541 gatttcgagg acgagatcag cggctatctc ggcaacgaga aaatccgcca ggcgctgcag
     3601 gcgctcgacc tgccctccgg cgtcgaagcg attcccgacg ccatgcggat gtgctaccgc
     3661 gcgctcacgg accagggctt cgtcggcgcc gaggagctgc ctctgctcga cgcctggctc
     3721 gaggatctcg aacggttcgg ccgcgggaac acccggacgc ccggctgagc cggcgctggc
     3781 attaccccaa cttgaattga gaaatgagga cgctggtgac cgagcgcaaa gtagcattga
     3841 taacgggagt taccggccag gatggagcct atctgtcgga attgctgctc tcgaaaggct
     3901 atgaggttca cggcgtcaag cggcgctcgt cttcgttcaa cacggggcgc atcgagggca
     3961 tctatcagga tccgcacgtc gagggcgcgc gcttccattt gcattatggc gatctcaccg
     4021 attcgaccaa cctgatccgg ctcgtccagc agacccagcc gaccgaaatc tacaacctcg
     4081 ccgcccagag ccatgtcggc gtgagcttcg agacgcccga atataccgcc aatgccgacg
     4141 gcatcggaac gctccgcctt ctcgaggcga tccggatcct gggaatggaa aagagctgcc
     4201 gtttctatca agcctcgacg tccgagctct acggcctggt tcaggaggtg ccgcagaagg
     4261 agacgacgcc cttctacccg cgctcgccct acggcgcggc caagatgtac gcttattgga
     4321 tcgtggtgaa ctaccgcgag gcctatggaa tgcacgcgtc gaacggcatc ctcttcaatc
     4381 acgagagccc gctgcgcggc gagaccttcg tcacccgcaa gatcacccgg gccgcggcgg
     4441 cgatcaagct tggccggcag gagaggctct ggctgggcaa cctcgacgcg aagcgggatt
     4501 ggggccatgc ccgcgaatat gtccggggca tgtggctgat gatgcagcag gaggagccgg
     4561 acgattatgt cctcgccacc ggagtcacga cgcaggtgcg caccttcgtc gagtgggcgt
     4621 tcgaggatgt cggcatcgcc ttgcgctggg agggggaagg cgtcgacgaa aaggcctatt
     4681 gcgccgagag cgggcggtgc ctcgtcgagg tcgatccccg ctatttcagg ccgacggaag
     4741 tcgagcttct gatcggcgac gccaccaagg cgcgggagcg gctgggctgg tcgcatgaaa
     4801 ccccggtccg cgagctcgtt cgcgaaatgg tggtcgagga tctcaaggtc atgcgggacg
     4861 cccccatcgg aaaggacgcc cgatagccat ggcctcggct ttcacgctgg ccggcaagcg
     4921 cgtcttcgtc gcgggccacc ggggcatggt gggatccgct ttggtccggc ggctggcccg
     4981 cgaggattgc gagataatca ccgcgtcccg cgacgagctc gacctcaagg atcaacgcgc
     5041 ggtccgcgac tggatggcgg aaaagagacc gcaggccgtt ttcctggccg cggccaaggt
     5101 cggcggcatc ctggcgaacg attcctaccc cgccgacttt ctttacgaca acctgatgat
     5161 cgaggcgaac gtcatcgagg cggcgcaccg cgtcgaagtg gagaaattgc tcttcctggg
     5221 atcgagctgc atctatccca agttcgcgcc gcagccgata accgaggacg ccttgctcac
     5281 gggtccgctc gagccgacca acgaatggta tgcgatcgcg aagatcgccg gcatcaagct
     5341 ggcgcaggcc tatcgccgcc agcacggccg cgacttcatc tcggccatgc cgaccaacct
     5401 ctatggcccg ggcgacaatt tcgctctgaa ttccagccat gtcctgccgg ccctgatccg
     5461 caaggcgcat gaagccagat cgtccggagc tccgctcgtc gtctggggca cggggtcgcc
     5521 gagacgcgaa tttctctacg tggacgactg cgccgatgcg tgcgtcctgc tgatgaactc
     5581 ctatgccgat gcgcagcacg tcaatgtcgg ctccggcgaa gacatctcga tcctcgatct
     5641 gacccgtctc gtctgcaagg tggtgggcta taccggcccc atcgagctgg acccctcgaa
     5701 gcccgacggc acgccccgca agctgatgga cgccggcaag ctcaacggta tgggatgggc
     5761 gcccagcgtg tccctggaag acggcatcgc ccgcgcttac gcctggttcc tggacaatca
     5821 cgaagcacag gcggtgccgg cctagagtgt atggttcctt aaa
//
LOCUS       DASWNU010000024         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_208330, whole genome shotgun sequence.
ACCESSION   DASWNU010000024 DASWNU010000000
VERSION     DASWNU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208330"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VGB57_00520"
     CDS             complement(<1..946)
                     /locus_tag="VGB57_00520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY0129858.1"
                     /translation="MKALLSTAAGGPETLELADLPDPEPGPGQLLVRVRACAINYPDV
                     LIIEDKYQFKPPRPFAPGGEVAGTVEAVGEGVSGWAEGDRLIAMIGHGGLAEKVVVSA
                     AQAVPLPPERSFEDGSALILTYATSIHALLDRGRMKPGETLLVLGAAGGVGLAAVELG
                     KAYGARVIAAVSSEAKAEVARQAGADLAIVYPSGPFDKDGSKALAQMFKEAVGPGGAD
                     LVYDPVGGDYAEPALRSIAWEGRYLVVGFPAGIPKLPLNLTLLKSCDVRGVFWGAFAA
                     RDPKANAAHIETLFGLWREGKIAPRVSRTWPLERGGEAI"
     gene            complement(943..1782)
                     /locus_tag="VGB57_00525"
     CDS             complement(943..1782)
                     /locus_tag="VGB57_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY0129859.1"
                     /translation="MIKKLTLLAAAWLGLGAMQAPATPQAPIELWRLDCGEFVIKQYG
                     AFFSDTFQYPPGPKNIVGSCYLIRHGSRYMLWDTGLTDALIGAGFENAGQKISVKRSL
                     VDQLAQIGVKPAQIELMGISHWHFDHTGQAPRFPQATLLMGKGDLELLRGTPSPDEDS
                     TKALAHWLSGGGKADPLTADKDVFGDGRVVMLKLPGHTPGHYALLVRLASGPVLLSGD
                     QYHFTEQVKNRGVPPFNHDRSDTLASSDRFDRIAANHKAKEINQHEPADIAKLPVFPK
                     SAK"
     gene            complement(1779..>2088)
                     /locus_tag="VGB57_00530"
     CDS             complement(1779..>2088)
                     /locus_tag="VGB57_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0129860.1"
                     /translation="GFGTYIVSAPPPFSPEDCGELALDAAAVVERYFPEAAALYARAS
                     WTLPAQIDRVYVPAAAEAELGFRCRTDFAAILAALAKDGPLPFVHDPHYVSPKQGTPT
                     "
BASE COUNT          325 a          700 c          736 g          327 t
ORIGIN      
        1 cgatcgcctc gccgccccgc tcaaggggcc atgtccggct gaccctgggc gcgatcttgc
       61 cctcgcgcca caggccgaac agggtctcga tatgcgcggc attcgccttg ggatcgcgcg
      121 ccgcgaaggc gccccagaag actccgcgga cgtcgcagct cttcaggagg gtgaggttga
      181 gcggaagctt cgggattccg gccggaaagc cgacgacgag gtagcggcct tcccaggcga
      241 tcgagcgcaa ggccggctcg gcatagtcgc cgccaaccgg atcgtagacc aggtccgcgc
      301 cgcccggtcc gaccgcctcc ttgaacatct gggcgagcgc cttcgacccg tctttgtcga
      361 aaggcccgga cgggtagacg atcgccaggt ccgctccggc ctgccgggcg acctccgctt
      421 tcgcctcgga ggagacggcg gcgatcacgc gcgcgccata ggccttgccg agctcgaccg
      481 cggcgagccc gaccccgccg gcggcgccga ggacgagcag ggtctcgccc ggcttcatcc
      541 ggccccggtc gagcaaggca tggatcgagg tcgcgtaggt caggatcagg gccgagccgt
      601 cctcgaagct ccgctcgggc ggcagcggca ccgcctgggc ggcggagacg accaccttct
      661 ccgcgaggcc gccatggccg atcatcgcga tcagccggtc gccctccgcc cagccggaga
      721 cgccctcccc caccgcctcg accgttccgg cgacctcgcc gcccggagcg aacgggcgcg
      781 gcggcttgaa ctgatatttg tcctctatga tcaggacgtc gggataattg atcgcacagg
      841 ccctcacccg gacgagaagc tggcccgggc cgggttcggg atcgggaaga tcggcgagct
      901 cgagcgtctc cggcccgccc gccgccgtcg aaagcagcgc cttcacttcg ccgatttcgg
      961 gaagacggga agcttggcga tgtcggccgg ctcgtgctgg ttgatctcct tcgccttgtg
     1021 gttggcggcg atccggtcga accggtccga cgatgcgagc gtgtcggagc gatcgtggtt
     1081 gaacggcggg acgccgcgat tcttcacctg ctcggtgaag tgatactggt ccccggagag
     1141 gagcaccggt cccgaagcca ggcggacgag cagggcgtaa tggccgggcg tgtggcccgg
     1201 cagcttcagc atgacgaccc ggccgtcgcc gaagacgtcc ttgtcggcgg tcagcggatc
     1261 ggccttgccg ccgccgctga gccagtgcgc cagcgccttg gtggaatcct catcgggcga
     1321 cggggtgccg cgcagcagct ccaggtctcc cttgcccatg agcagggtcg cctgcggaaa
     1381 gcggggcgcc tgcccggtgt ggtcgaaatg ccagtggctg atccccatca gctcgatctg
     1441 cgcgggcttc acaccgattt gcgccagctg gtcgacgagg ctgcgcttga cgctgatctt
     1501 ctggcccgca ttttcgaacc ccgctccgat gagggcgtcg gtgaggccgg tgtcccacag
     1561 catgtagcga ctgccgtggc ggatcagata gcagcttccg acgatgttct tcgggcccgg
     1621 cggatattgg aaggtgtcgg agaagaaggc gccatattgc ttgatcacga actcgccgca
     1681 gtcgagccgc cacagctcga tcggcgcctg cggagtcgcc ggcgcctgca tcgcgccgag
     1741 gccgagccag gcggcggcga ggagtgtcag tttcttgatc atgtcggtgt cccttgtttg
     1801 ggcgagacat aatggggatc gtggacgaag ggcaatggcc cgtccttcgc caaagcggcg
     1861 aggatcgcgg cgaagtcggt ccggcagcgg aagccgagct ccgcttcggc ggcggccggg
     1921 acatagaccc ggtcgatttg ggcgggcagg gtccagctgg cgcgggcgta gagcgcggcc
     1981 gcttccggga aatagcgctc gacgacggcc gccgcgtcca gcgccagctc gccgcaatcc
     2041 tccggcgaga aaggcggcgg cgccgagacg atataagtcc cgaagccc
//
LOCUS       DASWNU010000025         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_182363, whole genome shotgun sequence.
ACCESSION   DASWNU010000025 DASWNU010000000
VERSION     DASWNU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182363"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1162)
                     /locus_tag="VGB57_00535"
     CDS             complement(317..1162)
                     /locus_tag="VGB57_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0129861.1"
                     /translation="MSRWSFLPVAAASALLAAPAAAAPFKPTRISVVVRGSGPDVILI
                     PGLSSSRGVWNGTVAAVPGYRYHLVQVAGFAGTPAAGNNAGRVAGPVADEIAGYIAAS
                     RLGRPAIVGHSMGGSIAMMIGARHPGVAGRVMVVDMLPSPARPFGIPSAAMKPLAKLV
                     GGEMAGAGRLRSDLKSLVGRFGNSDWLESRNDAGVVGRSVSELLSTDLAAELPRIRAP
                     LTVVYACSARLSYACAPVGRAYASAYSRRPGTRLVRIDRSGHTPMWDQPAAFQAALRG
                     FLGGR"
     gene            1340..>2287
                     /locus_tag="VGB57_00540"
     CDS             1340..>2287
                     /locus_tag="VGB57_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY0129862.1"
                     /translation="MPHLYLVDGSSYIFRAYHRLPPLTNRHGLNVGAVYGYTTMLWKL
                     AESLHKEEGPTHLAVIFDAGSHTFRNDMFEGYKAQRPPPPPDLVPQFPLIRDATRAFS
                     LPCIEEDGLEADDIIACYSKAALAAGWRVTIVSSDKDLMQLVEDGKLDLYDTMNDRRI
                     GRKYVIEKFGVPPEQLGEVLALMGDSVDNIPGVPGVGPKTAALLIQQYGDVETVLAHA
                     GEIKKPKLKQNLIEHAEAARMSRELVRLVCDSPLPEPLENLEMKGIPDGPLREFLEDQ
                     GFKALLARLNGTSQVKAPSDSATPVQREVRPEPKIDRSLY"
BASE COUNT          409 a          767 c          782 g          329 t
ORIGIN      
        1 gacgtcggga ccgctcccct gaacgaccac caagaagcgg gtcggagcga agggctttac
       61 ggcgggcgcg gagggcgagg gcgagggcgc ggcggctccg acgctggcga ggcccagggc
      121 ggcggcaatc aggatcggcc atttcatgat ccggcccgta agaggaccgg gttgaacgct
      181 gccttactcg ccccctgccg tcatgctgaa cttgtttcag cgtccagggt cgaaccgcct
      241 cgcgggtgga gaactggacc ctgaaacaag ttcaggatga cgatttgggc aaatggccgg
      301 acctttcgcc tgacgctcag cgcccgccca ggaagccgcg caatgccgcc tgaaacgcgg
      361 cgggctggtc ccacatcggg gtgtggccgc tgcggtcgat ccggaccaac ctggtgccgg
      421 gccgcctcga ataagcggag gcgtaggccc gacccaccgg agcgcaggcg tagctgagcc
      481 tcgccgagca ggcgtagacc acggtgagcg gggctcggat tcgcggcagc tcggcggcca
      541 ggtcggtcga gagcagctcc gagaccgagc gtccgacgac cccggcgtcg ttccggcttt
      601 cgagccagtc ggaattgccg aaccggccga ccagcgattt gaggtcgctc cggagccggc
      661 cggcccccgc catctcgccg ccgaccagct tcgccagcgg cttcatcgcc gccgaaggaa
      721 ttccgaaggg gcgagctggc gacggcagca tgtcgaccac catgacccgg ccggcgactc
      781 cgggatggcg cgcgccgatc atcatcgcga tcgagccgcc catcgaatgg ccgacgatgg
      841 cggggcggcc gaggcggctg gcggcgatat agccggcgat ctcgtcggcg accggtccgg
      901 cgacccgccc ggcattgttg ccggccgcgg gcgtgccggc gaagccggcg acctggacca
      961 ggtggtagcg atagcccggg acggcggcga cggtgccgtt ccacacgccg cgagacgagg
     1021 agaggccggg gatcaggatc acgtcgggcc cgctgccgcg cacgacgacg gagatccggg
     1081 tcggcttgaa cggcgcggcg gcggcgggcg cggcgagcag cgcagaggcg gcagcgacgg
     1141 gaaggaacga ccagcggctc atggggtgaa cctagcgcgg cgtggttaac gaatcccaga
     1201 ccgtagaatt cctacccgtc attccggcga aagccggaat ccatgaacac cggcagtgcc
     1261 ggcttgccgc gaaccgtgtt tcatggatcc cggccttcgc cgggatgacg aggcgtcccg
     1321 gctgcgctag agcctcccaa tgcctcatct ctatctggtc gacggttcca gctacatttt
     1381 ccgcgcctat caccggctgc cgccgctcac gaaccggcac ggcctcaacg tcggcgccgt
     1441 ctacggctat acgacgatgc tctggaagct cgccgagagc ctccacaagg aggaagggcc
     1501 gacccacctc gctgtcatct tcgacgccgg ctcgcatacc ttccgcaacg acatgttcga
     1561 aggctataag gcgcagcggc cgccgccgcc gccggacctc gtcccgcaat tcccgctgat
     1621 ccgcgacgcc acccgcgctt tttccctgcc ctgcatcgag gaggacgggc tcgaggctga
     1681 cgacatcatc gcctgctatt ccaaggccgc gctggcggcg ggatggcggg tcaccatcgt
     1741 ctcgtcggac aaggacctga tgcagctcgt cgaggacggc aagctcgacc tctacgacac
     1801 gatgaacgac cgccgcatcg gccgcaagta tgtgatcgag aagttcggag tgccgcccga
     1861 gcaattgggc gaggtgctgg cgctgatggg cgacagcgtc gacaacatac ccggcgtacc
     1921 cggcgtgggg ccgaagacgg ccgctttgct aatccagcaa tatggcgacg tcgaaaccgt
     1981 gctcgcccat gccggggaga tcaagaagcc caagctcaag cagaatttga tcgagcatgc
     2041 cgaggcggcg cggatgtcgc gcgagctcgt ccgcctggtc tgcgactcgc cattgcccga
     2101 accgctcgag aacctcgaga tgaagggcat tccggacggc ccgcttcggg aattcctgga
     2161 agaccagggc ttcaaggcgc tgctcgcccg cttgaacggc acctcgcagg tcaaggcgcc
     2221 gagcgatagc gcgacaccgg tgcaacgcga ggtccggcct gagccgaaga tcgaccgttc
     2281 gctctac
//
LOCUS       DASWNU010000026         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_182366, whole genome shotgun sequence.
ACCESSION   DASWNU010000026 DASWNU010000000
VERSION     DASWNU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182366"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1172)
                     /locus_tag="VGB57_00545"
     CDS             complement(66..1172)
                     /locus_tag="VGB57_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY0129863.1"
                     /translation="MSLHDPKAINIAIVGVGNCASSLVQGLAHYQNGGANDQIGLMHW
                     DLGGYRPKDIRIVAAWDIDRRKVGRDVAGAIFEKPNCTLVFCDHVPESGVTVEMGRTL
                     DGFSEHMAEWPDERTFLPADLPQPDKADVVRRLKETETHVLLNYLPVGSQEATEFYAE
                     CALEAGVAFVNNIPVFIASDEAWAERFRKAGVPIIGDDIKAQLGATIVHRVLTDLFRK
                     RGVRLERTYQLNTGGNTDFLNMSNRKRLASKKISKTEAVQSVAERRIDDDNIHVGPSD
                     YVAWQNDNKVCFLRMEGSMFGGVPMNLEMRLSVEDSPNSAGVAIDMIRCAKIAKDRGL
                     AGPVHPAAAYFCKHPPVQTTDDEAHEALERFIRG"
     gene            1540..2661
                     /locus_tag="VGB57_00550"
     CDS             1540..2661
                     /locus_tag="VGB57_00550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0129864.1"
                     /translation="MKPSLRILITTDSAGDLWQYSLDLARGLSRLGHETVLALTGPAA
                     TTEQIGTATAVPRLRFVDAGLGLDRLAGEEDSLVRAAEAIADLAAEADVDIIQLNTPA
                     LAALADFGRPVVAVLHSCVASWWEAVQGTALPADLAWRTDLIARGLRAAGAVVAPTAA
                     FGALVKRHYALDSAPRTVHYGRSPLAVPNGMPHDFVFTAGRLWDEGKNFGMLDAAAGR
                     IGVPVRAAGPLAGPNGAQVLFDNIHCLGTLGEEELGRWLSARPVFVSAALHEPFGPSV
                     LEAASAGCALILSDIPTFRELWDEVAIFVDPRDEDGFARAIGDLVGDDFERAVLGRAA
                     RERAARYTPDAMAAQMANLYRCLLPAVRRPVLAAARAAA"
BASE COUNT          399 a          939 c          908 g          471 t
ORIGIN      
        1 cgccagctac ccctccacca tgcttcgcac ggtccccctc cccatctggc gatggggagg
       61 caagactagc ccctaatgaa ccgctccagc gcctcgtgcg cctcgtcgtc ggtcgtctgc
      121 accgggggat gcttgcagaa ataggcggcc gccgggtgga ccggccccgc caggccgcgg
      181 tccttggcga tcttggcgca gcggatcatg tcgatcgcga ctccggccga attggggctg
      241 tcctcgaccg aaaggcgcat ctcgaggttc atcggaacgc ctccgaacat cgagccttcc
      301 atcctcagga agcagacctt gttgtcgttc tgccacgcga cgtaatcgga cggcccgaca
      361 tggatgttgt cgtcgtcgat ccgccgctcg gcgacggact ggaccgcctc ggtcttcgag
      421 atcttcttgg aggcgagccg cttgcggttc gacatgttga ggaagtcggt gttgccgccg
      481 gtattgagct ggtaggtgcg ctcgagccgg acgccgcgct tcctgaacag gtcggtgagc
      541 acccggtgga cgatggtcgc gccgagctgg gccttgatgt cgtcgccgat gatcggaacg
      601 cccgccttgc ggaagcgctc ggcccaggcc tcgtccgaag cgatgaacac cgggatgttg
      661 ttgacgaacg ccactccggc ttcgagcgcg cattcggcgt agaattcggt cgcctcctgg
      721 ctgcccaccg gcaggtagtt gaggaggacg tgggtctcgg tctccttcag ccgccggacg
      781 acatccgctt tatcgggctg aggcagatcg gccggaagga aggtgcgctc gtcgggccat
      841 tcggccatat gctcggaaaa gccgtcgagg gtgcggccca tctcgaccgt cactccgctt
      901 tcggggacat ggtcgcagaa caccaatgtg cagttgggct tctcgaagat ggcgccggcg
      961 acgtcgcggc ccaccttgcg gcggtcgatg tcccaggcgg cgacgatcct gatgtccttg
     1021 ggccggtagc cgccgagatc ccagtgcatc agcccgatct ggtcgttggc tccgccattc
     1081 tggtaatggg ccagcccctg cacgagcgag ctcgcgcaat taccgactcc gacgatggcg
     1141 atgttgatcg ccttggggtc atgaaggctc atcgggcgcg ctccagccgg gcgcggttaa
     1201 tataggttat cacgttcacc tcaaaggtcc tgcggcggcc tccaaacgcg tggagccacg
     1261 gaatcggcac atctacgcgg gctctgcccc gccggagcgc ggagtgcaag cttggcgttc
     1321 gccacgggga cgctaagcac atggcgcacc tgccctaaat cgcggtccgg gcgggaacaa
     1381 tgccttgtgc cggtcggttt aaccgggaag cgcagtccgg cgcccccgac ccatgttccc
     1441 ctccccagat ttcctcaaag atgcacagga agacgaaggc ttgccgaaac gccttttgat
     1501 tgagaccggc acggccgtct ccgacccgcg gcagcggcca tgaagccttc gctgcgcatt
     1561 ctcatcacca ccgattcggc aggcgacctg tggcaatata gcctcgatct cgcccgcggc
     1621 ctttcccgcc tgggccatga gacggtgctg gcgctgaccg gccccgccgc gacgaccgag
     1681 cagatcggga cggccacggc ggtcccgcgc cttcgcttcg tcgacgccgg cctcggcctg
     1741 gaccggctcg ccggcgagga agactcgctg gtccgggcgg cggaagcgat cgccgacctc
     1801 gccgccgagg cggacgtgga catcatccag ctcaacacgc ccgcgctcgc cgcgctcgcg
     1861 gacttcggca ggcccgtcgt ggccgtcctg catagctgcg tcgccagctg gtgggaggcc
     1921 gtgcaaggca cggcgctgcc cgccgatctg gcctggcgca ccgacctgat cgcccgcggg
     1981 ctgcgagcgg ccggcgccgt ggtcgctccc accgccgcct tcggcgccct ggtgaagcgc
     2041 cattacgccc tcgattccgc ccctcgtacc gtccattacg gccgctcccc gctggcggtg
     2101 ccgaacggca tgccgcacga tttcgtgttc accgccggcc ggctctggga cgagggcaag
     2161 aatttcggaa tgctcgacgc cgccgccgga aggatcggcg ttccggtgcg cgccgccgga
     2221 ccgctcgccg ggccgaacgg cgcccaggtc ctgttcgaca atatccactg cctcggcacg
     2281 ctcggcgaag aggaattggg ccgctggctc tcggcgcgtc cggtcttcgt ctccgccgcc
     2341 ctgcacgagc ctttcggtcc ctcggtcctc gaagcggctt cggcgggctg cgcgctcatc
     2401 ctctcggaca tcccgacctt ccgcgagctg tgggacgagg tcgcgatctt cgtcgacccg
     2461 cgcgacgaag acggcttcgc ccgggcgatc ggcgatctgg tcggggacga tttcgagcgg
     2521 gcggtgcttg gccgggcggc cagggagcgg gcggcgcgct acacgccaga cgcgatggcc
     2581 gcgcaaatgg caaatctgta tcgctgcctg ctccccgccg tgcgccggcc ggtgctggcg
     2641 gcggcgcggg cggcggcctg agcttctcaa tcctccccga gatttctcgg ggagggggac
     2701 cgcgcgaagc gcggtgg
//
LOCUS       DASWNU010000027         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_260149, whole genome shotgun sequence.
ACCESSION   DASWNU010000027 DASWNU010000000
VERSION     DASWNU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260149"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..380)
                     /locus_tag="VGB57_00555"
     CDS             complement(36..380)
                     /locus_tag="VGB57_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0129865.1"
                     /translation="MSEERPVYLRLRDNIAAMILEGRVGDGDPLPSVRSLAAEFGANP
                     LTVAKAYQTFQDEGLVLVRRGVGMFVAAGATDRLRKSGREDFLNHRWPEIAAQIRRLG
                     LSAEELLERTLA"
     gene            complement(597..1175)
                     /locus_tag="VGB57_00560"
     CDS             complement(597..1175)
                     /locus_tag="VGB57_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0129866.1"
                     /translation="MFNDRSSLLSYLATRRSCRPRDLVEPGPDPAQLRQMLEVAARTP
                     DHGKLFPWRFVHVAKDRRAAFAEMLHAAYRAGKPVPPARLEVEAVQRFATQAPELVVL
                     LSTPLQGHKIPVFEQELSCGAVAMNLLHAASALGFGGGWVTGWAAYSAPVLEALGGRP
                     PERVAGFFFLGTGGVELEERPRPVLEQVVKEW"
     gene            1307..2929
                     /locus_tag="VGB57_00565"
     CDS             1307..2929
                     /locus_tag="VGB57_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY0129867.1"
                     /translation="MATTAPSDQPGGYDAKTILGHPRGLFVLFFAEMWERFSYYGMRA
                     LLIFYLTQHWLFSDERSSIIYGAYTALVYITPVLGGYLADRYLGQRKAVLFGAVLLTF
                     GHFLMAFEGNGGQDDTTINIFWLALSFIIVGSGFLKANISVIVGQLYPRTDVRRDGAY
                     TIFYMGINLGAAIGTLIAGYLGQTYGWRYGFGAAGVGMLLGLIVFIWGKPLLMGKGEP
                     RDPARLNNMVGPLKFEWLLYLIGFLAVAGIWVLIQYQEVVGSLLGVFGALLVGYVVFY
                     AVTKLSREDRDRIFAALFLIFGSILFWALFEQAGSSLNLFTDRYVDRAGVPASIFQSI
                     NPIYIILLGPLFAYLWVKLGRAGMEPSAPAKFGFAMLQLGAGFLVLVAGANAAGLDVP
                     TPVIFIFLLYLLHTTGELCLSPVGLSAMNRLAPAHMASLIMGTWFFASATGNFAAGLI
                     AAATGAEGASGEAAGKDTVLDVYSTVGWVAIGVGVLVLVISPLIKKLMHLDTLRDNEN
                     EELAGYKEIGGDRGQDAGMFPHRETKPETKET"
     gene            2944..>3569
                     /locus_tag="VGB57_00570"
     CDS             2944..>3569
                     /locus_tag="VGB57_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0129868.1"
                     /translation="MRLIPWAAVLSATIVSACTPNQKPQTASAQTPRTPPRTYSRDPF
                     PSTYRRYPGAGTAITGATVFDGEGARIDNGVVVLADGVVQAVGGPDTAIPEGATRIDG
                     TGKWVTPGVIDIHSHLGDYPSPSVEAHQDGNEVTSPVRPEVWAEHSVWPQDPGFSRAL
                     VNGGVTSLHVLPGSANLFGGRGVTLKNVYGRTMQQMKFPGAPYSLKMA"
BASE COUNT          552 a         1214 c         1179 g          624 t
ORIGIN      
        1 tccgggcttc gccggaatga cgtaccggga tgacgctatg ccagcgttcg ctccagcagc
       61 tcttcggcgc tgaggccgag gcggcggatc tgggcggcga tttccggcca gcgatggttg
      121 aggaaatcct cgcgtccgct cttgcgcagg cggtcggtgg cgccggcggc gacgaacatc
      181 ccgactccgc ggcggaccag caccagccct tcgtcctgga aggtctgata ggccttggcc
      241 acggtcaggg gattggcgcc gaactcggcg gcgaggctcc tcaccgacgg cagcggatcg
      301 ccgtcgccga cgcgtccctc gaggatcatc gcggcgatgt tgtcgcgcag gcggagatag
      361 actggacgtt cttcggacac ggcgctatag tgagctaata cagtgcggca gtcaagggcg
      421 cctcttgcct ccctgcgccg aaggcgtggg gagggggacc acacgaagtg tggtggaggg
      481 gtaattggct cggaagccga ggttccgcgc gcgatccact tcggcctgcg ccttctaccc
      541 ctccaccatg cgtcgcatgg tccccctccc catctggcga tggggaggac gaagggtcac
      601 cactccttca ccacctgctc cagcacaggc ctcggccttt cctccagctc cacccctccg
      661 gtgcccagga agaagaaccc cgcgacccgt tcgggcggcc gcccgcccag ggcttccagc
      721 accggcgccg aataagccgc ccagcccgtc acccagccgc cgccgaagcc cagggccgag
      781 gcggcgtgga ggaggttcat ggcgacggcg ccgcaggaga gctcctgctc gaaaaccggg
      841 atcttgtggc cctggagcgg ggtggagagc aggacgacca gctccggcgc ctgggtggcg
      901 aaacgctgca ctgcctcgac ctcgagccgc gccggcggga ccggcttgcc ggcgcggtag
      961 gccgcgtgga gcatctcggc aaaggcggcg cggcggtcct ttgcgacatg gacgaagcgc
     1021 caggggaaga gcttgccatg atccggggtg cgcgcggcga cctcgagcat ctgccgaagc
     1081 tgggcggggt ccggcccggg ctcgacgagg tcgcgcgggc ggcacgagcg ccgggtggcg
     1141 aggtaggaga gcagggagga tcggtcgttg aacatcacgc gggggcgcta gcagccttgc
     1201 gggggtgcaa caatcggctt cacatcggcg ttttccccgc tactgtgccc gcgctttcgt
     1261 cggcgcccgt cagaggctgc cgcccagagt ttaggagtag agcctgatgg caaccaccgc
     1321 cccttcggac cagcccggcg gctacgatgc caagacgatc ctcgggcacc cgcgcggcct
     1381 gttcgtgctg ttcttcgccg aaatgtggga gcgcttctcc tattacggca tgcgggcgct
     1441 gctgattttc tacctgaccc agcattggct cttctcggac gagcgaagct cgatcatcta
     1501 cggcgcctat acggccctcg tctacatcac cccggtgctc ggcggctatc tcgccgaccg
     1561 ctatctgggg cagcgcaagg cggtgctgtt cggcgccgtc ctgctcacct tcggccactt
     1621 cctgatggcg ttcgagggca atggcggcca ggacgacacg accatcaaca tcttctggct
     1681 ggccctctcc ttcatcatcg tcggttcggg cttcctcaag gcgaacatct cggtgatcgt
     1741 cggccagctc tatccgcgga cggacgtccg ccgcgacggc gcctacacga tcttctacat
     1801 ggggattaac ctcggcgccg cgatcggcac tctgatcgcc ggctatctcg gccagaccta
     1861 cggctggcgc tacggcttcg gagcggcggg tgtcggaatg ctgctcggcc tgatcgtctt
     1921 catctggggc aagccgctgc tgatgggcaa gggcgagccc cgcgatccgg cgcggctcaa
     1981 caacatggtc gggccgctca agttcgagtg gctgctctat ctgatcggct tcctcgccgt
     2041 cgccggcatc tgggtcctga tccagtatca ggaagtggtc ggaagcctgc tgggcgtgtt
     2101 cggcgcgctg ctcgtcggct acgtcgtctt ctacgcggtg acgaagctgt cgcgcgagga
     2161 ccgcgaccgg attttcgcgg ccctcttcct gatcttcgga tcgatcctgt tctgggccct
     2221 gttcgagcag gcgggctcct cgctcaacct gttcaccgac cgctatgtcg accgcgcggg
     2281 agtgcccgcg tcgatcttcc agtcgatcaa tcccatctac atcatcctgc tcggcccgct
     2341 cttcgcctat ctgtgggtga agctcggccg ggccgggatg gagccttcgg cgccggccaa
     2401 gttcggcttc gccatgctcc agctcggcgc cggtttcctc gtgctcgtcg ccggcgccaa
     2461 cgcggccgga ctggacgtgc cgaccccggt catcttcatc ttcctcctct acctcctcca
     2521 cacgaccggc gagctctgcc tgtctcccgt cggactgtcg gcgatgaacc gcctcgcgcc
     2581 agcccacatg gcgagcctga tcatgggcac ctggttcttc gcctcggcga ccggcaattt
     2641 cgcggccggg ctgatcgccg cggcgaccgg cgccgaaggg gcgtcgggcg aggccgccgg
     2701 caaggatacg gttctcgacg tctattcgac cgtcggctgg gtcgccatcg gggtgggagt
     2761 cctcgtcctc gtcatctcgc cgctgatcaa gaagctgatg catctcgata cgttgcgcga
     2821 taatgagaac gaagagcttg cgggctataa ggagatcgga ggcgatcggg gccaggacgc
     2881 cggcatgttc ccgcatcgcg aaaccaagcc cgaaaccaaa gagacctgac gaaaaggacc
     2941 tcgatgcgcc ttatcccctg ggcggccgtc ctctcggcca ccatagtttc cgcctgtacc
     3001 cccaaccaga agccgcagac ggcgtcggcg cagacgccgc ggactccgcc caggacctat
     3061 tcgagggatc ccttcccctc gacctaccgg cgctatccgg gcgcggggac ggcgatcacc
     3121 ggagccacgg tgttcgacgg cgagggcgcc cggatcgaca acggagtcgt ggtgctggcc
     3181 gacggtgtcg tccaggcggt cggcggcccg gacaccgcca tccccgaagg cgcgacccgg
     3241 atcgacggca ccggcaagtg ggtgactccc ggggtcatcg atatccacag ccatctcggc
     3301 gactatccca gcccctccgt cgaggcgcac caggacggca atgaggtgac cagcccggtt
     3361 cgtccggaag tctgggccga gcacagcgtg tggccgcagg atccgggctt cagccgggcg
     3421 ctggtcaacg gcggggtcac cagcctccat gtccttcccg gttcggcgaa cctgttcggc
     3481 ggccgcggcg tcacgctgaa gaacgtgtac ggccgcacga tgcagcagat gaaattcccc
     3541 ggagcgcctt attcgctgaa gatggcctg
//
LOCUS       DASWNU010000028         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_130549, whole genome shotgun sequence.
ACCESSION   DASWNU010000028 DASWNU010000000
VERSION     DASWNU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130549"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VGB57_00575"
     CDS             complement(<1..531)
                     /locus_tag="VGB57_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0129869.1"
                     /translation="MTAIGAIDSPALAEADVAILGAGPAGCAAAITAASAGARVTLIE
                     ARCFPRAAPGETLPPGAEPLLRQLGLWDEVVASGFHRHEGIWVERNGKARFEAYGADE
                     AGPWRGLQAERSRFDALLLERARQAGAVVLQPARPIGVTIADEVRLRTDAGELRTRWL
                     IDAAGSGHWLARRLGLE"
     gene            complement(528..>1611)
                     /locus_tag="VGB57_00580"
     CDS             complement(528..>1611)
                     /locus_tag="VGB57_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129870.1"
                     /translation="APGIEQQSRAAYVLLSAPDLFPSCGQRELSRWAASNEIPGRFRG
                     KIWGVPPGALCDTRLPANLQLPGQTFDPEEDTVTAVVGMGQRGATPPFARIPDVVRTS
                     SLPDDAAGIFAPGWDVSVDVRDPKGANPTPHLAAYGLGSPFPEDAKLCAALSTFWPAV
                     SPDVYRGLSPHTGNPDLRGSVAPLTDEEIGQSGTLPWDGVPGPRIVQEGGRDWVEMAS
                     FLHADYVHNAAENRFSTRLTARITAEEYQRRVLVAARTHWILSGGMDIAQARPQWLFL
                     SFRQIGSGDPELQEAQKEAGHVLRGQPYRIAACFMGDPTSPMPGNPRLRRMPLQRLNL
                     LFVAASDLRALRRRSNDPEFARVDAE"
BASE COUNT          260 a          549 c          565 g          237 t
ORIGIN      
        1 ctcgagccca agtcgccgcg ccagccaatg cccggacccg gccgcgtcga tcagccagcg
       61 ggtgcgaagc tcgcctgcat ccgtccgaag cctcacctcg tccgcgatcg tcactccgat
      121 gggcctcgcc ggctgaagca cgaccgcgcc ggcctggcgg gcgcgttcga gaagtagcgc
      181 atcgaatcgc gagcgctccg cctgcaggcc ccgccaaggc cccgcttcat ccgcgccata
      241 agcttcgaag cgcgccttgc cgttccgctc cacccagatt ccctcatgcc ggtggaatcc
      301 ggaagcgacg acctcgtccc ataggccaag ctgcctcagc aggggctcgg cgccgggggg
      361 aagggtctcg cctggcgccg cgcgcgggaa gcaacgcgcc tcgatcaggg ttactcgagc
      421 tccggccgat gccgcggtga ttgcggccgc gcagccagcg ggtccagcgc cgagaatggc
      481 gacatcggcc tcggccaaag cgggactgtc gatcgcgccg atcgccgtca ctcggcgtcg
      541 acccgggcaa attccgggtc gttgctccgc cggcgaagag cgcgaagatc gctcgccgcg
      601 acgaagagaa ggttgaggcg ctggagcggc atccgtcgaa gcctcggatt gccaggcatc
      661 ggcgaggttg ggtcgcccat gaagcaggct gcgatgcgat aaggctggcc gcgaaggacg
      721 tggccggctt ccttctgcgc ctcctgaagc tccggatcgc ccgagccgat ctgccggaag
      781 gagaggaaca gccattgcgg ccgcgcctgg gcgatgtcca tgccgcccga caagatccag
      841 tgcgtgcggg ctgcgaccag cacgcgcctt tgatattcct cggccgtgat ccgggcggtg
      901 agcctcgtgc tgaagcggtt ctcggccgcg ttgtggacat agtcggcatg aaggaagctt
      961 gccatctcga cccagtcgcg gccgccctcc tgaacgatcc ggggccccgg aacgccgtcc
     1021 cagggtaggg tgccgctctg cccgatctcc tcgtcggtca gcggagccac cgatcctctt
     1081 aggtccggat tgccggtatg cggcgagagg ccgcgataga catcgggaga gaccgccggc
     1141 cagaaggtgc tgagcgcggc gcacagcttt gcatcctccg ggaaggggct gcccaggcca
     1201 taagcggcga ggtgcggtgt gggattggca cccttgggat ctcgcacgtc gaccgagaca
     1261 tcccagcccg gcgcgaagat tccggccgca tcgtcgggca atgaggaggt ccgcaccacg
     1321 tcggggatcc gggcgaaggg cggggtcgct ccgcgctggc ccatgcctac caccgccgtg
     1381 acggtatcct cctcgggatc gaaggtctgg cccggcagct ggaggttggc cgggaggcgg
     1441 gtgtcgcaca aggcgcccgg cgggacgccc cagatcttgc cccggaagcg acccggaatc
     1501 tcattggagg cggcccagcg cgagagctcg cgctggccgc aggacgggaa caggtcgggc
     1561 gcgctcaaaa gcacgtaggc cgcgcgtgat tgctgttcga tcccaggcgc g
//
LOCUS       DASWNU010000029         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_363817, whole genome shotgun sequence.
ACCESSION   DASWNU010000029 DASWNU010000000
VERSION     DASWNU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363817"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="grpE"
                     /locus_tag="VGB57_00585"
     CDS             complement(<1..512)
                     /gene="grpE"
                     /locus_tag="VGB57_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0129871.1"
                     /translation="MNEDQELHEETSETEDGAAREQAPEQDLSELVKELEQVRQHVLY
                     AQAETQNVRRRLEQEKQTVAAYAATGFARDVLSVKDNLDRALAAIPEDLRQDERMKSL
                     IAGIEATGRELDTVFQRNGITRVEALGQPLDPHRHQAMVEIPSDDAEPGTIVQEMQAG
                     YMIKDRLLRPA"
     gene            complement(509..1552)
                     /gene="hrcA"
                     /locus_tag="VGB57_00590"
     CDS             complement(509..1552)
                     /gene="hrcA"
                     /locus_tag="VGB57_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0129872.1"
                     /translation="MASTPVSELTERAREVFRLVVENYLGSGAPVGSRTISKLPGLNL
                     SPASIRNVMQDLEELGLLAHPHTSAGRVPTESGLRMFVDGMMQAAEPSAEERAAIEAR
                     LERGGPIEEALSAATAALSGLSACAGIVLVPKREPVLRQLGFVQLSPSQALAVMVGAE
                     GSVENRVVDLPPGITASALTEVGNYVSARLSGLTLPEAQRRLAAEIQAGKDALDDAAR
                     QLIDRGLALWSEDGAQRPVLIVRGQANLLEPGAAADLERVRQLLEELDGKEEIVRLLD
                     SARAGQGMKIFIGSENKLFALSGSSVIAAPYRGRDGKVVGVVGVIGPTRLNYARVVPM
                     VDFTAQALSRLMA"
BASE COUNT          237 a          579 c          562 g          272 t
ORIGIN      
        1 gccggccgca gcagccggtc cttgatcatg tagcctgcct gcatctcctg gacgatggtg
       61 ccgggctcgg cgtcgtcgct ggggatctcg accatcgcct ggtggcggtg ggggtcgagg
      121 ggctggccga gggcttcgac ccgggtgatg ccgttgcgct ggaacaccgt gtcgagctcg
      181 cgcccggtcg cctcgatgcc cgcgatcagg gacttcatcc gctcgtcctg gcgaaggtct
      241 tcggggatcg ccgccagggc gcggtcgaga ttgtccttca ccgacaatac gtcgcgggcg
      301 aagccggtcg ccgcataggc ggccacggtc tgcttctcct gctcgagccg gcggcggacg
      361 ttctgggtct ccgcctgcgc gtagaggacg tgctggcgga cctgctccag ctccttgacc
      421 agctccgaca aatcctgctc cggcgcctgc tcccgggcgg cgccatcctc ggtctcgctg
      481 gtctcttcgt gcagttcctg gtcttcgttc atgccatcaa tctcgatagt gcctgtgccg
      541 tgaaatccac catgggtacg acccgcgcgt agttcaaccg cgtcggcccg atcaccccta
      601 ccactccgac caccttgccg tcgcggccac gataaggcgc ggcaatgacg gaagatccgg
      661 acaaagcgaa caatttgttc tccgagccga tgaagatctt catcccctgg cccgcccggg
      721 cgctgtcgag caggcggacg atctcctcct tgccgtccag ctcctcgagc agctggcgga
      781 cgcgctcgag gtccgcggcg gcgcccggct cgagcagatt cgcctggccc cgcacgatca
      841 gcaccggccg ctgggcgccg tcctcggacc acaaagccag gccccggtcg atcagctgcc
      901 gggcggcatc gtcgagcgcg tccttgccgg cctggatctc ggcggcgagg cgccgctgcg
      961 cttccggcag ggtgagaccg gacaggcggg cgctgacgta attgccgact tcggtgagcg
     1021 ccgaggcggt gatccccggc ggcaggtcga cgacccggtt ctcgacgctg ccctcggctc
     1081 cgaccatcac cgccagcgcc tggctcggcg acagctggac gaagcccaat tgccgaagca
     1141 ccggctcgcg cttcggcacc aggacgattc cggcgcaggc ggagagaccg gacagggcgg
     1201 cggtggcggc ggacagcgcc tcctcgatcg gaccgccgcg ctccagccgc gcctcgatcg
     1261 ccgcgcgctc ttcggcgctt ggctcggccg cctgcatcat cccgtcgacg aacatcctga
     1321 gcccgctctc ggtggggacc cgcccggccg acgtatgggg atgggcgaga aggccgagct
     1381 cctccagatc ctgcatgacg ttgcggatcg aggccggcga caggttgagg ccgggcagct
     1441 tggagatcgt acgcgacccg accggagcgc ccgagccgag ataattctcg accaccagcc
     1501 ggaaaacttc ccgggcgcgt tcggtgagct cgctgacggg ggtggaggcc atgggggcta
     1561 tgtaggggag tcgtcatccc ggcgaaagcc gggacctcgg tgagacccgc cgagactatt
     1621 cgaccccggg gtcccggctt tcgccgggat
//
LOCUS       DASWNU010000030         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_493684, whole genome shotgun sequence.
ACCESSION   DASWNU010000030 DASWNU010000000
VERSION     DASWNU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493684"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VGB57_00595"
     CDS             <1..331
                     /locus_tag="VGB57_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HEY0129873.1"
                     /translation="LYRQVDAERSGFDDSADPEPLGLAQRAEIEREARRYADALGYSV
                     DWEAVTRLDDEMLVNAIAQVAPFDVGSKQALLEAPDIAERCDLLVQFMQFQQMVPGGA
                     EGPETLQ"
     gene            340..525
                     /locus_tag="VGB57_00600"
     CDS             340..525
                     /locus_tag="VGB57_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY0129874.1"
                     /translation="MLALLVCPVTRTALKWDKERQELVSEAAGVAYPVRDGVPVMLIE
                     EARILPPHAEHGEGDQA"
     gene            complement(650..1858)
                     /locus_tag="VGB57_00605"
     CDS             complement(650..1858)
                     /locus_tag="VGB57_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0129875.1"
                     /translation="MDRADVIILGGGLIGLTLAVALDRHGLSTVVVDPADPESNLAPA
                     FDGRATAVASASWRMLEAIGVAGRIEDEGCPILSIRVSDGLQPGGIAFDSPDPDDPLG
                     IMVENRRLRQVLREAALEAPRVKLLMPARAADIVRDSAGVRVALEDGRAVAGALLVGA
                     EGRSSPTREAANIPLARWSYDHAAVVLTVEQERHHENAAYEIFYPTGPFAVLPLLPGT
                     RSAIVWSVPAADAAAVIDLPDRAMAHEIAKRMGGFLGEVRLAGPRWNYPLGFHHAATI
                     TAERLALAGDAAHGIHPIAGQGLNLGFRDVAALTQVLVEGARLGLDPGDAQSLSRYER
                     WRSLDTLTVAAATDALTRIYGVPGRTASAIRRFGMGIVQRVGPLKSQFMAEARGESGE
                     LPLLLRGLTI"
     gene            1974..>3902
                     /locus_tag="VGB57_00610"
     CDS             1974..>3902
                     /locus_tag="VGB57_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY0129876.1"
                     /translation="MAASAAAQSARQVRLAQWRTGMKNGMRRSWALLIGGAIGAGALL
                     MLLALVSYEPTDPSLNTAAAGPVRNWVGAPGAWIADLLLSLFGPPVALLLPLIVLIGV
                     RIATGAPSTRWPRSLGLTLLGLTLVGGAASLLVGEAVNGLPASWGGAAGLSLAKLVEL
                     GLAAIGEPGIVEPFKIVTVGLFGALGLASFWFGLGLRSDERTWIASRRLPRRERALAD
                     DSDELPEPQDRPARTPIIAPAEPPRTVINDRIRTPAQAKRPLRERQASLALGDSYQLP
                     GLELLSPAPPPVGGALDKASLERNARLLESVLDDFSVKGEIVEVRPGPVVTMYELEPA
                     SGIKASRVIQLADDIARNMSALSARVATIPGRSVIGIELPNPKREMVVLSELIASHAF
                     EDQVAQLPIVLGKNIAGDPVVADLAPMPHLLVAGTTGSGKSVGLNCMILSLLYRLTPE
                     QCKMILIDPKMLELSSYDDIPHLLAPVVTEPAKAIRALKWTVEQMEERYRMMASVGVR
                     QLSSFNAKVRESKAKGQSMGRRVQTGYDPETGQPKYEMEELDYEVLPQIVVVVDELAD
                     LMMTAGKEVEFLIQRLAQKARAAGIHLIMATQRPSVDVITGVIKANLPTRISFAVTSK
                     IDSRTILGEQGAEQLLGKG"
BASE COUNT          604 a         1338 c         1380 g          580 t
ORIGIN      
        1 gctctaccga caggtcgacg ccgagcgtag cggcttcgac gattccgccg acccggagcc
       61 gctcggcctc gcccagcgcg ccgagatcga gcgggaggcg cgccgctatg ccgacgcgct
      121 cggctattcg gtcgactggg aagccgttac ccggctcgac gacgagatgc tggtcaacgc
      181 catcgcccag gtggcgccgt tcgacgtcgg ctcaaagcag gccctgctcg aggcgcccga
      241 catcgccgag cgctgcgacc tgctcgtcca gttcatgcag ttccagcaga tggtcccggg
      301 cggagcggaa ggtccggaga ccctgcagtg acggaggaga tgctggcact gctggtctgc
      361 ccggtcacac ggactgcgct gaagtgggac aaggagcgac aggagctggt gtcggaagcg
      421 gccggggtgg cctatccggt tcgggacggc gtgccggtga tgctgatcga ggaagcgcgt
      481 attttgcctc cccatgcaga gcatggggag ggggaccagg cgtagcctgg tggaggggtg
      541 gaaggcgccg ggcgaggagg ttctcgcgtg aaccgcagga atctgcggca gttacccctc
      601 caccacactt cgtgtggtcc ccctccccat ctggcgatgg ggaggcaaat caaatcgtca
      661 ggcccctcag gagcagcggc agctcgccgc tctctccccg ggcttcggcc atgaactggc
      721 tcttgagcgg gccgacgcgc tggacgatgc ccatcccgaa tcggcggatc gccgaggccg
      781 tgcggccggg cactccgtat atccgcgtca gggcgtcggt ggcggcggcg actgtgaggg
      841 tgtcgaggct gcgccagcgc tcatagcggg agagagactg ggcgtcgccg gggtcgaggc
      901 ccaggcgggc gccttcgaca aggacctggg tgagcgcggc gacgtcgcgg aagccgaggt
      961 tgaggccctg ccccgcgatc ggatggatcc cgtgggcggc gtctccggcc agagccagcc
     1021 gctccgcggt gatggtcgcg gcgtgatgaa agccgagcgg atagttccag cgcggcccgg
     1081 cgagccgcac ctcgccgagg aagccgccca tcctcttggc gatttcgtgg gccatggccc
     1141 ggtccggcag gtcgatcacc gccgcggcat cggccgccgg aaccgaccag acgatcgcgg
     1201 atcgagtgcc gggcaacagc ggcaggacgg cgaacggacc cgtcggatag aaaatctcgt
     1261 aggcggcatt ttcgtgatgc cgctcttgct cgacggtgag caccaccgcc gcatggtcgt
     1321 agctccagcg ggcgagcggg atgttggccg cctcccgggt gggggagctg cggccctcgg
     1381 cgccgaccag caatgcccct gcaacggcgc ggccgtcctc gagcgcgacc cgcaccccgg
     1441 ccgaatcgcg gacgatgtcg gccgcccgcg ccggcatcag cagcttcacc cggggcgcct
     1501 cgagcgccgc ctcgcgaagc acctgccgaa gccgcctgtt ttccaccatg attccgagcg
     1561 gatcgtcggg atcgggcgag tcgaaggcga ttccgcccgg ctgaagcccg tcgctgacgc
     1621 ggatggagag gatcgggcag ccttcgtcct cgatccgccc ggccacgccg atcgcctcca
     1681 gcatccgcca cgaagcgctt gcgacggcgg tcgcccggcc gtcgaaggcc ggggcgaggt
     1741 tgctctcggg gtcggcggga tcgacgacca cggtcgagag gccgtggcgg tcgagagcga
     1801 cggcgagggt caggccgatg agcccgccgc ccaggatgat gacgtccgca cgatccatgt
     1861 tcagccttcc agatgatttt cccgccctac cgatgcaaag ctgcaacgcc aagcgcttga
     1921 ccgcggactc ccgaggggcg cattgtcgcg ctcggaacat ttggggcacg ggaatggcgg
     1981 cttcagcagc ggcgcagagc gcgcgccagg tgagactggc gcagtggcgg accggaatga
     2041 agaacggcat gcgccggagc tgggcgctgc tgatcggcgg agcgatcggc gccggggccc
     2101 tgctcatgct tctggcgctg gtcagctacg agccgaccga cccttccctc aacaccgccg
     2161 cggcgggccc ggtccggaac tgggtcggcg ctccgggggc ctggatcgcc gaccttctgc
     2221 tgagcctgtt cggaccgccg gtggcgctgc ttctgccgct gatcgtcctg atcggggtcc
     2281 ggatcgcgac cggcgccccg tccacccgct ggccgcgctc gctcggcctc accctgctcg
     2341 ggctgaccct ggtcggaggc gccgcctcgc tgctggtcgg cgaagcggtc aacgggctgc
     2401 cggcgagctg gggcggagcg gccggcctct cgctcgccaa gctcgtcgag ctcggcctcg
     2461 cggcgatcgg cgagcccggg atcgtcgagc cgttcaagat cgtcacggtc ggccttttcg
     2521 gagcgctggg cctggcctcg ttctggttcg gcctcgggct gcgctccgac gagcggacct
     2581 ggatcgcctc gcgccgcctg ccgcggcgcg agcgcgcgct cgccgacgac agcgatgagc
     2641 ttccggagcc gcaggatcgg ccggcgcgca cgccgatcat cgcaccggcc gagccgcccc
     2701 gtaccgtcat caacgaccgg atccgcactc cggctcaggc gaagcggccg cttcgcgagc
     2761 gccaggcgtc gcttgccctg ggcgacagct atcagctgcc cgggctcgag ctgctctcgc
     2821 cggccccgcc gccggtcggc ggagcgctcg acaaggcgag cctggagcgc aatgcccggc
     2881 tgctcgaatc ggtgctcgac gacttctcgg tcaagggcga gatcgtcgag gttcggccgg
     2941 gcccggtcgt caccatgtac gaactggagc cggcgagcgg gatcaaggcg agccgggtga
     3001 tccagctcgc cgacgacatc gcccgcaaca tgtccgccct ttccgcccga gtggccacca
     3061 tcccgggccg gagcgtgatc gggatcgaat tgcccaaccc gaagcgggag atggtggtcc
     3121 tttcggagct gatcgcgagc cacgccttcg aggaccaggt cgctcagctt ccgatcgtgc
     3181 tcggcaagaa catcgccggg gaccccgtcg tcgccgatct ggcgccgatg ccccatctgc
     3241 tcgtcgccgg caccaccggc tcgggcaagt cggtcggcct caactgcatg atcctgtcct
     3301 tgctctaccg gctgacgccc gagcagtgca aaatgatcct catcgacccg aagatgctcg
     3361 agctgagcag ctacgacgac atcccgcatc tgctcgcgcc ggttgtgacc gagcccgcca
     3421 aggcgatccg cgccctgaag tggacggtcg agcagatgga ggagcgctac cggatgatgg
     3481 cctcggtcgg ggtccgccag ctcagcagct tcaacgccaa ggtgcgcgaa tcgaaggcca
     3541 agggccagtc gatggggcgg cgggttcaga ccggctacga tccggagacc ggccagccca
     3601 aatatgagat ggaggagctc gactatgagg tccttcccca gatcgtcgtc gtcgtcgacg
     3661 agctcgccga tctgatgatg actgccggca aggaggtcga gttcctgatc cagcgcctcg
     3721 cccagaaggc gcgcgcggcg ggcatccacc tgatcatggc gacccagcgc ccgtccgtcg
     3781 acgtcatcac cggagtgatc aaggcgaacc tcccgacccg gatcagcttc gcggtcacct
     3841 cgaagatcga cagccggacc atcctcggcg agcagggcgc cgagcagctg ctgggcaagg
     3901 gc
//
LOCUS       DASWNU010000031         7357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_467951, whole genome shotgun sequence.
ACCESSION   DASWNU010000031 DASWNU010000000
VERSION     DASWNU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467951"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VGB57_00615"
     CDS             complement(<1..189)
                     /locus_tag="VGB57_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129877.1"
                     /translation="MLSLSFVRENREIVEKAIREKNVALDLDRLLALDGEVRGLKTRI
                     DELRRRRNEISAGFKTADP"
     gene            complement(231..878)
                     /locus_tag="VGB57_00620"
     CDS             complement(231..878)
                     /locus_tag="VGB57_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129878.1"
                     /translation="MEVVGSYEEHGYAHVRGLVEPRVAQAFLHRIAKDLGRTALPLRG
                     KDAQPGVLKRPALQVFSADYAPMQFFLFGLTPLMSRLVGRELLPTYDYFRVYRAGDIC
                     RVHSDRDACEHSLSLTLDYSDGKVWDLEIGSRALPGRQKLLTEDFGDETYASVAMAPG
                     DAVLYRGIHRRHGRMTPNPNRWSAHLFLHWVEAEGEYRDQAFQGAEPVTGKVSFG"
     gene            994..1086
                     /locus_tag="VGB57_00625"
     tRNA            994..1086
                     /locus_tag="VGB57_00625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1028..1030,aa:Ser,seq:gct)
     gene            1269..2048
                     /locus_tag="VGB57_00630"
     CDS             1269..2048
                     /locus_tag="VGB57_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HEY0129879.1"
                     /translation="MMRAVFVRLAAAAALALAAAAAPAQVATVDPNSAVDSAPPAEAA
                     QPGWESDEPEYQPVDSGAQEAAAGNQAAVEASAREDAAAAPAGTASQRASQAAKGETI
                     GLKRVLGAAEGVFGKGAEGLADLIEDVLRDRGDPTGYITGREAGGAFIAGVRYGSGML
                     HHQVEGDRKVHWTGPSVGFDFGANADKVFVLVYNLHDSERLFRRFPAGEGKAYVVGGF
                     TASYLRRGDVVLIPIRLGVGARLGVNAGYMRFSQKSRWLPF"
     gene            complement(2189..2443)
                     /locus_tag="VGB57_00635"
     CDS             complement(2189..2443)
                     /locus_tag="VGB57_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129880.1"
                     /translation="MASFSLEIQEESFAHDPQHRGYLVAYRENEVNHCPGCGRTHWYV
                     GRVSAECGFCATALPLISATSRGAGTHSRGHRPMALSEAA"
     gene            complement(2590..4188)
                     /locus_tag="VGB57_00640"
     CDS             complement(2590..4188)
                     /locus_tag="VGB57_00640"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEY0129881.1"
                     /translation="MREFSQLLDGLVYTRSRNGKLKLIGDYLKRTPDPDRGYALAALT
                     GEIDLPGVKPAAIRAIVEERVDPVLFHMSWDYVGDMAETVSLLWPKRIDQPAEIDDGS
                     LRIASVVERLQTLGRAEAPGALARMLDHLDASGRYALLKLATGELRIGVSARLAKTAL
                     AQAFGLDVEAVEEVWHGIDPPYLPLFDWAEGRGEQPTAENVPVFRPFMLAHPLEDLRL
                     SLADYAAEWKWDGIRVQIVRAGGETRLYSRAGDDISRSFPEVAESFRADGAVDGELLV
                     KGEFQGADAHGGAAASFNALQQRLGRKLVSARMLKEYPAFVRLYDILFDGPEDLRALS
                     WEERRARLATFVPRLDPERFDLSALIEATTFEELEEIRAGTRDAAIEGVMLKRRDSPY
                     TGGRRVGLWYKWKRDPLTADCVLMYAQRGSGKRSSYYSDFTFGCWTPEGELLPVGKAY
                     FGFTDEELLWLDRFVRNKTVKRFGPVREVEKSLVLEVAFDSIHSSKRHKSGLAMRFPR
                     ISRIRTDKPAAEADTIDGLLRLVT"
     gene            complement(4305..4871)
                     /locus_tag="VGB57_00645"
     CDS             complement(4305..4871)
                     /locus_tag="VGB57_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0129882.1"
                     /translation="MYHPDLLRHPDACPALVLNADYTPLSYYPLSLWPWQTAVKAVFL
                     ERVDIVAQYVREVHSPTRTLQLPSVIALRQYVKPSEHPAFTRFNLFLRDRFECQYCAS
                     PSELTFDHVVPRAQGGRTTWENVATACAPCNLRKGGRTPLQAHMNLNRRPYRPTSWQL
                     QENGRSFPPGYLHESWRDYLYWDIELEA"
     gene            5020..5886
                     /gene="gluQRS"
                     /locus_tag="VGB57_00650"
     CDS             5020..5886
                     /gene="gluQRS"
                     /locus_tag="VGB57_00650"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY0129883.1"
                     /translation="MKLVTRFAPSPTGRLHLGHAFSALLARDFARSRGGAFLLRIEDI
                     DPGRSRPEHVDSIIEDLAWLGLEWDGEIIFQSERLGLYAEALDRLKAAGLVYPCFCTR
                     AAIAAEIAASAAAPHGPGGPLYPGTCRALTETERERRCTGEPHAWRLDVAKAVAVAGP
                     IYWRDGDTEVQAEPEAHGDVVLARKDAPVSYHLAVTVDDSAQGVTDVVRGRDLYSATD
                     VHRLLQALLGLPTPLYHHHPLLLGPDGRRLAKRHGAPTLRELREGGVDPKSLAADLLT
                     GQMPYGYSAADT"
     gene            5896..6099
                     /locus_tag="VGB57_00655"
     CDS             5896..6099
                     /locus_tag="VGB57_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIG1 domain-containing protein"
                     /protein_id="HEY0129884.1"
                     /translation="MTTILIILIVLMAGATLFVLAKGVIGMAQGKDITGERSQALMRK
                     RVLYQAIAIVLVVLLLLVASGKS"
     gene            6103..6672
                     /locus_tag="VGB57_00660"
     CDS             6103..6672
                     /locus_tag="VGB57_00660"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0129885.1"
                     /translation="MVRLNQIYTRTGDSGASGLTDGSRRSKADPLFAAIGDVDEANSV
                     IGVAVLEVADEARRRMLTAIQNELFDLGADLATPGEDFAPSEMTLRIVQPQIDRLELE
                     IDSMNEGLEPLRSFILPGGGGGAAAIHLARAIVRRAERSAIAASEATPLNPLALIYLN
                     RLSDHLFVMARVAAAGEGGEVLWKPGATR"
     gene            6804..>7357
                     /locus_tag="VGB57_00665"
     CDS             6804..>7357
                     /locus_tag="VGB57_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0129886.1"
                     /translation="MATQANSPPALALAQRYRAVRALSEAIAAPLSDADSTLQPMPDA
                     SPAKWHLAHTSWFFETFVLRDHVPGHRLYDESWAYLFNSYYEGEGERHPRDRRGMLSR
                     PSLEEVRAWRAYVDEAVAAALPRLPEDALALVELGLNHEQQHQELMLMDLTAALAETP
                     LRPPLWAGTAPAPAALAPAMEWIEG"
BASE COUNT         1170 a         2528 c         2469 g         1190 t
ORIGIN      
        1 cgggtcggcg gtcttgaagc cggcgctgat ctcgttgcgc cgcctgcgca gctcgtcgat
       61 ccgggtcttg agcccgcgga cctcgccatc gagcgcgagc agccggtcca ggtccagggc
      121 gacgttcttt tctcggatcg ccttttcgac gatctccctg ttctcgcgga cgaagctgag
      181 gctgagcatg tgcggcgcct agcgcccaat cggctggaag ccaaccaggg tcagccgaaa
      241 ctcaccttgc cggtaacggg ctcggcgccc tggaaagcct gatcgcgata ctcgccctcg
      301 gcctccaccc aatgcaggaa aaggtgggcg gaccagcggt tcgggttcgg ggtcatgcgc
      361 ccgtggcggc ggtggatgcc gcggtagagg acggcgtctc caggggccat tgcgaccgag
      421 gcgtaggtct cgtcgccgaa atcctcggtc aggagcttct ggcggccggg aagcgcgcgg
      481 ctgccgatct cgaggtccca gaccttgccg tcgctatagt cgagggtaag cgaaaggctg
      541 tgctcgcagg cgtcgcggtc ggaatggacg cggcagatgt cgccggcgcg gtagacccgg
      601 aaatagtcgt aggtgggaag gagctcgcgg ccgacgagcc ggctcatcag gggagtcagg
      661 ccgaagagga agaattgcat gggcgcataa tcggcgctga aaacctggag ggccggccgc
      721 ttcaggaccc cgggctgggc atccttgcct cgaagcggaa gcgccgtcct gccgagatcc
      781 ttggcaatcc gatgcaggaa ggcctgcgcg acccgaggct cgaccagccc cctgacatgc
      841 gcgtagccat gctcctcgta gctgccgacg acctccatgg cgccgggcta ccgccccgcc
      901 gcggagggtg caagggcggg gacgggcgac aagccttcac ggctcggttg aacgattgga
      961 ttcccgccta tatagggcgt cgcttgccgc gccggagacg tgggtgagtg gctgaaacca
     1021 gcggtttgct aaaccgccgt agggggatta ctcctaccga gggttcgaat ccctccgtct
     1081 ccgccagcct gtgggaattg ccgcccttcc ccggccggag tcctacggta gccgtgcttc
     1141 cgtgagcctg aaaatgcggt ccgcccaccg gtccggcgcg ccagcgactc ggctcgtcgc
     1201 ccgttcattg cggcgacagc cttgaaagca tttacttccc cgcaggagcg tcagggggag
     1261 tttaggacat gatgagggcc gttttcgtga ggcttgcggc cgccgccgct ttggctctcg
     1321 ccgccgccgc ggcgccggcg caggtcgcca ccgtcgatcc caacagcgcc gtcgattcgg
     1381 cgccgcccgc cgaggccgcc cagccgggct gggagtcgga cgagcccgaa tatcagccgg
     1441 tcgactcggg cgcgcaggaa gcggcggccg gcaaccaagc ggccgtcgag gcaagcgcgc
     1501 gcgaagacgc cgccgccgcc ccggccggca ccgcgagcca gcgcgccagc caggcggcca
     1561 agggcgagac catcgggctc aagagggtgc tgggcgccgc cgagggcgtg ttcggcaagg
     1621 gcgccgaagg cctggcggac cttatcgagg acgtgcttcg cgaccgcggc gatcccaccg
     1681 gttacattac cggccgggag gcgggcggcg ccttcatcgc cggggtgcgc tacggctcgg
     1741 gcatgctcca ccaccaggtg gagggcgacc gcaaggtcca ctggaccggg ccctcggtgg
     1801 gtttcgattt cggagccaat gcggacaagg tgttcgtgct tgtctacaac ctccacgaca
     1861 gcgagcgatt gttccgccgc tttccggccg gggagggcaa ggcctatgtc gtcggcggct
     1921 tcaccgccag ctacctgcgg cgcggcgacg tcgtcctcat cccgatccgc ctcggcgtcg
     1981 gagcccggct cggggtcaat gccggctaca tgcgcttcag ccagaagagc cgctggcttc
     2041 cgttctgacc tgaaaatcct cccctgcgtt tgcaggggag ggggaccagg cgaagcctgg
     2101 tggaggggcc ttgcggagtc cgaagaaccc ctccaccgcg catcgcgcgg tccccctccc
     2161 cgcgaaatcg cggggaggat ttttctcgct aggccgcttc gctcagcgcc atcggccgat
     2221 gcccgcggct gtgggtgccg gcgccgcggc tggtcgcgct gatcagcggc aaggcggtgg
     2281 cgcagaagcc gcattcggcc gagacccggc cgacatacca atgagtccgc ccgcagccgg
     2341 ggcaatggtt gacctcattc tcgcggtagg cgacgagata gccgcggtgc tgcgggtcgt
     2401 gcgcgaaact ctcttcctga atctcgagac tgaaactggc catgcccttt atcccttcaa
     2461 aaggggcgga acggtttgca ggcgctccac ctagcctgcc cctgcgacgg cccggcgact
     2521 ctgtcagccc ccgaaaatcg gccgttccaa aacgggaaaa ggcaggccca aaagttaatt
     2581 gaccccgcct tacgtcacca gccgcaatag cccgtcgatc gtgtcggcct cggcggcggg
     2641 cttgtcggtg cggattcgcg agatccgggg gaagcgcatg gcgaggcccg atttgtggcg
     2701 cttgctggag tggatcgaat cgaaggcgac ctcgaggacg aggcttttct cgacctcgcg
     2761 gaccggcccg aaccgcttca ccgtcttgtt tcggacgaag cggtcgagcc agagcaattc
     2821 ctcgtcggtg aatccgaaat acgcctttcc gaccggaagc agctcgccct ccggggtcca
     2881 gcatccgaac gtgaaatcgg aataataaga ggagcgcttg ccgctccccc gctgggcgta
     2941 catcaggacg cagtcggcgg tgagcggatc gcgcttccat ttgtaccaga ggccgacccg
     3001 gcgcccgccc gtataggggc tgtcgcggcg cttcagcatc actccctcga tggccgcgtc
     3061 ccgggtgccg gcgcgtatct cctccagctc ttcgaaagtc gtggcctcga tcagggcgga
     3121 gaggtcgaac cgctcgggat cgagcctggg aacgaaggtt gccagccgcg cccggcgttc
     3181 ctcccaggac agggcccgca agtcctccgg accgtcgaag aggatgtcgt agagccggac
     3241 gaaggcggga tattccttaa gcatcctggc ggaaaccagc tttcggccca gcctttgctg
     3301 gagcgcgttg aagctcgccg ccgctccgcc atgggcgtcc gcgccctgga actcgccctt
     3361 gaccagcaat tcgccgtcga cggctccgtc ggcccggaag ctctcggcga cctcggggaa
     3421 gctccggctg atatcgtcgc ccgcccggct gtagagccgg gtctcgccgc cggcgcggac
     3481 gatctggacc cggatcccgt cccatttcca ctcggccgca taatcggcga gcgacaggcg
     3541 cagatcctcg agcgggtggg cgagcatgaa cggccggaag acgggcacgt tctccgccgt
     3601 cggctgctcg ccgcgccctt ccgcccagtc gaagagcgga agatagggcg ggtcgatgcc
     3661 gtgccagacc tcctcgaccg cctcgacgtc gaggccgaaa gcctgcgcca gagcggtctt
     3721 ggccagccgc gccgagactc cgatccgaag ctctccggtc gccagcttga gaagggcgta
     3781 gcggccgctg gcgtcgagat ggtcgagcat ccgcgccagc gctccggggg cttcggcccg
     3841 gcccagggtc tgaagccgct cgacgacgct ggcgatgcgc agggacccgt cgtcgatctc
     3901 ggcgggctgg tcgatcctct tgggccagag cagcgatacc gtctcggcca tgtcgccgac
     3961 atagtcccag ctcatgtgga agagcacggg atcgacccgt tcctcgacga tggcccggat
     4021 cgccgccggc ttgacgccgg gcaggtcgat ctcgccggtc agcgccgcca gcgcgtagcc
     4081 gcgatcggga tcgggcgtgc gcttcagata atcgccgatc agcttcagct tgccgttgcg
     4141 cgagcgggtg tagacgagac cgtcgagcaa ctgtgagaat tcgcgcatcg ctggcgtaac
     4201 gatttggcgg tggcgggttt cccgtcatcc cggcgaaagc cgggacatcc ggacgagaag
     4261 tctcggcggg tctcgccgag gtcccggctt tcgccgggat gacgctaagc ctccagctcg
     4321 atgtcccaat agaggtaatc gcgccagctc tcgtgcagat agcccggcgg gaagctgcgt
     4381 ccattctcct gcagctgcca gctggtcggc cggtaggggc ggcgattgag gttcatgtgc
     4441 gcctggagcg gagtgcgccc gcccttcctc aggttgcacg gcgcgcaggc ggtggcgaca
     4501 ttctcccagg tcgtcctccc gccctgggcg cggggcacga catggtcgaa ggtgagctcc
     4561 gagggagaag cgcaatattg gcattcgaaa cggtcgcgca ggaacaggtt gaagcgggtg
     4621 aaggcgggat gctcgctcgg cttcacatat tggcggagcg cgatcaccga gggcaattgc
     4681 agggtgcgcg tcgggctgtg cacctcgcgg acatattggg cgacgatgtc gacccgctcg
     4741 aggaacacgg ccttgaccgc cgtctgccac ggccacaggc tcagcggata atagctcaag
     4801 ggcgtataat cggcgttgag cacgagcgcc gggcaggcat cgggatggcg gaggaggtcg
     4861 ggatggtaca tgcgccgcgc gtcgccctcg tccatgcgag gagaagcgcc cggcctgtcg
     4921 cactcagaac gggggagccc tccccagtcc aagccccctc ccatgggaag gtgacggcgc
     4981 catgacagca tggcgcgact cccccggtca agagtccaga tgaagctggt cacgcgtttc
     5041 gctccgagcc cgaccgggcg gttgcatttg ggccacgctt tctcggccct gctcgctcgc
     5101 gatttcgcgc gcagccgagg cggcgccttc ctgctcagga tcgaggatat cgacccgggc
     5161 cgttcccgcc ccgagcatgt cgactcgatc atcgaggacc tcgcctggct cggcctcgaa
     5221 tgggacgggg agataatctt ccagtcggag cggcttgggc tctatgccga ggcgctcgac
     5281 cggctgaagg ccgccggcct cgtctatccc tgcttctgca cccgcgccgc catcgccgcc
     5341 gagatcgcgg ccagcgccgc cgcccctcat ggtcccggcg gccccctcta tccgggcacc
     5401 tgcagggctc tcaccgagac agagcgcgag cggcgctgca ccggtgagcc gcacgcctgg
     5461 cgcctcgacg tggcgaaggc ggtggccgtc gccggcccga tctactggcg cgacggcgac
     5521 accgaggtcc aggccgagcc cgaggcccat ggcgacgtcg tcctcgcccg caaggacgct
     5581 ccggtcagct atcatctcgc cgtcaccgtc gacgattccg cgcaaggcgt caccgatgtg
     5641 gttcgaggcc gcgacctcta ttccgcgacc gatgtccatc gcctgctcca ggccctgctc
     5701 ggcctgccga cgccgctcta ccaccaccat cccctgctgc tcggccccga cggccgccgc
     5761 ctcgccaagc gccacggcgc tcccactctg cgcgagcttc gcgaaggggg cgtcgacccc
     5821 aaatcgctgg cggcggacct gctcacggga caaatgccgt atggatattc ggccgcggat
     5881 acctagatag gcgcgatgac cacaatcctc atcatcctca tcgtcctcat ggccggagcg
     5941 acgctcttcg tcctcgccaa aggcgtgatc ggaatggccc agggcaagga catcaccggt
     6001 gaacgctccc aggccctgat gcgcaagcgc gtcctgtatc aggcgatcgc catcgtcctc
     6061 gtcgtcctgc tgctgctcgt cgccagcgga aagagctgag ccctggtcag gctcaaccag
     6121 atctacacgc gcaccggcga ttccggagcg tccggcctca ccgacggctc ccgccgctcc
     6181 aaggccgacc cgctcttcgc cgcgatcggc gacgtcgacg aagccaatag cgtgatcgga
     6241 gtcgccgtcc tcgaggtggc ggacgaggcc cggcggcgga tgcttaccgc catccagaac
     6301 gaattgttcg atctcggagc cgatctcgcc acgcccggcg aggatttcgc cccgtccgag
     6361 atgaccttgc gcatcgttca gccgcagatc gaccggctcg agctcgagat cgactcgatg
     6421 aacgaggggc tggagccgct gcgcagcttc atccttcccg gcggcggcgg cggagctgcg
     6481 gcgatccacc tcgcccgggc gatcgtccgc cgcgccgagc gctcggcgat cgccgcctcg
     6541 gaagcgaccc cgctcaaccc cctcgcactc atctatctga accgcctctc ggaccacctc
     6601 ttcgtcatgg cccgcgtggc ggcggcgggc gaaggcgggg aggtgctgtg gaagccgggg
     6661 gcgactcgat agcgcgtcac cccggcgaaa gccggggcct cggggagacc cgcggagact
     6721 tcttgtcctg aggtcccggc tttcgccggg atgacgggcg ctgcgactaa ccttcgtcaa
     6781 tattgtggta tagggaacgt tcgatggcga cccaggcaaa ttccccgccc gctttggccc
     6841 tggcccagcg ctaccgcgcc gtccgggcgc tgagcgaggc gatcgccgcc ccgctttccg
     6901 acgccgactc gactctccag ccgatgcccg acgcctctcc ggccaaatgg cacctagccc
     6961 acaccagctg gttcttcgag accttcgtcc ttcgcgacca cgtccccggc caccgcctct
     7021 acgatgagag ctgggcctat ctgttcaatt cctattacga gggcgagggc gagcgtcatc
     7081 cgcgcgaccg ccggggaatg ctcagccgcc cgagcctcga agaagtgcgc gcctggcggg
     7141 cctatgtcga cgaagcggtg gcggcggcgc tgccccggct tcccgaggac gcgctggccc
     7201 tggtcgagct cgggctcaac cacgagcagc agcatcagga gctgatgctg atggacctca
     7261 ccgccgcttt ggccgagact ccgctccgtc ccccgctctg ggccggcacc gcgccggcgc
     7321 ccgccgcgct cgcgccggcc atggagtgga tcgaggg
//
LOCUS       DASWNU010000032         6660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_182690, whole genome shotgun sequence.
ACCESSION   DASWNU010000032 DASWNU010000000
VERSION     DASWNU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182690"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VGB57_00670"
     CDS             complement(<1..1339)
                     /locus_tag="VGB57_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0129887.1"
                     /translation="MMADRHPGASRDLGEEGSDLPTGGPGFRRGDDSVANLPYLFAKR
                     NGIALVGEADGRVRVALRQGADPQALIEARRVLGRPLAVEAVTADGFDKLLSERYAMD
                     DRAAADAAGSLGLGDELSHLATDLPTADDLLDSADDAPAIRLINGIIADAARQGVSDI
                     HIEPYETGLVVRMRIDGVLRETLRMPAHVAPVVVSRIKVMARLDIAERRVPQDGRIGL
                     TLGGKLLDVRVSTLPSRAGERVVLRILDKENAGIDLDSLGMPPSVHALFAGALTEPNG
                     IVLVTGPTGSGTTTSLYAGLRLLNDGSRNVLTVEDPVEYAVDGVGQTQVNPKVGLSFA
                     AGLRAILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTVHTNDAIGAITRMRDMK
                     VEPFLLASTLRAVIAQRLVRRLCPACRAPVAADAATAALVGVEPGTIVHEPRGC"
     gene            complement(1336..3504)
                     /gene="gspD"
                     /locus_tag="VGB57_00675"
     CDS             complement(1336..3504)
                     /gene="gspD"
                     /locus_tag="VGB57_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY0129888.1"
                     /translation="MLAVPLTPLSAQHVVNFREADIRAFIEDAARVTGRTFIVDPAVS
                     GKVSVVTQRPLSRSEYFELFLSTLRANGLMAVPLRGGGFRIQPIATGAAAGGRVAARG
                     SANNFVTEIFRTRSIEGAQALEALRPLVSREGSITASRNSIVVADFADNVARIRQVLR
                     SIDRDTSTSRIVALRNAGAREIATSLNALMEGSSPNGRAATIVPVDSSNSIALRGDAA
                     TVARLVAMAEELDQRAATGTEIRVIFLQHADAAQLLPVLQQLLGQPVTQPPPTPGLSS
                     SRGEGATTSSTSRSGGGRPSVRTTTTTAEAPAPETAAATGVSIFGPRGAVVTRFQGAN
                     ALVIAASTDVQRQLGEVIRQLDTRREQVLVEAIIVEISDNAAKKLGVQLLLSGLGGSN
                     IPFAVTNYSNANPNLLTVAGAVAAERLRTTTTTVNGQVVTTTQNSPVADALQEAAVNS
                     VLGATGGIAGLAFRTGNAIFGAIINAVKSDSKSNILSTPSIMTLDNQEARILVGQEIP
                     ITTGEALSPNFDNQFRTVDRENVGITLEVLPQINAGGSIKLNLRQEVSSIAGPVSGRS
                     SDLILNKREIETVITVDDGEIVGIGGLLDDNERRTLEKIPFLGDLPAIGNLFKSKSRQ
                     RNKTNLMVFIRPTILRSAEDARAMTERRYGYIRGMQYLQNPDQEPTLDELVREYMGMV
                     PPDPAPAPPPPLDPAVYAPQTRPGDQVVAPPAAPPAGTPR"
     gene            complement(3531..4235)
                     /locus_tag="VGB57_00680"
     CDS             complement(3531..4235)
                     /locus_tag="VGB57_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY0129889.1"
                     /translation="MRLAFDSRRLIGRARGVNGWTLLELALLALIALQCARLIWTALT
                     PVGPLGDWQGTPAARSDASTGILGSFDPFFRLSGAAGPVTVTSLNLKLFGIRQDQASG
                     RGSAIIAGTDGQQRSIAVGEEIEPGVTLKSVDFDSVTISRGGADEQLFMDQSQAPNTV
                     TPTAPAAPTAPTPPVIQPSTSVEPGGPPVPPSRPQPTEVSAVPRMNGTQLASARVTLP
                     VERNGRVMTLRPGNGR"
     gene            complement(4384..5145)
                     /locus_tag="VGB57_00685"
     CDS             complement(4384..5145)
                     /locus_tag="VGB57_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0129890.1"
                     /translation="MTGLPAPADAAWTALAFILGAAIGSFLATILIRWPQGRSVVAGR
                     SACDKCGRTLEPRDLVPILSWSLARGRCRTCGTRIDRRHLAAEAGAAMVGLIAILAHP
                     MPLAAVTAILGWWLFLLAALDIEHEWLPDRLTLPLLGAGLAAAWGGFGPPLESRLIGA
                     AAGFASLFAIGALYRALRGREGLGGGDPKLFAAIGAWLGWTQLPFVLVGAGLLGLAAL
                     LLKRLRGGAVDATDRLPLGTLMALAAWPIWIVISL"
     gene            complement(5142..5990)
                     /locus_tag="VGB57_00690"
     CDS             complement(5142..5990)
                     /locus_tag="VGB57_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY0129891.1"
                     /translation="MRLRSLIFVPGDRPDRMEKALRAGADALILDLEDAVAPGAKPEA
                     RRHVAAFLNANSTAKLWVRINPLGSDDSDKDLAAILSSHPDGIVLPKAEGGVSVAELA
                     RRLTERGNATSRILAIATETPAAIFQLGSYGGAKRLAGLTWGAEDLPAAIGAATSREP
                     DGRFTPPYELARSLCLFGAAAAGVAPIETVYPAFRDLEGLAAYAARARRDGFTGMMAV
                     HPDQVPAINAAFTPSEAEVAHARAVVAAFEANPGAGALSLDGRMIDRPHLVQAQRTLA
                     AAAAGP"
     gene            complement(6100..6552)
                     /locus_tag="VGB57_00695"
     CDS             complement(6100..6552)
                     /locus_tag="VGB57_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0129892.1"
                     /translation="MAGRFFDEWTLGDRLAHQPHRTVTETDNLLISTLTHNPQPLHLD
                     AEHAATTEFGRIVVNGTFTFSLLVGLSVGDTTLGTLVANLGYDKVRMPKPVFIGDTLR
                     AETEVISLKDSKSRPDAGIVTFEHRMLNQRDELVCTTERTALIQRRGR"
BASE COUNT          982 a         2277 c         2399 g         1002 t
ORIGIN      
        1 cgcagccgcg cggctcgtgg acgatggttc cgggctcgac cccgaccagg gccgccgtcg
       61 cagcgtcggc ggcgaccggg gcccggcagg cggggcagag ccgccgcacc agccgctggg
      121 cgatcaccgc ccgaagcgtg gaggcgagca ggaacggctc caccttcatg tcgcgcatcc
      181 gggtgatggc gccgatcgcg tcgttggtgt ggaccgtgga gaggacgagg tggccggtca
      241 gcgaagcctg gacggctatc tcggcggtct cgcggtcgcg gatctcgccg accatgacga
      301 cgtcggggtc ctggcgcagg attgccctga gcccggccgc gaagctcagc ccgaccttcg
      361 gattgacctg ggtctggccc actccgtcga ccgcatattc gaccggatcc tcgacggtga
      421 ggacgttgcg gctgccgtcg ttgaggagcc tcaggccggc atagaggctg gtggtcgtgc
      481 ccgagccggt cgggccggtg acgaggacga tcccgttcgg ctcggtcagg gccccggcga
      541 agagcgcgtg gacggacggc ggcattccga gggaatcgag atcgattccg gcattctcct
      601 tgtcgaggat ccgaagcacg acccgctcgc ccgcccggct cggcagggtc gagacccgga
      661 cgtcgagcag cttgccgccc agggtcagcc cgatccgccc gtcctggggc accctccgct
      721 ccgcgatgtc gagccgggcc atgaccttga tccggctgac caccaccggg gcgacatggg
      781 cgggcatgcg caaggtctcg cgaagcacgc cgtcgatccg catccgcacc acgagccccg
      841 tttcataagg ctcgatgtgg atgtcggaga cgccttgccg ggcggcgtcg gcgatgatcc
      901 cgttgatcag ccggatggcg ggcgcgtcgt cggcgctgtc gagcaggtcg tcggcggtcg
      961 gcaggtcggt ggcgagatgg ctgagctcgt cgccgaggcc gagcgagccg gcggcgtcgg
     1021 ccgcggcccg gtcgtccatc gcgtagcgct cggagaggag cttgtcgaag ccgtcggccg
     1081 tgaccgcctc gaccgcgagc gggcggccga gcacccgcct cgcctcgatc agcgcctgcg
     1141 ggtcggcccc ctggcgaagc gccacccgga cccgcccgtc cgcttctccg acgagcgcga
     1201 tcccgttccg cttggcgaaa aggtagggca ggttggcgac actgtcgtca ccccggcgaa
     1261 agccggggcc tcccgtcgga aggtcgctgc cctcctcccc gaggtcccgg cttgcgccgg
     1321 gatgacggtc tgccatcatc gcggagtccc cgccggcggc gcggcggggg gcgccaccac
     1381 ctgatcgccc gggcgggtct gcggcgcata gacggccggg tcgagcggcg gcggcggggc
     1441 cggtgcgggg tcgggcggga ccatgcccat atattcgcgg acgagctcgt ccagggtcgg
     1501 ctcctgatcc ggattctgca gatattgcat gccgcggatg tagccgtagc ggcgctcggt
     1561 catcgcccgc gcatcctcgg ccgagcgcag gatggtgggc cggatgaaga ccatcaggtt
     1621 cgtcttgttg cgctgccggc tcttcgactt gaacagattg ccgatcgccg ggaggtcgcc
     1681 gaggaacggg atcttctcga gcgtccggcg ctcgttgtcg tcgaggaggc cgccgatccc
     1741 gacgatttcg ccgtcgtcga ccgtgatcac ggtctcgatc tcgcgcttgt tgaggatgag
     1801 gtcgctggag cggcccgaca cggggccggc gatcgagctc acctcctgcc tcaggttgag
     1861 cttgatcgag cctccggcat tgatctgcgg aagcacctcg agcgtgattc cgacattctc
     1921 gcggtcgacg gtgcggaact ggttgtcgaa attgggcgag agcgcctcgc cggtggtgat
     1981 cgggatctcc tggccgacca ggatccgggc ttcctggttg tccagcgtca tgatcgacgg
     2041 ggtggaaagg atgttcgact tgctgtcgga tttcaccgcg ttgatgatcg ccccgaagat
     2101 ggcgttgccg gttcggaagg cgaggccggc gatcccgccg gtggcgccga gcaccgaatt
     2161 gacggccgcc tcctggaggg catcggcgac cggcgaattc tgggtggtgg tcaccacctg
     2221 cccgttgacc gtggtcgtcg tcgtccggag ccgctccgcc gcgaccgctc ccgccacggt
     2281 caggaggttc gggttggcgt tggaataatt ggtgacggcg aaggggatgt tgctcccgcc
     2341 gaggcccgag agcagcagct ggactcccag cttcttggcg gcattgtccg aaatctcgac
     2401 gatgatcgcc tcgaccagca cctgctcgcg gcgcgtgtcg agctggcgga tgacctcgcc
     2461 caattgccgc tggacgtcgg tcgaggcggc gatgaccagg gcgttggcgc cctggaagcg
     2521 ggtcaccacg gcgccgcgcg gcccgaagat gctgactccg gtggccgccg cggtttccgg
     2581 ggccggggcc tcggcagtcg tcgtcgtcgt ccgcaccgac ggccgcccgc cgccgctgcg
     2641 ggaggtcgag gaggtcgtcg ccccctcgcc ccgcgacgag gacaggccgg gcgtcggcgg
     2701 cggctgggtc accggctggc cgagcaattg ctggagcacc ggcagcagct gcgcggcatc
     2761 ggcatgctgg aggaagatga ccctgatctc ggtgccggtg gcggcgcgct ggtcgagctc
     2821 ctcggccatg gcgacgagcc gggcgacggt ggcggcgtcg ccgcgaaggg cgatcgagtt
     2881 ggagctgtct acgggcacga tggtcgccgc ccgcccgttc gggctgctgc cctccatcag
     2941 ggcgttcagc gaggtcgcga tctcgcgggc gccggcattg cgcagggcga cgatccggct
     3001 cgtgctggta tcccggtcga tgcttcggag cacctggcgg atacgcgcca cgttatcggc
     3061 gaaatcggcc accacgatcg agttgcggct ggcggtgatc gagccttcgc ggcttaccag
     3121 cggacggagc gcctcgagcg cctgggcgcc ctcgatgctc cgggttcgga agatctcggt
     3181 cacgaaattg ttggcgctgc cccgcgcggc gacccttccg ccggcggcgg caccggtcgc
     3241 gatgggctgg atccggaagc ccccgccccg gagcggcacc gccatcaatc cgttggcgcg
     3301 cagggtcgag aggaacagct cgaaatattc cgagcgcgac agcggccgct gggtcaccac
     3361 cgaaaccttg ccgctcaccg ccgggtcgac gatgaaagtg cggcccgtca cccgggccgc
     3421 gtcctcgatg aaggcgcgga tgtccgcctc gcggaagttg acgacatgct gggccgagag
     3481 cggcgtcagc ggcaccgcca gcatcagggc gaaagcgagg aggccgcgtt tcaccggccg
     3541 ttccccggcc gcagggtcat cacccggccg ttacgctcca ccggaagcgt tacccgcgct
     3601 gaagccagct gggtcccgtt catccgcggc acggcgctga cctcggtcgg ctggggtctc
     3661 gacggcggca ccggcggccc gccgggctcg acggaggtgc tcggctggat caccgggggc
     3721 gtcggagcgg tcggagcggc gggggcggtc ggggtcaccg tgttcggcgc ctgcgactga
     3781 tccatgaaca attgctcgtc ggcgccgccc cgcgaaatgg tgacgctgtc gaaatccacc
     3841 gatttcagag tgactcccgg ctcgatctct tcgccgaccg cgatgctgcg ttgctggccg
     3901 tcggtgcccg cgatgatcgc ggagccgcgg ccggaagcct ggtcctggcg gatcccgaac
     3961 aatttgaggt tcagcgaggt gacggtcacc ggtccggcgg cgccgctgag acggaagaag
     4021 ggatcgaagc ttcccaggat cccggtcgag gcgtcgcttc gggccgccgg cgtgccctgc
     4081 cagtccccca gcggtccgac gggggtgaga gcggtccaga tcagccgcgc gcattgcagg
     4141 gcgatcaagg cgagcagagc cagctcgagc agagtccagc cgttgacgcc ccgggcgcgg
     4201 ccgatcagcc gccggctgtc aaaagctaaa cgcatctaaa ccagaacccc ttccccgcga
     4261 cacccaccgc ggtaatttcc acagtcgtaa caatgttttg gcgaccatcc agcccaggat
     4321 tcgccagccg gggaaacttc cagcttttcc gccgccgcgc aagcggcttg tgaggcaggc
     4381 cgattacagg ctgatgacta tccagatcgg ccaggcggcg agcgccatca gggtgccgag
     4441 cggcagccgg tcggtcgcgt ccaccgcccc gccccggagc cgcttgagga gcagagcggc
     4501 gaggccgagc aaccccgccc cgacgaggac gaacgggagc tgggtccagc ccagccaggc
     4561 gccgatcgcg gcgaacagct tggggtcccc gccgcccagc ccctcgcggc cgcgcagcgc
     4621 ccggtagagc gctccgatcg cgaacagcga agcgaagccg gcggcggcgc cgatcagccg
     4681 gctttcgagc ggcgggccga acccgcccca cgccgccgca agtccggctc caagcagcgg
     4741 caaggtcagc cggtcgggaa gccattcatg ttcgatatcg agcgccgcga gcaggaacag
     4801 ccaccagccc aggatcgccg tcaccgccgc cagcggcatg ggatgggcga ggatggcgat
     4861 gagcccgacc atggccgcgc ccgcctcggc cgccagatgc cgccggtcga tgcgcgtgcc
     4921 gcaggtccgg caccggccgc gcgccagcga ccagctgagg atcggcacca gatcgcgcgg
     4981 ctccagggtg cgaccgcatt tgtcgcaggc ggagcggccg gcgacgacgc ttcgcccctg
     5041 cggccagcgg atcaggatcg tcgcgaggaa gctgccgatg gcggcgccga ggatgaaggc
     5101 gagcgcagtc caggcggcgt ccgcgggcgc cggcaatccg gtcacggacc ggcggccgcc
     5161 gcggcgagcg tgcgctgtgc ctggacgaga tgcggccggt cgatcatccg cccgtcgagc
     5221 gacagcgccc ccgcccccgg attcgcctcg aacgcggcaa cgaccgctcg ggcgtgcgcg
     5281 acctcggcct cgctcggagt gaaggcggcg ttgatcgccg gcacctggtc cgggtggacg
     5341 gccatcatcc cggtgaagcc gtcccgccgc gcccgcgccg cataggccgc gagcccctcg
     5401 aggtcccgga atgccggata gacggtctcg atcggagcca cgcctgccgc cgcggcaccg
     5461 aacaggcaga gcgagcgggc gagctcgtat ggcggggtga agcggccgtc cggctcgcgc
     5521 gacgtcgccg ccccgatcgc cgccggcaaa tcctccgcgc cccaggtcag tcccgccagc
     5581 cgcttggctc cgccatagct gccgagctgg aagatcgccg ccggggtctc ggtcgcaatg
     5641 gcgaggatcc gcgaagtcgc gttgccgcgc tcggtcagcc gccgggccag ctcggccacc
     5701 gacacgccgc cctccgcctt gggcaggacg atcccgtccg gatggctgga gaggatggcg
     5761 gcgaggtcct tgtcgctgtc gtcgctgccg agcgggttga tccgcaccca gagcttggcc
     5821 gtgctgttgg cgttgaggaa cgccgccacg tggcgcctgg cctccggctt cgcgccaggc
     5881 gcgacggcgt cctccagatc gaggatcagg gcgtcggcgc cggcgcgaag cgccttttcc
     5941 atccggtccg ggcgatcgcc ggggacgaaa atgagggaac gaagcctcat cgaatatcgt
     6001 catgccggac ttgatccggc atccaccttg tctctgtccg cgcaatacga ggagaagctg
     6061 gaccccggat caagtccggg gtgacgattt ccacgatcct caccgccccc tccgctggat
     6121 cagtgccgtg cgctccgtcg tgcagacgag ctcgtcgcgc tggttgagca ttcggtgctc
     6181 gaacgtcacg atcccggcat cgggccgcga cttcgaatcc ttcagcgaga tcacttcggt
     6241 ctcggcgcgc agcgtgtcgc cgatgaacac cggcttgggc atccgtacct tgtcgtagcc
     6301 gaggttcgcg acgagggttc cgagcgtcgt atcgccaacc gacagcccga ccagcaaaga
     6361 gaaggtgaag gtgccgttga cgacgatccg gccgaactcg gtggtcgccg catgttcggc
     6421 gtcgaggtgc agcggctgcg gattgtgggt gagcgtggaa atcaggagat tgtcggtctc
     6481 cgtgacggtg cgatgcggct gatgcgccag acgatcgccc agcgtccact catcaaagaa
     6541 gcgcccggcc acgggggcta cacccccgcc ccggcgacgg ccggggtctc gtgacaagtg
     6601 gtcaccgggg gtctccacgc gatccccacc cttttcgtca tcccggcgag ggccgggatc
//
LOCUS       DASWNU010000033         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_52920, whole genome shotgun sequence.
ACCESSION   DASWNU010000033 DASWNU010000000
VERSION     DASWNU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52920"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGB57_00700"
     CDS             complement(<1..543)
                     /locus_tag="VGB57_00700"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY0129893.1"
                     /translation="MAEAGPGAPGGYVAFISYSHKDAAMGRWLHRKLEGYRLPKRLAG
                     TQGEDGEVPERLTPIFRDRDELPAAGDLSERVRAALAVSRNLIVLCSPNSAASPWVAK
                     EIATFRELHPGRPIFTAIVEGEPGQCFSPALLEGGTEPLAADLRKEGDGRRLGLLKLV
                     AGLAGVGLDAVVQRDAARRVR"
     gene            complement(682..2199)
                     /locus_tag="VGB57_00705"
     CDS             complement(682..2199)
                     /locus_tag="VGB57_00705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0129894.1"
                     /translation="MSFADLGLSDELLRAVEESGYTDPTPIQTQAVPSVLMGRDLIGI
                     AQTGTGKTAAFVLPMIDILGEGRSRARMPRSLILEPTRELAMQVSENFEKYGKYHKLN
                     MALLIGGTNMADQIKKLEKGVDVLIATPGRLMDLFGRGKIMLNGCGLLVIDEADRMLD
                     MGFIPDIEEICSKLPAVRQTLLFSATMPGPIKKLADKFLTGPKTIEVARPATANISID
                     QRVLKVDSRKKKQVLTDRLKGNDVRTAIVFCNRKTTVRELMQSLKRSGLNAGEIHGDM
                     EQPERIRELDRFKSGEINILVASDVAARGLDIKDVSHVFNYDVPWHPDDYIHRIGRTG
                     RAGKTGIAITLATAADAEAIEAIEKLTGAKIRPVEGAADPAESAPPEGEEEERPRRER
                     GARPPRGRGRSEAPKREPRRRAEPEAASGTEPEAAPEPKPAAPRAEPRRERAEAEPRA
                     RRDEPRREPRPARGHAEPRPEPARGRAESRPEPPAAEGEEGWNGPVPSFLAHKLG"
     gene            complement(2285..2716)
                     /locus_tag="VGB57_00710"
     CDS             complement(2285..2716)
                     /locus_tag="VGB57_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129895.1"
                     /translation="MIRAGAAALLLLLAAAAPAAAPPARPAQYGQRVVREQVIVRFRQ
                     TPPSRLGWKEGKGPKCVAARAIAGATLVGKSSVDFILRSRQRVRAKLESSCPALDYYY
                     GFYITPNADGMICEDRDNIRSRVGGACEIDNFRSLEPVAPR"
     gene            2883..>3539
                     /locus_tag="VGB57_00715"
     CDS             2883..>3539
                     /locus_tag="VGB57_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEY0129896.1"
                     /translation="MATEPPSTGLPIFYGGLEPLSSSVHGGYRSRTSDRAPFLTSNHA
                     VPITVDEFVAIQRHFPIVFSIGDNPVPLALMGLNEGVNVFVDETGKPIGDIYLPAYVR
                     RYPFMLARLQPDAQELSLCFDPTSGLVGDFEEGDALFDDGKPSATTNNILKFCEEFEL
                     SAQRTVAFMTELKDAGLLMDGEVSIQPMGSEQPFVYRGFQMINEEKLRLLEGDDLKRM
                     N"
BASE COUNT          536 a         1197 c         1195 g          611 t
ORIGIN      
        1 gcgcactcgc cttgcggcgt cgcgctggac cacggcgtcc aggcccaccc cggccaagcc
       61 ggcgaccagc ttaagcaggc ccagcctccg gccatcccct tccttgcgca ggtcggcagc
      121 cagcggctcc gtgccacctt ccagcagggc cggcgagaag cattggcccg gctccccctc
      181 cacaatggcg gtgaagattg gccgtcctgg atgaagctct cggaaggtcg cgatctcctt
      241 ggcgacccag ggagaggcgg cgctgttggg ggagcagaga acgatcaggt ttctcgatac
      301 ggccagcgcc gcccgaaccc tctcgctgag gtcgccggcc gccggaagtt cgtcgcggtc
      361 gcggaagatg ggggtcagcc gctccggtac ctcgccatct tcgccctgcg tccccgccaa
      421 ccgcttcggc agccgatacc cctccagctt gcgatgaagc caccgcccca tcgccgcgtc
      481 cttgtggctg tagctgatga acgcgacata gcccccgggt gcgccgggtc ccgcttcggc
      541 catggatagt cccccatggg cgcgggaggc tcggccgcgc ccgccggcaa ggtgccagag
      601 ggcggacgag tccacaacat atcaaaaact tagattcgga cggcgtaaat cgggaagtcg
      661 gataacgtcg tcccgagccg atcagcccag cttgtgggcc aggaagctcg gcaccgggcc
      721 gttccagcct tcttcgccct cggccgctgg cggctcgggc cgggactcgg cacggccgcg
      781 ggccggctcg ggccggggct cggcatggcc gcgggcggga cgaggctcgc gtctcggctc
      841 gtcgcggcgg gcgcgaggct ccgcttcggc ccgttcccgg cgaggctccg cccgcggcgc
      901 ggcgggcttc ggctcgggcg cagcttcggg ctccgtcccg gacgcggctt caggctcggc
      961 ccggcgcctc ggctcccgct tcggcgcctc cgatcggccc cgtccgcgcg gcggccgggc
     1021 gccgcgttcg cggcggggac gctcctcctc ctcgccctcg ggaggcgcgg actcggccgg
     1081 atccgccgcg ccctcgaccg gacggatctt agctccggtc aatttctcga tcgcctcgat
     1141 cgcctcggcg tcggcggcgg tggcgagggt gatggcgatt ccggtcttgc ccgcccggcc
     1201 ggtgcggccg atgcggtgga tgtaatcgtc cgggtgccac ggcacgtcgt agttgaagac
     1261 gtggctgacg tccttgatgt cgaggccccg ggcggcgacg tcggaggcga cgaggatgtt
     1321 gatctcgccg gacttgaagc ggtcgagctc gcggatgcgc tcgggctgtt ccatgtcgcc
     1381 gtggatctcg ccggcgttga ggcccgagcg cttgaggctc tgcatgagct cgcgcaccgt
     1441 cgtcttgcgg ttgcagaaga cgatggcggt gcgcacgtca ttgcccttga ggcggtcggt
     1501 caggacctgc ttcttcttgc gcgaatcgac cttcaggacg cgctggtcga tcgagatgtt
     1561 ggcggtggcg ggccgggcga cctcgatcgt cttggggccg gtcaggaact tgtcggccag
     1621 cttcttgatc ggccccggca tggtcgccga gaaaagcagg gtctggcgca ccgctgggag
     1681 cttcgagcag atctcctcga tgtcggggat gaagcccatg tcgagcatcc ggtccgcctc
     1741 gtcgatgacg agcagaccgc agccgttgag catgatcttg ccgcgcccga acaggtccat
     1801 cagccggccc ggcgtggcga tcaggacgtc gacgcccttc tcgagcttct tgatctggtc
     1861 ggccatgttg gtgccgccga tcagcaaggc catgttgagc ttgtgatact tgccgtattt
     1921 ctcgaaattc tcggaaacct gcatcgccag ctcgcgggtc ggctcgagga tcagcgagcg
     1981 gggcatgcgc gcgcggcttc ggccttcgcc gaggatgtcg atcatcggga ggacgaaggc
     2041 ggcggtcttg ccggtccccg tctgggcgat tccgatcagg tcgcggccca tcaggacgga
     2101 ggggaccgcc tgggtctgga tcggggtggg atcggtgtag ccggattcct ccacggcgcg
     2161 gagcaattcg tcggaaaggc cgaggtcggc aaaactcata agggttccgg gtggttgagg
     2221 ggcttcgtcg agtgccgctt caggtcagcc gcttgggcga agcggcggcc actgtcaaga
     2281 aagctcaacg gggcgcgacc ggttcgaggc ttcgaaaatt gtcgatctcg caagctccgc
     2341 ccacccggga gcggatgttg tcgcgatcct cgcagatcat gccgtcggca tttggggtga
     2401 tgtagaagcc gtaataataa tcgagcgccg ggcagctgct ctcgagcttc gcgcgcaccc
     2461 gctgacggct gcgcaggatg aagtcgacgc tgctcttgcc gaccagggtg gccccggcga
     2521 tggcgcgcgc cgcgacgcac ttcgggccct tgccctcctt ccagcccagt ctcgaagggg
     2581 gagtctggcg gaagcggacg atgacctgct cgcgcacgac cctctgcccg tattgcgccg
     2641 gccgagcggg cggcgcggcc gccggagcgg cggccgcgag caggagcagc agggcggcgg
     2701 cgcccgcgcg gatcatgatg aggcctccat ggcctcgtcc cttccccgca aacgcttgaa
     2761 catgcgatta atcccccgcg ccgcatccgc gcttgcgaaa gcccggattg cgccatacta
     2821 ccccgcccca ttcaggcggc ccggaacgcg gccgccccca acagctagcg gagacaacga
     2881 acatggcgac cgagccgccg tcaaccggcc tgccgatttt ctatggcggc ctcgagccgc
     2941 tgtcgagcag cgtccatgga ggctatcgca gccgcacttc ggatcgcgcg cccttcctca
     3001 ccagcaacca cgcggtgccg atcacggtcg acgagttcgt cgccatccag cgccatttcc
     3061 cgatcgtctt ctcgatcggc gacaatccgg tgccactggc gctgatgggc ctcaatgaag
     3121 gcgtcaacgt cttcgtcgac gagaccggca agccgatcgg cgacatctat ttgcccgctt
     3181 acgtccgccg ctatccgttc atgctcgccc gccttcagcc ggacgcccag gagctttcgc
     3241 tctgcttcga tccgaccagc ggcctggtcg gcgatttcga ggaaggcgat gcccttttcg
     3301 acgacggcaa gccgagcgcg acgacgaaca acatcctcaa attctgcgag gaattcgagc
     3361 tttcggccca gcgcaccgtc gccttcatga ccgaattgaa ggacgccggc ctgctgatgg
     3421 acggcgaagt ctcgatccag ccgatgggct ccgagcagcc cttcgtctat cgcggcttcc
     3481 agatgatcaa cgaggagaaa ttgcgactcc tcgaaggcga cgatctgaaa cggatgaac
//
LOCUS       DASWNU010000034         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_364418, whole genome shotgun sequence.
ACCESSION   DASWNU010000034 DASWNU010000000
VERSION     DASWNU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364418"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VGB57_00720"
     CDS             <1..846
                     /locus_tag="VGB57_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129897.1"
                     /translation="YVHDFGSGQTRCASCPSTGSDTVANASFNPPIARFSVSANTRST
                     VPRGFAADGRLYFQAEDALVPEDVNGVEDVYAYSFDGDDVELISSGTGGGSSISDISE
                     DGSSVFFLTEESLIPQDIDGGAPDIYVSRVGGGFLAPPPVDAGSCRDDTCQGVPSAGA
                     AKPSIGSVRFGRGTAVRLKVQGAKRVFAKPTALLRAAVPGPGRITVTGAAVIKVSRAA
                     SGAKPVSLPVRLDRAARKRFAAGDRVRAAVVVSFAAADGGSTARSLTLTFVRAAGSNA
                     KGGRR"
     gene            843..>3414
                     /locus_tag="VGB57_00725"
     CDS             843..>3414
                     /locus_tag="VGB57_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129898.1"
                     /translation="MSRFARTLVAAVLIGCAALSIAAPAQAVFGIESFDMRSVDPLGR
                     SLTQAGAHADVETDFRLETITDSYGVIPDETLKEMAVNLPPGLFGMPRNVPECTVAEF
                     TNMFAGGCPEATQVGVSYVELPVAGSTTETHEQRSPVYNLAPQRGELAVLGFHVTFVP
                     VRVVARLRTEGDYGIEMRATNLSRAANVFGQRITIWGVPADPAHNPERGIGGGETTNQ
                     PRTPFLALPATCSAPSLATAQGRSWESPDLWDEAVDSMERQIGCERQQFQPSISVRPT
                     STAPDGPTGLDVEIRSPQNQDPKGIGTPPFKDVVVTFPEGMSVNPSVADGLVACPDEQ
                     LNLRSRAPFSCPPASRLGTVEAESPALEEKLVGGLYARPQLSDDPASGEMFRIAMVLE
                     SPERGLSVRLPGQVRVDPAGGRVQTIFADNPQLPVEWIRLRLKDGPRSPLANPPRCGT
                     YATEAKISSWGGQEVELRDQFEISCATRAFTPGFEAGTVDPRAGGFSPLVVRIDRADG
                     EQYLAGVSVDMPGGLLAKLAGVGLCPEAVAGDGTPGSCPAASRVGSVTTSAGAGSPFS
                     LRGGVYLTGPYKGAPYGLSVQVPAQAGPFNLGIVKVRQALHVNPITASVRAVSDPLPT
                     IVKGVPVRIRGVQVEIDRSQFTFNPTGCESESIGARLTSAAGAIFNTSSPFRAVDCKD
                     LGFEPKLSFRLKGETHRSAHPAVTATLKARKGDANIGKAVVTLPKTQFLAQEHIRTIC
                     TRVQWAAETCPKAAIYGYAKAWTPLLDKPLEGPVYLRSSSNELPDLVADLNGQIEIDL
                     AGRIDSVRQRMRTTFWAVPDAPVSKFVLTMQGGRKGLLVNNTELCRAKPRARAEFTGH
                     N"
BASE COUNT          510 a         1287 c         1127 g          490 t
ORIGIN      
        1 tacgtgcacg acttcgggtc gggccagacg cgctgcgcct cctgcccgtc caccggctcc
       61 gacacggtcg ccaacgcttc gttcaacccg ccgatcgccc gcttctcggt ctccgcgaac
      121 acccgctcga ccgttccgcg cggattcgcc gccgacggcc ggctctactt ccaggccgag
      181 gacgcccttg tccccgagga cgtgaacggc gtcgaagacg tctacgccta ctccttcgac
      241 ggcgacgacg tcgagttgat cagcagcggc accggcggcg gcagctcgat ctcggacatc
      301 agcgaggacg gcagcagcgt cttcttcctc accgaggaga gcctgattcc gcaggacatc
      361 gacggcggcg cgccggacat ctacgtctcc cgggtcggcg gcggcttcct cgccccgccc
      421 ccggtcgacg ccggcagctg ccgcgacgac acctgccagg gcgtgccgag cgccggcgcg
      481 gccaagccga gcatcggctc ggtgcggttc ggccgcggga ccgcggtgcg gctgaaggtg
      541 cagggggcca agcgggtctt cgcgaagccg acggcgctgc tgcgggccgc ggttcccggc
      601 cccggccgca tcaccgtcac cggcgccgcc gtcatcaagg tgagccgcgc cgcctccgga
      661 gcgaagccgg tctccctgcc ggtccgcctc gaccgcgccg cccgcaagcg gttcgccgcc
      721 ggtgaccggg tgcgcgccgc ggtggtcgtc tccttcgccg cggccgatgg cggctccacc
      781 gccagatccc tgaccctgac cttcgttcga gccgccggct cgaacgcgaa aggaggccgg
      841 cgatgagccg cttcgcacgc accctggtcg ccgccgtcct gatcggctgc gcggccctct
      901 cgatcgccgc cccggcccag gccgtcttcg gcattgaatc gttcgacatg cggagcgtcg
      961 acccgctcgg ccggtcgctg acgcaggccg gcgcgcacgc cgacgtcgaa accgacttcc
     1021 ggctggagac gatcaccgac agctacggcg tcatccccga cgagacgctg aaagagatgg
     1081 cggtcaacct gccgccgggg ctcttcggca tgccccgcaa cgttcccgag tgcacggtcg
     1141 ccgaattcac caacatgttc gccggcggct gtccggaggc cacccaggtc ggcgtctcct
     1201 acgtcgagct gccggtcgcc ggcagcacca ccgagaccca cgaacagcgc agccccgtct
     1261 acaacctggc gccgcagaga ggcgagctgg cggtcctcgg cttccacgtc accttcgttc
     1321 cggtgcgggt cgtggcccgg ctgcggaccg agggcgacta cgggatcgag atgcgggcca
     1381 ccaacctcag ccgggcggcc aacgtgttcg gccagcggat caccatctgg ggcgtgcccg
     1441 ccgatccggc gcacaacccc gagcggggca tcggaggcgg cgagaccacg aaccagccgc
     1501 gcacgccctt ccttgccctg cccgcgacct gctcggcccc gtccctcgcc acggcgcaag
     1561 ggcgctcgtg ggagagcccg gatctctggg acgaagccgt cgactcgatg gagcggcaga
     1621 tcggttgcga gcgacagcag ttccagccct cgatctcggt taggcccacc tccacggcgc
     1681 ccgacgggcc gaccgggctc gacgtcgaga tccgctcgcc tcagaaccag gatccgaaag
     1741 ggatcggaac gcctcccttc aaggacgtcg tcgtcacctt cccggagggg atgtcggtca
     1801 acccctcggt ggccgacggc ctcgtcgcct gccccgacga gcagctgaac ctgcgctcgc
     1861 gggcgccctt cagttgcccg ccggcctcca ggctggggac cgtcgaagcg gaaagcccgg
     1921 cgctcgagga gaagctggtg ggcggtctct acgcgcgtcc ccagctctcc gacgatcccg
     1981 cctcgggcga gatgttccgc atcgcgatgg tgctcgagag tcccgagcgc ggcctctcgg
     2041 tgaggctgcc cggccaggtc cgggtcgatc ccgccggcgg tcgggtgcag acgatcttcg
     2101 ctgacaatcc gcagctgccg gtcgagtgga tccggctgcg gctcaaggac ggcccgcgct
     2161 cgccgctcgc caacccgccg cgctgcggca cctacgcgac cgaggcgaag atctcctcct
     2221 ggggcggtca ggaggtcgag ctgcgcgacc agttcgagat cagctgcgcc acgcgcgcct
     2281 tcacccccgg cttcgaagcc gggacggtcg atccccgcgc cgggggcttc tctccgctgg
     2341 tggtgaggat cgacagggcc gacggagagc agtacctggc cggtgtcagc gtcgacatgc
     2401 ccggcgggct gctcgcgaag ctggccggcg tcggcctctg cccggaggcg gtggccggcg
     2461 acggaacccc cggttcctgc cccgcggcgt cgcgggtcgg ttccgtcacc acgagcgccg
     2521 gggcgggcag ccccttctcg ctccggggcg gggtctatct gaccggtccc tacaaaggcg
     2581 ctccctacgg cctttcggtg caggtgccgg cgcaggccgg gcccttcaac cttggaatcg
     2641 tcaaggtgcg ccaggcgctg cacgtcaatc cgatcacggc ctcggtgcgc gcggtctccg
     2701 atcccctacc gacgatcgtc aagggcgtgc ccgtccgcat ccgcggcgtc caggtcgaga
     2761 tcgatcgctc ccagttcacc ttcaacccca ccggctgcga gtcggagagc atcggcgcca
     2821 ggctgacctc ggcggcgggc gcgatattca acacctcctc gccgttcaga gcggtcgact
     2881 gcaaggacct cggcttcgaa ccgaagctct cgttccgcct caagggcgag acccatcgca
     2941 gcgcccaccc ggccgtcacc gcgaccctga aagcccgcaa aggcgacgcc aacatcggca
     3001 aggcggtcgt caccctgccg aagacccagt tcctcgccca ggagcacatc cgcacgatct
     3061 gcacccgcgt gcagtgggcc gcggagacct gtccgaaagc ggcgatctac ggctacgcga
     3121 aagcctggac gccgttgctg gacaagcctc tcgagggccc ggtctatcta cggagctcca
     3181 gcaacgaact ccctgacctg gtcgccgacc tcaacggcca gatcgagatc gacctcgccg
     3241 gtcgcatcga ctcggtcagg cagaggatgc ggaccacctt ctgggcggtt cccgacgccc
     3301 cggtctccaa attcgtgctc accatgcagg gcggcaggaa gggcctgctc gtaaacaaca
     3361 ccgagctttg ccgggcgaag ccgcgggcga gagccgagtt caccggccac aacg
//
LOCUS       DASWNU010000035         6418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_208813, whole genome shotgun sequence.
ACCESSION   DASWNU010000035 DASWNU010000000
VERSION     DASWNU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208813"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1548)
                     /locus_tag="VGB57_00730"
     CDS             complement(52..1548)
                     /locus_tag="VGB57_00730"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0129899.1"
                     /translation="MRNIDHFIGGGAHASGERHGDVFDPNNGGVQAKVRLGTAADLER
                     AVAAAREAFPAWAATNPQRRARVMFNFKALVEENMEDLALLLSSEHGKVIADAKGDIQ
                     RGLEVIEFACGIPHALKGEYTQGAGPGIDVYSMRQPLGLVCGITPFNFPAMIPMWMFG
                     VAIACGNAFILKPSERDPSVPVRLAELMKAAGLPDGILQVVHGDKEMVDAILDHDDVK
                     AISFVGSSDIAQYIYSRGTANGKRVQAFGGAKNHGIVMPDADLDMVVNDLAGAAFGSA
                     GERCMALPVVVPVGEKTAENLREKLIPAIEGLRVGVSTDSEAHYGPVVNAAHRERIEN
                     YIQMCADEGGELVVDGRGFRLQGHEEGFFIGPTFFDHVKPSFRSYQEEIFGPVLQMFR
                     ADSFEEAVRLPSDHQYGNGVAIFTRNGHAAREFAARVNVGMVGINVPIPVPVAYHTFG
                     GWKRSGFGDINQHGMEGVRFWTKTKTVTQRWPDGSVTGENAFVIPTMG"
     gene            1712..2719
                     /locus_tag="VGB57_00735"
     CDS             1712..2719
                     /locus_tag="VGB57_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0129900.1"
                     /translation="MKLGSLKGGRDGRLVVVSNDLAWCADATHIAPTLQAALDDWDRL
                     APQLELLAMDLEHVVIPRDRFHEREALAPLPRAYQWADGSAYVNHVALVRQARAAEMP
                     ESFWTDPLMYQGASDEMLAPRSPIPLADEAWGCDCEAEVVVVTDDVPQGITAEDAGAH
                     ILLVGLVNDVSLRNLIPGELGKGFGFLQSKPASALSPVFVTPEALGDRFRDGKLHGAL
                     KVDLNGKPFGRADAGVDMTFDFGTLIAHLAKTRNIGAGSIIGSGTVSNRDSDGGPGKP
                     IEAGGLGYSCIAEVRTVETILEGKPRTEFLKRGDTVRIWMDDEAGHPIFGVIEQTVAQ
                     G"
     gene            2894..3811
                     /locus_tag="VGB57_00740"
     CDS             2894..3811
                     /locus_tag="VGB57_00740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0129901.1"
                     /translation="MERRKIRERVRELAAQGGEWIYDIPLPHGVWTGGKLNVPHTRLK
                     RIVQIIGDLMDKPLSRCRVLDLGSLEGLFSIEMAQQGAETVGVEIRRQNLDKAEFARK
                     LLGLTNLQFRQDDVRNISVAGYGRFDAIICSGILYHLPAGDVIDLIRTMYAMADRAVV
                     IDTHVALAAERAFTRGGETYRGRDHSEFGPGMSDAQKAASRLSSADNDTSFWLTRPSL
                     INAMSAAGFSSVYECFTPAHLNFGRPGFESRDRVTFVGIKGEKVRLNNSPAANDLDER
                     WPEGALSYAPPSNSLGRRGKNLVKRLLGR"
     gene            complement(3981..>6418)
                     /locus_tag="VGB57_00745"
     CDS             complement(3981..>6418)
                     /locus_tag="VGB57_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0129902.1"
                     /translation="RERDRRMHEIIDATPLPNWAYVVPKTLAVTLVLFATLLFGMVAA
                     ILVQLGKGYTDIELGKYLLWYLLPLGVEVTMIAVLAVFVQSISPNKYVGWAVMVVYIV
                     ATITLVSMGFEHNLYQYNGSPNVPLSDMNGGGGFAVGAWWFRLYWAAFATLLLVASHL
                     LWRRGTETRLKPRLRRAPRRLAGAPGIIAGLALTVFVATGAYAWYNTNVINTFETSSD
                     RDKFSAEYEKKYLKNEALPHPAISKVVLNVALYPSETRAVIDGRYVISNLTSAPIRDV
                     HVRMPPNDLELLRLDFPGARLRLDDKEYGYRIYALDTPMQPGETRALSFQTRRWSRGF
                     RNSGHDIKLVRNGTFLNNMEILPAIGMDRGGMLQDRSKRRKYGLPPIQRAAKLEDLSA
                     TRKSYFGGGWSTADITVSTEADQIPIAPGKKVSDVVGGGRRTARFVSDAPILTFFSIQ
                     SARYAEKHRNHRGIDLAVYYHPTHDRNVDRMLDALAGAMDYYQTAFGPYQFDQARIIE
                     FPAYSRFAQAVANTMPYSEAIGFVADTSNPEKIDYVTYITAHELGHQYWGHQIIGAEM
                     QGGTMLSETLAQYSALMVMKQLYGDDKIRRFLKYELDSYLRLRGGELVEELPLMRVEN
                     QQYIHYNKGSLVMYLLQDRLGEAAVNRAIRSVLEKYKFKGAPYPRSIELVEAFRREAK
                     SPEDQALITDLFERITVYDLKVGEAKATKRADGKWDVVVPVEAKKAYADGQGNEKPAA
                     FADRIAIGLFTAAPGSGAFDRSNVLTMQRQPVKSGRQVFRFVTDRKPTHAGIDPYNFY
                     IDRNSDDNVGAVI"
BASE COUNT         1096 a         2049 c         2139 g         1134 t
ORIGIN      
        1 ttgcgttttc gttcaaatga tcaaaatact cactggtttc gtaagaacga atcaccccat
       61 ggtcgggatc acgaacgcat tctcccccgt cacgcttccg tcgggccagc gctgggtgac
      121 ggtcttggtc ttggtccaga acctcacccc ttccatgccg tgctggttga tgtccccgaa
      181 gcccgaccgc ttccagccgc cgaacgtgtg gtaggcgacc gggaccggga tcggtacgtt
      241 gatcccgacc atcccgacat tgacccgggc ggcgaactcg cgcgccgcat ggccgttgcg
      301 ggtgaagatg gcgactccgt tgccatattg gtggtcggag gggaggcgga cggcctcctc
      361 gaagctgtcg gcccggaaca tctggaggac ggggccgaaa atctcctcct gataggagcg
      421 gaagctcggc ttcacatggt cgaagaaggt cgggccgatg aagaagccct cctcatggcc
      481 ctgaagcctg aagccgcggc cgtcgacgac cagctcgccg ccctcgtcgg cgcacatctg
      541 gatgtaattc tcgatccgct cgcgatgggc ggcgttgacc accggaccgt aatgggcctc
      601 ggagtcggtc gagacgccca ccctcagccc ctcgatcgcg gggatcagct tctcgcgcag
      661 gttctcggcg gtcttctcgc ccaccggaac caccaccggc agcgccatgc agcgctcgcc
      721 cgccgagccg aaggcggctc cggcgaggtc gttcacgacc atgtccagat cggcgtcggg
      781 catgacgatg ccgtggttct tggcgccgcc gaaggcctgg acccttttcc cgttcgcggt
      841 gccgcggctg tagatatact gggcgatgtc gctgcttccg acgaagctga tcgccttcac
      901 gtcgtcatgg tcgaggatgg cgtcgaccat ctccttgtcg ccgtggacga cctggaggat
      961 cccgtcggga agccccgccg ccttcatcag ctcggcgagc cggaccggca cggaggggtc
     1021 ccgctccgag ggcttgagga tgaaggcgtt gccgcaggcg atggcgaccc cgaacatcca
     1081 catcgggatc atcgcgggga agttgaacgg ggtgatcccg cagacgaggc cgagcggctg
     1141 gcgcatcgaa tagacgtcga tgccgggccc ggcgccctgg gtatattcgc ccttcagggc
     1201 atgcgggatg ccgcaggcga actcgatcac ctcgagcccg cgctggatgt cgcctttggc
     1261 gtcggcgatc accttgccgt gctcggacga gaggaggagc gcgaggtcct ccatattctc
     1321 ctcgaccagg gccttgaagt tgaacatcac ccgggcccgg cgctgggggt tggtcgcggc
     1381 ccaggcgggg aaggcctcgc gggcggcggc gaccgcgcgc tcgaggtcgg ccgcggtgcc
     1441 gaggcggacc ttcgcctgca ctccgccatt gttggggtcg aacacgtcgc cgtgccgctc
     1501 gccggaggcg tgggcgccgc cgccgatgaa atgatcgatg ttacgcatga ttcagtcctt
     1561 ggacgggaaa tttggggttg gcagcccact agccaaggcg cggcgcaaat gccagtgcac
     1621 cccgtcatcc ggcgaaagcc gggacctcat ggtgaagtgc cgtgacctct cgtcctgggg
     1681 tcccggcatt cgccgggatg acggaggaaa gatgaaactg ggttccttga agggcggccg
     1741 cgacgggcgg ctggtggtgg tcagcaacga tctcgcctgg tgcgccgacg cgacccatat
     1801 cgccccgact ctgcaggcgg cgctggacga ttgggaccgg ctcgccccgc agctggaact
     1861 gctcgccatg gatctggagc atgtcgtcat cccccgcgac cgcttccacg agcgcgaggc
     1921 gctggcgccc ttgccgcgcg cctatcagtg ggcggacggc tccgcctatg tgaaccacgt
     1981 cgccctcgtc cgccaggccc gcgccgccga gatgccggaa agcttctgga ccgatccgct
     2041 catgtaccag ggcgcctccg acgagatgct tgcgccgcgc tccccgatcc cgctcgccga
     2101 cgaagcctgg ggctgcgact gcgaggcgga agtggtggtg gtcaccgacg acgtccccca
     2161 gggcatcacg gccgaggacg cgggggccca catcctgctc gtcggcctcg tcaacgacgt
     2221 ctccctgcgc aacctcatcc ccggcgagct cggcaagggc ttcggcttcc tccagtccaa
     2281 gcccgcctcg gctctgtcgc cggtcttcgt cacccccgag gcgctcggcg accgcttcag
     2341 ggacggcaag ctccacggcg cgctcaaggt cgatctgaac ggcaagccgt tcggccgggc
     2401 cgacgccggc gtcgacatga cgttcgattt cggaaccctg atcgcccacc tggcgaagac
     2461 ccggaacatc ggcgccggct cgatcatcgg ctcgggcacc gtctccaacc gcgattcgga
     2521 cggcggcccc ggcaagccga tcgaggcggg cggcctcggc tattcgtgca tcgccgaggt
     2581 gcggaccgtc gagaccatcc tggagggcaa gcccaggacc gagttcctga agcgcggcga
     2641 cacggtacgg atctggatgg acgacgaagc cggccacccg atcttcgggg tgatcgagca
     2701 gacggtggcg cagggataac tcaggctcgc cgctcccaca tcgcccccgc tcaaagccgg
     2761 gaacaacatt cagcccattt cggatgagga aatcggtgcg caatgcatcc tccgtgatag
     2821 gttctcctcg gactctcgcc gggtgaaatg atgtcctttc cccgcggccg aggtcgagga
     2881 gatcaggggg gcgatggagc ggcggaaaat cagggaaagg gttcgggagc tcgctgccca
     2941 gggcggcgaa tggatttacg acatcccgtt gccccacggg gtgtggacgg gcgggaagct
     3001 caacgtccct cacacccggc tgaagcggat cgtccagatc atcggcgacc tgatggacaa
     3061 gccgctgtcg cgatgcaggg tcctcgacct cggctcgctg gaaggcttgt tctcgatcga
     3121 aatggcccag cagggggccg agacggtcgg agtcgagatc cgccggcaga atctcgacaa
     3181 ggcggagttc gcgagaaagc ttctcggcct gaccaacctc cagttccggc aagacgacgt
     3241 ccgcaacata tccgtcgccg gttatggcag gttcgatgcc atcatctgca gcgggatcct
     3301 ctaccatctg ccggcggggg acgtcatcga tctcatccgc accatgtatg ccatggcgga
     3361 ccgggccgtc gtcatcgata cccacgtcgc cctcgccgcg gaacgagcct ttacccgcgg
     3421 aggagaaacc tatcggggcc gcgaccattc cgagttcggt ccgggcatga gcgacgcgca
     3481 aaaggccgct tcccggctgt cgtcggcgga caacgacaca agcttctggc tgactcgtcc
     3541 ctcgctgatc aacgcaatgt cggccgccgg tttctccagc gtctacgaat gcttcacccc
     3601 cgcccacctg aactttggcc gccccggctt cgagagccgg gatcgggtga ctttcgtcgg
     3661 gatcaagggc gagaaggtca ggctgaacaa ctctcccgcc gccaacgatc tggatgagcg
     3721 gtggcccgag ggggcgctct cctatgcgcc gccgtcgaac tcgcttggga ggagaggcaa
     3781 gaatctggtc aagcgcctgc tcggccgctg atcccgccca ttcgatcgtc ggagtaaccg
     3841 gccgacgatc tcctaagtcc tcccggagct tgtcggaaag gggggacagg cgaagcccgg
     3901 tggaggggca atgccgagtc cgggctgccc tccaccacgc ttcgctcggc gcacttaccc
     3961 cgaaattgca gggcaggaat tcagatcacc gccccgacat tgtcgtcgga attacggtcg
     4021 atgtagaaat tgtaggggtc gatcccggcg tgggtcggct tgcggtcggt gacgaagcgg
     4081 aagacctgcc tgccgctctt caccggctgg cgctgcatcg tcaacacatt gctgcggtcg
     4141 aacgcgccac tccccggcgc cgctgtgaac aggccgatcg cgatccggtc ggcgaaggcg
     4201 gcgggcttct cgttgccctg gccgtcggca taggccttct tcgcctcgac cgggacgacg
     4261 acgtcccatt tgccgtccgc ccgcttcgtc gccttcgcct cgccgacctt gaggtcgtag
     4321 acggtgatcc gctcgaacag gtcggtgatc agcgcctggt cctctggtga cttcgcctcc
     4381 cggcggaagg cttcgaccag ctcgatcgag cgcggatagg gcgccccctt gaacttgtac
     4441 ttctcgagga cggagcggat ggcgcggttg accgcggctt cgccaagcct gtcctgaagc
     4501 aggtacatga ccagcgagcc cttgttgtag tggatatatt gctggttctc gacccgcatc
     4561 agcggcagct cctcgaccag ctcgccgccg cgaagccgaa ggtaggagtc gagctcgtat
     4621 ttgaggaaac ggcggatctt gtcgtcgccg taaagctgct tcatcaccat cagcgccgaa
     4681 tattgcgcga gagtctcgga gagcatggtt ccgccctgca tctccgcgcc gatgatctgg
     4741 tgaccccaat attgatggcc gagctcgtga gcggttatgt aggtgacgta gtcgatcttc
     4801 tcgggattgg acgtgtcggc gacgaagccg attgcctccg aataaggcat ggtgttcgcg
     4861 acggcctgag cgaagcggga gtaagccggg aactcgatga tccgcgcctg gtcgaactga
     4921 taggggccga acgcggtctg gtaatagtcc atggcaccgg ccagcgcgtc gagcatccgg
     4981 tcgacgttcc tgtcgtgcgt tgggtggtaa tagacggcga gatcgatgcc gcgatggttg
     5041 cggtgcttct cggcataacg cgccgactgg atggagaaga aggtcaggat cggagcgtcc
     5101 gagacgaagc gggccgtccg gcgccccccg ccgacgacgt cggacacctt cttgcccgga
     5161 gcgatcggaa tctggtccgc ctcggtcgag acggtgatgt ccgccgtcga ccagccgccg
     5221 ccgaaatagc tcttccgggt cgccgagagg tcctccagct tggcagcgcg ctggatgggc
     5281 ggaaggccgt atttccggcg cttggagcga tcctgaagca tccctccccg gtccattccg
     5341 atcgcgggca ggatctccat gttgttgagg aaggtgccgt tgcggaccaa ttttatgtcg
     5401 tggcccgaat tgcggaagcc ccggctccag cggcgggtct ggaagctgag ggcccgggtc
     5461 tcgcccggct gcatcggagt gtcgagcgcg tagatccggt agccatattc cttgtcgtcc
     5521 aggcgcagcc tggcgccggg gaagtcgagt cggagcagct ccaggtcgtt gggcggcatc
     5581 cgcacatgga cgtcgcggat cggagccgag gtcaggttcg agatgacgta gcgcccgtcg
     5641 atcacggcgc gggtctcgct gggatagagg gcgacgttca gcaccacctt cgagatggcc
     5701 gggtgcggca gggcctcgtt cttgaggtat ttcttctcat attcggccga aaacttgtcg
     5761 cggtcgctgc tcgtctcgaa ggtgttgatg acgttggtgt tgtaccaggc ataggcgccg
     5821 gtcgcgacga acacggtcag ggccagcccg gcgatgattc cgggtgcacc ggcgaggcgg
     5881 cgcggcgctc gccgaagccg gggcttgaga cgggtttcgg tgccccgccg ccagagcagg
     5941 tgggaggcga ccagaaggag ggttgcgaaa gccgcccagt agagcctgaa ccaccacgcg
     6001 ccgaccgcga agccgccgcc gccgttcatg tcggacaagg ggacgttggg cgacccgtta
     6061 tattggtaga gattgtgctc gaagcccatc gagacgagcg tgatggttgc cacgatgtag
     6121 accaccatca ccgcccagcc gacatatttg ttcgggctga tcgactggac gaacacggcg
     6181 agcaccgcga tcatcgtcac ttccacgccc agcggcagca ggtaccagag gagatatttg
     6241 ccgagctcga tgtcggtata gcccttgccg agctggacga ggatcgctgc gaccatcccg
     6301 aacaggaggg tggcgaagag gacgagggtg accgccaggg tctttgggac gacataggcc
     6361 cagttcggaa gcggggtcgc atcgatgatc tcgtgcatgc gccggtcgcg ttcccgcc
//
LOCUS       DASWNU010000036         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_493976, whole genome shotgun sequence.
ACCESSION   DASWNU010000036 DASWNU010000000
VERSION     DASWNU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493976"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..444)
                     /locus_tag="VGB57_00750"
     CDS             complement(43..444)
                     /locus_tag="VGB57_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0129903.1"
                     /translation="MKGYCDDIEKATVGNEDFRRVLYTGKNLQLVLMTLPPGCDIGEE
                     VHEDRDQFFRIEEGRGTVYIDGAPNKVEDDFAVIVPAGARHNVVNDGDSPLRLYTIYG
                     PPEHKDGVVHRDKAQAEADHDNDHWDGETSE"
     gene            complement(572..1150)
                     /locus_tag="VGB57_00755"
     CDS             complement(572..1150)
                     /locus_tag="VGB57_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129904.1"
                     /translation="MEPLYYSRGRLAAAATFFLVVGGFFVWLWMDPSWAAASRRFGRL
                     LASDFGRYVLIPSCAAVCLAVSGLTASLVLGNGKALESSGTHLIVSTVWGRKRIALSE
                     LRSFGIERSGRYPQLVLEGDGIGILGGSTLRIALGTVQDGESRIPSLLDTLAALRAEA
                     LRPRLDRASAAPAASAPPLPLPQRAAFGRKGS"
     gene            1354..1839
                     /locus_tag="VGB57_00760"
     CDS             1354..1839
                     /locus_tag="VGB57_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0129905.1"
                     /translation="MEDWKLPWEGGCRCGGLRVRVTKPPLLAGACHCAGCQKMTASAF
                     SLSLALPADGFEVTKGEPVLGGLRGPVSHHHHCPACLSWVFTRVEGMDWFVNLRASAL
                     DDRGWFGPFMEVWTDEKLPWASTGARHSYGKAPEMADFEPLMAAYAAEGARPRGRLTP
                     G"
     gene            complement(1826..2488)
                     /locus_tag="VGB57_00765"
     CDS             complement(1826..2488)
                     /locus_tag="VGB57_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY0129906.1"
                     /translation="MGFAAGVMLAASFFSLIIPGIEQGQSSGFGRGPAAAIVIVAMLA
                     GASFVGLMNRMVPALDRIGLSPNRGIGAAKHRRICLFVAAITLHNFPEGLAVGVSFGA
                     GDFKAGYATALGIGLQNIPEGLTVAAALMTIGYERGRAILAALASGLVEPVGGFVGVA
                     TVAAVASLLPWALGFAAGAMIYVVCSEIIPHAHREDEGGTATTGLMLGLAAMMFLDIS
                     LG"
BASE COUNT          422 a          915 c          924 g          421 t
ORIGIN      
        1 ttcttcgtct ggctggggcg gcggctcgac agccgcgcct gatcactcgc tcgtctcgcc
       61 gtcccagtga tcattgtcgt gatcggcctc ggcctgagcc ttgtcgcggt ggaccacccc
      121 gtccttgtgc tccggcgggc cgtagatcgt gtacagcctg agcggcgagt cgccgtcgtt
      181 gacgacgtta tggcgcgccc cggcgggaac gatcacggcg aaatcgtcct ccaccttgtt
      241 cggggcgccg tcgatataga cggtcccgcg cccttcctcg atccggaaga actggtcgcg
      301 gtcctcatga acctcctcgc cgatgtcgca gcccggcggc agggtcatca gcaccagctg
      361 caggttcttg ccggtataga gcacccgccg gaaatcctcg ttcccgacgg tggccttctc
      421 gatgtcgtcg caatagccct tcatgtgctc gagctcctcc gcttggatag tcgagcggtg
      481 aaccgcgatg ggcgccattg cgttccttac agcgagtcgc gagccgtggt tcggcccgtc
      541 gggatggggt gcgtccggcc agtgcgcgcc ttcagcttcc cttgcggccg aacgcggccc
      601 gctgcggcag cggcagcggc ggagcggaag cggccggcgc ggccgacgcc ctgtcgagcc
      661 tgggccgaag cgcctccgcc ctcagggcgg cgagcgtgtc gagcaggctc gggatccggc
      721 tctctccgtc ctgcaccgtc cccagcgcga tccgaagcgt cgagccgccc aggatgccga
      781 tcccgtcgcc ttccagaacg agctgcgggt agcggccgct tcgctcgatg ccgaaggatc
      841 gaagctccga cagggcgatc cgcttgcggc cccagacggt ggagacgatg aggtgggtcc
      901 cggacgattc cagggccttg ccgttgccca ggacgaggct cgcggtgagg cccgacaccg
      961 cgaggcagac ggcggcgcag gacgggatga ggacgtaccg gccgaaatcg cttgcgagca
     1021 gccggccgaa gcgacgcgag gcggcggccc agctcgggtc catccacagc cagacgaaga
     1081 agcctcccac gacgaggaag aaggtcgcgg ccgcggcaag ccgcccgcgg ctgtagtaaa
     1141 gcggctccat ctccgtcccc ccgcaaaatg ccgaacgggg atagcgcggc tcggctaaag
     1201 tcctgttaac gcccgccctc gctgccccgc tcgcccgggc gcccgacagt cgctagtgag
     1261 tcgctagtga cagtcactag tgacagtcac tagtgacagt cacttttggc ctagtgactg
     1321 tcacttttgg cacttttggc gcgggaggcg gagttggaag attggaaact gccctgggag
     1381 ggcggctgcc gctgcggcgg gctgcgcgtg cgggtgacga agccgccgct tctcgccggc
     1441 gcctgccact gcgccggctg ccagaagatg accgccagcg ccttttccct cagcctggcg
     1501 ttgcccgccg acggtttcga ggtgacgaag ggcgagccgg tcctcggcgg cctgcgcggt
     1561 ccggtgtcgc accatcatca ctgcccggcc tgcctgagct gggtgttcac ccgggtcgag
     1621 ggcatggact ggttcgtcaa cctgcgcgcc tccgcgctcg acgatcgcgg ctggttcgga
     1681 ccgttcatgg aagtctggac cgacgagaag cttccctggg cttcgaccgg ggcgcggcac
     1741 agctacggca aggcgcccga gatggcggac ttcgaacccc tcatggccgc ttatgccgcc
     1801 gagggcgccc ggcctcgggg ccgcctcacc ccaggctgat gtccaggaac atcatcgcgg
     1861 cgagcccgag catcagtccg gtcgtcgcgg ttccgccttc gtcttcccgg tgggcgtggg
     1921 gaatgatctc tgaacagacg acatagatca tcgcgccggc ggcgaagccg agcgcccagg
     1981 gcagaaggct cgcgacggcg gcgaccgtgg cgactccgac gaagcccccg accggttcga
     2041 ccaggccgga ggcgagggcg gcgaggattg cgcgaccgcg ctcatagccg atggtcatca
     2101 gcgcggcggc gacggtgagg ccttccggaa tgttctgaag gccgatgccc agggcggtgg
     2161 cataacccgc cttgaagtcg ccggcgccga agctgactcc gaccgccagg ccttccggga
     2221 agttgtgaag cgtgatcgca gcgacgaaca ggcagatccg gcgatgcttc gccgcaccga
     2281 tgccccgatt cggggaaagc ccgatcctgt cgagcgccgg gaccatccgg ttcatcaggc
     2341 cgacgaacga ggcgccggcc agcatcgcta cgatgacgat cgccgccgcg gggccgcggc
     2401 cgaagccgct cgactgcccc tgctcgatgc ccggaatgat cagcgaaaag aacgaagcgg
     2461 cgagcatcac ccccgcggcg aaccccatca agccgttttc caggcgcacg cccggcttgc
     2521 ccgtcccgaa cagggggagg gcgccgagaa cggtcgcgag accggcggcc aggctgccga
     2581 ggaagccggt gagtatgatc gaatatatca gtgcgtccgc ctaagtccgg tcgatccgtg
     2641 gtgcgggacg cggccggcct cggccgcgtc ccgcaggcgc tt
//
LOCUS       DASWNU010000037         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_416876, whole genome shotgun sequence.
ACCESSION   DASWNU010000037 DASWNU010000000
VERSION     DASWNU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416876"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGB57_00770"
     CDS             <1..673
                     /locus_tag="VGB57_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0129907.1"
                     /translation="WLPQNLGSKYVIVNVPAYTVALVENGQTLNRRRAVAGAIKTPTP
                     SLSAVITGAIFNPWWEVPSSIASEVRGKKGYVAVKDDSGKIIRWRQPPGPSNALGRMK
                     LVMGNPHAIYLHDTNAKNLFNRQARAFSHGCIRTEDAVGFAETLLDGTEWDRGKIDQT
                     LASGKATQANLAAPVPVYIVYFTVAATSDGPPNSYVDLYGRDGKVLAGLSDRKAGTAL
                     ASSGQ"
     gene            878..2578
                     /locus_tag="VGB57_00775"
     CDS             878..2578
                     /locus_tag="VGB57_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0129908.1"
                     /translation="MDAFSALFRLLSPARRRQFVLTLLLMLVGAAAEMVTIGAALPFL
                     ALVADPRSALIPPRLLGVLGAIGGSPVVGAALLLIAAALLAAAVRLALTWGSQRFVMA
                     AGHDMAASVFGRMLRQPYAAQVRRNSSQTLAAIEKVQGVVFGLLQPAMQGMIGAFIAL
                     CVFALLLRIDARAAGLAAFSVALVYLGISLLVRPRLRRNSEMLAGTMVERTRTVQEGL
                     GGIRDIILDRSEPLFEAKFRELDSRYRRAQAATQFVAGAPRFVVEAAGLIAIALVTLT
                     MSLEPGGVVKAIPVLGALALGAQRLLPLLQQTYHGWSLASGNYRAFRDVIEMMEAPIP
                     PPQPEAPPLPIERELAFEGVGFRYPEGRFALADVSFRIRAGEHVGIAGATGGGKSTLL
                     DLLMGLLEPDEGAILVDGRRLDAATRPAWQAGLAHVPQAIYLADDTIAANIAFPRGAD
                     GMDPELLAAAIRAAQLETFLAGLPEGLRTFVGERGVRLSGGQRQRIGLARALYRRPRL
                     LILDEATSALDETTEKAVLAALQGLRKDMTLVTVAHRATTLAACDRLIRVEGGRVSEG
                     "
BASE COUNT          390 a          978 c          906 g          411 t
ORIGIN      
        1 ctggcttccg cagaatctcg gctccaaata tgtgatcgtc aacgttccgg cctacacggt
       61 cgcgctggtc gagaacggcc agaccctgaa ccgccgccgg gcggtggcgg gcgcgatcaa
      121 gaccccgacg ccgagcctgt cggcggtcat caccggcgca atcttcaacc cctggtggga
      181 agtgccctcc agcatcgcct ccgaggtgcg cggcaagaag ggctatgtcg cggtcaagga
      241 cgacagcggc aagatcatcc gctggcgcca gcccccgggg ccctccaatg cgctcggccg
      301 gatgaagctg gtgatgggca atccccatgc catctacctc cacgacacca atgcgaagaa
      361 cctgttcaac cggcaggcgc gggccttcag ccacggctgc atccggaccg aggacgcggt
      421 cggcttcgcc gagaccctgc tcgacggcac cgagtgggac cgcggcaaga tcgaccagac
      481 cctggcgtcg ggcaaggcca cgcaggccaa tctcgcggcg ccggtgccgg tctacatcgt
      541 ctacttcacc gtcgccgcga ccagcgacgg cccgccgaat agctacgtcg acctgtacgg
      601 ccgcgacggc aaggtgctgg ccgggctcag cgaccgcaag gcgggcacgg cgctggcttc
      661 gtcagggcag taaaggcact tctgcctccc catcgccaga tggggagggg gaccatgcga
      721 agcatggtgg aggggtagat ggcggcggag ctttcggttc gcgcgagaac ctccttgaac
      781 tgagccaatt acccctccac cacccttcgg gtggcccccc tccccatcgc ttcgcgacag
      841 ggaggcaagg gggtgccgga ggaaccttac tcttcacatg gacgcattct ccgccctttt
      901 ccggctgctc tcgcccgctc ggcggcggca gttcgtgctg accctgctgc tcatgcttgt
      961 cggggcggcg gcggagatgg tgacgatcgg cgcggccctg cccttcctcg ccctggtcgc
     1021 ggacccccgg agcgcgctca ttccgccgcg gctgctcggg gtgctgggag cgatcggcgg
     1081 ctcgccggtg gtcggcgccg ccctgctgct catcgccgcg gccctgctcg cggcggcggt
     1141 tcgcctcgcc ctcacctggg gcagccagcg cttcgtcatg gcggcgggcc acgacatggc
     1201 cgcttccgtc ttcgggcgaa tgctccgcca gccttatgcg gcgcaggtgc ggcgcaactc
     1261 cagccagacc ctcgccgcga tcgaaaaggt gcagggcgtc gtcttcggcc tgctccagcc
     1321 ggcgatgcag ggcatgatcg gcgccttcat cgcgctctgc gtcttcgccc tgctgcttcg
     1381 aatcgacgcc cgcgccgccg ggctggccgc cttttcggtg gccctggtct atctcgggat
     1441 cagcctgctc gtccggcccc ggctgaggcg caattccgaa atgctggctg gaacgatggt
     1501 cgagcggacc cgcaccgtcc aggaggggct gggcggaatc agggacatca tcctcgaccg
     1561 ctccgagccg ctgttcgaag ccaagttccg ggagctcgac agccgctacc gccgggccca
     1621 ggcggcgacc cagttcgtcg ccggagcccc gcgcttcgtc gtcgaggcgg cggggctgat
     1681 cgcgatcgcc ctggtcaccc tgaccatgag cctggagccg ggcggggtgg tcaaggccat
     1741 cccggtgctc ggcgctctgg ccctgggcgc ccagcggctg cttcccctgc tccagcagac
     1801 ctatcacggc tggagcctgg cgagcggcaa ttaccgcgcc ttccgcgacg tcatcgagat
     1861 gatggaagcg ccaatcccgc cgccgcaacc ggaggcgccg ccgctcccca tcgagcgcga
     1921 gctggcattc gagggggtgg gcttccgcta tcccgaaggc cgcttcgcgc tcgccgatgt
     1981 cagcttcagg atccgcgccg gcgagcatgt cgggatcgcg ggcgcgactg ggggcggcaa
     2041 gtcgaccctg ctcgacctgc tgatgggcct gctcgagccc gatgagggcg cgatcctggt
     2101 cgacggccgg cgcctcgacg cggcgacccg ccccgcctgg caggccggcc tcgcccacgt
     2161 cccccaggcc atctacctcg ccgacgacac gatcgccgcc aacatcgcct ttccgcgcgg
     2221 cgccgacggg atggacccgg agcttctcgc cgccgccatc cgggcagcgc agctcgagac
     2281 cttcctcgcc ggattgcccg aggggctccg gaccttcgtc ggcgagcgcg gcgtccgact
     2341 gtccggaggc cagcgccagc gcatcggcct cgcccgggcc ctctaccgcc ggccgcgcct
     2401 gctcatcctc gacgaggcga ccagcgcgct cgacgagacc accgagaaag cggtcctcgc
     2461 ggcgctccag gggcttcgga aggacatgac cctggtgacg gtcgcgcatc gcgcgacgac
     2521 cctggcggcc tgcgaccggc tgatccgggt cgaaggggga cgggtgagcg agggctgata
     2581 tcctccccat gcgcagcatg gggaggggga ccaggcgaag cctggtggag gggcaactgg
     2641 aacaggtctg ctccagctac ccctccacca cccttcgggt aatcc
//
LOCUS       DASWNU010000038        10465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_78987, whole genome shotgun sequence.
ACCESSION   DASWNU010000038 DASWNU010000000
VERSION     DASWNU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78987"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="VGB57_00780"
     CDS             <1..1866
                     /locus_tag="VGB57_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0129909.1"
                     /translation="ITGFGTGGSGVGGLGGLSGTTVASVGGEKLTSTRLSDELQRQLT
                     RLRTQQPELDMAAFLRQGALEEVLEQLVDLTASAVFGREQGLGVSRQMIDRDIASIPE
                     FHDLTGRFDDATFRRVLAQENLSEQQVRDDFATRLLQRQLLGPIARSAHVPNAIALQY
                     ASLLLESRTGIVGAVPSAAVGAGGEPSEAELAAFYRTARARYTIPERRVIRYAAFGPE
                     NVAAQSQATDAEIQAAYRSDPAYAARETRRLSQVVLPEAEARALAQKVAAGTAFAAAA
                     AQAGRNAADIALGANSREEYAAKSSPAVAAAVFGAAKGATVGPIRGPFGWHVVKVEDV
                     VVSAARPLAAVRDELAAQIQQRKAANALNELAGRIETAISDGSTFDEIAAANRLAVKE
                     TAPVTATGAAPGTPGWKAPPELQPLLEGAFAMEPGEDPAVETIQENQRYALIAVARAV
                     PAAVPPLASIRDRVKADLIVRRASERARAVASSIVARINAGTPPSQAFASAQVKLPPV
                     QTLTATRREIARANAQVPPPMAMMFSLPRGKARLLPAPDGRGWFVVYLDRIVPGDAGK
                     EPALVQAVRNQFAAIIGEEYARQFTTAIRDDLKIERNEEALRKLKADLAGPGAAQ"
     gene            1892..2470
                     /locus_tag="VGB57_00785"
     CDS             1892..2470
                     /locus_tag="VGB57_00785"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY0129910.1"
                     /translation="MILVLDNYDSFTWNIVHYLRELGTQVRVERNDALTAAEALATGA
                     AAFVISPGPGRPEGAGASLDLVAACAQARRPLLGICLGHQAVAQHFGAEIVRAEAVMH
                     GKTCAVSHEGTGLFRGLPSPLTVTRYHSLTVAPQSVPDCLAANARADDGTVQGLRHRT
                     LPIHCVQFHPESVASEWGHELLGNFVGLASAG"
     gene            complement(2560..3213)
                     /locus_tag="VGB57_00790"
     CDS             complement(2560..3213)
                     /locus_tag="VGB57_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129911.1"
                     /translation="MGSHTTIDIIKTVAPVALNALLASAEKLASGSNPQRAMADAFIA
                     AANAVAGKNVVARVALDDPAPSSTVLSDLSEAGANLWQDRFDYFYQGNGSMCLAGDKT
                     VRASNSGSDLVIYDESQGQMPDIKGYITSVFKDTALSDQATIELVNNLTTIIHARWTE
                     ESLDWTPLTKTYASLPLSGGMNVDMLLYTACATDNSGNGTLAGLAFYMFTYYQTPGS"
     gene            3589..4245
                     /gene="lexA"
                     /locus_tag="VGB57_00795"
     CDS             3589..4245
                     /gene="lexA"
                     /locus_tag="VGB57_00795"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0129912.1"
                     /translation="MLTRKQSELLTYIQARLAESGVSPSFEEMKEALALKSKSGVHRL
                     ISALEERGFIRRLPNRARALEILRMPEGQAAGAAAQPKAPAPSLSAANDVIDLPLHGR
                     IAAGMPIEALEGQTSLPVPAALLGPGEHYALEVAGDSMVEEGILDGDYALIRKTDMAR
                     DGEIVVALIDDNEATLKTYRKEGQMVRLDPANRRYDPQRYRPEQVRIQGRLAGLLRRY
                     "
     gene            4383..4721
                     /locus_tag="VGB57_00800"
     CDS             4383..4721
                     /locus_tag="VGB57_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0129913.1"
                     /translation="MARLNYVELPVSDTGTARAFYEAAFGWSLTDFGPTYAATLTGDT
                     DLGLQGDPAEAARAPLPVIDVADLEAALAAVESAGGRIVRPIFAFPGGRRFHFLDPSG
                     NELAAVKAGH"
     gene            4919..4994
                     /locus_tag="VGB57_00805"
     tRNA            4919..4994
                     /locus_tag="VGB57_00805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4952..4954,aa:Lys,seq:ctt)
     gene            complement(5059..5685)
                     /locus_tag="VGB57_00810"
     CDS             complement(5059..5685)
                     /locus_tag="VGB57_00810"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY0129914.1"
                     /translation="MAFELTPLPYASDALGEFMSAETLAFHHGKHHRAYVDKVNAILA
                     DRPDPGGSLVEIVRAAKARGESALFNNSAQIWNHNFYWRCLAPAQGRKPAGRLAEMID
                     DGFGGVGNLLLRLKEEATGHFSNGWAWLVLDGGRLAITSLHDADSPVVHEGMKPLLTL
                     DVWEHAYYIDYRNARADFAEKVLGNIVDWDFVERNLDGEGVGRGDQER"
     gene            5844..7097
                     /locus_tag="VGB57_00815"
     CDS             5844..7097
                     /locus_tag="VGB57_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F390 synthetase-related protein"
                     /protein_id="HEY0129915.1"
                     /translation="MLDVAHAATAFLRTRALQALLRSRGAIERHQRRGCARLVSAARA
                     TIPFYRDEGPRFEDLPVIDKAIQQARFHEFNRPGIALDEAVEALAAGRDRVRGYFVGQ
                     STGTSGNRGRFVISPAERFAWLGTIVAKAVPDALVRRRRVALALPGFSSLYKSASSGS
                     RIRLAFFDLSLGVDAWRDDLAAFAPEVLVAPPKVLRRLAEDGMLEGVEPFSGAEVLDP
                     IDREIVEQASGRRVREIYMATEGLFAVACRHGTLHLAEDVVRFEWEKAPGSDLVIPVV
                     TDMVRRIQPMIRYRMNDLLELARIPCPCGSPFQAIARIHGRMDDVFEFEAPLGRVMAT
                     PDVLRNAVVDSDPRVRDYRIVQTRDGGVKVELDEALPDEVHSAVRAALERALARLGAA
                     PAIATVRGITPRFDRKLRRVERQRA"
     gene            complement(7094..8089)
                     /locus_tag="VGB57_00820"
     CDS             complement(7094..8089)
                     /locus_tag="VGB57_00820"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0129916.1"
                     /translation="MVGAWPAAIASGWHPIAFSPAVGARPVAARLMGRRLVVARDGDG
                     PFVLDDRCPHRGAPLSRGRVEAGAVICPYHGWRFGRGGACLAVPGSPLPGAPAKALPC
                     VDRAGLVWTSLAEAPDPFPRLPGAMEDESLDRFWWPLRATPAGLLDAIENHLDPAHPH
                     HVHRWMVRSPNRRRPVQVSVRSGPWGAEAAYLEGSTPAGLMPRLLEGRRVRSLGRFYP
                     PTIAEVAFEGEAELKLSVAVVFGPEDETLTRPWAHFATRRGLAPAWLKRAVLKAFHLP
                     VLNQDRRILRLQQEWRQGEAPSYATGPLDVMGPTIWRLANGLAEPERERSLTMDL"
     gene            complement(8083..9177)
                     /locus_tag="VGB57_00825"
     CDS             complement(8083..9177)
                     /locus_tag="VGB57_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129917.1"
                     /translation="MEWRGLRVTAAMLREMVESGAPSMVSNVSTRFSTLDVAGRTMPV
                     TVDDGALGGSYVCSPHSAYVLYARAELDLLRVGWTAWPSRAALAALDLVLKAGRINRI
                     VHLGNWLLSTNLHGDWRGEGVKAAREALAGAHPDHLLGIRSIDDWSAPGLREALAADG
                     WIMVPSRQIWVVDDLGRDWTKRSDFANDRRLVERSPLTLSELARVEADDAERIAQLYA
                     MLYVGKYSPLNPILTPAFIRASARSGMMSYRVARDPRGTIQAAAGMWERGGVMTPPVV
                     GYDTARPQREGLYRIACWMFMQRALQAGWRLHGSAGAAHFKRMRGARGVIEWNAYHAG
                     HLPAARRRPVEALAMLLKRLVVPVMVAKQW"
     gene            complement(9153..10298)
                     /locus_tag="VGB57_00830"
     CDS             complement(9153..10298)
                     /locus_tag="VGB57_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129918.1"
                     /translation="MTRVMITGARAPVALDLARSFRSAGCEVALADSVRPFAASLSRP
                     RFEILRLPPPRRHFPAFRARLSALAERYELIVPTCEEVFWLAAAARSDGWSDRLFAPS
                     IESLRALHSKASFPALALEAGVEAPATWAIASAADANLVPLAPADLVLKPEFSRFGSK
                     TIVGPQAGMAAALPASADRRWVAQERLDGEELCVWSAVRGGRLVACIVYRPALRHGRS
                     AAFAFEAVDEPQVVEMARRIAVHVGGDGQLSFDVIVQPGGRIAPIECNPRAVSGVHLM
                     DGSPALALAMLGRGELPPPPAGTMRYLSPAMALMGVPAALADGTFGRLRAVWRKGRDA
                     VGKPGDRLPVAGVLLDAARFALMGAARLHGPTSETTDDIEWNGEAFE"
BASE COUNT         1607 a         3696 c         3619 g         1543 t
ORIGIN      
        1 atcaccggct tcggcaccgg cggctccggc gtgggcgggc tcggcggcct gagcggaacc
       61 acggtcgcca gcgtcggcgg cgagaagctg acctcgaccc ggctgagcga cgagctccag
      121 cggcagctga cccgcctgcg cacccagcag cccgagctcg atatggccgc cttcctccgg
      181 cagggcgcgc tcgaggaggt gctcgagcag ctcgtcgacc tcaccgcgag cgccgtcttc
      241 ggccgcgagc aggggctcgg agtgtcgagg cagatgatcg accgcgacat cgcgtctatc
      301 cccgagttcc acgatctcac cggccgcttc gacgacgcca ccttccggcg agtgctcgcc
      361 caggagaatc tgagcgagca gcaggtccgc gacgatttcg cgacacgctt gctccagcgc
      421 cagctgctcg gcccgatcgc gcgctcggcc cacgtcccca acgcgatcgc gctccaatat
      481 gcctcgcttc tgctcgaatc gcgcaccggc atcgtcgggg cggtgccgtc ggcggcggtg
      541 ggcgccggag gggagccgag cgaggccgag ctcgccgcct tctaccggac cgcccgggcc
      601 cgctacacca tcccggagcg gcgggtgata cgctatgccg ccttcggccc ggagaatgtg
      661 gcggcgcagt cccaggcgac cgatgccgag atccaggccg cctatcgttc cgacccggct
      721 tatgccgcgc gcgagacccg ccggctgtcg caggtcgtgc ttcccgaagc ggaggcgcgg
      781 gcgctggcgc agaaggtcgc ggccggcacc gccttcgccg ccgccgcggc ccaggccgga
      841 cgaaacgccg ccgacatcgc gctcggcgcc aacagccgcg aggaatatgc ggcgaagagc
      901 tcgcccgcgg tggccgcggc cgtgttcggc gccgccaagg gcgcgacggt ggggccgatc
      961 cgcggcccct tcggctggca tgtcgtcaag gtggaggacg tcgtggtttc ggccgccagg
     1021 ccgctggccg cggtgcgcga cgagctggcc gcccagatcc agcagcgcaa ggcggcgaac
     1081 gccctcaacg agctcgccgg gagaatcgag accgcgatca gcgacggttc gaccttcgac
     1141 gagatcgccg ccgccaacag gctggcggtg aaggagaccg ctccggtcac cgcgaccggg
     1201 gccgctccgg gcacccccgg ctggaaggcg ccgcccgagc tccagcccct gctcgaaggg
     1261 gccttcgcga tggagccggg cgaggatccg gcggtcgaga cgatccagga gaatcagcgt
     1321 tacgcgctga tcgccgtcgc ccgggcggtt ccggccgcgg tgccgccctt ggcctcgatc
     1381 cgcgaccggg tgaaggccga tctgatcgtc cgccgggcgt ccgagcgggc gcgggcggtg
     1441 gcgagctcga tcgtcgcccg gatcaacgcc gggaccccgc cctcccaggc cttcgcttcg
     1501 gcgcaggtga agctgccgcc ggtgcagacg ctcaccgcca cccgccgcga gatcgcgcga
     1561 gccaacgccc aggtgccgcc gccgatggcg atgatgttca gcctgccccg cggcaaggcg
     1621 cgcctgctgc cggcgcccga cgggcgcggc tggttcgtcg tctatctcga caggatcgtc
     1681 cccggcgatg ccggcaagga gcccgcgctg gtccaggcgg tgcgaaacca gttcgcagcg
     1741 atcatcggcg aggaatatgc ccgccagttc accaccgcga tccgggacga tttgaagatc
     1801 gagcgcaacg aggaggcgct tcgcaagctc aaggccgatc tggcggggcc cggcgcggcc
     1861 cagtgacggg cggccgggag aaggatcgcg aatgatcctc gtcctcgaca attacgacag
     1921 cttcacctgg aatatcgtcc attatctgcg cgagctcggg acccaggtgc gggtcgagcg
     1981 caacgatgcc ctgaccgccg ccgaggcgct cgcgaccggg gcggccgcat tcgtgatctc
     2041 gcccgggccg ggccggccgg agggcgccgg ggcttcgctc gacctggtcg ccgcctgcgc
     2101 ccaggcccgg cggcccctgc tcgggatctg cctcggccac caggcggtgg cgcagcattt
     2161 cggagccgag atcgtccggg cggaggcggt gatgcacggc aagacctgcg ccgtcagcca
     2221 tgagggcacc gggcttttcc gaggcctccc ctcgcccctg accgtgaccc gctaccattc
     2281 gctcaccgtg gcgccgcaaa gcgttccgga ttgcctcgcc gccaacgcgc gcgcggacga
     2341 cggcaccgtc cagggccttc gccaccgcac tctgcccatc cactgcgtgc aattccatcc
     2401 cgagagcgtg gccagcgagt gggggcatga acttctcggc aatttcgtcg ggctggcatc
     2461 ggcgggctga ggggaagacg agcgcgcgtc gcctcgacgg ccggcggccg ggactccggc
     2521 cggggcggaa ccggtccgcc ccgtgccatt cccagccgat caggagccgg gcgtctgata
     2581 ataagtgaac atgtaaaaag cgagcccggc cagggtcccg ttgccgctat tgtcggtggc
     2641 gcaggcagta taaagcagca tgtcgacgtt catgccgccg ctcaagggca gcgacgcata
     2701 ggtcttcgtc agcggtgtcc agtccaggct ttcctccgtc cagcgagcgt ggatgatggt
     2761 ggtgaggttg ttcaccagtt cgatcgtcgc ctgatccgaa agcgccgtgt ccttgaatac
     2821 actggtgatg tagcccttga tgtcgggcat ctggccttgc gactcgtcgt agattacgag
     2881 atccgatccc gagttggacg cacgaacggt tttgtcaccg gccagacaca tcgaaccgtt
     2941 gccctgatag aaatagtcaa agcggtcttg ccacaagtta gccccagcct cacttaaatc
     3001 actcaaaacc gttgaactgg gcgctgggtc atccagcgcg actctcgcga cgacgttctt
     3061 gccagctaca gcgtttgctg ccgctataaa agcatcggcc attgctcttt gtgggtttga
     3121 tccggatgcc agcttttctg cggaggccaa caacgcattc aacgcaactg gcgcgacggt
     3181 cttgatgatg tctatagttg tatggctgcc catgttgact ctcctcgttg gcgtaggata
     3241 gcgacctcga ttcgggccga cacatgtatg actacgccag attccccctc tactagagtc
     3301 gaacctgctc cactttggga atattttgag tcattatgga tgatttcgag gcacaggttc
     3361 attgccgcca tgctcggcgg gctcaggttc gagcggcgac gcggcttcct gaaggctggg
     3421 atcaggcggc tcaaccgttc tccggccggt aaccagggcg gcccgggaaa cggccgccgg
     3481 ggcctatgcc gccgaaattt catcggaatc gcggattgtt ccggccccgc ttgacagctc
     3541 ccgcgacgtt ccctacacgt tcctcgttcg tttctcaggg gacccgatat gctgacgcgc
     3601 aagcagagtg agcttctgac ctacatccag gcccgcctgg ccgaaagcgg ggtttcgcct
     3661 tcgttcgagg aaatgaagga ggctctggcg ctcaaatcca agtcgggagt ccaccggctc
     3721 atctccgcgc tcgaggagcg cggcttcatc cgccggctgc ccaaccgggc ccgggcgctt
     3781 gaaatcctgc gcatgcccga aggccaggcc gccggcgccg ccgctcagcc caaggctccg
     3841 gcgccttcgc tttcggccgc caacgacgtc atcgacctgc cgctccacgg ccggatcgcc
     3901 gccggaatgc cgatcgaggc gctcgagggg cagaccagcc tgcccgtccc cgccgcactg
     3961 ctcggcccgg gtgagcatta tgcgctcgaa gtcgccggcg attcgatggt cgaggaaggc
     4021 attctcgacg gcgattacgc cctcatccgc aagaccgaca tggcgcgcga cggcgagatc
     4081 gtggtggcgc tcatcgacga caatgaggcg accttgaaga cctaccgcaa ggaaggccag
     4141 atggtccggc tcgatccggc caaccgccgc tacgacccgc agcgctaccg gcccgagcag
     4201 gtccggatcc agggccgcct ggcggggctg ctccgccgct attgacggac cgccgccctg
     4261 acgggcggcg gcacgtcatc ccggcggagg ccgggatccc ggcgggtgaa gggcgccagc
     4321 gcttttcacc ccccggggcc ccagcctttg ctggagcaac gaccagataa gggagtaacg
     4381 cgatggcacg cctcaactat gtcgagcttc cggtaagcga caccggcacg gccagggcct
     4441 tttacgaagc ggcgttcggc tggagtctga ccgatttcgg cccgacctat gccgcgacgc
     4501 tcaccggcga caccgatctc ggtcttcagg gcgatccggc ggaggccgcg cgggcgccgc
     4561 tgccggtgat cgacgtcgcc gacctcgagg cggccctggc cgcggtcgag tcggcgggcg
     4621 ggcgaatcgt ccgcccgatc ttcgccttcc ccggcggccg ccgcttccac ttcctcgacc
     4681 ccagcggcaa cgagctggcg gcggtaaaag cgggtcacta gccgtcgccc cggcgaaagc
     4741 gggcccagag ggtgaaatac gaggcggcgg gcacgggccg cggccccgaa atccgccttc
     4801 gccacctttt ccgctccgtg gccctggcct gcgccggggc gacgaggcag tttaagccgc
     4861 ctggtcggtt gcaatccgcc gccgccgcct ctatctgccc cctctgcccg acaggcacgg
     4921 acgtatagct cagttggtag agcagctgac tcttaatcag cgggtcccag gttcgagccc
     4981 tggtgcgtcc accatctttc tcaacccgaa ccgacgcgcg gacgctctgc cttgcggcgc
     5041 gtccccgccg agcggggctc agcgctcctg atcgcctcgg cccacgccct cgccgtcgag
     5101 gttgcgctcc acgaaatccc agtcgacgat gttgccgagc accttttccg cgaaatcggc
     5161 gcgggcgttg cgatagtcga tataataagc gtgctcccac acatcgagcg tcaggagcgg
     5221 cttcatcccc tcgtggacga ctgggctgtc cgcatcgtgg agcgaggtga tcgcgagcct
     5281 gcccccatcg aggaccagcc aggcccagcc gttcgagaaa tggccggtcg cttcctcctt
     5341 gagccggagc agcaggttcc cgaccccgcc gaaaccgtcg tcgatcatct cggccagccg
     5401 ccccgccggc ttccggccct gggcgggcgc gagacagcgc cagtagaaat tgtggttcca
     5461 gatctgcgcc gaattgttga acagcgcgga ttccccccgc gccttcgccg cccggacgat
     5521 ctcgacgagc gagcctcccg ggtccggccg gtcggccaga atggcgttga ccttgtcgac
     5581 ataggcccgg tggtgcttgc catggtggaa agcgagggtc tcggccgaca tgaactcgcc
     5641 gagggcatcg gaagcgtaag gcagcggggt gagctcgaac gccatcggta tctccgccag
     5701 ggcaatccgt caggccctcc ccaggcaaat gaatatggcg cgctttgtat ccccggcctg
     5761 actttggccg gagaagcgga tagcgtggag ggcggatcca ccccgggccg cggcggcggc
     5821 ctggccctcg cggaggcgct caattgctcg atgtcgctca tgccgcgacg gctttcctcc
     5881 ggacccgggc gctacaggcg ctgctgcgtt cgcggggcgc gatcgaacgg catcagcgac
     5941 gcggctgcgc acgcctggtc tcggcggccc gggcgaccat ccccttctac cgcgacgagg
     6001 gcccgcgctt cgaggacctg ccggtgatcg acaaggcgat ccagcaggcc cgcttccacg
     6061 agttcaaccg gcccgggatc gcgctcgacg aggcggtcga ggcgctggcg gccgggcgtg
     6121 accgggtgcg cggctatttc gtcgggcaga gcaccggcac cagcggcaat cgcggccgct
     6181 tcgtcatctc gccggccgag cgcttcgcct ggctgggaac gatcgtcgcc aaggcggtgc
     6241 cggacgccct ggtccggcgg cggcgggtgg cgctcgctct gccgggtttc tcgtcgctct
     6301 acaaatccgc ctcgagcggg agccggatca ggctcgcttt cttcgacctg tcgctcggag
     6361 tcgacgcctg gcgcgacgat ctcgccgcct tcgcccccga ggtgctggtg gcgccgccca
     6421 aggtgcttcg ccggctcgcc gaggacggaa tgctcgaggg agtcgagccg ttcagcggcg
     6481 ccgaggtcct cgacccgatc gaccgcgaga tcgtcgagca agcgagcgga aggcgcgtcc
     6541 gcgaaatcta catggcgacc gaggggctgt tcgcggtcgc ctgccggcac gggaccctcc
     6601 acctcgccga ggatgtggtg cggttcgagt gggagaaggc gccgggcagc gatctcgtca
     6661 tccccgtcgt caccgacatg gtgcgccgca tccagccgat gatccgctac cggatgaacg
     6721 atctcctcga gcttgcgaga atcccctgcc cctgcggctc ccccttccag gcgatcgccc
     6781 ggatccacgg gcggatggac gacgtcttcg agttcgaggc gccgctcgga cgggtgatgg
     6841 ccaccccgga cgtgcttcgc aacgccgtgg tcgattccga cccccgggtc cgcgactacc
     6901 ggatcgtgca gacccgggac ggaggcgtga aggtcgagct ggacgaagcg ctcccggacg
     6961 aggtccattc cgccgtccgg gccgccttgg agcgcgccct cgcccggctc ggcgccgccc
     7021 cggccattgc gacggtgcgg ggcataacgc cgcggttcga ccgcaagctg cgccgggtcg
     7081 agcgccaacg cgcctagagg tccatggtca gcgagcgctc ccgctccggc tcggcgagcc
     7141 cgttggccag ccgccagatg gtcgggccca tgacgtccag cggccccgtc gcgtagctcg
     7201 gcgcctcgcc ctggcgccac tcctgctgga ggcgcaggat ccgccggtcc tggttaagca
     7261 ccggcaggtg gaaagccttc aggacggcgc gcttcagcca ggccggagcg aggccccgcc
     7321 gggtcgcgaa gtgcgcccag gggcgggtca gcgtctcgtc ttccggcccg aacacgacgg
     7381 cgaccgagag cttgagctcg gcttcgccct cgaaggcgac ctcggcgatg gtcggcgggt
     7441 agaagcggcc gaggctcctc accctccttc cctcgagcag ccgcggcatc agtccggcgg
     7501 gcgtcgagcc ttcgaggtag gccgcctcgg cgccccacgg gccggagcgg accgacacct
     7561 ggaccggacg gcgccggttc ggcgagcgca ccatccagcg gtggacgtgg tgggggtggg
     7621 ccgggtcgag atgattctcg atcgcgtcga gcaggcccgc gggcgtcgct cggagcggcc
     7681 accagaagcg gtccagcgac tcgtcctcca tggcgcccgg aaggcggggg aacggatccg
     7741 gcgcctcggc caggctggtc cagaccagcc cggcccggtc cacgcagggc agagctttgg
     7801 ccggcgcccc cggaagcggc gagccgggca cggcgaggca ggcccctccc cgcccgaacc
     7861 gccagccgtg ataggggcag atgacggccc ccgcctccac gcgccctcgc gacagcggcg
     7921 ccccgcgatg cgggcagcgg tcgtcgagga cgaacgggcc gtcgccgtcg cgggcgacga
     7981 ccagccgccg ccccatcagc ctcgccgcga ccgggcgggc gccgaccgcc ggggagaagg
     8041 cgatggggtg ccaccccgag gcgatcgcgg cgggccacgc gcctaccatt gcttcgccac
     8101 catcaccggg acgaccaaac gcttcaggag catggcgagc gcttccaccg gcctgcgtct
     8161 cgccgccggg agatgcccgg cgtgataggc gttccattcg atgactcccc gcgccccgcg
     8221 cattcgcttg aaatgggccg ctccggccga gccgtgaagc cgccagccgg cctgcagagc
     8281 cctttgcatg aacatccagc aggcgatccg gtacaatccc tccctctgcg ggcgcgcggt
     8341 gtcgtagccg acgacagggg gagtcatcac ccctccccgc tcccacattc ccgccgccgc
     8401 ctggatggtg ccccgaggat cgcgggcgac ccggtaggac atcatcccgg accgggccga
     8461 ggcccggatg aaggccgggg tcaggatcgg attgaggggc gaatatttgc cgacgtagag
     8521 catggcgtag agctgtgcga tccgctcggc atcgtccgcc tcgacccgtg ccagttccga
     8581 gagggtcagc ggcgagcgct cgacgaggcg gcggtcgttg gcgaaatcgc tccgcttcgt
     8641 ccagtcgcgc ccgagatcgt cgacgaccca gatctgccgg ctcgggacca tgatccagcc
     8701 gtcggccgcc agcgcttcgc gaagccccgg cgcggaccag tcgtcgatcg agcgtatccc
     8761 gagcagatgg tcgggatgcg ccccggcgag cgcttcgcgc gcggccttga cgccttcgcc
     8821 gcgccagtcg ccgtggaggt tggtggagag gagccagttg ccgagatgga cgatccggtt
     8881 gatccgaccg gccttcagga ccaggtcgag ggccgcgagg gcggcgcgcg atggccaggc
     8941 ggtccagccg acccggagca ggtcgagctc ggcccgggcg tagaggacat aggcgctgtg
     9001 cgggctgcag acgtagcttc cgccaagggc gccgtcgtcg accgtgaccg gcatcgtccg
     9061 accggcgacg tccagcgtgg aaaaccgggt cgagacgttc gacaccatag agggggcgcc
     9121 gctttccacc atctcgcgaa gcatggcggc ggtcactcga aggcctcgcc attccattcg
     9181 atgtcgtcgg tggtctcgct ggtcgggccg tggaggcggg ccgcgcccat cagggcgaag
     9241 cgcgccgcgt cgagcaggac tccggcgacc gggagccggt cgcccggctt tccgacggcg
     9301 tcgcgcccct tgcgccagac cgcccgaagc cgcccgaagg tcccgtcggc gagtgcggct
     9361 gggacgccca taagggccat ggccggagag aggtagcgca tcgtccccgc gggcggcggc
     9421 ggcagctcgc cccggccaag catggccaag gcgagcgcgg gcgagccgtc catcaggtgg
     9481 accccgctca ccgcgcgcgg attgcattcg atcggcgcga tccggccgcc gggctggacg
     9541 atcacgtcga aggagagctg gccgtcgccg ccgacatgga cggcgatccg gcgcgccatt
     9601 tcgacgacct gaggctcatc caccgcctcg aaggcgaaag cggccgatcg gccgtgcctg
     9661 agggccggcc ggtagactat gcaggcgacg agcctgccgc cccgcaccgc cgaccagacg
     9721 caaagctcct ccccgtccag ccgttcctgg gcgacccagc gccggtccgc cgaggcgggc
     9781 agagccgccg ccatgccggc ttgcggcccg acgatcgtct tcgagccgaa gcgggagaat
     9841 tcgggcttga gaaccaggtc ggccggagcg agcggcacga ggttcgcgtc ggccgccgag
     9901 gcgatcgccc aggtcgccgg cgcctcgacg cccgcctcga gcgccagagc ggggaagctc
     9961 gccttggagt ggagcgcccg aagcgactcg atcgacggcg cgaacaggcg gtctgaccag
    10021 ccgtcgctcc gggcggcggc cgccagccag aacacctcct cgcaggtcgg gacgatgagc
    10081 tcatagcgtt cggccagggc gctcaggcgg gcccgaaagg ccgggaagtg ccggcgcggc
    10141 ggcggcagtc ggaggatctc gaaccgcggc ctcgaaagcg acgccgcgaa gggccgcacc
    10201 gaatccgcca gtgccacctc gcatccggcc gaccggaatg atcgcgccag gtccagagcg
    10261 accggcgccc gggcgccggt gatcatcacc cgggtcacct ttcctcctcg gccgagcgct
    10321 cggcgcagtg cgacggcgtg agcaggatgt cgggattgcg gactcgaagc gaatgcagct
    10381 tcgagagggt ttcgcggccg gacgccggat tgccgaggaa ggcggcggtg agagtcggcg
    10441 ccggccggtt ttcccggacc gcgcg
//
LOCUS       DASWNU010000039         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_468973, whole genome shotgun sequence.
ACCESSION   DASWNU010000039 DASWNU010000000
VERSION     DASWNU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468973"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VGB57_00835"
     CDS             <1..1436
                     /locus_tag="VGB57_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0129919.1"
                     /translation="IRGEKLRFEANPQLLQTTLDGLLELLAMADYATPHAFFETILSG
                     SLRGRHKLLERLGNEARDPIEELLSSALDFESASAPSLQRFLDWFARGEVEIVRDPSA
                     PLDAVRVMTVHGSKGLQSPVVILADACADPDRRGGGFGAAFANIPLTPEGPSIPIFRP
                     RKDELAEPLASQLAALDRREREEHWRLLYVALTRAEERLYIGGALGPADRKGPPPSSW
                     WTALDCALLGLGAEWQDDPHWGRARRFGDPESFTRAAPRQTVVEGLLPDWIRRPAPVE
                     ARPPRPLAPSALGEDDVADPPPSPAMRVAAQRGRLLHSLFERLPEVPREERRERAERW
                     LERSAGIDDPELRRGLVDDACRLIADPAHAELFGPGALAEAPIAAVVAGGVVVSGSVD
                     RLLVGPDRILVADFKTGRRAPETLDDVPVPHLRQMAAYVEALKIIFPGRPIKAKLLYT
                     AAPILFDLPESLLDRYRPVLEQASPAS"
     gene            1466..1780
                     /gene="trxA"
                     /locus_tag="VGB57_00840"
                     /pseudo
     CDS             1466..1780
                     /gene="trxA"
                     /locus_tag="VGB57_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            complement(1843..2286)
                     /locus_tag="VGB57_00845"
     CDS             complement(1843..2286)
                     /locus_tag="VGB57_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0129920.1"
                     /translation="MTGFLEGGCACGAVRYRLESEPFDCGWCHCRTCQRVSGSPAMVF
                     ASVPAGDLVWTRGADEVKSFKSSSFGHRSYCGSCGTPFLMEVDHQPETVDFSVSTLDD
                     PGKASPGFHIFWSSRLPWFEPKDELPRHARFRPDTRGLEGTEPPA"
     gene            2416..2691
                     /locus_tag="VGB57_00850"
     CDS             2416..2691
                     /locus_tag="VGB57_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129921.1"
                     /translation="MELLLFLSAMLAGLTGLISGDRAVEMRQVERTAVAASAAADFAA
                     QTAEASAHVSRPAVPAIPALAAASVPAFLATAPIVPQTAPVDERRLE"
     gene            2797..>3429
                     /locus_tag="VGB57_00855"
     CDS             2797..>3429
                     /locus_tag="VGB57_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY0129922.1"
                     /translation="MWGGVAKAIFGSSNDRYVRSIGKIVDKINGFEPTISAMSDDELA
                     NQTVLFRRRLAEGEELDALLPEAFATVREAAKRTLGQRHYDVQMIGGIVLHRGEIAEM
                     RTGEGKTLVATLAVYLNALEGKGVHVVTVNDYLARRDADWMGQVYRFLGLTVGVIVPN
                     LTDYQRRQAYGSDITYGTNNEFGFDYLRDNMKYERDALVQRPFNFAIVDEV"
BASE COUNT          549 a         1234 c         1083 g          563 t
ORIGIN      
        1 acattcgtgg cgagaagttg cgattcgagg cgaatcccca actcctgcaa accacgctcg
       61 acggccttct cgagctgctt gcgatggccg actatgcgac tccccacgcc tttttcgaga
      121 cgatcttgag cggaagcttg agaggtcgcc ataagctgct ggaaaggctc gggaacgagg
      181 cccgggaccc gatcgaggaa ctgctctcct cggccctgga tttcgaatcc gcctcggccc
      241 cttcgctgca gcgattcctg gactggttcg cccgcggcga agtggagatc gtccgcgatc
      301 cctcggcccc cctcgacgcg gtccgggtga tgaccgtcca cggctcgaaa ggcctgcaat
      361 ctccggtagt tatccttgcg gacgcctgcg ccgatccgga ccgccgcggt ggcggcttcg
      421 gagccgcctt cgccaacatc ccgctcaccc ccgagggccc gtccatcccc atcttccgtc
      481 cccgcaagga cgagctcgcc gaaccgctcg cctcgcagct cgccgccctc gaccggcgcg
      541 agcgggagga gcattggcgc ctcctctacg tcgctctgac ccgggccgag gagcgtctct
      601 acataggcgg cgccctgggg cccgcggacc ggaaggggcc gccgccgtcg agctggtgga
      661 ccgcgctcga ttgcgcgctg ctcggcctcg gcgccgagtg gcaggacgat ccccattggg
      721 gccgcgcccg ccgcttcggc gatcccgaat ccttcaccag ggcggctccc cgccagaccg
      781 tcgtggaagg gcttctgccg gactggatcc gtcgccccgc gcccgtcgaa gcccggccgc
      841 cgcggccgct cgccccctcg gctttgggcg aggacgacgt cgccgatccg ccgccctcgc
      901 cggcgatgcg ggtggcggcc cagcgcgggc ggcttctcca ttccctgttc gagcggctcc
      961 ccgaggttcc gcgggaggag cgccgggagc gcgccgagcg ctggctcgag cgttccgccg
     1021 ggatcgacga tccggagctc cgccgaggcc ttgtcgacga cgcctgccga ctcatcgcgg
     1081 atccggccca tgccgagctg ttcggccccg gcgccctcgc cgaggcgccg atcgccgcgg
     1141 tggtcgccgg aggcgtggtc gtctcgggct cggtcgaccg gctgctcgtc ggaccggacc
     1201 ggatcctcgt cgccgacttc aagaccggcc gccgcgcccc ggagacgctc gacgacgtcc
     1261 cggtcccgca cctccgccag atggccgcct atgtcgaggc tctgaaaatc atcttcccgg
     1321 gccgtcccat caaggcgaaa ttgctctaca cggcggcgcc catcctgttc gacctgccgg
     1381 aaagcctgct cgaccgctac cggccggtgc ttgagcaggc gtctccggct tcctaaatgg
     1441 cattcgaccc cttcaggaga ttcccatgac caaatccgtc agcgacgaca ccttccagaa
     1501 cgaggtgctc gcttccgaca agcccgttct cgtcgatttc tgggcggaat ggtgcggtcc
     1561 ctgccggatg atcgctcccg cgctcgagga gatcagccag gagatggccg accgggtcac
     1621 cgtggtgaag ctcaatatcg acgaaaatcc gacggccccg gcgaaatacg gcgtccgcgg
     1681 catcccgacc atgatattgt tcgaggacgg caagatcgcc gcgaccaagg tcggcgccgc
     1741 tccgaagact cagctgcaga gctggctcga gcaggtgctg aacggcctcg actacaagaa
     1801 acagcacagc gacagcgaca gcggcggcgc cgacgagttc aactaagctg ggggctcagt
     1861 cccttcgagc ccgcgcgtgt cgggccggaa cctggcatgc ctcggcagct cgtccttcgg
     1921 ctcgaaccag ggcagccggc tgctccagaa gatgtggaag ccggggctcg cctttccggg
     1981 atcgtcgagg gtcgagacgc tgaaatcgac cgtctcgggc tgatggtcga cctccatcag
     2041 gaacggagtg ccgcatgagc cgcaatagct gcggtggccg aagctcgacg acttgaacga
     2101 cttcacctcg tccgcgcccc tggtccagac gaggtcgccc gccggaacgc tcgcgaacac
     2161 catcgccggg gagcccgaga cgcgctggca ggtgcggcag tggcaccagc cgcaatcgaa
     2221 gggctcgctt tcgagccggt agcggaccgc gccgcaggcg cagcccccct cgagaaatcc
     2281 ggtcatggcg acggccgcta ggcttcagcc ggccgccgcg tcaatccttg gcctccaccc
     2341 atggactgcg gcggcctcaa tcactatgtc tcgacggcgc ggcgctcgaa gtgggcgcct
     2401 cacctggaac tggctttgga acttctgctt ttcctctcgg cgatgctcgc cggcctcacc
     2461 ggcctgatct cgggcgaccg ggccgtcgag atgcgccagg tcgagcgcac cgccgtcgcc
     2521 gcgagcgcgg ccgccgattt cgccgcccag accgcggaag cctcggctca tgtctcgaga
     2581 ccggcggttc ccgccattcc cgcccttgcc gccgcgtcgg tgcccgcatt ccttgcgacc
     2641 gcgccgatcg tgccccagac cgctcccgtc gacgagcgcc ggctggaata gcaggcccga
     2701 ccccgcctcg ccgcgggtct ttatcttcga taagcgccca ccggcgccgc gcgccctcgg
     2761 cgcattcgca atattcagac aagacaggat ttcgatatgt ggggcggagt cgccaaagct
     2821 attttcggat cgtcgaacga ccgatacgtc cgctcgatcg gcaagatcgt cgacaagatc
     2881 aacggcttcg agccgaccat ttcggcgatg tcggacgacg agctcgccaa ccagaccgtc
     2941 ctgttcaggc ggcgtcttgc cgagggcgag gagctcgatg cccttcttcc cgaggcgttc
     3001 gcgaccgtcc gcgaggctgc aaagcggact ctcggccagc gccattacga cgtccagatg
     3061 atcggcggga tcgttctcca ccgcggcgag atcgccgaga tgcggaccgg cgagggcaag
     3121 accctggtcg cgacgctggc tgtctacctc aacgcactgg aagggaaggg agtccacgtc
     3181 gtcaccgtga acgactatct cgcccgccgc gacgccgact ggatgggcca ggtctaccgc
     3241 ttcctcgggc tgaccgtcgg ggtcatcgtc cccaatctca ccgactatca gcggcgccag
     3301 gcctatggtt cggacatcac ctacggcacc aacaacgaat tcggattcga ttatctgcgc
     3361 gacaacatga agtatgagcg cgacgccttg gtccagcgcc ccttcaattt cgcgatcgtc
     3421 gacgaggtt
//
LOCUS       DASWNU010000040         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_79160, whole genome shotgun sequence.
ACCESSION   DASWNU010000040 DASWNU010000000
VERSION     DASWNU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79160"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..697)
                     /locus_tag="VGB57_00860"
     CDS             complement(47..697)
                     /locus_tag="VGB57_00860"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY0129923.1"
                     /translation="MTAIEELKRRLGECPLVAILRGVSHREVEAIGQALFDAGIRIIE
                     VPLNSPNPLASIQLLARRFGEEALIGAGTVTDVTEVALVRGAGARVIVSPNTFTPVIE
                     AAAAAGLVSLPGFFTPSEAFAALRAGATGLKLFPAEGATPAVVKAQRAVLPKDVPLLV
                     VGGISPGNMQPWLDAGAQGFGLGSGLYKPGQSAEETGDRARAYIKGLRGISPLEGT"
     gene            complement(694..1581)
                     /locus_tag="VGB57_00865"
     CDS             complement(694..1581)
                     /locus_tag="VGB57_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY0129924.1"
                     /translation="MRWAEGFIAVDWGTTNRRAWRLDSGGAVAGEMEDDQGILSVAEG
                     GFHSAVERIRARLGDLPMLMAGMVGSNRGWMEAPYVPCPAGLPEVASRLLWIEGERAA
                     IVPGVSYDLDGAADVMRGEEVQLLGAYAEGWIGPDSILCHPGTHNKWVRLEDGRIAAF
                     RTIMTGELFNLLKAHSILADLLAEPAEPGPAFEAGVRHGLDRDDLTAELFSVRARTLL
                     GKAPREEASAYVSGLLIGADLKVGLRLAGESEVVTIGRPELTRLFMAALESTGRSSRA
                     IDGEAAFLAGIRHLVELIR"
     gene            1665..2540
                     /locus_tag="VGB57_00870"
     CDS             1665..2540
                     /locus_tag="VGB57_00870"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0129925.1"
                     /translation="MIAESTVECVADVHAVLGEGPVWAARESALYWVDIKGRKIFRID
                     AEGGRREWTTPYRVGSLAPRAGGGFIGGTDQGIAEIDLDAGRFDILHNPEEHLPDNRF
                     NDGKVDRSGRFWAGTMDDTEKDSTGTLYRIAGDLTATAIDAGYKVTNGPAFSPAGDTM
                     YHNDSARQVTYAFDLDADGKASNRRTFATYADGDGYPDGMTIDTEGCLWIAFWDGWCV
                     RRYSPSGECIAKLDVPVSKPTSCAFGGPALDQLYITSASIGLDERDLAGQPQAGGLFV
                     AAPGVTGLAELPFAG"
     gene            2590..>3143
                     /locus_tag="VGB57_00875"
     CDS             2590..>3143
                     /locus_tag="VGB57_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0129926.1"
                     /translation="MFATGIENSIPTINHGKTRIDQMEVGAHYERWREDFDCVQEIGI
                     NFLRYGAPLHRTWLGPNRYDWEFADLTFADLKRREITPILDLCHFGVPDWVGNFQNPD
                     FPQLFAAYAGAVADRYPWIQLYTPINEMFICAVFSAKYGWWNEQATDDRSFFSAIKNI
                     VKANVIAMIEILKRRPDAIFVQSE"
BASE COUNT          558 a         1086 c          995 g          504 t
ORIGIN      
        1 cgcgcggtcc ccctcccccg gaaatccggg ggaggactta gaagccctag gtcccctcca
       61 gtggcgaaat cccacgaagc cccttgatat aagcccgcgc cctgtccccg gtttcctcgg
      121 ccgactggcc cggcttgtag agtcccgaac ccagcccgaa tccctgcgcc cccgcgtcga
      181 gccagggctg catgtttccg gggctgattc cgccgacgac gagcaatggc acgtccttcg
      241 gaagcaccgc acgctgcgcc ttcaccaccg ccggggttgc gccctcggcc ggaaacagct
      301 tcagacccgt cgcccccgct ctcaaggccg caaaggcctc cgacggcgtg aagaagccgg
      361 ggagcgacac cagtcccgcc gccgcggcgg cctcgatcac cggagtgaac gtgttgggcg
      421 agacgatcac ccgtgcgccc gcgcccctca ccagcgccac ctcggtgacg tccgtcacgg
      481 taccggcgcc gatcagtgcc tcctcgccga agcgccgggc gaggagctgg atgctggcga
      541 ggggattggg cgagttgagc ggcacctcga tgatccggat cccggcatcg aagagcgcct
      601 ggccgatcgc ctccacctcg cggtgagaca ctccccggag gatcgcgacc agcgggcatt
      661 caccgagcct gcgcttcagt tcttctatag ccgtcatcga atgagctcca ccagatgcct
      721 gattccggcc aggaaggccg cttccccgtc gatcgcccgc gaggaccgcc cggtcgattc
      781 gagggcggcc atgaaaaggc gggtaagctc cggccgcccg atcgtcacca cttcgctttc
      841 tccggcgagg cgcaagccga ccttcagatc ggcgccgatc agaagcccgc tgacataggc
      901 gctcgcctct tcgcgcggcg ccttgccgag cagggtgcgg gcgcggaccg agaacaattc
      961 ggcggtgagg tcgtcgcggt cgaggccgtg ccggacgccc gcctcgaagg ccgggccggg
     1021 ctccgcgggc tcggcgagca gatcggcgag gatgctgtgc gccttgagga gattgaacag
     1081 ctcgccggtc atgatcgtcc ggaaggcggc gatccgcccg tcctcgagcc ggacccattt
     1141 attgtgggtg cccgggtggc acaggatcga gtcggggccg atccaaccct cggcataggc
     1201 gccgagcagc tggacctcct cgccgcgcat cacgtccgcc gctccgtcca aatcgtagga
     1261 tactccaggc acgatggccg ctcgctcgcc ctcgatccag agcagccgcg acgcgacctc
     1321 gggcagcccg gcggggcagg ggacataggg cgcctccatc cagccgcgat tggagccgac
     1381 catcccagcc atcagcatcg gcaggtcgcc gagccgggcc cgtatccgct cgacggccga
     1441 gtggaaaccg ccctccgcca ccgagagaat gccctgatcg tcctccatct cgcccgcgac
     1501 ggcgccgccc gaatccagcc gccaggcacg ccggttggtc gtgccccagt cgacggcgat
     1561 gaaaccttct gcccagcgca tgctttgacc cattagcgaa aaacattggc cgcgcgacat
     1621 gggtccgcgg ttttcttttg agcggcggga gctatcgggg cgccatgatt gccgaatcga
     1681 ccgtcgaatg cgtcgccgat gtccatgccg tgctcggcga agggccggtg tgggccgcgc
     1741 gcgagtccgc gctctattgg gtcgatatca agggccggaa gattttccgc atcgacgccg
     1801 aaggcggacg gcgggagtgg acgactccgt atcgcgtcgg gtcgctggcg ccgcgcgcgg
     1861 gcggggggtt catcggcggg accgaccagg ggatcgccga gatcgacctc gacgccgggc
     1921 gcttcgacat ccttcacaac ccggaagagc atctgccaga caaccgcttc aacgacggca
     1981 aggtcgatcg cagcgggcgc ttttgggcgg gaacgatgga cgacaccgaa aaagattcga
     2041 cggggacgct gtaccgcatt gcgggcgacc tcaccgcgac ggccatcgac gcgggttaca
     2101 aggtgaccaa cggccccgca ttcagcccgg ccggcgacac catgtatcac aatgattcgg
     2161 cgcggcaggt gacctatgcg ttcgatctcg acgccgacgg caaggccagc aaccgccgca
     2221 cctttgccac ttacgccgac ggcgacggat atccggacgg catgaccatc gataccgaag
     2281 gctgcttgtg gatcgccttc tgggacggct ggtgcgtgcg ccgttattcc ccctccggcg
     2341 agtgcatcgc caagctagac gtgccggttt ccaagccgac cagctgcgcg ttcggcgggc
     2401 ccgccctcga ccaattatac atcacttccg cgagtatcgg cctcgacgag cgggatctcg
     2461 ccggccaacc gcaagcaggg ggtttgtttg tcgcggcgcc cggcgttact ggtctagcgg
     2521 agcttccctt tgcaggctga ccaggccgaa ccaggcagcg gtcgaggaga atgagccatc
     2581 gctaacttca tgttcgcgac cgggatcgaa aacagcatcc cgacgatcaa tcacggcaag
     2641 acccgcatcg accagatgga agtgggcgcc cattacgagc gctggcgcga ggacttcgac
     2701 tgcgtccagg aaatcggcat caatttcctg cgctacggcg cgccgctgca ccgcacctgg
     2761 ctgggcccca acagatatga ctgggagttc gcggacctca ccttcgccga tctgaagcgc
     2821 cgcgagatca cgccgatcct cgacctctgc cacttcggcg ttcccgattg ggtcggcaat
     2881 ttccagaacc cggatttccc gcaattgttc gccgcttatg ccggcgcggt cgccgaccgc
     2941 tatccgtgga tccagctcta cacgccgatc aacgagatgt tcatttgcgc ggtcttctcc
     3001 gcaaaatacg gctggtggaa cgagcaggcg acggacgacc gcagcttctt ctccgcgatc
     3061 aagaacatcg tcaaggcgaa cgtcatcgca atgatcgaga tcctgaagcg ccggccggac
     3121 gcgatcttcg tgcaatcgga aag
//
LOCUS       DASWNU010000041         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_209361, whole genome shotgun sequence.
ACCESSION   DASWNU010000041 DASWNU010000000
VERSION     DASWNU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209361"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1657)
                     /locus_tag="VGB57_00880"
     CDS             complement(170..1657)
                     /locus_tag="VGB57_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0129927.1"
                     /translation="MAVRLLSALALLLLALPASAEPVSTEALRRHIDVLASDGFAGRE
                     PGTEGERKSIAYIAGQMRAFGLEPAGPGNSWYQPVEVKGRRPGDQTSVWQARGGKGER
                     VELGRDEIILLGRSARETLAQAPVVFAGHGAVIADKGIDQLAGADLKGAVVLILYDTP
                     EGAGLPGYSERAAAVAAAGAAAVIGIMDQELPWPVVQRVYDAGQKRLAIHDPARLAGA
                     ISQAAAESVLRAGGADLAALLATPGPGFKAVPLKLRASFDVATAIETIQTHNVVGRIR
                     GSGGGKESLLYLSHWDHLGLCDREGPDRICNGAVDNASGIAALLEIARALGRGKRPKR
                     DILFLATTAEEMGLLGAEYFASRPVVPLGSIVAAINMDTLAITPRGEPVAVMGRGRAA
                     LDSVIDRTIAEAGRTPDRDDEAAAFVERQDGWALARAGVPAIMVGGSFADMEKLGAFL
                     GGPYHSAADNPGPGLMLEGAAEDTDLMIALGRKLTDPALYPWLGQ"
     gene            complement(1773..2531)
                     /locus_tag="VGB57_00885"
     CDS             complement(1773..2531)
                     /locus_tag="VGB57_00885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEY0129928.1"
                     /translation="MENRTEPPRPTEGGEMTSTPSPERLRDLTINLVRSIGPNWHRHS
                     LVAMRVEALARLLYYNNLYQMIVDVPGVICEFGVQWGATMTELINLRNIYEPFNVSRT
                     IIGFDTFEGFPSVHAMDGGHSSVGDYNSMPGYEEQLEEILALHESFGALSSPRRFSLV
                     KGDVSVTAPAWLEENPHAIVSMAIFDLDLYQPTKDALEAIRPRLVKGSLLVFDELNCA
                     RFPGETRALDEVMGLGNLTLRRSPLQPFCAWAVV"
     gene            complement(2633..3433)
                     /locus_tag="VGB57_00890"
     CDS             complement(2633..3433)
                     /locus_tag="VGB57_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY0129929.1"
                     /translation="MIADLKLCRSLLFLPASNPRAIEKARGLGADIVVLDLEDAVKPA
                     DKDEARAAAVAAAAEGFPGLCAIRINSGAPWHEADLEAAAASRADYVIVPMTPGPEAP
                     REAARATGKPVLAMIETARGVLNAAAIAPETVGLVAGTNDLAADLGLRPGGGRAPLVT
                     ALQTILRAARAAGVAAFDGVYNRLDDAQGFAAQCEEGKSFGFDGKSLIHPSQIEPANR
                     LFGPSEAEIEAAERLLAAATGGAERFEGEMIETMHVEQARRVLARARA"
     gene            3507..4511
                     /locus_tag="VGB57_00895"
     CDS             3507..4511
                     /locus_tag="VGB57_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0129930.1"
                     /translation="MTISIVFFFLLILVVLFLMSGIKIVRQGYHYTIEYFGRFTAVAH
                     PGFNFYMPFFYRVGRRVNMMEQVLDIPGQEIITKDNAMVSTDGVVFFQVLDAAKAAYE
                     VSDLYVAIMQLTTTNLRTVMGSMDLDETLSKRDEINARLLSVVDQATESWGVKITRVE
                     IKDIRPPADIVNAMGRQMKAEREKRAVILEAEGSRASAILRAEGEKQGQILEAEGRRE
                     AAFRDAEARERAAEAEAKATQVVSDAIAGGNTQAINYFIAQRYVDAIGGFATSPNAKT
                     ILFPVEATALIGTLGGIGELARDALGGGGGGDGGAGAGGGAPARRRGPFEGRGEVPGQ
                     "
     gene            4538..>4942
                     /locus_tag="VGB57_00900"
     CDS             4538..>4942
                     /locus_tag="VGB57_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY0129931.1"
                     /translation="MWMLGAILLGIAELIVPGVFLIWLAAAAAATGLLTLAFGIELPF
                     QFALFALLAIAAVYFGRRWYVNNPMPSADPLLNDRAARLTGRTVLVTAAIVNGEGRVR
                     VGDSVWSCRGPDCSEGSRVRITGADGSCLKVEP"
BASE COUNT          791 a         1706 c         1669 g          776 t
ORIGIN      
        1 atgcgtttca tattcgatta cccccggatt ccccacccgc ttccatagca gaatcgccgg
       61 cgcggcgcga atgaccgggg cgccgcgcct tccagctgct gcatgcgcgc tatcccaatt
      121 ccgcccgggc gaaacgctcc ccttattatt acgacgagtg agcccgagcc tattggccca
      181 gccaggggta gagcgccggg tccgtcagct tgcggccgag ggcgatcatc aggtccgtat
      241 cctcggcggc gccttcaagc atcaggcccg gacccggatt gtcggccgcc gaatgatagg
      301 gcccgccgag aaaggcgccg agcttctcca tgtcggcgaa ggagccgccc accatgatcg
      361 cgggcacacc ggcccgggca agggcccagc cgtcctggcg ctcgacgaag gcggcggcct
      421 cgtcgtcgcg gtcgggggtc cggcccgctt cggcgatcgt ccggtcgatg actgagtcga
      481 gcgcggcccg accccggccc atcacggcca ccggctcgcc cctgggcgtg atcgccagtg
      541 tgtccatgtt gatcgccgcg acgatcgagc cgagcggaac cacggggcgc gaagcgaaat
      601 attcggcgcc gagcaggccc atctcctccg ccgtggtggc gaggaagagg atgtcgcgct
      661 tcggccgctt cccccggccc agtgcccggg cgatctccag caaggcggcg atcccgctcg
      721 cattgtcgac cgcgccgttg cagattcggt ccggaccttc cctgtcgcag aggccgagat
      781 ggtcccaatg ggaaagatag agcaagctct ccttgccgcc gccggagccg cggatccgcc
      841 cgaccacgtt gtgggtctgg atcgtctcga tcgcggtggc gacgtcgaag ctggcgcgca
      901 gcttgagcgg caccgccttg aagcctggtc ccggcgtcgc gagcagggcc gcgaggtcgg
      961 cgccgccggc gcgcaggacg ctttcggccg cggcctgcga gatagcaccc gccagccggg
     1021 cgggatcgtg gatggcgagc cttttctggc cggcgtcgta gacgcgctgg accaccggcc
     1081 agggcaactc ctggtccatg atcccgatca ccgccgcggc gcccgcggcc gcgaccgccg
     1141 cggcccgctc ggaatagccc gggaggcccg ccccctccgg cgtgtcgtag agaatgagca
     1201 cgaccgcgcc cttgaggtcg gccccggcga gctggtcgat gcccttgtcg gcgatcaccg
     1261 cgccgtggcc ggcgaagacc accggcgcct gcgccagggt ctcccgagcc gagcggccga
     1321 ggaggatgat ctcgtcgcgc ccgagctcga cccgctcgcc tttgccccct cgcgcctgcc
     1381 agacgctggt ctggtcgccc ggacggcggc ccttcacctc gacgggctgg taccagctgt
     1441 tgccaggccc cgccggctcg agcccgaaag cccgcatctg cccggcaatg taggcgatcg
     1501 atttgcgctc gccctccgtc ccgggctcgc ggcctgcgaa gccgtcgctg gcgagcacat
     1561 cgatatggcg gcgaagcgcc tcggtcgaga cgggctcggc cgaggcggga agggcaagga
     1621 ggaggagcgc gagagcggag agcaagcgta cggccatccc ctcgcttaag tcgacggagg
     1681 ctcgagtggc aagaggaggc ttcgcactga cgccccggca gggagagtca ggggagactg
     1741 ggcgcgcagc ccgtcctgcg gcggagtgcc ccttagacca cggcccatgc acagaaaggc
     1801 tggagcgggg agcgacgaag ggtgaggttg ccaagaccca tcacctcgtc caacgcccgc
     1861 gtttctccgg gaaaacgagc gcaattcagc tcgtcgaaca cgagcagcga ccccttcacc
     1921 aggcgaggac gtatggcttc cagagcatct ttcgtcggct ggtaaagatc caggtcgaag
     1981 atcgccatgg agacaatggc gtgcggattc tcctctagcc atgccggcgc cgtcaccgac
     2041 acgtctccct tgaccagcga aaatcggcga ggcgacgaca gagcgccaaa ggattcgtgg
     2101 agggcaagta tctcttccag ttgctcctca tagcctggca tggaattgta atccccgacc
     2161 gaggaatgtc ccccgtccat ggcatgcacg gatgggaaac cctcaaaggt atcgaatccg
     2221 attatcgtgc ggctgacgtt gaacggttcg tagatgttcc ggaggttgat aagttcggtc
     2281 atcgtggctc cccactgaac gccgaactca caaatcactc caggcacgtc gacgatcatc
     2341 tgatacaaat tgttgtaata cagaagccga gcgagggctt cgacgcgcat cgctaccagg
     2401 ctgtgccgat gccagttcgg accgatactg cggaccagat tgatcgtgag gtcacgaagt
     2461 cgctccggcg atggggttga cgtcatctcg ccaccctctg tggggcgagg cggttcggta
     2521 cgattttcca tggcgtcgcc aaagccgaag ggagggagag gatcaagcct tttttaggct
     2581 cgccctctcc gggccgcggt cacccgctgc ggccttgctg cgaggggagg attcaagccc
     2641 gggccctggc aagcacccgg cgcgcctgct cgacgtgcat ggtctcgatc atctcgccct
     2701 cgaagcgctc cgctccgccg gtcgctgccg cgagcaggcg ctcggccgcc tcgatctccg
     2761 cctcgctcgg accgaagagg cggttcgccg gctcgatctg gctgggatgg atgagcgact
     2821 tgccgtcgaa gccgaagctc ttcccctcct cgcactgggc ggcgaagccc tgcgcatcgt
     2881 cgagccggtt atagactccg tcgaaggcgg ccacccccgc cgcgcgcgcg gcccggagga
     2941 tggtctggag tgcggtgacc aaaggggccc ggccgccgcc ggggcgaagg ccgaggtccg
     3001 cggcgaggtc gttggtgccg gcgacgaggc ccaccgtctc cggagcgatg gcggcggcgt
     3061 tcaggactcc gcgggcggtc tcgatcatgg cgaggacggg cttgccggtg gcccgggccg
     3121 cctcgcgggg cgcttcgggc ccgggagtca tcggcacgat cacatagtcg gcgcggctgg
     3181 ccgcggcggc ctcgaggtcc gcctcatgcc agggcgcgcc ggaattgatc cggatcgcgc
     3241 acaggccggg gaacccctcg gccgccgccg cgaccgccgc ggcgcgcgcc tcgtccttgt
     3301 ccgcgggctt caccgcgtcc tcgagatcga ggacgacgat gtccgcgccg agccccctcg
     3361 ccttctcgat cgcgcgcggg ttcgaggcag gcaggaagag cagtgaacgg cagagcttga
     3421 ggtcggcgat catgtcccga tttcgcttgg aagaggtccc acttgcccgc attatgccgc
     3481 cgttcgcaag gggaggcgcg tcggtcatga ccatcagcat cgttttcttc tttctgctta
     3541 tcctggtcgt gctgttcctc atgtccggca tcaagatcgt ccgccaaggc tatcattaca
     3601 cgatagaata tttcggccgc ttcaccgccg tcgcccatcc cggcttcaat ttctacatgc
     3661 ccttcttcta tcgcgtcggg cgccgcgtga acatgatgga gcaggtgctc gacatccccg
     3721 ggcaggagat catcaccaag gacaacgcca tggtctcgac cgacggagtc gtcttcttcc
     3781 aggtgctcga cgccgccaag gccgcctatg aggtctccga cctctacgtc gcgatcatgc
     3841 agctcaccac caccaacctg aggacggtga tgggctcgat ggacctcgac gagaccctgt
     3901 ccaagcgcga cgagatcaac gcgcggctgc tcagcgtggt cgaccaggcg accgaaagct
     3961 ggggcgtcaa gatcacccgg gtcgagatca aggacatccg gccgcccgcc gacatcgtca
     4021 acgccatggg ccgccagatg aaggccgagc gcgagaagcg cgccgtcatc ctcgaggccg
     4081 agggcagccg cgcctccgcc atcctccgcg ccgagggcga gaagcagggc cagatcctcg
     4141 aggccgaagg ccgccgcgag gccgccttcc gcgacgccga ggcgagggag cgcgccgccg
     4201 aggccgaggc caaggcgacc caggtcgtct ccgacgccat cgccggcggc aacacccagg
     4261 cgatcaacta tttcatcgcc cagcgctatg tcgacgcgat cggcggcttc gccacatcgc
     4321 ccaacgccaa gaccatcctc ttcccggtcg aggcgacggc gctcatcggc accctgggcg
     4381 ggatcggcga gcttgccagg gacgccctcg gcggcggcgg cggcggcgat gggggcgcgg
     4441 gtgcgggcgg aggcgcaccg gcccggcggc gcgggccgtt cgagggccga ggcgaggtcc
     4501 ccggccagtg agcggcctgg agctcgagcc ccattggatg tggatgctgg gggcgatcct
     4561 gctcggcatc gccgagctga tcgtgccggg cgtcttcctg atctggctcg ccgccgcggc
     4621 ggcggcgacc ggcctcctca ccctcgcctt cgggatcgag ctgccgttcc agttcgccct
     4681 cttcgccctc ctcgccatcg ccgccgtcta tttcggccgg cgatggtacg tgaacaaccc
     4741 gatgccgagc gccgatcccc tgctcaacga ccgagccgcc cgcctcaccg gccggacggt
     4801 cctggtcacc gccgcgatcg tcaacggcga gggccgggta agggtcggag acagcgtctg
     4861 gtcctgccgg gggccggact gctcggaggg gtcgcgggtt cggatcacgg gagcggacgg
     4921 gtcctgcctc aaggtagagc cg
//
LOCUS       DASWNU010000042         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_105342, whole genome shotgun sequence.
ACCESSION   DASWNU010000042 DASWNU010000000
VERSION     DASWNU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105342"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /gene="ppnK"
                     /locus_tag="VGB57_00905"
     CDS             <1..92
                     /gene="ppnK"
                     /locus_tag="VGB57_00905"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952362.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEY0129932.1"
                     /translation="RVDRSLALTLLFDPEHALDERITMEQFAV"
     gene            146..220
                     /locus_tag="VGB57_00910"
     tRNA            146..220
                     /locus_tag="VGB57_00910"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:178..180,aa:Asn,seq:gtt)
     gene            271..1032
                     /locus_tag="VGB57_00915"
     CDS             271..1032
                     /locus_tag="VGB57_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129933.1"
                     /translation="MPNVLILTPIKDAAVHLPGYFANILALTFPPEQLSIGLLEGDSR
                     DGTQSVAEAGIAMLAKRLRRAGLWRRDFGFRMTPGLPRWAAQHQLQRRTILAKSRNHL
                     LSRALQDEDWVLWLDVDVVEYPPDLIETLIGFGRDIVHPNCVRTYGGPSFDHNAWVGP
                     GELHLDQLRGGEKLVRLDSVGGTALLVRADLHRDGLVFPPFLYGRPHPTVRRPGPWRP
                     LEPGEIETEGLAMMARDMGVDCWGVPDLEVRHSPH"
     gene            complement(1045..1596)
                     /locus_tag="VGB57_00920"
     CDS             complement(1045..1596)
                     /locus_tag="VGB57_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129934.1"
                     /translation="MTDRRLSIAGSMTPIDPAAVVGWGVDADTENDPTYPYRDRSADE
                     GLTSDWARPPLQRSDVEILQSVEHIRRPAVFGTSTPPSGLSGVIRRGAFRFTESNWMH
                     WLMLMGADRINVVEGVLQDLGRGKIPNIPGEMGIRSELRYNKKGFAKKVAVTAVVSTA
                     AWALLRRRRRNRERALEGAGTDA"
BASE COUNT          253 a          578 c          553 g          291 t
ORIGIN      
        1 tccgtgtcga ccgaagcctc gccttgaccc tgctcttcga tccggagcac gcgctcgacg
       61 agcggattac catggagcaa ttcgccgtct gaggcttgca gtccggaaaa acatgctgct
      121 ataggcgccc tcgcctcatg gctgctcccc ggtagctcag cggtagagca tccgactgtt
      181 aatcggatgg ccgatggttc gaatccgtcc cggggagcca ctctcgacgt ctccgtccgg
      241 cgtcggcccg gcccagggga cccgaactcc atgcccaatg tgctcatcct gacgccgatc
      301 aaggacgcgg cggttcacct gccgggctat ttcgccaaca tcctcgccct gaccttcccg
      361 cccgagcagc tttcgatcgg gctcctcgaa ggcgacagcc gcgacgggac ccaaagcgtc
      421 gcggaagccg gaatcgcgat gctggccaag cggttgcggc gggccggcct gtggcgcagg
      481 gatttcggct tccgaatgac accgggcctg ccgcgctggg cggcgcagca ccagctccag
      541 cggcgcacca tcctcgccaa atcgcgcaac cacctattgt cccgcgcgct ccaggacgag
      601 gattgggtgc tctggctcga cgtcgacgtg gtcgaatatc cgccggacct gatcgagacg
      661 ctgatcggct tcggccgcga catcgtccat cccaactgcg tcaggaccta tggcggcccg
      721 tctttcgatc acaatgcctg ggtcggaccc ggtgagctgc acctcgacca gctccgcggc
      781 ggcgagaagc tggtgcggct cgattcggtc gggggcaccg cgctgctggt ccgcgccgac
      841 ctccatcgcg acgggctcgt cttcccgcct ttcctctacg gccgtccgca tcccacggtg
      901 cggcggccgg ggccctggcg gccgctcgag cccggcgaga ttgagacgga gggcctcgcc
      961 atgatggcgc gcgacatggg cgtcgattgc tggggcgtcc ccgatctcga ggtgcgccac
     1021 tcgccgcact gagtcggaag gggctcaggc gtcggtgccg gccccttcca gtgcgcgctc
     1081 ccggtttcgc ctgcgtcgcc gcagcaatgc ccaggccgcc gtcgatacga cagccgtgac
     1141 ggcaaccttc ttcgcgaagc ccttcttgtt gtacctgagc tccgagcgga tccccatctc
     1201 gccgggtatg ttcggaatct tgccgcggcc gaggtcctga agcacgcctt cgacgacgtt
     1261 gatccggtcc gcgcccatca gcatcagcca atgcatccag ttcgattcgg tgaagcggaa
     1321 ggcgccgcgg cggatgacgc cgctgagccc gctcggcgga gtcgaagtcc cgaacacggc
     1381 cggccgccgg atatgctcga ccgactggag gatctcgacg tcggagcgct ggaggggcgg
     1441 ccgcgcccag tcgctggtga ggccctcgtc ggcggaccgg tcgcgatagg gataggtggg
     1501 atcgttctcg gtgtcggcgt cgacgcccca tcccacgacc gcggccggat cgatgggggt
     1561 catgctgccg gcgatcgaga ggcggcggtc ggtcatgatc gtgtcctcaa ccattgggca
     1621 ccagcacggg cttgatgcag ccgtcgagct tggcggagaa gatgtggtag ccctc
//
LOCUS       DASWNU010000043         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_390409, whole genome shotgun sequence.
ACCESSION   DASWNU010000043 DASWNU010000000
VERSION     DASWNU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390409"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VGB57_00925"
     CDS             <1..527
                     /locus_tag="VGB57_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY0129935.1"
                     /translation="LLRGPVRADARGWFHKTFHVEAFADAGLRTDWREEYCSLSRRSV
                     LRGMHFQVPPADHAKLVYCLAGEVLDVVVDLRAGSPTFGEHRAFTLTPEAGAGLYIPE
                     GLAHGFLGLSAESLMYYKVTSVHAPDADRGIAWDSFGFDWPVDAPLLSDRDRGHPPLA
                     EFHSPFRFGPAESR"
     gene            524..1417
                     /locus_tag="VGB57_00930"
     CDS             524..1417
                     /locus_tag="VGB57_00930"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0129936.1"
                     /translation="MSRTALLTGVTGFIGGTLARRLLAEGWRVHAIVRPGSDVGRVTP
                     HERLALHAHDGGTESLIRLVQTAAPDVAFHLASLFLADHRPDQIEALVASNLLFPVQL
                     AEALTRTGVLRLVNTGTAWQHRHSEPYLPVNLYAATKQAFEDLLLYHHDACGLSVVTL
                     KLFDTYGAGDPRRKLIGILLEAARTGEALPMSPGEQVLDLTHVEDAVEGYLIAAERLL
                     ASKEPLRETYFLPGERHRLRDLVRLVERAVGRPLDVNFGGRAYRPREVMNPIEAESDR
                     LLPGWTRRRDLLTTLPELLAP"
     gene            1414..>1658
                     /locus_tag="VGB57_00935"
     CDS             1414..>1658
                     /locus_tag="VGB57_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129937.1"
                     /translation="MTGSPGILRRHRAVAIGLVAQLLQYGGALLMLPLVLTRLSAAEV
                     AIFYVFLTIQGLSLLADLGFQPTFARAFASAFAGASEL"
BASE COUNT          221 a          615 c          527 g          295 t
ORIGIN      
        1 atctgcttcg cgggccggtt cgggccgatg cacgcggctg gttccacaag acgttccacg
       61 tcgaggcatt cgcggacgcg ggcctgcgga ccgactggcg cgaggaatat tgctccctct
      121 cccgccgctc cgtgcttcgc gggatgcact tccaggtccc gccggccgac cacgccaagc
      181 tggtctactg cctggcaggc gaggtgctcg acgtcgtcgt cgaccttcgc gccggatcgc
      241 cgacgttcgg ggaacaccgc gccttcacgt tgaccccgga ggccggcgcc ggcctgtaca
      301 tccccgaggg cctggcccat ggcttcctcg ggctcagcgc ggaaagcctg atgtattaca
      361 aggtcaccag cgtccacgcg cctgacgctg atcgcggcat tgcctgggat tcgttcggtt
      421 tcgattggcc ggttgacgcg cccctcctct ccgaccgcga ccgcggccac ccgccgctcg
      481 ccgagttcca ctcgccgttc cgcttcgggc cggcggagag ccgatgagcc gaaccgccct
      541 cctgacggga gtcaccggct tcatcggcgg caccctggcc cgccggctgc tggccgaggg
      601 gtggcgagtc cacgcgatcg tgcgtcccgg cagcgatgtc ggccgggtca cgccgcacga
      661 gcggctggcg cttcacgcgc atgacggcgg aacggagtcg ctgatccgcc tggtccagac
      721 ggcggcgccg gacgtcgcct tccacctcgc atccctgttc ctcgccgatc accgccccga
      781 ccagatcgag gcgctcgtcg cctccaatct cctgtttcca gtgcagctgg cggaagcgct
      841 gaccagaacc ggcgttctgc gactcgttaa cacgggcacc gcttggcagc atcgccactc
      901 cgagccctac ctcccggtca acctctacgc cgccaccaaa caggcgttcg aggacctgct
      961 cctttatcat cacgatgcgt gcggtctctc cgtcgtgact ctcaagctgt tcgacaccta
     1021 tggagcgggc gacccgcgcc gcaagctcat cggcatcctc ctcgaggcgg cccgcacagg
     1081 cgaggcgctc ccgatgtcgc ccggcgagca ggtgctcgac cttacccatg tcgaggacgc
     1141 ggtcgagggt tacctgatcg cggccgagcg gctgctcgcg agcaaggagc cgcttcggga
     1201 gacctatttc cttcccggcg agcggcaccg gctaagggac ctggtccggc tcgtcgagcg
     1261 ggctgtcggg cgcccgctgg acgtgaattt cggcgggcgc gcctatcggc cgcgggaagt
     1321 gatgaacccg atcgaggccg agagtgaccg gctcctgccg ggctggaccc gacggcgcga
     1381 ccttctgacg acgctgccgg agctgctcgc cccgtgacag ggtcgcccgg aattctccgt
     1441 cgccaccgcg ccgtcgccat cggcctggtt gcccagctcc tccaatatgg gggcgcattg
     1501 ctgatgctgc cgctggtcct cactcgcctg agcgcggctg aggtggcgat cttctacgtc
     1561 ttcctcacca ttcagggcct gtcgctgctc gccgacctcg gctttcagcc gacctttgcg
     1621 cgcgccttcg cctccgcttt tgccggcgcc tccgagct
//
LOCUS       DASWNU010000044         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_390416, whole genome shotgun sequence.
ACCESSION   DASWNU010000044 DASWNU010000000
VERSION     DASWNU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390416"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..997
                     /locus_tag="VGB57_00940"
     CDS             17..997
                     /locus_tag="VGB57_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY0129938.1"
                     /translation="MSGARGGDKPFVDSYLRKGLESGVELKAMAGTTEAAGGYAVPEE
                     IDSEIARLLTSISPIRAIANVVRVGSSGYRKLVTAGGTPSGWVSETAGRPETATPTFI
                     EVAPPFGELYANPAASQAMLDDAAFDVEAWLASEIATEFARAEGSAFVAGNGVNRPKG
                     FLAGPTSAQTDGSRPFGTLQYLATGASGAFPANHPGDRLIDLVQTLRPPYRQGASFVM
                     NSATASIIRKFKTSTGGFLWQPSLVAGQPDTLLGYPVVEAEDMPDVAADSLSVAFGNF
                     RAGYLIAERTETQILRDPYTNKPFVHFYATKRVGGQVANSEAIKLLKFAA"
     gene            1120..1665
                     /locus_tag="VGB57_00945"
     CDS             1120..1665
                     /locus_tag="VGB57_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HEY0129939.1"
                     /translation="MIEGEALVLPPEAAGAAKDYLRVERADEDSLIAGLVLAAAELCE
                     AFIGRPLVERGFTETISAGRAWRRLGNGPVRAILGVEALAAEGPAQALPSADYGIDID
                     SRGDGWVRLTATGEPRRVRVGYRAGLAGAWAELPAALRQGILRLAAHFYTSRAAEGAR
                     GGEPPAAVAALWRPFRRLRLR"
     gene            1678..1893
                     /locus_tag="VGB57_00950"
     CDS             1678..1893
                     /locus_tag="VGB57_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129940.1"
                     /translation="MFERLMLHGAALARKAAEGRRSSLAAELSDEAPEGVRVSEEEAG
                     VALSGRGLGRRFGLDPELRWLIAGRRR"
     gene            1890..2279
                     /locus_tag="VGB57_00955"
     CDS             1890..2279
                     /locus_tag="VGB57_00955"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEY0129941.1"
                     /translation="MSGAGAALRAAAIEALEALDLGGVYPGPPLQAAYPYATVECGPE
                     TDWSHKSGKGRELRLAVTLRDSGERPERAQAFAEVVEAAMEAGLDVEGWRLVTLALVR
                     ARTVAEGRGGSMGWAVAIDYRARMLAE"
     gene            complement(2416..2736)
                     /locus_tag="VGB57_00960"
     CDS             complement(2416..2736)
                     /locus_tag="VGB57_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129942.1"
                     /translation="MISQVLLLALMASAQGNLASARTSYSKCLGAFAREQVKAGSDEA
                     AFQAKLATACKAEEAAFRAASVASDVSAKIAKATAERNATEEIGYIHENAVETFKASL
                     ATPK"
     gene            2780..3187
                     /locus_tag="VGB57_00965"
     CDS             2780..3187
                     /locus_tag="VGB57_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY0129943.1"
                     /translation="MAAEKGSAFLLKVGNGGSPPVFATVAGMRTTQMSVNGEAVNVTS
                     KDSGGWRELLSGAGVRSVSVAASGIFTGSAAEVRIKSNALAGQVDDYELSFESGERLR
                     GRFLVTRLDYSGDYNGERNYTLSLESSGPVVNL"
BASE COUNT          480 a          961 c         1263 g          505 t
ORIGIN      
        1 ggcggcccgg ccggcgctga gcggggcgag gggaggggac aagcctttcg tcgattccta
       61 tctgagaaag gggctggaga gcggggtcga gctcaaggcc atggccggga cgaccgaggc
      121 tgcgggcggc tatgcggtgc ccgaggagat cgattcggag atcgcccggc tgctgacctc
      181 gatctcgccg atccgggcga tcgccaacgt cgtcagggtg ggaagctcgg gatacaggaa
      241 gctggtgacg gcggggggta ccccgtccgg ctgggtgagc gagacggcgg ggcggccgga
      301 gacggcgacc ccgaccttca tcgaggtcgc gcctccgttc ggcgagctct acgccaatcc
      361 ggcggcgagc caggcgatgc tcgacgatgc ggcgttcgac gtcgaggcct ggctggcgag
      421 cgagatcgcg accgagttcg cccgggccga gggctcggcc ttcgtcgccg gcaacggggt
      481 caaccggccg aagggcttcc tggcggggcc gacctcggcc cagacggatg gatcgaggcc
      541 gttcgggacc cttcaatatc tggcgaccgg agcgtcgggg gcgtttcccg ccaaccatcc
      601 gggcgacagg ctgatcgacc tcgtccagac tttgcggccg ccttaccgcc agggcgcgtc
      661 gttcgtgatg aactccgcca cggcgtcgat catccgcaag ttcaagacct cgaccggcgg
      721 gttcctgtgg cagccgagcc tggtggcggg gcagcccgac accctgctcg gctatccggt
      781 ggtcgaggcg gaggacatgc ccgacgtggc ggcggacagc ctgtcggtcg cgttcggcaa
      841 tttccgggcg ggctatctga tcgcggagcg gaccgagacc cagatcctcc gcgatcccta
      901 caccaacaag ccgttcgtcc atttctacgc gaccaagcgc gtgggcgggc aggtggcgaa
      961 ttcggaggcg atcaagcttc tgaagttcgc ggcgtgacgg ccgtcgcctt tcactgaccg
     1021 tcattgcgag gagtgtagcg acgaagcaat ccagcgggcc tccaaacccg actgggttgc
     1081 ttcgcttcgc tcgcaatgac ggtgcgaggg agaaagatca tgatcgaggg cgaggctttg
     1141 gtcctgccgc cggaggcggc gggggcggcg aaggattatc tgcgggtcga gcgggcggac
     1201 gaggactccc tgatcgccgg gctggtgctg gcggcggcgg agctatgcga ggctttcatc
     1261 ggccggccgc tggtggagcg cggctttacc gagacgattt cggcgggccg ggcgtggcgg
     1321 cggctgggca atgggccggt tcgggcgatc ctcggggtcg aggcgcttgc cgccgaaggg
     1381 cctgcacagg ccctgccgag cgccgactat ggaatcgaca tcgactcgcg cggcgacggc
     1441 tgggtccggc tgaccgccac cggcgagccc cggcgggtga gggtcggcta ccgggccggg
     1501 ctggccggcg cctgggcgga gctgccggcc gctctcaggc agggcatcct gcgactggcg
     1561 gcgcatttct acacgagccg ggcggcggaa ggcgcgcgcg ggggcgagcc gccggcggcg
     1621 gtcgccgcct tgtggcggcc gttccgccgg ctccggcttc gctaagggag gaagatcatg
     1681 ttcgaacgat tgatgcttca cggggccgcg ctcgcgcgca aggcggcgga ggggcgacgc
     1741 tcctcgctgg cggcggaatt gagcgacgag gcgcccgaag gcgtgagggt gagcgaggaa
     1801 gaggcggggg tggcgctttc gggccgcggc ctcgggcgcc gcttcgggct cgaccccgag
     1861 ctgcgctggc tcatcgccgg gaggcggcgg tgagcggggc gggcgcggcg ctgagggcgg
     1921 cggcgatcga ggcgctcgag gcgctcgatc tgggtggagt ctatccgggg ccgccgcttc
     1981 aggcggccta tccttatgcg acggtcgagt gcggccccga gaccgactgg agccacaaga
     2041 gcggcaaggg gcgcgagctc aggctggcgg tgacccttcg cgactcgggc gagcggccgg
     2101 agcgggcgca ggccttcgcc gaggtcgtcg aggcggcgat ggaagcgggg ctggacgtcg
     2161 agggctggcg gctggtgacg ctcgctttgg tccgagcgcg gaccgtcgcc gaagggcgcg
     2221 gcggatcgat ggggtgggcg gtggcgatcg actatcgggc caggatgctg gcggagtgat
     2281 ttaatcctcc ccgcgatttc gcggggaggg ggaccgcgcg aagcgcggtg gaggggttct
     2341 tcgaagtcag aaaggcccct ccaccaccct tcgggtggtc cccctcccct gcaaatgcag
     2401 gggaggaatt cggccttact tcggagtcgc gaggctggcc ttgaaggtct cgaccgcgtt
     2461 ctcgtggatg tagccgatct cctcggtcgc gttgcgttcg gccgtcgcct tggcgatctt
     2521 ggccgagacg tcgctggcga cggacgcggc tcggaaggcg gcttcctccg ccttgcaggc
     2581 ggtggcgagc ttggcctgga aggcggcttc gtcggagccg gccttcacct gctcgcgggc
     2641 gaaggcgccg agacatttgg aatagctggt gcgggccgaa gcgaggttgc cctgggcgga
     2701 cgccatgagg gccagaagca gcacttgtga aatcatcccg aattccagtg caacaagttg
     2761 agacgaaggg agaatatcga tggctgcgga aaagggaagc gctttcttgc tgaaggtcgg
     2821 aaatggcgga tcgccgccgg tcttcgcgac ggtggcgggg atgcggacga cccagatgtc
     2881 ggtgaacggc gaagcggtga acgtcaccag caaggattcg ggcggctggc gcgagctgtt
     2941 gtcgggggcg ggcgttcgct cggtctcggt ggcggcgagc gggatcttca ccggatcggc
     3001 ggcggaagtg cggatcaagt cgaacgcgct cgccggtcag gtcgacgatt acgagctgag
     3061 cttcgagagc ggcgagcgat tgcgcggccg cttcctcgtc acccggctcg attattcggg
     3121 cgattacaat ggcgagcgca attatacgct cagccttgaa agctccggcc cggtcgtaaa
     3181 cctgtgagcg gcgccaatcc agcgcgcgg
//
LOCUS       DASWNU010000045         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_287155, whole genome shotgun sequence.
ACCESSION   DASWNU010000045 DASWNU010000000
VERSION     DASWNU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287155"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VGB57_00970"
     CDS             <1..1535
                     /locus_tag="VGB57_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEY0129944.1"
                     /translation="LSTGLKMALIFSAALFPLGLIAILVSVQSAEQKTADRREETLAR
                     LQIKAQRLNSAFSRSVLTINTASVAVSLTPEGSQVCQTTLRQLEQGPVPVHYALYAAG
                     ELRCASAGFAPPPALPRAARSGTIARLHPDGSALSLFVFGEAGELEGIAEYGREALSD
                     LSYIRGTSPDFNLDLSQSGRQMVLRSDFKGGAWRQRVEGTESVAGGQLRLSILLSAVP
                     ITLADTLLILLPVLMWVFATLIGWLILNRLLLRPLARMERAVAAYRPGDRELALPRVA
                     TPAREIRELGEAFDRAVRTLAHHEAELEAAVERQTKLVREVHHRVKNNLQVVASLLNI
                     HSRGSPNEEVAAAYASIQRRVDALAVVHRNHFAELEENRGVALKPLISELAANLRATA
                     PASALGMSIRLSLEPYYVTQDVAVSVAFLITEIAEYAMLCGAGTVSIALTGEGASAGT
                     AQLAVESDSLRPGAPCDAILTERFERIVTGLSRQLRSTIDRDLEAGRYTLAVAVVDKA
                     DR"
     gene            1694..2185
                     /locus_tag="VGB57_00975"
     CDS             1694..2185
                     /locus_tag="VGB57_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129945.1"
                     /translation="MRYLLLVPLLLLAACGQDPETPEQAEQRERGARPPMPELEPPVE
                     TAQDREDKGDAAATLKLYYARIGSGDYDSAWAMRSGEAGGEAQRRFADNFRAYESYQA
                     DVGTPSEPVEAQGWAYVEVPVMIRGAFRGGKPFTSAGSVTMRRATSGTEAGRGWRIYT
                     GER"
     gene            2341..>2610
                     /locus_tag="VGB57_00980"
     CDS             2341..>2610
                     /locus_tag="VGB57_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0129946.1"
                     /translation="MTHRRRPIAYAAAGEQPFNTINTTPIIDVMLVLLIMFIITLPLM
                     THSVKIDLPGTGGGEPEIHQVALDARSRLSWDGVPISEAALPARLA"
BASE COUNT          403 a          950 c          855 g          402 t
ORIGIN      
        1 ggctctcgac cgggctcaag atggcgctca tcttcagcgc ggcgctgttc ccgctcggcc
       61 tcatcgccat cctcgtctcg gtccagagcg ccgaacaaaa gacggcagac cgccgcgagg
      121 agaccctcgc ccggctgcag atcaaggcac agcggctcaa ctcggccttt tcgcgcagcg
      181 tgctcaccat caataccgcc agcgtcgccg tttcgctgac gcccgagggc tctcaggtct
      241 gccagactac tttgaggcag ctcgagcagg gaccggttcc ggtccactac gccctctacg
      301 cggcgggcga gctgcgctgc gccagcgccg gcttcgcgcc gccgccggcc ctcccgcgtg
      361 cggcgcggtc gggcacgatc gcccggctcc atccggacgg ctccgcgctt agcctgttcg
      421 tcttcggaga ggcgggcgag ctcgagggaa tcgccgaata tggccgcgag gcgctgagcg
      481 acctctccta catccgggga accagccccg acttcaacct cgatctcagc caaagcgggc
      541 ggcagatggt gctgcgctcc gacttcaagg gcggggcgtg gcggcagcgc gtggagggca
      601 ccgaatcggt ggcggggggc cagcttcggc tcagcatcct cctctccgcg gttccgatca
      661 cgctcgcgga cactctgctt atcctgcttc cggtgctgat gtgggtcttc gcgaccctga
      721 tcggctggct gatcctgaac cgccttctgc ttcggccgct ggcgcgcatg gagcgggccg
      781 tggccgccta ccggcccggc gaccgcgagc tcgcgctccc ccgggtcgcc actccggcgc
      841 gggagatacg ggagctgggc gaggcgttcg accgggcggt ccggaccctc gcccatcacg
      901 aggcggagct cgaagcggcg gtcgagcgcc agaccaagct ggtccgcgaa gtccatcacc
      961 gggtgaagaa caaccttcag gtcgtcgcct cgctgctcaa catccactcg cgcggctcgc
     1021 ccaacgagga ggtcgccgcg gcctacgcct cgatccagcg ccgggtcgac gcgctcgccg
     1081 tcgtccaccg caaccatttc gccgagctcg aggagaatcg cggggtcgcc ttgaagccgc
     1141 tcatctccga gctcgccgcc aacctgcgcg ccaccgcccc cgcctccgcc ctcggaatga
     1201 gcatccgcct ttcgctggag ccttattacg ttacccagga cgtcgccgtc tctgtggcgt
     1261 tcctgatcac ggagatcgcc gaatatgcga tgctgtgcgg cgccgggacg gtcagcatcg
     1321 ccctgaccgg cgaaggcgcg agcgccggca cggcccagct cgctgtcgaa tcggacagcc
     1381 ttcgcccggg cgccccttgc gacgcgatcc tcaccgagcg gttcgagcgg atcgtcacag
     1441 gcctgtcgcg acagctccgc tcgaccatcg accgcgacct cgaggccggc cgctacaccc
     1501 tcgccgtcgc ggtggtggac aaggcggatc ggtgactaat cctccccgcg atttcgcggg
     1561 gagggggacc gcgcgaagcg cggtggaggg gttcttcagg ctcggcaagg cccctccacc
     1621 aggcttcgcc tggtccccct cccccggaaa tccgggggag gaatttcgaa ggccaacccg
     1681 ctataggccc cccatgcgct atctgctgct cgtccccctc ctcctcctcg ccgcctgcgg
     1741 gcaggacccc gagacgcccg agcaggcgga gcagcgcgag aggggcgcgc ggccgcccat
     1801 gcccgagctc gagcctccgg tggagacggc gcaggaccgc gaagacaaag gcgatgcggc
     1861 ggccaccctc aagctttact acgcccggat cggatcgggc gattacgatt ccgcctgggc
     1921 gatgcgctcg ggcgaggcgg gcggcgaggc gcaaagacgc ttcgccgaca atttcagggc
     1981 gtatgagagc tatcaggcgg acgtcggcac tccgagcgag cccgtcgagg cgcagggatg
     2041 ggcctatgtc gaggtgccgg tgatgatccg cggcgccttc cgcggcggca agccgttcac
     2101 cagcgcgggc agcgtcacga tgcgcagggc cacgtccgga accgaagcgg gccgcggctg
     2161 gcggatctac acgggcgagc gctaggtccg tcgtcccggc gaaggctggg accccggcgg
     2221 gtgatgaaca ccgtgccttt caccccccgg ggccccagcc tgcgctgggg cgacgatgag
     2281 gaacctattg ttcctaatcg tcgttgatgt tatgatgtta catcacatca ggaggccgag
     2341 atgacccacc gccgccgccc catcgcctac gccgccgccg gcgagcagcc tttcaacacg
     2401 atcaacacca ccccgatcat cgacgtcatg ctcgtcctcc tgatcatgtt catcatcacc
     2461 ctgccgctga tgacccacag cgtgaagatc gacctgcccg gcaccggcgg cggggagccg
     2521 gagatacacc aggtggcgct cgacgcccga agccggctga gctgggacgg agtgcctatc
     2581 tccgaggcgg cccttcccgc ccgtctcgcg
//
LOCUS       DASWNU010000046         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_79474, whole genome shotgun sequence.
ACCESSION   DASWNU010000046 DASWNU010000000
VERSION     DASWNU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79474"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1666)
                     /locus_tag="VGB57_00985"
     CDS             complement(<1..>1666)
                     /locus_tag="VGB57_00985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY0129947.1"
                     /translation="LTITDNGRGIPIDEQPRYPGKSALEVILTMLHSGGKFSNKAYAT
                     SGGLHGVGVSVVNALSSETIVEVAKNKELYRQSYSRGEPTSKLEPRGPTQNRRGTSIS
                     FVPDTEIFGEEARFKPARLYRLARSKAYLFAGVEIRWRCDPSLAEGDTPAEAVFQFPG
                     GLADHLKEQVGERNCATSDFFTGRQDFPDGLGLVEWAVAWPLWTDGGSSYYCNTIPTP
                     DGGTHEQGIRAALTRGIRGFAELVGQQKKAKEVQAEDVVGGAEVMLSVFVRDPQFQSQ
                     TKDRLTSPDAARLVEAAVRDHFDHYLADNMERGRALLAFVLDRMDERLKRRAEREIKR
                     KTATSGRKVRLPGKLTDCANSDDPAGTELFIVEGDSAGGSAKQARDRKTQAILPIRGK
                     ILNVASASADKIRGNQEIADLGLALGCGTRERCDPAQLRYDRIVIMTDADVDGAHIAT
                     LLMTFFFTEMGRIVEQGCLYLAQPPLYRLSSGPTVAYARDDAHRGELEASLFKGEKVE
                     ISRFTGLGEMNPSQLRETTMDPRSRSLIRVTLPDGYQQQHPVRDLVD"
BASE COUNT          238 a          529 c          609 g          290 t
ORIGIN      
        1 ggtcgacgag gtcgcggacg gggtgctgtt gctggtagcc gtcggggagg gtgacccgga
       61 tgagggagcg gctcctggga tccatggtcg tctcgcgaag ctgcgaaggg ttcatctcgc
      121 cgaggcccgt gaatcggctg atctcgactt tctcgccctt gaacaggctc gcctcgagct
      181 cgccgcgatg ggcgtcgtcg cgggcataag cgacggtcgg gcccgacgac aggcggtaga
      241 gcggcggctg ggccaggtag aggcagccct gctcgacgat cctgcccatc tcggtgaaga
      301 agaaggtcat cagcaaggtg gcgatgtggg cgccgtcgac gtcggcgtcg gtcatgatca
      361 cgatccggtc gtagcgaagc tgggccgggt cgcagcgctc gcgggtgccg cagcccaagg
      421 caaggccgag atcggcgatc tcctgattgc cccggatctt gtcggcgctg gccgaggcga
      481 cgttgaggat cttgccgcgg atcgggagga tcgcctgggt cttgcggtcc cgcgcctgct
      541 tggccgagcc gccggcgctg tcgccctcga cgatgaacaa ttcggtgccc gccggatcgt
      601 cgctgttggc gcagtcggtg agcttgccgg gaagccgcac cttgcgcccg gaagtcgcgg
      661 tcttgcgctt gatctcgcgc tcggcccgcc gcttcaggcg ctcgtccatc cggtcgagca
      721 cgaaggcgag gagggcgcgg ccgcgctcca tgttgtcggc gagataatgg tcgaaatggt
      781 cgcgcaccgc cgcttcgacg agccgggcgg cgtcggggct ggtcagccgg tccttggtct
      841 ggctctggaa ctgggggtcg cgcacgaaga cggagagcat gacctcggcg cccccgacga
      901 cgtcctcggc ctggacctcc ttcgccttct tctgctggcc gacgagctcg gcgaagccgc
      961 gaatgccgcg ggtcagcgcc gcccggatcc cctgctcgtg cgtgcctccg tccggggtcg
     1021 ggatggtgtt gcaataataa gaggagccgc cgtcggtcca cagcggccag gccaccgccc
     1081 attcgaccag cccaaggccg tccgggaaat cctgccggcc ggtgaagaag tcgctggtgg
     1141 cgcagttgcg ctcgcccacc tgctccttga gatggtcggc gaggccgccg gggaactgga
     1201 acaccgcctc ggcgggcgtg tcgccctcgg ccagcgaggg atcgcagcgc cagcgaatct
     1261 cgaccccggc gaacagatag gccttggagc gggcgagccg gtagagccgg gccggcttga
     1321 acctggcctc ctcgccgaaa atctcggtgt cggggacgaa gctgatgctg gttccgcggc
     1381 gattctgggt gggtcctcgc ggctcgagct tcgaggtcgg ctccccgcgc gagtaggact
     1441 gccggtagag ctccttgttc ttggccacct cgacgatcgt ctcgctcgac aaggcgttga
     1501 ccacgctcac tccgactccg tggaggccgc cggacgtcgc ataggccttg ttcgagaact
     1561 tgccgcccga atggagcatg gtgaggatga cctcgagggc cgacttgccc ggatagcggg
     1621 gttgctcgtc gatcgggatg ccgcggccgt tgtcggtgat cgtgag
//
LOCUS       DASWNU010000047         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_494569, whole genome shotgun sequence.
ACCESSION   DASWNU010000047 DASWNU010000000
VERSION     DASWNU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494569"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="VGB57_00990"
     CDS             <1..1672
                     /locus_tag="VGB57_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY0129948.1"
                     /translation="EHYAWLLRESPTYATALGVRDHDDRIEDISLAARDRQAREAAAF
                     LERLNRIPAEALSPADRVNRAILKRGLEEGIEANRFPQRVMLFTTYAGWHQAFAGLAD
                     NLPFRTRADYESYLTRLALYPRQNDAALDITGQAVRGGFTLPCSVLGNYEKGIAGVIA
                     EDPAKSRFYEPFTRTRPGDVGEAEWARMQARARRLVAEVLNPAYARHLAFYRNIYLPK
                     CAKADSISAQPGGREYYAFRVRQETTTSLTPDQIHEIGLHESARIRAEMDKVARSAGF
                     AGREAFIRELRTNSKYYATSPAQLMQAAATTAKTIDGKMPGFFATLPRLPYGVREIPA
                     EIAEGTTTAYYGQGSPASGLAGTYYVNTSKLDQRPLWELPALTLHEAVPGHHHQISLQ
                     QEVELPPFRRHFTFFTAFTEGWGLYAESLGEEMGLYDTPEKKMGQLSYQAWRASRLVV
                     DTGIHSKDWDKARAVAFMRENTALTDANIDAEVNRYISWPGQALGYKIGELRIKALRA
                     KAEKALGARFDLRRFHDAVLLQGSVPLDVLEAQVDAWVEAEKILPPRA"
     gene            complement(1820..2233)
                     /locus_tag="VGB57_00995"
     CDS             complement(1820..2233)
                     /locus_tag="VGB57_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129949.1"
                     /translation="MKAALSIAALGAASAAALTLIPVPASAQGAQQERVRRLVVYGRD
                     PCPRAQSGDEIVVCARRPETERYRIPRELRDAATADDPDSTSWAARAEALEYVGRTGI
                     QSCSTVGPGGVSGCWNEMVRAWRRDRRQGEGEPGR"
     gene            complement(2384..2881)
                     /locus_tag="VGB57_01000"
     CDS             complement(2384..2881)
                     /locus_tag="VGB57_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006958005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129950.1"
                     /translation="MKALLLAAGAAGALLAAAPAAAQGAAAEPKVSTMIVYGDDPCPP
                     STDDTIIVCGRQPETERFRIPAPLRGDPNDPANQAWATRSQALEVAGRSGIGSCSPVG
                     PGGASGCFNQIVQAARAERRGDPSVNWNALVEQARRERLGKIDAQAEAEEADAKADEA
                     AATPR"
     gene            complement(2886..3374)
                     /locus_tag="VGB57_01005"
     CDS             complement(2886..3374)
                     /locus_tag="VGB57_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY0129951.1"
                     /translation="MVRVDQAGEYGATRIYCGQLAVLGSHPASRPIARMQSQEERHLR
                     TFDEMMVDRGIRPTLLQPVWNVAGHALGAATALMSPSAAMACTAAVETEIDRHYSDQL
                     RALGHEEPDLADNIRRFRAEELEHRDTAMAAGAEDAFGYPILSSLIRAGCRIAIALSK
                     RI"
     gene            complement(3440..3931)
                     /locus_tag="VGB57_01010"
     CDS             complement(3440..3931)
                     /locus_tag="VGB57_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY0129952.1"
                     /translation="MTSLDTARTLAIAVPAALLAGAYGSEIWGGLYPCEMCWWQRYAH
                     FAALGLALLSFLLVKAPDRGRSFVWLAALAILVSGGIGAYHAGVEAGVFEGFTTCTST
                     GTAGLSAEDMLKEIMAKPMVRCDDIQFSFLGVSMAGWNAILSILAALSILWLSLRRPR
                     ASA"
     gene            complement(4026..>4988)
                     /locus_tag="VGB57_01015"
     CDS             complement(4026..>4988)
                     /locus_tag="VGB57_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0129953.1"
                     /translation="LTHLNGKLIYGGTLDEAVDSMRGPPGSSIKVTILRLGRDKPFDV
                     TLNRAAIELKAVKWSVKDNVGVINVNTFSRTTTDETLAAIAAIEKQLGRPPLGYIVDL
                     RSNPGGLLDQAVGLSDVFLERGEIVSQRGRRKTDIERYFAKPGDAARGLPIIVLVDAG
                     SASAAEIVAAALQDHHRGLVMGERSFGKGSVQTLVPLSQKTGLRLTTARYYTPSGRSV
                     QEGGIKPDIEVPQLSDPDYKSRPKLREADLRRHLVNEVTVDNDVIEDDGKPDPRFAAT
                     PEQLKAKGIEDFQLNYALQTISRLGRNAAVAPAAGGGARPTGKR"
BASE COUNT          763 a         1639 c         1799 g          787 t
ORIGIN      
        1 cgaacattat gcctggctgc tgcgcgagag cccgacctac gcgaccgcgc tcggcgttcg
       61 cgaccatgac gaccggatcg aggatatcag cctcgccgcc cgcgaccggc aggcgcggga
      121 ggccgccgcc ttcctggagc gactgaacag gatcccggcc gaggcgctgt cgccggcgga
      181 ccgcgtcaac cgggcgatcc tgaagcgcgg gctggaggag gggatcgagg ccaaccgctt
      241 cccccagcgg gtgatgctgt tcaccactta tgccggctgg catcaggctt tcgcgggcct
      301 cgccgacaac ctgccgttcc ggaccagggc ggattacgaa agctatctga cccgcctcgc
      361 cctctatccc aggcagaacg acgccgcgct cgacatcacc gggcaggcgg tgaggggcgg
      421 cttcactctg ccctgctcgg tgctgggcaa ttacgagaag ggcatcgccg gagtgatcgc
      481 cgaggatccg gcgaagtcgc gtttctacga gcctttcacc cgcactcggc ccggcgacgt
      541 cggcgaggcc gagtgggcgc ggatgcaggc gcgggcgcgg cggctggtcg ccgaggtgct
      601 caacccggct tatgccaggc atctcgcttt ctaccgcaat atctacctgc ccaaatgcgc
      661 caaggccgac agcatctcgg cgcagccggg agggcgcgaa tattacgcct tccgggttcg
      721 gcaggagacg acgacctcgc tcaccccgga ccagatccac gagatcgggc tccacgaatc
      781 ggcgcggatt cgggcggaga tggacaaggt ggccaggtcc gccggcttcg ccggccgcga
      841 ggcgttcatc cgcgagctca ggaccaattc gaaatattat gcgacgagcc ccgcgcagct
      901 gatgcaggcg gcggcgacca cggccaagac gatcgacggc aagatgccgg gcttcttcgc
      961 gaccctgccg aggctgccct atggagtgcg cgagataccg gccgagatcg cggaagggac
     1021 gacgaccgct tattacggcc agggctcgcc cgcgagcggc cttgccggca cctattacgt
     1081 caacacctcg aagctcgacc agaggccgct ctgggagctt ccggcgctga ccctgcacga
     1141 ggcggtgccc ggccatcacc accagatctc gctccagcag gaggtcgagc ttccgccctt
     1201 ccgccgccac ttcaccttct tcaccgcctt caccgagggc tggggcctct atgccgagag
     1261 cctgggcgag gagatggggc tgtacgacac tccggagaag aagatgggcc agctctccta
     1321 ccaggcatgg cgggcctcgc ggctggtcgt cgacacgggg atccattcga aagactggga
     1381 caaggcccgg gcggtggcct tcatgcgcga gaacaccgcc ttgaccgacg ccaatatcga
     1441 cgcggaggtc aatcgctaca tcagctggcc cggccaggcg ctcggctaca agatcggcga
     1501 attgaggatc aaggcgcttc gcgcgaaggc cgagaaggcg ttgggggcca ggttcgacct
     1561 gaggcgcttc catgacgccg tgcttctgca ggggagcgtt ccgctggacg tactcgaggc
     1621 gcaggtcgat gcatgggtgg aagcggagaa gatcttgcct ccccgcgcgt agcgggggga
     1681 gggggaccgc ccgaagggtg gtggaggggt agctgtcgtc gactggagtg ttcgcgccgc
     1741 agtctcctgg accagcgcct tctacccctc caccatgctt cgcatggtcc ccctccccat
     1801 ctggcgatgg ggaggcaaat cagcgccccg gttccccttc cccctgccgg cggtcgcggc
     1861 gccaggcccg gaccatctcg ttccagcagc cgctgactcc gccggggccg acggtcgagc
     1921 agctctggat tccggtgcgg ccgacatatt cgagcgcctc cgcgcgggcg gcccagctgg
     1981 tgctgtccgg gtcgtccgcg gtggccgcgt cgcgaagctc cctggggatt cggtagcgtt
     2041 cggtctcggg ccggcgggcg cagacgacga tctcgtcgcc gctctgggcg cggggacagg
     2101 ggtcccggcc gtagacgacg aggcgccgga ctcgttcctg ctgtgctccc tgggcactcg
     2161 ccggaaccgg gatcaaggtc aaagcggcgg ccgaagcggc gccgagggcg gcgatggaca
     2221 aggcggcttt catgcgcggc ctcctttcgg ttcaaggaaa gaagggccga aaggctggga
     2281 ggttccactc cagcacccgt catcccggcg aaagccggga cctcaggtcg agaaggctct
     2341 gcgggtctcc ccgaggtccc ggctttcgcc gggatgacgg ccgtcagcgc ggagtcgccg
     2401 ccgcttcgtc cgccttggcg tcggcttcct cggcttcggc ctgggcgtcg atcttgccga
     2461 gccgttcgcg ccgagcctgt tccacgaggg cgttccagtt gacgctggga tcgccccggc
     2521 gctcggcgcg ggccgcctgc acgatctggt tgaagcagcc gctggccccg cccggtccga
     2581 ccggcgagca gcttccgatc ccggagcggc ccgcgacctc gagcgcctgc gagcgggtcg
     2641 cccaggcctg gttggccgga tcgttgggat cgccgcgcag aggcgcgggg atacggaagc
     2701 gctccgtttc gggctggcgc ccgcagacga tgatcgtgtc gtccgtgctg ggcgggcagg
     2761 gatcgtcgcc atagacgatc atcgtgctga ccttcggctc ggcggcggct ccctgggcgg
     2821 cggcgggggc ggccgcgagc agggcgccag cggcgccggc ggcgaggagg agggctttca
     2881 tcgattcaga tccttttcga cagcgcgatg gcgatacgac agccggcgcg gatgagggaa
     2941 gacaatatgg gatagccgaa cgcgtcctcg gcgccggcgg ccatcgcggt gtcgcgatgc
     3001 tcgagctcct cggcgcggaa gcggcggata ttgtcggcga ggtcgggctc ctcatgcccg
     3061 agggcccgga gctggtcgga ataatggcgg tcgatctcgg tctcgacggc ggcggtgcag
     3121 gccatggccg cgctggggct catcagcgcc gtcgccgctc ccaaagcgtg gccggcgacg
     3181 ttccagacgg gctggagcag ggtcggtcgt attccgcgat cgaccatcat ctcgtcgaac
     3241 gtcctgaggt gcctttcctc ctgcgactgc atccgggcga tgggacggct cgccgggtgc
     3301 gagccgagca cggcgagctg gccgcaatag atacgggtcg ctccatattc gccggcctgg
     3361 tcgacccgga ccatcgaatc gacgtccggc cgctcgtcgc cggggcgcca gcgggggcgg
     3421 gaaggggctt gggcggcgtt cacgcgctcg ccctcggtcg cctgaggctc agccatagga
     3481 tggacaacgc ggcaaggatg gaaaggatcg cattccatcc ggccatcgag actccgagga
     3541 agctgaactg gatgtcgtcg cagcgaacca tgggcttggc catgatctcc ttcagcatgt
     3601 cctcggcgct gaggccggcg gtgccggtcg aggtgcaggt ggtgaagccc tcgaatactc
     3661 cggcttcgac cccggcgtga taggcgccga tgccgccgct gacgaggatc gccagcgcgg
     3721 cgagccagac gaagctccgg ccccggtccg gggccttgac gagcaggaac gacagcagcg
     3781 ccagcccgag cgccgcgaaa tgggcgtaac gctgccacca gcacatctcg cagggataga
     3841 ggccgcccca tatctcggag ccgtaggcgc cggcgagcag ggcggcgggg acggcgatgg
     3901 cgagggtgcg cgccgtgtcg aggctcgtca cggcacgtca tcccggcgga agccgggacc
     3961 tcggggagac ccgccgggac ttctcgtccc gaggccccgg ctttcgccgg ggtgacggtt
     4021 ctgcatcagc gctttccggt cggccgagcc ccgccgccgg ccgccggcgc caccgcggcg
     4081 ttgcggccga ggcgggagat ggtctggagc gcgtaattga gctggaagtc ctcgatgccc
     4141 ttggccttca attgctccgg cgtggcggca aagcgcggat cgggcttgcc gtcgtcctcg
     4201 atcacgtcgt tgtccaccgt cacctcgttg accaggtggc gccgcaaatc ggcctcgcga
     4261 agcttgggcc gcgacttgta atcgggatcg gaaagctgcg gaacctcgat gtcgggcttg
     4321 atcccgcctt cctgcaccga gcggcccgac ggggtgtaat agcgggccgt ggtgaggcgg
     4381 aggccggtct tctggctcag cggaacgagg gtctggaccg agcccttgcc gaagctgcgc
     4441 tcgcccatga cgaggccgcg atggtggtcc tggagcgccg ccgcgacgat ctcggccgcc
     4501 gaggcgctgc cggcgtcgac gaggacgatg atcggaaggc cgcgggcggc gtcgcccggc
     4561 ttggcgaaat agcgctcgat gtcggtcttc ctgcggccgc gctgagagac gatctcgccg
     4621 cgctcgagga acacgtcgga gaggccgacg gcctggtcga gaagcccgcc cgggttcgag
     4681 cgcaggtcga cgatgtagcc gagcgggggc cggccgagct gcttttcgat ggcggcgatg
     4741 gcggccagag tctcgtccgt ggtggttcgg ctgaaggtgt tgacgttgat gacgccgaca
     4801 ttgtccttca ccgaccattt gaccgccttg agctcgatgg cggcgcgatt cagggtgacg
     4861 tcgaacggct tgtcgcggcc gaggcggagg atggtgacct tgatcgagct gcccggcggg
     4921 ccgcgcatcg aatcgaccgc ctcgtcgagg gtcccgccgt agatcagctt gccgttcagg
     4981 tgggtgag
//
LOCUS       DASWNU010000048         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_157025, whole genome shotgun sequence.
ACCESSION   DASWNU010000048 DASWNU010000000
VERSION     DASWNU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157025"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2074
                     /gene="parE"
                     /locus_tag="VGB57_01020"
     CDS             95..2074
                     /gene="parE"
                     /locus_tag="VGB57_01020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY0129954.1"
                     /translation="MSDLFASTAAPSSEYDAHSIEVLEGLEPVRRRPGMYIGGTDERA
                     LHHLAAEVLDNAMDEAVAGFATRIEVTLEAGNRLTITDNGRGIPIDEHPRYPGKSALE
                     VILTMLHSGGKFSNKAYATSGGLHGVGVSVVNALSSETVVEVARNKELWRQTYSRGVP
                     VSKLEPRGPTQNRRGTSLSFVPDTEIFGEEARFKPARLYRLARSKAYLFAGVEIRWRC
                     DPALVEGDTPAEAVFQFPGGLADHLKEQVGTRNCATSDFFTGRQDFPGGQGMVEWAVA
                     WPLWTDGGSSYYCNTIPTPDGGTHEQGIRAALTRGIRAFAELVGQQKKAKEVQAEDVV
                     GGAEVMLSVFVRDPQFQSQTKDRLTSPDAARLVEAAVRDHFDHYLADNMERGRALLAF
                     VLDRMDERLKRRQEREIKRKTATSGRKLRLPGKLTDCANDDPAGTELFIVEGDSAGGS
                     AKQARDRKTQAILPIRGKILNVASASADKIRGNQEIADLTLALGCGTRERCDPAQLRY
                     DRIIIMTDADVDGAHIATLLMTFFFNEMGRIVEQGCLYLAQPPLYRLSSGPTVAYARD
                     DAHRGELEATLFKGKKVEISRFKGLGEMNPSQLRETTMDPKSRSLIRVTLPDGYQPQQ
                     PVRELVDRLMGRNPEHRFNFIQRHAAEIAEEEIDA"
     gene            2187..3452
                     /locus_tag="VGB57_01025"
     CDS             2187..3452
                     /locus_tag="VGB57_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0129955.1"
                     /translation="MRWLLGLLLLICAVPIQAAEPSAQLSRRGKDLVRVLNGETPPRQ
                     FFSPAFLAKVPPAQVAAFAAQLKASYGAAREIRSIEAGGPNAGTLLVAFERAVVRLQM
                     AVGPQPPHLVEGLLVVGAEASAADVAAVFGELVGLPGDVSLRAARLEDAGPADFLTEQ
                     AERPLAIGSAFKLFVLAELVRSVKAGERRWSDVVPLGTPSLPSGIVQDWPKGSPVTLH
                     SLAALMISRSDNSATDTLLGVLGRDKVERLLPELGVRAPERNRPFLSTREAFALKLGD
                     PTLLARWKAADEAGRRALLPQLERIDASKLDPSRLGGRPAEIATVEWFASPADLVRTL
                     DWLRRSGDRTALDLLAINPGLGRDLANDFGYFGFKGGSEAGVLNLSFLLKSRSGRWMA
                     VSATWNNEAASLDEPRFVALTSRLLTLLK"
BASE COUNT          547 a         1258 c         1147 g          533 t
ORIGIN      
        1 cccccggccg caagtaccac ttgcggacga agcttgtgga tagaacaacc ccggaacctt
       61 gaacccgccg cccccctccc cctatacctc ccccatgtca gacctgttcg cgtccaccgc
      121 cgccccgtcc tccgaatacg acgcccattc gatcgaggtg ctcgaggggc tggagcccgt
      181 ccgccgccgg ccgggaatgt atatcggcgg gacggacgag cgggcgctcc accatctcgc
      241 cgccgaggtg ctcgacaatg cgatggacga ggccgtcgcc ggcttcgcca cccgaatcga
      301 ggtgacgctc gaggccggta accggctcac gatcaccgac aacggccgcg gcatcccgat
      361 cgacgagcat ccccgctatc cgggcaagtc ggcgctcgag gtgatcctga ccatgctcca
      421 ttcgggcggc aaattctcca acaaggccta tgcgacctcg ggcggcctgc acggcgtcgg
      481 agtcagcgtg gtcaacgctt tgtcgagcga gacggtggtc gaggtcgccc gcaacaagga
      541 gctctggcgc cagacctatt cgcggggcgt gcccgtttcg aagctggagc cgcgcggccc
      601 cacccagaac cgccgcggaa cgagcttgag cttcgtcccc gataccgaga tcttcggcga
      661 ggaggcgagg ttcaagccgg cacggctcta ccggctcgcc cgctccaagg cctatctgtt
      721 cgcgggcgtg gagattcgct ggcgctgcga tcccgcgctg gtcgagggcg acacgcccgc
      781 cgaggccgtc ttccagttcc ccggcggcct cgccgaccac ctcaaggagc aagtggggac
      841 acgcaattgc gcgaccagcg acttcttcac cgggcgccag gacttcccgg gcgggcaggg
      901 catggtcgaa tgggcggtgg cctggccgct ctggaccgac ggcggctcct cttattattg
      961 caacaccatc ccaacccccg acggcggcac ccacgagcag gggatccgcg ccgctttgac
     1021 ccgaggcatc cgagccttcg ccgagctggt cggccagcag aagaaggcga aggaggtcca
     1081 ggccgaggac gtggtcggag gcgccgaggt gatgctctcc gtcttcgtcc gggatcccca
     1141 gttccagagc cagaccaagg accggctgac cagccccgac gccgcccggc tggtcgaggc
     1201 ggccgtgcgc gaccatttcg accattatct cgccgataat atggagcgcg gccgggccct
     1261 gctcgccttc gtcctcgaca ggatggacga gcggctgaag cggcgccagg agcgtgagat
     1321 caagcgcaag accgcgacca gcggccgcaa gctccgtctt ccgggcaagc tcaccgactg
     1381 cgccaacgac gatccggcgg ggaccgaatt gttcatcgtc gagggcgaca gcgccggcgg
     1441 ctccgccaag caggcgcgcg accgcaagac ccaggcgatc ctgcccatcc gcggcaagat
     1501 attgaacgtc gcctcggcca gcgccgacaa gatccggggc aaccaggaga tcgccgatct
     1561 caccctcgcc ttgggctgcg gcacccgcga gcgctgcgat ccggcgcagc tccgctacga
     1621 ccggatcatc atcatgaccg acgccgacgt cgacggcgca catatcgcca ccctcctgat
     1681 gacgttcttc ttcaacgaga tgggcaggat cgtcgagcag ggctgcctct atctggccca
     1741 gccgccgctc taccgcctgt cgtcggggcc gaccgtcgct tatgcccgcg acgacgccca
     1801 tcgcggcgag ctcgaggcca ccctcttcaa gggcaagaag gtcgagatca gccgcttcaa
     1861 gggtctcggc gagatgaacc cgtcgcagct tcgcgagacc accatggatc ccaagtcccg
     1921 ctcgctgatc cgggtcaccc tccccgacgg ctatcagccg cagcagcccg tgcgcgagct
     1981 ggtcgaccgg ctgatgggcc gcaacccgga gcaccgcttc aacttcatcc agcgccacgc
     2041 ggccgagatc gccgaggagg aaatcgacgc gtgaggcggc gggggccgct cccggcgatg
     2101 gcctcggtgg cgcccggctg cgtctctgct ccgcccggat cggcccctcc tccgcttatg
     2161 gccccgtgac tggaggtcga gcccggttgc gctggctgct cggcctgttg ctgctgatct
     2221 gcgccgtccc gatacaggcg gcggagcctt ccgcccaact cagccgccgg ggaaaggatc
     2281 tggtgcgggt tctgaacggc gagacgcctc cgcggcaatt cttctccccc gcctttctgg
     2341 cgaaggtacc gcccgcgcaa gtggccgcct tcgccgcgca gctgaaggcg agctatggcg
     2401 ccgccaggga gataagatcg atcgaggccg gcggtccgaa cgccggcacc ttgctcgtcg
     2461 ccttcgagcg cgccgtcgtg cggctccaga tggcggtggg cccgcagccc ccgcacctcg
     2521 tcgaaggatt gctcgtggtc ggagcggaag cctccgcggc ggacgtggcg gccgtgttcg
     2581 gcgagctggt cggcttgccg ggcgacgtgt cgctgcgggc ggcgcggctg gaggacgcag
     2641 gccctgccga cttcctcacg gagcaggcgg agcggccgct cgcgatcggc tccgccttca
     2701 agctgttcgt gctcgccgag ctggtgcgct cggtgaaagc cggcgagcgg cggtggagcg
     2761 acgtcgtccc gctggggacc ccttccctgc cctccggtat cgtccaggac tggccgaaag
     2821 gctcgcccgt caccctccac agcctcgccg ccctgatgat ctcgaggagc gacaacagcg
     2881 ccaccgacac tttgctgggc gtgctcggcc gggacaaggt ggagcggctt ctgcccgagc
     2941 tcggggtgcg ggcgccggag cgcaaccgcc ccttcctctc gacccgcgag gccttcgccc
     3001 tcaagctggg tgatcccacc ttgctggccc ggtggaaagc cgccgacgag gcgggccggc
     3061 gggcgctact gccgcaactg gagcggattg atgcctcgaa gctcgacccc tcacggctcg
     3121 gcggccgccc cgcggagatc gccaccgtcg aatggttcgc ctcgccggcc gacctcgtcc
     3181 gaaccctcga ctggctgcgc cggagcggcg accggacggc gctcgacctg ctggcgatca
     3241 atcccggcct gggccgcgac ctggcgaacg atttcggcta tttcggcttc aagggcggct
     3301 ccgaagccgg agtgctgaac ctgagcttcc ttctgaagtc ccgctccggc cgctggatgg
     3361 ccgtctcggc gacgtggaac aacgaggcgg cttcgctcga cgagccccgc ttcgtcgccc
     3421 tgacgagccg gctgctgacc ttgctgaaat aaattcctcc ccgagatttc tcggggaggg
     3481 ggacc
//
LOCUS       DASWNU010000049         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_364903, whole genome shotgun sequence.
ACCESSION   DASWNU010000049 DASWNU010000000
VERSION     DASWNU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364903"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /gene="pgi"
                     /locus_tag="VGB57_01030"
     CDS             complement(<1..1391)
                     /gene="pgi"
                     /locus_tag="VGB57_01030"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0129956.1"
                     /translation="MDENQAWAAVEAAEPQTLAGLFAAEPDRLARLALDLPGLHFDFA
                     KTHLSAPLVAAFEALAQARGLAAARDALFAGAIVNPSEGRAAEHSAERGQGAPESVAR
                     ASGLRARMRALIDAIEAEAFGPIRHILHIGIGGSALGPDFVIDALGRDSDRYDTAIVS
                     NVDGAALEEAVAGFDPHSTLLVLASKTFTTLETLLNARSALAWMEQGGVADPYGRIVA
                     LTASPEKALEFGIDETRILPFAESVGGRYSLWSSIGFPIALALGWEAFETLLEGAAAM
                     DRHFRLAPLRENAPVLAAFADRYYANVRGSQTRAVFAYDERLRLLPSYLQQLEMESNG
                     KSVKLDGAPAGRATSLVTWGGVGTDAQHAVFQLLHQGSHLVPVEFVACVEPDHGLDEE
                     HHRQLLINCFAQGAALMSGKGSDDPHRHYPGDRPSTTILLDRLDPRTLGALLAFYEQR
                     TFANAVLMGINPF"
     gene            1485..2300
                     /gene="lepB"
                     /locus_tag="VGB57_01035"
     CDS             1485..2300
                     /gene="lepB"
                     /locus_tag="VGB57_01035"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0129957.1"
                     /translation="MASEAAKPGTKKKGGLAETTRFLLLFFLFAILLRTFVVAPFVIP
                     SGSMMPELRIGDYLFVAKWPYGYSRYSIPFGLASFGGRIWASEPKRGDVVVFRYPGED
                     EDYVKRLIGLPGDTVQMRGGQLFLNGKPVPKVRIADWLMPVTPNSPCRYAGRVQSPSV
                     EAGKPYCRYPRFRETLPGGRSYEVLDQFEGGADETPVYSVPAGHYFMMGDNRDDSADS
                     RFSLEQRGVGLLPSDYVIGRALIAFFSTDGSAEWIKPWTWFSAARWERVGRTF"
BASE COUNT          373 a          731 c          871 g          370 t
ORIGIN      
        1 tcgaacggat tgatgcccat caacaccgcg ttggcgaagg tgcgctgctc gtagaaagcc
       61 agcaaagcgc ccagcgtccg cgggtcgagc cggtcgagga ggatcgtcgt cgacggccgg
      121 tcgcccggat aatgacgatg gggatcgtcg ctgcccttgc cggacatcag ggccgcgccc
      181 tgggcgaagc agttgatgag gagctggcgg tgatgctctt cgtccaggcc gtggtcgggc
      241 tcgacgcagg cgacgaattc gaccggcacg aggtggctgc cctggtggag gagctggaag
      301 acggcgtgct gggcgtcggt gcccactccg ccccaggtga cgagcgaggt cgcccgaccg
      361 gcgggggcgc cgtcgagctt caccgacttg ccgttcgact ccatctcgag ctgctggaga
      421 taggagggca ggagccgaag ccgttcgtca taggcgaaga cggcgcgggt ctggctgccg
      481 cggacgttgg cgtaatagcg gtcggcgaag gcggcgagga cgggggcgtt ttcccggagg
      541 ggcgcgagcc ggaaatggcg gtccatcgcc gccgcccctt cgagcagggt ctcgaacgct
      601 tcccagccca gcgcgagggc gatcggaaag ccgatcgagg accagagcga atagcggccg
      661 ccgacgctct cggcgaaagg caggatccgg gtctcgtcga tcccgaattc gagcgctttc
      721 tcaggggaag cggtgagggc gacgatccgg ccgtagggat cggcgactcc gccttgctcc
      781 atccaggcga gggcggatcg ggcgttgagc agggtctcga gggtggtgaa ggtcttggac
      841 gccaggacga ggagggtgga gtgggggtcg aagccggcga cggcctcctc gagggcggcg
      901 ccgtcgacat tggagacgat cgcggtgtcg taacgatccg aatcgcggcc gagcgcgtcg
      961 atcacgaaat cggggccgag cgccgagccg ccgatgccga tgtggaggat gtggcgaatg
     1021 ggaccgaagg cctcggcctc gatcgcgtcg atgagggcgc gcatccgggc ccgaagaccc
     1081 gaggcccggg cgacgctctc cggagcgccc tggccgcgct cggcgctgtg ctcggcggcc
     1141 cgcccttcgc tcgggttgac gatggcgccc gcgaacaggg cgtcgcgcgc tgccgcgagg
     1201 ccgcgcgctt gcgccagggc ctcgaacgcg gcgacgagcg gggcgctgag gtgcgtcttg
     1261 gcgaagtcga aatggagccc gggcaggtcg agcgcgaggc gggccagacg atcgggctcg
     1321 gccgcgaaca ggccggcgag ggtttgcggc tcggccgctt cgacggcggc ccaggcttgg
     1381 ttttcgtcca tgagcgggct cttagtgagg cggcgaggcg atgccaatga ccgctgggac
     1441 gacgatggct tgaccgcacc cgcgccccaa gcgaaagcgg cggcatggcg agtgaagcgg
     1501 cgaagcccgg gacgaagaag aaaggcgggc tcgccgagac gacgcgattc ctgctgctct
     1561 tcttcctgtt cgcgatcctg cttcggacct tcgtcgtcgc gccgttcgtg atcccttcgg
     1621 ggtcgatgat gcccgagctc aggatcggcg actatctgtt cgtcgccaaa tggccctacg
     1681 gctattcgcg ctactcgatc ccgttcggcc tcgcctcgtt cggggggcgt atctgggcct
     1741 cggagccgaa gcgcggcgac gtcgtcgtct tccgctatcc gggggaggac gaggattatg
     1801 tgaaaaggct gatcgggctt ccgggcgaca cggtgcagat gcgcggtggc cagctcttcc
     1861 tcaacggcaa gccggtgccg aaggtcagga tcgccgactg gctgatgccg gtcacgccca
     1921 acagcccctg ccgctatgcc ggtcgggtcc aatcgccctc ggtcgaggcc ggaaagccct
     1981 attgccgcta tccgcgcttc cgcgagaccc tgcccggcgg ccgttcctac gaggtgctcg
     2041 accagttcga aggcggcgcc gacgaaacgc cggtctattc cgtccccgcc ggccattatt
     2101 tcatgatggg cgacaatcgc gacgacagcg ccgacagccg cttcagcctc gagcagaggg
     2161 gggtgggatt gcttccgagc gactacgtga tcggccgggc tctgatcgcc ttcttctcca
     2221 ccgacggctc ggccgagtgg atcaagccct ggacctggtt ctcggcggcg cgctgggaac
     2281 gggtggggcg caccttctga tggataaaac caccatcacc tggctggagg aaaccctcgg
     2341 ccggg
//
LOCUS       DASWNU010000050         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_209720, whole genome shotgun sequence.
ACCESSION   DASWNU010000050 DASWNU010000000
VERSION     DASWNU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209720"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGB57_01040"
     CDS             <1..330
                     /locus_tag="VGB57_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEY0129958.1"
                     /translation="CFGLGAWEFPVAAYYHSVRLKQPEPLPPAGRSFAALVRTNYVTR
                     TIPLGEPQYLFLEAMAEGGGVDDGLEAAARFLATTPEKVRQSWHSSGDRQRWIDWGFF
                     IDGDQTR"
     gene            371..880
                     /locus_tag="VGB57_01045"
     CDS             371..880
                     /locus_tag="VGB57_01045"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEY0129959.1"
                     /translation="MDEVKRSFPPVADARTRVLVLGSLPGEESLARSQYYANPRNHFW
                     RLIGAVVGAELAPLPYEVRLEALLEARIGLWDTVGSATRRGSLDGAIRGHLANDLADL
                     AARLPELRAVGFNGGKSASLGMAQLAGRPDLALIPLPSSSPAYTRPFEEKLAEWMKLA
                     SYLSPSPSP"
     gene            970..2172
                     /gene="pcaF"
                     /locus_tag="VGB57_01050"
     CDS             970..2172
                     /gene="pcaF"
                     /locus_tag="VGB57_01050"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY0129960.1"
                     /translation="MTEAFICDAVRTPIGRYGGSLAHVRADDLAAIPIRALMERNPNL
                     DWDELDDVILGCANQAGEDNRNLARMAALLAGLPDKSGGVTLNRLCGSGMDAVTMAAR
                     VIRGSEADMVLAGGSESMSRAPFVMAKAQSAFDRNAEVYDTTIGWRFVNPKMKHAYGD
                     DTMPSTAENVAQEWQVSREDQDAFAVRSQDKAAAAQENGRFAAEIVPVSIPQRKGDPL
                     IVDRDEHPRKTSVEALARLKPIVRSDGTVTAGNASGVNDGAAAMVVASEEAARRNGLT
                     PRARIVGGAVAGVPPRIMGIGPAPASQKLLKCLGLSIGDMDVIEINEAFAAQALPVLR
                     ELGLPDDAAHVNPNGGAIALGHPLGMSGARLAMTATEELHRRGGRYALCAMCIGVGQG
                     IALVIERV"
BASE COUNT          372 a          804 c          726 g          342 t
ORIGIN      
        1 tgcttcggcc tcggcgcctg ggaattcccg gtcgccgctt attatcattc ggtccgactg
       61 aagcagcccg agcccctgcc ccccgcgggc cgaagcttcg ccgccctcgt gcggaccaac
      121 tacgtgaccc gcacgattcc gctcggcgag ccccaatatc tcttcctgga ggccatggcg
      181 gagggcggcg gcgtcgatga cggcctcgaa gcggcggcgc gcttcctcgc cacgacgccc
      241 gaaaaggtac ggcagtcctg gcattcgtcc ggagaccggc agcgctggat cgactggggc
      301 ttcttcatcg atggcgatca gacccgctag aagagttgcg gcgacggcac ctatccccga
      361 aaagcgcgca atggacgagg tcaagcgctc cttccctccc gtcgccgacg cccgcacccg
      421 agtgctcgtg ctcggcagcc tgcccggcga agagtcgctc gcccgaagcc aatattacgc
      481 caacccgcgc aaccatttct ggaggctgat cggagccgtg gtcggcgccg agctcgcccc
      541 cctcccctac gaggtccggc tcgaggccct gctcgaggcc cggatcggcc tttgggacac
      601 ggtcggttcg gcgacccggc gcggaagcct cgacggcgcc attcgcggcc acttggccaa
      661 cgacctcgcc gatctcgccg cccggctgcc cgagcttcgc gcggtcggct tcaacggcgg
      721 caagtcggcg tcgctcggaa tggcccagct ggcggggcgg cccgacctgg cgctgatccc
      781 gctgccgtcg agcagcccgg cctacacccg gcccttcgag gagaagcttg ccgagtggat
      841 gaagctcgcc tcttacttgt caccgtcgcc gtcaccctga acttgtttca gggtccagtt
      901 tcgagcatcg cagcggtcat caacctggat gctgaaacaa gttcagcatg acggacgacg
      961 gagcgagaca tgaccgaagc cttcatctgc gacgcggtgc ggaccccgat cggccgctac
     1021 ggcggaagcc tcgcccacgt ccgcgccgac gatctcgccg cgatcccgat ccgggcgctg
     1081 atggagcgca acccgaacct ggactgggac gagctcgacg acgtcatcct cggctgcgcc
     1141 aaccaggccg gcgaggacaa tcgcaacctc gcccggatgg cggccctgct cgccggcctc
     1201 ccggacaaat cgggcggagt gaccctcaac cgcctctgcg gctcgggcat ggacgccgtc
     1261 accatggccg ctcgggtgat ccgcggaagc gaggcggaca tggtcctcgc cggcggctcg
     1321 gagagcatga gccgcgctcc cttcgtcatg gccaaggccc agagcgcctt cgaccgcaac
     1381 gccgaggtgt acgacacgac gatcggctgg cgcttcgtca acccgaagat gaagcatgcc
     1441 tatggcgacg acaccatgcc gtccacggct gagaacgtgg ctcaggaatg gcaggtaagc
     1501 cgcgaggatc aggacgcttt cgctgtccgc agccaggaca aggccgccgc cgcgcaggag
     1561 aatggccgct tcgccgccga gatcgtcccg gtctccatcc ctcagcgcaa gggcgacccg
     1621 ctgatcgtcg accgcgatga gcatccgcgc aagaccagcg tcgaggcgct cgccagattg
     1681 aagccgatcg tccgctccga cggcaccgta accgccggca acgcgtcggg agtcaatgac
     1741 ggcgccgccg ccatggtggt ggcctcggag gaagcggcga ggcgcaacgg cctcaccccc
     1801 cgcgcccgga tcgtcggcgg cgccgtcgcc ggagtgccgc cccggatcat gggcatcggc
     1861 cccgctccgg cttctcagaa attgttgaag tgcctcggcc taagtatcgg ggatatggac
     1921 gttatcgaga tcaacgaagc cttcgccgcc caggcccttc cggtgctccg cgagctcggc
     1981 ctccccgacg acgcggccca cgtcaacccg aacggagggg ccatcgctct gggccacccg
     2041 ctcgggatgt cgggcgcccg cctcgccatg acggccaccg aggagctcca ccgccgaggc
     2101 ggccgctacg ccttgtgcgc catgtgcatc ggggtgggcc agggcatcgc tttggtcatc
     2161 gagcgggtgt aaaaagaggg acagtccctc ttttcgacgg cggggcctga aaaagaggga
     2221 ctgtccctct tttttgacgg cggg
//
LOCUS       DASWNU010000051         5045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_365016, whole genome shotgun sequence.
ACCESSION   DASWNU010000051 DASWNU010000000
VERSION     DASWNU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365016"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VGB57_01055"
     CDS             complement(<1..890)
                     /locus_tag="VGB57_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0129961.1"
                     /translation="MFFASKSDPGTHQLTARMQVTEVGVYRGPHYYSHTPMVRIQLDL
                     GRLEEWPTNRIAGFTEALLDLLPGVGRHGCSLRVRGGFERRLREGTWLGHVAEHIALE
                     LQTLAGSRATRGKTRSVKNRPGVYNVMFAYNEEKVGLMAGRVALELVHSLLPADLQGL
                     SGLDRIYPLDGEFELERRLDALKRMVRRTALGPSTKALVDEAERRGIPVMRLDEKSLL
                     QLGHGRHQQKIRASITGRTSLVAADLAGDKNMTKKLLDESGVPVPRGIVVRDAEEAVI
                     GARRLRFPLVTKPLDGNHGRG"
     gene            complement(1068..1955)
                     /locus_tag="VGB57_01060"
     CDS             complement(1068..1955)
                     /locus_tag="VGB57_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY0129962.1"
                     /translation="MLGPWAALRKARYALPQCVERVVPGPGPGAAARRCRKLNGAGLA
                     ATLGYFQSRQGRPEDIVAANVAATGLLADRVGDVYLSVKAPPLGFDTARLREIAHSAE
                     TADMALMFDAHAPADADRTLKAVSDLLGEFPRTGFALPARWLRSRGDAAAFRDSSARI
                     RLVKGEWADPSWRGQDVEASYLALAARLAGRAAPVAVATHHPELAERALRLLLEAGTP
                     CELEQLRGLPARRTMAVASRLGVPVRVYVPFGPGWWPYALDKALARPYLLSWMIRDRM
                     ERKAKTPPPSLPAKAGIQY"
     gene            complement(1955..3118)
                     /locus_tag="VGB57_01065"
     CDS             complement(1955..3118)
                     /locus_tag="VGB57_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0129963.1"
                     /translation="MIEGWVTAIETMPDLSAATAALARRPSARTAAMPDSSAMPFDLF
                     CEEEALVRSWRSLEAVASLPMQSHAFASALARTFLADSQVEVFFIREQGDIAALLTLC
                     REPGRFARWRMVGAHEVCEPDDALYRDPKGIRLLAEAIVGNGRALEVDRIPVGSALIP
                     ALRGALRGKGWMSVRPATPTPTIPLEPRWKDPASCFNSGRRSDFRRAARRAAEFGEVS
                     FEILSPGPEAFDALFDEAIGVEMRSWKREAGTAIAADRSKENCFRHYFRSACERGEFR
                     IAFMRIEGRAVAMQMALETLGRYWLFKIGFDEEFERCSPGTLLMLHTIGWAAERGLEA
                     YELLGNLEPWIAQFWTREQHDCVWVRAYPFNLKGAVAFAADACASLRKRLARP"
     gene            3194..>5045
                     /gene="acs"
                     /locus_tag="VGB57_01070"
     CDS             3194..>5045
                     /gene="acs"
                     /locus_tag="VGB57_01070"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0129964.1"
                     /translation="MDSRIPPPAALAGSSHADAETYETLYRQSLDDPDSFWAEQARRI
                     DWIEVPTRAGNWSFDPVDIKWYEDGILNLCHNCVDRHLEARADQVAILFEGDEPGEGR
                     SLTYGELYREVVRMANCLKALGAGKGDRVTLYMPMIPEAAVAMLACARIGAVHSVVFG
                     GFSPEALHGRIEDCASRFVITADGGRRGGRLVPLKANVDAALAKGAEVEAVLVVRHLG
                     NDIEWTEGRDHWLHDMAALVPDECPPEPMNAEDPLFILYTSGSTGKPKGVLHTTGGYA
                     VWTAATFHYVFDYKEGEIFWCTADVGWVTGHSYVVYGPLLNGATSLMFEGVPNHPDFS
                     RFWDVVERLKVDIFYTAPTAIRALMREGEGAVRKHDRSSIRLLGTVGEPINPEAWRWY
                     YEVVGESRCPIVDTWWQTETGGVMITTLPGAHDMKPGSAGRPFFGVEPQLVGPDGELL
                     VGAASGNLCIARSWPGQMRTVYGDHERFVQTYFSTYRGKYFTGDGCRRDEDNYYWITG
                     RVDDVLNISGHRLGTAEIESALVSHRCVAEAAVVGYPHDIKGQGIYCYVTLMEGEAES
                     PELEADLRRHVRTEISPIATPDIIHFTPGLPKTRSGKIMRRILRKIAENEF"
BASE COUNT          839 a         1735 c         1713 g          758 t
ORIGIN      
        1 ccgcggccgt gattgccgtc gagcggcttg gtcaccagcg ggaagcgaag ccgcctggcg
       61 ccgattaccg cctcctcggc gtcgcggacg acgattccgc gcggcaccgg cacgccgctc
      121 tcgtcgagca gcttcttggt catgttcttg tcgccggcga ggtccgcggc gaccagcgag
      181 gtgcggcccg ttatcgaggc ccggatcttc tgctggtggc ggccgtggcc aagctggaga
      241 aggctcttct cgtcaaggcg catcaccgga atcccgcgcc gctccgcctc gtcgaccagg
      301 gccttggtgc tcgggccgag cgccgtccgg cggaccatcc gcttcagcgc gtcgagccgc
      361 cgctcgagct cgaattcgcc gtccagcggg tagatccggt cgagtccgct caggccctgc
      421 aggtcggcgg ggaggaggga gtggaccagc tcgagcgcga cccggccggc catcagcccg
      481 accttctcct cgttataggc gaacatgacg ttgtagacgc cggggcggtt cttcaccgac
      541 cgggtcttgc cgcgcgtggc gcgcgagccg gcgagcgtct gaagctcgag cgcgatatgc
      601 tcggcgacat ggccgagcca ggtgccctcg cgaagccgcc gctcgaagcc gcccctcacg
      661 cgaagcgagc agccgtggcg gccgactccg ggaagaaggt cgagcaaggc ttccgtgaaa
      721 cccgcgattc ggttcgtcgg ccattcctcc agccgcccca gatcgagctg gatgcggacc
      781 atcggcgtat ggctgtaata atggggaccg cgatagactc cgacttcggt cacctgcatc
      841 cgcgcggtca gctggtgagt gccggggtcg ctcttcgaag cgaagaacat gccggttttg
      901 gagccatttt ctcgggtcgg cgcgtcggcc acgggcgcgg actggttcgg aggcgtcatg
      961 accgggttaa acccccggaa agagacccaa gttccggcag gatcgtcctc acctgggtta
     1021 atcccggcgc cggctggtgc tcccggcgaa gggccggggc ccggaaatca gtactggatc
     1081 ccggccttcg ccgggaggct gggaggaggc gttttcgcct ttctctccat cctgtccctg
     1141 atcatccagg acagcagata agggcgggcg agggccttgt ccaaggcata aggccaccag
     1201 ccgggtccga aggggacata gactcgaacg ggcacaccca ggcggctggc gacggccatg
     1261 gtccgcctcg cgggcagccc ccgcagctgc tccagctcgc acggcgtgcc ggcctcgagc
     1321 aggagcctga gggcgcgctc cgccagttcc gggtggtgag tcgcgacggc gaccggagcg
     1381 gcgcgcccgg cgaggcgggc ggcgagggcg aggtagctcg cctcgacgtc ctggcctctc
     1441 cagctgggat cggcccattc gcctttgacc agccggatcc gcgcggagct gtcccggaag
     1501 gcggcggcgt ccccccggct tcgcagccag cgggcgggca aagcgaagcc cgtcctggga
     1561 aattcgccca gcaggtccga aaccgcctta agcgtgcgat ccgcatccgc gggggcgtgg
     1621 gcgtcgaaca tcagggccat atcggccgtc tccgccgagt gggcgatttc gcgcaggcgg
     1681 gcggtgtcga accccagggg aggggccttt accgacagat agacgtcgcc gacgcgatcg
     1741 gcgaggagcc ccgtcgcggc gacgttggcg gcgacgatgt cctccggccg gccctggcgc
     1801 gactggaaat aacccagggt cgcggccagc ccggcgccat tgagcttgcg gcagcgccgg
     1861 gcggcggcgc ccggacccgg tcccgggacg acccgctcga cacattgggg aagagcgtag
     1921 cgcgccttcc tgagcgcggc ccagggcccg agcatcaggg ccgagccagc cgcttgcgca
     1981 gcgaagcgca ggcgtccgcg gcgaaagcga ccgcgccctt caggttgaac gggtaagcgc
     2041 gcacccagac gcaatcatgc tgctcgcggg tccagaattg cgcgatccag ggctcgaggt
     2101 tgccgagcag ctcataggct tcgagcccgc gctccgccgc ccagccgatc gtatggagca
     2161 tgagcaaggt gcccggcgag cagcgttcga actcctcgtc gaagccgatc ttgaacagcc
     2221 agtagcgccc caacgtctcg agcgccatct gcatggcgac ggcgcgtccc tcgatccgca
     2281 tgaaggcgat ccggaactcg ccccgttcgc aggccgagcg gaaataatgg cggaagcaat
     2341 tctccttgga ccggtcggcg gcgatcgcgg ttcccgcctc gcgcttccag ctgcgcatct
     2401 cgactccgat cgcctcgtcg aacagggcgt cgaacgcttc cggaccgggc gacaatatct
     2461 cgaacgagac ctcaccgaac tcggccgccc gtcgcgccgc ccggcggaaa tcggagcggc
     2521 gcccggaatt gaagcaggag gcaggatcct tccagcgcgg ctccagcggg atggtcggag
     2581 tgggagttgc cggtcggacc gacatccagc ccttgccgcg caaggcgccg cgaagggccg
     2641 ggatcagcgc cgatcccacc gggatccggt ccacctccag ggcacgcccg ttgccgacga
     2701 tcgcctcggc gagaagcctg atgcctttcg gatcgcggta gagagcatcg tccggctcgc
     2761 acacctcatg cgctcccacc attcgccagc gggcgaagcg gccgggctcg cggcacaagg
     2821 tcagcaaggc ggcgatgtcg ccctgctcgc ggatgaagaa cacctcgact tgcgagtcgg
     2881 cgagaaaggt tcgggcgagc gccgaggcga aggcatggct ctgcatcgga agcgaagcga
     2941 cggcctcgag gcttcgccac gagcggacca gcgcctcctc ctcgcagaaa aggtcgaaag
     3001 gcatggccga ggaatcgggc atcgccgccg tccgggcgct cgggcgccgg gcgagcgcgg
     3061 ccgtggccgc ggacaaatcc ggcatcgtct cgatggctgt gacccaccct tcgatcatcg
     3121 aaggggcttt aacccaggtg cgtaaacatt ttgcgtcttt gccgacggca ccctgcaggc
     3181 ttagggacgg cgcatggaca gccgcatccc gccccccgcc gctctggccg gcagcagcca
     3241 tgccgacgcc gaaacctacg agacgctcta ccggcagtcg ctcgacgatc cggacagctt
     3301 ctgggcggag caggcgcggc ggatcgattg gatcgaggtt ccgactcgcg ccggcaactg
     3361 gtccttcgat ccggtcgaca tcaaatggta tgaggacggg atcctcaacc tctgccacaa
     3421 ttgcgtcgac cgccatctcg aagcgcgcgc cgaccaggtc gcgatcctgt tcgaagggga
     3481 cgagcccggc gagggccgga gcctcactta cggcgagctc taccgcgagg tcgtccggat
     3541 ggcgaactgc ctgaaggctt tgggcgccgg caagggcgac cgggtcacgc tctacatgcc
     3601 gatgatcccg gaagccgcgg tggcgatgct cgcctgcgcc cgaatcggag cggtccactc
     3661 ggtcgtcttc ggcggcttct cccccgaggc gcttcacggc cggatcgagg attgcgccag
     3721 ccgcttcgtg atcaccgccg acggcggcag gcgcggcggc cggctcgtgc ccctgaaggc
     3781 caatgtcgac gccgctttgg cgaagggggc ggaggtggag gccgtgctcg tcgtgcgcca
     3841 cctcggcaac gacatcgagt ggaccgaagg ccgcgaccat tggcttcacg acatggcggc
     3901 cctcgtcccg gacgaatgcc cgccggagcc gatgaacgcc gaggacccgc tcttcatcct
     3961 ctacacgtcg ggctcgaccg gaaagcccaa gggcgtcctc cacaccaccg gcggctacgc
     4021 cgtctggacc gcggcgacct tccattacgt cttcgactat aaggagggcg agatcttctg
     4081 gtgcaccgcc gatgtcggct gggtcaccgg ccatagctac gtcgtctacg gcccgctcct
     4141 caacggcgcc accagcctga tgttcgaggg cgttcccaac catcccgatt tcagccgctt
     4201 ctgggacgtg gtcgagcggc tcaaggtcga catcttctac accgccccga ccgccatccg
     4261 ggcgctgatg cgcgaagggg agggggcggt ccgaaagcac gaccgctcgt cgatccggct
     4321 gctcggaacg gtcggcgagc cgatcaaccc ggaagcctgg cgctggtatt acgaagtcgt
     4381 cggcgagagc cgctgcccga tcgtcgacac ctggtggcag accgagaccg gcggggtgat
     4441 gatcaccacc ttgccgggcg cccacgacat gaaaccgggc agcgccggcc ggcccttctt
     4501 cggggtcgag ccgcagctgg tcggccccga cggcgagctt ctggttggcg cggcctccgg
     4561 gaacctctgc atcgcccgct cctggccggg gcagatgcgc acggtctacg gggaccacga
     4621 gcgcttcgtt cagacctatt tctcgaccta ccgcggcaaa tatttcacag gcgacggctg
     4681 ccgccgcgac gaggacaatt attactggat cacgggacgg gtggacgacg tcctcaacat
     4741 ctccggccac cgactcggca ccgccgagat cgaaagcgct ctcgtctccc accgctgcgt
     4801 cgccgaagcc gcggtggtcg gctatccgca cgacatcaag gggcagggca tctattgcta
     4861 cgtcacgctg atggaaggcg aggccgaaag ccccgagctc gaggccgacc tgcgccgcca
     4921 cgtccggacc gagatcagcc cgatcgcgac gcccgacatc atccacttca cgcccggcct
     4981 gcccaagacg cgctcgggca agatcatgcg ccgaatcctg aggaagatcg cggagaacga
     5041 gttcg
//
LOCUS       DASWNU010000052         6366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_390689, whole genome shotgun sequence.
ACCESSION   DASWNU010000052 DASWNU010000000
VERSION     DASWNU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390689"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGB57_01075"
     CDS             <1..400
                     /locus_tag="VGB57_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129965.1"
                     /translation="GLAMAEADRYLPDILTRIEAILAANGLTDEPITFRISGCPNGCS
                     RPYVAEIALTGRAPGKYNLYLGGGFHGERLARMVRENIGETEILRVLEDLLGRYASER
                     QHGERLGDFVERISYLASEQQIPGRIDPII"
     gene            492..1517
                     /locus_tag="VGB57_01080"
     CDS             492..1517
                     /locus_tag="VGB57_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0129966.1"
                     /translation="MKPVRAFILAAGLAGALALSGCGEGPKQKSGELLNVSYDPTREL
                     YRDVNESFARKWKAETGQDLEIRQSHGGSGKQARSVIDGLAADVVTLALAYDIDEIAQ
                     RARSLPANWQTRLPDNSSPYTSTIVFLVRKGNPKRIVDWGDLVRPDVKVITPNPKTSG
                     GARWNYLAAWGYADRRFGSPARTREYMTRLFRNVPVLDSGARGSTTTFVERAQGDVLL
                     SWENEAFLAVNKLGKGQFEIVVPSLSILAEPPVALVDKVVDQKGTRPAAEAYLKYLYT
                     PEAQELIAKHYYRPRDPAVAARHKAQFPDVAMLTIDKDFGGWAKAQKTHFADGGEFDR
                     IFKKAKR"
     gene            1579..3993
                     /locus_tag="VGB57_01085"
     CDS             1579..3993
                     /locus_tag="VGB57_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0129967.1"
                     /translation="MRFLLLASAALATSPAYAQTPSESVPPETEAPPAEAAPGPSGDD
                     IVVTARRRDEQLQDVPLAISVLGGAQLDATGSINVSRLQQIQPSVQFFSTNPRNSAAN
                     IRGLGAPFGLTNDGIEQGVGIYIDEVYYSRIAVATFDFLDVERIEVLRGPQGTLYGKN
                     TTAGAINIVTRAPSFEPEARLELSLGNLDYIAAKASVSGPIVEDKLAVRLAASKSRRR
                     GTIFNLATDRWVNEQDNLGLRGQLLWRASGALDVTLSADYNRQNPECCAQIYVRTGAT
                     QRPLSRQYAALASAFGYAPPSTDPFDRLTDLDTPLDAFQELGGVALRAKWDLGPGTLT
                     SVTAWRFWNWGPSNDRDFTGLPITTVSANPSRQNQYSQELRYAGSSGNLDFVGGLFAY
                     RQEQHTTGAQEQGPAASRWLLNPTSANANNPAVLNGLRSENNIRLETTSLAAFGQLSW
                     RVTDRLRLQPGLRFNYDKKIGSYVAVVTTGTGSTSLNNDQRGILAPQSYEAKFSDWNI
                     SGDFTAAYDIAPDVLAYATYAKSFKSGGINLAGLPLDATNNPILGAATVKPEKVDHFE
                     VGLKTQFLNRRATLNLAAFWTNIKDYQATVTNGQLGVLRGYLANADKVRVRGVEADFS
                     VRPMRGLSLYASAAFTDHEYVRFVDAPCPPELAGGGAATPANPASPPGTPGGFSPANC
                     DISGQWLPGISKWAFSFGGEYSLPAKLLGRSGQVYAGADGSYRSRFSSNPSRSAYTDI
                     DGYGLASFRLGFRTDDGWNSFLWIRNAFDSEYYDVLATQSGSTGLIVGQPGDPRTWGV
                     TIQKRF"
     gene            4076..5473
                     /locus_tag="VGB57_01090"
     CDS             4076..5473
                     /locus_tag="VGB57_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0129968.1"
                     /translation="MRFLLLTGTALIALAGSPAYAQEPAPAPTGDPVIDRLNALEARV
                     RQLEARNAELEQQAAATTATRVEAAEKREAKQVQFGWAPTLSDEKGDFTFKPRGVIDA
                     DYGIFNERAGGYDYNDGTAFRRARIGLEGTAMKVFNYRIEVDFAGNQVGITDAYLQYA
                     GIEDLLITAGQHKAPFGLESNNSDNYNIFLERGMFTNAFGNAGAERRIGLSAAWAAKE
                     NLNLAAGLFGDNESITRSTGAAVTDSPDESLGANARIAWEPVFEKGRILHLGVSGYYR
                     TGLKSGDVADAVRLSDRPNIRIDNGNIADSGVVSGVDSIRYAGAEAAAVFGPFTLAGE
                     YGRLWLDRPGLADPTFDGFYVYATWFPTGESRPFKNGNFDRVKPRRNFASGGAGAFEL
                     ALRYDRIDLSDTPVLARAGNRAESLTLGLNWYLNPNAKLLFNWIRFNGTNTPLDPIGT
                     RTAGDALATRLHVDF"
     gene            5719..>6366
                     /locus_tag="VGB57_01095"
     CDS             5719..>6366
                     /locus_tag="VGB57_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0129969.1"
                     /translation="MGKIGVPEAPLSEWTDVPAVGVEMHSRYWPGTGERGAAPILLLP
                     GLALSSRSLFPLARRLSARGYEVHALDLPGFGRTRKPPKATWPAGPNVRAQADQLRDW
                     MDSRGLGRVVLYGISVGSQVAADFAVRYPERVERLVLAGPAPDPAMRTPLRQYGGVLM
                     NMPFEAPSLNDVFQVEYASAGLARMVQQLRRTVDDAIETKLPGIAVPALVVRGQFD"
BASE COUNT         1136 a         2296 c         1945 g          989 t
ORIGIN      
        1 cggtctcgcc atggccgagg ccgaccgcta ccttcccgac atcctgaccc ggatcgaagc
       61 gatcctcgcc gccaacggcc tcaccgacga gccgatcacc ttccggatca gcggctgccc
      121 caacggttgc tcccggccct atgtcgccga gatcgccctg accggccggg cgccgggcaa
      181 gtacaatctc tacctcggcg gcggctttca cggcgagcgg ctcgcgcgaa tggtccggga
      241 gaatatcggc gaaaccgaga tactgagggt ccttgaagac ctgctcggcc gttacgcgag
      301 cgagcggcaa cacggcgaac ggctcggcga tttcgtcgag cgaatctcct acctggcgag
      361 cgagcagcaa atcccggggc ggatcgaccc gataatctga ggaggaaaat tcgcacaggc
      421 ggcaagcgcc gcccgggaaa gtgccgattg attgtctact aaattaatta agtaataaga
      481 gggataacgc catgaaaccc gttcgagcct tcatccttgc cgccggcctg gccggcgccc
      541 ttgccctctc cggctgcgga gagggtccga agcagaaaag cggagagctc ctcaacgtct
      601 cctacgaccc gacccgggag ctctaccgcg acgtgaacga gagcttcgcc cgcaagtgga
      661 aggccgagac cggccaggac ctcgagatcc ggcagagcca cggcggttcc ggcaagcagg
      721 cccgttccgt gatcgacggc ctcgccgccg acgtcgtcac cctggcgctc gcctacgaca
      781 tcgacgagat cgcccagcgt gccagatcgc tcccggcgaa ctggcagacc cggctgccgg
      841 acaacagcag cccctacacg tcgacgatcg tcttcctcgt ccgcaagggc aatccaaagc
      901 ggatcgtcga ctggggcgat ctcgtccgcc ccgacgtcaa ggtcatcacc cccaatccca
      961 agaccagcgg cggcgcccgc tggaactacc tcgccgcctg gggctatgcc gaccgcaggt
     1021 tcggctcccc cgcccgcacc cgcgaatata tgacccgcct gttccgcaac gtgcccgtgc
     1081 tcgacagcgg cgcgcggggc tccaccacca ccttcgtcga gcgcgcccag ggcgatgttc
     1141 tcctctcctg ggagaatgag gccttcctcg cggtcaacaa gctcggcaag ggccagttcg
     1201 agatcgtcgt cccctcgctc tcgattctcg ccgagccgcc ggtggccctc gtcgacaagg
     1261 tcgtcgacca gaaaggcacg aggccggcgg ccgaggccta tctcaaatat ctctacacgc
     1321 ccgaggccca ggagctgatc gcgaagcatt attaccggcc gcgcgatcct gcggtcgccg
     1381 cccgccacaa ggcgcaattc cccgacgtcg cgatgctgac catcgacaag gatttcggcg
     1441 gctgggccaa ggctcagaag acccacttcg ccgacggcgg cgaattcgac cggatattca
     1501 agaaagcgaa gcgctgaagg tcgcgcgctg actgggcccc ggtcgcccgg ggctctcttt
     1561 attcgaagga gaacatcgat gcgtttcctg ctcctggcca gcgccgcgct ggccacatcg
     1621 cccgcttacg cccagacgcc ctcggagtcc gtgccgcccg agaccgaagc cccgccggcg
     1681 gaggcggccc cggggccgtc gggcgacgac atcgtcgtca ccgccaggcg ccgcgacgaa
     1741 cagctccagg acgtcccgct cgccatctcc gtgttgggcg gagcgcagct ggacgcgacc
     1801 ggaagcatca atgtcagccg gcttcagcag atccagcctt cggtgcagtt cttcagcacc
     1861 aacccgcgca actcggcggc caacatccgc ggcctgggcg cgccgttcgg cctgaccaac
     1921 gacggcatcg agcagggcgt cgggatctat atcgacgagg tctattacag ccgtatcgcg
     1981 gtcgcgacct tcgacttcct cgacgtcgag cggatcgagg tgcttcgcgg cccgcagggg
     2041 acgctgtacg gcaagaacac caccgccggg gcaatcaaca tcgtcacccg cgcgcccagc
     2101 ttcgagccgg aggctcggct cgagctgtcg ctcggcaatc tcgactatat cgccgccaag
     2161 gcctcggtgt ccggtccgat cgtcgaggac aagcttgccg ttcgcctcgc cgcctcgaag
     2221 agccggcggc gcggcaccat cttcaaccta gccaccgacc gctgggtcaa cgagcaggac
     2281 aatctcggcc tgcgcggcca gttgctctgg cgggcgagcg gggcgctcga cgtgaccctt
     2341 tcggccgatt acaaccggca gaacccggaa tgctgcgccc agatctacgt ccgcaccggg
     2401 gcgacccagc gaccgctcag ccgccaatat gccgcgctgg cctcggcctt cggctacgcc
     2461 cctccgagca ccgacccctt cgaccgcctc accgatctcg acaccccgct cgacgccttc
     2521 caggagcttg gcggagtcgc gcttcgggcg aaatgggacc tgggcccggg gacgctcacc
     2581 tccgtcaccg catggcgctt ctggaactgg ggcccgtcga acgaccgcga cttcaccggc
     2641 cttccgatca ccaccgtctc cgccaatccc tcgcggcaga accaatattc gcaggagctt
     2701 cgctatgcgg gatcgagcgg caatctcgat ttcgtcggcg ggctgttcgc ctaccggcag
     2761 gagcagcaca cgaccggagc ccaggagcaa gggccggcag ccagccgctg gctgctcaac
     2821 ccgacgagcg ccaacgccaa caacccggca gtgctgaacg ggcttcgttc ggaaaacaac
     2881 atccggctcg agacgaccag cctcgccgcc ttcggccagc tcagctggcg ggtgaccgac
     2941 cggcttcgcc tccagccggg cctgcggttc aattacgaca agaagatcgg ctcatatgtg
     3001 gcggtcgtga ccaccggcac cggaagcacg agtctgaaca acgaccagcg cggaatcctg
     3061 gcgccgcaaa gctacgaggc gaaattcagc gactggaaca tctccggcga tttcaccgcc
     3121 gcttacgaca tcgccccgga tgtgctggcc tacgcgacct acgccaagag cttcaaatcg
     3181 ggcgggatca acctggccgg ccttccgctc gacgcgacga acaacccgat actcggagcg
     3241 gcgacggtga agcccgaaaa ggtcgaccat ttcgaggtcg gcctcaagac gcaattcctg
     3301 aaccggcggg cgactctgaa cctcgccgcc ttctggacga acatcaaaga ctatcaggcg
     3361 accgtcacca acggccagct cggcgtcctg cgcggctatc tggccaacgc agacaaggtg
     3421 agggtgcggg gtgtcgaagc cgatttctcg gtgcggccca tgcgggggct cagcctctac
     3481 gccagcgcgg ccttcaccga ccacgaatat gtgcgcttcg tcgacgcccc ctgcccgccc
     3541 gagcttgccg gcggcggcgc cgcgacgccc gccaacccgg ccagccctcc gggaacgccc
     3601 ggcggcttca gcccggccaa ttgcgacatt tccggccagt ggctgccggg catctcgaaa
     3661 tgggccttct ccttcggcgg ggagtacagc cttccggcga agcttctcgg ccgctccggc
     3721 caggtctatg ccggggccga cggaagctac aggtcgcgct tctcctccaa cccctcccgg
     3781 tcggcctata cggatatcga cggctatggg ctcgcgagct tccggctcgg cttccgcacc
     3841 gacgacggct ggaacagctt cctgtggatc cgcaacgcct tcgactccga atattacgac
     3901 gtgctggcga cccaatcggg aagcaccggc ctcatcgtgg gtcagcccgg cgatccacgc
     3961 acctggggtg tcacaattca gaaaagattc tgaaggaaaa gcgaaacgta gaggcttacc
     4021 ggcttgactg cgcttccggc ggtgccgaac gcctcctctg tcaacggagg accatatgcg
     4081 tttcctgctc ctgaccggca ccgccctgat cgcgctcgcc ggcagccccg catacgccca
     4141 ggagccggcc ccggcgccga ccggcgaccc ggtcatcgac cgcctcaacg cgctcgaggc
     4201 gcgggtgcgc cagctggaag cccgcaacgc cgagctcgag cagcaggccg ccgccaccac
     4261 cgcgacccgg gtcgaagccg ccgagaagcg cgaggcgaag caggtccagt tcggctgggc
     4321 cccgaccctt tccgatgaga aaggcgattt caccttcaag ccgcgcggag tcatcgacgc
     4381 cgattacggg atcttcaacg aacgggcggg cggctacgat tataatgacg gaaccgcctt
     4441 ccgccgcgcc cggatcggcc tcgaaggcac ggcgatgaag gtgttcaact acaggatcga
     4501 ggtggatttc gccggcaatc aggtcgggat caccgacgcc tatctccaat atgcagggat
     4561 cgaggacctg ctgatcaccg caggccagca caaggcgccc ttcggcctcg aatccaacaa
     4621 cagcgacaat tacaatatct tcctcgagcg cggcatgttc accaacgcct tcggcaatgc
     4681 cggcgccgag cgccgcatcg gcctctccgc cgcctgggcc gcgaaggaga atctcaacct
     4741 cgcggccggc ctgttcggag ataatgagag catcacccgc tcgaccgggg ccgccgtgac
     4801 cgactcgccc gacgagagcc tcggcgcaaa tgcccggatc gcctgggagc cggtcttcga
     4861 gaagggcaga atcctccacc tgggcgtgtc cggctattac cgcaccggcc tcaagtcggg
     4921 cgacgtcgcc gacgccgtcc ggctcagcga ccgccccaac atccgcatcg acaacggcaa
     4981 tatcgccgat tccggggtgg tgagcggagt cgactccatc cgctacgcgg gcgcggaggc
     5041 cgccgccgtc ttcgggccct tcaccctcgc tggggaatat ggccgcctct ggctcgaccg
     5101 gcccggcctc gccgatccga ccttcgacgg cttctacgtc tacgccacct ggttcccgac
     5161 cggcgagtcg aggccgttca agaacggcaa tttcgaccgg gtgaagccgc gcaggaattt
     5221 cgcgagcggc ggcgccggag ccttcgagct cgccctgcgc tacgaccgga tcgacctctc
     5281 cgacaccccc gttctcgccc gcgccggcaa ccgcgccgaa agccttaccc tcggcctcaa
     5341 ctggtacctc aaccccaacg ccaagctgct gttcaactgg atccgcttca acggcaccaa
     5401 cacccccctc gaccccatcg gcacccgcac cgcaggcgac gccctggcga cccgcctgca
     5461 cgtcgatttc tagacgcaag tgcgggcatc ccggcccggc cgtacgctgc ttcccgtacg
     5521 gccgggtgct gggcgctcac ctgcctcggt gccgatttaa cctgagtttt tgatctacag
     5581 aatcaaagac ttggcctaag cgcgggtgag cggctgagcg ccccattaca gggccgggcc
     5641 gctggaacgc ccgcctcccc ttgccgttcg gccttgcctc agcagtcggt tcgacaggag
     5701 catctggcaa tgaatgtgat ggggaagatc ggcgtcccgg aagcgccgtt aagcgagtgg
     5761 accgacgttc cagcggtcgg agtcgagatg cacagccgtt actggcccgg cacgggcgag
     5821 cgcggcgcag cgcccatcct cctgctgccg gggctcgcct tgtcgagccg aagcctgttc
     5881 ccgctcgcgc gacggctttc cgcccggggc tacgaggtgc atgccctcga tcttcccggc
     5941 ttcggccgaa cgcgcaagcc cccgaaggcc acctggccgg ccggcccgaa cgtacgggcg
     6001 caggccgatc agctgcgcga ctggatggat tcccgcggac tcgggcgagt ggtgctctac
     6061 ggcatctcgg tcggctcgca ggtcgccgcc gatttcgccg tgcgttatcc cgaacgggtc
     6121 gagcggctgg tgcttgcggg cccggcgccc gatccggcga tgcgcacccc gcttcggcaa
     6181 tatggcggag tgctcatgaa catgccgttc gaagcgccgt ccctgaacga cgtgttccag
     6241 gtcgaatatg cgtcggcggg cctggcgcgc atggttcagc agctgcgccg caccgtcgac
     6301 gacgcgatcg agacgaaact tccgggcatc gcggtccccg ctctcgtggt tcggggccag
     6361 ttcgat
//
LOCUS       DASWNU010000053         3800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_469570, whole genome shotgun sequence.
ACCESSION   DASWNU010000053 DASWNU010000000
VERSION     DASWNU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469570"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VGB57_01100"
     CDS             complement(<1..407)
                     /locus_tag="VGB57_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY0129970.1"
                     /translation="MSDESDPLAAALSAVTEARGRIAAPRLRDGVASLVLDVGGLATQ
                     ARAGLERQIRAALVGVPGVREVRIAMTADKVERAIIAVGSGKGGVGKSTVAANLAVAL
                     ARAGRRVGLVDADIYGPSLPRIMGNSDRPELNE"
     gene            478..1560
                     /gene="hflK"
                     /locus_tag="VGB57_01105"
     CDS             478..1560
                     /gene="hflK"
                     /locus_tag="VGB57_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY0129971.1"
                     /translation="MARFFGWGARLGAVLNENRGGPWGPSGDGGSGDGGGGGGPRNPW
                     GQPPRKRRTPGAGTNVASFDDFLRRSREKFGGRFPHQEGRPYWLYGLGVFVLLWLLFT
                     CFHLVGPQERGVVTLFGKYSRTVNSGIAMTLPLPIERLQKVDVGQIRTRDIGSPDASQ
                     ENLILTGDQNIIDVAYSVRWTIRDPQLYLFQINDPESTIGEVAESSMRAVLATVSLDD
                     AIGAGRSDIESRVSLVMQQVLDRYGAGVRIEGIAIKQSDPPATVNNAFKEVSAAQQEA
                     QSYINQARAYAQQQTARAQGDAAAFDKVYAEYKLAPEVTRRRMYYETMERVLSKVDKT
                     VVEANGVTPYLPLPQIQRQAQEGAAR"
     gene            1557..2414
                     /locus_tag="VGB57_01110"
     CDS             1557..2414
                     /locus_tag="VGB57_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY0129972.1"
                     /translation="MSRALRNPIAIAFLAILLLVLVGSTFAIVPETKQAVIVRFGKPQ
                     EKPINAYQRGVEFGNTGAGLIARWPFIDNIVWIDKRILSVKMDRQQVLSTDQLRLQVD
                     AFARYRIVDPLQMYVSARTEERVSDALKPILGSAIRNELGKRQFAALLSPERGQLMAN
                     IKNALNRVARQYGAEVVDVRIKRADLPEGTPLDSAYERMRTARQQEAGAIRAQGYKQA
                     QIIKADADAEAAKTYAEAYGKDPDFYDFYRAMQSYETTFAPGENQGRSSIILSPDNQY
                     LQEFKGRAR"
     gene            2555..>3800
                     /locus_tag="VGB57_01115"
     CDS             2555..>3800
                     /locus_tag="VGB57_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0129973.1"
                     /translation="MRYVYGISAAILLGGAAATLTVGPVGAQTAQNDPGTMASAAPRP
                     GAPMSFADLAARLQPAVVNISTKQSIQINRSRQVPPGFEDFFRQFGVPVPEGGGEAPI
                     TQRGGSLGSGFIISADGYVVTNNHVVSPARTGATVEQITVTLSDRREYEATLVGRDAA
                     SDLALLKIKGNNLPFVRFGDSKQVRVGDWVVAIGNPFSLGGTVTAGIVSALHRNIGAG
                     TYDRYIQTDASINMGNSGGPMFDLNGNVIGINTALISPTGGNVGIGFAIPAEQAFPII
                     DSLRKGQRPQRGYIGVSLQALDEDIAASLGIPKNQGELIRGVTAGGPAARAGVQQGDV
                     VVSVGGQPVTPDESLAYLVSRQPIGSRVPIEIVRDGRRQTVNVAIGERPTEEELARIN
                     GIEDETPVREPAEAQQSTSQRSA"
BASE COUNT          651 a         1350 c         1232 g          567 t
ORIGIN      
        1 ttctcgttga gctcggggcg gtcggaattg cccatgatcc ggggcaggga cggcccgtag
       61 atgtcggcgt cgaccaggcc gacccggcgg cccgcccggg ccagcgcgac ggcgaggttc
      121 gccgccacgg tggacttgcc gaccccgccc ttgccgcttc cgacggcgat gatggcgcgc
      181 tccaccttgt ccgcggtcat cgcgatccgg acctcgcgga cccccggcac gcccaccaga
      241 gcggcgcgga tctgccgctc gagaccggcg cgcgcctgcg tggcgagccc accgacgtcg
      301 agcaccaggc tggccacgcc gtccctgagc cgcggcgcgg cgatccggcc tctggcctcc
      361 gtcacggcgg aaagggcggc agctagcggg tcggactcgt cgctcatccg gcgcagatag
      421 ggggtgctgt tgggcttgcc actgtttttc cgcccgagcg ctacctataa aggctccatg
      481 gcaagatttt tcgggtgggg cgcgcgtctc ggcgccgtgt tgaatgaaaa tcggggcggg
      541 ccctgggggc cctctggcga cggcggctcc ggcgacggcg gcggcggcgg cggtccgcgc
      601 aatccctggg ggcagccccc gcgcaagcgg cgcacccccg gcgccggcac caacgtcgcc
      661 tcgttcgacg acttcctcag gcggagccgc gagaagttcg gcggccgctt cccccatcag
      721 gaagggcgcc cctactggct ctatggcctg ggcgtcttcg tgcttctgtg gctgctgttc
      781 acctgcttcc acctcgtcgg tccgcaggag agaggcgtcg tcaccttgtt cggcaaatat
      841 agccgcacgg tgaattcggg catcgccatg accctgcctc tgccgatcga gcgtctccag
      901 aaggtcgacg taggccagat ccgcacccgc gatatcggtt cgcccgacgc atcgcaggag
      961 aatctgatcc tcaccggcga ccagaacatc atcgacgtcg cttattcggt gcgctggacg
     1021 atccgcgatc cgcagctcta cctcttccag atcaacgatc ccgaatcgac gatcggcgaa
     1081 gtcgccgaaa gctcgatgcg ggccgttctc gcgaccgtct cgctcgacga cgcgatcggc
     1141 gcaggccgga gcgacatcga gtctcgcgtg tcgctggtca tgcagcaggt gctcgaccgc
     1201 tacggggccg gagtccggat cgagggtatc gcgatcaagc agtcggatcc gccggcgacg
     1261 gtcaacaacg cgttcaagga agtgtcggcg gcacagcagg aggcgcagtc ctacatcaac
     1321 caggcgcgcg cctatgccca gcagcagacc gcgcgggccc agggcgacgc cgcggctttt
     1381 gacaaggtct acgccgagta caagctcgcc cctgaagtga cccggcggcg gatgtattac
     1441 gagacgatgg agcgggttct ctccaaggtc gacaagaccg tggtcgaggc caatggggtg
     1501 actccctatc tgccgcttcc ccagatccag cgccaggccc aggaaggagc cgcccgatga
     1561 gccgcgcgct tcgcaaccca atcgccatcg ccttcctggc gatcctgctt ctggtcctcg
     1621 tcggaagcac cttcgcgatc gtgccggaga cgaagcaggc ggtgatcgtc cgcttcggca
     1681 agccgcagga gaagccgatc aacgcctacc agcgaggcgt cgagttcggc aacacgggcg
     1741 cgggcctcat cgcccgctgg ccgttcatcg acaacatcgt ctggatcgac aagcgcatcc
     1801 tcagcgtcaa gatggaccgc caacaggtgc tctcgaccga ccagctcagg ctccaggtcg
     1861 acgccttcgc ccgctaccgg atcgtcgacc cgctgcagat gtacgtctcg gccaggaccg
     1921 aggagcgggt cagcgacgcg ctcaagccga tcctcggctc ggcaatccgc aacgagctcg
     1981 gcaagcgcca gttcgcggcg ctgctcagcc ccgagcgcgg gcagctgatg gccaacatca
     2041 agaacgcgct caaccgggtc gcgcgccaat atggggccga agtcgtcgac gtccggatca
     2101 agcgcgccga cctgcccgag ggcacgccgc tcgactcggc ctatgagcgg atgcggaccg
     2161 cccgccagca ggaggccggg gcgatccggg cccagggcta caagcaggcg cagatcatca
     2221 aggccgacgc ggacgcggag gcggcgaaga cctatgccga ggcctatggc aaggatcccg
     2281 acttctacga cttctaccgg gcgatgcaga gctacgagac gaccttcgcc cccggcgaga
     2341 accagggacg ctcgagcatc atcctgtcgc cggacaacca gtatctgcag gagttcaagg
     2401 gccgggcccg atagcggccg caaactcggc aatcgctgca cgtcgttacg cggccgctat
     2461 tcaaatccgg ttcagccgca ccagatacgt ttgtaatcga taagacagcc cggatctgcc
     2521 cgcccgggtc gaagcaaatg aggagactgc aatcgtgcgt tacgtctatg gcatctcggc
     2581 cgccattctt ctgggcggcg ccgccgccac gctgacggtg ggccccgtcg gtgcccagac
     2641 cgcccagaac gatcccggca ccatggcgtc ggccgctccg cgccccggcg cgccgatgag
     2701 cttcgccgac ctcgccgccc ggctccagcc ggcggtggtc aacatctcga ccaaacagtc
     2761 gatccagatc aaccggagcc gacaggtccc ccccggcttc gaggacttct tccgccagtt
     2821 cggggttccc gtccccgagg gcggcggcga agcgccgatc acccagcgcg gcggctcgct
     2881 cggctccgga ttcatcatct cggccgacgg ctacgtcgtc accaacaacc acgtcgtgtc
     2941 gccggcccgc accggcgcca ccgtcgagca gatcacggtg accctgtccg atcgccgcga
     3001 atatgaagcc accctggtcg gccgcgacgc cgcctccgac ctcgccttgc tgaagatcaa
     3061 ggggaacaat ctgcctttcg tccgcttcgg cgattccaag caggtgcggg tcggcgactg
     3121 ggtcgtggcg atcggcaatc cgttcagcct cggcggcacc gtcaccgccg gaatcgtctc
     3181 ggcgctgcac cgcaacatcg gcgccggcac ctacgaccgc tacatccaga ccgacgcaag
     3241 catcaacatg ggcaattcgg gcgggccgat gttcgacctg aacggcaacg tcatcggaat
     3301 caacacggcg ctcatttcgc cgaccggcgg caatgtcggc ataggcttcg cgatcccggc
     3361 cgagcaggcc ttcccgatca tcgactcgct gcgcaagggc cagcggccgc agcgcggcta
     3421 tatcggggtc agcctgcagg cgctggacga ggatatcgcc gcctcgctcg ggatccccaa
     3481 gaaccagggc gagctgatcc gcggagtcac cgccggcggg ccggcggccc gcgccggagt
     3541 gcagcagggc gacgtcgtcg tcagcgtcgg cgggcagccg gtcactccgg acgaatcgct
     3601 cgcctacctc gtctcgaggc agccgatcgg ctccagggtt ccgatcgaga tcgtccgcga
     3661 cggccggcgg cagacggtca acgtcgccat cggcgagcgc ccgaccgagg aggagcttgc
     3721 ccgcatcaac gggatcgagg acgagacccc ggtccgcgag cccgccgaag cccagcagtc
     3781 gaccagccag cgctcggcgc
//
LOCUS       DASWNU010000054         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_235689, whole genome shotgun sequence.
ACCESSION   DASWNU010000054 DASWNU010000000
VERSION     DASWNU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235689"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VGB57_01120"
     CDS             <1..957
                     /locus_tag="VGB57_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HEY0129974.1"
                     /translation="VAVAIALLGPRSWPLRIGLAAAAGAALAAGFALAWPHCLGRLEG
                     ASPELVRLWLGNVREARPLYMHGYSTAISVAALPVAGLAGYAAMLWRHRRDEEKLVAW
                     GATAAPALLAALLLLWQSRAGPAAQLLAVPGATALVWLAIVSRFMLVRVAGTLALFLI
                     ASGLLPQQILRYFPPKPRPGMTVVNKANNLCPTLWALKPVAQVPRGQVLTFVDLGPRL
                     IALTHHDAVAGPYHRNGQDIIDVMRAFRGTPEAARATVERRGIDYVLICPGLSESTVY
                     SSQARQGFYMQLVRGKVPVWLQPVPLPANSPYRMWRVVTPRS"
     gene            complement(941..1564)
                     /locus_tag="VGB57_01125"
     CDS             complement(941..1564)
                     /locus_tag="VGB57_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY0129975.1"
                     /translation="MIELFLSAFVTFLVVIDPPGCAPIFAGLTREATAAQRRSMALRA
                     CAIAAGILLFFGLLGEDLLRTLGISLAAFRIAGGIMLFLIALEMVFERRTQRRESRAR
                     EVDADPEHDDVSVFPMAIPMIAGPGSIASIMLLMARSNGHAESLVVLGALGAVLLLTL
                     VALLAAGWLMRLLGHRTEAMLTRLLGVILAALAAQFVIDGISVSFEV"
     gene            complement(1561..>1847)
                     /locus_tag="VGB57_01130"
     CDS             complement(1561..>1847)
                     /locus_tag="VGB57_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEY0129976.1"
                     /translation="ADIVVAAVGRPEMVRADWLKPGATVIDVGINRVPADEEGKTRLV
                     GDVDFAGASEVAAAITPVPGGVGPMTIAVLLRNTLVAAHSRAGLPPPDGL"
BASE COUNT          276 a          646 c          652 g          273 t
ORIGIN      
        1 gtcgccgtcg cgattgcctt gctggggccg cgttcctggc cgctccggat cgggctcgcc
       61 gcggcggcgg gcgcggcgct tgcggcgggc ttcgccctcg cctggccgca ttgcctcggc
      121 cggctcgaag gcgcctcgcc cgaactggtg cgattgtggc tcggcaacgt ccgcgaggct
      181 cggccgctct acatgcacgg ctattcgacc gcgatctcgg tcgccgcgct tccggtggcg
      241 gggctggccg gctatgccgc catgctctgg cgccatcgcc gggatgagga gaagctcgtc
      301 gcctggggcg cgaccgcggc gccggccttg ctcgccgcgc tcctgctcct ctggcagagc
      361 cgggccgggc cggccgccca gctgctcgcc gtgccgggag cgaccgcgct cgtctggctg
      421 gccatcgtca gccgcttcat gctggtccgg gtggccggaa cccttgccct cttcctgatc
      481 gcctcgggcc tgcttccgca acagatattg cgctacttcc cgcccaagcc gaggcccggg
      541 atgaccgtcg tcaacaaggc caacaatttg tgcccgaccc tgtgggcgct gaagccggtg
      601 gcccaggtgc cgaggggcca ggtgctgacc ttcgtcgatc tcggcccgcg gctgatcgcc
      661 ctcacccatc acgatgccgt ggccgggccc tatcatcgca acggccagga catcatcgac
      721 gtcatgcgcg ccttccgggg cacgcccgag gcggctcggg cgacggtgga gcggcggggg
      781 atcgattatg tcctgatctg ccccggcctc tccgaatcga ccgtctattc gagccaggcc
      841 aggcagggct tctacatgca gctcgtgcgc gggaaggtgc cggtctggct ccagccggtg
      901 cccctcccgg ccaactcgcc ctacaggatg tggcgggtgg tcacacctcg aagctgaccg
      961 agatcccgtc gatcacgaac tgggcggcga gggcggcgag gatcacgccc agcaggcgag
     1021 tcagcatcgc ctcggtgcgg tggccgagaa gacgcatcag ccagccggcg gccagaagcg
     1081 cgaccagggt gaggagaagc accgcgccca gggcgccgag caccaccagc gattccgcat
     1141 gcccattgct tcgcgccatc aggagcatga tggacgcgat cgagcccggg ccggcgatca
     1201 tcgggatcgc catcgggaag accgagacgt cgtcatgctc gggatcggcg tcgacctcgc
     1261 gggcgcggct ttcgcggcgc tgggtgcggc gctcgaacac catctccagg gcgatcagga
     1321 acagcatgat cccgccggcg atgcggaagg cggcgaggct gatcccgaga gtcctcagca
     1381 gatcctcgcc gagcaggccg aagaagagga ggatcccggc ggcgatggcg caagcgcgca
     1441 gggccatcga ccggcgctgc gcggcggtgg cctcacgggt cagcccggcg aagatcggag
     1501 cgcagccggg agggtcgatc accacgagga aggtgacgaa agccgagagg aagagctcga
     1561 tcacaggccg tctggcggag gcaatcccgc cctcgaatgc gccgccacca gcgtgttgcg
     1621 aagaaggacg gcgatggtca tcgggccgac cccgcccgga acaggcgtga tcgccgcggc
     1681 gacttcggaa gcaccggcga aatcgacgtc gccgacgagt cgcgtcttgc cttcctcgtc
     1741 cgcggggacc cggttgattc cgacgtcgat gacggtcgct ccgggcttca gccagtccgc
     1801 cctcaccatt tccggccggc cgacggcggc gacgacgatg tcggcgc
//
LOCUS       DASWNU010000055         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_417516, whole genome shotgun sequence.
ACCESSION   DASWNU010000055 DASWNU010000000
VERSION     DASWNU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417516"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VGB57_01135"
     CDS             complement(<1..167)
                     /locus_tag="VGB57_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEY0129977.1"
                     /translation="MTHPVIHIWRLLKWGRVLARHGALRGIERDPNTPQPVRRLARIA
                     RFGARVPRIPA"
     gene            complement(268..996)
                     /locus_tag="VGB57_01140"
     CDS             complement(268..996)
                     /locus_tag="VGB57_01140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0129978.1"
                     /translation="MDKVSFGFEEVAPEEKTRRVGAVFSSVARRYDLMNDLMSAGLHR
                     QWKDLFVRRVRPRKGESILDMAGGTGDVAFRLARDGARVMVADINPDMLEVGRQRARK
                     RRIEGLAWAIENAETLSFGDAAFDGYTIAFGIRNVTDIGAALGEAHRVLKTGGRFYCL
                     EFSTTRWPGFASLYDAYSMHAVPRIGKIVASDEESYRYLVESIRRFPPMEKFSEMIGK
                     AGFAQVKAEPILGGLVAIHSGWKI"
     gene            1146..>1809
                     /gene="mutM"
                     /locus_tag="VGB57_01145"
     CDS             1146..>1809
                     /gene="mutM"
                     /locus_tag="VGB57_01145"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY0129979.1"
                     /translation="MPELPEVETTVAGLVPVLQGRRLVSVEARRADLRRAFPPDLRQR
                     LTGAVVTHLSRRAKYGLIATDRGDTLVFHLGMSGRWRIDPAELGAHDHFLLETDAGRR
                     LSLNDPRRFGSLDLVPTEALEATAPFDTMGPEPLGDEFDAHRLLEALAGRSAPIKAML
                     LDQRIVAGLGNIYVCEALHMAGIAPSRAAGRISRPRLERLVEAIRAVLLAAIEAGGSS
                     RSE"
BASE COUNT          272 a          650 c          599 g          288 t
ORIGIN      
        1 taggcgggga tccggggcac ccgagcgccg aagcgggcga tccgggccag ccgacgaacg
       61 ggctgcggcg tgttcgggtc gcgctcgatc ccgcgcagag cgccgtgccg cgccagcacc
      121 cggccccatt tcagaagccg ccagatatgg atgacgggat gggtcattgg gccgcgtcat
      181 cccggcgaaa gccgggacct cgggacgaga agtctcggcg ggtctccccg aggtccgggc
      241 tttcgccggg atgacgaagc gggcccatca gatcttccat ccgctgtgaa tggccaccag
      301 cccacccagg atcggctcgg ccttgacctg agcgaagccc gccttgccga tcatctccga
      361 gaacttctcc atcggcggga accggcggat cgactcgacg aggtagcggt agctctcctc
      421 gtcgctcgcc acgatcttcc ctatccgcgg caccgcgtgc atcgagtaag catcgtagag
      481 gctggcgaag ccgggccagc gggtcgtcga gaattcgagg cagtagaaac ggccgccggt
      541 cttcagcacc cggtgcgctt ccccgagcgc cgcgccgatg tcggtcacgt tccggattcc
      601 gaaggcgatc gtgtagccgt cgaacgcggc atccccgaag cttagcgtct cggcattctc
      661 aatcgcccag gcgaggcctt cgatacgccg ctttcgcgcc cgctgccggc cgacctcgag
      721 catgtccggg ttgatgtcgg ccaccatcac cctcgcgccg tcgcgcgcga gccggaaggc
      781 gacgtcgccc gtgcctccgg ccatgtcgag gatcgactcg cccttgcgcg gccttacccg
      841 gcggacgaac agatccttcc actgccggtg cagtccggcc gacatgagat cgttcatcag
      901 atcgtagcgc cgtgcgacgc tggagaagac cgcgccgacg cgccgggtct tctcctcggg
      961 cgccacctct tcgaagccga aagaaacctt gtccatcgcg cccgcctcta gcaggggttt
     1021 cagcgcatgt gcactcttcc cgtcatgcca gcgaaggctg gcatcccggc gggtgaaatg
     1081 cgcctgcgtt cttcacccgc tggggcccca gctttcgctg gggcgacggg tatgggaatg
     1141 cgccaatgcc tgagctgccc gaggtcgaaa ccacggtggc gggactggtt cccgtccttc
     1201 aggggcgccg gctcgtttcc gtcgaagcgc gccgcgccga cctccgccgc gccttcccgc
     1261 cggaccttcg ccagcggctg accggggccg tggtcacgca tctgtcgcga cgggccaaat
     1321 acggcctcat cgcgaccgac cggggcgaca cgctcgtctt ccacctgggc atgtcgggcc
     1381 gctggcggat cgacccggcg gagctcggcg cccacgacca tttcctgctc gagacggatg
     1441 ccggccggcg gctctcgctc aacgatccgc gccggttcgg atcgctggac ctggtgccta
     1501 ccgaagcgct cgaagccacc gcgcccttcg acacgatggg cccggagccc ctcggcgacg
     1561 agttcgacgc gcaccgcctg ctcgaagcgc tggcgggccg ttccgctccg atcaaggcca
     1621 tgctcctcga ccagaggatc gtcgccggcc tcggcaacat ctacgtctgc gaggccctgc
     1681 acatggccgg catcgccccg tcgcgggcgg ccggccggat ctcgaggccg cggctggagc
     1741 ggctggtcga ggcgataagg gccgtcctgc tcgcagccat cgaggcgggc ggctcgtcga
     1801 gatcggaag
//
LOCUS       DASWNU010000056         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_79760, whole genome shotgun sequence.
ACCESSION   DASWNU010000056 DASWNU010000000
VERSION     DASWNU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79760"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..243
                     /locus_tag="VGB57_01150"
     CDS             37..243
                     /locus_tag="VGB57_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129980.1"
                     /translation="MGAAMGERGGSAWEEDMAALRRGLQLAGVAVASAAATAGLVIAI
                     GTSVAPKSEPSAGVIQIAALAPAR"
     gene            323..1402
                     /gene="pseB"
                     /locus_tag="VGB57_01155"
     CDS             323..1402
                     /gene="pseB"
                     /locus_tag="VGB57_01155"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HEY0129981.1"
                     /translation="MYDSAARRFSEDTPFRDFAPKTRALDGKVVLITGGTGSFGRRFV
                     REILATQTPRKLIVLSRDELKQYEMKEELAREFPKETLDRMRFFLGDVRDRSRLELAF
                     RGVDVVVHAAALKQVPAAEYNPSECIQTNVMGAENVVWACLHNHVEKVVALSTDKACS
                     PINLYGATKLASDKTFVAANNMAGDIGTRFSVVRYGNVMGSRGSVAPLFRRLMDNGAV
                     DLPITDARMTRFWITLEQGVNFVLSSLEIMRGGEVFVPKIPATTVTALAEAMAPALPT
                     RVVGIRPGEKLHEMMISSDDARQTVDLGDRYAIEPDFVEYPRESFVKTGDGESVEEGF
                     AYTSDTGEERLDARGLMALLERAHA"
BASE COUNT          241 a          534 c          501 g          236 t
ORIGIN      
        1 gtgtattgct atggttaacc cgctgtggat aagcgggtgg gggcagccat gggcgaacgg
       61 ggcggatcgg cgtgggaaga ggacatggcg gccctgcgcc gcggtcttca gctcgccggc
      121 gtcgccgtag cctcggcggc cgcgacagcc ggcctggtga tcgccatcgg gacttccgtg
      181 gcccccaagt ccgagccctc cgccggcgtc atccagatcg ccgcactcgc gccagcgcgt
      241 taacggacat cgttaacgct cgttaaaggc ccgtcgcgcg aaagtccgat cctcacggat
      301 tcgtccgtcc ggatcgcttg ccatgtatga ctccgccgcc cgccgcttct ccgaagacac
      361 gccgttcagg gacttcgcgc cgaagacccg cgccctcgac ggcaaggtgg tgctgatcac
      421 cggcggcacc ggctccttcg ggcgtcgctt cgttcgggag atcctggcga cccagacgcc
      481 gcgcaagctg atcgtcctca gccgcgacga gctgaagcag tacgagatga aggaggagct
      541 ggcccgggag tttccgaagg agacgctgga ccggatgcgc ttcttcctcg gcgacgtgcg
      601 cgaccgctcg cggctggagc tggccttccg cggcgtcgac gtggtcgtcc acgccgccgc
      661 cctgaagcag gtgccggcgg ccgaatacaa cccgtccgaa tgcatccaga ccaatgtcat
      721 gggcgccgag aacgtggtct gggcctgcct gcacaatcac gtcgagaagg tcgtggccct
      781 gtcgaccgac aaggcctgct cgccgatcaa cctttatggc gcgaccaagc tggcgtcgga
      841 caagaccttc gtcgccgcca acaacatggc cggcgacatc ggcacgcgct tctcggtggt
      901 ccgctacggc aacgtcatgg gctcgcgcgg ctcggtcgcg ccgctgttcc gccgcctgat
      961 ggacaacggg gccgtcgacc tgccgatcac cgacgcgcgc atgacccgct tctggatcac
     1021 cctcgagcaa ggcgtgaact tcgtcctgtc gtcgctcgag atcatgcgcg ggggcgaggt
     1081 cttcgtgccc aagatcccgg cgaccaccgt caccgccctg gccgaggcca tggctccggc
     1141 cctgccgacg cgcgtcgtcg gcatccgccc gggcgagaag ctgcacgaga tgatgatctc
     1201 gtcggacgac gcccgccaga ccgtggacct cggcgaccgc tacgccatcg agcccgattt
     1261 cgtcgaatac cctcgcgaga gcttcgtgaa gaccggcgac ggcgagtcgg tcgaagaggg
     1321 cttcgcctac accagcgaca cgggcgagga gcgcctcgac gcccgcggcc tcatggccct
     1381 gctggaacgg gcgcatgcct aggggagacg gcccgatggc ggaggccagg cccttcctgc
     1441 cctacggccg ccagagcatc gacgacgccg acgtggccgc cgtggccctg gccctgcgcg
     1501 gcgacttcct ca
//
LOCUS       DASWNU010000057         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_495095, whole genome shotgun sequence.
ACCESSION   DASWNU010000057 DASWNU010000000
VERSION     DASWNU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495095"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VGB57_01160"
     CDS             <1..329
                     /locus_tag="VGB57_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0129982.1"
                     /translation="GEPILTLKDGARTHRVAETDILFIRAADDYCEAVLGDGRAMLVT
                     MTLSRLLETLPERFVRVHKSYAVNRAHVAGTAPRPGGGRMLRLSEGSEVPVGRSYAQR
                     VAAAFS"
     gene            complement(322..939)
                     /locus_tag="VGB57_01165"
     CDS             complement(322..939)
                     /locus_tag="VGB57_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0129983.1"
                     /translation="MSVAHWVRTLGLRPGRASWYSGAMFTDMRDGTAEEWRHIAGEHA
                     RHQAAAAPRQIMESLKRLEAIEVGFAANQLTHSLMAGTLARRSGASDEEVVAALCHDL
                     GKTFSIPNHGPIAAEMLKPYVSDDIYQAVYWHQEFQGRYYFEHLGKDPEGRARFAGES
                     WYGFAEKLVDEWDAPAFDPGFPVDSLESFEPEVTRVFSRPRAMVS"
     gene            complement(1035..>1907)
                     /locus_tag="VGB57_01170"
     CDS             complement(1035..>1907)
                     /locus_tag="VGB57_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129984.1"
                     /translation="ALHHGRVVSPELYRAMITPVTLADGSTHPYGFGLAMEKVRGRDA
                     IQHNGGISGFNTDSIYIPGEDVFVAIFTNSDEPATHPTLISRRLAALAVGDPFRTFTA
                     VELAPDAVAPFFGVYAIGDTGVSRRFYSRDGKFYTLRDGGGESEVRPAGDGHFFYPGN
                     NNWFRLLRRPDGALVMEMHQDGANEGELATRTGDIPPERPAASVSREILQSYVGRYET
                     VGPAAEIALREDGVLTLQLAGQPAFPLRPTSDTEFTIEQVGARIVFHGENGQVNRFVI
                     HQGGQQLEGRRAAR"
BASE COUNT          288 a          673 c          643 g          303 t
ORIGIN      
        1 ccggcgagcc gatcctcacc ctcaaggacg gcgcgcgcac gcaccgagtc gccgagaccg
       61 acatcctctt catccgcgcc gccgacgatt attgcgaggc ggtcctcggc gacgggcgcg
      121 cgatgctggt gacgatgacg ctatcgcgcc tgctcgagac cctgcccgag cgcttcgtgc
      181 gggtgcacaa aagctatgcc gtgaaccgcg cccatgtcgc cggcaccgcc cccaggccgg
      241 gcggcggccg catgctcagg ctcagcgagg gcagcgaggt cccggtcggg cgaagctacg
      301 cacagcgcgt cgcggcggcg ttcagctgac catcgcccgc ggtcggctga acacccgagt
      361 cacttccggc tcgaagcttt cgaggctgtc gaccgggaaa cccggatcga aggccggcgc
      421 gtcccactcg tcgaccagct tctcggcaaa gccataccag ctctcgcccg cgaaccgcgc
      481 ccgcccctcc gggtccttgc cgagatgctc gaaataatag cggccctgga attcctggtg
      541 ccagtagacc gcctgataaa tgtcgtccga cacatagggc ttcagcatct cggcggcgat
      601 cgggccgtga ttggggatcg agaatgtttt gcccaaatcg tggcacaggg ccgccaccac
      661 ttcctcgtcg ctcgcgcccg aacggcgcgc cagcgtcccg gccatcaggc tgtgggtcag
      721 ctggttggcc gcgaagccga cctcgatcgc ctcgagccgc ttcaggcttt ccatgatctg
      781 ccgcggcgcc gccgccgcct ggtgccgggc gtgctccccg gcgatgtgcc gccactcctc
      841 ggcggtgccg tcgcgcatgt cagtgaacat agcgccgcta taccagctcg cgcggcccgg
      901 gcgaagcccc agggtccgga cccaatgggc tacgctcaac gaaagcccct ccccctcgtg
      961 gggaggggtt ggggtggggg cgcgagcctg tccaggctcg caaaagactc tcttcggtcg
     1021 cgcccgtccc gcgatcagcg ggcggcgcgg cgtccttcca gctgttgccc gccctggtgg
     1081 atgacgaagc ggttcacctg gccgttctcg ccgtggaaga cgatccgggc cccgacctgc
     1141 tcgatcgtga actcggtgtc gctggttggg cggagcggaa aggcgggctg accggcgagc
     1201 tggagcgtca gcactccatc ctcgcgcagc gcgatctcgg ccgccgggcc gaccgtctcg
     1261 tagcggccga cataggattg caggatctcg cggctgaccg acgccgccgg ccgctccggc
     1321 gggatgtcgc cggtccgcgt cgccagctcg ccttcgttgg cgccgtcctg gtgcatctcc
     1381 atcaccagcg cgccgtcggg gcggcgcagg agccggaacc agttgttgtt ccccggatag
     1441 aagaagtggc cgtcgcccgc cggccgcact tcgctctccc cgcccccgtc gcgcaatgtg
     1501 tagaatttgc cgtcgcgcga atagaagcgc cgcgacacgc ccgtgtcgcc gatcgcatag
     1561 actccgaaga agggcgcaac cgcgtccggg gcgagctcga ccgccgtgaa ggtccggaag
     1621 ggatcgccga ccgcgagcgc ggcgagccgc cgcgagatga gggtcgggtg agtcgcgggc
     1681 tcgtcgctgt tggtgaagat tgcgacgaac acatcctcgc ccggaatgta gatgctgtcg
     1741 gtgttgaagc ccgaaatgcc gccattatgc tggatggcgt cgcggccgcg caccttctcc
     1801 atggcgaggc cgaagccgta aggatgggtg ctcccgtcgg cgagcgtcac cggcgtgatc
     1861 atcgcccggt agagttccgg gctcaccacc cgcccgtggt gcagggc
//
LOCUS       DASWNU010000058         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_27551, whole genome shotgun sequence.
ACCESSION   DASWNU010000058 DASWNU010000000
VERSION     DASWNU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27551"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1807)
                     /locus_tag="VGB57_01175"
     CDS             complement(<1..>1807)
                     /locus_tag="VGB57_01175"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEY0129985.1"
                     /translation="DGTPVKRARYSALTKDEIERAFNNLDELSMPLANAGAARQDIDL
                     LWGATLTRAVSLASRRFGSNFLSVGRVQSPTLGLIVEREMERRAHVAKPFWEVFAKFD
                     HPDGSFETHHKVDKFWEQAEADAAIAGTSNPGTVKAVTARKNTRRPPTPYNTTAFSTD
                     ASSRLGITPASAMRIAEDLYMDGFISYPRTDNTVYPSSLPTRELVSSLVRIKEFSAAS
                     GLLDGELKPTRGKKETTDHPPIYPTQAVHPGALEGPRKRVYELVVRRFLATFSPPMIT
                     ESTRADIEAGDQTYFVRGSVVVDPGYAAIYTYARSADEEIPKLEEGQTLQLDGEPWIV
                     DKETQPPGRISQAKLIEMMEERGLGTKATRADIIQKLFDRGYVYSNPPIPTETGIALY
                     EAFKNYVPAMATPEMTAQLEAEMDQIAAGEMSKGSVVGESRELLHKTWTEIDGSREDL
                     AKVIWKGMDEDRILGPCKVCEEAGRTKEDGSPNMLRIIRAKKSGKRFVGCSGWSADGG
                     DGSCDQTFPLPQRGDVFRLEERCSICDRTPRLKVQPFRGRPWNLCLHDDCESMQEMKK
                     RRAEREAAKAAKAAMEKKAPPKGDEDAAPKKRKVKT"
BASE COUNT          221 a          552 c          668 g          366 t
ORIGIN      
        1 gttttgactt ttctcttctt cggagcggcg tcctcgtcgc ctttcggcgg cgccttcttc
       61 tccatcgccg ccttcgcggc tttcgcggct tcgcgctcgg cccggcgttt cttcatctcc
      121 tgcatcgact cacagtcgtc gtggaggcag aggttccagg ggcggccgcg gaacggctgg
      181 actttgaggc gcggggtgcg atcgcagatc gagcaccgct cctcgagccg gaagacgtcg
      241 ccgcgctggg gcagcgggaa ggtctggtcg caggagccgt cgccgccgtc ggcgctccag
      301 ccgctgcagc cgacgaagcg tttgcccgat tttttcgcgc ggatgatccg cagcatgttc
      361 ggcgagccgt cctccttggt gcgaccggct tcctcgcaga ccttgcaggg gccgaggatg
      421 cggtcttcgt ccatcccttt ccagatcact ttcgcgaggt cctcgcggct gccgtcgatt
      481 tcggtccagg ttttgtgcag gagctcgcgg ctctcgccga cgacggaccc cttggacatc
      541 tcgccggcgg cgatctggtc catctccgcc tccagctggg cggtcatctc cggcgtcgcc
      601 atcgcgggca cgtagttttt gaacgcctcg tagagggcga tcccggtctc ggtcgggatc
      661 ggcgggttgc tgtagacgta gccgcggtcg aagagcttct ggatgatgtc ggcgcgggtc
      721 gctttggtgc cgaggccgcg ctcctccatc atctcgatca gtttcgcctg ggagatgcgc
      781 ccgggcggct gggtctcctt gtcgacgatc cagggctcgc cgtcgagctg cagggtctgc
      841 ccctcttcca gcttcgggat ctcctcgtcg gccgaacggg cgtaggtgta gatggcggca
      901 tagccggggt cgacgacgac cgagccgcgg acgaagtagg tctggtcgcc ggcttcgatg
      961 tcggcccggg tcgactcggt gatcatcggc gggctgaagg tggcgaggaa gcgacggacg
     1021 acgagttcgt agacccgttt ccgcgggccc tcgagggcgc cggggtggac cgcctgggtc
     1081 gggtagatcg gcgggtggtc ggtcgtctct tttttgccgc gggtcggttt cagctcgccg
     1141 tcgagcaggc ccgaggcggc ggagaactct ttgatccgga ccagcgagga gaccagctcc
     1201 cgggtcggca gcgaggacgg atagaccgtg ttgtcggtgc gcgggtagga gatgaacccg
     1261 tccatgtaga ggtcctcggc gatccgcatc gcgctggcgg gggtgatccc gaggcgactg
     1321 gaggcgtcgg tgctgaaggc ggtggtgttg tagggcgtcg gcggccggcg ggtgttcttc
     1381 cgcgccgtca ccgctttcac ggtgcccggg ttcgaggtgc cggcgatcgc cgcgtcggct
     1441 tcggcctgct cccagaattt gtccactttg tggtgggtct cgaagctgcc gtcgggatgg
     1501 tcgaacttgg cgaacacttc ccagaacggt ttcgcgacgt gggcgcgccg ttccatctcg
     1561 cgctcgacga tcaggcccag ggtcgggctc tggacgcggc cgaccgagag gaagttggag
     1621 ccgaagcggc ggctggccag ggagacggcg cgggtcaggg tcgcgcccca gagcaggtcg
     1681 atgtcctggc gggcggcgcc ggcgttggcg agtggcatcg agagctcgtc gaggttgttg
     1741 aacgcgcgct cgatctcgtc cttggtgagt gcggagtagc gcgcgcgctt gacgggggtg
     1801 ccgtcgt
//
LOCUS       DASWNU010000059         6503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_79935, whole genome shotgun sequence.
ACCESSION   DASWNU010000059 DASWNU010000000
VERSION     DASWNU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79935"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VGB57_01180"
     CDS             complement(<1..458)
                     /locus_tag="VGB57_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY0129986.1"
                     /translation="MRAVSWFKDLISSERGNVFVVAAAVMPLLLGSAAFAVDTIQLSV
                     WKRQLQRAADSGAIAGAYALSLGDEEHDAVHRDLDKNKFPTLTQEEQITLGSRPGFTQ
                     TVRVSLTATRTLPFLSIFTKRPTTMVANATAGLENSGEFCMVSLYDGPDAG"
     gene            complement(793..1377)
                     /gene="msrA"
                     /locus_tag="VGB57_01185"
     CDS             complement(793..1377)
                     /gene="msrA"
                     /locus_tag="VGB57_01185"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0129987.1"
                     /translation="MRLILVALLLLAGAGAAPAQDRPKLASAVFAGGCFWCTESDFDK
                     IPGVVSTTSGYSGGRSANPTYEQVSAGGTGHIEVVRIVYDPARVSYQKLLARFFRTID
                     PLDGGGQFCDRGSQYRSAIFVASPAQRRLAEATKARAAALLKKPVATQILPAAPFYAA
                     ETYHQDYYRKNPTKYKYYRWRCGREARLAKVWGS"
     gene            complement(1386..1895)
                     /gene="msrB"
                     /locus_tag="VGB57_01190"
     CDS             complement(1386..1895)
                     /gene="msrB"
                     /locus_tag="VGB57_01190"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0129988.1"
                     /translation="MSDSRVSRRGFIGSAAAVAVAAGTGFALFGRDGAPAEAAERFEV
                     TKTPAEWRRLLGAQRYAVLREAATERAGSSPLDREKRRGTFLCAGCDLPVYGSAAKFD
                     SGTGWPSFTAPLPNAVRTRPDRVLFTVRTEVHCRRCGGHLGHVFDDGPRPTGKRFCMN
                     GLALKFRPA"
     gene            complement(1976..3058)
                     /locus_tag="VGB57_01195"
     CDS             complement(1976..3058)
                     /locus_tag="VGB57_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0129989.1"
                     /translation="MRRTAALIVGGGPAGSAAAIALARAGIGAELIERTEGPHDIVCG
                     GFLGWDALDALDRLGLDPAVLGARPIRRLRLISAKRSVEMPLPRLAAGLSRRTLDEAM
                     LAAAHRAGAAVRRGRTVRAADLAERRVRLDDDEGLAGEALILATGKHELRGAARPIDI
                     STRPLGLRSSLEAGPALAQALEGVIELHLYDGGYAGLLMQEDGRSNFCLSASRDRLKE
                     AGGIEPLVARLADELPAFGERLRQGRPGPWSAVSGVPYGWRASETVEGVYRVGDQSAV
                     IASLAGDGIAIALESGVAAAGAIAAGKPAADFQRDWARRARRPVGLAEVLRRSAENPM
                     ARQAMMGLLGWFPSLAPLAARLTRIG"
     gene            complement(3037..3726)
                     /locus_tag="VGB57_01200"
     CDS             complement(3037..3726)
                     /locus_tag="VGB57_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0129990.1"
                     /translation="MRSLKQRVRDEEQMDDPSLDPEVYDEVLKGLGRVNAWTFAARPT
                     LSFLARATRGRDGFSLLDVGSGEGGMLRTIAKWARRQGLAARLTGVDLNPKSEGVARA
                     KTPADMGIAYRTGDYRDLGRFDFVISSLVAHHMTDSQLLDFLRWMEANSDRGWFVNDL
                     HRHGFAWFGFPLLATLLRVHRIVREDGRLSIARSFRPVDWRSILAEAKVAEGVKVRRY
                     FPFRLCVERLR"
     gene            complement(3723..4823)
                     /locus_tag="VGB57_01205"
     CDS             complement(3723..4823)
                     /locus_tag="VGB57_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEY0129991.1"
                     /translation="MQFSTLARESRTSQAHLNAIGTAVPAHDVHKAFIGWAEARLREP
                     RARALFTRMAARSGIEHRRSVLPPGPYGASPVDPGGFYHGALPTTGARMEIYAQAAPR
                     LALEAIGKLDRAMAPGDISHLIVASCTGFVAPGIDQIIARELGLGGHVERTLVGFMGC
                     YAAVSSLRLAHHIVRSEPAARVLVVTVELSSLHLQYEEDVERLLAALQFGDGATASLV
                     TAEPSGFALSRPFAATLPDSEQLIRWRVGDHGFVMDLSGEVPGRIGEALRDRGVREAM
                     LGNEAPEAFDSWAVHAGGRSVLDAVEHGLDLPPEALAQSREILSRYGNMSSSTLPFVL
                     EGMLADPARRRGIALAFGPGLAAEGFHFERAA"
     gene            complement(5006..5081)
                     /locus_tag="VGB57_01210"
     tRNA            complement(5006..5081)
                     /locus_tag="VGB57_01210"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5046..5048),aa:Phe,seq:gaa)
     gene            complement(5120..5314)
                     /gene="yacG"
                     /locus_tag="VGB57_01215"
     CDS             complement(5120..5314)
                     /gene="yacG"
                     /locus_tag="VGB57_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY0129992.1"
                     /translation="MSTPNLPKRGKGCPLCGKPSGPEHKPFCSRGCRDRDLLNWLGDS
                     YRVPGPPAVENAEGGLDSEG"
     gene            complement(5292..6263)
                     /locus_tag="VGB57_01220"
     CDS             complement(5292..6263)
                     /locus_tag="VGB57_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEY0129993.1"
                     /translation="MAEWLYEEGIGENRAILVEDEDILVAAIELPGEMRCGSVLAGRL
                     VRIAIPGRRGIVSTDRGEVMVEPLPAILTEGQKVRVEIVREPIAEPGHAKFAKGRISD
                     AEEREGPTLAQRIGSPRPLRPLGPDLFHEAGWSELIDEALGEPIDFPGGELRMSLTPA
                     MTLFDVDGFLPPVELATAGAEAAALAIRRLDIGGSIGIDLPTIASRDERLRVVAALDA
                     VLPQPFERTAVNGFGFLQVVRRRERRSLPELLQWDPVGAAARDLLRTAERTVGGCELA
                     AAPAVIARLRSSPDWLETLARRIGGPVALREDSSFTTWQSRVHAKPS"
     gene            complement(6256..>6503)
                     /locus_tag="VGB57_01225"
     CDS             complement(6256..>6503)
                     /locus_tag="VGB57_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0129994.1"
                     /translation="DAEGRTRIRLSTSVVAFKRLSRVEIDAYLASGEWRGKAGGYAIQ
                     GRAEALVRMLSGSHSGVMGLPLYETRALLRAAGFPLG"
BASE COUNT         1060 a         2200 c         2298 g          945 t
ORIGIN      
        1 ccggcgtcgg gtccgtcgta gagcgagacc atgcagaact cgccgctgtt ttcgaggccg
       61 gcggtggcgt tggcgaccat ggtcgtcggg cgcttggtga agatcgacag gaacggcagc
      121 gtgcgggtcg ccgtcaggct cacccgcacc gtctgggtga agccggggcg ggagccgaga
      181 gtgatctgct cttcctgggt gagggtcggg aatttgttct tgtcgaggtc gcggtggacg
      241 gcgtcatgct cctcgtcgcc cagcgacagc gcataagcgc cggcgatcgc gcccgaatcg
      301 gcggcgcgct ggagctggcg cttccagacg ctgagctgga tggtgtcgac cgcgaaggcg
      361 gcggagccca gaagcagcgg catcaccgcc gcggcgacca cgaacacgtt gccgcgttcg
      421 ctcgaaatca gatccttgaa ccagctcact gccctcatcg tccctgttcc tcgtcggtgc
      481 aaagcgcagc tagaggggac cgcgttaggg ccggccggtt agggactcat tgagcctatt
      541 ggttaccgcc ggattcagct tgttttgtct gcagagcgat cgcgggagat gcgtgacctt
      601 cgcgtatctt cgcgtgaatc gaaaaaagta aatgggctca cgcgaggggg cgcgaagaac
      661 gcgaagaaga ggcgacgctt tgtccctcgg cacccgttct cgtaccctgg tgtccttctg
      721 cgaagaacgg gcctgcggcc gtcagtaaag acactaggac gcccgcagga agacgtaggc
      781 tgtggcccgg gtctagctgc cccacacctt cgccaggcgg gcttctcggc cgcagcgcca
      841 gcggtagtat ttatatttcg tggggttctt tcggtagtaa tcctggtgat aggtttcggc
      901 cgcgtagaag ggggcggcgg ggaggatctg ggtggcgacc ggcttcttga gcagggccgc
      961 ggcccgcgcc ttggtggctt cggcgaggcg gcgctgggcg ggactggcga cgaagatggc
     1021 ggagcgatac tggctgcccc ggtcgcagaa ctggccgccg ccgtcgagcg ggtcgatggt
     1081 gcgaaagaag cgggcgagaa gcttctggta gctcacccga gcgggatcgt agacgatcct
     1141 caccacttcg atatggccgg tgccgccggc cgagacctgc tcgtaagtgg ggttggccga
     1201 cctgccgccg gaatagccgg aagtggtcga gaccaccccg gggatcttgt cgaaatcgga
     1261 ttcggtgcac cagaagcagc cgccggcgaa gaccgcggag gcgagcttcg gcctgtcctg
     1321 ggccggcgcg gcgccggcgc ccgcgagcag gagcagtgcg acgaggatga gtctcatcgc
     1381 ggccatcatg ccggccggaa cttgagggcg agcccgttca tgcagaagcg ctttccggtc
     1441 ggccggggcc cgtcgtcgaa gacgtggccg agatggccgc cgcagcgccg gcaatggacc
     1501 tcggtccgaa ccgtgaacag gacccggtcg gggcgggtgc gcaccgcatt gggaagcgga
     1561 gcggtgaagc tcggccagcc ggtgccgctg tcgaacttgg cggccgagcc gtagaccgga
     1621 aggtcgcagc ccgcgcacag gaaggtgcct cgccttttct ccctgtcgag cgggctcgag
     1681 ccggcgcgct cggtggcggc ctcgcgaagc actgcataac gctgcgctcc gagcagccga
     1741 cgccattcgg cgggagtctt cgtcacctcg aagcgctccg ccgcctcggc gggggcgccg
     1801 tcgcggccga aaagggcaaa gcccgtgccc gcggcgaccg cgaccgccgc cgccgagccg
     1861 atgaagccgc gcctggaaac tctgctgtcg ctcataaggg ccgccttttg tggaggcgac
     1921 caatgtagga aggccgagtg ggttcgtcac cctgggcaaa aggcgggggg gataatcaac
     1981 ctatccgagt cagcctcgcc gccagcggcg cgagcgaggg gaaccagccg agcaggccca
     2041 tcatcgcctg ccgggccatc ggattttcgg ccgagcgccg aagcacctcg gccagcccga
     2101 ccggccgccg cgcccgccgc gcccagtcgc gctggaagtc cgccgccggc ttgccggccg
     2161 cgatcgcccc ggccgccgcc actccgctct ccagcgcgat ggcgataccg tcgccggcca
     2221 gcgaggcgat gaccgcggac tgatcgccga cccggtacac gccttccacg gtctcactcg
     2281 cccgccagcc gtagggaacg cccgagaccg cgctccacgg gccgggccgg ccttgccgca
     2341 gccgctcgcc gaaggcggga agctcgtcgg ccagccgagc caccaacggc tcgattccgc
     2401 cggcctcctt cagccggtcg cgtgatgcgg acaggcagaa attggagcgg ccgtcctcct
     2461 gcatcagcag cccggcatag ccgccgtcgt aaaggtgaag ctcgatcacg ccctccagcg
     2521 cttgggcgag cgcggggccg gcctcgagcg agcttcgaag gccgagcggg cgggtcgata
     2581 tgtctatcgg gcgggcggcg ccgcgaagct cgtgcttgcc cgtggcgagg atcagggctt
     2641 cgcccgccag cccttcgtcg tcgtcgagcc gcacccggcg ctcggccaga tcggccgctc
     2701 gaaccgtgcg tccccgccgg acagcggctc cggcccgatg cgccgcggcc agcatcgcct
     2761 cgtccagcgt ccgtcgggac aggccggcgg cgagccgggg caaaggcatt tcgacggacc
     2821 gtttcgccga gatcagccga agccggcgga tcggacgagc tccgagcacc gccggatcga
     2881 gcccgagccg atcgagcgcg tcgagcgcgt cccagccgag gaagccgccg cagacgatgt
     2941 cgtgcgggcc ttcggtgcgc tcgatcagct ccgccccgat cccggcgcgg gccaaagcga
     3001 tggccgcggc cgagccggcg ggcccgccgc cgacgatcag cgcagccgtt cgacgcacag
     3061 ccggaacggg aaatagcggc gcaccttcac gccctcggca accttggcct cggcgaggat
     3121 cgaccgccag tcgacgggac ggaaggaacg ggcgatcgaa agccggccgt cctcacggac
     3181 gatgcggtgg acccggagca gggtggccag caatgggaag ccgaaccagg cgaagccgtg
     3241 ccggtggaga tcgttgacga accagccccg gtccgaattg gcctccatcc agcgcaggaa
     3301 gtcgagcagc tgggaatcgg tcatatgatg ggcgacgagg ctgctgatca cgaagtcgaa
     3361 ccggccgagg tcgcgataat ccccggtccg gtacgcgatt cccatgtcgg ccggcgtctt
     3421 cgcccgggcc acgccttcgc tcttcgggtt taggtccacc ccggtgagcc gcgccgccag
     3481 gccttgccgc cgcgcccatt tcgcgatcgt ccggagcatt ccgccttcgc ccgagccgac
     3541 gtcgagcagg ctgaacccgt cgcggccgcg cgtggcccgg gcgaggaagg acaaggtcgg
     3601 ccgggccgcg aaggtccagg cattgacccg gccaagccct ttcagaacct cgtcatagac
     3661 ttcggggtcg agcgacggat cgtccatctg ctcttcgtcc cggacccgct gcttcagcga
     3721 cctcacgccg cccgctcgaa atggaagccc tcggccgcga ggccgggtcc gaaagcgagc
     3781 gcgattccgc gccgccgggc cggatcggcg agcatccctt cgagcacgaa tggcaaggtg
     3841 gaggaggaca tgttgccgta gcgggagagg atctcgcgcg attgcgccag ggcttccggc
     3901 ggcaagtcca ggccatgctc gaccgcatcg agcaccgaac ggccgccggc atggaccgcc
     3961 cacgaatcga aggcctccgg cgcctcgttg ccgagcatcg cctcgcgaac gccccgatcg
     4021 cgcagcgctt cgccgatccg gccgggcacc tcgcccgaca ggtccatgac gaagccgtga
     4081 tcgccgaccc gccagcggat cagctgctct gaatccggaa gcgtcgcggc gaacggccgg
     4141 gagagggcga agccgctcgg ctccgccgtg accagcgagg ccgtggcgcc gtcgccgaac
     4201 tgcagggcgg cgagcagccg ctcgacatcc tcctcatatt ggaggtggag gctggagagc
     4261 tcgaccgtaa ccaccagcac ccgggccgcg ggctcggagc ggacgatatg atgggcgagc
     4321 cggagcgagg agacggcggc gtagcagccc atgaatccga ccagggtgcg ctcgacatgg
     4381 ccgccgaggc cgagctcgcg ggcgatgatc tggtcgatgc cgggagcgac gaagccggtg
     4441 cagctggcga cgatcagatg gctgatgtcg cccggcgcca tcgcccggtc gagcttgccg
     4501 atcgcctcca gcgccagccg gggcgcggcc tgggcgtaaa tctccatccg cgcgccggtg
     4561 gtcggaagcg ctccgtggta gaagcccccg gggtcgaccg gcgaggctcc gtaaggcccc
     4621 gggggaagga ccgaccggcg atgctcgatc ccggagcggg cggccatcct cgtgaacagc
     4681 gcccgcgccc gcggctcgcg cagccgcgct tccgcccagc cgatgaaggc cttgtggacg
     4741 tcatgcgctg gaacggcggt gccgatcgcg ttgagatggg cctggctcgt acggctttcc
     4801 ctggcaagag tgctgaattg caacgaagat tagatgaatg aaaggggcgg aggttccgtt
     4861 agtcgcggcc ctgagcgggg gcaattacgc cttaaggcgg tactccgtca ttccggcgaa
     4921 ggccggaatc catgaacacg gagtgctggg cttcgtcccg cggtgttcat ggatcccgga
     4981 tcaggtccgg gatgacgacg ggagatggtg cccagggacg gaattgaacc gccgacaccg
     5041 tgattttcag tcacgtgctc taccaactga gctacctggg cgttccggcg gagccgaaga
     5101 cgagcgccta tagaggcgct cagccttcgc tgtccagccc gccttcggcg ttttccacgg
     5161 ccggcgggcc ggggacccgg taggaatcgc cgagccagtt cagcaggtcg cggtcccggc
     5221 agccgcggct gcagaagggc ttgtgctcgg ggccggaagg cttgccgcac agggggcagc
     5281 ccttgccgcg cttaggaagg tttggcgtgg acacggcttt gccatgtggt gaaggacgag
     5341 tcctcccgca aggcgaccgg gccgccgatt cgcctggcca gcgtttccag ccagtcgggg
     5401 ctcgaccgaa gccgggcgat cacggccggc gcggcggcga gctcgcagcc cccgacggtc
     5461 cgctccgcgg tccgaagcag atcgcgggcg gcggctccga ccggatccca ttgcagaagc
     5521 tcgggcaacg agcgccgctc gcggcgcctg accacctgga gaaagccgaa gccgtttacc
     5581 gccgtccgtt cgaaaggctg cggaagcacg gcgtcgagcg ccgcgacgac tcgcagccgc
     5641 tcgtcgcgcg aggcgattgt cggaagatcg atcccgatcg agccgccgat gtcgaggcgg
     5701 cggatcgcca gcgcggccgc ctccgcgccc gccgtcgcca gctccaccgg cggaagaaag
     5761 ccgtcgacgt cgaacagggt catcgccgga gttaggctca ttcgaagctc tccgccgggg
     5821 aaatcgatcg gctcccccag agcctcgtcg atcagttcgg accagccggc ttcatggaag
     5881 aggtctggac cgagcgggcg aagcgggcgg ggcgagccga tgcgctgcgc cagggtcggc
     5941 ccttcccttt cctccgcatc gctgatccgt cccttggcga atttggcatg gccgggctcg
     6001 gcaatgggtt cgcggacgat ctccacccgc accttctgcc cctcggtgag tattgccgga
     6061 agcggttcca ccatcacctc gccccggtcg gtggagacga tgccccggcg gcccggaatc
     6121 gcgatccgga cgagccggcc ggcgagcacc gagccgcagc gcatctcccc cggaagctcg
     6181 atcgcggcga ccaatatgtc ctcgtcctcg acgaggatcg cgcggttctc gccaatcccc
     6241 tcctcgtaga gccactcagc caaggggaaa gccggcggcg cgaagcagtg cccgggtctc
     6301 gtagaggggc aggcccatca cgcccgaatg gctgcccgac agcatccgca ccagcgcctc
     6361 cgctcggccc tgtatggcat agcccccggc cttgccccgc cactccccgc tggcgagata
     6421 ggcgtcgatc tccacacgag agaggcgctt gaaggcgacg acgctggtcg aaaggcgaat
     6481 tcgggtgcgg ccctcggcgt ccg
//
LOCUS       DASWNU010000060         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_53904, whole genome shotgun sequence.
ACCESSION   DASWNU010000060 DASWNU010000000
VERSION     DASWNU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53904"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1900)
                     /locus_tag="VGB57_01230"
     CDS             complement(116..1900)
                     /locus_tag="VGB57_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0129995.1"
                     /translation="MTATLPQLAAFGLLAGMMLLFIWGRIRYDMVAVLALLAALLLGL
                     VKPEDAFSGFADDIVIIVASALVISAAVERSGVVEAAIGRLSGGMSRMGSQLALLVGS
                     VTLLSALVKNIGALAMLMPAAFRMAKKSDSSPSCFLMPMAFGSLLGGLMTLVGTSPNI
                     IVSRVREEMTGEPFSMFDYTPVGAGIAIAGLIFLRFGYRLLPSDRRATPTMGEALDIE
                     DYATEAEVPPGSAAEDKSVAELRELLEKGVELTGIHRWDRRATLPLPDAVIRAGDVLI
                     LKGEPEALERAIATLSLELEGHHRDAAAGTASSNVGVIEAVIGRDSILAGTAAGRVAL
                     HDRHGVNLIAVSRRGERITRRLRDVVLCPGDIVVLQGALDTLPERLRELGCLPLAERS
                     LRLGNARRGLIPLAILAGAMALTAIGWVPVAVAFFGAAALIMLTGALPAREAYGRIEW
                     PILVMIAALIPVSDSLRTTGATELIAHYLSGLAATLPAWGAVALIMVAAMAVTPFLNN
                     AATVLVMAPIAASFAGGLGYRPEAFLMAVAVGAGCDFLTPIGHQCNTLVMGPGGYRFG
                     DYARLGAPLSVLVVVVGVPLILAVWPLR"
BASE COUNT          355 a          681 c          703 g          266 t
ORIGIN      
        1 atcgcggcca tcccgctact cctactgttc ggggctgcaa cgcccgagcc gcgttccagt
       61 gccgtatctc gttgacgtcg cttggaaatt gtaatgtcgg cgaggaattt tccacctacc
      121 gcagcggcca caccgcgagg atcagcggca ccccgaccac caccaccagc accgacagcg
      181 gcgccccgag cctcgcatag tcgccgaagc ggtagccgcc cgggcccatg acgagggtgt
      241 tgcactggtg gccgatgggc gtgaggaagt cgcagcccgc ccccaccgcc accgccatca
      301 aaaaggcttc gggacgatag ccgagcccgc ccgcgaagct cgccgcgatc ggcgccatga
      361 ccagcaccgt cgcggcgttg ttgaggaacg gggtgacggc catggccgcg accatgatca
      421 gggcgaccgc gccccaggcc ggcaaagtcg cggcaagccc cgacagataa tgggcgatga
      481 gctcggtggc gccggtggtt cgaagcgagt ccgacaccgg gatgagggcg gcgatcatca
      541 ccaggatcgg ccattcgatc cgtccgtaag cctcgcgcgc cggcagagcg ccggtgagca
      601 tgatgagcgc cgccgctccg aagaaggcca cggccaccgg aacccagccg atcgcggtca
      661 gggccatggc gccagcgagg atggcgagcg gaatgaggcc gcgcctggcg ttgccgagcc
      721 tcaggctgcg ctccgcgagc ggcaggcagc ccagctcgcg aagccgctcc ggcaaggtgt
      781 cgagcgcgcc ttgcaggacg acgatgtccc cggggcacag gacgacgtcg cgaagccggc
      841 gggtgatccg ctcgccgcga cgcgacacgg cgatgagatt gactccgtgg cggtcgtgga
      901 gggccacccg ccccgcggcg gttccggcca ggatcgagtc gcggccgatc accgcctcga
      961 tcaccccgac gttggacgag gcggtgccgg cggcggcatc gcgatgatgc ccttcgagct
     1021 ccaggctcag cgtggcgatc gcccgctcga gcgcctccgg ctcgcccttg aggatgagca
     1081 cgtcgccggc ccggatcacg gcgtccggca gcggcagggt cgcccgcctg tcccagcggt
     1141 ggattccggt cagctcgact cccttttcga gcagctcgcg aagctcggcc acgctcttgt
     1201 cttcggccgc cgagccgggc ggcacttcgg cctcggtcgc ataatcctct atgtcgagcg
     1261 cctcgcccat cgtcggagtc gccctgcggt cggaggggag aaggcggtag ccgaagcgaa
     1321 ggaagatgag gccggcgatc gcgatccccg cgccgaccgg cgtatagtcg aacatcgaga
     1381 agggctcgcc ggtcatctcc tcgcggaccc ggctgacgat gatgttgggc gaggttccga
     1441 cgagcgtcat cagcccgccg agcagcgagc cgaaggccat cggcatcagg aagcaggagg
     1501 gcgagctgtc cgatttcttg gccatccgga aggcggccgg catcagcatg gcgagcgccc
     1561 cgatattctt gaccagcgcc gacagcaagg tgaccgatcc gaccaggagg gcgagctgcg
     1621 agcccatccg gctcatgccg ccgctcagcc ggccgatcgc cgcctccacc accccggagc
     1681 gctctaccgc cgccgagatg accagggccg aagcgacgat gatgacgatg tcgtcggcaa
     1741 agccgctgaa cgcatcctcc ggcttcacca gcccgagaag gagagccgcg agcaaggcga
     1801 ggacggcgac catatcgtag cggatccgcc cccagatgaa gagcagcatc attccggcga
     1861 ggaggccgaa ggcggcaagc tgggggaggg tcgcggtcac aggttcgggc ggaacgccgg
     1921 agggggcaac ctgttgcctc cccatcgaaa gatggggagg gggaccatcc gaagggtggt
     1981 ggaggggtca ttggagcggg cctcg
//
LOCUS       DASWNU010000061         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_106219, whole genome shotgun sequence.
ACCESSION   DASWNU010000061 DASWNU010000000
VERSION     DASWNU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106219"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1285)
                     /locus_tag="VGB57_01235"
     CDS             complement(146..1285)
                     /locus_tag="VGB57_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0129996.1"
                     /translation="MNIRHRVELSETERRGLRAMLGGGRHAARKLKRAQILLAADAGV
                     PDDAIAASLAVGGSTVYRTKRRFVEGNLERALSEEPRPGAARKLSGREEALLVATACS
                     SPPEGRARWTIELLAGEMVRLTRHECLSRETVRRRLAENALKPWQRKMWCVPRIDGEY
                     VARMEDVLDLYAEAPDPKRPVVCFDESPTQLIGELRRPLPPEPGQVERYDYEYRRNGT
                     VNLFVVLDAHRPWRKVKVTERRTAADFAACMRELAEVDFPEAERIRVVMDNLSTHTPG
                     ALYEAFPAPEAHRILRRLEFHFTPKHASWLNMAEIEIGVLRHQCLDRRIGERERLEHE
                     IAAWERERNAEGARVRWMFTTEKARTKMGRAYPMAAAPDPSTKVS"
     gene            1334..1618
                     /locus_tag="VGB57_01240"
     CDS             1334..1618
                     /locus_tag="VGB57_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0129997.1"
                     /translation="MSSPGAGFTDKQGQYLAFIDAYTRVNGRPPAEADLQRHFRVSPP
                     SVHQMVLTLERAGLIRRQPGLARSIEVLVQPERLPVLHPSQPVKSSVAKY"
BASE COUNT          233 a          598 c          561 g          261 t
ORIGIN      
        1 caccctgcct gcattccccg ggagggcagg ggggctgcgt cgtccggttc tctcgaccgt
       61 ggcgtccgag gagggccaac caacaccgcc gtgacgccga ccgtcaggtc cttgccggac
      121 cggacctagt acctcggcac agagattatg acaccttggt ggatgggtcg ggagcggctg
      181 ccatcggata ggcgcggccc atcttggtgc gggccttctc ggtggtgaac atccacctga
      241 cccgggcgcc ctccgcgttg cgctcgcgct cccaggcggc gatctcgtgt tcgagccgct
      301 cgcgctcgcc gatgcgacgg tccaggcact ggtgacgcag caccccgatc tcgatctcgg
      361 ccatgttgag ccagctggcg tgcttggggg tgaagtggaa ctccagccgg cgcaggatcc
      421 ggtgcgcctc cggggccggg aaggcctcgt agagcgcccc gggcgtgtgc gtcgacaggt
      481 tgtccatcac cacgcggatc cgctcggctt ccgggaagtc gacctcggcg agctcccgca
      541 tgcaggcggc gaaatccgcc gcggtgcgcc gctccgtgac cttgaccttg cgccagggac
      601 gatgcgcgtc gaggaccacg aacaggttca ccgtcccgtt gcggcgatac tcgtagtcat
      661 aacgctccac ctgccctggc tcgggcggca gcgggcggcg cagctcgcca atgagctggg
      721 tcgggctttc atcgaagcac acgaccggcc gcttcggatc tggggcctcg gcgtagagat
      781 ccagcacgtc ctccatgcgg gccacatact cgccgtcgat ccgcgggacg caccacatct
      841 tccgctgcca tggtttcagc gcgttctcgg ccaggcggcg gcgcacggtc tcgcgcgaga
      901 ggcactcgtg ccgggtcagg cgaaccatct cgccggccag cagctcgatg gtccagcggg
      961 cgcggccttc gggcgggctc gaacaggcgg tcgccaccag caaggcctcc tcccggcccg
     1021 agagcttgcg ggcggcaccg ggacgtggct cctcgctgag cgcccgctcc aggttgccct
     1081 ccacgaagcg gcgcttggtc cggtagaccg tcgagccgcc cacggcgagg ctggcggcga
     1141 tcgcgtcgtc cggcacgccg gcatcggcgg ccagcaggat ctgcgcccgc ttcagcttgc
     1201 gggcggcgtg ccggccgccg ccgagcatcg cccgcaggcc gcgccgctcg gtctcgctca
     1261 gctcgacccg gtggcgtatg ttcatcggcg cctctcccgc gggagaggcc cagacgagtc
     1321 gaatgtgcga cggatgtcgt ctcccggggc cggcttcacc gacaagcagg gccagtactt
     1381 ggccttcatc gacgcctaca cgcgggtcaa tggccggccg ccggccgagg ccgacctgca
     1441 gcgccacttc cgcgtcagcc ccccttcggt tcaccagatg gtgctcacgc tcgagcgcgc
     1501 cggactcatc cggcggcaac ccggcctggc ccgaagcatc gaggtgctgg tgcagcccga
     1561 gcgcctgccc gtcctacacc catctcaacc cgtcaaatcc tctgtggcga agtactagat
     1621 gccgtaatgg gccggccgcc tgacgatagg cgg
//
LOCUS       DASWNU010000062         7364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_53964, whole genome shotgun sequence.
ACCESSION   DASWNU010000062 DASWNU010000000
VERSION     DASWNU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53964"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="VGB57_01245"
     CDS             complement(<1..1956)
                     /locus_tag="VGB57_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY0129998.1"
                     /translation="MTNLPPAVAQWFAAKGWAPRRHQLEMLAAARAGRSALLVAPTGA
                     GKTLAGFLASIVELIEAPSEGLHTLYISPLKALAVDVQRNLLTPIGEMDLPIRVEART
                     GDTPADRKARQRVRPPQMLLTTPESLSLLLSHEDSPTLFAGLRTVVVDEVHAFASGKR
                     GDLLSLSLARLQRLAPGMRRVALSATVSDPEAYQGWLAPHGDVELVDIVQGEPGADPD
                     LKIMLPEDERIPWSGHSGRWATANVMREIERHRTTLVFCNTRSLAELIFQDLWTANEG
                     HLPIGIHHGSLSVEARRKVESAMADGRLRGLVCTASLDLGVDWGDVDLVIQMGAPKGS
                     SRLLQRIGRANHRLDEPSKALVVPGNRFEYLEARAALDAIENKELDPEIFRPGAIDVL
                     AQHVMAAACAAPFRESEMLDEVRSAAPYAGLSEELFAQTLAYIENGGYSLRAYDRFRR
                     LTRDQDGTWRVSHPRFVQQHRMNAGIIVDAPLLDVRFRRGRKLGTVEEYFASTLSPGD
                     TFFFAGLVLEVEKIDGTDLFVRATTKSARIPTYVGTRMAMTTNLADRVRAFLWDRGQW
                     PRFPDDVRDWLQVQGERSLLPRPDQLLVETFPHEHRHYMVVYSFEGWNAHQSLGMLIT
                     RRMESAGLNPLGFVSNDYALACYALQPI"
     gene            complement(2050..2244)
                     /locus_tag="VGB57_01250"
     CDS             complement(2050..2244)
                     /locus_tag="VGB57_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0129999.1"
                     /translation="MDAGGGSWGIINFVGPLLLAAVLLWVLLRNRKARHRDVDRTEQG
                     TRDLYREEEEKRRSGDDRTS"
     gene            2319..3320
                     /locus_tag="VGB57_01255"
     CDS             2319..3320
                     /locus_tag="VGB57_01255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEY0130000.1"
                     /translation="MARLGSWIEPFPEGIYIKPADAWVDPNVPKPRVLVTHGHADHAR
                     GGHEEVFATPETLAIMECRYGPQPGGRATPYGEVIRLGEVEASFVPAGHVLGSAQIVL
                     EYRGERVVVSGDYKRRPDPTCPPFEPVPCDIFITEATFGLPVFRHPDTSSEIDRLLAR
                     LHENPERCVLVGAYALGKAQRLMMEIRGRGYHEPIYIHGALERLCDLYREWGVELGDL
                     RPATGASKDELRGKIVVAPPGCLNDRWSRRLPDPITAMASGWMRVRQRARQRNVELPL
                     IVSDHADWDELTTTIQELQPREVWITHGREDALMHWCMTRQIKARELNLVGREDEDD"
     gene            3552..4691
                     /locus_tag="VGB57_01260"
     CDS             3552..4691
                     /locus_tag="VGB57_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130001.1"
                     /translation="MAGRKSRLAWAKWLALAIGLVLLLFLILVLLAPADPARGAAPVP
                     LEQTLAYRMLERERAVREVPDSAEPLLRDVLIGAMAVLGPDPAPPATAEEFRAFGEKV
                     SIALAAHNFIQPGSPQDWPDTLGEAFAPLARNDRRVASALKADRPARLRRMKREGPVY
                     FVDCDIGAMLIISVAQMVGFDVAMVEVPGHNFIRWKSPSGAAASWDWTYWRASDEAAR
                     ASRLQSGLLTRNVFLASQTLKETEAYFKSVLAGQIDDPAAAMALRREAATTFPNNRVV
                     AYGVARTFSLSPAASPAERREALGYSLLALSMEGNGNAESVAHRMMAVACAYAANGEP
                     ALAKAFVQRARTMAEIDGGYGGSIAAVRADRTCPLERGSALQIDR"
     gene            complement(4668..4916)
                     /locus_tag="VGB57_01265"
     CDS             complement(4668..4916)
                     /locus_tag="VGB57_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130002.1"
                     /translation="MPKLYPEALLFCALWAALAIYGGLELGWKAGAVLTLGLFILIMP
                     TSAIILSRTGSFKLERGVRWAILVVAAAVLLSAVDLQG"
     gene            complement(4916..6298)
                     /locus_tag="VGB57_01270"
     CDS             complement(4916..6298)
                     /locus_tag="VGB57_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY0130003.1"
                     /translation="MSHRLPPTSLREYDIRGIYGETLGEEDARAVGRTFGTLVRREGG
                     SVVAVGRDGRVSSPGLESALIEGLTASGVDVVRIGLGPTPMLYYAEAELAVDGGIMIT
                     GSHNPGNYNGFKMVLKHGAFFGEQIQRLGRMAAAGDWEYGSGSVRDEDVMELYVERLL
                     QGFDGGPFRIGWDCGNGAAGPVVERLLKRLPGEHFTLFTEVDGRFPNHHPDPTVESNL
                     EDLKRLVREKGLDFGVAFDGDADRLGAVDGEGRVVWGDQLLSILAEPVLREVPGATII
                     ADVKASQALFDRVAELGGQPLMWKTGHSLIKSKMKETGAPLAGEMSGHIFFKHEWYGF
                     DDAIYAAVRLIRAVAQLGGSLTALRSAMPPMINTPEMRFQVDESRKFPVVEEVLDRLA
                     ADGADVDRTDGARVSTADGWWLLRASNTQDVLVARAEARDEAGLGRLVAQIDAQLALS
                     GLERGEQVAH"
     gene            complement(6326..6742)
                     /locus_tag="VGB57_01275"
     CDS             complement(6326..6742)
                     /locus_tag="VGB57_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0130004.1"
                     /translation="MGVLILLAAGGLLWAFATGRLKGFTYEDGIAAALFLLGLSLFTK
                     GRLVLGAALMATALLWAAWRRSRPSTRPMPVADARRLLGVGEGATLQEIRDAHRRLIA
                     RVHPDTGGSAELAHRVNAARDTLLAETMNRKPPRAS"
     gene            complement(6748..>7364)
                     /locus_tag="VGB57_01280"
     CDS             complement(6748..>7364)
                     /locus_tag="VGB57_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEY0130005.1"
                     /translation="AALPMPTYETFDPARNDDLNGQLDRLSEGVDVVVVDTPGRDDPY
                     ARAAMLRADTLVTPINDSFVDLDLIGEVDPETYKVRRPSFYAELVWNSRTQRAKTHGA
                     SVDWVVLRNRMQHIEARNMRRVGEALGELSRRVGFRVIPGLGERVIYRELFPKGLTLL
                     DLPHLGEVGIAHIAARQELREMIAGFALPQPGDTAAPRAAALAG"
BASE COUNT         1154 a         2442 c         2606 g         1162 t
ORIGIN      
        1 gatcggctgg agtgcgtagc aggcgagggc gtaatcgttg gagacgaagc ccaacgggtt
       61 cagccccgcg ctctccatcc gccgggtgat cagcattccg agcgactggt gggcattcca
      121 gccctcgaag ctgtagacga ccatgtagtg gcggtgctcg tgcgggaagg tctcgaccag
      181 gagctggtcc gggcggggga ggagcgagcg ctcgccctgg acctggagcc agtcgcggac
      241 gtcgtcgggg aagcgcggcc attgcccgcg gtcccacagg aacgcccgga cccggtcggc
      301 gaggttggtc gtcatcgcca ttcgggtgcc gacataggtg gggatgcgcg cggatttggt
      361 cgtggcccgg acgaacaggt cggtgccgtc gatcttctcc acctccagca ccagtccggc
      421 gaagaagaag gtgtcgcccg gcgagagggt cgaggcgaaa tattcctcga cggttccgag
      481 cttgcggccg cggcggaagc ggacgtcgag gaggggcgcg tcgacgatga tcccggcgtt
      541 catccggtgc tgctggacga agcgggggtg ggagacccgc cacgtcccgt cctgatctcg
      601 ggtgaggcgg cggaagcggt cgtaggcgcg gagcgaatag ccgccattct cgatataggc
      661 gagggtctgg gcgaacaatt cctccgacag ccccgcataa ggcgcggcgg agcggacctc
      721 gtccagcatc tccgactccc ggaagggcgc ggcgcaggcg gcggccatga cgtgctgggc
      781 gaggacgtcg atcgctccgg ggcggaaaat ctccggatcg agctccttat tctcgatcgc
      841 gtcgagtgcg gcccgggctt ccagatattc gaagcggttg ccggggacga cgagcgcctt
      901 cgaaggctcg tcgagccggt gattggccct gccgatgcgc tggaggaggc gcgacgagcc
      961 cttgggggcg cccatctgga tgacgaggtc gacgtcgccc caatcgaccc cgagatcgag
     1021 gctggcggtg cagacgaggc cgcgcagccg cccgtccgcc atcgccgact cgaccttgcg
     1081 gcgcgcctcg accgagagcg agccgtggtg gatcccgatc ggcagatgcc cctcgttggc
     1141 cgtccacaaa tcctggaaga tgagctcggc gaggcttcgg gtgttgcaga agaccagggt
     1201 tgtcctgtgc cgctcgatct cgcgcatgac gttggccgtc gcccagcgcc ccgaatggcc
     1261 ggaccagggg attcgctcgt cctccgggag catgatcttg aggtccggat cggcgcccgg
     1321 ctcgccctgg acgatgtcga ccagctccac gtcgccatgg ggcgccagcc agccctgata
     1381 ggcttcggga tcggagacgg tggcggaaag ggcgacccgg cgcatccccg gcgccaggcg
     1441 ctgaaggcgg gcgagcgaaa gggagaggag atcgccgcgc ttgccgctgg caaaggcgtg
     1501 gacctcgtcg accaccaccg tcctcaggcc ggcgaagagg gtggggctgt cctcgtggct
     1561 gagcaggagg ctgagcgatt ccggcgtggt caggagcatc tgcggcggcc gaacgcgctg
     1621 gcgggccttg cgatcggcgg gggtgtcgcc ggttcgggcc tcgacccgga tcggcaggtc
     1681 catctcgccg atcggcgtga gcaggttgcg ctggacgtcg acggcgagcg ccttcagcgg
     1741 cgagatgtag agggtgtgga ggccctcgct gggcgcttcg atcagctcca cgatcgaggc
     1801 gaggaagccc gcgagggtct tgccggcgcc ggtgggcgcg acgagcaggg cgctccgccc
     1861 ggcgcgggcg gcggcgagca tctcgagctg gtggcggcgc ggcgcccacc ccttggcggc
     1921 gaaccattgt gctacggcgg ggggaagatt cgtcattccg gacttgatcc ggaatccatg
     1981 aacactgtct tctgcgaact ctcaaccgcc gtgttcatgg atcccggcct ccgccgggat
     2041 gacgcgaagt cacgacgtgc ggtcgtcccc gctgcgccgt ttctcctcct cctcgcggta
     2101 gaggtcgcgg gtgccctgct cggtccggtc gacgtcgcgg tggcgggcct tgcggttgcg
     2161 gagcaggacc cacagcagga cggccgcgag cagcagcggc ccgacgaagt tgatgattcc
     2221 ccaactgcct ccacctgcgt ccatgctttc gctccttggc ttgccttcat tcgggcgccg
     2281 cgcttggcga tgcccgtctt caccccatat agctcccgat ggcccgtctc ggttcctgga
     2341 tcgagccttt tcccgaaggc atctacatca agccggccga cgcctgggtc gatccgaacg
     2401 tccccaagcc gcgagtcctc gtcacccacg gccatgccga ccatgcccgg ggcggtcatg
     2461 aagaggtgtt cgcgaccccc gagacgctgg cgatcatgga atgccgctac gggccgcagc
     2521 cgggcggtcg ggcgacgccc tatggcgagg tgatccggct cggcgaggtc gaggcgagct
     2581 tcgtccccgc cggccatgtg ctcggctccg cccagatcgt tctcgaatat agaggcgagc
     2641 gagtcgtcgt ctccggcgat tacaagcgcc gcccggaccc cacctgcccg cccttcgagc
     2701 cggtgccctg cgacatcttc atcaccgagg cgaccttcgg cctgcccgtc ttccgccatc
     2761 cggacacgtc gagcgagatc gaccggctgc tcgcccggct ccacgaaaat ccggagcggt
     2821 gcgtgctggt cggcgcctat gcgctgggca aggcgcagcg gctgatgatg gagatcaggg
     2881 gccgcggcta ccacgagccg atctacatcc acggcgcgct cgagcgattg tgcgacctct
     2941 accgcgaatg gggcgtggag ctgggcgacc tccgcccggc caccggcgcc tccaaggacg
     3001 agctccgcgg caagatcgtc gtcgcccctc cgggctgcct caacgaccgc tggtcgaggc
     3061 ggcttcccga tccgatcacc gccatggcct cgggctggat gcgggtccgc cagcgcgccc
     3121 ggcagcgcaa cgtcgagctg cccctcatcg tctccgacca tgccgattgg gacgagctga
     3181 ccacgaccat ccaggagctc cagccccgcg aggtctggat cacccacggc cgcgaggacg
     3241 ccttgatgca ctggtgcatg acccgccaga tcaaggcccg ggagctcaat ctggtcggcc
     3301 gcgaggacga ggacgattga agcgcctcta attcctcccc cgcctttgcg ggggaggggg
     3361 accgcgcgaa gcgcggtgga ggggttcttt cagagtcggc aagacccctc caccatgctt
     3421 cgcatggtcc cctccccagc aaatgcaggg gaggaattta ggaatccacg gccccatctg
     3481 gacgaaagcc gttgtgggcc tcgcctgagg cgaggataac tcgtcgcacg aggactgagg
     3541 gggaattgcc tatggccggt cggaaatcac gcctggcgtg ggcgaaatgg ctcgccctgg
     3601 cgatcggcct cgtccttctg cttttcctga ttcttgtgct tcttgcgccg gctgacccgg
     3661 cgcggggggc ggcgcccgtt ccgctcgagc agacccttgc ctaccggatg ctcgagcggg
     3721 agcgggcggt cagggaagta ccggactcgg ccgagccgct gctgcgcgac gtcctcatcg
     3781 gcgcgatggc ggtgctcggg ccggaccccg ctccgcccgc gaccgcggaa gagttcaggg
     3841 ccttcggcga aaaggtctcg atcgccctcg ccgcccataa tttcatccag cccggctctc
     3901 cccaagattg gccggacaca ttgggggagg cgttcgcccc ccttgcccgc aacgaccgcc
     3961 gggtcgcttc cgcgctcaag gccgatcggc cggccaggct gcgccgcatg aagagggagg
     4021 gtccggttta tttcgtcgac tgcgacatcg gcgcaatgct gatcatcagc gtcgcccaga
     4081 tggtcgggtt cgacgtggcg atggtcgagg tgcccggcca caatttcatc cgctggaaat
     4141 caccctccgg tgcggccgcg agctgggact ggacctattg gcgcgcatcg gacgaggcgg
     4201 cacgggcgtc gcgccttcag agcgggctgc tcactcgaaa cgtcttcctc gcctcgcaaa
     4261 ccctcaagga gacggaggcc tatttcaaaa gcgtcctggc cggccagatc gacgatccgg
     4321 cggcggcgat ggcccttcgc cgggaggccg cgacgacctt ccccaacaat cgcgtggtcg
     4381 cctacggagt cgcccgcacc ttttcactca gcccggcggc ctcgcccgcc gagcggaggg
     4441 aggcgctcgg ctacagcctg ctggcgctgt cgatggaggg gaacggcaat gccgaaagcg
     4501 tcgcccacag gatgatggcc gtcgcctgcg cctatgccgc caacggtgag ccggccctcg
     4561 cgaaggcgtt cgtccaaagg gcgcggacga tggccgaaat cgacggggga tatggcggga
     4621 gcatcgccgc cgttcgagcg gatcgaacct gcccccttga gcgaggctca gccttgcaga
     4681 tcgaccgctg acaggagcac tgcggccgcc acgaccagga tggcccaccg cacgccgcgc
     4741 tccagcttga agctgccggt gcgcgagagg atgatcgcgc tggtcggcat gatcaggatg
     4801 aacaggccga gggtgagcac ggccccggcc ttccacccga gctcgagccc gccataaatg
     4861 gcgagggcgg cccagagggc gcagaagagc agcgcctccg ggtagagctt cggcatcagt
     4921 gggcgacctg ctcgccgcgt tcgaggccgg acaaggcgag ctgggcgtcg atctgggcga
     4981 cgaggcggcc gaggccggct tcgtccctgg cctcggctcg ggcgacgagg acgtcctggg
     5041 tgttggaagc gcgaagcagc caccagccgt cggcggtgct gacccgggcg ccgtcggtgc
     5101 ggtcgacgtc ggcgccatcc gcggcgaggc ggtcgagcac ctcctcgacg accgggaact
     5161 tgcggctctc gtcgacctgg aagcgcattt cgggcgtgtt gatcatcggc ggcatcgccg
     5221 agcgcagcgc ggtcagcgag ccgccgagct gggcgacggc tcggatcagg cggacggcgg
     5281 cgtagatggc gtcgtcgaag ccgtaccatt cgtgcttgaa gaagatgtgg ccgctcatct
     5341 cgccggcgag gggagcgccc gtctccttca tcttggactt gatcaggctg tggccggtct
     5401 tccacatcaa gggctgcccg ccgagctcgg cgacccggtc gaaaagggcc tggctcgcct
     5461 tgacgtcggc gatgatggtg gcgcccggca cctcgcgaag caccggctcg gcgaggatcg
     5521 agagcaattg gtcgccccag acgacccggc cttcgccgtc gaccgcgccg aggcggtcgg
     5581 catcgccgtc gaaggcgact ccgaaatcga gccccttctc gcgcaccagc cgcttcagat
     5641 cctcgaggtt ggattcgacc gtgggatcgg gatgatggtt ggggaagcgg ccgtcgacct
     5701 cggtgaagag ggtgaaatgc tcgcccggca gccttttcag cagccgctcg acgaccggcc
     5761 cggcggcgcc gttgccgcaa tcccagccga tccggaacgg gccgccgtcg aagccttgca
     5821 gcagccgctc gacataaagc tccatcacat cctcgtcgcg gacggagccg gagccatatt
     5881 cccaatctcc ggccgcggcc atccggccga gcctctggat ctgctcgccg aagaaggcgc
     5941 catgcttcag caccatcttg aagccgttgt aattgccagg attgtggctt ccggtgatca
     6001 tgattccgcc gtcgacggca agctccgctt cggcgtaata aagcatcggg gtcggaccga
     6061 gtccgatccg gacgacgtcg accccgctcg cggtcaggcc ctcgatcagc gccgattcca
     6121 gccccggcga gctgacccgg ccgtcgcggc cgaccgccac gacgctcccg ccttcgcggc
     6181 ggacgagggt gccgaaggtg cggccgaccg ctctcgcatc ctcctcgccc agcgtctctc
     6241 cgtagattcc cctgatgtca tattcgcgca gagaggtcgg cggcaggcga tggctcattc
     6301 gggactccgg ctaaggggga ttcgatcaag acgcgcgagg cggcttgcgg ttcattgttt
     6361 cggcgagcag cgtgtcgcgg gcggcgttga cccggtgggc gagctcggcc gagccgcccg
     6421 tgtcgggatg gaccctcgcg atcagccggc ggtgggcgtc gcgaatctcc tggagggtgg
     6481 cgccttcgcc gactccgagc agcctgcggg cgtcggcaac cggcatcggc ctcgtcgacg
     6541 gtcgcgagcg gcgccaggcg gcccagagca gggcggtcgc catcagggcc gcgccgagga
     6601 cgagcctgcc cttggtgaac aggctgaggc cgagcaggaa gagggcggcg gcgatcccgt
     6661 cctcgtaggt gaagcccttg agccggccgg tggcgaaagc ccagagcagg ccgccggccg
     6721 cgagcaggat gagaacgccc atcccggcta gccggcgagc gcggccgccc ggggagcggc
     6781 cgtatcgccc ggctggggca gggcgaagcc ggcgatcatc tcgcgcaatt cctggcgggc
     6841 ggcgatatgg gcgatcccga cctcgccgag atggggcaga tcgagcaggg tcaggccttt
     6901 ggggaagagc tcgcggtaga tgacgcgctc gcccaggccc ggaatgaccc ggaagccgac
     6961 gcggcgcgaa agctcgccga gcgcctcgcc gacgcggcgc atgttgcggg cctcgatatg
     7021 ctgcatccgg ttcctgagca cgacccagtc gacgcttgcg ccgtgggtct tggcgcgctg
     7081 ggtgcggctg ttccagacca gctccgcgta gaaacttgga cggcgcacct tgtacgtctc
     7141 ggggtcgact tcaccgatca gatcgagatc gacgaagctg tcgttgatgg gagtgaccaa
     7201 agtgtccgcg cggagcatgg cggcgcgcgc ataaggatcg tcgcggccgg gcgtgtccac
     7261 cacgacgaca tcgacgcctt cggaaagccg gtccagctgg ccgttgagat catcgttcct
     7321 ggcggggtcg aacgtctcgt aggtcggcat ggggagggca gcgt
//
LOCUS       DASWNU010000063         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_210227, whole genome shotgun sequence.
ACCESSION   DASWNU010000063 DASWNU010000000
VERSION     DASWNU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210227"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGB57_01285"
     CDS             <1..685
                     /locus_tag="VGB57_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130006.1"
                     /translation="IRNVDIEKIDGVAPAGGICLEPDLSAYPNRNIRISNSRIRNVQV
                     GVYVTSANQGVSIAGMDIEAMNSGVIVADNSSDIRIENNPNIKSLVGGAEGGAIRTVV
                     TRAETVRGLTIRNNQLSGGGFFVIDIYGPGYQGLVVSGNRISATNAGTQGIVRIGAGQ
                     FLDNICVIGPRAGIKSQYFVHLQGVTYGRNSYRNDSPHPMYSAFRGGRDIGGDRFLSK
                     SLVHHWEPI"
     gene            755..1090
                     /locus_tag="VGB57_01290"
     CDS             755..1090
                     /locus_tag="VGB57_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130007.1"
                     /translation="MDWEQVKNAIAIWTELERDALHIYAAVLLQIGSAALLRRSLASP
                     VPWLVVLAAALGNEALDMRRDGLFEDWERAAALHDLWNSMLVPTILLLLVRFAPRLVT
                     RPAKPPPSR"
     gene            complement(1082..2515)
                     /locus_tag="VGB57_01295"
     CDS             complement(1082..2515)
                     /locus_tag="VGB57_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0130008.1"
                     /translation="MLRAPSPALLDSLESLLGPRGFTRDADALEPWLSDWRGRYRGRA
                     AALLSPASAAEVQAIVGRCAEARVGLVPQGGNTSMVGGATPGAEGDSLLLSLRRMNAV
                     RSVSREDNVAVAEAGVILSDLHDWAAAAGRRFPLSLGAKGSATVGGLVSTNAGGTQVL
                     RFGPMRSLVVGLEAVLPDGSLLEGLSALRKDNRGYDLKQLLIGAEGTLGIVTAASLKL
                     VAAPGSRSVAWVGLASPAAALTLLRRLEARLGEAVESFELVPADALGLVLAGIAGTRP
                     PLAGAHRWHVLVEAIAPEGGRDASEALQDALAAALDEGLAEDATVAASEAQAAALWRL
                     RESIAEAERLQGPSVKHDVSVPVSAMPAFIEEARAAVEARFEGARVVAFGHLGDGNVH
                     FNVQPPAGIAADAWLAAEAGAVTRLVHDRVGEAGGSISAEHGIGQSKLAELARTAQPA
                     RLAALRAVKQALDPAGIMNPGKLLPSS"
BASE COUNT          426 a          972 c          863 g          357 t
ORIGIN      
        1 gatcaggaac gtcgacatcg agaagatcga cggggtggct ccggccggag gaatctgtct
       61 cgagccggat ctgtcggctt accccaaccg caatatccgc atttccaaca gccggatccg
      121 caacgtccag gtcggagtct acgtcaccag cgccaatcag ggggtctcga tcgccggaat
      181 ggacatcgag gcgatgaaca gcggggtcat cgtcgccgac aacagctccg acatccgaat
      241 cgagaacaat cccaacatca agtccctggt cggcggagcc gaagggggcg cgatccggac
      301 cgtggtcacc cgcgccgaga cggtcagggg cctgacgatc cgcaacaacc agctttccgg
      361 cggcggcttc ttcgtcatcg acatatacgg gcccggctat caggggctcg tcgtcagcgg
      421 caaccgcatc tcggcgacca atgccggaac ccagggtatc gtccgcatcg gggcgggcca
      481 gttcttggac aatatctgcg tgatcggccc tcgcgccgga atcaagagcc agtatttcgt
      541 ccacctccag ggcgtgacct acgggcgcaa cagctaccgc aacgattccc cgcaccccat
      601 gtattccgcc ttccgcggcg gccgcgacat cggcggcgac cgcttcctga gcaagtcgtt
      661 ggtccaccat tgggagccca tataaggtcg ctcgtccctc tcccgtgaaa gcgggaaggg
      721 gtcgaatcga agcgggcagc aagaggaact ccggttggac tgggagcagg tcaagaacgc
      781 catcgcgatt tggaccgagc tcgagcgcga cgccctccac atctatgcgg ccgtcctcct
      841 ccagatcggc tcggcggcct tgctgaggag gagcctggcc tcgcccgtcc catggctcgt
      901 ggtgctcgcc gccgcgctgg ggaacgaggc cctcgacatg cgtcgcgacg ggctgttcga
      961 ggattgggag cgggcggcgg cgctccacga tctgtggaat tcgatgctgg tgccgacgat
     1021 cctgcttctg ctcgtccgct tcgcgccgcg cctcgtcacc cggccggcca agccgccgcc
     1081 ttcacgatga gggaagcagc tttcccggat tcatgatccc ggccgggtcg agcgcctgct
     1141 tgaccgcccg aagcgccgcc agccgggccg gctgggcggt tcgggcgagc tcggcgagct
     1201 tggactggcc gatgccgtgc tcggccgaga tcgagccgcc ggcctccccg acccggtcgt
     1261 ggaccagccg ggtcacagcg cccgcctccg cggcaagcca cgcatcggcg gcgatcccgg
     1321 ccggcggctg gacgttgaaa tggacgttgc cgtcgccgag atggccgaac gccaccaccc
     1381 gggcgccttc gaaccgggcc tccaccgccg cccgcgcctc ctcgatgaag gcgggcatgg
     1441 cggaaaccgg caccgagacg tcgtgcttca ccgacggccc ctgcagccgc tccgcctcgg
     1501 cgatcgattc gcgaagccgc cacaaggccg ccgcctgggc ctcgctcgcg gcgaccgtcg
     1561 cgtcctcggc cagcccctcg tccagagccg cggcgagcgc gtcctgaagc gcctccgagg
     1621 cgtcgcgccc gccctccggg gcgatggctt cgacgaggac gtgccagcga tgggcacccg
     1681 ccagcggcgg ccgggtcccg gcgatcccgg cgaggaccag gccgagcgcg tccgccggca
     1741 ccagctcgaa gctctcgacc gcctcgccga gccgcgcctc gagccggcga agcagggtga
     1801 gcgccgccgc gggcgaagcg agcccgaccc aggccaccga gcgcgagccc ggcgcggcga
     1861 ccagcttcag gctggcggcg gtgacgatcc cgagcgttcc ttccgctccg atcaggagct
     1921 gcttcagatc gtagccgcga ttgtccttgc ggagcgccga cagcccttcg agcagggacc
     1981 cgtcgggaag caccgcctcg agcccgacga cgagcgagcg catcggcccg aagcgaagca
     2041 cctgggtccc gccggcattg gtcgagacga gcccgcccac cgtcgccgag cccttggcgc
     2101 cgagcgagag cgggaagcgc cgtccggccg ccgccgccca atcgtgcagg tcggagagga
     2161 tcaccccggc ttcggccacc gcgacattgt cctcgcggga gacgctccgc accgcgttca
     2221 tccggcgcag cgacagcagc agcgagtcgc cctccgcccc gggcgtcgcg ccgccgacca
     2281 tcgaggtgtt gccgccctgc ggcaccagcc ccacccgggc ttcggcgcag cgcccgacga
     2341 tcgcctgcac ctcggccgcc gacgcgggcg aaagcagcgc cgccgcccgc ccccggtacc
     2401 gcccccgcca gtcgctcagc cacggctcca gcgcgtccgc atcgcgcgtg aagccgcgcg
     2461 gccccagcag ggattccagc gagtcgagca aagcgggcga aggcgctcgg agcatcgccc
     2521 ttccctgacc gacctgacgc ccgcaatcaa tcgccgggcc aggcacgcct gtatgattcg
     2581 cttacaatgt caaagagcct cggttggaac atagcaag
//
LOCUS       DASWNU010000064         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_287789, whole genome shotgun sequence.
ACCESSION   DASWNU010000064 DASWNU010000000
VERSION     DASWNU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287789"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGB57_01300"
     CDS             complement(<1..364)
                     /locus_tag="VGB57_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0130009.1"
                     /translation="MARGGETRGISRRSLLVGGGAGAGLLLAWALWPRSFEHNLRAGE
                     GEQAFNAFLKIGNDGRVLVAVPQAELGQGSWTALAQILADELGADWRTVGVEPAPIGP
                     IYANRLLAEGEEDASFEGG"
     gene            complement(538..2217)
                     /locus_tag="VGB57_01305"
     CDS             complement(538..2217)
                     /locus_tag="VGB57_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY0130010.1"
                     /translation="MPSELDRKFDAVLAQVTGPGGRIQIAEDSQGRKIVANLPPTLPG
                     MFDAFCMLHGATTAVIADGERLTFAELNAQATGLARALAGGWGVGKGDRVAIAMRNCP
                     SWIVAYMAVLKAGGVATLVNGWWQSDELRHGLALTEPCLVLCDAPRAKRLEATGLGIA
                     SVVLPVEKPIAEALAPLLGRGEADLPEVTPEDDATILFTSGSTGAAKGAVSNHRAVTT
                     GIYTYSISLLALLGIMESEGRGPANPPRTLVNVPLFHVTGEVPVLLNSFVIGRGMVLM
                     AKWDPGEALRLIQDEKVTYFVGVPTMSLELMQHPDRERYDLSSLTDIAAGGAPRPVAH
                     VKRLQESFKGAQPALGYGLTETNAVGCGNFWQNYADKPASTGRPHKPTVELAILGEGD
                     RHLPVGETGEIAIRSAANFRGYWNDAASTAAAFAADGFLKTGDIGYLDEEDYLFIVDR
                     KKDIIIRGGENISCQEVEAAIYSHPAVSEASVFGVPCERLGEVPAAVIYSEDDRALGA
                     EALRDFLDGRIASYKIPQHIWVHDEPLPKLGTGKIDKKTLRERYSAEVEKA"
     gene            2313..3155
                     /gene="lgt"
                     /locus_tag="VGB57_01310"
     CDS             2313..3155
                     /gene="lgt"
                     /locus_tag="VGB57_01310"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0130011.1"
                     /translation="MAAGDFVRFEDLGLSPTLFDWPFPLRWYSLGYLAGILIGWWYLM
                     KLIDRPGAPMARRHADDMVFYATLGIILGGRLGYVLFYRPAFYFADPIQIVRLWDGGM
                     SFHGGVIGTTIGILWMARKHKLDWLRVHDYVACCAPFGIFFVRLANFVNGELWGRPTD
                     VPWAIVFPTGGEVPRHPSQLYEAGLEGILLFLVLWFLFWKTDARYHPGKLVGAFLLVY
                     GLSRFLVELVRQPDQGLENLPWGLTMGQTLTVPMVLGGAWLIATAKGRRQRVEPVAGT
                     ESVA"
     gene            3275..>4239
                     /locus_tag="VGB57_01315"
     CDS             3275..>4239
                     /locus_tag="VGB57_01315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0130012.1"
                     /translation="MSLAQRLRRLIEAQGPIPVSSYMALANSHYYATRDPLGASGDFT
                     TAPEISQMFGELIGLALADVWARDGRPEGVRYVELGPGRGTLAADALRAMRSAGLEPR
                     VHFVESSPVLRAAQAERVPHASWHEDLGTLPEDGPILVVANEFFDALPIRQLIATGAG
                     WRERVVTVDRDRFVPAAGPPVPAAAIADHVRPAPPGTVLETSPASLAYARGLANRIIM
                     HGGAALIVDYGHDRLSAGETLQAVRMHEYADPWIEPGESDLTAHVDFESLARAAAEAG
                     VSVHGPVAQGDWLARLGIDARADALARSAPDRGEALRIARDRLVSP"
BASE COUNT          613 a         1522 c         1400 g          704 t
ORIGIN      
        1 ttccgccctc gaacgatgcg tcctcctcgc cttcggcgag cagccggttg gcatagatcg
       61 gcccgatcgg cgccggctcc accccgacgg tgcgccagtc ggcgccgagc tcgtcggcga
      121 ggatctgggc cagagcggtc cagctcccct ggccaagctc cgcctgcggc acggcgacca
      181 gaacccgtcc gtcattgccg atcttgagga aggcgttgaa cgcctgctcg ccttcgccgg
      241 cgcgcagatt gtgctcgaag gagcgcggcc acagggccca ggcgaggagc aggccggccc
      301 cggcgccccc gccgaccagc agtgaccgcc ggctgatccc cctcgtctcg ccgcctcgcg
      361 ccatgggagg gctgatagag ggggaggggg cgaggggcta gcctccctaa attcctcccc
      421 ggcttttgcc ggggaggggg accaggcaaa gcctggtgga ggggttctgc tgagtccgaa
      481 gagcccctcc accgcgctcc gcgcggtccc cctccccgcg aaatcgcggg gaggaattca
      541 agctttctcc acctccgcgg aatatcgctc ccgaagcgtc ttcttgtcga tcttgcccgt
      601 gcccagcttg ggcaagggct cgtcgtgaac ccagatatgc tgcgggatct tgtaggaggc
      661 gatccggccg tccaggaaat cgcgaagcgc ctccgcgccc agggcgcggt cgtcctcgga
      721 atagatcacc gccgcgggca cctcgccgag ccgctcgcac ggcaccccga acacggaggc
      781 ctccgacacc gccgggtgcg aatagatcgc cgcctccact tcctgacagc tgatattctc
      841 gccgccgcgg atgatgatgt ccttcttgcg gtcgacgatg aagagataat cctcctcgtc
      901 gagatagccg atgtcgccgg tcttgagaaa accgtcggcc gcgaacgcgg cggccgtcga
      961 agccgcgtcg ttccaatagc cgcggaaatt ggcggccgat cggatcgcga tctccccggt
     1021 ctcgcccacc ggcagatggc ggtcgccctc gcccaatatg gcaagctcga cggtcggctt
     1081 gtgcggccgt ccggtcgagg cgggcttgtc ggcgtaattc tgccagaaat tgccgcagcc
     1141 caccgcattg gtctcggtga ggccgtagcc caaagcgggc tgcgccccct tgaagctctc
     1201 ctgcagcctc ttgacgtggg cgacgggccg aggcgctccg ccggcggcga tgtcggtcag
     1261 cgacgacaga tcgtatctct cgcgatcggg atgctgcatc agctcgaggc tcatcgtcgg
     1321 aaccccgacg aaataggtga ccttctcgtc ctggatcagc ctcagcgcct cgcccggatc
     1381 ccatttcgcc atcagcacca tgcctcggcc gatgacgaag ctgttcagga gcaccggcac
     1441 ctcgccggtg acgtggaaca gcggcacgtt gacgagggtg cgcggcgggt tggccgggcc
     1501 ccggccctcg ctctccatga tccccaggag ggcgagcagg ctgatcgaat aggtatagat
     1561 gccggtggtg accgcccggt ggttggacac ggcgcccttg gcggcgccgg tcgagcccga
     1621 ggtgaagagg atggtcgcgt cgtcctcggg agtcacttcc ggaaggtccg cctcgccgcg
     1681 cccgagcagg ggcgccagcg cctcggcgat cggcttctcg accggcagca ccacgctggc
     1741 gatgccgagg ccggtcgcct ccagccgctt cgcccgaggc gcgtcgcaca ggacgaggca
     1801 gggctcggtc aaggcgaggc cgtggcgcaa ttcgtccgac tgccaccagc cgttgacgag
     1861 agtggccacc ccgcccgcct tcagcaccgc catataggcg acgatccagg acgggcagtt
     1921 gcgcatcgcg atcgccaccc ggtcgccctt gccgacgccc cagccccccg ccagagcccg
     1981 cgccagtccg gtcgcctgag cgttcagttc ggcgaaggtc agccgctcgc cgtcggcgat
     2041 gacggcggtc gtcgccccgt gcagcatgca gaaggcgtcg aacatcccgg ggagggtcgg
     2101 gggcaggttg gcgacgatct tgcgcccctg ggaatcctcg gcgatctgga ttcggccgcc
     2161 gggcccggtc acctgcgcca gcaccgcgtc gaacttgcgg tccagctcgc tcggcatccg
     2221 catctccctt tccgtttctt cttccccctc ctattagggc ctggagcggg agggggaagc
     2281 ctttgatcga tcctttactg acaagcctgt cgatggctgc gggcgatttc gtccggttcg
     2341 aggacctcgg gctcagcccg acgctgttcg actggccctt tccgctgcgc tggtattcgc
     2401 tgggctatct cgccgggatc ctgatcggct ggtggtatct gatgaagctg atcgaccggc
     2461 ccggcgctcc catggcgcgg cgccacgccg acgacatggt cttctacgcc actctgggca
     2521 tcatcctcgg cggccgcctc ggctacgtgc ttttctaccg gcccgccttc tacttcgcgg
     2581 acccgatcca gatcgtccgc ctgtgggacg ggggaatgtc cttccacggc ggagtcatcg
     2641 gcacgacgat cggcatcctg tggatggcgc gcaagcacaa gctcgactgg ctcagggtcc
     2701 acgattacgt cgcctgctgc gcgccgttcg ggattttctt cgtccgcctc gccaatttcg
     2761 tgaacgggga attgtggggc cggccgaccg acgtcccctg ggcgatcgtc ttcccgaccg
     2821 gcggcgaggt tccgcgccac ccgagccagc tctacgaggc tgggctggag gggatcctgc
     2881 tcttcctcgt cctctggttc ctgttctgga agaccgacgc ccgctaccac ccgggcaagc
     2941 tcgtcggcgc cttcctcctc gtctacggcc tcagccgctt cctcgtcgag ctcgtccgcc
     3001 agccggacca gggcctcgag aatttgccct ggggcctcac catgggccag accctgaccg
     3061 ttccgatggt gctcggcggc gcctggctga tcgccacggc caaggggagg aggcaaaggg
     3121 tggagccggt cgcggggact gagagcgtgg cctgaaatgc cctcgccctc ccaccgcttc
     3181 gcggcgggcc actccgccgc cgatcttccc cctctccccc caggggagag ggaggggccc
     3241 gcctgccgca ggcaggtggg agggtgaggg cactatgtcg ctcgcccaac gtctgcgccg
     3301 cctgatcgag gcccagggcc caatcccggt ctccagttac atggccctcg ccaattccca
     3361 ttattacgcg acccgagacc cgctcggcgc gagcggcgac ttcaccaccg cgcccgagat
     3421 cagccagatg ttcggcgagc tgatcggcct cgcccttgcc gacgtctggg cccgcgacgg
     3481 ccggcccgaa ggagtccgct acgtcgagct cgggcccggc cgaggcaccc tcgccgccga
     3541 cgcactccgg gccatgcgct ccgccgggct cgagccccgg gtccatttcg tcgagagcag
     3601 cccggtcctg cgcgccgccc aggcggagcg ggtcccccac gccagctggc acgaggatct
     3661 cggtaccctg cccgaagacg ggccgattct ggtcgtcgcc aacgaattct tcgacgccct
     3721 cccaatccgc cagctgatcg cgaccggggc cggctggcgc gagcgagtcg tcaccgtcga
     3781 ccgtgaccgc ttcgtcccgg ccgccgggcc tccggtgccg gccgcggcca tcgccgacca
     3841 cgtccgcccc gctccgccgg ggacggtgct ggaaacctcg ccggcttcgc tggcttatgc
     3901 gcgcgggctc gccaaccgca taattatgca cggaggcgcg gcgctgatcg tcgattacgg
     3961 ccatgatcgc ctctcggcgg gcgagacgtt gcaggccgtg cggatgcatg aatatgcaga
     4021 tccgtggata gagccggggg agtcggacct caccgcccat gtcgacttcg aatcgctcgc
     4081 tcgggcagcc gccgaggcag gcgtgagcgt ccacgggccg gtcgcgcagg gcgactggtt
     4141 ggcgaggctc ggcatcgatg cccgggcgga cgccctcgcc aggtccgcac ccgatcgagg
     4201 cgaggcgctg aggatcgcaa gggaccgcct cgtctcgcc
//
LOCUS       DASWNU010000065         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_184751, whole genome shotgun sequence.
ACCESSION   DASWNU010000065 DASWNU010000000
VERSION     DASWNU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184751"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VGB57_01320"
     CDS             complement(<1..623)
                     /locus_tag="VGB57_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEY0130013.1"
                     /translation="MPQIALPLDWPVADRDEDFLISESNRGAFEHLKRWSLWPVMATL
                     VTGPRKSGRSLMGRLFVRKTGGRLFDNAEDHDEEALFHAWNEAQLSRRPLLIVADSPP
                     PLWQVALPDLRSRLAATPHVAIHGPDDRLLGDLIVKLLGDRGIAVPPEVPAFIVPRIE
                     RSYVAVLQVVAALDRAALSHHRRMTVAMVKRALSEDGMIKRARSQPE"
     gene            complement(625..1863)
                     /locus_tag="VGB57_01325"
     CDS             complement(625..1863)
                     /locus_tag="VGB57_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY0130014.1"
                     /translation="MKPSFRLSLLLVALLASLVAGRAVWAQLEGADRGVPPIDSASTF
                     EITGVEVDVSADTADKAREEGWRQAQQKGWKALWAKTNGRPIEQAPGLTDAALNGMVA
                     GIVVEQEQIGPKRYIATLGVLFDRARTGQLLGVSGSVRRSAPVLVIPVMLTGSSFQTF
                     ESRNEWQRAWARYRTGNSPVDYVRPTGSGIDPLLLNVTQSRRPGRGWWRMLLDQYGAA
                     DVITPEVHLKRLFPGGPAVGVFTARHGPDNRVLGQFTLRVENSALIPRLLDEGVRGLD
                     LLYSRALAAGQLTPDPSLIVPEPPLIEEIVEEEETLVEEAAARPLPPPGTAPVGAVTV
                     LRVQVDTPDQAAIGRAELAVSRVPGVTSAFTTNPAIGGTSSMRVSYMGDPAALAAALE
                     AQGWSVSGSGNNLRLSRGGD"
     gene            2131..3219
                     /gene="purM"
                     /locus_tag="VGB57_01330"
     CDS             2131..3219
                     /gene="purM"
                     /locus_tag="VGB57_01330"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY0130015.1"
                     /translation="MGDSYTYAKAGVSIAAGNALVKAIAPLAKSTARPGADASLGGFG
                     GFFDLKAAGYRDPLLVAANDGVGTKLKLAIDHDRHEGVGVDLVAMCVNDLIVQGAEPL
                     FFLDYFATGRLEGGIAERVIASIAEGCRQAGCALIGGETAEMPGMYAEGDYDLAGFCV
                     GAVERDEALTGARVAAGDVILGLASSGVHSNGFSLVRRLAADRGWKLERPALFDPEVL
                     LIEALLAPTRIYVRSLLPLVRAGKVRAMAHITGGGLLENVPRVLADGLHARIEAGAWP
                     QPRLMAFLQAQGNIEPEEMARTFNCGIGMALIVSQDDVKGVSQVLEEAGETVFRIGRV
                     DEGPKGCTVAGPAGSWSARGDWTATHHG"
     gene            3212..3823
                     /gene="purN"
                     /locus_tag="VGB57_01335"
     CDS             3212..3823
                     /gene="purN"
                     /locus_tag="VGB57_01335"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY0130016.1"
                     /translation="MAERVRVGVLVSGRGTNMVALSEHKRREARSYDVALVASNVPEA
                     RGLVVARRLGIPVWARSHKGLEREEFDHLLDLALREHDVEMVALAGYMRLLSGEFIRK
                     WPGRILNIHPSLLPAYKGLDTHRRALEAGEIWVGCSVHLVTEELDGGPVLAQARVRVR
                     ERETAQQLAERVLREEHRLYPETLDKYCARLRPGRVEVLPPHR"
BASE COUNT          617 a         1278 c         1438 g          603 t
ORIGIN      
        1 cattccggct ggcttctcgc cctcttgatc atgccgtctt ccgagagggc tcgcttcacc
       61 attgccacgg tcatgcgacg atgatgtgac aaagcggcgc ggtcgagcgc ggcgacgacc
      121 tgcagaacgg ccacatagga acgttcgatc cggggcacaa tgaaagcggg gacttcgggc
      181 gggacggcga tgccgcggtc gccgagcagc ttgacgatga gatcgccgag cagccggtcg
      241 tcggggccgt ggatcgcgac gtggggagtc gccgcgagtc gcgaacgaag gtccgggagg
      301 gcgacctgcc agaggggcgg cggggagtcg gcgacgatga gcagcggccg gcgcgacagc
      361 tgggcctcgt tccaggcgtg gaagagcgct tcctcgtcat gatcctcggc attgtcgaac
      421 aatcggccgc cggtcttgcg gacgaacagc cggcccatca ggctccgccc ggacttgcgc
      481 ggcccggtga cgagggtcgc catgaccggc cagagcgacc agcgtttgag atgctcgaat
      541 gcgccccggt tggactcgga gatgaggaaa tcctcatcgc ggtcggctac cggccagtcg
      601 agcggcagcg cgatctgcgg cacgtcagtc gccgccccgc gacaggcgca ggttgttgcc
      661 cgatccggag acgctccagc cttgcgcctc gagcgcggcg gcgagcgccg cgggatcgcc
      721 catgtagctc acccgcatcg acgacgtgcc gccgatcgcc ggattggtcg tgaacgccga
      781 agtcactccg ggaacccggc tgaccgccag ctccgcccgg ccgatcgcgg cctgatccgg
      841 cgtgtcgacc tgaactcgaa ggacggtcac cgcgccgacg ggagcggtgc cgggcggggg
      901 cagcggccgg gccgccgcct cctcaaccag ggtctcctct tcctctacga tctcctcgat
      961 cagcgggggc tcggggacga tcagggaagg atcgggagtc agctggccgg cggcgagagc
     1021 ccggctgtag aggaggtcga gtccgcgcac gccctcgtcg aggagccgcg ggatcagggc
     1081 gctgttctcg acccggagcg tgaactggcc gaggaccctg ttgtccggac cgtggcgggc
     1141 cgtgaagacc ccgaccgccg gcccgcccgg gaagaggcgc ttgagatgta cttcgggcgt
     1201 gatcacgtcg gccgccccat attggtcgag cagcatcctc caccagcccc ggccgggccg
     1261 gcggctctgg gtgacgttga ggaggagcgg atcgatgccc gagcccgtcg gccggacata
     1321 atcgaccggg ctgttgccgg tgcggtagcg agcccaggcc cgctgccatt cgttgcgcga
     1381 ttcgaaggtc tggaaggaag atccggtcag catcaccgga atgacgagga ccggcgccga
     1441 gcggcggacc gagccgctga ccccgagcaa ctggccggtg cgggcgcggt cgaaaagcac
     1501 tccgagcgtg gcgatgtagc gtttcgggcc gatctgctcc tgctcgacga cgattcccgc
     1561 gaccattccg ttcaaggcgg cgtcggtcag gccgggggcc tgctcgatgg ggcggccgtt
     1621 ggtcttggcc cagagcgcct tccagccctt ctgctgggcc tgacgccagc cctcctcgcg
     1681 cgccttgtcg gcggtgtcgg cgctgacgtc gacctcgacc ccggtgatct cgaaggtgct
     1741 ggcgctgtcg atcggcggaa cgccgcggtc ggcgccttcc agttgggccc agaccgcccg
     1801 gcccgccacg agggaggcaa ggagggcgac gagcagcagg gacagacgaa agcttggctt
     1861 cacgcccgcc ttttggcgag ttggacgtgg aaatccaagc gcgatgtggc cggcggccca
     1921 gcgcttctaa aatcctcccc tgcatttgca ggggaggggg accatgcgaa gcatggtgga
     1981 ggggccttac ggagtccgaa gaacccctcc accgcgcttc gcgcggtccc cctcccccgg
     2041 aaagccgggg gaggaattag gagggaacgg gccgcggggg cttggaacaa agggcttcaa
     2101 tatcggcggc acaggctcta gtcggcggcg atgggcgaca gctacactta tgccaaagcc
     2161 ggcgtttcga tcgccgccgg caacgccctg gtcaaggcca tcgcaccgct cgccaagtcg
     2221 accgcgcggc cgggggccga cgcctcgctg ggcggcttcg gcggcttctt cgacctcaag
     2281 gcggcgggct atcgcgatcc gcttctggtc gcggccaatg acggcgtcgg caccaaattg
     2341 aagctggcca tcgaccatga ccggcacgaa ggggtcgggg tcgacctcgt cgcgatgtgc
     2401 gtcaacgacc tcatcgtcca gggcgcggag ccgctcttct tcctcgatta tttcgcgacc
     2461 gggcggctcg aaggcggaat cgccgagcgg gtgatcgctt cgatcgccga aggctgccgc
     2521 caggccggct gcgccctgat cggcggcgag acggcggaga tgccgggcat gtatgccgag
     2581 ggggattacg atctcgccgg cttctgcgtc ggagcggtgg aacgggacga ggcgcttacc
     2641 ggggcaaggg tggcggccgg agacgtgatc ctcggcctcg cttcctccgg ggtccattcg
     2701 aacggcttct cgctggtgcg ccgactcgcc gcggaccggg gctggaagct ggagaggccg
     2761 gccctgttcg acccggaggt gcttctgatc gaggcgctgc tcgctccgac ccggatctac
     2821 gtgaggtcgc tgctccccct ggtccgcgcc ggcaaggtgc gggccatggc ccatatcacc
     2881 ggcggcgggc tgctggagaa cgtccctcgg gtactggccg acggcctgca cgcccggatc
     2941 gaagccggcg cctggccgca gccgcggctg atggccttcc tccaggccca gggcaatatc
     3001 gagcccgagg agatggcgcg gaccttcaat tgcgggatcg ggatggccct gatcgtgtcg
     3061 caagacgatg tgaaaggcgt ttctcaggtc cttgaagagg ctggagaaac tgttttccgc
     3121 ataggccgcg tcgacgaagg cccgaagggc tgtaccgtcg ccggcccggc cggaagctgg
     3181 agcgcgcggg gggactggac cgcgacccac catggctgag cgggttcggg taggggtgct
     3241 cgtctcgggg cgcggcacca acatggtcgc cctgagcgag cataagaggc gcgaggcgcg
     3301 aagctacgac gtcgctctgg tcgcctccaa cgtgccggag gcgcgcggcc tcgttgtcgc
     3361 ccgtcggctg ggaatcccgg tctgggcgcg aagccacaag ggcctggagc gggaagagtt
     3421 cgaccatttg ctcgacctcg cactgaggga gcatgacgtc gaaatggtgg cgctggccgg
     3481 ctacatgcgg ctgctctccg gcgaattcat ccgcaagtgg cccggccgga tcctcaacat
     3541 ccacccttcc ctgcttccgg cctataaggg cctggacacc caccgccggg cgctggaggc
     3601 gggcgagatc tgggtcggct gctcggtcca tctcgtcacc gaggagctgg acggggggcc
     3661 ggtgctggcc caggccaggg tccgggtgcg cgagcgggag acggcgcagc agctggcgga
     3721 gcgggtgctc agggaggagc accggctcta tccggagacc ctcgacaaat attgcgcccg
     3781 gctgaggccg gggcgggtgg aagttttgcc tccccaccgg tgaaaccggt ggggaggggg
     3841 accatgcgaa gcatggtgga ggggtgatct actgaggcct cgccaggcca cccctccacc
     3901 accttcggcg gtccccctcc ccatcctcga ggggga
//
LOCUS       DASWNU010000066         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_313228, whole genome shotgun sequence.
ACCESSION   DASWNU010000066 DASWNU010000000
VERSION     DASWNU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313228"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VGB57_01340"
     CDS             complement(<1..1416)
                     /locus_tag="VGB57_01340"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0130017.1"
                     /translation="MATAPMPETTQASSSFVNIGERTNVTGSARFKKLVMAGDYAAAV
                     EVARQQVENGAQVIDVNMDEGLLDAEYAMTTFLKLIAAEPDIARVPVMIDSSKWTVIE
                     AGLKCVSGKPIVNSISMKEGEEEFLNHARKVRDYGAAVVVMAFDEVGQADTKERKVEI
                     CERAYKLLVAEGFPPEDIIFDPNIFAIATGIEEHRTYALDFIAACREIKARCPHVHIS
                     GGLSNLSFSFRGNEPVRRAMHSIFLYHAIPAGLDMAIVNAGQLDVYDTIDPELREACE
                     DVIMDRRDDATERLVTLAGKFLGSDPVREKAEAEWRGWEVGKRLEHALVKGIDAHVVA
                     DTEEARQSAERPIHVIEGPLMDGMNVVGDLFGSGRMFLPQVVKSARVMKKAVAHLIPF
                     IEKEKELTGATQGKGRIVMATVKGDVHDIGKNIVGVVLQCNGFEIIDLGVMVPWQDIL
                     QAANEHNADIIGLSGLITPSLD"
     gene            complement(1532..2566)
                     /locus_tag="VGB57_01345"
     CDS             complement(1532..2566)
                     /locus_tag="VGB57_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY0130018.1"
                     /translation="MNVAQQLRAAAAERILIKDGAYGTQVQNRRLREADYAGSLGLSR
                     DQKGNNDLLNLTQPELVRDICQNFADAGAELLATNSFNANRISQADYGAENLVHEINC
                     AAARIIAEVADREAQKDGRKRYVVGALGPTNKTLSLSPNVNDPGYREIDFDTLSDVYV
                     EQIEGLVEGGAEIVLIETVFDTLNAKAAIHAAGRVDRELPIMLSMTITDLSGRNLSGH
                     SIEAFWASVRHARPLSIGLNCSFGAGQLRAHVAALSGIADTLIMAYPNAGLPNDLGEY
                     DEGPAETAAQVREWCEQGIVNIVGGCCGTTPAHIGAIAEAAKGFAPRRIAEPARRTLL
                     AGIEPMVLAA"
     gene            complement(2544..>2872)
                     /locus_tag="VGB57_01350"
     CDS             complement(2544..>2872)
                     /locus_tag="VGB57_01350"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY0130019.1"
                     /translation="DAAAAGIDAELVPGILPVSNVAQTRKFAAMCGAAIPAWMDKLFE
                     GLDDLPAARQLVAATVAAELCGQLYAGGVRQFHFYTLNRAELAYAICHLLGLRPKDEN
                     ERRTAA"
BASE COUNT          452 a          891 c          992 g          537 t
ORIGIN      
        1 gtcgagcgac ggagtgatca gcccggaaag cccgatgatg tctgcgttgt gctcgttcgc
       61 cgcctgcaag atgtcctgcc acggcaccat cacgccaaga tcgatgatct cgaagccatt
      121 gcactgcagg acgacgccga cgatgttctt cccgatgtcg tggacgtcgc ccttgacggt
      181 ggccatgacg atccggccct tgccctgggt ggcgcccgtc agctccttct ccttctcgat
      241 gaaggggatg aggtgggcga ccgccttctt catcacgcgg gccgacttga ccacctgcgg
      301 aaggaacatc ctgcccgatc cgaacaggtc gccgacgacg ttcatcccgt ccatcagcgg
      361 gccttcgatg acgtggatcg gccgctcggc ggattggcgc gcctcctccg tgtccgcgac
      421 gacatgggcg tcgatgccct tgacgagggc gtgctcgagc cgcttgccga cctcccagcc
      481 gcgccattcg gcttcggcct tttcacgcac gggatcggag ccgaggaatt tcccggcgag
      541 ggtgacgagc cgctcggtgg cgtcgtcccg gcggtccatg atcacgtctt cgcacgcctc
      601 gcgaagctcg ggatcgatcg tgtcgtagac gtcgagctgg ccggcgttga cgatcgccat
      661 gtcgaggccg gcgggaatcg cgtgatagag gaagatcgag tgcatggccc ggcgcacggg
      721 ctcgttgccg cggaaggaga agctcaaatt ggagaggccg cccgagatgt ggacgtgcgg
      781 gcacctggcc ttgatctcgc ggcaggcggc gatgaaatcc agggcgtagg tgcgatgctc
      841 ctcgatcccg gtggcgatgg cgaagatgtt gggatcgaag atgatgtcct cgggcgggaa
      901 gccctcggcg acgagcaatt tataggcgcg ctcgcagatc tcgaccttgc gctccttcgt
      961 gtcggcctgg ccgacctcgt cgaaggccat gacgaccacg gccgcgccat agtcgcgcac
     1021 cttgcgggcg tggttgagga actcttcctc cccctccttc atgctgatcg agttgacgat
     1081 cggcttgccc gacacgcatt tcagcccggc ctcgatcacc gtccacttgg agctgtcgat
     1141 catcaccggc acccgggcga tgtcgggctc ggccgcgatc agcttcaaaa aggtggtcat
     1201 cgcatattcg gcgtcgagca ggccttcgtc catgttgacg tcgatcacct gggcgccgtt
     1261 ctcgacctgc tggcgggcga cctcgaccgc ggcggcataa tcgccggcca tgaccagctt
     1321 cttgaagcgg gccgagccgg tgacgttggt gcgctcgccg atgttgacga aggaggagga
     1381 agcttgagtg gtctcgggca tgggtgcggt ggccattttt actcggttct ttggttttta
     1441 cgtcatgccg gacttgatcc ggcatccacc ttccctcgct gtcttcggaa gaaggtggac
     1501 cccggatcaa gtccggggtg acgacttggg gtcaggcagc gaggaccatc ggctcgattc
     1561 ccgcaagcaa ggtacggcgg gcgggctcgg cgatccgtcg gggcgcgaag cccttcgccg
     1621 cctccgcgat agcgccgata tgcgcgggag tggtgccgca gcagccgccg acgatgttga
     1681 cgatgccctg ctcgcaccat tcgcggacct gggcggcggt ttcggccggg ccttcgtcat
     1741 attcgccgag gtcgttgggc aggccggcat tgggataggc catgatcagg gtgtcggcga
     1801 ttcccgacag cgcggcgaca tgggcgcgga gctggcccgc gccgaacgag cagttgaggc
     1861 cgatagagag cggccgggcg tggcggacgc tggcccagaa ggcctcgatc gaatggccgg
     1921 aaaggttgcg gccggaaagg tcggttatgg tcatcgacag catgatcgga agctcgcggt
     1981 cgacgcggcc ggcggcgtgg atcgccgcct tggcgttcaa ggtatcgaac accgtctcga
     2041 tgaggacgat ctccgcgccg ccctcgacca gcccctcgat ctgctcgaca tagacgtccg
     2101 acaaggtgtc gaagtcgatc tcgcgatagc ccggatcgtt gacgttgggc gacagcgaca
     2161 gggtcttgtt ggtcggcccg agcgctccga cgacgtagcg cttgcgaccg tccttctggg
     2221 cctcgcggtc ggcgacctcg gcgatgatcc gggcggcggc gcaattgatc tcgtggacga
     2281 gattctccgc cccgtaatcg gcctggctga tgcggttggc gttgaagctg ttggtggcca
     2341 gcaattcggc tccggcgtcg gcgaaattct ggcagatgtc gcgcaccagc tccggctggg
     2401 tgaggttgag aaggtcgtta ttgcccttct ggtcgcgcga caggccgagg cttccggcat
     2461 aatcggcctc gcggaggcgc cgattctgca cctgggtccc gtaggcgccg tccttgatca
     2521 ggatgcgctc ggcggcggcg gccctaagct gctgtgcgac gttcattttc atccttcggg
     2581 cgaaggccga gcaaatggca gatggcgtag gccagctcgg cgcggttcaa ggtgtagaag
     2641 tggaactggc ggaccccgcc ggcataaagc tggccgcaaa gctcggccgc tacggtggcg
     2701 gcgacgagct ggcgcgccgc cggcaggtcg tcgaggccct cgaagagctt gtccatccag
     2761 gccgggatgg cggcgccgca catggccgcg aacttgcggg tctgggcgac gttggagacg
     2821 ggcaggatcc ccggcaccag ctcggcgtcg atccccgccg ccgccgcgtc gt
//
LOCUS       DASWNU010000067         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_27984, whole genome shotgun sequence.
ACCESSION   DASWNU010000067 DASWNU010000000
VERSION     DASWNU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27984"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VGB57_01355"
     CDS             <1..133
                     /locus_tag="VGB57_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0130020.1"
                     /translation="WIARLQPKRALLTHLDNSMDYDTLVDELPDGVEPAYDGMEIEL"
     gene            130..726
                     /locus_tag="VGB57_01360"
     CDS             130..726
                     /locus_tag="VGB57_01360"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY0130021.1"
                     /translation="MNDGDQAATFLYLIGVLVLVVSALMVRRIPIGQGLKMFAGWVLI
                     FLAFFVAFALKDDFMALGKRVVAESRGEGQVVAAGRELRIRKSMDGHFWVDAELNGKK
                     VRFLVDSGATVTSISSDTAAMADIVPSGLPAVVQTANGTVRVGRARAERLTVGSIERR
                     DLAVHVSEAFGPMNVLGMNFLSSLSAWGVEGQWLVLKP"
     gene            814..1566
                     /gene="mazG"
                     /locus_tag="VGB57_01365"
     CDS             814..1566
                     /gene="mazG"
                     /locus_tag="VGB57_01365"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY0130022.1"
                     /translation="MSDSSTSLSDLVEIMRRLRDPGRGCEWDKAQSFETIAPYTIEEA
                     YEVADAIAREDLEGLKDELGDLQLQVVYHARMAEEIGAFALDDVMASICAKMIRRHPH
                     IFGDEAASPGWETLKAAERHGGEDKSALAGVALALPALKRAEKLQRRAARIGFDWPDG
                     SGPRVKIDEELGELDDAATAEERAAELGDLLFSVVNYARHLEIDPEAALRESSARFEQ
                     RFRKVEEIADKPLKDMDIEALEALWQRAKKLV"
     gene            complement(1652..2881)
                     /gene="hflX"
                     /locus_tag="VGB57_01370"
     CDS             complement(1652..2881)
                     /gene="hflX"
                     /locus_tag="VGB57_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0130023.1"
                     /translation="MKAGRGRRDIDARLDEAAGLAEAIGVEVSDRLHFRIRSPRPATL
                     FGSGQVESIAEAVARDEAQLVIVDAPITPIQQKNLETATKAKVIDRTGLILEIFGERA
                     ATSEGRLQVELAHLDYQAGRLVRSWTHLERQRGGFGFLGGPGETQIEADRRLIRDRMA
                     KLKKELEQVTRTRALHRARRQRAPWPVIALVGYTNAGKSTLFNRLTNADVMAENLLFA
                     TLDPTMREISLPGFDKAILSDTVGFISELPTQLVAAFRATLEEVVSADLIIHVRDISH
                     PDSDAQREDVEAVLAEIGAGGEGGAPLVEAWNKIDLLDGEESERVQAEAVRRDDVITI
                     SALTGEGLDDLLECASVKLSKGAKLRTVTLGASDGETIAWLHANGRIESQQTDGVETV
                     FEVRLSEADWARLQSRL"
     gene            complement(2950..3456)
                     /gene="hfq"
                     /locus_tag="VGB57_01375"
     CDS             complement(2950..3456)
                     /gene="hfq"
                     /locus_tag="VGB57_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY0130024.1"
                     /translation="MADKVNNLQDLFLNGVRRSKTPVTMFLVKGVKLQGIITWFDNFS
                     VLLRRDGQAQLIYKHAISTIMPAHPLDISEIERAFEEGGSGGGGKKPALLQEAFLRAV
                     RRSGEPVTMFLVNGVMLQGEIAAFDLFCMLLQRDGLSQLVYKHAISTVQPERPVSFME
                     QDDEEPAA"
     gene            complement(3666..>4386)
                     /locus_tag="VGB57_01380"
     CDS             complement(3666..>4386)
                     /locus_tag="VGB57_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0130025.1"
                     /translation="GLLEQAHGGTLFLDEIADMPIPAQAKILRVLTDQSFTRVGGQRM
                     VKVDMRVVSSTSRNLADEIAGGRFREDLFYRLNVVPVHLPALAERREDIPALIAHFAA
                     RYAAEQRLPTPEFSNDAVAALQAYDWPGNVRQLRNVIERTIILAPGARVGCIELDMLP
                     PEILNEQASLSSGEAVKAIMGTPLREARETFEREYLKVQIRRFSGNISRTATFIGMER
                     SALHRKLKALGIGDSKGEPEE"
BASE COUNT          716 a         1409 c         1494 g          767 t
ORIGIN      
        1 ctggatcgcc cggcttcagc cgaagcgggc gctgctcacc cacctcgaca acagcatgga
       61 ctacgacacc ctggtcgacg agcttccgga cggagtcgag ccggcttatg acggcatgga
      121 aatcgagctg tgaacgacgg cgaccaggcg gccaccttcc tttatctgat cggagtcctc
      181 gtcctcgtcg tcagcgcgct catggtccgc cggataccga ttggccaagg gctcaagatg
      241 ttcgccggct gggtgctcat cttcctcgcc ttcttcgtcg ccttcgcgct caaggacgat
      301 ttcatggccc tcggcaagcg ggtcgtcgcc gagagccggg gggaggggca ggtcgtcgcc
      361 gccggcaggg agcttcggat ccgaaagtcg atggacggcc acttctgggt cgatgccgaa
      421 ctcaacggca agaaggtgcg cttcctggtc gacagcggag cgacggtcac cagcatctcg
      481 agcgacaccg cagccatggc cgacatcgtg ccgagcggtc tgccggccgt cgtccagacg
      541 gcgaacggca ccgtccgggt cggacgcgcg cgcgccgagc ggctcaccgt cggatcgatc
      601 gagcggcgcg acctggccgt ccatgtctcc gaagcgttcg ggccgatgaa cgtgctcggg
      661 atgaacttcc tgtcttcgct ttcggcctgg ggcgtcgaag gccagtggct ggtgctcaag
      721 ccgtgagggg ggatccgcct cccttgaacc tggctctgta ttttacataa tgtatattat
      781 cgcacttaaa cattgacggg aagagccgcg cctgtgtccg attcgtccac ttccctctcc
      841 gacctggtcg agatcatgcg ccggctgcgt gaccccggcc gcggctgcga atgggacaag
      901 gcgcagagct tcgagacgat cgcgccatac acgatcgagg aagcctatga ggtcgcggac
      961 gcgatcgccc gcgaggatct cgagggtctc aaggacgagc ttggcgacct ccagctccag
     1021 gtcgtctatc acgcccggat ggccgaggag atcggcgcct tcgcgctgga tgacgtcatg
     1081 gcgtcgatct gcgccaagat gatccgccgc cacccgcaca tcttcggcga cgaagccgcc
     1141 agcccgggct gggagaccct gaaggcagcc gagcgccacg gcggcgagga caagagcgcg
     1201 ctggccggag tcgcactcgc attgcccgcc ctgaagcgcg ccgagaagct tcagcgccgg
     1261 gccgcccgaa tcggcttcga ctggcccgac gggtcgggac cgagggtcaa gatcgacgag
     1321 gagctgggcg agctcgacga tgccgccacc gccgaagagc gcgccgccga gctcggcgac
     1381 cttctgttca gcgtcgtcaa ttacgcccgc cacctcgaaa tcgatccgga agcggctctg
     1441 cgcgaatcct cggcccgctt cgagcaacgc ttccgcaagg tggaagagat agccgacaag
     1501 cctttgaagg atatggatat cgaggcgctg gaggcgcttt ggcaaagggc gaagaagctg
     1561 gtctgaccgt catcccggcg aaagccggga cctcgggccg ttaaagtctc ggcgggtctc
     1621 cccgaggtcc cggctttcgc cgggatgacg gtcaaagccg gctctgaagc ctcgcccagt
     1681 ccgcctcgga gagccgcacc tcgaacaccg tctcgacgcc gtccgtctgc tggctctcga
     1741 tccggccgtt ggcgtggagc caggcgatcg tctcgccgtc cgacgctccg agcgtgacgg
     1801 tgcgaagctt ggcgcccttg ctgagcttga ccgacgcaca ttcgaggaga tcgtccaagc
     1861 cctcgccggt caaggcggag atggtgatca cgtcgtcgcg gcgcaccgcc tccgcctgca
     1921 cccgctcgct ttcttcgccg tccagcaggt cgatcttgtt ccacgcctcg acgagcgggg
     1981 cgcccccctc ccctccggcg ccgatctcgg cgaggacggc ttcgacgtcc tcgcgctggg
     2041 cgtcgctgtc gggatgcgag atgtcgcgga cgtggatgat gagatcggcc gagaccactt
     2101 cctcgagcgt ggcccgaaaa gcggcgacga gctgggtcgg aagctccgag atgaagccga
     2161 cggtgtcgga gaggatcgcc ttgtcgaagc cgggcaggga gatttcgcgc atcgtcgggt
     2221 cgagggtcgc gaagagcagg ttctcggcca tcacgtcggc gttggtgagc cggttgaaca
     2281 gggtcgactt gccggcattg gtgtagccga ccagagcaat caccggccag ggcgcgcgct
     2341 ggcggcgggc acggtggagg gcccgggttc gggtgacctg ctcgagctcc ttcttgagct
     2401 tggccatccg gtcgcggatc agccgccggt cggcctcgat ctgggtctcg ccggggccgc
     2461 cgaggaagcc gaagccgccg cgctggcgct cgaggtgggt ccagctcctg accaggcggc
     2521 ccgcctgata gtcgagatgg gcgagctcga cctggagccg cccttccgac gtcgccgccc
     2581 gctcgccgaa aatctccagg atgagaccgg tccggtcgat caccttggcc ttggtcgcgg
     2641 tctcgagatt cttctgctgg atgggcgtga tcggggcgtc gacgatgacc agctgggctt
     2701 cgtcgcgggc gaccgcctcg gcgatcgatt cgacctgccc cgagccgaac agggtcgccg
     2761 gccggggcga gcgaattcgg aagtggagcc tgtcggagac ttcgaccccg atcgcttcgg
     2821 cgagccccgc ggcctcgtcg aggcgagcgt cgatgtcgcg ccgtccccta cccgccttca
     2881 tgtcgggaag agcgacgatc gccctggcgc cgcggctgaa ctcgccgtcg gaactttgtt
     2941 cggtggaagt caggcggccg gctcctcgtc atcctgctcc atgaagctca ccggccgttc
     3001 cggctgcacg gtcgagatgg cgtgcttgta gacgagctgc gacaggccgt cgcgttggag
     3061 aagcatgcag aacagatcga aggccgcgat ttctccctga agcatgactc cgttgaccaa
     3121 aaacatggtg accggctcgc ccgagcgacg gaccgcccgg aggaatgctt cctggagcaa
     3181 cgccggcttc ttgccgccgc cgccgctgcc gccttcctcg aaggcacgct cgatctcgct
     3241 gatgtcgagc ggatgcgcgg gcatgatcgt cgagatggcg tgcttgtaga tgagctgggc
     3301 ctggccgtcg cggcgaagca gcaccgagaa attgtcgaac caggtgatga tcccctggag
     3361 cttcacgccc ttgacgagga acatggtcac gggcgtcttc gaacggcgaa cgccgttcag
     3421 gaacagatcc tggaggttgt tgaccttgtc ggccatgtcc cgctctcctt attggcggat
     3481 cgttgtccgc ttcaggcgcc cgcgaagggc gtctttcata atgtggagcg cagcgtgccg
     3541 gaaacaatag catccgacag ggcacggcgc cagtttggtc ctccccatgc gcagcatggg
     3601 gagggggacc acgtgaaacg tggtggaggg gtagctggct cggaattcga ggttctgagg
     3661 attagctact cctccggctc gcctttcgaa tctccgatgc ccagcgcctt cagcttccgg
     3721 tgcaacgccg agcgttccat tccgatgaag gtggcggtgc gggagatgtt tccggagaag
     3781 cggcggatct ggaccttcag atattcgcgc tcgaacgttt cccgggcttc ccgaagcggg
     3841 gtccccatga tcgccttgac cgcctcgccc gagctgaggc tggcctgctc gttgaggatt
     3901 tcgggcggga gcatgtccag ttcgatgcag ccgacccggg cgccgggcgc caggatgatg
     3961 gtgcgctcga tgacgttcct cagctggcgg acgttgcccg gccagtcgta ggcctggagc
     4021 gccgcgaccg cgtcgttcga gaattccggc gtcggaagcc tctgctcggc cgcgtagcgc
     4081 gccgcgaaat gggcgatcag agcgggaatg tcctcgcgcc gctcggccag cgccggcagg
     4141 tgaacgggca cgacgttcag ccggtagaag aggtcttcgc ggaagcgtcc gccggcgatc
     4201 tcgtcggcga ggttgcgcga ggtggaggag acgacccgca tatcgacctt gaccatgcgc
     4261 tgccccccga cccgggtgaa gctctggtcg gtcaggaccc gcaagatctt ggcctgggcc
     4321 gggatcggca tgtcggcgat ctcgtccagg aagagggttc cgccatgggc ctgctcgagc
     4381 aggccg
//
LOCUS       DASWNU010000068         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_80168, whole genome shotgun sequence.
ACCESSION   DASWNU010000068 DASWNU010000000
VERSION     DASWNU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80168"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VGB57_01385"
     CDS             complement(<1..1026)
                     /locus_tag="VGB57_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0130026.1"
                     /translation="MHGNFQTSGFSDALVILGAAGVVIPAFARFRISPVIGFILVGLL
                     VGPAGLGAMAGRMPWLNYVTISNPEAVEPFAEFGIVLLLFAIGLELSFRRLWTMRRLV
                     FGVGAAELLVCALLIGFALHMLGQSISGSVALGLALALSSTALVLPLVGTTSPVGRSA
                     LAMLLFEDLAIVPIIFLLGAMAPFAAASGFDGLVNTLLLGAAVVAAMLVLGRLLLPPL
                     FAQAARTKSPELFLAASLLVVIVASLATTAAGLSPLMGALLAGILIAETDYRSEVEVI
                     TAPFRGLALGIFLITVGMSVDVRIVAANWEYIALAVIGIVLLKAVVTGLLLRLNGARR
                     AVAAEAGL"
     gene            complement(1258..>2358)
                     /locus_tag="VGB57_01390"
     CDS             complement(1258..>2358)
                     /locus_tag="VGB57_01390"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0130027.1"
                     /translation="HYYDLSVEERDRTEDQVTVDSAHAIQKYGVGVKCATITPDEARV
                     EEFGLKKMWKSPNGTIRNILGGVVFREPIVIETVPRLIPGWTDPIVVGRHAFGDQYKA
                     TDFKVPGPGKLTIKWQGEDGQVIEHEVFDFPSSGVAMGMYNLDDSIRDFARACLNFGL
                     GRGWPVYLSTKNTILKAYDGRFKDIFQEIFDSEFKEKFDAAGTEYQHRLIDDLVASAL
                     KWSGKFVWACKNYDGDVQSDMVAQGFGSLGLMTSILMTPDGKIVEAEAAHGTVTRHYR
                     MHQQGKATSTNPLASIFAWTGGLKFRGKFDDTPEVTRFAETLERVCIETVEAGQMTKD
                     LAILIGPEQSWMTTEQFFEAIRANLEKAMAEG"
BASE COUNT          425 a          760 c          797 g          376 t
ORIGIN      
        1 cagccccgct tcggcggcca ccgcccggcg ggcgccgttt agcctcagca gcaggcccgt
       61 aaccaccgcc ttgagcagca cgattccgat caccgcgagc gcgatatatt cccaattggc
      121 ggcgacgatg cgcacgtcca cgctcatccc gaccgtgatc aggaagatgc cgagtgcgag
      181 cccgcggaac ggagcggtga tgacctccac ctcgcttcga tagtcggtct ccgcgatcag
      241 gatcccggcc agcaacgcgc ccatcagcgg cgacaggccc gccgcggtgg tggcgaggct
      301 ggcgacgatc accaccagca ggctcgcggc gaggaacagc tcggggctct tggtccgggc
      361 cgcctgggcg aacaggggcg gaagcaggag ccgcccgagc accagcatcg cggcgacgac
      421 ggcggcgccc agcagcaggg tgttgacgag gccgtcgaag ccgctcgccg cggcgaaggg
      481 cgccatcgcc cccaggagga agatgatcgg gacgatcgcc agatcctcga acaacagcat
      541 cgccagcgcc gagcggccga ccgggctggt ggtgccgacc agcggcagga cgagggcggt
      601 cgaggagagt gccagcgcaa ggcccagtgc gaccgagccg ctgatgctct ggccgagcat
      661 gtgcagcgca aaaccgatga gcagcgcgca gaccagaagc tccgcggcgc caaccccgaa
      721 caccagtcgc cgcatcgtcc acagtcgtcg gaacgacagc tcgagcccga tggcgaagag
      781 caggaggacg ataccgaact cggcgaaggg ttcgaccgcc tcgggattgg agatggtgac
      841 gtagttcagc cacggcatcc gtcccgccat cgcgcccagc ccggccgggc cgacgagaag
      901 cccgaccagg atgaagccga tgacggggct gatccggaac cgggcgaagg ccgggatgac
      961 caccccggcg gcgccgagga tgaccagggc gtcgctgaag cccgaagtct ggaaattgcc
     1021 gtgcatccct gcctttatgc aggaacggga ggcgatgtca gcaataggcg gagaacgagc
     1081 cgccattttg cctccccgtc ggcggagccg atggggaggg gcaccacccg aagggtggtg
     1141 gaggggtaac tgccgtagat taggaggatc gcgctcgaac cggttgatcc ggcgccattt
     1201 acccctccac catgcttcgc atggtccccc tccccatgct tcgcatgggg aggcagatta
     1261 cccttccgcc atcgcctttt cgagattggc gcggatcgcc tcgaagaact gctccgtggt
     1321 catccagctc tgctcggggc cgatcaggat ggcgaggtcc ttggtcatct ggccggcctc
     1381 gacggtctcg atgcagaccc gctcgagggt ctcggcgaag cgggtgacct cgggcgtgtc
     1441 gtcgaacttg ccgcggaact tgaggccccc ggtccaggcg aagatcgagg cgagcgggtt
     1501 ggtcgaggtc gccttgccct gctggtgcat ccggtaatgg cgggtgacgg tgccgtgggc
     1561 ggcttccgcc tcgacgatct tgccgtccgg ggtcatcagg atagaggtca tcagtccaag
     1621 cgagccgaag ccctgggcga ccatgtcgga ctggacgtct ccgtcgtaat tcttgcaggc
     1681 ccagacgaac ttgccgctcc acttcagagc cgaggcgacg aggtcgtcga ttaggcgatg
     1741 ctgatattcc gtgcccgccg cgtcgaactt ctccttgaac tcgctgtcga aaatctcctg
     1801 gaagatgtcc ttgaagcggc cgtcataggc tttgaggatg gtgttcttgg tcgacagata
     1861 gaccggccag ccgcggccca ggccgaaatt gaggcaggcg cgggcgaagt cgcggataga
     1921 atcgtcgaga ttgtacatgc ccatggcgac tcccgacgag gggaagtcga acacctcatg
     1981 ctcgatcacc tggccgtcct cgccctgcca cttgatggtg agcttgccgg ggccggggac
     2041 cttgaagtcg gtcgccttat actggtcgcc gaaggcgtgg cggccgacga cgatcgggtc
     2101 ggtccagccc gggatcagcc ggggcacggt ctcgatcacg atcggctcgc gaaagacgac
     2161 tccgcccagg atgttgcgga tcgtcccgtt cggcgacttc cacatcttct tgaggccgaa
     2221 ctcctcgacc cgcgcctcgt cgggcgtgat cgtcgcgcat ttcacgccga cgccgtactt
     2281 ctggatggcg tgggccgagt ccacggtgac ctgatcctcg gtgcggtccc gctcctcgac
     2341 actgagatcg tagtagtg
//
LOCUS       DASWNU010000069         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_28090, whole genome shotgun sequence.
ACCESSION   DASWNU010000069 DASWNU010000000
VERSION     DASWNU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28090"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VGB57_01395"
     CDS             complement(<1..1495)
                     /locus_tag="VGB57_01395"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEY0130028.1"
                     /translation="MTRLFAALLLLAGLTAPAFAQSRAEIERTMKRATTFMVEKASTK
                     GGYVWSYLPDMSRRWGEIEAKPSMIWVQPPGTATMGHLFLDAWHATGDEYYYQAAEKV
                     GGALIWGQHRSGGWNYFIDFAGPSSTKHWYETIGRNAWRMEEFQHYADNATFDDMGTS
                     ESAQFLLRLYLEKRDPKYRPALERAVNFVLESQYPSGGWPQRWPYDPNYPAYQSYITF
                     NDDVAAENVKFLTMVYQHLGDTRALDAIHRGMNIYLLTQQGAPQAGWALQYTLDLKPA
                     AARTYEPNALHTGTSASNVEQLMNFYRMTGDRRFLARIPEALAWLDSVRLPEPRQGRS
                     FPTFVEIGSNRPIYVHRRGSNVVNGEYYWDYSPEATLGHYSAFRNVDVAKLRREYEAL
                     LNTSPADLAKSSPLLAPDPEPLPRYFIGGGMVGSDLNAGAVTATPRELIRSLNAQGWW
                     PTPLRATSNPYRGAGPAEPVPGDYRTTHVGDPSDTSPFTTDTPVTGIS"
     gene            complement(1495..>1846)
                     /locus_tag="VGB57_01400"
     CDS             complement(1495..>1846)
                     /locus_tag="VGB57_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0130029.1"
                     /translation="IYPVVSMTLPVAHAGSRRNLIGENASPERERAHSPHLNVPANAP
                     PTFLLHAEDDSSVVVENALLLRSALLARKVPVETHLFADGGHGFGLRLARGKTVEGWQ
                     DLFFAWARKKGLFE"
BASE COUNT          292 a          551 c          682 g          321 t
ORIGIN      
        1 tggagatgcc cgtgaccggc gtgtcggtcg tgaaaggcga ggtgtcgctg gggtcgccga
       61 catgggtggt ccggtagtcg cccgggaccg gctcggcggg gccggcgccg cggtacgggt
      121 tgctggtcgc ccgaagcggg gtcggccacc agccctgggc gttcagcgat cggatcagct
      181 cgcgcggggt cgccgtgacg gcgccggcgt tgaggtcgga gccgaccatt ccgccgccga
      241 tgaaatagcg gggcaggggc tccggatcgg gcgcgagcag tggcgagctc ttcgccagat
      301 cggccgggga ggtgttgagc agcgcctcat attcgcgcct cagcttcgcc acgtcgacgt
      361 tgcggaaggc ggaataatgg ccgagcgtcg cttcggggct gtagtcccaa taatattcgc
      421 cgttgacgac gttcgagccg cggcggtgga cgtagatcgg ccggttgctt ccgatctcga
      481 cgaaggtcgg gaagctgcgg ccctggcgcg gctcgggcag gcgcaccgag tccagccagg
      541 ccagggcttc ggggatccgg gcgaggaagc ggcggtcgcc ggtcatccgg tagaaattca
      601 tcagctgctc gacgttcgac gcggaagtgc cggtgtggag cgcgttcggc tcgtaggtcc
      661 gagcggcggc gggcttgagg tccaatgtat attgcagcgc ccagccggcc tggggggccc
      721 cctgctgggt cagaaggtag atgttcattc cgcggtggat ggcgtcgagg gcgcgggtgt
      781 cgccgagatg ctggtagacc atggtcagga acttgacgtt ctcggcggcg acgtcgtcgt
      841 tgaaggtgat gtagctctga taggcgggat aattcgggtc gtagggccag cgctgcggcc
      901 agccgccgct cggatactgg ctctcgagca cgaaattgac cgcgcgctcc agcgcgggcc
      961 ggtatttcgg atcgcgcttc tcgagataga ggcggagcag gaactgggcg ctttccgaag
     1021 tgcccatgtc gtcgaacgtg gcgttgtcgg cataatgctg gaattcctcc atccgccacg
     1081 cattcctgcc gatcgtctcg taccaatgct tggtcgacga ggggcccgcg aaatcgatga
     1141 agtaattcca gccgccgctt cggtgctggc cccagatgag ggcgccgccg accttctcgg
     1201 cggcctgata ataatattcg tcgcccgtgg cgtgccaggc gtccaggaag aggtgaccca
     1261 tggtcgcggt gcccgggggc tggacccaga tcatgctcgg cttggcctcg atctcgcccc
     1321 agcgacgcga catgtcgggg aggtaggacc agacgtagcc gcccttggtc gacgccttct
     1381 cgaccatgaa ggtcgtggcg cgcttcatgg tccgctcgat ctcggcgcgg ctctgggcga
     1441 aagccggggc ggtgagcccg gccaggagga gcagggcggc gaacagtctc gtcatcactc
     1501 gaacaatccc ttcttgcggg cccaggcgaa gaacagatcc tgccagccct ctaccgtttt
     1561 gccacgggcg aggcgaaggc cgaagccgtg gccgccgtcg gcgaacagat gcgtttcgac
     1621 cggtaccttc ctcgccagca gcgccgaccg gagcagcaaa gcgttctcga ccacgacgga
     1681 cgaatcgtcc tccgcgtgga ggaggaaggt gggcggagcg ttcgccggca cgttcaggtg
     1741 cggcgaatgg gcgcgctcgc gctcggggga ggcgttctcg ccgatcaggt tccggcgcga
     1801 tccggcatgg gcgaccggca gggtcatcga cacgacggga tagatc
//
LOCUS       DASWNU010000070         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_470390, whole genome shotgun sequence.
ACCESSION   DASWNU010000070 DASWNU010000000
VERSION     DASWNU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470390"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..756)
                     /locus_tag="VGB57_01405"
     CDS             complement(280..756)
                     /locus_tag="VGB57_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130030.1"
                     /translation="MLKKILTAAAAAAAALTVLPASASAGHGRGGHDGGYYDGYGRGY
                     EGRGYEDRGYEERGYYGRGGYDRGYGDRGYYGRGGYDRGYYARGYGYRARGHYGCGSG
                     TTGAIVGGITGAAIGRDIDSGGRRSRWRGRDDGGTGAIIGGAVGALLGREIARSGC"
     gene            906..1151
                     /locus_tag="VGB57_01410"
     CDS             906..1151
                     /locus_tag="VGB57_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130031.1"
                     /translation="MTENEDKIGGRGGGGDSGGGAYPNPHSGKDGDRGGFMDHGGQTE
                     MPYHGSGRLGDEKTGDNPNVPTGKAKPGDKGTSGSRE"
     gene            1398..>4137
                     /locus_tag="VGB57_01415"
     CDS             1398..>4137
                     /locus_tag="VGB57_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130032.1"
                     /translation="MAGNILLVLDGDFNFDPPAPTPKFTYSVLVQTLEAAGFTVTRAH
                     RGTDDSANTQNGFRFDAPTDLSGFDLLWLIGDLGFNQGADASAPLDPSEVLAIAQFME
                     AGGGVFATGDHDSIGSKMCGDIPRVRAMRKWFAVADPLRPAGFPGNWPPTGADRADTI
                     QPNAHGDGGTYFENQSDSTPQPIQRVPPEGPTHPILINGDHDVQVFPDHMHEGDCIAG
                     WDGFDWGGNPIDGSPIVEFRTLTGDPIERPQVIATGEGLGHATPAGAGAFGEDTPSIP
                     KTIGVLAAYDGFAVGVGRIVTGSTFHHYVDLNLTGDARVDSQDEFNTVGEDAQDEEGF
                     DWPAASQVFADIKAVYVNIANWLSRPRRAIRLILDRSTFGQDEVVASLDDAEPGLFAR
                     SFFVAVEGFRPSDFPGPDGLDTLSPSQADLEAWAPDIPSPLGTNIAVRVTGVSSSGPQ
                     LLERFQTFTFEYSVRFPNGEAFGFGDPTEDFLVNASLNLGGTAMSDSAVIRLVKTANP
                     FMVDLADGNDTVWLSSDLRLFTLTEGSSRFGKTLPDNATPAQAAAFIADVIDELDPAD
                     FADLPLGQAESPLSPYPTADNGKTIYNFAIARVRLRGEALDASDVRVFFRIFESQTTA
                     ALTFEGPFGSYMRTDPPDPIALPGESGSQYISFPCFATARDSDPADQEDLPNVQPIAV
                     TPGGTSFAFFGCLIDNNVEIPYLPPAPGSMQPEISLSALLTGAHQCIVAQIEHEGTAI
                     PVGANPATSDKLAQRNIALDHIANPGRDASRVATHTFEIAATPDPIREDWRPDELMLE
                     WRGRVPEGARVSFYLPTCRASAVIALADKLYTRHGLRALDDNTIEVAAGGTSYIPLPQ
                     SLAPRVGLMSAAFPLGVRKGERYDCLVRQVTNRMRRVQQPPAKVTRLDREEADRKMA"
BASE COUNT          722 a         1470 c         1287 g          658 t
ORIGIN      
        1 cgccgcgccg gtcgtaacgg tcgtaaaatt gcgccgaagc aggcgctgcc gatccgagcg
       61 cgaccgcggc ggccgcggtg ccgagcagga acttcttcat ggcatctctc caaacaatcg
      121 cccgaagcgc cgggctatgg atgagggtct aggctagtgg ccatgacctc gccctgaacc
      181 ccctgtcgcg ggaggttcat gttccaaaac agaggcttga tggagaaagg cggagccgct
      241 cgcgccgccc cgccttcccg gattcagtga tggggcgcgt cagcagccgc tgcgcgcgat
      301 ctcgcgtccg agcaaagccc cgacggcgcc gccgatgatc gcgccggtgc cgccgtcgtc
      361 gcggccccgc cagcgcgacc gcctgccgcc gctgtcgatg tcgcggccga tcgcggcgcc
      421 ggtgatgccg ccgacgatcg cgccggtggt cccgctgccg cagccataat ggccgcgcgc
      481 ccggtagcca tagccccggg cgtaatagcc gcggtcgtag ccgccgcgtc cgtaatagcc
      541 ccggtcgccg taaccgcggt catagccgcc gcgcccgtaa tatccgcgct cctcatagcc
      601 ccggtcttca tagccccgac cctcgtaacc gcgcccgtag ccgtcataat agccgccgtc
      661 atggccgccg cggccgtggc cggccgaagc ggaagcggga aggacggtga gggccgccgc
      721 tgcagcggcc gccgcggtga ggatcttctt gagcatgtta caggctcccg ggaaaacaga
      781 ggaatggcgt cgatcctatc tcgctccctt ttgctgagcg gaggatgaac ggcccttctt
      841 cgaccttctg gaacaggagg gccgctccgc gcgtcgcacc ggcaaagctg atacgaggtg
      901 cagacatgac ggagaatgaa gacaaaatcg gcggtcgcgg cggcggcggc gattcggggg
      961 gcggcgccta tcccaatccg cacagcggca aggacggcga tcgcggcggt ttcatggacc
     1021 atggcggcca gaccgaaatg ccctatcacg gctccggtcg cctcggcgat gaaaagaccg
     1081 gcgacaatcc caatgtgccg accggcaagg caaagccggg tgacaagggg acatcgggct
     1141 cccgcgaata gtcaatcgga gcagtcgacg gcaacattat tgggacggga cgagtcgcag
     1201 cggcgaagcc ttgatttatc gtccgatctg gagctcaaaa acgaagccgc gtgccgcttg
     1261 aacctcaatc atctataaat aaacgagatt catcccgatt gactcgagtg gctctcgggc
     1321 acgatcatga ctgacgattc cacgatcgtg ggagtcgtcc gtacctccat tgcgggcggc
     1381 gcagggggag ggcagtcatg gccggaaaca ttcttctcgt tctcgacggc gacttcaact
     1441 tcgatccgcc ggcgcccact cccaaattta cctattccgt tctcgtccag actctcgagg
     1501 ccgcgggctt cacggtcacc agggcacatc gcggtaccga cgattcggcg aacacgcaga
     1561 atggtttccg cttcgacgcg ccgaccgacc tgtcgggctt cgacctgctg tggctcatcg
     1621 gcgatctcgg attcaaccag ggtgcggacg ccagcgcgcc gctcgatccc agcgaggtgc
     1681 tggcgatcgc ccagttcatg gaagcggggg gcggggtctt cgctaccggc gaccatgaca
     1741 gcatcggctc gaagatgtgc ggcgacatcc cgcgcgttcg ggcgatgcgc aaatggttcg
     1801 cggtcgccga tccgctgcgt cctgccggct ttccgggcaa ttggccgccg acgggagccg
     1861 accgcgccga cacgatccag cccaacgccc atggcgacgg cgggacctat ttcgagaacc
     1921 agtccgattc gaccccgcag ccgatccagc gggttccacc cgaaggtccc actcacccga
     1981 tcctcatcaa cggcgatcac gacgtccagg tcttccccga tcacatgcac gaaggcgatt
     2041 gcatagcggg ttgggacggc ttcgattggg gtggcaaccc gatcgacggc tcgccgatcg
     2101 tcgagttccg cacgctcacc ggcgatccga tcgagcggcc gcaggtgatc gccaccgggg
     2161 aagggctcgg ccacgccact ccggccggcg ccggcgcgtt cggggaagac acgccctcca
     2221 tccccaagac gatcggcgtg ctcgcggcct atgacggctt cgccgtaggc gtcggccgca
     2281 tcgtcaccgg ctcgaccttc caccattatg tcgacctcaa cctgaccggc gacgcccgcg
     2341 tcgacagcca ggacgagttc aacacggtcg gcgaggacgc gcaggacgag gagggtttcg
     2401 attggcccgc cgcctcccag gtcttcgccg acatcaaggc cgtctatgtc aacatcgcca
     2461 attggctgtc gcgcccgcgg cgcgcgatcc ggctcattct cgatcgcagc accttcggcc
     2521 aggacgaagt cgtcgcctcg ctggacgatg ccgagccggg cctgttcgca cgctccttct
     2581 tcgttgcggt cgagggcttc cggccgagcg atttcccggg gccggacggc ctcgataccc
     2641 tcagtccgag ccaggccgat ctcgaggcct gggctccgga catccccagc cccctgggaa
     2701 ccaacatcgc ggtccgggtg accggggtaa gctcgagcgg cccgcagctg ctcgagcgct
     2761 tccagacctt cactttcgaa tatagcgtcc gcttccccaa cggggaggca ttcggcttcg
     2821 gcgatccgac cgaggatttc ctcgtcaatg catcgctcaa cctcggcggg acggcgatga
     2881 gcgacagcgc cgtcatccgg ctcgtcaaga ccgcgaaccc gttcatggtc gatctcgccg
     2941 acgggaacga cacggtctgg ctgagctctg acctgcggct gttcacgctc accgaaggct
     3001 cgagccggtt cggaaagacc cttcccgaca acgcgacgcc cgcgcaggcc gccgccttca
     3061 tcgccgacgt catcgacgag ctggaccccg cggactttgc cgacctgccg ctcggccagg
     3121 cggaatcgcc gctcagcccc tatccaaccg cggacaacgg caagacgatc tacaatttcg
     3181 cgatcgcgcg cgtgcgcctg cgcggcgagg ccctagacgc cagcgacgtc cgcgtctttt
     3241 tccgaatctt cgaatcgcag acgaccgcag cgctcacctt cgagggcccc ttcggcagct
     3301 atatgcgcac cgatccgccg gacccgatcg ccctgcccgg ggagagcggc agccaatata
     3361 tctccttccc ctgcttcgcg accgcgcggg atagcgatcc cgcggaccag gaagatctgc
     3421 ccaacgtgca gcccatcgcc gtgacccccg gcggcaccag tttcgctttc ttcggctgcc
     3481 tcatcgacaa caatgtcgaa attccatatt tgccgcccgc gcccggctcc atgcagcccg
     3541 agatcagcct ctccgctctg ctgaccggcg cgcaccaatg catcgtcgcg cagatcgagc
     3601 atgaggggac ggccatcccg gtgggggcca atcccgcgac cagcgacaag ctcgcccagc
     3661 gcaacatcgc cctcgatcac atcgccaatc ccggccgcga cgcgtcccgt gtcgccaccc
     3721 ataccttcga gatcgcggcg acgcccgacc cgatccgcga ggactggcgg cccgacgagc
     3781 tcatgctcga atggcgcgga cgggttccgg agggcgcgcg cgtcagcttc tatctcccca
     3841 cctgccgcgc ctcggcggtg atcgcgctcg ccgacaagct ctacacacgc cacggattgc
     3901 gcgcgctcga cgacaacacg atcgaagtgg cggcgggcgg tacaagctac atcccgctgc
     3961 cgcagagcct cgcgccccgc gtcggcctca tgtcggccgc cttcccgctc ggagtccgca
     4021 agggcgaacg ctacgattgc ctggtgcggc aggtgaccaa ccggatgcgc cgggtccagc
     4081 agccgccggc caaggtcacc cgcctcgacc gggaggaggc ggatcgcaag atggccg
//
LOCUS       DASWNU010000071         8209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_80315, whole genome shotgun sequence.
ACCESSION   DASWNU010000071 DASWNU010000000
VERSION     DASWNU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80315"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VGB57_01420"
     CDS             <1..711
                     /locus_tag="VGB57_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY0130033.1"
                     /translation="ACRMIKSPAEIALMQKAADVTVAAYRHTYRRIEKGMSPSDIGAI
                     MNGATVALGGQPEFALVLLGEASAYPHGSGKPQAVRKSEVVLMDCGCTVHGYQSDISR
                     TFVFGEAGAEQRRVWSQMRKGQDIAFAAARVGAPAGSVDDAVRRQYEAWGYGPGYKLP
                     GTSHRTGHGIGLEGHEPVNFVHGETTRLAPGMCFSNEPGIYLPGKFGIRLEDCLYMTA
                     GGPRWFSQPPPSIDRPFG"
     gene            complement(799..2115)
                     /locus_tag="VGB57_01425"
     CDS             complement(799..2115)
                     /locus_tag="VGB57_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY0130034.1"
                     /translation="MAKRLTYWILFALVLGIVVGWGLNFLLGDGTPEGGPVVKEVARY
                     LSILTTIFLHLIKMIIAPLVFSTLVVGIAHMGDTAALGRVGAKALGWFVAASLVSLTL
                     GLILVTLFQPGTGLGLPLPPVDASAGIDRSAFDIVKFFGNIFPESMIRAMSENSILQI
                     VVFSLFVGVAITAVGEPARPLVRATEALVKVMLVITDYVMRFAPFAVFAAVASTVAQR
                     GPSILGTFGYFMGSFYVGLILLWVLLLGICFLIVGSRTRLLVRYIRDPIVLAFSTASS
                     EAAYPRTLEALDRFGVPPRIASFVLPLGYSFNLDGSMMYMTFATLFVAQAYGIELSMT
                     QQILMLLTLMITSKGVAGVPRASLVVITATMAQFNIPEAGLLLILAVDHFLDMGRSAT
                     NVVGNAVASVVVAKWEGDLDPVEPVDIEPPHAPAHVAPPKPGDEPF"
     gene            complement(2313..4331)
                     /locus_tag="VGB57_01430"
     CDS             complement(2313..4331)
                     /locus_tag="VGB57_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0130035.1"
                     /translation="MPDRSTGTRTIALVGPGGAGKTSLAEALLFAAGAVDRQGSVDAG
                     TSVGDSSPEARQRAGSTELNLMHFEFLGDRFAIVDSPGSVGFSADGALAVAAADLAVV
                     VVDPDTDRAILAEPALRQLESLGVPHLIFVNKIDQARGGIRALLEALQPMSSSPLVAR
                     QIPIREGEKVTGFVDLALERAFKYRPGKPSEQIEIPADLADRESQDRFHMLEQLADHD
                     DALLEQLLMDETPDQATIFADIARETKENLVVPVLFGSALNGFGVRRLMKALRHDAPA
                     PTAAAERLGAKGGCAFVFKVSHGGNMGRLALARVFGGPLSEGTELKTGSGDSVRVGTL
                     FAMQGEKPSKLGKAAIGDVVAIAKAEGVDAGEWLGAGGKAPELALPASNYALAIATSD
                     RKDDVRLSTALHKLVEEDPSLDWEQDEALHETRLKGISDEHLKVTLARLKRRYGVAVD
                     AHPPVVGYKESIRKPVTQRGRHKKQSGGHGQFGDVVIEIRPLTRGEGFKFDEKIHGGA
                     VPRQYIPAVEQGVRDALGKGPLGFPVVDVAVTLIDGSYHSVDSSELAFRTAGRIGMSE
                     ALALAQPHLLEPVHKVTVVTPGYATSRVTSAIASRRGQMLGMDSRAGWSRWDVVEALV
                     PAAELQGLDAELRSVSQGLATYEAKFDHLAELNGKLAEDVVQKVLEPA"
     gene            4618..5103
                     /locus_tag="VGB57_01435"
     CDS             4618..5103
                     /locus_tag="VGB57_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130036.1"
                     /translation="MPVSTTLPVMAGLSILGAALGITLGKSAIAEINPAYFNAPEDDF
                     HADLVPYRSGDWAQVHQAEYRAAQQPVLVGDLGGGCVGCRAYPVEYVPQHDPAVDRYV
                     EGGLLSAPEPTNVPVEIAAAPDPDRERIQRYASYPVSSEERPVEVAEAPAEEPAAPTG
                     L"
     gene            5241..7901
                     /gene="alaS"
                     /locus_tag="VGB57_01440"
     CDS             5241..7901
                     /gene="alaS"
                     /locus_tag="VGB57_01440"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0130037.1"
                     /translation="MTSTNDIRRSFLDYFGSSGHRVVPSAPLVPQNDPTLMFVNAGMV
                     PFKNVFTGLETRPWSTAASSQKCVRAGGKHNDLDNVGYTARHPTFFEMLGNFSFGDYF
                     KEQAITHAWTLLTREWGLSPDRLTATVYHTDDEAFGLWRKIAGLPESRIIRIPTKDNF
                     WAMGDSGPCGPCSEIFYDHGDHIPGGPPGSPDEDGDRFVEIWNLVFMQYEQEDNEIVR
                     ELPRPSIDTGMGLERIAAVLQGVHENYETDTFKALIAASSELTRTTAEGQRKASHRVI
                     ADHLRASGFLISDGVLPANEGRGYVLRRIMRRAMRHAHILGAAEPLMHRLVPSLVAEM
                     GAAYPELVRAQPLIEETLKLEETRFRQTLANGLRLLDEATGAMKPGDTLPGEVAFKLY
                     DTFGFPYDLTEDALRAQGYGVDRSGFDSAMGEQKAQARAAWKGSGQKASEDVWFDIAE
                     EQGSTEFTGYAGHEGEGVVVALVKDGVRVDRAATGDQVVILTNQTPFYGESGGQMGDT
                     GAVTSDKGLEAEVEDTSKPLGRLHAHHAVIRSGEVAVGDAVHLAVDAERRGRVRANHS
                     ATHLLHAALRHRLGGHVTQKGSLVAPDRFRFDFSHPKALTSEDIAAVEEEVNRHIREN
                     DAVTTRLMSPDEAIEAGAMALFGEKYGDEVRVLSMGRGSDASYSVELCGGTHVNALGD
                     IQLFKIVSESAVAAGVRRIEALTGEAARLWLTERENRLREAAAALKASPEEVPARVAA
                     LIDDRRRLERELAEAKKALAMGGGSKAEAQGPEQVGGHAFIGQVLEGMDPKGLRGLVD
                     DAKKRVGSGVVALVAVNDGRAAVAVGVTGDLTARRNAVDLVRKAVEAVGGQGGGGRPD
                     MAQGGGPDGSKAAAALDAVKAALTEETVAA"
     gene            complement(7941..>8209)
                     /locus_tag="VGB57_01445"
     CDS             complement(7941..>8209)
                     /locus_tag="VGB57_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130038.1"
                     /translation="IVAAEIDLINNRLLGTEQQKESVLPVLLEGEKNEALPPLVQGRI
                     HADFRNELDYFDIVFELILSHYELQSDDPLVTDLRESLKEPELR"
BASE COUNT         1393 a         2726 c         2840 g         1250 t
ORIGIN      
        1 gcctgccgga tgatcaagtc acccgccgag atcgctttga tgcagaaggc ggcggacgtg
       61 accgtcgcgg cttaccgcca cacctacagg cgcatcgaga aaggcatgag cccaagcgac
      121 atcggcgcga tcatgaacgg cgcaaccgtc gcgctcggcg ggcagccgga attcgcgctc
      181 gtcctgctcg gcgaggcgag cgcctacccg cacggcagcg gcaagccgca ggcggttcgg
      241 aaaagcgagg tggtgctgat ggactgcggc tgcacggtcc acggctacca gtccgacatt
      301 tcccgcacct tcgtcttcgg cgaggccggc gccgagcagc gcagggtctg gagccagatg
      361 cgcaagggcc aggacatcgc cttcgccgcc gcccgggtcg gcgctccggc cggaagcgtc
      421 gacgatgcgg tgcggcgcca gtacgaggcc tggggctacg gccccggcta caagctgccc
      481 ggcacctccc accgcaccgg ccacggcatc ggcctggagg ggcacgagcc ggtcaatttc
      541 gtccacggcg agacgaccag gctcgcgccg ggcatgtgct tctcgaacga gccgggtatc
      601 tacctgcccg ggaagttcgg aatccgcctg gaggactgcc tgtacatgac ggcgggcggc
      661 ccgcgctggt tctcgcagcc gccgccgagc atcgaccggc ctttcggctg actcgtcatc
      721 cccgcgaaag cggggatccc gcttcttttc ccgagccctt gaagaagcgg gattcccgct
      781 ttcgcgggaa tgacggagct agaaaggctc gtcccccggc tttggcggag cgacgtgcgc
      841 cggcgcatga ggaggctcga tgtcgaccgg ctcgaccgga tcgagatcgc cttcccattt
      901 cgccacgacc acgctcgcga ccgcattgcc gacgacgttg gtggccgagc ggcccatgtc
      961 gaggaaatgg tcgaccgcaa ggatcagaag cagcccggcc tccgggatgt tgaactgggc
     1021 catggtcgcg gtgatcacga ccaggctcgc ccggggcact ccggcgacgc ccttggaggt
     1081 gatcatgagc gtgagcagca tcaggatctg ctgggtcatc gacagctcga tgccataggc
     1141 ctgggcgacg aacagggtcg cgaaggtcat gtacatcatc gagccgtcga gattgaacga
     1201 gtaaccgagc ggaagcacga agctggcgat ccgcggcggc acgccgaagc ggtccagtgc
     1261 ctccagcgtg cgcgggtagg ccgcctccga cgatgcggtg gagaaagcga ggacgatggg
     1321 gtcgcggatg tagcggacga gaagccgggt ccggcttccg acgatcagga agcagatgcc
     1381 gagcagaagc acccagagca ggatcaggcc gacatagaag ctgcccatga aatagccgaa
     1441 ggtgccgagg atcgaaggcc cgcgctgggc gacggtggaa gcgaccgctg cgaagacggc
     1501 gaagggggcg aagcgcatca catagtcggt gatgacgagc atcaccttga ccagcgcctc
     1561 cgtcgcccgg accagcggcc gggccggctc gccgaccgcg gtgatcgcca ctccgacgaa
     1621 cagcgagaag acgacgatct ggaggatgct gttctccgac atcgcccgga tcatcgattc
     1681 cgggaagatg ttgccgaaga acttgacgat gtcgaaggcg gaacggtcga tccccgccga
     1741 ggcatcgacc ggcggcagcg gaaggccgag gccggtgccc ggctggaaca gcgtgaccag
     1801 gatgaggccg agcgtcagcg agacgaggct cgccgcgacg aaccagccga gcgccttggc
     1861 gcctacccgg ccgagcgccg cggtgtcccc catatgggct atgccgacga ccagggtcga
     1921 gaagaccagg ggcgcgatga tcatcttgat cagatggagg aagatcgtcg tcaggatgga
     1981 gagatagcgc gccacctcct tgaccaccgg cccgccctcg ggcgtgccgt cgccgagcag
     2041 gaagttcagg ccccagccga cgacgattcc gagtacgagg gcgaacagga tccagtaggt
     2101 gagacgtttg gccacgcgcg gctcctcgtg cttcaggtgc gcctaggaag tcgatagttg
     2161 ggcgcgggtc aaggtgcttg cctccccatg cgcagcaggg ggagggggac cccgcgtagc
     2221 ggggtggagg ggtaagtgga gcaggccgag ccagctaccc ctccaccacc cttcgggtgg
     2281 tccccctccc catcttcgat ggggaggcaa agttacgccg gctcgagcac cttctggacc
     2341 acgtcctccg cgagcttgcc gttgagctcg gcgagatggt cgaacttcgc ctcgtaggtc
     2401 gcgagcccct ggctcaccga ccgcagctcg gcgtcgagcc cctgaagctc ggccgccggc
     2461 accagcgcct cgaccacgtc ccagcgcgac cagccggccc ggctgtccat gccgagcatc
     2521 tggccgcggc ggctggcgat ggccgaggtg acccgcgaag tcgcatagcc cggagtgaca
     2581 accgtgacct tgtggaccgg ctcgagcaga tgcggctggg ccaaggcgag ggcctcgctc
     2641 atcccgatcc ggccggcggt gcggaaggcg agctcggagc tgtcgacact atggtagctg
     2701 ccgtcgatga gggtgaccgc gacgtcgacg accgggaagc cgagcggccc cttgcccaga
     2761 gcgtcgcgaa cgccctgctc gaccgcgggt atatattgcc tcggcaccgc tccgccgtgg
     2821 atcttctcgt cgaacttgaa gccctcgccg cgggtgagcg gcctgatctc gatgacgacg
     2881 tcgccgaact ggccgtggcc gcccgactgc ttcttgtggc ggccacgctg ggtcaccggt
     2941 ttgcggatcg attccttgta gccgacgacc ggcggatggg cgtcgacggc gactccgtag
     3001 cgccgcttca gccgcgccag ggtcaccttg agatgctcgt cgctgatccc cttgaggcga
     3061 gtctcgtgga gcgcctcgtc ctgctcccag tcgagcgagg gatcctcctc gaccaatttg
     3121 tggagtgcgg tcgacagtcg aacgtcgtcc ttgcggtcgc tggtggcgat ggcgagcgcg
     3181 taattggagg cgggcagggc cagctccggc gccttgccgc cggcgccgag ccattcgccg
     3241 gcgtcgaccc cttccgcctt ggcgatggcg acgacgtcgc cgattgcggc cttgcccagc
     3301 ttggacggct tctcgccctg catcgcgaac aaggttccca cccgaaccga gtcgccggag
     3361 ccggtcttca gctcggtgcc ttcgctgagc ggcccgccga agacccgtgc gagggcgagc
     3421 cgccccatat tgccgccgtg cgagaccttg aacacgaagg cgcagccgcc tttggcgccg
     3481 agccgctcgg ccgccgccgt gggcgccggg gcatcgtggc gaagcgcctt catcagccgg
     3541 cgcactccga agccgttgag ggccgagccg aacagcaccg gcacgacgag attctccttc
     3601 gtctcgcggg cgatgtcggc gaagatcgtc gcctggtcgg gagtctcgtc catcaaaagc
     3661 tgctcgagca gggcgtcgtc atggtcggcg agctgctcga gcatgtggaa gcggtcctgc
     3721 gactcgcggt cggcgagatc ggcggggatt tcgatctgct cggagggttt gccgggccgg
     3781 tatttgaagg cgcgctcgag cgcgaggtcg acgaagccgg tgaccttttc cccctcccgg
     3841 atcgggatct ggcgcgcgac caggggcgac gagctcatcg gctgaagcgc ttcgaggagc
     3901 gcccggatgc cgccccgggc ctggtcgatc ttgttgacga agatgaggtg ggggacgccg
     3961 agcgactcga gctgcctcag agccggttcg gcgaggatgg cccggtcggt atcgggatcg
     4021 acgacgacga cggcgagatc ggcggccgcc accgcgaggg caccgtcggc ggagaagccg
     4081 accgagccgg gcgagtcgac gattgcgaat cgatcgccga gaaattcgaa atgcatcagg
     4141 ttgagctcgg tcgagccggc gcgctgccgc gcctccgggc tcgaatcgcc gacgctggtg
     4201 ccggcgtcga cgctgccctg gcggtcgacc gccccggcgg cgaagagcag cgcctcggcg
     4261 aggctggtct tgcccgctcc gccgggtcca acgagggcga tggtcctggt gcctgtcgat
     4321 ctgtcgggca tgcgtctctc cttgccgttc cagggcgcga gccgcgttga agcgcgtccg
     4381 cgctcaatcc cgccagcgtt ccaccctttg aagcggaatc gcaagagggc aggtgaggga
     4441 tccccgtcat tgcgaggagt gaagcgacga agcaatccag cgggcctcgg gaggccgcgc
     4501 gggattgctt cgcttcgctc gcaatgacgg ggatccgcga gacagggaac accctcgccg
     4561 ggtcgtgggt ttaacctgtg ttgcggtctc agccgcttgt accagggagt gacgcccatg
     4621 cccgtctcga cgactctgcc ggtcatggcc ggactctcga tcctcggcgc cgcgcttgga
     4681 atcaccctcg gcaagtcggc catcgcagag atcaatcccg cttatttcaa cgcgccggag
     4741 gatgatttcc acgccgacct cgtgccctat cgaagcggcg actgggcgca ggttcaccag
     4801 gccgaatatc gcgccgccca gcagcccgtg ctggtcggcg atctcggcgg cggctgcgtc
     4861 ggctgccgcg cctatccggt ggaatatgtc ccgcagcacg atccggccgt cgaccgctat
     4921 gtcgagggcg gattgcttag cgccccggag cccaccaacg tcccggtcga gatcgcggcg
     4981 gcgcccgatc cggaccgcga gcggatccag cgttatgcca gctatccggt gagcagcgag
     5041 gaaaggccgg tcgaagtggc cgaagctccg gccgaagagc ctgccgcgcc gacggggctt
     5101 taatcccccg tcatcccggc gaaggccggg acccatgaac actgccggtg acagcgtcgc
     5161 acgacggtgt tcatggatcc cggatcaagt ccgggatgac ggctgttgcc gcagcgcagc
     5221 atgtggctta ccttaggccc atgacctcca ccaacgacat ccgccggtcg ttcctcgact
     5281 atttcggctc cagcgggcac cgggtcgtgc cctcggcgcc gctcgtcccg cagaacgacc
     5341 cgaccctgat gttcgtcaat gccgggatgg tgccgttcaa gaacgtcttc accggactcg
     5401 aaacccggcc ctggtcgacc gcggcctcgt cgcagaagtg cgtccgcgcc ggcggcaagc
     5461 ataacgatct cgacaatgtc ggctacaccg cccggcatcc caccttcttc gagatgctcg
     5521 gcaatttctc gttcggcgat tatttcaagg aacaggcgat cacccacgcc tggaccctgc
     5581 tgaccaggga atggggactg tctccggacc ggctgaccgc caccgtctac cacaccgacg
     5641 acgaggcgtt cggcctgtgg cgcaagatcg ccggccttcc cgagagccgg atcatccgga
     5701 tcccgaccaa ggacaatttc tgggcgatgg gcgacagcgg cccgtgcggg ccctgctcgg
     5761 agatattcta cgaccatggc gaccatatcc ccggcggccc gcccggcagc ccggacgagg
     5821 acggcgaccg cttcgtcgag atctggaacc tggtcttcat gcaatatgag caggaggaca
     5881 atgagatcgt ccgcgagctg cccaggccgt cgatcgacac cggcatgggg ctggagcgga
     5941 tcgccgccgt gctccagggc gtccatgaga attacgagac ggacacgttc aaggcactga
     6001 tcgccgcctc gagcgagctg acccggacga cggcggaagg gcagcgcaag gccagccacc
     6061 gggtcatcgc cgatcacctc cgcgcgtccg gcttcctgat ctcggacgga gtccttccag
     6121 ccaacgaggg caggggctat gtccttcgcc ggatcatgcg ccgggcgatg cgccacgccc
     6181 acatattggg cgcggccgag ccgctcatgc accggctggt gccgtcgctg gtcgccgaga
     6241 tgggcgccgc ctaccccgag ctggtccgcg cccagccgct gatcgaggag accctgaagc
     6301 tcgaggagac ccgcttccgg cagacgctcg ccaatgggct gaggctgctc gacgaggcga
     6361 ccggagcgat gaagcccggc gacacgcttc ccggcgaggt cgccttcaag ctctacgaca
     6421 cgttcggctt cccctacgat ctcaccgagg acgcgcttcg cgcgcagggc tatggagtcg
     6481 accgctccgg cttcgattcc gccatgggcg agcagaaggc gcaggcccga gccgcctgga
     6541 agggctcggg ccagaaggcg tcggaagacg tctggttcga catcgccgaa gagcagggct
     6601 cgaccgagtt cacaggctat gccgggcacg aaggggaggg cgtcgtcgtc gccctggtga
     6661 aggacggcgt ccgggtggac cgcgccgcga cgggcgacca ggtcgtcatc ctgaccaacc
     6721 agacgccctt ctacggcgag agcggcggcc agatgggcga caccggagcc gtgaccagcg
     6781 acaagggcct ggaggccgag gtcgaggaca cgtcgaagcc gctcgggcga ctccacgccc
     6841 accatgccgt gatccgctcc ggcgaggtcg cggtggggga cgccgtccac ctcgccgtcg
     6901 atgccgagcg gcgcgggcgg gtgcgggcca accacagcgc cacccatctc ctccacgcgg
     6961 ctctgcgcca ccggctcggc ggccacgtca cccagaaggg aagcctggtc gcgcccgacc
     7021 gcttccgctt cgacttctcg caccccaagg ctctgacgag cgaggacatc gccgcggtcg
     7081 aggaggaagt gaaccgccat atccgcgaga acgacgccgt caccacccgg ctgatgagcc
     7141 ccgacgaggc gatcgaggcc ggcgccatgg ctttgttcgg cgagaaatat ggcgacgagg
     7201 tccgggtcct ctcgatgggc cgcggctcgg acgccagcta ttcggtcgag ctatgcggcg
     7261 gcacccacgt caacgccctg ggcgacatcc agctcttcaa gatcgtctcg gagagcgcgg
     7321 tcgccgccgg agtccgccgg atcgaggccc tgaccggcga ggcggcgcgc ttatggctga
     7381 ccgagcgcga gaaccgcctc cgggaagcgg cggcggcgct caaggcctcg ccggaggagg
     7441 tgcccgcccg ggtcgccgca ctgatcgacg accgccgccg gctcgagcgc gagctcgccg
     7501 aggcgaagaa ggctctggcc atgggcggcg gctccaaggc cgaggcgcag ggtccggagc
     7561 aggtcggcgg ccacgccttc atcggccagg tgctcgaggg aatggatccc aaaggcctgc
     7621 gcggcttggt cgacgatgcc aagaagcggg tcggatcggg cgtcgttgcc ttggtcgccg
     7681 tcaacgacgg ccgggctgcc gtcgcggtcg gagtgaccgg cgatcttact gccaggcgaa
     7741 acgccgtcga cctggttcgt aaggcggtcg aagccgtcgg aggccagggc gggggagggc
     7801 gcccggacat ggctcagggc ggcggcccgg acggttcgaa ggcggcagcc gcgctcgatg
     7861 ccgtcaaggc cgcgctgacc gaagagacgg tcgcggctta acctcgccgt agcgtcgcgc
     7921 gaatagcgcc ccgcggcatc tcaacgcagc tcgggctcct tcagcgactc ccgaaggtcg
     7981 gtcaccaggg gatcgtcgga ctggagttca taatggctca ggatcagctc gaaaacgatg
     8041 tcgaaataat caagttcgtt ccggaagtcg gcatggatcc ggccttggac gaggggcggt
     8101 agcgcctcgt tcttttcccc ttcgagaagg acgggcagga cgctttcttt ttgttgctcc
     8161 gttccgagga ggcggttgtt gatcaggtca atctccgccg ccacgatat
//
LOCUS       DASWNU010000072         4625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_339876, whole genome shotgun sequence.
ACCESSION   DASWNU010000072 DASWNU010000000
VERSION     DASWNU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339876"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGB57_01450"
     CDS             <1..409
                     /locus_tag="VGB57_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0130039.1"
                     /translation="NRERSAAGIPALAWDPVLAAEAASWGEQLAASGEFEHDTDTDGD
                     DPQGENLWAGTSGAYAPEEMVGGWIEEKKHFRPGRFPDNSLTGDFADVGHYTQLMWRE
                     SDRVGCALAKGAEEEVLVCRYRTAGNVVGERPF"
     gene            complement(514..750)
                     /locus_tag="VGB57_01455"
     CDS             complement(514..750)
                     /locus_tag="VGB57_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130040.1"
                     /translation="MMKKLAFACAPILALAACAEEPEAVPTPDPALIERIVEANSAAA
                     ETPEKLEKADKLVAAVAHVEPDRVAGAAEKLLAR"
     gene            1467..2042
                     /locus_tag="VGB57_01460"
     CDS             1467..2042
                     /locus_tag="VGB57_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130041.1"
                     /translation="MSGTQDRKRRKNGQFAPRSGEETAVPKTPAKERRAKRSPERKAT
                     IKWTAAKEAIFFRELAMVCNVSAALRKAKLTGCSSLAYERRKTDGRFRAAWEGAVDQS
                     YAMLELEMLERVRLGDDRPPPATPAERRLRAIPTALALQLLKLYENRTKARAAAPAKA
                     AARLTRERGDALRKQLEQMLSDFNRRMGGEG"
     gene            2048..3538
                     /locus_tag="VGB57_01465"
     CDS             2048..3538
                     /locus_tag="VGB57_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY0130042.1"
                     /translation="MIERYARLSEEDRAGRFSPAERIAMMDPATRRAFLKILPRPVKQ
                     ALAGWHGRARPGQRAPEGDWRIWMIMGGRGYGKTLAGAQWVHQLAERPGLRIALVGPN
                     EDEARSVMVEGASGLLACASDDERPQWEPSLGRLSWPNGTQAFVHSGANPEALRGPEH
                     DYAWCDELAKWARAEASWDNLMFGLRRGALPRALVTTTPRPTPLMRKLAARGDVALTR
                     GRTADAALLAPSVADYLTGLYGGTRLGRQELDGELIDDVEGSLWPRDLIERCRFYPPR
                     AQHGEGDQVKPGGGVDGQVGGGSSENLDGLRHLPLHHPSGGPPPHLAMGRQIRRVVIG
                     VDPPASAEGDACGIVVCALLDDGIAHVVDDASVSGRSPEGWARAVAGAARKWGADRVV
                     AEGNQGGAMVASVLRAADVALPVRMVHASIGKCARAEPVAALFERGAAKFAGAFPALE
                     DELAGLIAGGGYDGPGRSPDRADAMVWAMTELMLGQRRADPRIRRL"
     gene            3645..>4625
                     /locus_tag="VGB57_01470"
     CDS             3645..>4625
                     /locus_tag="VGB57_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY0130043.1"
                     /translation="MKSWFGRKAAPAARPLLHRGYSGAAGVGDWPRSYEAQVREAYLG
                     NPVAQRAVRLVAESVAWAPVYATDGEAGAAASARALGLVSPALLDQAAAQLLLHGNAF
                     VQILLDAEAAPAELFALRPERVTVEADQGGWPAAYVYRAGEVKSRLAARDGLGRPALV
                     HIKAMHPLDDHYGLGCLGAASGAVAIHNSATRWNKALLDNAARPSGALSVEGGEGALG
                     PEQYERLREELELHFAGTANAGRPLLLEGGLKWQAMSLTPADMDFAGMKAAAAREIAL
                     AFGVPPMLLGLPGDSTYANYREANRALWRLTVLPLADTLLGAIAAALGAWW"
BASE COUNT          717 a         1422 c         1801 g          685 t
ORIGIN      
        1 caatcgcgag cgcagcgccg ccgggatccc ggccctggcc tgggaccccg tcctcgccgc
       61 ggaggccgct tcctggggcg agcagctcgc cgcgagcggc gagttcgagc acgataccga
      121 cacggacggc gacgacccgc agggcgagaa tctgtgggcg ggcacgagcg gcgcctatgc
      181 gcccgaggag atggtcggcg gctggatcga ggagaagaag catttccgcc ccggccgctt
      241 tcccgacaac agcctcaccg gcgatttcgc cgacgtcggc cactacaccc agttgatgtg
      301 gcgcgagagc gaccgggtcg gctgcgcgct tgcaaaaggg gcggaggaag aggtgctggt
      361 ctgccgctac cgaaccgccg gcaacgtggt gggcgagcgg cccttctagg aatgcccgtc
      421 atgctgaact tgtttcagca tccagcgatg aaccgcctcg ccggtggagg actggaccct
      481 ggaacgagtt cagggtgacg aactgcgact gcgtcagcgg gccagcagct tctcggcggc
      541 gccggcgacc cggtcgggct cgacatgggc gacggcggcg acgagcttgt cggctttctc
      601 gagcttctcg ggagtctcgg ccgcggccga attggcttcc acgatccgct cgatcaaggc
      661 gggatcgggc gtgggcacgg cctccggctc ttccgcgcag gcggcgaggg cgaggatcgg
      721 ggcgcaggca aaggcgagct ttttcatcat cttgcggcac caacgcgccg gatgcgcgga
      781 aggatgcgcc cggccgccac gtagaaatac gggaccgcgg cgggcgcttg cgctcaccgc
      841 tcgggcggcg accccgactt cgcgatcttc gcgttccttc gcgtgggaca gcagcgcggg
      901 ggagcgggga ggggctcggt ggacgatcga ggcgcgcctc ggccgaaccg ggccgaagca
      961 atggctcacg cgaagggact cgaaggtcgc gaagggaggc ggtcctttcc tggcctggat
     1021 gattcgcgcc gtggaatcca cgccagccga atcggggccg gaaacggcct ctgaaaataa
     1081 agtttttgcg cgaacgggct cgaaaaatag gatttgtgaa ggaaagtagc gggcccgaaa
     1141 aatcgcggat ttgtttcagc tggagcctcc gaaaaatagg atttgaccgg agcggtacaa
     1201 atggcttgac agcgtcacgc tgctcgggta ctctttagtc acgctgcaga attgtagcta
     1261 cggaagatcg agcagccggc gcggaccaac ccgcggcggc tttttctttg tgcgccgcgc
     1321 tgggcgccgg cggggccggg cccggtttcg acgagcgggc gcgcagcggc cgtcatgttt
     1381 tcgcgacctg aatcgtcccc ggcttttgcc ggggcggact ttcttcgtga aagcgtggcg
     1441 gccgtttcat tttgggggag ggtgagatga gcggaaccca ggatcggaag cgccggaaga
     1501 acgggcaatt cgcgccccgg agcggcgaag aaacagctgt cccgaagact ccggcgaagg
     1561 agcgccgcgc caagcgatcc ccggagcgca aggcgacgat caaatggacg gcggcgaagg
     1621 aggcgatctt tttccgggag ctggcgatgg tctgcaacgt cagcgcggcg cttcgcaagg
     1681 cgaagctgac gggctgcagc tcgctcgctt acgagcggcg caagacggac ggccgtttcc
     1741 gggccgcctg ggaaggggcg gtcgaccaga gctacgcgat gctcgagctg gaaatgctcg
     1801 agcgggtccg cctcggcgac gaccggccgc ctccggcgac cccggccgag aggcgcctga
     1861 gggcgatccc gaccgccctc gccctgcagt tgctcaagct ctacgagaac aggacgaagg
     1921 cgcgggcggc ggcgccggcc aaagcggcgg cacggctcac ccgggagcgc ggcgacgctc
     1981 ttcgcaagca gctcgagcag atgctttccg acttcaaccg ccgaatgggc ggggagggct
     2041 gagggccatg atcgaacgtt acgcgcgcct cagcgaagag gaccgggccg ggcgcttctc
     2101 gccggccgag cggatcgcga tgatggatcc ggcgactcgc cgggccttcc tgaagatcct
     2161 gccgcggccg gtgaagcagg cgctcgccgg ctggcacggc cgggcccggc cggggcagag
     2221 ggctcccgaa ggcgactggc ggatctggat gatcatgggc gggcgcggct acggcaagac
     2281 cctggccgga gcgcaatggg tgcaccagct cgccgagagg ccgggcctgc gcatcgcgct
     2341 cgtcggtccc aacgaggacg aggcgcgttc ggtgatggtc gagggggcgt cgggcctgct
     2401 cgcctgcgcc tcggacgacg agcggccgca atgggagccg tcgctggggc ggctgagctg
     2461 gccgaacgga acccaggcct tcgtccattc gggagccaat cccgaagcgc tgcgcgggcc
     2521 cgagcacgat tacgcctggt gcgacgagct cgccaaatgg gcgcgggccg aagcgagctg
     2581 ggacaatctg atgttcgggc ttcgccgcgg cgccctgccg cgggcgctgg tgacgacgac
     2641 tccgcggccg acgccgctga tgcgcaagct ggcggcgcgc ggcgacgtcg ccttgacccg
     2701 gggacggacg gcggacgccg cactgctggc gccgagcgtg gccgattatc tgaccggcct
     2761 ttacggcggg acgaggctcg gccggcagga gctggacggg gagctgatcg acgatgtcga
     2821 ggggagcttg tggccgaggg atttgatcga acggtgccgc ttttatcctc cccgtgcgca
     2881 gcacggggag ggggaccagg tgaaacctgg tggaggggta gatggccagg tgggtggagg
     2941 ttcgagcgag aacctcgacg gtctgcgcca cttacccctc caccaccctt cgggcggtcc
     3001 ccctccccat ctggcgatgg ggaggcaaat tcggcgggtc gtgatcggag tcgatccgcc
     3061 ggcttcggcg gagggggacg cctgcgggat cgtggtctgc gcgttgctcg acgacggcat
     3121 cgcccatgtc gtcgacgacg ccagcgtttc gggtcggagc ccggaagggt gggcccgggc
     3181 ggtggcgggg gcggctcgca aatggggcgc ggaccgggtg gtcgcggagg gcaatcaggg
     3241 cggggcaatg gtggcgagcg tgcttcgcgc cgccgacgtc gcgctgccgg tgaggatggt
     3301 ccacgcctcg atcggcaagt gcgcccgggc cgagccggtg gcggccttgt tcgaacgcgg
     3361 ggcggcgaag ttcgcgggcg cctttccggc gctcgaggac gagctcgccg ggctgatcgc
     3421 cggcggcggc tacgacgggc cggggcgatc gccggaccgg gccgacgcga tggtctgggc
     3481 gatgaccgag ctgatgctcg gacagaggcg cgccgatccg aggatccgga gattgtgagg
     3541 aaactcgtca tcccggcgga agccgggacc tcgggacgag aagtctcggc gggtctcgcc
     3601 gaggtcccgg ctttcgccgg gatgacggta tagggagaac atccatgaaa tcgtggttcg
     3661 gacggaaggc ggcgccggcg gcgcggccgt tgcttcatcg gggttattcg ggcgcggcgg
     3721 gggtcgggga ctggcctcgc agttacgagg cgcaggtgcg cgaggcctat ctcggcaatc
     3781 cggtggcgca aagggcggtt cggctggtgg cggagagcgt cgcctgggcg cccgtctatg
     3841 cgaccgacgg ggaggcggga gcggcggcct cggcgagggc gctcgggctg gtgagcccgg
     3901 ccttgctcga ccaggcggcg gcgcaattgc ttctccacgg caatgccttc gtccagatat
     3961 tgctcgacgc cgaagcggct ccggcggagc tgttcgcgct gaggccggag cgggtgacgg
     4021 tggaggcgga ccagggcggc tggccggcgg cctatgtcta ccgggcgggc gaggtgaagt
     4081 cgcggcttgc cgcccgcgac gggctcggcc ggccggccct cgtccatatc aaggcgatgc
     4141 acccgctcga cgaccattac gggctcggct gcctgggcgc ggcttcgggg gcggtggcga
     4201 tccacaattc ggcgaccagg tggaacaagg cgctgctcga caatgccgcc cggccttcgg
     4261 gcgcgctcag cgtcgagggc ggcgaggggg cgctcgggcc tgagcaatat gaaaggctcc
     4321 gcgaggagct ggagcttcat ttcgcgggca ccgccaatgc cgggcggccg ctgctgctcg
     4381 agggtgggct gaaatggcag gcgatgagcc tgacgcccgc ggacatggat ttcgcgggca
     4441 tgaaggcggc ggcggctcgg gagatcgcgc tcgccttcgg ggttccgccg atgcttctcg
     4501 ggcttccggg ggattcgacc tacgccaatt accgggaggc gaaccgggcg ctgtggcggc
     4561 tgaccgtgct tcccctcgcc gacacattgt tgggggcgat cgcggcggcg ctgggggcct
     4621 ggtgg
//
LOCUS       DASWNU010000073         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_288134, whole genome shotgun sequence.
ACCESSION   DASWNU010000073 DASWNU010000000
VERSION     DASWNU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288134"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VGB57_01475"
     CDS             complement(<1..322)
                     /locus_tag="VGB57_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0130044.1"
                     /translation="MNKEPRGRHLVAADRRFATGGGLLAHLLSGSFSRILGRIDGGLE
                     QGAIEAILPDGSRRILGGRRPGPRPVVRLHSWRALARLISSGSVGWYKSWEQGEWSSP
                     DPVPL"
     gene            520..774
                     /locus_tag="VGB57_01480"
     CDS             520..774
                     /locus_tag="VGB57_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130045.1"
                     /translation="MTRTRRTILALVALELMLAGLWFYLANMAATSPGASADSGRVIG
                     QTMGTVMGAVLGLSPLLYLLARSNDRKAAAARHSGEGRNP"
     gene            complement(843..1865)
                     /locus_tag="VGB57_01485"
     CDS             complement(843..1865)
                     /locus_tag="VGB57_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY0130046.1"
                     /translation="MNELTKLTAKDFATDQEVRWCPGCGDYAVLKAVQRTMPELGVRP
                     ENTVFVSGIGCSSRFPYYMETYGFHTIHGRAPSFATGIKLARPELDVWIITGDGDALS
                     IGGNHTMHVLRRNLDCQILLFNNEIYGLTKGQYSPTSRAGTRSPSTPFGSVDRPVSPC
                     AFALGSGARFIARAIDVHKNLPEVLKAAHAHKGASFVEIYQNCIVYNDDVFAPFTAKE
                     NSGQQLWLKAGEPMLFAGGARGIALDREALALRVVEVEGGDWQSAGVLVHDPRNRSVA
                     HMLVEMPFGAFPTALGVLYEDPAPTFESAVVRQNAEAAAGKKADLQALVSKGQSWMVT
                     KEPHVI"
     gene            complement(2002..3882)
                     /locus_tag="VGB57_01490"
     CDS             complement(2002..3882)
                     /locus_tag="VGB57_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY0130047.1"
                     /translation="MATATHYVTPEEAGADTPCEAVVVRFAGDSGDGMQLTGGQFTLS
                     TALAGNDLATFPDFPAEIRAPQGTTFGVSAFQINFGSAAIETAGDSPDVLVAMNPAAL
                     KTNVEDLKPGGLIIADEGEFGTRNLAKAGYGANPLDDGSLGKWQVIRFNISQLTLDAV
                     KPFGLGNKEALRCKNMWTLGLALWMFDRDRTPIVEWLRSKFAKNAELAEANIAALNAG
                     HAYGETAEIGGQIPQRHIDPAPAEPGLYRTVTGAESISLGLVAGAQLAGLPMFFGGYP
                     ITPASAILHHLSRLKEYGVTTFQAEDEIAAIGSAIGAAYAGSLGVTSSSGPGIALKME
                     AMGLAIMTELPLVIVNSQRGGPSTGLPTKTEQSDLYQAVYGRNGDAPLPVIAARSPAD
                     AFECAIEACRIAVQYMTPVMLLTDGYIANAAEPWRVPDMASFEPFPVRFHDEPPAEGE
                     AVNPYARDSKLARVWIRPGSPGLTHRIGGIEKNVGTGHIDYAPANHQAMTDIRADKVL
                     GVADSIPEQEVCLGAAGAKLAVVGWGSTFGPIHQAVRRAQGRGLDVAHIHIRHIWPLP
                     RNLGELLRSFDRIIVPEMNKGQLKTLLRDQYLVDARPVNKVSGQPFKIAEIEAAIEEA
                     LA"
BASE COUNT          661 a         1365 c         1444 g          705 t
ORIGIN      
        1 acaaaggcac gggatcgggc gacgaccatt ccccctgctc ccagcttttg taccagccga
       61 cggagcccga gctgatcagc cgggcgagcg cccgccagct gtgcaggcgg acgaccggcc
      121 gggggccggg ccggcgcccg ccgaggatcc gccgcgagcc gtcgggcagg atcgcctcga
      181 tcgccccctg ttccagccca ccgtcgatcc tcccgaggat tcgcgagaag ctccccgaca
      241 gcaggtgcgc gagaagcccg ccgccggtgg cgaaacggcg gtcggcggcg acgagatggc
      301 ggccgcgggg ctccttgttc atggcgcgct tggtggcggg aaaggggaaa ggacgcaact
      361 cccgtcgtcc aagtcccgtc attccggcga aggccggaat ccatgaacgc gaagggtgcc
      421 ggttttcagt ggaccgtgtt catggatccc ggccttcgcc gggatgacgg attattccgg
      481 acctgttatc aatccagccg ggattgatgg ggaaacgaca tgaccagaac cagacggacg
      541 atcctcgcgc tggtcgctct tgagctcatg ctcgccggcc tgtggttcta tctggcgaac
      601 atggccgcga catcgcccgg cgcgagcgcg gattccgggc gagtcatcgg gcagacgatg
      661 ggcacggtga tgggggcggt gctcggcctc tcgcccttgc tctacctcct cgcccggagc
      721 aacgaccgca aggcggcggc agcccgtcat tccggcgaag gccggaatcc atgaacacgg
      781 tcaggtgaaa accggcacgt tccgtgttca tgggtcccgg ccttcgccgg gatgacgcga
      841 cgtcagatga cgtgcggctc cttggtcacc atccagctct ggcccttgga gaccagagcc
      901 tggaggtccg ccttcttgcc ggcggccgct tccgcgttct gccgaaccac cgcgctctcg
      961 aaggtcggcg ccggatcctc gtagagcacc cccagggcgg tcgggaaggc gccgaacggc
     1021 atctcaacca gcatgtgggc caccgagcgg ttcctgggat cgtggacgag cacgccggcc
     1081 gactgccagt cgccgccttc gacctcgacg accctgagcg ccagagcctc gcggtcgagc
     1141 gcgatgccgc gggcgccgcc ggcgaacagc atcggctcgc ccgccttcag ccagagctgt
     1201 tgcccggaat tctccttggc ggtgaagggc gcgaagacgt cgtcattgta gacgatgcaa
     1261 ttctggtaga tctcgacgaa gctcgcgccc ttgtgggcgt gggcggcctt cagcacctcc
     1321 ggcaggttct tgtggacgtc gatcgcccgg gcgatgaagc gcgcgcccga gcccagggcg
     1381 aaggcgcagg ggctgaccgg ccggtcgacc gagccgaacg gggtggaagg gctgcgggtg
     1441 ccggcccggc tggtcggcga atactggccc ttggtgaggc cgtaaatctc gttgttgaac
     1501 agcaatatct ggcagtcgag gttgcgcctc agcacgtgca tcgtgtggtt gccgccgatc
     1561 gacagagcgt cgccgtcccc ggtgatgatc cacacgtcga gctcgggcct ggcgagcttg
     1621 atcccggtcg caaagctcgg cgcccggccg tggatggtgt ggaaaccgta ggtctccatg
     1681 taatagggaa agcggctcga gcagccgatg ccggacacga agacggtgtt ctcgggacga
     1741 acgccgagct cgggcatggt ccgctggact gccttcagaa ccgcatagtc gccacagccc
     1801 gggcaccagc gcacctcctg gtcggtggcg aaatccttgg cggtaagctt ggtcagttcg
     1861 ttcataagat cctcgtcgcc ccagcgaagg ctggggcccc agggggtgaa aagctcgggc
     1921 gggtgtagcg cgctccactt acggcggcgg tcttcacccg ccggggtccc agccttcgct
     1981 gggacgacgg cctcggtccc ctcatgccaa agcctcctcg atcgccgctt cgatctcggc
     2041 gatcttgaag ggctgtcccg aaaccttgtt caccggccgg gcgtcgacca gatactggtc
     2101 gcggagcagg gtcttcaact ggcccttgtt catttccgga acgatgatcc ggtcgaagga
     2161 tctcagcagc tcgcccagat tcctcggcag cggccagatg tggcggatgt ggatgtgggc
     2221 gacgtcgagg ccccggccct gcgcccgccg gacggcctgg tgaatggggc cgaaggtcga
     2281 gccccagccg accacggcga gcttcgcgcc cgcggcgccg aggcagacct cctgctcggg
     2341 gatcgaatcg gccactccaa gcaccttgtc ggcgcggata tcggtcatcg cctggtggtt
     2401 ggctggggca taatcgatat ggccggttcc gacattcttc tcgatcccgc cgatccggtg
     2461 ggtgaggccg gggcttccgg ggcggatcca gacccgggcg agcttggagt ccctcgcata
     2521 gggattgacc gcctcccctt ccgccggggg ctcgtcgtgg aagcggaccg ggaagggctc
     2581 gaagctggcc atgtccggca cccgccacgg ctcggcggca ttggcgatat agccgtcggt
     2641 cagaagcatg actggagtca tgtactggac cgcgatccgg cacgcctcga tcgcgcattc
     2701 gaaagcgtcg gcgggagagc gggcggcgat gacgggaagc ggagcgtcgc cgttgcggcc
     2761 gtagaccgcc tggtagagat cggactgctc ggtcttggtc ggaaggccgg tcgagggccc
     2821 gccgcgctgc gagttgacga tgacgagcgg gagctcggtc atgatggcga ggcccatcgc
     2881 ctccatcttg agcgcgatcc ccggccccga cgaggaggtg accccgaggc ttccggcgta
     2941 ggccgcaccg atcgccgatc cgatcgcggc gatctcgtcc tcggcctgga atgtggtgac
     3001 cccatattcc ttgaggcgcg agagatggtg gaggatcgcg gaggcgggcg tgatcggata
     3061 gccgccgaag aacatcggca ggccggcgag ctgtgccccg gcgaccagcc cgagcgagat
     3121 cgattcggcc ccggtgacgg tccggtacag gcccggctcg gccggcgccg gatcgatgtg
     3181 gcgctgcggg atctggccgc cgatctcggc ggtctcgcca taagcgtggc ctgcgttgag
     3241 ggccgcgata ttggcttcgg ccaattcggc attcttggcg aatttggacc gcagccactc
     3301 gacgatggga gtccggtcgc ggtcgaacat ccacagcgcc aggccgagcg tccacatgtt
     3361 cttgcagcgc agcgcctcct tgttgccgag gccgaagggc ttcaccgcgt cgagggtgag
     3421 ctgcgagatg ttgaagcgga tgacctgcca tttgcccagg ctgccgtcgt cgagcggatt
     3481 ggccccgtag cccgccttgg cgaggttgcg cgtcccgaac tcgccctcgt cggcgatgat
     3541 cagcccgccg ggcttcagat cctcgacgtt ggtcttgagc gccgccgggt tcatcgcgac
     3601 caggacgtcg ggcgaatcgc ccgccgtctc gatcgccgcc gagccgaaat tgatctggaa
     3661 ggcggagacc ccgaaggtcg tcccctgcgg cgcccggatc tcggccggga aatcgggaaa
     3721 ggtggcgaga tcgttgccgg cgagcgcggt cgacaaggtg aactggccgc cggtcagctg
     3781 cattccgtcg ccgctgtccc cggcgaagcg cacgaccacc gcttcgcacg gcgtgtcggc
     3841 gccggcttcc tcgggcgtca cataatgggt ggctgtcgcc aatctggctt cctttgtcgc
     3901 aggttgcccg aacatcctag cggggcgggc gcgaggcgac aactcgctgc gatgtcagaa
     3961 cgggcgcggc aaaattcccg tcgtcccggc gaaagccggg acctcgggga gacccgccga
     4021 gacttctcgc cctgaagtcc cgctttcggg ttcccatcaa agtactactt tgatggggtc
     4081 cctcccggga tgacggacgc acgccccctc agcgcattgt atcccctcgg tatcccctcg
     4141 gcgcttcaat tgccgcggct gatcccgcgg agcct
//
LOCUS       DASWNU010000074         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_106830, whole genome shotgun sequence.
ACCESSION   DASWNU010000074 DASWNU010000000
VERSION     DASWNU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106830"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VGB57_01495"
     CDS             <1..871
                     /locus_tag="VGB57_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0130048.1"
                     /translation="CFHVEGGRHAVVEEALRTSGARFVANDCGLSDTNRLWLVTGPNM
                     GGKSTFLRQNALIAVLAQAGAYVPATRATLGLVDRLFSRVGASDNLARGRSTFMVEMV
                     ETAAILSQATERSFVILDEVGRGTSTYDGLAIAWAVTEAIHDRNRCRCLFATHYHELT
                     RLAERLDALSLHHVRAREWKGDLVLLHEVSKGPADRSYGLAVAKLAGLPAPVLARARS
                     VLAKLEQGRAATGGIAAGLDDLPLFAAVAQQEEEAPDPLQAELAQVDADSLSPREALE
                     LVYRLKRLAAGEG"
     gene            871..>2041
                     /locus_tag="VGB57_01500"
     CDS             871..>2041
                     /locus_tag="VGB57_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEY0130049.1"
                     /translation="MPTRFAPVPDKRSIIDRRTLADSLASAPDTAAAILKQALTAGRA
                     EIARRLGDKPYAGSETAAAYAFLTDQIVRLAYDHAVATLPPPRRTDRLLLLAVGGYGR
                     GEMALHSDVDIAFVTPARPGRWARQAVEAVLYLLWDLGLKLGPSTRSVDELIKIAASD
                     HTVRTALLEARWLWGDEPLFDEVQGRFWKEVVAGKAKSFVTEKLEERNARHRRMGDSR
                     YVVEPNVKEGKGGLRDLHTLFWIGKYAYQVRSVGDLVGVGLLSETELHQFRRAERFLW
                     AVRCHLHLVAGRAEERLTFDYQREIAARMRYADRPGKPPVERFMRHYFLHAKTVGDLT
                     GVFLAHLDEKFAKKGGRFGLPTIRRRPRRLEGFVLDRGRLALPSDDYFREDPVRLL"
BASE COUNT          293 a          780 c          652 g          316 t
ORIGIN      
        1 ttgcttccat gtcgaaggtg gccgccacgc ggtggtcgag gaggctttgc gcaccagcgg
       61 cgcgcggttc gtcgccaacg attgcggcct ctccgacacc aatcgcctgt ggctggtcac
      121 cggcccgaac atgggcggca agtcgacctt ccttcgccag aacgccctca tcgccgtcct
      181 cgcccaggcc ggcgcctacg tccccgccac gcgcgcgacg ctgggcctcg tcgaccgcct
      241 gttcagccgc gtcggcgcct ccgacaacct cgcccgcggc cgctccacct tcatggtcga
      301 gatggtcgag accgccgcca ttctctccca ggcgaccgaa cggagcttcg tcatcctcga
      361 cgaggtcggg cggggcacct ctacctacga cggtctcgcc atcgcttggg cggtgaccga
      421 ggcgatccac gaccgcaacc gctgccgctg cctgttcgcc acccattatc acgagctgac
      481 gaggcttgcc gaacggctcg acgccctctc gctccatcat gtccgggccc gtgaatggaa
      541 gggcgatctc gtcctcctcc acgaagtgtc gaaagggccc gccgaccgaa gctacggcct
      601 cgccgtcgca aagctcgccg gcctccccgc cccggtcctc gcccgggccc gctcggtcct
      661 cgccaagcta gagcagggcc gtgccgccac cggcgggatc gccgccgggc tcgacgacct
      721 gcccctgttc gcggccgtcg cccagcagga ggaggaggcg cccgatccct tgcaggcgga
      781 gctggcccag gtggatgccg actcgctcag cccgcgcgag gcgctggagc tggtctaccg
      841 cctgaagcgg ctcgccgccg gcgagggctg atgccgacgc gcttcgcccc ggtccccgac
      901 aagcgttcga taatcgaccg ccgcaccctc gccgactcgc tcgcttcggc gcccgacacg
      961 gcagctgcga tcctcaagca ggcgctgacc gcggggcgcg ccgagatcgc ccgccgcctg
     1021 ggcgacaagc cctatgccgg gagcgagacc gccgccgctt atgcgttcct gaccgaccag
     1081 atcgtccgcc tcgcttatga ccatgcggtc gccaccctcc ccccgccccg ccggaccgac
     1141 cggctgcttc tgctcgccgt cggcggctac ggccggggcg agatggcgct ccattccgac
     1201 gtcgacatcg ccttcgtcac gcccgccagg cccggccgct gggcccggca ggcggtcgag
     1261 gccgtccttt acctgctttg ggatctcggc ctgaagctgg gcccctcgac ccgctcggtc
     1321 gacgagctga tcaagatcgc cgcctccgac cacacggtcc gcaccgccct gctcgaggcg
     1381 cgctggctgt ggggcgacga gcccttgttc gacgaggtcc aggggcgctt ctggaaggag
     1441 gtggtggcag gcaaggcgaa gagcttcgtc accgagaagc tggaggaacg caacgcccgc
     1501 caccggcgga tgggcgacag ccgctacgtc gtcgagccca acgtcaagga gggcaaaggg
     1561 ggcttgcgcg acctccacac gctcttctgg atcggcaaat atgcctatca ggtgcggagc
     1621 gtcggcgacc tggtcggggt cgggctgctg tccgagacgg aattgcacca gttccggcgg
     1681 gccgaacgct tcctctgggc ggtgcgctgc catctccacc tcgtcgccgg ccgggccgag
     1741 gagcgcctga ccttcgacta tcagcgcgag atcgctgccc gcatgcgcta cgccgaccgg
     1801 ccgggcaagc cgccggtcga gcgcttcatg cgccattact tccttcacgc caaaaccgtc
     1861 ggcgacctga ccggcgtctt cctcgcccat ctcgacgaga aattcgcgaa gaaaggcggc
     1921 cgcttcggcc ttccgacgat ccgccgccgg ccgagacggc tcgaaggctt cgtgctcgac
     1981 cgcggacgcc tcgctctgcc gagcgacgac tatttccgcg aggatccggt ccggctcctt
     2041 c
//
LOCUS       DASWNU010000075         6464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_418245, whole genome shotgun sequence.
ACCESSION   DASWNU010000075 DASWNU010000000
VERSION     DASWNU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418245"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3677)
                     /locus_tag="VGB57_01505"
     CDS             complement(<1..3677)
                     /locus_tag="VGB57_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130050.1"
                     /translation="MAILTVGAEGDYSTIQAAVNAANPGDTIVVGAGIYAEQVVVTGK
                     DNLTIKAVPGETVTIQAPADLVETARSISDREIHAVFTVKNSANVVLEGIDIDGAGAG
                     NTVDEGSGGGQANFYGVFYRDSSGGLVDVDVTRIRDQLIGGELSGVQRGVGVGTDNSS
                     PLAFSMTGGSISDFQKNATVFNGADLNVSGVAITGDGATASIAQNGIQVTNSTGTIAG
                     NTISGIGYSGPGGAYSGAVLAFGNTGLDIQDNVITGANLQDAAARVVGVYVFQNGAGG
                     APSSGGSISGNTISYVDEGIDVSGAITPDGILIEDNVITNIDGSDNDPVGVYFEPDPA
                     FATDYSIEGTEDDDVLTGGSGNDSLSGLGGNDMLTGGAGNDSLSGDGDTDTVAYGGPR
                     SGYAVATTTDPAGRVTGFTAVTDTDTGNGDDGADTLTGVEKLSFTGLTLDLADPVQLF
                     DASNQLVGTFGTIQAAIDAALDDYTIRVAAGSYDEDLVIDVGVAILGARTGVAVGGRD
                     AAGGTGETTVVGRAQVTATANVTLDGLRLLNDTSTTAPTLQLLTGGGATGHLVTNSIF
                     WSTIAGGANALDDRAISTVVIADGLITITDNLISGTSQSQFGSASWGRGLWFDGGGVD
                     LVFTGNTLEWTRTGLNLDMSGDSTADVSDNSFKGLGTGISVGVDAVGLTVADNDIERV
                     NEEFNFRNLTSGVTFDAGAAIDILTPVGDFNDPVAVLGGSGNDNFTGTDGVDVLDGNN
                     SPTNPAAADADSLDGAGGNDFLFGRGGNDTLDGGAGDDAMTGGTGDDVYVVDSAGDSV
                     AEAADAGTDEIRTDLASYSLAALPNIENLTGLGNVDQILTGNDAGNVIDGGAGSDSID
                     GGDGVDTARYSGAATIVETATGWTVTDAGGTDTLSNVEMVDDNAGGKTLLVGNGGYAT
                     IQDAVDAASSGDTILVSSGTYVEQVVVDNKDGLTIQAAPGADVTIQAPGDVVETARSI
                     GGREIHAVFTVIDSANVVLEGIDIDGAGAGNTVDEGSGGGQANFYGVFYRDSSGGLID
                     VDVEGVRDAALSGAQRGVGVGTDNSTPLAFSMTGGSISDFQKNATVFNGAILNVTGVA
                     VTGAGATAAIAQNGIQVTNSTGTISGNTISGFGYTGGGTVATGVLGFGNTDLNVTGNT
                     ITGANQATTAAAVVGIYILDFGTPNSGGSISGNTVDHADQGIVVRGAVTPNGIVIENN
                     DITNIDGTDPEAAGVDFQPDPA"
     gene            complement(3928..5997)
                     /locus_tag="VGB57_01510"
     CDS             complement(3928..5997)
                     /locus_tag="VGB57_01510"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0130051.1"
                     /translation="MASLPPEVDLNGAEGGHDSSVGYAEGDPVTPLAPEATVSDPDSA
                     DFDGGSLTVAFTSGGTADDQLRIAGGGFFVEEADLYYQDVAIGTITGGADGSTPLVVT
                     FNAGVDAAVAAALIRAIGYVTFSGAPIPGERQITFTLADGDGGTSIARVARVSVTSVE
                     TPPLAQDDMIATAEDMIATGSLFGDNGGGADSDPDGPALKVAEVNGSADNVDATFVLD
                     SGARLTVNADGSYTYDPNGQFDSLADSSTGAANSSVVDTFTYRLAGGNVAMVTVTVNG
                     VASPGDRLMGDEGDNFITGTPGPDIFVLDQGGADVVSGQGGADIFYFGGALDADDNVD
                     GGGGHDTLVLQGDYSEGLVLDGRVTGIEAVSILAGSNRAFGGSETELHDYAISIQDSA
                     FAAGLRVRINGSALLAGEDLSFDGSAETDATFLIYGGAGTDRLTGGQGNDIFFFDSGR
                     FAAGDTVAGGQGYDGLFLRGNYALDFGARGFADLITDIENLTLTSATDQRYARGGGTE
                     FDYSLFLADSNVGAGRALTVSGALLTGAETMVLDGSRETDGHLRLFGGAADDTLIGGA
                     NADLIHGNLGGDEISGGGGADLFRYQATAESVDGSADHINDFTPGTDRIELERIDADT
                     LTAGDQAFSWIGSEAFSGTAGELRAYESGRVWIVEGDVDGDGEADLVIALTLQGQAPL
                     GAGDFIL"
     gene            complement(6206..>6464)
                     /locus_tag="VGB57_01515"
     CDS             complement(6206..>6464)
                     /locus_tag="VGB57_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130052.1"
                     /translation="LDFTPGTDKIELDRIDANANVAGNQAFTWIGSSGFSGTAGELRA
                     ENLNNMGWFVEGDTDGNGAADFVVWLTLQGSTPLSSGDFIL"
BASE COUNT          985 a         2142 c         2213 g         1124 t
ORIGIN      
        1 gcgggatcgg gctggaagtc gacgcccgcc gcttccggat cggtgccgtc gatgttggtg
       61 atgtcgttat tctcgatgac gatgccgttc ggagtgactg cgccgcggac gacgatgccc
      121 tggtcggcat ggtcgaccgt gttgccgctg atcgagccgc cgctgttcgg ggttccgaag
      181 tcgaggatgt agatgccgac cacggcggcg gccgtcgtcg cctggttcgc gccggtgatc
      241 gtgttgccgg tgacgttgag atcggtgttg ccgaagccga gcacgcccgt cgcgacggtg
      301 ccgccgccgg tatagccgaa gccgctgatc gtgttgccgg agatggtgcc ggtcgagttg
      361 gtgacctgga ttccgttctg ggcgatggcc gcggtggcgc cggcgccggt gacggcgacc
      421 ccggtgacgt tcaggatggc gccgttgaag acggtcgcgt tcttctggaa gtccgagatc
      481 gagccgccgg tcatgctgaa ggccagcgga gtcgaattgt cggtgcccac gccgaccccg
      541 cgctgggctc cgctgagcgc ggcgtcgcga acgccctcga catcgacgtc gatgaggccg
      601 cccgagctgt cgcggtagaa gacgccgtag aaattggcct gcccgccgcc gctgccctcg
      661 tcgaccgtat tgccggcgcc ggcgccgtcg atgtcgatcc cctcgaggac gacattggcc
      721 gaatcgatga ccgtgaagac ggcgtggatc tcgcggccgc cgatcgagcg ggcggtctcg
      781 acgacgtcgc ccggcgcctg gatggtcaca tccgcccccg gcgccgcttg gatggtcaga
      841 ccgtccttat tatcgacgac gacctgctcg acataggtgc ccgacgatac gaggatggtg
      901 tcgccgctcg aggcggcatc gaccgcgtcc tggatggtcg cgtagccgcc attgccgacg
      961 agcagggtct tgccgcccgc attgtcgtcg accatctcga cgttgctgag cgtgtcggtg
     1021 ccgcccgcgt ccgtgacggt ccagccggtc gcggtttcga cgatggtcgc ggcgcccgaa
     1081 tagcgcgccg tgtcgacccc gtcgccgccg tcgatcgagt cgctgccggc gccgccgtcg
     1141 atgacattgc cggcatcgtt gccggtgagg atctggtcga cattgccgag acccgtgaga
     1201 ttctcgatgt tcggcagagc ggcaaggctg tagctggcaa gatcggtgcg gatctcgtcg
     1261 gtgcccgcgt ccgcggcctc ggcgaccgag tcgcctgcgc tgtcgacgac atagacgtcg
     1321 tcgccggtac cgccggtcat ggcatcgtcg ccggcgccgc cgtcgagcgt gtcgttgccg
     1381 ccgcggccga acaggaagtc gttgccgccg gcgccgtcga gcgagtcggc atcggccgcc
     1441 gccgggttgg tcgggctgtt gtttccgtcg agcacgtcca ccccgtcggt gccggtgaaa
     1501 ttgtcgttgc ccgagccgcc gagcaccgcc accggatcgt tgaagtcgcc gaccggggtc
     1561 aaaatatcga tcgcggcgcc ggcgtcgaag gtgacgccgc tggtgagatt gcggaagttg
     1621 aactcctcgt tgacccgctc gatatcgttg tccgcgacgg taaggccgac cgcatcgacg
     1681 cccaccgaga tgccggtgcc aaggcccttg aagctgttgt ccgagacgtc ggcggtcgaa
     1741 tcgccggaca tgtcgaggtt gaggccggtg cgcgtccatt ccagcgtgtt gccggtgaag
     1801 acgagatcga ccccgccgcc gtcgaaccac aggccgcggc cccaggaggc gcttccgaac
     1861 tggctctgcg acgtccccga gatgaggttg tcggtgatcg tgatcaggcc gtcggcgatg
     1921 accacggtgg agatggcccg atcgtcgagg gcattcgcgc cgccggcgat ggtcgaccag
     1981 aagatcgagt tggtgacgag gtggccggtg gcgccgccgc cggtcaggag ctgcagggtc
     2041 ggggcggtcg tgctggtgtc gttgagcaag cgcaggccgt cgagagtgac gttggccgtt
     2101 gcggtgacct gggcgcggcc gacgacggtc gtctcgccgg tgccgcccgc ggcgtcccgc
     2161 ccgccgaccg ctacgcccgt ccgggcgccg aggatggcga ctccgacatc gatgacgaga
     2221 tcctcgtcat agctgccggc cgcgacccgg atcgtgtagt cgtccaaagc cgcatcgatc
     2281 gcggcctgga tcgtgccgaa cgttccgacc agctggttgc tggcgtcgaa cagctgcacg
     2341 gggtcggcga ggtcgagcgt caggccggtg aaggagagct tctccacgcc ggtgagcgtg
     2401 tcggcaccgt catcgccgtt gccggtatcg gtgtcggtga cggcggtgaa gccggtgacc
     2461 cggccggccg ggtcggtggt cgtggccacc gcatagccgg agcgggggcc gccataggcg
     2521 accgtgtcgg tgtcgccgtc gccgctgagg ctgtcgttgc cggcgccgcc ggtcagcata
     2581 tcgtttccgc cgaggccgga caggctgtca ttgccggacc cgccagtgag gacgtcgtcg
     2641 tcttccgtgc cctcgatcga atagtcggtc gcgaacgccg gatcgggctc gaaatagact
     2701 ccgaccgggt cgttgtcgga cccgtcgata ttggtgatga cattgtcctc gatgaggatt
     2761 ccgtccggcg tgatggcgcc gctgacgtcg atcccctcgt cgacataaga gatggtgttg
     2821 ccgctgatcg agccgccgct gctcggcgct ccgccggcgc cgttctggaa gacgtagacg
     2881 ccgaccaccc gagccgccgc atcctggaga ttcgcgccgg tgatgacatt gtcctggatg
     2941 tcgagaccgg tattgccgaa ggcgagcacg gcgccggaat aggcgccgcc ggggccggaa
     3001 taaccgatcc cgctgatcgt gtttcccgca atcgttccag tcgagttggt tacctggatt
     3061 ccgttctggg cgatggaagc ggtggcgccg tcgcccgtaa tggcgacccc gctgacgttg
     3121 aggtcggcgc cgttgaacac ggtcgcgttc ttctggaagt cgctgatcga ccccccggtc
     3181 atgctgaacg ccaacgggct cgaattgtcg gtgccgactc cgacgccacg ctggactccg
     3241 ctcagctcgc cgccgatcaa ctggtcgcga atgcgggtga cgtcgacgtc gaccaggccg
     3301 cccgagctgt cgcggtagaa gaccccgtag aaattggcct gcccgccgcc gctgccctcg
     3361 tcgaccgtat tgcccgcgcc ggcgccgtcg atgtcgatcc cttcgaggac gacgttggcc
     3421 gaattcttga cggtgaagac ggcatggatc tcgcggtcgc tgatcgatcg ggccgtttcg
     3481 acaaggtctg ccggcgcctg gatcgtgacc gtctcaccgg ggacggcctt gatggtcaga
     3541 ttatctttgc cggtgacgac gacctgctca gcgtagatgc cggcgccgac gacgatcgtg
     3601 tcgcccgggt tcgccgcgtt gaccgctgcc tgaatcgtgg aatagtcgcc ctccgcgcct
     3661 accgtcaaaa tagccacatc cgccccctct ggaaaaaggg ccaggccata catccgaatc
     3721 agatgcagcc gcttcccccc gggtggatgt gtgaactcgc tcaccgcgcg agtcaaacag
     3781 cattaagaaa gacttaatcc tttcaagggc catattttcg ccaacccggc aactgaatat
     3841 gcatccggaa tgggaacaaa gcaggcggag tgacgggacc tctcccgtca ttccttcccg
     3901 tcatgccggt gaaagccggg atgacggtta aaggataaag tcgcccgcgc cgagcggagc
     3961 ctgtccctgc agggtcaggg cgatgacgag gtccgcctcg ccgtcgccgt cgacgtcgcc
     4021 ctcgacgatc cagacccggc cggactcata ggccctgagc tcccccgccg tgccgctgaa
     4081 cgcctcggag ccgatccagc tgaacgcctg gtcgcccgcc gtcagagtgt cggcgtcgat
     4141 ccgctcgagc tcgatcctgt ccgtccccgg cgtgaaatcg ttgatatgat cggccgagcc
     4201 gtccaccgat tccgccgtcg cctgatagcg gaacaagtcg gcgccgccgc cgccgctgat
     4261 ctcgtcgccg ccgagattgc cgtggatcag gtcggcattg gcgccgccga tcagagtgtc
     4321 gtcggccgcg ccgccgaaca gccgaagatg cccgtccgtc tcgcggctgc cgtccaggac
     4381 catcgtctcg gcccccgtca gcaaggcgcc cgagacggtc agggcgcgcc cggccccgac
     4441 gttcgagtcg gcgaggaaca ggctatagtc gaactcggtg ccgccgcccc tggcatagcg
     4501 ctggtcggtc gcggaggtaa gggtcaggtt ctcgatgtcg gtgatcaggt cggcaaaccc
     4561 gcgagcgccg aagtcgagcg cgtaattgcc gcgaaggaag aggccgtcat agccctgccc
     4621 gccggccacc gtgtcgcccg cggcgaagcg gcccgagtcg aagaagaaga tatcgttgcc
     4681 ctggccgccg gtcagcctgt ccgttccggc cccgccgtag atcaggaagg tcgcatcggt
     4741 ctccgccgag ccgtcgaagc tgagatcctc ccccgccagc agggccgatc cgttgatcct
     4801 gacccgcaag ccggcggcga aagcggaatc ctggatcgag atcgcgtaat cgtgaagctc
     4861 ggtttccgaa cctccgaacg cccggttgga gccggccagg atcgagaccg cctcgatccc
     4921 ggtgactcgc ccgtcgagga cgagcccctc gctataatcc ccctgcagga cgagggtgtc
     4981 gtggccgccg ccgccgtcga cattgtcgtc cgcgtccagc gctccgccga aatagaagat
     5041 gtccgcgccg ccctggccgc tcacgacgtc ggcgccgccc tgatcgagca cgaagatgtc
     5101 ggggcccggc gtgccggtga tgaaattgtc gccctcgtcg cccatcagcc ggtcgcccgg
     5161 gctggcgact ccgttcaccg tcaccgtcac catggcgacg ttgccgcccg ccagcctgta
     5221 ggtgaaggtg tcgacaacgg atgaattggc ggcgccggtg ctgctgtcgg cgaggctgtc
     5281 gaactggccg ttcggatcgt aggtgtagga gccgtctgcg ttgacggtga gcctcgcccc
     5341 ggaatcgagg acgaaggtgg cgtcgacatt gtcggccgaa ccgttcactt ccgcgacctt
     5401 cagggccggg ccgtccggat cgctgtcggc gccgccgcca ttgtcgccga aaaggctgcc
     5461 ggtcgcgatc atatcctcgg cggtggcgat catgtcgtcc tgcgcgagcg gcggggtttc
     5521 gacggaggtg acgctgaccc tggccacccg cgcgatgctg gtgccgccgt cgccgtcggc
     5581 gagcgtgaag gtgatctgcc gctctccggg aatgggcgct ccggagaagg tgacgtagcc
     5641 gatggcgcgg atcaaagccg cggcgacggc cgcatcgacg ccggcgttga acgtcacgac
     5701 cagcggcgtc gagccatcgg cgcccccggt gatcgtgccg atcgcaacgt cctgataata
     5761 gaggtcggcc tcctcgacga agaagccgcc gccggcgatc cggagctggt cgtcggccgt
     5821 gcctccggag gtgaaggcga cggtcaggct cccaccgtcg aaatcggccg aatccgggtc
     5881 gctcaccgtc gcttcggggg cgagcggcgt gaccggatcg ccctcggcat agccgacgct
     5941 cgaatcatgg ccgccttcgg cgccgttcag atccacctcg ggtggaaggc tcgccatatc
     6001 tgttctcccc ggcggcgccg gagaggccgc cttccggtaa agccctgaca gaccgcaagc
     6061 ggcgacgcaa cgcccggcgc ccgctctgcg gccatccggg ctccatgagc ttcgccactc
     6121 ccgtcatccc ggcgaaagcc gggacctcgg gcagaatgag gctccgcccg ataagccgag
     6181 gtcccggctt ccgccgggac gacggtcaaa ggatgaagtc gcccgagctc agcggagtcg
     6241 atccctgcag ggtgagccag acaacgaagt cggcggcgcc gttgccgtcg gtgtcgccct
     6301 cgacgaacca gcccatgttg ttgagattct cggctcgaag ctcgcccgcc gttccgctga
     6361 acccgctcga tccgatccag gtgaaggcct ggttgcccgc cacgttggcg ttggcgtcga
     6421 tgcggtcgag ctcgatcttg tccgtccccg gggtgaagtc gagg
//
LOCUS       DASWNU010000076         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_106849, whole genome shotgun sequence.
ACCESSION   DASWNU010000076 DASWNU010000000
VERSION     DASWNU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106849"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..842
                     /locus_tag="VGB57_01520"
     CDS             93..842
                     /locus_tag="VGB57_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0130053.1"
                     /translation="MTKTAFITGATAGFGAAAVRRFAQAGWKVVGTGRRRERLDGLAR
                     ELGEAFLPLEIDMRDLAALQGAAEALPESFRGIDLLVNNAGLAPPLEPLQESRLEPAL
                     QAIDTNVTGLVVLTRSLLPILIERRGAVINLSSVAATYPYRGGAVYGGTKAFVSQFSL
                     DLRSDLHGTGVRVTSIEPGMAETEFTLVRTGGDQEASDTLYRGVDPMTAEDIAETLFW
                     VATLPPHLNINAIELMPVSQSFAGFQVARQQ"
     gene            1028..1264
                     /locus_tag="VGB57_01525"
     CDS             1028..1264
                     /locus_tag="VGB57_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130054.1"
                     /translation="MAVSPTRPATTVSGGSVVVTVPGKGVNNLAAVNKIVANTLGRLG
                     CPACLSGFDIRFKQVRDLVVNPQTFEVDELQIRG"
     gene            1261..2643
                     /locus_tag="VGB57_01530"
     CDS             1261..2643
                     /locus_tag="VGB57_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEY0130055.1"
                     /translation="MSPKLGRLGVGAVYWPSLHNLFEDHPDLIQVAEAEPSSFWIKAP
                     GPAGALRSSLDVLERLAELPQAKLLHGVGYPVGGTRCDQSEPVAELRRWAQRLGVAWT
                     SEHLSFNETDAGAAGFLLPPCQSDSGVAVAAANIRSRAALLDTPFAYETGDNYLPRQD
                     GEMADGAFFAAVAEAADCRILLDLHNLWANERNGRGRVLDVIAALPLERVCEIHLAGG
                     LEKDGFWLDAHSGPVPPELLALAAEIMPDLPAVGAILFELAPQHLGNVDRAGFLRQIE
                     ALHRLWERRGKAALPETRGPASFGRDINPAAWEACLVQALAAPPAESDPPAIGLYRSL
                     VASFRNGALADLLPHSLNLIAIGRGQARLEAILADYGDSTPPQLYPAHEALQFADWLA
                     ANPVDLPYLHDMLTFESAIVRFASDGRGGEVVLEHDPSIIAAAVAAGRSPTQVPAGDY
                     RLVIGATAQA"
BASE COUNT          421 a          957 c          855 g          448 t
ORIGIN      
        1 ctcggcctgc ccggcttcgc ctatgacgaa acggccgacc ggcgatcctg ggcggcgatg
       61 ctcaacctgt tcgaggaagt tctggagacc gcatgaccaa gaccgccttc atcaccggag
      121 ccaccgccgg cttcggcgcc gccgccgtcc gccgcttcgc gcaggcgggc tggaaggtgg
      181 tcggcaccgg ccgccgccgc gagcggctcg acgggctcgc tcgggagctc ggcgaagcct
      241 tcctgccgct cgaaatcgac atgcgcgatc tcgccgcgct gcagggggcg gccgaggcgc
      301 tgccggagtc gttccgcgga atcgacctcc tcgtcaacaa tgccggcctg gcgccgccgc
      361 tcgagcctct tcaggagtcg cggctggagc ctgcgctgca ggcgatcgac accaatgtca
      421 ccgggcttgt ggtcctcacc cggtccctcc tccccatttt gatcgagcgc aggggcgcgg
      481 tgatcaacct gagctcggtc gccgccacct acccgtatcg cggcggggcg gtttatggcg
      541 gcaccaaggc gttcgtcagc cagttctcgc tggacctgcg ctccgacctg cacggcaccg
      601 gcgtgcgggt gacctcgatc gagcccggca tggccgagac cgaattcacc ctcgtccgaa
      661 ccggcggcga ccaggaagcc tcggacacac tctaccgcgg cgtcgacccg atgacggcag
      721 aggacatcgc cgaaaccctg ttctgggtcg ccaccctccc gccccacctc aacatcaatg
      781 caatcgagct gatgccggtc agccagagct tcgcgggatt tcaggtggcg aggcagcaat
      841 aatgcccccg cgcggtatta aagccaacaa tagagtatct tggaccggga ttgttacgcc
      901 tccgtctccg atgttatctt tacctccccg actcggggca agcattgttc ctgacgagcg
      961 ataaagctcg aacgtcgagt ggagtcgccg ccgaggaaca gcttcacgtg gaaagaggag
     1021 aatgaggatg gctgttagtc cgacacgccc cgccaccacc gtcagtggcg gctccgtcgt
     1081 ggtcaccgtc cccggcaaag gggtcaacaa tctcgccgcc gtcaacaaga tcgtggccaa
     1141 tacgctgggc cggctcggct gccccgcctg cctttcgggc ttcgacatcc gcttcaagca
     1201 ggtgcgggat ctggtggtca atccgcagac cttcgaagtg gacgagctgc aaataagggg
     1261 atgagcccga agctcggccg gttgggagtg ggggccgtct actggccctc actccataat
     1321 ctcttcgagg atcatccgga cctcatccag gtcgccgagg ccgagccgtc cagcttctgg
     1381 ataaaggctc cgggccccgc gggcgcgttg cgatccagcc tcgacgtgct ggagcgcctg
     1441 gccgagcttc cacaggcaaa gcttctccac ggagtgggct accccgtcgg cggcacccgc
     1501 tgcgaccaga gcgagcctgt cgcggagctt cggcgctggg cgcagcggct cggagtcgcc
     1561 tggaccagcg agcatctcag cttcaacgag acggacgccg gcgccgccgg cttccttctg
     1621 cccccttgcc agtcggactc cggggtcgcg gtcgccgccg cgaacatccg gtcccgggcc
     1681 gcccttctcg acacgccttt cgcctacgag acgggcgaca attacctgcc gcgacaggat
     1741 ggggagatgg ccgacggcgc tttcttcgcc gccgtggccg aagcggccga ctgccggatc
     1801 ctgctcgacc tgcacaatct gtgggccaac gaacgcaatg gccgcggccg ggtcctcgac
     1861 gtgatcgccg ccttgccgct ggagagggtg tgcgaaatcc acctggccgg cggactcgag
     1921 aaggacggct tctggcttga cgcccattcg ggcccggtgc cgccggagct gcttgccctc
     1981 gcagccgaga tcatgcccga cctcccggcc gtcggagcga tcctgttcga gctcgcgccg
     2041 cagcacctcg gcaatgtcga ccgggccggc ttcctccggc agatcgaggc gcttcatcgt
     2101 ctatgggagc ggcgcggcaa ggcggcattg cctgaaaccc ggggaccggc ttcgttcggg
     2161 cgggacatca acccggcagc gtgggaggcc tgcctcgtcc aggcgctcgc cgcacctccc
     2221 gccgaatccg atccgccggc gatcggcctg taccggtcgc tcgtcgcgag cttccgcaac
     2281 ggcgcgctcg ccgatctcct gcctcacagc ctgaacctga tcgcgatcgg ccgggggcag
     2341 gctcgcctcg aagcgattct cgcggattat ggcgattcga ctccgccgca gctctatccc
     2401 gcgcacgaag cgcttcaatt cgccgactgg ctggccgcca atccggtcga ccttccctat
     2461 ctccacgaca tgctgacctt cgaatccgcc atcgtccgct tcgccagcga cggacggggc
     2521 ggggaggtgg tcctggagca cgacccttcg atcatcgccg ccgccgtcgc cgccgggcgt
     2581 tccccgaccc aggttccggc tggggactat cgactggtca tcggagccac ggcgcaggct
     2641 tgacctcccg gccgggccgg tgcttacgcc tcgcgccatg a
//
LOCUS       DASWNU010000077         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_158362, whole genome shotgun sequence.
ACCESSION   DASWNU010000077 DASWNU010000000
VERSION     DASWNU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158362"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..451
                     /locus_tag="VGB57_01535"
     CDS             134..451
                     /locus_tag="VGB57_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY0130056.1"
                     /translation="MQQPIKVDLPHKLGRDEARRRIANNIHKLRDHIPGGAAQVDSNW
                     NGDELDLTVHAMGQAVETRIGVEETKVRVEVLLPGMLALFAGPIEAMLQKKGNVLLED
                     KRD"
     gene            579..1883
                     /locus_tag="VGB57_01540"
     CDS             579..1883
                     /locus_tag="VGB57_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0130057.1"
                     /translation="MRHFAVVGSGPAGFYTAEALAKAYGAEARIDILDRYPVPYGLIR
                     FGVAPDHQSLKAVSKRYEKVAETDGVGFIGNVAIGRDVSVAELLEHYDAAILAIGAPH
                     DRKLGIPGEELPGVIGSAEFVGWYNGHPDFADLDPPLDGTHAAIIGNGNVALDCARIL
                     SKTRAEFESSDIVGHALEALDRSAIRIITILGRRGPHQIAMTPKELGELGHLEAAVPI
                     VDLSDFPPVEADDALEPGQRKSVTILRDFANIRSNKPKAMVFDFFAKPVSIEGGDRAE
                     HLVVERTELDEKGGARGTGETYEVPASLVVSCIGYSTPPIEGVPYDDRGGKFLNETGK
                     IADRLYCVGWARRGPTGTIGTNRPDGYEVAEAVAAAMPPGSTGDRAGAEGLRRLFTDR
                     SINATDFTDWRKIEAAEEAAARSGSPREKMVRVEQWLQTLGH"
BASE COUNT          364 a          708 c          658 g          302 t
ORIGIN      
        1 cgttcggcgg gggatagacg aacgcaggct gcgccgaagc ggccatcgat agtgcaagca
       61 gcaagctcat ccgggcagca taatggacgg gcgcagcttg aaaagcgatg aacggcagcc
      121 cactctcggc tccatgcaac agccgatcaa ggtcgacctg ccgcacaagc tgggccgcga
      181 cgaggcccgg cggcgcatcg ccaacaacat ccacaagctc agggaccaca tcccgggcgg
      241 cgccgcgcag gtcgattcga actggaacgg cgacgagctc gacctcaccg tccacgcgat
      301 gggccaggcg gtcgagacca ggatcggggt cgaggagacc aaggtccggg tcgaagtgct
      361 cctgccggga atgctggctt tgttcgccgg cccgatcgaa gcgatgcttc agaagaaggg
      421 caacgtcctc ctcgaagaca agcgcgacta agcaacagac cctcaccctc cgtcccccgg
      481 atcaagtccg gggtcctcct ccctctcccg cgcgcgggag agggttgggg tgagggcttc
      541 ttgccgttat gccctcccga ttgggacggg agcaaaggat gcggcatttc gcggtggtcg
      601 gctcgggccc ggcgggcttc tacaccgccg aggcgctggc caaggcgtac ggggctgaag
      661 cccggatcga catcctcgac cgctaccccg tgccctacgg gctcattcgc ttcggggtcg
      721 cgcccgacca ccagtcgctc aaagccgtct ccaagcgcta cgagaaggtc gccgagaccg
      781 acggcgtcgg cttcatcggc aatgtcgcga tcggccgcga cgtctccgtc gccgagctgc
      841 tcgagcatta cgacgccgcg atcctggcga tcggcgcgcc gcacgaccgc aagctcggca
      901 tccccggcga ggaattgccc ggagtgatcg gctcggccga gttcgtcggc tggtacaacg
      961 gccacccaga tttcgccgac ctcgatccgc cgctcgacgg cacccacgcg gcgatcatcg
     1021 gaaacggcaa tgtcgcgctc gattgcgcgc ggatcctgtc caagacgcgg gccgagttcg
     1081 aaagctcgga catcgtcggc cacgcgctcg aggcgctcga ccggtcggcg atccggatca
     1141 tcaccatcct cggccggcgc ggcccgcacc agatcgccat gacccccaag gagcttggtg
     1201 agctcggcca tctcgaagcc gccgtgccga tcgtcgacct cagtgatttc ccgccggtcg
     1261 aggccgatga cgcgctcgag cccggccagc gcaagtcggt cacgatcctg cgcgacttcg
     1321 cgaacatccg ctccaacaag cccaaggcga tggtgttcga cttcttcgcc aagcccgttt
     1381 ccatcgaggg cggcgaccgg gcggagcacc tagtcgtcga acgcaccgag cttgacgaga
     1441 aaggcggcgc ccgcggcacc ggcgagacct acgaagtccc cgcgtcgctg gtcgtcagct
     1501 gcatcggcta ttcgaccccg ccgatcgagg gcgttcccta tgacgatcgc ggcggcaagt
     1561 tcctcaacga gaccgggaag atcgccgacc ggctctactg cgtcggctgg gcccggcgcg
     1621 ggccgaccgg gaccatcggc accaaccggc ccgatggcta tgaagtcgcc gaggccgttg
     1681 ccgcggccat gccacccgga agcaccggcg accgcgctgg tgcggaaggt ctcaggcgcc
     1741 tctttactga ccgctcgatc aacgccaccg acttcaccga ctggcgcaag atcgaagccg
     1801 ccgaggaagc cgccgcccgt tccggcagcc cgcgagagaa gatggtccga gtcgagcagt
     1861 ggctgcagac gctcggccat tagcttgcgc tatgtcgagg tgcgacctat gaagcccgcc
     1921 accctgcacc ggacgctcct tttgcgtccg ggagcccggt cggggagtag ctcagcctgg
     1981 tagagcactg tcttcgggag gcaggggccg gaggttcgaa tcctctctcc cc
//
LOCUS       DASWNU010000078         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_496176, whole genome shotgun sequence.
ACCESSION   DASWNU010000078 DASWNU010000000
VERSION     DASWNU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496176"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VGB57_01545"
     CDS             complement(<1..747)
                     /locus_tag="VGB57_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0130058.1"
                     /translation="MSDLDTFRADTRDWLEANCPPEMREPMRDESDACWGGRNFEFKN
                     EAQKRWMEVMAERGWTVPDWPAEYGGGGLSPAETKILRQEMTRIGARNPLMSFGISML
                     GPALLKYGTEEQKKRFLPEIARGEIRWCQGYSEPNAGSDLASLQTRCEDKGDHWLVNG
                     QKVWTSYADKADWIFCLVRTDFEAPKHKGISFLLFDMATPGVSTKPILLISGYSPFCE
                     TFFDDVVVPKSQLVGELNKGWDVAKYLLGHE"
     gene            847..1269
                     /locus_tag="VGB57_01550"
     CDS             847..1269
                     /locus_tag="VGB57_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130059.1"
                     /translation="MKKRHVLFVIAAAAASSPAVAGGLEELVVREGLTVEQLEADGKI
                     CLKEAKNAERGRPVPSLPRTGNSAGDLAGAAAAGALKGLSDVKRFEYVHNACLSRLGY
                     RLVQMTPEERKAFSRTKGKAAGLAYIVEFSRRAIAEGR"
     gene            1291..1767
                     /locus_tag="VGB57_01555"
     CDS             1291..1767
                     /locus_tag="VGB57_01555"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0130060.1"
                     /translation="MRAALLAVAAAIPLAACAAATDRPQVEYFPPDESVQAAARAGGF
                     RLPFSSAVRVGDVLYLSGVIGTGSDGALAPGMEAQTRRTMENIGAMLARAGRSFDDVF
                     KCTVMIDDMKEWQAFNQVYMTYFKPDRLPARSAFGADGLALGALVEVECLAHAPAR"
     gene            complement(1805..3058)
                     /locus_tag="VGB57_01560"
     CDS             complement(1805..3058)
                     /locus_tag="VGB57_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130061.1"
                     /translation="MGQREGIFPTFFLSGFECSTFIWKDQGRRDLCEETQHLGHSDED
                     YRMLRSLGIGVAREGIPWPLVDRGGGRYDFSCIDPVLDAQRRHNILPIWDLCHYGYPD
                     GLDPFGPDFTPRFVAYARAAARYVADRAHHGPLCFTPMNEPTFFGYMAGEWAWAAPFG
                     RDRETRRRLTLACAAADIAAVKVIRSDFPDARMVHIDPLIWVVPPRDRPDLAEAAHLE
                     SHDDAYLAWDAISGRRFPELGGSPEILDIVGFNNYSFGQMEYREKGPHAALEPGDDRI
                     RPLCDLIEEGAKPYGRPCIVAETSGLKGGRPDWLNDVVAESLAALNRGVDLQGICLFP
                     AVDMPDWGTGEWVKNGIADLERLPNGTLMRVPYVPYVETLHRWQRRLNRVTELDEDPF
                     DKTVDLEDIVKAAKEMAPEADADWH"
     gene            complement(3102..3896)
                     /locus_tag="VGB57_01565"
     CDS             complement(3102..3896)
                     /locus_tag="VGB57_01565"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY0130062.1"
                     /translation="MSNLSPNFLLLTRVGFAARGLLYLIIGYLALKLGRTEDAGGALE
                     HVSREAGGLVLGLLALGFVAYGIWRVADAIFDTQNKGNDAKGIAGRLAGTLSGLIHLG
                     LAFTAVKLASGNGSGGDSSNSAESGAATAMGLPGGDLLLYLAAAVLAAAGIAQLAIAA
                     KRGFCKHLAAEAKNKAWVIAAGVGGHAARGAIFLSAGWLLFGAARHNEAEEAGALGDA
                     LLNLPAALQAAVAAGLCLFGIYSLIEARYRIIPDPHVKHRVASALT"
     gene            4017..5255
                     /locus_tag="VGB57_01570"
     CDS             4017..5255
                     /locus_tag="VGB57_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0130063.1"
                     /translation="MDFDLTERQAFYRDRVRDFVETRIRPRNKDYKIQLEAGDRWQPI
                     EVIEELKPEARAAGLWNLFMPPGQALRHVDESFEFEGTQLTNLEYALCAEEMGRIGWA
                     SEVFNCSAPDTGNMEVFHRYGTLEQKEQWLRPLMNGEIRSAFLMTEPAVASSDATNIQ
                     CEIRRDGDDYVLNGRKWWSSGAGDPRCKVAIVMGKTDTGADRHRQQSMVLMPLDAPGV
                     TVERALTVYGYDDAPHGHMEIELKDVRIPASNMLLGEGRGFEIAQGRLGPGRIHHCMR
                     TIGVAEEALKAMVKRLQTRTAFGKRLMEHSVWEQRVAEARIDIEMCRLLCLKAADMMD
                     KAGNKAAKLEIAMIKVQAPNMALRIIDDAIQAFGGAGVSEDAGLAKAYAHQSTLRLAD
                     GPDEVHNRAIARIEFSKHAV"
BASE COUNT          906 a         1777 c         1781 g          853 t
ORIGIN      
        1 ctcgtggccg aggagatatt tggcgacgtc ccagcccttg ttgagctcgc cgacgagttg
       61 cgatttcggc accaccacgt cgtcgaagaa ggtctcgcag aagggggagt aaccggagat
      121 gagcaggatc ggtttggtcg agacgccggg cgtcgccatg tcgaagagga ggaagctgat
      181 ccccttgtgc ttgggcgcct cgaaatcggt gcgcaccagg cagaaaatcc agtccgcctt
      241 gtcggcgtag ctggtccaga ccttctggcc gttcaccagc caatggtccc ccttgtcctc
      301 gcatctcgtc tggagcgagg cgaggtctga gccggcattg ggctcagaat agccctggca
      361 ccagcgtatc tcgccccgcg cgatttcggg gaggaagcgc ttcttctgct cctccgtccc
      421 gtatttcagc agcgccggcc caagcatcga gatgccgaag ctcatcagcg ggttgcgggc
      481 gccgatccgg gtcatctcct gcctgaggat cttggtctcc gccggcgaaa gcccgccgcc
      541 gccatattcg gccggccagt cgggcacggt ccagccgcgc tccgccatca cctccatcca
      601 gcgtttctgg gcctcgttct tgaactcgaa gttgcgcccg ccccagcagg cgtcgctctc
      661 gtcccgcatc ggctcgcgca tctcgggagg gcaattcgcc tccagccagt cgcgggtgtc
      721 ggcgcggaag gtgtccaggt cgctcacgga ggcctcctca gctttacgtt aacgtcaatg
      781 gcgcggagcg aggaaggcgt caaccgcaca tggaccggga cggtttgtgc ctctaggatc
      841 ggccacatga agaaacgtca tgtcctgttc gtcatcgccg ccgccgccgc ttcttcgccg
      901 gccgttgccg gcggcctcga ggagctcgtg gtccgcgagg gcctcaccgt cgaacagctg
      961 gaggcagacg gcaagatctg cctcaaggag gccaagaacg ccgaacgcgg tcggccggtc
     1021 ccgtcgctgc ccaggaccgg caatagtgcc ggcgacctgg ccggggcggc cgccgccggc
     1081 gcgctgaagg ggctgagcga cgtcaagcgc ttcgaatatg tccataatgc ctgcctcagc
     1141 cgcctgggtt atcgtctggt ccagatgaca cccgaggagc gcaaggcgtt cagccggacg
     1201 aagggcaagg ccgcgggcct cgcctacatc gtcgagttca gccgccgcgc catcgccgaa
     1261 gggcgctgac gcgctcgaca gggggacgga atgcgcgcag ccttgcttgc cgtcgccgcc
     1321 gcgataccgc tggccgcctg cgcggctgcc acggaccggc ctcaggtcga atactttccc
     1381 cccgacgagt cggtgcaggc ggcggcgcgg gcgggcggat tccgcctgcc tttctcgtcg
     1441 gcggtgcggg tcggcgacgt gctctacctg tccggggtga tcggcacggg cagcgacggc
     1501 gcgctcgctc cgggaatgga ggctcagacg cgccggacga tggagaatat cggagcgatg
     1561 ctcgcccgcg ccggccgctc cttcgacgat gtgttcaaat gcacggtgat gatcgacgac
     1621 atgaaggagt ggcaggcgtt caaccaggtg tacatgactt atttcaagcc ggaccgcctt
     1681 cccgcccgaa gcgccttcgg cgccgacggc cttgcgctgg gcgcgctcgt cgaggtcgag
     1741 tgcctggcgc acgcgccggc cagatgatca gtgcagattc gatgtccgac accgaatttc
     1801 cagttcagtg ccagtccgcg tccgcctccg gcgccatctc cttcgccgcc ttgacgatgt
     1861 cctcgaggtc cacggtcttg tcgaacggat cctcgtccag ctcggtcacc cggttcagcc
     1921 gtcgctgcca gcggtgcaga gtctcgacat aaggcacgta gggcacccgc atcagggtcc
     1981 cgttcggcag ccgttcgagg tcggcgatgc cgttcttgac ccattcgccg gtgccccagt
     2041 cgggcatgtc gacggccggg aacaggcaaa ttccctgcag gtccactcct cggttcagcg
     2101 ccgccaggct ctccgcgacg acgtcgttca gccagtctgg cctcccgccc tttagccccg
     2161 acgtctcggc gacgatgcag ggccggccgt agggcttcgc gccctcctcg atcaggtcgc
     2221 agagcggccg gatgcgatcg tcgcccggct cgagcgcggc atgcggcccc ttctcgcgat
     2281 attccatctg gccgaagctg tagttgttga agccgacgat atcgaggatc tccggcgaac
     2341 ctcccagctc cgggaagcgc cggccggaga tcgcgtccca ggcgagatag gcgtcgtcgt
     2401 gggattcgag gtgcgccgcc tcggcgaggt ccggccggtc gcgcggcggc accacccaga
     2461 tgagcgggtc gatgtggacc atccgcgcat ccggaaagtc ggagcggata accttgaccg
     2521 ccgcgatgtc ggccgcggca caagccaggg tcagccgccg cctcgtctcg cggtcccttc
     2581 cgaacggcgc cgcccaggcc cattcgccgg ccatgtagcc gaagaaggtc ggctcgttca
     2641 tcggcgtgaa gcagagcggc ccgtgatgcg cccggtcggc gacgtagcgc gcggcggccc
     2701 gggcataagc gacgaagcgc ggcgtgaaat ccggtccgaa ggggtccagg ccgtcgggat
     2761 atccgtaatg gcacaggtcc cagatcggca ggatgttgtg gcgccgctgg gcgtccagga
     2821 ccggatcgat acaggagaag tcgtagcggc cgccgccccg gtcgacgagg ggccagggta
     2881 tgccctcgcg cgccaccccg atgccgagcg agcgaagcat ccggtaatcc tcgtccgaat
     2941 gccccagatg ctgggtttcc tcgcaaaggt cgcggcggcc ctggtccttc cagatgaagg
     3001 tcgagcattc gaagccggac aggaagaagg tggggaaaat cccctcgcgc tggcccatgg
     3061 aaggcaagta cgcttgggcg accgtttcgg accccttggg attaggtcaa agcgctcgcg
     3121 acccgatgct tcacgtgggg atcggggatg atccggtagc gggcctcgat caggctgtag
     3181 atcccgaaca ggcacaggcc cgcggcgacg gcggcctgga gcgcggcggg caggttcaga
     3241 agggcgtcgc cgagagcgcc ggcctcctcg gcctcattgt gccgggcggc gccgaacagc
     3301 agccagccgg cggagaggaa gatcgcgccc cgcgcggcat ggccgccgac gcccgcggcg
     3361 atcacccatg ccttgttctt cgcctcggcc gccagatgct tgcagaagcc ccgcttcgcg
     3421 gcgatcgcga gctgggcgat cccggcggcg gcgagtaccg ccgcggccag atagaggagc
     3481 aggtcgccgc ccggcaggcc cattgcggtc gccgctccgc tttccgcgct gttgctcgaa
     3541 tcgccgcccg agccgttgcc ggaggcaagc ttcaccgcgg tgaaggcgag accgagatgg
     3601 atgagcccgc tgagcgttcc ggcgagccgc ccggcaatgc ccttggcatc gttgcccttg
     3661 ttctgcgtgt cgaaaatcgc gtcggccacc cgccagatgc cgtaagcgac gaagccgagc
     3721 gccagcaagc ccagcaccag ccctccggct tcacgcgaaa catgttccag ggcgccgccc
     3781 gcatcctcgg ttcgcccaag cttcagagcc agatatccga tgatgaggta gagcaggcct
     3841 cgagcggcga agccgacccg ggtcagcagg agaaagttgg gggacaggtt cgacatcccg
     3901 tcttaacgta cgttaacggg cgactgttcc gcaggcgaac ctatcagcac gccactggcg
     3961 cgaaaccgcg ctttctggca gcggggacca ctcagcagag agaggaatcc ggccggatgg
     4021 acttcgacct tacggaacgg caggcttttt accgcgaccg ggttcgcgat ttcgtcgaga
     4081 ctcggattcg gccgcggaac aaggactaca aaatccagct tgaggccggc gatcgctggc
     4141 aaccgatcga ggtgatcgag gagctcaagc ccgaggccag ggccgccggc ctctggaacc
     4201 tgttcatgcc ccccggccag gcgctccgcc atgtcgacga gagcttcgag ttcgagggca
     4261 cccagctcac caacctcgaa tatgcgctct gcgccgagga gatgggccgg atcggctggg
     4321 cctcggaagt gttcaactgc tccgcccccg ataccggcaa catggaggtc ttccaccgct
     4381 acggaacgct cgagcagaag gagcagtggc tccggccgct gatgaacggc gagatccgct
     4441 cggccttctt gatgaccgag cccgccgtgg cctcctccga cgccaccaac atccagtgcg
     4501 aaatccgccg cgacggcgac gactatgtgc tgaacggccg caaatggtgg tcgtcgggcg
     4561 cgggcgatcc gcgctgcaag gtcgccatcg tcatgggcaa gaccgacacc ggcgccgaca
     4621 ggcaccgcca gcagtcgatg gtcctgatgc ccctcgacgc gcccggggtt accgtcgagc
     4681 gcgcgctcac cgtctacggc tatgacgacg ccccccacgg ccacatggag atcgagctca
     4741 aggatgtgcg catccccgcc tccaacatgc tgctcggcga aggccgcggc ttcgagatcg
     4801 cgcaggggcg cctcgggccg ggccgcatcc atcattgcat gcgcaccatc ggggtcgccg
     4861 aggaggcctt gaaggcgatg gtgaagcgcc tccagacccg caccgccttc ggcaagcgcc
     4921 tgatggagca cagcgtctgg gagcagcgcg tcgccgaagc gcggatcgac atcgagatgt
     4981 gccggctgct ctgcctcaag gccgccgata tgatggacaa ggccggcaac aaggcggcca
     5041 agctcgagat cgcaatgatc aaggtccaag cgccgaacat ggcacttcgg atcatcgacg
     5101 atgccatcca ggcgtttggc ggcgccggtg tgtccgagga tgcggggctg gccaaggcct
     5161 acgcccatca gagcacgctg cggctcgccg acgggccgga cgaagtgcac aaccgggcga
     5221 tcgcccgcat agagttttcc aagcatgcgg tgtgagccgg aatttgcctc cctgccgcga
     5281 agcggtgggg agggggacca tgcgaagcat ggtggag
//
LOCUS       DASWNU010000079         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_496269, whole genome shotgun sequence.
ACCESSION   DASWNU010000079 DASWNU010000000
VERSION     DASWNU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496269"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGB57_01575"
     CDS             complement(<1..266)
                     /locus_tag="VGB57_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0130064.1"
                     /translation="MKLAIVDVGCGNIGSVGIALERFGIAPVVTGEAEEIAAADRVVL
                     PGVGAAGYAMEQIGARALAPVLRALRQPALGICLGMQLLFERSD"
     gene            complement(263..1921)
                     /gene="hisC"
                     /locus_tag="VGB57_01580"
     CDS             complement(263..1921)
                     /gene="hisC"
                     /locus_tag="VGB57_01580"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0130065.1"
                     /translation="MSSLASRLARPEILALPEFDIAANANDSFGSDAIKLDANENPYA
                     PLTGGALAAGLNRYPEPQPARLKQAMAALYGVSPENLVVTRGADDAIDILVRTFCRPG
                     KDAVSICTPTFSAYAHFARLQGARVLDTPLDAEFDFSPGSFIDAVGSEPNLKLAFICA
                     PNNPTGNPVPLAHIEAVCLALPNSIVVVDEAYIEFSEQKSAAERAIRSGNIVVLRTLS
                     KAYGLAGARVGCAIGHPETIALLTRALPPYPLPSLSIEAAMSALSPSRRIVHAERIAR
                     IKSDREGMAERLADSPLVRRVRSGGGNFLFLEVEDPDGLARKLGSLGIRLRFRPNAAP
                     GGVRLTIGTEAENLAALAAFKVAPEAVPGRRAELVRDTKETAIAVAVDLDRASPRRIE
                     TGIPFYDHMLDQVAAHGGFSLILSCEGDLDIDGHHSIEDCAIALGAALSRALGDRRGI
                     GRFGFSLPMDETEAHVLIDLSGRPYSVFDGGFEASHIGAYPTEMTRHVFRSLADSLGA
                     AIHVRVQGENDHHKTEACFKAFGRALRQALARDGDSLPSTKGVL"
     gene            complement(1918..>2419)
                     /locus_tag="VGB57_01585"
     CDS             complement(1918..>2419)
                     /locus_tag="VGB57_01585"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0130066.1"
                     /translation="QVLLVTPSLVLAESVRAELSAQVAALPRRGIAEASLAEARIILS
                     AGLPEAVEIANRYAPEHLSLAVADPEPLIAGIRNAGAVFAGYGAAETFGDYLAGSSHV
                     LPTDGAARAWSGVSVHTFLKAISVQTVTPEAAGRLAGPAAALARLEGLEAHARAADAR
                     LEEALP"
BASE COUNT          392 a          791 c          877 g          359 t
ORIGIN      
        1 tcatcgctcc gctcgaagag aagctgcatc cccaggcaaa tgccgagcgc aggctgccgg
       61 agggctcgaa gcaccggagc gagggcgcgc gcgccgatct gctccatcgc atagccggcg
      121 gctccgaccc cgggcaggac caccctgtcc gcggccgcga tctcctcggc ctcgccggtc
      181 acgaccggag cgattccgaa ccgctcgagc gcgatcccca ccgagccgat attgccgcag
      241 ccgacgtcga cgatggcgag cttcacagca cccccttggt gctcggcagc gaatcgccgt
      301 cacgcgccag cgcctggcgc aaggcgcggc cgaacgcctt gaagcaggct tcggtcttgt
      361 ggtggtcgtt ctcgccttgg acccggacgt ggatcgccgc tccgaggctg tccgcgaggc
      421 tccggaagac gtggcgggtc atctcggtcg gataggcgcc gatgtggctg gcctcgaacc
      481 cgccgtcgaa cacgctgtag gggcggcctg aaaggtcgat gagaacgtgc gcctcggtct
      541 cgtccatcgg cagcgagaag ccgaagcggc cgatgccgcg ccggtctccc agcgcgcgcg
      601 atagggcggc ccccagcgcg atggcgcaat cctcgatgct gtggtggccg tctatgtcga
      661 gatcgccctc gcaggagagg atgagcgaga agccgccatg cgccgccacc tggtcgagca
      721 tatgatcgta gaaggggatg ccggtctcga tccttcgcgg cgacgcccgg tcgagatcga
      781 cggcgaccgc gatcgccgtc tccttcgtgt cccggacgag ctccgcgcgc cggcccggca
      841 cggcctcggg cgccactttg aaggcggcga gtgcggcgag gttttccgct tcggtgccga
      901 tggtgaggcg cacgccgccc ggggcggcat tgggccggaa gcggagccgg attcccaggc
      961 ttccgagctt cctcgccagc ccgtccggat cctccacttc aaggaagaga aagttgccgc
     1021 cccccgagcg aacccgcctg accagcggcg agtcggccag ccgctcggcc atcccctcac
     1081 gatcggactt gatgcgggcg atccgctccg catggacgat ccggcgcgag ggggacagag
     1141 cggacatcgc ggcttctatg gacaggctcg gaagcgggta gggcggcaga gcgcgggtga
     1201 ggagagcgat cgtctccggg tggccgatcg cgcacccgac tcgagcgccg gcgaggccgt
     1261 aggccttgga gagagtgcgc aggacgacga tattgccgct ccggattgcg cgttccgcag
     1321 cgctcttctg ctctgaaaac tcgatatagg cctcgtccac cacgacgatc gagttcggca
     1381 aggcgaggca cacggcctcg atatgggcca gggggaccgg attgccggtc gggttgttgg
     1441 gggcgcagat gaaggcgagc ttcagattcg gctcggagcc cacagcgtcg atgaaggatc
     1501 cagggctgaa gtcgaactcc gcatcgagcg gcgtatcgag aacccgcgca ccctgcagtc
     1561 gggcgaaatg ggcgtaggcg gagaaggtcg gagtgcagat cgacaccgca tccttaccgg
     1621 gccggcagaa ggttcgaacg aggatgtcga tcgcatcgtc cgcgccgcgg gtgacgacga
     1681 ggttctcggg cgacacgccg tagagcgccg ccatggcctg tttcagacgg gcgggctgcg
     1741 gctccgggta gcggttcaac ccggccgcga gggcgcctcc ggtcagcggc gcgtaggggt
     1801 tttcgttggc atccagcttg atcgcgtcgc ttccgaacga atcgttggca ttggccgcga
     1861 tgtcgaactc gggaagggca aggatttcgg gccgggcgag gcggctggcc agcgagctca
     1921 tggcagcgcc tcctccagtc gggcatcggc ggcgcgggcg tgcgcctcca gtccttccag
     1981 gcgcgccagg gcggccgcgg ggccggcgag acgaccggca gcctcgggcg tgacggtctg
     2041 gacgctgatc gccttgagga aggtgtggac cgacactccg ctccaggcgc gggcggcgcc
     2101 gtcggtgggg aggacgtggc tggacccggc cagataatcg ccgaacgtct cggccgctcc
     2161 atatcccgcg aagactgcgc cggcattgcg tatcccggcg atcagcggtt ccggatccgc
     2221 caccgcaagc gagagatgct ccggcgcgta gcgattggcg atttcgacgg cttcgggaag
     2281 gccggcggac aggatgatcc gggcctcggc gagcgaggct tcggcaatcc cgcggcgggg
     2341 aagtgccgcg acttgcgccg acaactccgc ccgcaccgat tcggccagga caagggaggg
     2401 cgtgacgagc aggacctgc
//
LOCUS       DASWNU010000080         7022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_418660, whole genome shotgun sequence.
ACCESSION   DASWNU010000080 DASWNU010000000
VERSION     DASWNU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418660"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2338)
                     /locus_tag="VGB57_01590"
     CDS             complement(<1..2338)
                     /locus_tag="VGB57_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0130067.1"
                     /translation="MWRHYVTVGFRAFTKNRTNAAINIFGLAVGLAACLLLLIYVRYE
                     TSYDEWLPGAEDAYQLQILGNDPDTGEDRSLQQTQYVAGTAVAKDFPQIQAKVFALKM
                     GVTAIKDGQPLTLPEGLAVDGPLFDVLPLPLARGDPATALAAPGNVVIAEKEAAKLFG
                     GADPLGKTLTLVEGGQRVDYRITGVLKDIPRNSHLRASLIQRFDPAVDFASFPGFMTS
                     WGQISGWNYVRLKPGSRAADVHSAMPAWERRNIPPPVQEGEPDMGKLGDWRLANVRDV
                     HLGKVGQGGMTPSNDARTITTFFVIAMLILGMAIVNFTNLATARASQRAREVALRKVL
                     GAGRRQLVMQFMGESLLVTAAAMLLALAAVELLLPVLSAYLDADLAMAYLGADGMLLP
                     VIGLTLLVGTAGGLYPALYLSRYQPGFVLKANKSSAEPRGAGRLRTVLVVAQFAVSIG
                     LIACTAIVYAQTVYARTADPGFKRDGILQISGINGGPVVPLQEAMIRQIAAVEGVVSV
                     GRTGIGVDTPNNMGRNVFLPGRKAPVIIGNYPVDENFFKTMGIDLVAGRGFDPARPAD
                     RFDAPFVNVDPAQQQALIDRGSNVVVNEYAARQLGFREPAQAIGKQVRIAQFMEPGAG
                     LVPATIVGVVRDTRFRSMHRSIDSIVYRLGNDVLTHMVVRYDPSRPPAQVRDRIGQVW
                     RRLVPDVPFKADFSEDIVRELYEAEQARAEMFAGFALLAIVIAALGLYGLAAFTAERR
                     TKEIGIRKVFGARVRDIVRLLAWQFSKPVILANLIAWPV"
     gene            complement(2349..4856)
                     /locus_tag="VGB57_01595"
     CDS             complement(2349..4856)
                     /locus_tag="VGB57_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0130068.1"
                     /translation="MWRNYLTVGLRALAKNKTYAFINIFGLAIGMAACLMILLYVRYE
                     ASYDAWMPGAERAYQFQDFYKATDNGGEEMALQMTSYVSGKALVKDFPQIEKAVYVAS
                     SGAVIMQNGQPSLADDFNFVDGNLFDVLQVPFVRGDRATALTNPNSLVLTETEASKRF
                     PGQDALGKTLTLIVQGKSADYVVGGVMKDLPKNSHLELGVIARFHPETFFAEQPSMLT
                     SWGNQGGWWYVKLREGAKAEDINRQLPAWEKRNIPDDTGGREKTNPGDDQDWKLTNVR
                     DIHLGEAQQASMTPGNDQRTIVTFAIVALLILGMAVVNFTNLATARASQRAREVALRK
                     VLGASRKQLITQFLGESMLVTVLAMLLALAMVELLLPLFNRFLDADIGLVYWGSGGVL
                     LPIVVLVLLVGAAGGLYPAFYLSRFQPARVLKANKSSAEAQGSGSLRNVLVVAQFAVS
                     IGLIICTAVIYAQTVYARTADAGYNRHGLLQLQGLAARQAQPVGETLRNEIAKLDGIK
                     GASLTGIGVSPGNNSVSSVYLPGSPKAIDLGTYVIDHSFIPTMGMKMLAGRNFSDTIA
                     MDDSTTPFPMDRAAEQAFARRGTNIVVSEAAAQRLGFRDPQQAIGKSISAGMTLPEFG
                     MVNANIVGVFKDARFRSVRDPLQPIMFLFARNGFNTMVIRYEGRDPKQVQADIERIWK
                     RLLPDLPFRAELADDRVARLYERDEARGQLFGAFALLAVVIGCLGLFGLAAFTAERRT
                     KEIGIRKVLGARSRDIVRLLAWQFSKPVIIANLIAWPAAWWVMRDWLNGFDSRIALGP
                     GPFVLAGGLALAIALGTIASHAIRVARANPIQALRYE"
     gene            complement(4867..>7022)
                     /locus_tag="VGB57_01600"
     CDS             complement(4867..>7022)
                     /locus_tag="VGB57_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0130069.1"
                     /translation="MFLDAAAVDPSFFDIFRIEFVDGSAQRALADMRSVVLTESEAVR
                     LVGTRMAVGRTITVGAETGRQDYPITGVIRDLPSNSSLKLAFIYRHNPADFDSLPAAD
                     KGWGNMDQQHYVKLRPGADAAAINAAFPAWEKRTIAPQLIDGRSSSQADILDLKLVPI
                     TGVHLGDAVQGALTPTGDSRTLATFGIVALLILGMAVMNFINLSTARATQRAREIALR
                     KVLGARRHQLVGQLLGESLLTAGIAMLLALALVEVGSAWSGGWIGADLKVRYLGEGGL
                     LLPALGLFLFAGLLGGLYPAFYLSRFQPSEVLRANKASAEAPGSGRLRATLVVLQFAI
                     AIGLIVCTSVIYWQTRHVQEVDPGFRRDGLIQIKSAWRFAGDSARYEAARRDLLAIPG
                     VVAAGRTNLGIAATNKSIQAVRAPGAAERLSMGFYAVDPDFFRAMDLRLLAGRFLGDS
                     FAEDKVVRTDGDAPAQVVGRGLNIVVNREAAKLMGFAEPQAAVGKSVRVGLDGSDDLT
                     PSTIVGVVGDTRIRTARDAIEPLLYRYDPQRTGQVLVRYAEARPRDVMDGLARVWRKF
                     EPEIPFQGAFAEDVIAELYAAERARGALFAAFSGLAVLIACLGLYSLATFATERRTKE
                     IGIRKVLGAKVRDIVRLLAWQFSKPVILANLVAWPVAWWAMRDWLNTFDARIALTPGP
                     FLLAGLLALAIAIGTVGGHALRVARLNPIHALRYE"
BASE COUNT         1185 a         2274 c         2443 g         1120 t
ORIGIN      
        1 caacgggcca tgcaatgagg ttggcgagga tcacaggctt ggagaattgc caggcgagca
       61 gccgcacgat gtcgcgcacc cgggcgccga acaccttcct tatcccgatc tccttcgtcc
      121 ggcgctcggc ggtgaacgcg gcgaggccgt acaggcccag ggcggcgatg acgatggcga
      181 gcagggcgaa gccggcgaac atctcggccc gagcctgctc cgcctcgtag agctcgcgga
      241 cgatatcctc gctgaaatcc gccttgaacg gcacgtccgg gacgagccgc ctccagacct
      301 ggccgatccg gtcgcgcacc tgcgcgggcg gccgggacgg gtcgtagcgc accaccatgt
      361 gggtgagaac gtcgttgccc agccggtaga cgatcgaatc gatgcttcga tgcatcgacc
      421 ggaagcgtgt gtccctgacc actccgacga tcgtcgccgg gacgagaccc gcccccggct
      481 ccatgaattg cgcaatccgc acctgcttgc cgatcgcctg ggcaggctcg cggaacccga
      541 gctgacgcgc cgcatattcg ttgacgacga cgttggagcc gcggtcgatc agcgcctgct
      601 gctgcgccgg atcgacattg acaaaaggcg catcgaagcg gtcggcgggc ctcgccggat
      661 cgaagccccg gccggcgacg aggtcgatcc ccatcgtctt gaagaaattc tcgtcgaccg
      721 ggtaattgcc gatgatcacc ggcgccttgc ggccgggaag gaagacgttc ctccccatgt
      781 tgttcggcgt atcgactccg attccggtcc ggccgacgga gaccacgccc tccaccgcgg
      841 cgatctgccg gatcatcgcc tcctggagcg gaacgaccgg gccgccgttg atgccggata
      901 tctggaggat tccgtcgcgc ttgaagccgg ggtccgcggt gcgcgcgtag accgtctgcg
      961 catagacgat ggccgtgcag gcgatgagcc cgatggacac cgcgaactgc gccaccacca
     1021 ggacggtgcg aagccgcccc gccccccgcg gctcggccga ggacttgttg gccttgagca
     1081 cgaagccagg ctggtaacgc gacagataca aagcggggta gagcccaccc gccgttccga
     1141 ccagcagggt cagtccgatc accggcagga gcattccgtc ggcgccgaga taggccatgg
     1201 caagatcggc atcgagatag gccgacagca ccggcagcag cagttcgacc gcggcgaggg
     1261 cgagcagcat ggccgccgcg gtcaccagca gggattcgcc catgaactgc atgaccaact
     1321 ggcgccggcc cgcgccgagc accttgcgaa gcgccacttc ccgtgcgcgc tggctggcgc
     1381 gggcggtcgc caggttcgtg aagttgacga tcgccattcc gaggatcagc atcgcgatga
     1441 cgaagaaggt cgtgatcgtt cgggcgtcgt tcgagggagt catcccgcct tggccgacct
     1501 tgccgagatg gacgtcgcgg acgttcgcga ggcgccagtc gccgagcttg cccatgtccg
     1561 gctcgccctc ctgcaccggc ggcgggatgt ttctcctctc ccaggccggc atggccgaat
     1621 gaacgtccgc ggcgcggctt ccgggcttca ggcggacata gttccagccc gatatctgtc
     1681 cccaggaagt catgaagccg gggaagctcg cgaaatcgac cgccgggtcg aagcgctgga
     1741 tgaggctggc gcgcaggtgg gagttgcgcg ggatgtcctt gaggactccg gtgatccggt
     1801 aatcgacccg ctggccgccc tcgaccaggg tcagggtctt ccccagcgga tccgcgccgc
     1861 cgaacagctt ggccgcctcc ttttcggcga tgacgacatt gccgggcgcg gccagagccg
     1921 tcgccggatc gccgcgcgcg agcggcagtg ggaggacgtc gaagagcggg ccgtcgacgg
     1981 cgagtccctc cggcagggtc agcggctgcc cgtccttgat cgccgtaact cccatcttca
     2041 gcgcgaagac cttcgcctgg atctgcggga agtccttggc gaccgcggtc ccggcgacat
     2101 attgggtctg ctgcaggctc cggtcttccc ccgtgtccgg gtcgttgccg aggatctgca
     2161 gctggtaggc gtcctcggcg cccggcagcc attcgtcgta gctcgtctcg taacggacgt
     2221 agatcaggag caggaggcag gcggccagcc cgacggcgag gccgaagatg ttgattgcgg
     2281 cattggtccg gttcttggtg aaggcccgaa agccgaccgt cacgtaatga cgccacatgg
     2341 tctcgttcct actcgtagcg gagggcctgg atcgggttcg cccgcgccac ccggatggcg
     2401 tggctggcga tggtgccgag agcgatggcc aaagcgaggc cgccggcgag gacgaacggc
     2461 cccggcccga gcgcgatccg gctgtcgaag ccgttcagcc agtcgcgcat cacccaccag
     2521 gccgccggcc aggcgatgag gttggcgatg atgacgggct tcgagaattg ccacgcgagc
     2581 agcctgacga tgtccctcga gcgggcgccc agcactttcc ggatcccgat ctccttggtc
     2641 cgccgctcgg cggtgaaggc ggcgaggccg aacaggccga ggcagccgat caccaccgcc
     2701 agcaaagcga aggcgccgaa cagctgcccc cgcgcctcgt cgcgctcgta gaggcgcgcg
     2761 acccggtcgt cggcgagctc ggcccggaag ggcagatcgg ggagaagcct tttccagatc
     2821 cgctcgatgt ccgcctggac ctgcttggga tccctgccct cgtagcggat caccatcgtg
     2881 ttgaagccgt tgcgagcgaa caggaacatg atcggctgga gcggatcgcg gacggagcgg
     2941 aagcgggcgt ccttgaaaac cccgacgata ttcgcattga ccattccgaa ttcgggcagc
     3001 gtcatgccgg cgctgatcga cttgccgatc gcctgctgcg ggtcgcggaa gccgagccgc
     3061 tgggccgccg cctcgctgac cacgatgttg gtcccgcgcc tggcgaaggc ctgctccgcc
     3121 gcacggtcca tcgggaaggg cgtcgtcgaa tcgtccattg cgatcgtgtc ggagaagttg
     3181 cggcccgcga gcatcttcat ccccatggtc gggatgaaag aatggtcgat gacgtaggtg
     3241 ccgaggtcga tcgccttggg gctgcccggc agatagaccg agctcaccga attgttgccg
     3301 ggcgacactc cgatcccggt cagcgaagcg cccttgattc cgtcgagctt ggcgatctcg
     3361 ttccgcaggg tctctccgac cggctgcgcc tggcgggcgg ccagcccctg cagctgaagc
     3421 agtccgtggc ggttgtagcc ggcatccgcc gtccgggcgt agacggtctg ggcgtagatc
     3481 accgccgtgc agatgatgag tccgatcgag accgcgaact gggcgacgac gaggacgttg
     3541 cggaggctcc ccgagccctg cgcctcggcc gacgatttgt tggccttgag cacccgggcc
     3601 ggctggaagc gcgacagata gaaagccggg tagagcccgc cggcggctcc aacgagcagc
     3661 accaggacga cgatcggaag gagcactccg cccgagcccc agtagacgag gccgatatcg
     3721 gcatcgagga agcggttgaa gagcggcagg agcagctcga ccatggccag ggcgagcagc
     3781 atcgccagca cggtcacaag catcgattcc ccgaggaact gggtgatgag ctgcttccgg
     3841 ctggctccga gcaccttgcg cagagccact tcccgcgccc gctgcgacgc ccgggcggtg
     3901 gcgaggttgg tgaagttcac caccgccatt ccgaggatga gcaaagcgac gatggcgaag
     3961 gtgacgatgg tgcgctggtc gttgcccggg gtcatcgacg cctgctgggc ctcgccgagg
     4021 tggatgtcgc ggacgttggt gagcttccaa tcctggtcgt cgccgggatt ggtcttctcg
     4081 cgcccgccgg tatcgtcggg gatgttgcgc ttctcccagg cgggaagctg ccggttgatg
     4141 tcctccgcct tggcgccctc gcggagcttg acgtaccacc agccgccctg attgccccaa
     4201 ctggtgagca tggagggctg ctctgcgaag aaggtctccg ggtggaagcg ggcgatcacc
     4261 ccaagctcga gatggctgtt cttcggcagg tccttcatca ccccgccgac gacatagtcg
     4321 gcgctcttgc cctggacgat cagggtcagg gtcttgccga gcgcgtcctg gcccggaaag
     4381 cgcttgctcg cctccgtctc ggtcaggacc agcgaattgg ggttggtgag agcggtggcg
     4441 cggtcgcctc ggacgaacgg cacctggagg acgtcgaaga ggttgccgtc gacgaagttg
     4501 aagtcgtcgg cgaggctggg ctggccgttc tgcatgatga cggcgcccga ggaggcgacg
     4561 tagaccgcct tctcgatctg cgggaaatcc ttcaccagcg ccttgcccga gacgtagctc
     4621 gtcatctgca gggccatctc ctccccgccg ttgtcggtcg ccttgtagaa atcctggaac
     4681 tggtaggcgc gctccgcgcc cggcatccac gcgtcgtagc tcgcctcgta gcgcacgtag
     4741 agcaggatca tcaggcaggc cgccattccg atggcgaggc cgaatatgtt gatgaaggcg
     4801 taggtcttgt tcttcgccag ggcccggaga ccgaccgtca ggtaattgcg ccacatggtt
     4861 cgggtcctat tcgtaacgga gggcgtggat tgggttcagg cgggcgaccc gcagggcgtg
     4921 gccgccgacg gttccgatag cgatggcgag cgcgagcaga ccggcgagga ggaacggccc
     4981 gggggtgagc gcgatgcgcg cgtcgaacgt gttcagccag tcccgcatcg cccaccaggc
     5041 gaccggccag gcgacgaggt tggcaaggat gacgggcttg gagaattgcc aggcgagcag
     5101 ccgaacgatg tcgcgcacct tggcgccgag caccttgcgg atcccgatct ccttggtgcg
     5161 ccgctcggtg gcgaaggtgg cgaggctgta gaggccgagg caggcgatca ggacggcgag
     5221 accggagaaa gcggcgaaca gggcaccccg cgcccgctcg gccgcgtaga gctcggcgat
     5281 cacatcctcc gcgaacgcgc cctggaaggg gatttcaggc tcgaacttgc gccagaccct
     5341 ggcgagtccg tccatgacgt cgcggggacg ggcttcggcg tagcggacga gcacctggcc
     5401 cgtgcgctgg gggtcgtagc gatagagcag cggctcgatc gcgtcgcggg cggtgcggat
     5461 cctcgtatcg ccgaccactc cgacgatggt cgaaggcgtg agatcgtcgc ttccgtccag
     5521 tccgaccctg accgacttgc cgaccgccgc ctgcggctcc gcgaagccca tcagcttcgc
     5581 cgcctcgcgg ttgacgacga tgttgagccc gcgtccgacc acctgtgccg gcgcgtcccc
     5641 gtccgtccgg acaaccttat cctcggcgaa gctgtcgccg aggaagcggc cggcgaggag
     5701 gcgaaggtcc atcgcccgga agaaatcggg atcgacggcg tagaagccca tcgacaggcg
     5761 ttccgcagcg cccggagccc ggaccgcctg gatgctcttg ttggtcgcgg cgattccgag
     5821 attggtcctc ccggcggcga cgactccggg tatcgcgagg agatcgcgac gcgcggcttc
     5881 atatcgggcg ctgtcgccgg cgaagcgcca ggccgatttg atctggatca ggccgtcgcg
     5941 gcggaagccc ggatccacct cctgcacatg gcgggtctgc cagtagatga ccgaggtgca
     6001 gacgatcagc ccgatggcga tggcgaactg gagcacgacc agggtcgccc gaagacggcc
     6061 gctgccaggc gcttcggcgg aggccttgtt cgcgcgaagc acctcggaag gctggaagcg
     6121 gctgaggtag aaagcggggt aaaggccgcc gagcaggccg gcgaagagga acaggcccaa
     6181 tgcagggaga agcaatccgc cttccccgag atagcggacc ttgagatcgg caccgatcca
     6241 gccgccgctc caggcgcttc cgacttcaac cagcgccagt gcgagcagca tcgcgatccc
     6301 ggccgtaagg agcgattccc ccagcagctg gccgacgagc tggtggcgcc gggctccgag
     6361 caccttgcga agcgcgatct cgcgggcacg ctgggtcgcc cgagcggtcg aaaggttgat
     6421 gaagttcatc accgccattc cgaggatgag caaggcgacg atcccgaagg ttgcgagggt
     6481 gcgcgaatcg cccgtcgggg tgagtgcgcc ctgcacggcg tcgccgagat ggactccggt
     6541 gatcggcacg agcttgagat cgaggatatc ggcctggctc gacgaccggc cgtcgatcag
     6601 ctgcggagcg atcgtccgct tctcccaggc cgggaatgcg gcgttgatcg ccgccgcgtc
     6661 cgcacccggc cgaagcttga cgtaatgctg ctggtccatg ttgccccagc ccttgtcggc
     6721 ggcggggagc gagtcgaagt cggcggggtt gtggcggtag atgaaggcca gcttcaggct
     6781 cgaattgctc ggcaggtccc ggatcacgcc cgtgatcgga tagtcctggc gcccggtttc
     6841 ggcgccgacg gtgatggtcc gccccacggc catgcgcgtg cccaccagcc tcacggcctc
     6901 gctttcggtg aggacgaccg agcgcatgtc cgccagggcg cgctgcgccg atccgtcgac
     6961 gaactcgatc ctgaagatgt cgaagaagct cggatcgacc gccgcggcgt cgaggaacat
     7021 cg
//
LOCUS       DASWNU010000081         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_237283, whole genome shotgun sequence.
ACCESSION   DASWNU010000081 DASWNU010000000
VERSION     DASWNU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237283"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VGB57_01605"
     CDS             <1..1242
                     /locus_tag="VGB57_01605"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEY0130070.1"
                     /translation="GKPVTAIAHDFTLQPGVPLMRVDNGACRGGKTAVTLRQGEFSRD
                     RPEAAARAWRVPVIASAVGGGEVRTLVAGGAGTLSVPGCNPVVVNSGQTGYYRTLYAP
                     ALLDRLTASFARLKPIDQIGLLADNWGLGLAGYQSAAEALDMVDAVPANANPQVWSRA
                     AAILSSVHNMYENDPAHRAMLERYAAAKLSPVLARIGWAPKAGEAPTVPVLRADLIET
                     LGEMGDARVVGEANRRLAAGDPLATGGPLRSTILAVTARNVDSAGWERLRAQARAERS
                     PLVKAQLYKLLGTARDPALAQRALDLALTDEPGATISAGLVSEVAEEHPDLAYDFAIR
                     NREKVEALVDISSRSRFLAGLGGGSADPAMVAKLEDFATRYMTPQSRGRVDVAIAAIR
                     DRVRVRTTRLPDITRWLEAKG"
     gene            complement(1377..>2856)
                     /locus_tag="VGB57_01610"
     CDS             complement(1377..>2856)
                     /locus_tag="VGB57_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY0130071.1"
                     /translation="AKGLSVLVWSSLVSAGFAILTQTRLVAAEASAWFRAGAGATGIS
                     GGLSFALMGVGHLVGISVGMAMFLGLAIGWWVLLPILTAQMPLEGGPQVWAGTIFTQH
                     VRFFGAGVIGVAAVWTLLKIARPVLGGIRSAMAASAARRGGQALALEERDLPIAIVGF
                     GSLAILAPIAWLLWTVIADGPLAGSAGLLIAGALVFILVIGLMIAAVCGYMAGLIGAS
                     NSPVSGIGILSVVAASLMLVGLFGRDTGPGTSEALVAYALIVTGLVFGIATISNDNLQ
                     DLKTGQLVGATPWRQQVALIFGVIFGSLVVPPVLQVLYEAFGFAGMPGAGPQALPAPQ
                     AALISALAKGVLGGDLNWTMIGYGALTGVGLVLLDEILGRAKTLRLPPLAVGIGIYLP
                     MAAVLPVVIGAVIGFFYDRWADRRAKPEFARRMGVLTATGLIVGESLWGVVFAGIVAG
                     SGRDSPLAVVGEGFESLALIGGTALFILLAAWVYRRTMRLAA"
BASE COUNT          495 a         1018 c          994 g          349 t
ORIGIN      
        1 ggcaagccgg tcaccgccat cgcccacgat ttcacccttc agcccggcgt gcccctgatg
       61 cgggtcgaca acggggcctg ccgcggcggc aagaccgccg tgaccctgcg ccagggggag
      121 ttcagccgcg accggccgga agcggcggcc agggcctggc gggtgccggt gatcgcctcg
      181 gccgtcggcg gaggcgaggt acggaccctg gtggccggcg gcgccggtac cctcagcgtc
      241 cccggctgca acccggttgt cgtcaacagc ggccagaccg gctattaccg cacgctctac
      301 gcgccggctc tgctcgaccg gctcaccgca agcttcgccc ggcttaagcc gatcgaccag
      361 atcggactgc tcgccgacaa ttggggcctc ggcctcgccg gctatcagtc ggccgccgag
      421 gcgctcgaca tggtggacgc ggtgcccgcc aacgccaatc cgcaggtgtg gagccgggcc
      481 gccgcgatcc tgagcagcgt ccacaacatg tacgagaacg accccgccca ccgggcgatg
      541 ctcgagcgct acgccgccgc gaagctgtcg ccggtgctcg cccggatcgg ctgggctccc
      601 aaggccggcg aggcgccgac cgttccggtg ctccgggcgg acctgatcga gactctcggc
      661 gagatgggcg acgccagggt ggtcggcgag gccaaccgga ggctcgcggc cggcgatccg
      721 ctggcgacgg gggggccgct ccgaagcacg atcctcgccg tcaccgcccg caacgtcgat
      781 tccgccggct gggagcgcct cagggcacag gcgcgcgcgg agcgcagccc gctggtcaag
      841 gcccagctct acaagctgct cggcaccgcc cgcgacccgg cactggcgca aagggcactc
      901 gacctcgccc tcaccgatga gccgggcgcc acgatcagcg ccggcctcgt ctcggaggtg
      961 gccgaggaac atcccgatct cgcctacgat ttcgccatcc gcaaccgcga gaaggtcgag
     1021 gcgctggtcg acatctcgtc gcgctcgcgc ttcctcgccg gactcggcgg aggctcggcc
     1081 gaccccgcca tggtcgccaa gctcgaggat ttcgcgaccc gctacatgac tccgcagtcg
     1141 cgaggacgcg tcgacgtggc catcgccgcg atccgcgacc gggtgagagt gcgcaccacg
     1201 aggctgccgg acatcactcg gtggctggag gcaaagggct gataaatcct ccctgtcgcg
     1261 aagcggtggg gagggggacc acccgaaggg tggtggaggg gtaactgacg aggcttctgc
     1321 cagctacccc tccgccatgc ttcgcatggt cccctcccca tcttcgatgg ggaggatcaa
     1381 gcggccagcc gcatcgtcct gcggtaaacc cacgccgcca gaaggatgaa cagggccgtc
     1441 ccgccaatca gcgcaagaga ctcgaacccc tccccgacga cggccaaagg cgaatctctc
     1501 cccgacccgg cgacgatgcc cgcgaagacg actccccaaa ggctctcgcc gacgatgagg
     1561 ccggtggcgg tcagcacgcc catgcgccgg gcgaattcgg gtttcgcccg gcggtccgcc
     1621 cagcgatcgt agaagaagcc gatcaccgct ccgatcacga ccggcaggac ggccgccatc
     1681 ggcaggtaga ttccgattcc gacggcgagc ggcggcaggc gcagcgtctt ggcccggccg
     1741 agaatctcgt cgaggaggac cagccccacg ccggtgagcg cgccgtagcc gatcatcgtc
     1801 cagttgaggt cgccgccgag cacgcccttg gcgagcgcgg agatgagcgc ggcctgcggc
     1861 gcaggcaagg cctgcggccc ggcgcccggc attccggcga agccgaaggc ctcgtagagg
     1921 acctgaagga cgggcggcac caccagcgag ccgaagatga ctccgaagat cagggcgacc
     1981 tgctggcgcc agggcgtggc tccgaccaat tgaccggtct tgaggtcctg gaggttgtcg
     2041 ttcgagatgg tggcgatccc gaagacgagg ccggtgacga tcagcgcgta ggcgaccaat
     2101 gcctcggaag tgcctggccc ggtgtcgcgg ccgaacaggc ccaccagcat cagcgaggcc
     2161 gcgaccacgg aaaggattcc gatgccggag acggggctgt tcgaggcgcc gatcaggccg
     2221 gccatgtagc cgcagacggc ggcgatcatc aggccgatga cgaggatgaa gacgagcgct
     2281 cccgcgatca gaagcccggc cgagcccgcg agcgggccgt cggcgatcac cgtccacaag
     2341 agccaggcga tcggcgccag gatcgcgagc gagccgaagc cgacgatcgc gatcggaagg
     2401 tcgcgctcct ccagcgcgag cgcctggccg ccgcgccggg cggcgctcgc cgccatcgcc
     2461 gagcggattc cgccgagcac cggccgggcg atcttgagca gggtccagac cgcggcgacc
     2521 ccgatcactc cggcgccgaa gaagcgaacg tgctgggtga agatggtgcc cgcccagact
     2581 tgcggaccgc cctcgagcgg catctgcgcc gtcaggatcg gaagcaggac ccaccagccg
     2641 atcgccaggc cgaggaacat cgccatcccg accgagattc cgacgaggtg gccgacgccc
     2701 atcaaagcga atgaaaggcc tcccgaaatc ccggtcgcgc ccgcgcccgc ccggaaccag
     2761 gccgaggctt cagcggcgac cagccgggtc tgggtgagga tggcgaagcc cgccgagacg
     2821 aggctgctcc agacgaggac ggaaaggcct ttggcg
//
LOCUS       DASWNU010000082         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_262558, whole genome shotgun sequence.
ACCESSION   DASWNU010000082 DASWNU010000000
VERSION     DASWNU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262558"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VGB57_01615"
     CDS             complement(<1..1200)
                     /locus_tag="VGB57_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0130072.1"
                     /translation="MRGLFERFGVTWPTSGDWIVAAIAVALVALAWGLAWLASRWLGP
                     RVAAFWEHRAGGRGEALARRMCDLTRYLTGALVVAIALNADAWRPLGAMVLGAALAAW
                     AALLVSGLVRGLQMPRWIAALLAGVTFVSLLARAVGGLKPITDTLDRVGFTAGQTRVS
                     LLTVLQVLVTLVAIYAVVKLLNRIVGHSIRRATGLDATQQLLGQKLASVAILIAAFFI
                     GIDLIGIDLTALAVFSGAFGLAVGFGLQKTFGNLIAGIILLMDRSIKPGDVIVVGDSF
                     GHVNKIGVRAVSIVTRDGKEHLIPNENLMTQEVENWSYSSRDVRVHIPVGIAYDCDLP
                     LAQKLMIQAAGEAGRVMQNRPPKVWLIAFGESSVDHEILVWISDPEQGVGSVRSEILN
                     RVWTLFKE"
     gene            complement(1197..2414)
                     /gene="metC"
                     /locus_tag="VGB57_01620"
     CDS             complement(1197..2414)
                     /gene="metC"
                     /locus_tag="VGB57_01620"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY0130073.1"
                     /translation="MGHGSRGSQGDGAGVKDGRPDTKLVHGGRREEWRGRTVNPPVHR
                     ASTILFDTVAEMRAAAPAFGEYYYGLHGTPTHWALSEALTELEPGAAGTMLYPNGLAA
                     VTAALLSVLSAGDELLMTDSVYGPTRRFCDVFLKRYGVATRYYDPLVGAGIADLIGER
                     TKAVFLESPGSLTMEVQDVPAICAAAKAKGVATLLDNTWATPLFFPALAAGVDLTILA
                     GTKYVGGHADVMLGSVTAGARWYPKLERTSWDLGQSVSPDDAWLCSRGLRTMGVRLRR
                     HEASALRVARWLKEQPQVAAVLHPALPDCPGHEEWTRDFRGSSGLFTFVLNGGDEAER
                     TRFVEALDLFGLGYSWGGYESLAVPVDPERLRTVTRWRAEGPLVRLHIGLEDPDDLIE
                     DLAAALAGYGASE"
     gene            complement(2369..>3005)
                     /locus_tag="VGB57_01625"
     CDS             complement(2369..>3005)
                     /locus_tag="VGB57_01625"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY0130074.1"
                     /translation="PKFASRMASLGLRDGQRFVVYDNSPLHSAARAWWMLKSFGAHYV
                     AILDGGLQKWKAEGRPLEAGRPQVRHGHFSPSFEAGAVADKATVAGLLGDGRHEIVDA
                     RGPGRFTGEEAEPRPGMASGHIPGSKNLPQGRLFSDDNSWKQGDELRSAFESAGVDLG
                     KPMVATCGSGITACVLLFGAHLLGKEDVRLYDGSWSEWGMDPAAPKATGPA"
BASE COUNT          493 a         1054 c         1029 g          429 t
ORIGIN      
        1 ctccttgaac agcgtccaga cccggttgag tatctccgac cggacgctgc cgacgccctg
       61 ttcgggatcg ctgatccaga ccagtatctc atggtcgacg ctgctctccc cgaaggcgat
      121 cagccacacc ttgggcggcc ggttctgcat cacccttccc gcctcgcccg ccgcctggat
      181 cattagcttc tgggcgagcg gaaggtcgca gtcgtaggcg attccgaccg ggatatgcac
      241 ccgaacgtcg cggctcgaat aggaccagtt ctccacctcc tgggtcatca gattctcgtt
      301 ggggatcaga tgctccttgc cgtcccgggt gacgatcgag accgccctca ccccgatctt
      361 gttgacgtgg ccgaagctgt cgccgacgac gatgacgtcg cccggcttga tcgacctgtc
      421 catcagcagg atgatcccgg cgatgaggtt gccgaacgtc ttctggagcc cgaagccgac
      481 ggcaagcccg aacgcgcccg agaagacggc gagcgcggta aggtcgatcc cgatcaggtc
      541 gatcccgatg aagaaggcgg cgatcaggat ggcgacgctg gccagcttct ggccgagcaa
      601 ttgctgggtc gcatcgaggc cggtggcgcg ccggatcgag tggccgacga tccggttgag
      661 gagcttcacc acggcgtaga tggcgaccag tgtcaccagg acctgaagca ccgtgaggag
      721 cgaaacccgg gtctggccgg cggtgaagcc gacgcggtcg agcgtgtcgg tgatgggctt
      781 cagcccgccg acggcgcggg cgagcaggga gacgaaggtc acgccggcga gcagggccgc
      841 gatccagcga ggcatctgca ggccccgcac caggcccgaa acaagcagcg ccgcccaggc
      901 ggccaaagcg gcccccagca ccatggctcc gagcggccgc caagcatccg cgttgagcgc
      961 gatcgcgacg acgagcgcgc cggtgaggta acgggtgaga tcgcacatgc gacgggcgag
     1021 tgcctcgccg cggccgcccg cgcgatgctc ccagaaagcc gccacgcgcg gcccgagcca
     1081 gcgcgacgca agccatgcga gcccccaggc caaagcgact aacgcgacgg cgatggcggc
     1141 cacgatccag tcgccggagg tcggccaggt caccccgaac ctctcgaaca ggcccctcat
     1201 tccgaagcgc catagccggc gagggccgcg gccagatcct cgatcaggtc gtccggatcc
     1261 tccagcccga tatggagcct caccagcggc ccctccgccc gccaccgcgt gacggtccgg
     1321 aggcgctccg gatcgaccgg gacggcaagg ctttcatagc cgccccagct gtagccgagg
     1381 ccgaacagat cgagcgcctc gacgaaccgg gtacgctccg cctcgtcgcc gccgttcagg
     1441 acgaaggtga agagccccga cgagcccctg aagtcccggg tccattcttc gtggccaggg
     1501 cagtcgggaa gcgcgggatg gagcacggcc gccacttgcg gctgctcctt cagccatcgg
     1561 gcgaccctga gcgcgctcgc ctcatgccgg cgaaggcgga cgcccatcgt cctgaggccg
     1621 cgcgagcaga gccaggcatc gtcggggctg accgactggc ccaggtccca gctcgtccgc
     1681 tccagcttgg gataccagcg cgcgccggcg gtgacggagc cgagcatgac gtcggcgtgg
     1741 cccccgacat atttggtccc ggcgaggatc gtgaggtcca ccccggcggc aagggccggg
     1801 aagaagagcg gagtggccca ggtattgtcg agcagcgtcg ccacgccctt cgccttcgcg
     1861 gcggcgcaga tggcgggaac gtcctgcacc tccatggtga gcgagccggg gctttcgagg
     1921 aagaccgcct tcgtgcgctc gccgatcaga tcggcgatcc cggcgccgac cagtggatcg
     1981 taatagcggg tggcgacccc gtagcgcttg aggaagacgt cgcagaagcg gcgggtgggg
     2041 ccgtagacgc tgtccgtcat cagcagctcg tcgcccgccg acagcaccga cagcagcgcc
     2101 gcggtcaccg ccgccagacc gttgggatag agcatcgtcc cggccgcgcc cggctccagc
     2161 tcggtcagcg cttccgaaag cgcccagtgg gtcggagtgc cgtggaggcc gtaataatat
     2221 tcgccgaacg cgggcgcggc ggcgcgcatc tcggccacgg tgtcgaagag gatggtcgag
     2281 gcccggtgga ccggcgggtt gaccgtccgg ccgcgccatt cctcgcggcg gccgccgtgg
     2341 acgagcttcg tgtccggccg cccgtccttc acgccggccc cgtcgccttg ggagccgcgg
     2401 gatccatgcc ccattcggac cagctcccgt cgtagagccg gacgtcctcc ttgccgagca
     2461 ggtgggcgcc gaagagcagg acgcaggcgg tgatgccgga gccgcaggtg gcgaccatcg
     2521 gcttgccgag gtccaccccc gcggattcga aggcggagcg aagctcgtcg ccctgcttcc
     2581 agctgttgtc gtcgctgaac agcctgccct gcgggaggtt cttcgagccc ggtatgtggc
     2641 cggaagccat tccgggccga ggctcggctt cctcgccggt gaaccgcccg gggccgcgcg
     2701 cgtcgacaat ctcatgacgg ccgtcgccca gcaggcccgc caccgtcgcc ttgtccgcca
     2761 ccgcgcccgc ctcgaagctc gggctgaaat ggccgtggcg tacctgcggc cggcccgctt
     2821 ccagcggccg cccttccgcc ttccacttct gaagcccgcc gtcgaggatc gccacgtaat
     2881 gggcgccgaa gctcttcagc atccaccagg cgcgggcggc gctgtgaagc gggctgttgt
     2941 cgtagacgac gaaccgctgg ccgtcgcgaa gcccgaggga ggccatacgg ctcgcgaact
     3001 tgggc
//
LOCUS       DASWNU010000083         7215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_444663, whole genome shotgun sequence.
ACCESSION   DASWNU010000083 DASWNU010000000
VERSION     DASWNU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444663"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /gene="lon"
                     /locus_tag="VGB57_01630"
     CDS             complement(<1..1744)
                     /gene="lon"
                     /locus_tag="VGB57_01630"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0130075.1"
                     /translation="MTQSYPVLPLRDIVVFPQMIVPLFVGRDKSVAALESAMAADKQI
                     FLVSQLDPAEDDPDRDALYDMGVVATVLQLLKLPDGTVRVLVEGQQRANLVSMGMEDQ
                     HLVAEVEPIEVDEPEGAEAAALMRSVVEQFENYSKLNRKLPAETAVQLAQIEEASKLA
                     DSVAANINIKVSDKQALLAELNPVRRLEMVFAFMEGELGVLQVEKRIRSRVKRQMEKT
                     QREYYLNEQLKAIQRELGNEGEEGEGDELAELARKIAKTKLSKEARSKATSEMKKLKA
                     MAPMSAEATVSRNYLDVLLGLPWGKKSKVKKDIAEAQAILDEDHYGLEKVKDRIVEYL
                     AVQARTNKLKGPILCLVGPPGVGKTSLGRSIARATGREFVRQSLGGVRDEAEIRGHRR
                     TYIGSLPGKIISNLKKAGSSNPLFLLDEIDKLGQDFRGDPASALLEVLDPEQNSKFQD
                     HYLEIDYDLSDVMFVTTANTMDMPQPLLDRMEIIRLEGYTEDEKVEIATRHLIPKQIE
                     SHGLKEGELTFAKDGLRSLIQHYTREAGVRTLEREIAKVARKVLRRILEGKTERVELT
                     AENIGEYSGVRRHRH"
     gene            2091..2594
                     /locus_tag="VGB57_01635"
     CDS             2091..2594
                     /locus_tag="VGB57_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130076.1"
                     /translation="MFAFVPLLLLSVQPPVYAKERNSDSAGQKCTPKKKKSGGMFGAI
                     AGGIAGSALGGWGGAPGALIAAGLPVASLLTDAIIRKLDCKEQVQAATATDNAVRGGV
                     GTTTSWESESRPGVKGSSTVRDQRASADGGSCMLITDVVIVDGEETTVDKRMCRKPGG
                     GNYVLAA"
     gene            2599..3309
                     /locus_tag="VGB57_01640"
     CDS             2599..3309
                     /locus_tag="VGB57_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130077.1"
                     /translation="MRGGIAFLALTGLLSSSWAQAAVDPANPTCPRELNFSAHKAMQF
                     TLRQEGGMRVLLAEGAIDSGAPARLAAALKKHEPIDQIWLRSPGGDARAGTEAGKLIR
                     QSLAFTRIPDGWACFSACNFVFMGGRGRIVDPGGQFVVHMFTHTSDRGAIEAEVLRGT
                     ESTVGLIGDIEQDSALLASEDNDFLIRMGVSRKLLTEIMYRQKAVKEQGGDGSTRRCL
                     TQAEVFQYKVANARAEGK"
     gene            3353..5320
                     /locus_tag="VGB57_01645"
     CDS             3353..5320
                     /locus_tag="VGB57_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY0130078.1"
                     /translation="MRASALTALAGTLLAAAPAAAEPVPATGPAPAWVVAPAVPPLDD
                     SAGDAPFQILMNSAQERILSDGVEHYIEYAAVPLTTGGLHALGNVTLPWNVERTDLTL
                     HKIAIRRGGATIDLLKSAETLVLRRENNLEKAMLDGTWTVVIPAWGLQVGDILNVAFT
                     YKTKPSTLGLQPEELQDLVTTVPIAWMERRFLVADDVKLDWKLSPTIGSPQIVRRAGM
                     TEHRFVATKVKPVSTPSFAPSRYTAPLIQVTGYRDWASVAELIRPLFDKSRRPNPASP
                     LLREADRIAAATSDPGERMIAALRLAQEQVRYVALLLGDGAYVPMSADDTWHRKFGDC
                     KGKTSLLLALLDRLGIDAEPMLVSNQNDDRLKEQLPSLQLFDHVLVRARIGSRIYYLD
                     AVGYGQRTLDELAITPFSQGLALRGGARLEELVQGELAAPTREFSLAWDGSKGLEGEV
                     PFEGILTLRGTAAAAMRAKLSGATDVSQFDTGLKALFPRIHNDNLNILEKRPDGPAGS
                     FVVRFGGKSAMDWSSFQGKRDARYAFTQSTLTWQPDFDRSSGPGKEWPVFITSDPHWE
                     RMTETIILPDGGKGYSLEAGAVDKTAGGSKLSRTVTKSGERVTMVAEFRHLKREITAE
                     EARAATPLLEEISQDYAYVVGPPIRKGKAGR"
     gene            complement(5328..5837)
                     /locus_tag="VGB57_01650"
     CDS             complement(5328..5837)
                     /locus_tag="VGB57_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130079.1"
                     /translation="MNSDPALAGRPAWLAWLPRFLFETDPFPARYVAKAWLTALLPSI
                     ALSALVRLIAADAAALQFPGEAAPLILLVVFVGPALETGLLALPLLALNRFLGPGPAV
                     VGSALIWAVAHSLVAPVWGLVIWWPFLIMSIAFLTWRRQGLLWALGVAFAIHALQNGF
                     AVLLVLAAG"
     gene            complement(5854..7116)
                     /gene="clpX"
                     /locus_tag="VGB57_01655"
     CDS             complement(5854..7116)
                     /gene="clpX"
                     /locus_tag="VGB57_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY0130080.1"
                     /translation="MPNLRDGDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     NDIIREETKSSLVKTRDGVPTPLEICKHLDDYVIGQSHAKRVLSVAVHNHYKRLAHGS
                     KGGEVELSKSNILLVGPTGCGKTLLAQTLARLLDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQASDYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLMEGTT
                     ASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIIADRLQGKSIGFGAYVAAPE
                     ERRTGEILRQGEPDDLLKFGLIPEFVGRLPVIATLEDLDEVALVKILREPKNALVKQY
                     AKLFDMEDVKLSFTDDAMVSVAKKAIERKTGARGLRSILENILLDTMFDLPSMNGVDE
                     VMIDKDVVEGRKDPIRVYAEKEKTGDAA"
BASE COUNT         1218 a         2364 c         2386 g         1247 t
ORIGIN      
        1 cgtgacggtg gcggcgaacg cctgaatatt caccaatatt ctcggcggtt agctcgaccc
       61 gttcagtctt gccctcaagg attcgacgaa gcaccttgcg cgccaccttc gcgatttccc
      121 gttccagcgt gcgaacgccc gcctcgcggg tataatgctg gatcaggctc ctgaggccgt
      181 ccttcgcgaa ggtcagctcg ccttccttga ggccgtgcga ttcgatctgc ttcgggatga
      241 ggtggcgggt ggcgatctcg accttctcgt cctcggtata gccttcgagg cggatgatct
      301 ccatccggtc gaggagaggc tgaggcatgt ccatcgtgtt cgccgtggtg acgaacatga
      361 cgtcggacag gtcgtaatcg atctcgagat aatggtcctg gaacttcgag ttctgctccg
      421 ggtcgagcac ctcgagcagg gccgaggcgg gatcgccgcg gaaatcctgg ccgagcttgt
      481 cgatctcgtc gagcaggaag agcgggttcg agcttcccgc cttcttcagg ttcgagatga
      541 tcttgcccgg aagcgagccg atataggtgc ggcggtggcc gcggatctcg gcctcgtcgc
      601 gcactccgcc cagtgactgg cggacgaact cgcggcccgt cgcccgggcg atcgagcggc
      661 cgagcgaggt cttgccgacg cccggggggc cgaccaggca caggatcggg cccttcaatt
      721 tgttggtccg ggcctggacc gcgagatatt cgacgatccg gtccttgacc ttctcgagcc
      781 cgtaatgatc ctcgtcgagg atcgcctggg cctcggcgat gtccttcttg accttcgact
      841 tcttgcccca gggcaggccg agcaggacgt cgagatagtt acggctgacg gtcgcctcgg
      901 ccgacatcgg cgccatcgcc ttcagcttct tcatctcgga ggtcgccttg gagcgggcct
      961 ccttcgagag cttggtcttg gcgatcttgc gggcgagctc ggcgagctcg tcgccctccc
     1021 cttcctcgcc ttcattgccc agctcgcgct ggatcgcttt caactgctcg ttgagataat
     1081 attcgcgctg ggtcttctcc atctgccgct tgacccggct tcggatgcgc ttctcgacct
     1141 gaagcactcc aagctcgccc tccatgaagg cgaacaccat ctccagccgg cgaaccggat
     1201 tgagctcggc gagcagcgcc tgcttgtcgg agaccttgat gttgatgttg gcggcgaccg
     1261 agtcggcgag cttcgaggct tcctcgatct gcgcgagctg aaccgcggtc tcggccggca
     1321 gcttgcggtt gagcttcgag taattctcga attgctcgac caccgagcgc atcagcgccg
     1381 cggcttcggc gccctcgggc tcgtcgacct cgatcggctc gacctcggcg acgagatgct
     1441 ggtcctccat ccccatcgag acgaggttcg ctcgctgctg gccctcgacc agcacccgca
     1501 cggtcccgtc ggggagcttg aggagctgaa gaacggtcgc gaccacgccc atgtcgtaga
     1561 gcgcgtcgcg gtcgggatcg tcctcggccg gatcgagctg cgagacgagg aatatctgct
     1621 tgtccgccgc catcgcgctc tcgagcgcgg cgaccgactt gtcgcggccg acgaagagcg
     1681 gcacgatcat ctgcgggaag acgacgatgt cgcgaagggg caggacggga taggattggg
     1741 tcatcgaaac tccacatgca ggcccttgag gccttcttgc aacctatatg gggtgttggg
     1801 gccgcgcatc aataaacctg cgtcccggcg aaggccggga cccggcctcg acccgccggg
     1861 ccccggcttt cgccggggag caccgaggcg tcgattgccc ctttccgacc ctcttccaat
     1921 atggcaaacc tgccaagggt ttaggggtta tccgcctacc cggaagcagg gggctgcgac
     1981 aattgacaca cacgcgccga gcccgcggca tgaagccgca acaaggccgg attagcgccc
     2041 tttcgagagg ccggtcaggc acatcgggga gttattctat gcgtcggtac atgttcgcgt
     2101 tcgtcccgct tctgctgctc tcggtgcagc cccccgtcta cgccaaggag cgcaactccg
     2161 actcggccgg gcagaaatgc acgcccaaga agaagaaaag cggcggcatg ttcggcgcca
     2221 tcgccggcgg catcgccgga tcggcgctgg gcggctgggg cggggctccg ggcgccctga
     2281 tcgccgccgg cctgccggtc gcgtcgctgc tcaccgacgc gatcatccga aagctcgact
     2341 gcaaggagca ggtccaggcc gccacagcga cggacaatgc cgttcgcgga ggcgttggaa
     2401 ccacgacgag ctgggagagc gagagccggc ccggagtgaa gggttcctcg actgtccgcg
     2461 accagagggc ttcggccgac ggcggcagct gcatgctgat caccgacgtc gtcatcgtcg
     2521 atggcgagga gacgacggtc gacaagcgca tgtgccgcaa gcccggcggc ggcaattacg
     2581 tgctggcggc ctgaggcgat gcgggggggc atcgcttttc tcgctctgac gggcctgctt
     2641 tcgtcttcct gggcgcaggc ggccgtcgat ccggccaatc cgacctgtcc cagggagctc
     2701 aacttctcgg cccacaaggc gatgcagttc acccttcgcc aggagggagg aatgcgggtc
     2761 ctcctggcgg aaggcgcgat cgacagcggc gcccccgcgc ggcttgcggc ggcgctcaag
     2821 aagcatgagc cgatcgacca gatctggctg cgttcgccgg gtggagacgc ccgggcgggc
     2881 accgaggccg gtaagctgat ccgccagtcg ctggccttca cccgaattcc ggacggctgg
     2941 gcctgcttca gcgcctgcaa tttcgtgttc atggggggcc gcggccgaat cgtcgatccg
     3001 ggcggccagt tcgtggttca catgttcact cacaccagcg accgcggcgc gatcgaggcg
     3061 gaggtgcttc gcggcaccga gagcacggtc gggctgatcg gcgatatcga acaggattcg
     3121 gccctgctcg ccagcgagga caacgatttc ctgatccgga tgggcgtgtc gcgcaagctt
     3181 ctgaccgaaa tcatgtaccg ccagaaagcg gtcaaggagc agggcggcga cggctcgacc
     3241 cgccgctgcc tgactcaagc cgaggtgttc cagtacaagg tcgcgaacgc gcgggcggag
     3301 ggcaaatagg cgcccgcgcc cggttgaacg gcttttgaaa ggggaatcga aaatgcgtgc
     3361 ctcagccttg acggccctcg ccggcacgct cctcgccgcc gctccggccg ccgcagagcc
     3421 ggtcccggcc accggcccgg ctccggcctg ggtggttgct ccggccgttc cgccgctcga
     3481 cgattccgcc ggcgacgcgc ccttccagat cctgatgaac tcggcccagg agaggatcct
     3541 gtcggacggg gtcgagcatt atatcgaata tgccgcggtc ccgctgacca ccggcgggct
     3601 gcacgcgctc ggtaacgtca ccctgccctg gaatgtggag aggaccgacc tcaccctcca
     3661 caagatcgcc atccggcgcg gcggcgcgac catcgatctg ctcaagtccg ccgagacgct
     3721 cgtccttcgc cgcgagaaca atctcgaaaa ggccatgctc gacggcacct ggacggtcgt
     3781 gatcccggcc tggggccttc aggtcggcga cattctcaac gtggccttca cctacaagac
     3841 gaaaccctcg accctcggcc tccagcccga ggagctccag gatctggtca cgaccgtgcc
     3901 gatcgcctgg atggagcgcc gcttcctcgt cgcggacgac gtcaagctgg actggaaatt
     3961 gtcgcccacg atcggttcgc cccagatcgt ccgccgggcg ggcatgacgg agcaccgctt
     4021 cgtcgcgacg aaggtgaagc cggtctcgac tcccagcttc gccccgtcgc gctataccgc
     4081 gccgctgatc caggtcaccg gctatcggga ctgggcttcg gtcgcggagc tgatccgtcc
     4141 gctgttcgac aagtcccgcc gtcccaaccc cgcctccccg cttctgcgcg aagccgaccg
     4201 gatcgccgcg gcgacgagcg acccgggcga gcggatgatc gccgcgctgc gcctggctca
     4261 ggagcaggtc cgctacgtcg ccctcctgct cggcgacggc gcctatgtcc cgatgagcgc
     4321 cgacgacacc tggcaccgca agttcggcga ttgcaaaggc aagacgagcc tgctgctcgc
     4381 gcttctcgac cggctgggga tcgacgccga gccgatgctc gtcagcaacc agaacgacga
     4441 caggctgaag gagcagctcc cttccctgca gctgttcgat cacgtcctgg tgcgggcgcg
     4501 catcggctcc aggatctatt atctggatgc ggtcggctac ggccagcgga cgctggacga
     4561 gctcgccatc acgcctttct cccaaggcct tgcgctccgc ggcggggccc ggctcgaaga
     4621 gctcgttcag ggcgagcttg ccgctccgac tcgggaattc tcactggcct gggacgggtc
     4681 gaagggcttg gagggcgagg tccccttcga ggggattttg accctgcgcg gtacggcggc
     4741 cgccgcaatg cgggccaagc tttccggcgc gaccgacgtc tcccagttcg atactggcct
     4801 caaggcgctg tttccgcgga ttcacaacga caatctgaac atcctggaga agcgtccgga
     4861 cggcccggcg ggcagcttcg tcgtccgctt cggcggcaag tcggcgatgg actggtcgtc
     4921 gttccagggc aagcgcgacg cccgctacgc cttcacccaa agcactctca cctggcagcc
     4981 cgacttcgac cggagctcgg gacccggcaa ggaatggccg gtcttcataa cgtccgatcc
     5041 gcattgggaa cgcatgacgg agaccatcat cctgcccgac ggcggcaaag gctattcgct
     5101 cgaggcgggc gcggtggaca agacggccgg cgggtccaag ctcagtcgca ccgtgacgaa
     5161 gtcgggggag cgagtgacga tggttgccga gttccggcat ctgaagcgcg agatcaccgc
     5221 cgaggaagcg cgtgccgcca cgcccttgct cgaggagatc agccaggatt acgcctacgt
     5281 cgtcgggccg ccgatccgga aggggaaagc cgggcggtag cggtccgtca gccggccgcc
     5341 aggacgagaa gaaccgcgaa gccgttctgg agcgcgtgga tagcgaaggc cactcccagc
     5401 gcccaaagca ggccctggcg gcgccaggtc aagaaggcga tcgacatgat caggaagggc
     5461 caccagatca ccaggcccca gaccggcgcg acgaggctgt gcgcgacggc ccagatcagg
     5521 gcgctgccga cgaccgccgg gccgggcccg agaaaccggt tcagagcgag cagaggcaag
     5581 gccagaagcc cggtttcgag tgccggaccg acgaagacga caagcaggat caaaggcgcc
     5641 gcctcgcccg ggaattgcag cgccgcggcg tcggcggcga tcagccggac cagcgccgaa
     5701 agggcgatgc tcggcagcag cgccgtcagc cacgccttgg cgacgtaacg ggccgggaac
     5761 gggtcggtct cgaagaggaa gcggggcagc cacgccagcc aggcgggccg cccggccaag
     5821 gcgggatcgc tattcaccgt cgacgcctcg cgctcaggcg gcgtcgccgg tcttttcctt
     5881 ttcggcatag acccggatcg ggtccttgcg gccctcgacc acgtccttgt cgatcatcac
     5941 ctcgtcgacc ccgttcatcg acggcaggtc gaacatcgtg tcgagcagga tattctcgag
     6001 gatcgagcgg aggccgcggg cgccggtctt gcgctcgatc gccttcttgg cgacggagac
     6061 catggcgtcg tccgtgaagc tgagcttcac gtcctccatg tcgaacagct tcgcatattg
     6121 cttgaccaga gcgttcttcg gctcgcgcag gatcttgacc aaagcgacct cgtccaggtc
     6181 ctcgagggtc gcgatcaccg gaagccggcc gacgaactcg gggatgaggc cgaacttcag
     6241 gagatcgtcc ggctcgccct ggcggaggat ctcgccggtg cggcgctcct cgggcgcggc
     6301 gacataggcg ccaaagccga tggacttgcc ctggagacgg tcggcgatga tcttctcgag
     6361 gcccgcgaag gcgccgccgc agatgaagag gatgttggtc gtgtcgacct ggaggaattc
     6421 ctgctgcgga tgcttgcgcc cgccctgcgg cggaacggaa gcggtggtgc cctccatcag
     6481 cttgaggagc gcctgctgga cgccctcgcc cgagacgtcg cgggtgatgg acggattgtc
     6541 cgacttgcgg ctgatcttgt cgatctcgtc gatatagacg atgccccgct gcgcccgctc
     6601 gacattgtag tccgaggcct ggagcagctt caggatgatg ttctcgacgt cctcgccgac
     6661 atagccggct tcggtcagag tcgtcgcatc ggccatggtg aagggcacgt cgaggagacg
     6721 cgccagcgtc tgcgcgagca aggtcttgcc gcagccggtg gggccgacga gcaggatgtt
     6781 cgacttgctg agctcgacct cgccgccctt ggagccgtgg gcgaggcgct tgtaatgatt
     6841 gtggaccgcg accgagagaa cgcgcttggc gtggctctgg ccgatcacgt aatcgtcgag
     6901 atgcttgcag atttcgagcg gcgtcggaac gccgtcgcgg gtcttcacca gcgaggactt
     6961 ggtctcctcg cggatgatgt cgttgcacag ctcgacgcat tcatcgcaga tgaagacggt
     7021 cgggccggcg atcagcttgc ggacctcatg ctggctcttg ccgcagaagg agcaatagag
     7081 ggtgctcttg ctgtcgccgt cacgcaggtt tggcattcat tcttccttac aggccaccgg
     7141 tccgagcggc acccaagcag cccatcctga cggcaaatct agtcgatcac aatggcctaa
     7201 acgttaagca gccga
//
LOCUS       DASWNU010000084         4822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_185841, whole genome shotgun sequence.
ACCESSION   DASWNU010000084 DASWNU010000000
VERSION     DASWNU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185841"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..237)
                     /locus_tag="VGB57_01660"
     CDS             complement(4..237)
                     /locus_tag="VGB57_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130081.1"
                     /translation="MTPQDDETLWRNRFIAINLVRIGGTVVVLIGLAIWHTDFLVEDG
                     TLVLGLPITLAGLAASFWGPKWLAARWRTPPGP"
     gene            complement(234..857)
                     /locus_tag="VGB57_01665"
     CDS             complement(234..857)
                     /locus_tag="VGB57_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY0130082.1"
                     /translation="MGKGLPMHPNRKFHIGEREAMAALVRDLGFGVLFVSTPDGPRAV
                     HVPVLLDGDRLRFHVSRGNAVHSTLAAGADSLFVGSGPHAYISPEWYGLEDRVPTWNY
                     VAVELEGPVRPLDPEALVQMLDEMSAEQEARLAPKPAWTRAKMSEGRFDGLLKSIAGF
                     EMRVSAWRGTAKVDQDKPQEVRTRIADALAERGEVDMAATMRMKALS"
     gene            complement(948..2039)
                     /locus_tag="VGB57_01670"
     CDS             complement(948..2039)
                     /locus_tag="VGB57_01670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0130083.1"
                     /translation="MSDVRQFIVAPDDDGIRLDRWFKRHLPEASFNTVSRWARTGQLR
                     IDGKRAAPGDRVEAGQSIRVPPAEPAAAAAPARAKPERKALSEDEIAFVNEIVIHRDA
                     QAIVVNKPPGLATQGGTKTLTHLDGLLDGLADDDGNRPKLVHRLDKDTSGVLLLARTA
                     RSAGFFSKAFSGRTARKVYWAIVTGVPDIADGFIDLPLAKQPGTGGEKMHVEEGEGGL
                     TARTRYRMIERAGNRAAWLELQPLTGRTHQLRVHMAAIGHPILGDGKYGGPDAFLTGA
                     ISRKLHLHARRLRIEHPDGDTLDVTAELPSHFAETLANLGFDPELSDLPLDEIKFSET
                     AEGKRRAASAAAKSRRKERKGERRGRGQR"
     gene            complement(2208..2858)
                     /locus_tag="VGB57_01675"
     CDS             complement(2208..2858)
                     /locus_tag="VGB57_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130084.1"
                     /translation="MTDRAFPLLRLTALGAALALAACDRGGGESNLADLANDADPAVT
                     SALNDQILVDENLATQSNKNAARPSPTPAAASYPAPDASPSAPGKAASPPAALAGPKG
                     CSDARRFDYNMAWAGKLSPNFPVYPGGKVTEAAANNSGGCSTRVVTFATAENWQRVLD
                     WYHTRAVRAGYSSEHQVREGDHILAGSNEGDKGAFYLIVTPKPKGSEVALIANNGR"
     gene            complement(2922..3308)
                     /locus_tag="VGB57_01680"
     CDS             complement(2922..3308)
                     /locus_tag="VGB57_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0130085.1"
                     /translation="MAALADPEEVRLHLVAAARAGIALSYGELLERLGYSFSRPKMRA
                     LCKILAVVDEAAALRGEPELAVLVVRQSDGLPGQGWWIAGGARSGGYDGPWEGPEARR
                     FIDRVQSESFAFWKSRSGTDGTDSPG"
     gene            complement(3320..3850)
                     /locus_tag="VGB57_01685"
     CDS             complement(3320..3850)
                     /locus_tag="VGB57_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130086.1"
                     /translation="MNRRSLCTAFAAAASLCGCDSNGGAGQPGNGAADGNVSAEGKSE
                     EGKISLKMPGLDMTLSLPKGVADEARAERDSKLLYPGATLRGMAIAAGPDSDKSGDSE
                     VEIRFSTPDPVGAVAAWYRDPARSGGFQLRGAAREGDSWLLTGVQKRDRHSFKLRLSP
                     RSGGGTNGQLTVRHRD"
     gene            4032..>4822
                     /gene="rarD"
                     /locus_tag="VGB57_01690"
     CDS             4032..>4822
                     /gene="rarD"
                     /locus_tag="VGB57_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY0130087.1"
                     /translation="MTDAARTRSGLLMGLGAYALWGVLPLYFKALIHVAATEIVAHRI
                     LWSLIFLGTLATLWKRWPRIRAALTNPRVALTLLLTSVLIGINWLVYIYAVVSGHVLE
                     GSLGYYLNPLVNVLLGVFLLKERLSRLQKAAVFLAGAGVAILAFGAGGAIWISLTLAA
                     SFAVYGFLRKVAPVESLEGLWIETLFLAPLALGWVLWLTQAGDSAFLDSRVTDLLLIL
                     GGALTAIPLLLFTAAAKKLPYSTLGFLQYVAPSLQFLLAVLVFDEP"
BASE COUNT          698 a         1637 c         1652 g          835 t
ORIGIN      
        1 gcttcatggg ccgggcggcg tcctccagcg ggccgcgagc catttcgggc cccagaagct
       61 ggcggcgagg ccggccaggg tgatcggcag cccgagcacg agcgtcccgt cctcgaccag
      121 gaaatcggtg tgccagatgg cgaggccgat caggacgacg accgtgccgc cgatccgcac
      181 caggttgatc gcgatgaagc ggttgcgcca cagggtctcg tcgtcttgcg gggtcatgag
      241 agcgccttca tcctcatcgt cgccgccatg tccacttcgc cccgctcggc gagcgcgtcc
      301 gcgatccggg tccgtacctc ctgcggcttg tcctggtcca ccttggccgt gccgcgccac
      361 gccgagaccc gcatttcgaa gccggcgatc gacttgagca gcccgtcgaa gcggccctcg
      421 ctcatcttgg cgcgagtcca ggccggcttc ggcgccagcc gcgcctcctg ctcggccgac
      481 atctcgtcga gcatctggac cagcgcctcg ggatcgagcg gccgcaccgg cccttccagc
      541 tcgaccgcga catagttcca ggtcggcacc cgatcctcca gtccatacca ttcggggctg
      601 atataggcat gcgggccgct gccgacgaac agcgagtccg cgccggccgc cagagtgcta
      661 tggaccgcgt tgccgcgcga cacgtggaaa cgcagccggt cgccgtcgag cagcaccggc
      721 acgtgcacgg cccgagggcc gtcgggggtc gacacgaaca gcactccgaa gccgaggtcg
      781 cggaccagcg ccgccatcgc ctcgcgctcg ccgatgtgga atttgcggtt ggggtgcatc
      841 ggaaggccct tacccacccg tcattccatg aacaccgacg cccgcgtatt tttcgcggaa
      901 cgccgtgttc atggatgccg ggtcgagccc ggcatgacgg acctccatca cctttgcccc
      961 cttccccgcc gttcgccctt gcgctccttg cggcgcgact tggcggcggc ggaggcggcg
     1021 cgccgcttgc cttcggcggt ctccgaaaat tttatctcgt cgaggggcag atcgctcagc
     1081 tccgggtcga agccgagatt ggcgagagtc tcggcgaagt gcgacggcag ctcggcggtg
     1141 acgtcgagcg tgtcgccgtc gggatgctcg atccgcaggc ggcgggcgtg gagatggagc
     1201 ttgcggctga tcgctccggt gaggaaggcg tcgggtccgc catatttgcc gtcgccgagg
     1261 atggggtggc cgatcgccgc catatggacc ctcaactggt gggtgcggcc ggtcagcggc
     1321 tgaagctcga gccaggcggc gcgattgccg gcccgctcga tcatccgata gcgggtcctc
     1381 gcggtaagcc ccccctcgcc ttcctcgacg tgcatcttct cgccgcccgt gccgggctgc
     1441 ttggcgagcg gaaggtcgat gaagccgtcg gcgatgtcgg gcactccggt gacgatcgcc
     1501 caatagacct tgcgcgcggt acggccggaa aaagccttgg agaagaagcc ggcggaccgg
     1561 gcggttcggg cgaggaggag gactcccgaa gtatccttgt cgaggcggtg gacgagcttc
     1621 gggcggttgc catcatcgtc ggcgagcccg tcgagcaggc cgtcgagatg ggtgagcgtc
     1681 tttgtgcccc cctgggtggc gaggcccggc ggcttgttga cgacgatcgc ctgggcatcg
     1741 cggtggatga cgatctcgtt gacgaaggcg atctcgtctt cgctcaatgc tttgcgttcg
     1801 ggtttcgctc gcgccggagc ggcggcggcg gcgggctcgg ccggaggaac ccggatcgac
     1861 tggccggcct cgacccggtc gcccggcgcc gcgcgcttgc cgtcgatgcg cagctgcccg
     1921 gtccgggccc agcgcgacac cgtgttgaag ctcgcctccg gcagatggcg cttgaaccag
     1981 cggtcgaggc ggatgccgtc gtcgtcgggc gcgacaatga actggcggac gtcgctcatg
     2041 gcggcgatgt agcggggcgc gagagatggc gccagacgtt cgtcaccgcc gtcgtcccgg
     2101 gagggacccc atcaaagtac tattgtgatg ggaacccgaa agccgggacc tcggggagac
     2161 ccctgagact tcttgtcctg agatcccggc tttcgccggg acgacgatca gcgcccgtta
     2221 ttggcgatca gcgcgacttc ggagcctttg ggcttggggg tgacgataag gtagaaagcg
     2281 cccttgtcgc cctcattgct tccggcgagg atatgatcgc cttcacgcac ctgatgctcc
     2341 gacgaatagc cggcgcgcac ggcccgcgta tggtaccagt cgagcacgcg ctgccaattc
     2401 tccgccgtcg cgaaggtcac gacacgggtg ctgcagccgc cggagttgtt cgcggccgcc
     2461 tcggtcacct tgccgcccgg atagaccggg aagttgggcg acagcttgcc cgcccaggcc
     2521 atgttatagt cgaagcgccg ggcgtccgag cagcccttgg ggccggcgag cgccgcggga
     2581 gggctggcgg ccttgcccgg agccgacggg gacgcatcgg gcgccggata ggaagccgcg
     2641 gcgggggtcg gcgagggccg ggcggcattc ttgttggact gggtcgccag attctcgtcg
     2701 acgaggattt gatcgttgag ggcgctggtc accgccggat cggcgtcgtt ggcaaggtcg
     2761 gcgagattgc tctcgccgcc gccgcggtcg caggcggcga gcgcgagcgc cgctcccagg
     2821 gcggtcaggc gaagcagcgg aaaagcgcgg tcggtcatcg aagagactcc ttggagcccc
     2881 gctatgccgc attcccacgc aaaatgggtg acgagagatg gttaaccggg ggagtccgtg
     2941 ccgtctgtcc cgcttcggga cttccagaag gcgaagcttt cggactggac ccggtcgatg
     3001 aaacgccgcg cctctggccc ttcccagggg ccatcatagc cgcccgagcg ggcgccgccg
     3061 gcgatccacc agccctggcc cggaagcccg tcgctctggc ggacgaccag caccgccagc
     3121 tcgggctcgc cgcgcagcgc agcggcctcg tcgacgacgg cgaggatctt gcagagcgcc
     3181 cgcatcttgg gcctcgagaa ggaatagccg agccgctcga gcagctcgcc atagctgagc
     3241 gcgatcccgg ccctggccgc ggcgacgaga tgaagccgca cttcctcagg atcggcgagc
     3301 gcggccaatc gcccctccct tagtcgcggt ggcggaccgt gagctggccg ttggtgcccc
     3361 cgccggaacg ggggctcagg cgaagcttga aactatgcct gtcgcgcttc tgcacgccgg
     3421 tgaggagcca cgagtcgccc tccctggccg caccccgcag ctggaacccg ccggaccggg
     3481 ccggatctcg gtaccaggcc gcgaccgcgc cgaccggatc cggagtggaa aagcggatct
     3541 cgacctcgct gtcgccgctc ttgtcgctgt ccggaccggc ggcgatggcc attccgcgca
     3601 aggtcgcccc cggatagaga agcttgctgt cgcgctcggc ccgagcctcg tcggcgacgc
     3661 ctttgggaag gctgagagtc atgtcgaggc cgggcatctt gagcgagatc ttgccctcct
     3721 cggatttgcc ctccgccgaa acgttgccgt cggccgctcc attgcccggt tggccggcac
     3781 ctccattcga atcgcacccg caaagcgaag cggcggcggc gaaggcggtg caaaggcttc
     3841 gacgattcat tctctctccc cgggtcatga ccgatcctag agggcgtttc gatcggattg
     3901 aagcccttgt tccccggcga aggcccgggt ccagctcctc ccgccagcga gctggccccg
     3961 gccttcgccg gggaacgctt cggcttgcca aatgcttgtc gcggcccccg ccctcggcta
     4021 cccgcccgcc ggtgaccgac gccgcccgaa cccgatccgg gctcctgatg gggctgggcg
     4081 cttatgctct gtggggtgtg cttccgctct acttcaaggc gctgatccac gtcgcagcga
     4141 ccgagatcgt cgcccaccgg atcctctggt cgctcatctt cctcggaacg ctcgccacct
     4201 tgtggaaaag gtggccgcgg atccgagcgg ctttgaccaa cccccgggtc gccctcaccc
     4261 tcttgctcac ttcggtgctg atcggaatca actggctggt ctacatctac gcggtcgtca
     4321 gcgggcatgt ccttgagggc agcctcggtt attatctgaa cccgctcgtc aacgtgctgc
     4381 tcggggtctt cctgctcaag gagaggctga gcaggttgca gaaggcggcg gtgttcctgg
     4441 cgggcgccgg ggtcgccatc ctggccttcg gggcgggcgg cgcgatctgg atcagcctca
     4501 ccctcgccgc cagcttcgcc gtctatggtt tcctgaggaa ggtggcgccg gtcgagtcgc
     4561 tggaggggct ttggatcgag accctgttcc tggcgccgct cgccctcggc tgggtattgt
     4621 ggctgactca ggccggggac agcgccttcc tcgacagccg ggtcaccgac ctgctgctca
     4681 tcctcggcgg agcgctcacc gccatcccgc tgctgctctt cacggcggcg gcgaagaaat
     4741 tgccttattc gaccctgggc ttcctccaat atgtcgctcc gtcgctgcag ttcctgctcg
     4801 ccgtgctggt cttcgacgag cc
//
LOCUS       DASWNU010000085         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_185864, whole genome shotgun sequence.
ACCESSION   DASWNU010000085 DASWNU010000000
VERSION     DASWNU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185864"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGB57_01695"
     CDS             <1..261
                     /locus_tag="VGB57_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY0130088.1"
                     /translation="KCKAELGARPRMLGPEETLALTGHPIGGVCPFGLATRLPVYCDV
                     SLRDFDRVYPAAGSLNSSVEVTPERLFELVGERWVDLCRLPG"
     gene            321..947
                     /locus_tag="VGB57_01700"
     CDS             321..947
                     /locus_tag="VGB57_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEY0130089.1"
                     /translation="MRRLPLLLAPLLLAACTTNETGPAAAVPAAATGWRAVATDYDRE
                     RVRKWRTAWVRAIAKARAGGHSAEIAREGVLLEPDAALRGVAPPAGDYRCRVLKVGSQ
                     SEGLLDYVAYPYFDCRISGGAEAMDFVKVTGSQRPVGRLFSDSDRRLVFLGTLQLGDE
                     RGTLRYGHDRQRDMIGLLERIGERRWRLAFPYPAFESMLDVIELVPKE"
     gene            952..>2229
                     /locus_tag="VGB57_01705"
     CDS             952..>2229
                     /locus_tag="VGB57_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0130090.1"
                     /translation="MKRLLLLPLLAPLAFGAAPAAADPLADQVKQDLPSLMSLYRELH
                     ANPELSMQEVKSSARLAAEARKLGFEVTTGVGKTGVVAVMKNGPGPVLLMRADMDALP
                     VVEQTGLAFASKVKATSVSGTESGVMHACGHDTHMTGWLGTARRMAATKGQWSGTLVM
                     ILQPAEETGEGARAMLADGLFTRFPKPTHALAWHDSASLPAGVLGITVGPAFANVDTV
                     DIAVKGVGGHGAVPQNTRDPIVLASRIVGTLQTLVSRELDPQDAAVVTVGSFQSGTKH
                     NIISDQATLLLTVRSYSQKTRRALLDGIQRIARGEAIAAGMPEDRMPVVTIREGEFTP
                     AAINTDKLSADTAALFRTRFGADRVRVVPPPMVGEDFGRYHLADEKIESLIFWVGGVP
                     QSKWDAAGGDTSKLPSLHSPYWAPEAETVIATGI"
BASE COUNT          336 a          763 c          800 g          330 t
ORIGIN      
        1 aagtgcaagg cggagctcgg cgcccggccg cggatgctcg ggcccgagga gaccctggcc
       61 ctgaccggcc acccgatcgg cggagtctgc ccgttcgggc tggcgactcg gctgcccgtc
      121 tattgcgacg tttcgctcag ggacttcgac agggtctatc cggcggcggg atcgctgaac
      181 agctcggtcg aggtgactcc ggagcggttg ttcgagctgg tcggggagcg ctgggtcgac
      241 ctttgccggt tgccgggatg acgatctgcc ggaacggtcc attgatcgaa cggccgaagc
      301 gctcctatgg tcggcggatg atgcgccgcc tcccactgct ccttgccccg ctcctgctcg
      361 ccgcctgcac gacgaacgag accggtcccg ccgcggcggt tccggcggcg gcgacgggct
      421 ggcgggcggt cgccaccgac tacgaccggg agcgggtgcg caaatggcgg accgcctggg
      481 tgcgggcgat cgccaaggcg cgggcgggcg ggcattcggc ggagatcgcc agggaaggcg
      541 ttctgctcga gcccgacgcc gctctgaggg gcgtggcgcc gcccgccggc gattatcgct
      601 gccgggtgct gaaggtcgga agccagagcg aggggcttct cgactatgtc gcctatccct
      661 atttcgactg ccggatctcg ggcggagccg aagcgatgga cttcgtcaag gtcaccggct
      721 cgcagcggcc ggtcgggcgg ctgttttccg acagcgaccg gcggctcgtc ttcctcggca
      781 cgctccagct cggcgacgag cggggcaccc tgcgctacgg ccacgaccgc cagcgcgaca
      841 tgatcggcct tctggagcgg atcggcgagc gccggtggcg gctggctttc ccctaccccg
      901 ccttcgaatc catgctcgac gtgatcgagc tcgtccccaa ggaatgaacc gatgaaacgc
      961 ctgctgctgc tgcccctgct cgctccgctc gcctttggcg ccgcgccggc cgcggccgat
     1021 ccgctcgccg accaggtgaa gcaggacctg ccgtcgctga tgagcctgta ccgcgagctc
     1081 cacgccaatc ccgagctgtc gatgcaggag gtgaagagtt cggcgaggct cgccgccgag
     1141 gcgaggaagc tcggcttcga ggtcaccacc ggggtcggca agacgggggt cgtcgcggtg
     1201 atgaagaacg ggccgggccc ggttctcctg atgcgcgccg acatggacgc cttgcccgtc
     1261 gtcgagcaga ccggcctggc cttcgccagc aaggtcaagg cgaccagcgt ttccggcacc
     1321 gagagcggcg tgatgcacgc ctgcggccac gacacccata tgaccggctg gctgggcacg
     1381 gcccggcgga tggcggcgac gaaggggcaa tggtcgggca ccctggtgat gatcctccag
     1441 cccgccgagg agaccggcga gggcgcccgc gcgatgctcg ccgacggcct gttcacccgc
     1501 ttcccgaagc cgacccatgc cctggcctgg cacgacagcg cctcgcttcc ggcaggagtg
     1561 ctcgggatca ccgtcggtcc cgccttcgcc aatgtcgaca cggtcgacat cgcggtgaaa
     1621 ggcgtcggcg gccacggcgc ggtgccccag aacacgcgcg acccgatcgt gctggccagc
     1681 cggatcgtcg gcaccctgca gaccctggtc agccgcgagc tcgacccgca ggacgcggcg
     1741 gtggtgacgg tgggaagctt ccagtccgga accaagcaca acatcatctc cgaccaggcg
     1801 accctgctgc tgacggtgcg aagctacagc cagaagactc gccgcgcgct gctcgacggg
     1861 atccagcgca tcgcccgggg cgaggcgatc gccgccggga tgccggagga caggatgccg
     1921 gtggtgacga tccgggaggg cgagttcacg cccgcggcga tcaacacgga caaattgagc
     1981 gccgacaccg ccgctttgtt tcgcacccgc ttcggagcgg accgggtgcg cgtcgtgccg
     2041 ccgccgatgg tgggcgagga tttcggccgc taccatctcg ccgacgagaa gatcgagagc
     2101 ctgatcttct gggtcggcgg agtcccccag tcgaagtggg acgccgccgg cggcgacacg
     2161 tcgaagctgc cgtcgctgca cagcccctat tgggcgccgg aggccgagac ggtgatcgcg
     2221 acggggatc
//
LOCUS       DASWNU010000086         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_28975, whole genome shotgun sequence.
ACCESSION   DASWNU010000086 DASWNU010000000
VERSION     DASWNU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28975"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VGB57_01710"
     CDS             complement(<1..238)
                     /locus_tag="VGB57_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130091.1"
                     /translation="MRRGKNCGLLIAGGGVAGCFAALAMARLRPEAAMVLVGEEVRFG
                     GGSGMLLLDAGLSAEERDLLEPVAVRRWDSCYVSL"
     gene            385..1236
                     /locus_tag="VGB57_01715"
     CDS             385..1236
                     /locus_tag="VGB57_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY0130092.1"
                     /translation="MTVKTMWRAAAFALPLLAAAPALAQDPADEGRPYDRNSLTIGLG
                     GAYIPSYEGSDDYILTPVGLAFGKVAGFGFATRGTALYVDLIPDRRDSPVDFNFGPLA
                     SVRLDRTNRIKDPQVRALGELDTAIELGAWAGVTKNGVLHEYDSLGVRVAWQKDVTDT
                     HDSSVITPGIEYSSPLSEKTLVSFSLQGEHVGDGYARTYFSVTPAGALASGLPVYSAD
                     GGWKSWRTTLLLGQSLTGNLRNPKLSLFGGVGYSRLLGDIARSPIVRIAGDRDQYFAT
                     LGLAYTF"
     gene            1347..1724
                     /locus_tag="VGB57_01720"
     CDS             1347..1724
                     /locus_tag="VGB57_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130093.1"
                     /translation="MRGCAGLAVTGMMMVLASPAAALDRSAGGGEAKSFETVQAQSRQ
                     GALVPQNVALDSKDILPAEDPSAPRLFGSLPVAENVALGVGLFSVVGTTEKEQMRRRL
                     DPAREFLGVSGTRVAAVGMSLRF"
     gene            1879..>2688
                     /gene="dinB"
                     /locus_tag="VGB57_01725"
     CDS             1879..>2688
                     /gene="dinB"
                     /locus_tag="VGB57_01725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY0130094.1"
                     /translation="MNDAPAEGTIRKIIHVDMDAFYASVEQRDNPELRGKPLAVGGGV
                     RGVVAAASYEARKWGVRSAMPAVTARRRCPELIFVKPRFDAYRAVSQQIRAIFLDYTP
                     HVEPLSLDEAYLDVTEDLKGIGVATTIAREIRARIEAETGLTASAGVSYNKFIAKLAS
                     DQNKPDGLCVITPSRGEAFVATLPVKRFHGIGPKTAEKMARLGIETGADLKAMSLDFL
                     EHNFGSSGLYYHDLARGICHRQVRANRERKSIGAEDTFFDDLTEEAALVGEL"
BASE COUNT          453 a          952 c          857 g          426 t
ORIGIN      
        1 ggagggagac gtagcaggag tcccagcgcc tgacggcgac cggctcgagc agatcgcgct
       61 cctcggcgga gaggcccgca tcgagcagca gcatcccgct gccgccgccg aaccgcacct
      121 cctcgccgac cagcaccatc gccgcttcgg gcctgagccg cgccatggcg agcgcggcga
      181 agcacccggc gactccgccg ccggcgatca gcagcccgca atttttgccc ctgcgcatcg
      241 gcacctgctt accgcccggt tggccgcgag gccatgcccg cgtgacccac gagtcccgaa
      301 aggcccaaaa cgacacgttt ttttcatgac gatggtgaat tccttgagct agaagccggt
      361 cgtggggcaa caggagggac tttgatgacc gtgaagacca tgtggcgcgc cgccgctttc
      421 gccttgccgc ttctggccgc cgctccggcg ctggcgcagg acccggccga cgagggccgg
      481 ccttatgatc gaaacagcct cacgatcggc ctcgggggcg cctacatccc ctcctacgaa
      541 ggctcggacg attatatcct gactccggtc ggcctggcct tcggcaaggt cgccggcttc
      601 ggcttcgcca cgcgcggcac cgccctctac gtcgacctca tccccgacag gcgcgactcg
      661 ccggtggatt tcaacttcgg tccgctcgcc agcgtccgcc tcgaccggac caaccggatc
      721 aaggacccgc aggtgcgggc gctgggcgag ctcgacaccg ccatcgagct cggcgcgtgg
      781 gccggcgtca cgaagaacgg cgtcctccac gaatatgaca gcctcggagt gcgcgtggcc
      841 tggcagaagg acgtcaccga cacccacgac agctccgtca tcactcccgg catcgaatat
      901 tcctccccgc tcagcgaaaa gaccctggtc agcttcagcc tgcaaggcga gcatgtcggc
      961 gacggctacg cccgcaccta tttttccgtg accccggccg gggcgctggc gagcggactt
     1021 cccgtctatt ccgcggacgg cggctggaag agctggcgga cgaccctgct gctcggccag
     1081 tccctgaccg gcaacctcag gaaccccaag ctctcgctgt tcggcggggt cggctattcg
     1141 cggctgctcg gcgatatcgc ccgaagcccg atcgtccgca tcgccggcga ccgcgaccaa
     1201 tatttcgcga cgctgggctt ggcctatacc ttctgatttg cgtccccgtg ctccgcatgg
     1261 agaggcaaat caggatagtg acacatccgg aaccacccgg catagtcgag cgctgatatg
     1321 gggcaacagc aagaatggag cccgcgatga gaggatgtgc cgggctcgcc gtaacgggga
     1381 tgatgatggt cctcgcgtcg cctgccgcgg cgctggaccg gtcggcgggg ggcggcgagg
     1441 cgaaaagctt cgagaccgtg caggcgcaga gccggcaagg tgccttggtt ccacaaaatg
     1501 tcgctctcga ctcgaaggat atcctgcccg cggaggatcc gtccgcgccg cgcctgttcg
     1561 ggagccttcc ggtcgccgag aacgtggcct tgggagtcgg tctcttctcc gtcgtcggaa
     1621 ccaccgagaa ggagcagatg cgccgccggc tcgacccggc ccgcgagttc ctcggcgtct
     1681 ccggcacccg ggtcgccgcg gtggggatga gcctgcgctt ttagcgtcat cccggcgaaa
     1741 gccgggacct cgggcagacc cgccgagcct cattcgtctt gaggtcccgg ctttcgccgg
     1801 gatgacggca ccctccactc ctcaccccat atgcgctttg cccttttctc cgcggcccat
     1861 ccgcgctagg aacatggcgt gaacgacgcg ccggccgagg ggacgatccg caagatcatc
     1921 catgtcgaca tggatgcctt ctacgcgtcg gtcgagcagc gcgacaatcc ggagctgagg
     1981 ggcaagccgc tggccgtcgg cggcggagtc cgcggggtcg tcgcggcggc cagctacgag
     2041 gcgcgcaaat ggggcgtgcg ctcggccatg cccgccgtca ccgccaggcg gcgctgcccc
     2101 gagctcatct tcgtcaagcc gcgcttcgac gcctatcgcg ccgtctccca gcagatcagg
     2161 gccatcttcc tcgactatac gccgcacgtc gagccgctct cgctcgacga ggcctatctc
     2221 gacgtcaccg aggacctgaa ggggatcgga gtcgcgacga ccatcgcccg cgagatcagg
     2281 gcgcggatcg aggcggagac cgggctcacc gcctcggccg gagtctccta caacaagttc
     2341 atcgccaagc tagcgtcgga ccagaacaag cccgacggcc tgtgcgtgat cactccgtct
     2401 aggggagagg cgttcgtcgc caccctcccg gtcaagcgct tccacggcat cgggcccaag
     2461 accgccgaga agatggcgcg cctcggcatc gagaccggag cggacctgaa ggccatgagc
     2521 ctcgacttcc tcgagcacaa tttcggtagc tcaggccttt attatcacga cctcgcccgc
     2581 ggcatctgcc accgccaggt gcgggcgaac cgcgagcgca agtcgatcgg cgccgaggac
     2641 accttcttcg atgacctgac ggaggaggcc gccctggtcg gcgaactc
//
LOCUS       DASWNU010000087         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_3632,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000087 DASWNU010000000
VERSION     DASWNU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3632"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2734)
                     /locus_tag="VGB57_01730"
     CDS             complement(<1..>2734)
                     /locus_tag="VGB57_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0130095.1"
                     /translation="VNTQTLGDQNEPGVAALAGGGFIAYWTDTSFNSDGSSWSVRAQR
                     YDAAGARAGTEFVVNAKTVGVQQNPAAAGLPDGGFVFVYTDGIEVHAHFFEADGTRSP
                     VLVVNDPATRLQHTPAVTVLADGRVAVAWADLRDEAAGSDIRVRILNSDATIGGPEIL
                     VNATRDDIQERPQIAALGGGGFVVTWGDLSENAATGGSGIKAQVFGADGAKVGGEIVV
                     PQTVAGTQSGAFVTGLSDGGFAVAWTGADSFGRAVKGALFDSSGARNGDEFLVNDIQV
                     QDQHANFIARSAGGFSVAWADLSQQSGDVSGSGIKFTRFVASRATSDGDVIIGTEQSD
                     TIHGLGGADILEGLGGDDELFGGEEVDRLEGGLGDDRLRGDGGGDVLNGGAGRDDLAG
                     GIGNDTLNGDWDDDLLSGGDGIDYMLGGGGNDIVAGDAGDDWLNGGDGNDSLDGGAGA
                     DILEAEAGDDSLRVTATAAAARDFAHGEAGTDTLTVDLRALTVAARSQLAPVGNLAEG
                     FTCAIDSTAGHYIEWSGIEKLIVLGGSGADHFIGAAGNESFSGGSGDDRLEGGDGNDT
                     LDGEGGNDRLVGGKGDDILGVDSAGDIVVEEAGEGTDTVRASVAHTLGANLENLTGIS
                     AVGLALTGNGGNNLIIGSEGNDVIDGGAGADRMKGGNGNDIYYVDDLGDFVEEPDFFS
                     SPFGLDEIRTALPVYKMAELVWTGPVYSYVEHLTGLSTTGQSLTGNGLGNLITGNIGN
                     DFLDGDRGADRLVGGQGDDVYVVDDAGDVVVEAAGQGTDEVRVNLTRYTLGANVENAT
                     LIYAGSPNTLTGNGLNNVLRGAAGWDTLDGGAGADTLIGGAGDDWYVIDLSDTIVEAL
                     DASGGFDSVADVPFATYTLPDGVELLRGTSAQQTLIGNDGNNWLGSNGGSDTLIG"
BASE COUNT          381 a         1002 c          879 g          472 t
ORIGIN      
        1 cgccgatcaa ggtgtcgctg ccgccgttgg accccagcca gttgttgccg tcattgccga
       61 taagggtctg ctgggcgctc gtgccgcgga ggagctcgac cccgtccgga agcgtgtagg
      121 tcgcgaacgg cacatccgcc acggagtcga agccgcccga ggcgtccagc gcctcgacga
      181 tcgtgtcgga gaggtcgatc acgtaccagt cgtcgccggc gccgccgatc agggtgtccg
      241 cgcccgcgcc gccgtccaga gtgtcccacc cggcggcgcc tcgaagcacg ttgttcagcc
      301 cgtttccggt cagggtgttg ggcgaaccgg catagatgag ggtcgcattc tcgacgttgg
      361 cgccaagcgt gtaccgggta agattgaccc ggacctcgtc cgtgccctgg cctgccgctt
      421 ccaccaccac gtcgccggcg tcgtcgacca cgtagacatc gtcgccctgg cccccgacca
      481 gcctgtcggc gccgcggtcg ccgtcgagga agtcgttgcc gatattgccg gtgatgaggt
      541 tgccgaggcc gttgccggtc agcgactggc cggtcgtgga caggccggtc agatgctcga
      601 catagctgta gaccgggccg gtccagacca gctcggccat cttgtagacg ggcagggcgg
      661 tccggatctc gtcgagcccg aacggcgagc tgaagaagtc gggttcctcg acgaaatcgc
      721 cgagatcgtc gacataatag atgtcgttgc cgttgccgcc cttcatccgg tcggcgcccg
      781 cgccgccgtc gatgacgtcg ttgccctcgc tcccgatgat gaggttgttg cctccgttgc
      841 cggtcagcgc cagccccacg gcgcttatcc cggtcaaatt ctcgaggttc gcgccgagcg
      901 tgtgggcgac gctggcccgg accgtgtccg tcccttcgcc cgcttcctcg acgactatgt
      961 cgccggcgga atcgacgccg aggatgtcgt cgcccttgcc gccgaccagc ctgtcgttgc
     1021 cgccctcgcc gtcgagcgtg tcgttgccgt cgccgccttc cagccggtcg tcgcccgagc
     1081 cgccgctgaa gctttcattg cccgcggcgc cgatgaaatg gtccgcgccc gagccgccga
     1141 gcacgatcag cttctcgatc ccgctccact cgatatagtg cccggcggtc gagtcgatag
     1201 cgcatgtgaa gccctccgcc agattgccca ccggggccag ctggcttcgc gccgccaccg
     1261 tgagcgcccg aagatcgacg gttaaagtgt ccgtgccggc ctcgccatgc gcgaagtccc
     1321 gggccgcggc cgcggtcgcg gtcacccgga gcgaatcgtc gcccgcctcg gcttcgagta
     1381 tgtccgcgcc cgcgcctccg tccagcgagt cgtttccgtc gccgccgttg agccagtcgt
     1441 cgccggcatc gccggcgacg atgtcgttgc cgccgcctcc cagcatgtag tcgatgccgt
     1501 cgccgcccga gagcaggtcg tcgtcccaat cgccgttgag cgtgtcgtta ccgattccgc
     1561 cggccagatc gtctcgccct gcgccgccgt tcagaacgtc cccgccgccg tcgccccgaa
     1621 gccgatcgtc gccgagtccg ccctcgagcc ggtccacctc ctcgccgccg aacagctcgt
     1681 cgtcgccgcc gagcccttcc aggatatcgg ccccgccgag gccgtggatc gtgtccgact
     1741 gctcggttcc gatgatcacg tcaccgtcgg atgtcgcccg gctggcaacg aagcgggtga
     1801 acttgatccc cgatccgctc acatccccgc tctgctggct gagatccgcc caggcgacac
     1861 tgaagccgcc ggccgaacgc gcgatgaagt tcgcatgctg gtcctggacc tggatgtcgt
     1921 tgaccaggaa ctcgtcgccg ttgcgggcgc cgctcgaatc gaacaacgcc cccttcaccg
     1981 cccggccgaa gctgtcggcg ccggtccacg cgacggcgaa gccgccgtcg gaaagtcccg
     2041 tcacgaaggc gccgctctgg gtccccgcga cggtctgcgg aaccacgatc tcaccgccga
     2101 ccttcgctcc gtcggcaccg aagacctgcg ccttgatgcc cgatccgccg gtcgccgcgt
     2161 tctcgctgag gtcgccccag gtcacaacga acccgccgcc gccgagagcc gcgatctgcg
     2221 gccgctcctg gatgtcgtcc cgggtcgcgt tgaccaggat ttcgggaccg ccgatcgtgg
     2281 cgtccgaatt gaggatccgg actcgtatgt cgctgcccgc cgcttcgtcg cgaagatccg
     2341 cccaggcgac cgcgactcgc ccgtccgcca gcaccgtgac cgcgggggtg tgctgaagcc
     2401 gggtcgccgg gtcgttcacc accagcacgg ggctcctggt cccgtcggct tcgaagaaat
     2461 gcgcgtggac ctcgatcccg tcggtataga cgaagacgaa gccgccgtcc ggaaggcccg
     2521 cggcggccgg attctgctgc actccgaccg tcttggcgtt gacgacgaac tcggtgccgg
     2581 cgcgggcgcc ggcggcatcg tacctctggg cgcgaacgct ccaggagctg ccgtcgctgt
     2641 tgaagctggt atccgtccaa taagcgatga agccgccgcc ggcgagcgcc gcgactcccg
     2701 gctcattctg gtccccgagg gtctgagtgt tgac
//
LOCUS       DASWNU010000088         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_496503, whole genome shotgun sequence.
ACCESSION   DASWNU010000088 DASWNU010000000
VERSION     DASWNU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496503"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1821
                     /locus_tag="VGB57_01735"
     CDS             220..1821
                     /locus_tag="VGB57_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0130096.1"
                     /translation="MGAVTAHIDTFVRDRLPPPEAQPEFLFDLPELRYPERLNAAVEL
                     IDKGEPEALAVINDNGRWTYGEMRDLSDRIARLLAEEEDLVPGDRVLLRGPNGAMLFA
                     CWLGVLKAGGIAVATMPVLRSGEVATIVERAQVHHAIVDDRFLDDFMKAADETGLIRS
                     LLTYHGDSGAGRLERRLESVRPGFEAVETGRDDVALIAFTSGTTGRPKGCVHYHRDIL
                     APADSFARHVLKPSPGGRWACSAPIAFTFGLGMLLIFPWRFGGAAVTIEQPGPRPLLD
                     AIARFEVTTLATAPTAYKAMLGLLGEHDVSSLATCVSAGEHLPAATWQAWRDATGLRI
                     VDGIGATEMMHIFISASGDDIRPGATGKAVPGYRACVLDGEGRPLPHGTGRLAVKGPT
                     GCRYFDDERQADYVQGGWNVTGDTYRADEQGYFWYVARSDDMIVSAGYNIAAPEVENA
                     LYAHPAVQECAVIGLPCEERGQKVKAFVVAAPGHAAGAELAKALQDHAKAVIAPYKYP
                     RELEFVEALPKTATGKLQRFALRRG"
     gene            complement(2039..>3226)
                     /locus_tag="VGB57_01740"
     CDS             complement(2039..>3226)
                     /locus_tag="VGB57_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130097.1"
                     /translation="VHILVMLNAQMTDRIEPVPELAALCAEIEAICAQSNGGVTLRAG
                     HRGADEKWQHLAALKDKEGLPCPKEHFGYTDGISDPVFLGQYPNGEEQARSVGSGATD
                     GEGKWRPLATGEFLLGWPDEAQETAGAAMPLDFSRNGTFFAYRKLHQDIESFYGWVDE
                     SARQLQTLWKLQTFDIAKEILLAKMAGRWSDGVPLMEAPDYRAWEEFNRKYPPDELHR
                     TRNDTERARRLVDFAYRDDLDGLKCPATAHIRRTNTRDMLDPRATAASPKSRMGSTLN
                     NRRRLLRRGLPYGSAADPKGEHGIIMLAVCSSLQRQFEFVQQQWINYGLDSNAGNDTD
                     PLIGNHPPDSKFLIPADPGSGNPPFIATRLRQWVQTRGGGYFFMPSMTALRMIGMGVV
                     DPT"
BASE COUNT          498 a         1108 c         1090 g          530 t
ORIGIN      
        1 aggaggtgat aggacgccac taccccgcga tggcggtggt cgaggtaggg gccctggacg
       61 agcccggcgc ccgcatcgag atcgagacga tcgcggtggt gcccgaatgg tgacagtcac
      121 cgtcatcgcg agcgaggcga agcaatccag cacggcgcct gacacgagcg ccgcgctgga
      181 ttgccgcgtc gcttcgctcc acgcaatgac ggtgggcggg tgggggcagt gaccgcccac
      241 atcgacacct tcgtccgcga ccgcctccct ccgccggagg cgcagccgga gttcctgttc
      301 gacctgccgg agctgcgata tcccgaacgg ctgaacgccg cggtcgagct gatcgacaaa
      361 ggcgaacccg aagccctcgc cgtgatcaac gataacggcc gctggaccta tggcgagatg
      421 cgggacctca gcgatcgcat cgcccgcctg ctcgccgaag aggaggatct cgtccccggc
      481 gaccgggtgc tgctgcgcgg tccgaacggg gcgatgctgt tcgcctgctg gctgggcgtt
      541 ctcaaggccg gcgggatcgc cgtcgcgacc atgccggtcc tgcgctccgg cgaggtggcg
      601 acgatcgtcg agcgcgcgca ggtccaccac gcgatcgtcg acgaccgctt cctcgacgat
      661 ttcatgaagg cggcggacga aaccggcctg atccgctcgc tcctcaccta ccacggcgat
      721 tccggcgccg gccggctcga gcggcggctg gaatcggtgc gccccggctt cgaagcggtc
      781 gagaccgggc gggacgacgt cgccctgatc gccttcacct ccgggacgac tggccgcccc
      841 aaaggctgcg tccattatca tcgcgacata ctcgcccccg ccgacagctt cgcccgccat
      901 gtgctgaagc cctcacccgg cggccgctgg gcctgctcgg cgccgatcgc cttcaccttc
      961 gggctgggca tgctcctcat cttcccctgg cgtttcggcg gcgcggcggt gacgatcgag
     1021 cagccggggc ccaggccgct gctcgacgcg atcgcccggt tcgaagtcac cacgctcgcc
     1081 accgccccca ccgcctacaa ggcgatgctc ggcctgctcg gcgagcacga cgtctcctcc
     1141 ctcgccacct gcgtctcggc gggcgagcac cttcccgccg ccacctggca ggcctggcgc
     1201 gacgccaccg gtctccgcat cgtcgacggc atcggagcga ccgagatgat gcatatcttc
     1261 atctcagcct ccggcgacga catccgcccc ggcgccaccg gcaaggcggt tccgggctac
     1321 cgcgcctgcg ttctcgacgg cgaggggcgg ccccttcccc acggcaccgg ccgcctggcg
     1381 gtcaagggcc cgaccggctg ccgctatttc gacgacgagc gccaggcgga ttacgtccag
     1441 ggtggctgga acgtcaccgg cgacacctac cgggccgacg agcagggcta tttctggtac
     1501 gtcgcccgct ccgacgacat gatcgtgtcg gccggctaca atatcgccgc gccggaggtc
     1561 gagaacgcgc tctacgccca tcccgccgtt caggaatgcg ccgtcatcgg cctaccctgc
     1621 gaggagcggg ggcaaaaagt gaaggccttc gtcgtggcgg cgccgggaca cgccgcgggc
     1681 gcggagctgg caaaggcgct ccaggaccat gcgaaggctg tcatcgcgcc gtacaaatat
     1741 ccccgcgagc tggagttcgt ggaggcgctg ccgaagacgg cgaccgggaa gctgcagcgc
     1801 ttcgcgttgc ggcgaggcta ggctgtatcg accttcagcg agccaggttt ctaaaatcct
     1861 cccctgcatt tgcaggggag ggggaccatg cgaagcatgg tggaggggct tcttgctgcc
     1921 tccggaagaa cccctccacc gcgcttcgcg cggtccccct cccccggaaa tccgggggag
     1981 gacttaggac gacttagctg aatgtgatcc agcctgatcc cgaagctcta cccgggcctc
     2041 aggtcggatc gaccacgccc atgccgatca tcctgagcgc cgtcatcgac ggcatgaaga
     2101 aatagccgcc gccgcgggtc tggacccatt gcctcagccg agtggcgatg aagggcggat
     2161 tgcccgatcc cggatcggcc gggataagga acttcgaatc cggtgggtga ttgccgatca
     2221 gcggatccgt gtcgttgccg gcgttggaat ccagcccgta gttgatccac tgctgctgca
     2281 cgaactcgaa ctggcgctgc aggcttgagc agacggcgag catgatgatg ccgtgctccc
     2341 ccttggggtc ggccgcgctt ccatagggca ggccgcggcg gagcaggcgc cgccggttgt
     2401 tgagggtcga gcccatgcgc gatttgggcg aggccgcggt ggctcgcggg tcgagcatgt
     2461 cgcgcgtatt ggttcggcgg atgtgcgcgg tcgccgggca tttgaggccg tcaaggtcgt
     2521 cgcgataggc gaagtcgacg aggcgccggg cccgctcggt atcgttgcgc gtgcgatgga
     2581 gttcgtcggg cgggtatttg cggttgaact cctcccaggc gcgatagtcg ggcgcctcca
     2641 tcaacggaac gccgtcgctc cagcggccgg ccatcttcgc gagcagaatc tccttcgcga
     2701 tgtcgaacgt ctggagcttc cagagcgtct gaagctgcct ggcggattcg tccacccagc
     2761 cgtagaagct ttctatgtcc tggtggagct tccggtaggc gaagaaggtg ccgttgcggc
     2821 tgaaatcgag cggcatcgcg gcaccggcgg tctcctgcgc ctcgtcaggc cagccgagca
     2881 ggaactcgcc ggtggcgagc ggccgccatt tcccctcgcc gtcggtcgcg cccgagccga
     2941 ccgagcgcgc ctgctcctcc ccgttcggat actggccgag gaagaccggg tcgctgatcc
     3001 cgtcggtata gccgaaatgc tccttcgggc agggcaggcc ctccttgtcc ttgagcgcgg
     3061 cgagatgctg ccatttctcg tcggcgccgc gatggcccgc gcgcagggtc actccgccgt
     3121 tcgactgcgc gcagatcgcc tcgatttcgg cgcacagggc ggcgagctcg ggcaccggct
     3181 cgatccgatc cgtcatctgg gcgttgagca tgaccaggat gtggac
//
LOCUS       DASWNU010000089         5996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_55326, whole genome shotgun sequence.
ACCESSION   DASWNU010000089 DASWNU010000000
VERSION     DASWNU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55326"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..265
                     /locus_tag="VGB57_01745"
     tRNA            191..265
                     /locus_tag="VGB57_01745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:223..225,aa:Gly,seq:gcc)
     gene            305..763
                     /locus_tag="VGB57_01750"
     CDS             305..763
                     /locus_tag="VGB57_01750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0130098.1"
                     /translation="MISLALLLAAAPAPAPKPVPPRSFIPPVSVRAVVTTASGLRFET
                     LRAGAGARPSAADQVLVTSEGHLADGTLFDAGPQPTAFPVAGVVPGFAEALQLMQKGG
                     RYRFWIPPALAYGPAGVPGAIPPNAELEFIVTLIDMRPAAAAAEPAPTQP"
     gene            complement(768..1016)
                     /locus_tag="VGB57_01755"
     CDS             complement(768..1016)
                     /locus_tag="VGB57_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130099.1"
                     /translation="MSDPAPSFANDIAPMFSDYQAQMAWRFDLTSYDDVKANARIIYN
                     VIYWDKNGGPQMPPGPFPPFPSDFVQLFANWIDTGYGA"
     gene            complement(1013..2311)
                     /locus_tag="VGB57_01760"
     CDS             complement(1013..2311)
                     /locus_tag="VGB57_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130100.1"
                     /translation="MARGSNDPVPRQLLAEGLDFPTSIALGGDGALYVAESGLPFGGA
                     APGGRIVRIGRDGAKEVVLDGLRAPVNGLTEAEGDFYIAEGGNPGRISRWRPGGDRET
                     VLDGLPAGGNYHTNMVAVGRDGWLYFGQGAMTNSGVVGLDAIDLAWLRRLQHPVDLPG
                     YPVTVSGRSFETRDPRSDDPAARASTAGFAPFGETRSGDERLEARLPCTAAVMRCRPD
                     GGDLELVAWGLRNPFGLGFLPDGRLIATDQGADDRGSRPIGAGPDLLYEVKAGAWYGW
                     PDFVGGVPVTDPRFTPQRGDPPGFLLADHASLPPPERPLLEFAVNAAATKFAVLPDSA
                     PWPGQLIVAIFGDEKPMTGPAGPKVGRSLLRVDPGGWCGHAFDVGRFDRPIDVAVDPD
                     GKSLLVLDFGHFEMLEAGGLDAVAGSGRLWKIELNQGDSR"
     gene            complement(2311..2814)
                     /locus_tag="VGB57_01765"
     CDS             complement(2311..2814)
                     /locus_tag="VGB57_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130101.1"
                     /translation="MTLFGFGILYGVDNAAVALVLAPLLGWRRGLALAALFGLAEALM
                     PLAGLALAWPPAVAAESAVRAGILALAATSLAGLLLVRRDPAAAIRSPGAMVALALLL
                     SLDNVVAGGEAGSVAGAAAIGLASASLAAMGCALGALLGARLSPSAAAVGSTGGLVVL
                     ALANAVS"
     gene            complement(2811..4595)
                     /locus_tag="VGB57_01770"
     CDS             complement(2811..4595)
                     /locus_tag="VGB57_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0130102.1"
                     /translation="MTDPTGWWMYHGDQAHSGYVSGGAINSALLRKPSGFGTLLSLNV
                     GGPILSVPAVCNGFIYVGLANSRDAIGALGGRLLKIDITNGKTVAHYAWQILPAERDS
                     HGFCGMGSTPTVVGGFVYFVAFNAKLYCLKESDLSEVWVTDLRNRDLSKNQPIQNYFP
                     YPTLDVGNYPPAAGWSAPLFVNGRLILGIGEGENPYVFSFVYCIDAASGHVVWVMCTN
                     QMHADAVNVPNLIPLATAPDEIPAGFTVGPNPAFPGSPSLLPPSGQAYTGASVWGCIA
                     YDETLDTIYCPTGNGNPDGPLPTPGWTNGLLALDASTGAFKAFFQPGPETNYRPTDID
                     VDMGGSPTLLTLDDGRRAVGVGSKNGTYFLLDAATLELIDQRQMLPTYNNGAPIQDVD
                     PIDGTGDAENYSGTYSTAAVDPVARRIFCGVGGNNYHGLPGIDYKSTPFMRVMDYQLN
                     DAWPLDTNDPPRYAKAMTGTMNTIVDGQVQANPINAMYQNAVDSGLSSPAVVNDVVFM
                     ATTGVNLYAFDVADGTPLWYDQLGSQTGGMSGGYGYCMGPAVWGDYVVAGGLITGGDG
                     GILRIYGPLPPPPPPPPPPPPPSAGGQA"
     gene            complement(4592..>5996)
                     /locus_tag="VGB57_01775"
     CDS             complement(4592..>5996)
                     /locus_tag="VGB57_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130103.1"
                     /translation="PEPGPADQREKAVGTAQPTSGATLSGIMPLDVQLVLNPGIGPND
                     QVRIRPNFDDFSWQSFVALAWPVDPALRGSPRNPTDPNTLLGAGNAYASVWSSYRDAN
                     DLYPGAKPDRPVPFDAPATSGCGSGAGLVLVKNSKMGDPLSDTQQAFSLPLVDQSRNY
                     VYYQMLFNRDQYDFVRGSDSDPTTWLYRKTALFNAEKVAPIGMPQANADTKVQGAMML
                     KAAWKQLTKQDDASRYYTRQAQIQDPATGKCGAPTTVGLIGLHIAHKLSDFPQWVWSS
                     FEHVDNVPPASGPAPRRMTLNNGTNIPPVNGDGFANAPASSTPVQDPIPVQVVRMNPI
                     PTTPAKVSGQQYPSGAGSTADLNAAYQALLKGSVWANYQLVITQWPFNAVPQNRFKPP
                     EKQGVYPGDAGGAFPLYGAVNTSMETYFQTQRAALGAGGNSCMQCHYQAGMTDFSWSL
                     KLRSQNPKTTAQQRGRP"
BASE COUNT          936 a         1988 c         2105 g          967 t
ORIGIN      
        1 ctatgtggga ttgcaccgcc tcctgccaac cctatcgttt cccggcgaag gccgggaccc
       61 agtccggcct cagcgactgg accccggcct tcgccgggga gcaatagccc ccttgccggg
      121 tggcggaccc catgctagca gccgccgcct tacctcggcc gggacgcaac gcggaccggc
      181 gcggctcaga gcgggcgtag ctcaggggta gagcacaacc ttgccaaggt tggggtcgag
      241 ggttcgaatc ccttcgcccg ctccagtcat tttccgccgg cgggacgggc ggccgggagt
      301 ccgaatgatc agccttgccc ttctcctcgc cgccgcgccg gccccggccc cgaagccggt
      361 tccgccccgc tccttcatcc cgccggtatc cgtccgagcc gtcgtcacca cggcctccgg
      421 gctgcgcttc gagaccctga gggccggcgc cggcgccagg ccgagcgccg ccgatcaggt
      481 gctcgtcacc tctgagggcc atctcgccga cgggacgttg ttcgatgccg gcccgcaacc
      541 gaccgccttt ccagtggcgg gcgtggtgcc cggcttcgcc gaagcgctgc agctgatgca
      601 gaagggaggg cgctaccgtt tctggatccc gccggcgctc gcttacgggc ccgccggcgt
      661 tcccggcgcc attccgccca atgccgagct cgagttcatc gtcactctta tcgacatgcg
      721 gccggccgcg gccgccgcgg agccggctcc tacccagccc tgaggcgtca ggcgccgtag
      781 ccggtgtcga tccagttggc gaagagctgg acgaagtccg atggaaaggg cgggaacggc
      841 cccggcggca tctggggacc gccgttcttg tcccaataga tgacgttgta gatgatccgg
      901 gcgttcgctt tgacgtcgtc ataggacgtg agatcgaacc gccacgccat ctgcgcctga
      961 tagtcgctga acatcggagc gatgtcgttg gcgaaggacg gcgctggatc gctcatcgcg
     1021 aatctccctg gttcaattcg atcttccaca gccggcccga tcccgcgacg gcgtcgaggc
     1081 cgccggcttc cagcatctcg aaatggccga agtcgagcac gagcagcgac tttccatcgg
     1141 gatcgacggc gacgtcgatc ggccggtcga agcggccgac gtcgaacgcg tggccgcacc
     1201 agccgccggg atcgacccga agcaggctcc ggccaacctt gggccccgcc ggccccgtca
     1261 tcggcttctc gtcgccgaag atcgcgacga tcagctgtcc gggccaggga gcggaatcgg
     1321 ggaggaccgc gaatttggtg gcggccgcgt tgaccgcgaa ttcgagcagc ggccgctccg
     1381 gcggcgggag cgaagcgtga tcggcgagca ggaagcccgg cggatcgccg cgctgcgggg
     1441 taaaccgcgg gtccgtgacc ggcactccgc cgacgaagtc gggccagccg taccaggcgc
     1501 cggccttgac ctcgtagagg aggtccggtc cggcgccgat gggccgggag ccgcggtcgt
     1561 ccgccccctg atcggtggcg atcagcctcc cgtccgggag gaagccgagg ccgaagggat
     1621 tgcgcaggcc ccaggcgacc agctcaagat cgccgccgtc gggccggcat cgcatcaccg
     1681 ccgccgtgca gggcaggcgc gcctcgaggc gctcatcgcc gctccgagtc tcgccgaagg
     1741 gcgcgaagcc ggctgtggag gcgcgcgccg cgggatcgtc gctccggggg tcgcgggtct
     1801 cgaagcttcg gcccgaaacc gtgaccgggt agccgggaag gtcgacgggg tgctgaagcc
     1861 ggcgcagcca ggcgagatcg atggcgtcca ggccgaccac gcctgaattg gtcatcgccc
     1921 cctggccgaa atagagccag ccgtcccgcc ccaccgccac catgttggtg tggtaatttc
     1981 ctcccgccgg aaggccgtcg agaaccgtct cgcgatcgcc gccgggccgc cagcggctga
     2041 tccggccggg atttccgccc tcggcgatgt agaagtcgcc ctccgcctcg gtcagcccgt
     2101 tgaccggcgc ccgcagcccg tcgagcacga cctccttcgc cccgtccctg ccgatccgga
     2161 cgatccggcc gcccggagcc gcgccgccga agggcagccc gctctccgcg acgtagagcg
     2221 cgccgtcgcc gccgagggcg atgctggtcg ggaagtcgag gccttcggcg agcagctgcc
     2281 gggggaccgg gtcgttcgag ccccgcgcca tcagctcaca gcgttcgcca gcgccagcac
     2341 gaccagcccg cccgtggagc cgacggcggc ggcactcggc gacaaccgag cgcccagcag
     2401 cgccccgagg gcgcagccca ttgcggcaag gctcgcgctc gcaaggccga tggccgcggc
     2461 gcccgcgact gagccggcct cgccgccggc gacgacattg tcgaggctga ggagcagggc
     2521 gagcgcgacc atcgcaccgg gcgagcggat cgcggcggcc gggtcgcggc ggacgagcag
     2581 gagccccgcc aggctggtcg cggccagagc gaggatcccg gcccgaacgg ccgattccgc
     2641 ggccacggcc ggaggccagg ccagagcgag ccccgccagc ggcatcagcg cctcggcgag
     2701 cccgaacagg gcggccaagg cgagtccccg ccgccagccg agcagcggcg ccagcacgag
     2761 cgccacggcg gcattatcga cgccgtacag gatcccaaag ccgaataagg tcatgcctgt
     2821 cctcccgcgg atggtggtgg gggcggtggt ggtggcggcg gcggtggcgg gagcggtcca
     2881 tagatcctca atatcccgcc gtctccaccg gtgatcagcc cgccggcgac gacatagtcg
     2941 ccccagaccg ccgggcccat gcaatagccg tagccgccgc tcatgccgcc ggtctgcgac
     3001 ccgagctggt cgtaccagag cggagtcccg tcggcgacgt cgaaggcgta gaggttgacc
     3061 ccggtggtgg ccatgaacac cacgtcgttg acgacggccg gcgagctgag acccgaatcc
     3121 accgcattct ggtacatggc gttgatcgga ttcgcctgga cctgaccgtc gacgatcgtg
     3181 ttcatcgtgc cggtcatggc cttggcgtaa cgcggcggat cgttggtatc gagcggccac
     3241 gcatcgttca gctggtagtc catcacccgc atgaacggcg tgcttttgta gtcgatgccg
     3301 ggcagaccat gataattgtt gcccccgacg ccgcagaata ttcgcctcgc gacggggtcg
     3361 acggctgccg tgctgtacgt cccggaataa ttttctgcat ctcccgtgcc gtcgatcggg
     3421 tcgacgtcct gaatcggcgc gccattattg tatgtgggca gcatctgacg ctggtcgatg
     3481 agttccagcg ttgcggcgtc gagcaggaaa taggtgccgt tcttcgaccc gacgccgacg
     3541 gcgcggcgcc cgtcgtccag cgtcagcagg gtcggtgacc cgcccatgtc gacgtcgatg
     3601 tcggtcggcc ggtaattggt ttccggtccc ggttggaaaa aggccttgaa ggcgcctgtg
     3661 gacgcatcga gcgcgagaag cccgttcgtc caccccgggg tcggcaaagg cccgtcgggg
     3721 ttgccgttgc cggtcgggca atagatcgtg tcgagcgttt cgtcataggc gatgcagccc
     3781 catacggagg cgccggtata ggcctggccg ctgggcggca gcaacgaagg cgaccccgga
     3841 aaggccgggt tcggtccgac ggtgaacccg gcgggaattt cgtcgggggc ggttgccagg
     3901 ggaatcaggt tggggacgtt gaccgcgtcc gcgtgcatct gattggtgca catgacccag
     3961 acgacatggc cgctggccgc atcgatgcaa tagacgaagc tgaagacgta ggggttttcg
     4021 ccctcgccga tacccaggat cagccgcccg ttgacgaaca gcggcgccga ccagccggca
     4081 gcgggcggat aattcccgac atcgagggtc gggtagggga agtaattctg gatcggctgg
     4141 ttcttggaca ggtcccggtt ccgcaggtcc gtgacccaga cctcggaaag atccgattcc
     4201 ttcaggcagt agagcttggc gttgaacgcc acgaagtaga cgaagccgcc gacaaccgtc
     4261 ggagtcgagc ccatgccgca gaagccatgg ctgtcccgct cggcggggag tatctgccag
     4321 gcgtagtggg cgaccgtttt gccgttggtg atgtcgatct tgagcaaccg gccgcccaat
     4381 gcgccgatcg cgtcgcgcga gttggcgagc ccgacataga tgaagccgtt gcaaaccgcc
     4441 ggcaccgaca ggatcggccc gccgacgttg agggagagga gcgtcccgaa cccgctgggc
     4501 ttcctcagca aggcggagtt gatcgcgccg ccggagacgt agccgctatg cgcctggtcg
     4561 ccgtgataca tccaccatcc ggtcgggtca gtcatggccg tcctcgctgc tgagcggtgg
     4621 tcttcgggtt ctgcgaccgc aacttcagcg accagctgaa atcggtcatg cccgcttggt
     4681 aatggcattg catgcagctg tttccgccag cgccgagcgc ggcgcgctgg gtctggaaat
     4741 aggtttccat gctcgtattg accgcgccgt agagcggaaa ggcgccgccc gcgtcgccgg
     4801 gatagacgcc ctgcttctcg ggcggcttga accggttctg cggcaccgcg ttgaacggcc
     4861 actgggtgat cacgagctgg taattggccc agacgctgcc cttgagcagc gcctggtagg
     4921 cggcgttgag gtcggcggtc gacccggcgc cgctgggata ttgctggccg gataccttgg
     4981 ccggagtggt cgggattgga ttcatccgga cgacctgcac tggaatgggg tcttgcaccg
     5041 gcgtcgagct ggccggcgca ttcgcgaagc cgtcgccgtt gacgggcggg atgttcgtgc
     5101 cgttgttgag agtcatgcgc ctgggcgccg ggccggaggc cggaggcacg ttgtcgacat
     5161 gctcgaagct cgaccagacc cattgcggga agtcgctgag cttgtgggcg atgtggagcc
     5221 cgatgaggcc gacggtcgtc ggggcgccgc acttcccggt cgccggatcc tggatctggg
     5281 cctggcgggt gtaatagcgg ctggcatcgt cctgcttggt cagctgcttc caggccgcct
     5341 tcagcatcat cgcgccctgg accttggtgt cggcgttggc ctgcggcatt ccgatcggcg
     5401 ccaccttctc cgcattgaac agcgcggtct tgcggtagag ccaggtcgtg gggtcggagt
     5461 cgctgcccct gacgaaatca tattggtccc ggttgaagag catctggtag tagacgtaat
     5521 tgcgggactg gtcgacgagc ggaagcgaaa aggcctgctg cgtgtcgctc agcggatcgc
     5581 ccatcttgct gttcttgacg aggacgagcc cggccccgga tccgcagccg gaggtggccg
     5641 gggcgtcgaa cggcaccggc ctgtcgggct tggcgccggg atagagatcg ttggcgtcgc
     5701 ggtagctgct ccacacggaa gcataagcgt tgccggcccc gagcaaggtg ttcggatcgg
     5761 tcggattgcg cgggctgccc cgcaaggcgg ggtcgaccgg ccaggcgagg gcgacgaagc
     5821 tctgccagga gaaatcgtcg aagttcgggc ggatcctcac ctgatcgttc gggccgatcc
     5881 cgggattgag caccagctgc acgtcgagcg gcatgatgcc cgacagagtg gcgccggacg
     5941 tgggctgcgc cgtgccaacc gctttttccc gttgatctgc cggtccgggc tccggg
//
LOCUS       DASWNU010000090         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_288953, whole genome shotgun sequence.
ACCESSION   DASWNU010000090 DASWNU010000000
VERSION     DASWNU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288953"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VGB57_01780"
     CDS             complement(<1..1267)
                     /locus_tag="VGB57_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0130104.1"
                     /translation="MRRIRGMVLIVVLLGLALPGSAGAAERVIPPTKANAKTLVFPLQ
                     LPAEASVRGAWVEGRAGRLDLPRAAVVRGLARRQLKVRLSPARRDRIGSNPKLVVEVD
                     LTGEPGAPAPAPAPAPAPAPVPDPAPAPAGPVPPPVDVLPAIPLEPSPVSDDCTRYAS
                     PAGSDSGDGSRATPFRSSQRLVDSLGPGDVGCLDAGTYDQHILAIRQGGTAEERVVLR
                     STPGQRALVTGQIWVAEGADYVTVALIDHDATHAPSDPRPSPVVNGDMALFYGMDVWS
                     DNGVCFYLGDREWGVAHGTAIRGNRIHDCGEPGMNRRHGIYIAQAVGTVIEDNAIYNN
                     PDRGIQFYPNADETIVRHNAIVGNGEGIIFSGDYGDASSGNVVEENVIANSRLRHDVE
                     AWWPEGNPIGEGNVVRRNCISGGNFGEIEE"
     gene            complement(1325..>1672)
                     /locus_tag="VGB57_01785"
     CDS             complement(1325..>1672)
                     /locus_tag="VGB57_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130105.1"
                     /translation="DAGRDAAREQLLIDFAFLIAYGAFLTLAVAAVRDRLEGQGRHRL
                     AAIGAGVVAFGAAAAAFDAAENLCLLAVLGEGGQAFPVLASIFAATKFALLLGAIAYL
                     LVGIGLRPRNRFR"
BASE COUNT          241 a          552 c          636 g          243 t
ORIGIN      
        1 gttcttcgat ctccccgaag ttgcccccgg agatgcagtt gcggcgcacc acgttgcctt
       61 ccccgatcgg gttgccctcc ggccaccagg cctcgacgtc gtgccgcagc cgggagttgg
      121 cgatcacgtt ttcctcgacg acgttgccgc tggaggcgtc gccgtagtcc ccggagaaga
      181 tgattccctc gccgttgccg acgatcgcgt tgtggcggac gatcgtctcg tcggcgttgg
      241 ggtagaactg gatgccgcgg tcggggttgt tgtagatcgc gttgtcttcg atcaccgtgc
      301 ccactgcctg ggcgatgtag atcccgtgcc gccggttcat ccctggttcg ccgcagtcgt
      361 ggatccggtt gccgcggatg gcggtcccgt gggcgacccc ccactcgcgg tcgccgaggt
      421 agaagcagac cccgttgtcg ctccagacgt ccatcccgta gaacagcgcc atgtcgccgt
      481 tgacgaccgg agagggccgg ggatccgacg gcgcgtgggt cgcgtcgtgg tcgatcaggg
      541 cgacggtcac gtagtcggcg ccctcggcga cccagatctg gccggtgacc agggcccgct
      601 gtccgggggt ggagcgcagg acgacccgtt cctcggcggt gccgccttgg cggatcgcga
      661 ggatgtgctg gtcatacgtc ccggcgtcga ggcatccgac gtcgcccggg ccgagggagt
      721 cgaccagccg ctgggagctg cggaacggcg tcgcccgcga gccgtcgccg ctgtcggatc
      781 ccgccggcga ggcgtagcgg gtgcagtcgt cggaaaccgg gctcggctcg agggggatcg
      841 cgggcaggac gtcgaccgga ggaggcacgg ggccggcggg agccggagcc gggtcgggaa
      901 ccggagccgg ggcgggagcg ggcgcaggag ccggagccgg ggcgccgggc tcgcccgtga
      961 ggtcgacctc gacgacgagc ttcgggttgc ttccgatccg atcccgtcgg gcgggcgaaa
     1021 gccggacctt cagctggcgc cgcgcgaggc cgcggacgac ggcggcgcgg ggcaggtcga
     1081 ggcgtcccgc ccggccttcg acccaggcgc cgcgaacgct ggcctcggcc gggagctgga
     1141 ggggaaatac aagcgtcttg gcattcgcct tcgtgggcgg gatgacccgc tcggccgccc
     1201 cggcagagcc gggaagggca aggcccagga ggaccacgat gaggaccatg cctcggatgc
     1261 gacgcatgga gtccctatcg accccgatcc ccgactcata gctttagcag ggatatctgg
     1321 acggttatcg gaaccgattg cgcggcctca gcccgattcc gacgaggaga taggcgatcg
     1381 ccccgagcag gagggcgaac ttggtcgcgg cgaagatcga ggccaggacc gggaaggctt
     1441 gtccgccttc gccgaggacc gcgaggaggc agaggttctc ggccgcgtcg aaggcggcag
     1501 cggcggcccc gaacgcgacc accccggccc cgatcgcagc caggcggtgc cggccctgcc
     1561 cctccaggcg gtcgcggacc gcggcgacgg cgagggtgag gaacgccccg tacgcgatca
     1621 ggaaggcgaa gtcgatcaac agctgttccc gggctgcgtc ccggccggcg tc
//
LOCUS       DASWNU010000091         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_211695, whole genome shotgun sequence.
ACCESSION   DASWNU010000091 DASWNU010000000
VERSION     DASWNU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211695"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..>1527)
                     /locus_tag="VGB57_01790"
     CDS             complement(215..>1527)
                     /locus_tag="VGB57_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY0130106.1"
                     /translation="VIHHYWSASEVAEAGVANPVDASPEEGVDELEKLLFSAVGKQMM
                     SDVPLGAFLSGGVDSSTIVALMQAQSSRPVKSFSIGFEDPAYNEAEHAGAVARHLGTD
                     HTELYVRAEDALVVIPSLPSIYDEPFADASQVPTLLVSRLARKQVTVALSGDGGDELF
                     AGYNRYSLAARNWGRISKLPRPLRGALAKGLVALSPGQWNRVAGAVAPILPASLRLSL
                     PGEKVHKAARGLASASADDLYRGLVSSWRDPGEIVIGGPEPATQSAETLDRLGGLGTC
                     ERMMALDMLTYLPDDILAKVDRAAMSVSLETRVPLLDHRVVEFAWRLPLDLKLRGGES
                     KWVLRQLLYRHVPRALIERPKMGFGIPIDGWLRGPLKGWAEELLSERRLAGEGYFRPE
                     PIRAAWQAHQSGRANMQYQLWAILMFQSWLEGQAAPEEAEGLAA"
BASE COUNT          254 a          539 c          510 g          224 t
ORIGIN      
        1 gtgcgcgaga agctgcagga gctgatcgcc cccgaccagg gcgcgctcgg gaaacccgat
       61 ccggccgagc ggccggaggg gatctcgagg gatacgaggg tggtcagcta aatccgcgtc
      121 attccggcga aggccggaac ccatgaacac ggatcgccgc gatcatccac cttcgcccgt
      181 gttcatggat cccgggtcaa gcccgggatg acgatcacgc cgccagccct tccgcctcct
      241 cgggcgccgc ctggccttcc agccacgact ggaacatcaa tatcgcccag agctgatatt
      301 gcatgttcgc ccgcccgctc tggtgcgcct gccaggcggc ccggatcggc tcgggccgga
      361 aatagccttc gccggcaagc cgccgctcgg agagcaattc ctccgcccag cccttgagcg
      421 gcccgcgcag ccagccgtcg atcgggattc cgaaccccat cttgggccgc tcgatcagag
      481 cgcgcgggac gtggcggtag agcagctggc ggagcaccca tttcgattcg ccgccgcgaa
      541 gcttcaggtc gagcggcagc cgccaggcga attcgaccac cctgtggtcg agcagcggca
      601 ccctcgtctc gagcgagacg ctcatcgccg cccggtcgac cttggccagg atgtcgtccg
      661 gcagataggt cagcatgtcg agcgccatca tccgctcgca ggttccaagg ccgccgagcc
      721 ggtcgagggt ctcggcgctt tgggtggcgg gctccgggcc tccgatgacg atctcgcccg
      781 ggtcgcgcca gctcgacacc agcccccgat agagatcgtc ggccgaagcg ctggccaggc
      841 cccgcgccgc cttgtggacc ttctcgcccg ggagcgacag tcgaagcgag gcggggagga
      901 tcggagcgac ggcgcccgcg accctgttcc actgcccggg cgaaagcgcc accaggcctt
      961 tggcgagcgc cccgcgcagc ggccggggca gcttcgagat ccggccccag ttgcgggccg
     1021 ccagcgaata gcggttgtag ccggcgaaca attcgtcacc gccgtccccc gacagcgcca
     1081 ccgtgacctg tttgcgcgcc agccgggaca cgagcagggt cgggacctgc gaggcgtcgg
     1141 cgaaaggctc gtcgtagatg gacggcaggg aagggatgac cacgagcgcg tcctccgccc
     1201 gaacgtagag ctccgtgtgg tcggtgccga gatggcgggc gaccgctccg gcatgctcgg
     1261 cctcgttata ggccggatcc tcgaacccga tcgagaagct cttcaccggc cgcgacgatt
     1321 gcgcctgcat gagcgccacg atcgtcgagg aatcgacgcc tcccgacagg aaggctccga
     1381 gcggcacgtc ggacatcatc tgcttgccga ccgccgagaa gagcagcttc tcgagctcgt
     1441 cgaccccctc ttccgggctc gcatcgaccg ggttcgcgac gccggcttcc gccacttcgg
     1501 aagcggacca ataatggtgg atgacgg
//
LOCUS       DASWNU010000092         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_419151, whole genome shotgun sequence.
ACCESSION   DASWNU010000092 DASWNU010000000
VERSION     DASWNU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419151"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2771
                     /locus_tag="VGB57_01795"
     CDS             <1..2771
                     /locus_tag="VGB57_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0130107.1"
                     /translation="AIALIAGPALAQDAPTAAEQAAAQDEAAAEADAQPAPDSTIIVT
                     GTRAANRTAADSPVPIDVIGSEAIVNSGQTETNKILNQLVPSFNFPQPSIADGSDALR
                     PATLRGLSPDQTLVLINGKRRHVSSLLNINGTVGRGSAAVDMNLIPGLAIGRVEVLRD
                     GAAAQYGSDAIAGVINIQLKTNSNGGRASVTWGKYFTTVDNVANVTGLQLNGSGQPQL
                     DPSDSRYFLANSNGDRDADDGRQLTIGANIGIPMGPNGYFNITGEFRDRNRTNRAGFD
                     LRPNYVTGLPTFDSREIGFNRLQFRFGDPDTVDYNLFVNAGMEIAPGWELYGFGSYGH
                     RDTVSAANYRQAGAAANRDFSGLQPNTPLTPANFVALTPDGFLPLIDTDLDDLAGTIG
                     LRADLAGWKADLSFGHGQNRFDYLVRDTLNTSFGPLSQREFDAGGLFYAQDIANLDFS
                     RQFEMGFAKPLSVAAGTEYRHENFKIRPGDAQSSAAGPLFRAPIVNTTAANCTTQGGR
                     FTPATNLCDFPGRTAPAGAQGFPGIPASSATDVGRHSWAAYVELDTDPIEGVTTTIAG
                     RYERFSDFGSTWNGKFAARWEPVEGFALRGSISNGFRAPSLHQQFFTTTSTNFIGGLA
                     VDIATLAVGSPAARALGAQDLKPEKSLNMSLGATANPMRGLTITADVYQIDIDDRIVL
                     TETLGNGGTGNSGTVQQAVTNLLIASGFPQVSAARFFINGLDTRTKGLDVVAVYRRNL
                     QNLGNWSLSAAYNYNKTTIEERLPALGPLATIPGLVLFGRVEGLRFTHGQPRDKIVLS
                     ADGDIGNFGITARTTRYGKVVSPGAASPLAPNAASLTAFAPDDIFLKAKWITDLELRY
                     KAFDRLELAIGANNLFDVYPTRSPFGARPASIGGVYPANQEYIPFSIFSPFGFNGRFL
                     YGRVGIEF"
     gene            complement(2939..>3207)
                     /locus_tag="VGB57_01800"
     CDS             complement(2939..>3207)
                     /locus_tag="VGB57_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130108.1"
                     /translation="ALGPDDIELGAKWITDVELRYKAFDRLELAVGADNVFDVYPDRS
                     PIGPRPASQGGGVYPINQYYLPYSGFSPFGFNGRFVYGRIGIEF"
BASE COUNT          553 a         1237 c          935 g          482 t
ORIGIN      
        1 ccgcgatcgc actgatcgca ggtccggcgc ttgcccagga tgcgccgacc gccgcggagc
       61 aggccgccgc gcaggacgag gccgccgccg aggcggacgc ccagcccgcc ccggattcga
      121 cgatcatcgt caccggcacc cgcgccgcca accgcaccgc cgccgacagc ccggtgccga
      181 tcgacgtcat cggctctgaa gcgatcgtca attccggcca gaccgagacc aacaagatcc
      241 tcaaccagct ggtgccgagc ttcaacttcc cgcagccctc gatcgccgac ggctccgacg
      301 ccctgcgccc ggccacgctg cgcggcctca gcccggacca gaccctggtc ctgatcaacg
      361 gcaagcgccg ccacgtctcc tcgctgctca acatcaacgg gacggtcggc cgaggcagcg
      421 ccgccgtcga catgaacctc atcccgggcc tcgccatcgg ccgggtcgag gtgctccgcg
      481 acggcgccgc cgcccaatat ggctcggacg cgatcgccgg cgtgatcaac atccagctca
      541 agaccaacag caacggcggc cgcgcctcgg tcacctgggg caaatatttc acgaccgtcg
      601 acaatgtcgc caacgtcacc ggcctccagc tgaacggctc cggccagccg cagctcgacc
      661 cgtccgacag ccgctatttc ctcgccaatt cgaacggcga ccgcgacgcc gacgacggcc
      721 gccagctcac catcggcgcc aatatcggca ttccgatggg ccccaacggc tatttcaaca
      781 tcaccggcga gttccgcgac cgcaaccgca ccaaccgcgc cggcttcgac ctcaggccca
      841 attacgtcac ggggctgccc accttcgact cgcgcgagat cggcttcaac cgcctccagt
      901 tccgcttcgg cgaccccgac acggtcgact acaatctgtt cgtcaatgcc ggaatggaga
      961 tcgccccggg ctgggagctc tacggcttcg gctcctacgg tcaccgcgac acggtcagcg
     1021 ccgccaacta ccggcaggcg ggcgccgccg cgaaccgcga tttcagcgga ctgcagccga
     1081 atacgccgct gacccccgcg aacttcgtcg ccctgacccc cgacggcttc ctgccgctca
     1141 tcgacaccga cctcgacgat cttgccggca ccatcggcct gcgcgccgat ctggccggct
     1201 ggaaggccga cctctccttc ggccacggcc agaaccgctt cgactatctc gtgcgcgaca
     1261 cgctcaacac ctccttcggg ccgctgagcc agcgcgagtt cgacgcgggc gggctcttct
     1321 acgcccagga catcgccaac ctcgatttct cgaggcaatt cgagatgggc ttcgccaagc
     1381 cgctctcggt cgcggccggc accgaatatc ggcacgagaa tttcaagatc cggcccggcg
     1441 acgcccagtc ctccgcggcc ggccccttgt tccgggcgcc gatcgtcaac accaccgccg
     1501 ccaactgcac cacccagggg gggcggttca cccccgcgac gaacctgtgc gacttccccg
     1561 gccgaaccgc cccggcgggc gcccagggct tccccggaat ccccgcctcc agcgcgaccg
     1621 acgtcggccg ccacagctgg gcggcctatg tcgagctcga caccgatccg atcgaagggg
     1681 tgaccaccac catcgccggc cgctacgagc gtttctccga cttcggctcg acctggaacg
     1741 gcaagttcgc cgcgcgctgg gagccggtcg agggcttcgc cttgcgcggg tcgatctcga
     1801 acggcttccg cgcgccgtcg cttcaccagc aattcttcac caccacctcg accaacttca
     1861 tcggcggact cgccgtcgac atcgcgacgc tggcggtcgg ctcgcccgcc gccagggctc
     1921 tcggcgccca ggatctgaag ccggagaagt cgctcaacat gagcctcgga gcgaccgcca
     1981 atccgatgcg cggcctcacc atcaccgccg atgtctacca gatcgacatc gacgaccgga
     2041 tcgtccttac cgagactctg ggcaacggcg gcaccggcaa cagcggcacg gtgcagcagg
     2101 cggtcaccaa cctcctcatc gccagcggct tcccgcaggt ctccgcggcg cgcttcttca
     2161 tcaacggcct cgacaccagg accaaaggcc tcgacgtcgt cgcggtctat cggcgcaacc
     2221 tgcagaatct cggcaactgg tcgctgagcg ccgcctacaa ttacaacaag acgacgatcg
     2281 aggagcgcct tcccgcgctc gggccgctcg cgaccatccc cggcctcgtc ctgttcggcc
     2341 gggtcgaggg gctgcgcttc acccacggcc agccgcgcga caagatcgtg ctcagcgccg
     2401 acggcgacat cggcaatttc gggatcaccg cccgaaccac ccgctacggc aaggtcgtct
     2461 cgccaggcgc cgcgtcgccg ctcgctccca atgcggcgag cctgaccgcc ttcgccccgg
     2521 acgacatctt cctcaaggcc aagtggatca ccgacctcga gctgcgctac aaggccttcg
     2581 accggctcga gctggcgatc ggcgccaaca atctgttcga cgtctacccg acccgctcgc
     2641 ccttcggagc gcgcccggcg tcgatcggcg gagtctatcc ggccaaccag gaatatatcc
     2701 ccttctcgat cttctcgccc ttcggcttca acggccgctt cctgtacgga cgggtgggaa
     2761 tcgagttcta ggcgtatcga cgttcagcga gctcgggctt ctaaagtcct cccctgcatt
     2821 tgcaggggag ggggaccatg cgacgcatgg tggaggggcc actctgactt cgaaggaccc
     2881 ctccaccgcg cttcgcgcgg tccccctccc ccggaaatcc gggggaggat ttgcgacttt
     2941 agaactcgat cccgatccgg ccgtagacga agcggccgtt gaagccgaac ggcgagaagc
     3001 ccgaataggg cagataatat tggttgatcg gatagacgcc gccgccctgc gaggcggggc
     3061 ggggcccgat cggcgaacga tccggataga cgtcgaacac gttatccgcg ccgaccgcca
     3121 gctcgagccg gtcgaaggcc ttgtagcgca gctcgacgtc cgtgatccac ttggcgccga
     3181 gctcgatgtc gtccggcccc agtgccg
//
LOCUS       DASWNU010000093         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_314442, whole genome shotgun sequence.
ACCESSION   DASWNU010000093 DASWNU010000000
VERSION     DASWNU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314442"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGB57_01805"
     CDS             complement(<1..393)
                     /locus_tag="VGB57_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130109.1"
                     /translation="MDDGRPTLEVGFAIDTGGSYQELIQLQQVMDSAEAKIVADAASI
                     ERATETMVDTRAATASIRSFGGAATKEAASAARELAKIERAGEGLARQLERDAAFFGK
                     TRDEIRSMKVEAAALAAEQKGLTELAGRL"
     gene            475..918
                     /locus_tag="VGB57_01810"
     CDS             475..918
                     /locus_tag="VGB57_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130110.1"
                     /translation="MLAVLLFAAAQAPALEDRAQFEVFWRENARIAAAAAVSKEAAWI
                     LWEWTQAQFSLGFCSQFISADVAENMRHIPDPDGKLAGTKLGRWFLDQSRASFREGMA
                     FRAEVRPTLRLCEKELRERGETLEALKSIELVRPGPEGSAEHRPK"
     gene            complement(895..1260)
                     /locus_tag="VGB57_01815"
     CDS             complement(895..1260)
                     /locus_tag="VGB57_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130111.1"
                     /translation="MQGDQAARVSRLEQLKKDGIADPPMPPNPAPHIVAWLIEIGLTE
                     SNGMAAAPLSWRELSAWQDNTGVPLAAWESRLIRQLSVAYAAESRRAESENCPPPWRT
                     EVTQREIEAAEASLRAVLG"
     gene            complement(1341..1757)
                     /locus_tag="VGB57_01820"
     CDS             complement(1341..1757)
                     /locus_tag="VGB57_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130112.1"
                     /translation="MFDITTQAVNDTATIHIKGADGELLYADADRTLPCEIVIYGPGS
                     KAFGLLEARQSARVIKRMQENDGKITVAPFEERARETAEDLASITVAFNNFDYPPAGD
                     AQGVELFTALYSAPRLGFIPRQVTKHLGDWGNFRAA"
     gene            complement(1874..2323)
                     /locus_tag="VGB57_01825"
     CDS             complement(1874..2323)
                     /locus_tag="VGB57_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130113.1"
                     /translation="MGSTTAAGSTLAISAGNPATEDAAGYAALVYTEVGQIEKIGGLG
                     ATFAKVEFQPLKGAKQKHKGSVDYGSLAPTMAHDSTDAGQTLVRTASDDATSKLYSFL
                     VTYPTGEKRYCQGRVFGYPETVEGADSVITAAPTIEICTKIVKVAGA"
     gene            complement(2395..2670)
                     /locus_tag="VGB57_01830"
     CDS             complement(2395..2670)
                     /locus_tag="VGB57_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130114.1"
                     /translation="MTETRKRAVIVSSFNDAGTGKNYVAGSTRMIAAGAFANYEAAGL
                     VREPAPRQVRPKASTRSKAKAKPPAALPPTIPAEPAAPALGEDRPSA"
     gene            complement(2696..3091)
                     /locus_tag="VGB57_01835"
     CDS             complement(2696..3091)
                     /locus_tag="VGB57_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130115.1"
                     /translation="MTGSDIVGALVTSDQDLIAIVLEASIKFGALPEGVILPALLIQL
                     VSSIERQPLKRGATTRTVDRIAVTVRAASYRDQRAVIALLKKCCAGRTGDLGGAKSVS
                     IQTAGTGPDLRGPGNTFEQTQDFRVSYDA"
     gene            complement(3078..3626)
                     /locus_tag="VGB57_01840"
     CDS             complement(3078..3626)
                     /locus_tag="VGB57_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEY0130116.1"
                     /translation="MPTVRGKEAVKSFIGLIPAELEDKVFRGAARAAATVVATEAKAR
                     THSSEVRGAIKVSVRRPEAGQVLALVQVKGPGSYIAPWEEYGTAPHLISVDESQRGGM
                     SIGRINRLAKDPDSNHSLVIGGKFVGKTVLHPGARAHPFLRPALDIKEAAAVAAAQGY
                     INSRVTRAGITGGPEGGDDDGQ"
     gene            complement(3626..>3805)
                     /locus_tag="VGB57_01845"
     CDS             complement(3626..>3805)
                     /locus_tag="VGB57_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEY0130117.1"
                     /translation="TRPARIRIRFREDVTADMRFVLGSRVMQIVAGPAEATDLEPRQA
                     VDFMVEDYRPAGNPA"
BASE COUNT          534 a         1241 c         1355 g          675 t
ORIGIN      
        1 caggcgtccg gcgagctcgg tgaggccctt ctgctcggcc gccagcgcgg ccgcctcgac
       61 cttcatcgag cggatctcgt cgcgggtctt gccgaagaag gcggcatccc gctcgagctg
      121 gcgggccaag ccctcgccgg cgcgctcgat cttggccagc tcgcgcgcgg cgctcgccgc
      181 ctccttggtc gcggcgccgc cgaagctgcg aatgctggcc gtcgcggcgc gggtgtcgac
      241 catggtttcg gtcgcgcgct caatgctggc cgcgtcggcg acgatcttcg cctcggccga
      301 gtccatgacc tgctgaagct ggatcagctc ctgataggag ccgccggtgt cgatcgcgaa
      361 gccgacctcg agagtgggcc gaccgtcatc cactgctgcg gactccaaac gcggccgcaa
      421 tgaagctatc gctcatcggt cacctcgact cgggaagaag gaagagaatc ggcgatgctg
      481 gcggttttgc tattcgcggc cgcccaggcc ccggccctcg aggaccgcgc gcaattcgag
      541 gtcttctggc gagagaacgc cagaatcgcc gcggccgcgg ccgtctcgaa ggaggcggcc
      601 tggatcctat gggaatggac ccaggctcaa ttttcgctcg gtttctgctc tcaattcatc
      661 agcgccgacg tcgccgagaa catgcggcac attccggatc cggacggaaa gctggcgggt
      721 acgaagctcg gccgctggtt tctggaccag agccgagctt cgttccgcga gggcatggcg
      781 ttccgggccg aggtccggcc caccctgcgc ctttgtgaga aggagctccg cgagcgcggg
      841 gagacgctcg aggcgctcaa gtcgatcgag ctggtgaggc cgggcccgga aggctcagcc
      901 gagcaccgcc cgaagtgacg cctcggccgc ctcgatctcg cgttgcgtga cctcggtccg
      961 ccagggcggg gggcagttct cgctctccgc acgtcgactt tcggccgcat aggcaacgga
     1021 gagctgccgg atcaggcgcg actcccaggc ggccagaggg acgcctgtgt tgtcctgcca
     1081 tgcgctcagc tcgcgccagc tgagcggtgc cgccgccatg ccgttcgatt cggtgagccc
     1141 gatctcgatc agccacgcga cgatatgcgg cgccgggttg ggaggcatcg gcggatccgc
     1201 gatgccgtcc tttttgagtt gctccaggcg gctgacgcgc gccgcctggt ccccttgcat
     1261 agcccgcttc gtcccttcgg ccggcttcgg cgtggcatga agccacgcca tctgccggac
     1321 gaagagcttc agctcacggg ctatgcggcc ctgaagtttc cccagtcgcc gaggtgcttc
     1381 gtgacctggc gggggatgaa gccgagcctc ggagcggagt agagggcggt gaagagctcg
     1441 acgccctggg cgtcaccggc cggcggatag tcgaaattgt tgaaggcgac ggtgatcgag
     1501 gccaggtcct cggccgtctc ccgggcccgc tcctcgaacg gggcgaccgt gatcttgccg
     1561 tcgttctcct gcatccgctt gatgacgcgc gccgactggc gggcctcgag caggccgaag
     1621 gccttgctgc cggggccata gatgacgatt tcgcagggca aggtgcgatc ggcgtcggcg
     1681 tagagcagct cgccgtccgc gcccttgatg tggatcgtgg cggtgtcgtt gacggcctgg
     1741 gtggtgatat cgaacatggt atttcctcgc ggttgagatg caccgacccg ctcccgcccg
     1801 cgagtgcgcg gaagcgggcc gatgcctgtg gaccggctca gggccgggat tgtgttgggt
     1861 taggggcggc gccttaggcg ccggcgacct tcacgatctt cgtgcagatc tcgatcgtcg
     1921 gggccgcggt gatgacgctg tcggcgccct cgacggtctc ggggtagccg aagacgcggc
     1981 cctggcagta gcgcttctcg ccggtcggat aggtgaccag gaacgagtag agcttcgacg
     2041 tcgcatcgtc cgaggccgtg cggaccaggg tctggccggc gtcggtcgaa tcgtgggcca
     2101 tggtcggggc gagagagccg tagtcgaccg agcccttatg cttctgcttc gcgcccttca
     2161 ggggctggaa ctcgaccttc gcgaaggtcg cgccgagacc gccgatcttc tcgatctggc
     2221 cgacctcggt gtagacgagc gcggcgtagc cggccgcgtc ttcggttgcc ggattgccgg
     2281 cggaaatggc gagcgtcgag cccgccgcgg tggttgaacc catctaaaat ctcctcgagg
     2341 gttgtggcga gccggcgagg ccggcggtct tcagcccggg gatcgggcgg aatctcaggc
     2401 gctggggcgg tcctcgccca aggcgggcgc ggcgggctca gcgggaatgg tgggcggcag
     2461 cgccgccggc ggcttggcct tggccttcga cctggtggac gccttcggcc ggacctgcct
     2521 cggggccggc tcgcggacca ggccggccgc ttcgtaattc gcgaaggcgc cggcggcgat
     2581 catgcgggtg gagccggcga catagttctt gccggtgccg gcgtcgttga acgagctgac
     2641 gatcaccgcc cgcttcctgg tttcggtcat gttgatttgc ctcgctgctg gggcgtcagg
     2701 cgtcgtagct gacgcgaaaa tcttgggtct gttcgaaggt gttgccgggg ccccggaggt
     2761 ccggcccggt gccggcggtt tggatcgaga ccgacttcgc tccccccagg tcgccagtgc
     2821 ggccggcgca gcacttcttc aggagcgcga tcaccgctcg ctggtcgcgg tagctggcgg
     2881 ctctgacagt gaccgcgatc cggtcgacgg tcctggtggt cgcgcctcgc ttcagcggct
     2941 ggcgctcgat cgagctcact agctggatca gcagcgccgg gaggatgacg ccctcgggga
     3001 gagcgccgaa cttgatgctg gcctcgagga cgatcgcaat gaggtcctgg tcgctggtca
     3061 ccagggcgcc gacgatgtca ctgcccgtca tcgtcgcccc cttccggacc gccggtgatc
     3121 ccggctcgag tcacccgaga attgatgtag ccctgggccg cggcgaccgc ggcggcctcc
     3181 ttgatatcca gcgccgggcg caggaacggg tgagctcgcg cgccggggtg aaggaccgtc
     3241 ttgccgacga acttgccccc gatgaccagg gaatgattgc tgtcgggatc cttggcgagg
     3301 cggttgattc gcccgatgct catcccgcca cgctgactct cgtcgacgct gataaggtgc
     3361 ggcgcggtgc cgtattcctc ccaaggcgcg atataggacc ctggcccctt cacctggacc
     3421 agtgcgagga cctggccggc ttccggccgg cggaccgaga ccttgatcgc gccgcggact
     3481 tcgctcgagt gggttcgggc cttggcctcg gtggcgacca ccgtggcggc cgccctagcg
     3541 gcgccgcgaa agaccttgtc ctcgagctcg gccgggatca ggccgatgaa gctcttcacg
     3601 gcctccttgc cgcgcacggt tggcatcagg ccgggttccc ggccgggcga taatcctcga
     3661 ccatgaagtc gacggcctgg cgcggctcga ggtcggtcgc ctcagctggg ccggccacga
     3721 tttgcatgac gcgggatccg agaacgaacc gcatatcagc ggtcacgtcc tcgcggaatc
     3781 gtatgcggat ccgcgccggc ctggt
//
LOCUS       DASWNU010000094         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_496824, whole genome shotgun sequence.
ACCESSION   DASWNU010000094 DASWNU010000000
VERSION     DASWNU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496824"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..892)
                     /locus_tag="VGB57_01850"
     CDS             complement(245..892)
                     /locus_tag="VGB57_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0130118.1"
                     /translation="MIVYGASLSPFVRKVLAYGAEKGLELEHKPLGLGSTDPDFLAAS
                     PFRKIPAFSDGDFGISDSSAIIHYLEAKYPDPALIPAEPKARARVIWFDEFADTILCA
                     CGAKMFFNRIVAPRFLGREGDLETADKAEKEELPPILDYLERVIPESGWLVGEGITLA
                     DIAVATAFANFRHLGMTIDAGRYPKVAAFADRMLARPSFAGWVERETAFLEKTAA"
     gene            complement(943..2961)
                     /locus_tag="VGB57_01855"
     CDS             complement(943..2961)
                     /locus_tag="VGB57_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0130119.1"
                     /translation="MPFLLSLLLAQATPDIVVTGRGLAPAAGEEVFDTIVIDRDRLTG
                     SASGRMEEVLRDIPGFQLFRRSDSRSANPTSQGATLRALGGNASSRVLLVLDGVPQTD
                     PFGGWVSWPAYHPERLGEARVTRGGGSGVQGPGALAGTIELASATPADLRGPQGRVAY
                     GSRDGFDAFAGLGLPLGGGFVALSGAWARGDGFIPIVEAQRGPADRPSPYEQTSLAVR
                     GVAPLGGAELQASGLVFSDARERGTAFSAIRTRGADASLRLVGRRWSALAYVQTRDFY
                     NSFASVNAGRTAATRTSEQFSVPATGLGARVEARPNVGSNVELRLGGDWRLTDGETRE
                     LFAFVAGAGTRGRVAGGRSQTYGGFAETAWTADALTLTAGARIDRWTISNGRLHERVL
                     ATGAVLTDTAFADRSGWKPTGRAGIAWRPDEGLSLRGAAYLGWRLPTLNELYRPFRVG
                     IDATAANAGLAPERLRGVEAGIDWRPAPRARIGLTVFSNRLEGAIANVTLGRGPGNFP
                     GVGFVAAGGDYRRRGNLDSIAAEGIEVEGRYALGDWSLSGGWSRVHSEVEASGSALAL
                     DGLRPAQTPRDSLSSTLAWASGRTRASVTARYTGAQYEDDLNRQRLPGAFTLDAVGRL
                     PLGRRLAVEARAENVANARILAGISGDGIQERATPRTFWIGLRFGRLQ"
     gene            complement(3111..>3609)
                     /locus_tag="VGB57_01860"
     CDS             complement(3111..>3609)
                     /locus_tag="VGB57_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0130120.1"
                     /translation="SNAIHGNKSQPQRERALAEFKSGKVKVLIATDIAARGIDVSGVS
                     HVINFELPNVAEQYVHRIGRTARAGAAGVAIAYCAEDERPYLKGIERLTKQTLTPVPL
                     PEGFNAEAARIKTARANSPIQIVERPEREERHSRGRPAKRFSARPARPQGESRSGGRR
                     RAASR"
BASE COUNT          532 a         1259 c         1281 g          537 t
ORIGIN      
        1 gctcggcctt caggttcttc gcgccctgca gcagctgctc gatctcgtcg cgctggccgc
       61 cctgcagagt cgcgcgcacg gcctggtcgt agggagggtg aaccgcccga gtcctcgacg
      121 tgggtgcttt gcttgtcggt gttttcacca tggtccgtct cccggctccg gttgagtcga
      181 agccgggata ataaaacgga cgatggcgag ggcacggaga agttggtcga aaacggacct
      241 ttcgtcaggc cgccgtcttc tcgaggaagg cggtttcccg ctcgacccag cccgcaaagc
      301 tcggccgtgc cagcatccgg tcggcgaagg cggccacttt cggatagcgc ccggcgtcga
      361 tcgtcattcc aagatggcgg aaattggcga aggccgtcgc gaccgcgatg tcggcgaggg
      421 tgatcccttc ccccaccagc cagccgctct cgggtatgac acgctcgaga tagtcgagga
      481 tggggggaag ctcctccttt tcggccttgt cggcggtctc gaggtcgcct tcccggccga
      541 ggaaacgcgg agcgacgatg cggttgaaga acatcttggc cccgcaggcg cagaggatgg
      601 tgtcggcgaa ctcgtcgaac cagatcaccc gcgcccgcgc cttcggctcg gccgggatca
      661 gcgccgggtc aggatatttc gcttcgagat agtggatgat cgcggatgaa tcggagatcc
      721 cgaaatcgcc gtcgctgaag gccggtatct tccggaacgg gcttgccgcg aggaagtccg
      781 gatcggtcga gccgaggccg agcggcttgt gctccagctc cagccccttc tccgcgccat
      841 aagccagcac cttgcgaacg aaaggcgaca gcgaagctcc gtagacgatc atgtccgatc
      901 tcccttttaa gtttcaagac gcaacctata cggctgcgcc cttcactgca agcggccgaa
      961 tcgaagcccg atccaaaagg tgcgcggagt cgccctttcc tggattccgt cgccgctgat
     1021 cccggcgagg atccgcgcat tggcgacatt ttcggcgcga gcctcgaccg ccagcctgcg
     1081 cccgagcggg agccggccga cggcgtcgag agtgaacgcg ccgggcaggc gctggcggtt
     1141 gagatcgtcc tcatactggg cgccggtgta gcgcgcggtg acggaggcgc gggtgcggcc
     1201 cgaagcccag gccagggtgg acgacaggct gtcgcgcggg gtctgggccg gcctcaggcc
     1261 gtcgagcgcc agcgcggacc cgctcgcctc gacctcggaa tggacccggg accagcctcc
     1321 cgagaggctc cagtcgccga gcgcgtagcg gccctccacc tcgatccctt cggccgctat
     1381 ggaatcgaga ttgccccgcc gccgatagtc gccgccggcg gcgacgaagc cgacgcccgg
     1441 gaaattgccc gggccccgcc ccagggtgac gttggcgatc gcgccttcca ggcggttgga
     1501 gaagacggtg aggccgatcc gggcccgggg cgccggccgc cagtcgattc ccgcctcgac
     1561 cccgcgaagc cgctcgggcg ctaacccggc attggccgcg gtcgcgtcga tcccgacccg
     1621 gaacggccgg tagagctcgt tcagggtcgg aagtcgccag ccgagatagg ctgcgccgcg
     1681 caggctcagc ccctcgtccg ggcgccaggc gatcccggcc cgccccgtcg gcttccagcc
     1741 cgagcggtcg gcgaaggccg tgtcggtcag gaccgcgccg gtcgcgagca cccgctcgtg
     1801 aaggcggccg ttcgagatgg tccagcggtc gatccgggcg ccggcggtca aagtcaaagc
     1861 gtccgccgtc caggcggtct cggcgaagcc gccataggtc tggcttcggc cgcccgccac
     1921 ccgcccgcgg gtgccggcgc cggcgacgaa ggcgaaaagc tcgcgggtct cgccgtcggt
     1981 cagccgccag tcgccgccga gcctgagctc gacgttcgag ccgacgttcg gcctcgcctc
     2041 aacccgggcg ccgaggccgg tcgccgggac gctgaactgc tcggaggtgc gggtcgccgc
     2101 ggtccgcccc gcattgacgc tggcgaagct gttgtagaag tcccgggtct ggacgtaggc
     2161 gagagcgctc caccgccgcc cgaccagacg gagcgacgcg tcggctccgc gcgtccggat
     2221 cgcggagaaa gccgttcccc gctcgcgggc gtcggaaaaa accagcccgc tcgcctgaag
     2281 ctccgcgccg ccaaggggcg ccacgccgcg caccgcgagg ctcgtctgct cgtaaggcga
     2341 gggccggtcc gcggggccgc gctgcgcctc gacgatcggg atgaacccgt cgccccgcgc
     2401 ccaggcgccc gagagtgcga cgaagccgcc gcccagcggc aggccgagcc ccgcgaaggc
     2461 gtcgaagccg tcgcggctgc cgtaggcgac tcgcccctgc ggcccgcgca ggtccgccgg
     2521 ggtggcgctg gccagctcga tcgtgccggc aagcgctccc ggcccctgaa cgccgctgcc
     2581 gccgccgcgg gttacccgcg cctcgccgag ccgctcggga tggtaggccg gccagctcac
     2641 ccagccgccg aacgggtcgg tctgcggaac cccgtcgagg acgagcagga cccggctcga
     2701 cgcattgccg ccgagcgccc tgagcgtcgc gccctggctg gtcgggttgg cggagcggga
     2761 gtcggaccgg cggaagagct ggaagccggg aatgtcgcgc agcacctcct ccatccggcc
     2821 ggaggcggag ccggtgaggc ggtcgcggtc gatgacgatc gtgtcgaaca cctcttcgcc
     2881 ggcggcgggg gccaggcctc ggccggtgac gacgatgtcg ggagtcgcct gggcgagcag
     2941 cagcgagagg agaaacggca cggtcggcct ctaaattcct cccccggatt tccgggggag
     3001 ggggaccgcg cgaagcgcgg tggaggggtt cttcggagtc agcaaaggcc cctccaccac
     3061 ccttcgggtg gtccccctcc cctgcgaaag caggggagga tttttacgac ttacctggaa
     3121 gcggccctac gacgcccgcc gctgcggctc tcgccctgcg ggcgggccgg gcgggcgctg
     3181 aagcgcttgg cggggcgtcc gcgggaatgc cgctcctcgc gctccgggcg ctcgacgatc
     3241 tggatcggcg agttggcgcg ggccgtcttg atccgggccg cttcggcgtt gaagccttcc
     3301 ggcagcggca ccggcgtgag cgtctgcttg gtcaggcgct cgataccctt caggtaaggc
     3361 cgctcgtcct cggcgcaata agcgatcgca acgcccgccg cgcccgctcg agcggtgcgg
     3421 ccaatccggt gcacatattg ctcggcgacg ttcggaagct cgaagttgat gacgtgactg
     3481 acgccggaca cgtcgatccc gcgcgcggcg atgtccgtcg caatgagcac cttcaccttg
     3541 ccgctcttga actcggcaag cgcccgctcg cgctgcggct ggctcttgtt gccgtggatg
     3601 gcattggac
//
LOCUS       DASWNU010000095         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_4078,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000095 DASWNU010000000
VERSION     DASWNU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4078"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1840
                     /locus_tag="VGB57_01865"
     CDS             221..1840
                     /locus_tag="VGB57_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY0130121.1"
                     /translation="MDDDSSIFIGLGGGKPQRLMLKRANRHGLIAGATGTGKTVTLQG
                     LAEGFSSAGVPVFVADVKGDLGGLAMAGSPMGKLHDAFAARAAEIGDSGWAYGDNPVQ
                     FWDLLGRQGHPIRTTVSEMGPLMLARLMDLNEVQEGVLAIVFHVADKDGLLLLDLDDL
                     QAMLADCANRADELTVTYGNVSRASVGTIQRSLLQLRSQGGDRFFGEPALDIDDLIAL
                     DDRGRGIVNILAADRLMASPKLYSTFLLWLLSELFEQLPEVGDPDKPKLVFFFDEAHL
                     LFASAPAALLEKIEQVVRLIRSKGVGIYFITQNPIDIPDAVAGQLGNRIQHALRAFTP
                     RDEKAVRSAAETFRANPGVDVATAITELKVGEALVSLLQSDGSPSPVERTLIRVPGSR
                     VGPVTDEERAVLVQTDSIGTKYDEAIDRESAQELLGAKSSEAQAAAAEAKAKAEEAKL
                     DAAEARQAEKERLLAEREAARSAKEAERARVAAEREAARRAREAAKPTFTDKLVQSAA
                     RSAASSVGRQVAGKLGGQLLRGLLGGLFRGR"
BASE COUNT          292 a          655 c          624 g          270 t
ORIGIN      
        1 ttacggcgtc gcgctggcgg ggcggtaagc tgtctagatc ctcccttgca ttcgcagggg
       61 agggggacca tgcggagcat ggtggagggg cctttctgac cccaaagaac ccctccaccg
      121 cgctttgcgc ggtccccctc cccgacgaat gtcgaggagg aattttgtgt ccagctccac
      181 atcggatacg gcggccgacc ctattccggg agcgaaaagc atggacgacg acagttcgat
      241 cttcatcggc ctcggcggcg gcaagccgca gcggctcatg ctcaagcgcg ccaaccgcca
      301 cggcctgatc gccggggcga ccggcaccgg caagaccgtc accctccagg gcctcgccga
      361 aggcttctcg agcgccggtg tcccggtctt cgtcgccgac gtgaaaggcg atctcggcgg
      421 cctcgccatg gcgggatcgc cgatgggcaa gcttcacgac gccttcgccg cgcgggccgc
      481 cgagatcggc gattccggct gggcctatgg cgacaatccg gtccagttct gggacctgct
      541 cggccggcag ggccacccga tccggaccac ggtttcggag atgggcccgc tgatgctggc
      601 ccgcctgatg gacctcaacg aggtgcagga gggcgtgctc gcaatcgtct tccacgtcgc
      661 cgacaaggac gggcttctcc tgctcgacct cgacgacctc caggccatgc tcgccgactg
      721 cgccaaccgc gccgacgagc tgaccgtgac ctacggcaac gtctccaggg cgagcgtcgg
      781 caccatccag cgctcgctcc tccagctccg ctcgcagggc ggcgaccgct tcttcggcga
      841 gccggcgctc gacatcgacg acctcatcgc cctcgacgac cgcgggcggg gcatcgtcaa
      901 catcctcgcg gccgaccggc tgatggcctc gcccaagctt tattcgacct tcctgctctg
      961 gctcctctca gagctgttcg agcagcttcc cgaggtcggc gatccggaca agccgaagct
     1021 ggtcttcttc ttcgacgagg cgcacctgct cttcgccagc gccccggcgg ccctgctgga
     1081 gaagatcgag caggtcgtcc ggctgatccg ctcgaagggc gtcggaattt acttcatcac
     1141 ccagaacccg atcgacattc cggacgccgt cgccggtcag ctcggcaacc ggatccagca
     1201 cgcacttcgc gccttcaccc cgcgcgacga gaaagcggtg cgctcggcgg cggagacctt
     1261 ccgggccaat cccggagtag acgtcgcgac ggcgatcacc gagctcaagg tcggcgaggc
     1321 gctggtgtcc ctgctccagt ccgacggatc gccctcgccg gtcgagcgga ccctgatccg
     1381 ggtgccgggc tcgcgggtcg gcccggtcac ggacgaggaa cgggcggtgc tcgtccagac
     1441 cgactcgatc ggcaccaaat atgacgaggc gatcgaccgc gaatccgcgc aggagcttct
     1501 cggcgccaag tcgagcgagg cccaggccgc ggccgccgag gcgaaggcga aggcggagga
     1561 ggcgaagctc gacgccgccg aggccaggca ggccgagaag gagcggctcc tcgccgagcg
     1621 cgaggccgct cgctcggcca aagaggcgga gcgagcccgg gtcgccgccg aacgcgaggc
     1681 cgcccgcagg gcccgcgagg cggcgaaacc gaccttcacc gacaagctgg tccagtcggc
     1741 cgcccgctcg gccgccagct ccgtcggccg tcaggtcgcg ggcaagctcg gcggccagtt
     1801 gttgaggggg ctgctggggg ggctgttccg ggggcggtag c
//
LOCUS       DASWNU010000096         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_4203,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000096 DASWNU010000000
VERSION     DASWNU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4203"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGB57_01870"
     CDS             <1..452
                     /locus_tag="VGB57_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130122.1"
                     /translation="RAMGRSRPSPPAEAIRRLLTSVLKQSMPRAAAARSRAIIAPIFG
                     DRLAAIPGHSLGLQLEGLSEEDRRFETARQLVRFIAGAAKRGLAPSQLEPENTARAAA
                     FAAARQHAPGMTAGRRPGSSPRSGPGFEDRAGSRPFRLRPVAGPAGA"
     gene            519..1319
                     /locus_tag="VGB57_01875"
     CDS             519..1319
                     /locus_tag="VGB57_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130123.1"
                     /translation="MHNIDRNILESSFEAGPFETGEFAFEQYEAPLGHEFAETGQHEG
                     GFSNEYAQEGEAWAGEEETFEVFQGEGIFNEAQEVELAAELLEITNEGELDQFLGKLV
                     RKVGQFAKSPMGKALGGVLKGLAKKALPLAGTALGGMVGGPLGAKIGQGLASAAGSAI
                     GLEGEFSQEDREFAGARQFVRIADQTARQMAMGTRGDPRTAAQRAMMRSVRMSAPGLL
                     QGQSFGGYGPPAHAAGGYGASDGYGRSGGRRQSGSWMRRGNRIVLTGV"
     gene            1338..>2245
                     /locus_tag="VGB57_01880"
     CDS             1338..>2245
                     /locus_tag="VGB57_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130124.1"
                     /translation="MSPGAYARTMLEQEARSMLARLALVKPFALQEAVLPAAALLPVA
                     QSAIDKCLLIGRRELRNLLKGYVRWLRRIDDRITPAQAQRRFSILRMKFNVVLTQFDL
                     FADVITQRSESETGTWLGGLDVAATDALALPGYYDPPPVICYLDRDIGAAIRRARTRL
                     PGGVENPVAIIRVPRERMVGSGIASSLVHEVGHQAAALLDLVNSLRPVLQGLQRGASA
                     DRIAWSLWERWISEIVADLWSVARVGIGSTMGLVNVVSLPRAFVFRLNVDDPHPMPWL
                     RVKLSASIGEALYPHPQWRRIAALWES"
BASE COUNT          359 a          732 c          809 g          345 t
ORIGIN      
        1 gtcgggccat ggggcggagc cggccctcgc ccccggcgga ggcgatccgg cggctgctga
       61 catcggtgct caagcaatcg atgccccgcg ctgccgcggc caggagccgc gcgatcatcg
      121 cgccgatctt cggcgatagg ctggcggcca taccgggcca cagcctcggc ctccagctcg
      181 aggggctcag cgaggaggat cggcggttcg agaccgcccg ccagctggtc cgcttcatcg
      241 cgggcgctgc aaagcggggg ctcgccccat cgcaactgga gccggagaat acggcgcggg
      301 ccgcggcctt cgccgcggcg cgacagcacg cgcccggcat gacggcaggc cggcggccgg
      361 gatcgtcgcc tcgctccgga ccaggttttg aggatcgtgc cgggtcgagg ccattccggc
      421 tccgacccgt cgcagggccc gccggcgcct gaacaggtcc ctgttcgggc tccgacgatt
      481 tggtgagctc cgcccggagc cgcactcagg aggaagtgat gcacaatatc gatcgcaata
      541 tattggaaag cagtttcgag gccgggccgt tcgagaccgg cgagttcgcc ttcgagcaat
      601 atgaggcgcc gctcggccac gaattcgccg agaccggaca gcatgaagga ggcttttcca
      661 acgaatatgc tcaggagggt gaggcctggg ccggagagga agagactttc gaggtcttcc
      721 agggcgaagg catattcaac gaagcgcagg aggtggagct cgcggccgag ctgctcgaga
      781 taaccaacga gggcgagctc gatcaatttc tcggcaaact cgtccggaag gtcggtcagt
      841 tcgcgaaatc gcccatgggc aaggccctcg gcggcgtcct gaaaggcctc gcgaagaagg
      901 cgctgccatt ggccggaacc gcgcttggcg gaatggtcgg agggccgctc ggcgccaaga
      961 tcggccaggg cctggcatcg gcggcgggaa gcgcgatcgg cctcgagggc gagttcagcc
     1021 aagaggatcg cgaattcgcg ggcgccaggc agttcgtccg gatcgccgac cagacggcgc
     1081 ggcagatggc catgggaacg cgcggcgatc cgcgcaccgc cgcccagcgc gcgatgatgc
     1141 ggtccgtccg gatgagcgcg ccggggctgc ttcagggcca gtcgttcggc ggctacggac
     1201 cgccggccca cgcggctggc ggctatggcg cctccgacgg ctacggccgc tctggcggcc
     1261 gtcggcaaag cgggagctgg atgcgccgcg gcaatcgtat cgtcctgacc ggcgtctgag
     1321 ggaaggggga ctctgtcatg tcaccgggag cctatgcgcg aaccatgctc gagcaggagg
     1381 cgcgctcgat gctggcgcgc ctcgcgctgg tcaagccgtt cgcgcttcag gaagcagtgc
     1441 tgcctgcggc agcccttctc ccggtggcgc agagcgcgat cgacaaatgc ctgctcatcg
     1501 gccgccgcga actgcgcaat cttctcaagg gatatgtgcg ttggctgcgc aggatcgacg
     1561 accggatcac tccggcgcaa gcccagcgcc gcttttcgat cctgcggatg aagttcaacg
     1621 tcgtgctcac ccagttcgac ttgttcgcgg acgtaatcac ccagcgaagc gaaagcgaaa
     1681 cggggacgtg gctgggcggg ctcgacgtcg cggcgaccga cgcgctggcg cttcccggct
     1741 attacgaccc gccgccggtg atctgctatc tggaccggga cataggcgcg gcgatccggc
     1801 gggcccgcac ccggctgccc ggcggggtcg aaaatccggt cgcgatcatc cgggtcccgc
     1861 gggaacggat ggtcggcagc gggatcgcct cgtcgctggt ccacgaggtc ggccaccagg
     1921 cggcggcgct gctcgacctc gtcaattcgc tgcgcccggt cctgcagggg ctgcagcgcg
     1981 gcgcgagtgc ggaccggatc gcctggtcgc tgtgggaacg ttggatctcc gagatcgtcg
     2041 ccgatctgtg gtcggttgcc cgggtgggga tcggctcgac gatggggctg gtcaacgtcg
     2101 tcagcctgcc gcgggcgttc gtgttccggc tcaacgtcga cgatccgcat ccgatgccct
     2161 ggctccgggt gaaactgagc gcctcgatcg gcgaggccct ctacccccat ccgcaatggc
     2221 gcaggatcgc ggccctctgg gaatc
//
LOCUS       DASWNU010000097         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_471629, whole genome shotgun sequence.
ACCESSION   DASWNU010000097 DASWNU010000000
VERSION     DASWNU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471629"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VGB57_01885"
     CDS             complement(<1..1093)
                     /locus_tag="VGB57_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY0130125.1"
                     /translation="MASRSSFNPAIGFILVVAALDVMAIGIIIPVLPALVVEFTGSNA
                     HAGLWNGVMVALWAGMQFLFSPVIGSLSDRFGRRPVLLVSALGLAADFALMALAPNLW
                     WLALGRILGGITSSSFTTTFAYMADITEPAKRSRAYGLIGAAYSAGFVAGPVLGGLLG
                     EFGSRVPFWAAAALAGLSFVYGLLVIPESLKPELRTPFSWKRANPLGSLRLLRSHPGL
                     SGLAWVYFLIYVAHNVFAAVFVLYAGYRFGWSLWAVGLSLALWGVLDVIVQALLVGPA
                     VKRIGDRLTMIWGLVGGAAGLVVLGLAPDTLWFIIGIFVSALWGFSMPTIQALMTQRV
                     SESEQGQLQGANQSVASIASVLSPIFFGWI"
     gene            complement(1202..>2144)
                     /locus_tag="VGB57_01890"
     CDS             complement(1202..>2144)
                     /locus_tag="VGB57_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0130126.1"
                     /translation="GLIAFEDEGERVEQSILSGAAKLQWKVDWAMRWVALGVDYEMSG
                     KDLIDSVTQSSRIARILGARPPEGFNYELFLDENGEKISKSRGNGVTMDQWLTYAPQE
                     SLTFYLYREPRRAKQLSFGMIPKAVDEYNQFLAAYPNQPIEQKLGNPVHHIHGGEVPR
                     ARMPLTFALLLNLVGVASTDDKDLLWAFVRRYAPDVAPETHPEVDALIDHALAYFRDF
                     IAGSLARRPPSPLEAAALRDLDARLAALPRGADAETIQNQVYEVGKTHPFDNLRDWFR
                     ALYEILLGTSQGPRMGSFIALYGIANSRRLIAEALAA"
BASE COUNT          403 a          696 c          753 g          292 t
ORIGIN      
        1 agatccagcc gaagaagatc ggcgacagga cgctggcgat gctggcgacg ctctgattgg
       61 cgccctgcag ctggccctgc tcgctctcgc tcacccgctg cgtcatcagc gcctggatgg
      121 tcggcatcga aaagccccac agggcgctga cgaagatgcc gatgatgaac cacaacgtgt
      181 cgggcgcgag cccaagcacg acgaggccgg cggcgccgcc caccagcccc cagatcatcg
      241 tcagccggtc gccgatccgc tttacggcgg ggccgaccag cagcgcctgg acgatgacgt
      301 cgagcacgcc ccacagggcc agcgacaggc cgactgccca gaggctccag ccgaaccggt
      361 agccggcgta gaggacgaac acggcggcga agacgttgtg ggcgacgtag atcaggaaat
      421 agacccaggc gaggcccgac agccccgggt gcgagcgcag caggcgcagc gagccgagcg
      481 ggttggcgcg cttccaggag aagggcgtgc gcagctccgg cttgagcgat tccgggatca
      541 ccagcaggcc gtagacgaag gagaggccgg cgagcgcggc ggccgcccag aagggcaccc
      601 gcgagccgaa ttcgccgagg aggccgccga gcaccggccc cgcgacgaag ccggcgctat
      661 aggccgcgcc gatcagccca taagcgcgcg agcgcttcgc cggctcggta atgtcggcca
      721 tataggcgaa ggtggtggtg aagctggacg aggtgatgcc gcccaggatc cggccgagcg
      781 ccagccacca cagattgggc gccagcgcca tcagcgcgaa atccgcggcg aggccgagcg
      841 ccgagaccag cagcaccggg cggcggccga agcggtccga cagcgaccca atcaccggcg
      901 agaacaggaa ctgcatcccc gcccagagcg cgaccatgac gccgttccac aggccggcat
      961 gggcgttcga gccggtgaac tccaccacca aggccggcag caccgggatg atgatcccga
     1021 tcgccatcac gtcgagcgcc gcgacgacca ggatgaaccc gatggcgggg ttgaaagatg
     1081 accgggaggc caagggcaac gagcctatgt cccgaccgtc attgcaagga gcgcagcgac
     1141 gaagcaatcc agcggcgccg aaggtggatt gcttcgctgc gctcgcaatg acggcggagg
     1201 gtcatgcggc gagcgcctcg gcgatcaggc ggcggctgtt ggcgataccg tagagggcga
     1261 tgaagctgcc catgcggggc ccttgggaag tgccgagcag gatctcgtac agtgccctga
     1321 accagtcgcg cagattgtcg aacgggtgag tcttgccgac ctcgtagacc tggttctgga
     1381 tggtctcggc gtccgccccc cggggcaggg cggcgaggcg cgcgtcgagg tcgcgcaaag
     1441 cggccgcctc gaggggggac ggcgggcgcc tggccagcga gccggcgatg aaatcgcgga
     1501 aataagcgag cgcgtggtcg atcagcgcat cgacctccgg atgcgtctcc ggcgcgacgt
     1561 ccggcgcata tcggcgcacg aaggcccaga gcaggtcctt gtcgtcggtc gaggcgaccc
     1621 cgaccaggtt cagaagcaag gcgaaggtga gcggcattcg ggcccgcggc acctctccgc
     1681 catggatgtg atggaccggg ttgccgagct tctgctcgat cggctggttc ggataggccg
     1741 cgaggaattg attatattcg tccaccgcct tgggaatcat tccgaagctc agctgcttgg
     1801 cccgccgcgg ctcgcgatag aggtagaagg tcaggctttc ctggggcgcg taggtcagcc
     1861 actggtccat ggtcacgccg ttgccgcgcg acttggagat cttctcgcca ttctcgtcga
     1921 ggaacagctc gtagttgaat ccttccggcg ggcgggcgcc gaggatgcgg gcgatccgcg
     1981 agctctgggt gacgctgtcg atcagatcct tgccggacat ttcgtaatcg acgccgagcg
     2041 cgacccagcg catcgcccag tcgaccttcc actgcagctt ggcggcgccg gacaggatcg
     2101 actgctcgac ccgctctccc tcatcctcga aggcgatcag gccc
//
LOCUS       DASWNU010000098         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_186210, whole genome shotgun sequence.
ACCESSION   DASWNU010000098 DASWNU010000000
VERSION     DASWNU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186210"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..765)
                     /locus_tag="VGB57_01895"
     CDS             complement(175..765)
                     /locus_tag="VGB57_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0130127.1"
                     /translation="MPRPARQVLPGLPHHATQRGNGRQRIFFGDDDHAFYLSLLGHFC
                     RKSGTAVWAWCLMPNHVHLILVPAHEDGLRATLAPAHTRYAVEINRRQGRCGHLWQGR
                     FASVPMDEAHLHACLRYVELNPVRAGLVERPETWPWSSARAHLGLAPDGLTDLAPMQA
                     RIEDWRAFLDAGLDDEDRDSIRKAERSGRLSPKRRR"
     gene            1061..1777
                     /locus_tag="VGB57_01900"
     CDS             1061..1777
                     /locus_tag="VGB57_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEY0130128.1"
                     /translation="MSVEGERRAGRGSKSERFEMRLDARLIERLDRWRGEQEDKPSRA
                     EAVRRLVEAGLAGGRRAFSIGDGEKLLLAMVSDIYARTVPDGEFDAAFVTSALYGGHH
                     WGLEWGYPGLFHGHVDSPAVVSEVVNILDMWSSVESSFDRLASDARTRVEAAAGQDRA
                     LFRFAGFDGNNESEHLSAARFMIEKLERFPAFKTRELNSHWPMLERYRAQYRAFEPIR
                     ARARGELLRAADLIEIMRER"
     gene            1847..2578
                     /locus_tag="VGB57_01905"
     CDS             1847..2578
                     /locus_tag="VGB57_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130129.1"
                     /translation="MAGKPPGGARADRARRWSARSEETFFEALADTGNVRAAAGRCGF
                     SAGTVYALRRARPDFAERWAKVLAGVPRRPSGSILRYARGGHVKRIRCHSQWSEEAED
                     LFLDVLAATCNVSLAAEEAGVGHTSVYRQRRLRPDFARKWQAVLEQSYDRLDMALIEA
                     ANACVAGEDERGDRPLRAMSVDQAIRLLQLHRASATGAGRRPGWKGGPTRGFDHYRDS
                     IARKIEAIRKARRDGGGGGDDGERG"
     gene            2562..3926
                     /locus_tag="VGB57_01910"
     CDS             2562..3926
                     /locus_tag="VGB57_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY0130130.1"
                     /translation="MASAARPASFREMAEELSPREMELLLGSLTPDEAQAADLDWPGW
                     GHEGQFEPEAGWSTWVVMGGRGFGKTRAGSEWVSGLVRGGPGDSHRGRSPATVPIRIA
                     LVGATLDEVRRVMVEGPSGLLEVAHDQIDDWSPSLRRLRFTGGAEATLFSGASPESLR
                     GPEHDFAWCDELAKWCHAQASWDMLQLGLRRGTRPRALVTTTPRPGPVLKGIMAAPGC
                     VTSGGPSWANPHVPPQRIAEMRARYAGTRLARQELAGELLPDVAGALWTVELLERCRV
                     DPGRGDGHGAGRGRIIIGVDPPAADGTCGIVACGLDPRGQGHVLGDHSVTGRSPEGWA
                     RAVAAAAEAHGAILAVAEANQGGRMVESVLRAASQSLRVKLVHAAEGKSARADPVATL
                     FEAGRVTVHGRLDALESELCGMIAGGGYEGPGRSPDRADAMVWALSELMLQKAGAEPR
                     IRRL"
BASE COUNT          622 a         1240 c         1579 g          598 t
ORIGIN      
        1 ctggtgactg tcactagtga cagtcacttt tcgacgcggg cctagtggcg ggcctagtga
       61 cagtcactag tgactgtcac tttcgactag tgacagtcac tagtgacagt cacttttgac
      121 ctggtgacgg tcactttcga cgaggtcgcg gtggccgtca cgcgggaatg gcgcttagcg
      181 gcggcgcttc gggctgaggc ggccgctgcg ttcggccttg cggatggaat cgcggtcctc
      241 gtcgtcgaga ccggcatcga ggaaggcgcg ccagtcctcg atcctggcct gcatcggcgc
      301 caggtcggtg aggccgtccg gagcgaggcc gagatgggcc cgggcgctcg accagggcca
      361 tgtctcgggt cgctcgacga gaccggcccg aaccgggttc aactcgacgt agcgcaggca
      421 ggcgtgaagg tgcgcctcgt ccatcgggac ggacgcgaag cggccttgcc agagatgtcc
      481 gcatcggccc tggcggcggt tgatctcgac ggcatagcgg gtgtgcgccg gcgccagcgt
      541 cgccctcagg ccgtcttcgt gcgcgggcac caggatgagg tggacgtggt tgggcatcag
      601 gcaccaggcc cagacggccg tcccgctctt ccggcagaaa tggccgagca gcgagaggta
      661 gaaggcgtgg tcgtcgtcgc cgaagaagat tcgctggcgg ccgttgccgc gttgggtggc
      721 gtggtggggc agtccaggca ggacctggcg ggcggggcgc ggcataggcg cagactaacc
      781 ggaaaccgaa atccgcgcca gtccaaacat caaaacgagt gaatggcgag tgagtgcgac
      841 gactgagtga ctgtcactag tgactgtcac ttttcgccca cttttcgccg tcacttttcg
      901 ccgagtgact gtcactagtg actgtcactt tttcgaaatg gtggctgcgg gggggcgtgt
      961 cggggatcgg cgggcaggtg ggtgacggtg gcgatacggc ttgactgtac gtagtgtgtt
     1021 ccgtatattg gtgtcgtaag gtgctgcggg ggtgggtcga atgagcgtgg aaggcgagcg
     1081 cagggcgggg cggggttcga agagcgagcg gttcgagatg cggctcgatg cgcggctgat
     1141 cgagcggctc gaccggtggc gcggggagca ggaggacaag ccgtcgcggg ccgaggcggt
     1201 tcgaaggctc gtcgaggcgg ggctcgcggg cgggcgccgg gcgttttcga tcggcgacgg
     1261 cgagaagctg ctcctggcga tggtttccga catctacgcg aggacggtgc cggacggcga
     1321 attcgatgcc gccttcgtca cctcggcgct ctacggcggc catcattggg gcctcgaatg
     1381 gggttacccc gggctgttcc acggccatgt cgacagcccg gccgtggttt cggaagtcgt
     1441 caacatcctc gacatgtgga gcagcgtcga atcgagcttc gaccggctcg cctccgacgc
     1501 caggacccgg gtggaagcgg cggcaggcca ggaccgagcc ctgttccggt tcgccggctt
     1561 cgacggcaac aacgaatcgg agcatctgag cgcggcccgt ttcatgatcg agaaactgga
     1621 gcggttcccc gccttcaaga ctcgcgagct caattcgcat tggcccatgc tggagcgtta
     1681 ccgggcgcaa taccgggcct tcgaaccgat ccgagcccgg gcgaggggcg agctcctgcg
     1741 ggcggccgac ctgatcgaga taatgcggga gcggtaaggg cggcgagctc acggggaaca
     1801 agctcattgg gaacaaacca tgaacaggaa aagcagggag ggcgagatgg ctggaaaacc
     1861 gcctggcggc gcccgggccg atcgggcgcg gcgctggtcg gcacggtcgg aggagacgtt
     1921 cttcgaagcc ctggcggaca cgggcaacgt ccgggccgcc gccgggcgat gcgggttcag
     1981 cgccggcacg gtctatgcgc ttcggcgggc gcggccggac ttcgccgagc ggtgggcgaa
     2041 ggtgctcgcc ggggtgccgc ggcggccgtc gggaagcatc cttcgctatg cgcgcggcgg
     2101 gcacgtcaag cgcatccgct gccacagcca gtggagcgag gaagcggagg acctcttcct
     2161 cgacgtcctc gccgccacct gcaacgtcag ccttgccgcc gaggaggcgg gagtcggcca
     2221 caccagcgtc taccggcagc gccggcttcg ccccgacttc gcgcgcaaat ggcaggcggt
     2281 gctggagcag agctacgacc ggctcgacat ggcgctgatc gaggcggcca atgcctgtgt
     2341 cgccggcgag gacgagcgcg gcgaccggcc gttgcgggcg atgagcgtgg accaggcgat
     2401 ccggctgctc cagctccacc gcgcctcggc gaccggagcg gggcggaggc cgggctggaa
     2461 agggggcccc acccgggggt tcgatcatta tcgcgacagc atcgcgagga agatcgaggc
     2521 gatccgcaaa gcccggcgcg acggcggcgg cgggggcgac gatggcgagc gcggctaggc
     2581 cggccagctt ccgcgagatg gcggaggaac tcagcccgcg ggaaatggag cttttgctcg
     2641 gctcgctcac tcccgacgag gcgcaggcgg ccgatctcga ctggccgggc tgggggcacg
     2701 aaggccagtt cgagcccgaa gcgggctggt cgacctgggt ggtcatgggc ggacgcggct
     2761 tcggcaagac ccgggcggga tcggaatggg tctccggcct ggtgcgcgga ggccccgggg
     2821 acagtcaccg gggacggtca ccagcgactg tcccgatccg gatagcgctt gtcggggcca
     2881 ctctggacga ggttcgaagg gtgatggtcg aggggccgtc cggattgctc gaggtggcgc
     2941 acgaccagat cgacgactgg tcgcccagcc tgcggcggct tcgcttcacg ggcggcgcgg
     3001 aagcgaccct gttctcgggc gcgtcgcccg aatcgctgcg gggccccgag cacgatttcg
     3061 cctggtgcga cgagctcgcc aaatggtgcc atgcccaggc gagctgggac atgctccagc
     3121 tcgggctgcg gcgggggacc cggccgcggg cgctggtgac gacgactccg cggccggggc
     3181 cggtcctgaa ggggatcatg gcggcaccgg gctgcgtgac cagcggcggc cccagctggg
     3241 cgaacccgca tgtgcccccg caacggatcg ccgagatgcg ggcgcgctat gcgggcaccc
     3301 ggctggcccg gcaggaactg gcgggcgagc ttctgccgga cgtggcgggg gcgctgtgga
     3361 cggtggagtt gctggagcgg tgccgggtcg atccgggccg gggcgacggc catggcgcgg
     3421 gccgcggccg gatcatcatc ggggtcgatc cgccggccgc ggacgggacg tgcgggatcg
     3481 tggcgtgcgg gctggacccg cggggacaag ggcatgtgct cggcgaccac agcgttaccg
     3541 gccgctctcc ggaagggtgg gcccgggcgg tggcggccgc cgccgaggcc cacggcgcca
     3601 tcctcgccgt cgccgaggcc aatcagggcg gaaggatggt cgagagcgtg ctcagggcgg
     3661 cctcgcaatc gctcagggtc aagctcgtcc acgccgccga gggcaaatcg gcgcgtgccg
     3721 atcccgtcgc caccttgttc gaggcgggac gggtgacggt ccacggacgc ctggacgcgc
     3781 tggagtcgga gctgtgcggg atgatcgccg gcggcggcta cgaagggccg gggaggagcc
     3841 cggaccgggc cgacgccatg gtctgggcgc tgagcgaatt gatgctccag aaggcgggcg
     3901 ccgagccccg gatccggcgc ctttgaccga agccgcgggc ggcggcggct atttcccgcc
     3961 gccgccgccg ccgaacgtgt aggtcagggc gaggccgccg gagaattggt cgcgcgatcc
     4021 gaagccgcgc accaccggc
//
LOCUS       DASWNU010000099         7706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_133134, whole genome shotgun sequence.
ACCESSION   DASWNU010000099 DASWNU010000000
VERSION     DASWNU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133134"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGB57_01915"
     CDS             complement(<1..180)
                     /locus_tag="VGB57_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0130131.1"
                     /translation="MTLISVVMPVLDAGPWLGESIGSILTQTHEALELIVVDDGSADD
                     SREVAAAAAAGDDRVR"
     gene            complement(177..1286)
                     /locus_tag="VGB57_01920"
     CDS             complement(177..1286)
                     /locus_tag="VGB57_01920"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEY0130132.1"
                     /translation="MSQAPFYRAAAVQAAAAGASVSLRSPETEEWVVLGGAARLIWER
                     LEYAASAEELAAALGAEFEAPPEVIEDAVAGTLAELKRLGVVAETPGSGRDRIRDRYL
                     SLLKRALGNLLYPELELQIGHLERGADGLSGHELHRFQRDIAERQPDGFAELMAGKRQ
                     GTGPLRYAHTMIGLFRLSNIERCAEQVIAERIPGDFLEAGVCKGGAAIFMRGLQIAHG
                     ASDRKTFVVDSFEGLPPRDRARDADDGLHLHEDRLSWLACGEESVRDHFSRYDLLDSN
                     VEFVKGWLAQSLPGAAIGQLALLRIDVDLYSSTLECLDLLYDKVVEGGFVIVDDYGFL
                     QCCRDAVEEFRSRRGVTEPIRWIDGSGIYWRKEAS"
     gene            complement(1539..3473)
                     /locus_tag="VGB57_01925"
     CDS             complement(1539..3473)
                     /locus_tag="VGB57_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY0130133.1"
                     /translation="MTALAGYWTWGRGEAPLDACARMIEAQRVYGVAPPVCRGDGAMA
                     MGKSLYPTLAEDAYDPGVTIGGGRRWWLVADVRLDDRAGLARSLGMEEEDERRLADGA
                     LVMAAVERWEEEAIGRLAGDFAIALWDRKRERLLLARDPLGQRPLHYHRSGGFFAFAS
                     MPAGLQTLDGVPSGPDEAALASFLALIPPDGNRSYFRGVERVRPGELRTVSAGGDSSR
                     RHWSFAGGRLQLKRDEDYVEAVREALDRAVESRLRGAGGRVAAHLSGGLDSGAVAATA
                     ARLLPEGRVGAFTSVPGQAGFKRRGRFADEGGHAAAVAALYPNMEHVLVRTAGRSPIA
                     GLDRNFDLYQAPVLNLCNFVWGEAIAEAASERGHKVLLTGQAGNFTLSHTGLDLLPGL
                     LAGGRLIRLGRQALALRRGGTRLESVAAHTLWPFLPPRLWRLINRLRGRRVGAGAGEN
                     SLLNPALGESAAKQLADGLAQATAGPDRGLGIRLAVLASMEFGNFRQGALAGWGLDVR
                     DPTGDRRLIETCLSIPAEIYLSGGVSRGLARRALADRLPPLVVDETRKGFQAADWHEG
                     LLADWDSVRREVGRIAAVPAAARLLDTGRMRTLAEAPAIRDWDSDEAHAHYRLALLRG
                     LSAGHFVRRAEGAGGAGPPD"
     gene            complement(3523..4620)
                     /locus_tag="VGB57_01930"
     CDS             complement(3523..4620)
                     /locus_tag="VGB57_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0130134.1"
                     /translation="MTAPTRFRLRRLWFQIHKWIGLALALLIVPISLTGSALVWHHWL
                     DERLNPERAVTAAPSLPASAYAAAARRLARPGERLSSLAYPRHSGPVVATLARTGQPA
                     GGRPVRTLLYLDPASARLLDRAGSDEGAVRVLHVLHGSLMVPGVGRQIVGWIGVAMLL
                     SSLTGLWLWWPLSGRFTRGLKWRRRATTSANLHHQGGFWIALPLAVLSFTGAWISFPA
                     FFTALSGDPAGPSMAERMRRMAALPAAETRLSPDSALAAARPLATGGLVSLTWPGETD
                     PKWKIAFARPGGTAEATVDDGTAAASPPKAPQPETTARLMRRIHDGTGMGALWQTIIF
                     IGGLIPALLAVTGILMWLQVRRGRSRGRVES"
     gene            4689..4979
                     /locus_tag="VGB57_01935"
     CDS             4689..4979
                     /locus_tag="VGB57_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HEY0130135.1"
                     /translation="MIETTAAVPTSHGAKYVKQLCNHWAHKLEVEQSDGAGTVRFPAA
                     AATMRAGEQELVVTIGAEDEATLERMKSVLATHLDRFAFREAPLPFDWQPGR"
     gene            complement(4981..6255)
                     /locus_tag="VGB57_01940"
     CDS             complement(4981..6255)
                     /locus_tag="VGB57_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY0130136.1"
                     /translation="MNASRSEQIVVVGLGYVGLPLAVALARSFDVLGLDIDERRVEEL
                     RLGVDRTHEVESEALAASPLRLTSRSADAAGADIYIVTVPTPVDGDNSPDLRPVLSAT
                     RSLATLLDPARKPIIVYESTVYPGVTEEVCGPLIEEISGLKRGLDFFLGYSPERINPG
                     DREHTVDRIIKVVAGENEAVTERLAAIYGAVTSAGVFRAASIKTAEAAKVIENAQRDI
                     NIAFMNEITKIFSKLDISIWDVLAAAGTKWNFLPFQPGLVGGHCIGVDPYYLSFRAQQ
                     LGLDPHVILAGRSTNDGMGAWVADGLHNRLGRPGRALIMGLAFKENVPDLRNSRVIDV
                     IRRLQELGHEVTVHDPLADTAEARHEYGIDLADDAFDGSYDLVLVAVPHAAYSELDDS
                     SLAGLVAEGGLLADLKHLYKTRELPGVERWTL"
     gene            complement(6332..6937)
                     /locus_tag="VGB57_01945"
     CDS             complement(6332..6937)
                     /locus_tag="VGB57_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130137.1"
                     /translation="MILSFRLPRVTAFVAAALLALTAAPGWAAVSITFYSKELGASFP
                     HAFVILEGTLDRGGPRIEEDYGFTAKTISPAILWGKVAGEVITDHSVGYVKGSDRHFT
                     VTLADAEYDRVMATIARWRSAKQPSYDLDKHNCVHFVADLAAAIGMTPDTERLMKKPR
                     SFLETLTARNRPWLTGRNATFYRAGPATIPKRSGPKVVPRA"
     gene            7224..7481
                     /locus_tag="VGB57_01950"
     CDS             7224..7481
                     /locus_tag="VGB57_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130138.1"
                     /translation="MAFKGLIAAAASVALMTTPTLAAAQASAGAARAATEVAPATETV
                     EGDQLRGGWIIPLIALIAIILGICAATDVCGGDDDDQPTSP"
     gene            complement(7496..>7706)
                     /locus_tag="VGB57_01955"
     CDS             complement(7496..>7706)
                     /locus_tag="VGB57_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130139.1"
                     /translation="PERQDPQLVQRHERSGPACCTKATVRRELPEMASVSEVSQVRPD
                     AAAGVQTQKWPDEGELRPAYFNLSE"
BASE COUNT         1252 a         2641 c         2662 g         1151 t
ORIGIN      
        1 cctcaccctg tcgtcgcccg ccgcggccgc cgccgcgacc tcgcggctgt cgtcggccga
       61 cccgtcgtcg acgacgatca gctccagcgc ctcgtgggtc tgggtgagga tcgacccgat
      121 cgattccccg agccacggcc cggcgtcgag cacgggcatg acgaccgata tcagggtcac
      181 gacgcttcct tgcgccagta tattccggag ccgtctatcc accggatcgg ctcggtcact
      241 ccgcgcctgg agcggaactc ttcgacggcg tcgcgacagc attgcaggaa accgtaatcg
      301 tcgacgatga cgaacccgcc ctcgacgacc ttgtcgtaga ggaggtcgag gcattccagg
      361 gtggagctgt aaaggtcgac gtcgatccga agcaaggcga gctggccaat cgccgcgccc
      421 ggcagggact gggccagcca gcccttcacg aactcgacgt tggaatcgag cagatcgtag
      481 cgcgaaaaat gatcccgcac cgactcctcg ccgcacgcca gccaggaaag ccggtcttcg
      541 tggagatgaa ggccgtcatc ggcgtcccgg gcccgatcgc gcgggggcag gccctcgaaa
      601 gaatcgacca cgaaggtttt ccggtcgctc gcgccatggg cgatctgcag cccgcgcatg
      661 aagatcgccg cgccgccctt gcacacgccg gcctcgagaa agtcgccggg gatgcgctcg
      721 gcgatgacct gctccgcgca gcgctcgatg ttggagaggc ggaacaggcc gatcatcgtg
      781 tgggcgtagc gaagcgggcc ggtgccctgg cgctttcccg ccatcagctc ggcgaagccg
      841 tccggctgcc gctcggcaat gtcgcgctgg aagcggtgaa gttcgtggcc tgaaagaccg
      901 tcggcgccgc gctccagatg cccgatctga agctccagct ccggatagag caaattgccg
      961 agcgcccgct tcagcagcga caaatagcgg tctcgaatcc tgtcgcggcc tgagcccggg
     1021 gtctccgcga cgacgccgag gcgcttaagc tcggcgaggg ttccggccac cgcgtcttct
     1081 atcacctccg gcggagcttc gaactcggcg ccaagcgccg ccgccaattc ctcggcgctc
     1141 gcggcatatt ccagcctctc ccagataagc cgggcggcgc cgccgagcac gacccactcc
     1201 tcggtctcgg gagaacggag cgagacggag gctcccgccg ccgccgcctg gacggccgcg
     1261 gccctgtaga agggggcctg gctcaacgtg cgctctggcg gtgcggaatg gcttccgcgg
     1321 cgcgatcagg aatagaggcc gtagtcgacg ccaccgatag gcccgccgaa ggcgttggcg
     1381 ccgcgggcgg gggcaatctc gatttcagga gcaatccact gccgcttacc cgaactcgac
     1441 gggctctggg cgctaccgcc acgtactttg tcagtcacaa gccctcctgg aaccacggca
     1501 agccccgtcg ttaggaggtt cgccggcccg aggcaacctc agtcgggcgg gccggcacct
     1561 ccggcgccct ccgcgcggcg cacgaaatgc ccggcggaga gccccctgag cagggccagc
     1621 cggtagtgcg catgggcttc gtccgagtcc cagtcgcgta tcgccggagc ttcggcgagg
     1681 gtcctcatcc tgcccgtatc gagcagcctg gccgccgccg gaaccgcggc gatccggccg
     1741 acttcgcgcc ggacggaatc ccagtcggcg agcagcccct cgtgccagtc cgcggcctgg
     1801 aagcccttgc gggtctcgtc gacgacgagc ggcggaagcc ggtcggccag cgctcgtcgc
     1861 gccagcccgc gcgacactcc gccggacaaa tagatttcgg cggggatgga caggcaggtt
     1921 tcgatcagcc gccggtcgcc ggtcgggtcg cgcacgtcca ggccccagcc ggccagggcg
     1981 ccctgccgga aattgccaaa ttccattgaa gccaggaccg cgagccggat cccgagcccc
     2041 cggtcgggcc ccgccgtcgc ctgcgccagc ccgtccgcca attgtttcgc ggcggactcg
     2101 cccagggccg ggttgagcag gctgttttcg ccggccccag cgccgacacg gcggccgcgc
     2161 aggcgattga tcagccgcca caggcgcggc ggcaggaacg gccacaaggt gtgagcggcg
     2221 acgctttcaa ggcgggtgcc gccgcgccgg agggcgagcg cctgcctgcc gagccggatc
     2281 agccttccgc cggcgagcag cccgggcagc aggtcgagcc cggtgtggct gagcgtgaag
     2341 ttgccggcct ggccggtgag caggaccttg tggccgcgct cgctcgcggc ctcggcgatc
     2401 gcctcgcccc agacgaagtt gcaaaggttg agcaccggcg cctggtacaa gtcgaaattg
     2461 cggtccaggc cggcgatcgg cgagcggccc gcggtccgga cgaggacatg ctccatgttc
     2521 ggatataaag cggcgaccgc cgccgcatgc ccgccctcgt cggcgaagcg gcccctgcgc
     2581 ttgaagcccg cctgccctgg aaccgaggtg aaggcgccga cccgtccctc gggcaggagc
     2641 cgcgccgccg tcgccgccac cgctccgctg tcgaggcccc cgctgagatg cgccgcgacc
     2701 cgtccgccgg cgccgcgcag ccgcgattcg accgcccggt cgagcgcttc gcgcaccgcc
     2761 tcgacataat cctcgtcgcg cttcagctgc agccggccgc cggcgaacga ccagtggcgg
     2821 cgcgagctgt cgccgccggc cgagacggtc cgcagctcgc ccggccggac tcgctcgact
     2881 cctcggaagt agctgcgatt gccgtccggc gggatcagcg ccaggaagga ggcgagagcc
     2941 gcctcgtccg ggcccgaagg aacgccgtcg agcgtctgga ggcccgccgg catcgatgcg
     3001 aaggcgaaga agccgcccga ccggtggtag tggagtggcc gctgccccag cggatcccgg
     3061 gcgaggagca gccgctcgcg cttccggtcc cacaaggcga tggcgaagtc gccagccagc
     3121 cggccgatcg cctcctcctc ccaccgctcc accgcggcca tgaccagtgc gccgtcggcc
     3181 agccgccgct catcctcctc ctccatgccg agcgagcggg cgagccccgc gcggtcgtcg
     3241 agccggacgt cggcgaccag ccaccaccgg cggccgccgc cgatcgtcac tcccgggtca
     3301 taagcatctt cggcaagcgt cgggtacagg ctcttgccca tcgccatcgc gccgtcgccg
     3361 cggcagacgg gcggagcgac tccatagacg cgctgcgcct cgatcatgcg ggcgcaggca
     3421 tcgagcggcg cctcgccccg gccccatgtc caatagcccg caagcgccgt catcccgcga
     3481 cgcctagctc ccgcaccgct ccttctcaac ccgaaggtcg gatcaggact cgacccggcc
     3541 tcgcgacctt ccgcgccgga cctgaagcca catcaggatt ccggtgacgg cgagcagcgc
     3601 cgggatgagc ccgccgatga agatgatcgt ctgccacagc gcgcccatgc cggtgccgtc
     3661 gtggatgcgc cgcatcagcc tagccgttgt ctcgggctgc ggcgccttgg gcgggctcgc
     3721 cgcggcggtg ccgtcgtcga ccgtcgcctc ggccgttcct cccggccggg cgaaggcgat
     3781 cttccacttc gggtcggttt cgccgggcca ggtcaggctg accaggccgc cggtcgccag
     3841 cggtcgcgcc gccgccagcg ccgaatcggg actcagcctc gtctcggccg ccggcagcgc
     3901 cgccatgcgc cgcatccgtt ccgccatgga cggacccgcc ggatcgcccg aaagcgcggt
     3961 gaagaaggcc ggaaaggaga tccaggctcc ggtgaaggag aggacggcca ggggcaaggc
     4021 gatccagaag ccgccctggt ggtggagatt ggcgctggtc gtcgctcgcc ggcgccactt
     4081 gaggccgcgg gtgaagcggc cgctgagcgg ccaccacagc cacagtccgg tcagcgacga
     4141 aagcagcatc gccaccccga tccagccgac gatctgccgg ccgacgcccg gcaccatcag
     4201 cgagccgtgg aggacgtgca ggacgcgcac cgcgccttcg tcgctgccgg cgcggtcgag
     4261 caggcgcgcc gaggcggggt cgaggtagag cagggtgcgg accggtcggc cgccggccgg
     4321 ctgcccggtc ctcgccagcg tcgcgacgac cggtccggag tgccgcggat aagcgaggct
     4381 cgacaaccgc tcgcccggcc gcgcgaggcg ccttgccgcg gcggcatagg cggaagccgg
     4441 aaggctcggc gccgccgtca cggcccgctc cgggttgagc ctttcgtcca gccagtggtg
     4501 ccagaccagg gccgagccgg tcaacgagat cggcacgatc agcaaggcga gggcgaggcc
     4561 gatccatttg tggatttgga accagaggcg gcggaggcgg aatcgggtcg gggcggtcat
     4621 ggtgacgggc taagtcactt gcgaatgatt atcaatagca tatatagctg cgacgagagg
     4681 attccacgat gatcgagact accgccgccg ttcccaccag ccacggcgcc aaatatgtga
     4741 agcagctgtg caaccattgg gcgcacaagc tggaggtgga gcagtcggac ggagccggga
     4801 cggtgcgttt cccggccgcg gccgcgacca tgcgcgccgg ggagcaagaa ctcgtcgtca
     4861 ccatcggggc cgaggacgaa gcgactctcg aacggatgaa atccgtcctc gccacccatc
     4921 tcgaccgatt cgcctttcgc gaagccccgc tcccgttcga ctggcagccg ggccgctgag
     4981 ctagagcgtc cagcgctcca cgccggggag ttcgcgggtc ttgtagaggt gcttgaggtc
     5041 ggcgaggagg ccgccctccg ccaccaggcc cgcaagactc gaatcgtcca gttccgaata
     5101 agcggcgtga gggacggcga cgaggacgag gtcgtagctc ccgtcgaacg catcgtcggc
     5161 aaggtcgatt ccatattcgt gacgggcctc ggcggtgtcg gcgagggggt cgtgcaccgt
     5221 cacctcatgg ccgagctcct ggagccggcg gatgacgtcg atcacccggc tgttcctcag
     5281 atccggtacg ttttccttga aggccaggcc catgatcagg gcccggcccg gccgtccgag
     5341 cctgttgtgc aggccgtccg ctacccaggc gcccatgccg tcgttggtcg agcggccggc
     5401 gaggatgacg tgcggatcga ggccgagctg ctgggcccgg aagctgagat aataaggatc
     5461 gacgccgatg cagtggccgc cgacgagccc gggctggaag ggcaggaagt tccacttggt
     5521 gccggcggcg gcgagcacgt cccagatcga gatgtcgagc ttcgagaaga tcttggtgat
     5581 ctcgttcatg aaggcgatgt tgatgtcgcg ctgggcgttc tcgatcacct tggccgcctc
     5641 ggccgtcttg atcgaggcgg cccggaagac cccggcgctg gtcaccgccc cgtagatcgc
     5701 cgccagccgc tcggtcaccg cctcgttctc gccggcgacg accttgatga tccggtcgac
     5761 cgtgtgctcg cggtcgcccg gattgatccg ctcgggggag tagccgagga agaagtcgag
     5821 gccgcgcttg agcccggaga tttcctcgat cagcggcccg caaacctcct cggtcacccc
     5881 cggatagacg gtgctctcgt agacgatgat cggcttgcgc gccgggtcga gcagggtggc
     5941 gaggcttcgg gtggcgctga gcaccgggcg gagatcgggg ctgttgtcgc cgtcgaccgg
     6001 ggtcggcact gtgacgatgt aaatgtcggc gccggcggcg tcggcggagc ggctggtgag
     6061 ccgcagcggc gaggcggcga gcgcctcgct ctcgacctcg tgggtgcggt cgacgccgag
     6121 cctgagctcc tcgacgcgcc tttcgtctat gtcgagaccg aggacgtcga agctgcgtgc
     6181 tagggcgacc gcgagcggga ggccgacata gccgaggccg acgaccacga tctgctcgga
     6241 gcgggacgcg ttcattcgag gatcactctg gaacaggagg gcggcgggat cgccggtaca
     6301 ggggcgctaa cattagaggg tgaaagaaat gtcaagcgcg ggggacgacc ttcggaccgg
     6361 agcgtttggg gattgtcgcg gggccggcgc gatagaaggt cgcgttgcgg ccggtcagcc
     6421 agggccggtt ccgggcggtg agggtctcaa ggaacgagcg gggcttcttc atcagcctct
     6481 ccgtgtcggg cgtcattccg atggcggcgg cgagatcggc gacgaagtgg acgcaattgt
     6541 gcttgtcgag atcgtagctg ggctgcttcg ccgagcgcca gcgcgcgatc gtcgccatca
     6601 cccggtcata ttcggcgtcg gcgagagtga cggtgaaatg gcggtcgctg cccttgacgt
     6661 aaccgacgct atgatcggtg atcacctcgc cggcgacctt gccccacagg atggcggggc
     6721 tgatcgtctt ggcggtgaag ccgtaatcct cctcgattcg cggcccgccg cggtcgagcg
     6781 tcccttcgag gatgacgaag gcgtggggga agctcgctcc caattccttc gaatagaagg
     6841 tgatcgacac agccgcccat ccgggcgcgg cggtcagggc gagcaaggcc gcggccacaa
     6901 aggcggtcac gcgcggcaga cgaaagctca ggatcaccgg ctcctcccaa agtcgggttc
     6961 gcgaaccctc tgaaacaaaa agccatttcg agcaatcccc agcttagtgt cgcacgcgtg
     7021 caacagtgcc ggcgagttcc gccgccgcgc ccgatccttt gccctccgac ccttcgctcc
     7081 agcctgcgta aacccttgag ggaacagggc ctttccttct ttcggggacg tccaactttc
     7141 gggatagaca cgccgaagcg cctcgactag ataaagtatc gaaatcgaga cgatcagact
     7201 cgggtacgaa gggagaagct gacgtggctt tcaagggact tattgctgcg gccgcctcgg
     7261 tggccttgat gacgacgccg actctggcgg cggcgcaggc gagcgcgggt gcggcccgtg
     7321 cggccaccga agtggcgccg gcgacggaga ccgtcgaggg cgaccagctg cgcggcggct
     7381 ggatcattcc tctgatcgcg ctcatcgcga tcatcctcgg catctgcgcc gcgaccgacg
     7441 tgtgcggtgg cgacgacgac gatcagccca ccagcccgta agctgaagcc gaacgttatt
     7501 cactaagatt gaaataggcc ggacgaagct cgccttcgtc cggccatttc tgcgtctgca
     7561 cccccgccgc cgcgtcgggc cggacctgcg aaacctctga aacagaagcc atttcgggca
     7621 attctcggcg gactgtcgct ttcgtgcaac aggcaggtcc cgatctctcg tggcgttgaa
     7681 ccaattgagg gtcctggcgt tcgggt
//
LOCUS       DASWNU010000100         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_263077, whole genome shotgun sequence.
ACCESSION   DASWNU010000100 DASWNU010000000
VERSION     DASWNU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263077"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="crtY"
                     /locus_tag="VGB57_01960"
     CDS             complement(<1..925)
                     /gene="crtY"
                     /locus_tag="VGB57_01960"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HEY0130140.1"
                     /translation="MTAPAPKLIIAGGGLAGCLAALALAERRPDVDFLIVEGEERFGG
                     NHVWSFFDGDVAGPDRWLIDPLVGHRWSDHEVRFPNRFRTLGFGYNSVRSADLDARMR
                     STLAAGRYRLGTRIAEIAPNQVTLASGERLAAAAVIDARGAGPNPKLDLAWQKFVGLT
                     CRFERDHGVERPIIMDSCVEQDDGYRFVYSLPFSPTELLVEDTYYSTSPVLDVPLVRC
                     RVAGQMRRRGLEPSEILAQEAGVLPILLGGRLEDLWSAADPPVARLGLRGGFFHPTTG
                     YSLPDAVRNAILLTEQPELTSDALHAIFRRRS"
     gene            988..1416
                     /locus_tag="VGB57_01965"
     CDS             988..1416
                     /locus_tag="VGB57_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEY0130141.1"
                     /translation="MPRRLLLAPLLLILVAADRPTADIEISIEKLRNRKGVLHLCLTR
                     EPAHFPDCKKDPRAVTRSAPATTGPIRITGLAPGGYAVAIFHDENRNKKLDVLIGIPR
                     EGFGFSRNPVVRFGAPKFRQVLIDLRPGFTRETVRMQYLL"
     gene            1481..2440
                     /gene="ispH"
                     /locus_tag="VGB57_01970"
     CDS             1481..2440
                     /gene="ispH"
                     /locus_tag="VGB57_01970"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0130142.1"
                     /translation="MIDSGARTGFPPLDILLAAPRGFCAGVSRAIQAVRDALRVHGTP
                     IYVRRPIVHNLRVVRALEAEGAVFVEELDEVPEGAVVILSAHGVEPAVREEAAERGLI
                     AYDAVCPLVAKVHREVERHHRTGRHVLLLGHDGHPEIVGTLGHAPAGAVSVIRGAADV
                     HALPFPAGRRLALAVQTTYSVDEAEEVIAALDRRFPDVARPAAGDICYATTNRQAAVR
                     AIAARADAMIVAGESFSSNACRLAELAVAAGCSAVQRIAGPEELDWSRLPRNGSLGIT
                     AAASTPESVVTDIIDALRASYRVRIEEVRAVEENTQFRRVAIG"
     gene            2443..3348
                     /locus_tag="VGB57_01975"
     CDS             2443..3348
                     /locus_tag="VGB57_01975"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY0130143.1"
                     /translation="MIDSVRAAVVLGPSMRRAAADIDALFEAILPDPPDPRRRLYQAM
                     RYAAVGSGKRLRPLLTIAASGLFDVARESAVRAGLAVECVHVHSLIHDDLPCMDDDDL
                     RRGKPTVHRAYDEATAVLAGDALLAMAFEILGDPAMPVSGDIRAELVCELARAAGAGG
                     MAGGQMLDLFPTEALDLDAITRLQRLKTGALIGWCVEAGAIMGQASADGRTSLRGYAH
                     CLGLAFQIADDLLDHNGDEARVGKKLRKDDDQGKVTFVTMLGVDRARRQARLLADQAI
                     EHLQGFGRDADLLAAIARFAVSRDH"
     gene            complement(3332..3976)
                     /locus_tag="VGB57_01980"
     CDS             complement(3332..3976)
                     /locus_tag="VGB57_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0130144.1"
                     /translation="MTAFGSIDRLEGTEGLLASIDHSPIASVITDARLPDNPIVAVNR
                     GFCELTGYEREEIVGRNCRFLAGPDTEPDARSRLGRAVAEGRPALVELTNYRKDGSAF
                     LNAVMIAPVIGEDSEVAYFLGSQMDVGAGSGLSAPRRQRAEALVRALTPRQLQVLEHM
                     IAGYRNKQIAGFLAIDEKTVKMHRASLLTRLGIGSSAEAIRIGVEAGVRLSGRG"
     gene            4115..4441
                     /locus_tag="VGB57_01985"
     CDS             4115..4441
                     /locus_tag="VGB57_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEY0130145.1"
                     /translation="MNESPSATPTRDPDALTADLCAALLTPADASEMERLLLDLCTPA
                     EIRTLAERWHVARLLYGTGLSYREIHDATGVSTTTVVRVARFLREERNGGYRRAIDSL
                     GGGDAR"
     gene            4431..>5212
                     /gene="hisG"
                     /locus_tag="VGB57_01990"
     CDS             4431..>5212
                     /gene="hisG"
                     /locus_tag="VGB57_01990"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0130146.1"
                     /translation="MLVDETRLHIAIQKSGRLSDYSLGLLKDSGLRIQKGRNALTARV
                     ENFPADLMLVRDDDIPTFVGDGVCEFGIVGENVLEEFALGEAVPRIEVVAPLGFGRCT
                     LKLAAPESAAYAGPASLQGARIATTYPRIVQRFLDRNHVSATVVKMNGAVELAPRLQI
                     APFICDLVSTGATLEANGLRAVETVFDSEAVLIRTRKPVSEAKTEQAERLLSRIEGVL
                     ATKESKYIMLNAPGEALREITALLPGAEAPTILPLHGRAGHFA"
BASE COUNT          811 a         1853 c         1745 g          803 t
ORIGIN      
        1 ccgagcgccg ccggaagatg gcgtgaaggg cgtcgctcgt cagctcgggt tgctcggtta
       61 ggagaatggc attgcggacg gcgtcgggga gggaatagcc ggtcgtcgga tggaagaagc
      121 cgccgcgcag gccgagccgc gccaccggcg gatcggcggc gctccacagg tcctccagcc
      181 ggccaccgag caggatcggc aggactccgg cctcctgagc caggatttcg gacggctcga
      241 ggccacggcg gcgcatctgg cccgccaccc ggcagcgcac gagcgggacg tcgaggacgg
      301 gcgaggtcga gtaataggta tcctcgacca gcaattcggt agggctgaag ggcaggctgt
      361 agacgaagcg atagccgtcg tcctgctcga cgcacgagtc catgatgatc ggtcgctcga
      421 cgccgtggtc gcgctcgaag cggcaggtca gtccgacgaa cttctgccac gccagatcga
      481 gcttagggtt cggccctgcc ccgcgcgcgt cgatcaccgc cgcggccgcg aggcgctcgc
      541 cgctggccag ggtgacctgg ttcggcgcga tttcggcgat ccgggtgccg agccggtagc
      601 gcccggcggc cagggtggag cgcatgcgcg catccagatc ggccgacctc acgctgttat
      661 agccgaagcc cagggtccgg aaccggttcg ggaagcgcac ttcgtgatcg ctccagcgat
      721 gcccgaccag cgggtcgatc agccaccggt cggggccggc gacgtccccg tcgaagaagg
      781 accagacgtg gtttccgccg aagcgctcct ccccttcgac gatgaggaaa tcgacgtccg
      841 ggcgccgctc cgccaaggcg agcgcggcga ggcagccggc gaggccgccg ccggcgatga
      901 tcagcttcgg ggcaggcgcg gtcaccaccg acccccgccc aaagaccata tggcggcgcc
      961 gcactggccc gggtagcgcg gtgcgctatg cccagacgcc ttctcctcgc gccgctcctt
     1021 ctcatcctcg tcgccgccga tcgcccgacc gccgatatcg agatttcgat cgagaagctg
     1081 cgcaaccgca agggggtgct gcacctctgc ctgacccgcg agccggcgca cttccccgac
     1141 tgcaagaaag accctcgggc ggtcacgcgc agcgcgcccg ccacgacggg ccccatccgg
     1201 atcaccggcc tcgcgcccgg cggctatgcc gttgcgatct ttcatgacga gaaccgcaac
     1261 aagaagctgg acgtgctgat cggcatcccg cgcgagggct tcggcttttc gcggaatccg
     1321 gtggtgcgct tcggggcgcc gaagttccgg caggtgctca tcgatctgag gccgggcttc
     1381 actcgcgaga ccgtccgcat gcaatacctg ctttagtcca tgtggattcg ccctgcccgc
     1441 ccccgtaagt ttcgcggcgg gggcgacgag caaggatgcg atgatcgact ctggcgcccg
     1501 tacaggcttt cctcccctcg acattttgct cgccgcgccc cgcggcttct gcgcgggcgt
     1561 cagccgggcg atccaggcgg tgcgcgacgc gctgagggtc catggcacgc cgatctatgt
     1621 gcgccggccc atcgtccaca atctgcgggt ggtgagggcg ctggaggcgg agggcgccgt
     1681 cttcgtcgaa gagctggacg aggtccccga aggcgcggtc gtcatcctct cggcgcacgg
     1741 ggtcgagcct gcggtgcgcg aggaagcggc ggagcgcggc ctgatcgcct acgatgccgt
     1801 ctgcccgctc gtcgccaagg tccaccgcga ggtggaacgc catcaccgca ccggccgcca
     1861 cgtcctcctc ctcgggcatg acgggcatcc ggagatcgtc ggaacgctcg gtcatgcgcc
     1921 ggccggagcc gtctcggtga tccgcggcgc cgccgacgtc catgccctgc ccttcccggc
     1981 cggcagacgg ctcgcactgg ccgtccagac cacttactcg gtcgacgagg cggaggaggt
     2041 gatcgcggcg ctcgaccggc gctttccgga tgtcgccagg cccgccgccg gcgacatctg
     2101 ctacgccacg accaaccggc aggcggcggt ccgcgccatc gccgcgcgcg ccgacgccat
     2161 gatcgtcgcc ggcgagagct tctcctccaa tgcctgccga ctagccgagc tcgccgtcgc
     2221 cgccggctgc tccgcggtcc agcgcatcgc cggtcccgag gagctcgact ggtcgcggct
     2281 cccgcgcaac gggtcgctcg ggatcaccgc ggcggcctcg actccggaaa gtgtcgtcac
     2341 cgacatcatc gacgccctgc gggcgagtta tcgcgttcgg atcgaggaag tgcgggcggt
     2401 cgaggaaaat acccaattca ggcgggtggc gatcggatga cgatgatcga cagcgtccgg
     2461 gccgcggtcg tcctcggccc ctccatgcgg cgcgcggccg ccgacatcga cgccctgttc
     2521 gaggcgatcc tgcccgatcc gcccgacccg cgccgccgcc tctaccaggc gatgcgctac
     2581 gccgccgtcg gcagtggcaa gaggctccgt ccgctcctca ccatcgccgc ctccgggctg
     2641 ttcgacgtgg cgcgtgagag cgccgtccgg gcaggcctcg cggttgaatg cgtccacgtc
     2701 cattcgctga tccacgacga tctcccctgc atggacgacg acgatctgcg ccggggcaag
     2761 ccgaccgtcc accgcgccta tgacgaggcc acggcggtgc tcgccggcga tgccctgctg
     2821 gccatggctt tcgagatatt gggcgatccg gcgatgcccg tctccggcga tatccgggcc
     2881 gagctggtct gtgagctcgc gcgggccgcg ggcgccggcg gaatggcggg cgggcagatg
     2941 ctcgacctgt tccccacaga agcgctcgac ctcgatgcca tcacccgcct ccagcgcctc
     3001 aagaccggag ccctgatcgg ctggtgcgtc gaggccggag cgatcatggg ccaggcctcg
     3061 gccgacggtc ggacgagcct tcgcggctac gcccattgcc tcggcctcgc cttccagatc
     3121 gccgacgatc tgctcgacca taacggggac gaagcccggg tgggcaagaa gcttcgcaag
     3181 gacgatgatc agggcaaggt caccttcgtc accatgctcg gggtcgaccg ggcccgccgc
     3241 caggcacggc tgctcgccga tcaggcgatc gagcatctcc agggcttcgg ccgcgacgcc
     3301 gatctgctcg cggcgatcgc ccgcttcgcg gtcagccgcg accactgagc cggactccgg
     3361 cctccacccc gatccggatc gcttcggcgg agctgccgat gccgagcctc gtcagaaggc
     3421 tggcgcgatg catcttcacg gtcttctcgt cgatggcgag gaagccggcg atctgcttgt
     3481 tgcgatagcc cgcgatcata tgctccagca cctggagctg gcgcggggta agcgctcgaa
     3541 ccagcgcctc ggcccgctgc cggcgcggag cggagagccc ggagccggcg ccgacgtcca
     3601 tttgcgagcc gaggaaatag gcgacttcgc tgtcctcgcc gatcaccggc gcgatcatca
     3661 ccgcattgag gaaagcgctt ccgtctttcc gataattggt aagctcgacg agggcgggcc
     3721 gcccttccgc caccgcgcgt ccaagcctcg aacgggcgtc cggctcggta tcgggaccgg
     3781 ccaggaaccg gcagttgcgg ccgacgattt cctcccgctc atagccggtc aattcgcaga
     3841 agccgcgatt gaccgccacg atcggattgt ccggcaatcg ggcgtcggtg atcaccgagg
     3901 cgatcgggct gtggtcgatc gaggcaagca gcccctcggt tccctccagc ctgtctattg
     3961 agccgaatgc tgtcacaggt gcctctctag cattaccggc gccgcctgaa aaatcccggc
     4021 aagtcaatgc ccggcggcgg caatagcggg cttgcgccgc cagccccggc gggctaggtg
     4081 cgtaccagcg tattaatacg ttggaacaaa gatgatgaac gaaagccctt ccgcgacgcc
     4141 gacccgggac ccggacgccc tcaccgccga cctgtgcgcg gcgctgctga cgcccgcgga
     4201 cgcttccgag atggagcggc tgttgctcga tctgtgcacc ccggccgaga tccggacgct
     4261 cgccgagcgc tggcacgtcg cccggctgct ctacggcacc ggcctctcct accgcgaaat
     4321 ccacgacgct accggagtca gcacgacgac cgtcgttcgg gtcgcgcgct tccttcggga
     4381 ggagcgcaac ggcggctaca ggcgggcgat cgactcgctg ggcggcggcg atgctcgttg
     4441 acgagacccg actccacatc gccatccaga aatccggccg gctctccgac tacagcctcg
     4501 gcctgctcaa ggattcgggc cttcgaatcc agaaaggccg caacgcgctt accgcgcggg
     4561 tggagaattt cccggcggac ctgatgctcg tgcgcgacga cgacatcccg accttcgtcg
     4621 gcgacggggt ctgcgagttc gggatcgtcg gcgagaacgt cctcgaggaa ttcgcgctcg
     4681 gcgaggccgt gccgcggata gaggtcgtcg caccgctcgg cttcggccgc tgcaccctga
     4741 agctggcggc gcccgaatcc gccgcttatg cggggccggc ttcgctgcag ggcgcgcgca
     4801 tcgcgaccac ttatccccgg atcgtccagc gtttcctgga ccgcaaccat gtctcggcga
     4861 cggtcgtcaa gatgaacggt gccgtcgagc tggcgccgcg gcttcagatc gcgcccttca
     4921 tctgcgacct cgtctccacc ggcgccaccc tcgaggcgaa cggccttcga gcggtcgaga
     4981 ccgtgttcga cagcgaggcg gttctgatcc gcaccaggaa acccgtttcg gaggcgaaga
     5041 ccgagcaggc cgaaaggctg ctcagccgga tcgaaggcgt tctcgccacc aaggagtcca
     5101 aatacatcat gctcaacgcg ccgggcgagg cgctgaggga gatcaccgcc ctcctccccg
     5161 gcgccgaggc gcccacgatc cttccgctcc acggacgggc cggccatttc gc
//
LOCUS       DASWNU010000101         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_211869, whole genome shotgun sequence.
ACCESSION   DASWNU010000101 DASWNU010000000
VERSION     DASWNU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211869"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VGB57_01995"
     CDS             <1..622
                     /locus_tag="VGB57_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0130147.1"
                     /translation="ETKRIVEAEGRRAVAIPGDVGDKDFCERAVSQTVKELGQLDILV
                     NNAAEQHPDKEITEITEEQLKRTFQTNFYGYFFMVQAARPHLKKGAAIVNCTSVTMYK
                     GSEKLLDYSATKGAITAFTRSLSQNLIEDGIRVNAVAPGPIWTPLNPFGGQPPEEIPD
                     FGKDVPMGRPGQPNEVAPSFLFLACEDSSYMAGQVLHPNGGEVVNG"
     gene            726..1085
                     /locus_tag="VGB57_02000"
     CDS             726..1085
                     /locus_tag="VGB57_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY0130148.1"
                     /translation="MTRYTSFRDFWPHYLREHKRPATRRLHYAGTGLVLGFAVAAAAS
                     RDWRLLAAVPLAGYGFAWFSHAAVERNRPATFTYPLWSLAADFRMAWLWATGRLDSEL
                     REAGVEPERRKGEGDAL"
     gene            1075..1830
                     /locus_tag="VGB57_02005"
     CDS             1075..1830
                     /locus_tag="VGB57_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0130149.1"
                     /translation="MRSEMTDADAGAAIAGLLLKLRARDLVGREEEDVLRASVSELQE
                     HPAGRTIVRTGTTLSASTLLVEGIVCRFKDLADGQRQIMELHVGGDFVDLHGFLLKQL
                     DHNVGAMTPVRVASVPHDALRGITETHPHLGRMLWFSTLLDAAIHREKILSIGRRSAL
                     ARIAHIFCELLVRLRIVGLADDNGYALPLTQADLADSTGLTSVHVNRMLKKLRDENLL
                     TFRGGEVAIGDWERLQRVAEFDPTYLHLERRPR"
     gene            complement(1974..2405)
                     /locus_tag="VGB57_02010"
     CDS             complement(1974..2405)
                     /locus_tag="VGB57_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY0130150.1"
                     /translation="MTLFTIGYEGKTQAEFLDELQAAGVELILDVRAVASSRRPGFSK
                     TALAGGLRERGIDYLHLRSLGTPADGRQAARAGRIAEMRAIYAVQLETPEAGLAMEQA
                     LAEAAARPSALLCYERGAPDCHRSMLAQRMVERVPLEVRDL"
     gene            2504..2722
                     /gene="infA"
                     /locus_tag="VGB57_02015"
     CDS             2504..2722
                     /gene="infA"
                     /locus_tag="VGB57_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0130151.1"
                     /translation="MAKEELLEMRGQVVELLPNAMFRVKLENNHEILGHTAGKMRKNR
                     IRVLVGDEVLVELTPYDLTKGRITYRFK"
     gene            2737..3303
                     /locus_tag="VGB57_02020"
     CDS             2737..3303
                     /locus_tag="VGB57_02020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY0130152.1"
                     /translation="MRLILASASPRRLELLARLGVIPDAVIPAEIDETPRKAEIPDRY
                     ACRMATEKAAAWAEPGSLILAADTVVAAGRRILPKTESEDEARAALALLSGRRHRVHS
                     AVAVTDAGGRTRTRLSTSLVAFKRLSEEEIQAYLASGEWLGKAGGYAIQGRAEALVRM
                     LQGSHSGVMGLPLFETRALLRAAGLPVG"
BASE COUNT          540 a         1158 c         1095 g          513 t
ORIGIN      
        1 agagacgaag cggatcgtcg aggcggaggg ccggcgggcc gtggcgattc ccggcgacgt
       61 cggcgacaag gacttttgcg agcgcgccgt ttcccagacg gtgaaggaac tcggccagct
      121 cgacatcctc gtcaacaacg ccgccgaaca gcatccggac aaggagatca ccgagatcac
      181 cgaggagcag ctgaagcgga cgttccagac caatttctac ggctatttct tcatggtcca
      241 ggcagcgcgg ccgcacctga agaaaggcgc cgcgatcgtc aactgcacgt cggtgaccat
      301 gtacaaggga tcggagaagc tgctcgatta ttcggcgacc aagggcgcca tcaccgcctt
      361 cacccgctcg ctatcgcaga atctgatcga ggatgggatt cgcgtcaacg ccgttgctcc
      421 cgggccgatc tggactccgc tcaacccctt cggcgggcag ccgccggagg agatccccga
      481 tttcggcaag gacgtgccga tgggccgccc cggccagccc aacgaagtgg cgccgagctt
      541 cctcttcctc gcctgcgagg attcgagcta catggcgggc caggtgcttc acccgaatgg
      601 cggcgaggtc gtcaacggct agagatgcgg gggtgccgcc agcgcgattc gtgctatagc
      661 cgcccctccg gacaaggggc gacgatggtt aggccgataa ccggcatcgt gcacgccgcg
      721 cgcgcatgac ccgctacaca agcttccgcg acttctggcc gcattatctg cgcgagcata
      781 agcggccggc gacccgaagg ctccattacg cgggcaccgg cctggtcctc ggcttcgccg
      841 tggcggccgc ggcgagccgg gactggcggc tcctcgccgc cgtcccgctg gccggctacg
      901 gcttcgcctg gttcagccat gccgccgtcg agcgcaaccg gccggcgacc ttcacctatc
      961 ctctctggtc gctcgccgcg gacttcagga tggcctggct ctgggcgacg gggcggctcg
     1021 attccgagct ccgcgaggcg ggggtggaac cggagcgtcg aaagggggaa ggcgatgcgc
     1081 tctgagatga ccgacgccga cgccggcgcg gccatagcag gactgctcct caagctcagg
     1141 gcgcgggacc tggtcggccg ggaggaggag gacgtgcttc gagcctccgt ctcggaactt
     1201 caggagcatc ccgcgggacg aacgatcgtc cgcaccggaa cgaccctcag cgcctccacc
     1261 ctgctcgtcg agggcatcgt ctgccgcttc aaggacctcg ccgacggcca gcggcagatc
     1321 atggagcttc acgtcggcgg cgacttcgtc gaccttcacg gcttcctctt gaagcagctc
     1381 gaccacaatg tcggagcgat gaccccggtc cgggtcgcga gcgttccgca cgacgcgctt
     1441 cgcggaatca ccgagaccca cccgcatctg ggccgaatgc tctggttctc gaccctgctc
     1501 gacgccgcca tccaccgcga gaagatcctg tcgatcggcc ggcgcagcgc gctggcgcga
     1561 atcgcgcata tcttctgcga gctcctcgtc cggctgcgca tcgtcgggct cgcggacgac
     1621 aatggctacg ccttgcctct gacccaggcg gacctcgccg actccaccgg actcacctcg
     1681 gtccacgtca accggatgct caagaagctg cgcgacgaaa atctgctcac cttccgcggc
     1741 ggcgaggtcg cgatcggcga ctgggaacgg ctccagcgcg tggcggagtt cgacccgacc
     1801 tacctccatc tggagcgccg gccgcggtag aacgggggcc ttgccggctc cgtcgtcccg
     1861 gcgcaggccg ggatccatga acacggtctt tcgaaaaggg tccgactcgg ccgcgcctcc
     1921 tgcggcggtt tccgtgttca tggatcccgg ccttcgccgg gatgaccttc atcctacagg
     1981 tcccgaacct ccaagggcac ccgctcaacc atccgctgag ccagcatcga gcggtggcag
     2041 tcgggcgctc cgcgctcata gcagagcagg gccgagggtc gggcggccgc ttcggccagc
     2101 gcctgctcca tcgccagccc cgcctccgga gtttcgagct gtaccgcgta gatggcgcgc
     2161 atctcggcga tccgcccggc tctcgccgcc tggcgcccgt ccgccggggt accgagcgag
     2221 cgcaggtgca ggtagtctat tccgcgctcc ctcaggcccc cggcaagggc ggtcttcgag
     2281 aagcccggcc gccgcgagga tgcgacggcg cgaacgtcga ggatcagctc gacgcccgcg
     2341 gcctgaagct cgtccaggaa ctccgcctgg gtcttgccct catagccgat cgtgaacagc
     2401 gtgatcatcg ccatcgccta gcaaagccgc gtggccaaag cttccctttc cgccgatcag
     2461 ccgttatggc ggcgcggttt tgcgagccgg ttcaagaggg taaatggcca aggaagagct
     2521 actcgagatg cgcgggcagg tggtcgagct gctgcccaac gccatgttcc gcgtgaaact
     2581 cgagaacaat cacgagatcc tcggccacac cgccggcaag atgcgcaaga accgcatccg
     2641 ggtcctcgtc ggcgacgagg tgctcgtcga gctcaccccc tacgacctga ccaagggtcg
     2701 gatcacctac cgcttcaagt gacggggccg gtccccgtgc gactgatcct cgcctcggcc
     2761 agtccccgcc gcctcgagct gctggcgcgc ctgggcgtga ttccggacgc ggtgatcccg
     2821 gccgagatcg acgagactcc gcgcaaggcg gagattcccg atcgctatgc ctgccggatg
     2881 gcgacggaga aggcggcggc ctgggccgaa ccgggcagcc tcatcctcgc cgcggacacg
     2941 gtggtcgcag cgggccggcg gatcctcccc aagacggaga gcgaggacga agcgcgagcc
     3001 gccctggcgc tcctctccgg gcgccgccac cgggtccatt cggccgtcgc cgtgaccgac
     3061 gccgggggtc gcacccgaac gcgcctctcg accagcctgg tcgccttcaa gcgcctctcc
     3121 gaagaggaga tccaggccta tctcgccagc ggcgagtggc tcggcaaggc ggggggctat
     3181 gcgatccagg gccgggccga ggcgctggtc cggatgctcc agggcagcca ttccggcgtg
     3241 atgggccttc ccctgttcga gacgcgggcg ctgctgcggg cagcgggctt gccggtcggc
     3301 tgatgg
//
LOCUS       DASWNU010000102         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_263094, whole genome shotgun sequence.
ACCESSION   DASWNU010000102 DASWNU010000000
VERSION     DASWNU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263094"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VGB57_02025"
     CDS             <1..1081
                     /locus_tag="VGB57_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY0130153.1"
                     /translation="DLYRRLGAEPGNIFISPASVSMALGMVHAGAEGETAAEMERALR
                     YPPGAVHEGMGALLESLPIALEGRRLSLANALWVQQSFALRPSYLEQVRRHYGGGARP
                     VDFVGAPVEAIAAVNRWAEEQTAGRIKTILQRENINDRTRLVLTNAVYFKADWLLPFQ
                     ASQTRPRPFRLAGGGSVSVPMMRQRANFRLLETPAFEAVEMPYKGEELSMLLFLPRES
                     SGLKQFEQSLDGQALRGWTEQLMSSERRDLELVAPRIELDTRASLVPQLQALGMRRAF
                     TQLAQLGGMSEARLYLSDVIHQTYLRIDEKGTEAAAVTAGLAEIVSMPRQFHADRPFF
                     FVIRDNRSGTLLFLGRIERPSLPKA"
     gene            1126..>1693
                     /locus_tag="VGB57_02030"
     CDS             1126..>1693
                     /locus_tag="VGB57_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130154.1"
                     /translation="MRPILLPLLALVACQPKAEPAPPPLPEPPAPAVAKALPRHWTGV
                     FTMVEASGVRAGAGQFQLRLGLEAPNHFRAIRGCYTQQGFLEPRDGVWTVRQRGGIQV
                     DEQCLARQVGLGRGSPEGLFQHREIVLSPPGQLWIAEGNARWTYLPNPSAPPLPAPPP
                     PPPAPGPGPGPPGGGINESSLDAAEALYR"
BASE COUNT          250 a          624 c          560 g          259 t
ORIGIN      
        1 cgacctctac cgccggctcg gggcggagcc cggcaatatc ttcatctccc cggcgagcgt
       61 ctccatggcg ctgggcatgg tccatgccgg cgccgagggc gaaaccgccg ccgagatgga
      121 gcgggcgctc cgctatccgc cgggggccgt gcacgagggc atgggcgcct tgctcgaaag
      181 ccttccgatc gccctggaag gccgccggct cagcctcgcc aacgccttgt gggtccagca
      241 gagctttgcg ctccggcctt cctatctgga gcaggtccgg cgccattatg gcggcggcgc
      301 ccggccggtc gatttcgtcg gcgcaccggt cgaggcgatc gcggccgtca accgctgggc
      361 cgaggagcag accgccggcc ggatcaagac catcctgcag cgcgagaaca tcaacgaccg
      421 cactcgcctg gtcctgacca acgccgtcta tttcaaggcc gactggctcc tgcctttcca
      481 ggcctcccag acccgccccc ggccgttccg gctggccggc ggcggatcgg tttccgtccc
      541 gatgatgcgc cagcgcgcca acttcaggct cctcgaaacc ccggccttcg aagccgtgga
      601 gatgccgtac aagggcgaag agctgtcgat gctgctcttc cttccaaggg agagcagcgg
      661 cctcaagcag ttcgagcagt ccctggatgg gcaggcgctg cgcggatgga cggagcagct
      721 gatgtcgagc gagcgccgcg acctcgagct ggtcgcccca aggatcgagc tcgacaccag
      781 ggcctcgctc gtgccccagc tccaggcgct cggaatgagg cgcgccttca cccagcttgc
      841 ccagctcggc ggcatgtccg aggcccgcct ctatttgagc gacgtcatcc accagaccta
      901 tttgcggatc gacgagaaag ggacggaggc ggccgcggtc accgcgggcc tggccgagat
      961 cgtcagcatg ccgaggcaat tccacgccga tcgccccttc ttcttcgtca tccgcgacaa
     1021 ccggtccgga actctcctct ttctcgggcg gatcgagcgg ccgtcgcttc caaaggcctg
     1081 agcggcgcgc ttggagcgtc ggcggcccgg aggcgtttcc ccgccatgag accgatcctc
     1141 ctgccgctgc tggcgctcgt cgcctgccag ccgaaggccg agccggcacc gcccccgctt
     1201 cccgagcctc cggctccggc ggtcgcgaag gccctgccga ggcactggac cggagttttc
     1261 acgatggtgg aggccagcgg cgtccgcgcc ggcgccggac agttccagct gaggctgggg
     1321 ctcgaggcgc ccaatcattt ccgggccata aggggttgct acactcaaca gggcttcctc
     1381 gagccccgcg acggcgtgtg gaccgttcgc cagcgcggcg gaattcaggt cgacgagcaa
     1441 tgcctcgctc ggcaggtcgg cctcgggcgg ggatcgcccg aaggtctctt ccagcaccgc
     1501 gagatcgtcc tgtcgccgcc cgggcagctc tggatcgccg aggggaatgc gcgctggacc
     1561 tacctcccca atccctcggc gccgccactg cccgcgccac cgccgccacc gcccgcgccg
     1621 ggcccgggcc cgggcccgcc gggaggcggg ataaacgagt ccagtctgga cgccgcggag
     1681 gcactctatc gcc
//
LOCUS       DASWNU010000103         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_340989, whole genome shotgun sequence.
ACCESSION   DASWNU010000103 DASWNU010000000
VERSION     DASWNU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340989"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGB57_02035"
     CDS             <1..649
                     /locus_tag="VGB57_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY0130155.1"
                     /translation="EGERSHQYRILRATKNRFGGTDEIGVFAMAEGGLEEVGNPSALF
                     LTHRGEAVSGAVVFPALEGTRPVLVEIQALTVRLASGATPRRAVVGWDSGRLAMILAV
                     LEARCGLSFSSSEVYLNVAGGYRLSDPAADLAVAAALVSALAERPVPPEAVAFGEVAL
                     SGELRPVAHGPLRLKEAAKLGFERALVPASVQGEGGMRLSGFRTLKVLVDHVLGR"
     gene            829..1410
                     /locus_tag="VGB57_02040"
     CDS             829..1410
                     /locus_tag="VGB57_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY0130156.1"
                     /translation="MTALDVIVLLLVGWNLVRGAMKGFVGEVLSLAAVIVAIVFLKLL
                     HTPVTNALEGAVGTRSGAAVLAFALVFGLVFLAGKLVARRVGGGVKRSVIGPIDRVLG
                     AGFGVVKGILLATLLYLGANLVYDTIWGAQSERPAWMANSRTYPLLYASGRAIVDFVE
                     WRRGAPAEPRYRQPPARESEPPPAKDSETANAS"
     gene            1407..>2233
                     /gene="cysS"
                     /locus_tag="VGB57_02045"
     CDS             1407..>2233
                     /gene="cysS"
                     /locus_tag="VGB57_02045"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0130157.1"
                     /translation="MIRLHDTKAREKRDFEPGDPERVTVYVCGPTVYNRAHIGNARPA
                     VVFDALVGLLRHTYGADKVVYARNVTDVDDKIIDAARAEGVDPAVVTRRYEDFYVADM
                     GALGVRPPDLAPKATETVAEMVAMIARLVETGHAYEADGHVLFHVPSDPGYGALGRRD
                     REAMIAGARVEVASYKKDPSDFVLWKPSPEGVIGWDSPWGRGRPGWHIECSAMIEKHL
                     GQTIDIHGGGLDLIVPHHENELAQSRCTHRGEPLARYWLHNGFLSMAGSEKMSKSLG"
BASE COUNT          327 a          787 c          761 g          358 t
ORIGIN      
        1 cgaaggcgag cgaagccatc agtacaggat ccttcgagcg accaagaacc gcttcggcgg
       61 aaccgacgag atcggggtct tcgccatggc cgaaggcggg ctcgaggagg tcggcaaccc
      121 gagcgcgctc ttcctcaccc atcgcggcga ggcggtttcc ggagcggtcg tcttccccgc
      181 gctggaaggg actcggccgg tcctggtcga gatccaggcg ctcaccgtcc gactcgccag
      241 cggcgccact ccgcgccggg cggtggtcgg ctgggattcg ggacggctgg ccatgatcct
      301 ggcggtgctg gaggcgcgct gcggcctcag cttctcgagc tcggaagtct atctcaacgt
      361 cgccggcggc taccggctga gcgacccggc cgccgacctc gccgtggccg cggcattggt
      421 gtcggctctg gcggagcggc ccgtgccgcc cgaggcggtg gctttcgggg aggtcgcgct
      481 ctccggcgag ctccgcccgg tggcgcacgg gccgcttcgg ctcaaggaag cggccaagct
      541 ggggttcgag cgcgcgctgg tgccggcctc ggtgcagggc gagggcggga tgaggctgtc
      601 gggcttcagg acgctgaagg ttctggtcga tcatgtcctg ggacggtgat ttgcctcccc
      661 atcgcaagat ggggaggggg accatgcgaa gcatggtgga ggggtaattg acgaggcctg
      721 ctccagttac ccctccacca cccttcgggt ggtccccctc cccacgctcc gcgtggggag
      781 gcaggtccgc gcttccctcg ctcccaatcc tccgttagca gtcgcagcat gaccgccctc
      841 gacgtcatcg ttctgcttct cgtcggctgg aacctcgttc gcggggcgat gaagggtttc
      901 gtcggcgagg ttctgagcct cgccgccgtg atcgtcgcca tcgtgttcct gaagctgctt
      961 cacacgcccg tcaccaacgc gctcgaaggc gcggtcggca cccgctcggg cgcggccgtc
     1021 ctcgctttcg ccttggtctt cggcctcgtc ttcctggccg gaaagctggt cgcccgccgc
     1081 gtcggcggcg gggtcaagcg ctcggtgatc ggcccgatcg accgggtgct cggcgcgggc
     1141 ttcggagtgg tgaagggcat cctcctcgcg accttgctct atctgggcgc gaacctcgtc
     1201 tacgacacga tctggggcgc ccagtcggag cggcccgcct ggatggccaa ttcgcgcact
     1261 tatcctttgc tctacgcgag cggccgcgcc atcgtcgatt tcgtggaatg gcggcgtggg
     1321 gcgcccgccg agccgcgcta ccgccagccc cccgccaggg agagcgagcc cccgcccgcc
     1381 aaggacagcg agaccgcgaa cgcatcatga tccgactcca cgacaccaag gcgcgggaga
     1441 agcgcgactt cgagccgggc gatccggagc gggtgacggt ctacgtctgc ggtcccaccg
     1501 tctacaaccg ggcccatatc ggcaacgccc ggccggccgt ggtcttcgac gccctcgtcg
     1561 gactgcttcg ccacacctac ggcgccgaca aggtcgtcta tgcccgcaac gtcaccgacg
     1621 tcgacgacaa gatcatcgac gccgcccgcg ccgagggggt cgacccggcc gttgtcaccc
     1681 gccgctacga ggatttctac gtcgccgaca tgggcgcgct cggagtccgc ccgcccgacc
     1741 tcgccccgaa ggcgaccgag accgtggccg agatggtggc gatgatcgcc cgcctggtcg
     1801 agaccggtca tgcctacgaa gcggacggcc acgtcctctt ccacgttccg tcggaccccg
     1861 gctacggcgc gctcggccgc cgcgaccgcg aggcgatgat cgccggcgcc cgggtggaag
     1921 tcgcctccta caaaaaggac ccgtccgatt tcgtgctgtg gaagccgtcg ccggaaggcg
     1981 tgatcggctg ggacagcccc tggggccgcg gccggccggg ctggcacatc gaatgctcgg
     2041 cgatgatcga gaagcacctc gggcaaacca tcgacatcca cggcggtggg ctggacctga
     2101 tcgtcccgca ccacgaaaac gaactcgccc aaagccgctg cacccaccgc ggcgagccgc
     2161 tcgcccgcta ctggctgcac aacggcttcc tttcgatggc cggttccgag aagatgtcga
     2221 agagcctcgg caa
//
LOCUS       DASWNU010000104         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_314893, whole genome shotgun sequence.
ACCESSION   DASWNU010000104 DASWNU010000000
VERSION     DASWNU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314893"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VGB57_02050"
     CDS             complement(<1..446)
                     /locus_tag="VGB57_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130158.1"
                     /translation="MKPLRAKLLFLAGGAALAFALPAMGQREKGPESLLPPGFGEPPP
                     PAPKAPPSDAPATPSPASPAPPAPSPARPRSAAPLGIEDAAAEDLEALAQQALPPPIE
                     IPEGSRRPVNPVGPLRPGNWGLGLDAFGNANGRFLSGLMGRLNAPLP"
     gene            complement(427..594)
                     /locus_tag="VGB57_02055"
     CDS             complement(427..594)
                     /locus_tag="VGB57_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130159.1"
                     /translation="MRISPPPRRRRSIVPKLLIALLLLFLGWLIWLGFQSTEVPTQRI
                     EQDVTNEALAG"
     gene            644..1180
                     /locus_tag="VGB57_02060"
     CDS             644..1180
                     /locus_tag="VGB57_02060"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0130160.1"
                     /translation="MRHALLPSERSIVLVGLMGVGKSTVGRRLARRLGLPFVDSDEEI
                     ERAADHEIKEIFDRFGEASFRDGERRVIRRLIEGERKVIATGGGAFVDAETRALILDR
                     CIAIWLEADVEILARRVARRAHRPLLAGKDPLPFLSGLAEVRNPVYAEAHLRVSSGTG
                     PHEKTVERIVEALAEQPG"
     gene            1177..2253
                     /gene="aroB"
                     /locus_tag="VGB57_02065"
     CDS             1177..2253
                     /gene="aroB"
                     /locus_tag="VGB57_02065"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY0130161.1"
                     /translation="MKRIEVALAERSYTIVIAGGLIERAGELLAPYARSGRIVAVSDE
                     IVWAAQGPRLLASGLEIVPVLVPPGEASKSWTQLQALTERLLDLGVERGDSILAFGGG
                     MVGDLAGFAASILKRGCPYVQVPTTLLAQVDSSVGGKTAINAAAGKNLIGAFHQPAAV
                     LIDPSTLDTLPERELRAGYAEVVKYGLIGDPAFFAWCEANAAALLAGDEAARLNAIET
                     CVRAKAAIVAEDERETNGRRALLNFGHTFGHALEAETGFAMLHGEAVAVGMTLAFRFS
                     AERGLCGTEEAERVTVHLGAAGLPVRLEGVSAERLVQHMARDKKGSGGRVPFVLTRGI
                     GEAFVDRSVELGQVEAFLERELGG"
     gene            complement(2381..>3205)
                     /locus_tag="VGB57_02070"
     CDS             complement(2381..>3205)
                     /locus_tag="VGB57_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0130162.1"
                     /translation="LFGLAKAFGMKSRRALKLGLLLSQGGEFGFVLFAAAQGALIIEP
                     AAASLFGAIVTVSMATTPFLMMLNDWLDRRSSRRAGDDLDGPELSGPTQAIVVGYGRF
                     GQTVAQMLMAKGITVTLIDSKPSQIETSGAFGSKVYYGDGTRLDLLRLAGAGDAKAIL
                     FCLDDRAPTARRLEPILEAFPQAAVFVRAFDRLHLMELAPLDIKCTIREIFESAVMMG
                     REALALFCIDADEVDRVEQEYRERDRQRLDSQTASGDIHALKENMFSPDNPLGKAP"
BASE COUNT          500 a          996 c         1237 g          472 t
ORIGIN      
        1 ggcagcggag cgttgagccg gcccatgagg ccgctgagga agcggccgtt ggcattgccg
       61 aaggcgtcga ggccgagccc ccaattgccg ggacgaagcg gcccgaccgg gttgacggga
      121 cggcgcgagc cttccggtat ctcgatcggc ggcggcagcg cctgctgggc gagcgcctcc
      181 agatcctcgg cggcggcatc ctcgatccca agcggcgcgg cggagcgcgg ccgggccggc
      241 gagggcgcgg gaggcgcggg cgaggcgggc gacggcgttg ccggagcgtc ggagggcggg
      301 gccttgggcg caggcggggg cggctcgccg aagccgggtg gcagcagcga ttccgggccc
      361 ttttcgcgct ggcccatcgc cggaagggcg aaggccaagg ccgcgccgcc ggcgagaaag
      421 aggagcttag cccgcaaggg cttcattggt gacgtcctgc tcgatccgct gggtcggaac
      481 ctcggtcgac tggaagccga gccagatcag ccagcccagg aaaaggagaa ggagggcgat
      541 aagcagcttg ggcacgatgg atctgcgccg gcgcggcggg ggtgagatac gcatggccgg
      601 ccttttgccc cacgagccgc ccccctgtat agcccctgcc gcaatgcgac acgcgcttct
      661 cccgtccgaa cgctccatcg tcctcgtcgg gctgatgggc gtgggtaaat cgacggtcgg
      721 ccggcggctg gcgcggcggc tcggcctccc cttcgtcgat tcggacgagg agatcgagcg
      781 cgccgccgac catgagataa aggagatatt cgaccgcttc ggcgaagcga gcttccgcga
      841 cggcgagcgg cgcgtgatcc ggcgactgat cgagggcgag cgcaaggtga tcgcgaccgg
      901 cggcggcgcc ttcgtggacg cggagacgag ggcgctgatc ctcgatcgct gcatcgcgat
      961 ctggctcgag gcggacgtgg agatcctggc gcggcgggtg gctcggcggg cccaccggcc
     1021 gctcctcgcc ggcaaggacc ccctgccctt cctgagcggg ctcgccgagg tccgcaaccc
     1081 ggtctatgcc gaggcccatc tgcgggtgag cagcgggacg gggccccacg aaaagacggt
     1141 ggagcggatc gtcgaggcgc tcgcggagca acctggatga agcggataga ggtcgcgctg
     1201 gccgagcgca gctacacgat cgtcatcgcc ggcggactga tcgagagagc gggggagctg
     1261 ctcgcgcctt atgcccggag cgggcgaatc gtggcggtgt cggacgagat cgtctgggcg
     1321 gcgcagggcc cgcggctcct ggcctcgggg ctcgaaatcg tgccggtgct ggttccgccg
     1381 ggcgaggcga gcaagagctg gacacagctt caggcgctga ccgagcggct gctcgatctc
     1441 ggggtcgagc ggggcgattc gatcctcgcc ttcggcggcg gaatggtcgg cgacctcgcc
     1501 ggcttcgccg cgtcgatcct gaagcggggc tgtccctatg tgcaggtgcc gacgaccttg
     1561 ctggcgcagg tggattcgtc ggtcggcggc aagaccgcga tcaacgccgc ggcgggcaag
     1621 aatctgatcg gcgccttcca ccagccggcg gcggttctga tcgatccctc gactctcgac
     1681 acgcttcccg aacgggagct gcgcgcgggc tatgccgagg tggtgaaata cgggctgatc
     1741 ggcgatcccg ccttcttcgc ctggtgcgag gcgaacgcgg cggccctgct ggcgggcgac
     1801 gaggcggcgc ggctcaatgc gatcgagacc tgcgttcgcg ccaaggcggc gatcgtcgcc
     1861 gaggacgaga gggagacgaa tggacggcgg gcgctgctca atttcggcca tacgttcggg
     1921 catgcgctgg aggcggagac cggcttcgcg atgcttcacg gggaggcggt ggcggtcgga
     1981 atgaccctgg ccttccgctt ctcggcggag cgagggctgt gcgggaccga agaggcggag
     2041 cgggtcacgg tgcatcttgg ggcggcgggg ctgcccgttc ggctggaggg ggtgagcgcg
     2101 gagcggctcg tccagcacat ggcgcgcgac aagaagggaa gcggcgggcg ggtgccgttc
     2161 gtgctgacgc ggggaatcgg cgaggctttc gtcgaccggt cggtcgagct cggccaagtg
     2221 gaggcgttcc tagagcggga gttgggaggc taaattcctc ccccgcattt gcgggggagg
     2281 gggaccatgc gaagcatggt ggaggggttc tgcccgactc cgaagaaccc ctccaccgcg
     2341 ctacgcgcgg tccccctccc cgcgaaatcg cggggaggat ttatggagcc ttgcccagcg
     2401 gattgtccgg ggagaacata ttctccttga gcgcatggat gtcgcccgac gcggtctggc
     2461 tgtcgaggcg ctggcggtcg cgctcgcgat attcctgctc gacccggtcg acctcgtcgg
     2521 cgtcgatgca gaacaaggcg agcgcctcgc ggcccatcat caccgccgat tcgaatatct
     2581 cgcggatcgt gcatttgatg tcgagcggcg ccagctccat cagatggagg cggtcgaagg
     2641 cgcggacgaa cacggcggcc tgagggaagg cctcgaggat cggctccagg cgccgggccg
     2701 tgggggctcg gtcgtcgagg cagaagagga tcgccttggc gtcgccggct ccggcgaggc
     2761 gaagaaggtc gaggcgcgtg ccgtcgccat aatagacctt cgaaccgaaa gcgccgctgg
     2821 tctcgatctg gctgggtttc gagtcgatca gggtaacggt gatgcccttc gccatcagca
     2881 tctgggcgac ggtctgcccg aagcggccgt agccgacgac gatcgcctgg gtcggccctg
     2941 aaagctccgg gccgtcgaga tcgtcacccg cgcggcggct ggagcggcgg tcgagccagt
     3001 cgttgagcat catcaggaac ggagtcgtcg ccatcgagac ggtgacgatg gcgccgaaca
     3061 ggctcgccgc cgccggctcg atgatcaagg cgccctgggc ggcggcgaac aggacgaagc
     3121 cgaactcgcc gccctggctg agcagcaagc ctagcttgag cgcccgccgc gacttcatcc
     3181 cgaaggcctt ggcgaggccg aagag
//
LOCUS       DASWNU010000105         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_133330, whole genome shotgun sequence.
ACCESSION   DASWNU010000105 DASWNU010000000
VERSION     DASWNU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133330"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGB57_02075"
     CDS             <1..572
                     /locus_tag="VGB57_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0130163.1"
                     /translation="SAARRHMLEAAGVPFETVEAELDEAAAKGGLEAARFDARGIAEE
                     LAQLKALSVPPAANALVLGSDQTLELEDGSLLGKPESRSEALEQLSRLSGRTHLLHSS
                     AVVAEHGEAVWWHSETVELTMRRLSPAFLDDYLDREFEQIRWSVGGYRIEGLGAQLFD
                     RIEGSHFAILGMPLLPLLGYLRERGLLKS"
     gene            572..1405
                     /locus_tag="VGB57_02080"
     CDS             572..1405
                     /locus_tag="VGB57_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0130164.1"
                     /translation="MGIPYAEVIGDPIAHSKSPIIHGFWLRKVGVEGDYRACRVTSDG
                     LAGYFSRRSKDPDWRGCNITMPHKYAVLDHVHFHQDPTFPVEPANTAFRRDGSERIEG
                     CNFDKNGFMEPLMPMHGGRGLRPRGPAVIVGAGGAAVAAAFMMPVMGYGPIWIVARDP
                     AKAAKMADDFSGLGAFAASFRDPLPEARLLVNASPLGMRGFPPFPFSLDRMAADGIVY
                     DMVYSPAETALLREARRRELQTVDGLAMLIGQAALAFESFFRVRPPRAFDHELREALL
                     R"
     gene            1402..1995
                     /gene="coaE"
                     /locus_tag="VGB57_02085"
     CDS             1402..1995
                     /gene="coaE"
                     /locus_tag="VGB57_02085"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369101.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0130165.1"
                     /translation="MIILGLTGSIGMGKSTVARMFFEEGVPVFDADAAVHRLQGPDGA
                     LVERIEAEFPNTTGPEGVDRGALAERVLGEPEALRRLEALVHPAVAREREAFLAANGS
                     APVVVLDIPLLFEAGGSGQVDRIAVVSAPAEVQRDRVLARPGMTEEKFERILARQLPD
                     EEKRARADFVIPTGGSLEDTRRAVRRILACLVPSADS"
     gene            2003..2716
                     /gene="dnaQ"
                     /locus_tag="VGB57_02090"
     CDS             2003..2716
                     /gene="dnaQ"
                     /locus_tag="VGB57_02090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY0130166.1"
                     /translation="MREIVFDTETTGLSPLTGDRVVEIGCVELWNRVETGRTFHAYFN
                     PERPMPAGAEAVHGLSDAFLADKPRFRDLARDLLEFLGECPLVAHNASFDFGFLNHEL
                     NNCGHSLICLSRMVDTLQIARSKHPGAKHSLDALCTRFGVDRSLRVKHGALIDAQLLA
                     QCYVELTGGRQIGLTLTDIAAERIEEAAEALAAGSDTLVIEVRPPRAHAPTEEELQAH
                     ATFLATLIDPIWHRLDGRG"
     gene            2768..3145
                     /gene="raiA"
                     /locus_tag="VGB57_02095"
     CDS             2768..3145
                     /gene="raiA"
                     /locus_tag="VGB57_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY0130167.1"
                     /translation="MDIRVSGHQLDTGEALRERVSTRLAAAADKYSSRLVSAQVTFGK
                     GPHDHGFTCEIVAYAPQGVVLKATDRAAAAPAAFDAALEKVEKQLRRHSRRLKDHHPQ
                     AAAELAEGDAFAYRRGESQPQAE"
     gene            3219..3686
                     /locus_tag="VGB57_02100"
     CDS             3219..3686
                     /locus_tag="VGB57_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0130168.1"
                     /translation="MNDITDILTVDRVDGGMNVPNKKGLFQLLGAAAGRRTGLDSKTI
                     VAALADREKVGSTGFGGGVAIPHGRIEGLKSVFGYFARLTAPIDFQSVDNLPVDLVFL
                     ILSPPDAGADHLKALASVSRVLRDRNTVAKLRGARSRDALFALLSGVESRDAA"
     gene            3676..>4091
                     /locus_tag="VGB57_02105"
     CDS             3676..>4091
                     /locus_tag="VGB57_02105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0130169.1"
                     /translation="MPPEPDAGAAAHFRALESLYRHAPVNGLFESRLEIVEKGFARIR
                     FEVGERLFHAAGAAHGTLYFKMMDDAAFYACNSLVTDRFLLTTAFNLLFTRPLRAGPV
                     IAEGRWASGRRRVLVGEARLIDSEGELAASGTGTFMR"
BASE COUNT          634 a         1429 c         1356 g          672 t
ORIGIN      
        1 agagcgccgc ccgccggcac atgctcgaag cggctggcgt gccgttcgag acggtggagg
       61 cggagctgga cgaagcggcg gccaagggcg ggctcgaagc cgccaggttc gacgcgcgcg
      121 ggatcgcgga ggagctcgcc cagctcaagg cgctctccgt cccgcccgcg gcgaatgcgc
      181 tggtgctcgg ctccgaccag acgctcgagc tcgaggacgg cagcctgctc ggcaagccgg
      241 aatcgcgctc cgaggcgctg gagcagctct cccgcctcag cggccggact cacctgctcc
      301 attcgtccgc ggtcgtcgcg gagcatggcg aggcggtgtg gtggcacagc gagaccgtcg
      361 agctgaccat gcgacgcctc agccccgcct ttctcgatga ctatctcgac cgcgaatttg
      421 agcagatccg ctggagcgtc ggcggctacc ggatcgaggg cctgggcgcg caactcttcg
      481 accgaatcga gggcagccat ttcgccatcc tgggaatgcc gctcctcccc ttgctcggct
      541 atctgcgcga gcgcgggctc ctgaagagct gatgggaatc ccctatgccg aggtgatcgg
      601 cgacccgatc gcccattcga agtcgccgat catccatgga ttctggcttc ggaaggtcgg
      661 tgtggaaggg gattatcggg cgtgccgggt cacgtccgac ggattggccg gctatttctc
      721 tcgacgttcg aaggatccgg actggcgagg gtgcaacatc accatgccgc acaaatatgc
      781 ggtgctcgat catgtccatt tccatcaaga ccccaccttc ccggtagagc cggccaacac
      841 cgctttccgc cgcgacggat cggagcggat cgaaggctgc aacttcgaca agaacgggtt
      901 catggagccg ctgatgccca tgcacggcgg ccggggttta cggccgaggg gcccagcggt
      961 gatcgtcggc gccggcggag cggccgtagc cgccgcgttc atgatgccgg tcatgggcta
     1021 cgggccgatc tggatcgtcg cgcgcgatcc agccaaggcg gcgaagatgg cggacgattt
     1081 ctcggggctg ggcgcattcg cggcgtcgtt ccgcgatcca ctgccggagg cgcggctgct
     1141 cgtgaacgct tcaccgctgg gcatgcgcgg ctttccgcct tttccttttt cgctcgaccg
     1201 aatggccgcg gacgggatcg tctatgacat ggtctattcg ccggcggaga ccgccctgct
     1261 tcgcgaggcg cggcggcggg agctgcaaac cgtcgacggc ctggcgatgc tgatcggcca
     1321 ggccgcgctc gccttcgaat ccttcttccg ggtgcggccg ccacgagcct tcgaccacga
     1381 gcttcgggaa gctctcctgc gatgatcatc ctcggcctca ccggatcgat cggaatgggc
     1441 aagtccaccg tcgccaggat gttctttgag gaaggcgtgc cggtcttcga cgccgatgcg
     1501 gcggtgcacc ggctgcaggg gccggatggg gcgctggtcg agcggatcga ggccgaattc
     1561 ccaaacacca ccggccccga aggcgtggat cgcggcgcgc tggcggagcg ggtactcggc
     1621 gagcccgagg cgcttcggcg gctcgaggcg ctggtccatc cggcggtggc gcgcgagcgg
     1681 gaggctttcc tcgccgccaa cggatccgcc ccggtggtcg tgctcgatat cccgctcctg
     1741 ttcgaagccg gcggatccgg ccaggtcgac aggatcgccg tcgtctcggc gccggccgag
     1801 gtccagcgcg atcgagtcct cgcccgcccg ggcatgaccg aggagaaatt cgagcggatc
     1861 ctggcccggc agctcccgga cgaggagaag cgcgcccgag ccgatttcgt catccccacc
     1921 ggcggttcgc tggaggacac gcgacgagcg gttcggcgca tcctcgcttg cctcgtcccg
     1981 tccgccgatt catagtctgg tcatgcggga aatcgtcttc gacacggaaa ccacgggcct
     2041 cagcccgctc accggcgacc gggtcgtcga gatcggctgc gtcgagctgt ggaaccgggt
     2101 cgagaccggc cgcaccttcc atgcctattt caacccggag cggccgatgc cggccggcgc
     2161 cgaggcggtg cacggcctca gcgacgcctt cctcgccgac aagccgcgct tccgcgacct
     2221 ggcgcgcgac ctgctagaat tcctcggcga gtgtccgctg gtcgcgcaca atgccagctt
     2281 cgatttcggc ttcctcaacc acgagctcaa caattgcggg cattcgctca tctgcctgtc
     2341 gcggatggtc gacactcttc agatcgcccg ctccaagcat cccggggcca agcacagcct
     2401 ggatgcgctc tgcacccggt tcggggtcga tcgaagcctt cgggtgaagc atggcgccct
     2461 gatcgatgcc cagctcctcg cccaatgcta tgtcgagctg accggcggcc gccagatcgg
     2521 cctcaccctc accgacatcg ccgccgagcg gatcgaagag gccgcggagg cccttgccgc
     2581 cggctccgat accctcgtca tcgaagttcg gccgccccgg gcccatgccc cgaccgagga
     2641 agagcttcag gcgcatgcca ccttccttgc gacgctcatc gaccccatat ggcaccggct
     2701 ggacgggcgc ggttgacgtt gagccgcggc ggcgcctaat ccccgcggca ctttgctgga
     2761 gaaagccatg gatatccgcg tctccggtca ccagctcgac accggcgaag ccttgcgcga
     2821 acgggtaagc acccggctcg ccgccgccgc cgacaaatat tcgtcgcggc tggtctcggc
     2881 ccaggtgacg ttcggcaagg ggccgcacga tcatggcttc acctgcgaga tcgtcgctta
     2941 tgcgccacag ggggtggtcc tgaaggcgac cgatcgcgcc gccgcagcgc cggcggcgtt
     3001 cgacgcggcc ctcgagaagg tggagaagca gctccgccgc cactcccgcc gtctcaagga
     3061 ccatcatccg caggccgcag cggaactggc ggaaggcgac gccttcgcct accgacgagg
     3121 cgagtcccag ccgcaggccg aataagctgt ccggcgggga tccccgcttt ccttcctttt
     3181 tcaaagagcg gccggggggc gaccgcttcg gtgaacagat gaacgacata accgacatcc
     3241 tcacggtcga ccgagtcgac ggcgggatga atgttccgaa caagaagggc ctgttccagc
     3301 tgctcggcgc cgccgcgggg cggcgaaccg ggctcgattc caagaccatc gtcgccgctc
     3361 tggccgatcg ggagaaagtg ggctccaccg gcttcggcgg aggcgtggcc atcccgcacg
     3421 gccggatcga gggcctgaaa agcgtcttcg gctatttcgc ccgtctgacg gcgccgatcg
     3481 acttccagtc ggtggacaat ctgccggtcg acctcgtctt cctgatcctc tcgccgcccg
     3541 acgccggcgc cgaccatctc aaggcgctgg cctcggtaag ccgggtgctt cgcgaccgca
     3601 acacggtcgc gaagctgcgc ggagcgcgtt cccgcgacgc cctgttcgcc ttgctctccg
     3661 gcgtcgaaag ccgagatgcc gcctgagccc gacgccggcg cggccgccca cttccgggcg
     3721 ctcgagtcgc tctaccgcca tgccccggtc aacggcctgt tcgaatcgcg gctcgagatc
     3781 gtcgagaaag gcttcgcccg gatccgcttc gaggtgggcg agcgtctctt ccatgccgcc
     3841 ggcgccgccc acggcacgct ctacttcaag atgatggacg acgccgcctt ctacgcctgc
     3901 aacagcctcg tcaccgaccg attcctgctc accaccgcct tcaacctgct cttcacccgc
     3961 ccgctgcgcg ccgggccggt gatcgccgag ggccgctggg cgagcggccg gcgccgcgtg
     4021 ctggtcggcg aggcgcgact catcgattcg gaaggggagc tcgcggcgag cggcaccggc
     4081 accttcatgc g
//
LOCUS       DASWNU010000106         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_29833, whole genome shotgun sequence.
ACCESSION   DASWNU010000106 DASWNU010000000
VERSION     DASWNU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29833"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..544)
                     /locus_tag="VGB57_02110"
     CDS             complement(131..544)
                     /locus_tag="VGB57_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0130170.1"
                     /translation="MTRTILLYAAVLALAAAALQWLEYRYAAHAFSVEIYIALLAAGF
                     TALGVWAGLRLTPRPAPARFERNDAAIRSLGLTARECELLALLASGQSNKELARALGI
                     SPNTVKTHLARLYEKLEVQKRVQAIEKARWLALIP"
     gene            697..2502
                     /locus_tag="VGB57_02115"
     CDS             697..2502
                     /locus_tag="VGB57_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0130171.1"
                     /translation="MAAPVLAYENLGLIQGSGWLFRGLDLFVGARDRLALIGRNGAGK
                     TTLLKCLAGIIDTDEGRRTIQPGTRVVLLEQDPAVDRFATLREFALHGPEAPAPHEVD
                     AIADQLGIDLDRSAATASGGERRRAAIARALAQDPDVLLLDEPTNHLDLGAIEWLEDW
                     LSRYTGAFIAISHDRTFLKRLTRSSLWLDRGALRRAEIGFGGFDAWTEAVYAEEARAA
                     EKLDARLKLELHWLQRGVTARRKRNQGRLAKLGEMRAARAAMLGPAGAARLGLANDDV
                     KTKMVIDAQKVGKSFGAGESRRAIVRDFTLRIQRGDRIGVVGANGTGKTTLLKLLTGE
                     LEPDEGKIARAKTLQGIMIDQLRRLLDPAKSVRDILADGGDWVEVRDERKHVQGYLKE
                     FLFEPTMVDARIGTLSGGERSRLLLAREFARRSNLLVLDEPTNDLDLETLDLLQEVIA
                     DYDGTVLIVSHDRDFLDNTVTITLGLDGSGKVDIVAGGYEDWQRRRQDGFPAKAGTQS
                     GRKNWAPASAGERKQTKLTYKDQRDYDLLPDRIQAIEAQIARDEEALHDPDLYTRDPK
                     RFASLTAAIEKARAERDAAEERWLELAERLEALNA"
     gene            complement(2564..>4159)
                     /locus_tag="VGB57_02120"
     CDS             complement(2564..>4159)
                     /locus_tag="VGB57_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0130172.1"
                     /translation="PATQATAAERPKLIVAISVDQFSADLFSQYRQRFDGGLQRLSEG
                     VVFPNGYQGHNATETCPGHSTILTGSRPARTGIIANNWFNPSATREDKGVYCSEDPRV
                     PGSSSRGGQYTVSSYHLRVPALGDHMKRADPRSRVAVVSGKDRAAIMMGGYRPDERWW
                     WDHDAKAFVNPGAPATPRAVASANEGVKDALSRARSARELPRVCEPHSRAIPVAGGGK
                     PVGAGRFAREADDKNGFRASPDFDRATLDMGEALRREMKLGEGQATDLLILGLSATDY
                     VGHSFGTQGSEMCIQLMALDEALGGFFRALDSTGIDYLVMLTADHGGHDIPERHREHA
                     AGDAMRVDPALNAGNMAKSLAARLKLSGDVLYGDGAFGDMYVDRKLTAAQRARVLREA
                     IATYRRHPQVAAVFTKAELAAAPGPSGPPDTWSLIQRAKASFDPERSGDFIVLLKPRV
                     TPIFDTSRGYVSTHGSPWDYDRRVPILFWRKGTVPFEQPLAVETADILPTLAATIGVA
                     IPPGSIDGRCLDLDEGPGSSCRR"
BASE COUNT          674 a         1468 c         1404 g          613 t
ORIGIN      
        1 acggccccgg caatgcctcc atagacgatt gcgtatctca ttcgatttcc tcccctcggg
       61 tcggggccgg tataagcggg ccggatcggc ggcgtatatc gcccgaaagg gtgatttgcg
      121 ccgaatgcgg ctaagggatg agggccagcc agcgcgcttt ttcgatcgcc tggacgcgct
      181 tctgcacctc cagcttctcg tagagccgcg cgagatgggt cttcaccgtg ttgggcgaga
      241 ttccaagggc ccgggccagc tccttgttcg actggccgga ggcgagcagc gcgagcagct
      301 cgcattcccg cgcggtgagg cccagcgagc ggatggcggc gtcgttgcgc tcgaagcggg
      361 cgggggccgg ccggggagtc agcctgaggc cggcccagac gcccagcgcg gtgaagccgg
      421 cagcgagcaa ggcgatatat atctcgaccg aaaaagcgtg ggcggcatag cgatattcga
      481 gccattgcag cgccgccgcc gccagcgcca gaaccgcggc gtagaggagg atcgtccggg
      541 tcatggcccc aggctgccag aagtgccgat ccgagaccat cccgtcatcc cggccaaagc
      601 cgggacctca ggacaagaag tctcggcggg cgtcccggag gccccggctt tcgccggggt
      661 gacgaaaccc tccgcagccc ctatctcgcc ccgaccatgg cagcccccgt cctcgcttat
      721 gaaaatctcg gcctcatcca gggctccggc tggctgttcc gcgggctgga cctgttcgtc
      781 ggagcgcgcg accggctggc gctgatcggg cgcaacggcg cgggcaagac gaccctgctc
      841 aaatgcctcg ccggaatcat cgacactgac gagggacgcc ggacgattca gcccggaacc
      901 agggtcgtcc tgctcgagca ggacccggcg gtggatcgct tcgcgaccct tcgcgagttc
      961 gccctccacg gccccgaggc gcccgccccg cacgaggtcg acgccatcgc cgaccagctc
     1021 gggatcgacc tcgaccgctc cgccgccacc gccagcggag gcgagcggcg ccgcgccgcc
     1081 atcgcccggg cgctggccca ggatccggac gtgctcctgc tcgacgagcc gaccaaccat
     1141 ctcgatctcg gcgccatcga atggctggaa gactggctct cccgctacac cggcgccttc
     1201 atcgcgatca gccacgaccg cactttcctg aagcgcctca cccgctcgtc gctgtggctc
     1261 gaccggggcg cgctccgccg cgccgagatc ggcttcggcg gcttcgacgc ctggaccgag
     1321 gcggtctatg ccgaggaggc gcgggcggcg gagaagctcg acgcccggct gaagctcgag
     1381 cttcactggc ttcagcgcgg agtcaccgcc cggcgcaagc gcaaccaggg ccggctcgcc
     1441 aagctgggcg agatgcgcgc ggcccgggcc gcaatgctgg ggccggccgg cgcagccagg
     1501 ctcggcctcg cgaatgacga cgtgaagacc aagatggtga tcgacgccca gaaggtgggc
     1561 aaaagcttcg gcgccggaga gtctcggcgc gcaatcgttc gcgacttcac ccttcgaatc
     1621 cagcgcggcg accgcatcgg ggtggtcggc gccaacggca ccggcaagac gaccctgctg
     1681 aagctcctga ccggcgagct cgaacccgac gagggcaaga tcgcccgggc caagaccctt
     1741 caggggatca tgatcgacca gctgcgccgc ctgctcgatc cagcaaaaag cgtacgcgac
     1801 attcttgccg acggcggcga ctgggtagag gtgcgcgacg aacgcaagca cgtccagggc
     1861 tacttgaagg aattcctgtt cgagccgacg atggtcgacg ccaggatcgg caccctctcg
     1921 ggcggcgagc gctcgcggct cctgctcgcc cgcgagttcg cccgccgctc gaacctgctc
     1981 gtcctcgacg agccgaccaa cgacctcgac ctcgagaccc tcgacctcct gcaggaggtg
     2041 atcgccgatt atgacggcac cgtcctcatc gtcagccacg accgcgactt cctcgacaac
     2101 accgtcacca tcaccctcgg cctcgacggc tcgggcaagg tcgacatcgt cgccggcggc
     2161 tatgaggatt ggcagcgcag gaggcaggat ggtttcccgg cgaaggccgg gacccagtcc
     2221 ggccgcaaga actgggcccc ggcctccgcc ggggagcgaa agcagacgaa gctcacctac
     2281 aaggaccagc gcgactacga cctcctcccc gaccgaatcc aggcgatcga ggcgcagatc
     2341 gcgcgggacg aggaggcgct tcacgatccg gacctctaca cgcgcgaccc gaagcgcttc
     2401 gcctcgctga ccgcggcgat cgagaaggcg cgggcggaac gggatgcggc cgaggaacgc
     2461 tggctggagc tggcggaaag gctcgaggcg ctgaacgcct gaaaagaggg actgtccctc
     2521 ttttctaacg ctccccgccg aaaagaggga ctgtccctct tttttaacgc cggcagctcg
     2581 accccgggcc ctcgtccagg tccaggcacc gcccgtcgat cgagcccggc gggatcgcca
     2641 ccccgatcgt cgccgcgagc gtcgggagga tgtccgccgt ctcgaccgcg agcggctgct
     2701 cgaacgggac cgtccccttg cgccagaaca ggatcggcac ccgccggtcg tagtcccacg
     2761 ggctgccgtg ggtcgagacg tagccccggc tggtgtcgaa gatcggggtc acccgcggct
     2821 tcagaaggac gatgaagtcg ccggagcgct ccggatcgaa gctggccttg gcgcgctgga
     2881 tcaggctcca ggtgtcgggc ggcccggagg gtcccggcgc cgccgcaagc tccgctttgg
     2941 tgaacaccgc cgccacctgg gggtggcgcc tgtaggtcgc gatcgcctcg cgaagcaccc
     3001 gtgcccgctg cgcggccgtc agcttgcggt cgacatacat gtcgccgaac gctccgtcgc
     3061 cgtacagcac gtccccgctc agcttcagcc tggcggcgag cgacttggcc atgttgcccg
     3121 cgttgagcgc cggatccacc cgcatcgcat cgccggcggc atgctcgcgg tggcgctcgg
     3181 gaatgtcgtg gccgccatgg tcggcggtga gcatgaccag atagtcgatc ccggtcgagt
     3241 cgagggctcg gaagaatccg cccagcgcct cgtccagggc catcagctgg atgcacatct
     3301 cgctgccctg ggttccgaag ctgtggccga catagtcggt cgccgagagc cccaggatga
     3361 ggaggtccgt cgcttgcccc tcgccgagct tcatctcgcg ccgaagcgcc tcgcccatgt
     3421 cgagcgtcgc ccggtcgaaa tcgggcgagg cccggaagcc gttcttgtcg tccgcctcgc
     3481 gtgcgaaccg ccccgccccc accggcttgc cgccgccggc gaccggaatc gcccggctgt
     3541 gcggctcgca gacccgggga agctcccggg cggagcgggc gcgggaaagg gcgtccttca
     3601 cgccctcatt ggccgacgcc accgcccgcg gagtcgccgg agcgcccgga ttgacgaatg
     3661 ccttcgcgtc gtgatcccac caccagcgct cgtccgggcg gtagccgccc atcatgatcg
     3721 ccgcccggtc cttgccggag acgacggcga cccggctgcg cggatcggcg cgcttcatat
     3781 ggtcgccgag cgcgggtacc ctgaggtggt aggacgaaac cgtatattgg ccgcctctgg
     3841 agctgctccc cgggacccgc ggatcttccg agcaatagac gcccttgtcc tcccgcgtcg
     3901 cgctcgggtt gaaccagttg ttggcgatga tccccgtccg cgccgggcgc gagccggtga
     3961 ggatggtcga atggccaggg caggtctcgg tggcgttgtg gccctggtag ccgttcggga
     4021 agaccacgcc ctccgacaat cgctgcaggc ccccgtcgaa acgctggcga tactggctga
     4081 acaggtcggc cgagaactgg tccaccgaga tggcgacgat cagctttggc cgttccgcgg
     4141 ctgtggcttg agtggcggg
//
LOCUS       DASWNU010000107         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_289470, whole genome shotgun sequence.
ACCESSION   DASWNU010000107 DASWNU010000000
VERSION     DASWNU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289470"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VGB57_02125"
     CDS             complement(<1..566)
                     /locus_tag="VGB57_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0130173.1"
                     /translation="MTDTTANATHFEAHGAHAAHYGDEHTPGFFVRWFMSTNHKDIGT
                     LYLIFAIVAGIIGGTVSGIMRAELASPGMDFLPAWAGSMDQANHLWNVLITAHGLIMV
                     FFMVMPAMIGGFGNWFVPLMIGAPDMAFPRMNNISFWLLVPAFILLLGSAFVPGGTGN
                     GAGIGWTAYAPLSTSGSSGPAVDMAIFSL"
     gene            complement(568..>1597)
                     /gene="coxB"
                     /locus_tag="VGB57_02130"
     CDS             complement(568..>1597)
                     /gene="coxB"
                     /locus_tag="VGB57_02130"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0130174.1"
                     /translation="APAQPEAMSGAPDNQGEEPSNASVLASSPARTAPDATVGQPTGA
                     MGFQPQVTPIGEEALWFHNVILMPLITAISIFVLALLIFVMVRFRRSANPVPSRNTHN
                     TLLEVLWTLVPVLILVAIAVPSIRLLAHQYSPPKADITVKATGNQWYWTYTYPDHGGF
                     EIVSNGLSDADAKKRGEPRLLAVDERMVVPQGATVKVIITASDVIHSWGVPAFWVKLD
                     AVPGRLNETWFKTDKPGVYYGQCFELCGARHAYMPIAVEVLPPAQFAAWVASKGGTMA
                     GPGGAKAPDATATSPVTNPQQQQQPTPTGGASGATETSPAPPGVTPGTTAPPVSSQGA
                     TNTKRGSN"
BASE COUNT          261 a          490 c          587 g          259 t
ORIGIN      
        1 agcgagaaga tcgccatgtc gacggccggg cccgaggagc ccgatgtcga cagcggcgcg
       61 taagcggtcc agccgattcc ggcgccgttg ccggtcccgc ccgggacgaa ggccgagccg
      121 agcaggagga tgaaggccgg gacgagcagc cagaagctga tgttgttcat ccgcgggaac
      181 gccatgtcgg gcgctccgat catcagcgga acgaaccagt tgccgaagcc gccgatcatc
      241 gccggcatga ccatgaagaa gaccatgatc aggccgtggg cggtgatcag cacgttccac
      301 agatggttgg cctggtccat gcttccggcc caggcgggca ggaagtccat gcccggcgag
      361 gcgagctcgg ctcgcatgat ccccgagacg gtcccgccga tgatccccgc gacgatcgcg
      421 aagatcaggt aaagggtgcc gatatccttg tggttggtcg acatgaacca gcgcacgaag
      481 aagccgggcg tgtgctcgtc gccatagtgg gccgcgtggg cgccatgggc ttcgaaatgc
      541 gtggcgttgg cagtcgtgtc ggtcatgtca gttcgatccg cgtttcgtat tggtggcgcc
      601 ctggcttgag acgggcggag cagtcgtgcc cggagtcacc ccgggagggg ccgggctggt
      661 ctcggtcgcg ccggaagcgc cgcccgtcgg agtcggctgc tgctgctgct gcgggttggt
      721 gaccggcgaa gtggcggtgg catcgggcgc tttggctccg cccggtccgg ccatggtgcc
      781 gcccttcgag gcgacccagg cggcgaactg cgccggcgga agcacctcga ccgcgatcgg
      841 catgtaggcg tggcgggcgc cgcacagctc gaagcactgg ccgtaataga cgcccggctt
      901 gtcggtcttg aaccaggtct cgttgagccg accgggcacc gcgtcgagct tcacccagaa
      961 ggccgggact ccccagctgt ggatgacgtc ggaggcggtg atgatcacct tcaccgtcgc
     1021 gccctggggc accaccatcc gctcgtcgac cgcgagcagg cgcggctcgc cgcgcttctt
     1081 ggcgtcggcg tcgctgaggc cgttggagac gatctcgaag ccgccatggt cgggataggt
     1141 ataggtccaa taccattggt tgccggtcgc cttgaccgtg atgtccgcct tgggcggcga
     1201 atattggtgg gcgaggagtc ggatcgacgg aaccgcgatc gcgaccagga tcagcaccgg
     1261 caccagcgtc cacagcactt cgagcagggt gttgtgggtg ttccgagaag gcaccggatt
     1321 ggcgctgcgc cggaagcgga ccatgacgaa gatgagcaag gcgaggacga agatcgagat
     1381 ggccgtgatg agcggcatca ggatcacgtt gtggaaccac agcgcctcct cgccgatcgg
     1441 agtgacctgg ggctggaagc ccatggcgcc ggtcggctgg ccgacggtcg cgtccggggc
     1501 ggtcctggcc gggctgctcg caagcacgct cgcgttggag ggctcctcgc cctggttgtc
     1561 cggagcgccg ctcatcgcct cgggctgagc gggagcg
//
LOCUS       DASWNU010000108         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_186509, whole genome shotgun sequence.
ACCESSION   DASWNU010000108 DASWNU010000000
VERSION     DASWNU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186509"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGB57_02135"
     CDS             <1..245
                     /locus_tag="VGB57_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130175.1"
                     /translation="RLPGPALGKQLRDIDKRIDRARESGRLSGRDARRLEREARAIGR
                     LARVYGQGGLSPSERAELEARANVLRESVGKPRRGG"
     gene            348..1451
                     /locus_tag="VGB57_02140"
     CDS             348..1451
                     /locus_tag="VGB57_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEY0130176.1"
                     /translation="MFAAILIELELYCELLNLVAHFAGFSVRLADLDDPGEGRRIDSF
                     VADHPEAELFHRPGWTRAIEQGTGQAGRYLIAEDHAGRLTGLLPLTRIRSPLFGSALV
                     STGFGVGGGIVAGTGMAVEALAAAAWSLAERESCPSVTLRGGRLPDGWRREEGVYAGF
                     VRDLPGSDEATLKSIPRKQRAEVRRSLGLGLEVSGGRGRQALDDHYRVYSESVRNLGT
                     PVFPRSLFEAMAREWGEDADILTVHADGRPVAAVLSLYFKGVVHPYWGGGTREARALR
                     ANDLLYYALMRHASARGCTSFDFGRSKTGSGAYAFKKNWGFEPRPLTYATKGAHRDVN
                     PSSPRYRLQTAAWRRLPLPVANRIGPFLARGLG"
     gene            1514..1918
                     /locus_tag="VGB57_02145"
     CDS             1514..1918
                     /locus_tag="VGB57_02145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEY0130177.1"
                     /translation="MKLPHEGVYVRLGLSPVHGIGVFAIRPIPEGTDIFANDGVPLVW
                     VSKAELERADLTPAERALYHDFGINRGESIGCPANFHNLTPGWYLNEPAPGAEANVES
                     TDRLGFIARRDIAEGEELCIDYAGFSEQSEPA"
     gene            complement(2065..2334)
                     /locus_tag="VGB57_02150"
     CDS             complement(2065..2334)
                     /locus_tag="VGB57_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HEY0130178.1"
                     /translation="MAGGWARDGAVQDQIDDTVKDAVLSARARLPAGEGSEYCEDCGD
                     LIPERRREALPGTRTCVPCQAERDEEARRSGAGINRRGSKDSQLR"
     gene            2673..>2915
                     /locus_tag="VGB57_02155"
     CDS             2673..>2915
                     /locus_tag="VGB57_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130179.1"
                     /translation="MANEIAADSLPAQRFRLRLPSRVPDFGPRGFFLFRIVWFAAFAL
                     ALLGPIAGTWHRLAEAGRNSGLVPGSRAGVALAEDDL"
BASE COUNT          414 a         1054 c          974 g          473 t
ORIGIN      
        1 gccgcctacc cggcccggcg ctcggcaagc aattgcggga tatcgacaaa cgcatcgacc
       61 gcgcccgcga gtccggccgc ctctccggcc gcgacgcccg ccggctcgag cgcgaggccc
      121 gcgccatcgg ccgcctcgcc cgggtctacg gccagggcgg cctgtcgccc tcggagcggg
      181 ccgagctgga ggcgcgggcc aatgttctgc gcgaatcggt cggcaagccc cggcgcggcg
      241 ggtgaagcaa atcgggacag tgattttctc gagcttgtcg gcgcgcagcg gcccggccta
      301 acgcttcagc ccaacctcag tcgatcccgg ggcggaggcc cggcgccatg ttcgctgcca
      361 tcctgatcga gctggaactc tattgcgagc tcctgaacct cgtcgcccat ttcgcggggt
      421 tcagcgttcg gctggcggat ctggacgatc ccggcgaggg ccggcggatc gattccttcg
      481 tcgccgatca tcccgaggcg gaactcttcc atcgcccggg ctggaccagg gcgatcgagc
      541 aggggaccgg ccaggccggg cgctatctca tcgccgaaga ccatgccggg cgattgacgg
      601 gattgcttcc gctcacccgg atccgctccc cgcttttcgg ctccgctctg gtttcgaccg
      661 gcttcggggt cggcggcggc atcgtcgccg gcacgggcat ggcggtcgag gcgctggccg
      721 ccgccgcctg gtcgctggcg gagcgggaaa gctgcccctc ggtgacgctt cgcggcggcc
      781 ggcttccgga cggctggcgg cgggaggaag gagtctatgc cggcttcgtg cgagacctgc
      841 ccgggagcga cgaggccacc ctgaaatcca tcccgcgcaa gcagcgcgcc gaggtgcggc
      901 gctcgctcgg gctcgggctg gaggtgagcg ggggccgcgg tcggcaggcg ctcgacgacc
      961 attaccgggt ctattccgaa agcgtccgca atctcggcac tccggtcttc ccgcgcagcc
     1021 tgttcgaggc gatggcccgg gaatggggtg aggatgccga catattgacg gtccatgccg
     1081 acggcaggcc ggtcgctgcg gtgctcagcc tttatttcaa gggggtcgtc catccttatt
     1141 ggggcggcgg cacccgcgag gcccgggcgc tgcgcgccaa cgacctcctc tattacgcgc
     1201 tgatgcgcca cgcctcggca cgcggctgca cgagcttcga tttcggccgc tccaagaccg
     1261 gatccggggc ctatgcgttc aagaagaatt ggggcttcga gccgcggccg ctcacttatg
     1321 cgaccaaggg cgcgcaccgc gacgtcaacc cgtccagccc ccgctaccgc ctccagaccg
     1381 ccgcctggcg gcgccttccg ctgccggtcg cgaaccggat cgggccgttc ctcgcccgag
     1441 ggctcggctg agggggcatt gcggatcctg ccgtcacggc tagtcaggtc gcggccgcaa
     1501 ggagatcgtc gccgtgaaat tgccccacga aggcgtctac gtccgcctcg gtctgtcgcc
     1561 cgttcacggc atcggcgtgt tcgcgatccg gcccatcccg gaggggaccg acatcttcgc
     1621 caacgacggc gtacccctcg tctgggtgag caaggcggag ctcgagcggg ccgatctcac
     1681 cccggccgaa cgcgctctct accacgattt cggaatcaac cgcggcgaga gcatcggctg
     1741 cccggccaat ttccacaacc tgacccccgg ctggtatctc aacgagccgg cgcccggcgc
     1801 cgaggccaat gtcgaaagta ccgaccgcct cggcttcatc gcccgccgcg acatcgccga
     1861 gggcgaggag ctgtgcatcg actatgcggg gttcagcgag cagtccgagc cggcctagct
     1921 accccgcccg tcctttccgc gaacgcgggt atccaaccgg cggtggagcc cccgcctttc
     1981 cggaagcagt acgtcagtgt ctccgcatcc cgcgccgctg atcaagggcg atacctcgcg
     2041 tcgcggcacg agcgcgcggg caggtcagcg caactggctg tccttgctcc ccctgcggtt
     2101 gatccccgcg cccgatcgcc gcgcctcctc gtcgcgctcc gcctgacagg gaacgcaggt
     2161 cctcgtcccc ggcaacgcct cgcggcgccg ctccgggatg aggtctccgc aatcctcgca
     2221 atattcggag ccctcgcccg ccggcagcct cgcccgggcg ctcagcaccg catccttgac
     2281 cgtgtcgtcg atctgatcct gaacggcgcc gtcgcgcgcc catccgccgg ccatggcgaa
     2341 gctcctgtct gtcaaagcct cagctaaaat aggaagcccg gcccttcgtt ccaggcgagc
     2401 gccgcccggc ctccgcgggg cggcgccgat agcggctata ggacgaagtg ggcgagtaca
     2461 ctgcacccct ccgaccggca ggcgtctccc tgaaaagaac ttataccgct cccatttttc
     2521 gcctggagtg cgacgacaac gccgagcgtc gccagccaag ctgggcgggt tcgtaagcta
     2581 ccgaggggga acgttcgcga ttttcccgat ggccgccgcc tcgccccatg ctatcgaacc
     2641 ggggggtcgt ttcagactgg ggcgcttgcg ggatggcaaa cgagatcgca gccgatagcc
     2701 tgccggccca gcgctttcgg ctgaggctgc ccagccgggt gccggatttc gggccgcgcg
     2761 gcttcttcct gttccggatc gtctggttcg ccgcgttcgc gctcgccttg ctcgggccga
     2821 tcgccggcac ctggcaccgg ctcgcggagg cggggcgcaa cagcggtctc gtccccggaa
     2881 gccgtgccgg cgtcgctttg gccgaagacg atctg
//
LOCUS       DASWNU010000109         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_108320, whole genome shotgun sequence.
ACCESSION   DASWNU010000109 DASWNU010000000
VERSION     DASWNU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108320"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="VGB57_02160"
     CDS             <1..1442
                     /locus_tag="VGB57_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HEY0130180.1"
                     /translation="TATVYNKGAELIRMLHTLLGPERFRAGSDLYFDRHDGQAVTCED
                     FVRAMEEATGEDLSQFRLWYEQAGTPRVRAELAEENGAARLRLEQEVPPTPGQPVKKP
                     MPIPLRVALFDRSSGEKVEERLILHDKARDEALFSARRDEVVLSINRGFSAPVIVETE
                     RPAADLAFLSAHDDDPFARYEAMQQLMLDTLLAAIGSGRGDHRAVLDAVRQTLADEAL
                     DPAFTADAVLPPSESFIGDQMAVVDPEAIRTARDALRAELGRALEPQWRAAYASTAAN
                     RYEYSPAAKGARRLRTVALNYLMAAGAEDAPALARRQYDQADNMTDRQGALGALASSD
                     SPERNAALAAFYERYRADPLVLDKWFTVQALSTRHDTLAAVEALAGHPDFTLSNPNRM
                     RSLVGAFAANQRAFHDASGRGYRFLADMILAVDRLNPQTAARLVPPLGRWRRFDSGRQ
                     DLMKTELERIVATPGLSKDVFEQASKSLG"
     gene            1680..2351
                     /locus_tag="VGB57_02165"
     CDS             1680..2351
                     /locus_tag="VGB57_02165"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0130181.1"
                     /translation="MGEIEPEGSAEDRIAPRTNLLLAATAEVGGRHLPVRIRNLSDTG
                     AMVEGAGLPEAGMPLILMRGDLQVAATVAWAAGGRRGVRFSGPTPVNEWTGGKPRAID
                     CTGLRDQRRVDAIQAEARASPVSGRGLRSSEGPATHPLVSDLDKRLADELGYVQRLLE
                     GLGDELIADLLLIQRHGKSLQSLDLVGQILGHVSTILRSDDKAAVVEDIGMEDLRARL
                     KRKPI"
     gene            complement(2439..>2899)
                     /locus_tag="VGB57_02170"
     CDS             complement(2439..>2899)
                     /locus_tag="VGB57_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY0130182.1"
                     /translation="SSRTAPAGEAITVVPPTAPAELNTPRVPVRTGATQVAYVKDAQW
                     VEVPTILFARLVSETIAAKTGRVVLDPRQFTFDPGQRLTGTLQTFGLDADRMEAVLVY
                     DAAVSRGKESVETRRFEARVPVAALDAASAPAALNQAANQVAAEVAAWVG"
BASE COUNT          450 a         1019 c         1015 g          415 t
ORIGIN      
        1 acacggcgac cgtctacaac aagggcgccg agctgatccg gatgctccac accctgctcg
       61 ggccggagcg gttccgggcc gggagcgacc tctatttcga ccggcacgac ggccaggcgg
      121 tgacctgcga ggatttcgtc cgggcgatgg aagaggcgac cggcgaggat ctgtcccaat
      181 tccgcttgtg gtacgagcag gcggggactc cgcgggtgcg ggccgagctg gccgaagaga
      241 atggggcggc gcgcctccgg ctcgagcagg aagtgccgcc tactccgggc cagcccgtga
      301 agaagccgat gccgatcccg cttcgggtcg ccctgttcga ccggagcagc ggggagaagg
      361 tcgaggagcg gctcatcctg cacgacaagg cgcgggacga ggccctgttc agcgccaggc
      421 gcgacgaggt cgtcctctcg atcaaccgcg gcttctcggc gccggtgatc gtcgagaccg
      481 agcggccggc ggcggacctc gccttcctct cggcgcatga cgacgatccc ttcgcccgtt
      541 acgaggcgat gcagcagctg atgctcgaca ccctgctggc ggcgatcggc tcgggccggg
      601 gcgaccatcg cgcggtgctg gacgcggtgc gccagaccct tgccgacgag gcgctcgacc
      661 ccgcgttcac ggccgacgcc gtgcttccgc cgagcgaatc gttcatcggc gaccagatgg
      721 cggtggtcga cccggaggcg atccgcacgg cgcgcgacgc gctccgcgcc gagctcggcc
      781 gggcgctgga gccgcaatgg cgggcggcct atgcctcgac cgccgccaat cgctacgaat
      841 attcgcccgc ggccaagggt gcccggcggc tcaggacggt ggcgctcaac tatctgatgg
      901 cggccggggc cgaagatgcc ccggcgctcg cccgccggca atatgaccaa gcggacaaca
      961 tgaccgatcg tcagggggcg ctcggagcgc tggcgagctc ggattcgccg gagcgcaacg
     1021 ccgcgctcgc cgctttctac gagcgctacc gcgccgatcc cctggtgctc gacaaatggt
     1081 tcaccgtgca ggcgctctcc acccgccacg acacgctcgc ggcggtcgaa gccctggccg
     1141 ggcaccccga cttcaccctc tccaatccca accgcatgcg ctcgctggtc ggggccttcg
     1201 ccgccaacca gcgcgccttc cacgacgcct cgggccgcgg ctaccgcttc ctcgccgaca
     1261 tgatcctcgc ggtcgaccgg ctcaatccgc agaccgcggc ccggctggtt ccaccgctcg
     1321 gccgctggcg ccgcttcgac tccggccgcc aggatctgat gaagaccgag ctcgaacgca
     1381 tcgtcgccac gccggggctg agcaaggacg tgttcgagca ggccagcaag tcgctgggct
     1441 gagcggaaga gaggcaggcc gtcatcccgg gagggacccc ttcaaagtag tactttgaag
     1501 ggaacccgaa agccgggacc tcgggaagac ccgccgagac ttctcgtccc gagtcccggc
     1561 ttgcgccggg atgacgcgct ttccctctgt ctctaagtcc gaattaacca ttgcaggtta
     1621 agcctttacc ggaccgcatt ggggcgcggg tcaccggagg cggaaccgac gtgggggaga
     1681 tgggggagat agagccggaa ggcagcgccg aggaccggat cgcccctcgc acaaatttgc
     1741 tgctcgcggc gacggccgag gtcggcgggc ggcacctccc ggtgcggatc cgaaacctct
     1801 cggacaccgg agccatggtc gaaggcgcgg gactgcccga ggccgggatg ccgctgatcc
     1861 tgatgcgcgg cgacctccag gtcgcggcga ccgtcgcctg ggccgcaggc ggccgccgcg
     1921 gagtccgctt ctcggggccg actccggtca acgaatggac gggcggcaag ccccgggcga
     1981 tcgactgcac cggccttcgc gaccagaggc gggtcgacgc gattcaggcc gaagcccggg
     2041 ccagcccggt ttccggccgg gggctgcgct cctccgaagg tccggccacc caccccctgg
     2101 tctcggatct cgacaagcgg ctggcggacg agctcggcta cgtgcagcgc cttctggagg
     2161 gccttggcga cgagctcatc gccgacctcc tcctcatcca gcgccacggc aagtcgcttc
     2221 agagcctcga cctggtcggc cagatcctcg gccacgtctc cacgatcctg cgctccgacg
     2281 acaaggccgc cgtggtcgaa gacatcggaa tggaggattt gcgcgcgcgg ctgaagagga
     2341 agccgatcta accgtcatcc cggcgaaagc cgggaccccg cggagacccg ccgggacttc
     2401 ttgtcctgag gtcccggctt tcgccgggat gacgcggatc agccgaccca ggccgccact
     2461 tcggccgcga cctggttggc cgcctggttc aaggccgccg gggcgctcgc ggcgtcgagg
     2521 gcggcgaccg ggacccgggc ttcgaagcgc cgggtctcga ccgattcctt gccgcgcgag
     2581 acggcggcgt catagacgag caccgcttcc atccggtcgg cgtcgaggcc gaaggtctgg
     2641 agcgttccgg tcaggcgctg accgggatcg aaggtgaatt gcctgggatc gagaacgacc
     2701 cggcccgttt tcgcggcgat cgtctcggag accaggcggg cgaacaatat ggtcggcacc
     2761 tccacccatt gcgcgtcctt gacgtaggcg acctgggtgg cgccggtgcg caccggcacc
     2821 cgcggcgtgt tgagctcggc cggagccgtc ggcgggacga cggtaatcgc ctcgccggcc
     2881 ggcgcggtcc ggctcgatg
//
LOCUS       DASWNU010000110         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_238126, whole genome shotgun sequence.
ACCESSION   DASWNU010000110 DASWNU010000000
VERSION     DASWNU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238126"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VGB57_02175"
     CDS             <1..786
                     /locus_tag="VGB57_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEY0130183.1"
                     /translation="DAWRHSVATGEPYDIEHRVRMVDGSHRWARSRAYPRRRADGEIC
                     LWYGSTEDVHERKVAEEHQRLLINELNHRVKNTLASVQGIAFQTLRGEVSLAEARARF
                     EARLIALSGAHNLLTEENWGGASLERVVSDSIEHLAGETGRFDVDGAPLRLVPRAALA
                     LAMALHELGTNASKYGALSTDGGRVSIGWTRDGDRLHLVWRESGGPPVEPPSRRGFGS
                     RLIERGLAADLGGTAALSFDPDGLRCDIQASLAAIRAPEQALG"
     gene            779..1144
                     /locus_tag="VGB57_02180"
     CDS             779..1144
                     /locus_tag="VGB57_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0130184.1"
                     /translation="MAERRLRILVVEDEMLVAMNIEDMLLELGHEVAGLASRLGPALA
                     LAGENRFDAAMLDVNLAGEPSFPVADLLAERGIPFLFATGYGRAGIEERFRACPILQK
                     PFRAAELGAALAALSYPAA"
     gene            complement(1159..1851)
                     /locus_tag="VGB57_02185"
     CDS             complement(1159..1851)
                     /locus_tag="VGB57_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0130185.1"
                     /translation="MAAHNNRMSDERNSVTQAILGRRSARAFLPRPVERVLVEELLEL
                     AARAPSGGNLQPWRVDVLTGEPLAALKAEALASTAAEMELKVYPSPLPEPYRTRRYRS
                     GEALYATIGIGREDRAARLAQFARNFEAFGAPVLLLFSIDRIFDRPQWAHLGMFIQNL
                     MLLAHERGLASCPQEAWAAVAGVVAEHIELPAERILYCGMALGYADGDAPINALRTER
                     EPVDEFARFRGF"
     gene            1850..3091
                     /locus_tag="VGB57_02190"
     CDS             1850..3091
                     /locus_tag="VGB57_02190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY0130186.1"
                     /translation="MRLLDKMLPGLIKRGELTVIDPAGRTRRFGAPDPELRPVTVRMM
                     DRRVAFQIARDPAVGTAETWMDDRLRIEQGDILDLVRIIRRNRRWEERSRPNGFTKKT
                     GRLRHRLDMLNWKSRSLKNVQHHYDVGNDLYRLFLDPDMQYSCAYFTDPANSIEQAQL
                     DKKAHIASKLLLEPGQKVLDIGCGWGGLALYLNRVADVDVLGVTLSKEQLAVARQRAE
                     EAGVSDRVKFELIDYRDVTGQFDRIVSIGMFEHLGPPFFRTFFKKVRELLKPEGVSLI
                     HTMGRMGKPGTTDRFMLKYIFPGGYLPALSEIVGASEREKLIMSDCEALRLHYIHTLV
                     AWYERFKAKEAEITAMYDRRFYRLYAFYLAASSTMFSDGAMVVYQLQYLRSRYAAPMT
                     RDYMLKDEARLREAQGPPPLG"
BASE COUNT          536 a         1132 c         1067 g          503 t
ORIGIN      
        1 gacgcctggc gccacagcgt cgccaccggc gagccctacg acatcgagca tcgggtccgg
       61 atggtcgacg gctcccatcg ctgggcccgc tcgcgcgcct atccccgccg ccgcgccgac
      121 ggggagatct gcctgtggta cggctcgacc gaggacgtgc acgagcgcaa ggtcgcggag
      181 gagcatcagc gcctcctgat caacgagctc aaccaccggg tgaagaacac cctcgccagc
      241 gtccagggca tcgctttcca gaccttgcgc ggcgaggtgt cgctcgccga ggcccgggcc
      301 cggttcgagg cccggctgat cgcgctgtcc ggcgctcaca acctgctcac cgaggagaat
      361 tggggcggcg cgtccctgga gcgggtggtg agcgactcga tcgagcacct cgccggggag
      421 accggacggt tcgatgtcga cggagcgccg ctccggctgg tcccgcgcgc cgccctcgct
      481 ctggccatgg cgcttcacga gctcggcacc aacgcctcca aatatggagc gctttcgacc
      541 gacggcggcc gggtgtcgat cggctggacc cgggacggcg accggcttca cctcgtctgg
      601 cgggaaagcg gcggcccgcc ggtcgagccg ccgagccggc gcggcttcgg ctcccggctg
      661 atcgagcgcg gcctcgccgc cgatctcggc ggaaccgccg cgctgagttt cgacccggac
      721 ggcttgcgct gcgacatcca ggcctcgctc gccgcgatcc gggcgccgga gcaggctctt
      781 ggctgagcgc cgactcagga tcctcgtcgt cgaggacgag atgctggtgg cgatgaatat
      841 cgaggacatg ctgctcgagc tcggccacga ggtcgcgggc ctggcgagcc gcctcggccc
      901 cgctctggcc ctggccggcg agaaccggtt cgacgcggcg atgctcgacg tcaacctcgc
      961 cggcgagccg agcttcccgg tcgccgacct gctcgcggag cgcggcatcc ccttcctgtt
     1021 cgccaccggc tacggccgcg ccgggatcga agagcggttc cgcgcctgcc cgatcctgca
     1081 aaagccgttc cgcgccgcgg aactgggcgc ggccttggcg gccttatcct acccggccgc
     1141 gtagcgggtg gggaggattt agaacccccg aaacctcgcg aactcgtcca ccggctcccg
     1201 ctccgtccgg agcgcgttga tcggcgcatc gccgtcggca tagcccagcg ccatcccgca
     1261 atagaggatc cgctccgccg gcagctcgat atgctccgcc acgacgcccg ccaccgccgc
     1321 ccatgcctcc tgagggcagc tggcgaggcc gcgctcgtgc gccagcagca tcaggttctg
     1381 gatgaacatc cccagatgcg cccattgcgg ccggtcgaag atccggtcga tcgagaacag
     1441 cagcagcacc ggtgccccga acgcctcgaa attgcgggcg aattgcgcaa gccgggcggc
     1501 gcggtcctcg cgcccgatgc cgatcgtcgc ataaagcgcc tcgccgctcc gataccggcg
     1561 ggtccggtag ggctcgggca gcggcgaggg atagactttc agctccatct ccgccgcggt
     1621 cgaagccagc gcctccgcct tgagcgccgc gagcggctcg ccggtcagga cgtccacccg
     1681 ccagggctgg aggttgccgc ccgacggcgc ccgcgcggcg agctcgagca gctcttcgac
     1741 cagaacgcgc tcgaccggcc gcggcaggaa agcacgggcc gaacggcgtc ccagaatggc
     1801 ctgggtgacg gagttgcgct cgtcggacat gcggttattg tgggcggcca tgcggctact
     1861 cgacaagatg cttccggggc tcatcaagcg gggcgagctc accgtcatcg atcctgccgg
     1921 ccggacccgc cgcttcggcg cccccgatcc ggagctgagg ccggtcaccg tccggatgat
     1981 ggaccggcgg gtggcgttcc agatcgcccg cgatccggcg gtgggcaccg ccgagacctg
     2041 gatggacgat cggcttcgga tcgagcaggg cgatatactc gatctcgtcc gcatcatccg
     2101 ccgcaaccgg cgctgggagg agaggagccg gccgaacggc ttcaccaaga agaccggaag
     2161 gctccgccac cgcctcgaca tgctgaactg gaagtcgcgg tcgctgaaga acgtccagca
     2221 ccattatgac gtcggcaacg acctctaccg gctcttcctc gatccggaca tgcagtacag
     2281 ctgtgcctat ttcaccgatc ccgccaacag catcgagcag gcccagctcg acaagaaggc
     2341 ccatatcgcc tccaagctgc tgctcgagcc cggccagaag gtgctcgaca tcggctgcgg
     2401 ctggggtggg ctggccctct atctgaaccg ggtcgccgac gtcgacgtgc tcggagtcac
     2461 cctttccaag gagcagctcg ccgtcgctcg gcagcgggcc gaggaggcgg gggtctcgga
     2521 ccgggtgaag ttcgagctga tcgactatcg cgacgtcacc ggccagttcg accggatcgt
     2581 ctcgatcgga atgttcgagc atctcggccc gcccttcttc cggaccttct tcaaaaaggt
     2641 gcgcgagctt ctgaagccgg agggggtctc gctgatccac acgatgggcc ggatgggaaa
     2701 gcccggaacg accgaccggt tcatgctcaa atacatcttt cccggcggct atcttccggc
     2761 cttgtcggag atcgtcggcg ccagcgagcg cgagaagctg atcatgtcgg actgcgaggc
     2821 gctccgcctc cactacatcc acaccctcgt cgcctggtac gaacgcttca aggcgaagga
     2881 agccgagatc acggcgatgt acgatcgccg cttctaccgc ctctacgctt tctacctcgc
     2941 cgcgtcgtcg accatgttct ccgacggcgc catggtcgtc taccagcttc aatatctgcg
     3001 cagccgctat gccgcgccca tgactcggga ttacatgctg aaggacgagg cgcggttgcg
     3061 cgaagctcag gggccgccgc cgctcggtta gaggcggcgg cttgaccgaa tggcgggagc
     3121 catgctcatt gccgcaaaaa tcggggaaca ggacgaatgt ggctgctcga caaattgctg
     3181 aaaaagatcg tcaccaaagg cgagctcacc gtcatcgacc atgacggcaa ggaatatc
//
LOCUS       DASWNU010000111         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_55997, whole genome shotgun sequence.
ACCESSION   DASWNU010000111 DASWNU010000000
VERSION     DASWNU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55997"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VGB57_02195"
     CDS             <1..730
                     /locus_tag="VGB57_02195"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0130187.1"
                     /translation="LVLECTGFFTDRDKAGAHLTAGAKKVLISAPAKGVDLTVVYGVN
                     HDRLEAGHTIVSNASCTTNCLAPVAKVMNDTIGIERGLMTTVHAYTNDQKILDQIHSD
                     KRRARAAAMSMIPTTTGAARAVGEVLPELKGKLDGSAIRVPTPNVSLVDLTFTPKRDT
                     SLEEVNGALRAAATSGPLAGILDYSEEPLVSIDYNHCPASSTVDSLETAVLEGKLVRV
                     LSWYDNEWGFSNRMVDTAGAMARL"
     gene            833..2026
                     /locus_tag="VGB57_02200"
     CDS             833..2026
                     /locus_tag="VGB57_02200"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0130188.1"
                     /translation="MTAFKTLDDLGDVHGRRALVRVDLNVPMADLEVTDDTRLRATLP
                     TVTELADKGAIVLLLAHFGRPKVPSPEYSLSLVTRAYQAVLGRPVRYVDWEVAEENVA
                     MLQPGDVAILENTRFFGGEEKNDPAVAQRFARLGDLYVNDAFSAAHRAHASTEGLAHL
                     LPAYAGRAMEAELKALQKALGEPERPVAAVVGGAKVSSKLEVLRHLVTKVDHLIIGGG
                     MANTFLAARGVDVGKSLCEHELAPTALEILDAAERASCTVHLPYDVIVAKEFAPNPPS
                     LRTCNVHEVAADEMILDVGPAAVEALADALKTCRTLVWNGPLGAFETPPFDRATVQLA
                     QTAAALTQAGQLVSVAGGGDTVAALNHAGVADGFTFVSTAGGAFLEWMEGKELPGVKA
                     LEAQS"
BASE COUNT          358 a          746 c          668 g          321 t
ORIGIN      
        1 cctcgtcctg gaatgcaccg gcttcttcac ggatcgcgac aaggccggtg cgcatcttac
       61 tgcgggcgcg aagaaagtcc tgatctcggc tccggccaag ggagtcgatc tcaccgtcgt
      121 ctacggagtc aatcacgacc ggctcgaggc cgggcacacc atcgtctcca acgcgtcctg
      181 cacgaccaat tgcctggcgc ccgtcgccaa ggtgatgaac gacacgatcg ggatcgagcg
      241 cggcctgatg acgacggtcc acgcctatac caacgaccag aagatcctcg accagatcca
      301 ttccgacaag cgccgggccc gcgccgccgc gatgtcgatg atcccgacca cgaccggcgc
      361 cgcgcgcgcc gtcggcgagg tgctgcccga gctgaagggc aagctcgacg gctcggcgat
      421 ccgggttcca acgcccaacg tcagcctcgt cgacctcacc ttcacgccca agcgcgacac
      481 cagcctcgaa gaggtcaacg gcgccctccg ggccgccgcg acaagcgggc cgctcgccgg
      541 aatcctcgac tatagcgaag agcccctggt ctcgatcgac tataaccatt gtccggccag
      601 ctcgaccgtc gacagcctcg agacggccgt gctcgagggc aagctcgtcc gggtcctttc
      661 ctggtacgac aatgaatggg gcttctcgaa ccggatggtc gacaccgccg gcgcgatggc
      721 gaggctgtga caaggctcgt catgccggcg aaagccggca cctcggggag acccgccgag
      781 acttctcgac ctggggtccc ggctttcgcc gggatgacgg gtcggtgacg tcatgaccgc
      841 gttcaagacc ctcgacgacc tcggcgacgt ccacggcagg cgggcgctcg tccgggtcga
      901 tctcaacgtc ccgatggcgg atctggaggt gacggacgac acccggctgc gcgccacact
      961 gccgacggtg accgagctcg ccgacaaggg cgcgatcgtc ctcctcctcg cccatttcgg
     1021 ccggcccaag gtaccgagcc cggaatattc gctttcgctc gtcaccaggg cctaccaggc
     1081 cgtgctcggc cgtccggtcc gctacgtcga ctgggaggtg gcggaagaga atgtcgccat
     1141 gctccagccc ggcgatgtcg cgatcctcga gaacacccgc ttcttcgggg gcgaggagaa
     1201 gaacgacccc gccgtggctc agcgtttcgc acgccttggc gatctctacg tcaacgacgc
     1261 cttctccgcc gcgcatcgcg cccatgcctc caccgagggg ctggcgcatc tgcttcccgc
     1321 ttatgccggg cgggcgatgg aagccgagct gaaggcgctc cagaaggcgc tgggcgagcc
     1381 ggagcggccg gtggccgcgg tcgtcggcgg cgccaaggtc tcgtccaagc tcgaggtgct
     1441 ccggcatctg gtgaccaagg tcgaccacct catcatcggc ggcggaatgg cgaacacctt
     1501 cctcgccgcc cgcggggtcg atgtcggcaa gtcgctgtgc gagcacgagc tcgccccgac
     1561 cgccctcgag atcctcgacg cggccgagcg ggcgagctgc accgtccatc tcccctacga
     1621 cgtcatcgtg gcgaaggaat tcgcccccaa cccaccgagc ctcaggacct gcaacgtcca
     1681 cgaggtcgcg gccgacgaga tgatcctcga cgtgggaccc gcggccgtcg aggcgctggc
     1741 cgacgccctc aagacctgcc ggaccctggt ctggaacggg ccgctcggcg ccttcgagac
     1801 cccacccttt gaccgagcca ccgtccagct cgcccagacc gccgcggctt tgacccaggc
     1861 cggtcagctc gtctccgtgg ccggcggcgg cgacacggtg gcggcgctca accatgccgg
     1921 agtcgccgac ggcttcacgt tcgtctcgac cgccggcggg gcgttcctcg aatggatgga
     1981 gggcaaggag ctgccggggg tgaaggcgct ggaagcacaa tcatgacggt ccgatctacc
     2041 cacattcagt cggtgatcgt cttactggat ctgcttgagc gacatgagca aaa
//
LOCUS       DASWNU010000112         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_212167, whole genome shotgun sequence.
ACCESSION   DASWNU010000112 DASWNU010000000
VERSION     DASWNU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212167"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1556
                     /locus_tag="VGB57_02205"
     CDS             <1..>1556
                     /locus_tag="VGB57_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130189.1"
                     /translation="GEDTASYASSNASTGVAVSLAGASATRGDAVGDMFEEVENLTGS
                     AGHDELGGDGLDNELTGGLGNDVLRAEAGDDTYIWSRGDGNDTIEEGIVAFEEAMTAE
                     GALLGGFLPAISHRTEDYSGYMEHWHTLEIRKGSETVYSYSFVGPNPYNTTELDAWPT
                     DGWLGSYRATGVGSQAVMNSLSTASDAGSDILELGEKISLSHLSFEAAGADLIIHVAP
                     DETGAGAGQIRIRNHYTTGGKVETLQFHDGASVSLKSFEIGRSVGLEGVVDDLLIGSD
                     GSDVLRGEAGNDAFFGGAGLNELRGGSGDDLFEAGTGKDMYFGEAGAEDTVRFVASAA
                     AVNVNLGTGTGAGGSADTDTFSEIENVTGSAHGDILTGNQFDNELIGLGGDNILTGND
                     GNDVLTALGGNDTLNGGEGEDALSGGDGTDTLIGGGGKDFLDGGAGGGTLDGGIGDDQ
                     IVGGSGVDTIRGGEGADILNGRGGGDTLQGDQGDDQYVFEPGTGTDTITDGSGKNGIH
                     FGGGIDRSQL"
BASE COUNT          301 a          514 c          542 g          199 t
ORIGIN      
        1 ggcgaggaca ccgcctccta cgcctcgtcc aacgcctcga ccggagtcgc cgtaagcctc
       61 gccggcgcct ccgccacccg cggcgacgcg gtcggcgaca tgttcgaaga agtcgagaac
      121 ctaaccggca gcgccggcca cgacgagctg ggcggggacg gcctggacaa cgagctcacc
      181 ggcggcctcg gcaacgacgt gctgcgcgcc gaggcgggcg acgacacgta catctggagc
      241 cgcggcgacg gcaacgacac catcgaggaa gggattgtcg cgttcgagga ggcgatgacc
      301 gcggaaggcg cgcttctcgg tgggttcctc ccggcgatca gccacaggac cgaagactat
      361 agcggatata tggagcactg gcacacgctc gagatccgca aggggtccga gaccgtgtac
      421 agctacagct tcgtgggacc caatccgtac aatacgacgg aattggacgc ctggcccacc
      481 gacgggtggc tcggcagcta ccgtgcgacg ggggttgggt cccaggcggt catgaactcc
      541 ctttcgacgg cgagcgacgc cggcagcgac attctcgaac tcggcgagaa gatcagcctc
      601 agccacctga gcttcgaagc cgccggcgcc gatctcatca ttcatgtcgc gccggacgag
      661 acgggagccg gcgcgggcca gatcaggatc cgcaatcatt atacgaccgg cgggaaggtc
      721 gagactctcc agttccacga cggcgcttcg gtcagcctga agagcttcga gatcggtcgc
      781 agcgtcggct tggagggggt ggttgacgac ctcctgatcg gcagcgacgg aagcgacgtc
      841 ctcaggggcg aggccggcaa cgacgccttc ttcggcggcg cggggctgaa cgagctgcgc
      901 ggcggaagcg gcgacgacct gttcgaagcc ggtaccggca aggacatgta cttcggagaa
      961 gcgggcgccg aggacaccgt gcgcttcgtg gcctcggccg cggccgtgaa cgtgaacctg
     1021 ggcaccggaa ccggcgccgg cggaagcgcc gacaccgaca ctttctccga gatcgagaat
     1081 gtcaccggct cggcccatgg cgacatcctc accggcaacc agttcgacaa cgagctgatc
     1141 gggctcggcg gcgacaacat cctcaccggc aacgacggca acgacgtgct caccgcgctc
     1201 ggcggcaacg acacgctcaa cggcggcgaa ggcgaagacg cgctttcggg cggcgacggc
     1261 accgacaccc tgatcggcgg cggcggcaag gatttcctcg acggcggcgc cggcggcgga
     1321 acgctggacg gcggcatcgg cgacgaccag atcgtcggcg gcagcggcgt cgatacgatt
     1381 cggggcgggg agggcgccga catcctcaac ggccgcggcg gcggcgatac gctgcagggc
     1441 gaccaaggcg acgaccaata tgtcttcgag cccggcaccg gcaccgacac gatcaccgat
     1501 ggatccggca agaacggcat tcacttcggc ggcggcatcg accgcagcca gctctg
//
LOCUS       DASWNU010000113         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_186588, whole genome shotgun sequence.
ACCESSION   DASWNU010000113 DASWNU010000000
VERSION     DASWNU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186588"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VGB57_02210"
     CDS             complement(<1..891)
                     /locus_tag="VGB57_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0130190.1"
                     /translation="MLLSRILRLPSNDMAIDLGTVNTVVYVRGKGIVLNEPSVVAIET
                     VDGVRRVKAAGRDAKLMMGKTPEHIQAVRPLRDGVIADIDVAEQMIKHFIDRAHGGPS
                     RLGRHPEIVVCVPSGSTSVERRAIRDAATNAGASKVWLIEEPMAAAIGAGLPVTEPVG
                     AMVVDIGGGTTEVAILSLHGLAYSTSIRVGGDKMDDAITAAVRRKHNLQIGEATAERI
                     KLEVGSALPPGGEAATIRVRGRHLAAGMPQEIFISEAEIADAIAEPVSHIIGAVRTAL
                     ENTAPELAADIVDRGIVLTGG"
     gene            1288..>1522
                     /locus_tag="VGB57_02215"
     CDS             1288..>1522
                     /locus_tag="VGB57_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130191.1"
                     /translation="MESGERPVELRVAAGPSPWPAMILDAKFIEKVPDSMNFVNFAGK
                     SPCFQSSCFPPRCDGIKSRLHPRPHPGRQENQPR"
BASE COUNT          234 a          516 c          503 g          269 t
ORIGIN      
        1 gcccccggtc agcacgattc cccgatcgac gatgtcggcg gcgagctccg gagcggtgtt
       61 ctcgagcgcg gtgcggaccg ctccgatgat gtggctcacc ggctcggcga tggcgtcggc
      121 gatctcggct tcgctgatga agatttcctg aggcatcccg gccgccaaat ggcgcccgcg
      181 cacgcgtatc gtcgcggcct cgcctccagg cggaagcgcg cttccgacct cgagcttgat
      241 gcgttccgcc gtcgcttcgc cgatctggag gttgtgcttg cggcggacgg cggcggtgat
      301 cgcgtcgtcc atcttgtccc cgccgacgcg gatggaggtg ctgtaggcca ggccgtgcag
      361 ggacaggatc gccacctccg tcgtccctcc gccgatgtcc acgaccatcg ctccgaccgg
      421 ttccgtcacc ggcagcccgg cgccgatcgc cgccgccatc ggctcctcga tcaaccagac
      481 cttcgaggcg ccggcattgg tcgccgcgtc gcggatcgcc cggcgctcca cggacgtcga
      541 gccggagggg acgcagacga cgatttccgg atggcgcccc aagcgggagg ggccgccatg
      601 ggcccggtcg atgaaatgct tgatcatctg ttcggcgaca tcgatgtcgg cgatcactcc
      661 gtcgcgcagc ggccggaccg cctggatgtg ctcgggcgtc ttgcccatca tgagcttggc
      721 gtcgcggccc gccgccttca ctcgccggac gccgtcgacc gtctcgatgg cgacgaccga
      781 aggctcgttg agcacgatcc ccttccctcg gacgtagacg acggtattca ccgttcccag
      841 gtcgatggcc atgtcgttcg aggggagacg caggattcgc gaaagcagca tggcaggata
      901 tcgtcctcat cgcgaaaaac gggagagctg gccgatttgg ccgaagcgga gccgcgaatc
      961 tcgcggcctc cgatccgcgg agcctacatc atagtttccg gtctttagaa gcttgaaata
     1021 tatgcggtcg ctcgaggatt cacattctgc tcgccggggc ggacggcgag ggaggtgccg
     1081 ccggccgctt catccgtgtc gcccttcggt cgcgatccct ggcgatcgga ccgcgcgccc
     1141 ctggatgagc agaggcttgg catgggccgt cccggtatcg agccccgcgc aggcgacaag
     1201 gctgtcccga acgtcgcgtt tctggtatgt tccatcccta tgtgcccggc ccgaaggccg
     1261 gatgtcgcgg cggaaaggag ggcacccatg gagtccgggg agcggccggt cgagcttcgg
     1321 gtcgcggcgg ggccaagtcc ctggccggca atgattctcg acgcgaagtt cattgaaaag
     1381 gtgcccgact cgatgaactt cgtgaacttc gccggaaaaa gcccgtgttt tcagtcgtcg
     1441 tgcttcccgc cgcggtgcga cgggatcaag tcacgccttc accctcgccc ccacccaggc
     1501 cgccaagaga accagccccg cc
//
LOCUS       DASWNU010000114         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_56021, whole genome shotgun sequence.
ACCESSION   DASWNU010000114 DASWNU010000000
VERSION     DASWNU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56021"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VGB57_02220"
     CDS             complement(<1..766)
                     /locus_tag="VGB57_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0130192.1"
                     /translation="MAKSRKRYVCQACGSVQNRWMGQCPDCTEWNTLVEDAGAAVTPF
                     SARHNLRGGGRAIELVGLDRDVALPERMSTGIREFDRAIGGGLVAGSATLIGGDPGIG
                     KSTLLLQAAALMAKAGLAVAYISGEEATDQVRLRARRLGLGGAPVQLAAATSVRDVLT
                     TLGEGAPPALLVIDSIQTMYSDLIEGAPGTVSQVRASAGELIRYAKESGAAVVLVGHV
                     TKDGAIAGPRVLEHMVDTVLAFEGERSHQYRILRATK"
     gene            1010..1333
                     /locus_tag="VGB57_02225"
     CDS             1010..1333
                     /locus_tag="VGB57_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY0130193.1"
                     /translation="MFIQIHADNQIDSDNERDSRLEEQIRQRLARFEGRITDVEVHVS
                     DVNGPRGGNADLRASIEARVNGIPPVAAIDEGDTVDRAVIGAAKKVVRALDHQLGKLN
                     DRHRH"
     gene            1484..>1969
                     /locus_tag="VGB57_02230"
     CDS             1484..>1969
                     /locus_tag="VGB57_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0130194.1"
                     /translation="MIRVASYNMRKAIGTDRRRRPERTLEVLRELDADVIALQEADRR
                     FGTRASAIPLHMIDEHSDYKPVPFDARDGSLGWHGNALLVRKNVDILHKELLHLPSLE
                     PRGAVMADISLDGEALRVIGMHLDLSGLWRRQQAHFILHHLQTRQAEMPAVLMGDLNE
                     WS"
BASE COUNT          347 a          662 c          664 g          296 t
ORIGIN      
        1 tcttggtcgc cctgaggatc cggtactgat ggctccgctc gccttcgaac gccaggaccg
       61 tgtcgaccat atgctcgagc actcgcggcc cggcgatggc gccgtccttg gtgacgtggc
      121 cgacgaggac gacggcggca ccggattcct tggcgtagcg gatgagctcg ccggcggagg
      181 cccggacctg gctgacggtt ccgggcgcgc cttcgatcag gtccgaatac atggtctgga
      241 tcgaatcgat gacgagcaag gccggcggcg ccccctcccc cagcgtcgtc agaacgtcgc
      301 ggaccgaggt ggcggcggcg agctggaccg gcgctccgcc gaggccgagg cggcgggcgc
      361 ggagccgcac ctggtcggtc gcctcctcgc cggaaatgta ggcgacggcg agccccgcct
      421 tcgccatcag cgccgccgcc tggagcagaa gggtcgactt gccgattccg ggatcgccgc
      481 cgatcagggt ggccgagccg gcgacgaggc cgccgccgat cgcccggtcg aactcgcgga
      541 tccccgtcga catgcgctcg ggcaaagcga cgtcgcgatc gaggccgaca agctcgatcg
      601 cccgaccgcc gccgcgcaga ttgtgccgcg ccgagaaggg cgtgaccgcc gctccggcat
      661 cctcgacgag cgtgttccac tctgtgcagt cggggcattg gcccatccag cgattctgga
      721 cggagccgca ggcctggcag acgtagcgct tcctggattt ggccatgggg ggagacgtag
      781 cgcgggaagg tgcgaaacgg aaggggaacg ttatcttgcc tccccatgcc cagcatgggg
      841 agggggacca cccaaagggt ggtggagggg taattggcag aagcctccgc cggctacccc
      901 tccaccatgc ttcgcatggt ccccctcccc atcttgcgat ggggaggcaa aaaagtcgga
      961 accccctccg ctccccggca ttcactccat gccagcattg gagagtgttt tgttcatcca
     1021 gatccacgcc gacaatcaga tcgacagcga caacgaacgc gacagccgac tcgaggagca
     1081 gatccgccag cgcctcgccc gcttcgaagg gcggatcacc gacgtcgagg tccatgtctc
     1141 ggacgtcaac ggaccgcgcg gcggcaatgc cgatttgcga gcctcgatcg aggctcgggt
     1201 gaacggcatc ccgcccgtcg ccgccatcga cgagggcgac acggtcgacc gagccgtgat
     1261 cggcgccgcc aagaaggtgg tgcgggcgct cgaccaccag ctcggcaagc tcaacgaccg
     1321 gcaccggcac taggccgggg cctcaggaac agtcactttc cgaacgggcc gggtagaatt
     1381 cgcatccgcc gtgcgggaaa gtgactgtcc cctccagcgc cggttacttt ccgtaaaatc
     1441 ggcggctaat gagcatcgcc gtgcgagtga gtaaccgtcc ccgatgatcc gcgtcgccag
     1501 ctacaatatg aggaaggcga tcggcaccga tcgccggcgg cggccggagc ggacgctcga
     1561 agtgcttcgc gagctcgatg ccgacgtcat cgccctgcag gaggccgacc ggcgcttcgg
     1621 cacccgggcg agcgccatcc cgctgcacat gatcgacgag catagcgact acaagcccgt
     1681 ccccttcgac gcccgcgacg gcagcctggg ctggcacggc aatgcgcttc tggtcaggaa
     1741 gaatgtcgac atccttcaca aggaactgct ccacctcccc tcgctcgagc cgcgcggagc
     1801 ggtgatggcg gacataagcc tcgatggcga ggcgcttcgg gtgatcggga tgcacctcga
     1861 cctgtcgggg ctgtggcggc ggcagcaggc ccatttcatc ctccaccatt tgcagacgcg
     1921 ccaggcggag atgccggcgg tgctgatggg cgatctgaac gaatggagc
//
LOCUS       DASWNU010000115         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_4573,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000115 DASWNU010000000
VERSION     DASWNU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4573"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VGB57_02235"
     CDS             <1..1305
                     /locus_tag="VGB57_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0130195.1"
                     /translation="LNTGCVPSKALIRSARAAHEIRTAAAYGIAPAEPEVRFAEVMER
                     VHRIIAEIAPADSVERYTRLGVDVRKGHGRIVDPWTVEIDGGERLTARAIVIAAGGEP
                     AVPDIPGLAESGFLTSDSMWGKLRGRETVPARIAIVGGGPIGTEMAQAFARLGAKVTQ
                     VEHGPRLLPKEDEDASALVEAVLREEGVDIRTGHDAVRVEGKVLIVRSDKEYAIPFDE
                     IIVAVGRKARLTGYGLEDLGIETGRTVAVNEWLETLYPNIYAVGDVAGPYQFTHFAAH
                     QAWFAAVNGLFGHLKRFRADYRVLPWVTFTDPEVAHVGHNALSAKEAGIDFEVVRYEL
                     THLDRALAEGANRGFVKLLVAPGKDRLLGATIVAANAGELLPELVLAMKHGLGLNKLL
                     ATIHAYPTLAEANKYAAGEWKKAHKPERLLAWLERYHAWRRG"
     gene            1302..>1719
                     /locus_tag="VGB57_02240"
     CDS             1302..>1719
                     /locus_tag="VGB57_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY0130196.1"
                     /translation="MTRIVIFAKAPVAGRVKTRLIPALGAEGAAVLARAMLERTVEEA
                     LATGLGVELCGEPDAAQWHEERPGLSLAGQGEGDLGARLARAAARVLAEDSILLIGAD
                     CPELDRDRLRAAADALEDHDAVIHPAHDGGYALLGLR"
BASE COUNT          270 a          582 c          617 g          250 t
ORIGIN      
        1 ctcaacaccg gctgcgtgcc ctccaaagcc ctgatccgaa gcgcccgcgc cgcccacgag
       61 atccgcaccg ccgccgccta cggaatcgcc cccgccgagc cggaggtgcg cttcgccgag
      121 gtgatggagc gagtgcaccg gatcatcgcc gagatcgcgc ccgccgacag cgtcgagcgc
      181 tacacccggc tcggggtgga cgtgcgcaag ggccatgggc ggatcgtcga tccctggacg
      241 gtcgagatcg acggcggcga gcggctcacc gcccgcgcca tcgtcatcgc cgcgggcggc
      301 gagcccgccg tgccggatat tccaggcctg gcggaaagcg gcttcctcac cagcgactcg
      361 atgtggggga aattgcgcgg ccgcgagacg gtgccggcgc ggatcgcgat cgtcggcggc
      421 ggccctatcg gaaccgagat ggcgcaggcc tttgcccggc tgggggcgaa ggtgacccag
      481 gtggagcatg gcccgcggct cttgccgaag gaggacgagg acgcctccgc gctggtcgag
      541 gcggtgcttc gcgaggaggg ggtggatatc cgaaccggcc acgacgcggt gcgggtcgag
      601 ggcaaggttc tgatcgtccg atcggacaag gaatacgcca tccccttcga cgagataatc
      661 gtcgcggtcg gccgcaaggc gcggctgacc ggctatgggc tggaagatct cggaatcgag
      721 accggccgga ccgtcgccgt caacgaatgg ctggagacgc tctaccccaa tatctatgcc
      781 gtcggcgacg tcgccgggcc ctaccaattc acccatttcg cggcccacca ggcctggttc
      841 gcggcggtca acggcctgtt cggccatctg aagcgcttcc gcgccgatta ccgggtgctg
      901 ccctgggtga ccttcaccga tccggaggtc gcccatgtcg gccataacgc gctttccgcg
      961 aaggaggcgg ggatcgactt cgaggtcgtg cgctacgagc tgacccacct cgatcgggcc
     1021 ttggccgaag gggcgaaccg gggcttcgtc aaattgctcg tcgcgcccgg caaggaccgt
     1081 ctgctcggcg ccaccatcgt cgcggccaat gcgggcgagc tgctccccga gctggtgctg
     1141 gcgatgaagc acggcctcgg cctcaacaaa ttgctcgcca ccatccacgc ctatccgacc
     1201 ctcgcggagg ccaacaaata tgccgccggc gaatggaaga aggcgcacaa gcccgagcgg
     1261 ctgctcgcct ggctggagcg ctatcacgcc tggcggcgcg ggtgacccgg atcgtcatct
     1321 tcgccaaggc accggtcgcg ggacgggtga agacccggct catcccggcg ctgggcgcgg
     1381 aaggcgccgc ggtgctggcg cgggcaatgc tggagcggac tgtggaggag gccctggcga
     1441 cggggctcgg ggtcgagctt tgcggggagc ccgatgccgc gcaatggcac gaagaacgcc
     1501 cgggcctttc ccttgccggt caaggcgagg gagacctggg cgcgaggctc gctcgggcgg
     1561 ccgcccgggt gcttgccgag gacagtatcc tgcttatcgg cgccgactgc cccgagctcg
     1621 accgggaccg gcttcgggcg gcggcggatg ccttggagga ccatgacgcc gtcatccacc
     1681 cggcgcatga cggcggctat gcgctgctcg ggcttcggc
//
LOCUS       DASWNU010000116        10546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_82052, whole genome shotgun sequence.
ACCESSION   DASWNU010000116 DASWNU010000000
VERSION     DASWNU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82052"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VGB57_02245"
     CDS             <1..960
                     /locus_tag="VGB57_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0130197.1"
                     /translation="QDPGFSRALTNGGVTSLHVLPGSANLFGGRGVTLKNVYARTMQG
                     MKFPGAPYSLKMACGENPKRVYGGRNQMPGSRMANIALMRQTWARAQEYRRKWDNYER
                     NGGEMPGRDLALDTLRGVLAGEITVQNHCYRADEMANIMDMAKEFGYKVTAFHHAVES
                     YKIADLLKAHGACSAMWADWYGFKMEAYDGIQENIPLVHNAGACAIVHSDDPNGIQRL
                     NQEAAKALANGRRIGIQVSDEVAWTWLSFNPAKALGIGDRTGSLRTGKMADVVLWNGH
                     PFSVYTRPERVWVDGALMYDANDPRRRPVSDFELGQPGEGDVK"
     gene            1065..2450
                     /locus_tag="VGB57_02250"
     CDS             1065..2450
                     /locus_tag="VGB57_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0130198.1"
                     /translation="MTACALALLASPALAQTIAITGGTVALGDGSEPIEGGTVVVQNG
                     RIVAAGRDVAVPAGAQRIDATGRWVTPGIVAGFSRVGLVEVDAVNATNDVSAANSPFS
                     AAIDVAPAINPRAMPIQVNRAGGVTRAIVAPGTAKSIFAGQGAVIDLGNDMEPITRAR
                     AFQFVELGETGAEEAGGSRAAAYVLFRNALREARDLGGRFSAPISGGGGAREPRANDQ
                     RDFPLEEVPDNRLLSAGANRPDDVLLTRFDAAALVPVLQGRQLLLVHVERASDILQVL
                     ALKKEFPRLRLVLVGATEGWTIADRIAAARVPVIANALNDLPSTFEQLAATQSNVARL
                     RAAGVPVALGMINDDEARQVRLARQQAGNMVAVGRVPGASGLSWGEALATITSAPAEA
                     VGMGGEIGSLRPGRRADVVVWSGDPFENSSAAEIVLIDGVQQPLDNHQTRLRDRYRNA
                     SEGDLPKAYER"
     gene            2450..3139
                     /locus_tag="VGB57_02255"
     CDS             2450..3139
                     /locus_tag="VGB57_02255"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEY0130199.1"
                     /translation="MATVSLALATLAFVGTHLAMSHPLRTRLVQNLGDQWFSALYSAV
                     SFITLGWMILAYRASENSYFLWVSPDWAEPVAAVVTLIACILLAGSFFRNPAFPHPGA
                     KPRAPRAASGVFAVTRHPMNMAFILWALAHIALWGALPNLIVAGGILVLAMVGSIGQD
                     HKKLNVIGEPWRAWMGRTSFVPFGALLSGRAKWSALAEAWPALLIGVALWLAVTWLHA
                     PEVSPIAALTR"
     gene            complement(3184..4560)
                     /locus_tag="VGB57_02260"
     CDS             complement(3184..4560)
                     /locus_tag="VGB57_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY0130200.1"
                     /translation="MQGRILPPLPPPPLRRYTRSMSQATRILAALAAGLLLGILTADL
                     GWAPRAADLVEPVGQIWLRGLQMTIVPLVVALLVTGIAESAEAARGSRVAGRSFVLMV
                     VILWLSAIFAALFVPLLLRLFPMGAEAAGALSSALTTAKPVDTVPGFGDFLRSIVPSN
                     PVAAAASDQILPLVVFATVFAFALTRIPAEGRGRVVDLFRAIAEAMIVIVNWLLWLGP
                     IGVFALAYVVGVRAGDDAFTALLHYIAIVSSVGLVILVLAYPFAIAGARLPLGRFARA
                     VAPAQAVGFSTQSSLASLPAMLKSSERLGVPVAASGVTLPLAVALFRATGPGMNMAVV
                     LYIAHWYGMSLSPAQIAMGVVVAAITTFSAISLPGQVSFVTNTAPIALAMGVPLEPLA
                     LLIAVETVPDLFRTVGNVTMDVAVAATVARRSGLDEAEMSEEDRLLQDGETASRHPGE
                     GRDLGETR"
     gene            4726..6060
                     /gene="glmM"
                     /locus_tag="VGB57_02265"
     CDS             4726..6060
                     /gene="glmM"
                     /locus_tag="VGB57_02265"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0130201.1"
                     /translation="MTRKFFGTDGIRGRTNQNPMTAEMAQRVGQAAGAHFLRGAHRHR
                     VVIGKDTRLSGYMMETALISGFTSVGMDVVMVGPVPTPAVAMLTRSMRADLGVMISAS
                     HNPYTDNGIKLFGPDGYKLSDEDESAIEALLDAAPNLVDAPLIGRSRKIEDARGRYIH
                     FAKATFPEELRLDGLKIVVDCANGAAYQVAPSALWELGADVVAMGVSPNGLNINDGCG
                     STAPAALQARVVEEGAHIGIALDGDADRLIIVDEVGRIVDGDQLMALIAISFARRGLL
                     DGDGVVATVMSNLGLERFLATNGLELVRTQVGDRYVLEAMRERGFNVGGEQSGHIILS
                     DYSTTGDGLIAALQVLACLVEDGRPASELLHQFDPLPQLLKNVRFGGGAPLESAGVKS
                     AIQSAEQRLRGVGRLVIRKSGTEPLIRVMAEGEDEAMVEEVVDSICEAVKAA"
     gene            6166..6393
                     /locus_tag="VGB57_02270"
     CDS             6166..6393
                     /locus_tag="VGB57_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY0130202.1"
                     /translation="MLELRPDCERCGRDLPPESAEARICTFECTFCADCVEGPLGGVC
                     PNCGGNFQPRPIRPAGKLEKYPASMLRKGPK"
     gene            6482..7360
                     /gene="thiD"
                     /locus_tag="VGB57_02275"
     CDS             6482..7360
                     /gene="thiD"
                     /locus_tag="VGB57_02275"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY0130203.1"
                     /translation="MTVPRILIIAGSDSGGGAGIQADIKTVTMLGGHAMTAITAITAQ
                     NTLGVEAVMPVPTEIVLRQIEAVLADLGADAIKIGMIGSAETAAAIASRIEALHDIPI
                     VFDPVMVATSGSVLADEATIAGFERLMRLSTLVTPNLPELETLAGAELSGEDEIVAAA
                     AALYREHGCRVLAKGGHSAGGRIDDFLIWEKGTIAHHSDRVDTLHTHGTGCTLASAIA
                     TGLGGGLPLDRAVDRGLHYVHAAILAAPGFGRGNGPIGHSLATTPYHHLKAAQEGGEW
                     GVETWDSVWSPGGTRH"
     gene            7435..8079
                     /locus_tag="VGB57_02280"
     CDS             7435..8079
                     /locus_tag="VGB57_02280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY0130204.1"
                     /translation="MARPCFKLEKVYPDPVAGVDEAGCAPLAGPVVAAAVILDREKFP
                     RGIDDSKALPAATREAICAKLYKVARIGVGLATVEEIDEINIYWARMLAMTRAVDALG
                     LDPAMILVDGNRCPKWERPSVSIVAGDTKCRSIAAASIVAKVTRDRIMLDYAREHPGY
                     GWETNKGYPTPQHYRALDSLGPTPLHRRSFAPVRQVMMNLAHAVEAQAADSGDR"
     gene            complement(8174..9634)
                     /locus_tag="VGB57_02285"
     CDS             complement(8174..9634)
                     /locus_tag="VGB57_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130205.1"
                     /translation="MAASLLSNLAVWLTAAAAQAEDPRRIDPVQPSETASYPDAAEVA
                     RGSGIGEAEAASRLRMQEVSAPHVSKLRREFADRLAGLYLESEGDYRLVVRLKGPAPV
                     ADRWIGGGRDRMRIQFLAGAPATLAETLEKVAAAMPAVGARLPGLMGTGVDERTGEVM
                     LNVYAVGEDVERARASEPELERLLGHPVRLEFSSTRMTDGQGSARPLPRPPAPGTAEW
                     SSHMQGLARSARHPGYPHPGILDFQRESRALARRVQADPAFAGWVFKNDNEPHAVVLF
                     TGDAAAHLARYTSDPRYWPKSVGLTRPQLRALQDSYGKLLRQLEIHYTFSSGDEEKNQ
                     VTFSISEMDKYRRAVAEARLKPHPLVVIVQDAGPIARVAQSAAPATHFPQFRFPGRLM
                     EAALTGTLEVRNGCLMVGDTLVLWPSNARLSLDRDGVPVVGGGRDRPLRVGEQVRISG
                     GTAWTDFRGEGLLQPLPAACKGEAWDSDTLWIAGEF"
     gene            9924..>10546
                     /locus_tag="VGB57_02290"
     CDS             9924..>10546
                     /locus_tag="VGB57_02290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0130206.1"
                     /translation="MGSRAKSASPPVPDPALPLNQILQEDCIAAMARLPAGSVDMVFA
                     DPPYNLQLGGDLFRPEGGLVDAVDDDWDKFATFAAYDEFTRAWLKEARRILKPNGTLW
                     VIGSYHNIYRVGAALQDAGYWILNDIIWRKSNPMPNFRGTRFTNAHETLIWASRSEDS
                     RYTFNYRAMKALNDELQMRSDWLLPICSGGERVKDGGTKAHPTQKPE"
BASE COUNT         1746 a         3516 c         3687 g         1597 t
ORIGIN      
        1 caggacccgg gcttcagccg ggcgctgacc aacggcgggg tgaccagcct gcacgtgctt
       61 ccgggatctg cgaatttgtt cggcggacga ggggtaacgc tgaagaacgt ctatgcgcgg
      121 accatgcagg ggatgaagtt cccgggcgcg ccttattcgc tgaagatggc ctgcggcgag
      181 aatcccaagc gggtctatgg cggccgcaac cagatgccgg gcagccggat ggccaacatc
      241 gccctgatgc ggcagacctg ggcgcgggct caggaatatc ggcgcaagtg ggacaattac
      301 gagcgcaacg gcggcgagat gcccggccgc gaccttgcct tggacaccct gcgtggagtg
      361 ctcgccggcg agatcacggt ccagaaccat tgttaccggg ccgacgagat ggccaacatc
      421 atggatatgg cgaaggagtt cggctacaag gtcacggcct tccaccatgc cgtcgagagc
      481 tacaagatcg ccgatcttct gaaggcgcac ggcgcctgct cggcgatgtg ggccgactgg
      541 tacggcttca agatggaagc ctatgacggc atccaggaga atatcccgct ggtccacaat
      601 gccggcgcct gcgcgatcgt ccattccgac gatcccaacg gcatccagcg gctcaaccag
      661 gaagcggcca aggcgctcgc caacggccgg cggattggca tccaggtgtc ggacgaggtc
      721 gcctggacct ggctcagctt caacccggcc aaggcgctcg gcatcggcga ccggacgggc
      781 agcctgcgca ccggcaagat ggccgacgtg gtgctttgga acggccatcc gttcagcgtc
      841 tacacaaggc ccgaacgggt ctgggtggac ggagccctga tgtacgacgc caatgacccg
      901 aggcgccgcc cggtcagcga tttcgagctc ggccagccgg gtgaagggga tgtgaagtga
      961 aggggctgtt ccaaacccgt catgccggac ttgatccggc atccatgaac acggtgggac
     1021 ggaggttcga gcgccggcgt tcatggatcc cggctttcgc cgggatgacg gcgtgcgcac
     1081 tggccctcct cgcctctccc gctctggccc agacgatcgc gatcaccggc ggcaccgtcg
     1141 cgctcggcga cggctcggag ccgatcgagg gcggaacggt ggtcgtccag aacgggcgca
     1201 tcgtcgccgc cgggcgcgac gtcgcggttc cggccggcgc tcaaaggatc gacgcgaccg
     1261 gcagatgggt cactccgggg atcgtcgccg gcttctcgag ggtcgggctg gtcgaggtcg
     1321 acgcggtcaa cgcgaccaac gacgtctccg cggccaactc gccgttcagt gccgcgatcg
     1381 acgtcgctcc ggcgatcaat ccgcgcgcca tgccgatcca ggtcaaccgt gccggaggag
     1441 tcacccgcgc catcgtcgct ccgggcaccg cgaagagcat cttcgccggg cagggcgccg
     1501 tcatcgacct gggcaacgac atggagccga ttacccgggc ccgggccttc cagttcgtcg
     1561 agctgggcga gaccggggcg gaggaggccg gcggaagccg cgccgccgct tatgtgctgt
     1621 tccgcaacgc attgcgcgaa gcgcgagacc tcggcggccg cttctcggct ccgatctccg
     1681 gcggcggcgg cgcgcgcgag cctcgggcga acgaccagcg cgacttcccg ctcgaggaag
     1741 tgccggacaa ccgcctcctc tccgccggcg caaaccggcc cgacgacgtt ctgctcaccc
     1801 gcttcgacgc ggccgccctg gtgccggtgc tccaggggcg gcagctgctc ctcgtccatg
     1861 tcgagcgggc cagcgacatc ctccaggtgc ttgcgctgaa gaaggaattc ccgcgcctca
     1921 ggctggtcct ggtcggagcg accgaaggct ggaccatcgc agaccggatc gccgcggctc
     1981 gggtgccggt gatcgccaac gcgctcaacg atctgccctc gaccttcgag cagctggcgg
     2041 cgacccagtc gaacgtcgcc cgcctccgcg ccgcgggagt ccccgtggcg cttggaatga
     2101 tcaacgacga cgaggcccgg caggtgcggc tcgcccggca gcaggcgggc aacatggtcg
     2161 cggtcgggcg ggtgcccgga gcctcgggcc tgagctgggg cgaggcactg gcgacgatca
     2221 cctcggcgcc ggcggaagcg gtcggcatgg gcggcgagat cggctcgctc aggccggggc
     2281 gccgcgccga cgtcgtcgtc tggtcgggcg acccgttcga gaacagcagc gccgccgaga
     2341 tcgtcctgat cgacggggtc cagcagccgc tcgacaatca ccagaccagg ctccgcgacc
     2401 gctaccgcaa tgcgagcgaa ggcgatcttc cgaaagccta cgagaggtga tggccacggt
     2461 cagcctcgcg ctggcgaccc tggccttcgt cggcacccac cttgccatgt cgcatccgct
     2521 gaggacccgg ctcgtccaga atctcgggga ccagtggttc tcggcccttt attcggcggt
     2581 gtcgttcatc accctgggct ggatgatcct cgcctacagg gcgagcgaga acagctattt
     2641 cctgtgggtg tcgcccgact gggcggagcc ggtcgcggcg gtagtcaccc tcatcgcctg
     2701 catcctcctc gccgggtcct tcttccgcaa tccggccttc ccgcacccgg gagccaagcc
     2761 cagggcgccg cgggcggcga gcggggtgtt cgcggtcacc cgccatccga tgaacatggc
     2821 cttcatcctg tgggcgctgg cccatatcgc cttgtggggc gccttgccca atctgatcgt
     2881 cgcgggcggg atcctggtgc tcgccatggt cggctcgatc ggtcaggacc acaagaagct
     2941 caacgtgatc ggcgagccgt ggcgggcctg gatggggcgg acgtcgttcg tgccgttcgg
     3001 ggccctgctc tccggccggg cgaaatggag cgctctggcg gaagcctggc cggccctgct
     3061 catcggcgtg gcgctgtggc tggccgtcac ctggctccac gcgcctgaag tctctccgat
     3121 cgcggcgctg acgcggtagc tctcgtcatc ccggcgaaag ccgggacctc gtgacgagaa
     3181 gtctcagcgg gtctccccga ggtcccggcc ttcgccggga tgacgactgg cggtctcccc
     3241 gtcctgcaaa agccggtcct cctcgctcat ctccgcctcg tccaggccgc ttcggcgggc
     3301 gacggtggcg gcgacggcca cgtccatcgt cacgttgccc acggtgcgga agagatcggg
     3361 gacggtttcg accgcgatca gcagcgcgag cggctcgagc ggcacgccca tggcgagcgc
     3421 gatcggcgcg gtattggtga cgaagctcac ctggcccggc aggctgatgg cgctgaaggt
     3481 cgtgatggcc gcgacgacga cgcccatggc gatctgcgcc gggctgaggc tcatcccgta
     3541 ccaatgggcg atgtagagga cgacggccat gttcatgccc ggtccggtcg ccctgaacag
     3601 agcgacggcg agggggaggg tgactcccga ggcggcgacg gggaccccga ggcgctccga
     3661 ggatttgagc atcgccggca ggctggccag cgacgactgg gtgctgaagc cgaccgcctg
     3721 ggccggcgcc acggctcggg cgaaacggcc gagcgggagg cgcgcgccgg cgatggcaaa
     3781 cggataggca agcaccagga tgacgaggcc gacggaggaa acgatcgcga tatagtggag
     3841 cagggcggtg aaggcgtcgt cgccggcgcg aacgcccacg acataggcga gggcgaaaac
     3901 gccgatcggc cccagccaga gcagccagtt gacgatcacg atcatcgctt cggcgatcgc
     3961 ccggaagagg tccaccaccc ggccgcgccc ttcggcggga atgcgggtca gggcgaaggc
     4021 gaaaacggtc gcgaagacga cgagcggaag gatctggtcg gaggccgcgg ccgcgaccgg
     4081 gttggacgga acgatcgacc tcaggaagtc gccgaagccc gggaccgtat cgaccggctt
     4141 ggccgtggtg agggcggagc tgagggctcc cgccgcctcg gcgcccatcg ggaacaggcg
     4201 cagcaacagc ggcacgaaca aggccgcgaa gatcgcggag agccagagga tcacgaccat
     4261 cagaacgaac gagcggcccg cgacccggct gccgcgcgcg gcttcggcgc tttcggcgat
     4321 gccggtgacg agcagagcca cgacaagcgg aacgatcgtc atctgaagcc cccgcagcca
     4381 gatctggccg accggctcga ccagatcggc ggcgcgcggc gcccacccca ggtcggcggt
     4441 gaggatgccg agcaggagcc cggcggcgag tgcggcgagg atgcgggtgg cttgcgacat
     4501 cgagcgggtg tagcggcgga gcgggggagg gggaagcggc ggaagaatcc tcccctgcat
     4561 ttgcagggga gggggaccat gcgaagcatg gtggaggggt cttgctgagt ccgaagaacc
     4621 cctccaccgc gcttcgcgcg gtccccctcc ccgagaaatc tcggggagga atttaactca
     4681 ggcgcctctc gcgctaaggc ggggcaacaa agcgcaagga tcgccatgac tcgcaaattc
     4741 ttcggcaccg acgggatcag gggccgcacc aaccagaatc cgatgacggc ggagatggcg
     4801 cagcgcgtcg gccaggccgc cggggcgcat ttcctgcgcg gcgctcaccg ccaccgggtc
     4861 gtgatcggca aggacacgag gctgtccggc tatatgatgg agacggcgct catctccggc
     4921 ttcaccagcg tcggaatgga cgtggtgatg gtgggcccgg tgccgactcc ggccgtggcg
     4981 atgctgaccc gctcgatgcg cgccgacctc ggcgtgatga tctcggccag ccataacccc
     5041 tacaccgaca acggcatcaa attgttcggg cccgacggct acaaattgtc ggacgaggac
     5101 gagtccgcga tcgaggcgct gctcgacgcc gcgcccaatc tggtcgacgc gcctctgatc
     5161 ggccgctctc gcaagatcga ggatgcgcgg ggccgctaca tccatttcgc caaggcgacc
     5221 ttcccggagg agcttcgcct ggacgggctc aagatcgtcg tcgattgcgc caacggcgcc
     5281 gcctatcagg tcgccccttc ggccttgtgg gagctgggcg cggacgtggt ggcgatgggg
     5341 gtctccccca acggcctcaa catcaacgac ggctgcggct cgaccgctcc cgccgcgctt
     5401 caggcgcggg tggtggagga gggcgcgcat atcggcatcg ccctcgacgg cgacgccgac
     5461 cggctgatca tcgtcgacga ggtgggccgg atcgtcgacg gcgaccagct gatggctctg
     5521 atcgccatct ccttcgcccg gcgcgggctt ctcgacggcg acggcgtggt ggcgacggtg
     5581 atgtccaacc tcggcctgga gcgtttcctc gccaccaacg ggctcgagct ggtgcgcacc
     5641 caggtcggcg accgctatgt cctggaagcg atgcgcgagc gcggcttcaa cgtcggcggc
     5701 gagcaatcgg ggcacatcat cctgtccgat tactcgacca ccggcgacgg cctcatcgcc
     5761 gcgctccagg tgctcgcctg cctggtcgag gacggccgcc cggcgagcga gttgctccac
     5821 cagttcgatc cgctgcccca gctgctcaag aacgtgcgct tcgggggcgg agcgccgctg
     5881 gaatcggccg gggtcaagtc cgcgatccag tcggccgagc aacggctccg gggcgtcggg
     5941 cgcctcgtca tccgcaagtc cggcaccgag ccgctgatcc gggtcatggc cgaaggcgag
     6001 gacgaggcga tggtcgagga ggtggtcgac agcatctgcg aggcggtgaa ggcggcgtag
     6061 gtctccgtca tgccggactt gatccggcat ccaccttctg ttgcagggcc gggcgaggca
     6121 aggcggaccc cggatcaagt ccggggtgac gaaggagcga agaagatgct tgagcttagg
     6181 ccggactgcg agcgctgcgg gcgcgatttg ccgcccgaga gcgctgaggc gcgcatctgc
     6241 acgttcgaat gcaccttctg cgccgactgt gtcgaagggc cgctgggcgg ggtctgcccc
     6301 aattgcggcg gcaacttcca gccgcggccg atccggcccg ccggcaagct ggagaaatac
     6361 ccggcctcga tgctgcgcaa ggggccgaag tgaccacccc caccgtcatt gcgagcgaag
     6421 cgaagcaatc cagcggctta ggcggtggaa gctggattgc ttcgtcgcta cgctcctcgc
     6481 aatgacggtc ccgcgcatcc tcatcatcgc cggctccgac agcggcggcg gcgcgggaat
     6541 ccaggccgac atcaagacgg tgacgatgct cggcggccac gccatgacgg cgatcaccgc
     6601 gatcaccgcg caaaataccc tgggggtcga ggccgtgatg ccggtcccca ccgaaatcgt
     6661 gcttcggcag atcgaggcgg tgctcgcgga cctcggggcc gacgcgatca agatcgggat
     6721 gatcggatcc gccgaaacag cggcggcaat cgcgagcagg atcgaggcgc tgcacgacat
     6781 cccgatcgtc ttcgacccgg tcatggtcgc cacaagcggc tcggtcctgg ccgatgaggc
     6841 cacgatcgcg ggcttcgagc gcctgatgcg gctctctacg ctggtcactc ccaatcttcc
     6901 cgagctcgag acgctggcgg gcgccgaatt gtccggggag gacgagatcg tggcggcggc
     6961 ggcggcgctt taccgggaac atggttgccg ggtcctcgcc aagggcgggc attcggcagg
     7021 cgggaggatc gacgatttcc tcatctggga aaagggcacc atcgcccatc attcggaccg
     7081 cgtggacacc ctccacacgc atggaaccgg ctgcacgctc gcaagcgcga tcgcgacagg
     7141 gctgggtggc gggctgcccc tcgatcgcgc cgtggatcgc ggccttcact atgtgcacgc
     7201 cgccattctc gccgcacccg gcttcggtcg cggcaacggt cccatcgggc acagcctggc
     7261 gaccacgccc tatcaccacc tgaaggcggc gcaggaaggc ggcgagtggg gtgtcgagac
     7321 ctgggattcg gtctggtcgc cgggcgggac caggcactga tccacagaat ttttgttcgg
     7381 aatccaagct tttcgctgga tcggcgggct ccgtctcaat aggcctgagc gcgaatggcc
     7441 cggccctgct tcaagctcga gaaagtctat cccgatccgg tcgccggagt cgacgaagcc
     7501 ggctgcgcgc cgctcgccgg gccggtggtc gccgccgcgg tgatcctcga tcgggagaaa
     7561 ttcccgaggg ggatcgacga ttcgaaggcg cttcctgccg cgacccgcga ggcgatctgc
     7621 gcgaagctct acaaggtggc tcggatcggg gtcggcctcg ccacggtcga ggagatcgac
     7681 gagatcaata tctactgggc gcggatgctg gccatgaccc gcgccgtcga cgcgctcggg
     7741 ctcgacccgg cgatgatcct ggtcgacggc aaccgctgcc cgaaatggga gcgcccttcc
     7801 gtgtcgatcg tcgccggcga caccaaatgc cgctcgatcg ccgccgcctc gatcgtcgcc
     7861 aaggtcaccc gcgaccggat catgctcgac tatgcgcgcg agcatcccgg ttacggctgg
     7921 gagaccaaca agggctatcc gacgccccaa cactaccgcg ccctcgattc gctcggtccc
     7981 actccgcttc accgccgaag cttcgcgccg gtgcggcagg tgatgatgaa cctggcgcac
     8041 gcggtcgaag cgcaggccgc tgattccgga gaccgctgat acgtcatcct gaacttgttt
     8101 caggatccag tttcgagccg ccccgcgggt gaaggcctgg accctgaagc aagttcaggg
     8161 tgactgggag cgctcagaac tcgccggcga tccacaacgt gtccgagtcc cacgcttcgc
     8221 ccttgcaggc ggcgggcagc ggctgaagga ggccctcgcc cctgaagtcg gtccaggcgg
     8281 tgccgccgct tattctgacc tgctcgccga cgcgaagcgg ccggtcccgg ccgccgccga
     8341 ccacgggcac gccgtcgcga tcgagcgaga gtcgtgcgtt ggagggccac aggaccaggg
     8401 tgtcgccgac catgaggcag ccgttcctca cctcgagcgt gccggtgagc gcggcctcca
     8461 tcagccgtcc gggaaacctg aactggggga agtgggtcgc cggagcggcg ctctgggcga
     8521 cccgggcaat cggccctgcg tcctggacga tgacgacaag cggatgaggc ttcagccgcg
     8581 cctcggcgac cgcccgccga tatttgtcca tctccgaaat ggagaaggtg acctggttct
     8641 tctcctcgtc gccggagctg aaggtgtagt ggatttcgag ctgccggagc agcttgccgt
     8701 aggaatcctg aagcgcgcgc aattgcggcc gggtcagccc gaccgatttg ggccagtagc
     8761 gcgggtcgct ggtgtagcgg gcgaggtggg cggcggcgtc gccggtgaac agcaccacgg
     8821 cgtgcggttc gttgtcgttc ttgaacaccc agccggcgaa ggcggggtcg gcctggacgc
     8881 gccgggcgag cgcccgcgat tcgcgctgga aatcgaggat tcccgggtgc ggatagccgg
     8941 ggtggcgggc ggagcgcgcg agcccctgca tgtggctgct ccactccgcc gtgcccggcg
     9001 ccgggggacg ggggagaggc cgcgccgacc cctgcccgtc cgtcatccgg gtcgaggaga
     9061 attcgagccg gaccggatgg cccagcaggc gctccagttc gggttcggag gcgcgggcac
     9121 gctcgacgtc ctcgccgacg gcgtagacgt tgagcatcac ctcgccggtg cgctcgtcga
     9181 ctccggtgcc catcagcccg ggaagccggg cgccgacggc cggcatcgcc gcagcgacct
     9241 tttcgagggt ttccgccagg gtcgccgggg cgccggcgag gaactggatc cgcatccgat
     9301 cccggccgcc gccgatccag cggtccgcga ccggagccgg gcccttgagc cggacgacga
     9361 ggcggtaatc gccttcgctc tcgaggtaga gtccggccag cctgtcggcg aattcgcggc
     9421 gaagcttcga gacatgcggg gccgacacct cctgcattcg aagcctactc gccgcctcgg
     9481 cctcgccgat gccgctgccg cgcgcgacct ccgcagcgtc cggatagctg gccgtctccg
     9541 agggctgcac gggatcgatt cgccgagggt cttccgcctg ggccgccgcg gcagtgagcc
     9601 agacggccag attggagagg agtgacgccg ccattcgccg aatcatggcg gaccccgcct
     9661 tgactcaggc tgaccggcgc cgccgccggc gtccgcccgc acttcggggg cggctgagtc
     9721 ctttcggcca cacccacatc atcttgagtc cgcatcggtc aggcagactc cattagtagc
     9781 ggagactcgt ttcgttcccg gcatgttaac cctattctca gcctcggtcc gtaaagcgtt
     9841 gttgttgacc gcggactccg cctgactcag gctggcggtc tggacgaacc ggggggcggt
     9901 atcgaatgac tgcacatgtg cgcttgggtt cgagggcgaa aagcgcatcg ccgccggtcc
     9961 cggacccggc gctgccgctg aaccagatcc tccaggaaga ttgcatcgcg gcgatggcgc
    10021 gcctgccggc gggcagcgtc gacatggtct tcgccgatcc gccctataac cttcagctcg
    10081 gcggcgacct gttccggccc gaaggcgggc tggtcgacgc ggtcgacgac gattgggaca
    10141 agttcgcgac tttcgccgcc tatgatgaat tcacccgcgc ctggctgaag gaagcaaggc
    10201 ggatcctgaa gccgaacggc acgctgtggg tgatcggaag ctaccacaac atctaccggg
    10261 tcggcgcagc cctccaggac gccggctact ggatcctgaa cgacatcatc tggcgcaagt
    10321 ccaacccgat gcccaatttc cggggcaccc gattcaccaa cgcccacgag accctgatct
    10381 gggcgagccg gagcgaggac agccgctata cgttcaacta tcgggcgatg aaggcgctga
    10441 acgacgagct ccagatgcgc tccgactggc tactcccgat ctgctctggg ggcgagcggg
    10501 tcaaggacgg cgggaccaag gcccatccga cccagaagcc ggaaag
//
LOCUS       DASWNU010000117         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_159710, whole genome shotgun sequence.
ACCESSION   DASWNU010000117 DASWNU010000000
VERSION     DASWNU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159710"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VGB57_02295"
     CDS             <1..414
                     /locus_tag="VGB57_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130207.1"
                     /translation="RVVRSASLARAVRKACCFVAGTLVDTEVGLRPIESIKEGERVWA
                     QDVATGEIALKAVLSAVTNLAALRGEAVTEASRWGVDGWELFAGSGPDTPQEELRVVP
                     IATLVGSDQSLLKVLSLDVGKSLWRESGEDAWHDW"
     gene            complement(791..1189)
                     /locus_tag="VGB57_02300"
     CDS             complement(791..1189)
                     /locus_tag="VGB57_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY0130208.1"
                     /translation="MAWAYAYTSPSLADPGGSGAVVAAVLWLQDTLLGTVATTVAVIC
                     VATVGLMMLSGRLDLRRGASVVVGCFILFGASSIAAGIQSVAGGGYASAESSFEVQPW
                     VPPPPPPPPPAPTAPAVPADPYAVASVPTP"
BASE COUNT          270 a          505 c          535 g          235 t
ORIGIN      
        1 agagtcgtcc ggtcagcaag cctagcacga gccgtgcgca aggcctgctg cttcgtcgcc
       61 gggacgctag tcgacaccga agtaggcctt cgtccaatcg agtccatcaa ggaaggcgag
      121 agagtttggg cgcaggatgt cgcgacgggc gagatcgcct tgaaagcggt cttgtcggcg
      181 gtgaccaatc tggcagccct tcgcggtgag gcggtcaccg aggcttctcg ttggggtgtt
      241 gatgggtggg agttgttcgc cggttcgggt cccgacaccc ctcaggagga actcagggtc
      301 gtcccaatcg ccacgctcgt cggttcggat caatccttgt taaaggtgct ttctctcgac
      361 gtgggcaagt ccttgtggcg ggagtcgggt gaagacgctt ggcatgactg gtagaccgaa
      421 ctgcgccgcc ggcacaacgg cgctgcgata atccgggaca gttactaatt tccggagctg
      481 agggcagtcc tactgcacag ctacgacccg gccggccagc tgatccggaa ctagccggac
      541 gatcggtcat ctctacgacc cggccggcaa ccgcacccgg atcacccacc ccgacggcgg
      601 ctggctggcc tatgaatatg acggcgccgg ccgcttcgtc aggctccgcg aggacagcgg
      661 cgacgtcctg gccgccttcg cctacgacgc cgccggacgc cgctccggcc tgaccagcgg
      721 cggaacggcg agcagcttcg gctacgaccc ggcccgcaaa gaccggacat gtccctccct
      781 gcgggccccg tcagggagtc gggaccgagg cgacggcata agggtccgcc gggacggcgg
      841 gtgccgtcgg cgcgggcggg ggaggcggcg gcggtggcgg tacccagggc tgcacttcga
      901 aggacgattc cgccgatgca tagccgccgc cggcgaccga ctggatcccg gccgcgatgc
      961 tcgaagcccc gaagaggatg aagcagccca cgaccacgct cgcgcctcgg cgcaggtcca
     1021 gccgccccga cagcatcatc agcccgaccg tcgcgacgca gatcaccgcc acggtggtcg
     1081 cgaccgtccc gagcaaagta tcctgaagcc acaggacggc cgcgaccacc gctcccgaac
     1141 cgccgggatc ggcgagggag ggcgacgtat atgcgtaggc ccaggccacc ttttcgcttg
     1201 cccttcgttg cgacgagtcc ctcgcttata ggcgagcgag cgctcgaacc caatcacgat
     1261 ggcggcgaag gggccctttt tcgagggcct tcggagccgg tccggaggaa aagggccggc
     1321 cgggccggag cgggcggacc gcgacgccta tcaatttcac tccgtctcgc ttattcggga
     1381 gcaaggaaag gatcgctcca tgacgaagcc agcgaccggc gacggcgagc tgttcatcca
     1441 tctggacgtc ggcggtctcg cagcgctgat gaaggcgatc gaggcggcga tgacggacgg
     1501 gcgcgggcag ctgtccctgc gcggcggcgg catggtcgtc agcag
//
LOCUS       DASWNU010000118         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_497886, whole genome shotgun sequence.
ACCESSION   DASWNU010000118 DASWNU010000000
VERSION     DASWNU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497886"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..740)
                     /locus_tag="VGB57_02305"
     CDS             complement(126..740)
                     /locus_tag="VGB57_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0130209.1"
                     /translation="MRIGCGPRFTFRDIPPGLIAMGFGPRGWGGGGGWEGGDWSAEFG
                     RGRGRGKRRMFESGELRLVLLKLIADQPRHGYDLIRAIEELTHGSYAPSPGVVYPTLT
                     MLQDMGLIQEAAADGTRKAFAVTPEGEAHLAERAEEVEALFERLTGAGEGKRRAGGRP
                     IQRAVGNLLSALWHRVTADDTDEARLHEIAAILDEAAQRIERLK"
     gene            968..2293
                     /locus_tag="VGB57_02310"
     CDS             968..2293
                     /locus_tag="VGB57_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0130210.1"
                     /translation="MADLFAGDPPPPPPTAGPPESAPLADRLRPRALDEVVGQDHLTG
                     PEGAIGRMVAAGKLASMVLWGPPGTGKTTIARLLADAVGMRFVAISAVFSGVADLKKV
                     FAEAREHVKLGRQTLLFVDEIHRFNRAQQDGFLPYVEDGTVVLVGATTENPSFELNAA
                     LLSRAQVLILNRLGSAALCKLLDRAEELVGRPLPLTREARDALVATADGDGRFLLNQA
                     ETLFSVGLPEPLDPAALSALLHRRVAVYDKDREGHYNLISALHKAIRGSDPQAALYYL
                     ARMLTAGEQPLFLLRRLIRAAVEDIGLADPQALVQCIAAKDAYDFLGSPEGELGIAQA
                     CLYLATAPKSNAVYVAQKAAWRSARETGSLMPPRNILNAPTKLMKDVGYGKGYQYDHD
                     AEGGFSGDDYWPEEMQPQTYYTPTDRGFEKRVAERLAWWDEQRKAKRDG"
     gene            2286..2639
                     /locus_tag="VGB57_02315"
     CDS             2286..2639
                     /locus_tag="VGB57_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY0130211.1"
                     /translation="MAEAIASWDEAVAFALSLPDTELSTSYGKPAVKVNGRAFLYTGR
                     EEETSFGVAIDLDTVEMLKETDPDTFWQTQHYEGWPAVLVRYGSPDPERVREIVARSH
                     AWTAAKPKPRPRKGK"
BASE COUNT          432 a          980 c          906 g          438 t
ORIGIN      
        1 ctcgtcccgg ctcggccgtc attgcgagcg aagcgaagca atccagccag ggtgcgtgtc
       61 gcaaccgcgg cgctggattg cttcgtcgct gcgctcctcg caatgacgag gcgctgcgct
      121 cctcgtcatt tcagccgttc gatcctttgc gccgcctcgt cgaggatggc ggcgatctcg
      181 tgcagtcggg cctcgtccgt gtcgtcggcg gtgacccggt gccacaaagc cgagaggaga
      241 ttgccgacgg cgcgctggat cggcctgccg ccggcccggc gcttgccttc gccggcgccg
      301 gtcagccgct cgaacaaggc ctcgacctcc tcggcgcgct cggcgagatg ggcctcgcct
      361 tcgggcgtga ccgcgaaggc cttgcgggtc ccgtccgccg ccgcttcctg gatcagcccc
      421 atgtcctgga gcatggtcag ggtcgggtag acgacgcccg ggctgggcgc gtagctgccg
      481 tgggtgagct cctcgatcgc ccggatgagg tcgtagccgt ggcggggctg gtcggcgatc
      541 agcttcagga ggacgagccg aagctcgccc gattcgaaca tccgccgttt gccgcggccg
      601 cggccgcggc cgaactcggc gctccagtcg ccgccttccc agccgccgcc gccgccccag
      661 ccgcgcggcc cgaagcccat cgcgatcagg ccgggaggaa tgtcgcggaa ggtgaaacgg
      721 ggtccacagc ctatcctcat atcctgtctc ccaatctatc tagagctagc tgagatatat
      781 cttagaccga tctgagtcaa tggattcacg tcattccggc ggaggccgga atccatgagc
      841 acggctggtg gaaaatccgg agccggcggt gttcatggat cccggccttc gccgggatga
      901 cggtcccccc gaagccagat cgacttggtg gttcaactgg gcaccatacc gctctagaaa
      961 gcccgccatg gccgatctgt tcgccggcga tccgccgcct cctccgccga ccgccgggcc
     1021 gcccgaaagc gcgccgctgg cggaccgcct gcggccgcgc gcgctcgacg aggtggtcgg
     1081 gcaggaccat ctcaccgggc cggagggtgc gatcgggcga atggtcgccg cgggcaagct
     1141 cgcctcgatg gttctgtggg ggccgcccgg caccggcaag acgaccatcg cgcgcctgct
     1201 cgccgacgcg gtcgggatgc gcttcgtcgc catctccgcg gtcttctcgg gagtggccga
     1261 tctcaagaag gtgttcgccg aagcgcgaga acatgtgaag ctgggccgcc agaccctgct
     1321 gttcgtggac gagatccacc gcttcaaccg cgcccagcag gacggcttcc tgccctatgt
     1381 cgaggacggc accgtcgtcc tcgtcggcgc caccaccgaa aacccgtcgt tcgagctcaa
     1441 cgcggccttg ctgtcgcgcg cccaggtgct gatcctgaac cggctgggca gcgccgcctt
     1501 gtgcaagctt ctcgaccgcg ccgaagagct ggtcggccgg ccgctgccgc tgacccgcga
     1561 ggcgcgggac gcgctggtcg ccaccgccga cggcgacggc cgtttccttc tcaaccaggc
     1621 cgagactctc ttttcggtcg gcctgcccga gccgctcgac cccgccgccc tctccgccct
     1681 ccttcaccgc cgggtcgccg tctacgacaa ggaccgggag ggccattaca acctcatctc
     1741 cgcgctccac aaagcgatcc gcggctccga ccctcaggcg gccctctatt atctggcgcg
     1801 gatgctgacc gcgggcgagc agccgctctt tcttctgcgc cggctgatcc gggcggccgt
     1861 cgaggatatc ggcctcgccg acccgcaggc cctcgttcag tgcatcgcgg ccaaggacgc
     1921 ttacgacttc ctcggctcgc ccgaaggcga gctgggcatc gcccaggcct gcctctacct
     1981 cgccaccgcg cccaaatcga acgccgtcta cgtcgcccag aaggcggcat ggcgctcggc
     2041 gcgggagacc ggctcgctga tgccgcccag gaacatcctc aacgcgccca cgaagctgat
     2101 gaaggacgtc ggctacggca agggctacca atatgatcac gacgccgagg gcggcttctc
     2161 cggcgacgat tactggcccg aggagatgca gccgcagacc tactacacgc ccaccgatcg
     2221 aggcttcgag aagcgagtcg cggagcggct cgcctggtgg gacgagcagc gcaaggcgaa
     2281 gcgcgatggc tgaagcgatc gcgagctggg acgaggccgt cgccttcgct ttgtcgctcc
     2341 cggatacgga gctctccacc agctacggca agcccgcggt caaggtgaac ggccgcgcct
     2401 tcctctacac cggccgggag gaggagacgt cgttcggagt cgccatcgat ctcgatacgg
     2461 tggagatgct gaaggaaacc gatcccgaca ctttctggca gacgcagcat tacgaaggct
     2521 ggccggcggt gctggtccgc tacggcagcc cggatcccga gcgagtccgg gagatcgtcg
     2581 cacgttcgca cgcctggacc gccgccaagc ccaagcccag gccgaggaaa ggaaagtgat
     2641 catgcaagct atcgtcatgc cagcgaaggc tggcatcccg gcgggtgaaa ggcgcctgcg
     2701 ttcttcaccc cccggggccc cagccttcgc tggggcgacg aaggcgctgc tcgccg
//
LOCUS       DASWNU010000119         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_497922, whole genome shotgun sequence.
ACCESSION   DASWNU010000119 DASWNU010000000
VERSION     DASWNU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497922"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1990
                     /locus_tag="VGB57_02320"
     CDS             <1..>1990
                     /locus_tag="VGB57_02320"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0130212.1"
                     /translation="ERLGTPVVLSSAAVDPSGTIDLVALGDGRMAAVWTDAAGDLSGQ
                     ILQSLVGTFEGESLSGGPGDDTIFGSPGNDILRGNDGDDALHAYEGSDDLFGGDGDDA
                     LFGGDGDDILRGGPGVDSFDGGDHTTLFDWYRGYGDRVSFFEEEATQGVVADLRTGII
                     SNDGFGNVETMANIESLGADTAYVDTLYGNDSSNFLFAGLGDHLHGFGGNDILLVYAA
                     AATVDGGDGLDTLRLVSYFAGYRPDGDGDGLAETGSFPTSGYTVDLLAGTFRDGDGNT
                     GIVTGIENLAGSSFADALRGNGGNNTLRGEGGNDIFQLQDGGVDLVRGDSGNDIFLFL
                     GTLTAEDRVDGQDQDGGVDTLVLQGDYGGGLTLTANVNLIDRIAFLAGSNTHFGEPGT
                     NRHDYVLTTHDSNFGSGVRARINGSALLAGEDLTFDGSAETDAGFDIYGGRGVDTLTG
                     GLRNDVFFFDEERLAPGDTVDGGGGYDALFLRGNYSVDFDDPGYGGLFTSIENLTLTS
                     VTDSRYGRGGTGFDYDLTLSDSLVDPGQVLTVSGAVLMAGETMVLDASRETDGLLRLF
                     GGKASDTLKGGGQNDLIHGNLGADSLTGNGGADAFRYQNVDESSSASMDRILDFTPGT
                     DKVELDRIDANALVAGNQAFSWIGSSAFTGSAGQLRAYE"
BASE COUNT          338 a          670 c          666 g          316 t
ORIGIN      
        1 cgaaaggctg ggaacgcccg tcgtcctgag cagcgccgcc gtcgatccct ccggaacgat
       61 cgacctcgtc gccctgggcg acgggcggat ggcggccgtc tggaccgatg cggcgggcga
      121 cctctccggg cagattctcc agtcgctggt cggcaccttc gagggcgaat ctctttccgg
      181 cggacccggc gacgacacga tcttcggcag ccccggcaac gatattctcc gcggcaacga
      241 cggcgacgat gcccttcatg cctatgaagg cagcgacgac ctcttcggcg gcgacggcga
      301 cgacgccctg ttcggcggcg acggcgacga tatcctgcgc ggcggccccg gcgtcgattc
      361 gttcgacggc ggcgatcaca ccaccctctt cgactggtac aggggctatg gcgaccgggt
      421 cagcttcttc gaggaggaag cgacgcaggg cgtcgtcgcc gacctgagaa cagggattat
      481 ctccaacgac ggcttcggca atgtcgagac gatggcgaac atcgaatcgc tcggggccga
      541 caccgcctat gtcgacacgc tctacggcaa cgattcgagc aacttcctct tcgccgggct
      601 gggcgaccat ctccacggct tcggcggcaa cgatatcctg ctggtctacg ccgcggcggc
      661 gacggtcgac ggcggcgacg ggttggacac gctccggctc gtctcctact tcgccggcta
      721 ccggcccgac ggcgacggcg acggcttagc cgaaaccggg tctttcccga ccagcggcta
      781 taccgtcgat ctgctggccg gcaccttccg cgacggcgac ggcaataccg gaatcgtcac
      841 cggcatcgag aacctcgccg gcagctcgtt cgccgacgcg ctgcgcggca atggcggcaa
      901 caacacgctg cgcggcgagg gcggaaacga tatcttccag ctccaggacg ggggcgtgga
      961 tctagtccgg ggggacagcg gcaacgacat tttcctcttc ctgggaacgc tcaccgcgga
     1021 ggacagggtc gacggccagg accaggacgg gggcgtcgac acgctcgtcc ttcagggcga
     1081 ctatgggggc ggcctgacgc tcaccgccaa cgtcaacctt atcgacagga tcgccttcct
     1141 cgcgggctcg aacacccatt tcggagagcc gggcaccaac cgccacgatt atgtgctgac
     1201 cacccacgat tccaacttcg ggtcaggcgt gcgggcgcgg atcaacggct cggcgcttct
     1261 ggcgggcgag gatctcacct tcgacggctc cgccgaaacc gacgccggct tcgacatcta
     1321 cggcggcagg ggggtcgata cgctgaccgg cggcctccgc aacgacgtct tcttcttcga
     1381 cgaggagcgg ctcgcgccgg gagacaccgt cgacggcggc ggcggctatg acgcgttgtt
     1441 cctgaggggg aattactcgg tagacttcga cgacccgggc tatggcgggt tgttcacgag
     1501 catcgagaac ctgaccctga cctcggtcac cgactcgcgc tatgggcgcg gcggcaccgg
     1561 gttcgactac gatttgaccc tctccgactc actcgtggat ccggggcagg tgctgaccgt
     1621 gtcgggcgcg gtgctgatgg cgggcgagac gatggtgctc gacgcgagcc gggagacgga
     1681 cggcctcttg cggctgttcg gcggcaaggc ctccgacacc ttgaagggcg gcggccagaa
     1741 cgacctcatc cacggcaacc tcggcgccga cagcctcacc ggcaatggcg gggcggacgc
     1801 attccgctac cagaacgtcg atgaatcgag ctcggcttcg atggaccgga tcctcgactt
     1861 caccccgggg acggacaagg tcgagctcga ccggatcgac gccaacgcgc tcgtcgcggg
     1921 caaccaggcg ttcagctgga tcggctcgag cgccttcacc ggctcggcgg ggcagcttcg
     1981 agcctatgag
//
LOCUS       DASWNU010000120         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_420326, whole genome shotgun sequence.
ACCESSION   DASWNU010000120 DASWNU010000000
VERSION     DASWNU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420326"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1024)
                     /gene="ligD"
                     /locus_tag="VGB57_02325"
     CDS             complement(146..1024)
                     /gene="ligD"
                     /locus_tag="VGB57_02325"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0130213.1"
                     /translation="MSEEKIVAGVRLTSPDKVLWHEQGVTKSELAEYYLAVAEVMLPH
                     VARRPVTMVRCPAGAEKKCFYQRHAASGVLPELKEVPIPGFDEPYLYIEDAAGLVAMV
                     QMGTLEIHPWGVTVERPDRPDRIVFDLDPDEGLGFADVVRATLEVRDRLSSLGLESFV
                     KTTGGKGLHIVLPIEPVSEWRAAKAFAKSFSEAMAADSPERYLTKISKAERAGRIFID
                     YLRNDPTSTAVGPYSTRSRPGAPVATPLEWEEVTPGLDPKKFNVETVPKRIRSIRRDP
                     WADIGRVRQHLPDVPA"
     gene            complement(1021..>1573)
                     /locus_tag="VGB57_02330"
     CDS             complement(1021..>1573)
                     /locus_tag="VGB57_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0130214.1"
                     /translation="REVAQGWLDMAGGALDGVVAKRSDGPYESGERAMLKIKTMRTAD
                     CVVGGFRYGTGSRIVGSLLLGLHNEEGKLDHVGFTSSFGNEDKAALTKRLEALVGPPG
                     FTGEAPGGPSRWATERSGDWQPLRPELVLEVRYDHVTGKRFRHGTKFLRWRPDKAPEQ
                     CRFDQLRHEAPPAALVAELEAAQ"
BASE COUNT          247 a          540 c          527 g          259 t
ORIGIN      
        1 tgcggtcgtc gtccaccacg tagacggtac gggaactcaa acggcgcctt tcgaaagccc
       61 cgaatgaggg gccccagcac catgatttcg gcaatggccg gcggcaagcc ctttccgctc
      121 gcattccgga cctagttcgg atcgatcacg ccggcacgtc gggcagatgc tggcgcaccc
      181 ggccgatgtc ggcccaggga tcgcggcgga tcgagcggat ccgcttcggc accgtctcca
      241 cgttgaactt tttcgggtcg agcccaggcg tcacttcctc ccactccagc ggagtcgcga
      301 cgggggcgcc cggccgcgag cgggtggaat agggcccgac ggcggtggag gtcggatcgt
      361 tgcgaagata gtcgatgaag atccgcccgg cccgctccgc cttggagatt ttggtgaggt
      421 agcgttcggg agaatcggcc gccatcgctt cggaaaagct tttggcgaac gccttggccg
      481 cgcgccattc ggagaccggc tcgatcggca gcacgatgtg aaggcccttg ccgccggtcg
      541 tcttgacgaa gctttcgagc ccgagcgagg aaaggcgatc gcggacctcc agggtcgcgc
      601 ggaccacgtc ggcaaagccg aggccctcgt ccggatcgag atcgaagacg atcctgtccg
      661 gccggtcggg ccgctcgacg gtgacgcccc agggatggat ttcgagggtg cccatctgca
      721 ccatcgcgac gagcccggcc gcgtcctcga tatagagata gggctcgtcg aagcccggga
      781 tcgggacttc cttgagctcg ggcagcacgc ccgaagcggc atgacgctgg tagaagcatt
      841 tcttctccgc acccgccggg cagcgcacca tcgtcaccgg gcgacgcgcc acgtgcggca
      901 gcataacttc ggcgacggcg agataatatt cggcgagctc gctcttggtg acgccctgct
      961 cgtgccacag caccttgtcc gggctggtca ggcggacgcc cgcgacgatc ttttcctcgc
     1021 tcactgcgcc gcctccaact ctgcgacgag cgcagcgggg ggcgcctcgt gccgcaattg
     1081 atcgaaccgg cactgctcgg gcgccttgtc cggccgccag cgcaggaatt tggtgccgtg
     1141 gcggaagcgc ttgccggtga catggtcgta acgcacctcg aggaccagct cgggccggag
     1201 cggctgccag tcgcccgagc gctcggtcgc ccagcggctg ggcccgccgg gggcctcgcc
     1261 ggtgaagccc ggcggcccta ccagcgcctc aagccgcttc gtaagcgctg ccttgtcctc
     1321 gttgccgaac gaggaggtga agccgacatg gtcgagcttg ccctcctcgt tgtggagacc
     1381 gagcaggagc gagccgacga tgcggcttcc cgtaccgtag cggaagccgc cgacgacgca
     1441 gtccgcggtg cgcatcgtct tgatcttgag cattgcccgc tcgccgctct cgtacggccc
     1501 gtcgctccgc ttcgcgacga cgccgtcgag cgcaccgccc gccatgtcga gccagccctg
     1561 cgccacctcc cgg
//
LOCUS       DASWNU010000121         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_341772, whole genome shotgun sequence.
ACCESSION   DASWNU010000121 DASWNU010000000
VERSION     DASWNU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341772"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..604)
                     /locus_tag="VGB57_02335"
     CDS             complement(107..604)
                     /locus_tag="VGB57_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEY0130215.1"
                     /translation="MTDSTPRFALPLILPGQAQKELFHNEAIALADILLHAAAEGAPQ
                     ADPPAGPAPGQCWIVAAGASGEWVGQDHALATWTESGWRFTRPIEGTAVWNKAAGTWS
                     IWAGTSWSSGEVSATKLMIEGQQVVGSRQSAVPSPSGGTTIDAEARSAVEGLIVALRS
                     HGLID"
     gene            complement(601..>1775)
                     /locus_tag="VGB57_02340"
     CDS             complement(601..>1775)
                     /locus_tag="VGB57_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130216.1"
                     /translation="LDAESAARSIAVLRLGWEHLALRPGMRVRMEGEGGTWTVRRWQL
                     GPAIVDLELARVPDPQTSSIAANPGRSLAAPDLVHGPTTLCVFEAPLPGYPDAARLFA
                     AAAGASAGWRRAALLVSTDGGINWEDGGHTAEPAILGPVLTTLPAAPAALFDRRSSVE
                     VELLNDGMWLESRPDAALGGGANLALLGSELVQFGRAEQTGERRFRLSRLLRGRLGTE
                     GAVSGHAPGEAFVRIDPQTLTMLPMPIAAIGATVLVTAAGLGDPIEGVRAERPFAGEA
                     LRPLMPCHLAAATDAAGDLLMTWARRSRAGFEWANGRDVPLGEEQEKYLVSVAAAGGA
                     RSFEVTEQRLLYPSAEQAADAIAPPLIIAVAQVGTYATSRPAVLVFGSPSNEEQSS"
BASE COUNT          277 a          624 c          605 g          269 t
ORIGIN      
        1 aaatgttgct gaaaagccgc agttcgcggt gagacgaacg gcaggttgtc agccgaagtt
       61 cgaacggttc cgcacctccg gggttgcatc ctccggggcc gaaaagtcaa tcgatgagac
      121 catgtgacct taatgccaca atgagtccct cgacggctga gcgcgcctcg gcatcgattg
      181 tcgttccccc tgaaggactt ggcaccgcgg attgccgcga tccgacgacc tgctgaccct
      241 ctatcatcag cttggtggcc gaaacttctc ccgaggacca gcttgttccg gcccagatcg
      301 accaggttcc cgcggccttg ttccaaaccg ccgtcccctc gatcggccgg gtgaagcgcc
      361 acccgctttc ggtccaggtg gcgagagcgt ggtcctgccc gacccactcc ccggaggccc
      421 cggcggcgac gatccagcac tggcccggcg ccggccccgc tggtgggtct gcctgcggag
      481 ccccttccgc agccgcatgc aggagaatgt ccgccagggc gatcgcctcg ttgtggaaga
      541 gctccttctg ggcctggccg ggaaggatga gcggaagcgc gaagcgcggc gtggagtcgg
      601 tcatgaactc tgctcctcat ttgagggact gccgaaaacc aggactgccg gtcgcgacgt
      661 cgcgtaggtg ccgacctgtg cgaccgcgat gatgagcggc ggcgcgatcg cgtcggcagc
      721 ctgttcggcg gagggataga ggagccgctg ctcggtcact tcgaaggacc gggcgccgcc
      781 ggcggccgcg acggagacca gatacttctc ctgttcctcg ccgagcggca cgtcgcgccc
      841 gttcgcccac tcgaaccccg ctcggcttct ccgcgcccag gtcatcaaga ggtcccctgc
      901 cgcatcggtc gcagccgcga gatggcaggg catgagcggg cgcagcgcct ccccggcgaa
      961 aggccgctcc gcgcggacgc cctcgatcgg gtctcccagg ccggccgcgg tgaccaggac
     1021 cgtcgccccg atcgccgcta tcggcatcgg cagcatcgtc agggtctggg ggtcgatccg
     1081 gacgaacgcc tcgcctggcg cgtggcccga aacggcccct tcggtcccca gcctcccgcg
     1141 gagcaggcgg ctcagccgga agcggcgctc tcctgtctgt tcggcccggc cgaactggac
     1201 gagctccgat ccgagcaagg cgagattggc tccgcccccg agcgccgcgt ccgggcggct
     1261 ttcgagccac atgccgtcgt tgagaagctc gacctcgacg ctggagcggc gatcaaacaa
     1321 ggcggccggc gcggccggaa gcgtggtgag gaccggaccg aggattgcgg gctctgcggt
     1381 atgcccgccg tcctcccagt ttatgccgcc gtcagtgctg acaagcaacg ccgcccgccg
     1441 ccaccccgcc gatgcgcccg cggcggcggc aaagaggcgg gccgcgtcgg gatatccggg
     1501 caaaggagct tcgaacacgc acagggtggt agggccgtgg accagatcgg gtgcggcaag
     1561 cgagcgtccc ggattggccg cgatcgaact cgtctgcgga tccgggacgc gggcgagctc
     1621 gaggtcgacg atggcgggcc ccagctgcca gcggcgcacg gtccacgttc cgccctcgcc
     1681 ttccatcctg acacgcattc cgggccggag cgccagatgc tcccagccga gccgcaggac
     1741 ggcgatgctc cgggctgccg attctgcgtc gagcc
//
LOCUS       DASWNU010000122         4130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_498025, whole genome shotgun sequence.
ACCESSION   DASWNU010000122 DASWNU010000000
VERSION     DASWNU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498025"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..346
                     /locus_tag="VGB57_02345"
     CDS             146..346
                     /locus_tag="VGB57_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130217.1"
                     /translation="MTEPIRERETTVVTTDGGGGGGTIIAVVLLIAIIALLFYLFGGQ
                     LLGGADSTTDVKVDVTAPAKSS"
     gene            415..735
                     /locus_tag="VGB57_02350"
     CDS             415..735
                     /locus_tag="VGB57_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130218.1"
                     /translation="MTDPDREREIPRETVRDTERTTIIQTDGGRDRGGGGVILAIVLI
                     LALLALLYFVFSGSFNRAADEVGVNVNVEAPNMPDVSLPDKIEVKVPDEIDVTTDGGG
                     NKAQ"
     gene            complement(1015..1215)
                     /locus_tag="VGB57_02355"
     CDS             complement(1015..1215)
                     /locus_tag="VGB57_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130219.1"
                     /translation="MDDDKLNGRQGGGDSGGGAYPNPHSGKEGGKDGFTGSGGQTEKP
                     YHGHGQLGEEETGDNPNAPAED"
     gene            complement(1267..1971)
                     /locus_tag="VGB57_02360"
     CDS             complement(1267..1971)
                     /locus_tag="VGB57_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0130220.1"
                     /translation="MSHQRTVYVVDNDLETRRSLTINLASIGAEAWPFGGGGEFLEIL
                     GHLMPGCILLDMDAEDGLEVLAALVRRETGWPILAMSATPKVEIAVQAMKLGALDFLG
                     KPVEAAKLAAALLPAWNALEASLEQGEAKRIAQDRLARLTARELDISLSLFGGRSNKT
                     VAHELGISVRTVEMHRAHIMAKLGVKSIAEAAVMATHAGLAIAQRRPVAPVLQPIGGG
                     RSTSPFEGRRLARTAY"
     gene            2336..2845
                     /locus_tag="VGB57_02365"
     CDS             2336..2845
                     /locus_tag="VGB57_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130221.1"
                     /translation="MEVTQIFSGNALGALDEEGRVRLPPFILREMARHEGGGRVLLGV
                     HESDPCLTGYGSALRPRLEAELERRRLRDEALGKTADEHHARARRTFGLVEEASYDES
                     GRLTLPPLARRRGGLGRHILFIGAGSHFEIWDPSLAMAEGDEELRYVAEYRLGDGTAA
                     TFEEEEDEG"
     gene            2842..3279
                     /locus_tag="VGB57_02370"
     CDS             2842..3279
                     /locus_tag="VGB57_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130222.1"
                     /translation="MSLLCALGGHEAGAGEVYNGGYYFARCRRCERDMVRSGAAWQMV
                     PAGHRVVWRSGRHQHSVEPSFAGVLPVLHREANLPAVRPPFASWSRDIVRLRRPPEVA
                     AAASASALIEKEEPHYPRLLVIAALVGAGLQLIFSLGSGRSLG"
     gene            3435..4049
                     /gene="pcm"
                     /locus_tag="VGB57_02375"
     CDS             3435..4049
                     /gene="pcm"
                     /locus_tag="VGB57_02375"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY0130223.1"
                     /translation="MKPMGEQHLALLRRHMVEIVDLHFDLASEELGKDEVDPRLRAAL
                     LRVPRHLFVPHQLAVAAYQDTPLPIGFDKTISQPFIGALMLDLLDLSPGQRVLEVGTG
                     LGWQAAVMAALGAFVWSVEIVEEFAEGARLQLAAEDHDVEIRVGDGSRGWPEHAPFDR
                     ILVTAAASAPPQALGEQLAPGGRMVIPLGGKAAAAVTRIRSNGA"
BASE COUNT          661 a         1377 c         1416 g          676 t
ORIGIN      
        1 ggggcggtcg aggcttttat ccgtaccggc ccctggcccg gccgcggatc cgccgggacg
       61 gtcgcgaagc ttgagctgcg acgagagtga ggaacccccc tatcggcgca gcgttaaggc
      121 tccgaaacaa agcggagaca ccacaatgac ggagccaatt cgcgagcgcg aaaccacagt
      181 ggtcaccacc gatggcggcg gcggcggcgg gacgatcatc gccgtcgtcc tcctgatcgc
      241 gatcatcgcc ctcctcttct acctgttcgg cggccagctt ctcggcggcg ccgacagcac
      301 gaccgacgtc aaggtcgacg tgaccgcgcc ggcgaaaagc agctgatcgc ccttcgatcg
      361 gcaaattgcg gaaggcgcgg tcgggccttc cgccagtaaa ccatggagag tgtaatgacc
      421 gaccccgatc gcgagcgcga gatcccgcgc gaaacggtgc gtgacaccga gcggacgacc
      481 atcatccaga ccgacggcgg ccgcgatcgc ggcggcgggg gcgtgatcct cgccatcgtc
      541 ctgatcctgg cgcttctcgc cttgctctat ttcgtcttca gcggaagctt caaccgtgcc
      601 gcggacgagg tgggcgtcaa cgtcaacgtc gaggctccga acatgccgga cgtcagcctt
      661 ccggacaaga tcgaggtgaa ggtgcccgac gagatcgacg tcaccaccga cggcggcggc
      721 aacaaggcgc aatgaggatg tggcgggtga atggcggctg acagacccgt tcacagattc
      781 ctaaaccgaa tcaaggcata agctcggggt cggtccgcgg atcgacccct cgagtcgcgt
      841 aacgagggag gggccggcgg tcgaaaggcc gccggctcct acgttgcggg tatccgtcat
      901 cccggcgaaa gccgggacct caggacaaga agtctctgcg ggtcctcgcc gaggtcccgg
      961 ctttcgccgg gatgacgtgt gtctggccgc gccggtcttt gccgcgaccg aaagctagtc
     1021 ttccgcgggc gcgttgggat tgtcgccggt ctcttcctcg cccagctggc catggccgtg
     1081 atagggcttt tcggtctggc cgccgcttcc ggtgaagccg tccttgccgc cttccttgcc
     1141 gctgtgcggg ttgggatagg cgccgccgcc ggaatcgccg ccgccctgcc gtccgttgag
     1201 cttgtcgtcg tccatggcgc gctcctttcg cggctgaatg agccgcggcg cgcgaggttc
     1261 cgaagctcaa taggccgtgc gcgccagccg ccggccctcg aaaggcgaag tcgagcggcc
     1321 tccccctatc ggctggagga ccggggcgac cggccggcgc tgggcgatgg cgaggccggc
     1381 atgggtggcc atcaccgccg cctcggcaat gctcttcacg ccgagcttgg ccatgatgtg
     1441 cgcccggtgc atttcgacag tccggacgct gatcccgagc tcgtgggcga ccgttttgtt
     1501 ggagcggccg ccgaacaggg aaagcgagat gtcgagctcg cgcgccgtca gccgcgccag
     1561 ccggtcctgg gcgatacgct tcgcctcccc ctgctcgagc gaggcttcga gggcgttcca
     1621 ggcgggaagc aacgcggcgg cgagcttcgc cgcctcgacc ggtttcccga ggaaatccag
     1681 cgctcccagc ttcatcgcct ggacggcgat ttcgaccttg ggagtcgcgc tcatggcgag
     1741 gatcggccag ccggtctccc gccgaaccag cgcggccagg acctcgagcc cgtcctccgc
     1801 atccatgtcg agcaggatgc agcccggcat caaatgcccg agtatctcga gaaattcgcc
     1861 cccgcccccg aacggccagg cttcggcgcc gatcgacgcc agattgatcg tcagcgacct
     1921 cctcgtctcg aggtcgttgt ccaccacata cacggtgcgt tggtgcgaca taagcccgct
     1981 cccccaaagg cccaaaaaaa cacggccacg cacagtcaac tctctgagtc gactggttaa
     2041 tagacaacct aggagaatta cggtgaaccg tagatgaccg gggtcggcca agtatctggc
     2101 attgtggacc tttcttcatg agggggacaa acagaagtag gtccgaagtg acataatgta
     2161 ggttagatcc tcgaaggtgt tgcgaggccg gctcaccgta gagtccatgc cgaatcaccg
     2221 tatcattacc gatgtacaag tccgatctga gccccgcttc cgttaaccat taagcttcct
     2281 cccgtagcgg cgatgcgccg gtacgggttg ggcgttgcgg ggggcgaacg ggaccgtgga
     2341 agtcacgcag atcttcagtg gaaacgcgct cggcgcgctc gacgaagagg gccgagtccg
     2401 tctcccgcct ttcatcctgc gcgagatggc gcggcacgag ggcggcggcc gggtcctgct
     2461 cggggtccat gaaagcgacc cctgcctgac cggctacggc tcggcgctgc ggccgcggct
     2521 ggaggccgag ctcgagcggc ggcgcctgcg cgacgaagcg ttggggaaga cggcggatga
     2581 gcatcatgcc cgcgcgcggc gcaccttcgg cctcgtcgag gaagcgagtt acgacgaatc
     2641 cggccggctg accctgccgc cgctggcgcg gcgccgcggc ggtctcggcc gccatatctt
     2701 gttcatcggc gccggcagcc atttcgagat ctgggatccg tcgctggcca tggccgaggg
     2761 cgacgaggag ctgcgctacg tcgccgaata ccggctcggc gacggcactg ccgcgacatt
     2821 cgaagaggag gaggacgagg gatgagcctg ttatgcgcac tgggcggcca cgaggcgggg
     2881 gccggggaag tctataatgg cggttattat ttcgcccgct gccggcgctg cgagcgcgac
     2941 atggtccgga gcggcgccgc ctggcagatg gtgccggccg gccaccgggt cgtctggcgg
     3001 agcggccggc accagcattc ggtcgagccg agcttcgccg gggtcctgcc ggtcctgcac
     3061 cgcgaggcca atctgccggc ggttcgcccg cccttcgcct cgtggagccg cgacatcgtc
     3121 cggctgcggc gccctcccga ggtcgccgcg gcggcttcgg cctcggctct gatcgagaag
     3181 gaggagccgc attatccgcg gctgctggtc atcgccgcgt tggtcggagc ggggcttcag
     3241 ctgatcttca gcctcggctc ggggcgcagc ctggggtgaa ccgtcattcc ggcgaaagcc
     3301 gggacctcag ggagataaag cccggccagg cggcccgggg tgccggcttt cgccgggatg
     3361 acgttcggtc ggccggagcc ttgcgcggcg ccgtgcattc tgcgccttgt cggaagcaag
     3421 gaggcgccac tcgaatgaag ccgatgggcg agcagcatct ggccctcttg aggcgccaca
     3481 tggtggagat cgtcgatctc catttcgacc tggcgagcga ggagctcggc aaggacgagg
     3541 tcgacccgcg gcttcgcgcc gccctgctgc gcgtgccccg tcatctgttc gtgccccacc
     3601 agctcgccgt cgccgcctat caggatacgc cgcttccgat cggcttcgac aagaccatat
     3661 cccagccctt catcggcgcc ctcatgctcg acctgctcga cctttcgcca ggccagcgcg
     3721 tgctggaggt gggaaccggg ctcggctggc aggccgcggt gatggcggcg ctcggcgcct
     3781 tcgtctggag cgtggagatc gtcgaggaat tcgccgaagg ggcccggctc cagctcgccg
     3841 cggaagacca tgacgtcgag atcagggtcg gcgacggctc gcgcggctgg cccgagcatg
     3901 ccccgttcga ccggatcctc gtcaccgccg ccgcctccgc cccgccccag gccctcggcg
     3961 agcagctcgc gccgggcggc cggatggtga tcccgctggg cgggaaggct gcagcggcgg
     4021 tgacgaggat ccgatcgaac ggggcgtgat ccggccagcc gcgcgatccg tccccgaccc
     4081 ggatgtcgac gtcatgatcc tgttcggcca ggcggagacg cgcagcctgc
//
LOCUS       DASWNU010000123         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_212426, whole genome shotgun sequence.
ACCESSION   DASWNU010000123 DASWNU010000000
VERSION     DASWNU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212426"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGB57_02380"
     CDS             <1..383
                     /locus_tag="VGB57_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130224.1"
                     /translation="SDPEEVDRRIGEEEDPQVWCEDGIWWTCFPPPDGFDGEEEGEPS
                     DGDYQRTLTEDEEAVMQARNRAADAAELTRCCNVRDKYFGLSPRGCSETFFPQGSRNY
                     ETSEPFETGDGEELDPLYLGSSPG"
     gene            457..1488
                     /locus_tag="VGB57_02385"
     CDS             457..1488
                     /locus_tag="VGB57_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase"
                     /protein_id="HEY0130225.1"
                     /translation="MKHIPRRQGPLRIFDEDKDRLVILFLAKHALSGGVPDAQDGTHA
                     VYHHELLETLKTAGFFVSAANSYDHIWRRPDADFVVPLLNRGGFLNSEMLAPLILTRH
                     GLPFLGAAPMLRGLSDDKHLMKTAARLRGVPVTDWAIVRRGQGKVEPPPFRWKRLVVK
                     PNASSASWGVKILDDWTDARAHAEHILDSGHDVILEPYAPLIDIAVPVVGADGPWLLP
                     PMGYFPDVPGMPRSYEEKRGLVETGDEDPLRIVDDPALAGRLNEAVRALLPELWPFDY
                     GRFEFRYDPATDDIRFMEVNLSCNLWSKKTISRSARTLGVSHARLVETILAHSLLRQG
                     VVKDVVRLY"
     gene            complement(1661..>1825)
                     /locus_tag="VGB57_02390"
     CDS             complement(1661..>1825)
                     /locus_tag="VGB57_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0130226.1"
                     /translation="GDLFAAGFLTGRAQGRSNADCLRMGAIAAAEVISHYGARPEADL
                     KALVAEKLGA"
BASE COUNT          312 a          649 c          571 g          293 t
ORIGIN      
        1 cctccgatcc ggaagaggtg gaccggcgga tcggagagga agaggacccg caggtctggt
       61 gcgaggacgg aatctggtgg acctgcttcc ctccgcccga cggcttcgac ggcgaagagg
      121 aaggcgagcc gagcgacggc gactatcagc gcaccctcac cgaagacgag gaagcggtca
      181 tgcaggcgcg caaccgggcc gccgacgcgg ccgagctcac ccgctgctgc aacgtgcgcg
      241 acaaatattt cgggctctcg ccgcgcggct gctcggaaac attctttccc cagggaagcc
      301 gaaactacga aacttccgaa cctttcgaaa cgggcgatgg ggaggaactc gaccccctct
      361 atctcggatc gagtccggga tgacgataat cgcccaaccc acatccaaac cgcatccgcc
      421 cggcatcctt gctgatgaac caccgggagc agtagcgtga aacatatccc ccgccggcaa
      481 gggccgcttc gaatcttcga cgaggacaag gaccgtctcg tcatcctctt cctcgccaag
      541 cacgcgctgt ccggcggagt gccggacgcg caggacggca cccatgccgt ctatcatcac
      601 gagttgctcg agaccctgaa gacggccggc ttcttcgtct cggccgccaa cagctacgat
      661 catatctggc ggcggccgga cgcggatttc gtcgttccgc ttctcaaccg cggcggcttc
      721 ctcaattcgg agatgctcgc gccgttgatc ctgacccgcc acggcctccc cttcctcggc
      781 gcggcgccga tgcttcgcgg cctttcggac gacaagcacc tgatgaagac cgccgcccgg
      841 ctgcgcggcg ttccggtcac cgactgggcg atcgtccggc gcggccaggg caaggtcgag
      901 ccgccaccct tccgctggaa gcggctggtg gtgaagccca atgcgagctc cgcctcctgg
      961 ggggtgaaga tcctcgacga ctggaccgac gcccgggccc atgccgagca tatcctcgac
     1021 tccggtcacg acgtcatcct ggagccctac gcgccgctga tcgacatcgc cgtcccggtc
     1081 gtcggagccg acggcccctg gctgcttccg ccgatgggct atttcccgga cgtgcccgga
     1141 atgccgcgaa gttacgagga gaagcgcggc ctggtcgaga ctggggacga ggacccgctc
     1201 cggatcgtcg acgatcccgc cctcgccggc cgcctgaacg aggcggtgcg cgccctgctc
     1261 ccggagctgt ggccgttcga ttacggccgc ttcgagttcc gctacgatcc cgccaccgac
     1321 gacatccgct tcatggaggt caacctctcc tgcaacctgt ggtcgaagaa gaccatctcg
     1381 cgctccgccc ggacgctcgg cgtcagccac gcccgcctcg tcgagaccat cctcgcccac
     1441 agcctgctcc gccagggcgt ggtgaaggac gtggtgaggc tctactgatg gggggaatat
     1501 cgctcccatt ttccgccgaa ggtcgggacc ccaaattcct ccccgcgatt tcgcggggag
     1561 ggggaccgcg cgaagcgcgg tggaggggtt cttcggactc cgtaaggccc ctccaccacc
     1621 cttcgggtgg tccccctccc ctgcaaatgc aggggaggat ttaagcgccg agcttttcgg
     1681 cgaccagcgc cttcagatcc gcttccggcc gggcgccgta atgcgagatc acctcggccg
     1741 cggcgatcgc gcccatccgg aggcaatccg cgttcgagcg gccctgggcg cgccccgtca
     1801 ggaaaccggc ggcgaacagg tctcc
//
LOCUS       DASWNU010000124         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_108779, whole genome shotgun sequence.
ACCESSION   DASWNU010000124 DASWNU010000000
VERSION     DASWNU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108779"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..828
                     /locus_tag="VGB57_02395"
     CDS             223..828
                     /locus_tag="VGB57_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130227.1"
                     /translation="MLKSIFAVISLAAASFLPNGAAARGEATQVAHTSWLGIRIVQDG
                     KPVRLVRTDLRTTEARLAPRPFRMVFPKRRADVAYQLCAWTGKSIFRDLRPRVVPGNP
                     NAGGEHPCFAPGTGMADTEAGSGMLMLSNSGNNYLIDLRLGPDPERPSVYYSALWTRA
                     RGQVPIAAARRPVYIVAFHDSDGDGFMANSEYDHLVLRFAR"
     gene            complement(964..2139)
                     /gene="purT"
                     /locus_tag="VGB57_02400"
     CDS             complement(964..2139)
                     /gene="purT"
                     /locus_tag="VGB57_02400"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEY0130228.1"
                     /translation="MSFTARVLLLGSGELGKEFAISAKRLGAWVVACDSYAGAPAMQV
                     ADEAEVFSMLDGERLREAIERHRPDFVVPEIEAIRTEVLAELESAGIHVVPTARATTM
                     TMNRDAIRELAAGPLGLRTSLYRFAESFEEVEAAAAHTGFPCVVKPVMSSSGKGQLTV
                     EAADGLRSAWDYAVANMRGDRARVIVEQFVRFDYEITLLTVRTREGVLFCPPIGHRQE
                     RGDYRESWQPTPMSPRALAEARDMARKVVDDLGGFGLFGVEFFIAGDEVIFSELSPRP
                     HDTGMVTLISQNLSEFDLHARAILGLPIPHIGLTAPAAASAVILADRESESFRFEGVA
                     EALALGSEGAEVDVRIFGKPVTRPYRRMGVALATGATAEEARALATRAAAEVRIVYD"
     gene            complement(2136..>3617)
                     /locus_tag="VGB57_02405"
     CDS             complement(2136..>3617)
                     /locus_tag="VGB57_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130229.1"
                     /translation="AVGLEIKGAKSLAGTYDKPLARHRPGARLRVRYRIVSAYAEDPG
                     FDFEQPRPLIRPDWFFVHGEALFADPGGADDRPAKFRWGKLPKGWTVASDLDHLKGQP
                     TTLANLINSVAIGGAKLRVVRRDLQGAELRVALLGDWKFTPDQLADTVTPIVAAEDSF
                     WGDRSGPFLIAMAPLGDIPSGLSYVGAGRTDAFSIASTSAFELRQAVRFLGHEYMHSW
                     VPISLGGMPEEDEARDYWFSEGFTDYLASKVLLRAGIWTLAEWAADKNETLLRYGQSP
                     AKTADAAEIARRFWTDAEVQQVSYDRGHLLAAKLDSEIAARSDGKQSLDSVLRAQRRA
                     AEGSPELAVALFRKTLRDETGIDIDPDLERYARRGEPLLLPAGLIGECGRVVTGHRKA
                     FTRGYDSEATRRGDGVIVGVDREGPAYAAGLRDGMRLVRRESGKIGDSSVELAYRVTD
                     GGVERVISYMPEAKAEFEVQRLELTASGPEAEAACRTRLGGAS"
BASE COUNT          572 a         1185 c         1289 g          571 t
ORIGIN      
        1 ttcagcaaag gttcgaagtt tcagcttcca tagaagcaga aaaaagaggc tgagactttc
       61 gctcggagac gccctacgtc cggaacgggg cggaaaacag aaaggcgatg ctcggcgcat
      121 gcagggaaca taaggcgaac atgatccgcc gtcaagggct tcgagagcgg cttggttcga
      181 aaggggcttg gctctatcct gacgccaggg ggatggcctg ccgtgctgaa atcgatcttt
      241 gccgtcatat cgctggcagc cgcctctttc ctgccgaacg gggcggcggc gcgcggagag
      301 gccacccagg tcgcgcacac gtcctggctc gggatccgga tcgtccagga cggtaagccc
      361 gtgcggctgg tcaggaccga cctgcgcacg accgaggccc gtctcgcgcc tcggccgttc
      421 cggatggtgt ttccgaagcg gcgggcggac gtcgcctacc agctctgcgc ctggaccggg
      481 aaatcgatct tccgggacct tcggccgcga gtcgttccgg ggaatcccaa tgccggcggc
      541 gagcatccct gcttcgcgcc cggcacggga atggcggata ccgaggccgg atcgggaatg
      601 ctgatgctga gcaactcagg caacaattac ctgatcgacc tcaggctggg tccggatccg
      661 gagcggccgt cggtttatta ttcggcgctt tggacgaggg cgcgggggca ggtcccgatc
      721 gcggcggcgc ggcggcccgt ctacatcgtc gccttccacg attccgacgg cgacggattt
      781 atggccaaca gcgaatatga tcatctggtg ctgaggttcg cgcggtaacg cactaaattc
      841 ctcccctgcc tttgcagggg agtgggacca tgcgaagcgt ggtggagggg ccttgccgga
      901 tccgaagaac ccctccaccg cgcgtcgcgc ggtccccctc ccccggaaat ccgggggagg
      961 aatttagtcg tagacgatcc tcacctctgc cgccgctcgc gtcgccaagg cccgcgcctc
     1021 ctccgccgtt gcacccgtcg caagcgccac gcccatccgg cggtacggcc tcgtcacggg
     1081 cttgccgaag attcgcacgt ccacttccgc gccctcgctc cccagcgcca gagcctcggc
     1141 cacaccttcg aagcggaagc tctccgactc ccgatccgcc aggatcacgg cgctcgcggc
     1201 ggcgggggcg gtgaggccta tgtgggggat cgggaggccg aggatcgccc gggcgtggag
     1261 gtcgaattcg gacaggttct gcgagatgag ggtcaccatc ccggtgtcgt ggggccgcgg
     1321 cgacaattcg gagaagatga cctcgtcgcc ggcgatgaag aactcgactc cgaacaggcc
     1381 gaagccgccg agatcgtcga ccaccttgcg cgccatgtcc cgggcctcgg cgagcgcgcg
     1441 gggagacatt ggggtgggct gccagctttc gcgataatcg ccgcgctcct ggcggtggcc
     1501 gatcggcggg cagaagagaa cgccttcgcg ggtccggacg gtgagcaatg tgatctcata
     1561 gtcgaagcgg acgaactgct cgacgatcac ccgcgcccgg tcgccgcgca tgttggccac
     1621 cgcatagtcc caggcggagc gaagtccgtc ggcggcctcg acggtaagct ggcccttgcc
     1681 ggacgaggac atgaccggct tgacgacgca ggggaagccg gtgtgggcgg cggcggcttc
     1741 gacctcctcg aagctctccg cgaagcggta gagcgaggtg cgaaggccga gcggaccggc
     1801 ggcgagctcg cggatggcgt cgcggttcat cgtcatcgtc gtcgcgcggg ccgtcgggac
     1861 gacatgaatt cccgcggact ccagctccgc cagcacctcc gtccggatcg cctcgatctc
     1921 gggcaccacg aaatcgggcc ggtgccgctc gatcgcctct cgcagcctct ccccatccag
     1981 catggagaat acctccgctt cgtcggcgac ctgcatggcg ggcgcgccgg cataggaatc
     2041 gcaggcgacc acccaggcgc cgaggcgctt ggcggagatc gcgaactcct tgccgagctc
     2101 gcccgatccg agcagcagga ccctggcggt gaagctcatg aggcgccgcc gagacgggtc
     2161 cggcaggcgg cctcggcctc gggcccggag gcggtcagct cgaggcgctg cacctcgaac
     2221 tcggctttcg cttccggcat gtagcttatc acccgctcga ccccgccgtc ggtgacccga
     2281 taggccagct cgaccgagga gtccccgatc ttgccggact cgcggcggac gagcctcatc
     2341 ccgtcgcgca gcccggcggc gtaagcggga ccctcgcggt cgactccgac gatcacgccg
     2401 tcgccgcggc gggtcgcctc cgagtcgtaa ccccgggtga aggccttgcg gtgcccggtg
     2461 accactcggc cgcactcgcc gatgaggccg gcggggagaa gcaggggttc gccgcggcgg
     2521 gcgtagcgct cgaggtccgg atcgatgtcg attccggtct cgtcgcgcag ggtcttgcgg
     2581 aacaaggcga cggcgagctc cgggctgccc tcggccgccc ggcgctgggc gcggagcacg
     2641 ctgtcgaggc tttgcttgcc gtcggagcgg gcggcgatct cggaatcgag cttggcggcg
     2701 aggaggtgac cgcggtcgta gctcacctgc tggacctcgg cgtcggtcca gaagcgccgg
     2761 gcgatctcgg cggcgtcggc ggtcttcgcg ggagactggc cgtagcggag cagggtctcg
     2821 ttcttgtcgg ccgcccattc cgccagcgtc cagatgccgg cgcgaagcag caccttggag
     2881 gcgagatagt cggtgaagcc ctcgctgaac caataatccc gcgcctcgtc ctcctccggc
     2941 attccgccga gcgagatcgg cacccagctg tgcatatatt cgtggccgag gaagcggacc
     3001 gcctgcctga gctcgaacgc gctcgttgag gcgatcgaga aggcgtccgt tcggccggcg
     3061 ccgacgtagg agaggccgga ggggatgtcg ccgagcgggg ccatggcgat gaggaagggt
     3121 ccggagcggt cgccccagaa cgaatcctcg gcggcgacga tgggagtcac ggtgtcggca
     3181 agctggtcgg gcgtgaactt ccagtcgccg agcagtgcga ctcgcagctc ggcgccctgc
     3241 aggtcgcggc ggacgacgcg cagcttcgcg ccgccgatgg cgacgctgtt gatgaggttg
     3301 gccagagtag tcggctggcc tttgagatgg tcgaggtcgg acgcgaccgt ccagcctttc
     3361 ggaagcttgc cccatctgaa cttcgccggg cggtcgtcgg ccccgcccgg gtccgcgaag
     3421 agggcctcgc cgtggacgaa gaaccagtcg ggccggatga gcggccgcgg ctgttcgaag
     3481 tcgaagccgg gatcctccgc ataggccgag acgatccggt agcggacccg gaggcgggcg
     3541 ccggggcggt ggcgggcgag gggcttgtcg taggtgccgg cgaggctttt cgcgcctttt
     3601 atctcgaggc ccacggc
//
LOCUS       DASWNU010000125         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_445890, whole genome shotgun sequence.
ACCESSION   DASWNU010000125 DASWNU010000000
VERSION     DASWNU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445890"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGB57_02410"
     CDS             complement(<1..511)
                     /locus_tag="VGB57_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0130230.1"
                     /translation="MTSGLAIEARGLFKAFDGKTAVDGIDLAVPEGSIYGILGPNGAG
                     KTTTLRMLLGIIDPDRGERSLLGRPHPLEAARLVGYLPEERGLYPAMTAREAVAFMGA
                     LRGLPLATGRARAEELLKANGLSDYVDKPIKSLSKGMAQTVQLFGTIVHRPKLIVLDE
                     PFSGLDAINQ"
     gene            549..1655
                     /gene="queG"
                     /locus_tag="VGB57_02415"
     CDS             549..1655
                     /gene="queG"
                     /locus_tag="VGB57_02415"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY0130231.1"
                     /translation="MSEGKPKLAELRERLEEKALELGFCAIGFARSDSAPRSGERLRQ
                     WLEEGAHGDMIWMEETAERRASPAGLWPEVRSIISLGMSYAPAVDPLALAGRPEIGRI
                     SVYAQGADYHDLVKKALKALARWLVAEAGCELKVFVDTAPVMEKPLAEAAGLGWQGKH
                     TNLVSRSDGSWLFLGAIYTTLELEERAASHAVHCGSCSACLDACPTNAFPGPFRLDAR
                     RCISYLTIEHKGPIPEEFREAIGNRIYGCDDCLAVCPWNRFADAARANKAFLPRAELV
                     APRLADLLALDESAFRKVFAGSPIKRIGRGRMVRNAAIAAGNSGLPELAPVLERLTGD
                     EDPVVAEAARWGLERLLASPSKMGRGTTRRVVEG"
     gene            complement(1765..2142)
                     /locus_tag="VGB57_02420"
     CDS             complement(1765..2142)
                     /locus_tag="VGB57_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130232.1"
                     /translation="MAGQGRIWLGALLLLGGCATAKTDGTAPAVNRPPPQRVVPYTST
                     GLENVLGRTARMLEAQFGKPGLDVREGPARKLQFGGPACVLDAYLYPPKGGGEPIVTH
                     IDARLRDGRDLDRASCVAALTRR"
     gene            2308..3306
                     /locus_tag="VGB57_02425"
     CDS             2308..3306
                     /locus_tag="VGB57_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY0130233.1"
                     /translation="MTDKSLDVLCIGNAIVDVIAAADDDFLKQEGLDKGSMRLIDAEE
                     ATRLYGRMGPGREISGGSAGNTAAGVAMLGGRAGFIGQVADDQLGEVYRHDVCSIGVE
                     FTTPARPQAEAPTARSLVLVTPDAQRTMNTFLGAAQNLSSEALDEAQLRSARILYLEG
                     YLWDPEVPRAAMERAIGITRDAGGAVAFTLSDSFCIERHRDSFNALIDSGRLDILFAN
                     EAEIVSLTGEADFDAAIDSAAKRVSVLVVTRGEKGAVAVEGGEQVEVPAEPVERIVDT
                     TGAGDLFAAGFLTGRAQGRSNLESLRMGAIAAAEVISHYGARPEADLKALVAERLG"
     gene            complement(3345..>4095)
                     /locus_tag="VGB57_02430"
     CDS             complement(3345..>4095)
                     /locus_tag="VGB57_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0130234.1"
                     /translation="MLVTAGAVGAMGGQPVMGANGIPLPTGEALSLQCATPQYAEALV
                     CSREPLAHVLHTIGYGSIGNMLGYAAFIALPSVILILLFGQTRIFFVMARDGLLPEAL
                     SRVHPRWKTPHVVTIITGLIVAVAAAFFPVGALADVANAGTLYAFLMVAIAVWQLRKT
                     DPDRRRAFRVPAVGLVALLTVGGCLFLFFNLPREAMLVLPAWTLIGVVFYALYGYRKS
                     HLGRGIVEVPEADPDAPMPVGIRPPDGSYDN"
BASE COUNT          645 a         1367 c         1488 g          595 t
ORIGIN      
        1 cctgattgat cgcgtcgagg cccgagaagg gctcgtcgag gacgatgagc ttcgggcggt
       61 ggacgatggt gccgaacagc tggacggtct gcgccatgcc cttggacagg ctcttgatcg
      121 gcttgtcgac ataatcggag aggccgttgg ctttcagcag ctcctcggcc cgagcccttc
      181 ccgtggcgag gggaaggccg cgaagggcgc ccatgaaggc gaccgcctcg cgcgccgtca
      241 tcgccggata gaggccgcgc tcctcgggca gatagccgac gagcctggcc gcttcgagcg
      301 ggtggggccg gccgagcagc gagcgctcgc cccggtcggg atcgatgatc ccgagcagca
      361 tccgcaaggt cgtcgtcttg ccggcgccgt tcgggcccag gattccgtag atgctgcctt
      421 ccggaacggc gagatcgatg ccgtcgacgg cggtcttgcc gtcgaaggcc ttgaacaacc
      481 ctctcgcctc aatagctaag ccactcgtca cgcgtgcccc ctttgagcgc ctgtggtagc
      541 gagcgaggat gagcgaaggc aagccgaagc tggcggagct gcgcgagcgg ctggaagaga
      601 aagcgctgga gcttggcttc tgcgcgatcg gcttcgcgcg ttccgactcg gctccgagga
      661 gcggggagcg ccttcgccag tggctcgagg agggcgcgca tggcgacatg atctggatgg
      721 aggagaccgc cgaacggcgg gcttctccgg cgggcctgtg gccggaggta cgctcgataa
      781 tctcgctcgg catgagctac gcgcccgccg tcgatccgct ggctctggcc ggacggccgg
      841 agatcgggcg gatctcggtc tatgcgcagg gcgccgatta tcacgatttg gtcaagaagg
      901 cgctgaaggc gctcgcccgc tggctggtcg ccgaggcggg atgcgaactc aaggtgttcg
      961 tcgacaccgc tccggtgatg gagaagccgc tggcggaggc ggccgggctg ggctggcagg
     1021 gcaagcatac caacctcgtc agccggagcg acggcagctg gctgttcctc ggagcgatct
     1081 acacgacgct cgagctggag gagagggcgg cgtcccacgc cgtccattgc ggaagctgca
     1141 gcgcctgcct ggacgcctgc cccacgaacg cctttccggg tcccttccgg ctcgatgcgc
     1201 gccgctgcat ctcctacctt acgatcgagc ataaggggcc gatccccgag gaattccgcg
     1261 aggcgatcgg caaccggatc tacggctgcg acgattgcct ggccgtctgc ccctggaacc
     1321 ggttcgccga tgccgcccgg gcgaacaagg cgttcctgcc gcgcgccgag ctggtcgcgc
     1381 cgcggctggc ggacctgctg gccctggacg agtcggcgtt ccgcaaggtg ttcgcgggct
     1441 cgccgatcaa gcggatcggg cggggccgga tggtgaggaa cgcggcgatc gctgcgggga
     1501 atagcgggtt gccggagctg gcgccggtgc tggagcggct gacgggcgac gaggatccgg
     1561 tcgtggcgga ggcggcgagg tgggggttgg aacgattgct tgcctcccca tcgaagatgg
     1621 ggagggggac cacccgaagg gtggtggagg ggtgattggc gccggattca gcggttcgtc
     1681 cgaggtcctc taagtccctc gccttctacc cctccaccag gcttcgcctg gtccccctcc
     1741 ccatgcttcg catggggagg caaatcaccg cctggtcagc gccgcgacgc agcttgcccg
     1801 gtcgagatcc cgcccgtcgc gcaggcgcgc atcgatatgg gtgacgatcg gctcgccgcc
     1861 gcccttgggc gggtagagat aggcgtcgag gacgcaggcg gggccgccga actggagctt
     1921 gcgcgccggc ccctcgcgaa cgtcgaggcc gggcttgccg aactgggcct cgagcattcg
     1981 agcggttcgg ccgagaacgt tttcgaggcc ggtcgacgta taggggacga cccgctgcgg
     2041 cggcggccgg ttcaccgccg gcgcggtccc gtcggtcttg gcggtcgcgc agccccccag
     2101 gagcagcagc gcgccaagcc atattcttcc ttgcccagcc acggcgccct cccctgcctt
     2161 tcgccgcggc agagtggcgc agcgccgccc ggagagccac gcgggttttc gcccgcccgg
     2221 cccgccattg cctcgcctcg gcgcaattga cgcgcggcgc ggggcccggc tagggccgcc
     2281 gctcctcccg cagcagatga gaacaacgtg accgacaaga gcctcgacgt cctttgcatc
     2341 ggcaacgcca tcgtcgacgt catcgccgcc gccgacgacg atttcctgaa gcaggagggt
     2401 ttggacaagg gctcgatgcg cctgatcgac gccgaggagg cgacccggct ctacgggcgg
     2461 atggggcccg gccgcgagat cagcggcggc tcggccggaa acacggccgc gggcgtggcg
     2521 atgctgggcg ggcgggccgg cttcatcggc caggtcgccg acgatcagct gggcgaggtc
     2581 taccgccatg acgtctgctc gatcggcgtc gagttcacta ccccggcgcg gccgcaggcc
     2641 gaggcgccga cggcgcgctc gctcgtcctc gtcacgcccg acgcccagcg gacgatgaac
     2701 accttcctcg gagccgccca gaatctgagc agcgaggcgc tcgacgaggc gcagctgaga
     2761 agcgccagga tcctctacct cgaaggctat ctgtgggacc cggaagtgcc tcgggcggcg
     2821 atggagcggg cgatcgggat cacccgcgat gccggcgggg cggtcgcctt caccctctcc
     2881 gacagcttct gcatcgagcg ccaccgcgac agcttcaacg cgctgatcga cagcggccgg
     2941 ctcgacatcc tcttcgccaa cgaggcggag atcgtctcgc tgaccggcga ggcggatttc
     3001 gacgcggcga ttgattccgc ggcgaagcgg gtctccgtgc tcgtcgtcac ccgcggcgag
     3061 aagggcgcgg tcgccgtcga gggcggagag caggtcgaag tgccggcgga gccggtcgag
     3121 cggatcgtcg acacgaccgg cgccggcgac cttttcgccg ccggcttcct gaccggccgg
     3181 gcccagggcc gctccaacct cgagagcctg cgcatgggcg ccatcgccgc ggccgaagtc
     3241 atctcccatt acggggcgcg cccggaagcg gacctcaagg cgctggtcgc ggagcggctc
     3301 ggctgaaacg aagaaggggg gcccgaaagc ccccctcccc gagatcaatt gtcgtaggag
     3361 ccgtccggcg ggcggatccc caccggcatc ggcgcgtccg gatccgcttc cggcacctcg
     3421 acgatgccgc ggccgagatg gctcttccgg tatccgtaga gggcgtagaa gacgaccccg
     3481 atcagggtcc aggccggaag cacgagcatc gcctcgcgcg gcaggttgaa gaacaggaag
     3541 aggcagccgc ccacggtgag aagggcaacg agcccgaccg ccggcacccg gaaggcgcgg
     3601 cgccggtccg gatcggtctt gcgcagctgc cagaccgcga tcgccaccat caggaaggcg
     3661 tagagcgtcc ccgcattggc gacgtccgcc agcgcgccca ccgggaagaa ggcggcggcg
     3721 acggcgacga tgaggccggt gatgatggtc accacgtgcg gggtcttcca gcgcggatgg
     3781 acccgcgaca gcgcttccgg gagcaggccg tcgcgcgcca tcacgaagaa aatgcgggtc
     3841 tggccgaaca gcaggatgag gatgaccgag ggcagggcga tgaaggccgc gtagccgagc
     3901 atgttgccga tcgagccgta gccgatggtg tggaggacgt gggccaaggg ctcgcgcgag
     3961 cagaccaggg cctccgcata ttgcggcgtg gcgcactgca gcgacagagc ctcgccggtc
     4021 ggcagcggaa ttccattcgc tcccatgacc ggctgtccgc ccatcgcccc gaccgcgccc
     4081 gccgtcacca gcatg
//
LOCUS       DASWNU010000126         3808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_186929, whole genome shotgun sequence.
ACCESSION   DASWNU010000126 DASWNU010000000
VERSION     DASWNU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186929"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VGB57_02435"
     CDS             complement(<1..526)
                     /locus_tag="VGB57_02435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0130235.1"
                     /translation="MERLRTFVIVLVTAIVTCALTSAFWFMAYSGVSVSTGTPGPIAS
                     PPAPKDSTPRSELVLGPTGVAIPVAGVKGSELQDTYTQSRSGGARVHNAIDIMAPHGT
                     PVVAAAPGTVEKLFDSKGGGGITAYVRSPDGQWMFYYAHLQAYAPGLREGQAVKQGDA
                     IGTVGSTGNANPAGP"
     gene            574..1311
                     /locus_tag="VGB57_02440"
     CDS             574..1311
                     /locus_tag="VGB57_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0130236.1"
                     /translation="MTGSVHIGCSGWIYRHWRDLFYPAKLAQRLWFAHYADTFRTVEL
                     NTSFYHLPRPETFTKWRDQAPEGFRYAVKAPRFITHMKKLKDCAEPVEEFLSRARNLG
                     ATIGPILYQLPPRWAFNRERLEEFIALLPSDLTHVFEFREKSWLTEEVLALLDARGIS
                     FCAHDMPGSATSRWAAGPIAYVRFHGGEGKYWGRYSDEGLLAWTDWIAEQARGGRDVW
                     CYFNNDIGGHAIHDALTLRAMVGQAFR"
     gene            complement(1418..2122)
                     /gene="thiE"
                     /locus_tag="VGB57_02445"
     CDS             complement(1418..2122)
                     /gene="thiE"
                     /locus_tag="VGB57_02445"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0130237.1"
                     /translation="MRTPEAMEDPLDPEFARLFEPTARPPCQLYLISPLDVGGDFPER
                     LERALDGGPVAAFQFRVKGVDQHEAARLAGPLQRICETREVAFIVNDDMGLAKRLGAD
                     GVHLGQSDGDPREARALLGPSAQIGITCHDSRHLAMEAGDAGVDYVAFGAFFPTTTKE
                     TAHRPDPAILSWWSRIFEIPCVAIGGITSENGRALVEAGADFLAVCSSVWGHAQGPEA
                     AVAEFARLLSEERSRA"
     gene            complement(2109..2648)
                     /locus_tag="VGB57_02450"
     CDS             complement(2109..2648)
                     /locus_tag="VGB57_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0130238.1"
                     /translation="MNALALDCLRLERQDWVLEVGFGGGGLLRSLLLGTRGEVFGADL
                     SDALIARARDRFARDVRRGRLHLLKASVESLPLPPETVTRAVSVNSLYFWPDPAAAMA
                     ELARVMKPGGRLAIVFEPAAELRKWPGHRFGFRLFEVAEVKALMESAGFAEIAEQWGT
                     GRKPARFCCLSGERVDANA"
     gene            complement(2744..3628)
                     /locus_tag="VGB57_02455"
     CDS             complement(2744..3628)
                     /locus_tag="VGB57_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HEY0130239.1"
                     /translation="MTAQMEKGEGFIAALDQSGGSTPKALKGYGLEEGAWSSDEEMFG
                     LIHSMRSRIITSPAFGGDKVIGAILFERTMDGDIDGRPVPEVLKERGVVPFIKIDKGL
                     EAEENGVQLMKPMPDLDALLARARGLGVFGTKERSVINLANREGIAAIVAQQFEIARQ
                     VLAAGLVPILEPEVNIKSPERAEADRILLEELLKGLDSLPGDEKVMLKLSIPATPGLF
                     DPLVDHPRVLRVVALSGGFSRSEACAQLARNRGMIASFSRALLEDLRHQMSDEEFDRS
                     LGGAIDEIHRASTEKVAA"
BASE COUNT          595 a         1305 c         1274 g          634 t
ORIGIN      
        1 gggggccggc cggattggcg ttgccggtcg agccgacggt cccgatcgcg tcgccctgct
       61 tcaccgcctg gccttctctg agccccggcg cgtaggcctg gagatgggcg tagtagaaca
      121 tccactggcc gtcgggcgat cggacataag cggtgatccc gccgccgccc ttgctgtcga
      181 acagcttctc gaccgtcccg ggggccgccg cgaccacggg ggttccgtgc ggcgccatga
      241 tgtcgatcgc gttgtggacc cgggcgccgc ccgagcgcga ctgggtgtag gtgtcctgga
      301 gctcgctgcc cttcacgccc gcgaccggga ttgccactcc agtcggaccg aggacgagct
      361 ccgagcgcgg ggtcgaatcc ttcggcgccg gcggcgaggc gatcgggccg ggggtgccgg
      421 tcgaaacgct cactccgcta taggccatga accagaaggc gctggtcagg gcgcaggtga
      481 cgatcgccgt gacgaggacg atgacgaagg ttctcaagcg ttccaccccc gtctaacgca
      541 acttattcct cacccgttcc gttctgggtt cgaatgacgg gaagcgtgca tatcggctgc
      601 tcgggctgga tctaccgcca ctggcgcgac ctcttctacc cggcgaagct cgcccagcgc
      661 ctctggttcg cgcattatgc cgacacgttc cgcacagtcg agctcaacac cagcttctac
      721 catctgccca ggccggagac cttcaccaag tggcgcgacc aggcgccgga gggtttccgc
      781 tacgcggtca aggcgccgcg cttcatcacc catatgaaga agctcaagga ctgcgccgag
      841 ccggtcgagg agttcctgag ccgggcccgc aacctcggcg ccacgatcgg cccgatcctc
      901 taccagcttc cgccgcgatg ggctttcaac cgggagcggc tcgaggagtt catcgccctc
      961 cttccgtccg atctcaccca cgtcttcgag ttccgcgaga agagctggct caccgaggag
     1021 gtgcttgccc tgctcgacgc tcgcggcatc tctttctgcg cccacgacat gccgggctcg
     1081 gccacttcgc gttgggcggc ggggccgatc gcctatgtcc gcttccacgg cggcgagggc
     1141 aaatattggg gccgctattc ggacgagggg ctgctcgcct ggaccgactg gatcgccgag
     1201 caggcgcgag gcggccggga cgtctggtgc tatttcaaca acgacatagg aggccacgcc
     1261 atccacgacg ccctgacgct gagggcgatg gtggggcagg cgttccggta atccgtcatc
     1321 ccggcgaaag ccgggaccgg ggggagaccc gccgagactt cttgtcctga ggtcccggct
     1381 ttcgccggga tgacggtgga tggtcgaacg cgccggttca ggcccggctc cgctcctccg
     1441 agagcaagcg cgcgaactcc gccacggccg cttctggccc ttgcgcatgc ccccacacgg
     1501 agctgcagac cgcgagaaaa tccgcccccg cctcgaccag cgcccggccg ttttccgaag
     1561 tgatcccccc gatcgccacg cagggtatct cgaagatgcg cgaccaccag gagaggatcg
     1621 cgggatcggg ccggtgcgcc gtctccttgg tcgtcgtcgg gaagaaggcg ccgaaggcga
     1681 catagtcgac tccggcgtcc cccgcctcca tcgccaggtg acggctgtcg tggcaggtga
     1741 ttccgatctg cgccgagggg ccgagaagcg ccctcgcctc gcgcggatcg ccgtcgcttt
     1801 ggccgagatg aaccccgtcg gcgccgagcc tcttggccag ccccatgtcg tcgttgacga
     1861 tgaaagcgac ctcgcgcgtc tcgcagatac gctgcagcgg cccggccagc ctcgcggctt
     1921 catgctggtc gacgcccttc accctgaact ggaaggcggc gaccggcccg ccgtccaggg
     1981 cgcgctccag ccgctccggg aaatcgccgc cgacgtcgag cggggagatc aggtagagct
     2041 ggcagggcgg ccgggccgtc ggctcgaaca gccgggcgaa ttcgggatcg agcgggtctt
     2101 ccatcgcctc aggcgttcgc atcaacccgc tcgccgctca agcagcaaaa gcgggcgggc
     2161 tttcggccgg tcccccattg ctcggcgatt tcggcgaagc cggccgactc catcagcgcc
     2221 ttgacctccg cgacctcgaa cagccggaac ccgaaccggt gccccggcca tttgcgaagc
     2281 tcggccgcgg gctcgaagac gatggcgagg cgcccgccgg gcttcatcac ccgggcgagc
     2341 tcggccatag cggcggcagg gtcgggccag aaatagaggc tgttgacgct caccgccctg
     2401 gtcaccgtct cgggcggcag cggcaggctc tcgacggagg ctttgaggag gtgaagccgg
     2461 ccgcgccgga cgtcgcgcgc gaagcggtcg cgcgcccgtg cgatgagcgc gtccgaaaga
     2521 tcggccccga acacctcgcc gcgggtcccg aggagcagcg agcggagaag tccgccgccg
     2581 ccgaaaccca cctcgagcac ccaatcctgg cgttccaggc gcaggcaatc cagggccagc
     2641 gcgttcatgt cccggctgat ccggtcgagc cacggcgcga tcaggaagcg gccgacggga
     2701 cccgacggcc gcgcgaactg gcgggccagg aagcggtgga ggcttatgcc gccaccttct
     2761 cggtcgaggc gcggtggatc tcgtcgatcg ccccgcccag cgagcggtcg aattcctcgt
     2821 cgctcatctg gtggcgaagg tcttcgagca gcgcccggct gaagctcgcg atcattccgc
     2881 gattccgggc gagctgggcg caggcttcgg agcggctgaa gccgccggac agggcgacca
     2941 ctcgaaggac gcgcggatgg tcgacgagcg ggtcgaacag cccgggggtc gccgggatgc
     3001 tgagcttgag catcactttt tcgtcgccgg gaagcgaatc gaggcccttg agcagttcct
     3061 cgagcaggat tcggtcggcc tccgcgcgct cggggctctt gatgttgacc tcgggctcga
     3121 ggatcgggac caggccggcg gcgagcacct gccgggctat ctcgaactgc tgggcgacga
     3181 tcgcggcgat gccctcgcgg ttcgcgaggt tgatgaccga gcgctccttg gtgccgaaca
     3241 cgccgaggcc cctggcgcgg gcgagaaggg cgtccaggtc cggcatcggc ttcatcagct
     3301 ggacgccgtt ctcctcggct tcgagccctt tgtcgatctt gatgaagggc accacgcccc
     3361 gttccttcag cacttccggc accggcctgc cgtcgatgtc gccgtccatc gtccgctcga
     3421 acaggatggc gccgatcacc ttgtcgccgc cgaaggccgg cgaggtgatg attcgggagc
     3481 gcatggagtg gatgagcccg aacatctcct cgtcggagct ccaggcgccc tcttccaggc
     3541 catagccctt gagcgccttg ggcgtcgatc cgccgctctg gtcgagcgcg gcgatgaagc
     3601 cctcgccctt ctccatctgc gccgtcatct tcgcgtgatc catttggtct cgtcctctga
     3661 ttcggtggaa attgggcgtc ccgtatctca gcgagtggcg gggttcaaat cggcgcgatt
     3721 tgcttgaact tccttcttca aattttcctc gtgcccatag cctcaatacc tttgcgagaa
     3781 ggtcagacag gatggacaga cggccaaa
//
LOCUS       DASWNU010000127         3416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_315631, whole genome shotgun sequence.
ACCESSION   DASWNU010000127 DASWNU010000000
VERSION     DASWNU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315631"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGB57_02460"
     CDS             <1..439
                     /locus_tag="VGB57_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0130240.1"
                     /translation="FVVGFPGETEEDFQYLLDWLEEAQLDRVGAFRFEPVEGAAANAL
                     PDPVPEGVKQERFERIMALSARISAEKLAAKIGKSLEVLIDSVDKETGGATGRSKADA
                     PEIDGEVHLRDAGGLNPGDIVSVRIEDSDEHDLYGVPLGAALA"
     gene            449..1843
                     /locus_tag="VGB57_02465"
     CDS             449..1843
                     /locus_tag="VGB57_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY0130241.1"
                     /translation="MTDFASLLVADRGQKARAIHIVDKDSFAGWAKARNAEDKALLEA
                     HRFDGKSGYASVILPRGGDFEVVTGVANAASLSPWCLAKLPDTLPAGEYRLADGEPGK
                     AALGWLLAQHRFDAFRKKKESEPGPRVLVTGEPARIDATGRLAEATALVRDLVNTPAG
                     DLGPSELEQAVRDQVSRVNAEVRVTSGDALAEGYPLVMAVGAAASRERAPRLVEAEWG
                     DPGHRRVAIVGKGVCFDSGGLDLKNASGMRLMKKDMGGAAHALALFTLVASERLPVRL
                     HLLIPAVENAVSAAALRPGDIVKSRLGLQVEIDNTDAEGRLILADALARAAEDKPELI
                     LDFATLTGAARVALGPDLPALFANRDDLLEDIETAGKEVEDPLWRMPLWDGYDEMLKS
                     DIADLANATGSSMAGCVTAALFLRRFVPKEIAWAHLDTFAWRPKAKPGRPKGGDALGL
                     RALFELLSRRYPSR"
     gene            1990..2343
                     /locus_tag="VGB57_02470"
     CDS             1990..2343
                     /locus_tag="VGB57_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0130242.1"
                     /translation="MKALIAYVRSGEPDLTNRQMALLMLVYLTPGPHTVRGLAKILGV
                     SKPVVTRALNTLGALGYLRRERDQDDRRNVFVVRTSNGADFLEGFKRNLREGGDGAPA
                     AHRPIIGTAAFAHQR"
     gene            2471..2647
                     /locus_tag="VGB57_02475"
     CDS             2471..2647
                     /locus_tag="VGB57_02475"
                     /inference="COORDINATES: protein motif:HMM:NF036854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130243.1"
                     /translation="MVTSSASLRAGPSEETEAVAELEAGDPFAMLDNSLGWAWGYAGE
                     QQRVGYVRSEALGS"
     gene            complement(2649..2981)
                     /locus_tag="VGB57_02480"
     CDS             complement(2649..2981)
                     /locus_tag="VGB57_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130244.1"
                     /translation="MTDRRAGRGRLFLGSVLLAGAVAASLTGLFTNPLEEGTSPGTEH
                     VRASVVIFMRNSAIATLVLVALASLLLFPRPRLRHPLRDRLLIAVAAALAAFSVYRLV
                     WLQTAVGN"
     gene            complement(2978..>3416)
                     /gene="mltG"
                     /locus_tag="VGB57_02485"
     CDS             complement(2978..>3416)
                     /gene="mltG"
                     /locus_tag="VGB57_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0130245.1"
                     /translation="PTCPVNSREEAVTLASIVEKETGKPSERRTVAGVYCNRLRIGMK
                     LDADPTVIYPITKGKPLGRRIRRSELAADTGYNTYKKPGLPAGPIANPGRDSIAAVLD
                     PAPTKAIYFVADGTGGHVFAETFEQHRANVAKWYAIRRARGEM"
BASE COUNT          554 a         1164 c         1158 g          540 t
ORIGIN      
        1 gttcgtggtt ggcttcccgg gcgagaccga ggaggatttc caatatctgc tcgactggct
       61 cgaggaggcc cagctcgacc gcgttggagc cttccgcttc gagccggtcg aaggcgctgc
      121 ggccaatgcc ttgcccgacc cggtgcccga gggggttaag caggagcgct tcgagcggat
      181 catggctctg tccgcccgca tctccgccga gaagctcgcc gccaaaatcg gtaagtcatt
      241 ggaagtgctc atcgattcgg tcgacaagga aactggtgga gcgaccggtc gaagcaaggc
      301 cgacgccccc gaaatcgacg gcgaggtcca cctccgcgac gccggtgggc tcaaccccgg
      361 cgacatcgtt tccgtccgaa tcgaggacag cgacgagcac gatctctacg gcgttccctt
      421 gggtgcggcc ctcgcctaag gggcgggcat gaccgacttc gcatcgctgc tcgtcgccga
      481 ccgcggccag aaggcacgcg caatccacat cgttgacaag gacagttttg ccggctgggc
      541 gaaggcccgc aacgccgagg acaaagcgct gctcgaagcc catcgcttcg acggcaagag
      601 cggttatgcc agcgtgatcc tgccgcgcgg cggcgatttc gaggtcgtca ctggggtcgc
      661 caacgcggcg tcgctgtcgc cctggtgcct ggccaaactt ccggacacgc ttccggccgg
      721 ggaatatcgc ctggccgacg gcgagccggg caaggccgcg ctcggctggc tgctcgccca
      781 gcaccgcttc gacgcctttc gcaagaagaa ggagtccgag cccggcccac gggtgctggt
      841 caccggggaa ccggccagga tcgacgccac ggggcgcctg gcggaagcga ccgcgctggt
      901 ccgcgacctg gtcaacacgc ccgccggcga tcttggcccg tcggagctcg agcaggccgt
      961 gcgcgaccag gtttcgcgag taaacgcgga agtgcgcgtg acctcggggg acgcgcttgc
     1021 cgagggttat ccgctggtca tggccgtagg cgccgccgcc tcgcgcgagc gcgcgccgcg
     1081 gctggtcgag gctgaatggg gcgatccggg ccaccggcgc gtcgcgatcg tgggcaaggg
     1141 ggtatgtttc gacagcggcg gcctcgacct caaaaacgcc agtggcatgc ggctgatgaa
     1201 gaaggacatg ggcggagccg cgcacgcgct cgcactgttc accctcgtgg cgtcagagcg
     1261 gctccccgtt cggcttcacc tgctcattcc ggcggtcgag aatgccgtct cggccgcagc
     1321 cctgcggccc ggcgacatcg tcaagtcgcg cctcggcttg caggtcgaga tcgacaacac
     1381 cgatgccgag gggcgcctga tccttgccga cgcgctggcc cgggcggcgg aggacaagcc
     1441 cgaactgatt ctcgatttcg cgaccttgac cggcgctgcc cgggtcgcgc tcgggccgga
     1501 cctgcccgcg ctgttcgcca accgcgacga tctcctcgaa gacatcgaga cggccggcaa
     1561 agaggtcgag gaccccctat ggcggatgcc gctctgggac ggctatgacg agatgctcaa
     1621 gagcgacatc gccgatcttg ccaacgccac gggctcttcg atggccggct gcgtcaccgc
     1681 cgctctgttc ctgcgccgct tcgtccccaa ggagatcgcc tgggcccatc tcgacacgtt
     1741 cgcctggcgg cccaaggcca agcccggccg tccgaaaggc ggcgatgcgc tcggcctcag
     1801 ggccttgttc gagcttctgt cccggcgcta cccgagccgc tgatccggtc gccccgtccg
     1861 atgatccgcg cgtttttccc gattttccgg tcgtttggct gcaacatttc gcccccttac
     1921 cggtttaatc gagcaagcac gcgacgccac ggagacaatc gaatgagcga ggtttcgcta
     1981 accggctgga tgaaggcgct gatcgcctat gtccgatccg gcgagccgga tctcaccaat
     2041 cgccagatgg cgctgctgat gctggtctat ctgacccccg gtccgcacac cgtgcgcggc
     2101 ctcgccaaga tcctcggagt gtccaagccg gtcgtcactc gcgccttgaa cacgctcggc
     2161 gcactcggct atctcaggcg cgaacgcgac caggacgacc gccggaacgt gttcgtcgtc
     2221 cggaccagca acggggcaga tttccttgaa ggcttcaagc gcaacctccg cgagggcggc
     2281 gacggggccc cggcggcgca ccggccgatc atcggcactg cggccttcgc ccaccagcgc
     2341 tgagggcttc gcgctcgccg gaccgtcgaa cccgccggac cccgattaca acgcctaccg
     2401 ccgcgacctc gccgacgtcg cgctcgccca cgccgtcatc gcgtcgcatt atgccgagcc
     2461 gcttgatcgt gtggtaacca gttccgcgag cctgcgcgcc ggcccatccg aggagacgga
     2521 ggccgtggcc gagcttgaag ccggcgaccc gttcgcgatg ctcgacaaca gcctcggctg
     2581 ggcgtggggc tacgccggcg agcaacagcg tgtcggctat gtccggagcg aggcgctcgg
     2641 cagctagatc aattgccgac ggccgtttgc agccagacca gacgatagac gctaaatgcg
     2701 gccagcgccg ccgcaacggc gatcagcagc ctgtcgcgca gcggatggcg gagacgcggc
     2761 cgcggaaaca gcaacagaga ggcgagtgcc acgagcacca gggtcgcgat ggcggagttg
     2821 cgcatgaaga tgacgaccga ggcgcggaca tgctccgtcc cggggctcgt gccctcctcg
     2881 agcgggttgg tgaacaggcc cgtcagtgac gcggcaacgg cgccggccag aaggacggaa
     2941 ccgaggaaca ggcggccgcg gccggcgcgc cgatcggtca catctccccc cgggcgcggc
     3001 ggatcgcgta ccatttcgcg acgttcgcgc ggtgttgctc gaaggtctcc gcgaagacat
     3061 ggccgccggt gccgtcggcg acgaaataga tggccttggt cggcgccggg tcgagcacgg
     3121 cggcgatgct gtcgcggcca gggttggcaa tcgggccggc tggcagcccc ggtttcttgt
     3181 aggtgttgta accggtgtcc gcagcaagct ccgagcggcg gatccgccgg cccagcggct
     3241 tgcccttggt gatcgggtag atgaccgtcg gatcggcatc gagcttcatg ccaatccgca
     3301 gccggttgca gtagacgccc gcgaccgtcc gccgttcgga gggcttgccg gtttccttct
     3361 cgacgatcga ggcgagcgtg acagcctcct ctctggagtt caccgggcag gtcggc
//
LOCUS       DASWNU010000128         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_30889, whole genome shotgun sequence.
ACCESSION   DASWNU010000128 DASWNU010000000
VERSION     DASWNU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30889"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..334
                     /locus_tag="VGB57_02490"
     CDS             200..334
                     /locus_tag="VGB57_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130246.1"
                     /translation="MRRRWEKRALASRRPLEESRGGWALFGRLLVWSTRNNRRRPEAG
                     "
     gene            493..>1660
                     /locus_tag="VGB57_02495"
     CDS             493..>1660
                     /locus_tag="VGB57_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0130247.1"
                     /translation="MLLRLSRRPLAAAAIVALAIVGAWGAGGPLGAVLLGLIGGSTAT
                     LIAWRAYFFPETVLVREAPSEGREEWAHEILGALAEPMLLIEGGRVAVANGPAKRLLG
                     QWIEGQDVRLALRHPATLELLSRPAPPDREVEPIEVVGIGQAERSWLVRIATLEGGAR
                     LVHLVDRSEAAAAEKMRVDFVANASHELRTPLATLLGFIETLQDDGAGADKEIRSRFL
                     GLMFGEGKRMQTLIEDLMSLSRIEAERFKAPRDTVDLVQIVEEVRAGCAHLFEERSNT
                     LVVENEARSTIVQGDRGQLLQLVRNLIVNALKYGRAESQVTVRLDDDGPSQLRFAVID
                     RGEGIAVEHLPRLTERFYRVDAGRSRSVGGTGLGLAIAKHIVGRHRGRLDIRSKL"
BASE COUNT          249 a          545 c          605 g          261 t
ORIGIN      
        1 gcgttgccgc gatgatgcga cgcggcctgt cgctcgtcga ggcgcacctg atcctgcctc
       61 cgcgcggaag cgggttcggg cggctccgcg gcgacgagcc ggtccgccgc tgagcgccgg
      121 aaacggcccc ttcgccttcc gtcatcagcc tgcaacaccg atcctttagg cccgtcccgg
      181 cacgacggga gaaacaatca tgcgtcgaag gtgggagaag cgggccttgg catcgcgtcg
      241 tccgctggag gagagccggg gcgggtgggc gcttttcggc cggctgctcg tctggtcgac
      301 ccgcaacaat cgccggcggc ctgaagccgg ctgagcggcc gtccttgcgg cgtcagtccg
      361 gcggccgctg aggttcgttc cccggcgcag gccggggccc agcggagcct ccgccaaacc
      421 gggtcccagc ctcgccggga agcggtccac gaagcgttgg tcctgaatcc ctaccgcgct
      481 aaaggcgagt atatgctcct ccgcctctcc cggcggccgc tcgcggccgc ggccatcgtc
      541 gcccttgcga tcgtgggcgc gtggggcgcg ggcggcccgc tgggcgcggt gttgctgggg
      601 ctcatcggcg gctccaccgc gactctcatc gcctggagag cctatttctt tccggaaacg
      661 gtgctcgtcc gcgaggcccc gtccgaaggc cgggaggagt gggctcacga aatattgggg
      721 gcactggccg agccgatgct gctgatcgaa ggcggacggg tggccgtcgc caacggcccg
      781 gcaaagcggc tgctgggcca gtggatcgaa ggccaggacg tccgcctcgc tttgcgccat
      841 ccggcgaccc tggagcttct ttcccgtccg gcgccgccgg atcgcgaggt cgagccgata
      901 gaggtggtcg ggatcgggca ggcggagcgg agctggctgg tgaggatcgc gaccctcgag
      961 ggaggcgcgc ggctcgttca cctcgtcgac cgaagcgagg ccgccgccgc ggagaagatg
     1021 agggtcgatt tcgtcgccaa cgccagccac gagctgcgca cgccgctggc gaccctgctc
     1081 ggcttcatcg aaaccctgca ggacgacggc gccggcgccg acaaggagat aaggtcgcgc
     1141 ttcctcgggc tgatgttcgg cgaggggaaa aggatgcaga ccctgatcga ggatctgatg
     1201 tcgctttccc ggatcgaggc ggaaaggttc aaggcgccgc gcgacacggt cgatctcgtg
     1261 cagatcgtcg aggaggtgcg ggcgggctgc gcccacttgt tcgaggagcg gtcgaacacg
     1321 cttgtcgtcg agaatgaggc ccggtcgacg atcgttcagg gggaccgtgg gcagctgctc
     1381 cagctcgtcc gcaacctgat cgtcaacgcc ctgaaatatg gaagggccga atcgcaggtg
     1441 acggtgcgcc tggatgatga cgggccttcc cagctgcgct tcgccgtcat cgaccggggc
     1501 gaaggcattg cggtcgagca tctgccgcgc ctgaccgagc gcttctaccg ggtcgatgcc
     1561 ggacgcagcc gctcggtcgg cggaaccggc ctgggcctgg cgatcgccaa gcatatcgtc
     1621 gggcggcacc gcggccggct cgatatccga agcaagctcg
//
LOCUS       DASWNU010000129         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_5254,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000129 DASWNU010000000
VERSION     DASWNU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5254"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..807
                     /locus_tag="VGB57_02500"
     CDS             79..807
                     /locus_tag="VGB57_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY0130248.1"
                     /translation="MDAVEEPGGRGDAEGRNLTFGMLEALGRAIVTGAYESRRFPTEA
                     ELAVQHMVSRSVTREAVKMLTAKGLLTARPRKGTTVQPSSSWNLFDTDVLRWLLERKF
                     SLELLRQFSELRIAIEPEAAALAAREANPAAVAEIRSGYERMEAAEAAGEDTLEADIA
                     FHIAVLNASANPFYMQFRDVVATALRTSIRFTNRFKGRTASLPAHHAVLAAIEGADCG
                     ASRSAMGAIIAEVMAMIDDAERAG"
     gene            901..>1931
                     /locus_tag="VGB57_02505"
     CDS             901..>1931
                     /locus_tag="VGB57_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0130249.1"
                     /translation="MPVYPVLSRPLAGLLLTAAAAAPALAAEPSFVPVFRENFPDPHI
                     VRHDDEFIAYATNAGINLPMLTSRDLVHWTWVSGADGKRRDGMPELAPWVKEGFTWAP
                     EVMKVGGKWLLYYTAAHRKRNVQCLGVASADNPKGPFRDGSAEPLVCQFELGGTIDAN
                     PFRDTDGKLYLYYKSDGNRVGKKTVIWGQRLSEDGLSVVGSPAALVEDDRKWEWKLVE
                     APTMVRSPAGHQLFFSAAFFGWDPKERLSRYATGYATCAGPLGPCRDAPENPILHSFN
                     DKTAGCLSGPGHTSIFEARGRHFIAFHAWEATRGCRKADEKRYLYVAPLFWKDGKPQI
                     GSSLRETGK"
BASE COUNT          327 a          666 c          641 g          297 t
ORIGIN      
        1 cgtcgccgcg ctgttgccgc ccgggccaag gaattatagg attaaatgga ctagtccggc
       61 ggggagcatg gggagaggat ggacgccgtg gaggagcctg gcggacgcgg cgacgcggag
      121 ggccgcaacc tcaccttcgg gatgctggag gcgctcggcc gggcgatcgt cacaggcgcc
      181 tacgagagcc ggcgcttccc gaccgaggcg gagctggcgg tccagcatat ggtgagccgc
      241 tccgtcaccc gcgaagcggt caagatgctc accgccaagg gcctgctcac cgcccggccc
      301 cgcaagggca ccacggtcca gccctcttcg tcctggaatt tgttcgacac cgacgttctg
      361 cgctggctgc tcgagcgcaa attctcgctc gaactgcttc gccagttcag cgagctcagg
      421 atcgcgatcg agccggaggc ggcggcgctg gcggcgcgcg aagccaatcc cgccgccgtg
      481 gccgagatac gctcgggcta tgaacggatg gaggccgcgg aagccgccgg cgaagacacg
      541 ctcgaagcgg atatcgcctt tcacatcgcc gttctcaacg cttcggccaa tcccttctac
      601 atgcagttcc gcgacgtggt ggcgaccgcc ttgcgcacct cgatccgctt caccaaccgc
      661 ttcaagggcc gaaccgcgag cctccccgcc catcatgcgg tgctcgcggc gatcgagggc
      721 gccgattgcg gagcgtcgcg aagcgccatg ggggcgatca tcgccgaagt gatggcgatg
      781 atcgacgacg ccgagcgcgc cggctaaacg cgccccgaac gggcaagaag ctttgttggc
      841 gggcggtgcg ccgtgctaca ggcggaaccg gcttcattcg catcgacagg atcattcgcg
      901 atgccggtct accccgttct ttcccgcccc ctggccggcc tcctgctgac ggccgccgcg
      961 gccgcgccgg ccctggccgc cgagccttct ttcgtgccgg tgttcaggga aaatttcccc
     1021 gacccccaca tcgtccgcca cgacgacgag ttcatcgcct acgcgaccaa tgccggaatc
     1081 aacctgccga tgctgacctc gcgcgacctc gtccactgga cctgggtgag cggcgccgac
     1141 ggcaagcgcc gcgacggaat gcccgagctc gcgccctggg tgaaggaggg cttcacctgg
     1201 gcgccggagg tgatgaaggt cggcggcaag tggctgctct actacacggc cgcccatcgc
     1261 aagcggaacg tccagtgcct cggagtggcc tcggccgaca atcccaaggg ccccttcagg
     1321 gacggctccg ccgagcctct ggtctgccag ttcgagctcg gcgggacgat cgacgccaac
     1381 cccttccgcg acacggacgg caagctctat ctctattaca agagcgacgg gaaccgggtc
     1441 ggcaaaaaga cggtgatctg ggggcagcgg ctctcggagg acggcctttc cgtcgtcggc
     1501 tcgccggccg ctctggtcga ggacgatcgg aaatgggaat ggaagctggt cgaggcgccg
     1561 accatggtgc gctcgccggc ggggcaccag ttgttcttct ccgcagcctt cttcggctgg
     1621 gatccgaagg agcggctgtc gcgctacgcc acgggctatg cgacgtgcgc cggcccgctc
     1681 ggcccctgca gggacgcgcc cgaaaatccg atcctccaca gcttcaacga caagaccgcc
     1741 ggctgcctca gcggccccgg gcacacgagc atcttcgaag cccgcggccg ccacttcatc
     1801 gccttccacg catgggaggc gaccaggggc tgccgcaagg cggacgagaa gcgctacctc
     1861 tatgtcgcgc ctctgttctg gaaggacggc aagccgcaga tcggctccag cctgcgcgag
     1921 acggggaagt a
//
LOCUS       DASWNU010000130         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_445943, whole genome shotgun sequence.
ACCESSION   DASWNU010000130 DASWNU010000000
VERSION     DASWNU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445943"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGB57_02510"
     CDS             complement(<1..621)
                     /locus_tag="VGB57_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEY0130250.1"
                     /translation="MRLDQTAQWQPTPAQYQAPGGPPEIPPPGPPRRAWYNPLRWGWR
                     RIVLALLALFMLIVAWLAITAPLSKSLQPIAAPSLTLVSVEGHPIARRGADIREPVKI
                     SQLPKHVPAAFISIEDRGFRGHLGISIRGIARAAWRNLGAGGVREGGSKITQQLAKIS
                     FLTSERTMGRKAQEVLIAFWLEAWLSKDEILERYLSNVYLGDNTYGL"
     gene            complement(792..1643)
                     /locus_tag="VGB57_02515"
     CDS             complement(792..1643)
                     /locus_tag="VGB57_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0130251.1"
                     /translation="MWDATTIDLSPDRSLHALQGGTGPDVLMLHGALNTHYDWLTGPA
                     GAIAEGARVTLLDRPGHGLSRRPRFVGTPRDQAAQIASGLDRLGVGPAVVAGHSFGAL
                     VSLALAERFPERVAALVLVAPLAFPEPRLLEHSLLAPRSVPVVGPLLSRLGNRSGFDR
                     AALEWLQELMFSPDAMPAHWKQGFPYEHVLDPDTLVHEGEDAAAVLPLSPTGTMDLRR
                     IEAPVHILAGTSDRIVQQEGQGKALARLLRNARLTEIEGAGHMLHHSHPDLVVRAVRE
                     AAAPVTA"
     gene            complement(1678..2496)
                     /locus_tag="VGB57_02520"
     CDS             complement(1678..2496)
                     /locus_tag="VGB57_02520"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0130252.1"
                     /translation="MADEERRRKGCLIIIGGHEDRDPKGERAILREVAKHVHGGKLVL
                     ATVASHRPEGYFDEYEKAFGDLGLGDLVELYVEERSRAGDRDKLSVLDDAAGIFFSGG
                     DQLRITSQIGDTGIEAKVRALYERGGVVAGTSAGASVMSETMLVKGTSKETHRIGDLH
                     MSPGMGLVRNVIIDQHFAERGRFGRLIGAVAHNPRVLGLGIDEDTAAMLEGDVFRVIG
                     SGAVYVVDGTDVSYCNVAEARSDCALSMHDVRVHVLSDGDSFDLKARRPIHPRS"
     gene            complement(2489..>3143)
                     /locus_tag="VGB57_02525"
     CDS             complement(2489..>3143)
                     /locus_tag="VGB57_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY0130253.1"
                     /translation="MCSGLMDGRDLSVGAVGAIRNVRNPVSVAKRLLPEKEILLVGEG
                     ATMFAKKAGVEMVPEEALMTETRREALEQMHDTVGAVAMDKGGNLAAGTSTGGLTGAK
                     VGRIGDSPLPGNGLYADNHVGAVSFSGDGESIARLALAAQVMASIGRDGPEEAVRKAV
                     EQLPGVGGSGGDGGGIAIAKDGRIGWAHNSSAFAIAYASGDSREPKAFLSKEEERRG"
BASE COUNT          492 a         1060 c         1093 g          498 t
ORIGIN      
        1 aaggccgtag gtgttgtcgc cgagatagac gttggagaga tagcgttcga ggatctcgtc
       61 cttgctgagc caggcttcga gccagaaggc gatcaggact tcctgggcct ttcggcccat
      121 cgtccgctcg gaggtgagga aggagatctt ggcgagctgc tgggtgatct tgcttccgcc
      181 ttccctcacc ccgccggcgc cgaggttgcg ccaggcggcc cgggcgatgc cccggatgct
      241 gatcccgaga tggcctcgaa agccgcgatc ctcgatcgag atgaaggccg cagggacatg
      301 cttcggcagc tgggaaatct tcaccggctc gcggatgtcg gcgccccgcc gggcgatcgg
      361 atggccttcc accgaaacga gggtgaggct cggcgccgcg atcggctgaa gcgacttgga
      421 aagcggcgcc gtgatcgcca gccaggcgac gatcagcatg aacagggcga gcagggcgag
      481 gacgatgcgc cgccagcccc agcgcagcgg gttgtaccag gcgcgccgcg gcgggcccgg
      541 gggcggtatc tcgggtgggc ctcccggggc ctgatactgg gccggggtcg gttgccactg
      601 ggcggtctgg tccaaacgca ttccttcttc ttagcggcag aacgacggcg cgggaatcag
      661 gttgcgcggc ccgtgccaac atctgcccat aattccgccg tcatcccggc gaaagccggg
      721 accctcgggg agacccaccg agacttcttg tcctgagatc ccggctttcg ccgggatgac
      781 gacgcggcgt ttcatgccgt gacgggagcg gcggcctcgc ggacggcccg gacgacgaga
      841 tcgggatggc tgtgatggag catgtggccg gcgccctcta tctcggtgag gcgggcgttg
      901 cgcaagagcc gcgcgagcgc cttgccctgg ccctcctgct ggacgatccg gtcgcttgtg
      961 ccggcgagga tatggaccgg cgcctcgatc cggcggagat ccatcgtccc ggtcggcgac
     1021 agcggcagga ccgcagccgc atcctcgccc tcgtggacca aggtgtcggg atccagcacg
     1081 tgctcgtagg ggaagccctg cttccagtgg gccggcatgg catcgggcga gaacatcagc
     1141 tcctgcagcc attccagcgc ggcccggtcg aagccgctcc ggtttccgag gcgagacagg
     1201 agcggaccga ccaccggcac cgatcggggc gcgaggagac tatgctcgag gaggcgcggc
     1261 tcgggaaagg cgagcggcgc caccagcacc aaagcggcga cccgctccgg gaagcgctcc
     1321 gccaaagcga gggagacgag ggcgccgaag ctgtgtccgg cgaccaccgc cggaccgact
     1381 ccgaggcggt cgaggccgga agcgatctgg gcggcctggt cgcgcggagt gccgacgaag
     1441 cgcggacggc ggctgagacc gtggccgggg cggtcgagga gggtgactcg cgcaccctcg
     1501 gcgatggcgc cggcaggccc cgtcagccag tcataatggg tgttgagcgc gccatggagc
     1561 atcagcacgt ccggaccggt tccaccctgc agcgcgtgaa ggctccggtc cgggctcaga
     1621 tcgatggtgg ttgcatccca catgacggcc caaccggcga gagaggctat ggctccgtca
     1681 acttcgcgga tgaatggggc ggcgggcctt gaggtcgaat gaatccccgt cggagaggac
     1741 gtggacccga acgtcgtgca tcgagagggc gcagtcggag cgggcctcgg cgacgttgca
     1801 atagctgacg tcggtgccgt cgacgacata gacggcgccg ctcccgatca cccggaagac
     1861 gtcgccttcg agcatcgccg ccgtatcctc gtcgatgccg aggccgagca cccgggggtt
     1921 atgggcgacg gcgccgatca gccggccgaa gcgaccgcgc tcggcgaaat gctggtcgat
     1981 gatgacgttg cggacgaggc ccatgccggg agacatgtgg aggtccccga tccggtgggt
     2041 ctccttgctg gtgcccttga ccagcatcgt ctcgctcatc accgacgcgc ccgccgaagt
     2101 cccggcgacg actccgccgc gctcgtagag ggctcggacc ttggcctcga tgccggtgtc
     2161 gccgatctgg ctggtgatcc gaagctggtc gccgccggag aagaagatgc cggcggcgtc
     2221 gtcgagcacc gagagcttgt cccggtcgcc cgcgcgcgaa cgctcctcga catagagctc
     2281 gaccagatcg ccgaggccga gatcgccgaa cgctttctca tattcgtcga aatagccttc
     2341 cgggcgatgg ctggcgacgg tggcgaggac gagcttgccg ccatggacgt gcttcgccac
     2401 ctcgcgcagg atcgcccgct cgcccttcgg atcgcggtcc tcatggcccc cgatgatgat
     2461 caggcagccc ttccggcgcc tttcctcgtc agccacgtct ctcttcttcc ttgcttagaa
     2521 atgccttggg ctcgcgcgag tcgccgctcg cataggcgat cgcgaaagcc gagctgttat
     2581 gcgcccagcc tatccgcccg tccttcgcga tagcgatgcc gccgccgtcg ccgcccgagc
     2641 cgccgacgcc cggaagctgc tcaaccgctt tcctgaccgc ttcctccggc ccgtcccgac
     2701 cgatgctcgc catcacctgc gcggcgagcg cgaggcgggc gatgctctcg ccgtccccgg
     2761 agaaggagac cgcgccgaca tgattgtcgg cataaaggcc gttgcccggc agcgggctgt
     2821 caccgatccg gcccaccttg gcgccggtga ggccgccggt cgaggtgccg gcggcaagat
     2881 tgcccccttt gtccatcgcc accgccccga ccgtgtcgtg catctgctcg agagcctcgc
     2941 gccgggtctc ggtcatcagc gcttcttcgg ggaccatctc gacgcccgct ttcttcgcga
     3001 acatggtcgc gccctcgccg acgaggagga tctccttctc gggcagcagc cgcttggcga
     3061 cgctgaccgg gttgcggacg ttgcggatcg cgccgaccgc tccgaccgac aggtcgcgtc
     3121 catccatcag gcccgagcac atc
//
LOCUS       DASWNU010000131         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_393707, whole genome shotgun sequence.
ACCESSION   DASWNU010000131 DASWNU010000000
VERSION     DASWNU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393707"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VGB57_02530"
     CDS             complement(<1..1434)
                     /locus_tag="VGB57_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0130254.1"
                     /translation="MKLSWFGRALAAAALLVASGPASAVDPLPADAKATPDPAVRTGT
                     LPNGLRYAIMRNASPARAVSIRLAIRAGSYDEEEAEGGFAHFIEHMAFRSTRQAPEGS
                     LDTRFAGLGVAFGRDENAVTGLESTVYQMDLPAADPAAVSKVLEWLRGAADGILFAPA
                     AVELEKGVVVAEIRARSNPASIVGREAARFQAKELRSFARDIGGTEESVRAAAPAALQ
                     AFYDRWYRPDNAVLVIAGDADPDALLKAAQAAFGSWTGKGAPGTRAKPASLPQRGLDA
                     FTRSGASLPLAESACRVAPLDGPRDSSLERMRRDALSQIWVTILTDRASQATTRTGSS
                     LLGTAPMVNRSLPDARVACLIAVPNQGKWREGLAEAQAELRRFAETGPTPLEVETAAE
                     QLRSRLRAAVYQSATRGTRELAQGIADAELAGTPYLHPREAMRVYDLLVAGLTPADVK
                     RAFEADWSGNGPLLVLTGPEEVSPAELA"
     gene            complement(1495..1923)
                     /gene="mscL"
                     /locus_tag="VGB57_02535"
     CDS             complement(1495..1923)
                     /gene="mscL"
                     /locus_tag="VGB57_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY0130255.1"
                     /translation="MSGFREFIAKGNVLDLAVAVIIGAAFATIVGSLTDDIIMPVIGW
                     LFGGLDFSSYFLRLGPIPSGFAGDPNNYAALKEAGVPMIGYGQFVTAVVAFLILAFVI
                     YQLVRMAARVKLAPAEAPPAGPSEELLVLREIRDSLKARP"
BASE COUNT          330 a          744 c          736 g          314 t
ORIGIN      
        1 cgcaagctcc gccgggctga cctcctcggg cccggtcagg accagaagcg gcccgttgcc
       61 gctccagtcc gcctcgaagg cgcgcttcac gtccgccggg gtcaggccgg cgaccagcag
      121 gtcgtagact cgcatcgcct ccctgggatg gagatagggg gtcccggcca gctcggcatc
      181 ggcgatcccc tgggcgagct cccgggtgcc tcgggtggcg ctctgataga cggcggcgcg
      241 cagccgcgag cgaagctgct cggcggcggt ctcgacttcc agcggggttg gtcccgtttc
      301 ggcgaaacgg cgcagctccg cctgcgcttc ggccagcccc tcccgccact tgccctggtt
      361 tggcaccgcg atcaggcagg cgacccgggc atcgggcagg ctcctgttga ccatcggcgc
      421 cgttccgaga agcgaggagc ccgtcctcgt ggtcgcctgg ctggcgcggt cggtgaggat
      481 cgtcacccag atctgggaca aggcgtcccg gcgcatccgc tccaggctcg aatcgcgcgg
      541 cccgtcgagc ggcgccaccc ggcaggcgct ctcggccagc ggcagcgaag cgccgctcct
      601 cgtaaaggcg tcgaggccgc gctggggcaa agaggctggc ttcgctcggg tccccggcgc
      661 gcctttcccg gtccagcttc cgaacgcggc ttgcgccgcc ttcagcaggg cgtccggatc
      721 ggcgtcgccg gcaatgacca gcacggcatt gtccggccgg taccagcggt cgtaaaaggc
      781 ctggagggcg gccggagcag ccgcccgaac cgactcctcg gtcccgccaa tgtcgcgggc
      841 gaacgagcgc agctccttcg cctggaaccg tgcggcttcg cgcccgacga tcgacgccgg
      901 gttcgaccgg gcgcggatct cggcgaccac gacgcccttc tccagctcga cggcggcggg
      961 cgcgaacagg attccgtcgg cggcgccgcg aagccattcg agcaccttcg agaccgccgc
     1021 cgggtcggcg gccgggaggt ccatctggta gacggtcgac tccaggcccg tcaccgcatt
     1081 ctcgtcgcgg ccgaaagcga ccccgaggcc ggcgaaacgg gtgtcgagcg agccttcggg
     1141 cgcctggcgg gtggagcgga acgccatatg ctcgatgaaa tgggcgaagc cgccctcggc
     1201 ttcctcctcg tcatagctgc cggcgcggat ggcgagtcgg atcgacaccg cccgggcggg
     1261 cgaggcgttg cgcatgatcg cgtagcgcag gccgttgggc agggtgccgg tcctgaccgc
     1321 cgggtccgga gtcgccttcg catcggcggg caacggatcg accgccgaag cggggccgct
     1381 cgcgaccagc aaagccgccg cggcgagcgc ccggccgaac caactcaact tcatcttatt
     1441 ccccgcccaa gtccgcgccg tcagctaacg gaaaaagctt aacttttcaa cggttcaagg
     1501 tcgtgccttg agcgaatccc gaatctcgcg caggaccaga agctcctcgc tcggcccggc
     1561 cggcggcgcc tctgcgggcg ccagcttcac ccgggccgcc atccggacca gctggtagat
     1621 gacgaaggcg aggatgagga aagcgaccac ggcggtgacg aactggccgt agccgatcat
     1681 cggcacgccc gcttccttga gcgcggcata attgttggga tctccggcaa agcccgaggg
     1741 gatcggcccg aggcggagga agtagctcga gaaatccagg ccgccgaaca gccagccgat
     1801 aaccggcatg atgatgtcgt cggtcagcga gccgacgatg gtcgcgaagg ccgcgccgat
     1861 gatcaccgcc accgccaggt cgagcacgtt gcctttggcg atgaattccc tgaatccgga
     1921 catatgcgcc tcccccttgt tgtgggggag ggtagcgcga ctgggctaca ctctcaaatc
     1981 ctccccggca tttgccgggg agggggaccg cgcgcagcgc ggtggagggg ttctgggcgc
     2041 ttgaagaacc cctccactag gcttcgcctg gtccccctcc cccgggaaat ccgggggagg
     2101 atttcggctc aatgcccatc gccc
//
LOCUS       DASWNU010000132         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_446057, whole genome shotgun sequence.
ACCESSION   DASWNU010000132 DASWNU010000000
VERSION     DASWNU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446057"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VGB57_02540"
     CDS             <1..776
                     /locus_tag="VGB57_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY0130256.1"
                     /translation="TRRRHGFLWQEHCILTARELLGESFTGTSNVYLAMKHGLEAKGT
                     NAHELPMVFAALARQRFPGDAEALRQSQYKVLQRWQNQYSGNLLVFLPDTYGTTQFLK
                     GAPQWVSYWTGARPDSKEPIAAGEELLAFWGRVGAMPETVAAKKLIIFADGLDVALPG
                     AEPNGADIPSIQRHFAGRVQAGFGWGTNFTNDFIGCDPQGAERMKPLSLVCKVAAVNG
                     DPAVKLSDNHAKATGPAEEVAVYRAVFGSEGIKDAPVKV"
     gene            787..1425
                     /locus_tag="VGB57_02545"
     CDS             787..1425
                     /locus_tag="VGB57_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEY0130257.1"
                     /translation="MNGFEAQPGDALIVIDPQLDFCPGGALAVAGGDEIMAGIAGLAS
                     RFELVVVTQDWHPKGHASFASSHEGSAPFGTTRLPYGEQVLWPDHCVQGSAGADFHPA
                     LAPALERAHLILRKGYHPGVDSYSAFYENDRTTRTGLAGYLRDKGIRRCVLVGLAYDF
                     CVAYSAVDAVREGFEAVVLKGLTRAIAMPGAGGGTTVDEAEAGFREVGVAVA"
     gene            complement(1560..1808)
                     /locus_tag="VGB57_02550"
     CDS             complement(1560..1808)
                     /locus_tag="VGB57_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEY0130258.1"
                     /translation="MSATDSIREHMEVIGADGVHVGTVDHVEGNRIKLTKADSGQGSH
                     EGHHHYISLGLVATVEGDQVRLSANADVAVQFEEEEGD"
     gene            complement(1823..2389)
                     /locus_tag="VGB57_02555"
     CDS             complement(1823..2389)
                     /locus_tag="VGB57_02555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY0130259.1"
                     /translation="MADLAILAFAGSLRRGSYNRALLRAAADLAPEGMRIEIFDLAEV
                     PLYNGDVEAEGDPEAVARLKRAIAAADGILMATPEYNHGVPAVMKNAVDWASRPPGSA
                     PLGGKPVGIVGASPGITGSARGQSQLRQAFEFTDSYCMAQPELLVFKAHEKFDPEGRL
                     TDGATRQHLARYLTAFAAWVRRFVPDQP"
     gene            complement(2389..4071)
                     /locus_tag="VGB57_02560"
     CDS             complement(2389..4071)
                     /locus_tag="VGB57_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0130260.1"
                     /translation="MVEPETPDSRTAQRYPSLAPAELERLGRFGTRRSYAPGEMLVRS
                     GESGHGLSLILSGEVDITRHARDGEAGQLIVTHGPGAFIGELAQLSGQPALVDAQARG
                     EVEAVVIPPERLRALLVAEAEVGERIMRALILRRVGLIETGTGGPILVGREGSADLLR
                     LEGYLSRNGHPHQRLDADKDFDARALVERFHVAPEELPIVLCPGGQLLRNPTEACLAR
                     CLGLVGPIDPDRVFDVAVIGAGPAGLATAVYAASEGLSVLALDCRAFGGQAGASARIE
                     NYLGFPTGISGMALMGRAFNQARKFGVEMAIPDEAVKLECDEGGGRRLGLANGERIRA
                     RSIVLATGARYRRLAVSNLEDFEGASVHYWASPLEAKLAAGQDVALVGGGNSAGQAVV
                     FLAGHARRVTLIARRPLEETMSAYLVERIASLPNVELVAGAEVSALEGRDRALDSICW
                     RSRGSDRETRRACRHLFLFIGAEPNTDWLQGSGLKLDPRGFILTGPEARAGCLPLETS
                     RPGVFAVGDVRAASVKRVAAAVGDGAQVVAALHTYLAQAPAAIVALPVPVSA"
     gene            4388..>4808
                     /gene="msrB"
                     /locus_tag="VGB57_02565"
     CDS             4388..>4808
                     /gene="msrB"
                     /locus_tag="VGB57_02565"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0130261.1"
                     /translation="MEPLASPRRDAVAARLTEEERRVILDQGTEPAFCGTLLNNKSAG
                     IYACRLCGLPLFKSERKFESGTGWPSFTRPFDPEHVREIEDRAYGMVRTEIRCARCDG
                     HLGHVFPDGPPPTGLRYCLNSVSLEFFEAEGEIEQRTN"
BASE COUNT          727 a         1653 c         1701 g          727 t
ORIGIN      
        1 gaacccgccg ccgccacggc ttcctgtggc aggagcattg catcctgacc gcccgcgagc
       61 tgctgggcga aagcttcacc ggcacctcca acgtctacct ggcgatgaag cacggcctcg
      121 aggccaaggg caccaacgcc cacgagcttc cgatggtctt cgccgccctc gcccgccagc
      181 gtttccccgg cgacgcggag gcgcttcgcc agtcgcaata caaggtgctc cagcgctggc
      241 agaaccagta tagcggcaac ctcctcgtct tcctccccga cacctacggc accacccagt
      301 tcctgaaggg cgcgccgcaa tgggtgagct actggaccgg ggcccgcccc gattcgaagg
      361 agccgatcgc cgcgggggag gagctgctcg ccttctgggg ccgggtcggc gcgatgccgg
      421 aaacggtggc ggcgaagaag ctgatcatct tcgccgacgg cctcgacgtg gcgcttccgg
      481 gcgcggagcc gaacggcgcc gacatcccct cgatccagcg ccacttcgcc ggccgggtgc
      541 aggccggctt cggctggggg accaatttca ccaacgactt catcggctgc gatccgcaag
      601 gggcggagcg gatgaagccg ctctccctcg tctgcaaggt ggcggcggtg aacggcgatc
      661 cggcggtgaa gctcagcgac aaccatgcca aggcgaccgg cccggcggag gaggtcgccg
      721 tctatcgcgc cgtgttcgga agcgagggca tcaaggacgc cccggtcaag gtgtgaggag
      781 gacgagttga acggcttcga agcgcaaccg ggcgacgccc tgatcgtgat cgatccgcag
      841 ttggatttct gccccggggg agccctcgcc gtcgccggcg gcgacgagat catggccggg
      901 atcgccgggc tcgcctcgcg cttcgagctg gtggtggtga cccaggactg gcacccgaag
      961 ggccacgcct ccttcgccag cagccatgag ggctcggcgc cgttcggaac gacccggctg
     1021 ccctatggcg agcaggtgct gtggccggac cattgcgtcc agggcagcgc gggggcggac
     1081 ttccacccgg cgctggcgcc ggcgctggag cgggcccatc tcatcctccg caagggatac
     1141 cacccgggcg tggattccta ttcggctttc tacgagaacg accgcacgac ccgcaccggc
     1201 ctcgccggct atcttcgcga caagggcatt cggcgctgcg tcctggtcgg cttggcctac
     1261 gatttctgcg tcgcttattc ggcagtggac gcggtccgcg agggcttcga ggcggtcgtg
     1321 ctcaagggcc tcacccgggc catcgccatg cccggagcgg gcggcggaac cacggtcgac
     1381 gaggcggaag ccggtttccg tgaggtgggc gtggcggtgg cctgaattcc tcccccggat
     1441 ttccggggga gggggaccgc gcggagcgcg gtggaggggt tcttcggatt cagcaaagcc
     1501 cctccaccag gcttcgcctg gtccccctcc cctgcaaatg caggggagga tttacgcgct
     1561 cagtcgccct cttcttcctc gaactggacg gcgacgtcgg cgtttgcgga gaggcgcacc
     1621 tggtccccct cgacggtcgc gacgaggccg agcgagatgt aatggtggtg gccttcgtgc
     1681 gagccctggc cgctgtcggc cttggtgagc ttgatccggt tgccctcgac atggtcgacg
     1741 gtgccgacgt ggacgccgtc ggcgccgatc acttccatat gttctctgat cgagtcggtt
     1801 gcgctcattc gaggctcctt tttcagggtt ggtcggggac gaaacgccgc acccaggcgg
     1861 cgaaggcggt gaggtaacgg gccagatgct ggcgggttgc cccgtcggtc aacctgcctt
     1921 ccggatcgaa cttctcgtgc gccttgaaga cgagcaattc gggctgggcc atgcagtagc
     1981 tgtcggtgaa ctcgaacgcc tggcggagct ggctctggcc gcgggcggag ccggtgatcc
     2041 cgggcgaggc gccgacgatc ccgaccggct tgccgcccag cggagcggat ccgggcgggc
     2101 gcgacgccca gtcgaccgca ttcttcatca ccgccgggac gccgtgatta tattccggag
     2161 tcgccatcag gatcccgtcg gcggcggcga tcgcccgctt gaggcgggcg acggcctccg
     2221 gatcgccctc ggcctcgacg tcgccattgt agagcgggac ttcggcgagg tcgaaaatct
     2281 cgatccgcat tccctccgga gcgagatcgg cggccgcccg aagcagggcg cggttgtagg
     2341 agccgcggcg aaggcttccg gcgaaggcga ggatcgcgag gtcggccatc aggcggagac
     2401 cggcacgggc aaggcgacga tcgcggccgg ggcctgcgcc agataggtgt ggagcgcggc
     2461 caccacctgg gcgccgtcgc cgaccgccgc ggcgacccgc ttgaccgagg cggcgcggac
     2521 gtcgccgacg gcgaagaccc ccggccggct ggtctcgagc ggaaggcagc cggcccgggc
     2581 ctcgggcccg gtgaggatga agccgcgcgg gtcgagcttg agtccggagc cctggagcca
     2641 gtcggtattg ggctcggcgc cgatgaacag gaacaggtgg cggcaggcgc ggcgggtctc
     2701 ccggtccgag ccgcgcgacc gccagcagat cgaatcgagt gcccggtcac ggccttccag
     2761 cgcggagact tcggcgccgg cgaccagctc gacattgggc aggctggcga tcctctcgac
     2821 cagataggcg gacatcgtct cctcgagcgg gcggcgcgcg atcagggtga cccggcgggc
     2881 gtggccggcg aggaagacca ccgcctggcc ggccgagttg ccgccgccga ccagcgcgac
     2941 gtcctggccg gcggcgagct tcgcctccag cggcgaggcc caataatgga ctgaggcgcc
     3001 ctcgaaatcc tcgaggttgg agaccgcgag gcggcgatag cgggcgccgg tggcaaggac
     3061 gatggaccgg gcccggatcc gttcgccatt ggccaggccc aggcggcgcc cgccgccctc
     3121 gtcgcattcg agcttcaccg cctcgtccgg gatcgccatc tcgactccga atttccgcgc
     3181 ctggttgaag gctcgtccca tcagggccat gcccgagatt ccggtcggga agccgagata
     3241 attctcgatc cgagccgagg ctccggcctg cccgccaaag gcccggcagt ccagcgccag
     3301 cacgctcagc ccttcggagg cggcgtagac cgcggtggcg agcccggccg gcccggcgcc
     3361 gatcacggcg acgtcgaaga cccggtccgg atcgatcggg ccaaccaggc cgagacagcg
     3421 ggcgaggcag gcctcggtcg ggttgcggag caattggccg ccggggcaga gcacgatggg
     3481 aagctcttcc ggagcgacgt ggaagcgctc gaccagcgcc cgggcgtcga agtccttgtc
     3541 ggcgtccagc ctctggtggg ggtggccgtt gcgcgagaga tagccttcga gccgaagcaa
     3601 atcggcactt ccctcgcggc cgaccagtat cggcccgccg gtgccggtct cgatcagccc
     3661 gacgcgcctg aggatgaggg cgcgcatgat ccgttcgccg acctcggctt ccgcgaccag
     3721 aagcgcccgc agccgctccg gcgggatcac caccgcctcc acctcgcccc gcgcctgggc
     3781 gtcgaccagc gccggctggc ccgataattg ggcgagctcg ccgatgaacg cgcccggccc
     3841 gtgggtgacg atgagctggc cggcttcgcc gtcgcgggcg tgccgggtga tgtcgacctc
     3901 gcccgacagg atcaggctga ggccgtggcc gctctccccg ctgcgcacca gcatctcgcc
     3961 gggcgcgtag ctgcgccggg tgccgaagcg gccgaggcgt tccagctccg cgggcgcgag
     4021 cgaaggatat ctttgcgcgg tccggctgtc cggggtctcg ggttcgacca ttccccggct
     4081 ttaggccgtg gcgacgggca aggcgagagg cggtgcgcgg tggcgtcatg atgaacctgt
     4141 tccagcatcc agtctgcgtc tccgaggggg tcgaccgccc cgcgatttgc gctatccgtc
     4201 acgctggcga aagccggggc ctcggggagg cccgccgtgg ttctccgacc cgggatcccg
     4261 ccttccgccg ggatgacgac ccgcactgac cattgtaacg cacctcttcg agcccgacct
     4321 tggcctgaag acgcttcgaa ggaattcccg gatgaccacc cccgtcacct cccgctcagg
     4381 ctacgacatc gagccgctcg ccagcccccg ccgcgacgcc gtcgccgccc ggctgaccga
     4441 agaggagcgg cgggtgatcc tcgaccaggg caccgagccc gctttctgtg gcacactgct
     4501 gaacaacaag tcggcgggaa tctatgcctg ccggctgtgc ggcctgcccc tgttcaaatc
     4561 ggagaggaag ttcgagagcg gcaccggctg gccgagcttc acccgtccgt tcgatccgga
     4621 gcatgtccgc gaaatcgagg accgcgccta cggaatggtc cggaccgaga ttcgctgcgc
     4681 ccgctgcgac ggccatctcg gccacgtctt ccccgacggc ccgccgccga ccgggcttcg
     4741 ctactgcctg aactcggtat cgctggagtt cttcgaggcg gagggggaaa tcgagcagcg
     4801 gacgaatg
//
LOCUS       DASWNU010000133         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_393739, whole genome shotgun sequence.
ACCESSION   DASWNU010000133 DASWNU010000000
VERSION     DASWNU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393739"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VGB57_02570"
     CDS             <1..464
                     /locus_tag="VGB57_02570"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130262.1"
                     /translation="LYILVAARAAEAIVGPATPIVEMTGHRLLPLVNSLVGVAAWAGL
                     SYMLVPLYGAAGMAVAVGAATVAIAYAATLELKLAEGMSPFDMKLFRGLFVALLGVAL
                     MALAAYWTRGPLRFASVLSLWAAASWCALRFGLVRGDREALGGLARRLRLI"
     gene            728..1474
                     /locus_tag="VGB57_02575"
     CDS             728..1474
                     /locus_tag="VGB57_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0130263.1"
                     /translation="MNFRELVVAYFQYPAIIAYLALSAAAIAFYAARPAPLAPTLIAV
                     AVATLIYPFVWYMLHRFVLHSKWMWKSKLLAPTWKRIHYDHHQDPNHLEVLFGALHTT
                     LPTIAVATLPIGYAIGSPWGSQAAWGAAAAAFATGLVTTCFYEFVHCIQHLAYKPKRK
                     WLAEMKKRHMAHHFHDENGNYGITSFLWDRAFGTFYDRPERPKKSPTVFNLGYTPEVA
                     EVYPHVAKLSGGVATDHPRERVRQKAGLGN"
BASE COUNT          317 a          665 c          604 g          331 t
ORIGIN      
        1 tcctctacat cctcgtcgcc gcccgggcgg cggaagcgat cgtcggcccg gccaccccga
       61 tcgtcgagat gaccggccac cgcctcctgc ctctcgtcaa cagcctcgtc ggagtggcgg
      121 cctgggcggg gctgagctac atgctggtgc cgctctacgg cgccgccgga atggcggtcg
      181 cggtcggcgc cgcaaccgtc gccatcgcct atgccgccac cctcgagttg aagctcgccg
      241 aggggatgag cccgttcgac atgaagctgt tccggggcct gttcgtcgcc ctcctcggag
      301 tggccctgat ggcgctcgcc gcttactgga cccgcggccc gcttcgcttc gcctcggtcc
      361 tgtcgctgtg ggcggcggcg agctggtgcg cgctccgctt cggcctggtc cggggcgacc
      421 gcgaggcgct cggcggtctg gcgcgccgac ttcgactgat atgacgtcat cccggcgcaa
      481 gccgggacct cggggagacc cgccgagact tctcgtcccg gggtcccggc ttgcgccggg
      541 atgacgtgga gcgccaaaag ccctaaaagc gccgcattgc cattgcaggg atcggcccca
      601 tattggcgag ccgcgcgttg gcggcggcca accgtggcgc tggtgcgaaa ccgaggaatc
      661 gaacgacgtg aagcccgctt tgacgatccg cccgagcttc tggaggcgag agcatcatct
      721 cgacaagatg aatttccgcg agctggtcgt ggcctatttc caatatcccg cgatcatcgc
      781 ctacctggcg ctgtccgcgg cggcgatcgc cttctacgcc gcccgcccgg cgccgctcgc
      841 cccgaccctg atcgcggtcg cggtcgcgac cctcatctac ccgttcgtct ggtacatgct
      901 ccaccgcttc gtccttcaca gcaagtggat gtggaagtcg aagctcctcg ccccgacctg
      961 gaagcgcatc cattacgacc atcaccagga tcccaaccac ctcgaggtcc tgttcggagc
     1021 tctccacacc acgctcccga cgatcgccgt cgccaccctc cccatcggct acgcgatcgg
     1081 ctcgccctgg ggctcgcagg ccgcctgggg cgccgccgcc gccgccttcg cgacgggcct
     1141 cgtcaccacc tgcttctacg agttcgtcca ctgcatccag cacctcgcct acaagcccaa
     1201 gcgcaaatgg ctggcggaaa tgaagaagcg ccacatggcc caccatttcc acgacgagaa
     1261 cggcaattac gggatcacga gcttcctgtg ggaccgggct ttcgggactt tctacgaccg
     1321 gccggagcgg ccgaagaaga gcccgacggt tttcaacctt gggtatacgc cggaggtggc
     1381 ggaggtttat ccgcatgtgg cgaagcttag cgggggcgtc gccaccgacc atccgcggga
     1441 gcgcgtgcgg cagaaggctg gcttgggcaa ttgagctccc gcgggcacta aaagtaacca
     1501 cgaagaccca gtgttgaggc gtcggcgggc gtgcgtcttg ctttgtgccg ctcggagcgg
     1561 atttcaccgc cggccgcggg cgccgcaaac tgcagtccca ggcgcaagcg gcttgcggaa
     1621 atgaagaagc gccacatggc caccatttcc acgacgagaa cggcaatcag ggccgttcga
     1681 caggaatagc gcgccgctaa gatcctgcgg gaccgctggg gccagcgctg ggacgttgtc
     1741 gcggtcgtac ttttctatgg gcagccttgg agtggctcga taacaaccca attgcgccat
     1801 ccctcggccg cagcatcgga taatgggctt ttcagccgca ccggagacgt ggaaaatgcc
     1861 cactggaact cacgagcaga cgatcaacac tgctctgggc gaggtgcttc acgacct
//
LOCUS       DASWNU010000134         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_187222, whole genome shotgun sequence.
ACCESSION   DASWNU010000134 DASWNU010000000
VERSION     DASWNU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187222"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGB57_02580"
     CDS             complement(<1..181)
                     /locus_tag="VGB57_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130264.1"
                     /translation="MRKFTALAAVAATLTVGGIAVAKEKKADAPGAVKEKKICRGEAQ
                     SYSRIPRKKVCRTQAE"
     gene            complement(212..448)
                     /locus_tag="VGB57_02585"
     CDS             complement(212..448)
                     /locus_tag="VGB57_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130265.1"
                     /translation="MRNFNMLAAISAALLVSVGVSAKEKPDQPKPRKVCRTEQMSGRI
                     TPQRICRVVRPSDVSTQENQRKAGDARAAGSDRN"
     gene            653..1120
                     /locus_tag="VGB57_02590"
     CDS             653..1120
                     /locus_tag="VGB57_02590"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY0130266.1"
                     /translation="MNDLPYRPAAGVMLVNAEGKVWVGQRLDSSLEAWQMPQGGLDEG
                     EDPEQGALRELEEETGIRPDLVEIVARHGEELYYDLPGELVARLWKGRWRGQRQTWFL
                     ARFLGTDDDVDIATPHPEFRAWKWADPADLPAMIVPFKKKLYEDVLEAFRDRL"
     gene            1241..1456
                     /locus_tag="VGB57_02595"
     CDS             1241..1456
                     /locus_tag="VGB57_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130267.1"
                     /translation="MTDLPVTEVRDMFSHADCVPPFAAVPPNSDNGAATTAPFFHFMG
                     SRNNEPCVSLMSLFGQPSVVTSPFLVG"
     gene            1467..2678
                     /locus_tag="VGB57_02600"
     CDS             1467..2678
                     /locus_tag="VGB57_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY0130268.1"
                     /translation="MRGLTKDEAAAVERVAGEPMLAQVEAWAAVNSGSHNLAGLNQVA
                     ALLADAFAALPGEPVLREAGPVDSITPSGERLALRHGRNLHLAVRPRAPVQLLFTGHM
                     DTVFAADHPFQDVFWRDEGVLGGPGVADMKGGLAVLLAALKAVEASPFAAGLGYEVVI
                     NSDEEVGSPGSAALIAEAARGKKAALTYEPSALPDGTLAGARPGSGNFSLIIKGRSAH
                     AGRNPEEGRNAVLAAAELALRLADAKAEGLSVNPARIDGGGPNNVVPDHAVLRINMRP
                     RTLADQARAQAALEEAVGQVAAKRDLCIIMHGGWGRPPKPLDPAAERLFALVASCGAD
                     LGQKIGWRDSGGVCDGNNIAACGVPVVDTMGVRGGAIHSDAEYLIVESLAERARLSAL
                     TILRLAQGEGL"
     gene            2675..>3444
                     /locus_tag="VGB57_02605"
     CDS             2675..>3444
                     /locus_tag="VGB57_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEY0130269.1"
                     /translation="MSFRIRPARNDDFQALYEMAKLTGGGFTNLPADRGALVEKLIRS
                     EKGFAAELTEPGDELYVFMLENVETGQIRGTCQIFGMVGRDAPFYSYRISTLTQTSKA
                     LGQTFRNEMVTLTTDLEGSSEVGGLFLHPGERAGGLGMLLARSRYLFIKLHRERFARR
                     VIAELRGVIDEGGGSPFWDSIAGRFFGMSFQEADEFNGAHGTQFIADLMPKTPIYTAM
                     LPEHARAVMGVPHPSGRAAMKMLEREGFHFDCYVDIFDG"
BASE COUNT          560 a         1170 c         1145 g          569 t
ORIGIN      
        1 attcggcctg cgtgcggcag accttcttcc gcggaatccg gctgtaggac tgggcttcgc
       61 ccctgcagat cttcttttcc ttgaccgctc cgggcgcgtc ggccttcttc tccttggcga
      121 cggcgattcc gccgaccgtc agggtggcgg cgaccgccgc cagtgcagtg aatttgcgca
      181 tcttactctc cgttctcgaa ttcgtggtcg ctcagttccg gtcgctcccg gccgctcggg
      241 cgtcgccggc cttgcgctga ttttcctggg tcgacacgtc cgaggggcgc acgacccggc
      301 aaatgcgctg gggcgtgatt cggcccgaca tctgctcggt gcgacagacc ttcctgggct
      361 tgggctgatc cggcttttcc ttcgccgaaa cgccgacgga caccagcaag gcggccgata
      421 tggccgccag catgttgaaa ttacgcattt tccctccggt cccagagggc gagcaatgtc
      481 cgaaatggat gcaaaagtca accaagctaa gcagttagcg ccgattcaag caatatcggt
      541 ggccccggtt ggcggacggc cgcttgcgca ataaagggag tggctcgcgt ccatcacccg
      601 ccggggcccc ggccttcgcc gggacgacgg cccggacaat gacgaggcag ccatgaacga
      661 cctcccctac cgccccgccg ccggcgtcat gctggtcaac gccgagggca aggtgtgggt
      721 cgggcagcgc ctcgatagca gtctcgaggc gtggcagatg ccgcagggcg gcctcgacga
      781 aggcgaggat ccggagcaag gcgcgcttcg ggagcttgaa gaggaaaccg gaatccgacc
      841 cgatctggtc gagatcgtcg cccggcacgg cgaggagctc tattacgacc tgcccgggga
      901 gctggtcgcc aggctgtgga aaggccgctg gcgcggccag cgccagacct ggttcctcgc
      961 ccgcttcctc ggcaccgacg acgacgtcga catcgccacc ccccatcccg aattccgggc
     1021 ctggaaatgg gccgaccccg ccgacctgcc ggcgatgatc gtgcccttca agaagaagct
     1081 ctacgaggac gtgctggagg cgttccgcga ccggctgtga ggccggccgg aggacaagct
     1141 cggcggagcg ccacccacga acgtcaccct gaactcgatt cgcaggtcca gcttcgagcc
     1201 tcgcgacctt catcgagctg gatgctgaag caagttcagc atgacggact tgcccgttac
     1261 cgaagttcgt gatatgttct cgcatgccga ttgcgtcccc cccttcgccg cggtgccccc
     1321 gaacagcgac aacggcgccg ccacgacggc tccctttttc cacttcatgg gaagccgaaa
     1381 caacgaacct tgcgtgtcgc tcatgtcgct gttcggccag ccctcggtgg tgacatcgcc
     1441 gttcctcgtc ggctaaggct tcgccgatgc ggggccttac caaagacgaa gcggcggcgg
     1501 ttgagcgtgt cgccggggag ccgatgctgg cgcaggtcga ggcctgggcg gcggtgaaca
     1561 gcgggtcgca caatctggcc gggctgaacc aggtcgccgc ccttctcgcc gacgccttcg
     1621 ccgcgcttcc gggcgagccg gtccttcgcg aggccgggcc agtcgattcg atcaccccct
     1681 cgggcgagcg gctcgccctc cggcacggcc gcaacctcca tctcgccgtc cggccgcggg
     1741 cgccggtgca attgctcttc accggccaca tggacacggt gttcgcggcc gatcacccgt
     1801 tccaggacgt cttctggcgc gacgaaggcg tgctcggagg gcctggagtc gccgacatga
     1861 agggcgggct cgccgtcctt ctcgcggcgc tgaaggcggt cgaagcctcg ccgttcgccg
     1921 ccgggctggg ctacgaagtg gtgatcaact cggacgagga ggtcggctcg cccggctcgg
     1981 ccgcgctgat cgccgaggcg gcgcgcggca agaaggccgc gctcacctac gagccttccg
     2041 ctctgcccga cggaacgctg gccggggcga ggcccggcag cggcaatttc tcgctgatca
     2101 tcaagggccg aagcgcccat gccggccgca atcccgagga aggacgcaat gcggtgctgg
     2161 cggccgccga gctggcgctc cgtctcgccg acgcgaaagc cgaggggctg agcgtcaacc
     2221 cggccaggat cgacggcggc gggcccaaca acgtcgttcc cgaccatgcg gtgctgcgca
     2281 taaatatgcg tcccaggacc cttgccgacc aggcgcgggc gcaggcggcg ctggaggagg
     2341 cggtggggca agtggccgcg aagcgcgacc tgtgcataat tatgcacggc ggctgggggc
     2401 gtccgcccaa gccgcttgat ccggcggccg aaagattgtt cgccctggtc gcgagctgcg
     2461 gcgccgacct cggccagaag atcggctggc gcgattcggg cggagtgtgc gacggcaaca
     2521 atatcgccgc ctgcggagtg ccggtggtcg acacgatggg cgttcgcggc ggcgccatac
     2581 attcggacgc cgaatatctg atcgtcgaga gcctggccga acgcgcgcgc ctgtccgcgc
     2641 tgacgatcct gaggctggcg cagggggagg gtttgtgagc tttcggatcc ggcccgcccg
     2701 caacgacgat ttccaggcgc tttacgaaat ggcgaagctg accgggggcg gcttcaccaa
     2761 cctccccgcc gaccgcggag cgctcgtcga gaagctgatc cgctccgaga aaggtttcgc
     2821 cgcggagctc acggagcccg gcgacgaact ctacgtcttc atgctcgaga atgtggagac
     2881 ggggcagatc cgcggcacct gtcagatttt cggaatggtg ggccgcgacg cccctttcta
     2941 cagctaccgg atcagcaccc tgacccagac ctccaaggcg ctcggccaga ccttccgaaa
     3001 cgagatggtg accctgacca ccgacctcga aggctcgtcc gaggtcggag gcctgttcct
     3061 gcacccgggc gagcgcgccg ggggcctcgg catgctgctc gcgcgcagcc gctatctgtt
     3121 catcaagctc caccgcgagc gcttcgccag gcgggtcatc gccgagcttc gcggagtcat
     3181 cgacgaaggc ggcggatcgc ctttctggga ttcgatcgcc ggccgcttct tcggaatgag
     3241 cttccaggag gcggacgagt tcaacggcgc ccacggcacc cagttcatcg ccgacctgat
     3301 gcccaagacg cccatctaca cggcgatgct ccccgagcac gcacgcgccg tgatgggcgt
     3361 cccccaccct tcgggacgcg cggcgatgaa gatgctcgag cgcgagggct tccacttcga
     3421 ctgctatgtc gacattttcg acgg
//
LOCUS       DASWNU010000135         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_56841, whole genome shotgun sequence.
ACCESSION   DASWNU010000135 DASWNU010000000
VERSION     DASWNU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56841"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..847
                     /gene="rplJ"
                     /locus_tag="VGB57_02610"
     CDS             332..847
                     /gene="rplJ"
                     /locus_tag="VGB57_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY0130270.1"
                     /translation="MDRAQKAEQAAQLKRTFSETAVVVVTRNLGLTVAQSTTLRNRMR
                     DVGATFKVTKNTLTLIALEDTTYAPISTMLTGPTALATSADPVSAAKVAVDFAKTTDK
                     FEIVGGAMGDTVLDASGVRALAELPSLDELRATIVGLIQAPATKIARTISEPGAMLAR
                     VFGAYAAKEDA"
     gene            973..1344
                     /gene="rplL"
                     /locus_tag="VGB57_02615"
     CDS             973..1344
                     /gene="rplL"
                     /locus_tag="VGB57_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY0130271.1"
                     /translation="MADINSIVDQLSELTVLEAAELAKALEERWGLSAAAAVAAAPAA
                     AAAGPVEEEKTEFDVILTGDGGKKINVIKEVRAITGLGLGEAKALVEGAPTAVKVGIS
                     KAAAEELKKRIEEAGGTVELK"
     gene            1470..>1765
                     /locus_tag="VGB57_02620"
     CDS             1470..>1765
                     /locus_tag="VGB57_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130272.1"
                     /translation="MAKSATDVMQDIVFAAVIDSIVALKAASKGVPNTLLRDINAVHV
                     NASFADLPKELQAAITANVRSAFNRLLKEGYSVSPGRPEPQRRPEPPRRPPSGGA"
BASE COUNT          296 a          600 c          599 g          270 t
ORIGIN      
        1 tgaggcagcg gcccgaggga tgcggccgaa gcgcttgatc cccaacggct atgcccctat
       61 aggcgccgct tcgcacgcac cgggctcgcc cggcgcgcgc gtccgtccga gacagccggt
      121 gggggcatcg ctcccttaat cttccggccg agacggggaa agagtttcac cgccgccctt
      181 cgcgggcgtc cggtctgccg aagtctttca caggctcttc ggctgcgctc ccctcccctt
      241 cggacactgg ccggctttgc gggttcgcgc ccgtgcgccg gtcgaaccgc ccgccggatg
      301 gtccggcggg tcgtttggag tgcaaggcaa tatggatcga gctcagaaag ccgagcaggc
      361 cgcccagctg aagcgcacct tttccgagac ggccgtggtt gtggtcaccc gcaacctcgg
      421 cctgacggtg gcccagtcca ccacgcttcg gaatcggatg cgcgacgtcg gcgcgacctt
      481 caaggtgacc aagaacacgc tcaccctgat tgcgctcgag gacacgacct acgctccgat
      541 cagcaccatg ctgaccgggc ccaccgccct tgccacgtcg gccgatccgg tctccgcggc
      601 gaaggtagcg gtggatttcg ccaagacgac cgacaagttc gagatcgtcg gcggcgcgat
      661 gggcgatacg gtcctcgacg cgagcggcgt gagggcgctt gccgagcttc cgtctctcga
      721 tgaactgcgt gctacgatcg tgggcctcat tcaggcgccc gcgaccaaga tcgcgcggac
      781 catcagcgag ccgggcgcga tgctggcccg tgtcttcggc gcctatgcgg ccaaagaaga
      841 cgcctgaatt ttcaactgac acgtcgcccc ggcgcaggcc gggggcccag tgggattagc
      901 cgcttgcgcg aacccccgcc gggatcccgg ccttcgccgg gatgacaatt caacatcaac
      961 ggagactttg aaatggcaga catcaattcc atcgtcgatc agctcagcga gctgaccgtc
     1021 ctcgaagccg ccgagctcgc caaggcgctc gaagagcgtt ggggcctatc ggccgccgcc
     1081 gcggttgcgg ccgctccggc cgctgcggct gccggcccgg tcgaggaaga gaagaccgag
     1141 ttcgacgtca tcctcaccgg cgacggcggc aagaagatca acgtgatcaa ggaagtccgc
     1201 gcgatcaccg gcctgggcct gggcgaagcc aaggcgctgg tcgagggcgc cccgacggcg
     1261 gtcaaggtgg gcatctccaa ggccgcggcc gaggagctca agaagcgcat cgaggaagcc
     1321 ggcggcaccg tcgagctgaa gtaatccggc cgcgcccgag ggcgcaccgg tttgcgaccg
     1381 atcgaggggc ggctcccgtg cgggggccgc cccttttcgt ttggaagcgg cgcaggcagg
     1441 ctctaccctg cggcgcgaag ggaagcatga tggcgaagtc ggcgacggac gtgatgcagg
     1501 acatcgtctt tgcggcggtg atcgattcga tcgtcgcgct gaaggccgcg tcgaagggcg
     1561 tgccgaacac gctgcttcgc gacatcaacg cggtccatgt gaatgcgagc ttcgctgacc
     1621 tgccgaagga gctgcaggcg gcgatcaccg ccaacgtccg ctccgccttc aatcgcctcc
     1681 tgaaggaagg ctattcggtc tcccccggcc ggccggagcc gcagcgccgg cccgagccgc
     1741 cgcgcaggcc gccgtcgggc ggggc
//
LOCUS       DASWNU010000136         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_238956, whole genome shotgun sequence.
ACCESSION   DASWNU010000136 DASWNU010000000
VERSION     DASWNU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238956"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VGB57_02625"
     CDS             complement(<1..382)
                     /locus_tag="VGB57_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130273.1"
                     /translation="MNRKLASMGRLALRALGLSGLAAGLAMAFAAPANAQRVKVVPYL
                     EVQQVLSADLNGGDVLTYSTISAGVDASATTRRVQAQVSYRYERRIAWEDNVANDDVH
                     TGLAAVRAELVPNALAINAGALATR"
     gene            complement(379..1428)
                     /locus_tag="VGB57_02630"
     CDS             complement(379..1428)
                     /locus_tag="VGB57_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0130274.1"
                     /translation="MNQHSSLRTSGSLLERAADMYGFGSGLNAPLPPLPEAVEAEPAA
                     APAPEEVPVSEEPARPRRRKQPIILSSPEITFEEPAAPLELAPQDEAVEFMEEPKPAP
                     RPRSARRGSVDRASLRQGGFILPDAPVSELAEEFRIVKRQLLQAASGKTGIPEDKRQT
                     ILVCSASPDEGKTFCAINLALSLASEKEVEVLLVDGDFSKPEILSILGVEGGPGLIDA
                     IADPDSDPESFVIQTDVERFSVLPAGSQTNNVTELLASKRTRQVLDALTMRHPKRIVI
                     FDSPPALMASPASILANKVGQIVMVVRADKTNEADLREAVGLLSGCEHLALMLNGTGF
                     TPTGRRFGSYYGYSQ"
     gene            complement(1439..>2049)
                     /locus_tag="VGB57_02635"
     CDS             complement(1439..>2049)
                     /locus_tag="VGB57_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY0130275.1"
                     /translation="APVSTPNPAYTSLRAMAAERESVIAASMARKSQIQAAMTDLASN
                     QASEPVFAEQARLNRDYEVLKRQYEKLLEDREQVRLRGDIQTKTDAVQVKVIDPPSKP
                     TVPAAPNRPLFLTLILIVAAGAGVGAAFAKGQLQTTFPTQGRLEQATGLPVLGSISEV
                     VTPELKAQKQQRTKWFAGAGGALAASYAILMLVEFWQRSTVA"
BASE COUNT          312 a          651 c          732 g          354 t
ORIGIN      
        1 cccgggtcgc gagcgcgccg gcgttgatgg ccaaggcgtt ggggacgagc tccgcccgca
       61 cggcggcaag gccggtatgg acgtcgtcgt tggcgacatt gtcttcccag gcgatgcggc
      121 gctcgtagcg gtagctgacc tgcgcctgga cgcggcgggt cgtggcgctg gcgtcgaccc
      181 cggccgagat cgtcgaatag gtgaggacgt ccccgccgtt aaggtcggcg ctcaagacct
      241 gctggacctc cagatagggg accaccttca cgcgctgggc attggccgga gcggcgaaag
      301 ccatggcaag gccggcggcg aggccggaca ggccgagcgc gcgaagcgcc agccttccca
      361 tcgaggcgag cttcctgttc attggctgta tccgtaatag ctgccgaagc gccggccggt
      421 cggggtgaac ccggtgccgt tcagcatcag ggcgagatgc tcgcagcctg aaagcaggcc
      481 gaccgcttcg cggagatcgg cttcgttggt cttgtcggcc cgcaccacca tcacgatctg
      541 gccgaccttg ttggcgagga tcgaggcggg agaggccatc agcgcgggcg gagagtcgaa
      601 gatcacgatg cgcttgggat gacgcatcgt cagcgcatcg agcacttgcc tcgtcctctt
      661 ggaggcgagc agctcggtca cattattcgt ctggctgccg gcggggagca ccgagaagcg
      721 ctccacatcc gtctggatta caaaggattc cggatcgctg tccggatcgg cgatggcatc
      781 gatcaggccg gggccgccct cgacgccgag gatcgagagg atctccggct tcgaaaagtc
      841 gccgtcgacg agcaggacct cgacctcctt ctcgcttgcg agcgagaggg cgagattgat
      901 cgcgcagaag gtcttgccct cgtcgggcga ggccgagcag acgaggatcg tctgcctctt
      961 atcctcggga atgccggtct tgccgctcgc cgcctggagc agctgccgct tcacgatccg
     1021 gaattcttcg gcaagctcgc tcaccggcgc atcggggagg atgaacccgc cctggcgtag
     1081 gctggcgcgg tcgacgctgc cgcgacgggc cgagcgggga cgcggggccg gcttcggctc
     1141 ctccatgaac tcgaccgctt cgtcctgcgg agcaagttcg agcggagcgg cgggctcctc
     1201 gaaggtgatc tccggcgagg aaaggatgat cggctgcttg cggcggcgcg gccgggccgg
     1261 ctcttcgctt acgggaactt cttccggggc gggcgccgcg gccggttccg cctcgaccgc
     1321 ttccggcagc ggcggcaggg gcgcgttcag gcccgagccg aagccgtaca tgtcggccgc
     1381 ccgctcgagg agcgatccgc tggtcctgag gctgctatgc tggttcattt atggctcctc
     1441 aggcaaccgt gctgcgctgc cagaattcga cgagcatcag gatggcgtag ctggccgcga
     1501 gagcgccgcc ggcgccggca aaccacttgg tccgttgctg cttctgtgcc ttgagctccg
     1561 gcgtcacgac ttcgctgatc gagccgagca ccggcaagcc ggtcgcctgc tcgagccgcc
     1621 cctgggtcgg gaaggtcgtc tggagctggc ccttggcgaa ggcagcgccc acgccggcgc
     1681 ctgcggcgac gatcaggatc agggtcagga ataggggccg gttcggcgct gccggcaccg
     1741 tcggcttcga cgggggatcg atcaccttca cctggacggc atcggtcttg gtctgaatgt
     1801 cgcccctgag gcggacctgc tcgcggtcct ccagcagctt ctcatattgc cgcttcagaa
     1861 cctcataatc gcggttgagg cgggcctgct cggcgaagac cggttcggaa gcctggttgg
     1921 aggcaagatc ggtcatggcc gcttggatct ggctcttgcg ggccatcgag gcggcaatca
     1981 ccgactccct ctccgcggcc atggcacgaa gcgaggtgta ggcggggttg ggcgtgctga
     2041 ccggcgcga
//
LOCUS       DASWNU010000137         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_342231, whole genome shotgun sequence.
ACCESSION   DASWNU010000137 DASWNU010000000
VERSION     DASWNU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342231"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VGB57_02640"
     CDS             complement(<1..730)
                     /locus_tag="VGB57_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0130276.1"
                     /translation="MRRILQRLLLIAALAGAALLLFCYGTAVWEPKVRTIRVAAAGWP
                     SGTLPLRLVLISDVHVGGPDMPPARVRRIVEQINRLRPDIVLIAGDLVTDKRLATRYY
                     SHDEAVAPLAGLRPRLATIAVLGNHDHWRDAEAARRALARAGIRLLENDAVQVGPVAV
                     GGLDDDFTDRADLPATLAALKGLKGPRLILSHSPDPFADPAPGTFLMLAGHTHCGQVA
                     PPLIGPLSTMSDYGHRYACGVVREG"
     gene            complement(735..1019)
                     /locus_tag="VGB57_02645"
     CDS             complement(735..1019)
                     /locus_tag="VGB57_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0130277.1"
                     /translation="MSEGEAPPCFLCGRPLGTTVEWHHSVPKSRGGRETVPVHPVCHR
                     AIHRALDNKALARDYASAAALRSHPELARFIAWVGRKDPDFHVPTRRGRK"
     gene            complement(1016..2104)
                     /locus_tag="VGB57_02650"
     CDS             complement(1016..2104)
                     /locus_tag="VGB57_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY0130278.1"
                     /translation="MPTLFDPIQLGAVLCPNRCVMAPLTRGRATREHVPTELMVEYYR
                     QRSSAGLIVTEATGMSRQGLGWPYAPGIWSEEQREAWKPVVEAVHEAGGRIFCQLWHM
                     GRVVHPSFLGGESPVSASATTAPYKAHTYEGRQPYAEARPLRLDEIPGLLDDYSRAAA
                     YAIAAGFDGVQLHAANGYLIDQFLRDGTNFRDDDYGGPIENRIRLLTQATQAIADMVG
                     PERTAVRLSPNSETQGCQDSDPQALFTAAAAALAEIGIAFLELREPGPEGTFGKSDVP
                     PVAPAIRKVFQAPLILNSDFNLERAEAALEEERADAISFGRPFISNPDLPSRLLNGTE
                     LTPDVMATWYSQGPEGYTDYPEAEAVAA"
     gene            2187..3053
                     /locus_tag="VGB57_02655"
     CDS             2187..3053
                     /locus_tag="VGB57_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0130279.1"
                     /translation="MGRFAFPALIVGNLVLALGPWMVRLTDVGPVASGFWRLALAIPF
                     LFLLAWRQQAGREPPSRALVAMVAAGGLFLAIDLAAWHVGIGLTKLANASLFGNASSF
                     VLVVYGFVILKRLPRAVQAMALVLAVAGTGLLMGSSYELSPKNFRGDLFALAAGLLYT
                     GYLLAVDRARRTMAPWPVLAIATAAGALPLLLLSLALGQNVMPSDWTPLLILSFGSQV
                     IGQGLLVYAVGYLSPVVVGLGLLAQPAAGAVIGRFAYGETFSLADAAGAALIAVALVL
                     IRLPEKDSAKRI"
     gene            3266..3931
                     /locus_tag="VGB57_02660"
     CDS             3266..3931
                     /locus_tag="VGB57_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEY0130280.1"
                     /translation="MPEIAPLEMTLDELRMALAPLIPAHAVFDGWSDQALAMAAAELG
                     VPAPRARLAFPGGATEMIDAWFDAIDRGMATAFPLEKIESMKIRERIGGLVMYRLEVI
                     NPHKEALRRALAILAQPQNLPLAGRLGWRAADRMWRIAGDKATDFNHYSKRAILMGVY
                     GSTSLVYLDDGSEGLTATRAFLARRIDDVMRFEKVKAQWRGNRDRLPSLSRFLGRLRY
                     PAV"
     gene            3935..4339
                     /locus_tag="VGB57_02665"
     CDS             3935..4339
                     /locus_tag="VGB57_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEY0130281.1"
                     /translation="MEVRRIVEIASYHAHVYFDPATQRADALAIREAAAERFAVRLGT
                     TWDRPVGPHSKAMYQIAFAPELFASLVPWLMLNNRGLSILIHPNTTNQKRDHLLDAVW
                     IGSPLKLEGEVLPEEGEAELAGEVNTSPTLAP"
     gene            complement(4470..>4650)
                     /locus_tag="VGB57_02670"
     CDS             complement(4470..>4650)
                     /locus_tag="VGB57_02670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0130282.1"
                     /translation="TYPALLAELARRGWSDSDLAKLAGGNILRAMERVERVAAEMKNE
                     PPLNAAIEMLDGKRP"
BASE COUNT          688 a         1609 c         1600 g          753 t
ORIGIN      
        1 cgccttcccg caccactccg caggcgtagc ggtgcccata gtccgacatg gtcgagaggg
       61 ggccgatgag cggcggggcg acctggccgc aatgggtatg gccggcgagc atcaggaacg
      121 tgccgggcgc cgggtcggcg aacggatcgg ggctgtggct gagaatcagc ctgggaccct
      181 tgaggccctt caaggcggcg agcgtggccg ggaggtcggc gcggtcggtg aaatcgtcgt
      241 caaggcctcc gacagcgacg ggcccgacct gcaccgcgtc gttctccagc aggcggattc
      301 cggcgcgggc gagggcgcgc cgggccgctt cggcgtcgcg ccaatggtcg tgattgccga
      361 gcacggcgat cgtggccagc cgcgggcgga gcccggcgag cggcgccacc gcctcgtcgt
      421 gcgaataata acgtgttgcg agacgcttgt cggtgacgag gtcgccggcg atgaggacga
      481 tatcaggccg gagccggttg atctgctcga cgatccggcg cacccgcgcc ggcggcatgt
      541 ccggcccgcc gacatggacg tccgatatga gcaccagccg cagcggaagg gttccggacg
      601 gccagcccgc ggccgcgacc cggatggtgc ggactttcgg ctcccagacc gccgtcccgt
      661 agcaaaagag gagaagggcc gctcccgcaa gcgccgcgat cagaaggagg cgctggagga
      721 tccgccgcat cgtctcactt tcgcccccgc cgggtcggca catggaaatc cgggtccttg
      781 cgtcccaccc aggcgatgaa gcgcgcaagc tccggatgcg agcgcagagc cgccgccgag
      841 gcatagtccc gcgccagcgc cttgttgtcc agcgcccggt ggatggcgcg gtggcagacg
      901 ggatggaccg gcaccgtctc gcggccgccc cggctcttgg gcaccgaatg atgccattcc
      961 accgtcgtgc ccagcggacg gccgcacagg aagcaaggcg gcgcttcacc ctcgctcacg
     1021 cggcgacggc ctccgcctcc ggatagtcgg tatagccttc ggggccctgg ctgtaccagg
     1081 tcgccatcac gtccggcgtc agctccgtcc cgttgagcaa tcgcgacggc agatccgggt
     1141 tggagatgaa gggccgtccg aagctgatcg cgtccgcccg ctcctcttca agcgcggctt
     1201 ccgcccgctc gaggttgaag tcggaattga ggatcagggg cgcctggaag accttgcgga
     1261 tcgcgggggc cacgggcggt acgtcggact ttccgaacgt cccttccggc cccggttcgc
     1321 gaagctcgag gaaggcgatt ccgatctcgg ccaacgccgc cgcggcggcg gtgaacaagg
     1381 cttgcgggtc gctgtcctgg cagccctggg tctcgctgtt gggagagaga cgaaccgccg
     1441 tccgctccgg cccgaccatg tccgcgatgg cctgcgtcgc ttgtgtgaga agcctgatcc
     1501 ggttctcgat cgggccgccg taatcgtcgt cgcggaagtt ggtgccgtca cgcaggaact
     1561 ggtcgatcag atagccattg gcggcgtgga gctggacccc gtcgaacccc gccgcgatgg
     1621 cgtaagcggc ggctcgggaa taatcgtcga gcaggcccgg tatttcgtcg agccgcaacg
     1681 gccgggcctc ggcataaggc tggcgcccct cataggtgtg cgccttgtag ggtgcggtgg
     1741 tcgccgacgc cgacacgggc gactcgccgc cgaggaagct gggatggacg acccggccca
     1801 tatgccagag ctggcagaat atccgtccgc ccgcctcgtg aacggcctcg acgacgggct
     1861 tccatgcctc cctttgctcc tcgctccaga ttcccggcgc atagggccag ccgaggccct
     1921 gccggctcat tcccgtcgcc tcggtgacga tcagccctgc gctcgaacgc tggcgataat
     1981 attccaccat cagctcggtc ggcacatgct cgcgggtcgc ccggccgcgg gtgagcggcg
     2041 ccatgacaca tcgattgggg cagagcacgg cccccagctg gatcgggtcg aagagggtcg
     2101 gcatcggcat tccttgacgg ggaggagcgc ggacccctag cggcccggaa gcgacaatgc
     2161 agcaatctcg atcatccgcc ccggccgtgg gacgtttcgc gtttccggcg ctgatcgtgg
     2221 gcaacctcgt cctcgcgctg ggtccgtgga tggttcggct gacggacgtc ggcccggtcg
     2281 cctcgggctt ctggcggctg gccctcgcaa tccccttcct cttcctgctc gcgtggcggc
     2341 agcaggccgg gcgggagccg ccgtcccgcg ccctggtggc gatggtcgcc gcgggcggcc
     2401 tgttcctcgc catcgatctc gccgcctggc acgtcgggat cgggctgacc aagctcgcca
     2461 atgcgagcct gttcggaaac gcctccagct tcgtcctcgt cgtctacggc ttcgtcatcc
     2521 tgaagcgcct gccgcgcgcg gtgcaggcca tggccctcgt cctggccgtg gcgggcacgg
     2581 gcttgctgat gggatcgagc tacgaacttt cgccgaagaa cttcaggggc gacctcttcg
     2641 ccctcgccgc cgggctgctc tataccggct acctcctggc ggtggaccgg gcgcgaagga
     2701 cgatggcgcc ctggccggtg ctcgccatcg ccaccgccgc cggcgccctc cccctccttt
     2761 tgctctcttt ggcgctcggc cagaacgtaa tgccgagcga ctggaccccg cttctcatcc
     2821 tctccttcgg aagccaggtg atcggccagg ggcttctggt ctacgcggtc ggctatctgt
     2881 cgccggtggt ggtcggcctc ggcctcctcg cccagcccgc cgccggagcg gtgatcggcc
     2941 gcttcgccta tggcgagacg ttcagcctcg ccgacgccgc cggagcggcg ctgatcgcgg
     3001 tggcgctggt gctgatccgg cttccggaaa aggattccgc gaagcggatt tgagtatcgg
     3061 cgaccgctgt cgcaccgacc gctccccaac aattttgcgc gcagagacgc agagacgcgg
     3121 agggggccgt gcctccctcg agcgccgaag atcgccggcg gactctctgc gcctctgcgt
     3181 ctgcgcgatt caagattcaa acttggagcc ctgcggggac ctcgacgaac ttgcatcctt
     3241 ccccggcgag gatcattgac tctccatgcc cgaaatcgcc cccttggaga tgactctcga
     3301 cgagctgcgc atggcgctgg cgccgctcat tccggcccac gccgtgttcg acgggtggag
     3361 cgatcaggcg ctggccatgg cggcggccga gctcggagtg ccggcgccgc gggcccggct
     3421 cgctttcccg ggcggagcga ccgaaatgat cgacgcctgg ttcgatgcga tcgaccgggg
     3481 catggcgacc gccttcccgc tcgagaagat cgaatcgatg aagatccggg aacggatcgg
     3541 cgggctggtg atgtaccggc tcgaggtgat caacccgcac aaggaggcgc tccgccgcgc
     3601 cctcgccatt ctcgcccagc cgcagaacct gccgctcgcc ggccggctcg gctggcgcgc
     3661 ggccgaccgg atgtggcgga tcgccggcga caaggcgacc gacttcaacc attattccaa
     3721 gcgcgccata ttgatgggcg tctacggctc gaccagcctc gtctatctcg acgacggcag
     3781 cgaggggctg accgccaccc gcgccttcct cgcccgccgc atcgacgacg tcatgcgctt
     3841 cgagaaggtg aaggcgcagt ggcgcggcaa tcgcgaccgc ctcccctcgc tcagccgttt
     3901 cctcggccgg ctgcgctacc cggcggtctg agccgtggag gtccgccgca ttgtcgaaat
     3961 cgcgagctat cacgcccatg tctatttcga cccggcaacg cagcgcgcgg acgcgctggc
     4021 gattcgggag gcggcggcgg agcgtttcgc ggtcaggctt gggacgacgt gggaccggcc
     4081 ggtcggtccg cacagcaagg cgatgtacca gatcgccttc gcgcccgagc tgttcgcgag
     4141 ccttgtgccc tggctgatgc tgaacaaccg cgggctgagc atcctcatcc accccaatac
     4201 gaccaaccag aagcgcgacc atctgctcga cgcggtgtgg atcggctcgc cgctgaagct
     4261 ggagggggag gtgctgcccg aggagggcga ggcggagctg gcgggagagg tgaatacgtc
     4321 gccgacgctg gcgccgtaaa accctctccc tgcaggggag agggacaggc gagcgaagct
     4381 cgccagggtg agggcacacg cctccaaacc gccctcaccc tcccaccgct tcgcggcggg
     4441 gccctcctct cccctgcagg gagagggggt taaggccgct tcccgtccag catctcgatg
     4501 gcggcgttca gcggaggctc gttcttcatc tccgccgcga cccgctcgac gcgctccatc
     4561 gccctcagga tattgccgcc cgcgagcttc gccaggtcgg agtcgctcca gccgcgccgg
     4621 gcgagctcgg cgagcagggc cggataggtt
//
LOCUS       DASWNU010000138         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_498715, whole genome shotgun sequence.
ACCESSION   DASWNU010000138 DASWNU010000000
VERSION     DASWNU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498715"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VGB57_02675"
     CDS             <1..216
                     /locus_tag="VGB57_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0130283.1"
                     /translation="ACTLFGLTPEEALAGMTINAAKALGLEKEVGTISTGKAADLCVW
                     RVSRPAELAYWIGLPGPERRIVAGVDA"
     gene            308..>1743
                     /gene="hutU"
                     /locus_tag="VGB57_02680"
     CDS             308..>1743
                     /gene="hutU"
                     /locus_tag="VGB57_02680"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY0130284.1"
                     /translation="MNRRDNSRSIRARTGPGLVAKSWATEAAVRMLQNNLDPSVAEAP
                     EELVVYGGIGRAARDWASFDRIVETLERLEGDETLLVQSGKPVGVFRTHADAPRVLIA
                     NSNLVPKWATWEHFHELDRKGLMMYGQMTAGSWIYIGTQGIVQGTYETFVEMGRQHYG
                     GDLKGRWILTAGLGGMGGAQPLAAVMAGAHCLAIECQESRIEKRLETRYLDRRAGSLD
                     EALEILRTARSPVSVGLLGNAAELLPEMVRRGIRPDAVTDQTSAHDPVNGYLPAGWTV
                     EKWINMRERDPATVETAARESMAVHVNAMLAFRAMGIPTFDYGNNIRQVAKDQGVEHA
                     FDFPGFVPAYVRPLFCRGVGPFRWCALSGDPEDIYRTDAKVKALIPDDPHLHRWLDMA
                     RERIAFQGLPARICWVGLGQRHRLGMAFNAMVASGELKAPIVIGRDHLDSGSVASPNR
                     ETEAMQDGSDAVSDWPLLNALLNTASGAT"
BASE COUNT          293 a          570 c          611 g          269 t
ORIGIN      
        1 gcctgcaccc tgttcggcct gactcccgag gaggctttgg cgggaatgac catcaatgca
       61 gcgaaggcgc tggggctgga gaaggaagta ggcacgatct ccaccggcaa ggcggcggac
      121 ctgtgcgtct ggcgggtgag ccggccggcc gagctcgctt actggatcgg gctgccgggg
      181 ccggagcggc gcatcgtggc gggggtggat gcgtgaccgt cattgcgagc gaagcgaagc
      241 aatccagtac ggcctccggc gcagaactgg attgcttcgt cgcttcgctc ctcgcaatga
      301 cggcgccatg aatcgacgcg acaacagccg ctccatccgc gcccgcaccg ggcccggcct
      361 cgtcgccaag agctgggcca ccgaagcggc cgtccggatg ctccagaaca acctcgaccc
      421 ctcggtcgcc gaggcgcccg aggagctggt cgtctatggc gggatcggcc gggcggcccg
      481 ggactgggcg agcttcgacc ggatcgtcga gaccctggag cggctcgagg gggatgagac
      541 ccttctcgtt cagtcgggca agccggtcgg ggtcttccgc acccatgccg acgcgccgag
      601 ggtgctgatc gccaattcca acctggttcc gaaatgggcg acctgggagc atttccacga
      661 gctcgatcgc aagggcctga tgatgtacgg ccagatgacg gccggatcgt ggatctacat
      721 cggcacccag ggcatcgttc agggcaccta cgagaccttc gtcgagatgg ggcgccagca
      781 ttatggcggc gacttgaagg gccgctggat cctgaccgcg ggcctcggcg gaatgggcgg
      841 ggcccagccg ctggcggcgg tgatggcggg ggcgcattgc ctcgccatcg aatgccagga
      901 gagccggatc gagaagcggc tggagacgcg ctacctggat cgccgcgccg gatcgctcga
      961 cgaggcgctg gaaatcctcc ggacggcgcg aagcccggtt tccgtgggtc ttctcggaaa
     1021 cgccgccgaa ctcctgccgg agatggtccg gagaggcatt cgtccggacg cggtgaccga
     1081 ccagacctcg gcgcacgacc cggtcaacgg ctatctgccg gccggctgga ccgtcgaaaa
     1141 atggataaat atgcgcgagc gggacccggc cacggtggag acggctgcaa gagaatccat
     1201 ggcggtgcat gttaatgcaa tgctggcctt ccgggcgatg ggcatcccga ccttcgatta
     1261 cggcaacaac atcaggcagg tggcgaagga ccagggggtc gagcacgcct tcgatttccc
     1321 aggcttcgtt cccgcttacg tccggccgct cttctgccgc ggcgtaggtc cgttccggtg
     1381 gtgcgcactc tcgggggacc cggaggacat ctaccggacc gacgccaagg tgaaggcgct
     1441 gatccccgac gatccccacc tccaccgctg gctcgacatg gcgcgcgagc ggatcgcctt
     1501 ccaggggctt ccggcgcgga tctgctgggt cgggctcggg cagaggcacc ggctcgggat
     1561 ggcgttcaac gcgatggtcg cttcgggcga gctcaaggcg ccgatcgtca tcggccgcga
     1621 ccatctcgac agcggctcgg tcgcctcacc caaccgcgag acggaggcga tgcaggacgg
     1681 ctccgacgcc gtctccgact ggccgctcct caacgccctc ctcaacaccg cgtcgggcgc
     1741 cac
//
LOCUS       DASWNU010000139         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_187581, whole genome shotgun sequence.
ACCESSION   DASWNU010000139 DASWNU010000000
VERSION     DASWNU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187581"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1669
                     /locus_tag="VGB57_02685"
     CDS             95..1669
                     /locus_tag="VGB57_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY0130285.1"
                     /translation="MSQAALERRTFAIISHPDAGKTTLTEKLLYAAGAIHEAGEVRAR
                     GDRRRARSDWMAIEQQRGISVTSSVMTFERSGILFNLLDTPGHQDFSEDTYRTLTAVD
                     SAIMVIDAAKGIEAQTRKLFEVCRLRNIPIVTFVNKVDREGLSPFALLDEIAETLQLD
                     VCPRNWPAGMGGLFHGLYDLANPRLLLADRSDGIAPAAIVELTGLDDPALDSALPAHA
                     ADRLREEAGLAMAAYPEWDLDAFLAGDLTPVFFGSALREFGTGDLLDGLAAFAPPPGA
                     QPARSGPISPDAPGCSGFVFKVQANMDPNHRDRVAFLRICSGSLKRGMKLANPRLGKS
                     ISIQNPITFFARDRELAELARPGDIVGIPNHGTLRVGDTLSENGDIAFTGLPDFAPEI
                     LRRVRLDDPMKSKQMRRGLQDLAEEGVVRIFKPAIGTGWIVGVVGALQLDVLATRMEA
                     EYSLKLGFEPSPWEIARWVSADPAQMKRLTDTHRPSLAEDADGEAVLLIRNDWELSRF
                     QQDWPEVTFAAVRERH"
     gene            1915..>2376
                     /locus_tag="VGB57_02690"
     CDS             1915..>2376
                     /locus_tag="VGB57_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEY0130286.1"
                     /translation="MLRVYACITGEHDFRLVLVAGTICLLAAFTAFSIFEQARRAERR
                     AWCWTALAGFVAGTGIWATHFIAMLAYRPNLPIRYDLALTLLSIAAAVTITGAGWTAA
                     LRKGRSAALAAGTAIGIGIATMHYAGMAAVKLPGHFAWDRTMVAASLLIGIA"
BASE COUNT          374 a          881 c          734 g          387 t
ORIGIN      
        1 tacccaaagg cccctcgaac gcctatctgc accgctccac atcccgacgc cgccgccgcc
       61 ttttcgcgac cgcgcgacct ttccggagtt tgaaatgagc caagcggccc tcgagcggcg
      121 caccttcgcg atcatctcgc accccgacgc cggcaagacg accctgaccg agaagctcct
      181 ctatgccgcg ggcgcgatcc acgaggccgg ggaggtccgg gcgcgcggcg accgccgccg
      241 cgcccgatcg gactggatgg cgatcgagca gcagcgcggg atttcggtga cctcgtcggt
      301 gatgactttc gaacggtcgg gcatcctctt caacctgctc gacacgccgg ggcaccagga
      361 tttctcggaa gatacctacc ggacgctcac cgcggtcgac agcgcgatca tggtgatcga
      421 tgccgccaag gggatcgagg cgcaaacccg gaagctgttc gaggtctgcc gtcttcgcaa
      481 catcccgatc gtcacgttcg tcaacaaggt cgaccgcgag gggctgagcc cgttcgcgct
      541 tctcgacgag atcgccgaga cgcttcagct cgacgtctgc ccgcgcaact ggccggccgg
      601 aatgggcggc ctcttccacg gcctctacga cctcgccaac ccccgcctgc ttctcgccga
      661 ccggagcgac ggaatcgcgc ccgccgccat cgtcgagctc accgggctgg acgatccggc
      721 gctcgactcc gcgcttccgg cccacgccgc cgaccgcctt cgcgaggaag cgggcctggc
      781 gatggccgcc tatcccgaat gggatctcga cgccttcctg gcaggcgacc tcacgcccgt
      841 cttcttcggc tccgccctgc gcgagttcgg aaccggcgac ctgctcgacg gcctcgccgc
      901 cttcgccccg ccgcccggcg cccagccggc ccggtcgggg cccatctccc ccgacgctcc
      961 gggctgctcc ggcttcgtgt tcaaggtcca ggcgaacatg gaccccaatc atcgcgaccg
     1021 ggtcgccttc ctgcgtatct gctctggaag cctcaagcgc gggatgaagc tcgccaaccc
     1081 ccggctgggc aagtcgatct ctatccagaa tccgatcacc ttcttcgccc gcgaccgcga
     1141 gctggcggag ctcgcccgcc ccggcgacat cgtcggaatc cccaatcacg gcaccttgcg
     1201 ggtgggcgac acgctttcgg aaaatggcga catcgccttc accggcctgc ccgacttcgc
     1261 gccggagata ttgcgccggg tccgcctcga cgatccgatg aagtccaagc agatgcgccg
     1321 aggcctccag gacctcgccg aggaaggcgt cgtccggatc ttcaagcccg cgatcgggac
     1381 cggctggatc gtcggggtcg tcggagcgct ccagctcgac gttctggcga cccggatgga
     1441 ggcggaatat tcgctcaagc tcggcttcga gccgagcccc tgggagatcg cccgctgggt
     1501 ctccgccgat ccggcgcaga tgaagcgcct caccgacacc catcgcccca gccttgccga
     1561 ggatgccgac ggagaagcgg tcctgctcat ccgcaacgac tgggaattga gccgcttcca
     1621 gcaggactgg cccgaagtca ccttcgcagc ggtgcgcgag cggcattagc actcctgtcc
     1681 tccctgcggc gaagccgtgg ggagggggac cacacgaagt gtggtggagg ggtagaaggc
     1741 gcaggccgga gcggatcgct cgccaaacca ccaacgatac ggcgattacc cctacaccag
     1801 gcttcgcctg gtccccctcc ccacgcgctc cgcgcattgg aggattaggg ccatccttta
     1861 ccccttcttc accaaacccg ctccataagc accctttcac gggcgggcga aaacatgctt
     1921 cgggtctatg cctgcatcac gggcgaacat gatttccggc tcgtgctcgt cgccggcacc
     1981 atctgcctgc tcgccgcctt caccgccttc tccatcttcg agcaggcgcg gcgggcggag
     2041 cggcgggcct ggtgctggac ggccttggcc ggcttcgtcg ccgggaccgg catctgggcg
     2101 acccatttca tcgccatgct cgcctaccgg cccaacctgc cgatccgcta cgacctcgct
     2161 ttgaccctgc tttccatcgc cgcggcggtg acgatcaccg gcgcgggctg gaccgcggcc
     2221 ttgcgcaagg gccggagtgc cgccctcgcc gccggaacgg cgatcggaat cggaatcgcc
     2281 accatgcatt atgccgggat ggcggcggtg aagctgccgg gccacttcgc ctgggaccgg
     2341 acgatggtgg cggcctcgct gctcatcggg atcgcg
//
LOCUS       DASWNU010000140         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_109669, whole genome shotgun sequence.
ACCESSION   DASWNU010000140 DASWNU010000000
VERSION     DASWNU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109669"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..749
                     /locus_tag="VGB57_02695"
     CDS             348..749
                     /locus_tag="VGB57_02695"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0130287.1"
                     /translation="MTETEEVAAIPLVEERLEVGKRQVESGRVKVRVAVDTREEIVPA
                     ELARDEVEIRRVPRNVAVAELPGVRLEGDTTIIPVVEEQLVVEKRLVLVEEIHVVRRT
                     RTGVEEIPVTLRSERAEIEREERNVTEGAER"
     gene            746..1186
                     /locus_tag="VGB57_02700"
     CDS             746..1186
                     /locus_tag="VGB57_02700"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEY0130288.1"
                     /translation="MIAGDGSGREDGAPLQRAEWIDGGASADEVRVGDSRLVRGGARV
                     SVFTEGSPAIEAEAPGQGRILSEAEIAESGLLRERVIEIGEMREEAVVSKQAVVREEL
                     VVRRDVEQRTERFADTLRRTEVDVERLEPEEAVEGEGAGDAPGR"
     gene            1271..2152
                     /locus_tag="VGB57_02705"
     CDS             1271..2152
                     /locus_tag="VGB57_02705"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0130289.1"
                     /translation="MSRTVTALYDTRQEAEAARQRLASALDVERVNIIDQNSGGGGGG
                     SLGNLYLSNDDRHAYGEGLRRGGFLLCAEVESGEDHDRIIELLEQSAGVDLEERQQTW
                     QKEGWTPFSGQSASAGATTGGTRQAMSGATGSGNVVEEQRIPIVEEELRVGKREVTRG
                     GARVRSYVREIPVEEQVTLREEHVSVERRPVQQQQGVQSTQNSEDLLQDREIEMRETS
                     EEAVVQKVANVREELVVRKTAEEHVEQVSDTVRHTEVDVEEGAADRSAFGGFGSGEGR
                     TSGSEADRADFERDPSR"
     gene            2335..2820
                     /locus_tag="VGB57_02710"
     CDS             2335..2820
                     /locus_tag="VGB57_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY0130290.1"
                     /translation="MTEIFGSNPFDIVPHVAALVAAYLLALPVGWNREKEERSAGLRT
                     FPLVAVAACGFVQAAESLASEDPEAMARIVEGIITGMGFIGGGAILRLKDSVKGTATA
                     ASLWATGAVGTAAGLGSYDVAIVLSLITLLTLWVLSPLKTELVQEPDGVETREGDPGD
                     R"
     gene            complement(2838..>3731)
                     /locus_tag="VGB57_02715"
     CDS             complement(2838..>3731)
                     /locus_tag="VGB57_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0130291.1"
                     /translation="LERALGLHPRATYDFLDALVAIGMLHREGDGPAARYSSTASTAL
                     YLDRSSPHYVGGILEMANARLYRFWADLTPALKTGQPQNEVKHSQRPMFDELYADPVR
                     LEQFMDAMRGVSMANFEAFADRFDFTPYRTLADIGGATGQLSATVAGRHAHLACASYD
                     LPVVKPIAERRLAAAGLGDRVQAGEIDFFKEEFPKADLITMGMILHDWNLDHKKMLIA
                     KAYRALPEGGALVAIENLIDDPRRENAFGLLMSLNMLIEFGDAFDFTGADFTGWCLEA
                     GFRRCEILPLGGPASAAIAYK"
BASE COUNT          628 a         1168 c         1375 g          560 t
ORIGIN      
        1 cgtcctgctg caccggcgtg gcgtaagtgg tgtcggtggt gccggcggtc gtatcgttct
       61 gggcgagggc cgccgcgggg ctcagagtca gggccgccgc cagggccgcc tgcttcagta
      121 ccgaatgtcg catctgattt cctcctgttg gaactggccc ggttcgtccg ggcggtgcgg
      181 caaacggacg ggggaggggc ctgttccgaa catcgtcgaa gccggcgagt ggccccgatc
      241 cccgatcggc acgctgccgc aactctccgc cgcatataga aaatcggccg gaacgagcgg
      301 cgccgccgaa ggttgggcgg gtcgatgtaa gcatcgaggc gtaccccatg acagagactg
      361 aggaagtcgc ggcgatcccg ctcgtcgaag agcgtctgga ggtcggcaag aggcaggtcg
      421 agagcggccg ggtcaaggtg cgggtcgcgg tcgatacgcg cgaggagatc gtgcccgccg
      481 agctcgcccg tgacgaggtc gagatccgcc gggtgccgcg caacgtggcg gtcgccgagc
      541 ttcccggagt gcgtctcgag ggcgacacca cgatcatccc agtcgtcgaa gagcagctcg
      601 tcgtcgagaa gaggctggtt ctggtcgagg agatccacgt cgtccgccgc acccggaccg
      661 gggtggagga aatcccggtg actcttcgct ccgagcgggc cgagatcgag cgcgaggagc
      721 gaaacgtaac ggaaggcgcc gagcgatgat cgcgggcgac ggctcaggtc gcgaggacgg
      781 cgccccgctt cagcgcgccg agtggatcga cggcggcgcc tccgccgacg aggtccgggt
      841 cggcgattcc cggctggtca gaggcggcgc ccgcgtctcc gttttcacgg agggttcgcc
      901 tgccatcgaa gccgaggcgc ccggacaggg ccggatcctc agcgaagcag agatcgccga
      961 aagcggcctg ctccgggaaa gggtgatcga gatcggcgag atgcgcgagg aggcggtcgt
     1021 ctcgaagcag gccgtcgttc gcgaggagct ggttgtgcga agggatgtcg agcagcgcac
     1081 ggaacgcttc gccgacacct tgcgccggac cgaggtcgac gtcgaacggc tggagcccga
     1141 ggaagccgtt gaaggcgagg gcgccggaga cgctccgggc cgctgagccc ggccggagcg
     1201 aaagattgtc gcggcggcag gctcgccgcg aaccgaaacg agcggggaaa caacgaagga
     1261 gagtagcttt atgtcgcgca ccgtcaccgc cctctacgac acacgccagg aagccgaagc
     1321 cgcgcgccag cgtctggcct cggccctcga cgtcgagcga gtcaacatca tcgaccagaa
     1381 ttcgggaggc ggcggcggcg ggtcgctcgg caacctttat ctctcgaacg acgatcgcca
     1441 cgcttatggc gaaggccttc gccgcggcgg cttcctgctc tgcgccgagg tggagagcgg
     1501 cgaggatcac gaccgtatca tcgagctgct cgagcaatcc gccggagtcg atctcgaaga
     1561 gcggcagcag acctggcaga aggagggctg gacgcccttt tccggtcagt cggcaagcgc
     1621 gggcgcgacc accggcggca cccgccaggc gatgagcggc gctacgggga gcggcaatgt
     1681 cgtcgaggag cagcgcattc cgatcgtcga ggaggagctt cgtgtcggca agcgcgaagt
     1741 gacgcgcggc ggcgcgcggg tgcggtccta tgtgcgcgaa atcccggtcg aggagcaggt
     1801 gacccttcgc gaggagcatg tctcggtcga gcgtcgtccg gtccagcagc agcagggcgt
     1861 tcaatcgacc cagaatagcg aagacctgct ccaggatcgt gagatcgaga tgcgcgagac
     1921 ttcggaagag gcggtcgtcc agaaggtcgc caacgttcgt gaggagctgg tcgtccgcaa
     1981 gaccgccgaa gagcatgtcg agcaggtgag cgacaccgtc cgccacaccg aggtcgacgt
     2041 cgaggaaggc gctgccgacc gatccgcctt cggcggcttc ggcagcggcg aaggccggac
     2101 ttcgggctcc gaggccgaca gggccgattt cgagcgcgac ccctcccgtt gatcgagcaa
     2161 gggccggggc agagccgccc cggcccttgc ttcctgatcg tcatcccggg tcaagcccgg
     2221 gatgacgatc gttcgcgccc agccgtcacc atgaggccgc tccctcccgg ggcggccttt
     2281 gctttgagcg gccgtggcgg tagcgtagcc ggtcccggcg cacgagaggg tttcatgacg
     2341 gagattttcg gttcgaaccc tttcgacatc gtgccgcacg tggcggcgct ggtcgccgcc
     2401 tatctgctcg cattgccggt cggctggaac cgcgagaagg aggagcgcag cgccggcctc
     2461 cggacctttc cgctggtcgc ggtcgccgcc tgcggcttcg tccaggcggc ggaatcgctg
     2521 gcgagcgagg accccgaggc gatggcgagg atcgtcgagg gcatcatcac cgggatgggc
     2581 ttcatcggcg gcggcgccat ccttcgcctc aaggacagcg tcaagggcac ggccaccgcc
     2641 gccagcctgt gggcgacggg cgcggtgggc acggcggctg ggctgggcag ctacgacgtc
     2701 gcgatcgttc tttcgctgat cacgctgctg accttgtggg tcctctcgcc gctcaagacc
     2761 gagctggtcc aggagccgga cggggtggag actcgcgagg gggatccggg cgaccggtag
     2821 cggccgtccc gacccggcta tttgtacgcg atcgccgcgc tggccggccc gccgagcgga
     2881 aggatctcgc agcgcctgaa gccggcttcc aggcaccagc cggtgaaatc ggcgccggtg
     2941 aagtcgaacg cgtcgccgaa ctcgatcagc atgttcaggg acatgagcag gccgaaggcg
     3001 ttctcgcggc gggggtcgtc gatgaggttc tcgatggcga cgagggcgcc gccttcgggc
     3061 agcgcccggt aggccttggc gatcagcatc ttcttgtggt cgagattcca gtcgtggagg
     3121 atcattccca tcgtgatcag gtccgctttc ggaaactcct ccttgaagaa gtcgatctcc
     3181 ccggcttgca cccggtcgcc gagaccggcc gccgcgagcc gccgttcggc gatcggcttc
     3241 accaccggca aatcgtagga ggcgcaggcg agatgggcat ggcgcccggc gacggtcgcc
     3301 gaaagctggc cggtggcgcc gccgatgtcc gccagggtcc ggtagggggt gaagtcgaac
     3361 ctgtcggcga aggcctcgaa attggccatc gagaccccgc gcatcgcgtc catgaactgc
     3421 tcgagccgga cgggatcggc gtagagctcg tcgaacatcg gccgctgcga atgcttgacc
     3481 tcgttctgcg gctgccccgt cttcagcgcc ggagtgagat cggcccagaa gcggtagagg
     3541 cgggcattgg ccatttcgag gatcccgccg acatagtgcg ggctggagcg atcgagatag
     3601 agggcggtcg aggccgtgct cgaataacgc gcggcggggc cgtcgccctc gcggtggagc
     3661 atcccgatcg cgaccagggc atccaggaaa tcataggtgg cgcgggggtg gaggccgagc
     3721 gcccgctcaa g
//
LOCUS       DASWNU010000141         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_368243, whole genome shotgun sequence.
ACCESSION   DASWNU010000141 DASWNU010000000
VERSION     DASWNU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368243"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1728)
                     /locus_tag="VGB57_02720"
     CDS             complement(166..1728)
                     /locus_tag="VGB57_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130292.1"
                     /translation="MLRLRSILLLSTVISLAACGPGGVASPGEGTIVVPAPGTPAPPP
                     PPPPPGNGTPAAGCPQGTVDRGVIAAAFRNCEISGRISDDYTLQKLPGVIYSLAGPVN
                     VGTDVGGDGSLAGGDAVTLTIQAGVTVFGSSGNDYLVINRGSRINAVGSATQPIVFTA
                     RANVDGTATDSTQGLWGGVILLGRAPISDCNTAVPGGSAACQQVIEGTTGSFYGGNVA
                     TDNSGTMRFVQIRYSGFAIAPGNELQGLTMGGVGSNTTIDHIQIHNSSDDGIEIFGGR
                     VNAKHIVVTGADDDGLDTDLGYRGTIQFVIGVQRDGQNGDSMIEADSNGNEDALPRQY
                     TRVSNATFVQRSTVQGGNTILLRGGTDYALLNSVVVGTQACLDVDATGGTTLRATDAA
                     LQDVGPPLFRSVVLACPTPFRDDANVTVAEIQAIFGSGSNGNNPVFTPSLTNVFINGA
                     NETGVPATDPTPFNNDQFATANAAAPNRLETVAWIGAVRNASDTWYAGWTCNASYVSF
                     GSGGNCTAIPTS"
     gene            complement(1916..2593)
                     /locus_tag="VGB57_02725"
     CDS             complement(1916..2593)
                     /locus_tag="VGB57_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130293.1"
                     /translation="MARAVSFAAVAAASYAWLSASPAAQPPGPAPVTAPPPAPAPLPP
                     APPPAAAPIVSADGLILYGISGASGKAAAIIGSSQSGQRVVAVGSDYRPGLKLAEVGG
                     DYAVLQNGVQGIRLELSRYAAGGSSKATASLPVEEERKLEAAVLRNILKPVMANNRIG
                     GYALRPGESLPQLIRAGLQPGDVIMSVNGSQLDEERMSELAWQMGNARKTEFVFMRNG
                     KKMRASL"
     gene            complement(2612..3310)
                     /gene="phoB"
                     /locus_tag="VGB57_02730"
     CDS             complement(2612..3310)
                     /gene="phoB"
                     /locus_tag="VGB57_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY0130294.1"
                     /translation="MPSARTRLLLAEDNPAMVELLRFTFKKEGFDVVSTPSGEEALLL
                     ASESPPDIVILDWMLEELSGIEVCRRLRRAPESSNLPIIMLTARGEEADRVRGLETGA
                     DDYVTKPFSSPELIARVRAVLRRVRPALAGMVLSVGDLELDMAAHKVRRGGTAVAVGP
                     TEFKLLRHFLEHPGHVFSRERLLDAVWGRDSDIEARTVDVYVRRLRRAINMPGKDDLI
                     RTVRSAGYALDSEA"
     gene            complement(3412..4419)
                     /gene="virB11"
                     /locus_tag="VGB57_02735"
     CDS             complement(3412..4419)
                     /gene="virB11"
                     /locus_tag="VGB57_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HEY0130295.1"
                     /translation="MSLALAEDRLAGPGVYLRSFLAPLTGMLARPDVTDIYVNRPGEI
                     WVETTGGGIERHEAPGLDEATLARLARQIAALSHQGISREHPLLSATLPDGARIQIVA
                     PPATRGPLALAIRKHVSPELDLADYVQSGAFGETKARQGFERAQGDKALADMLAAGDV
                     GGMLAAAVRARKNILVAGGTSTGKTTFLNALLKEVPAEERLILIEDTPELFVRHENHV
                     GLISARSALGEAQVETDDLVAASLRMRPDRIILGELRGREAYSFLRAVNTGHPGSMTT
                     VHADSPERAVEQIVLLVLQGGTQLNRDDVRHYVSQTVDVFVQLTRSGGKRRVAEVVLR
                     D"
     gene            complement(4416..>4849)
                     /locus_tag="VGB57_02740"
     CDS             complement(4416..>4849)
                     /locus_tag="VGB57_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEY0130296.1"
                     /translation="DGTKVLIPRGSRLTGEYRSDIQPGQKRALINWTRLIRPDGVTMA
                     LGSPTADTLGRGGVRGKYNGHFFERFAGAILQSALDIGANVASRTAAGGVVVALPGST
                     QNLGQSLQSNRIIPTLTVPAGTSISVFVARDLDFRTAGGRR"
BASE COUNT          750 a         1561 c         1739 g          799 t
ORIGIN      
        1 ttccggcgct tcctgcgcga aggcggccgt cgccgtgagc gacgtggaca ggaggagcag
       61 gctccccagg cggatcggtt tcaacatcgt cggatatccc cggtgcataa gcggtaagga
      121 ggggcggcgg cgggcagggg ccgccgcccc gggcaggcgc tcggatcagg acgtgggaat
      181 ggccgtgcaa ttgccgccgc tgccgaagct cacgtagctc gcattgcagg tccagccggc
      241 ataccaggtg tcggaggcgt tgcggacggc gccgatccag gcgacggtct ccagccggtt
      301 cggcgccgcc gcattggccg tggcgaactg gtcgttgttg aacggagtcg ggtcggttgc
      361 cggcaccccg gtctcgttgg caccgttgat gaagacgttc gtcaggctcg gggtgaagac
      421 cgggttgttg ccgttcgagc ccgagccgaa gatcgcctgg atctccgcca cggtgacgtt
      481 ggcatcgtcc cggaacgggg tcggacaggc caggacgacc gagcggaaca gcggcgggcc
      541 gacgtcctgg agcgccgcgt ccgtggcccg gagcgtggtg ccgccggtcg cgtcgacgtc
      601 gaggcaggcc tgggtgccga cgacgacgct gttgagaagc gcataatcgg tgccgccgcg
      661 aagcaggatg gtgttgccgc cctggacggt gcttcgctgg acgaaggtgg cgttcgagac
      721 ccgcgtatac tggcgcggca gggcgtcttc gttgccgttc gaatcggctt cgatcatcga
      781 atcgccgttc tggccgtcgc gctggactcc gatcacgaac tggatcgtgc cgcgatagcc
      841 cagatcggtg tcgagcccgt cgtcgtcggc gccggtgacg acgatatgct tcgcgttcac
      901 ccggccgccg aaaatctcga tgccgtcgtc ggagctgttg tggatctgga tatggtcgat
      961 cgtcgtgttg cttccgacgc cgcccatggt gagcccctgg agctcgttgc ccggagcgat
     1021 ggcgaaaccg gaatagcgga tctggacgaa acgcatcgtt cccgaattgt cggtcgcgac
     1081 attgccgccg tagaaggagc cggtcgtgcc ctcgatcacc tgctggcagg cggccgaacc
     1141 gccggggacg gcggtgttgc agtcgctgat cggggcccgg ccgagaagga tgaccccgcc
     1201 ccacaggccc tgggtcgaat cggtcgcagt gccgtcgaca ttggcccggg cggtgaagac
     1261 gatcggctgg gtggcgcttc cgacggcgtt gatccgcgag cctcggttga tcaccagata
     1321 gtcgttgccg gacgatccga agacggtgac tcccgcctgg atggtcaggg tcaccgcgtc
     1381 gccgccggcg agactgccgt cgccgccgac atcggttccg acgttgaccg gcccggccag
     1441 cgaatagatc acgccgggca gcttctggag ggtgtagtcg tcgctgatcc gcccggagat
     1501 ctcgcaattg cggaaggcgg cggcgatgac tccccggtcc acggtgccct gcgggcagcc
     1561 tgcggccgga gtgccgttgc cgggaggcgg cgggggcggc ggcggggccg gagtgcccgg
     1621 ggctggaacg acgatagtcc cctcgccggg cgaggcgact ccgccggggc cgcatgcggc
     1681 cagggagatc acagtggaaa gcagaaggat cgagcggaga cgaagcatct aaaaccccct
     1741 ttaggatcga ctgaaggcag cccgcctctg gtgcgagtct gttcgagccg gacctaggga
     1801 gattctgtga tagtcatgtg acaaaatgcc acgacatgcg cctaccacag ggaaagatta
     1861 ttatgacaag cccggtctta tacgtgactt attattgtca ttatcgcgcg agacttcaaa
     1921 gagacgcgcg catcttcttg ccgttcctca tgaaaacgaa ctccgtcttc cgggcattgc
     1981 ccatttgcca ggcgagctcg ctcatccgct cctcgtcgag ctggctgccg ttgacgctca
     2041 tgatgacgtc gcccggctgc aggccggcgc ggatcagctg gggcaggctc tcgccgggcc
     2101 tgagcgcgta gccgccgatc cggttgttcg ccatgaccgg cttcaggatg ttgcgaagta
     2161 ccgcggcttc gagcttccgc tcctcttcga cgggcagtga ggccgtcgcc ttggacgacc
     2221 ctcccgccgc gtagcggcta agctcgagcc ggatcccctg aacgccgttt tgcaggacgg
     2281 cataatcccc tccgacctcg gcgagcttga gtccggggcg ataatcgctg ccgaccgcca
     2341 ccacgcgctg gccgctttgg ctcgacccga tgatcgccgc cgccttcccg ctcgccccgc
     2401 tgattccgta gaggatgagg ccatcggccg aaacgatcgg cgccgccgcg gggggaggag
     2461 cggggggaag cggggccggg gcaggcggcg gcgcggtcac cggagcgggt ccgggcggct
     2521 gtgcggccgg cgaggccgac agccaggcat aggaagcagc ggcgacggcc gcgaagctca
     2581 ccgctcgggc cacggctccg ccccgcaccg ctcaggcctc ggagtcgagc gcgtagccgg
     2641 ccgacctcac cgtccggatg agatcgtcct tgcccggcat gttgatcgcc cggcgaagcc
     2701 tgcggacata gacgtccacc gtccgggcct cgatgtcgct gtcccgcccc cacaccgcgt
     2761 cgagcagccg ctcgcgggag aagacgtggc cgggatgctc gaggaagtgg cgaagcagct
     2821 tgaattcggt cggcccgacg gcgacggcgg ttccgccgcg ccggaccttg tgcgccgcca
     2881 tgtccagctc gaggtctccg acgctgagca ccatccccgc cagcgccggc cggacccggc
     2941 ggagcacggc ccgcacccgg gcgatcagct cgggcgagct gaacggcttg gtgacgtaat
     3001 cgtcggcgcc ggtctcgagt ccgcgtaccc ggtcggcctc ctcgccgcgg gcggtgagca
     3061 tgatgatcgg cagattggac gattcgggcg ctcgccgaag ccgccggcag acctcgatcc
     3121 ccgacaattc ctccagcatc cagtcgagga tgacgatgtc gggcggcgat tccgacgcga
     3181 gcagaagggc ttcctcgccg ctcggagtgc tgaccacatc gaacccctcc ttcttgaagg
     3241 tgaagcgaag cagttcgacc attgcgggat tgtcttcggc gagaagcaat cgggtcctgg
     3301 ctgagggcat ggcttgtgct ttcggtcaaa tctcacgacc gaagtgcgcc actttcatga
     3361 caggcgtgtg aaagccgggg ggaacggcga aacagcggcc ggccgtccgc gctagtcgcg
     3421 gagcacgacc tcggcgaccc gccgcttccc gccgcttcgg gtcagctgga cgaagacgtc
     3481 gaccgtctgg ctgacataat ggcgaacgtc gtcgcggttg agctgggtcc cgccctggag
     3541 gacgagcagg acgatctgct cgaccgcgcg ctccgggctg tcggcatgga ccgtcgtcat
     3601 cgatccggga tggccggtgt tgacggcgcg caggaatgaa taggcttcgc ggcccctgag
     3661 ctcgcccagg atgatccggt cgggacgcat ccgaagcgag gcggcgacga gatcatccgt
     3721 ctcgacctgc gcctcgccga gcgcgcttcg ggcggagatc aggccgacat gattctcgtg
     3781 cctgacgaag agctcgggcg tgtcctcgat caggatcagc ctctcttcgg cgggcacctc
     3841 cttcaggagg gcgttgagga aggtcgtctt gccggtcgag gtgccgccgg cgacgaggat
     3901 gttcttgcgc gccctgacgg cggcggcgag cattccgccg acgtcgccgg cggcgagcat
     3961 gtcggccagc gccttgtccc cctgggcgcg ctcgaatccc tgcctcgctt tggtctcccc
     4021 gaaggcgccg gactggacgt agtcggccag gtcgagctcg ggcgagacgt gcttgcggat
     4081 cgcgagggcc agcgggccgc gggtggccgg cggagcgacg atctggatcc gggcgccgtc
     4141 gggaagagtg gcggaaagga ggggatgctc gcggctgatc ccctgatgcg acaaagcggc
     4201 gatctggcgc gcgagccgcg ccagggtcgc ctcgtcgagg ccgggcgcct cgtgccgctc
     4261 gatgcctccg cccgtggtct cgacccagat ttcgcccggc cggttcacgt agatgtcggt
     4321 gacgtccggc cgggccagca tcccggtcag cggagcgagg aaggagcgga ggtagactcc
     4381 cggtccggcg agcctgtcct ccgccaaagc gaggctcacc gccggcctcc ggcggttcgg
     4441 aagtcgaggt cgcgggcgac gaagacgctg atgctggttc cggccgggac ggtcagcgtc
     4501 gggatgatcc ggttggactg gaggctctgc cccagattct gggtcgagcc cggaagggcg
     4561 acgacgactc cgcccgccgc cgtccggctc gccacattgg ctccaatgtc gagggcggac
     4621 tggaggatcg cgcccgcgaa ccgctcgaag aaatgaccgt tatatttgcc ccgcacgccg
     4681 ccgcggccga gcgtgtcggc ggtgggcgaa ccgagcgcca tggtgactcc gtccggtcgg
     4741 atcaggcggg tccagttgat cagggccctt ttctggcccg gctggatgtc ggatcgatat
     4801 tcgccggtga ggcggctgcc gcgcgggatg agcaccttgg tgccgtcga
//
LOCUS       DASWNU010000142         7080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_368248, whole genome shotgun sequence.
ACCESSION   DASWNU010000142 DASWNU010000000
VERSION     DASWNU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368248"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1702)
                     /locus_tag="VGB57_02745"
     CDS             complement(<1..1702)
                     /locus_tag="VGB57_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY0130297.1"
                     /translation="MRPLESSIDTTPKAGWGAMRRFLPYLWPAGEPGLKARVVIAFLL
                     VLLAKGISLSTGFVYKGAIDIMSGDARGADAAVGLAVGLVLAYAGARFAGVLFENLRN
                     AIFERVGQDAARRLAERVFAHIHRLSLRFHLERRTGALTKIVERGTKSIDMMLYFLLF
                     NIAPTVVELAVICVIFGVKFGSGLVVATLVMVVAYIAFTRFVTEWRAQLQRRMNDVDN
                     QAIGRAVDSLLNYETVKYFGAEARESQRYGVAMRAFADASVKNETSLAWLNIGQSFIT
                     NLMMAGAMGYTVWGWSRGQFTTGDVVLVNTLLAQLFRPLDMLGWVYRTIRQGLIDMEA
                     MFALIDTPAEVKDAPGAPDLRLTQGRVRFEGVRFGYDPEREILHGIDLDVAPGTVTAV
                     VGPSGAGKSTLSRLLFRFYEPTGGRISIDDQDICQVTQSSLRSAIGIVPQDTVLFNDS
                     IGYNIGYGRDGATQAEIEGAARGASIHDFIMSLPDGYESQVGERGLKLSGGEKQRVAI
                     ARTLLKDPPILILDEATSALDSRTEAAIQETLRDVAAKRTSIVIAHRLSTIVDADQIV
                     V"
     gene            1951..2232
                     /locus_tag="VGB57_02750"
     CDS             1951..2232
                     /locus_tag="VGB57_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY0130298.1"
                     /translation="MTEPLLTVPAKEVASKFGYYTDEAMLHPVGIQRHGTTRVVMISL
                     KEYERLLRRDRQVLLVKDLDDETLEAIRTAQPSERSRELGRLLEDPGTG"
     gene            2144..2635
                     /locus_tag="VGB57_02755"
     CDS             2144..2635
                     /locus_tag="VGB57_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY0130299.1"
                     /translation="MRRSKPSGPRNPPSARASSGGSWKTPEPADVISYSYLWAREAAA
                     GEESGRKDRPAVVVVARIEAAAGPLILVAPVTHSAPEKATDAVEIPTAVKKQLGLDAD
                     RSWIVVTELNRFIWPGPDIRPMPETGAPFYDALPDWLFERVRSGVLQHSGRGRLKVTE
                     RTT"
     gene            2871..3146
                     /locus_tag="VGB57_02760"
     CDS             2871..3146
                     /locus_tag="VGB57_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130300.1"
                     /translation="MGPAIFIIAIMGCGEADAPCQQVRTLEPRYESQAACTAATDAAA
                     TQNSDVDYPVVVAQCVSAGAPAEAPRSGEIDRPGPGRADFKSSPVRS"
     gene            3311..5629
                     /locus_tag="VGB57_02765"
     CDS             3311..5629
                     /locus_tag="VGB57_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130301.1"
                     /translation="MTPVPASLRKLLPIFAAIAIALLVFAFAPQVGHAQDEEPIAYTG
                     HGGLFDRTGRQIVPTAEFVEEAQGWYRRRLLAQLPAEAKADFERLDRRLGDGLDLKGQ
                     NRLIARQRGLRALIAKVPRTVSNGRILAKLNALEAQLQWRLPRRENLEELRSLEPFQI
                     DPRLAERLKQPGFGPGISFLVTPNSGQAYMDECAAADVPVPPAIGDLDPNGTAGWKTL
                     GFIPRAQQFIVGSPAELRVFESPKGMCFALPRYSSSSMTTVSLDGVICLSKTTAKTCF
                     WDNSMNNGPSFSFPAGTQIPIGVADLNVDPQGRYQAGGAEIEFADGGQCTDCHAGENP
                     FIIHPDSDLGAGLFMSQLNEAPLNLPTFGPDRYVPLVGASWPQNTLSQTPAFVPGGCN
                     GCHQKGGAGRFPHLVPELQGYCNTVLAGAIQGRPQTPQGPGPVPATMPQGAPGTLAGT
                     VPVNDFRAWCGVPGTSGPSNRGDPHLTTTNGINYDFQAAGEFVSLRNSSNGFELQTRQ
                     SPVLTSFTPGANAYTGLASCVSLNTAAALRVGKHRITYQPARTRGARPEDMQLKIDGR
                     AVVLPRTGRNLGGANRIAATPSGGGLDVRLEDGTRVLITPNFWASQGYWYLNVEVLNT
                     PAREGTMGHILGADWLPRRPDGSSFGPAPASLPGRHGLLNGIFADAWRVSPTNSLFEY
                     AAGTSTASFTNRGWPPAPGGTCTAIRGNPIPGGQPRRPVKPINPEVAIRLCRPIQDKA
                     AFDNCVFDLTIMGDPNVAEGYRRTLGLRMGAP"
     gene            5737..5955
                     /locus_tag="VGB57_02770"
     CDS             5737..5955
                     /locus_tag="VGB57_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130302.1"
                     /translation="MAKNQHNPDSTDQALNQNNQMSDDPGDGSRKSFTVGRDGGRTAA
                     EKTPAQDQQDQSTIEAFGEEGAGIAAKE"
     gene            complement(5958..>7080)
                     /locus_tag="VGB57_02775"
     CDS             complement(5958..>7080)
                     /locus_tag="VGB57_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130303.1"
                     /translation="KLSDAQVTMRIAAGLATAGNAHTRLYILRNRTELRRLPIRLWWF
                     SDGLRVVRATAAHRDLLGCRVAHVAGLDPRVARDMVAPAFAGSPGWTDYMSVYALTSP
                     EALHGMGVTPALETVEYGFTDCKAAGDGRRLDPLALARSAKALEAWWDLSPKREPAAD
                     GWAHVLAGARALPLYLRNPNDHYWSEFEPASGLLYVQYNRSAEMEGKPIAAFADALAA
                     EFERRKVRALVVDLRFNTGGNLNLATAMMKRLEMLSRGLPRFVITGRATFSAGISHVA
                     FWREAGNVTLVGEPVGDEMDFWAEGGNIILPNSGLAAHFANGAHSYSAAPCPTPVPCL
                     DMNAPPIRPDLPVAASWADYRDGKDPALDSIVARLAAPR"
BASE COUNT         1236 a         2399 c         2336 g         1109 t
ORIGIN      
        1 ggacgacgat ctggtccgcg tcgacgatgg tcgagagccg gtgcgcgatg acgatcgagg
       61 tgcgtttcgc ggcgacgtcg cgcaaggtct cctggatcgc cgcctcggtc cggctgtcca
      121 gagcgctggt cgcctcgtcg aggatcagga tcggcgggtc cttgaggagc gtccgggcga
      181 tcgcgacgcg ctgcttctcg ccgccggaca gcttgagccc gcgctcgccg acctggcttt
      241 cgtagccgtc ggggaggctc atgatgaaat cgtggatcga cgccccccgg gcggcgccct
      301 cgatctccgc ctgcgtcgcg ccgtcgcggc cgtagccgat attgtagccg atggaatcgt
      361 tgaacagcac cgtgtcctgg gggacgatcc cgatcgccga gcgaaggctc gactgggtca
      421 cctggcagat gtcctggtcg tcgatggaaa tccggccgcc ggtcggctcg tagaaacgaa
      481 agagcaggcg cgacaaagtc gacttgccgg cgcccgacgg tccgaccacc gccgtcaccg
      541 tgcccggagc gacgtcgaga tcgatgccgt gcaatatctc tcgctccgga tcgtagccga
      601 agcggacccc ctcgaaccgg acccgcccct gcgtcagccg cagatccggc gcgcccgggg
      661 cgtccttcac ctcggccggg gtgtcgatga gggcgaacat cgcctccatg tcgatcaggc
      721 cctggcgaat ggtccggtag acccagccga gcatgtccag aggccggaag agctgtgcga
      781 gcagcgtgtt tacgaggacg acgtcgccgg tggtgaactg gccgcggctc cagccccaca
      841 ccgtgtagcc catcgcgccc gccatcatca gattggtgat gaaggactgg ccgatgttga
      901 gccaggcgag cgaggtctcg ttcttgaccg aagcgtcggc gaaggcgcgc atggcgactc
      961 cgtacctctg ggattcgcgg gcttcggcgc cgaaatattt gaccgtctcg taattgagca
     1021 gggaatcgac ggcgcggccg atcgcctggt tgtcgacgtc gttcatccgc cgctggagct
     1081 gcgcccgcca ttcggtgacg aagcgggtga aggcgatgta ggcgacgacc atcaccagcg
     1141 tcgcgacgac caggccggat ccgaacttta ctccgaatat gacgcagatg acggcgagct
     1201 cgaccacggt gggggcgatg ttgaacagca ggaaatagag catcatgtcg atgctcttgg
     1261 tgccgcgctc gacgatcttg gtgagcgctc ccgtgcgccg ctcgaggtgg aagcggagcg
     1321 acaggcggtg aatgtgggcg aacacccttt ccgccagccg ccgcgccgcg tcctgcccga
     1381 cccgctcgaa gatcgcgttg cgcagattct cgaacagcac cccggcaaag cgcgcgcccg
     1441 cgtaggccag caccagcccg acggcgagcc cgacggcggc gtcggcgccg cgcgcatcgc
     1501 ccgacatgat gtcgatcgca cccttgtaga cgaagccggt gctgagcgag atgcccttgg
     1561 cgaggagcac cagcaggaaa gcgatcacca cccgcgcctt cagccccggc tctcccgccg
     1621 gccacagata gggcaggaaa cgccgcatcg cgccccagcc ggccttcggg gtcgtgtcga
     1681 tcgaggattc gagggggcgc atcggatcta aggtaggagc gctagccgcc gaacccaagc
     1741 cgccctccac cctgcatgct gaacttgttt cagcatgcag gtggaggcgg cggtgaagcg
     1801 gcgaactgga ccctgaaata agttcagggt gacgacggag gcgccgccgg caagtcttga
     1861 ctgtacgtaa aagacgtata gtccgggcta cggctggaga tcaacggtta ggttgcctga
     1921 atccggcagc ggccagtcga ggaacgaagc atgacggaac cgctattgac ggtcccggcc
     1981 aaggaggtcg cgagcaagtt cggctattac acggacgaag ccatgcttca tcccgtgggt
     2041 atccagcgtc acggcaccac gcgtgtcgtg atgatctcgc tgaaggaata tgagcgcctc
     2101 ctgcgccgcg atcgccaggt tcttcttgtg aaggatctcg acgatgagac gctcgaagcc
     2161 atcaggaccg cgcaaccctc cgagcgctcg cgcgagctcg ggcggctcct ggaagacccc
     2221 ggaaccggct gacgtcatat cctactccta tctctgggcg cgggaagccg ctgccggaga
     2281 ggagagcggc cgcaaggacc ggccggcggt ggtggtggtt gcccggatcg aagcggcggc
     2341 gggacccttg atcctggtcg cgcccgtcac ccacagcgcg ccggaaaagg cgaccgatgc
     2401 ggtcgaaatt ccgaccgctg tcaagaaaca gctcggtctc gacgccgacc gcagctggat
     2461 cgtcgtgacg gagctaaatc gcttcatctg gccgggtccg gacatccgcc caatgcccga
     2521 aaccggcgcg cctttctacg atgccttgcc ggattggctg ttcgagcggg tgcggagcgg
     2581 tgttctccag cattccggcc gaggacggct caaggtcacc gaacggacga cgtaaactac
     2641 ctgcgtcagc tgaacttgtt cagcgaatcc cctttccggg cgggggacca acgagccctg
     2701 aaaccgtcga cgctgtaaca attgaaacga gaagcccctt ggcgcgacac agcagcatga
     2761 cgcgccaagc gcagacccga ccccacggcc attcggtcga agcacgcaaa accgccgcat
     2821 cgcctccgaa acgattggaa cggcggggat tcacatgggt ttagctgact atgggaccgg
     2881 caatcttcat tatcgcgatc atgggctgcg gcgaagccga cgcgccttgc cagcaggtgc
     2941 gcacgctcga gccgcgctac gagagccagg cggcctgcac cgcggcgacc gacgccgcgg
     3001 cgacccagaa cagcgatgtc gactatccgg tcgtcgtcgc gcaatgcgtc tcggcgggtg
     3061 cgccggccga ggcccctcgg agcggcgaaa tcgaccgccc cggacctgga cgggcggatt
     3121 tcaagtcaag cccggtgcga agctaagtct ttcttttaga cttgttttca attcgtaaga
     3181 cggcccaagc ttaatcttca gtcggctgaa agatcccgcc atttgacgtt tccgtaatgg
     3241 aagcgtaaca tccggctacc ttacatcgac tcgccttatt ggggggcgac ggatgtgaaa
     3301 ggagtttctc atgactcctg ttccagcttc acttcgcaag cttctgccga ttttcgccgc
     3361 gatcgccatc gcgctgcttg tcttcgcttt cgcgccgcag gtgggtcatg cccaggacga
     3421 ggagccgatc gcttataccg gtcacggcgg cctgttcgac cgaaccgggc gccagatcgt
     3481 gccgaccgct gaattcgtcg aggaagccca gggctggtat cgccggcgat tgctcgcgca
     3541 actgccagcc gaggccaagg cggatttcga gcgtctcgac cggcggctcg gcgacggcct
     3601 ggacctcaag ggccagaacc ggctgatcgc caggcagcgg gggctgcgcg ccttgatcgc
     3661 caaggtgccg cgaacggtgt cgaacggacg aatcctcgcg aagctgaacg cgctcgaggc
     3721 gcagctgcaa tggcggcttc cgcgacgcga gaatctcgag gagctgcgct cgctcgagcc
     3781 gttccagatc gatcccaggc ttgccgaaag gctgaagcag cccggcttcg gcccgggcat
     3841 ctccttcctc gtcacgccca atagcggcca ggcctatatg gacgagtgcg cggccgccga
     3901 cgtgcccgtc ccgccggcca tcggcgacct cgaccccaac ggcacggccg gctggaaaac
     3961 cctcggcttc attccccgcg cgcagcagtt catcgtcggc agccccgccg agctcagggt
     4021 gttcgagagc cccaagggca tgtgcttcgc cctgccccgc tactccagca gctcgatgac
     4081 gacggtctcg ctggacggcg tgatctgtct cagcaagacg acggccaaaa cttgcttctg
     4141 ggacaattcg atgaacaacg gccccagctt ctccttccct gccggaaccc agatcccgat
     4201 cggagtcgcc gatctcaacg tcgatccgca aggccgttat caggccggcg gggccgagat
     4261 cgaattcgcc gacggcggcc agtgcaccga ttgccacgcc ggcgagaacc ccttcatcat
     4321 ccacccggat tcggacctgg gcgccggcct gttcatgagc cagctcaacg aggcgccgct
     4381 caacctgccg accttcggac ccgaccgcta cgtccccctg gtcggcgcgt cctggccgca
     4441 gaacacattg tcccagacgc cggcattcgt tccggggggc tgcaacggct gtcatcaaaa
     4501 gggcggcgcc ggccgcttcc cgcacctcgt ccccgagctt cagggctatt gcaacacggt
     4561 gctcgcgggc gcgatccagg gccgtccgca gaccccgcag gggccgggcc cggtcccggc
     4621 gacgatgccc cagggcgcgc cgggcaccct ggcgggcacc gttccggtca acgacttcag
     4681 ggcctggtgc ggagttcccg gcacgtccgg cccgtcgaac cgcggcgatc cgcatctcac
     4741 caccacgaac ggcatcaatt acgacttcca ggcggccggc gagttcgtct cgctgcgcaa
     4801 ctcgtcgaac ggcttcgagc ttcagacccg gcaatccccg gtgctcacct ccttcactcc
     4861 gggagccaac gcctataccg ggctggcaag ctgcgtcagc ctcaacacgg cggccgccct
     4921 gcgcgtcggc aagcaccgca tcacctacca gccggccagg acccgcggtg cgaggccgga
     4981 ggacatgcag ctgaaaatcg atggacgggc ggtcgtattg ccccggaccg ggcgcaattt
     5041 gggcggcgcc aaccggatcg ccgcaacgcc gtccggcggc ggcctggatg tccgcctgga
     5101 ggacgggacg cgcgttctca tcacccccaa tttttgggcg tcccagggct attggtacct
     5161 caacgtcgaa gtactcaaca ctccggcgcg ggaaggcacg atggggcaca tcctcggcgc
     5221 ggactggctg ccccggcgcc cggacggctc ctccttcggc ccggctccgg cctcgcttcc
     5281 cggcaggcac ggcctactca acggcatctt cgccgacgcc tggcgggtga gcccgaccaa
     5341 cagcctgttc gagtatgcgg ccggcacctc gacggcgtcc ttcaccaatc gcggctggcc
     5401 gccggcgccg ggcggcacct gcacggcgat ccgcggcaat ccgattcccg gcggacagcc
     5461 gcgccggccg gtcaagccga tcaatccgga ggtggcgatc aggctctgcc ggccgatcca
     5521 ggacaaggcc gctttcgaca attgcgtgtt cgatctgacc atcatgggcg atccgaacgt
     5581 ggcggaaggg tatcggcgga cgctcgggct tcgcatggga gctccctgac ggacccgggc
     5641 cggctcaccc ggccggcgcc cgatccgaac cggcctcgcg ccccggaacc cgctggcgcg
     5701 cggtcggttt gctttccaaa cccttgagga agcacgatgg ccaagaacca gcacaatccc
     5761 gattcgaccg accaggcgct caaccagaac aaccagatgt ccgacgatcc cggcgacggc
     5821 agccgcaagt ccttcaccgt cggccgcgac ggcggccgga cggcggcgga gaagacaccg
     5881 gcgcaggacc agcaggacca gtcgaccatc gaggccttcg gcgaggaagg cgccgggatc
     5941 gcggcgaagg aataggctca gcgcggagcg gcgaggcggg cgacgatcga gtccagggcc
     6001 gggtccttgc cgtcgcggta atcggcccag ctcgcggcga ccgggagatc gggccggatc
     6061 ggtggcgcgt tcatgtcgag gcacggcacc ggcgtcgggc agggcgcggc cgagtagctg
     6121 tgggcgccgt tggcgaaatg cgccgccagg ccggaattgg gcaggatgat gttgcccccc
     6181 tccgcccaga aatccatctc gtcgccgacc ggctcgccga ccagggtgac gttcccggcc
     6241 tcgcgccaga aagcgacgtg gctgatcccc gcggaaaagg tggctcggcc ggtgatcacg
     6301 aatcgcggga gcccgcggct cagcatttcg agccgcttca tcatcgccgt ggcgagattg
     6361 aggttgccgc cggtgttgaa gcgcagatcg acgaccagcg ccctcacctt gcgccgttcg
     6421 aactcggccg cgagggcgtc ggcgaaggcg gcgatcggct tgccctccat ctcggccgac
     6481 cggttgtact ggacgtagag aaggccgctc gccggctcga attccgacca gtaatggtcg
     6541 ttcggattcc tcagatagag cggcagcgcc ctggcgccgg cgaggacgtg ggcccacccg
     6601 tccgccgccg gctcccgctt cggcgagagg tcccaccagg cttcgagcgc cttggcggac
     6661 cgggcgagcg cgagcggatc gaggcgccgg ccgtcgcccg cggccttgca gtcggtgaag
     6721 ccgtattcga ccgtctccag tgcgggcgtg acgcccatgc cgtgaagcgc ctccgggctc
     6781 gtcagcgcgt agacgctcat atagtcggtc cagccggggg agcccgcgaa ggcgggggcg
     6841 accatgtcgc gcgcgacgcg cggatcgagg cctgcgacat gcgccacccg gcatccgaga
     6901 aggtcgcgat gagcggcggt cgcgcgcacc accctcaggc cgtcgctgaa ccaccacaag
     6961 cggatcggca gccgccgcaa ctcggttcgg ttacgcagga tgtagaggcg ggtgtgggca
     7021 ttgcccgcgg tggcgagccc ggccgcgatc cgcatcgtca cctgagcgtc gctaagcttc
//
LOCUS       DASWNU010000143         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_290783, whole genome shotgun sequence.
ACCESSION   DASWNU010000143 DASWNU010000000
VERSION     DASWNU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290783"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="VGB57_02780"
     CDS             <1..1947
                     /locus_tag="VGB57_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY0130304.1"
                     /translation="RDFRWTDERFAELRRLGINPIVTLCHHGSGPAYTALCDPSFAPG
                     LAAHAAAVAARYPWIRDWTPVNEPLTTARFSALYGYWYPHTKDEGLFWTALLNEIDAT
                     RLAMREIRRINPEARLIQTDDLGYCHATEPLRAEADFQNERRWIGWDLLCGMVVPGHA
                     LFDRIAGFGLRGRLDAIAADPCPPDVIGINHYLASERWRDHRIELYPDRALADREVGH
                     GDGISFVDVDAIRFREEGVLGLPALIEQAWERYGRTVAITECHNGASREEQVRWFVEV
                     WEGAEALRAKGVDLCAVTAWSLLGSHDWNRVVTRFIGHYETGVFDCRTGTPRPTLLAP
                     VLMDLASGRKPSLPAIAGPGWWRRESRLVRATPKSEPEYELPIGARGGGAPAPLMIVG
                     DGGGLSRLAVRACEARGLYYVRCGDDAEARLAEASPWAVFDARDRHGLCGEGRDPACG
                     FALPRACAARALPCLTITGVAAWAPALDVPGLLEIRTRALFEPWDGSARAVALLEALD
                     SARRIEVDAASPWKGVYGPDLVDVALDLLLDGVSGPVSVLPGERLSEAEFAKALAVVA
                     DRDPDLVVATGSPPATPLFAWSDRTSYLPPWETTLERLVREARAARRLGVQGVDRRMD
                     EVREEEPAPPPGAISRPKSRVLGA"
     gene            complement(2051..2569)
                     /locus_tag="VGB57_02785"
     CDS             complement(2051..2569)
                     /locus_tag="VGB57_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0130305.1"
                     /translation="MSLKQFAETLPDYAKDLRLNISSLLNDQTLGDQRKYGLLLACAH
                     GTGYRPIVAAAEAEAEGKLSEQAANAARAAAAVMAMNNVYYRFVHLASNPEYGTLPAR
                     LRMNVIGAPGIEKEDFELFSLAVSAMNGCGMCIDSHERVLQQHKVKAETIQTAVRIAA
                     VVKAVATVHATL"
     gene            complement(2756..3295)
                     /locus_tag="VGB57_02790"
     CDS             complement(2756..3295)
                     /locus_tag="VGB57_02790"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0130306.1"
                     /translation="MLTVGDKIPSLTVPVQQGSALPAGETLDLGETNGKWKVLFYWPK
                     DFTFVCPTEIIGYGELAQDFADRDTVLIGASTDTDFVHHAWRKADERLAACDFPWIAD
                     NKKELANALGIIAQDEGVAFRATFIIDPDNVIQHVTVNGLNVGRNPQETLRILDALQT
                     DELCPCNWQEGEEVLRPAA"
BASE COUNT          470 a         1126 c         1171 g          568 t
ORIGIN      
        1 cgcgacttcc ggtggacaga cgaacggttc gccgagcttc gccgactcgg catcaacccg
       61 atcgtcacgc tctgccacca tggcagcggt ccggcctata ccgccctttg cgatccgagc
      121 ttcgcgcccg gcctcgcggc ccacgccgcg gccgtggccg cccgctatcc gtggatccgc
      181 gactggaccc cggtcaacga gccgctgacc accgcccgct tcagcgccct ttacggctat
      241 tggtacccgc atacgaagga cgaggggctg ttctggacgg cccttctcaa cgagatcgac
      301 gcgacccggc tggccatgcg cgagatccgc cggatcaacc cggaggcgag gctgatccag
      361 accgacgatc tcggctattg ccacgccacg gagccgctcc gggccgaggc cgatttccag
      421 aacgagcgcc gctggatcgg ctgggacctg ctctgcggaa tggtggtgcc gggccatgcc
      481 ctgttcgacc gcatcgccgg tttcggcctt cgcggccggc tcgacgcgat cgccgcggac
      541 ccgtgcccgc ccgacgtgat cggtatcaac cattatctcg ccagcgagcg ctggcgcgac
      601 catcgcatcg agctctaccc ggatcgggcc cttgccgacc gggaggtggg gcacggcgac
      661 gggatcagct tcgtcgacgt cgacgcgatc cgcttccgcg aggaaggcgt gctcggcctc
      721 cccgcgctca tcgagcaggc gtgggagcgt tacggccgca ccgtcgccat caccgaatgc
      781 cataacggcg cgtcccgcga ggagcaggtg cgctggttcg tggaggtgtg ggagggcgcc
      841 gaggcgctcc gggccaaggg cgtggatctg tgcgcggtga cggcctggtc gctgctcggc
      901 tcgcacgact ggaaccgggt ggtgacccgc ttcatcggcc attatgaaac gggcgtcttc
      961 gactgccgca ccggaacgcc gcgccccacc ctgctggcgc cggtgctgat ggacctcgcc
     1021 tccggccgca agccgtccct gcccgccatc gccggccccg gctggtggcg gcgggagtcg
     1081 agactcgtcc gggcgacgcc caaaagcgag ccggaatatg agctgccgat cggcgcccgc
     1141 ggcggcggcg ctccggcgcc tttgatgatc gtcggcgacg gcggcgggct cagccggctg
     1201 gcggtgcggg cgtgcgaggc gcgcggcctc tattacgtgc gctgcggcga cgacgccgaa
     1261 gcgcggctgg ccgaggcttc gccctgggcg gtgttcgacg cccgcgaccg tcacggcctg
     1321 tgcggcgagg gacgcgaccc ggcctgcggc ttcgcccttc cccgcgcctg cgccgctcga
     1381 gccctgccct gcctcacgat caccggagtc gccgcctggg ctccggccct ggacgtgccg
     1441 gggctcctgg aaatccgcac ccgcgccctg ttcgagccgt gggacggctc ggcgcgcgcg
     1501 gtcgctttgc tcgaggcgct ggattccgca aggcggatcg aagtcgacgc cgcttccccg
     1561 tggaagggtg tctacggccc cgatctcgtc gacgtcgctc tcgacctgct cctcgacggc
     1621 gtgagcggtc ccgtcagcgt gcttcccggg gagcgcctgt cagaggctga attcgcaaag
     1681 gccctcgccg tcgtcgccga tcgcgatccg gacctggtcg tcgccaccgg cagcccgccc
     1741 gccacgccgc tcttcgcctg gtcggaccgc acctcctacc ttccgccctg ggagacgacg
     1801 ctcgagcgcc tcgtgcgcga agcacgggcg gcgcggcggc tgggcgtgca gggcgtggat
     1861 cgccgcatgg acgaggtcag ggaggaggag cccgctccgc cacccggcgc catttcgcgc
     1921 cctaaatcga gggttctggg agcctgaatt agctcgtcat tgcgaggagc gaagcgacgc
     1981 ggcaatccag cgggcctccg atgccctgcc ggattgcttc gcatcgctcg caatgacggg
     2041 gagcgcgaat ctacaaagta gcgtggaccg tcgcgaccgc tttcacgacg gcggcgatgc
     2101 gaaccgcggt ctggatcgtc tccgccttga ccttgtgctg ctggaggacc cgctcgtggc
     2161 tgtcgatgca catgccgcag ccgttcatcg cgctgacggc gaggctgaac agctcgaaat
     2221 cctccttctc gatgccggga gcgccgatca cgttcatgcg caaccgggcc gggagggtgc
     2281 cgtattccgg gttcgaggcg agatggacga agcggtaata gacgttgttc atcgccatga
     2341 cggcggccgc ggctcgggcg gcattggccg cctgctcgct caatttgcct tccgcttccg
     2401 cctcggcggc ggcgacgatc ggccggtagc cggtgccgtg ggcgcaggcg agcaggaggc
     2461 cgtatttgcg ctggtcgccg agcgtctggt cgttgagcag ggaggagatg ttgagcctga
     2521 ggtccttcgc ataatcggga agggtttcgg cgaactgctt gagggacatg ggatgccttt
     2581 cgaaagactg tgttcgtcat cccggcgaaa gccgggacct cggggagacc caccgggact
     2641 tctcgtccag agttcccggc tttcgccggg atgacggata atgaaaaggg gcgcgggagc
     2701 cttggctccc gcgcccccca tcgcgaggtc tgctgaccgg cggccccagg ggggcttagg
     2761 ccgccgggcg caggacctcc tcgccttcct gccagttgca ggggcagagc tcgtcggtct
     2821 gaagcgcgtc gaggatgcgc agggtctcct gcgggttgcg gccgacgttg aggccgttca
     2881 ccgtgacgtg ctggatgacg ttgtcgggat cgatgatgaa ggtggcccgg aaggcgacgc
     2941 cctcgtcctg ggcgatgatt ccgagcgcgt tggccagctc cttcttgttg tccgcgatcc
     3001 acgggaagtc gcaggcggcg agccgctcat cggccttgcg ccaggcgtgg tggacgaagt
     3061 ccgtgtcggt cgaggcgccg atcagcacgg tgtcgcgatc cgcgaaatcc tgggcgagct
     3121 cgccatagcc gatgatctcg gtcgggcaga cgaaggtgaa atccttgggc cagtagaaga
     3181 ggaccttcca ctttccgttg gtttcgccga ggtcgagcgt ctcgcccgcg ggaagggcgc
     3241 tgccctgctg gacgggaacg gtgagagagg ggatcttatc gccgacggtg agcatgactg
     3301 cttcttttct cctgagtttc tagctggttg caccc
//
LOCUS       DASWNU010000144         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_134736, whole genome shotgun sequence.
ACCESSION   DASWNU010000144 DASWNU010000000
VERSION     DASWNU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134736"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VGB57_02795"
     CDS             complement(<1..1155)
                     /locus_tag="VGB57_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0130307.1"
                     /translation="MLSTAIMSLRLLPYCLLLLSLAACEQASAPVAPAAPAAVKAAAP
                     LQPVKVSADIAAFYRDRGARPLWVDRRGPRPEALRLAGEIARAADHGLDPARYDSAGL
                     AAALDAAKSGDPAARARADLLLSRAYVAFVRDLRVPARGKSSVTWVDEELAPEPPEVR
                     VLLQAAAAAPDLGRHVAEATAMNPLYTALSRGYARRRAAAPRLSAAEETAIRANLDRA
                     RLIPALPGRYVIVDAGSARLWMIDGGKVEGPMRVIVGKRSMQTPTMAGYIRYVTLNPW
                     WNVPPDLARERAKKVLKSGPGILRRENMELLSDWSDRPRVIPASQVDWKAVASGKTSL
                     RMRQRPGGGNVMGAMKFMFPNQLGIYLHDFPDKSLFGRADRFISSGCVRLE"
     gene            complement(1344..1631)
                     /locus_tag="VGB57_02800"
     CDS             complement(1344..1631)
                     /locus_tag="VGB57_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130308.1"
                     /translation="MRNVIAILAGLAVAGTLAFTGTPAAAADARCEAMPQQIRSALAN
                     AEPRVAQNATRRLRTGEALCRANNERGAAKEFQVALKLLGATGAGTSLAGK"
     gene            1712..2656
                     /locus_tag="VGB57_02805"
     CDS             1712..2656
                     /locus_tag="VGB57_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0130309.1"
                     /translation="MKQEYIIARNQLDGVEAFLRVAERRSFRAAADDLGLSPSAISQT
                     IRALEGRVGVPLLTRTTRSVGLTEAGERFIEHALPAFAGLTAAFEAARSFGERPAGLL
                     RLNVPRAAIPFLIEPVMEGFTAAFPDVEVEICGEDELIDLVERGFDAGIRMGDYLEAD
                     MIGIRLTPPFRLVVAGSPDYFERHGRPSEPADLRRHRCIRSRAPSTGAISRWHFDVAG
                     QPLEMAVEGPLIVNDLDVAKAAALRGVGLAYLAEPLVTEHLAAGRLELALEDHAAWSP
                     GAYLYYPSRTQMLPKLRAFIDFIREHRPPERFLEVKTA"
     gene            complement(2777..>3674)
                     /locus_tag="VGB57_02810"
     CDS             complement(2777..>3674)
                     /locus_tag="VGB57_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0130310.1"
                     /translation="GSWLDARFDAPRADRWLIHRLVLATAMLFVLVFGAAWWIARRLA
                     FPLRDLTRAAEGFRGHSPAEPVEPSGPSDLRRAVEAFNAMNRRTLALLDEKDRMLGAI
                     GHDLRTPLASLRIRAENMGPEEERERLVATVEEMAATLEDILTLARTGRAREPVRPVD
                     LAALADAVVEEFRDLGLAVEFRPSPRAVLAVQPNLLRRALRNLVDNALAYGGGGRVTV
                     EERSGRIEIRVEDDGPGIPADRLEEVLEPFRRLDSSRNRESGGAGLGLAIALAVAQAH
                     GGRLELANRVEGGLRASLILPK"
BASE COUNT          564 a         1261 c         1300 g          549 t
ORIGIN      
        1 ctcgagcctg acgcagcccg agctgatgaa ccggtcggcc cggccgaaca ggctcttgtc
       61 cgggaaatcg tggaggtaga tcccgagctg gttgggaaac atgaacttca tcgcgcccat
      121 gacattcccg ccgcccggcc gctggcgcat ccgaaggctg gtcttgccgc tcgccaccgc
      181 cttccagtcc acctgcgagg ccgggatcac gcgcggccga tcgctccagt cggacagcaa
      241 ttccatattc tcgcgcctga ggatcccggg gcccgacttg agcaccttct tcgcccgctc
      301 ccgagcgagg tccggcggga cgttccacca gggattgagg gttacgtagc gaatatagcc
      361 cgccatcgtc ggggtctgca tgcttcgctt gccgacgatg acccgcatcg ggccctcgac
      421 cttgccgccg tcgatcatcc acagacgcgc cgagccggcg tcgacgatga cgtagcggcc
      481 gggcagcgcc gggatcagcc gcgcgcggtc gagattggcc cggatcgccg tctcctcggc
      541 cgcggagaga cggggggcgg cggcgcggcg gcgggcatag ccgcggctca gcgccgtgta
      601 gagcgggttc atcgccgtcg cttcggcgac atggcggcca aggtccgggg cggcggcggc
      661 ggcctggagc aggacgcgga cttccggagg ctcgggagcg agctcctcgt cgacccaggt
      721 gaccgagctt ttgccgcggg cgggcactcg aaggtcgcgg acgaacgcca cataggcgcg
      781 cgagaggagc aggtcggcgc gggcgcgggc ggccggatcg cccgatttcg cggcgtcgag
      841 cgcggcggcg agcccggcgg agtcatagcg ggcgggatcg aggccgtggt cggcggcgcg
      901 ggcgatttcg ccggcgagcc ggagcgcctc cgggcggggc ccgcgccggt ccacccacag
      961 gggccgggcg ccgcggtcgc ggtagaaagc ggcgatgtcg gcggagactt tcaccggctg
     1021 gaggggggcg gcggctttta cagccgcagg tgcggccgga gcgacgggcg ccgaggcctg
     1081 ctcacaggcg gcgagcgaca gcaggaggag gcaataaggg agcaatcgca gcgacatgat
     1141 cgcggtcgat agcatgatag cacggacacg ctaaggcctc agcgtaaccc ctcccggatt
     1201 gggacgccct ctatcctccc tgcggcgaag ccgtggggag ggggaccacc gaagggtggt
     1261 ggaggggtaa ctgacgaggc ttctgccatc acccctccac cacacttcgt gcggtccccc
     1321 tccccatctt cgatgggaag gatttacttc ccggccaggc tcgtccccgc cccggtggcg
     1381 ccgagcagct tcagggccac ctggaattcc ttggcggcgc cgcgctcgtt gttggcgcgg
     1441 cacagggcct cgccggtgcg caggcggcgc gtcgcgttct gggcgaccct gggctcggcg
     1501 ttggcgaggg cgcttcggat ctgctgaggc atcgcttcgc agcgcgcgtc ggcggcggcg
     1561 gcgggagtgc cggtgaaagc gagcgtcccg gcgacagcga ggccggcgag gatggcgatg
     1621 acgttgcgca ttgatgaatt ccttccctga atgtggaacc gggataggcg cgctggattt
     1681 cttttataat ccgctataat gtcgatgggc catgaagcag gaatacataa tcgcccgaaa
     1741 ccagctcgac ggagtcgaag ctttcctgcg cgtggccgag cggcgcagct tccgggccgc
     1801 ggccgacgat ctggggcttt cgccctcggc gatcagccag acgatccggg cgctggaagg
     1861 acgggtcggc gttcccttgc tcacccggac cacccgaagc gtcggactga ccgaagcggg
     1921 cgagcgcttc atcgagcacg ccttgccggc cttcgccggc ctcaccgccg cgttcgaggc
     1981 ggcccgaagc ttcggcgagc ggccggccgg cctgcttcga ctgaacgtgc cccgcgccgc
     2041 aatccccttc ctgatcgagc cggtgatgga aggcttcacc gccgcttttc cggacgtcga
     2101 ggtcgagatt tgcggcgagg acgagctgat cgacctcgtc gagcgcggct tcgacgctgg
     2161 catcaggatg ggcgactatc tggaggcgga catgatcggg atccgcctca ccccgccctt
     2221 ccggctcgtc gtcgccggct cgcccgatta tttcgagagg cacggacggc cgtccgagcc
     2281 ggcggacctg cgccgccacc gctgcatccg ctcgcgcgcg ccgagcacgg gggcgatctc
     2341 ccgctggcat ttcgacgtcg ccggccagcc gctcgaaatg gcggtcgaag gtccgctgat
     2401 cgtcaacgac ctcgacgtcg ccaaggccgc cgcgcttcgc ggggtcggcc tcgcctatct
     2461 cgccgagccc ctggtgaccg agcatctcgc cgccggccgg ctggagctcg cgctcgagga
     2521 tcatgccgcc tggtcgccgg gcgcctatct ctattatcca agccgcaccc agatgcttcc
     2581 caagcttcga gccttcatcg acttcatccg cgagcaccgc ccgccggagc ggtttctgga
     2641 ggttaagacc gcctgatcgt catccctgga gggaccccat caaagtagta ctttgatggg
     2701 aacccgaaag ccgggacctc gggacaagaa ggcaccgcgg gtctccacca ggtcccggct
     2761 ttcgccggga tgacggttat ttcggcagga tcaggctggc gcgaaggccg ccctctaccc
     2821 ggttcgcaag ctccagcctt ccgccatgcg cctgagccac cgccaaagcg atggcgaggc
     2881 cgaggccggc gccgccgctt tcgcggttgc gcgaggaatc cagccggcga aagggctcga
     2941 gcacctcttc gagccgatcg gcggggatgc ccggaccgtc atcctctacc cgtatctcga
     3001 tccggcccga acgctcctcg accgtcaccc ggccgccgcc gccataagcg agcgcattgt
     3061 caaccaggtt gcgaagcgcc cgccggagca ggttcggctg caccgcgagg acggcgcgcg
     3121 gcgagggccg gaactcgacg gcgaggccga ggtcgcgaaa ctcctccacc accgcgtccg
     3181 ccaaagcggc gaggtcgacc gggcgcaccg gctcgcgcgc gcgcccggtg cgggcgaggg
     3241 tgaggatgtc ctcgagggtc gccgccatct cctcgacggt cgcgaccagc cgctcgcgct
     3301 cctcctcggg gcccatgttc tcggcccgga tccggagcga ggcgagcggc gttcgcaaat
     3361 cgtggccgat cgccccgagc atccggtcct tctcgtcgag cagggcgagg gttcgacggt
     3421 tcatcgcgtt gaacgcctcg accgctcgtc gaaggtcgga aggaccgctc ggctccaccg
     3481 gttcggcggg ggaatggccg cggaagccct cggcggcgcg ggtgaggtcg cgaagcggaa
     3541 acgccagccg ccgggcgatc caccaggccg ccccgaagac caggacgaac agcatcgccg
     3601 tggcaaggac gaggcggtgg atcagccagc gatcggcgcg cggcgcgtcg aaacgggcgt
     3661 cgagccagct tcca
//
LOCUS       DASWNU010000145         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_5964,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000145 DASWNU010000000
VERSION     DASWNU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5964"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VGB57_02815"
     CDS             complement(<1..1058)
                     /locus_tag="VGB57_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0130311.1"
                     /translation="MAGGNPNSGTGEVRPAHRFDAGRLEAWMRGHVEDYAGPLAVEQF
                     RGGQSNPTYRLTTPGRSYVLRRKPPGKLLPGAHAVDREYRVISALAGQGFPVARAFAL
                     CLDEEVIGTAFYVMEMVEGRIFWDPALPEVPRAERPAYFDAMNRTIAALHAIDPEAAG
                     LGDYGKPGNYFERQIARWSKQYQADVEAGRVAAMDRLVEWLPAHVPPDEPAPRVIHGD
                     FRCDNMIFHATEPRVLAVLDWELSTLGHPLADFSYHLMMYRMPEALGRHDLAALNIPS
                     EDDYVAAYCRRVGRDGIEHLDFYLAFNLFRLAAIVHGIKGRLARGNASSAHAGEMAKR
                     LEPLADLAWAQALKAEAAA"
     gene            1419..1931
                     /locus_tag="VGB57_02820"
     CDS             1419..1931
                     /locus_tag="VGB57_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY0130312.1"
                     /translation="MAEPFLAEIRMVSFNFAPKGWALCNGQLMPINQNQALFSLLGTN
                     FGGDGRVNFALPDYRGRTPIHVGSGFTLGERGGEQAHTLSISELPTHVHVLTASQTQG
                     DKSNPRPFNVPTLFATDPGNGYGPAQGLAALNPASVGNTGGSQAHLNMQPFLTINFCI
                     ALQGIFPSPN"
BASE COUNT          346 a          658 c          669 g          299 t
ORIGIN      
        1 gcggccgcct cggccttcag cgcctgcgcc caggcgagat cggcgagcgg ctccagccgc
       61 ttcgccatct cgccggcatg ggcggaggag gcgttgccgc gcgccagccg gcccttgatg
      121 ccgtggacga tggcggccag ccggaacagg ttgaacgcca ggtagaaatc gagatgctcg
      181 atgccgtcgc gcccgacgcg gcggcagtaa gcggcgacat aatcgtcctc ggaagggatg
      241 ttgagcgcgg cgaggtcgtg ccgccccagc gcctcgggca tccggtacat catcagatgg
      301 tagctgaaat cggcgagcgg gtggccgagc gtcgacagct cccagtccag caccgccagc
      361 accctcggct cggtcgcgtg gaagatcatg ttgtcgcagc gaaagtcgcc gtggatcacc
      421 cgcggcgccg gctcgtccgg cggcacgtgg gcgggcagcc attcgacgag ccgatccatc
      481 gccgctaccc ggccggcctc gacgtccgcc tgatactgct tcgaccagcg ggcgatctga
      541 cgctcgaaat aattgccggg cttgccatag tcgccgagcc ccgccgcctc cggatcgatc
      601 gcatggagcg cggcgatggt gcggttcatc gcgtcgaaat aggccggccg ctcggcgcgc
      661 gggacttcgg ggagggcagg gtcccagaag atccgcccct cgaccatctc catcacgtag
      721 aaagcggtgc cgatcacctc ctcgtcgagg cagagggcga aagcgcgggc gaccgggaag
      781 ccctggcccg ccagcgccga gatgacgcga tattcgcggt cgacggcatg ggcgccgggg
      841 aggagcttgc cgggcggctt gcgcctcagg acgtagctgc gcccgggcgt cgtcagccgg
      901 taggtggggt tggactggcc gcccctgaac tgctcgaccg ccagcgggcc cgcatagtcc
      961 tcgacatggc cgcgcatcca cgcctcgagc cggccggcgt cgaagcgatg ggcggggcgc
     1021 acctcgccgg tgccgctgtt gggattgccg ccggccatgc cctcagcccg cccggccggc
     1081 gggcgcggac ggggcaaagc ttacaaagct tgcacatgcc attcgcagcc gtgtccctcg
     1141 cgaaaagcac ggcgaggcgc ccgccgaggc acggcgggga gcggggccgg agaaggtcga
     1201 ttcgcgcgtc accggcggag ggtgacagac gattccctac attgccagcc cggccctact
     1261 cctgtggagt gcggcggcgg cagccgcggc gcggccggtc cgcgaccgaa acgagtccgg
     1321 gattgccgaa aacgacgaat ggaagaggcg acagcctgtt ttacgcgtat tgccacctcc
     1381 ccgattaact gacacatact tcgcaagggg ggtaccaaat ggcagaacct tttctcgcag
     1441 aaatccgaat ggtgtcgttc aacttcgccc cgaaagggtg ggcgctgtgc aacggccaat
     1501 tgatgccgat caaccagaac caggcattgt tctcgctatt gggaacgaac tttggcggcg
     1561 acgggcgcgt caacttcgcc cttccggatt acaggggccg caccccgatc cacgtcggaa
     1621 gcggcttcac gctcggcgag agaggcggcg agcaggcgca tacgctgagc atctccgagc
     1681 tgccgacgca tgtgcacgtg ctgacggcat cgcagacgca gggcgacaaa tcgaacccgc
     1741 gaccgttcaa cgttccgacg ctcttcgcta ccgatcccgg caacggctac ggtccggcgc
     1801 agggtcttgc ggcgcttaac ccggcgtcgg tcggcaacac cgggggcagc caggcgcacc
     1861 tcaacatgca gccgttcctg acgatcaatt tctgcatcgc cctccagggc atttttccgt
     1921 cgccgaactg agggggtcga aaccatggca cagccttata tcggtgaaat cc
//
LOCUS       DASWNU010000146         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_57244, whole genome shotgun sequence.
ACCESSION   DASWNU010000146 DASWNU010000000
VERSION     DASWNU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57244"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VGB57_02825"
     CDS             <1..760
                     /locus_tag="VGB57_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130313.1"
                     /translation="VAAGETPAARWTQPVCPEVLGLEDIGKRAAEAKIRRVAAAVGAA
                     VAPEICRRNIVVSFTSDGAALARGVVEREPWRVANLSPRTKSAILTGSAPIRWLYSTE
                     TRGRHGGSGTTSGNAAQSGPATHDGSGAGSSIGGDMPSLMHYESSILSTLTNRVLTSA
                     IVIIDTEEAMGRRLDSLAAYAALVALAEIRNADAAPAGSILTLFGSSGPPRDLTAQDL
                     GFLRALYRLPLDRQAMQHRGQLVHGITGALTARN"
     gene            complement(744..1283)
                     /locus_tag="VGB57_02830"
     CDS             complement(744..1283)
                     /locus_tag="VGB57_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HEY0130314.1"
                     /translation="MSTPPPWYEIALRELAACVAESGSGDHPRILEYLATCTDLDEGE
                     WERDSTPWCSAFVNWCLGQAGCEGTDSGWARSWAQWGEPVDPPRLGAIAVWARGRTSA
                     DAPVVTGHVSFFVEDLGDSLLVLGGNQSDCVCLKAYPKRGYLADTVGSAVPVHELYEL
                     IGYRWPVAAGGAPAPSSSR"
     gene            complement(1402..2100)
                     /gene="rplA"
                     /locus_tag="VGB57_02835"
     CDS             complement(1402..2100)
                     /gene="rplA"
                     /locus_tag="VGB57_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0130315.1"
                     /translation="MAKLSKNQKAIDAAVDRTRLYGVDEAIETLKANARSKFDETIEI
                     ALNLGVDPRHADQMVRGVVTLPAGTGKDVRVAVFARGAKAEEATAAGADVVGAEDLME
                     TIQGGTIDFDRVIATPDMMGIVGRLGKVLGPKGLMPNPKLGTVTPNVTEAVKAAKGGQ
                     VEFRVEKAGIIHSGIGKASFPAEDLKRNFTAFVDAIVRAKPAGAKGKYVKKVALSSTM
                     GPGLKVDVAEVAAA"
     gene            complement(2105..2536)
                     /gene="rplK"
                     /locus_tag="VGB57_02840"
     CDS             complement(2105..2536)
                     /gene="rplK"
                     /locus_tag="VGB57_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0130316.1"
                     /translation="MAKKITGYIKLQVPAGAANPSPPIGPALGQRGVNIMEFCKAFNA
                     ATGELEKGMPIPTVITVFADRSFAFETKTPPATFLLKKAAGLKSGSKEPGKIGAGRVT
                     RAQLREIAEMKMKDLNANDIEAASRIIEGSARAMGLEVTGS"
     gene            2978..3106
                     /locus_tag="VGB57_02845"
     CDS             2978..3106
                     /locus_tag="VGB57_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130317.1"
                     /translation="MDEIVRNLAPPLAVGLVTLVGYLWARGAHRRARQRRLAHPAE"
     gene            3103..3318
                     /locus_tag="VGB57_02850"
     CDS             3103..3318
                     /locus_tag="VGB57_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEY0130318.1"
                     /translation="MKLQHYPETDSLYIELKAGPGSETREVAPGVNADFAADGTIVGL
                     DIEGASRLDLGTLEAIDVPLTALKAAE"
     gene            complement(3355..3531)
                     /locus_tag="VGB57_02855"
     CDS             complement(3355..3531)
                     /locus_tag="VGB57_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130319.1"
                     /translation="MGCNARRTGTRTWKCRCCDARADYEYWACGAVTREWVVWREPCG
                     SCDDAPRTRPCPNR"
     gene            complement(3717..4421)
                     /locus_tag="VGB57_02860"
     CDS             complement(3717..4421)
                     /locus_tag="VGB57_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130320.1"
                     /translation="MAPDLESKQLLADFYLRMRDEQTRSAQHHETQRATVTTIYASLA
                     VADLALIGAIWQENGATLGLLGLALGLVVVGYVGFRIVVKLFERAMSHFSLAEAYLDS
                     LRVLLSDEAAAHLGNRLHEIKYVANATAELDAGELDEGMFDIGFPGGKRGEDIVTPEM
                     LRAELTRHNPIEPRPIVVPRHNRAVVLPDKPDVLQENYARVNLRKEWERVYLSFMFLG
                     AGLCIVIAIAWAVKVF"
BASE COUNT          753 a         1527 c         1507 g          781 t
ORIGIN      
        1 agtggcggcc ggggagactc ccgccgcccg ctggacccag cccgtctgcc ccgaagtgct
       61 cgggctcgag gatatcggca agcgcgccgc ggaagctaag atccgccggg tcgccgccgc
      121 cgtcggggcg gccgtggcgc ccgagatctg ccggcgcaac atcgtcgtca gcttcacctc
      181 cgacggcgca gctctcgccc gcggcgtggt ggagcgcgag ccctggcggg tcgccaatct
      241 ctcgcccagg accaagtcgg cgatactgac cggctcggcg ccgatacgct ggctctattc
      301 gaccgagacc cgcggccgcc acggcggctc gggcaccacc tcgggcaacg ccgcccaatc
      361 ggggccggcg acccatgacg gctccggcgc aggctcgtcg atcggcggcg acatgccctc
      421 cttgatgcat tacgaaagca gcatcctcag cacattgacc aaccgggtgc tgaccagcgc
      481 gatcgtcatc atcgacacgg aggaggcgat ggggcggcgg ctcgacagcc tggcggccta
      541 tgccgctctg gtcgccctcg ccgagatcag gaacgccgat gccgcgccgg cgggatcgat
      601 cctgaccctg ttcggttcct ccggccctcc gcgcgacctt accgcccagg acctcggctt
      661 ccttcgggcg ctgtaccggc tcccgctcga ccgccaggcg atgcagcatc gcggccagct
      721 ggtgcacggc atcaccggcg ctctcaccgc gaggaactag gcgcgggagc tccgccggca
      781 gcgaccggcc agcggtagcc gatcagctca taaagctcgt ggaccggcac cgccgagccg
      841 accgtgtcgg cgaggtagcc gcgtttcgga taggccttga ggcagacgca atcgctctgg
      901 ttgccaccca gcacgagcag gctgtcgccg agatcctcga cgaagaagga gacatggccg
      961 gtgaccaccg gcgcgtccgc cgaggtgcgc ccccgcgccc acaccgcgat cgccccgagc
     1021 cgcggcggat cgaccggctc gccccactgg gcccaggagc gggcccagcc cgaatcggtg
     1081 ccctcgcaac cagcctgccc caggcaccag ttgacgaaag ccgagcacca gggcgtagaa
     1141 tcccgctccc actcgccctc gtcgagatcc gtgcaggtgg cgagatattc gaggatccgc
     1201 ggatgatccc cgctcccgct ctcggcgacg caggcggcga gctcccgaag cgcgatctcg
     1261 taccaggggg gcggggtgga catggcggca agggtagcgg caccgccccg gcagaaaaag
     1321 gcctgacgaa aagggccgga gctttcgccc cggccctcgt gcaatctctc cggatcgaag
     1381 taccgcttcg atccgatccg cttacgccgc ggccacctcg gcgacgtcga ccttgaggcc
     1441 cgggcccatg gtcgagctca gggccacctt cttgacgtac ttgcccttgg cgccggcggg
     1501 cttcgccctg acgatggcgt cgacgaaggc ggtgaagttg cgcttcagat cctcggccgg
     1561 gaagctcgcc ttgccgatgc cggagtggat gatcccagcc ttctcgaccc ggaactcgac
     1621 ctggccgccc ttcgccgcct tgaccgcctc ggtgacattg ggggtgacgg tgccgagctt
     1681 cgggttcggc atcaggccct tggggcccag gaccttgccg agccggccga cgatgcccat
     1741 catgtcgggc gtggcgatga cccggtcgaa atcgatcgtc ccgccctgga tggtttccat
     1801 caggtcctcg gctccgacca cgtcggcgcc cgccgcagtg gcttcctcgg ccttggcgcc
     1861 gcgggcgaag accgccaccc ggacgtcctt gccggttccg gcgggtaagg tcaccactcc
     1921 gcggaccatc tggtcggcgt ggcgcggatc tacgccgagg ttgagggcga tctcgatcgt
     1981 ctcgtcgaac ttcgacctgg cgttggcctt caaggtctcg atcgcctcgt cgaccccgta
     2041 gagccgggtg cggtcgacgg ccgcatcgat cgctttctgg ttcttgctga gcttggccat
     2101 gtccttagct ccccgtcact tcgaggccca tggcccgggc cgagccctcg atgatccgcg
     2161 acgccgcctc gatgtcgttg gcgttgagat ccttcatctt catctcggcg atctcgcgaa
     2221 gctgggcgcg cgtgacccgg ccggcgccga tcttgcccgg ctccttggag cccgacttga
     2281 gccccgcggc cttcttcagc aaaaaggtcg ccggcggggt cttggtctcg aaggcgaagc
     2341 tccggtcggc gaagacggtg atgacggtgg ggatgggcat gcccttctcg agctcgcccg
     2401 tcgccgcgtt gaacgccttg cagaattcca tgatgttcac cccgcgctga ccgagcgcag
     2461 gcccgatcgg aggcgaggga ttggcggcgc ccgcgggcac ctggagcttg atatagcccg
     2521 tgatcttctt agccatgtgt ctcactctgt aggtcggccg aaagcggtac ttccggccgg
     2581 gtttcaagtt tagcggtcca gacggccctt ctcgcgaagc gcctcccgca ctttccattg
     2641 ctgctgcttt ggaagggggc gcccttagcg caaacggagg cggagcgcaa atcactgacg
     2701 ctgccgcgac acatcgaaaa gcgactggta actgccgtcg ggtttcggta gtcgaggtcg
     2761 cgacggagcg aacggccacc gagcgactgc gcgtcaacga gaagctgaag gcgctttcgt
     2821 cggtcatggc gaatctggga gcgggcctgt tcgccgcggc ggtcgcgcgc tggttcctga
     2881 cagggctgga cggctgggcg accgtctgga tcgttcggat tgacggctat atccgtggcg
     2941 atccagctaa tgtcgttcct ggagccggag agcgtcgatg gatgaaatcg ttcggaatct
     3001 ggcgccgccg ctcgcggtgg gcctggtcac tctcgtgggg tatctctggg cgcggggagc
     3061 gcaccggcgg gcccgtcagc ggcgcctggc gcacccggcg gagtgaagct gcaacattat
     3121 ccggagaccg acagcctcta tatcgagctg aaggcgggcc ccggcagcga aacccgcgag
     3181 gtggcgccgg gggtaaacgc ggatttcgcg gcggacggga cgatcgtggg cctcgacata
     3241 gaaggcgcga gcaggctcga cctcggcacc ctcgaggcca tcgacgtgcc gctgacggcg
     3301 ctcaaggcgg cggaataagg cgcatcggaa tggcgaattg ggagcaagtt gcggtcagcg
     3361 gttcgggcag ggcctggtcc tcggcgcgtc gtcgcagctg ccgcagggct cgcgccagac
     3421 cacccactcg cgggtgaccg ccccgcaggc ccaatattca tagtccgccc gggcgtcgca
     3481 gcagcgacac ttccaggtcc tggtgccggt gcgcctcgca ttgcagccca tcgcttccct
     3541 cgcgtcgctc tttacggctt ggcgacctta gcacgtttcc ccccgtgagg ggggaagaag
     3601 cctggattag gatcagaagg aggggacaga cacttaaggt gcacttttcg ggtttcacga
     3661 ccgggcgctc atcgaaaaaa catgatttga gtgtctgtcc caggcgccgg cttccactag
     3721 aaaaccttga ccgcccacgc gattgcaatg acgatgcaca gtcctgcgcc aaggaacatg
     3781 aacgacaggt agacccgctc ccactccttg cgcaggttta ccctcgcata attttcctgc
     3841 agcacgtccg gcttgtcggg gagcacgacg gcgcgattgt gccttgggac cacgatgggg
     3901 cgcggctcga tcgggttgtg ccgggtaagc tcagctctga gcatttcagg cgtcacaata
     3961 tcttcgccgc gcttgccgcc tggaaaaccg atgtcgaaca taccctcgtc gagctcccct
     4021 gcgtcaagct cggcggtagc gttcgccaca tatttgattt catggaggcg gttcccaagg
     4081 tgggcggccg cttcgtccga aagtaggact cgaagcgagt ctagataggc ctcggccagc
     4141 gagaagtgag acatggcccg ctcgaacagc ttcaccacga tccgaaaacc tacatagccg
     4201 acaacgacca gtccgagcgc taggccaagc aggccaagcg tggcgccgtt ctcctgccaa
     4261 atcgcaccga tcagcgcgag atcggctacg gccagcgacg catagatggt ggtgaccgtc
     4321 gcccgctgtg tctcgtggtg ttgcgccgac cgcgtctgct cgtcgcgcat ccgcaggtag
     4381 aaatccgcca gcagctgttt cgactcgagg tccggagcca tttgctcgat ccgtaagagt
     4441 ggagctgccc tcatgccctc caccaatcta gaaacgggtg gggcgtggtg gggttgcagg
     4501 ggtggcggcg ccgtgggacg aaatcactgt cccggataaa atggggacac gtgcgtttca
     4561 gcgcaaaa
//
LOCUS       DASWNU010000147         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_83347, whole genome shotgun sequence.
ACCESSION   DASWNU010000147 DASWNU010000000
VERSION     DASWNU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83347"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGB57_02865"
     CDS             <1..232
                     /locus_tag="VGB57_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130321.1"
                     /translation="RKMLRQSLKGLEGALEALAEVGIDSERRAETLTVEEFVAVAKAM
                     NSASPSPDGEGDHPKGGGGVTGAGLSVLRENP"
     gene            complement(306..809)
                     /locus_tag="VGB57_02870"
     CDS             complement(306..809)
                     /locus_tag="VGB57_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0130322.1"
                     /translation="MRLTPIALTVAIALATMASAGQGQKPDDQIDARSVALTQQGQAL
                     AAQARYQEATDLLETALALDPRNRQAYIGLAKAAQGLKLPGKAIKFYFEALKLEPNDV
                     AALTGQGEALVQRGAVERAKKNLERIRTLCANPCPQATQLAAVIAKGPPPEVMTAQRP
                     EVGAPKP"
     gene            complement(841..2028)
                     /locus_tag="VGB57_02875"
     CDS             complement(841..2028)
                     /locus_tag="VGB57_02875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY0130323.1"
                     /translation="MTPGARMQAAIELLDEIVAAAREGGAAADTLIARYFKTRRYAGS
                     GDRRAVRELVYRAIRAWPEPPSSGRAALLGLASADPALLAAFDGSPHGPAEASPGEKG
                     FTSPSSAVPKWMARRFDPAVTPADLRSLLDRAPLDLRVNRLRAEREELLPAFPGATAT
                     PLSPIGLRLPDAPQVEESEAWRTGLVEVQDEGSQLLALACAAAPGETVLDLCAGAGGK
                     TLALAAEMRNSGRLVASDTDRGRFSRMDLRLRRAGVTIVEPRLLDPGREREALADLVD
                     APDLILVDAPCSGTGTWRRNPELRWRLDPDRLVRYVKLQAYLLDLAAELIRPGGRIVY
                     AVCSLLADEGRGQSAAFEGRSAMVPESLPIAAGRAAGSGRILTPAQDGTDGFFVARWR
                     APC"
     gene            complement(2032..3492)
                     /gene="guaB"
                     /locus_tag="VGB57_02880"
     CDS             complement(2032..3492)
                     /gene="guaB"
                     /locus_tag="VGB57_02880"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0130324.1"
                     /translation="MDIRLGLTFDDVLLQPGESSVLPSQADTSTQVTREIRLNIPILS
                     SAMDTVTEADMAIVMAQLGGMGVLHRNLTVEEQAAAVRAVKRFESGMVVNPITMTPEQ
                     TLADALELMQRHRISGIPVTEKGGRLVGILTNRDVRFAENPRQPVAELMTRENLATVG
                     PGVTQEEARRLLHQRRIEKLIVVDEAGRCTGLITVKDIEKAVTYPNATKDAAGRLRVA
                     AATTVGDKGFERSEALIDAECDLIVIDTAHGHNREVAAAVERIKKLSNAVQIVAGNVA
                     TGEATRALVGAGADAVKVGIGPGSICTTRVVAGVGVPQLTAIMDAAEAAAASGTPVIA
                     DGGLRTSGDLAKALAAGASTVMAGSLLAGTEEAPGETFLYQGRSYKSYRGMGSVGAMA
                     RGSADRYFQHDIKDQLKLVPEGIEGQVPYKGHAKDVIHQLVGGIKAAMGYTGAATLEE
                     LRSRAKFVRITNAGLRESHVHDVAITREAPNYPTSG"
     gene            3750..4022
                     /locus_tag="VGB57_02885"
     CDS             3750..4022
                     /locus_tag="VGB57_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130325.1"
                     /translation="MIVPRGFDQAETLRAARERCGRAALCQVFAYTDEVYLKPDVPED
                     ERWRATVLAYSRNWNSGFEQILWDCSIYRDTDRSQCRGEPIVDPEL"
BASE COUNT          643 a         1374 c         1576 g          690 t
ORIGIN      
        1 gcgcaagatg ctgaggcaga gcctgaaggg actggagggg gcgctggagg cgctggcgga
       61 ggtggggata gactccgaac ggcgggcgga gactctgacg gtggaggagt tcgtcgcagt
      121 ggcgaaggcg atgaattctg cctccccatc gccagatggg gagggggacc acccgaaggg
      181 tggtggaggg gtaactggcg caggcttgag cgtattgcgc gagaacccct gagtcctcgg
      241 cagctacccc tccaccatgc ttcgcatggt ccccctcccc acggcttcgc cgcagggagg
      301 cagaatcaag gcttcggcgc gcccacttcg ggccgctggg cggtcatgac ctcgggcggc
      361 ggacccttgg cgatgacggc ggcgagctgg gtcgcctgcg ggcaggggtt ggcgcagagc
      421 gtcctgatcc gctcgagatt cttcttggcc cgctcgaccg ctccgcgctg gaccagcgcc
      481 tcgccctggc cggtgagggc ggcgacgtcg ttgggctcga gcttcagggc ctcgaaatag
      541 aatttgatcg ccttgccggg gagcttgagc ccctgcgcgg ccttggcgag gccgatatag
      601 gcctggcggt tgcgcggatc gagggcgaga gccgtttcca ggaggtcggt ggcttcctgg
      661 tagcgcgcct gagcggccag cgcctgcccc tgctgggtga gcgcgaccga acgggcgtcg
      721 atctggtcgt cgggcttctg gccctggccg gcgctcgcca tggtcgccag cgcgatggcg
      781 acggtgagtg cgatgggagt gaggcgcatc agtaactcca tgatcctgtc gcgaagcggt
      841 ttagcatggc gcccgccacc gcgcgacgaa aaagccgtcc gttccgtcct gcgccggggt
      901 gaggatccgc cccgagccgg cggcccgtcc agccgcgatg gggaggcttt ccggaaccat
      961 ggctgaacgg ccttcgaatg ccgccgactg gcccctgccc tcatccgcca gaagcgagca
     1021 gaccgcgtag acgatacgtc cgccgggcct gatcagttcc gccgccaggt caagcagata
     1081 ggcctgaagc ttcacatatc ggacgaggcg atcgggatcg agccgccagc gcagctcggg
     1141 gttgcgccgc caggtgccgg tccccgagca gggcgcgtcg acgaggatca gatcgggggc
     1201 gtcgacgagg tcggccagag cctcgcgctc gcggcccgga tcgagcaatc gaggctccac
     1261 gatcgtcacc cccgcgcgcc tgagacgaag gtccatccgc gagaaccggc cgcggtcggt
     1321 gtccgaagcg acgaggcggc ctgaattgcg catttcggcg gcgagagcga gcgtcttgcc
     1381 gccggcgccg gcgcaaaggt cgaggacggt ctcgccgggg gccgcggcgc aggccaaagc
     1441 gaggagctgg ctgccttcgt cctgaacctc gacgaggccg gtgcgccagg cttcgctctc
     1501 ctccacctgg ggggcgtcgg gaagccggag gccgatcggc gacaagggcg tcgcagtcgc
     1561 gcccggaaag gcgggcagca attcctcgcg ctcggcgcgg aggcggttga cgcgcaggtc
     1621 caggggcgcg cggtcgagca gcgagcgcag gtcggcgggg gtcacggccg ggtcgaagcg
     1681 gcgggccatc catttcggta ccgccgacga cggggacgtg aagccctttt ccccgggcga
     1741 ggcttcggcg gggccgtgcg gcgagccgtc gaacgcggcg agcagcgccg gatccgccga
     1801 cgccaggccg agcagggcgg cccggcccga ggacggcggc tcgggccagg cccggatcgc
     1861 tcggtagacg agctcgcgca cagcgcggcg gtcgcccgaa ccggcatagc ggcgagtctt
     1921 gaaatagcgg gcgatgagcg tgtcggcggc agccccccct tccctcgccg ccgcgacgat
     1981 ttcatcgagc agctcgatcg ccgcctgcat ccgtgcgccg ggggtcatgg ctcagcccga
     2041 ggtcggataa ttgggcgctt cccgggtgat ggcgacgtcg tggacatggc tttcgcgaag
     2101 gccggcattg gtgatccgga cgaactttgc cctggagcga agctcctcga gggtggcggc
     2161 gccggtatag cccatcgccg ccttgattcc gccgacgagc tggtggatca cgtccttcgc
     2221 atggcccttg taggggacct ggccctcgat cccttcgggg acgagcttca gctggtcctt
     2281 gatgtcgtgc tggaaatagc ggtcggccga cccgcgcgcc atcgcgccca ccgaacccat
     2341 accgcgatag gatttgtagg agcggccctg gtagaggaag gtctcgcccg gcgcctcctc
     2401 ggtgccggcg aggagcgagc ccgccatcac cgtcgaagcg ccggcggcga gcgccttggc
     2461 gaggtcgccg ctggtgcgaa ggccgccgtc ggcgatgacc ggggtgccgg aagcggcagc
     2521 ggcctcggcg gcgtccatga tcgcggtcag ctgcggaact ccgactccgg ccacaacacg
     2581 ggtggtgcag atcgagcccg ggccgatgcc gaccttgacc gcgtccgccc ccgccccgac
     2641 cagggccctg gtcgcctcgc cggtggcgac gttgccggcg acgatctgga ccgcgttgga
     2701 caatttcttg atccgttcga ccgcggcggc gacctcgcgg ttatggccgt gggcggtatc
     2761 gatgacgatg aggtcgcatt ccgcgtcgat cagggcctcg gaacgctcga accccttgtc
     2821 gccgaccgtc gtcgccgcgg cgacgcggag gcggccggcg gcgtctttgg tcgcgttggg
     2881 ataggtgacc gccttctcga tgtccttgac ggtgatgagg ccggtgcagc ggccggcctc
     2941 gtcgacgacg atcaatttct cgatccggcg ctggtggagc aggcgccggg cctcctcctg
     3001 ggtgactccg ggaccgaccg tggcgagatt ctcgcgggtc atcagctcgg cgaccggctg
     3061 gcgcggattc tcggcgaagc ggacgtcgcg gttggtgagg atccccacga gccggccgcc
     3121 tttctcggtg accggaatgc cggagatgcg gtgccgctgc atcagctcga gcgcgtcggc
     3181 gagcgtctgt tccggggtca tggtgatcgg gttgaccacc atcccgctct cgaagcgctt
     3241 caccgccctg accgccgccg cctgctcctc gacggtgagg ttgcgatgga gcacccccat
     3301 cccgcccagt tgcgccatga cgatcgccat gtcggcctcg gtcaccgtgt ccatcgccga
     3361 ggagaggatg gggatgttga ggcggatttc gcgggtcacc tgggtcgagg tgtcggcctg
     3421 gctcggaagc accgacgatt cgccgggctg aagcagcacg tcgtcgaagg tgaggcccag
     3481 cctgatgtcc attccatgcc tcgcgaggat tcggtggggt tctataggag cgggttgggc
     3541 ggggcgccag agcgctgggg cggcaatgga aatcctgtta gcatggccgg accggtccgg
     3601 cgcttcgagg cggcggcgcg tggttgggga ggcgagagtt ggctaggtcg tggaggttga
     3661 gcctgctggt cgccgcgagc gctgtcgccg ccagcggctg cgaggagccg tcgccgactt
     3721 ttcacgcccg acaggcgggt tcatgggaca tgatcgtccc gcggggcttc gaccaggcgg
     3781 aaaccctccg cgccgctagg gagcgttgcg gcagggcagc cctttgtcag gtcttcgctt
     3841 atacggacga ggtctatctc aagccggacg tccccgagga cgaacgctgg cgcgccaccg
     3901 tccttgccta ttcgcgaaat tggaacagcg gcttcgagca gatcctgtgg gattgttcga
     3961 tctatcggga taccgaccga agccagtgcc gcggcgagcc gatcgtcgat cctgaactct
     4021 gagatggcgc aagccctgtc tcgcacctgc gggccttcac accgcctgat cgcggcgaag
     4081 gcgctagaac atcgtgcgtt aaacttgaat cgtcaccccg ggcttggccc ggggtccatc
     4141 ttcttgcccg gcggtaccga aaaagaagat ggatgccggg tcaagcccgg catgacgagg
     4201 tgaggcagat ccagcgcacg ccgctctggg tagccgaagg caaaaagaag cggccgatcc
     4261 gctgaggacc ggccgcttcg ttt
//
LOCUS       DASWNU010000148         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_160811, whole genome shotgun sequence.
ACCESSION   DASWNU010000148 DASWNU010000000
VERSION     DASWNU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160811"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VGB57_02890"
     CDS             <1..516
                     /locus_tag="VGB57_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0130326.1"
                     /translation="GRRILPTRQEEFYFAAPAGDRRFLPGAMPGWADFNGGDIFYGFP
                     DLESRGLKFAHDQHGVEIDPDTQDRRPTQAALAEIIAFRDRRFPLLRGAPLTESRVCQ
                     YENSSNGDFLIDFHPRLPNVLLVGGGSGHGFKHGPEVGRYAAARLLGGTAVEPRFSLA
                     TKAETHKREVH"
     gene            complement(513..1067)
                     /gene="grpE"
                     /locus_tag="VGB57_02895"
     CDS             complement(513..1067)
                     /gene="grpE"
                     /locus_tag="VGB57_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0130327.1"
                     /translation="MNDHEDLHEEAEEIRKETAEAAPELAEHDRVVELEKALEDATSK
                     ALYAAAETQNVRRRLEAEKVQAAAYASSAFARDMLAIKDHLDRALAAVSDELRADETA
                     ANFLAGIEATARELEAAFTRQGITRIQSVGEKLDPHRHQAMMEIPTENAEPGTIVDEM
                     QAGYMLKDRLLRPALVGVAKAPDA"
     gene            complement(1076..>1689)
                     /gene="hrcA"
                     /locus_tag="VGB57_02900"
     CDS             complement(1076..>1689)
                     /gene="hrcA"
                     /locus_tag="VGB57_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126640.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0130328.1"
                     /translation="VALSPGRALAVLVGADGSVENRILSLEAGTDPAALNEVANFVNA
                     RLSGMTLAEAEGRLRAEIGQRKKALDRAAAELIASGLASWSEDHSRRPVLIVRGQANL
                     IDERAGEDLERVRKLLDEPEDHQEIARLLEGARDAPGCRIFIGSENRMFALSGSSVIA
                     APYRGSQGQVVGVVGVIGPTRLNYARVVPMVDFTAKALTRLMA"
BASE COUNT          264 a          589 c          544 g          292 t
ORIGIN      
        1 ggccgccgga tcctgccgac ccgccaggaa gaattctatt tcgcagcgcc ggccggcgac
       61 cgacgtttcc tgccaggagc gatgcccggc tgggccgact tcaacggcgg cgacatcttc
      121 tacggcttcc cggacctgga aagccgcggc ctcaagttcg cccacgacca gcatggggtg
      181 gaaatcgatc cggacaccca ggaccgccgg ccgacacagg ccgcgctggc ggagatcatc
      241 gccttccgcg accgccggtt cccgctgctt cgcggggcac ccctgacgga atcgcgcgtc
      301 tgccagtacg aaaacagctc gaacggcgac ttcctgatcg atttccaccc gcggctgccc
      361 aatgtgctgc tggtcggcgg cggctcgggc cacggcttca agcacgggcc agaggtcggc
      421 cgctacgccg cggcgagatt attgggtgga actgcggtcg agccgcgctt cagcctcgcc
      481 accaaggcgg agacccacaa gcgggaagtg cattaggcgt cgggagcttt cgccactccg
      541 accagcgccg gccgaagaag ccggtccttg agcatgtagc cggcctgcat ctcgtcgacg
      601 atcgtcccag gctcggcatt ctcagtcggg atctccatca tcgcctggtg ccggtgcggg
      661 tcgagcttct cgccgaccga ctgaattcgg gtgatgccct ggcgcgtaaa tgccgcttcc
      721 aattcgcgcg cagtcgcctc gatgccggca aggaaattgg cggccgtctc gtcggcgcgc
      781 agctcgtcgg aaaccgcggc cagcgcccgg tcgaggtggt ccttgatcgc cagcatgtcg
      841 cgcgcgaatg cgcttgaagc ataagccgcg gcttggacct tctcggcctc gagccggcgc
      901 cgcacgttct gggtctcggc ggccgcgtac agtgccttgc tggtcgcgtc ctcgagcgcc
      961 ttctcgagtt ccacaacgcg gtcgtgctcg gcgagctcgg gcgccgcctc ggctgtttcc
     1021 ttgcggattt cctcggcctc ttcgtggagg tcttcgtggt cgttcattcc aggtgtcatg
     1081 ccatcaatct cgtcagggcc ttggccgtga aatcaaccat ggggaccacc cgcgcatagt
     1141 tcaaccgcgt ggggccaatc actccgacaa ctcccacgac ctgtccctgg ctcccgcgat
     1201 agggcgcggc gatcaccgac gagcccgaca aagcgaacat ccggttctcc gagccgatga
     1261 agattcggca gccgggagcg tcgcgggcgc cttcgagcag gcgggcgatt tcctggtggt
     1321 cctcgggctc gtcgagcagc ttccgaacgc gctcaagatc ctcgccggcc cgctcgtcga
     1381 tcaaattggc ctggccgcgg acgatcagaa ccgggcggcg tgaatgatcc tcgctccagg
     1441 acgcgagccc ggaggcgatc aattcggcgg cggcgcggtc gagcgccttc ttgcgctggc
     1501 cgatctcggc ccgcagccgc ccctccgcct ccgcgagcgt catccctgac aaccgcgcgt
     1561 tgacgaaatt ggcgacctcg ttgagcgcgg cggggtctgt gccggcttcg agcgaaagga
     1621 tccggttctc gacgcttcca tcggctccga ccagcaccgc gagcgcgcgg cccggcgaca
     1681 aagcgacga
//
LOCUS       DASWNU010000149         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_316454, whole genome shotgun sequence.
ACCESSION   DASWNU010000149 DASWNU010000000
VERSION     DASWNU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316454"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="dnaX"
                     /locus_tag="VGB57_02905"
     CDS             complement(<1..965)
                     /gene="dnaX"
                     /locus_tag="VGB57_02905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0130329.1"
                     /translation="MASESPDDSPGFGLALDEPPQPAKAQPYRVLARKYRPQSFAELI
                     GQDAMVRTLGNAIERDRLAHAFLLTGVRGVGKTSTARLIAKALNCIGPEGQGGPTIAP
                     CGICEPCRAIAEGRHIDVVEMDAASHTGVDDVREIIEAVRYSAVSARFKVYIVDEVHM
                     LSKNAFNALLKTLEEPPQHVKFLFATTEVNKVPVTVLSRCQRFDLRRIPAEKLAAHFG
                     EVAHKEGVEVEPEALALIARAAEGSARDGLSILDQAIAHGPGGVSAEQVRAMLGLSDR
                     GAIRRLLGVILSGDARSALAVLRDQYDLGVEPNAVLRGLLESVHG"
     gene            complement(1075..1173)
                     /gene="ffs"
                     /locus_tag="VGB57_02910"
     ncRNA           complement(1075..1173)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGB57_02910"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1306..2211)
                     /gene="bla"
                     /locus_tag="VGB57_02915"
     CDS             complement(1306..2211)
                     /gene="bla"
                     /locus_tag="VGB57_02915"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEY0130330.1"
                     /translation="MIDRRTFLAASAGAALAAGCRAAAPARDPEPARLAIDARRRFEA
                     IRATLGPGGRLGVAAIDTGSGRELRFDADSRYAMASTFKFPLAGAVLALADQGKLSLE
                     EKLPIPPGKLLDNSPAVERYREEGSLSIVRLCSAIVELSDNSGANMLLRRIGGPEALT
                     RFIRDCGDPVTRLDRYEMELNSNLPGDPRDTTSPAAMAGLARTLVLGDRLSGQSRRHL
                     STWLTKAVPGPDRLKAGLPSPPWLVGHKTGTGANGAVNDVAIAWRSGKPPLVIASYQS
                     GGNAEPRFRAAAHAAVGRLAAETFA"
     gene            complement(2208..2543)
                     /locus_tag="VGB57_02920"
     CDS             complement(2208..2543)
                     /locus_tag="VGB57_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0130331.1"
                     /translation="MTLVRFVSADGNHIQEVRAAPGERLLDLAQAHGQPLEGTCEGQM
                     ACSTCHVVVAAEDFAKLPSASEEEEDLLDLARHVTRTSRLACQIHLSETLENLTVRMP
                     GGFHNMQGR"
     gene            complement(2540..>3476)
                     /locus_tag="VGB57_02925"
     CDS             complement(2540..>3476)
                     /locus_tag="VGB57_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY0130332.1"
                     /translation="KLYFTSGATEAINWALKGVASRLPPGRRRIVTLATEHAAVLDSV
                     EWLARQGFDVELLPVRPDGLVEPEIVKAAVDKPTGLVAAMLVNNEIGVIQPIPAIAAA
                     ARAAGALFFCDAVQGLGRVPLPLADCDMVAVSAHKVHGPKGIGALWVRAGVRLDPLLH
                     GGGQEGGLRSGTLSPALCVGFGEAAQLLMERIQTDSIHARRLWSRARASMPDWTVNGS
                     SEYRYWGNFNLRRGGVDADRLISELRQIAFSTGSACASGSGRPSHVLAALGLSDREAR
                     ASIRLGFGRYTTEAEIAEAAVLINAAANRQLNFAG"
BASE COUNT          549 a         1123 c         1271 g          533 t
ORIGIN      
        1 atgccgtgga cgctctccag caggccgcgg agcacggcat tgggctcgac cccgaggtcg
       61 tattggtcgc gcagcacggc cagcgccgat cgcgcatccc cgctcaatat tacacccagc
      121 agccggcgga tcgcgccgcg gtcggaaagg ccgagcatgg cccgcacctg ctcggcgctg
      181 accccgccgg ggccgtgggc gatcgcctgg tcgaggatgg aaaggccgtc gcgggccgag
      241 ccttcggcgg cgcgggcgat gagcgcgagc gcctccggct cgacctcgac tccttccttg
      301 tgcgccacct cgccgaaatg ggcggcgagc ttctcggccg ggatccggcg aaggtcgaag
      361 cgctggcagc gcgagaggac ggtgaccggg accttgttga cctcggtcgt cgcgaaaaga
      421 aacttaacgt gttgaggggg ttcctccagg gtcttgagaa gcgcgttgaa cgcgttcttg
      481 gacagcatat ggacttcgtc gacgatatag accttgaagc gcgccgagac ggccgaatag
      541 cgcaccgcct cgatgatctc gcggacgtcg tcgactccgg tatggcttgc ggcgtccatc
      601 tcgacgacgt cgatatggcg cccctcggcg atcgcccggc agggctcgca gattccgcag
      661 ggagcgatgg tgggaccgcc ctgcccctcc ggtccgatgc agttcaatgc cttggcgatc
      721 aggcgcgcgg tcgatgtctt gccgaccccg cgaacgccgg tgagcaggaa cgcgtgggcg
      781 agacggtcgc gctcgatcgc gttgccgagc gtccgcacca ttgcgtcctg cccgatcagc
      841 tcggcgaagc tctgcggccg gtattttcgg gcgaggaccc gatagggctg cgccttggcc
      901 ggctggggcg gctcgtcgag ggcgaggccg aagcctgggg agtcgtcggg ggattcggat
      961 gccatggcgg gagactagcc tattccgtca tgccagcgaa ggctggcatc ccggcgggtg
     1021 aagaatgccg cgcttttcac cccccgggcc cccagcctgc gctggggcga cgcgaaagtg
     1081 ggagccggaa acgacccggc gcttgaactc gttacggctg cttccttccg gacctgaccg
     1141 ggttggcgag agcgccgtcc gcccgactcc cgcggcgcat atggtggaag agaggccggg
     1201 acgcaagata ccggccccgt ccgtcatccc ggcgaaagcc ggggcctcag gacaaaaggt
     1261 ctcggcgggc ctccccgagg tcccggcttt cgccgggatg acggcctaag cgaaggtctc
     1321 cgccgccaga cggcccaccg cggcatgggc ggccgcgcga aaccggggct ccgcattgcc
     1381 gccgctctgg tagctggcga tgacgagggg gggcttgccg gagcgccagg cgatggcgac
     1441 gtcgttgaca gcgccgttgg cgccggtgcc ggtcttgtgg ccgaccagcc agggcggcga
     1501 aggcaggccc gccttgagcc ggtcgggacc tggcaccgcc ttggtcagcc aggtggagag
     1561 atgtctgcgc gattgcccgg agagcctgtc gcccagaacc agcgtacgcg ccaggccggc
     1621 catcgccgcc gggctcgtgg tgtcgcgcgg atcgccggga aggttcgagt tgagctccat
     1681 ctcgtagcgg tcgagccggg tgaccggatc gccgcagtcg cggatgaagc gggtcagcgc
     1741 ctccggcccg ccgatccggc gcaggagcat gttggcgccg ctgttgtcgc tcagctcgac
     1801 gatggccgag cagagtcgaa cgatcgagag gcttccctcc tcgcgatatc tttcgaccgc
     1861 gggcgagttg tcgagcagct tgccgggcgg gatggggagc ttctcctcga gcgagagctt
     1921 gccctggtcg gcgagggcga gaaccgcgcc ggcgagcgga aacttgaagg tcgaagccat
     1981 cgcgtagcgc gaatccgcgt cgaagcgcag ctcgcggccg gagccggtgt cgatggccgc
     2041 gactccgagc cggccgcccg ggcccagggt ggcgcgaatc gcctcgaacc gccttcgggc
     2101 gtcgatcgcc agccgggcgg gttcggggtc cctggcgggg gcggcggcgc ggcagccggc
     2161 ggcgagggcg gcgccggcgc ttgcggcgag gaaggttcgg cggtcgatca tcgtccctgc
     2221 atgttatgga agcctccggg catccgcacc gtcagatttt ctaaggtctc gctcaagtgg
     2281 atctggcagg cgaggcgcga ggtgcgggtg acgtgccggg cgagatcgag cagatcctcc
     2341 tcttcctcgc tggcgctcgg aagcttcgcg aaatcctcgg cggcgacgac gacgtggcag
     2401 gtcgagcagg ccatctggcc ctcgcacgtc ccttcgagcg gctggccgtg cgcctgcgcc
     2461 agatcgagca ggcgctcgcc cggagccgcc cggacctcct ggatgtggtt gccgtcggcc
     2521 gagacgaagc ggaccagggt catccggcga agttcagctg gcgattggcg gcggcgttga
     2581 tcagaaccgc ggcctcggcg atctcagcct cggtcgtata gcggccgaag ccgaggcgaa
     2641 tgctggcgcg cgcctcgcgg tcggagaggc cgagggcggc gaggacgtgg ctgggacggc
     2701 cagagccgct tgcgcaggcc gagccggtcg agaaggcgat ctgccgaagt tcggaaataa
     2761 gtcggtcggc gtcgacgccg ccgcggcgca agttgaaatt accccagtac cgatattcgg
     2821 atgagccgtt gacggtccag tccggcatcg acgcccgtgc cctgctccat agccgacggg
     2881 cgtgaatgct gtcggtttgg atcctctcca tcagaagttg cgctgcctct ccgaatccca
     2941 cgcacaaagc gggcgaaagg gtgcccgaac gcaatccgcc ctcctgtccg ccgccgtgaa
     3001 ggagcgggtc cagccggacg ccggcccgga cccaaagggc accgataccc ttggggccat
     3061 gcaccttgtg ggccgagacc gcgaccatgt cgcaatcggc aaggggcagc ggaacgcgtc
     3121 cgagaccttg aacggcgtcg cagaagaaaa gtgcgccagc cgctcgggcc gcggcggcga
     3181 tcgccggaat cggctggatc accccgatct cattgttgac cagcatcgcg gcgacgagac
     3241 cggtgggctt gtcgaccgca gccttgacga tctcgggttc gaccagtccg tcggggcgga
     3301 cgggaaggag ttcgacgtcg aagccttggc gagcaagcca ttcgacagag tcgagcacgg
     3361 ctgcatgctc agtagcgagg gtgacgatcc ggcggcggcc gggcggcagg cgcgaggcaa
     3421 cacccttcaa ggcccagttg attgcctccg tcgcgccgga ggtgaagtag agcttt
//
LOCUS       DASWNU010000150         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_83406, whole genome shotgun sequence.
ACCESSION   DASWNU010000150 DASWNU010000000
VERSION     DASWNU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83406"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2888)
                     /locus_tag="VGB57_02930"
     CDS             complement(<1..>2888)
                     /locus_tag="VGB57_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY0130333.1"
                     /translation="KPIARDYSLVRAAAAPRTQEWDVAEAKSAGCVSCHTDSDRKTMH
                     SSPAVVLGCVDCHGGNAAVAGDSSLAHDSAAYVAARDKAHVLPRYPGSWHWPSSANPS
                     RTYTLLNKESPEYVRFVNPSDYRVARDSCGACHIEVIEAAERSIMASGAMLWGGAAYN
                     NGIVPYKNYIFGEAYTRRGEPAKIVSPPGPQALTPEQKARGVLGELYPLPTWHVVPPG
                     DVFRVFERGGRTIGTQFPEIGLPSPSGSIQRLEESGRPDIRQSNRGPGTGLRVAIPVL
                     NIHKTRLNDPFMWFMGTNDQPGDYRHSGCAGCHVVYANDREPRHSLTYARFGRDGQTG
                     TVDPTIRDKTQATHAPADAAHASLKEKGHPVRHAFTRAIPTAQCMSCHMHQPNIFLNS
                     FLGYTMWDYEADADLMWPGPENRLPAANAAEEAGLRRQRHPDAAQMHEVLDRNPEGAA
                     PRGLWGDLDFLRNVYDRVNPLARHTQFADYHGHGWNFRAVYKRDREGNLLDADGAVVA
                     PDDPEKFRTSGAPEFVPPGVQKGKAVHLMDIHAEKGMQCADCHFAQDNHGNGYIYGEV
                     ANAVEIGCKDCHGTATQYPNLLTSGPAAPPRGTNLALLRNEDGRRRFEWMVNDEGRRV
                     LVQRSIVDPELEWEVSLVKDSLDPGGPGFNLKAARAKLMSSDGAESGRYVFGSGVAPE
                     ELAHKDNEMACFTCHLSWTTSCGGCHLPVEANWKTKSQKYEGIETRNFATYNPQVARD
                     DMFQLGRHQTTKGNVIAPVRSSSALILSSTNINRERIYVQQPPISSAGFSSQAFAPHF
                     PHTVRRTETKMCTDCHLSQLNDNNAVMAQLLLQGTNFVNFVGLNAYVGLDGAFQATRV
                     TEWDEPQAVIGSYLQRFAYPDWWRLHVDRNKRELVNWTRASKGGAEQFANIVQGTGGT
                     VGCLQQRGEYMFVAEGRGGFRVYDIASVGNKGVSDRIVTAPFSPLGHDT"
BASE COUNT          437 a          876 c         1034 g          541 t
ORIGIN      
        1 gtgtcgtggc cgagcgggga gaagggcgcg gtgacgatcc gatccgagac gcccttgttg
       61 ccgacgctgg cgatatcata gacccggaat ccgcctctgc cctcggcgac gaacatatat
      121 tcgccgcgct gctggaggca gccgacggtt ccgccggtgc cctggacgat gttggcgaat
      181 tgctcggcgc cgcctttgga ggcgcgggtc cagttgacca gctcgcgctt gttgcggtcg
      241 acgtggaggc gccaccagtc ggggtaggcg aagcgctgaa ggtaggagcc gatcaccgcc
      301 tgcggctcgt cccattcggt gacccgggtc gcctggaagg cgccgtcgag gccgacatag
      361 gcgttgaggc cgacgaaatt gacgaagttg gtgccctgca gaagcagttg cgccatcacc
      421 gcattattgt cgtttagttg cgataaatgg caatccgtgc acattttcgt ttcagtcctt
      481 cgcaccgtgt gcggaaaatg cggagcgaag gcctgggacg aaaaaccggc ggagctgatc
      541 ggcggctgct ggacgtaaat cctttcgcgg ttgatgttgg tcgagctcag gatcagagcc
      601 gagctcgacc tgaccggtgc tatgacattg cccttggtgg tctggtggcg gccgagctgg
      661 aacatgtcgt cgcgcgcgac ttgtggatta taggtggcga aattgcgggt ctcgatgcct
      721 tcgtatttct ggcttttggt cttccagttc gcctcgaccg gcaagtggca gccgccgcaa
      781 ctcgtcgtcc agctgagatg gcaagtgaag caggccatct cattgtcttt atgcgctaat
      841 tcttccggag cgactccact tccgaaaacg tagcgaccag attccgcccc gtcgctcgac
      901 atcaatttcg cccgcgccgc cttgaggttg aagccggggc cgccggggtc gagcgagtcc
      961 ttcaccagcg aaacttccca ttccagctcg ggatcgacga tcgagcgctg gaccagaacc
     1021 cgccgccctt cgtcgttgac catccactcg aagcgccgcc gtccgtcctc gttgcggagg
     1081 agagcgagat tggtgcccct gggcggagcg gcgggcccgg aggtcagcaa attgggatat
     1141 tgggtggcgg tgccgtggca gtccttgcag ccgatctcga ccgcgttcgc cacctcgccg
     1201 tagatatagc cgttgccgtg attgtcctgg gcgaaatggc agtcggcgca ttgcatcccc
     1261 ttctcggcgt ggatgtccat gaggtggacc gccttgccct tctggacgcc gggcgggacg
     1321 aattcggggg ctccggacgt cctgaacttc tccgggtcgt cgggcgcgac gacggcgccg
     1381 tcggcatcga gcagattgcc ctcgcggtcg cgcttgtaga cggcgcggaa gttccagccg
     1441 tggccgtgat agtcggcgaa ctgggtgtgc ctggcgagcg ggttcacccg gtcgtagacg
     1501 ttgcgcagga agtcgagatc gccccacagg ccgcgcggcg ccgcgccttc gggattgcgg
     1561 tcgagcactt cgtgcatctg cgccgcatcg ggatggcgct ggcggcgaag gccggcctcc
     1621 tccgccgcgt tggccgccgg cagccggttc tccggacccg gccacatcag gtcggcgtcc
     1681 gcctcgtaat cccacatcgt gtagccgagg aaggagttca ggaagatgtt gggctggtgc
     1741 atgtggcagc tcatgcactg ggcggtcggg atggcgcggg tgaaggcgtg gcggacgggg
     1801 tggcccttct ccttcagcga ggcgtgggca gcgtcggcag gggcatgggt cgcctgcgtc
     1861 ttgtcgcgga tggtgggatc caccgtcccc gtctgcccgt cgcggccgaa ccgggcgtag
     1921 gtgaggctgt gcctcggctc gcggtcgttg gcgtagacga cgtggcagcc ggcgcagccc
     1981 gaatggcgat agtcgccggg ctggtcgttg gtgcccatga accacatgaa ggggtcgttg
     2041 aggcgggtct tgtggatgtt gagcacgggg atggcgaccc tgaggccggt gcccgggccg
     2101 cggttcgact gacggatgtc gggccggccg ctctcctcga gcctctggat cgagcccgag
     2161 ggggagggga ggccgatctc ggggaactgg gtgccgatcg tccgtccgcc gcgctcgaag
     2221 acccggaaga cgtcgcccgg cgggacgacg tgccaggtcg gaagcgggta gagttcgccg
     2281 aggacgccgc gtgccttctg ctcgggcgtg agcgcctgcg gacccggcgg cgagacgatc
     2341 ttcgccggtt cgccgcggcg ggtataagcc tcgccgaaga tgtagttttt atagggcacg
     2401 atgccgttgt tgtaggcggc cccgccccag agcatcgcgc ccgaagccat gatcgagcgc
     2461 tccgccgcct cgatcacctc gatatggcag gccccgcaac tgtcgcgcgc cacccgataa
     2521 tcggacggat tgacgaagcg cacatattcc gggctctcct tgttgagcaa ggtgtaggtg
     2581 cggctcggat tggcgctgga cggccaatgc caggatccgg ggtagcgggg gaggacgtgc
     2641 gccttgtccc gcgcggcgac ataggcagcg ctgtcgtggg cgagcgagga gtcgccggcg
     2701 accgccgcat tgccgccgtg gcagtcgacg cagccgagca ccacggccgg gctcgaatgc
     2761 atggtcttcc tgtcgctgtc ggtgtggcag gagacgcagc cggcgctctt cgcctcggcg
     2821 acgtcccatt cctgcgtgcg cggcgccgcc gcggctcgga cgagggaata gtcgcgagct
     2881 atgggctt
//
LOCUS       DASWNU010000151         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_394412, whole genome shotgun sequence.
ACCESSION   DASWNU010000151 DASWNU010000000
VERSION     DASWNU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394412"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..429)
                     /locus_tag="VGB57_02935"
     CDS             complement(76..429)
                     /locus_tag="VGB57_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130334.1"
                     /translation="MAWTENTVICTYRVREGARDAFVELLKKHWPALRGAGLVTDTPA
                     VHFEAAPSKGDPHAESGTTFIEIFSWAGPGMADRAHELPEVMAVWEPMGALVEARGGR
                     PPMEFPLVRPLDLGT"
     gene            709..912
                     /locus_tag="VGB57_02940"
     CDS             709..912
                     /locus_tag="VGB57_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130335.1"
                     /translation="MAEDREKITTGPNERAVNETIAGTGPGIPDDARAPGEELPEPPS
                     DEEVARAAQRLGAQLHVDKGGNG"
     gene            981..1065
                     /locus_tag="VGB57_02945"
     tRNA            981..1065
                     /locus_tag="VGB57_02945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1015..1017,aa:Leu,seq:caa)
     gene            complement(1168..>1654)
                     /locus_tag="VGB57_02950"
     CDS             complement(1168..>1654)
                     /locus_tag="VGB57_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY0130336.1"
                     /translation="GETFDVRPVAARGPRPIPRGLTLSYMILPLKRYFDFSGRSRRKE
                     YWSYFLFLVIALIVMMLLDTKLGLGGTTDTYAEYGDAGASAGVKMTGGLLTLLFALAT
                     LIPSIAVAVRRAHDQDRSGWFILIPIYNLIMMFIEGTRGPNRFGPDPKAGDAEGRAAA
                     A"
BASE COUNT          280 a          554 c          574 g          246 t
ORIGIN      
        1 gacgggtggg cgagcgccgc gaagacgcgg tcggcctgct ccagccgcgc cttggggtca
       61 ggccccccgc tgtccttatg tgccaagatc gagcgggcgg acgagcggga actccatcgg
      121 cggccggccg ccccgcgcct cgaccagggc gcccatcggc tcccacaccg ccatcacctc
      181 gggcagctcg tgcgcccggt cggccatgcc cgggcccgcc cagctgaaga tctcgatgaa
      241 ggtcgtgccc gattcggcgt gcgggtcgcc cttcgatggg gcagcctcga agtgcacggc
      301 cggcgtgtcg gtgacgagcc cggccccgcg cagggccggc cagtgcttct tcagcagctc
      361 cacgaacgcg tcgcgcgcgc cttccctgac gcggtaggtg cagatgacgg tgttctcggt
      421 ccacgccatg acaattctcc tgctcgggag gaggttactt agcaggatcg ctaaatgagt
      481 ccagcctggg cggctgcgag gaaaaacggg gacagtatac attttccccc ggagctcttt
      541 cacgcgagtg cgggggtgag gaaaatgtat actgtccccg ttttctggcc ctgccgctgg
      601 ccgcacaggc gcaatccctc ccctgcgcca gtgcagcaat gagcggcgcc cgccgggaac
      661 cgcgacgcgg cccgctcgct tgttgccgcg agcgacagga gcgacgccat ggcagaggac
      721 agagagaaga tcaccaccgg ccccaacgag cgcgcggtca acgagacgat cgccggcacc
      781 ggccccggca tccccgacga cgcccgggcg cccggcgagg agctgcccga gccgccgagc
      841 gacgaggaag tggcacgggc ggcgcagcgc ctcggcgcgc agcttcacgt agacaaaggc
      901 ggcaacggct agcgcagccg tcgtcgctca ggcggacggt tgcggcccgc ctggcacttc
      961 agtagggcgg gcgctcgcct gcccccgtgg cggaattggt agacgcgctc gactcaaaat
     1021 cgagttccgc aaggagtgcc ggttcgactc cggccggggg caccagccac accgtcattg
     1081 cgagcgaagc gaagcaatcc agagcggctt ctgacgcagt ctggattgct tcgtcgcttc
     1141 gctcctcgca atgacgcggc gtgaggacta ggcggccgcg gcccggccct ctgcgtcgcc
     1201 cgccttgggg tcggggccga agcgatttgg gccgcgggtc ccttcgatga acatcatgat
     1261 gagattgtag atcgggatca ggatgaacca gcccgatctg tcctggtcgt gggcgcggcg
     1321 gaccgccacg gcgatgctcg ggatcagggt ggccagcgcg aagaggagcg tgaggaggcc
     1381 gccggtcatc ttgacgcccg ccgaggcgcc ggcgtcgcca tattcggcat aggtgtcggt
     1441 ggtgccgccg aggcccagct tggtgtcgag cagcatcatg acgatcagcg cgatgacgag
     1501 aaacaggaaa taggaccaat attccttgcg ccgcgagcgg ccggaaaagt cgaaataccg
     1561 cttcagcggc aggatcatgt agctcaaggt cagccccctc gggatcggcc gcggccccct
     1621 tgccgcgacc gggcgaacgt cgaacgtttc gcct
//
LOCUS       DASWNU010000152         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_421332, whole genome shotgun sequence.
ACCESSION   DASWNU010000152 DASWNU010000000
VERSION     DASWNU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421332"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..983
                     /locus_tag="VGB57_02955"
     CDS             138..983
                     /locus_tag="VGB57_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130337.1"
                     /translation="MSSSRHRFVLRSCACLIALVAVVALVACGDSEDSQELSFTVESN
                     GKAAKVTAPESAEAGLAEVTLTNDSDSAADLQLVRVEGERAAEEVVKGLGEAIQGKSF
                     PDWLFTAGGVGSVEPGESATVTQVLQPGDYYAFNTSGGPPDPKAAGVTEVTGETSGEE
                     IEGGEATVEAAEYVFNTETLPAGATEIAFDNIGAQPHHMIASPLKGNATAEDVEKFFK
                     TEQGAPPLEDKGTKSTAVLEGGEGQLVTMDLKPGRYALYCFITDREGGPPHVFKGMVD
                     EVEVK"
     gene            complement(1008..>1869)
                     /locus_tag="VGB57_02960"
     CDS             complement(1008..>1869)
                     /locus_tag="VGB57_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0130338.1"
                     /translation="AGHVVALGEREDLDPGDVALASDPGYPVYTAGPVLAGAEAELLP
                     LVPELGFAPDLSAVPADVAARARLMFVNYPNNPTGAVVPDGFFEQVVEFAREHEILVV
                     HDNAYSETTYDGYVAPSFLATPGAKEVGVEVFSLSKGYNMTGWRCAAILGNAEAIQTY
                     WRLKTNVDSGLFEAIQMAGVAALEGPDEPLEQMNQTYTRRRDLVVSALREIGVEVSAP
                     KGTIYVWAPVPESHTSTSFCELVLEQAAVVVSPGSMYGPSGEGFFRIALTAPDDRIEE
                     AVERMREHLS"
BASE COUNT          307 a          618 c          680 g          264 t
ORIGIN      
        1 gttcccaccg cctaccagtg gggcggcctc gatcggtact ggcgcaagaa ggcggaagcg
       61 gagaagcccg cacaggcttg aggaccgacc gtcgcgttag gttcttccgg actcgtcccg
      121 cgtcctagga ggttcccttg agcagctccc ggcatcgttt cgtcctgcgc agctgcgcct
      181 gcctgatcgc cctggtcgcc gtcgtggccc ttgtcgcctg cggggactcg gaggactccc
      241 aggagctcag cttcacggtc gagtcgaacg gcaaagcggc gaaggtgacg gcgccggaga
      301 gcgccgaggc gggcctcgcc gaggtcacgc tgaccaacga cagcgactcg gcggccgacc
      361 tgcagctggt ccgggtcgag ggcgagcgcg cggccgagga ggtcgtcaaa gggctgggcg
      421 aagcgatcca gggcaaatcg ttccccgact ggctcttcac cgccggcggc gtcggctccg
      481 tcgagcccgg tgagagcgcc accgtgacgc aggtgctgca gcccggcgac tactacgcct
      541 tcaacacctc cgggggcccg ccggacccga aagccgccgg cgtcaccgag gtcaccggcg
      601 agacgtccgg cgaggagatc gagggcggcg aggccacggt cgaggcggcc gagtacgtct
      661 tcaacaccga gacgctgccc gccggggcga ccgagatcgc cttcgacaac atcggcgccc
      721 agccccacca catgatcgcc tcgccgctga aaggcaacgc caccgccgag gacgtggaga
      781 agttcttcaa aaccgaacag ggcgcgccgc cgctggaaga caaaggcacc aagtcaaccg
      841 cggtgctcga gggcggcgaa ggccagctgg tgacgatgga cctgaagccg ggccgctacg
      901 ccctctactg cttcatcacc gaccgcgaag gcggcccccc gcacgtcttc aaaggaatgg
      961 tcgacgaagt cgaggtgaag tagctgccgc gctgcgggtg cctcggctca gctgaggtgc
     1021 tcgcgcatgc gctcgacggc ctcttcgatc cggtcgtcgg gggcggtcag cgcgatgcgg
     1081 aagaagccct cgccgctcgg accgtacatc gagccgggtg agacgacgac ggccgcctgc
     1141 tcgagcacca gctcgcagaa ggaggtcgag gtgtggctct cggggacggg cgcccagacg
     1201 tagatcgtcc ccttcggggc gctgacctcg acgccgatct cgcgcagggc ggagacgacg
     1261 aggtcgcggc ggcgggtgta ggtctggttc atctgctcca gcggctcgtc gggaccctcg
     1321 agggcggcga cgccggccat ctggatcgcc tcgaagaggc cggagtcgac gttggtcttc
     1381 agccgccagt aggtctggat cgcctctgcg ttgccgagga tcgcggcgca gcgccagccg
     1441 gtcatgttgt agcccttgga gagcgagaag acctcgaccc cgacctcctt ggcgccgggc
     1501 gtggccagga agctgggcgc gacgtagccg tcgtaggtcg tctccgagta ggcgttgtcg
     1561 tggacgacga ggatctcgtg ctcgcgggcg aactcgacga cctgctcgaa gaacccgtcg
     1621 gggacgaccg cgccggtggg gttgttcggg tagttgacga acatcagccg ggcgcgggcg
     1681 gcgacgtcgg cggggaccgc gctcaggtca ggcgcgaagc ccagctccgg gaccaacggc
     1741 agcagctccg cctcggcgcc ggcgaggacc ggtcccgcgg tgtagacggg gtagccggga
     1801 tcggaggcga gggcgacgtc gccggggtcg aggtcttctc gctctccaag ggctacaaca
     1861 tgaccggct
//
LOCUS       DASWNU010000153        12479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_6181,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000153 DASWNU010000000
VERSION     DASWNU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12479
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6181"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2031
                     /locus_tag="VGB57_02965"
     CDS             58..2031
                     /locus_tag="VGB57_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0130339.1"
                     /translation="MLQVDAGRGVAAALAALLLCACPGTLLAQEQPPPGDPLDQPPLD
                     PSAPLDPLPDLGVDWPDMEQGADDRIADAPDTGIADAAAERGYTVRIEGLDADTEAAL
                     REPFDSLSTLAKNVKDPANAAQIDRRAREDADLLAELLRARGYYDALVRTRVEAAPAG
                     GDVVVTLEAEPGPVYRFAEVKLPGIEAAGEDMAALREAFAVAANEPVDAARVTTGEAA
                     LRVELGRRGYAFAETGEMDIALDHETRTAILTLPVNPNGSRQFGRIAVEGRPLFSASH
                     IQRIARWKPGAPYTAALVEDLRRALIATGLVSTVAIRPVAAQDKSVDLVVSLERAPMR
                     TVAGQAGYGTGEGIRVEASWQHRNLLPPEGAVTFRGVIGTQEQLVGAVLRRNNFLRRD
                     QVLTAQVAISHIDRAAYDARTTILSGAIERQTNLIWQKKWTWSAGAELVATDEEDVDL
                     ASGMTRRRTFFIGALPASLSYDGSNDLLDPTVGYRLAFRLSPEASLQSGTFGYGRTQV
                     DASVYQPFTPTVTLAARIRLGTILGADRDRIAPSRRFYAGGGGSVRGYGFQRLGPRDP
                     VFDDPIGGRSLTEFAVEARIRWGNWGVVPFLDGGNISTSPLPKFDNLRFGAGIGLRYH
                     TRFGPIRVDVGTPLNPRSGDSRVAVYVSLGQAF"
     gene            2031..6209
                     /locus_tag="VGB57_02970"
     CDS             2031..6209
                     /locus_tag="VGB57_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0130340.1"
                     /translation="MDEAEAPPPPRRRRRWLSRLAKWVVGIALTLALLTALLAVLLDT
                     GAGHRFIADRIAAMAPRSGLRIHIGRIEGSIWSDTRLRDVRLYDPKGLFAESPLIEVD
                     WQPLGWITNRLLIHRLEADLAALHRLPRMRPSERPGPVLPGFDIHIGRLDVAQLRIGK
                     AVTGQPRVATLAGEADVRSGRALVNLKAAVKDGGDRLSLFLDAEPDRDRFDLDVRLAS
                     PAGGVAGAMLGTGRPIALTVDGDGSWARWNGTARLDLSGRRTADLRLRAENGRYSLAG
                     RLTPAQFWKGRKARLTAPVVQVAGNATLDDRQLEGRLALRSPALSVVGTGIIDLARSR
                     FRDLRIGADLLRPPALFPNMTGQKVRLVALLNGPFRTFAFAYRLTSPRIAFDKTGFED
                     VWAQGRGRFSKAPVTVPVVMSARRVTGVGDVAGGILANLRVEGVLKVTGKALTGEGLT
                     LTSDKLKGKLSLFVDLVTGRFDVVLSGGLTRYLIPGLGIVDVVTELKVVRHPSGKGTL
                     VTGKGRAWVRRFDNRFLAGLAGGLPYVEADLVRGNDFVLHFRNLVLTAPRIRITGTGF
                     RRRDGTFFFEGKGAQATYGAFAMSLDGRIERPKLAIRLARPNEALGLAEMLLNLDPTP
                     AGYAYRAAGRSHLGPFTSNGAILLPRGQPALIQVAAINVSNTRASGALRSDPGGFTGQ
                     LNVDGGGLAGRLVFSPFNNLQRIAIDLKADNARFASPPPIVIRSGRLEGVALLDPAGT
                     SLKGTLTARGLSRGALSIASLNASGELRGGTGQIRARIAGTRGRDFVFDTVADVAPGR
                     LRLTGRGTVDRRAIVLSEPALLTREGDGWRLASTALTFAGGNATVSGLFGGSTSELNA
                     RVQSMPLGVLDIGWPQLGLGGSASGTLSYRFGAVGEPSGDANLRIRGLTRSGLVLSSR
                     PVDIGVVARLAGGNAALRAVAVSEGRTIGRAQARLAPIGRSGNIGERLSVAPLFAQLR
                     YNGPADTLWRLTGVELLDVSGPVAVGADARGTLANPQIRGSLRTERARIESAVTGMVI
                     DNVRASGRFGGSRLVLDSFAGTTKRTGTVTGRAVFDLAGPKGFGIDIALQAKAAQLLD
                     RDDIKAQVTGPVAIRSDGETGSISGNVELVSGSFRLGSVTASTQVPRLAVREINRPAD
                     ERPVARRLSPWRLDLDVDARNRLMVTGLGINSEWSAGLKIAGTVTEPRISGRADLIRG
                     SYDFAGRRFDLERGQIRFLGESPVNPVLDITAEGGVQGLNAVIRVTGRGQRPEISFTS
                     TPPLPQDELLSRLLFGTSITNLSAPEALQLAAAVASLNNSGGGLDPINAVRAATGLDR
                     LRIVPADITTGQGTAIAAGKYLGRRVYVEVVTDARGYSATLVEYQITRWLSILSSIST
                     IGRESVNVRVSKDY"
     gene            6414..6851
                     /locus_tag="VGB57_02975"
     CDS             6414..6851
                     /locus_tag="VGB57_02975"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0130341.1"
                     /translation="MSSDEDNGFDSGRQSERLPLDAETRLRPNSWSSLQIKMLDLSTS
                     GFRAECDAKVKPGGSVSLDVPGIGSVDAQVEWQRGDQFGARFFAPIELKACQWAFPDR
                     HQTLARLLVERAAAKRSGRRGAESQIRREILSALPIRKGCASA"
     gene            6953..9622
                     /locus_tag="VGB57_02980"
     CDS             6953..9622
                     /locus_tag="VGB57_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0130342.1"
                     /translation="MRKGRFAGLSDKGPEAVTAPELAELLLFDFTPAADEDDHRAARE
                     RARTVQLSLPLLAGSHALWSAVLIGGLAASGATSGLAGLAGLLLAILLLDGALWLGLG
                     RLKLRPYQAMRLTSLHGAATAALWLAAALLVAPEAQTLLVKAALVAGAGSAIAVFFTV
                     PLLMILAVCTTFLVATSMPSKEPVLAIGGAFGLFLLCLGVARSRQFLFAARQRLALEW
                     QAEKARRFVADFEASGRGWFWETNADGALTYVSEPLAAHLGLAAPELIGRRFEDVLLL
                     DSGDGDDSRRSLGFHLSARFPFADAVVRAPTGDDVWWSLSGSPNFDAYGRFLGFRGLG
                     SNLSEQRRSEAEASKLARYDSLTNLPNRAMMRSMLDQALANADARRRGCALMVIDLDR
                     FKQVNDTLGHPVGDKLLKKVAARLTEVLGEDGQVGRLGGDEFEAILPGIEEEGRLSEL
                     AKRLIEQVSRPYKIEGHEVRIGTSVGIAVAVPGKTLAEGLIKDADVALYAAKADGRGT
                     FRFFAQEMHALATERQILEKDLREALAGDQLTLVYQPIVDSVSEEVVAFEALLRWHHP
                     ARGLLPPALVIPLAEETGLMPRIGDWVLRTACAQAAKWPRHIRVAVNLSPVQFADPAL
                     AATVTGTLAASQLDPERLELEINEAVFLADRKVTEATLTRLRGLGVRLALDNFGTGHS
                     GLGHLRDAPLDKIKIDQSFVRGATAPGSRNGAIVRAIVVLAESLGMDTTAEGAETIEE
                     LSLIRRLGCSQVQGFIFARPMPAEEALALATESRPSAEVVGFSRPPRHRLIRNGRLLW
                     QGRTLPVRLRNISTGGAMIECDSELEPGTPIELDLAEAGTLPAEVRWCQQGQVGLHFG
                     EEFALGKLARTRRKGAAPQALKPEYPEPRKAAK"
     gene            complement(9721..11133)
                     /gene="glnA"
                     /locus_tag="VGB57_02985"
     CDS             complement(9721..11133)
                     /gene="glnA"
                     /locus_tag="VGB57_02985"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY0130343.1"
                     /translation="MASDAGKVLKRIEDEEIEWVDLRFTDPRGKWQHLSMVAGAVDED
                     QLSDGFMFDGSSINGWKAINESDMILKPDLDAVYVDPFSATPMLVVFCDIVDPGTGEL
                     YARDPRSTAKRAENYLKGTGVGDTVFVGPEAEFFMFDDVRFEDGYNASYFRIDDIELP
                     TNSGREYEGGNLAHRPRAKGGYFPVGPVDSATDIRGEMVSTMLEMGLPMDKHHHEVAA
                     AQHELGLTYGTLVQTADRMQIYKYVVHMVAQAYGKTATFMPKPIKSDNGSGMHTHFSI
                     WNGGKPLFAGNGYAGLSEMALYFIGGVIRHAKALNAFTNPTTNSYKRLVPGFEAPVLL
                     AYSSRNRSASCRIPYGAGEKAKRVEFRFPDALANPYLAYSAILMAGLDGIQNRIHPGD
                     PMDKNLYDLPPQELVNVPTVCGSLREALISLQNDRAFLTKGDVFSDDQIDAYMELKWE
                     EQMRWETTPSPVEYDLYYSS"
     gene            complement(11252..11461)
                     /locus_tag="VGB57_02990"
     CDS             complement(11252..11461)
                     /locus_tag="VGB57_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0130344.1"
                     /translation="MDDPRLASMSPEELRAAMRTLGYRTQNDLAAAIGVSRSAVSLWL
                     EGKVGVPRPVAMLLRMLIAAQRRHF"
     gene            11561..>12479
                     /locus_tag="VGB57_02995"
     CDS             11561..>12479
                     /locus_tag="VGB57_02995"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEY0130345.1"
                     /translation="MLLLAAALAAQAPGPSFPCERASAPQEKLVCSRRTLAKADLEMD
                     SAYRGALAKLNPAGRRQLRLSQNAWLRLRNAVCPLGRRDPPNRQSTETCLQARYEERT
                     HQLNEAVRRFGPFRVVRLDGYEVWPAPERDAWYDTVVTNEERFDWIDVDAAPAPLRPA
                     ARTWNAGLDRRPTTAGARWRLHGRGRVQTDDGSNDGPDVDTGRNLEIIAASPDAIVSR
                     EEDYWMGSTHPEENISYETLLIRERRAMRFSDLLTRPAEWGRFAKKRVMSIPPMNTVK
                     DWPRLAETATDPGSLLPGPAGLTVNFGRPL"
BASE COUNT         1881 a         4406 c         4263 g         1929 t
ORIGIN      
        1 ccacaccccg caatcttacg gctggcggcg cccactcgcg gcggctagat tcgtccgatg
       61 ttacaagtcg atgcggggcg gggggtggcg gccgcgctgg ccgcgctttt gctttgcgcc
      121 tgtcccggga cgctgctcgc ccaggagcag ccgccgcccg gggacccgct cgatcagccg
      181 ccgctcgatc cgtccgctcc gctcgacccg ctgcccgacc tcggggtcga ctggccggac
      241 atggagcagg gcgcggacga tcgcatcgcc gacgctcccg ataccggcat cgccgacgcg
      301 gccgccgagc gcggctatac ggtccggatc gaggggctcg acgccgacac cgaggcggcg
      361 cttcgggagc cgttcgactc gctctcgacg ctggccaaga acgtcaagga ccccgccaac
      421 gccgcccaga tcgaccgccg cgcgcgcgag gatgccgacc tcctcgccga gctgctccgc
      481 gcccgcggct attacgacgc gctggtccgg acccgagtcg aagcggcgcc ggccggcggc
      541 gacgtggtgg tcaccctcga ggccgaaccg ggccccgtct accgtttcgc cgaggtgaag
      601 ctgcccggga tcgaagcggc cggcgaggac atggcggcgc ttcgggaagc cttcgcagtg
      661 gcggcgaacg agccggtcga cgcggctcgg gtgacgacgg gcgaggcggc gcttcgggtg
      721 gagctcggca ggcgcggcta cgctttcgcc gaaacaggcg agatggacat cgccctcgac
      781 cacgagacgc ggaccgcgat ccttacttta ccggtcaatc cgaacgggag ccggcagttc
      841 ggccggatcg cggtcgaggg ccggccgctg ttcagcgcca gccacatcca gcgcatcgcc
      901 cgctggaagc cgggcgcgcc ttacacggcc gctctggtcg aggatctgcg gcgggcgctg
      961 atcgcgacgg ggctcgtctc cacggtcgcg atccgtccgg tggcggcgca agacaagagc
     1021 gtcgacctcg tcgtctcgct cgagcgggcg ccgatgcgca ccgtcgcggg acaggccggt
     1081 tatggaaccg gcgaggggat cagggtcgag gcgagctggc agcaccgcaa tttgctcccg
     1141 cccgagggcg cggtgacgtt ccgcggcgtc atcgggactc aggagcagct ggtcggggcg
     1201 gtcctcagac gcaacaactt ccttcgccgc gaccaggtgc tgaccgccca ggtcgcgatc
     1261 agccatatcg accgcgccgc ctacgatgcc cgaaccacga tcctctccgg cgcgatcgag
     1321 cggcagacca acctcatatg gcagaagaaa tggacctggt cggccggagc cgagctggtc
     1381 gccacggacg aggaggatgt cgacctggcg agcggaatga cccgccgccg caccttcttc
     1441 atcggcgcct tgcccgcgag cctcagctac gacggctcga acgacctgct cgacccgacc
     1501 gtcggctacc gcctcgcctt ccggctttcg cccgaggctt cgctccagtc cggcaccttc
     1561 ggctatggcc gaacccaggt ggacgccagc gtctaccagc cgttcacgcc gaccgtgacc
     1621 cttgcggcac ggatccggct cggcaccatc ctcggggccg accgcgaccg gatcgcaccg
     1681 tcgcggcgct tctatgcggg cggcggcggc tcggtgcgag gctacggctt ccagcggctc
     1741 ggcccgcgcg acccggtgtt cgacgatccg atcggcggca ggagcctcac cgagttcgcg
     1801 gtcgaggccc gcatacgctg gggcaattgg ggcgtcgtgc ccttcctcga cggcggcaac
     1861 atctcgacct cgcctttgcc caagttcgac aaccttcgct tcggcgccgg gatcgggctt
     1921 cgctaccaca cccgtttcgg cccgatccgg gtcgatgtcg gaacgcctct caatccgcgc
     1981 agcggcgaca gccgggtggc ggtctacgtc tcgctcggcc aggccttctg atggacgagg
     2041 ccgaggcccc gccgccgccg cgccgccgcc gccgctggct gtccaggctg gccaagtggg
     2101 tggtcggcat cgccttgacc ctcgcgctcc tcaccgctct tctggcggtg cttctcgaca
     2161 ccggcgcagg ccaccgcttc atcgccgacc ggatcgccgc gatggcgccc cgatcgggcc
     2221 tcaggatcca catcggccgg atcgaaggct cgatctggag cgacacccgg cttcgcgacg
     2281 tccggctcta cgatccgaag ggcctgttcg cggaatcgcc cttgatcgag gtcgactggc
     2341 agccgctcgg ctggatcacc aaccgcctgc tcatccaccg cctcgaggcc gatctcgccg
     2401 ctcttcaccg cctgcccagg atgaggccca gcgagcggcc gggaccggtc cttccgggct
     2461 tcgacatcca catcggacgt cttgacgtcg cccagctcag gatcggcaag gccgtcaccg
     2521 ggcagccgcg cgtagcgacc ctcgccgggg aggcggatgt ccgctcgggg cgcgcgctcg
     2581 tcaatctgaa ggcggcggtg aaggacggcg gcgaccggct gtcgctcttc ctcgacgcgg
     2641 agcccgaccg cgaccgcttc gacctcgacg tcaggctcgc ctcgccggcc ggcggagtcg
     2701 ccggggcgat gctcgggacc ggccggccca tcgccctcac cgtcgacggc gacggcagct
     2761 gggcgcggtg gaacgggacc gcccggctgg acctttccgg acggcgcacc gccgacctca
     2821 ggctgcgcgc cgagaacggc cgctacagcc tcgccggccg gctcaccccg gcccaattct
     2881 ggaagggcag gaaagcgcgc ctgacggccc cggtggtcca ggtagccggc aacgccacgc
     2941 tcgatgaccg ccagctcgaa ggacggctgg cgcttcgctc gccggcgctg agcgtggtcg
     3001 gtacgggcat catcgatctc gcccgaagcc gcttccgcga cctccgcatc ggtgccgacc
     3061 tgcttcggcc gcccgctttg ttcccgaaca tgaccgggca gaaggtgcgg ctggtcgccc
     3121 ttctcaacgg cccgttcagg actttcgcct tcgcctatcg gctgaccagc ccgaggatcg
     3181 ccttcgacaa gacgggcttc gaggacgtct gggcgcaggg gcggggccgt ttctcgaaag
     3241 cgcccgtgac ggtgcccgtc gtcatgtcgg cgcggcgggt gaccggggtc ggcgacgtcg
     3301 ccggcgggat cctcgccaac cttcgggtcg agggcgtgct caaggtgacc ggcaaggcgt
     3361 tgaccggcga gggtctgacc ctgacgtctg acaaattgaa gggcaagctc agcctcttcg
     3421 tcgacctcgt aaccggccgc ttcgacgtcg tcctctcggg cggcctcacc cgctacctca
     3481 tcccgggcct ggggatcgtc gacgtcgtca cggagctcaa ggtggtccgg caccccagcg
     3541 gcaagggcac gctcgtcacc ggcaagggcc gcgcctgggt gcgacggttc gacaacaggt
     3601 tcctcgccgg cctggccggc ggcctcccct atgtcgaggc cgatctcgtc cggggaaacg
     3661 atttcgtcct ccatttccga aatctcgtgc tcaccgcgcc gaggatccgg ataactggaa
     3721 ccggcttccg gcggcgcgac ggcaccttct tcttcgaggg caagggggcg caggcgacct
     3781 acggcgcgtt cgcgatgagc ctcgacggcc ggatcgagcg gccgaagctc gcaatccggc
     3841 tcgcccggcc gaacgaggcg ctcggccttg ccgaaatgct cctcaacctc gatccgacgc
     3901 ccgccggcta cgcctaccgg gcagcggggc ggtcgcatct cggccccttc acctcgaacg
     3961 gagcgatcct tctgcccagg ggccagccgg cgctgatcca ggtggcggcg atcaacgtct
     4021 cgaacaccag ggcctcgggg gcgctgcgct ccgaccccgg cgggttcacc gggcagctca
     4081 acgtcgacgg gggcgggctc gccgggcggc tcgtgttcag cccgttcaac aacctccagc
     4141 gcatcgccat cgacctcaag gccgataatg cccgcttcgc cagcccgccg ccgatcgtca
     4201 tccggagcgg ccggctggag ggcgtcgcac tgctcgaccc ggcgggaacc tcgctcaaag
     4261 gcaccctgac cgcccgcggc ctgagccgcg gggcgctctc catcgccagc ctcaacgcca
     4321 gcggcgagct tcgcggcggc accggccaga tcagggcccg gatcgccggc acccggggcc
     4381 gcgacttcgt cttcgacacc gtcgccgacg tcgccccggg ccggctccgg ctgaccgggc
     4441 gcggcacggt cgaccggcgg gcgatcgtgc tgagcgagcc ggccttgctc acccgggaag
     4501 gcgacggctg gcgcctcgcc tccaccgcgc tcaccttcgc gggcggcaac gccacggtct
     4561 cgggcctgtt cggcggatcg accagcgagc taaacgcccg ggtccagtcg atgccgctcg
     4621 gcgtgctcga catcggctgg ccgcagctcg ggctcggagg aagcgcgtcc ggcaccttga
     4681 gctaccggtt cggagcggtc ggggagccga gcggagacgc caatctgagg atccggggcc
     4741 tgacccgatc cggcctcgtc ctctcgtccc ggccggtcga catcggcgtc gtcgccaggc
     4801 tggccggagg caatgccgcg cttcgagccg tcgcggtcag cgaaggccgg acgatcggcc
     4861 gggcccaggc ccgtctcgct ccgatcggga ggagcggcaa tatcggcgag cgcctcagcg
     4921 tcgcgcccct cttcgcccag cttcgctaca acggccccgc cgatacgctc tggcggctga
     4981 ccggggtcga gctactcgac gtctccggcc cggtcgcggt cggcgccgac gctcgcggca
     5041 ccctcgccaa cccgcaaatc cgcggctcgc tcaggaccga gcgggcacgg atcgagagcg
     5101 cggtgaccgg aatggtgatc gacaatgtcc gggcctcggg gcggtttgga ggctccaggc
     5161 tggtcctcga cagcttcgcc gggaccacga agcggaccgg aacggtcacc ggccgggccg
     5221 ttttcgacct cgccgggccc aagggcttcg ggatcgacat cgcgctccag gccaaggccg
     5281 cgcaattgct cgaccgggac gacatcaagg cgcaggtgac ggggccggtc gcgatccgct
     5341 cggacggcga gactggaagc atctcgggca atgtcgagct ggtcagcggc agcttccggc
     5401 tcggctcggt caccgcctcg acccaggtgc cgcgcctcgc cgtccgcgag atcaaccgcc
     5461 ccgccgacga gcggccggtg gcgcggcggc tttcgccctg gcggctggac ctcgacgtcg
     5521 atgcccgcaa ccggctgatg gtgaccgggc tcgggatcaa cagcgaatgg agcgccggcc
     5581 tcaagatcgc cggaaccgtc acggagccca ggatcagcgg ccgcgccgac ctcatccgag
     5641 gcagctacga cttcgccggc cgccgcttcg atctcgagcg cggccagatc cgcttcctcg
     5701 gcgaatcccc ggtcaacccg gtgctcgaca tcacggccga gggcggggtg cagggcctga
     5761 acgcggtgat ccgggtcacc ggccggggcc agcggcccga gataagcttc accagcactc
     5821 cgccgctgcc gcaggacgaa ttgctcagcc gcctcctgtt cggcacttcg atcacgaacc
     5881 tgtcggcgcc cgaagcgcta cagctcgccg cggccgtcgc ctccttgaac aatagcggcg
     5941 gcggcctcga tccgatcaac gcggtgcgcg ccgccaccgg cctcgaccgg ctccggatcg
     6001 tccccgcgga catcaccacc ggccagggca cggcgatcgc ggccggcaag tatctcggcc
     6061 ggcgagtcta tgtcgaggtg gtgaccgacg cccgcggcta ctcggcgacg ctggtcgaat
     6121 atcagatcac ccgctggctc tcgatcctgt cgagcatctc caccatcgga cgggagagcg
     6181 tcaacgtgag agtgtcgaag gattattgac ggcgcctgcc gccgtcattg cgaggagcga
     6241 agcgacgaag caatccagcg ggcggaggcg cttcactgga ttgcttcgct acgctcgcaa
     6301 tgacgccgcg ctaattgatc catgtttaca actactccag ccgcgagaca gcaattcgtt
     6361 aacgcgcagg gtttaatgct ctccccagcc tcaacttggg gatggggatc cgaatgtcct
     6421 cggacgaaga taacggcttc gacagcggcc gtcagtcgga acggcttccg ctcgacgcgg
     6481 aaacccggct ccggccgaac agctggagca gcctgcagat aaagatgctg gacctgtcga
     6541 cgtccggctt ccgcgcggaa tgcgacgcga aggtgaagcc tggcggaagc gtttccctgg
     6601 atgtaccggg catcggctcg gtcgatgccc aggtggaatg gcagcgcggc gatcagttcg
     6661 gagcgcgctt cttcgctccg atcgagctca aggcctgtca gtgggccttc ccggaccggc
     6721 accagacgct ggcccgactg ctggtcgagc gggccgctgc gaagcggtcc gggcgacgcg
     6781 gcgccgaaag ccagataagg cgcgaaatcc tctcggcgtt accgatacgc aagggttgcg
     6841 cgtcggcctg aatcggaggg cgcgataagc gctttcttaa cctcggttac ctagaattcc
     6901 cctcaccggt tccgggggga accggcggat cactatcggg acgcgacaag cgatgcgcaa
     6961 agggcgattc gccggtttga gcgacaaggg cccggaggcc gtcactgcgc cggagctcgc
     7021 ggagctgctg ctgttcgact tcacccccgc cgccgacgaa gacgaccacc gcgccgcccg
     7081 cgaacgggcc cgaaccgtcc agctctcctt gccgcttctt gccggctccc acgccttgtg
     7141 gagcgcggtc ctgatcggcg ggctcgccgc ctccggcgcg acgtccgggc ttgccgggct
     7201 ggccggcctg ctgctcgcga tcctgctgct agacggcgcg ctctggctcg ggctcgggcg
     7261 gctgaagctg cgtccctatc aggcgatgcg cctcacctcc ctgcacggag ccgcgacggc
     7321 cgccctctgg ctcgccgcgg ctctgctcgt cgcgcccgaa gcccagaccc ttctcgtcaa
     7381 ggcggcgctc gtcgccggcg ccggctcggc catcgccgtc ttcttcaccg tgccgctgct
     7441 gatgatcctc gccgtctgca cgaccttcct ggtcgccacg tccatgccga gcaaagagcc
     7501 ggttctcgcc atcggcggcg ccttcggcct cttcctcctg tgcctgggcg ttgcccgctc
     7561 ccgccagttc ctcttcgccg cgcgccagcg cctggcgctc gaatggcagg cggaaaaggc
     7621 acgccggttc gtcgcggatt tcgaggcgag cggccgcggc tggttttggg aaaccaacgc
     7681 cgacggcgcc ctgacctatg tctcggagcc gctcgccgcc catctcggcc ttgccgcgcc
     7741 cgagctgatc ggccggcgct tcgaggacgt cctgctgctc gattccggcg acggcgacga
     7801 cagccggcga agcctgggct tccacctttc cgcgcgcttc cccttcgccg acgccgtggt
     7861 gcgggcgccg accggcgacg acgtctggtg gtcgctgtcc ggaagcccga atttcgacgc
     7921 ttatggccgc ttcctcggct tccgcggcct cggcagcaat ttgagcgagc agcgccggag
     7981 cgaggcggag gcgagcaagc tggcgcgcta cgacagcctt accaacctgc ccaaccgggc
     8041 gatgatgcgc tcgatgctcg accaggcgct ggccaatgcc gatgcgcgcc ggcgcggctg
     8101 cgcgttgatg gtcatcgacc tcgatcgctt caagcaggtc aacgacacgc tcggccaccc
     8161 ggtcggcgac aaattgctga agaaggtcgc ggcgcgactc accgaagtgc tcggcgagga
     8221 cgggcaggtc ggccggctcg gcggcgacga gttcgaggcg atcctcccgg gaatcgagga
     8281 agagggccgg ctttccgagc tggcgaagcg gctgatcgag caggtctccc ggccttacaa
     8341 gatcgagggc cacgaggtcc ggatcggcac ctcggtcgga atcgccgtcg cggtgccggg
     8401 caagactctc gccgaagggc tgatcaagga cgcggacgtc gccctctatg ccgccaaggc
     8461 ggacgggcgc gggaccttcc gtttcttcgc ccaggagatg cacgcgctcg ccaccgagcg
     8521 gcagatactc gagaaggacc tgcgcgaggc actggccggc gaccagctga cgctcgtcta
     8581 ccagccgatc gtggactcgg tttcggaaga ggtggtcgcg ttcgaggccc tgctgcgctg
     8641 gcaccatccg gcgcgcgggc tgcttccgcc cgccctggtc atcccgctcg cggaggaaac
     8701 ggggctgatg ccgcggatcg gcgactgggt gctcaggacc gcctgtgccc aggcggcgaa
     8761 atggccgcgc catatccggg tggcggtcaa cctctccccc gtgcagttcg ccgatccggc
     8821 cctcgcggcc accgtgaccg gcacgctcgc ggcctcgcag ctcgatcccg agcggctcga
     8881 gctcgagatc aacgaggccg tgttcctcgc cgaccgcaag gtcaccgaag cgaccctcac
     8941 ccgcctgcgc ggcctcggag tccgactcgc tctcgacaat ttcgggaccg gccattcggg
     9001 cctcggccat ttgcgcgacg cgccgctcga caagatcaag atcgaccaga gcttcgttcg
     9061 cggagcgacc gcgccgggca gccgcaacgg cgccatcgtc cgggccatcg tcgtcctcgc
     9121 cgagagcctg ggcatggaca cgacggcgga aggggccgag acgatcgagg agctgtcgct
     9181 gatccgccgc ctcggctgct cgcaggtgca gggcttcatc ttcgccaggc cgatgccggc
     9241 tgaggaagcg ctggcgctcg ccacggagag ccggccatcg gccgaggtgg tcggcttcag
     9301 ccgcccgccc cgccaccggc tgatccgcaa cggccgactg ctctggcagg gccggaccct
     9361 gccggtgcgg ctgcgcaata tctccaccgg cggggcgatg atcgagtgcg acagcgagct
     9421 cgagccgggc accccgatcg agctcgatct ggccgaggcc gggactctcc cggcggaagt
     9481 gcgctggtgc cagcagggcc aggtcgggct tcacttcggc gaggaattcg ccctcggcaa
     9541 gctcgcccgc acccggcgca agggcgccgc tccgcaggcg ctgaagccgg aatatccgga
     9601 gccccgcaag gcggcgaagt agaccgtcat cccggcgaaa gccgggacct caggccgaaa
     9661 gggcgactgt gcgggcctcg ccgaggtccc ggctttcgcc gggatgacga aggtggcccg
     9721 tcagctcgaa taataaaggt cgtactcgac cgggctgggt gtcgtctccc agcgcatctg
     9781 ctcctcccac ttgagctcca tgtaggcgtc gatctggtcg tcgctgaaga cgtcgccctt
     9841 ggtcaggaag gcgcggtcgt tctggaggga gatgagcgcc tcgcgcaagc tgccgcagac
     9901 ggtggggacg ttgaccagct cctgcggcgg caggtcgtag agattcttgt ccatcgggtc
     9961 cccgggatgg atccggttct ggatcccgtc gaggcccgcc atcaggatcg ccgaataagc
    10021 gaggtacgga ttggccagcg cgtcggggaa gcggaactcg acgcgcttgg ctttctcgcc
    10081 ggcgccatag gggattcggc aggaggcgga gcggttgcgg ctcgaataag cgagcagcac
    10141 cggcgcctcg aagccgggga cgagccgctt gtagctgttc gtcgtcgggt tggtgaaggc
    10201 gttcagcgcc ttggcgtgcc tgatgactcc accgatgaag tagagcgcca tctccgaaag
    10261 gccggcatag ccgttgccgg cgaacagggg cttgccgccg ttccagatcg agaagtgggt
    10321 gtgcattccc gagccgttgt cgctcttgat cggcttgggc atgaaggtcg cggtcttgcc
    10381 ataggcctgg gcgaccatgt ggaccacgta cttgtagatc tgcatccggt ccgcggtctg
    10441 gacgagggtg ccgtaggtga ggcccaattc gtgctgggcg gcggcgacct cgtggtggtg
    10501 cttgtccatc ggaaggccca tttcgagcat ggtcgaaacc atctcgcccc ggatgtcggt
    10561 ggcgctgtcg accgggccga ccgggaaata gccgcccttg gcgcggggcc ggtgggcgag
    10621 attgccgcct tcatattcgc ggccgctgtt ggtcggaagc tcgatgtcgt cgatccggaa
    10681 gtaggaggcg ttgtagccgt cctcgaagcg gacgtcgtcg aacatgaaga attcggcttc
    10741 ggggcccacg aagacggtgt cgccgacgcc ggtgcccttg aggtagttct cggcgcgctt
    10801 ggcggtcgag cggggatcgc gggcgtagag ctcgccggtg cccggatcga cgatgtcgca
    10861 gaagacgacc agcatcgggg tggcgctgaa cgggtcgaca tagacggcgt cgaggtcggg
    10921 cttgaggatc atgtccgact cgttgatcgc cttccagccg ttgatcgagc tcccgtcgaa
    10981 catgaagccg tccgacagct ggtcctcgtc gaccgctccg gcgaccatcg agagatgctg
    11041 ccacttgccg cgcgggtcgg tgaacctcag gtccacccac tcgatctcct cgtcctcgat
    11101 ccgcttcaga accttgcccg cgtcgctcgc catgatgccc tcgcttttcg tccggtgaag
    11161 tgcgcccgcc gactcgggcg attgctcgga ccaagccttt ccccgcggtc cggcgagggt
    11221 ccagccgggg gtgaacaatt cacgtggaaa gctagaagtg ccttctctga gccgcgatca
    11281 gcatcctcag cagcatcgcc accggcctcg gcactccgac cttgccctcg agccagaggc
    11341 tgaccgccga acggctgacc ccgatcgcgg cggcgagatc gttctgggtg cgatagccca
    11401 gggtgcgcat cgcggcgcgc aactcctcgg gcgacatgga ggcgaggcgg gggtcgtcca
    11461 cgcgcccgcc ttagcgcgcc ggccgggcgg gaggaagttt taggcggccg cggggacaga
    11521 cgtttattgt cgtgaaaccg gatcgcctta tggtggagcc atgctgcttc tcgccgccgc
    11581 cctcgccgcc caggcgcccg gaccgtcctt tccctgcgag cgcgcatcgg cgccccagga
    11641 gaagctggtc tgctcgcgcc gaacgctcgc caaggcggat ctggagatgg actccgccta
    11701 tcgcggggct ctcgccaagc tgaaccccgc cgggcgccgg caattgcggc tgagccagaa
    11761 cgcctggctg agattacgta acgcggtctg cccgctcggg cgccgtgacc cgccgaatcg
    11821 gcaatcgacg gaaacctgcc tccaggcgcg ctacgaggag cggacccacc agttgaacga
    11881 ggccgtccgc cggttcggcc cgttccgggt cgttcgactc gacggctacg aggtctggcc
    11941 ggcgcccgaa cgcgacgcct ggtacgacac cgtcgtgacc aacgaggaaa ggttcgactg
    12001 gatcgacgtg gacgccgccc cggcgccgct tcgaccggcg gcgcggacct ggaatgcggg
    12061 gctggacagg cggcccacga cggccggcgc gcgatggcgt ctgcacggcc ggggacgagt
    12121 tcagacggat gacggatcca acgatggccc ggacgtggac acggggcgga atctggagat
    12181 aatcgccgcc tcgcccgacg cgatcgtcag ccgtgaagag gattattgga tgggctcgac
    12241 ccatcccgag gaaaatatca gctacgagac cctgctgatc cgcgagcggc gggcgatgcg
    12301 cttcagcgac ctcctcaccc ggccggcgga gtgggggcgc ttcgcgaaaa agcgggtcat
    12361 gtccatcccg ccgatgaaca ccgtcaagga ctggccgagg ctcgccgaaa ccgcgaccga
    12421 tcctggatcg ctgctccccg gccccgccgg gctcactgtc aatttcggga ggccgctgg
//
LOCUS       DASWNU010000154         4981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_57382, whole genome shotgun sequence.
ACCESSION   DASWNU010000154 DASWNU010000000
VERSION     DASWNU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57382"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VGB57_03000"
     CDS             <1..567
                     /locus_tag="VGB57_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130346.1"
                     /translation="VSEAVSILDSRTISTRIVQPDLDRTTRMRVARDGRTATYVSIDH
                     RRGIPGKLRTEYLASRAAPGPADSHAVSGTWRGVRYVSVPVQLRTTTLSDDGGRLSYR
                     TGAGFSYSAPYGGAFVPIEGPYDGSLSVSVRKDGPYRMVETRRRGGADVQVRTYTVSS
                     DGRRMETSTTDLATNATFRITARRQRKR"
     gene            648..1673
                     /locus_tag="VGB57_03005"
     CDS             648..1673
                     /locus_tag="VGB57_03005"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY0130347.1"
                     /translation="MALYPLVRPLAFALDAERAHRLTIRALKLVPAGEPPREDSRLAI
                     RVAGLDFPNPVGLAAGFDKDAEVHRQMLGFGFGFVEIGTLTPRPQSGNPRPRLFRLAE
                     DRAVINRMGFNNGGFEAARTRLAGRRRGILGVNIGANKDSADRIADYAAGAREMAPAA
                     DYLTVNISSPNTPGLRALQDKGALAELLAAVGEGRSGSTPIFLKVAPDLAADEIDDIA
                     RAAMDNRVDALIVANTTVSRPDLRSKLRGQAGGLSGAPLRALALQRLKDFRAATGGAM
                     PLIAVGGIENGVDAYDRIRAGASLVQLYTALVYQGPGVARSIAGELRYLLARDGFATL
                     ADAVGAS"
     gene            1713..3413
                     /gene="ggt"
                     /locus_tag="VGB57_03010"
     CDS             1713..3413
                     /gene="ggt"
                     /locus_tag="VGB57_03010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0130348.1"
                     /translation="MLSRLFLPLLLLLASCATASDPAPAGGTEAGGMVSAADPRAAEA
                     GRWILNQGGNAADAAIAIQIALTVVEPQSSGIGGGGFFVYHDAAKGRVFTYDGREKAP
                     DAASPTWFFTPDGKPLPYRQAIPGGKSVGVPGNIRLMALAHSRHGRLPWAKLFEPAIR
                     LARDGFALTPRMRNAIGNNRDLIGLSAWGRGYLFGADGEPKAAGTVLRNPELAQLLEN
                     VAARGPDYFYVGPAAEALVRTVRGAPRNPSAMVVGDLAAYEAKERPPVCAPYRGYRIC
                     GMGPPSSGATTVYATLKQIERFDLRALGKDNPVAWHLIGNSMRLAFADRDAYLADPDF
                     VRVPVAGLMNPDYLASRSRLISADRAIDKAVAGTPPGAPRVTAAAAFEVPSTSHMAAA
                     DGAGNVASVTSTIEGAWGSGLTVNGMFLNNELTDFSIAPEVDGVPVANRVEGGKRPRS
                     SMAPTIVYGPDGKVRLAVGAAGGPTIIAQVAKAIIAVIDWDLSAQEAIALPTIFAPGD
                     TLMVERGTSLEAMIPALRALGNKAAPMNPGFKANAIEWVDGGWRGGADPRSEGVALAQ
                     "
     gene            3443..>4981
                     /locus_tag="VGB57_03015"
     CDS             3443..>4981
                     /locus_tag="VGB57_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0130349.1"
                     /translation="MRELEHFPNLVAMFFARAAQKGDSPFLWAKKQGEWRSISWREAA
                     DQVCSLAAGLKGIGLRPGDMVMLVSENRPEWCIADLAIMTAGCVTVPAYTTNTERDHQ
                     HILDDSGARAVIVSTQKLARPLLPAAIRSSNCEHVIGIEALPGGQSGNVGFHDWKTLI
                     AAHAPDVAACTETAGRVTRDDLACLIYTSGTGGAPRGVMQHHGAILHNVEGCIEVISE
                     DFGVDEEVFLSFLPPSHAYEHSGGQFFPIGIGAQIYYSEGLEKLASNIEEARPTLMIV
                     VPRLFEVLRQRILKNVEKQGKLANYLMKRALEIGARDYAGARSLWDRPMDLVLDRLFR
                     PKISARFGGRVKAMVSGGAPLNPDVGIFFHSIGLTVLQGYGQTETGPVISCNRPKAGV
                     KMDTVGPPLRNTEVRIAEDGEILVRGELVMKGYWRNQGLTEKTIVGGWLHTGDVGHID
                     EAGRIVITDRKKDLIVNDKGDTVAPQKVEGMLTLQPEIVQAMVVGDKRPYVVGLIVPD
                     PEWSA"
BASE COUNT          799 a         1724 c         1704 g          754 t
ORIGIN      
        1 gtctcggagg ccgtttcgat cctcgattcg aggacgatct cgacccgcat cgtccagccg
       61 gacctcgatc gcacgacgag gatgcgggtg gcccgcgacg ggcgcacggc cacctacgtc
      121 tcgatcgacc atcggcgagg aatcccggga aagctccgca ccgaatatct ggcgagccga
      181 gccgcgcccg ggccggccga tagccatgcc gtgtcaggca cctggcgggg cgtgcgctac
      241 gtctcggtcc cggttcagct ccggacgacc acattgagcg acgacggcgg ccggctgagc
      301 taccgcacgg gcgccggctt ctcgtacagc gcgccttacg gaggcgcgtt cgtgccgatc
      361 gagggcccct atgacggaag cctctcggtc tcggtccgaa aggacggtcc ctatcggatg
      421 gtggagaccc ggcggcgagg cggcgccgac gtgcaggtgc gcacctatac cgtctcgagc
      481 gacggccggc ggatggagac gtcgaccacc gatctggcga cgaacgccac cttccggatc
      541 accgcccgaa ggcaaaggaa gcggtagcgg gccgcgaccc ttcacccccc ggttcgtttc
      601 agagccagat ggtcactcgc cgaggcctat gccatagccc ccccgcaatg gcgctctacc
      661 ccctggtccg gcccctcgcc ttcgcgctcg acgcggagcg ggcgcatcgg ctcaccatac
      721 gggcgttgaa gctcgtgccg gcgggcgagc cgccccggga ggacagccgg ctcgccattc
      781 gtgttgcggg gctcgatttc cccaatccgg tcggtctcgc cgccggcttc gacaaggatg
      841 ccgaggtcca ccggcagatg ctcggcttcg gcttcggctt cgtcgagatc ggcaccctga
      901 ccccgcgacc gcagtcgggt aatccgcggc cgcgcctgtt ccggctcgcc gaggaccggg
      961 cggtgatcaa ccggatgggc ttcaacaatg gcggcttcga agctgccagg acccggctgg
     1021 cggggcggag gcgggggatt ctcggggtca atatcggggc gaacaaggac agcgccgacc
     1081 ggatcgcgga ttacgccgcg ggcgctcgcg agatggcccc ggcggccgac tacctcaccg
     1141 tcaatatttc gtcgcccaac actcccgggc tgcgcgcgct gcaggacaaa ggcgctctgg
     1201 cggagctgct tgccgcagtc ggcgaggggc ggagcggctc gactccgatc ttcctcaagg
     1261 tcgctccgga cctcgcggcg gacgaaatcg acgacattgc ccgggccgcg atggataacc
     1321 gggtggatgc gttgatcgtc gccaacacga cggtctcccg gccggatttg cgctcgaagc
     1381 tccgcggcca ggcgggcggc ctttcgggcg cgccgctaag ggcgctcgcc ctgcagcggc
     1441 tgaaggattt ccgagccgcg accggcgggg cgatgccgct gatcgccgtc ggcgggatcg
     1501 agaacggagt cgacgcctat gaccggatcc gggccggggc gagcctcgtc cagctctaca
     1561 cggcgctcgt ctaccaggga ccgggcgtcg cccggtcgat cgcaggcgag ctgagatatc
     1621 tgctcgccag ggacggcttc gcgaccctcg ccgacgcggt cggggcgtct tagcgcttgc
     1681 ggcgcgggcg aaggcggcta gtgtcgccgc cgatgctcag ccggcttttc cttccgctcc
     1741 tcctcctcct cgcgtcctgc gcaaccgcct ccgatccggc cccggccgga gggacggaag
     1801 cgggcgggat ggtctcggcc gccgatcccc gggcggccga agcggggcgc tggatcctga
     1861 accagggcgg caacgccgcc gacgccgcca tcgccatcca gatcgctttg accgtggtcg
     1921 agccgcaatc gtcgggcatc ggcggcggcg gcttcttcgt ctatcacgat gccgcgaaag
     1981 ggcgggtctt cacctatgac ggccgcgaga aggctccgga cgccgcctcg ccgacctggt
     2041 tcttcacgcc ggacgggaag ccgctgcctt atcgccaggc cattcccggc ggcaagagcg
     2101 tcggagtgcc cggcaacatc cggcttatgg cgctcgccca ctcccgccac ggccggctgc
     2161 cctgggcgaa gctgttcgag cccgcgatcc gactcgcccg cgacggcttc gctctgactc
     2221 ccaggatgcg caatgcgatc ggaaacaacc gggacctgat cggcctgagc gcctgggggc
     2281 gcggctatct cttcggggcg gacggggagc cgaaggcggc cgggacggtc cttcgcaatc
     2341 ccgagctggc gcagctgctc gaaaatgtgg ccgccagggg acccgattat ttctacgtcg
     2401 gcccggcggc ggaggcgctc gttcgaaccg tccgaggcgc gccgcgcaac ccctccgcca
     2461 tggttgtcgg cgatctcgcc gcctacgagg cgaaggagcg gccgccggtc tgcgcgccct
     2521 accgcggcta ccgcatctgc gggatgggcc caccttcgtc gggagcgacg acggtctacg
     2581 cgaccctgaa gcagatcgag cgcttcgatc ttcgagcgct cggcaaggac aatcccgtcg
     2641 cctggcacct gatcggcaat tcgatgcgcc tcgccttcgc cgatcgcgac gcctatctcg
     2701 ccgatccgga tttcgtccgc gttcccgtcg ccggactgat gaatccggac tatctcgctt
     2761 cccggtcgcg gctcatctcg gccgaccgcg ccatcgacaa ggccgtcgcg gggaccccgc
     2821 ccggagcgcc ccgggtaacg gcggcggccg ctttcgaggt cccgtccacc tcccacatgg
     2881 ccgccgccga cggcgccggc aacgtcgcgt ccgtgacatc cacgatcgaa ggggcgtggg
     2941 gctcgggcct gaccgtgaac ggcatgttcc tcaacaacga gctcacggat ttcagcatcg
     3001 ctccggaagt cgacggcgtt cccgtcgcca atcgcgtgga gggcggcaag aggccgcgaa
     3061 gctcgatggc gccgaccatc gtctacgggc ccgacggcaa ggtccggctc gccgtcggag
     3121 cggcgggcgg gccgacgatc atcgcccagg tcgccaaggc gatcatcgcg gtgatcgact
     3181 gggatttgag cgcccaggaa gcgatcgccc tgccgacgat cttcgccccc ggcgacacct
     3241 tgatggtcga gcgcggcaca agcctcgagg cgatgatccc ggcgctccgg gcgctcggca
     3301 acaaggccgc gccgatgaac cccggcttca aggccaatgc gatcgaatgg gtggacggcg
     3361 gatggcgcgg gggcgccgat ccgcgaagcg agggcgtggc gctcgcccag tgatctcgga
     3421 aaaagtgaaa tcgggagtgg ccatgcgcga gctcgaacat tttcccaatc tcgtcgccat
     3481 gttcttcgcc cgcgccgccc agaagggcga ttcgcccttc ctgtgggcca agaaacaggg
     3541 ggagtggcga tcgatcagct ggcgcgaggc ggccgaccag gtctgctctc tcgccgccgg
     3601 cctgaagggg atcggcctca ggcccggcga catggtcatg ctggtctcgg agaaccggcc
     3661 ggaatggtgc atcgccgatc tcgccatcat gaccgcgggc tgcgtcaccg tccccgccta
     3721 cacgaccaat accgagcgcg atcaccagca tatcctcgac gacagcggcg cccgcgccgt
     3781 catcgtctcg acccagaagc tcgcccggcc cctgctgccg gcggcgatcc ggtcgagcaa
     3841 ttgcgagcat gtcatcggca tcgaggcgct tccgggcggc cagagcggca atgtcggctt
     3901 ccacgactgg aagacgctga tcgcggcgca cgcgcccgac gtcgccgcct gcaccgagac
     3961 cgctggcagg gtcacccgcg acgacctcgc ctgcctgatc tacacgtcgg ggaccggcgg
     4021 cgccccgcgc ggcgtgatgc agcatcacgg cgcgatcctc cacaatgtcg aaggctgcat
     4081 cgaagtcatc tcggaggact tcggggtgga cgaggaggtg ttcctctcct tccttccacc
     4141 cagccacgcc tacgagcata gcggcggcca gttcttcccg atcgggatcg gcgcccagat
     4201 ctattattcc gaagggctgg agaagctcgc ctcgaacatc gaggaggcgc ggccgaccct
     4261 gatgatcgtg gtgccgaggc ttttcgaggt gctgcgccag cgcatcctca agaatgtcga
     4321 gaagcagggc aagctcgcca actacctgat gaaaagggcg ctggagatcg gcgccaggga
     4381 ttatgccggc gccaggagcc tgtgggaccg gccgatggat ctcgtcctcg accggctgtt
     4441 ccggcccaag atcagcgccc gcttcggcgg ccgggtgaag gcgatggtct cgggcggggc
     4501 gccgctcaac ccggacgtcg gcatcttctt ccactcgatc ggactcaccg tcctccaggg
     4561 ctacggccag accgagaccg ggccggtgat cagctgcaac cggccgaagg cgggcgtgaa
     4621 gatggacacg gtcgggccgc cgctccgcaa caccgaggtc aggatcgccg aggacggcga
     4681 gatactggtg cgcggcgagc tcgtcatgaa gggctattgg cgcaaccagg ggctgaccga
     4741 gaagacgatc gtcggaggct ggctgcacac cggcgacgtc ggccatatcg acgaggcggg
     4801 gcggatcgtc atcaccgacc gcaagaagga tttgatcgtc aacgacaagg gcgacactgt
     4861 cgccccccag aaggtggagg ggatgctgac cctccagccc gagatcgtcc aggcgatggt
     4921 ggtgggcgac aaaaggcctt acgtcgtcgg cctgatcgtg ccggacccgg agtggagcgc
     4981 g
//
LOCUS       DASWNU010000155         8632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_31883, whole genome shotgun sequence.
ACCESSION   DASWNU010000155 DASWNU010000000
VERSION     DASWNU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31883"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /gene="glnD"
                     /locus_tag="VGB57_03020"
     CDS             <1..1731
                     /gene="glnD"
                     /locus_tag="VGB57_03020"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY0130350.1"
                     /translation="ARAGRRFGLPAIRRRPRKLEGFVLDRGRLALPGDGFFAEDPVRL
                     LQIFALADLHGLEVHPLAMRAAARDSKLVDEVRDDPRANALFLEILTSPRDPETVLRW
                     MNEAGVFGRFVPDFGRVVAQMQFDMYHHYTVDEHSIRAIGLLSRIEKGELKEDHPLAT
                     VLFRHIASRRVLYAAVLLHDIAKGRGGDHSLLGAEVARALCPRLGLDEAETETVAWLV
                     RWHLLMSATAFKRDLADPQTIQDFAARVQSPERLRLLFILTVVDIQAVGPGIWNGWKR
                     QLLRELFDSAEEALRLGHKQKGRGERIAATQAALAARLGWDDPHFGRHVWRLPDSYWL
                     AEPFDVLEKNARLIDSADRAPDRTRPLAVSVEEERSATLLSVYAKDRPGLFYHLSAAI
                     SLAGANIIDARIHTTLDGMAVDNFLVQDAQGKPFADRHQLKRLEENVLRAVEGQEPAM
                     DRLEAKALPLLRAEAFAIRPAVFVDNQASSRYTVVEINARDRAALLSGLARALYESKA
                     AIHSAHIATYGERAVDVFYLTDLHGAKIEAPARLKALQSNLLRAASENGAGNGFSRPS
                     AADKRAITRS"
     gene            1866..2258
                     /locus_tag="VGB57_03025"
     CDS             1866..2258
                     /locus_tag="VGB57_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130351.1"
                     /translation="MPNTFIGTSGDDVIYGSNGSDIYYPGQNQYGDTIYDMDGDDQYI
                     LQYGYAEIFDWGGTDVVTMVGVNLADLNFFTDTWTVGILSDDQSRGVWFSGMWDNPAG
                     GVETLILDDVTLSRAEILAYAGDNIITF"
     gene            complement(2422..3684)
                     /locus_tag="VGB57_03030"
     CDS             complement(2422..3684)
                     /locus_tag="VGB57_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0130352.1"
                     /translation="MKTGATSGGRVLAILLIAYIFNFIDRQIIGVLAVPIKAELGLSD
                     GQLGLMGGIAFALFYSGLAIPIAWLADRKSRVNIIATSVALWSAFTAVCGLAQNFWHL
                     FLARMGVGIGEAGGVAPSYALISDFYPKERRARALALFSLGIPIGSALGVFFGGWIAS
                     HLDWRSAFLIVGLAGLPAALLVKLGVPEPVRGGFDTADGQASEPAPPFDRVVATLAGK
                     PSFWLLSFGAASGSILGYGLIFWLPSFFSRSFGLELAEVGWFYGSIVLIGGVAGTLLG
                     GWIGDRVGAANPGAYALVPAICFLIAAPIFAFGLFAPSLTVGWILFTIGQMLALAWLG
                     PVIAAVQHIVPPNMRATASASFLFINNLIGIGFGIFFLGFMSDRMGTAHGDDALQYSI
                     LYGLGFYVLSALIYLAASTRLKRDWYRG"
     gene            complement(3860..6259)
                     /locus_tag="VGB57_03035"
     CDS             complement(3860..6259)
                     /locus_tag="VGB57_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0130353.1"
                     /translation="MTRSRTLTALRLGAAVAALAAVPAPLYAQTEEEAAAAANQALDE
                     SASAGPVEDSEIIVTARRRAENLQDVPIAVTAYSGEALERAGAIDITDISDTTPNVTI
                     ETSRGTNTTLTAFIRGVGQQDPVAGFEAGVGLYLDDVFLNRPQAAVLDIYDVERIEVL
                     RGPQGTLYGRNTIGGAIKYVTRRLSELPTFRARANLGTYRQADLILSASTPVADGIKI
                     GGSVARLSRQGFGDNLTTGQENYNRDVWAARGTVELDPTDTISIRFSGDYTKDDSNPR
                     GGHRLIPGLLSGAPVLGDVYDSRGGLVTPKQEVKAYGVASHIDIELTEAVTLRNITAY
                     RRDRSATPIDFEALPARDVDVPAIYRNRQFSNEFQVLVDSGPLNAVLGVYYLNANANN
                     VFDVVLNEQGIRLAVPAPGFSVSTTGDVDTQTWAAFADLTYDVSDLVHLSAGGRYTVD
                     RRRAVITRRNLVFGPLPELGGQGVGGAFTSNFTGEETFKKFTPRASISFTPNDDHNIY
                     ASYSQGFKGGGFDPRGISTQAPDLNGNGVREPNEIFDYFLFDPETVNSYEIGYKASLF
                     DRRVRLALAGFYADYKDVQVPGSAGAVINGIPTFVGITTNAGKASFKGVEAEVFATLM
                     RNPNGSRLNFAGTLGYLDAQYDEFITNVPGTGPVDVANFRRIQNTPKWTLSGTLDYSM
                     RMAGGDLSASTTLSYRSKTFQFETPSPYLDQPGYALLDASLVWTSSDDRWTVGLHGKN
                     LTDKQYITSGYQFLVSNPTTGQPIINAAGNPTPSLGLEGVATTFYGNPRQVFLSLGVK
                     F"
     gene            complement(6279..7100)
                     /locus_tag="VGB57_03040"
     CDS             complement(6279..7100)
                     /locus_tag="VGB57_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0130354.1"
                     /translation="MRHFTASDGARIAYSDEGQGRPIVLLHGLMANAGFFGSQRELAD
                     SFRVVTVDLRGHGRSPAGPVPPTIRLLADDVAALARALGLEGAVAVGWSLGAAVLWRV
                     LAGPASKMFAGAVVVDMTPRVLNDRDWGLGLSRETCEARTQAIRDDFPAFAAAAGQAI
                     FASPGPQADRAAQAFAANDPAPIGALWESLVEDDFRPLLPSIRQPTLVVHGARSHLYG
                     ADTAGHLVAALPNARSVTFDRSGHSPHLEQPELFNATLRDFAATLPSVRQAQATV"
     gene            7334..8263
                     /locus_tag="VGB57_03045"
     CDS             7334..8263
                     /locus_tag="VGB57_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0130355.1"
                     /translation="MTAAAISIVELEKTYAGGKRALDAVSLDVPQGEIFGLLGPNGAG
                     KSTLINILAGLVNKSGGSVTIWGFDVDKHPRNAKASIGIVPQELVFDPFFTPRETLEI
                     TAGLYGVPKERRRTDDLLRAVRLEDKADAYSRTLSGGMKRRLLVAKAMVHSPPILVLD
                     EPTAGVDIELRKQLWAYVRQLNAEGVTIVLTTHYLEEAENLCDRIAIINHGRLIANET
                     TRTLVGMAQEKAVEVTVDRDIAALPDATCFEKIDLMGERTLAITYRKDKVNAGEVLAA
                     LQRDGLGIVDVSTREADLEDVFLNLTRASDAAG"
     gene            8260..>8632
                     /locus_tag="VGB57_03050"
     CDS             8260..>8632
                     /locus_tag="VGB57_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0130356.1"
                     /translation="MTHEFDVVVIGSGAAGLSAALTLAPRLKVAVLAKGGLSSGSTAL
                     AQGGIAAVLEAGDTFESHIEDTIVAGAGLNRRETVEFVVESAPAAIERLAALGVPFDS
                     GGGERWHLTREGGHSHRRIVHV"
BASE COUNT         1480 a         2842 c         2923 g         1387 t
ORIGIN      
        1 gcccgcgccg gccgccgctt cggcctcccc gcgattcgtc gccgtccccg caagcttgaa
       61 ggcttcgtcc tcgatcgcgg ccggctcgcg ctcccgggcg acggcttttt cgctgaagac
      121 ccggtccgcc tccttcagat cttcgccctc gccgaccttc acggcctcga agtccatccg
      181 ctggcgatgc gcgccgccgc ccgcgactcc aagctggtcg acgaggtgcg ggacgatccg
      241 cgcgccaacg ccctcttcct cgaaattctg accagccctc gcgaccccga gacggtgctg
      301 aggtggatga acgaggcggg cgtgttcggc cgcttcgttc ccgatttcgg ccgggtcgtc
      361 gcccagatgc agttcgacat gtatcaccat tatacggtcg acgagcacag catccgggcg
      421 atcggcctgc tctcgcggat cgagaagggc gagctgaagg aggatcatcc gctcgccacc
      481 gtccttttcc ggcacatcgc ctcgcgccgg gtcctctacg cggccgtcct cctccacgac
      541 atcgccaagg gccgcggcgg cgatcacagc ctgctcggcg cggaggtggc tcgagcgctc
      601 tgcccaaggc tcggcctcga cgaggcggag accgaaacgg tggcatggct ggtgcgctgg
      661 cacctgctca tgtcggccac ggccttcaag cgcgacctcg cggacccgca gaccatccag
      721 gacttcgccg ccagggttca gagcccggag cggcttcgac tgctcttcat cctcaccgtc
      781 gtcgacatcc aggcggtggg gcccgggatc tggaacggct ggaagcgcca gctgcttcgc
      841 gaattgttcg actccgccga ggaggcgctc cgcctcggcc acaagcagaa gggccggggc
      901 gagcggatcg ccgccaccca ggccgcgctg gcggccaggc tcggctggga cgacccgcat
      961 ttcgggcggc atgtctggcg ccttcccgac agctactggc tcgccgagcc tttcgacgtc
     1021 ctcgagaaga atgcgcggct gatcgactcg gccgaccgcg ctcccgaccg gacccggccg
     1081 ctcgccgtct cggtcgagga ggagcgctcc gccactttgc tcagcgtcta cgccaaggac
     1141 cggcccggcc tcttctacca cctgagcgcc gcgatcagcc tcgccggagc caacatcatc
     1201 gacgcccgga tccatacgac tctcgacggc atggcggtcg acaatttcct cgtccaggac
     1261 gcccagggga agccgttcgc cgaccgccat caattgaagc gtctcgagga gaatgtgctt
     1321 cgggcggtcg aggggcagga gcctgccatg gaccggctcg aggcgaaggc gcttcccctg
     1381 cttcgcgccg aggccttcgc catccggccc gccgtcttcg tcgataacca ggcgtccagc
     1441 cgctacaccg tcgtcgaaat caacgcccgc gaccgagcgg ccctgctcag cgggctcgcc
     1501 cgggccctgt acgaatcgaa ggcggcgatc cacagcgccc acatcgccac ttacggcgag
     1561 cgggccgtcg acgtcttcta cctgacggat ctccacggcg ccaagatcga ggcccccgcc
     1621 cggttgaagg cgctccagtc gaacctcctc agggccgcct cggaaaacgg tgccgggaac
     1681 gggttttcgc gcccgtcggc cgcggacaag cgggcaatca caagaagtta gaatggtggc
     1741 tgatgccgat cgaagtcgaa acggatcatt cttcggtttc ttcggcagct cgcattgatt
     1801 aagccggtca atagtacttc aacatgattg aagtcaggcg cggctatagt atgagcgagg
     1861 tctccatgcc taataccttc atcggcacgt ccggagatga tgtcatctac ggttccaatg
     1921 gctccgatat ctactatccg gggcagaacc aatatggcga taccatctac gacatggacg
     1981 gagacgacca gtacattctc caatatggtt acgccgagat cttcgattgg ggcgggacgg
     2041 acgtcgtcac catggtcgga gtgaatctcg cggatctgaa cttcttcacg gacacatgga
     2101 cggtcggcat cttgtcggac gaccagtcga ggggcgtctg gttctccggt atgtgggaca
     2161 atccggccgg cggtgtcgaa acgctcatcc tggacgacgt gaccctcagc cgtgccgaga
     2221 tcctcgccta cgccggtgat aatatcatca ccttctagcg cggcgcacgc aaattcgcac
     2281 cgcctcgtca tgccggactt gatccggcat ccaccttcgc tttgccagct ggagcaagaa
     2341 ggtggatccc ggatcaagtc cgggatgacg attcaaacct gacgcccgac gctctagcgc
     2401 gaatcgccgc ggccggctgg gtcagccgcg gtaccagtcg cgcttcaggc gggtcgaggc
     2461 ggctaggtag atgagcgcgc tcaggacgta gaagccgagg ccgtatagga tcgaatattg
     2521 gagggcgtcg tcgccgtgcg ccgtccccat ccggtcggac atgaagccca ggaagaagat
     2581 cccgaagccg atcccgatca gattgttgat gaacaggaag ctggccgagg cggtcgcgcg
     2641 catgttcggc ggaacgatgt gctggacggc ggcgatcacc ggcccgagcc aggccagagc
     2701 cagcatctgg ccaatcgtga acaggatcca gcccaccgtc agcgacggcg cgaacaggcc
     2761 gaaggcgaag atgggcgcgg cgatcaggaa gcagatcgcg gggacgagcg cataggcgcc
     2821 gggattggcg gcgccgacgc gatcgccgat ccagccgccg agcaaggtgc cggcaactcc
     2881 gccgatcagc acgatcgacc cgtagaacca gccgacctcg gcgagctcga gcccgaagct
     2941 ccggctgaag aagctcggaa gccagaagat gaggccgtag ccgaggatcg agcccgaagc
     3001 ggctccaaag gagagcagcc agaagctggg cttgccggcc agggtcgcga cgacccgatc
     3061 gaacggcggc gccggctcgc tcgcctgccc atcggcggtg tcgaagcccc cgcgcaccgg
     3121 ctcggggact ccgagcttga ccagcagggc ggcgggcagc ccggccagcc cgacgatgag
     3181 gaaggccgag cgccagtcga gatggctcgc gatccagccg ccgaagaaga ctcccagcgc
     3241 cgagccgatc gggattccga gcgagaacaa ggcgagcgcc cgggcccgcc tttccttcgg
     3301 gtagaaatcc gagatcaggg cgtaggacgg cgcgactccg ccggcctcgc cgatcccgac
     3361 ccccatgcgc gccaggaaca ggtgccagaa gttctgggcg aggccgcaga cggcggtgaa
     3421 ggcgctccac aaggccaccg aggtcgcgat gatgttgacc cggctcttgc ggtcggcgag
     3481 ccaggcgatc gggattgcaa ggcccgaata gaaaagggcg aaggcgatcc cgcccatcag
     3541 tccgagctgc ccgtccgaaa gccccagctc cgccttgatc gggaccgcca gcacgccgat
     3601 gatctgccgg tcgatgaagt tgaagatata ggcgatgagc aggatcgcga gaacgcgtcc
     3661 gcctgaggtg gcgcctgttt tcatagtgcc cggctgtgtc gacattcgct gcctttacct
     3721 gcttcctccc ctgcattcgc aggggagggg gaccaggcga agcctggtgg aggggccttg
     3781 cctgcttcga aagagcccct ccaccgcgct tcgcgcggtc cccctccccc gaaaaatccg
     3841 ggaggggttc agaggcgtcc tagaacttca ccccgaggct gaggaagacc tgcctcggat
     3901 tgccgtagaa ggtggtcgcg accccttcga gacccaggct cggggtcgga ttgcccgcgg
     3961 cgttgatgat cggctgcccc gtcgtcggat tgctgaccag gaactggtag ccggaggtga
     4021 tatactgctt gtcggtcagg ttcttgccgt ggaggcccac cgtccagcgg tcgtccgagc
     4081 tcgtccagac gaggcttgcg tcgaggagcg cgtagcccgg ctggtcgagg taggggctcg
     4141 gagtctcgaa ctggaaggtc ttgctccggt aggagagcgt cgtgctggcg gaaaggtcgc
     4201 ccccggccat ccgcatcgaa taatcgagag ttccggagag cgtccatttc ggcgtgttct
     4261 ggatgcgccg gaagttggcg acgtcaaccg ggccggtgcc gggcacgttg gtgatgaact
     4321 cgtcatattg cgcgtcgaga tagcccaggg tcccggcgaa gttgaggcgg gagccgttgg
     4381 ggttgcgcat cagggtggcg aaaacttcgg cctcgacgcc cttgaagctg gccttgccgg
     4441 cgttggtcgt gattccgacg aaggtgggga ttccgttgat cacggcgccg gccgagccgg
     4501 gaacctggac gtccttatag tcggcgtaga agccagcgag cgcgagccgg acgcggcggt
     4561 cgaagagcga cgccttgtag ccgatctcgt agctgttcac cgtctcggga tcgaagagga
     4621 agtaatcgaa aatctcgttg ggttcgcgga cgccgttgcc gttcaggtcc ggcgcctgcg
     4681 tggaaatgcc gcgcggatcg aagccgcccc ctttgaagcc ctgcgagtag ctggcgtaga
     4741 tgttgtgatc gtcgttcggg gtgaagctga tcgacgcgcg cggagtgaac ttcttgaagg
     4801 tctcctcgcc ggtgaagttc gacgtgaagg caccgccgac gccttgcccg ccaagctccg
     4861 gcaacgggcc gaaaacaagg ttcctgcggg tgatcacggc ccggcgcctg tcgaccgtgt
     4921 aacggccgcc ggcggacagg tggaccagat cgctgacgtc gtaggtgaga tcggcgaacg
     4981 ccgcccaggt ctgggtgtcg acatcgcctg tggtcgagac gctgaagccc ggagcaggca
     5041 ccgcgagccg gatgccctgc tcgttgagca cgacgtcgaa cacattgttc gcattggcgt
     5101 tcagataata gacgcccagc accgcgttga gcggaccgct gtcgaccaga acctggaatt
     5161 cgttggagaa ttgccggttg cgatagattg ccggaacatc gacgtcgcgg gcgggcaggg
     5221 cctcgaaatc gatcggagtg gcggagcggt cgcggcgata ggcggtgatg ttgcgaaggg
     5281 tgacggcttc ggtcagctcg atgtcgatat gggacgcgac tccataggcc ttgacctcct
     5341 gcttgggcgt gacgaggccg ccgcggctgt cgtagacgtc gccgagcacc ggggctcccg
     5401 acagcagccc cgggatcagc cggtggccgc cgcgcgggtt gctgtcgtcc ttggtatagt
     5461 cgcccgagaa gcggatggag atggtgtcgg tcggatcgag ctcgacggtg ccccgcgccg
     5521 cccagacgtc gcgattgtaa ttctcctggc cggtggtgag attgtcgccg aagccctgcc
     5581 ggctcagccg ggccaccgaa ccgccgattt tgatcccgtc cgcgaccgga gtgctcgccg
     5641 agaggatgag gtccgcctgc cggtaggtgc cgagattggc gcgggcgcgg aacgtgggca
     5701 gctcggaaag acggcgggtg acgtatttga tcgctccgcc gatcgtgttg cggccgtaga
     5761 gcgtgccttg cgggccgcgc aggacctcga tccgctcgac gtcgtagatg tcgagcaccg
     5821 ccgcctgggg acggttgaga aacacgtcgt cgagatagag tccgacgccg gcctcgaagc
     5881 cggcgaccgg atcctgctgc ccgacgccgc ggatgaaggc ggtgagggtc gtgttggtgc
     5941 cgcgcgacgt ctcgatcgtg acgttggggg tggtgtccga gatatcggtg atgtcgatcg
     6001 cgccggcgcg ctccagcgcc tcgcccgaat aagcggtgac ggcgatcgga acgtcctgga
     6061 ggttctccgc gcggcggcgc gcggtgacga tgatttcgct gtcctcgacc ggtcccgcgc
     6121 tcgccgattc gtcgagcgcc tggttggcgg cggcggccgc ttcctcttcg gtctgggcgt
     6181 aaagcggggc cgggacggcg gcgagcgcgg cgacggcggc gccgagcctc agggcggtca
     6241 gggtgcggct acgggtcatg gcggtgtctc ctggcattct agacggtggc ttgggcttga
     6301 cggactgacg gcaaggtggc cgcgaaatcg cgaagggtgg cgttgaacaa ttcgggctgc
     6361 tcgaggtgcg gcgaatggcc ggagcgatcg aaggtgaccg agcgggcatt gggcagagcc
     6421 gcgacgaggt ggccggcggt gtcggcgccg tagagatggc tccgggcgcc gtggacgacg
     6481 agcgtcggct gccggatgga ggggaggagc ggtcggaaat catcctcgac cagcgattcc
     6541 cacaaggcgc cgatgggggc gggatcgttg gccgcgaagg cctgcgccgc ccggtccgcc
     6601 tgcggcccgg gcgaggcgaa gatcgcttgg ccggccgcgg ccgcgaaggc cgggaagtcg
     6661 tcccgaatcg cctgggttcg cgcttcacag gtctcgcgtg agaggccgag gccccaatcg
     6721 cggtcgttca ggacccgtgg agtcatgtcg accaccacgg cgcccgcgaa catcttcgag
     6781 gcaggcccgg cgagaacgcg ccagagcacg gcagccccca gcgaccagcc gaccgccacc
     6841 gcgccttcca ggcccagcgc ccgggcgaga gccgccacgt catcggcgag gagacggatc
     6901 gtgggcggga ccggcccggc aggggaccgg ccgtggcccc gcaggtccac cgtcaccacc
     6961 cggaaggagt cggcgagctc gcgctgcgag ccgaagaagc cggcattcgc catcagcccg
     7021 tgcagaagca cgatcggacg cccctggccc tcgtccgaat aggcaattcg agcgccgtcc
     7081 gatgccgtaa agtgcctcaa cccctctccc ccggccgcgc gcgctccttc ggtggagtct
     7141 caaggcggct ccggcctttc atgcctcaag atgaaacctg agtcaatgtt caacttaatc
     7201 ccgtcccagc tggctggaac cccggcgaag catgtgccgt gcctttcacc cgctgcggcc
     7261 ccagccttcg ccggggcgac ggtgcagccc ggcactccta tgcttccccg tctccagtcg
     7321 ctaaagcccg cgcatgacag ctgccgcgat ctccatcgtc gaactcgaaa agacctatgc
     7381 cggcgggaaa agggcgctgg acgcggtcag cctggacgtt ccgcaggggg agatattcgg
     7441 tctgctcggg cccaacggcg ccggcaaatc gactctgatc aacatcctcg ccggcctggt
     7501 caacaagagc gggggcagcg tcaccatctg gggcttcgac gtcgacaagc atccgcgcaa
     7561 cgccaaggcc tcgatcggga tcgttcccca ggagctggtc ttcgacccct tcttcacgcc
     7621 ccgcgagacg ctcgagatca cggccggcct ctacggagtc ccgaaggagc ggcggcgcac
     7681 cgacgatctg ctccgcgccg tccggctcga ggacaaggcc gacgcctatt cgcgcaccct
     7741 ctcgggcgga atgaagcgcc ggcttctggt cgccaaggcg atggtccatt cgccgcccat
     7801 cctagtgctc gacgaaccca ccgccggggt cgacatcgag cttcgcaagc aactctgggc
     7861 ctatgtccgg cagctcaacg ccgagggcgt gacgatcgtc ctcaccaccc attatctcga
     7921 ggaagcggaa aacctctgcg accggatcgc gatcatcaac cacggccggc tcatcgcgaa
     7981 cgagacgacc cggaccctgg tcggaatggc tcaggagaag gcggtcgaag tgacggtgga
     8041 ccgcgacatc gcagctttgc ccgacgcgac ctgcttcgag aagatcgacc tgatgggcga
     8101 gcgcaccctc gccatcacct accgcaagga caaggtgaat gccggcgagg tgctcgccgc
     8161 cctgcagcgc gacggcctcg ggatcgtcga cgtctccacc cgcgaggcgg atctggagga
     8221 cgtgttcctc aacctcaccc gggcctcgga cgccgcgggg tgacccacga attcgacgtc
     8281 gtcgtgatcg gctcgggcgc cgccggcttg tcggcggcgc tcaccctcgc cccgcgcctc
     8341 aaggtcgccg tgctcgccaa aggcggcctc tcctccggct cgaccgctct cgcccagggc
     8401 gggatcgcgg cggtgctcga ggccggcgat acgttcgaga gccatatcga ggacacgatc
     8461 gtcgccggcg ccggcctcaa ccggcgcgag acggtcgagt tcgtcgtcga gagcgccccc
     8521 gccgccatcg agcggctcgc cgccctgggc gtccccttcg actcgggcgg cggggagcgc
     8581 tggcacctga cccgcgaggg cggccacagc caccgccgca tcgtccacgt cg
//
LOCUS       DASWNU010000156         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_188059, whole genome shotgun sequence.
ACCESSION   DASWNU010000156 DASWNU010000000
VERSION     DASWNU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188059"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..857
                     /locus_tag="VGB57_03055"
     CDS             63..857
                     /locus_tag="VGB57_03055"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY0130357.1"
                     /translation="MQPYLHLLSRILDEGAEQQDRTGVGTLSVFGHQMRFDLAEGFPL
                     LTTKKLHLRSIVIELLWFLRGETNIAWLKENRVRIWDEWADEGGELGPVYGKQWRDWE
                     GPGGVHVDQIANLIELINRHPASRRQIVSAWNPAEIERMALAPCHCLFQTQVARGRLN
                     LQLYQRSADAFLGVPFNIASYALLTHMLAQQCGLEPGVFVWTGGDCHLYSNHLEQARL
                     QLTREPRPLPRLNILRRPEAIDRYEYEDFEITGYDAHPHIKAEVAV"
     gene            957..1424
                     /locus_tag="VGB57_03060"
     CDS             957..1424
                     /locus_tag="VGB57_03060"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY0130358.1"
                     /translation="MPTITVVVARARNGAIGRHGTLPWRLPADLKHFKAVTMGSAMVM
                     GRKTFDSLPGLLPGRRHIVLTRDPRWRAEGAETARSVDEVIGLAADEPVSVIGGAEII
                     ALFLPRADRVELTEVHDEPEADTFMPAFDPVEWREVSRADHPGYSFVRLERRT"
     gene            1563..>2151
                     /locus_tag="VGB57_03065"
     CDS             1563..>2151
                     /locus_tag="VGB57_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M19"
                     /protein_id="HEY0130359.1"
                     /translation="MRKFIYGLLALIVVAVGAFLILGPGIVESGMNKVVATPLPTISE
                     RARALHPRLAVADMHADTLLWQRSLLDPASRGQVDLPRLEAGNVALQIFSSVTKTPKG
                     QNYDANGADTDNITLLAVAQLQPPRTWNSLLQRSLWHSEKLRRAEAGSEGRLRIVRSG
                     IDVRRLLADRAAGRKVVGALLSIEGLQNLEGRLDNL"
BASE COUNT          370 a          745 c          699 g          337 t
ORIGIN      
        1 ggctttcgcc gggatgacgg actgagcttg gaaacgcccg ccccaaccgc catagtcccg
       61 ccatgcagcc ctatctccac ctcctttccc ggatcctcga cgagggcgcc gagcagcagg
      121 accgcaccgg cgtcggcacc ctctccgtct tcggccacca gatgcgcttc gacctggccg
      181 agggctttcc gctgctcacc accaagaagc tccatctgcg ctcgatcgtc atcgaattgc
      241 tctggttcct tcgcggcgag accaacatcg cctggctgaa ggagaacagg gtccggatct
      301 gggacgaatg ggcggacgag ggcggcgagc tcggcccggt ctacggcaag cagtggcgcg
      361 actgggaagg cccgggcggg gtccacgtcg accagatcgc caacctcatc gagctgatca
      421 accgccaccc cgcctcccgg cgccagatcg tctcggcctg gaacccggcc gagatcgagc
      481 gcatggcgct ggcgccgtgc cactgcctgt tccagaccca ggtcgcccgc ggccggctca
      541 acctccagct ctaccagcga agcgccgacg ccttcctggg agtgcccttc aacatcgcca
      601 gctatgcttt gctcacccac atgctggccc agcaatgcgg gcttgagccg ggcgtgttcg
      661 tctggaccgg cggggactgc catctttatt cgaaccacct ggaacaggcg cggcttcagc
      721 tgacccggga gccgaggccg ctgccccggc tcaacatcct ccgccgtccc gaagcgatcg
      781 atcgctatga atatgaggat ttcgagatca cggggtacga cgcccatccg cacatcaagg
      841 ccgaggtggc ggtgtgagcg ccccgattat cccggcggaa gccgggacct caggtcgaaa
      901 aggcacggcg ggtctccccg aggtcccggc tttcgccggg atgacggtgg actgaagtgc
      961 cgacaatcac cgtggtcgtc gcccgcgccc gcaacggagc gatcgggcgt cacggcaccc
     1021 tgccctggcg gctgcccgca gatctgaagc acttcaaggc ggtgaccatg ggcagcgcga
     1081 tggtgatggg gcgaaagacg ttcgacagcc ttcccggctt gctgccgggg cgtcgccaca
     1141 tcgtgctgac ccgcgacccg cggtggcggg cggagggggc ggagacggca aggtcggttg
     1201 acgaagtgat cgggctggcc gccgatgaac ccgtctccgt gatcggcggc gccgagatca
     1261 tcgccctgtt cctgccccgc gccgacaggg tcgagctgac cgaggtgcat gacgagcctg
     1321 aggcggacac gttcatgccc gcgttcgatc cggtggagtg gcgggaagtg tcgcgggcgg
     1381 accatccagg ctacagcttc gtaaggctgg agcggcggac ctgacccgca ccgtcaccct
     1441 gaacttggtt cagggtccag atctccaccc gcgaggcggc tcgccgatgg atgctgaaac
     1501 aagttcagca tgacgaggga cgggtgaaac ggctatgctc cgcccaccaa tccgggggga
     1561 tcatgcgcaa gttcatctac gggctcctcg ccctgatcgt ggtcgccgtc ggtgccttcc
     1621 tcatcctcgg acccggaatc gtcgaaagcg ggatgaacaa ggtggtggcg acgcccctgc
     1681 cgacgataag cgagcgggcg cgggcgcttc acccccgcct cgccgtcgcc gacatgcatg
     1741 cggacaccct gctgtggcag cgcagccttc tcgacccggc cagccgcggc caggtcgacc
     1801 tgccccggct cgaggcgggc aacgtcgcgc ttcaaatctt ctcctcggtc accaagacgc
     1861 ccaagggcca gaattacgac gcgaacggcg ccgacaccga caacatcacc ctgctcgccg
     1921 tcgcccagct tcagccgccg cgcacctgga attcgctgct tcagcgctcg ctctggcatt
     1981 ccgagaagct tcgccgcgcc gaagccggct cggaagggcg gctgaggatc gtcaggagcg
     2041 gaatcgacgt ccgaaggctg ctcgccgacc gcgccgcggg ccgaaaggtg gtcggggcac
     2101 tgctctcgat cgagggactc cagaatctgg agggcaggct cgacaatctc g
//
LOCUS       DASWNU010000157         6289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_31974, whole genome shotgun sequence.
ACCESSION   DASWNU010000157 DASWNU010000000
VERSION     DASWNU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31974"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VGB57_03070"
     CDS             complement(<1..646)
                     /locus_tag="VGB57_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0130360.1"
                     /translation="MPSDPAFYLFAIPAVILIGLAKGGFSGLGALGTPLMALGMDDPV
                     RAAAILLPILIAQDLVAVAAFRKNWDGSVLVAMLPGAAVGIGLGWLLAARISSDAVMA
                     ALGAISILFGAYRLVADSGGRIAASSSSPPWVGSLFGVAAGFTSQIAHAGGPPFQMWV
                     MPRRLDRDVFVGTSAIFFAIVNWIKVPAYFALGQFTRENALAAAALLPVAVLASL"
     gene            complement(804..2111)
                     /locus_tag="VGB57_03075"
     CDS             complement(804..2111)
                     /locus_tag="VGB57_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0130361.1"
                     /translation="MAETIAEGAPSSASRFTGGRVRWGICALLFFATTINYIDRQMIS
                     VLKPTLQGEYGWDESTYANIVFSFQLAYAIGYVVFGRLIDKLGARFGYSLAATIWGVA
                     AMAHALANNATHFMIARFALGLGESGNFPAGVKAVAEWFPKKERAFAIGIFNAGANIG
                     AILTPLIAPFLVVWFGWRAAFLFTGAIVFLWVVIWWTVYRRPREKKNLSPEELAYIES
                     DPADAPGDVKWLPLLRHRQTWAYALGKFMIDPIWWFFLFWLPSFFAQEHGLNLLTFGL
                     PLAAIYIISDVGSVGGGWLSSRLIHRGWSVNKARKTAMLVCALCVPPVMLAPGVESVW
                     GAVLLIGLATAAHQGFSANLYTIPSDLFPRKAVGAIIGIGGTIGAVGGMFMAKFTGWV
                     LDATGSYMPMFVIAGSSYLLALLVIHLLVPRMEEAKLGDEVRA"
     gene            2224..2733
                     /locus_tag="VGB57_03080"
     CDS             2224..2733
                     /locus_tag="VGB57_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130362.1"
                     /translation="MRNLLWPPVLYGLSACGQPAPEVRNESAANRVETSGKTDVPLAE
                     VPPEVLAAAAAAQPGFTPAEAEAETREGRRYFDVGGKLADGSEVEFDIMEEGGRWQVV
                     EAQRDISLTAAPEAVRKAAGSFPAGRVIESRQADGIVIYELYDAQQRKLEIKWNGKNA
                     EVLTTEWAH"
     gene            complement(2908..3720)
                     /locus_tag="VGB57_03085"
     CDS             complement(2908..3720)
                     /locus_tag="VGB57_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEY0130363.1"
                     /translation="MNLLTADPATIAGRFLAARRSAGGLADYPGDFPETLDDAYQVQD
                     QAIAAWGRPVIGWKVGRINPPLSDRYGADRLAGPIFACQNVADSGAAPECPVFAKGFA
                     AGEAEFLLRVARTPPRGKGSFTLEEAADLIDAVHVGIEIASSPLGAINKLGPVAVVSD
                     FGNNNGLVVGAAIPDWRSSGFEQWEVTTLLDGAEAGTGRAAAFPDGAIGAARFLFELM
                     AKRGIDLQPGQWISSGAVTGVHDARPGQRVEARFGDGYRVACVLRAARPEGL"
     gene            complement(3902..5269)
                     /locus_tag="VGB57_03090"
     CDS             complement(3902..5269)
                     /locus_tag="VGB57_03090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY0130364.1"
                     /translation="MSACGIVHFGPGAFHRAHQADYVDRLLKADPRWGIAAVSLRSAG
                     TIEALKRQRGRYTLAILDSEAGFRTLAAHKSFFGPGEGEGVRALLSDSAVRIVSSTVT
                     EKGYCLAGDGRLDFGHPDVVHDLADPDDPVSLVGWLALGLRDRRAAGLAPFTPLCCDN
                     MVANGKKLRSAVLDYAGRIDRELAGWIAGEAVFPDTMVDSITPATGEALRGLIRDRGF
                     DDEIPVSREAYSAWVIEDVLPPGSPDLKSVGAVMAADVGAWERAKLRILNGAHSTLAY
                     LGLLIGHESVAEAMADPVLAGFVERLVREDIIATLKPSPLDLQAYAGEILERFRNPAI
                     GHKLSQIAWDGSQKLPYRLLDPIADARAAGRPVERLAMPVAAWMLFVERQARAGVEIV
                     DPLAERLAQIGRRADPVAGMLELRQVFPERLAEDAVFRGAVEAAAAAIRGAGAHGALV
                     DSPSP"
     gene            complement(5266..>6289)
                     /locus_tag="VGB57_03095"
     CDS             complement(5266..>6289)
                     /locus_tag="VGB57_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY0130365.1"
                     /translation="DGVHAFAHPFGCSQLGDDLAGTRSVLAALACHPNAGGVLLVGLG
                     CESNQLDRLLEDIPEPMRGRVRTLKAQGVEDELEEGLALVDALVATAALSTRQPAGFG
                     ELVLGLKCGGSDGFSGLTANPLVGRMADRAIAAGGSAILSEIPEIFGAERLLMARARD
                     ERVFDSIVGLVNDFKRYFLASGQPVSENPSPGNIAGGITTLEEKSLGAVQKAGHAPVG
                     DVLRYGERVRRRGLTLLEAPGNDAVSSTALAAAGATVILFTTGRGTPLGFPVPTVKIA
                     SNSELAGRKKGWIDFDAGQVLNSGMELAADSLAHKIAAIASGEETAAERNGEREIAIW
                     KRGVTL"
BASE COUNT         1094 a         2138 c         2151 g          906 t
ORIGIN      
        1 cgaggctcgc aagcacggcg acaggcagca gcgccgcggc cgccagcgcg ttctccctcg
       61 tgaactggcc cagagcgaaa taggccggca ccttgatcca gttgacgatc gcgaagaaga
      121 tcgcggaggt gccgacgaag acgtcgcgat cgaggcgcct gggcatcacc cacatctgga
      181 agggcgggcc gccggcatgg gcgatctggc tggtgaagcc ggcggcgact ccgaacaggc
      241 tgccgaccca cggcggcgaa ctggaggagg cggcgatccg gcctccgctg tccgcgacga
      301 ggcgataggc gccgaacagg atcgagatgg caccgagcgc cgccatcacc gcgtcggacg
      361 agattcgcgc ggcgagcagc cagccgagcc cgatgccgac cgccgcgccg ggcagcatcg
      421 ccacgagcac cgagccgtcc cagttttttc ggaaggcggc gacggcgacc agatcctggg
      481 cgatcaggat cggaaggagg atggcggcgg ccctgaccgg atcgtccatg ccgagcgcca
      541 tcagcggcgt tccgagcgcg ccaaggccgg aaaagccccc cttcgcaagc ccgatcagaa
      601 taaccgccgg gatggcgaag aggtagaaag cgggatccga gggcatgtgc cgtctgcttc
      661 tcctaaattc ctccccgaga tttctcgggg agggggaccg cgcgaagcgc ggtggagggg
      721 ttctacggac tcggcagagc ccctccacca tgcttcgcat ggtccccctc ccctgcaaat
      781 gcaggggagg aatacaggtt cgctcaggcc ctgacttcgt cccccagctt cgcctcctcc
      841 atccgcggca cgagcaggtg gatgacgagc agggccagca aatagctcga gccggcgatc
      901 acgaacatcg gcatgtagct gccggtcgcg tcgaggaccc agccggtgaa cttggccatg
      961 aacatcccgc cgaccgctcc tatcgtcccg ccgataccga tgatcgcccc caccgccttg
     1021 cgcgggaaga ggtcggacgg gatcgtgtag aggttggcgg agaagccctg gtgggcggcg
     1081 gtggcgaggc cgatcagcag cacggcgccc cagacgctct ccacgcccgg cgcaagcatc
     1141 accggcggca cgcacagggc gcagaccagc atcgccgtct tgcgcgcctt gttgacgctc
     1201 cagccgcggt ggatgagcct ggacgacagc cagccgccgc cgacgcttcc gacgtcggag
     1261 atgatgtaga tggcggcgag cggcaggccg aaggtgagca ggttcagccc atgctcctgg
     1321 gcgaagaagc tcggcagcca gaacaggaag aaccaccaga tcgggtcgat catgaacttg
     1381 ccgagcgcat aggcccaggt ctggcggtgg cggagcaaag gcagccattt gacgtcgccc
     1441 ggagcgtcgg cgggatcgct ctcgatatag gcgagctcct ccggcgagag gttcttcttc
     1501 tcgcggggac ggcggtagac cgtccaccag atgacgaccc agaggaagac gatggcgccg
     1561 gtgaacagga aggcggcccg ccagccgaac cagacgacaa ggaaaggcgc gatcagcggg
     1621 gtcaggatcg ctccgatatt ggctccggcg ttgaagattc cgatcgcgaa ggcgcgctcc
     1681 ttcttcggaa accattcggc caccgccttg actccggccg ggaaattgcc ggattcgccg
     1741 aggcccagcg cgaagcgcgc gatcatgaaa tgggtggcgt tgttggccaa agcatgggcc
     1801 attgccgcga cgccccagat cgtcgccgcc agcgaatagc cgaagcgggc gccgagcttg
     1861 tcgatcagcc gcccgaacac gacgtagccg atcgcgtagg cgagctggaa ggagaagacg
     1921 atgttggcgt aggtggactc gtcccagcca tattcgccct gcagcgtcgg cttgaggacg
     1981 ctgatcatct gccggtcgat ataattgatc gtcgtcgcga agaagagcaa agcgcaaatg
     2041 ccccagcgca cccgcccgcc ggtgaacctg gacgcgctcg aaggcgcgcc ctccgcgata
     2101 gtctcggcca ttccaacacc ctccgtggcg cctttttggc gcttccttcg cgcttgtagg
     2161 ggaaaccggt gtcatataca atccgcctct gcggctcatg cgtccgccga ggaggggttt
     2221 ctaatgagaa atttgctttg gccgcccgtc ctttacggcc tctcggcctg cggccagccg
     2281 gcgcccgaag tgcgtaacga atcggccgcg aacagggtcg agacgtcggg caagaccgat
     2341 gtaccgctcg ccgaggtgcc gccggaagtg ctggcggcgg ccgcggcggc gcagccgggc
     2401 ttcacgcccg ccgaggccga ggccgagact cgcgagggcc gccgctattt cgacgtcggc
     2461 ggcaagctcg ccgacggttc ggaggtcgag ttcgacatca tggaggaagg cggacgctgg
     2521 caagtcgtcg aagctcagcg cgacatcagt ctcaccgccg cgccggaggc ggtgcgcaag
     2581 gcggcgggaa gcttcccggc cgggcgggtc atcgagagcc gccaggccga cgggatcgtc
     2641 atctacgagc tgtacgacgc gcagcagcgc aagctcgaga tcaagtggaa cgggaagaac
     2701 gccgaggtgc tgacaacgga gtgggcgcat taggtcctct tgcctcccag tcgccgaagg
     2761 cgatggggaa ccatgcggag catggtggag gggtagcaat ccggcctgag aggggccccg
     2821 ccggcttctc gggtgccgag ccaatacccc tccaccaccc ttcgggcggt ccccctcccc
     2881 atgcgttcgc atggggaggc aagtggttta cagcccctca ggcctcgccg cgcgaaggac
     2941 gcaggccacc cggtagccgt ccccgaagcg ggcttcgacg cgctgtcctg ggcgagcgtc
     3001 gtggactccg gtgactgcgc ccgacgagat ccactgcccc ggctggaggt cgatcccccg
     3061 cttcgccatc agctcgaaca ggaagcgcgc ggcgccgatc gctccgtcgg ggaaagcggc
     3121 ggcgcggccg gtgccggctt cggctccgtc gagcagggtc gtcacctccc attgctcgaa
     3181 gccgctcgag cgccagtcgg gaatggcagc accgacgacg aggccgttat tgttgccgaa
     3241 gtcggagacg accgcgaccg ggccgagctt gttgatcgcg ccgagaggcg agctcgcgat
     3301 ctcgatcccc acatggaccg catcgatcag gtcggcggcc tcctcgagcg tgaagctccc
     3361 cttcccccgc ggaggggtgc gggcgacgcg cagaaggaat tcggcctcgc cggcggcgaa
     3421 gcccttcgcg aagacggggc attcgggcgc cgccccggaa tcggcgacat tctggcaggc
     3481 gaagatcggg ccggccaggc ggtcggcgcc gtagcggtcc gacagaggcg gattgatccg
     3541 gccgaccttc cagccgatga cgggcctgcc ccaagccgcg atcgcctggt cctgcacctg
     3601 ataagcgtcg tccagcgtct ccgggaaatc gcccggataa tccgccaggc cgcccgcgga
     3661 gcggcgcgcc gcaaggaagc ggccggcgat cgtcgccgga tcggcagtca gcagattcac
     3721 ctcagcagcc cctcgaagtc tctttttttc cacgctaatg gaaaccggtg tcataggcaa
     3781 ggcggaagag gcgtttgtcg ggtgaaagac cctgggagcg tcgatcttcg tcatgctgaa
     3841 cttgcttcag catccagcca cgagccgcgg tgcgggtgga ggactggacc ctgaagcgag
     3901 ttcagggtga cggcgaatcg actaacgcac cgtgagcccc cgcccctcga atcgcggccg
     3961 cagcggcctc gaccgcaccg cgaaacaccg cgtcctccgc cagcctttcg ggaaagacct
     4021 gtcgaagctc gagcatcccc gcgaccggat ccgcgcggcg cccgatctgt gccagccgct
     4081 ccgccagcgg gtcgacgatc tccacccctg cccgcgcctg ccgctcgacg aacagcatcc
     4141 aggccgcgac cggcatcgcg agccgctcca ccggccgtcc cgccgcccgc gcgtcggcga
     4201 tcgggtcgag cagccggtag ggcagcttct gcgagccgtc ccaggcgatt tgcgacagct
     4261 tgtggccgat ggcgggattg cggaaacgct ccagtatctc gccggcatag gcctgcaggt
     4321 cgaggggcga gggcttcaag gtggcgatga tgtcctcgcg caccagccgc tcgacgaagc
     4381 cggcgaggac cgggtccgcc atggcctcgg ccaccgactc gtgcccgatg agaagtccga
     4441 gataggcgag ggtggaatgg gcgccgttca ggatccgaag cttggcccgt tcccatgcgc
     4501 cgacgtcggc cgccatgacc gcgcctaccg acttcaggtc cggcgatccg ggcggaagca
     4561 cgtcctcgat cacccaggcc gaataggctt cgcggctgac ggggatttcg tcgtcgaagc
     4621 cccggtcgcg gatcaggccg cgaagcgcct cccccgtcgc cggggtgatc gaatcgacca
     4681 tcgtgtcggg gaagaccgcc tcgccggcga tccaccccgc cagctcgcgg tcgatgcgcc
     4741 cggcataatc gagcacggcc gagcgcagct tcttgccgtt cgcgaccata ttgtcgcagc
     4801 aaagcggagt gaacggcgcc agccccgccg cgcgccggtc gcgaagaccg agggcgagcc
     4861 agccgaccag gctcacggga tcatctggat ccgcgagatc gtggacgacg tcgggatggc
     4921 cgaagtcgag cctgccgtcg ccggcgaggc aatagccctt ctcggtgacg gtgctggaga
     4981 cgatccgaac cgccgagtcg ctcaaaagcg ctcgaacccc ctccccttcc cctggcccga
     5041 agaagctctt gtgcgcggcg agggtccgga agccggcttc cgaatcgagg atggcgagcg
     5101 tgtagcgccc ccgctgccgc ttcagcgcct cgatcgtccc cgccgagcgc agcgacaccg
     5161 cggcgatccc ccagcgggga tccgccttca gaagcctgtc gacataatcg gcctggtggg
     5221 cgcggtggaa ggcgcccggt ccgaaatgga cgataccgca agcgctcaca aagtgacgcc
     5281 gcgcttccag atcgcgatct cgcgctcccc gttgcgctcg gccgccgtct cctcgcccga
     5341 ggcgatggcg gcgatcttgt gcgcgagcga atcggcggcg agctccattc cgctattcag
     5401 gacctgcccg gcgtcgaagt cgatccaccc cttcttccgg ccggcgagct cgctgttgga
     5461 ggcgatcttg accgtcggca ccgggaagcc gagcggagtt ccgcggccgg tggtgaacag
     5521 gatgacggtc gctccggcgg cggcgagcgc ggtcgaggag acggcgtcgt tgccgggcgc
     5581 ctcgagcagg gtcagcccgc gccgccgcac ccgctcgccg tagcgaagca cgtcgccgac
     5641 cggtgcgtga cccgccttct ggaccgctcc gagcgacttc tcctcgagcg tggtgatccc
     5701 cccggcgata ttgccgggcg acggattctc cgagaccggc tggccgctgg cgaggaaata
     5761 gcgcttgaag tcgttgacca ggccgacgat cgaatcgaac actcgctcgt cgcgcgcccg
     5821 cgccatcagc agccgctcgg cgccgaatat ttccggtatc tcgctgagta tggcgcttcc
     5881 gccggccgcg atggcgcggt ccgccatccg gcccaccagc gggttggcgg tgaggccgga
     5941 gaagccgtcc gagccgccgc atttgaggcc cagcaccaat tcgccgaacc cggccggctg
     6001 gcgcgtcgac aaagcggcgg tggcgaccag cgcgtcgacc agggccagcc cctcctcgag
     6061 ctcgtcctcg acgccctggg ccttcagagt ccgcacgcgc ccgcgcatcg gctcgggaat
     6121 gtcctcgagc agccggtcga gctggttcga ttcgcacccc aggccgacga gcaggacgcc
     6181 gccggcattt gggtggcagg ccagagccgc gagcaccgaa cgggtgccgg caaggtcgtc
     6241 gccgagctgc gagcagccga acggatgggc gaaggcatgg accccgtcg
//
LOCUS       DASWNU010000158         6274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_160913, whole genome shotgun sequence.
ACCESSION   DASWNU010000158 DASWNU010000000
VERSION     DASWNU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160913"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..246
                     /locus_tag="VGB57_03100"
     CDS             43..246
                     /locus_tag="VGB57_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY0130366.1"
                     /translation="MASDPSVPEISYVTTPRVACDGASETQAALGHPRVYLQIDEKGY
                     VDCGYCDKRFVLKGRPADEGSEG"
     gene            443..1048
                     /locus_tag="VGB57_03105"
     CDS             443..1048
                     /locus_tag="VGB57_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130367.1"
                     /translation="MHKLTTSTALAGLLLLGLAACDSAPTANDAAANSAANAAVTANA
                     SAAQPPSAPVAAAPIVLEGAGLRIPGASPPRTLAFDMPEPATIEALTTALGRPPTELG
                     ENEECGGGGMKFAEWKDELTVWFLDGRFAGWDSEGKLKTIEGIGIGSSRADVAGLPGF
                     EVEESTLGTEFRAGGLSGILDSKAPAAKVEHLWGGATCVFR"
     gene            1033..1539
                     /locus_tag="VGB57_03110"
     CDS             1033..1539
                     /locus_tag="VGB57_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4087 domain-containing protein"
                     /protein_id="HEY0130368.1"
                     /translation="MRLPLGAALAILLAAAGCSPGSGPVGNGQASRAEAAAEPAPSDA
                     IAAAAAPAPAAAAAVAPSRSAAASPAKGARRCGWLSNPTPANWWLTDSEGQWILGTQG
                     ADQAPGMDDMPDMSTAGWVETNGHYGYGCACITITADADGRVTRIADAQPKPLKQCRA
                     DRKLPKPE"
     gene            1560..2072
                     /locus_tag="VGB57_03115"
     CDS             1560..2072
                     /locus_tag="VGB57_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130369.1"
                     /translation="MGLFDWLTGNKPAPSGVKRQSAAKLKAGLMGVNRKSAPFKVRSG
                     KEEDVDLVAEWKIVDAIWYEIFAKAGIERVFSVLMKFDERKGEVRAVDQEWEVEWRAG
                     EPILSLKGSAFRGRKWEKGFETVWAFREDGSFGEIYDYRFDTSEIHKPLIAAAHKAGW
                     GWRSVAFGKL"
     gene            2194..3621
                     /gene="tldD"
                     /locus_tag="VGB57_03120"
     CDS             2194..3621
                     /gene="tldD"
                     /locus_tag="VGB57_03120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY0130370.1"
                     /translation="MTYTDPRHFLYRGDALTPDSALRLTADALQSCDDGELFLQYSVS
                     ESFGFDDGRLKMADFDTKAGFGLRGVSGETSAFAHANELSEAAIRRAGETMAVLDPTR
                     GPRPGPPRRTNAALYTDSDPLSLIPFARKVALCQEIDAAARARDPRVVQVTVALAGVW
                     SVIEIVRPDGFVAHDVRPQVRLNVQVVVEKNGRRESGNHGLGGRYLYDRLFEPATWNR
                     VIDIALAQALVNLESIAAPAGEMTVVLGPGWPGVLLHEAIGHGLEGDFNRKGTSAFSG
                     RIGERVAAPGVTVVDDGSLADRRGSLSIDDEGTPTQCTTLIEDGILKGYIHDRLNARL
                     MGVEPTGNGRRQSFAHAPMPRMTNTFMLGGQEDPGEILARAGNGIYAKSFGGGQVDIT
                     SGKFVFSCTEAYLIGNGKLGAPIKGATLIGDGPTVLTRVAGIGNDMALDEGVGICGKG
                     GQSVPAGVGQPTLLIDGITVGGTAA"
     gene            3621..4154
                     /locus_tag="VGB57_03125"
     CDS             3621..4154
                     /locus_tag="VGB57_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY0130371.1"
                     /translation="MAWQDEVLAFWFGLAREQWWKADPALDAEIGDRFLALWEEQRAN
                     VAEAFLGSPRDSIAAIVLFDQFPRNMHRGHADQFSTDPLALAIARGAVDRGLDEAMSP
                     AERGFLYMPFQHSERIEDQKRSVALFTALGDEYQLDFAKKHHDVIQRFGRFPHRNAIL
                     GRPPRPAEVEAGDVVPW"
     gene            4154..4357
                     /locus_tag="VGB57_03130"
     CDS             4154..4357
                     /locus_tag="VGB57_03130"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY0130372.1"
                     /translation="MALVEAAKFYDSLAAGAAQSLLSDKGIDSFIFDMGMTLQGVGFA
                     VPVRLMVDEDDLENAERLLAEGP"
     gene            4433..5416
                     /locus_tag="VGB57_03135"
     CDS             4433..5416
                     /locus_tag="VGB57_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0130373.1"
                     /translation="MNTAAGNPAPSEFIDPGLRRDDEGAPVLELLNLSKTYGRRGGGV
                     RAVRDVSLSVGAGEVYGFLGPNGAGKSTTIRMLIGLISPSSGEARLFGEDLRSNPAVL
                     RRVGSLVDGGNFYPFLSGRRNLEVLAKTQGEGAERIDGLLDQVGLAKDGKRKVKGYST
                     GMRQRLGVAAALLGDPDLVILDEPVNGLDAAGIQEMRALIRGLAVDRGKTVFLSSHLL
                     HEVQQVCDRVAIIARGEIVRESTIAELLRQDDGIRIEAAPAEIAEAALASRWAVERDG
                     DALIVRATRAEAPEVVRRLVGAGADLFRLAPEQRNLEEVFLSITQPEADDA"
     gene            5409..6257
                     /locus_tag="VGB57_03140"
     CDS             5409..6257
                     /locus_tag="VGB57_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130374.1"
                     /translation="MLEALSAEALKMRTHKATWFLVWLFPIAFTIIMLLMIGAGMMGF
                     DPPEQPNVHSWLENTTLIWFIPNNAFGRYLISAFVAVVFAGEYGWNTWKLIVPHRRRA
                     SLIAAKYALSVLLLAISFLLTAALITGLTFADDALTGDTIPAGITAAVLWEAHSESAL
                     AAMLPILIAVGYASLAAILTRSTIAALVIAIAATTLEQVIVGYGPQLYLNFPSIVWPL
                     YNVLPGHHLANISDCIREGAALKRGFPGGLVVHYSCLASLGILAAWIAALWGATFAAF
                     SRQDIN"
BASE COUNT         1001 a         2206 c         2099 g          968 t
ORIGIN      
        1 ccgtcgcttg acgggcgagg cccgcccgct aaggccaagg ccatggccag cgacccttcc
       61 gtccccgaga tcagctacgt caccacgccg cgcgtcgcct gcgacggcgc gagcgagacg
      121 caagccgccc tcggccaccc ccgcgtctac ctgcagatcg acgagaaggg ctatgtcgac
      181 tgcggctatt gcgacaagag gttcgtcctg aagggccggc ccgcggacga gggctcggag
      241 ggctaggccc tgcaccgccg tcatgctgaa cttgtttcag catccagcgt cgagctgcgg
      301 gcgggtggag aactggaccc tgagacagtc cagggcgatg cctggaggac tcacgccggc
      361 ttcccgcctc ccgttcgcca gccgttgcca tcggcgcgta caaggtccaa tccggcggac
      421 gaccactgaa aagggcctcg acttgcacaa actgacgacc agcaccgccc ttgcgggcct
      481 gctcctgctc gggcttgccg cctgcgattc cgcgccgacc gcgaacgacg cggcggccaa
      541 ttcggcggcg aatgccgccg tgaccgccaa tgcctccgca gcgcagccgc cgtcggcacc
      601 cgtcgccgcc gcgccgatcg tcctggaagg cgctgggctg cgcatccccg gcgcctcgcc
      661 gccgcgcacc ctggccttcg acatgcccga gccggcgacg atcgaggctc tgaccacggc
      721 gctcgggcgc cctcccaccg agcttggcga aaacgaggaa tgcggcggcg gcggaatgaa
      781 gttcgccgag tggaaggacg agctcaccgt ctggttcctc gatggccgct tcgccggctg
      841 ggactccgag ggcaagctga agaccatcga gggcatcggc atcggctcga gccgcgccga
      901 tgtcgccggc ttgccggggt tcgaggttga ggagagcacg ctcggaaccg agttccgggc
      961 cggcgggctg agcggaatcc tcgactccaa ggcccctgcc gcgaaagtcg agcatttgtg
     1021 gggaggcgcc acgtgcgtct tccgctaggc gccgcgctcg ccatcctgct cgccgccgcg
     1081 ggctgctcgc ccggctccgg gccggtgggc aatgggcaag cgtcccgagc ggaggcggca
     1141 gccgaacctg cgccttcgga cgcgatcgct gccgcggccg ctccagcgcc cgccgccgcc
     1201 gccgccgtcg cgccctcccg ctccgcggcg gcgagcccgg cgaagggcgc gcgccgctgc
     1261 ggctggctct ccaatcccac tccggccaat tggtggctca ccgactccga aggccagtgg
     1321 atcctcggga cccagggcgc cgaccaggcg ccggggatgg acgacatgcc cgacatgagc
     1381 acggccggct gggtcgagac caacggccat tacggctatg gctgcgcctg cattacgatc
     1441 accgccgatg ccgacggacg tgtgacccgg atagccgacg cccagccgaa gccgctgaag
     1501 cagtgccggg ccgaccggaa gctgccgaag ccggagtgag gtgccgagag ggggacgaga
     1561 tgggtttgtt cgactggctg accggcaaca agcccgcccc ttccggggtg aagcggcagt
     1621 cggcggcgaa gctcaaggcc gggctgatgg gcgtcaaccg caagtcggcg ccgttcaagg
     1681 tgcggagcgg caaggaggaa gacgtcgacc tcgtcgccga atggaagatc gtcgacgcga
     1741 tatggtacga gattttcgcc aaggcgggca tcgagcgggt gttctcggtg ctgatgaagt
     1801 tcgacgagag gaagggcgag gtccgcgccg tcgaccagga atgggaagtg gaatggcggg
     1861 cgggggagcc gattctctcg ctcaagggat cggccttcag gggccgcaag tgggagaagg
     1921 gcttcgagac cgtctgggcg ttccgcgagg acggctcgtt cggcgagatc tacgattatc
     1981 gcttcgacac gagcgagatc cacaagcccc tcatcgcggc ggcccacaaa gcgggctggg
     2041 gatggcggag cgtcgcgttc gggaagctgt gacggcgcag ccgtcatccc ggcgaacgcc
     2101 gggatctcgg ggagacccgc cgtgactctt cctcgctgag gtcccggctt tcgccgggat
     2161 gacggggagg atccgagtcc ctatatccct tacatgacct acaccgatcc ccgccacttc
     2221 ctctaccgcg gcgacgccct gactccggac agcgcgctcc gccttaccgc cgatgcgctc
     2281 cagtcctgcg acgacggcga gcttttcctc caatatagcg tctccgaatc cttcggcttc
     2341 gacgacggcc ggctgaagat ggcggatttc gacaccaagg cggggttcgg gcttcgcgga
     2401 gtctcgggcg agaccagcgc tttcgcccat gccaacgagc tttcggaagc cgccatccgc
     2461 cgcgccgggg agacgatggc ggtgctcgat ccgacccggg ggcctcggcc cggtcccccg
     2521 cgccggacca atgcggcgct ctataccgac tccgacccgc tcagcctcat ccccttcgcc
     2581 cgcaaggtcg ccctctgcca ggagatcgac gccgccgccc gggcccgcga cccgcgggtc
     2641 gtccaggtca cggtggcgct cgccggcgtc tggtcggtga tcgagatcgt ccgcccggac
     2701 ggcttcgtcg cccacgacgt ccggccgcaa gtgcgcctca acgtccaggt cgtcgtcgag
     2761 aagaacggcc ggcgcgagag cggcaatcac ggcctcggcg gccgctatct ctacgaccgg
     2821 ctgttcgagc cggcgacctg gaaccgggtg atcgacatcg cgctcgccca ggcgctggtg
     2881 aacctcgaat cgatcgcagc gccggcgggc gagatgacgg tcgtgctcgg accgggctgg
     2941 cccggagtgc ttctccacga ggccatcggc cacggcctcg aaggcgattt caaccgcaag
     3001 ggcacctccg ccttttccgg ccggatcggc gagcgggtgg cggctccggg agtcaccgtc
     3061 gtcgacgacg gctcgctcgc cgaccgccgg ggctcgctgt cgatcgacga cgagggcact
     3121 cccacgcagt gcaccaccct gatcgaggac gggatcctca agggctatat ccacgatcgc
     3181 ctgaacgccc ggctgatggg ggtcgagccg accggcaacg gccgtcgcca gagcttcgcc
     3241 cacgcgccga tgccgcggat gaccaacacc ttcatgctcg gcgggcagga ggatccgggc
     3301 gagatcctgg cccgggcggg gaacggcatc tacgccaaga gcttcggcgg cggccaggtc
     3361 gacatcacca gcggcaagtt cgtgttctcc tgcaccgagg cctatctgat cgggaacggc
     3421 aagctcggag cgccgatcaa gggcgcgacc ctgatcggcg acgggcccac cgtcctgacc
     3481 agggtcgccg gcatcggcaa cgacatggcg ctcgacgaag gagtcggcat ttgcggcaag
     3541 ggcggtcagt cggttccagc cggagtcggc cagcccaccc tgctgatcga cggcatcacg
     3601 gtggggggta cggccgcctg atggcctggc aggacgaggt cctcgccttc tggttcggcc
     3661 tcgccaggga gcaatggtgg aaggccgatc cggcgctcga cgccgagatc ggggatcgct
     3721 tcctggccct gtgggaggag cagcgggcga atgtggccga ggccttcctc ggcagcccgc
     3781 gggactcgat cgcggcgatc gtcctgttcg accagttccc gcgcaacatg catcggggcc
     3841 atgccgacca attctccacc gatccgctcg ccctggcgat cgccaggggc gcggtcgacc
     3901 ggggcctcga cgaggcgatg agcccggcgg agcgcggctt cctctacatg cccttccagc
     3961 atagcgagcg gatcgaggac cagaagcgct cggtcgcgct cttcaccgcc ctcggcgacg
     4021 agtatcagct cgacttcgcg aagaagcatc acgacgtcat ccagcgcttc ggccgattcc
     4081 cgcaccgcaa cgcgatcctc ggccgcccgc cgcggccggc ggaggtcgag gccggcgacg
     4141 tggtgccctg gtaatggcgc tggtcgaggc ggccaaattc tacgacagcc tggccgccgg
     4201 ggcggcgcag tccctgttgt ccgacaaggg gatcgacagc ttcatcttcg acatgggcat
     4261 gacccttcag ggcgtcggct tcgccgtgcc ggtgcggctg atggtggacg aggacgatct
     4321 cgagaacgcg gagcgcctgc tggccgaggg gccttgacag ccaaggtgta ttaattcaat
     4381 aatacaccat gagcaattcc ggaaaccgtc atcccggcga aggccgggaa ccatgaacac
     4441 ggccgctgga aatccggcac catccgagtt catagatccc ggccttcgcc gggacgacga
     4501 gggagctccc gtcctcgagc tcctgaatct cagcaagacc tacgggcgcc gcggcggcgg
     4561 ggtccgggcg gttcgcgacg tcagcctctc cgtcggggcg ggagaggtct acggcttcct
     4621 cgggccgaac ggcgccggca agtcgacgac gatccggatg ctgatcggcc tcatttcacc
     4681 gtcctccggc gaggcccggc tgttcggcga ggatttgcgc tccaacccgg ccgtccttcg
     4741 ccgggtcggc tcgctggtcg acggcggaaa tttctacccc ttcctctccg gccgacggaa
     4801 cctcgaggtc cttgcgaaga cccagggcga aggcgccgaa cggatcgacg gcctgctcga
     4861 ccaggtcggg ctggcgaagg atgggaagcg caaggtgaaa ggctattcga cggggatgcg
     4921 gcagcgcctc ggagtcgccg cggccctgct cggcgatccc gatctcgtca tcctcgacga
     4981 gccggtcaac ggcctcgacg ccgccgggat ccaggagatg cgcgccctca tccgcggcct
     5041 cgccgtcgac cggggcaaga ccgtcttcct ctccagccac ctcctccacg aagtccagca
     5101 ggtctgcgac cgggtcgcga tcatcgccag gggcgagatc gtccgcgaat ccacgatcgc
     5161 cgagcttctg cgccaagacg acggcattcg aatcgaggcc gcgcccgccg aaatcgccga
     5221 ggccgccttg gcctcgcgct gggcggtcga gcgcgacggc gacgccctga tcgtccgggc
     5281 gacccgggcg gaggctccgg aagtggtccg ccgcctcgtc ggagccggcg ccgatctctt
     5341 ccggctggcg cccgagcagc gcaacctcga ggaggtcttc ctctccatca cccagccgga
     5401 ggcggacgat gcttgaggcc ctttccgccg aagcgctcaa gatgcgcacc cataaggcga
     5461 cctggttcct cgtctggctg ttcccgatcg ccttcaccat catcatgctg ttgatgatcg
     5521 gcgcgggcat gatggggttc gacccgcccg agcagccgaa cgtccatagc tggctcgaaa
     5581 acacgacctt gatctggttc atccccaaca atgccttcgg ccgctacctc atctccgcct
     5641 tcgtggccgt cgtcttcgcc ggcgaatatg gctggaacac ctggaagctg atcgtgccgc
     5701 accggcgccg cgcttcgctc atcgccgcca aatatgcgct gtccgtcctc ctgctcgcga
     5761 tcagcttcct gctgaccgcc gcactcatta ccggcctgac cttcgccgac gacgctctga
     5821 cgggagacac gatcccggcc ggaatcacgg cggccgtgct ctgggaggcg cacagcgagt
     5881 ccgcgctcgc cgcaatgctg ccgatcctga tcgcggtcgg ctatgcgagc ctcgccgcga
     5941 tcctgacccg ctcgacgatc gccgcgctcg tcatcgccat cgccgcgacg accctggagc
     6001 aggtcatcgt cggatacggt ccgcagctgt acctcaattt cccgtcgatc gtctggccgc
     6061 tctacaatgt gcttccaggc caccacctcg ccaacatctc cgactgcatc cgcgagggcg
     6121 cggctctgaa gcggggcttc cccggcggac tcgtggtcca ctattcctgc ctggcctccc
     6181 tcggcatcct cgccgcgtgg atcgccgctt tgtggggagc gaccttcgcc gccttcagcc
     6241 gtcaggacat caactagagc cgggcgcgga tcgg
//
LOCUS       DASWNU010000159         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_32004, whole genome shotgun sequence.
ACCESSION   DASWNU010000159 DASWNU010000000
VERSION     DASWNU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32004"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGB57_03145"
     CDS             complement(<1..507)
                     /locus_tag="VGB57_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0130375.1"
                     /translation="MMDPERLPFVVQRDPDGERSFAALLGLVREKEGFLGERLLTHGA
                     ILLRGWPVRSCEQFAELVEGLSGRGGLLQYRGGASPRRALTAAAHPVYNSTEYPPDME
                     LSLHNELSYSSAWPDRIYFLCMTAPADGGETTLGDSRRILAGMPTGVRARFEAKGVRY
                     IRNLPSGAG"
     gene            636..818
                     /locus_tag="VGB57_03150"
     CDS             636..818
                     /locus_tag="VGB57_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130376.1"
                     /translation="MATQPEIAPPDTVQPQSPPESPSFEPPVEEPGTGGPEIVPEQPD
                     QDSPGGSPAETPPPPD"
     gene            1017..1478
                     /locus_tag="VGB57_03155"
     CDS             1017..1478
                     /locus_tag="VGB57_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0130377.1"
                     /translation="MAILPFLFMSSRNDDRDWQLNTALQAARAAEEALDEPRNCRKRV
                     AWLLCETGAQYARWTGECSDHVPLSRQEIAAALGVSVCRVKRVMALLSLSGVVASEPR
                     GLSILDWRRLCAIAGYDPGRLGFEVSEELTAAPDEEAPMLVTAAGDPAYFE"
     gene            complement(1485..2630)
                     /locus_tag="VGB57_03160"
     CDS             complement(1485..2630)
                     /locus_tag="VGB57_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HEY0130378.1"
                     /translation="MPQIDAGYLRAQLERLLSIPSPTGYTDTIVRYVSEELERLKVPY
                     SVTRRGAILALYRGREVEGARAVVAHLDTLGATVKMIKDNGRLELVSIGTWSARSAET
                     TRCTIFTEEGSYRGTILPLKASGHTFNEEVDTQPVGWRYVEVRIDADCSDRASVEALG
                     IHIGDFVAIDSGAEFLDNGFIVSRHLDDKAGCASALAALKALVDNRVPVPVDIHFIFT
                     ITEEVGSGASAVLLDNVVAMVSIDNGTTAPGQNSAEFGVTIGMADQVGPFDYHLTRKL
                     IRLCEENGIRHQRDVFRYYRSDSASALEGGADVRTALITFGIDASHGYERIHLHALKS
                     IAELVCAYAASPVEIARDLEELTGLEGFTEQPTEPAAHSVDDPSAHE"
BASE COUNT          423 a          900 c          897 g          450 t
ORIGIN      
        1 gcccgcgccg gagggcaggt tgcggatgta gcgcaccccc ttcgcctcga accgggcgcg
       61 gacgccagtg ggcattccgg cgaggattcg gcggctgtcg cccagggtcg tctcgccgcc
      121 gtccgcgggg gccgtcatgc agagaaagta gatgcggtcg ggccaggcgc tggaatagga
      181 gagctcgttg tgaagcgaga gctccatgtc cggcggatat tcggtggagt tgtagaccgg
      241 atgcgctgcc gcggtgagcg cgcgccgggg cgaggcgccg ccccgatact ggagcaagcc
      301 tccgcggccc gacagccctt ccaccagctc cgcgaactgt tcgcagcttc gaacgggcca
      361 gccgcgcagc aggatcgcac cgtgcgtcag cagccgctcg ccaagaaagc cctctttttc
      421 gcggaccagc ccgagcaacg ccgcgaagct tcgctcgccg tctgggtcgc gctggacgac
      481 gaacggaagg cgctctgggt ccatcatccg tgacgcaacg cggctcgggc cggaccgtgc
      541 cgcggatgcg agcaaaatcc cggctcccgc cctcaggcct ttgggaaacg agaggcggtt
      601 cgagggttgg gaggtgagca aagaggagaa gaagcgtggc gacccaaccc gaaatcgctc
      661 cccccgacac cgttcagccg caatcgcctc cagaaagccc gtcgttcgaa ccgcccgtcg
      721 aggagccggg gaccggcgga ccggagatcg tgccggagca gccggatcag gactcccccg
      781 gcggatctcc ggccgaaacc cctccgcctc ccgattgaac ccggcggcga gcgcctgtcg
      841 cccctgcccg agcttgatcc tgaccacggc cgcggttccc ggcctaatct tcgttaacgt
      901 gtttttcagg aacggaagcc gccttcaagc gcttgatttg acattggttt tctcaacttt
      961 tccgaggtct tgcggcgatg gccacacgcg tcgaaatgcc ttctctttcc ccgtctttgg
     1021 ccattcttcc cttcctcttc atgtcgtccc gcaatgacga tcgcgactgg cagctcaaca
     1081 cggccctgca agcggcgcgc gcggccgaag aagccctgga cgagccgcgc aactgccgga
     1141 aacgggtggc ctggctcctt tgcgaaacgg gcgcccaata tgcccgctgg accggcgagt
     1201 gctccgatca cgtcccgctg tcgcgccagg agatcgcggc ggcgctcgga gtcagcgtct
     1261 gccgcgtgaa gcgggtcatg gcgctgttgt cgctctccgg ggtcgttgcc tcggagccgc
     1321 gcggattgag catcctcgac tggcgccgcc tgtgcgccat cgccggctac gatcccggcc
     1381 gcctcggctt cgaagtttcg gaggagctga cggcggcgcc ggacgaggag gcgccgatgc
     1441 tcgtcaccgc cgccggcgac cccgcatatt tcgagtgaac ccggtcattc gtgcgcggac
     1501 ggatcgtcga cgctgtgcgc cgccggctcc gtgggctgct cggtgaagcc ttccagcccc
     1561 gtcagctcct ccagatcgcg ggcgatttct accgggctgg cggcataggc gcaaacgagc
     1621 tcggcgatcg atttcagcgc atgaaggtga atccgctcgt agccgtggct cgcgtcgatt
     1681 ccgaacgtga tcaaggccgt ccgcacgtcc gcgccgcctt cgagcgccga ggcgctgtcg
     1741 gagcggtaat aacggaagac gtcgcgctgg tgccggattc cgttttcctc gcacaggcga
     1801 atgagcttgc gggtgaggtg atagtcgaaa gggccgacct ggtcggccat cccgatcgtc
     1861 actccgaatt ccgcgctgtt ctgcccgggc gcggtggtgc cgttgtcgat cgagaccatg
     1921 gcgacgacgt tgtcgaggag gacggccgaa gcgccggatc cgacttcctc ggtgatcgtg
     1981 aagatgaagt ggatgtcgac ggggaccggg acccggttgt cgacgagcgc cttcagcgcc
     2041 gccagggccg aggcgcatcc cgctttgtcg tcgaggtggc gtgagacgat aaagccgttg
     2101 tccaggaact cggcgcccga gtcgatggcg acgaaatcgc cgatatggat gcccagcgcc
     2161 tcgacgctcg cccggtcgct gcaatcggcg tcgatcctga cctcgacgta gcgccagccg
     2221 accggctggg tgtcgacctc ctcgttgaac gtgtggcccg aggccttgag gggcaggatc
     2281 gtgccccggt agctgccctc ctcggtgaag atggtgcagc gcgtcgtctc cgccgaccgg
     2341 gccgaccagg tgccgatcga caccagctcc agccggccgt tgtccttgat catcttcacc
     2401 gtggcgccga gcgtgtcgag atgcgcgacc accgcccggg caccctcgac ctcgcgcccc
     2461 cggtagagcg cgagaatggc gccgcgacgg gtcaccgaat acggcacctt caggcgctcc
     2521 agctcctcgg agacgtagcg gacgatggtg tcggtgtagc cggtggggct cgggatggag
     2581 agcagccgct caagctgagc cctcaaatag ccggcgtcaa tctgcggcaa tgtccgccct
     2641 cctcccggcc tgcgccgcgg ccgccatggt
//
LOCUS       DASWNU010000160         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_394661, whole genome shotgun sequence.
ACCESSION   DASWNU010000160 DASWNU010000000
VERSION     DASWNU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394661"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VGB57_03165"
     CDS             <1..915
                     /locus_tag="VGB57_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0130379.1"
                     /translation="TGKEIIAQAIHAASPRGKKPLHIVNCGAIPQNLVESVLFGHEKG
                     SFTGAFDRHVGRFEDADGSTLFLDEVGELPLETQVKLLRALETGEIQRIGGRAIHMVD
                     VRIIAATNRRLAEEVARGRFREDLYYRLNVVHVPIPPLRDRASDIPPLARHLLARIAE
                     QPGMRHLSITDDGLEVLMRYGWPGNVRQLQNALFRAAVLCDGNALTATDFPQIAQEST
                     LSRRASDYHRPALNGASQQAALSNGPGITLYDPDGNLRPLEAIEADVIRLAIGHYRGR
                     MTEVARRLGIGRSTLYRKLGELGIGEVA"
     gene            917..1348
                     /locus_tag="VGB57_03170"
     CDS             917..1348
                     /locus_tag="VGB57_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130380.1"
                     /translation="MNPTDRPAWPWRLLAWVLLVLGAAISLPALLVTPISGLVYIVLA
                     WTALGGVLAYAYGRRARPLWFWRIFAPLLSLYTMWDLGGHSGRFVAALRNGPEMSGAH
                     ISIFVVAVPLNILLCIALLRHAQLLRGGQRSAMRDLEAVFA"
     gene            1455..>1868
                     /locus_tag="VGB57_03175"
     CDS             1455..>1868
                     /locus_tag="VGB57_03175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0130381.1"
                     /translation="MPDFTQAIVLITGAASGIGAATATRLAEAGARKLILMDRDEDKL
                     RDFAFTLPCERQIRIGDVADERLWEREDLTGLTHAVVNAGIGAGGPIADTSFADWRQV
                     MSINLDGAFLTLQAAMKAMRDTGGSIVLTASVAGVK"
BASE COUNT          282 a          716 c          577 g          293 t
ORIGIN      
        1 accggcaagg aaatcatcgc ccaggcgatc cacgccgcct ccccgcgcgg caagaagccg
       61 ctccacatcg tcaattgcgg cgccatcccc cagaatctgg tcgaatccgt cctgttcggc
      121 cacgagaagg gcagcttcac cggcgccttc gaccgccatg tcggccgctt cgaggatgcc
      181 gacggatcga ccctgttcct cgatgaggtc ggcgagcttc cgctcgagac ccaggtcaag
      241 ctgctccgcg ccctcgaaac cggcgagatc cagcgcattg gcggccgcgc catccacatg
      301 gtcgacgtca ggatcatcgc ggcgaccaac cggcgcctcg ccgaggaagt cgcccgcggc
      361 cgcttccgcg aggacctcta ctaccgactc aacgtcgtcc acgttccgat cccgcctctg
      421 agggaccggg ccagcgacat cccgccgctc gcccgccacc tcctcgcccg gatcgccgag
      481 cagcccggaa tgcggcatct ctcgatcacc gacgacggcc tggaagtgct gatgcgctac
      541 ggctggccgg gcaacgtccg ccagctccag aacgccctct tccgagccgc cgtgctctgc
      601 gacggcaacg ccctgaccgc caccgacttc cctcagatcg cccaggaatc gaccctctcg
      661 cgccgggcca gcgactatca tcgcccggcg ctgaacggcg cctcccagca ggcggccctc
      721 tccaacggcc cgggcatcac cctctacgac cccgacggca acctccgccc cctggaagcg
      781 atcgaggccg acgtcatccg cctcgccatc ggccattatc gcggccggat gaccgaagtg
      841 gcgcggcggc tcgggatcgg gcgctcgacg ctctaccgca agctcggcga gctcggcatt
      901 ggggaagtcg cctgaggtga acccgacaga tagaccggcc tggccgtggc gcctgctcgc
      961 ctgggtcctg ctcgtcctgg gcgcagcgat cagcctcccc gccctgctgg tgaccccgat
     1021 ctcggggctg gtctacatcg tcctagcctg gaccgccctc ggcggggtgc tggcatacgc
     1081 ctatggccgc cgggctcgac cgctgtggtt ctggcggatt ttcgcccccc tgctcagcct
     1141 ctacacgatg tgggatctcg gagggcattc gggccgcttc gtggcggccc ttcgcaacgg
     1201 cccggagatg agcggcgccc atatctccat cttcgtcgtc gcggtcccgc tgaacatcct
     1261 gctctgcatc gcgctgctga ggcacgcgca gctcctgcgc ggggggcagc gctccgcgat
     1321 gcgcgatctg gaggccgtct tcgcctaacg ggagccgtca tcccggcgga ggccgggact
     1381 tcggacaaga agtcccggcg gtctcttcga ggtcccggct ttcgccggga tgacggagcc
     1441 gctagtctcc ccttatgcca gacttcaccc aggccatcgt cctcatcacc ggcgccgcct
     1501 ccggaatcgg cgcggccacc gccacccggc tggccgaggc cggcgcccgc aagctcatcc
     1561 tgatggaccg ggacgaggac aagctccgcg acttcgcctt caccttgcct tgcgagcgcc
     1621 agatccggat cggcgacgtc gccgacgagc gcctgtggga gcgcgaggac ctgaccggcc
     1681 tcacccacgc cgtggtcaat gccggcatcg gggccggcgg ccccatcgcc gacaccagct
     1741 tcgccgattg gaggcaggtc atgtcgatca acctcgacgg cgccttcctc acgcttcagg
     1801 cggcgatgaa ggcgatgcgc gacacgggcg gctccatcgt cctcaccgct tcggtggcag
     1861 gcgtgaag
//
LOCUS       DASWNU010000161         4634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_394698, whole genome shotgun sequence.
ACCESSION   DASWNU010000161 DASWNU010000000
VERSION     DASWNU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394698"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /gene="clpB"
                     /locus_tag="VGB57_03180"
     CDS             <1..2099
                     /gene="clpB"
                     /locus_tag="VGB57_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY0130382.1"
                     /translation="ARDLTQAARDGKLDPVIGRDEEIRRTVQILARRTKNNPVLIGEP
                     GVGKTAIAEGLALRIANGDVPDSLKDRRLMALDMGSLIAGAKYRGEFEERLKGVLDEV
                     RQGEGDIILFIDEMHTLIGAGKSEGAMDASNLLKPALARGELHCIGATTLDEYKKHVE
                     KDPALQRRFQPVFIGEPTVEDTISILRGLKEKYELHHGVRITDGALVSAATLSNRYIT
                     DRFLPDKAIDLMDEAASRLRMEVESKPEEIENLDRRIIQLKIEREALKKEKDRASQDR
                     LKALEEELANLEQQSAELTQRWQGEKEKLAGEAKIKEELDRARLELEQAQRAGDLAKA
                     GELQYGRIPALEKQLAEVEAASATAMLREEVTSEDIASVVARWTGIPMERMLEGERQK
                     LINMEAEIGRRVIGQEEAVRAVAKAIRRSRAGLQDPNRPMGSFLFLGPTGVGKTELTR
                     SLALFLFDDPQAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGVLTEAVRRRPYQVV
                     LFDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFTNTIIILTSNLGSQFLTNLTEGQ
                     SVDEVEPQVMEVVRGHFRPEFLNRLDEIILFHRLAAEHMAPIVDIQVDRVRQLLADRK
                     IGLELSDGARAWLGRVGYDPVYGARPLKRAVQRYLQDPLADAILRGDVPDGSTVRVDE
                     GDGALTLDTGGQKAAA"
     gene            complement(2196..2732)
                     /locus_tag="VGB57_03185"
     CDS             complement(2196..2732)
                     /locus_tag="VGB57_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0130383.1"
                     /translation="MAEAPASVKARNAQSWDVLFSEYPQRALAAGQQGLVGYKVKLDR
                     EGYATECEVTKPSGHAVLDEETCRLILNRATFKGVRDSNGRRVATVTEGTVNWVLPSQ
                     AKTYSGTPAAAPAGLRIVDAATPASEKRICKRNLKTGTLATYERICATQAEWARHRNR
                     NRDEWGELQGAKGSTSGK"
     gene            complement(2837..3817)
                     /locus_tag="VGB57_03190"
     CDS             complement(2837..3817)
                     /locus_tag="VGB57_03190"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0130384.1"
                     /translation="MPKIQLPEPGRPHVLIEASGEAGILDLDPPRIVDLYKTHGALLL
                     RGFGADVGQFNAFARQFCSTSVFNESPGRQPLDPANNIHTVDGGTGPFSLHPELSREP
                     WKPDAAFFACLTAPSRGGATTICDGVELVRALPEEVRRGLEGRRLLYVKPTWPALLEY
                     WLGTPDPSDEELASPPASCPYRFRRYGDEVVRFFTRPALHRPMFIDAPAFGNFLLFAR
                     FNNKRPDHPLLDDGRQVPEAWLQAIKATGDRLMVEVAWRPGDVLMLDNTRFMHGRTAI
                     LDAGERLIATFFGYLSFAVPDPEEPPDAPWRRADFLPPIPPDQNRASPAR"
     gene            3869..>4634
                     /locus_tag="VGB57_03195"
     CDS             3869..>4634
                     /locus_tag="VGB57_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130385.1"
                     /translation="MSLTLDDLFESPDHYLQSFEGDAAIFVPMDRPAYHRSIFLDHRI
                     SPAANRSMRLPLSALSGEARPPAKTGWIFHVAHCGSTLLARALDDPGLNLVLREPLAL
                     RQLAFDRDPKRLPLTAAMLSKRYREDLPTIVKANVPVNFLLPELVALDRQAPAIFLYS
                     SLRDYLLAILRSENHRGWLHRVTAQLAAYLGDLSNLSDAERCAALWLAQMRAFGQAIS
                     LMPNARALDAEAFFAAPGQTLHAAAALLAVPMSESAI"
BASE COUNT          778 a         1537 c         1591 g          728 t
ORIGIN      
        1 tcgcgcgcga cctcacccag gccgcgcgcg acggcaagct cgatccggtc atcggccgcg
       61 acgaggagat ccggcgcacc gtccagatcc tcgcccgccg gaccaagaac aatccggtgc
      121 tgatcggcga gcccggagtc ggcaagaccg cgatcgcgga agggctggcg cttcgaatcg
      181 ccaatggcga cgttcccgac agcctcaagg accggcggct gatggcgctc gacatgggga
      241 gcctgatcgc cggcgcgaaa tatcgcggcg agttcgagga acggctgaag ggcgttctcg
      301 acgaagtgcg ccagggcgag ggcgacatca tcctgttcat cgacgagatg cacacgctga
      361 tcggcgccgg gaagagcgaa ggggcgatgg acgcctcgaa cctgctgaag cccgcgctcg
      421 cccgcggcga gcttcactgc atcggagcga ccacgctcga cgaatataag aagcatgtcg
      481 agaaggaccc ggcgctccag cggcgcttcc agccggtctt catcggcgag cccacggtcg
      541 aggacacgat ctcgatcctt cgcggcctga aggagaaata cgagctccac cacggcgtcc
      601 ggatcaccga cggcgccctg gtctcggccg cgaccctttc caaccgctac atcaccgacc
      661 gcttcctgcc cgacaaggcg atcgacctga tggacgaggc cgcctcgcgg ctgaggatgg
      721 aggtggagtc gaagccggag gagatcgaga atctcgaccg gcggatcatc cagctcaaga
      781 tcgagcgcga ggcgctgaag aaggagaagg accgagcttc gcaggaccgg ctgaaggcgc
      841 tcgaggagga gctcgccaat ctggagcagc agtcggccga gctcacccag cgctggcagg
      901 gcgagaagga gaagctggcc ggcgaggcga agatcaagga ggagctggac cgcgcccggc
      961 tcgagctcga gcaggcgcag cgcgccggcg acctcgccaa ggcgggcgag ctccaatacg
     1021 ggcggatccc ggcgctcgag aagcagctcg ccgaggtcga ggcggccagc gcgacggcga
     1081 tgctccgcga ggaagtgacc agcgaggaca tcgcctcggt ggtcgcccgc tggaccggaa
     1141 tcccgatgga gcggatgctg gagggcgagc gccagaagct gatcaacatg gaagccgaga
     1201 tcggccgccg ggtgatcggc caggaggaag cggtgcgcgc cgtcgccaag gcgatccgcc
     1261 gctcgcgcgc cggccttcag gacccgaacc ggccgatggg gagcttcctg ttcctcgggc
     1321 cgaccggggt cggcaagacc gagctcacca ggtcgctcgc tctgttcctg ttcgacgatc
     1381 cccaggcgat ggtccgaatc gacatgtccg agttcatgga gaagcacagc gtcgcccggc
     1441 tgatcggcgc tcctcccggc tatgtgggct atgaggaagg cggggtgctg accgaggcgg
     1501 tccggcgccg gccctatcag gtcgtgctgt tcgacgaggt cgagaaggcg cattcggacg
     1561 tcttcaacat cctcctccag gtgctcgacg acggtcggct gaccgacggc cagggacgca
     1621 ccgtcgactt caccaacacg ataatcatcc tgacctcgaa cctgggcagc cagttcctga
     1681 ccaatctgac cgaagggcag agcgtcgacg aggtcgagcc gcaggtgatg gaggtggtgc
     1741 ggggccattt ccggccggaa ttcctcaacc gcctggacga gatcatcctg ttccaccgcc
     1801 tcgcggccga gcatatggcg ccgatcgtcg acatccaggt cgaccgggtc cggcagctcc
     1861 tggccgaccg caagatcggt ctcgagctca gcgacggcgc ccgcgcctgg ctgggccggg
     1921 tcggctacga tccggtctac ggggcgcggc cgctcaagcg ggcggtgcag agatatctgc
     1981 aggacccgct ggcggacgcg attcttcggg gcgacgtgcc tgacggttcg accgtccggg
     2041 tcgatgaagg cgacggcgct ctgacgctcg acaccggtgg gcagaaggcg gcggcgtagc
     2101 gcttcgccat cccggcgaag gcaggaatcc cagggggtga aaagctcggc gcctatcgcg
     2161 ctgggatgcc ggcctccgcc gggatgacga atgcgctatt tgccgctcgt gctgcccttc
     2221 gcgccctgca gctctcccca ttcgtcgcgg ttgcggttgc ggtggcgggc ccactccgcc
     2281 tgagtcgcgc agattctctc gtaggtggcg agcgtgccag tcttcaggtt gcgcttgcag
     2341 atccttttct cggaagccgg cgtagcggcg tcgacgatcc ggagccccgc aggcgccgcc
     2401 gcgggcgtac ccgagtaagt cttggcctgg ctcgggagca cccagttgac ggtgccctcc
     2461 gtgacggtgg ccacccggcg accgttcgaa tccctcacgc ccttgaaggt ggcgcggttg
     2521 aggatcagcc ggcacgtctc ttcatccaga acggcatggc cgctgggctt ggtcacttcg
     2581 cactcggtcg cataaccctc gcggtcgagc ttcaccttgt agccgaccag gccctgctgg
     2641 cccgcggcga gcgcgcgctg cggatattcg ctgaagagga cgtcccagct ctgggcattg
     2701 cgcgctttca cgctggcggg ggcttcagcc aaagccggcg ccgctgctgc ggccagaagc
     2761 gctgcgaata agaccattcg agccatatat atgtccctca cattcccagc gaacttaccc
     2821 gaaatcgacc gcgatttcaa cgtgccgggc ttgctcggtt ctggtcgggc ggaatcggcg
     2881 gcaggaagtc cgctcggcgc cagggcgcgt caggcggttc ctccgggtcc ggaacggcga
     2941 agctcaagta gccgaagaag gtcgcgatca gcctttcgcc ggcgtcgagg atggcggtcc
     3001 ggccgtgcat gaagcgggta ttgtcgagca tcagcacgtc tcccggccgc caggcgacct
     3061 cgaccatcag ccggtcgcca gtcgccttga tcgcctggag ccaggcctcc ggcacctgcc
     3121 ggccgtcgtc gagcagcgga tgatccggcc gcttgttgtt gaagcgggcg aagagcagga
     3181 aattgccgaa ggccggagcg tcgatgaaca tggggcgatg gagggccggg cgggtgaaga
     3241 acctgaccac ttcgtcccca tagcggcgga atcgataggg gcaggaggcg ggcgggctcg
     3301 cgagctcctc gtcgctcggg tcaggcgttc ccagccaata ttcgagcaag gcgggccagg
     3361 tcggcttcac gtagagcagc cgccgtccct ccagcccgcg cctgacctcc tcgggcagcg
     3421 cccggaccag ctcgaccccg tcgcagatcg tcgtcgctcc gccccggctc ggagccgtga
     3481 ggcaggcgaa gaaggctgcg tccggcttcc acggctcgcg cgacagctcg ggatgaagcg
     3541 aaaagggccc ggtgccgccg tcgaccgtat ggatgttgtt ggcgggatcg agcggctgac
     3601 ggcccgggct ttcgttgaac acggaggtcg agcagaactg gcgcgcgaag gcgttgaact
     3661 gcccgacgtc ggcgccgaag ccgcgcagaa gcagcgcgcc atgggtcttg tacaaatcga
     3721 cgatccgggg tggatcgagg tcgaggattc cggcctcccc cgacgcttcg atcagcacgt
     3781 ggggacggcc cggttctggc agctggatct tcggcatggc ggggccgggg gatagccgat
     3841 gtcggggttg cgggctatag gccggacgat gtccctgacg ctcgacgacc tgttcgaatc
     3901 tccggatcat tatctccagt ccttcgaagg cgacgcggcg atcttcgtgc cgatggaccg
     3961 ccccgcctat caccgctcga tcttcctcga tcaccgaatc tcgccggcgg cgaaccggtc
     4021 gatgcggctg ccgctgtcgg cgctgagcgg cgaggcgcgg ccgccggcga agaccgggtg
     4081 gatcttccat gtcgcgcact gcggatcgac cttgctggcc cgggcgctcg acgatccagg
     4141 cctcaacctg gtgctgcgcg agccgctcgc gcttcgccag ctggccttcg atcgagaccc
     4201 gaagcggctg cctctgaccg cggcgatgct gtccaaacgc tatcgcgagg acctgccgac
     4261 gatcgtcaag gccaatgtcc cggtgaactt cctgctcccc gagctcgtgg cgctcgatcg
     4321 gcaggcgccc gccatcttcc tctacagctc gctcagggac tatctgctcg ccatcctgcg
     4381 cagcgaaaat catcgcggct ggctgcatcg ggtcaccgcc cagctcgccg cctatctggg
     4441 cgatctttcg aacctgtccg acgccgagcg ctgcgcggcc ttgtggctgg cgcagatgcg
     4501 ggcgttcggg caagcgatct cgctcatgcc caacgcccgg gcgctcgacg ccgaagcttt
     4561 cttcgccgcc cctggccaga cccttcacgc cgccgcggcc ctcctcgccg tgccgatgag
     4621 cgagtcggcg atcg
//
LOCUS       DASWNU010000162         4891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_6489,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000162 DASWNU010000000
VERSION     DASWNU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6489"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VGB57_03200"
     CDS             complement(<1..1750)
                     /locus_tag="VGB57_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130386.1"
                     /translation="MTWPSAILSALYGFIASNRQTGGAAGLNAFTAWLNGPGNGLIRA
                     YVPATPGQGHQSATANILRRLFMPPPNGLGYAGRVNVYLDRAGSQETFVQLDRRFRAL
                     LPELVNMVPPYRFWGGTLNTIDLKQPPKDTVKFGLSGGSDDNTFDYAKKMNVDYFLRL
                     QPYRWGITIFGSFAFMPQEIRFLDKTRGTGGTLNLTSQKALGGATLAERAYKQRMATI
                     DWATFPQADQRAQIIKWLIAQAAAGGFDLMPVYGVNAPQECAMGKPAQDRVVEILAAL
                     RAAQGLAAAPRPAVALNLSVFEPSAVNSFAQIGGILNGGRTNDEALGVVAANAQHETA
                     RKAYLTALNPDGRHYEFFIYEPGNANNLTNLQAAVAANLNGHGDRVLWVQLGRVEQPP
                     YVLPPRLFQEAFAGGTAPPIPPTNFLPPVFEGQNTANFMLNTGRPYFHVNQPDRFDIQ
                     YPTTLLGIHNDDAEIPRDLQDAASQLNKRLETWAVVPARNPAAVMGAMIQELRAENGL
                     GPLNGYFLDIGEFYESDLNDKLGLVLSFMSSVVGAPPAPARSLADGEGGALAAIYESF
                     STAPDPVGFAAMLDPAG"
     gene            complement(1747..>4891)
                     /locus_tag="VGB57_03205"
     CDS             complement(1747..>4891)
                     /locus_tag="VGB57_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130387.1"
                     /translation="SLAVAIAGDSLVFTVPAGFQNGLYFSTAIGTVGAFGQAKTATLP
                     LSGNDAGALLFSSRFQRATLAGTLNWGMQLLYTDPDGAPGNFRSQLYPFAESVLDPSD
                     TIGFAVRLDPSDVANRQVTERTLFTFEEAADGKPATLASGYRTAWGDRIDLVPVTAAA
                     DGEIPAGLVLTVGNPGTPGGAQFALAPRGDFVLHVPPPKHQVPQPLLCGLTSTETITF
                     TPGIDRLRFLTGRCAFASGYPPAQASALGPPVDTSVAPMTDRFLTSWATVIAGPAKSD
                     LAAQKPGIHYSAQPRGQPLFGLDEVVAPAAPGTLGPVDPGYILPHAPDFAVPLMPYGL
                     ALPTGLPTGFDSNAVAAVEARIVAPRRRTAIGGPDSTITSIAPHHRRAALLADGATSI
                     QATTPMGLVATVDSEGRWDGVGLALNAEAGVRLGFCKPPPALQQALQSSELFLVIANA
                     GQLGTLTGWNPAGSMAPPADPPSFFNAMNVADWRIEARVGSNAGYGDYSNVMIVKGCR
                     GSVTDLVTRTSAWTQAGTFAAPSYPAAPAAPVPGDVVALSTWLNDYIAAARARLSNAE
                     DRRYFQNFIDIVDSTTWTGILVLKASLTQLPADLGGLLATIDPAYAFAHHFGVSVSPV
                     DAATVSVNRTSAMFQLVDYVEPGYVDAAATTAPMAPQGTGPYDFQLLTMKALFENSAV
                     AAFSSKAQLTLGAWFGDSVGSMKGAEANSYNTIFLIGSYQSQHGRSGYVLDCETSNKF
                     LLDSDAIAGIEVVKAQYVSIDDQDSRFDIWGFIDFALIGGTGTPFDIFSFGGSAGVDD
                     PPRTGLSFSGLALGMSVDSAQNKTFAPDHARMAFDVARSTPRPGSLYRDFALRPPAIV
                     IGAADAGPLDRGYLTVGSTAPTLTGVGQGKAWFGLSFGLDMGSIGALAGGAGLTATML
                     CAWAPKASGGGAASDGSAFPVQLGLALPGVNPTAKLMSLEGVLRLGIGELSITHAPVA
                     TPDGTASTEPYWVLSLDDIGLKFLGFYKIPPSGSISFTCFGNPGAAAGPSALGWYAVY
                     NQTPPAPGGIGSGSGGR"
BASE COUNT          818 a         1541 c         1744 g          788 t
ORIGIN      
        1 gccccgccgg gtcgagcatt gcggcgaagc cgaccgggtc gggggcggtc gagaagcttt
       61 cgtagatcgc cgccagcgcg ccgccttcgc catcggccag agaccgtgcc ggggccggtg
      121 gggctccgac cacggagctc atgaaggaga ggacaagacc gagcttgtca ttgagatcgc
      181 tttcgtagaa ctcaccgata tccagaaaat aaccgttgag cggcccgagc ccgttctcgg
      241 ccctgagctc ctggatcatc gcgcccatta cggccgcggg attcctggcc gggacgaccg
      301 cccaggtttc gagccgcttg ttcagctggc tcgcggcgtc ctgcaggtcg cgcggtatct
      361 ccgcgtcgtc gttgtggatg cccagcagcg tcgtcggata ttgaatgtcg aagcggtccg
      421 gctggttgac gtggaaatag ggtctccccg tgttgagcat gaaattcgcg gtgttctggc
      481 cttcgaagac cggggggagg aaattggtgg gcgggatggg cggagcggtc cccccggcga
      541 aagcttcctg gaaaagccgt ggcggcagga cgtaaggcgg ctgctcgacc cggcccaact
      601 ggacccacag cacgcggtcg ccgtgcccgt tcagattagc ggcgaccgcg gcctgcaggt
      661 tcgtcaggtt gtttgcattg ccgggttcgt agatgaaaaa ttcataatgc cggccgtccg
      721 ggttcaaagc ggtgagatag gccttgcgag cggtctcgtg ctgcgcattc gcagcgacaa
      781 cgccgagcgc ttcgtcgttc gtccggccgc cgttgaggat gccccctatc tgggcaaagc
      841 tgttgaccgc ggagggttcg aagacactca ggttgagcgc cacggcgggc cgcggcgcgg
      901 ccgccaagcc ttgggcggcc cgcagggcgg cgaggatctc gacgaccctg tcctgtgcgg
      961 gcttgcccat tgcgcattcc tggggagcgt tcactccgta aaccggcatc aggtcgaagc
     1021 caccggcggc ggcttgcgcg atcagccatt tgatgatctg cgcccgctgg tcggcttgtg
     1081 ggaatgtggc ccagtcgatc gtcgccattc tctgcttgta ggctcgctcg gcgagcgttg
     1141 ccccgcccag cgccttctgg ctcgtcagat tgagggtgcc gcccgtgccc cgcgtcttgt
     1201 cgaggaagcg aatctcctgg ggcatgaagg cgaacgatcc aaagatcgtg attccccagc
     1261 gataaggctg gagccggaga aaatagtcga cgttcatctt cttcgcgtaa tcgaaggtgt
     1321 tgtcgtcgga ccctcccgag aggccgaact tcaccgtgtc cttggggggc tgcttgaggt
     1381 cgatcgtatt gagagtcccg ccccagaaac ggtagggcgg aaccatattg acaagctcgg
     1441 gcagcaacgc ccggaagcga cggtccagct ggacgaacgt ttcctggctt cccgcgcgat
     1501 cgagatagac gttgacgcgg cccgcataac ccaggccgtt cggaggcggc atgaacagtc
     1561 gccgaaggat gttcgccgtc gccgactggt ggccctggcc gggggttgcc ggcacgtagg
     1621 cgcggatcag gccattgccg ggcccgttca gccaggccgt gaaggcgttg aggcctgctg
     1681 cccctcccgt ctgccggttg gacgcgataa agccgtaaag cgcggacagg atggcgctcg
     1741 gccaggtcat cgaccgcccg aacccgatcc gattccgccc ggcgcgggcg gagtctggtt
     1801 gtagacggcg taccagccga gcgcggacgg cccggccgcg gcgccgggat tgccgaagca
     1861 ggtgaagctg atcgagccgc tcggcgggat cttgtagaag ccgaggaact tgaggccgat
     1921 gtcgtcgagc gacagcaccc agtaaggctc cgtcgaagcg gtcccgtccg gcgtcgccac
     1981 cggagcgtgg gtgatgctga gctctccgat cccgagccgc aggaccccct cgagcgacat
     2041 cagcttggcc gtcgggttga cccccggcag cgcaaggccg agctgtaccg gaaaggcgct
     2101 tccatccgaa gccgcgccgc cgccggaagc cttcggcgcc caggcgcaga gcatcgtcgc
     2161 cgtgagcccg gcgccgccgg caagcgcgcc gatcgagccc atgtcgagcc cgaaggagag
     2221 gccgaaccag gccttgccct gaccgacgcc ggtcagagtc ggcgccgtcg atccgaccgt
     2281 cagatagccc cggtcgagcg ggccggcgtc ggcggcgccg atcacgatgg ccggcgggcg
     2341 cagcgcgaag tccctgtaga gcgagcccgg ccgcggcgtg ctccgggcaa cgtcgaaggc
     2401 catcctggcg tgatcgggcg cgaaggtctt gttctgcgcg gaatcgaccg acattccgag
     2461 cgccagcccg gagaagctga gcccggttcg ggggggatcg tcgactccgg cgctgccgcc
     2521 gaagctgaag atgtcgaacg gagtgccggt gccgccgatc agcgcgaagt cgatgaagcc
     2581 ccagatgtcg aaacgcgaat cctggtcgtc gatgctgaca tattgcgcct ttaccacctc
     2641 gatgcccgcg atcgcatcgc tgtcgaggag gaatttgttg ctggtctcgc aatcgagcac
     2701 gtagccgctg cggccgtgct ggctctggta ggagccgatc aggaagatcg tattgtagct
     2761 gttcgcttcc gcgcctttca tgctgccgac cgaatccccg aaccaggcgc cgagcgtcag
     2821 ctgggccttg ctcgaaaagg cggcgacggc gctgttctcg aacagcgcct tcatcgtcag
     2881 aagctggaaa tcgtagggtc cggtgccttg cggcgccatc ggcgcggtcg tggccgcggc
     2941 atcgacatag ccgggctcga catagtcgac gagctggaac atcgcgctcg tccggttgac
     3001 cgaaacggtg gcggcgtcga ccgggcttac cgacaccccg aaatgatggg cgaaggcata
     3061 ggccgggtcg atcgtcgcaa gcaggccgcc aaggtccgcg ggcaattgcg tcagcgacgc
     3121 cttgaggacg agaattccgg tccaggtggt cgagtcgacg atgtcgatga aattctggaa
     3181 atagcgccgg tcttcggcgt tgctcaggcg cgccctcgcc gccgcgatat aatcgttcag
     3241 ccaggtcgac aacgcgacga catctccggg gacgggagcg gcgggggcgg ccggatagga
     3301 aggggccgcg aaagtcccgg cctgcgtcca ggcgctggtc cgggtgacga ggtcggtgac
     3361 gctgcccctg cagcccttga cgatcatcac gttgctgtag tcgccatagc ccgcgttcga
     3421 gccgaccctg gcttcgatcc gccagtcggc gacgttcatc gcgttgaaga agctcggcgg
     3481 atcggccggc ggggccatcg agccggcggg attccagccg gtcagcgttc ccagctgacc
     3541 ggcattggcg atgacgagga acagctcgga gctctgcagc gcctgctgaa gcgccggcgg
     3601 cggcttgcaa aagcccagcc gcaccccggc ctcggcgttg agggcgaggc cgacgccgtc
     3661 ccagcggcct tcggaatcga ccgtcgcgac gaggcccatc ggcgtggtcg cctgtatcga
     3721 ggtcgcgccg tcggcgagca acgcggcgcg ccggtggtgc ggggcgatcg aggttatcgt
     3781 cgaatcgggg ccgccgatcg ccgtacggcg ccggggagcg acaatccgcg cttccacggc
     3841 ggcgaccgca ttcgagtcga agccggtcgg cagcccggtc gggagggcca ggccgtaagg
     3901 catgagcggc accgcaaaat ccggggcgtg gggcaagata tagccgggat cgaccggtcc
     3961 gagcgttccg ggcgcggcgg gcgccaccac ctcgtcgagg ccgaacagag gctggcccct
     4021 gggctgggcg gaataatgga ttccgggttt ctgcgcggct aaatcactct tggcgggacc
     4081 ggcgatcacg gtcgcccagc tggtcaggaa ccgatcggtc atcggcgcca cgctggtatc
     4141 gaccggcggg cccagcgccg acgcctgtgc cggcggatag cccgaggcga aagcgcaccg
     4201 gccggtcagg aagcgcagcc tgtcgatgcc gggggtgaag gtgatggttt cggtcgacgt
     4261 aagcccgcag agcagaggct ggggtacctg atgcttcggc ggcggtacat ggaggacgaa
     4321 gtcgccgcgc ggagccaagg cgaactgagc ccctcccggc gtacccggat tgccgacggt
     4381 gaggacgagg cccgccggga tctcgccgtc cgctgcggcc gtaaccggga cgagatcgat
     4441 ccggtcgccc caggcggtcc ggtagccgct ggccagcgtc gccggcttgc cgtcggcggc
     4501 ctcctcgaag gtgaagagcg ttcgctccgt cacctggcgg ttggcgacgt ccgaggggtc
     4561 gagccggacc gcgaagccga tcgtgtcgga cgggtcgagc acgctttcgg cgaacggata
     4621 aagctgcgag cggaaattgc cgggcgcacc gtcgggatcg gtatagagca gctgcatgcc
     4681 ccagttcagc gtgccggcga gggtcgcgcg ctggaaccgg ctcgagaaga gcagcgcccc
     4741 ggcatcgttc ccactcaggg gcaaagtcgc ggtcttggcc tgtccgaagg cgccgacggt
     4801 ccctatcgcg gtcgagaagt agaggccgtt ctggaatccg gccggaaccg tgaagaccag
     4861 ggagtcgccg gcgatcgcga cggcgaggct g
//
LOCUS       DASWNU010000163         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_368724, whole genome shotgun sequence.
ACCESSION   DASWNU010000163 DASWNU010000000
VERSION     DASWNU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368724"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VGB57_03210"
     CDS             <1..1056
                     /locus_tag="VGB57_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0130388.1"
                     /translation="RFQGDLTLARLGVLPNRLDRLKGKGFRLDLSGVGRMDTIGAWIV
                     HRTARDRGGEIVGADEGAATLLRQVEKADQPVKVRPDPPAPFERVLTQLGDATVEAGR
                     TFVGLLGFFGAMLLSIGYVIRQPRRFRLNAVVQRFEVVGVHAFGIIGLMSFLVGIVIA
                     QQGAVQLRQFGAEVFTVNLIGRSSIKELGVLMTAIMVAGRSGSSFAAQLGSMKLAEEV
                     DAMRTIGVSPMEALVLPRVFATVLMMPLLGFYAAIVAIIGGAVFCWISLDIPPVTFIQ
                     RTREVVPLTDLWQLLIKAPVFGLIISLAGCFQGMQVKSDAEEVGLRTTTAVVQAIFLV
                     IVLDAFFAVFFTGIGWL"
     gene            1053..1877
                     /locus_tag="VGB57_03215"
     CDS             1053..1877
                     /locus_tag="VGB57_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0130389.1"
                     /translation="MSEPGETVIEVRGLRNSFGEQLIHDGLDLEVKRGEILGVVGGSG
                     TGKSVMMRSIIGLQRPDAGEVRVFGEPMLDRDESELQGIRKRWGILFQGAALFSTLTV
                     AENVQVPLREFYPRLSEKLLDEIAAYKIVMVGLSPDAGPKFPAELSGGMKKRAGIARA
                     LALDPDLLFLDEPTAGLDPIGAAAFDQLIVDLRERLGLTVFLITHDLDTLYTICDRVA
                     VLADQKVIAVGTIPELLDLDHEWIQEYFRGPRGRAAEEASEEAEARTAEANGGTER"
     gene            1877..>2846
                     /locus_tag="VGB57_03220"
     CDS             1877..>2846
                     /locus_tag="VGB57_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0130390.1"
                     /translation="METRSNHILVGAVVLGMLVAVVLFVVWLSQAGGDQDKKYDILFS
                     QAVEGLAKGSAVTFSGVPVGQVESINLMPQTPQFVRIRISVDDETPVIVGTTATMKAT
                     FTGIASIQLDPPERDPRRPAGEGRPIVCPEVNADAQCPFGVPLIPTKPGALGQLLNSA
                     PELLERVSALTARLTELLGDRNQNSIAAILENVQVISRNLAERSDEIAATLAEARVAI
                     RQTGVAAQEMGQLADTADRSLSQDVRPMLADLRQTIRAAETSMGTLDATLSDARPGLQ
                     AFSKQTIPEVGQLVRDLREMSDSLNAVAQRLNTQGAGGIIGGSKLPD"
BASE COUNT          450 a         1002 c          925 g          469 t
ORIGIN      
        1 cgtttccagg gtgacctgac gctggcgcgg ctcggcgttc ttcccaatcg cctcgaccgc
       61 ctcaagggaa aggggtttcg tctcgacctg tccggcgtcg gccggatgga cacgatcggc
      121 gcctggatcg tccatcgcac ggcgcgcgac cgcggcggcg agatcgtcgg cgccgacgag
      181 ggcgccgcta ccctgcttcg gcaggtggag aaggccgacc agccggtcaa ggtccgcccc
      241 gatccgcccg cgcccttcga acgggtgctc acccagctcg gcgacgcgac ggtcgaggcc
      301 ggccggacct tcgtcggact gctcggcttc ttcggggcga tgctgctctc gatcggctat
      361 gtcatccgcc agccgcgccg cttccggctc aacgccgtcg tccagcgctt cgaggtggtc
      421 ggggtccacg ccttcgggat catcggcctg atgagcttcc tggtcgggat cgtcatcgcc
      481 cagcagggcg cggtccagct ccgccagttc ggagccgagg tgttcaccgt caacctcatc
      541 ggccgaagct cgatcaagga gctgggcgtg ctgatgaccg cgatcatggt cgcgggccgc
      601 tccggctcct ccttcgccgc ccaattgggc tcgatgaagc tggccgagga agttgacgcg
      661 atgcggacga tcggagtgtc cccgatggag gcgctcgtcc tccccagggt attcgccacc
      721 gtcctgatga tgcctctgct cggcttctac gccgccatcg tcgcgatcat cggcggcgcc
      781 gtcttctgct ggatctcgct cgacataccg ccggtcacct tcatccagcg cacccgcgag
      841 gtggttccgc tgaccgacct ttggcaattg ctgatcaagg cgcccgtctt cggcctgatc
      901 atctcgctgg ccggctgctt ccagggcatg caggtcaagt cggacgccga ggaggtgggc
      961 ctcaggacca ccaccgcggt cgtccaggcg atcttcctcg tcatcgtcct cgacgccttc
     1021 ttcgccgtct tcttcaccgg gattgggtgg ctgtgagcga gccgggcgaa accgtcatcg
     1081 aggtccgcgg cctccgcaac agcttcggcg agcagctcat ccacgacggg ctcgacctcg
     1141 aggtgaagcg cggcgagatc ctcggagtgg tcggcggctc gggcaccggc aagtcggtga
     1201 tgatgcgctc gatcatcggg ctgcagcggc ccgatgcggg cgaggtgcgc gtgttcggcg
     1261 agccgatgct cgaccgcgac gagtccgagc tccagggcat ccgcaagcgc tggggaatcc
     1321 ttttccaggg cgcagccttg ttctcgacgc tgaccgtcgc cgagaacgtc caggtgccgc
     1381 ttcgcgaatt ctatccgcgc ctgtcggaga agctgctcga cgagatcgcc gcctacaaga
     1441 tcgtcatggt cggcctctcc cccgacgccg gacccaaatt cccggccgag ctgtcgggcg
     1501 ggatgaagaa gcgcgccgga atcgcccgcg ccctcgccct cgatcccgac ctgctcttcc
     1561 tcgacgagcc caccgccggc ctcgatccga tcggcgccgc cgccttcgac cagctgatcg
     1621 tcgatcttcg cgagcggctc ggcctgaccg tctttttgat cacgcacgat ctcgacacgc
     1681 tctacaccat ctgcgaccgt gtcgccgtgc tcgccgacca gaaggtgatc gcggtgggga
     1741 cgatcccgga attattggat ttggaccacg aatggataca ggagtatttc aggggtccgc
     1801 gcggccgggc ggccgaagag gccagcgagg aagccgaggc acggacggcc gaagcgaacg
     1861 gggggactga aagataatgg aaacccgctc gaaccatatc ctggtaggcg cagtcgtcct
     1921 cggaatgctg gtggccgtcg tcctgttcgt cgtctggctc agccaggcgg gcggcgacca
     1981 ggacaagaaa tacgacatcc ttttcagcca ggcggtcgaa ggcctggcca agggcagcgc
     2041 cgtcaccttc tccggcgtcc cggtcggcca ggtcgaatcg atcaacctga tgccgcagac
     2101 gccgcaattc gtccggatcc gaatctcggt cgacgacgag actccggtca tcgtcggaac
     2161 caccgcgacg atgaaggcga ccttcaccgg aatcgcctcg atccagctcg acccgcccga
     2221 gcgcgacccg cgccggccgg ccggcgaggg ccgtccgatc gtctgccccg aggtcaatgc
     2281 agacgcccaa tgcccgttcg gggtgccgct catcccgacg aagcccggag cgctgggcca
     2341 gctgctcaac agcgcgcccg agctgctcga gcgggtctcg gcgctgaccg cgcggctcac
     2401 cgagctgctc ggcgaccgca accagaattc gatcgccgcg atcctcgaga acgtccaggt
     2461 gatcagccgc aacctcgccg agcgctcgga cgagatcgcg gccacgctcg ccgaggctcg
     2521 ggtcgcaatc cgccagaccg gagtggcggc ccaggaaatg ggccagctcg ccgacacggc
     2581 ggaccggtcc ctgtcccagg acgtgcgtcc gatgctcgcg gacctgaggc agacgatccg
     2641 ggcggccgag accagcatgg gcactctcga cgccacgctg agcgacgccc ggcccggcct
     2701 gcaggccttc tccaagcaga ccatccccga ggtcggccag ctggtccgcg acctgaggga
     2761 gatgtccgat tcgctgaacg ccgtcgccca gcgcctcaac acccagggcg cgggcgggat
     2821 catcggcggc tccaagctgc ccgact
//
LOCUS       DASWNU010000164         6652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_239834, whole genome shotgun sequence.
ACCESSION   DASWNU010000164 DASWNU010000000
VERSION     DASWNU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239834"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1338
                     /locus_tag="VGB57_03225"
     CDS             97..1338
                     /locus_tag="VGB57_03225"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY0130391.1"
                     /translation="MARIVMKFGGTSMAGIERIRHVAQRVKNEAEAGNQVAVVVSAMA
                     GETDRLIQFCREASSLYDPREYDVVVASGEQVTSGLLAITLQGMGVNARSYMGWQLPI
                     RTSGHASALIDDIDASVLERVFGEGGIAVIPGFQGVAADGSLATLGRGGSDTSAVALA
                     AAVNADRCDIYTDVDGVYTTDPRIVPRARKLPAVTYEEMLELASVGAKVLQTRSVGLA
                     MRENIPVRVLSSFDDRPGTMVVGDEAIEEYNMERQLITGIAYDKNEARVTLTGVPDRP
                     GAVAGVFAPLAAGSINVDMIVQSVPRAGQKSDLTFTVPTASLAQSRDILDKAREAVGF
                     DEILTDTNVVKVSAVGVGMRSNAGIAATMFQTLADRGINILAITTSEIKVSVLIAEEY
                     TELAVRVLHTAYGLDDLGDEE"
     gene            1451..2476
                     /locus_tag="VGB57_03230"
     CDS             1451..2476
                     /locus_tag="VGB57_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0130392.1"
                     /translation="MSDTLGHERLQRLMKRGTDFLGCEIAILGGAMSWISERNLVSAL
                     SNAGAFGVIACGAMTPDLLDTEIAETKKRTNKPFGVNLITMHPQLSELIDVCANHEVS
                     HVVLAGGLPPPGSIDRIKGNGAKLICFAPALALAKKLARSGVDALVIEGMEAGGHIGP
                     VSTSVLAQEILPPLAADMPIFVAGGIGHGGLMAGYLEMGAVGCQLGTRFVCATECIAH
                     PNFKKAFIRASARDAITSVQIDPRLPVIPVRALKNREMESFAAKQREVAQRLDSGEVE
                     MMEAQLQIEHYWAGALRRAVIEGDVETGSVMAGQSVGMVKEEAPVAEILKQLVDEAAQ
                     ALERRSA"
     gene            complement(2473..2874)
                     /locus_tag="VGB57_03235"
     CDS             complement(2473..2874)
                     /locus_tag="VGB57_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0130393.1"
                     /translation="MLASLDTNVVIYLADRDREKAARSRDLIGEGGWISAQVLNEVAA
                     VARRKIRLAWPDVHELLELVRSFFIVEPLTLETHEQTLFLAERYKLDVYDSAVVASAL
                     LAGCSTLWSEDMHHGLIVEGTLTIRNPYRRD"
     gene            complement(2864..3094)
                     /locus_tag="VGB57_03240"
     CDS             complement(2864..3094)
                     /locus_tag="VGB57_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015463213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0130394.1"
                     /translation="MKVAKWGNSLAIRIPKDVAEALELKEGDEIELAPAEKPVIGIAR
                     RMSREEALERLKRFRGLLPAGYKFDRSDAYAR"
     gene            complement(3220..3348)
                     /locus_tag="VGB57_03245"
     CDS             complement(3220..3348)
                     /locus_tag="VGB57_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130395.1"
                     /translation="MGRQPIGAAMRDGIPDYKAWVRIPITFYNCRKDEPACIDSLE"
     gene            3543..5816
                     /gene="ptsP"
                     /locus_tag="VGB57_03250"
     CDS             3543..5816
                     /gene="ptsP"
                     /locus_tag="VGB57_03250"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY0130396.1"
                     /translation="MPAQSAASAREILTGLHDVMASRASAQAKLNKVVKIIAEAMASE
                     VCSIYLLRDGVLELFATLGLNQSAVHVTKLALGEGLVGTIAKDVSILNLAEAPSHPEF
                     AYRPETGEELFHSFAGVPIVRRERAIGVLAVQHAEARAYDDLEIEALQTVAMVLAELI
                     ANAGLADKAAAAGSTTRGDGPERLDGLKLVAGMARGQAVYHQPRILIEHTVAEDVEAE
                     RHRVYSAFRKMREQIDNMTNLAEFGTAGEHQEVLETYKMFAYDEGWSRRINEAIDSGL
                     TAEAAIERVRQRMQMRMREIDDSLLQDRMHDLDDLSNRLLRIVAGQLGTAAQMGLPHD
                     AVLIARNLGPAELLEYDRRKLKGVILEEGSLTAHVTIVARAMGVPMLGRAGNVRQTIG
                     EGDEVLLDGDAGVAFVRPTGAVEEAFKAKFLITKKRRAEFAALRDLPPVTTDGSRITV
                     MVNAGLRDDVAALEMMGADGIGLFRTEFQFLVSATLPSRESQQRLYKAVLDAAGDKPV
                     IFRTVDVGGDKALPYIDMGDGGIEENPAMGWRALRLALDRDGLMKAQARALLGAAAGR
                     ELNVMFPMVSEPWEYDEARALVEQQREWLAGHKKRLPTSIRYGAMLEVPALAETLDIL
                     LPKVDFLSIGTNDLTQFLFAADRAHPKLAERYDWLSPSILRFLRRIVREADAAGVPVG
                     VCGEMGGRTLEAMALIGIGLTRFSITPAAVGPIKAMIRSLDRAALLPVMEELLARPPA
                     DLRGALAQWAEGRGVKV"
     gene            6034..>6652
                     /locus_tag="VGB57_03255"
     CDS             6034..>6652
                     /locus_tag="VGB57_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130397.1"
                     /translation="MADADQDQLLGQSVGERLRIAREAKKLSLDDVARQTRIPVRHLE
                     HIEREEWDALPAITYSVGFARSYANTVDLDGAKIGAEVRQQLGGIRAGHTAAAPFYEP
                     TDPARVPPRSIAIAAAVIAVLLVIGYLVWRSGAVGEESPADVAITQADEPAAANQAAA
                     RPARPVVAAPPPSNGPVVLTAAEDVWLRIDQAGGGAPLYMGTLKGG"
BASE COUNT         1127 a         2177 c         2291 g         1057 t
ORIGIN      
        1 ttcgccgggg tgacgggaga ggtctcggcg agccccggct ttcgccgggg cgcaagcgca
       61 ttgcaccgcc gccaccgcca acctatcagc ccccgcatgg cccgcatcgt gatgaagttc
      121 ggcggcacct cgatggcggg catcgagcgc atccgtcatg tcgcgcagcg ggtgaagaac
      181 gaggcggagg cggggaacca ggtcgccgtc gtggtctcgg cgatggcggg ggagacggac
      241 cggctgatcc agttctgccg cgaggcgtcc agcctctacg atccgcgcga atatgacgtg
      301 gtggtggcga gcggggagca ggtgacgagc gggcttctcg ccatcaccct gcagggcatg
      361 ggggtcaacg cccgttccta tatgggctgg cagcttccga tccggacctc gggccacgcc
      421 tcggcgttga tcgacgacat cgacgcgagc gttctcgagc gcgtgttcgg ggaggggggc
      481 atcgccgtca ttcccggctt ccagggcgtc gccgccgacg gcagcctcgc cacattgggc
      541 cgcggcggct cggacacctc ggccgtggcg ctggcggcgg cggtgaacgc cgaccgctgc
      601 gacatctata ccgacgtaga cggagtctac acgaccgacc ccaggatcgt gccgagggcg
      661 cgcaagcttc ccgccgttac ctatgaggag atgcttgagc tggccagcgt cggcgccaag
      721 gtgctccaga cccgctcggt cgggctcgcc atgcgcgaga atatccccgt ccgggtcctc
      781 tcgtcgttcg acgaccggcc cggcaccatg gtcgtgggcg acgaagccat cgaggaatac
      841 aatatggaac ggcaactcat caccggcatc gcctatgaca agaacgaggc tcgggtgacc
      901 ctgaccggcg ttccggaccg ccccggagcg gtcgcggggg tcttcgctcc gctcgccgcc
      961 ggctcgatca acgtcgacat gatcgtccag tcggtgccgc gcgccgggca gaagagcgac
     1021 ctcaccttca ccgttcccac cgcctcgctc gcccaaagcc gcgacatcct cgacaaagcc
     1081 cgcgaggcgg tcggcttcga cgagatcctg accgacacca acgtcgtcaa ggtcagcgcg
     1141 gtcggggtcg gaatgcggtc caacgcgggc atcgccgcga ccatgttcca gacgctggcc
     1201 gaccggggaa tcaacatcct cgccatcacc acctccgaga tcaaggtgtc ggtgctgatc
     1261 gccgaggaat ataccgagct cgcggtgagg gtgcttcaca ccgcctacgg gctggacgat
     1321 ctgggggatg aggaatgagt ccgatgaggg gtggaatgag ttttatcgtc atcccggcga
     1381 aagccgggac cccaggacaa gaaggcacgg tgggtctccc cgaggtcccg gctttcgccg
     1441 ggatgacggc atgagcgaca ccctcggcca cgagcgcctc cagcggctga tgaagcgcgg
     1501 caccgacttc ctcggctgcg agatcgcgat cctcggcggg gcgatgagct ggatctccga
     1561 gcgcaacctc gtctcggcgc tctccaacgc cggcgccttc ggtgtgatcg cctgcggagc
     1621 gatgaccccg gatctgctcg ataccgagat cgcggagacg aagaagcgga cgaacaagcc
     1681 gttcggggtc aacctcatca ccatgcatcc gcagttgagc gagctgatcg acgtctgcgc
     1741 gaaccacgag gtaagccacg tcgtcctcgc cggcggtctt cctccgcccg ggtcgatcga
     1801 ccggatcaag ggcaacggcg ccaagctcat ttgcttcgcg ccggccctgg cgctggccaa
     1861 gaagctcgcc cgctccgggg tggacgcttt ggtcatcgag gggatggagg cgggcgggca
     1921 tatcggcccg gtctcgacct cggtgctggc ccaggagatc ttgccgccgc tcgccgcgga
     1981 catgccgatc ttcgtcgccg gcggaatcgg ccatggcggc ctgatggcgg gctatctgga
     2041 gatgggagcg gtgggctgcc agctcggcac ccgcttcgtc tgcgcgaccg aatgcatcgc
     2101 ccaccccaat ttcaagaagg ccttcatccg ggcctccgcg cgcgacgcca tcacaagcgt
     2161 ccagatcgat ccgcggctgc cggtcatccc cgtccgggcg ctgaagaacc gcgagatgga
     2221 aagcttcgcg gccaagcagc gcgaggtggc gcagcggctc gattcgggcg aggtcgagat
     2281 gatggaggcc cagctccaga tcgagcatta ttgggccggg gcgctgaggc gggcggtgat
     2341 cgagggcgac gtcgagacgg gctcggtgat ggccggccag tcggtcggga tggtcaagga
     2401 agaggcgccg gtggcggaga tcctcaagca gctggtcgac gaggccgccc aggcgctgga
     2461 gcggcgttcg gcctagtccc gccggtacgg gttgcggata gtcaacgtgc cttcgacaat
     2521 taagccgtgg tgcatgtcct ccgaccagag ggtgctgcac cctgccagca atgcggaggc
     2581 gacaacggcg gaatcataga catccagctt gtagcgctca gccaagaaga gcgtctgctc
     2641 gtgagtttcg agggtcagtg gctcgacgat gaaaaaggaa cgtacgagtt ccagcagctc
     2701 gtgcacatca ggccaggcga ggcgaatctt gcggcgggcg accgccgcca cttcgttcag
     2761 cacctgagcg ctaatccatc cgccctcacc gataagatct cgtgagcgtg ccgctttctc
     2821 ccggtcccga tctgctaaat agatgacgac attggtatcg agactagcga gcatatgcgt
     2881 cgctgcggtc gaacttatac cctgcgggca gcaacccccg gaaccgcttc aggcgttcga
     2941 gcgcctcctc tcggctcatt cgtcgggcaa tgccgatcac gggcttttca gctggcgcga
     3001 gctcaatctc atccccctct ttgagttcga gggcctcggc cacgtccttg gggatacgga
     3061 ttgcgaggct gttgccccat ttagccacct tcatattcga ttccatgata tacggttgag
     3121 cctatgtaga tcatagcgtg attgcggcgg tcgcacaatc tcgcgagggt cccgtacgac
     3181 acgttgacct caagcatggg cgccgcgccc agcgctccct cactccaggg agtcgatgca
     3241 ggcgggttcg tccttgcggc aattgtagaa ggtgatcggg attcggaccc aggccttgta
     3301 gtcgggaatt ccgtcgcgca tggcggcgcc gatcggttgc ctccccatcg caagatgggg
     3361 agggggacca ccgaaggtgg tggaggggtg acaaggcgcg ggccttcccg tctcgcgcag
     3421 gaaccgcata gtgcttcgaa ggtcacccct ccaccatgct tcgcatggtc cccctcccca
     3481 tgctgcgcat ggggaggcaa agtggcgtgg aaacccgccc cccttcctgc taccgctccg
     3541 caatgcccgc ccagtccgcc gcttcagccc gcgagatatt gaccgggctg cacgacgtga
     3601 tggcgtctcg ggccagcgcc caggccaagc tcaacaaggt ggtgaagatc atcgccgagg
     3661 cgatggcgag cgaggtctgc tcgatctatc tgcttcgcga cggggtgctc gagctgttcg
     3721 cgacccttgg gctgaaccag agcgcggtcc acgtcaccaa gctggcgctc ggcgagggcc
     3781 ttgtcggaac gatcgccaag gacgtctcga tcctcaatct cgcggaagcg ccaagccatc
     3841 ccgaattcgc ctaccggccg gagacgggcg aggagctgtt ccacagcttc gcgggggtgc
     3901 cgatcgttcg ccgcgaaagg gcgatagggg tgctcgccgt ccagcatgcc gaggcaaggg
     3961 cttatgacga cctcgagatc gaggcgctcc agacggtggc gatggtgctg gccgagctga
     4021 tcgccaacgc gggcctcgcc gacaaggcgg cggcggcggg gtcgacgacc cggggcgacg
     4081 ggccggagcg gctcgacggt ttgaagctgg tcgccgggat ggcgcggggg caggcggtct
     4141 atcaccagcc ccggatcctc atcgagcata ccgtcgccga ggacgtcgag gccgagcgcc
     4201 accgggtcta ttccgccttc cgcaagatgc gcgagcagat cgacaacatg accaacctcg
     4261 ccgagttcgg aacggcgggc gagcatcagg aggtgctcga gacctacaag atgttcgcct
     4321 atgacgaagg ctggtcgcgc cggatcaacg aggcgatcga cagcggcctc accgccgagg
     4381 cggcgatcga gcgggtgcgg cagcgcatgc agatgcggat gcgcgagatc gacgacagcc
     4441 tgctccagga ccggatgcac gacctcgacg atctgtcgaa ccggctgctc cggatcgtcg
     4501 ccgggcagct cggaaccgcg gctcaaatgg ggctgccgca cgacgcggtg ctgatcgccc
     4561 gcaacctcgg gcccgccgaa ttgctcgaat atgacaggcg caagctcaag ggcgtcattc
     4621 tcgaggaagg gtcgctgacg gcccatgtca ccatcgtcgc ccgggcgatg ggcgtgccga
     4681 tgctggggcg cgccggcaac gtgcgccaga cgatcggcga gggcgacgag gtactgctcg
     4741 acggcgacgc cggggtcgcc ttcgtcaggc ccacgggcgc ggtcgaggag gcgttcaagg
     4801 ccaagttcct gatcaccaag aagcgccgcg ccgaattcgc ggcgcttcgc gacctgccgc
     4861 cggttacgac ggacgggtcg cggatcaccg taatggtcaa tgccgggctt cgcgacgacg
     4921 tggcggcgct cgagatgatg ggcgccgacg ggatcggcct gttccggacc gagttccagt
     4981 tcctggtctc ggcgaccctt ccgagccgcg agagccagca gcggctgtac aaggccgtgc
     5041 tcgacgcggc cggcgacaag ccggtcatct tccgcaccgt cgacgtcggc ggcgacaagg
     5101 ccctgcccta tatcgacatg ggcgacggcg gcatcgagga gaatccggcg atgggctggc
     5161 gggcgctccg cctcgccctc gaccgcgatg gcctgatgaa ggcgcaggcc cgagctttgc
     5221 tcggcgccgc cgccggccgc gaattgaacg tcatgttccc gatggtctcg gagccctggg
     5281 aatatgacga ggcgcgggct ctggtcgagc agcagcgcga atggctggcc gggcacaaga
     5341 agaggcttcc gaccagcatc cgctatgggg cgatgcttga ggtgccggcg ctggccgaga
     5401 ccctcgacat cctcctgccc aaggtggatt tcctctccat cggcaccaac gacctcaccc
     5461 agttcctgtt cgccgccgac cgcgcccatc ccaagctggc ggagcgctac gactggctga
     5521 gcccgtccat cctccgcttc ctgaggcgaa tcgtccgaga ggccgatgcg gcgggcgtgc
     5581 cggtgggcgt gtgcggggag atgggcggac ggacgctgga ggcgatggct ctgatcggca
     5641 tcggcctcac ccgattctcg atcactccgg ccgcggtcgg cccgatcaag gcgatgatca
     5701 ggtcgctgga ccgggccgct ctgttgccgg tgatggagga gctgctggcg cggccgccgg
     5761 ccgatctgcg gggcgcgctg gcgcagtggg cggaagggcg gggggtcaag gtgtagcgtc
     5821 atcccgcgaa ggccgggatt catgaacacg gcggcatgag gtttcgaccg tccgtgttca
     5881 tggattccgg ccttcgccgg aaggacgagg aggtcccggc tttcgccggg atgacgggag
     5941 gagggctatc agccgtgccg cagtccggtt gactttgccc gcgctccccg gctctatgga
     6001 tcaatccaaa atcgagggga aatcggaaac tttatggcgg atgcggacca ggatcagttg
     6061 ctgggccagt cggtcggcga acggcttcga atcgctcgcg aggccaagaa gctgagcctg
     6121 gacgacgtcg cccgccagac ccgaatcccg gtccgtcacc tcgagcatat cgagcgggag
     6181 gagtgggacg cgctccccgc catcacctac agcgtcggct tcgctcgaag ctacgccaat
     6241 accgtcgacc tcgacggcgc caagatcggc gccgaggttc gccagcaatt gggcggaatc
     6301 cgcgccggcc acacggcggc cgcgcccttc tacgagccga cggacccggc gcgggtgccg
     6361 ccgcgctcca tcgccatcgc cgcggcggtg atcgccgtcc tgctcgtcat cggctatctg
     6421 gtctggcgca gcggcgcggt cggcgaggaa agcccggccg acgtcgccat cacccaggcg
     6481 gacgagccgg ccgcggccaa ccaggccgcc gcccgcccgg cccggccggt cgtcgccgcg
     6541 ccgccgccga gcaacgggcc ggtggtgctc accgccgcgg aggacgtctg gctgcgcatc
     6601 gaccaggccg gcggcggggc gccgctctac atggggaccc tgaagggcgg cc
//
LOCUS       DASWNU010000165         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_214149, whole genome shotgun sequence.
ACCESSION   DASWNU010000165 DASWNU010000000
VERSION     DASWNU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214149"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1745)
                     /locus_tag="VGB57_03260"
     CDS             complement(<1..>1745)
                     /locus_tag="VGB57_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEY0130398.1"
                     /translation="PVEKKERRELAISDVGEAGPGDLVLAEKVGRPPRITARVSQVLG
                     DPFAPRSFSLIAIHKHGIPHEFPEQVIVEAERVAEQPLGKREDLTHIPIVAIDPADAR
                     DHDDAVWAAPDDDPGNEGGWRAIVAIADVSFYVRSGSALDKEARKRGNSVYFPDRVVP
                     MLPETLSAEICSLKEGEDRAALVCHLQVSKEGQLKSWRFTRARVRIAANIAYEDAQAA
                     IDATIGQERIEVSSPACSMPEIEPDEPAVDPALVESTLKPLWACWNALFAAREKRAPL
                     DLDLPERRVVLDEKGRITSVAPRERLDAHKLIEDYMIAANVAAAKALESRKAPVMYRD
                     HEPPGREKLVALKDYLKTFGVEFALGQVIQPATFNRIIERIGEADFRPQVMEQILRTQ
                     TQAYYSPDNKGHFGLALGSYAHFTSPIRRYADLVVHRALVRAYGLGEGGLTDQEAEAM
                     EVTGELISQLERRAMEAERETLDRYVAAYLSERVGDLVDCRITGVQPFGFFATVEGLG
                     GDGLVPVSTLGSDYYRYEESSQSLVGDDSGERFSPGQRLKLRLVEANPVSGGLRFGLP
                     DGGGTPARPSGGPRR"
BASE COUNT          247 a          598 c          617 g          283 t
ORIGIN      
        1 cgcggcgcgg gccgccgctg ggccgggccg gagtgccgcc gccgtcggga agcccgaacc
       61 tcagcccccc ggagaccgga ttggcctcga ccagccggag cttcagccgc tgaccggggc
      121 tgaagcgctc gccgctgtcg tcgccgacga ggctctggct cgactcctca tagcggtaat
      181 aatccgagcc cagcgtggag accgggacga ggccgtcgcc gccgaggccc tcgaccgttg
      241 cgaagaagcc gaacggctgg accccggtga tccggcagtc gacgaggtcg ccgacccgct
      301 cggagagata ggcggcgacg tagcggtcga gggtctcgcg ctccgcctcc atcgcgcgcc
      361 gttcgagctg ggagatgagc tcgcccgtca cctccatcgc ctcggcctcc tggtcggtga
      421 ggccgccttc gccgaggccg taggcgcgga ccagggcgcg gtggacgacg aggtccgcat
      481 agcggcggat ggggctggtg aaatgggcgt aggagccgag cgccaggccg aaatggccct
      541 tgttgtccgg ggaataataa gcctgcgtct gggttcgaag gatctgctcc atgacctgcg
      601 ggcggaagtc ggcctcgccg atccgctcga tgatccggtt gaaggtggcc ggctggatga
      661 cctggccgag cgcgaactcg accccgaacg tcttcagata atccttgagc gcgaccagct
      721 tctcgcgccc gggcggctcg tggtcgcggt acatgaccgg cgccttcctc gactccagcg
      781 ccttcgccgc ggcgacgttg gcggcgatca tataatcctc gatcagcttg tgcgcgtcga
      841 gccgctcgcg cggcgctacc gaggtgatcc gtcccttctc gtcgagcacg acccggcgct
      901 cgggaaggtc cagatcgagg ggggcccgct tctcgcgcgc ggcgaagagg gcattccagc
      961 aggcccagag cggcttcagg gtcgattcga ccagggcggg atcgaccgcc ggttcgtccg
     1021 gctcgatctc gggcatcgag catgccggcg aactgacttc gatccgctcc tggccgatcg
     1081 tggcgtcgat cgccgcctga gcgtcctcat aggcgatgtt ggcggcgatg cggacccggg
     1141 cgcgggtgaa gcgccagctc ttgagctgcc cctccttcga cacctgcagg tggcagacga
     1201 gggcggcccg gtcctcccct tccttcagcg agcagatctc ggcgctgagc gtctcgggga
     1261 gcatcggcac cacccggtcg gggaagtaga cgctgttgcc gcgcttccgc gcttccttgt
     1321 ccagcgccga gccggagcgg acgtagaagc tgacgtcggc gatggcgacg atcgcccgcc
     1381 acccgccttc gttgcccgga tcgtcgtcgg gcgcggccca gacggcatcg tcatggtcgc
     1441 gggcgtcggc ggggtcgatg gcgacgatgg ggatgtgtgt cagatcctcg cgcttgccga
     1501 gcggctgctc cgccacccgc tccgcttcca cgataacctg ctcggggaat tcgtgcggga
     1561 ttccgtgctt gtggatggcg atcaggctga agctgcgcgg tgcgaacggg tcgcccagca
     1621 cctgcgagac acgcgccgtg atccgcggcg gccggccgac cttttccgcg aggacgagat
     1681 cgccggggcc cgcctcgccg acatccgaga tcgccagctc gcgccgctcc ttcttctcga
     1741 ccggc
//
LOCUS       DASWNU010000166         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_110589, whole genome shotgun sequence.
ACCESSION   DASWNU010000166 DASWNU010000000
VERSION     DASWNU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110589"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..427
                     /gene="gatC"
                     /locus_tag="VGB57_03265"
     CDS             140..427
                     /gene="gatC"
                     /locus_tag="VGB57_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0130399.1"
                     /translation="MSVDAATVRHIAKLARIAVSDAEVEALAPELNNILGWVEQLQEV
                     DVEGVEPMTAVIPNKLRLRDDEVTDGGKRDDVLANAPAAEHGFFAVPKVIE"
     gene            531..2012
                     /gene="gatA"
                     /locus_tag="VGB57_03270"
     CDS             531..2012
                     /gene="gatA"
                     /locus_tag="VGB57_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY0130400.1"
                     /translation="MTNLTDLGIAAIRDGVRDGAFSAREIASAFSAAVEKGRGLNAFT
                     VETPDHALAAADAADSARASGDLKPLSGVPLGIKDLFATEGVATTAGSRILEGFEPRY
                     ESTVSANLFAAGAGMLGKLNMDEFAMGSSNETSAWGPVLSPWRRSDGGNAALTPGGSS
                     GGSAAAVAARLAPGVTGTDTGGSIRQPAAFVGITGIKPTYGRCSRWGVVAFASSLDQA
                     GPMARDVRDCAILLQAMAGFDPKDSTSLDLPVPGWEAALSSDVRGKRIGIPKEYRIEG
                     VPAEIDRVWERGIELMRDAGAEIVEVSLPHTKYALPTYYIIAPAEASSNLARYDGVRY
                     GLREAPENGNLTDMYEATRAAGFGAEVKRRIMIGTYVLSAGFYDAYFNRAQKVRALIA
                     RDFERVWGECDLILTPTAPSAAFALGEKSADPIAMYLNDVFAVPASLAGLPAISVPAG
                     LDDQGLPLGLQLVGRALDEQGVLNAALALEERTGFTARPEAWW"
     gene            2118..>2852
                     /gene="gatB"
                     /locus_tag="VGB57_03275"
     CDS             2118..>2852
                     /gene="gatB"
                     /locus_tag="VGB57_03275"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006960058.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEY0130401.1"
                     /translation="MSDYRIHGATGEWEVVIGLEVHAQVTSNAKLFSGASTEFGAEPN
                     SQVSLVDAAMPGMLPVPNAECIRQAVRTGMALEARINPWSRFDRKNYFYADLPQGYQI
                     SQLYHPIVGEGEIEILLDEKDESSAKRVGIERIHLEQDAGKLMHDQHPSFSYVDLNRS
                     GVALMEIVSRPDMRSPEEAGAYVRKLRSILRYVGSCDGNMEQGSMRADVNVSVRKPGA
                     EFGTRTETKNVNSVRFIMATIEHEASR"
BASE COUNT          485 a         1015 c          930 g          422 t
ORIGIN      
        1 cagcatcaaa tcctccccgc ctgcatcgcc gctgcacaag cctaaggcac aggccgaagc
       61 aagcctttcc actatccggc gccggcacgg ccgacagcgc ttcttgccgc gctgcagcgc
      121 gaatgctagc gccgccgcca tgtccgtcga cgccgcaacc gtccgccata tcgcgaagct
      181 cgcccggatc gccgtctccg acgccgaggt cgaagcgctc gcgccggagc tgaacaacat
      241 cctcggatgg gtcgagcagc tccaggaggt cgacgtcgaa ggagtcgagc cgatgaccgc
      301 cgtcattccg aacaagcttc ggctgcggga cgatgaggtt acggacggcg gcaagcgcga
      361 cgacgtcctc gccaacgcgc cggcggcgga gcacggcttc ttcgccgttc ccaaggtgat
      421 cgaatgatgg agcaccttct cgtcgcccca gcgaaggctg gggtcccggc gggcgaaggc
      481 cgccggcgcc tttccccccc cgggatccca gccttcgctg ggatgacgct atgacgaacc
      541 tcaccgacct cggaatcgcg gcgatccgcg acggggtgcg cgacggcgcc ttctccgccc
      601 gcgagatcgc ctcggcgttc agcgccgccg tcgaaaaggg cagggggctc aacgccttca
      661 ccgtcgagac cccggaccac gcgctcgccg cggccgacgc cgccgactcc gcccgcgcct
      721 cgggcgatct gaagccgctc tccggggtgc cgctcgggat caaggacctg ttcgcgacgg
      781 agggcgtcgc caccacggcc gggagccgga tcctcgaagg cttcgagccc cgctacgaat
      841 ccacggtcag cgccaacctg ttcgcggcgg gcgccgggat gctcggcaaa ttgaacatgg
      901 acgagttcgc catgggctcg tccaacgaga ccagcgcctg gggaccggtc ctctcgccct
      961 ggcggcgaag cgacggcggc aatgccgcgc tcaccccggg aggaagctcc ggcggctcgg
     1021 cggcggcggt cgcggcgcgc ctggcgccgg gagtcaccgg caccgatacc ggcggctcga
     1081 tccgccagcc cgccgccttc gtcgggatca ccgggatcaa gccaacctac ggccgctgct
     1141 cgcgctgggg cgtggtcgcc ttcgcctcga gcctcgacca ggccgggccg atggcgcgcg
     1201 acgtccgcga ctgcgcgatc ctgctccagg ccatggccgg cttcgacccg aaggattcga
     1261 cctcgctgga cctgccggtg cccggttggg aggctgcgct gagcagcgac gtgcgcggca
     1321 agcgcatcgg gatcccgaaa gaatatcgga tcgagggagt cccggccgag atcgaccgcg
     1381 tctgggagcg cggcatcgag ttgatgcgcg acgccggggc cgagatcgtc gaggtctcgc
     1441 tgccccacac gaaatacgct ctgccgacct attacatcat cgccccggcc gaggcctcgt
     1501 ccaacctcgc ccgctacgac ggggtccgct acggcctccg cgaggcgccg gagaacggca
     1561 acctcaccga catgtacgag gcgacccgcg ccgccggctt cggcgccgag gtcaagcgcc
     1621 ggatcatgat cggcacctac gtcctctcgg ccggcttcta cgacgcctat ttcaaccgcg
     1681 cccagaaggt gagggcgctg atcgcccgcg atttcgagcg cgtctggggc gaatgcgacc
     1741 tcatcctcac gcccacggcg ccctcggcgg ccttcgccct gggcgagaag agcgccgatc
     1801 cgatcgccat gtatctgaac gacgtcttcg ccgtcccggc aagcctcgcc ggcctccctg
     1861 cgatctccgt ccccgccggc ctggacgacc agggtctccc gctcggcctc cagctggtgg
     1921 gcagggcgct ggacgagcag ggagtcctca atgccgctct ggcactggag gaacgcaccg
     1981 gattcaccgc taggccggag gcatggtggt gatccggacc cgtcattgcg aggagcgaag
     2041 cgacgcggca atccagcggg cctcggagac cgtcctggat tgcttcgctg cgctcgcaat
     2101 gacggtggag gtggcacatg tctgattatc gaatccacgg cgccaccggc gaatgggagg
     2161 tggtgatcgg cctcgaggtc cacgcccagg tcacgtcgaa cgccaagctc ttctccggcg
     2221 cttcgaccga gttcggggcc gagcccaaca gccaggtcag cctggtcgac gccgccatgc
     2281 cgggaatgct cccggtcccc aacgcggagt gcatccgcca ggcggtgcgc accggaatgg
     2341 cgctggaggc gcggatcaac ccctggtcgc gcttcgaccg gaagaattat ttctacgccg
     2401 atctgccgca gggctatcag atcagccagc tctaccaccc gatcgtcggc gagggcgaga
     2461 tcgagatctt gctcgacgag aaggatgaga gcagcgccaa gcgcgtcggc atcgagcgca
     2521 tccatctcga gcaggatgcc ggcaagctga tgcacgacca gcatccgagc ttctcctatg
     2581 tcgatcttaa ccgctcgggc gtcgccttga tggagatcgt ctcccgtccg gacatgagga
     2641 gtcccgaaga agcaggcgct tatgtcagga agctgagatc gatactgcgc tatgtcggca
     2701 gctgcgacgg caacatggag cagggctcca tgcgcgccga cgtcaacgtc tccgtccgca
     2761 agcccggcgc cgaattcggc acccgcaccg agaccaagaa cgtcaattcg gtccgcttca
     2821 tcatggccac gatcgaacat gaggcaagcc gc
//
LOCUS       DASWNU010000167         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_57788, whole genome shotgun sequence.
ACCESSION   DASWNU010000167 DASWNU010000000
VERSION     DASWNU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57788"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1265)
                     /locus_tag="VGB57_03280"
     CDS             complement(135..1265)
                     /locus_tag="VGB57_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0130402.1"
                     /translation="MRPRYPESREEKAARLLRSAGQMKAASSAAVESAGGGSGGDEIA
                     SKISARLASAGIAEAAAAPLARMSVDTARATLDAAARGELSPADVSTRQLASLEAVIS
                     VTGRPAWFVRNNVPVAEGGVGDRASEYWLVHLSNLHDKIKEACSRVGAIFMEEDGRRI
                     AIGTGWIIEDGIVATNAHVARHLYFRKPVVAANDPADGWRKRGGVTGLIDLAFENGIE
                     RSRLFRFSRPCFIESDEAARPDLAIIALEPVAGQAFPRRIDVETRADRPGAEALVYVV
                     GHPAADLNDDANVTAVFGALDGTKRISPGKFLGLLGSFVIGHDCSTVNGSSGSPLLDF
                     GTGLVIGHHYWGEPGARNEAVFMPAIIGHPAVAKTLAGGFRS"
     gene            complement(1262..1531)
                     /locus_tag="VGB57_03285"
     CDS             complement(1262..1531)
                     /locus_tag="VGB57_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0130403.1"
                     /translation="MKRVAQRLRLRDAGGKPCAGITVAVRDAAGPVPELGYVTGDDGE
                     VQIGLPPGSATLEALLSDGRRHCFAIEASGEPGRCHKVQLDGEQE"
     gene            complement(1547..2455)
                     /locus_tag="VGB57_03290"
     CDS             complement(1547..2455)
                     /locus_tag="VGB57_03290"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY0130404.1"
                     /translation="MSTPYKSISSAAMSDEEMIPGRENAVLQETDGSLGADEAVSSEA
                     DLIVSGAREEPLPRPTPRVGTETVLGPDDRVRITETDRLPWRMIAALRLIPRPPLTAA
                     YIGTGWFIGRRTLLTAGHCVFSESDFGGWIGRIEVSPGRNGSQFPFGTANAVRFSALA
                     EWQASSDPDFDVGCIHLDEPVGDRVGYFKLAALSDADLQNRLLNVSGYPGDRGQGKQQ
                     FFHVNRVLHPAARRIYYDIDTYQGQSGSPVFIQDDPAEPPAGVGVHAYGVGGTPSSLG
                     ITANSAPRITPQLLATIREWLADDDR"
     gene            2696..3289
                     /locus_tag="VGB57_03295"
     CDS             2696..3289
                     /locus_tag="VGB57_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130405.1"
                     /translation="MGDEQTYEPGGQTYEAGERPDEELAREISPENDADLHADPDAPS
                     GEAGPATEAARPWRVAKALLRLREQVNALAPNRSKVSDGTIGDTNHQNSNSDHNPHVA
                     DAGVGVVTAMDITHNPPGGCDSAKIAQSLVASRDPRIKYIIWNRNIVASYPAGGRPAW
                     TWRPYGGRNPHDHHVHISVLGAKAQFDSQSDWAVQVR"
     gene            3345..4445
                     /locus_tag="VGB57_03300"
     CDS             3345..4445
                     /locus_tag="VGB57_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130406.1"
                     /translation="MGEPVAGGDDDANVDEDRQRAAAAGGAAPPPRQQAASSGLASAG
                     VSGPSPGAMSSGSAGGIEIELLTYQAVERLVGAVAQACEGTAGKILLVTPSDLMQAAG
                     ARLAYLLLSDLTERMSRLYELKSGRESATEAGAAPDPVAALGAVVKLGQDIVTTVGIT
                     EARTGHSVAVDERGVAVLAARALSKCGLKSVVLAEPGAWSDPAAAKGVLAKAQAAALA
                     LRQLGDKGGEEAGALLDRYRELLDQAAAAGPALVAGFATLAAMSEEDAKVLTIRVLTA
                     GGTIWEKRHIGTLLGLSDPFSFSAGAVISYSFVNARTGEIISADTLYDWTGRNSVPRE
                     TRLASLTNVPRVSKTDPPRSDRATFPSGAAGK"
     gene            complement(4481..>5377)
                     /locus_tag="VGB57_03305"
     CDS             complement(4481..>5377)
                     /locus_tag="VGB57_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130407.1"
                     /translation="SLSESSIEVGSGGDRVEALLITLDGLPPAGIDPPSVDIPGYIAQ
                     RTSTATGFTFTYVASEGDPDAAGSKMAYLPAEKGQQIGQRKITLPGLNILAAQNASSS
                     GWILRNQHLVERRTTVESFLYQTSPVSFPTPLRPTFVVLEEIDIAAIPTGGPVARTLA
                     EQLGGLFTALFAEAPAGPQEIQLTICYSVSVNPALPPIALPVVFVPPIAFLPDNEFNS
                     DAEDGDPLSQAALSQKIATAITTWYQTNAPLQGGTLTFDLKVMTRQPPGGGSAPPTPL
                     LELAYLVLNYADWTDHPADQVN"
BASE COUNT          864 a         1760 c         1881 g          872 t
ORIGIN      
        1 atttgcgagg gtcgcctcga tccgcgtcgt cgggcggcat gacatcggcg acggccgcat
       61 agacgttcgc catcgcgacg tagttgcgga tcactttcgg gaagacatcg gggtggaggg
      121 ggctgaccat ccgctcagct cctgaacccg ccggcgagcg tcttcgcgac ggcgggatgg
      181 ccgatgatcg ccggcatgaa aaccgcctcg ttgcgcgcgc cgggctcgcc ccaataatga
      241 tggccgatca cgaggccggt gccgaaatcc agaagcgggc tgccggagga gccgttcacc
      301 gtcgagcagt cgtggccgat gacgaagctg ccgagcaggc cgaggaactt gccggggctg
      361 atccgcttcg tcccgtccag ggcgccgaac acggcggtga cgttggcgtc gtcgttgaga
      421 tcggcagcag gatgtccgac gacgtaaacc agggcttcgg cgcctgggcg gtcggcccgg
      481 gtttcgacgt cgatgcggcg cgggaaggcc tggccggcga ccggttccag cgcgatgatg
      541 gcgagatcgg ggcgcgcggc ctcgtcgctt tcgatgaagc acgggcggga gaagcgaaaa
      601 agcctgctgc gctcgattcc gttctcgaac gcgagatcga tcagcccagt gacgccgccc
      661 cgcttccgcc agccgtcggc ggggtcgttg gccgcgacca ccggcttccg aaaatagaga
      721 tggcgggcga cgtgggcatt ggtcgcgacg atcccgtcct cgatgatcca cccggtgccg
      781 atcgcgatcc tcctgccgtc ctcctccatg aagatcgcgc cgacccggga gcatgcctcc
      841 ttgatcttgt cgtgcaggtt cgaaaggtgg acgagccagt actcggatgc acggtcgcca
      901 acgccgccct ccgccacggg cacgttgttg cggacgaacc aggccggtcg tccggtgacg
      961 ctgatcaccg cctcgaggct cgcaagctgc cgggtgctca catcggccgg gctgagctcg
     1021 ccccgggcgg cggcgtcgag ggtcgcgcgg gccgtgtcga ccgacatgcg cgccagcggc
     1081 gccgccgccg cctcggcgat tccggcggac gccagccggg cggagatttt cgatgcgatc
     1141 tcgtcaccgc cggatccgcc gccggccgat tcgaccgccg cgctcgaagc cgccttcatc
     1201 tgccccgcgc ttcgcaacag ccgcgccgcc ttttcctccc gggattcggg gtagcggggt
     1261 ctcattcctg ctcaccgtcc aattgaacct tgtggcatct gcccggctcg ccgctggcct
     1321 cgatcgcgaa gcaatggcgc ctgccgtccg acagaagggc ctcgagcgtc gcgctgcccg
     1381 gcggcaggcc gatctgcacc tccccatcgt cgccggtgac atagcccagc tccgggaccg
     1441 gacccgccgc atccctgacg gcgacggtga tccccgcgca gggtttcccc cccgcgtccc
     1501 tgagacgaag ccgttgggcg acgcgtttca ctgcctgcgg gcgcggttat ctgtcatcgt
     1561 cggcgagcca ctccctgatg gttgcgagaa gctgcggcgt gatgcgcggg gcggagttgg
     1621 cggtgatgcc gaggctcgac ggcgtgccgc cgactccata agcgtgcacg cccacgccgg
     1681 caggcggctc cgcagggtcg tcttgaatga agacgggaga gccgctctga ccctggtagg
     1741 tgtcgatgtc gtaatagatc cgacgtgcgg cagggtgcag cacgcggttg acgtggaaga
     1801 attgctgctt cccctggccg cgatcgccgg gatagcccga cacgttgagc agccggttct
     1861 gaaggtccgc atcggacagc gcggcgagct tgaaataacc cacccggtcg ccgaccggtt
     1921 catcgagatg gatgcagccg acgtcgaagt ccgggtcgct gctggcctgc cactccgcga
     1981 gtgccgagaa gcgcacggca ttggcggtgc cgaatgggaa ctggctccca ttgcggccag
     2041 gcgagacctc gatcctgccg atccagccgc cgaaatccga ttcgctgaag acgcagtggc
     2101 cggcggtgag caaggttctc ctgccgatga accagccggt tccgatatag gcggcggtga
     2161 gcggcgggcg cgggatcagc ctcaaggcgg caatcatccg ccatggaaga cggtcggttt
     2221 cggttatccg aacccgatcg tccggtccga ggacggtctc ggtgccgact cgaggcgtcg
     2281 gccgggggag cggctcctct cgagcgccgc tgacgatcag atctgcctcc gaggaaaccg
     2341 cctcgtccgc gccgaggctt ccgtcggttt cctgaagcac cgcgttttcg cggcccggga
     2401 tcatctcctc gtcgctcatc gctgcactgg aaatgctttt gtacggtgtc gacatttccc
     2461 ttcccctctt ttctacagcc aactccctcg atgttgctcg acgactcgtc gcttgcttgc
     2521 cggtgctcgg aacgactcgg ccaatctaat tcacacgcgt tgagctagct acaagattcg
     2581 gatatagtgg agcgttgctg accgagcacc tgtgcagcgg ctgtttgaca ataactacct
     2641 gtagccccgt caattgaggg cgcgcgaggg tgcgcgccgc atcttggaga gagccatggg
     2701 cgacgaacag acctacgaac ccggcgggca aacctacgaa gccggcgaac gtcccgatga
     2761 agagctggcg cgcgaaattt cgcccgagaa cgacgcggac ctgcatgccg atccggacgc
     2821 gccgtccgga gaggccggcc cggccaccga agccgcgcgg ccgtggcggg tggcgaaggc
     2881 cttgttgagg cttcgcgagc aggtcaacgc cctcgccccc aaccgaagca aggtgagcga
     2941 cggcacgatc ggcgacacga accaccagaa cagcaattcg gaccacaacc cgcatgtcgc
     3001 cgacgcgggc gtgggcgtgg tgacggcgat ggacatcacg cacaacccgc cgggcggctg
     3061 cgactcggcc aagatcgcgc agtctctcgt ggccagccgc gatccgcgga tcaaatacat
     3121 catctggaac cggaatatcg tggcctctta ccccgcgggc ggacggccgg cctggacctg
     3181 gcgcccttat ggggggcgca atccgcatga ccatcacgtc catatctcgg tactcggcgc
     3241 gaaggcccag tttgactcgc aaagcgactg ggcggtgcag gtgcgatgaa gggttcgggc
     3301 gggcggtcgg cgcggatccc tgagtcgtca tcgaggaggc ggtgatgggc gaaccggtcg
     3361 caggcggcga cgacgacgcc aatgtcgacg aagaccgcca gcgcgccgcc gccgccggcg
     3421 gcgcggctcc ccctcctcgg cagcaggcgg cttcgtcagg gctcgcgagc gcgggcgtga
     3481 gcgggccttc gccaggagcc atgtcgtccg gatcggccgg cggcatcgag atcgagctgc
     3541 ttacctacca ggccgtcgag cgactcgtcg gcgcggtggc ccaggcgtgc gaggggacgg
     3601 cggggaagat actcctcgtc accccctccg acctgatgca ggcggcgggc gcgcgtctgg
     3661 cttacctgct gctctccgac ctgacggagc ggatgagtcg cctctacgaa ctcaaatccg
     3721 gcagggagag cgcgaccgag gcgggggcgg cgcccgaccc ggtcgcggcg ctcggggcgg
     3781 tcgtcaagct gggccaggat atcgtcacca cggtgggcat caccgaggcg cggaccggcc
     3841 actccgtcgc cgtggacgag cgcggcgtgg cggtgctcgc ggctcgcgcc ttgagcaaat
     3901 gcgggctgaa gtccgtggtg ctcgccgagc ccggggcctg gtcggatccg gctgctgcga
     3961 aaggcgtgct cgcgaaggcg caggcggccg ccctggcgct gcggcagctc ggggacaagg
     4021 gcggcgagga agccggtgcg ctcctcgacc gctaccggga gcttctcgac caggcggccg
     4081 ccgccgggcc agcgctcgtc gcgggcttcg cgacccttgc cgcgatgagc gaagaggacg
     4141 ccaaggtgct tacgatccgg gtgctcaccg ccggcgggac gatttgggag aagaggcata
     4201 tcggcaccct cctcggcctc tccgacccct tcagcttcag cgccggtgcg gtgatcagct
     4261 attcgttcgt caacgcccgg acgggcgaga tcatctcggc ggacactttg tacgactgga
     4321 cggggcgcaa cagcgtaccg cgcgagacgc gcctcgcctc cctcaccaat gtgccgcgcg
     4381 tgtcgaagac ggacccgccg aggtccgacc gagccacttt cccgtcaggc gcggcaggca
     4441 agtaattcgc ttgtgatcgg tccgtgcggc cgcggcgggg ctaatttacc tggtccgccg
     4501 ggtgatccgt ccaatccgcg tagttgagta cgaggtaggc gagctcgagc agcggggtcg
     4561 gcggcgcgct gccgccgccg ggcggctggc gggtcatgac cttgaggtcg aaggtcaatg
     4621 tgccgccctg gagcggagcg ttggtctggt accaggtcgt gatcgcggta gcgatcttct
     4681 ggctgagcgc ggcctggctg agcggatcgc cgtcctccgc gtcggaattg aactcgttat
     4741 ccggcaggaa ggcgatcggc ggtacgaaga cgaccggcag ggcgatcggc gggagcgcgg
     4801 gatttacgct gaccgagtag catattgtca gctgtatttc ctgcgggccg gcgggcgcct
     4861 cggcgaacag agccgtgaac agcccaccga gttgctcggc cagggtccgg gccaccggac
     4921 cgcccgttgg gatggcggcg atgtcgatct cctcgaggac gacgaatgtc ggccgaagcg
     4981 gcgtcgggaa gctgaccggg ctggtctgat agaggaacga ctccaccgtc gttctacgct
     5041 cgacgaggtg ctggttgcgc aaaatccacc cgctcgatga agcattttgc gcggcgagga
     5101 tgttgaggcc gggcagtgtg atcttccgct ggccgatctg ctgccccttc tccgcgggaa
     5161 gataggccat cttcgaaccg gcagcgtccg gatcgccctc cgaggcgacg taggtgaaag
     5221 tgaaaccggt cgcggtcgaa gtgcgctgcg cgatgtagcc ggggatgtcg acgctcgggg
     5281 gatcgatccc ggccgggggg agcccgtcga gagtgatgag cagcgcctcg acccgatcgc
     5341 cgccggatcc gacctcgatc gaactctcgg acaggct
//
LOCUS       DASWNU010000168         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_421828, whole genome shotgun sequence.
ACCESSION   DASWNU010000168 DASWNU010000000
VERSION     DASWNU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421828"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2134
                     /locus_tag="VGB57_03310"
     CDS             <1..>2134
                     /locus_tag="VGB57_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130408.1"
                     /translation="GGQSLTGNDADNVIDGGAGNDSLEGGAGIDTARYAGATTVTQSG
                     TGWTVTDAGGTDTLSNIERIDDGGAGRTLLVGNGGYATIQEAIDAASNGDTILVASGT
                     YTENLDVNKDVTILGPNRGVAGTGTRAAEAVIDGQIVINAAGATLDGFRLVGDAAGPL
                     GTTAVEVKANNFTLSNSVLDGSGDFAIFVGLVTGLDIGRNLIRGYSIGAYVAGGNTSG
                     SIHDNRFQGNGGPETGLGNGINSETSHVSIANNVFDGIYAGSLNLYPFGPDSVDLNSY
                     VIGNTITNSGPARPVQILPTNLTHNIVGTDFNEAFDGETAAARDGVTGAFSFDGRGGD
                     DRAWGGEEGDTLSGGTGNDQLFGNGGADSLSGGDNNDTISGGAGADTAQGGNGNDTLN
                     GGDGDDSLSGGAGVDTLNGDLGNDTLHGGTGNDSLHGGGGTDKAVYDGHRGDYSITMA
                     TGAGGRIVGFAAVSDNDFSNGNDGVDSLISVEVLQFSNRTFDSTMPVQLFDQNNQLIA
                     TFTTIQSAINVAQDGYTIRLAAVTFDEDLVINVGVTIRGIRTTTTGNAAGRDPANGVG
                     EATIIGHHKVTSEANVTLLGLRFLNDSTTTGGGPSNPTLQFLTGGSGLTGHSVTNSIF
                     WSAVAGGAANDRAISAPVMTTGQLTLTGNLISGSSQDLFGNASWGQGIWIDGGGIALN
                     ASGNVIEWTKTGLSLDGAGGSFYLVENNI"
BASE COUNT          409 a          744 c          672 g          309 t
ORIGIN      
        1 cggcggccag agcctcaccg gcaacgatgc cgacaatgtg atcgacggcg gcgccggcaa
       61 cgattccctc gaaggcggag ccgggatcga cacggcgcgc tatgccggag cgacgaccgt
      121 cacccagagc gggaccggct ggacggtcac cgacgccggc ggcaccgaca ctctcagcaa
      181 catcgagcgg atcgacgacg gcggcgcggg caggaccctg ctcgtcggca atggcggcta
      241 cgccaccatc caggaggcga tcgacgcggc ctcgaacggc gacaccatcc tcgtcgcctc
      301 ggggacctac acggaaaatc tggacgtcaa caaggacgtc accatcctcg gcccgaaccg
      361 tggcgtcgca ggcaccggca cccgcgcggc ggaggcggtg atcgacggcc agatcgtgat
      421 caacgcggcg ggcgcgaccc tcgacggctt caggttggtc ggggacgcgg cgggcccgct
      481 cgggactacg gccgtcgagg tcaaggcgaa caacttcacc ctgtcgaact cggtcctcga
      541 cggcagcggc gacttcgcaa tcttcgtcgg cctggtgacc ggcctcgaca tcggccgcaa
      601 cctgatccgg ggctattcga tcggcgccta tgtcgccggc ggcaacacca gcggctcgat
      661 ccacgacaac cgcttccagg gtaacggcgg gccggagacc ggcctcggca acggcatcaa
      721 ctccgagact tcgcacgtca gcatcgccaa caacgtcttc gacggcatct atgccggatc
      781 gctgaacctc tatccgttcg gccccgacag cgtcgatctc aacagctacg tcatcggcaa
      841 cacgatcacc aacagcggcc ccgcccgccc ggtgcaaatc cttccgacca accttaccca
      901 caatatcgta ggtaccgatt tcaacgaggc gttcgacggc gagacggcgg cggcccgcga
      961 cggagtcacc ggcgcgttca gcttcgacgg ccgcggcggc gacgaccgcg cctggggcgg
     1021 cgaagagggc gataccctca gcggcggcac cggcaacgac caattgttcg gcaatggcgg
     1081 cgccgacagc ctgagcggcg gcgacaataa cgataccatc tccggcggag ccggcgcgga
     1141 caccgcgcag ggcggcaacg gcaacgacac cctgaacggc ggcgacggcg acgattcgct
     1201 ttccggcggg gccggcgtcg atacgctcaa cggcgacctc ggcaacgaca cgcttcacgg
     1261 cggcaccggc aacgattcac tgcatggcgg cggcgggacc gacaaggcgg tctatgacgg
     1321 gcatcgcggc gactattcga tcaccatggc caccggcgcg ggtggccgca tcgtgggctt
     1381 tgcggcggtc tccgacaacg atttcagcaa cggcaatgac ggggtggatt ccctcatcag
     1441 cgtcgaggtg cttcaatttt ccaaccggac gttcgactcg accatgccgg tccagctgtt
     1501 cgaccagaac aaccagctca ttgccacctt caccacgata cagtcggcga tcaacgtggc
     1561 gcaggacggt tacacgatcc gcctcgccgc ggtcaccttt gacgaggatc tggtgatcaa
     1621 cgtcggggtg acgatccggg gcatcaggac caccactacg ggtaacgccg ctggccgcga
     1681 ccccgccaac ggggttggcg aggcaacgat catcggtcat cacaaggtca cctcggaggc
     1741 caatgtcacc cttctcggcc ttcgcttcct caacgactcg actacgacag gcggcggccc
     1801 ttccaatccc acgctgcaat tcctgaccgg cgggagcggg cttaccggcc actccgtcac
     1861 caactcgata ttctggtcgg cggtcgcggg cggtgcggcg aacgaccgag ccatctccgc
     1921 gccggtcatg acgacgggcc agctgaccct gaccgggaac ctcatctccg gctcgtctca
     1981 ggacctgttc ggcaacgctt cctggggcca ggggatctgg atcgacgggg gcggaatagc
     2041 cctgaacgcc tcgggcaacg tcatcgaatg gaccaaaacc ggcctctcgc tggatggcgc
     2101 cggcggctcc ttctacctcg tcgagaacaa catt
//
LOCUS       DASWNU010000169         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_291628, whole genome shotgun sequence.
ACCESSION   DASWNU010000169 DASWNU010000000
VERSION     DASWNU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291628"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGB57_03315"
     CDS             <1..375
                     /locus_tag="VGB57_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HEY0130409.1"
                     /translation="VSNKLNGIGGNDVLDGAKGNDILTGGAGGDQFRVGEIGYDDEIV
                     DFTSGLDKIFLAEIDANSGIAGNQAFTFIGNNAFSGVAGQLRTYSQDGDNFVAGDVNG
                     DGLADFTINVNNANVVVTDFFL"
     gene            648..1079
                     /locus_tag="VGB57_03320"
     CDS             648..1079
                     /locus_tag="VGB57_03320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY0130410.1"
                     /translation="MNETILRFGYPATLIAEYEHWVVLLRPAQPTLGSLVLAAKGEAT
                     SFADLRPQAFGELQRAVADVERALGAAIRFEKINYLMLMMVDPHVHFHVIPRYEGERS
                     ACGVGVKDAGWPKVPALGEAVTLEPEQVEALAGYFRGYWPE"
     gene            complement(1210..1941)
                     /locus_tag="VGB57_03325"
     CDS             complement(1210..1941)
                     /locus_tag="VGB57_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEY0130411.1"
                     /translation="MTIGKAIVLSAGKGSRLLPLTATRPKCLIDLNGRSLLEWQLDAL
                     QGAGVADIVVVTGFAEETVAEVTARRSGVRTLFNPFYHVADNLGSVWMARAELDRDTL
                     LLNGDTLISPRLLERVLEAAPGPIAVTVDEKESYDADDMKVLRSGDRLLRIGKALEAG
                     QYNAESIGLLAFRGEGPRLFADQVERMMRQPDGTRRWYLRAIDSLALAGADVSIVSIK
                     GEEWQEVDFPEDVEAAKALTARWAF"
BASE COUNT          357 a          643 c          709 g          356 t
ORIGIN      
        1 gtctcgaaca agctgaacgg catcggcggc aacgacgtgc tcgacggcgc caagggcaac
       61 gacatcctca ccggcggcgc cggcggggac cagttccggg tcggcgagat cggctatgac
      121 gacgagatcg tcgacttcac cagcgggctc gacaagatct tcctggcaga gatcgacgcc
      181 aacagcggca ttgccggcaa ccaggccttc acgttcatcg gcaacaacgc cttcagcgga
      241 gtcgcgggtc agctgaggac ctacagccag gacggcgaca atttcgtcgc cggcgacgtg
      301 aacggcgacg ggctcgccga cttcacgatc aacgtcaaca acgccaatgt cgtggtgacg
      361 gacttcttcc tctaggagcg aggacggtcg cggccggatg caaaaagcag attgggttcg
      421 cacaaaggca cgaaggcacg aaggatccgc gaagcggctt aacccaccgc cgctccgaag
      481 acgtcgcagg gcctgcgctt gctggaccct ttgtgccttc gtgcctttgt gcgaaccaat
      541 attgcggcct tcggccgcgg cacggtggat gaccgttggg ctcgaaaatt cgaggataaa
      601 ggcgtcgacg gattgcttcg tcgcttcgct cctcgcaatg acggggaatg aacgagacga
      661 tcctgcgctt cggttatccc gcgaccctga tcgccgaata tgagcattgg gtggtgcttc
      721 ttcggccggc ccagccgacg ctgggaagcc tggtcctggc ggcgaagggc gaggcgacga
      781 gtttcgcgga cctccggccg caagccttcg gcgagcttca gcgggcggtg gcggacgtcg
      841 agcgggcgct cggcgccgcg atccgcttcg agaagatcaa ctatctgatg cttatgatgg
      901 tggacccgca cgtgcacttc catgtcatcc cccgctacga gggcgagcgc tcggcctgtg
      961 gggtgggcgt gaaggatgcg ggatggccca aggtgccggc gctgggcgag gcggtgacgc
     1021 tggagccgga gcaggtcgag gcgctggcgg ggtatttcag ggggtattgg ccggagtgat
     1081 gtaaatcctc cccggcattg gccggggagg gggaccgcgc gcagcgcggt ggaggggttc
     1141 tacggactta gcaagacccc tccaccatgc ttcgcatggt ccccctcccc cgcaattgcg
     1201 ggggaggatt tagaacgccc acctcgccgt cagcgccttc gcggcctcga cgtcttcggg
     1261 gaaatcgacc tcctgccatt cctcgccctt gatcgagaca atgctcacgt cggcgcccgc
     1321 caaagcgagc gagtcgatgg cgcgcagata ccagcggcgg gtgccgtcgg gctggcgcat
     1381 catccgctcg acctggtcgg cgaacaggcg cgggccttcg ccgcggaagg cgaggaggcc
     1441 gatcgattcg gcattatact ggccggcctc gagcgccttg ccaatgcgaa gcagccggtc
     1501 gccggagcgg agcaccttca tgtcgtcggc gtcgtagctc tccttctcgt cgacggtgac
     1561 ggcgatcggg ccgggagccg cttcgaggac cctctcgagc aggcggggcg agatcagggt
     1621 gtcgccgttg aggagcaggg tgtcgcggtc gagctcggct cgcgccatcc agaccgagcc
     1681 gagattgtcg gcgacatgat agaagggatt gaacagggtc cgcactccgg agcggcgcgc
     1741 cgtcacttcg gcgaccgtct cctcggcgaa gccggtgacg acgacgatgt cggcgacgcc
     1801 ggcgccctgg agcgcgtcga gctgccattc cagaaggctt cggccgttga ggtcgatcag
     1861 gcatttcggc ctcgtcgcgg tcagcggcag caggcgcgag cccttgccgg cgctgaggac
     1921 gatcgctttg ccgatggtca tgagaaaagc cgcattccca acgccgcggc ccgttgcgcg
     1981 acactttcga aacgggcctg ttatgtttcg acttgggcaa attcagggcg gcctttcgcc
     2041 tttcggcctt cttcccgcag tcgcc
//
LOCUS       DASWNU010000170         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_395202, whole genome shotgun sequence.
ACCESSION   DASWNU010000170 DASWNU010000000
VERSION     DASWNU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395202"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1145
                     /locus_tag="VGB57_03330"
     CDS             63..1145
                     /locus_tag="VGB57_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0130412.1"
                     /translation="MSSSAEQEPARGNRRTGKPTINDVARLSGVSKKTVSRVINRSPL
                     LNDETRDRVLGVIRDLGYVPNPQARALALRTNFLIGLIHDNPNAQMVLNVQQGILEAL
                     QGTDFAMVVRPVDRGSSTLVDDARHFLERQRPYGVVILPPLSENDAIARLCDELGCRY
                     VRMGSAELDDPDHMVVSNDREAVADAVQYLVTQGHRMIGLIAGPHGFRSAAERRLGFE
                     DALAAAGIRLPRSLIAEGNYTFESGIAAAEKLLDLSPRPTAIFASNDEMAAGVVHAAR
                     QRDISVPDDLSVVGFDDTPIAGHIWPPLTTVRWPIVSMARAAALKLLASEGDPDDEVS
                     EPSLFLSRLVKRASVAPPPGRPLSQS"
     gene            1420..2856
                     /gene="uxaC"
                     /locus_tag="VGB57_03335"
     CDS             1420..2856
                     /gene="uxaC"
                     /locus_tag="VGB57_03335"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEY0130413.1"
                     /translation="MPRPLKLDPDRLFPADPSSRSIARALYGEVAALPIVSPHGHTDP
                     AWFATDTPWSNPTELLLAPDHYLYRMLYSQGVPLDALGVPSRSGPSPADPREAWRLFA
                     SHYHLFRGTPSRLWLDQVFADVFGLDVALEAATADHYYDRLTEALATPAFRPRALFDR
                     FDIELLATTEGPHEDLVHHAAIRRSGWAGRVVTTYRPDAVIDPEHEDFAAALAAFAEL
                     SREDVHSWTGYLAAHRKRRSDFRAAGATATDHGHPTARTADLSRPEKEALFGRIVDGS
                     WSPRDAETFRAQMLTEMAAMSLDDGMVMQIHPGSFRNHNRWLHGAFGRDKGADIPTRT
                     DYVQALKPLLDRFGNEAGLSIILFTLDESAYARELAPLAGHYPCLKLGPAWWFHDSPE
                     GMRRFREMTTETAGFYNTVGFNDDTRAFLSIPARHDVARRVDCAFLARLVAEGRLQDW
                     EAAELAHELTYGLVKRAYRLDAEQAVAA"
BASE COUNT          452 a         1074 c          894 g          455 t
ORIGIN      
        1 caagggtgcg ccttggcttt cggcggatgc ggtgcgtata gcgaaagatc agggagagta
       61 gcttgagcag cagtgccgaa caggagcccg cccggggcaa ccgccgcacg gggaagccga
      121 cgatcaacga cgtcgcccgg ctgtcgggcg tctccaagaa gacggtgagc cgggtcatca
      181 accgctcgcc tctgctcaac gacgagaccc gcgaccgggt gctcggggtg atccgcgacc
      241 tcggctacgt gcccaatccg caggcccggg cgctggcgct tcgaaccaac ttcctgatcg
      301 gcctcatcca cgacaatccc aacgcccaga tggtgctcaa cgtccagcag ggcatattgg
      361 aagcgcttca gggcaccgat ttcgcgatgg tcgtccgccc cgtcgatcgc ggctcctcca
      421 ccctggtcga cgacgcccgc cactttctgg agcgccagcg tccctatgga gtggtcatcc
      481 ttccgccgct gtcggagaat gacgcgatcg cgcggctctg cgacgagctc ggctgccgct
      541 acgtccggat ggggtcggcc gagctcgacg acccggacca tatggtcgtc tccaacgacc
      601 gggaagcggt cgccgacgcc gtccagtatc tggtcaccca gggccatcga atgatcggcc
      661 tcatcgccgg tccgcacggc ttccgctccg ccgccgagcg ccgcctgggc ttcgaggatg
      721 cactcgccgc cgccggcata cggcttcccc gaagcctcat cgctgaaggc aattacacgt
      781 tcgaatcggg catcgccgcc gccgagaagc tgctcgatct gtcgccgcgg ccaaccgcga
      841 tcttcgccag caacgacgag atggcggccg gcgtcgtcca tgccgcgcgc cagcgcgaca
      901 tctcggtgcc cgacgacctg tccgtggtcg gcttcgacga caccccgatc gccggccata
      961 tctggccgcc gctgaccacg gtgcgctggc cgatcgtctc catggcccga gccgcggcgc
     1021 tcaagctgct cgccagcgaa ggcgatccgg acgacgaggt ttcggagccg tcgctcttcc
     1081 tgtcgagact cgtgaagaga gcgtcggtgg cgccgccgcc ggggcgcccg ctctcccaat
     1141 cctgaggcat ggctacgtcc cgctgaaatc ctccccatcg caagatgggg agggggacca
     1201 ggcgaagcct ggtggagggg taactggctc gggcatgtgc cgtcacgcgc cggaactccg
     1261 gaagcttctg ccatctaccc ctccaccaca cttcatgtgg tccccctccc caccgcttcg
     1321 cggcagggag gacaaaggtt gcaatcgatt atgacaccgg ttaccgctaa tattgcgcag
     1381 ccggcggaga ccggcgccgg cacaagcgtt ggagagcgaa tgccacggcc cctgaagctc
     1441 gatccggaca ggctgttccc ggccgatccg agcagccgct cgatcgcgcg tgccctctat
     1501 ggcgaggtcg cggcgcttcc gatcgtgagc ccgcacggcc ataccgatcc cgcctggttc
     1561 gcgaccgata cgccgtggag caaccccacc gagctcctcc tcgcgccgga ccattatctc
     1621 tatcggatgc tttatagcca gggcgtgccg ctggacgcgc tcggggttcc aagccgctcc
     1681 ggcccctccc ccgccgaccc gcgcgaggcc tggcggctgt tcgcgagcca ttatcacctg
     1741 ttccgcggca cgccctcccg gctgtggctg gaccaggtct tcgcggacgt gttcgggctc
     1801 gacgtcgctc tggaagcggc caccgccgac cattattacg accgcctgac cgaagcgctc
     1861 gccacgcccg cattccgccc gcgcgcgctt ttcgaccggt tcgacatcga gcttctcgcg
     1921 accaccgagg ggccgcacga ggatctcgtc caccacgccg ccatccgccg gtcgggctgg
     1981 gccggccggg tcgtcaccac ctaccgtccg gacgcggtga tcgatcccga gcatgaggat
     2041 ttcgccgccg ccctcgcggc cttcgccgag ctgtcgcggg aggacgtgca cagctggacc
     2101 ggctacctcg ccgcgcaccg caagaggcgc tccgacttcc gcgccgcggg cgccaccgcc
     2161 accgaccacg gccacccgac cgcgcgcacc gccgacctct cgcggcccga gaaggaagcc
     2221 ttgttcggcc ggatcgtcga cggcagctgg tcgccccgcg acgccgagac cttccgagcc
     2281 cagatgctca ccgagatggc cgcgatgagc ctcgatgacg ggatggtgat gcagatccac
     2341 ccgggctcgt tccgtaacca caaccgctgg ctgcacggcg ccttcggccg cgacaagggc
     2401 gccgatatcc cgacccggac cgattacgtc caggccctga aaccgctgct cgaccgcttc
     2461 ggcaacgagg ccggcctgtc gatcatcctc ttcaccctgg acgaaagcgc ctatgcccgc
     2521 gagctcgccc cgctggccgg ccattatccg tgcctgaagc ttggccccgc ctggtggttc
     2581 cacgattcgc cggaaggcat gcgccgcttc cgcgagatga cgacggagac ggccggcttc
     2641 tacaacacag tcggcttcaa cgacgacacc cgcgccttcc tttccatccc cgcgcgccac
     2701 gacgtcgccc gccgggtcga ctgcgccttc ctggcccggc tggtggcgga aggcaggctt
     2761 caggactggg aagccgccga gctggcgcat gagctgacct acggcctcgt caagcgcgcc
     2821 taccgcctcg acgccgagca ggcggtggcc gcgtgatttc cctcttcccc gtcat
//
LOCUS       DASWNU010000171         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_84198, whole genome shotgun sequence.
ACCESSION   DASWNU010000171 DASWNU010000000
VERSION     DASWNU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84198"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="VGB57_03340"
     CDS             complement(<1..2004)
                     /locus_tag="VGB57_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0130414.1"
                     /translation="MSKFKFRETLLASTMIAGMSFANPAFAQDANDPAGTTPPPGSTG
                     APAPSDEDTPTDGVQPAAGQDAAEETSAGDIVVTGTLIRNPNLVSSSPVSVVGQEELQ
                     LRQTNVAEEILRDLPGVAPSIGSAVNNGNGGAAYADLRGLGNFRNLVLLDGVRIVPSN
                     FIGRVDLNNIPLALVERVDTLTGGAATTYGADAVSGVINFITRSDFAGMELSASNQIT
                     QRGDGHIFRVDATVGANFDDGRGNAVLSIGYQEADPVYQGARRFSLNNYTSTSGNAGG
                     SGTTVPARFTRPVDPVFQPGVLQNTIVPTLGITRGYVGSTDAFNFNPYNIFQTPFKRF
                     NIFGSGHYEVADNVEFYTRGMFSKNTVNTIVAPSGIFGQLALIPVSSPFLPAGARAQF
                     CQSNDFDFTTPGIQRLTPAQCAAAALATSPSDPNFRAFQTNIGRRIVEAGPRISEYTT
                     TMFDYRAGVRIGITDTIGLDVAGAYGESENEQEQQGYTSISRLRTALYSTSTTTCNLG
                     PSPTVPNPTNANLPPLAGAGSGTQGCVPVNIFGPAGSISPEAIDFITATAFNSQESSL
                     AQARALLSGDFGASAPWAEDPIGFAVGTEYRNYQSRQTADQLSQTAGELGGAGGAVLP
                     FEGGYDVKEVYAEIIAPIVQNRPFFETLTLEAGIRYSDYKIDAA"
     gene            2212..2745
                     /locus_tag="VGB57_03345"
     CDS             2212..2745
                     /locus_tag="VGB57_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130415.1"
                     /translation="MKLGSRIVAIVALAAVVPAPAPAKPKLQDARSNAFIQALSACRG
                     VTDEKARLDCYDRASARLAEAVDKKELVVMDRQEIRETRRSLFGFSVPNIPLFRGESG
                     GDDGKLETVIAGASRLEGGRWQIRLEDGAVWHTNETRLNLSDPRPGQKIVIQRGTLGN
                     YFLRIDGQRGVRGRRVS"
     gene            complement(2870..3070)
                     /locus_tag="VGB57_03350"
     CDS             complement(2870..3070)
                     /locus_tag="VGB57_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEY0130416.1"
                     /translation="MLMSNKGREARLHYMPGTFRILTPGDHVRCAATGQPIPLDELRY
                     WSVARQEAYSSPELSVRAEGRG"
BASE COUNT          524 a          943 c         1127 g          519 t
ORIGIN      
        1 ggctgcgtcg atcttgtagt ccgaatagcg gatgcccgct tcgagcgtca gggtctcgaa
       61 gaagggccgg ttctggacga tcggagcgat gatctcggca tagacttcct tgacgtcata
      121 gccgccttcg aacggaagaa ccgctccgcc cgcgccgcca agctcacccg ccgtctgcga
      181 cagctggtcg gcggtctgcc gggactggta attccggtac tcggtgccga cggcgaagcc
      241 gatcgggtcc tccgcccagg gcgcggaagc tccgaagtcg ccgctcagaa gcgccctcgc
      301 ctgggccagc gaggattcct gcgagttgaa cgcggtcgcc gtgatgaagt cgatcgcctc
      361 gggcgagatc gacccggcgg ggccgaagat gttgaccgga acgcagccct gggttcccga
      421 acccgcgccg gccagcggcg gcaggttcgc gttggtcggg ttcgggacgg tcggcgacgg
      481 ccccaggtta caggtcgtcg tgctggtcga gtagagcgcc gtgcgaagcc tcgagatcga
      541 ggtatagccc tgctgctcct gctcgttctc ggactcgccg taggcgccgg cgacgtcgag
      601 accgatcgtg tccgtgattc cgatccggac gccggcgcgg tagtcgaaca tcgtcgtcgt
      661 atattcggag atacggggtc cggcctcgac gatacggcga ccgatattgg tctggaaggc
      721 gcggaagttc ggatcgctcg ggctggtcgc cagggccgcc gccgcgcact gcgcgggcgt
      781 gagcctctgg atgcccgggg tggtgaaatc gaagtcgttg ctctggcaga actgagcgcg
      841 ggcaccggcc ggaaggaacg ggctgctgac cgggatcaga gccagctggc cgaagatgcc
      901 cgacggagcg acgatcgtgt tgaccgtgtt cttggagaac atgccgcggg tgtagaattc
      961 aacgttgtcg gcaacctcgt aatggcccga gccgaagatg ttgaagcgct taaacggcgt
     1021 ctggaagatg ttgtaggggt tgaagttgaa ggcgtcggtc gagccgacat agccccgggt
     1081 gatgcccagg gtcggaacga tcgtgttctg gaggacgccc ggctggaaaa cggggtccac
     1141 cggccgggtg aagcgggccg ggacggtcgt gccggaaccg ccggcattgc ccgacgtcga
     1201 cgtatagttg ttgagcgaga agcggcgagc gccctgatag acggggtccg cttcctggta
     1261 gccgatcgag aggaccgcgt taccgcgacc gtcgtcgaaa ttggctccga ccgtcgcgtc
     1321 gacgcggaag atgtggccgt cgccgcgctg agtgatctgg ttgctggcgg aaagctccat
     1381 tccggcgaag tcgctgcggg tgatgaagtt gatgacgccc gacacggcgt cggcgccgta
     1441 ggtcgtggcg gcgccgccgg tcagggtgtc gacccgctcg acgagcgcga gcgggatgtt
     1501 gttcagatcg acgcggccga tgaagttcga cggaacgatg cggacgccgt cgagaaggac
     1561 gaggttgcgg aagttgccga ggccgcgaag atcggcgtag gccgcgccgc cgttgccgtt
     1621 gttgacggcg gagccgatgc tcggtgcgac gcccggaagg tcgcgaagga tctcttcggc
     1681 gacgttggtc tggcggagct ggagctcttc ctggccgacc accgagaccg gcgaagacga
     1741 gaccaggttc ggattgcgga tcagggtgcc ggtgacgacg atgtcgccgg cgctggtctc
     1801 ttcggccgcg tcctgtccgg ccgcgggctg gacgccgtcg gtcggcgtgt cctcgtccga
     1861 cggggccgga gcgccggtcg acccgggcgg aggcgtggtg cccgcgggat cgttggcgtc
     1921 ctgagcgaag gccggattgg cgaagctcat gcccgcgatc atggtcgagg caaggagcgt
     1981 ttccctgaac ttgaatttcg acatttgcgt ccccttggtg ttcaataaag gtgcaccggg
     2041 cgcagtttcg ccccccaccc atccgatacc tgtttatgtc gagggtgtaa cggcacgcaa
     2101 gcagggccac cgtcggcgga acattcttgg cacggcctgt aacttttcag ccacagtctt
     2161 gccgcgacct cgcttagccc ataacctcgc cagaggcgga ggggcggaat catgaagctt
     2221 ggcagcagga tcgtcgcgat cgtggcgctg gcggcggtgg tgcccgcccc ggccccggcc
     2281 aagccgaagc ttcaggacgc gcgcagcaac gccttcatcc aggcgctgtc ggcctgccgc
     2341 ggagtgaccg acgagaaagc caggctcgac tgctacgaca gggcgagcgc ccgcctggcc
     2401 gaggcggtgg acaagaagga gctggtcgtc atggaccggc aggagatacg ggagacccgc
     2461 cgctccctgt tcggcttcag cgtcccgaac atccccctgt tccggggcga aagcggcggc
     2521 gacgacggca agctcgagac ggtgatcgcc ggcgccagcc ggctggaagg cggcagatgg
     2581 cagataaggc tcgaggacgg agccgtctgg cacaccaacg agacccgcct gaacctttcc
     2641 gatccccgcc ccggccagaa gatcgtcatc cagcgcggca ccctcggcaa ttacttcctg
     2701 cgcatcgacg ggcagcgggg agtgcgggga cggcgggtca gctaggcggg gagagggacg
     2761 aaggccgtgg ccgtgagctg gtttcacggt ccggttctcc gccagcttgg cggctcgccc
     2821 ataggtgtcg aaacaagttc agcatgaccg gaagggacga aatccgcgcc tacccccgcc
     2881 cctccgctct caccgaaagc tcaggcgagg aataagcttc ctgccgggcc acgctccagt
     2941 aacgaagctc gtccagcggg atgggctggc cggtggcggc gcagcggacg tggtcgccgg
     3001 gggtgaggat gcggaaggtc cccggcatgt agtggagccg cgcttcgcgg cccttgttcg
     3061 acatcagcat cagggcctcg ctagagcaat ttgggctgct cgcccgtggg ctt
//
LOCUS       DASWNU010000172         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_110731, whole genome shotgun sequence.
ACCESSION   DASWNU010000172 DASWNU010000000
VERSION     DASWNU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110731"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VGB57_03355"
     CDS             complement(<1..530)
                     /locus_tag="VGB57_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0130417.1"
                     /translation="MNEAAALPRAREAEVFAELARGLCVVNLAKSYDRKRVLKDVSLH
                     VDRGEVVGLLGPNGAGKTTCFYSVMGLVKPDSGRIMLDGQDITGLPMYRRAVLGLGYL
                     PQETSIFRGLTVAQNIMAVLEVSERDKAVRRERLEQLLGEFHIEHLRDAPAMALSGGE
                     RRRCEIARALAAHPSI"
     gene            complement(702..1220)
                     /locus_tag="VGB57_03360"
     CDS             complement(702..1220)
                     /locus_tag="VGB57_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY0130418.1"
                     /translation="MKRLLPLLLLAALGPAAAFGQVSALKGHDSNAPVDVAADRIEVQ
                     DRADRAIFSGNVVVRQGQLTLTAPRINVAYSSGGGVQLRRIDATGGVTVKSPSETARG
                     NVGIYDTQARIITLIGDVSLVQRDANVNGGRLTIDLDSGRAVMDGGGPPGTTSQGGRV
                     TGRFTVPQKKGG"
     gene            complement(1226..1864)
                     /gene="lptC"
                     /locus_tag="VGB57_03365"
     CDS             complement(1226..1864)
                     /gene="lptC"
                     /locus_tag="VGB57_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY0130419.1"
                     /translation="MSELADDRRAAKRGWAAPGGLHDWLMRFLKIALPAVVGLLLAYL
                     LLSPLSKEKEISFLLDKNKVDTARERLKTQKAEYRGLDDKGRAFSIQAAQAVQEKSSV
                     PVVDIGGMSARIQLEEGPATITADKARYNMDTQKVDVLGPILFTASDGYRLETRDVAL
                     DLNKHVAVSGGGVEGQMRLGRFTAGGMTVDLPQRKVVLTGRPRLHIVQGAIR"
     gene            complement(1857..2474)
                     /locus_tag="VGB57_03370"
     CDS             complement(1857..2474)
                     /locus_tag="VGB57_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY0130420.1"
                     /translation="MTVYFHEEDLPEGVLAPGPVAVDTEAMGLLPGRDRLCLVQISDG
                     GGDEHLVRFNPGSDYSAPVLKAVLADPERLKLYHFARFDLAIMQAYLGVMAGPVYCTK
                     TASRLIRTYTDRHGLKELIKELLQIDISKQQQTSDWGAPELSDAQKDYAASDVRYLHQ
                     MKEKLDERLEREGRTKLARACFDFLPHRALLDLAGWAEVDIFAHV"
     gene            2700..3632
                     /locus_tag="VGB57_03375"
     CDS             2700..3632
                     /locus_tag="VGB57_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEY0130421.1"
                     /translation="MPSGLAALLDDVAAIAKLAAASIDDVGAAAGRAGAKAAGVVIDD
                     TAVTPRYVTGFTPDRELPIIARIAKGSLKNKLLILLPAALALSALLPWAITPLLMLGA
                     AYLCFEGAEKVWEAVSEPKHSLAEDAAELSSAEHEQKMVGGAVRTDFILSAEIMAIAL
                     AEVASEPFWTRAIILAVVAIAITIGVYGLVGLIVKMDDIGLHLAERRNRGTRALGRGL
                     VLAMPKVMSALSTIGIAAMIWVGGQIIVHGLEVFGFAGPAHLIHDAGAAAGHAVPALA
                     GAIEWTVGAAGSGLIGLALGALIVFVHHKVARKH"
BASE COUNT          630 a         1289 c         1396 g          649 t
ORIGIN      
        1 atgatcgagg gatgggcggc gagcgctcgg gcgatctcgc agcggcggcg ctcgcctccg
       61 gacagcgcca ttgcgggggc gtcgcgcaga tgctcgatgt ggaactcgcc gagcaattgc
      121 tcgagccgct cgcgccggac cgccttgtcc cgctccgaca cttcgagcac cgccatgatg
      181 ttctgggcga cggtgaggcc gcggaagatc gacgtctcct gcggcagata gccgaggccg
      241 agcacggcgc ggcggtacat cgggaggccg gtaatgtcct ggccgtccag cattatccgc
      301 ccgctgtcgg gcttcaccag gcccatcacc gaatagaagc aggtcgtctt gccggcgccg
      361 ttggggccga gcagaccgac cacctcgccc cggtcgacgt ggagcgagac gtccttcagc
      421 acgcgcttgc gatcgtagga tttggcgagg ttgacgacgc acagcccgcg cgccagctcg
      481 gcgaacacct ctgcctcgcg ggctctggga agggcggcgg cttcgttcat caggtccgca
      541 ggcttaagcc atgcggctta aggggtaaac tggcaggctt cgtgcatgcg cgaaaaaaaa
      601 ggcgggggag cggtccgtca tcccggcgaa agccgggacc tcgtcgagga aaaggctcga
      661 cgggtctccc cgaggtcccg gcttccgccg atgacgggtg cttacccgcc cttcttctgc
      721 ggcaccgtga acctgccggt gacccggccg ccctggctgg tcgttccggg cggcccaccg
      781 ccgtccatga cggcgcggcc gctgtcgaga tcgatggtga gccggccgcc gttcacgttc
      841 gcgtctcgct ggaccaggct gacgtcgccg atcagcgtga taattcgcgc ctgggtgtcg
      901 tagatcccca cgttcccgcg cgccgtttcc gacggactct tgacagtgac tccgccggtg
      961 gcgtcgatcc ggcgcaactg cacgccgccg ccactggaat aagcgacgtt gatgcggggc
     1021 gcggtgaggg taagctggcc ctgtcggacg acgacgttgc ccgagaagat cgcccggtcg
     1081 gcccggtcct gaacttcgat ccggtccgcg gcgacgtcga ccggggcgtt ggaatcgtgc
     1141 cccttcagcg cggaaacctg gccgaacgcg gccgccgggc cgagcgcggc cagcagcagc
     1201 agtggaagta ggcgcttcat gcccttcatc tgatagcccc ttgcacaata tgcaagcgcg
     1261 gacggccggt cagcaccacc ttgcgctggg gcaggtccac cgtcatcccg ccggcggtga
     1321 accggcccag ccgcatctgg ccctcgactc cgcccccact gacggcgaca tgcttgttga
     1381 ggtcgagcgc cacgtccctt gtctcgagcc ggtagccgtc cgaggcggtg aacaggatgg
     1441 gtccgagcac atcgaccttc tgcgtgtcca tgttgtagcg cgccttgtcg gcggtgatcg
     1501 tggcggggcc ttcctcgagc tggatccgag ccgacatccc gccgatatcg accaccggaa
     1561 cgctcgattt ctcctggacg gcctgcgccg cctggatcga gaaggcccgc cccttgtcgt
     1621 cgaggccgcg atattccgcc ttctgggtct tcagccgctc gcgggccgtg tcgaccttgt
     1681 tcttgtcgag caggaagctg atctccttct ccttcgagag aggcgacaga agaagatagg
     1741 cgagaagcag gcccacgacc gccgggagag cgatcttgag gaaccgcatc agccagtcgt
     1801 gaaggcctcc cggggccgcc cagccgcgct tggcggcgcg ccggtcgtcc gcaagctcag
     1861 acatgggcga agatgtcgac ctcggcccag ccggcgagat cgagcagggc ccggtgcgga
     1921 aggaaatcga agcaggcccg ggccagcttc gttcggcctt cgcgctccag ccgctcgtcg
     1981 agcttctcct tcatctggtg aaggtagcgg acgtcggagg cggcataatc cttctgggcg
     2041 tcggaaagtt cgggggcgcc ccagtccgac gtctgctgct gcttggagat gtcgatctgg
     2101 agaagctcct tgatcagctc cttgagcccg tggcggtccg tataggtccg gatcaggcgc
     2161 gaggcggtct tggtgcaata gacggggccg gccatcaccc cgagataggc ctgcatgatg
     2221 gcaagatcga agcgcgcgaa atgatagagc ttgagccgct cgggatcggc gagcacggcc
     2281 ttgagaacgg gcgctgaata gtcgctcccg gggttgaagc ggacgagatg ctcgtcgccg
     2341 ccgccgtcgg agatctggac gaggcagagc cggtcgcggc cgggcaggag ccccatcgcc
     2401 tcggtgtcga cggcgacggg gccgggggcg agcacgccct cgggaagatc ctcttcgtgg
     2461 aaatatactg tcatggcctc cgcataggcc gaagcgcctt cacgctcaat caccggcagc
     2521 gcggcgatga agcggaaccc attcgtcatg ccagcgaagg ctggcatccc ggcgggtgaa
     2581 gaacgattgc gcctttcacc cctggggcgc cagcctgcgc tgggcgacgg cgtgtgaccg
     2641 gacttgatcg ttgctgtccg ccccgccaca ggagcgcgaa cagccaggag ccccgcccca
     2701 tgccttccgg tctcgccgca ctgctcgacg acgtcgccgc catcgcaaag ctggcggccg
     2761 cctcgatcga cgatgtcggc gcggccgccg gccgcgcggg cgccaaggcg gcgggggtgg
     2821 tgatcgacga tacggcggtg accccgcgct acgtcaccgg cttcacgccg gatcgcgagc
     2881 ttccgatcat cgcccggatc gccaaggggt cgcttaagaa caagctcctc atcctcctgc
     2941 cggcggccct ggcgctgagc gcgcttctgc cctgggcgat cacgccgctg ctcatgctcg
     3001 gcgccgccta tctctgcttc gaaggcgccg aaaaggtttg ggaagccgtc agcgagccga
     3061 agcacagcct ggccgaggac gccgccgagc tgagcagtgc ggagcatgag cagaagatgg
     3121 tgggcggagc ggttcggacc gacttcatcc tgtcggccga gatcatggcc atcgccctcg
     3181 cagaggtggc gtcggagccg ttctggaccc gggcgatcat cctggccgtg gtcgcgatcg
     3241 cgatcacgat cggagtctac ggtctcgtgg gcctgatcgt gaagatggac gatatcggcc
     3301 tccatctcgc cgagcggcgc aaccggggca cgcgggcgct tgggcggggc ctcgtcctcg
     3361 ccatgcccaa ggtgatgtcg gctctgtcca cgatcgggat cgcggcgatg atctgggtcg
     3421 gcggccagat catcgtccac ggactggaag tcttcggttt tgccggcccg gcccatctga
     3481 tccacgatgc cggcgccgcg gcgggccacg ccgtgccggc cctggccgga gcgatcgaat
     3541 ggacggtcgg agcggcaggc tccggtctca tcggactcgc gctcggggcc ctgatcgtct
     3601 tcgtccatca caaggtcgcc cggaagcatt gacggccgcg cccgaagccc tgtggaaaag
     3661 cgctcgcggc tgggaatgat ttgagctata tgagtcggcg gtgcgccaat ttcgggggcg
     3721 cacgagaccg ttggcgcgac agccccgcgc ggtggtttgc aaatgaaggc aggggcgcgg
     3781 aaagtattga cgggcatggg tttcgacaga gggcgccgcg gtgagcgcgg cgggcgcggc
     3841 agagacaagc gcgacggatt cggcgacgag aatttcagcg gatttgaaga gcgcggcggc
     3901 ggcggcggcg accgattcgg cggcggcggc ggcggcggtt acggcggtgg cggcggcggg
     3961 ggcg
//
LOCUS       DASWNU010000173         6003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_343551, whole genome shotgun sequence.
ACCESSION   DASWNU010000173 DASWNU010000000
VERSION     DASWNU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343551"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="trmD"
                     /locus_tag="VGB57_03380"
     CDS             complement(<1..705)
                     /gene="trmD"
                     /locus_tag="VGB57_03380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY0130422.1"
                     /translation="MTWSATVLTLYPEMFPGPLGVSLAGRALEQEIWSLEAVQIRDFA
                     TDKHRSVDDTPAGGGAGMVMRADVLGAAVDHVLTHTSKAPFLAMTPRGRPLTQAFVRK
                     IAAGPGVSILCGRFEGIDERLFEARPIRQVSVGDYVLSGGETGAIVLLDACVRLLPGV
                     MGAASSGDEESFESGLLEYPHYTRPQMWEGRTIPEVLRSGDHARIAAWRQAIAESDTR
                     SRRPDLVGSHRDSRRPP"
     gene            complement(702..1088)
                     /locus_tag="VGB57_03385"
     CDS             complement(702..1088)
                     /locus_tag="VGB57_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0130423.1"
                     /translation="MTGPQYLLDTNCCIYLLSRTRPVLEAKLAKCPSGSVGLSAIVAA
                     ELILGFSNASSKLKAMLERFLREFPVQPFDEEAARAYAQVPFRRGKLDRLVAAHALSL
                     DATLITNNVRDFADVPDLKLQNWTQL"
     gene            complement(1085..1351)
                     /locus_tag="VGB57_03390"
     CDS             complement(1085..1351)
                     /locus_tag="VGB57_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0130424.1"
                     /translation="MKKTKTFKSGNSVAVRLPKALGAEPGVEMTVREERGRYIVEPVE
                     EPTDGKKIDLTGIYGSMRSVSIRLDPEEYEDIPREWHLLGLDLE"
     gene            1437..2390
                     /locus_tag="VGB57_03395"
     CDS             1437..2390
                     /locus_tag="VGB57_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0130425.1"
                     /translation="MTEPTFDCLIVGGGPAGLTAAIYLTRFHLTAKLVDAGKSRAGWI
                     PCTHNHAGYPDGISGKELIARMKAQAQKYGTSIVTSRVTRLDVVEGGFKAEWGEGSVI
                     ARKVLIATGVTNRRPPMDEDLHDEALARGLIRYCPICDGYVVTDKKVGVIGSGSHGIA
                     EAVFLRGFTEDITLIAPHAAHDLSAEDQARLKEYGIQTLDGPCSAVAAHEDCIVVDTA
                     DGHHAFDSVYPALGSDMHNELAAQVGAELKDGNIVVDSHQRTSVPGLYAAGDIVLGLD
                     QISHAMGEGGVAAVTIRNDLAKERQIRREKLSGSIAAKSSA"
     gene            complement(2474..2956)
                     /gene="rimM"
                     /locus_tag="VGB57_03400"
     CDS             complement(2474..2956)
                     /gene="rimM"
                     /locus_tag="VGB57_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456920.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY0130426.1"
                     /translation="MPEDQVALAAIAGAHGISGEVRLKLFAESADSLKRHKQVRVGER
                     LLTLASLKGDRTPIARFAEIPDRTAAEALRGQLVTVPRSALPPLEEGEYYHADLIGLP
                     CVSPAGEPLGRVVAVEDFGAGDILEIEKPDGRRTMVPFRAGVADLIDGRIVADPEFLA
                     "
     gene            complement(3113..3589)
                     /gene="rpsP"
                     /locus_tag="VGB57_03405"
                     /pseudo
     CDS             complement(3113..3589)
                     /gene="rpsP"
                     /locus_tag="VGB57_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(3695..5170)
                     /gene="ffh"
                     /locus_tag="VGB57_03410"
     CDS             complement(3695..5170)
                     /gene="ffh"
                     /locus_tag="VGB57_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY0130427.1"
                     /translation="MFDSLSDRLGAVFDRLRGHGALNEADVRSAMREVRIALLEADVA
                     LPVVREFVDKATEQAVGQQVLRSITPGQQVVKIVHDALVEMLGAEASDLNVDVTPPAV
                     VMMVGLQGSGKTTSTAKIARRLREKAGKKVMMASLDVARPAAQEQLRVLGEKAEVATL
                     PIVAGQQPVDIARRALQAARLQGYDVLLLDTAGRLHVDQALMDEMKAVADVSSPAEIL
                     LVVDALTGQDAVNVASSFSGQVPLTGVVLTRLDGDARGGAALSMRAVTGKPIKFAGVG
                     EGIDALEAFHPERVAGRILGMGDVVSLVERAQETIQVEEAEAMARKMAKGLFDLDDLR
                     SQLAQMQRMGGISGLAAMLPGMKKISGAMEASGVDNKVLVHLDAIIGSMTPKERKRPE
                     LLNAKRKIRVAKGSGRTVQEVNKLLKMHQDMASAMKKIRKMGGLGKLGALFGGGGLGG
                     MGGLGGMPGLPGGGAGGMPGLPGPGGAPMNLPPGFEKFMKK"
     gene            complement(5342..>6003)
                     /locus_tag="VGB57_03415"
     CDS             complement(5342..>6003)
                     /locus_tag="VGB57_03415"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130428.1"
                     /translation="LRGDYMIDFNDPAHDGALRNLENLTLSSAGDERYARGGAGFDYD
                     VTWADALLGAGGTITINGALLRSNETMDFDGSLETDGSFRIFAGAGDDSLSGAAGADL
                     IAGGLGGDTLRGGGGNDVFRYDGAADSSPSATDLIEDFSTGDRIDLGRIDADAGLAGD
                     QAFSFIGSAAFGNHAGELRYENVAGTTWLVQGDTDGDGSVDLELLLVAAGNPVAADFI
                     L"
BASE COUNT          934 a         2047 c         2023 g          999 t
ORIGIN      
        1 cggtggcctt cgagagtccc tgtgactccc gaccaggtcc ggccgccttg accgtgtgtc
       61 ggactccgct atggcttggc gccaggcggc tatcctcgca tgatcccccg atcgcagcac
      121 ttcggggatc gtgcgccctt cccacatttg cggtcgggtg tagtgcggat attcgaggag
      181 gccgctttcg aagctttcct cgtccccgct ggaagccgcg cccattactc cgggaagcag
      241 ccgaacgcaa gcatcgagca gaacgatcgc cccagtctcg ccgccggaaa gcacatagtc
      301 gccgaccgaa acctgccgga tcggccgcgc ctcgaacagg cgctcgtcga tgccctcgaa
      361 gcggccgcac aggatggaga ctccggggcc ggcggcgatc ttacggacga aggcctgggt
      421 gagggggcgg ccgcgggggg tcatggcgag gaagggcgcc ttggaggtat gcgtcaggac
      481 atgatcgacc gctgcgccaa gcacgtcggc gcgcattacc atccccgccc ctccccccgc
      541 cggggtgtcg tcgacggagc ggtgcttgtc cgtggcgaag tctcggatct gaacggcctc
      601 cagagaccag atttcctgct cgagcgcccg tccggcgagg ctcacgccga gcgggccggg
      661 aaacatctcg gggtagagcg tcaggacagt ggcgctccaa gtcataactg tgtccaattc
      721 tgcagcttca gatccggcac gtcggcgaag tcccgcacgt tattcgtgat caacgttgca
      781 tcgagggaca aggcatgagc tgccaccagc ctgtccagct tcccccggcg aaacggcacc
      841 tgcgcgtagg cacgggcagc ttcttcgtcg aatggctgga ccggaaactc ccgcaggaac
      901 cgttcgagca tcgccttaag ctttgagctc gcattgctga acccgaggat gagttcggcg
      961 gcaacgatcg ccgatagccc cactgacccg gacgggcact tggcgagctt cgcttcgagg
     1021 accgggcggg tgcgggagag aagatagatg cagcagttcg tatccaggag gtattgggga
     1081 cctgtcactc cagatcgagc cctaggagat gccattcccg aggaatatcc tcatactctt
     1141 ccgggtcgag acgtatcgag actgaacgca tcgagccgta gattcccgtc aggtcgattt
     1201 tcttgccgtc ggtcggttcc tcgacgggct cgacgatgta tctgccacgc tcctcacgca
     1261 cggtcatctc gactcctggc tccgcgccga gtgccttcgg gagtcgaacg gcaaccgaat
     1321 tgccggactt gaaggttttg gtttttttca tgcaagatcc gtatatactt cgagtatata
     1381 caacgctgcc catcacggca agcggaaccg ccttcgccgc cgagcgcttg gccgcgatga
     1441 ccgagcccac cttcgactgc ctgatcgtcg gcggcggccc cgccggcctg accgcggcca
     1501 tctacctcac ccgcttccac ctcaccgcca agctggtcga cgccggcaag agccgggcgg
     1561 ggtggatccc ctgcacccac aaccatgccg gctatcccga cggaatttcc ggcaaggagc
     1621 tgatcgcccg gatgaaggcg caggcgcaga aatacggcac ttcgatcgtc accagccggg
     1681 tgacccggct cgatgtggtc gagggcggct tcaaggcgga atggggcgaa ggctcggtga
     1741 tcgcgcgcaa ggtgctgatc gccacgggag tgaccaaccg gcgcccgccg atggacgagg
     1801 acctgcacga cgaggcgctc gcccgcggcc tcatccgcta ctgcccgatc tgcgacggct
     1861 atgtagtgac ggacaagaag gttggagtga tcgggagcgg gtcgcacggg atcgcggagg
     1921 ccgtgttcct gcgcgggttc acggaggaca tcacgctcat cgcaccccat gccgcccacg
     1981 atctgagcgc ggaggaccag gcccggctga aggaatatgg gatacagacc ctcgacggcc
     2041 cctgctccgc ggtcgccgcc catgaggact gcatcgtcgt cgacaccgcc gacggccacc
     2101 acgccttcga cagcgtctat cccgcgctcg gctccgacat gcacaacgaa ctcgccgccc
     2161 aggtcggggc cgagctcaag gacggcaaca tcgtcgtcga cagccaccag cgcaccagcg
     2221 tccccggcct ctacgccgcc ggcgatatcg tcctcgggct cgaccagatc agccacgcca
     2281 tgggcgaagg cggggtcgcc gcggtcacca tccgcaacga cctggccaag gagcggcaga
     2341 tcaggcgcga gaagctgagc ggctcgatcg cggccaagag ttcggcttag ccgtcatccc
     2401 ggcgaaagcc gggacctcgg gagacccgcc gagacttctc gttctgaggt cccggctttc
     2461 gccgggatga cggttatgcc aggaactccg gatcggcgac gatccggccg tcgatcaagt
     2521 ccgcgacgcc cgcgcggaac ggcaccatcg tccgcctgcc gtcgggcttc tcgatctcga
     2581 gaatgtcgcc agcgccgaaa tcctcgaccg cgaccactcg ccccagcggc tcgcccgccg
     2641 ggctgacgca aggaaggccg atcaggtcgg cgtgataata ttcgccctcc tcgagcggcg
     2701 gaagcgccga gcgtgggacg gtgacgagct ggccgcgcaa cgcttccgcg gcggtccggt
     2761 cggggatctc cgcgaaacgg gcgatcggag tgcggtcgcc cttgagcgag gcgagggtga
     2821 ggagtcgctc ccctaccctc acctgcttgt ggcgcttgag gctgtccgcg ctctcggcga
     2881 acagcttcag gcgcacctct ccgcttatgc cgtgcgctcc ggcgatggcg gcgagggcga
     2941 cctgatcttc cggcaaggct tattcggcct tttcggcgcc ctcggcggga gcggcttcgt
     3001 cggagccctc ggccggctcg ctggtcgaga cgccctcgtt ggtcgcctcg tcggcgggcg
     3061 tcgcttcacc ctcggcggcg ggagcctcct cggcggcggg cgcctcttca gcggccggag
     3121 tttcctcagc agccggagcc tcctcggcgg caggcgcttc ggcggcggcc tcctcggcga
     3181 cgacttcgtc ggcggccgga gcctcctcgg ccgcgggagc ttcctccttg gccgcggcgg
     3241 cggtcgcggc ctcggcttcg cgggtggcgc gctcctcggc gcgctccttg gccttgtcgc
     3301 cgggaacgcc cttgttcggg ttgctgcggg cggcgcgctc gcgcactccg gcggcgtcga
     3361 ggaaacgggc gacgcggtcg ctcggctggg ctccgacgga gagccagtga cgggcgcggt
     3421 cggagtcgag cttcacgcgc tccgggctgt ccttggggag gagcggattg tagcttccga
     3481 tccgctcgat gaacttgccg tcgcgcgcgg tgcgggcgtc ggcgacgacg atcttgtaat
     3541 aagggcgctt cttcgcgccg ccccgggaaa gccggattga aactgccatt gttggtcctt
     3601 ctcttctgat ctgtcgtcac cccggcggaa gccggggcct cagggagagt tctcgctgag
     3661 gtaccggctt tcgccggtat gacggtggtt gcgtctattt cttcatgaac ttttcgaagc
     3721 cgggcgggag gttcatcggc gctccgccgg ggcctgggag gccgggcatt ccgcccgccc
     3781 ccccgccggg aaggccgggc attccgccca acccgcccat tccgccgagg ccgccgcctc
     3841 cgaacagggc gccgagcttg ccgagaccgc ccatctttcg gatcttcttc atcgcgctgg
     3901 ccatgtcctg gtgcatcttg agaagcttgt tgacctcctg caccgtccgt ccggagccct
     3961 tggcgacccg gatcttgcgc ttggcgttga gcaattcggg cctcttgcgc tccttgggag
     4021 tcatcgaacc gatgatcgcg tcgagatgga cgagcacctt gttgtcgacg ccgctggctt
     4081 ccatcgcccc cgagatcttc ttcatcccgg gaagcatcgc ggcgagaccg gagatgccgc
     4141 ccatgcgctg catctgggcg agctgggacc gaaggtcgtc gaggtcgaac aggcccttgg
     4201 ccatcttccg ggccatcgcc tcggcttctt cgacctggat cgtctcctgg gcgcgctcaa
     4261 cgaggctgac gacgtcgccc atgccgagga tccggcccgc gacccgctcc gggtggaagg
     4321 cctcgagcgc gtcgatcccc tcgccgactc cggcgaactt gatcggcttg ccggtgaccg
     4381 cgcgcatcga caaggccgcg ccgccgcggg cgtcgccgtc gaggcgggtg aggacgaccc
     4441 cggtgagagg cacctggccc gagaagctgg aggcgacgtt gaccgcgtcc tgcccggtca
     4501 aggcgtcgac gacgagcagt atctcggcgg ggctggagac gtcggcgacc gccttcatct
     4561 cgtccatcag ggcctggtcg acgtggagcc ggcccgccgt gtcgaggagg aggacgtcat
     4621 agccctggag gcgcgcggcc tgcagggcgc ggcgggcgat gtcgaccggc tgctggccgg
     4681 cgacgatcgg aagcgtcgcg acctccgcct tctcgccgag cacccggagc tgctcctgcg
     4741 ccgccgggcg ggcgacgtcg agcgaagcca tcatcacctt cttgccggcc ttctcgcgga
     4801 gacggcgggc gatcttggcg gtgctcgtcg tcttgcccga accctggagg ccgaccatca
     4861 tcaccaccgc cggcggagtg acgtcgacgt tgaggtccga ggcttccgcg ccgagcatct
     4921 cgacgagtgc gtcgtggacg atcttgacga cctgctggcc cggagtgatc gagcggagca
     4981 cctgctggcc gaccgcctgc tcggtcgcct tgtcgacgaa ctcgcgcacc accggcagag
     5041 cgacgtcggc ctcgagcagg gcgatccgga cctcgcgcat cgcgctgcga acgtcggcct
     5101 cgttcagcgc accgtggccg cgcagccggt cgaagacggc gccaaggcgg tcgctcagac
     5161 tgtcgaacat gctcttcctt ccaccagcct caagtgccga acgcgaaaaa cgccggcggg
     5221 cgaaacctcg ccggccagcg cgcgccccct gaggcgctgt actatcccaa agggatgtgg
     5281 gggcgcatag ccgcgagggg aagagatggc aagggtgcgg cgctccgctc cccgcgccgg
     5341 ctcacaaaat gaagtcggcg gcgaccgggt tgcccgccgc gacgagcaac agttcgagat
     5401 cgacgctgcc gtcgccgtcg gtgtcgccct gcaccagcca ggtcgtcccg gcgacattct
     5461 catagcgcag ctcgccggca tggttgccga aggcggcact gccgatgaag ctgaacgcct
     5521 ggtctccggc gaggccggca tcggcgtcga tccggccgag gtcgatcctg tcgccggtcg
     5581 agaagtcctc gatcaggtcc gtggcagaag gcgaggagtc cgccgcgccg tcgtagcgga
     5641 agacgtcgtt gccgccgccg ccgcggagcg tgtcgccgcc gaggccgccg gcgatcaggt
     5701 cggcgccggc ggcgccggac agggaatcat cccctgcccc ggcgaaaatc cggaagctgc
     5761 cgtccgtttc gaggcttccg tcgaagtcca tggtctcgtt cgaccggaga agcgcgccgt
     5821 tgatcgtgat cgtcccgccc gctccgagca gggcgtccgc ccaggtgacg tcataatcga
     5881 acccggcccc gccgcgggcg tagcgctcgt cgccggcgga ggagagggtc agattctcca
     5941 ggttgcggag cgccccgtca tgagcgggat cgttgaagtc gatcatgtag tcgccgcgaa
     6001 gga
//
LOCUS       DASWNU010000174         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_474415, whole genome shotgun sequence.
ACCESSION   DASWNU010000174 DASWNU010000000
VERSION     DASWNU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474415"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGB57_03420"
     CDS             <1..577
                     /locus_tag="VGB57_03420"
                     /inference="COORDINATES: protein motif:HMM:NF022779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HEY0130429.1"
                     /translation="VFGSARALLFGGGGFSSETYTDLFNGTDEEGRGFAVYEACLQRR
                     VGKNTHIVFSGQMYAVQRRPGAEGHTAIVPDKKIFNFWKPARDMERVRIEGDEAFEKK
                     FEVYSTHPMEARELLFDTAFRTRLVGLRENGRVFVYVGPEEALVAAKGKDRFEPGSML
                     RSKPGEERVKLMFDDVCASLDLLRALKAKLG"
     gene            complement(666..1367)
                     /locus_tag="VGB57_03425"
     CDS             complement(666..1367)
                     /locus_tag="VGB57_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0130430.1"
                     /translation="MTGLLVSGMCLTLFLLISPASAQLPVDPRVSAAGIPLSPAWRAA
                     VYAHAREKLLHPAWGWRHSERDFLLARQIARDEGLTVDEDVLFAAAFLHDAGAIPPFA
                     KDGIDHAERSAELAEPLLRKAGFPMARYPAVKAAILGHMFDKEAGSGGEAIALHDADA
                     LDFLGATGIARRLSATGAAPDMDSAFKRLEQVYEEIPGRLVTGAAKRMAIPRLVAMRA
                     FFDQLKRETPGRAPL"
     gene            complement(1466..>2140)
                     /locus_tag="VGB57_03430"
     CDS             complement(1466..>2140)
                     /locus_tag="VGB57_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY0130431.1"
                     /translation="VPAEPYEVNPVIERAMRRIFILHADHEQNASTSTVRLAGSSGAN
                     PFACIAAGIACLWGPAHGGANEAALNMLHEIGRPERIPEFIARAKDKDDPFRLMGFGH
                     RVYKNYDPRAKVMQATAAEVLKELGVTDPVFDVARELEQIALSDDYFIEKKLYPNVDF
                     YSGVVLSAIGFPTSMFTALFALARTVGWVAQWNEMISDPEQKIGRPRQLYTGPTQRDY
                     VPVGQR"
BASE COUNT          358 a          672 c          752 g          358 t
ORIGIN      
        1 cgtcttcggc tccgcccgtg ccttgctttt cggcggggga ggcttttcca gcgagaccta
       61 taccgacctg ttcaacggca ccgacgagga agggcggggc ttcgccgtct atgaagcctg
      121 cctccaacgc cgcgtcggca agaataccca tatcgtcttc tcgggccaga tgtacgcggt
      181 ccagcgccgc cccggcgccg aggggcatac cgcgatcgtt ccggacaaga agatcttcaa
      241 cttctggaag cccgccaggg acatggagcg cgtccgtatc gagggggatg aggcctttga
      301 gaagaaattc gaagtctatt cgacccatcc tatggaggcg cgcgagcttc tcttcgacac
      361 cgcgtttcgg acccgtcttg tcgggctcag agagaatggc cgggttttcg tctatgtcgg
      421 gccggaagag gctctggtcg ccgccaaggg caaggaccgc ttcgagcccg gctcgatgct
      481 ccgctccaag cccggcgagg agagggtaaa gctgatgttc gacgacgtct gcgcttcgct
      541 ggacctgctc agggcgctga aggcgaagct ggggtgaggc gtcgccccag cggaggctgg
      601 ggccagaggg tgaagagtgc aggcgccctt caccctctgc gatgccagct gcgccggatg
      661 acggtttaca gcggcgcccg ccccggagtc tcgcgcttaa gctggtcgaa gaaggccctc
      721 atcgccacca gcctcggtat cgccatgcgt ttggcggcgc cggtgacgag ccggcccggt
      781 atctcctcat agacctgctc gaggcgcttg aaggcggagt ccatgtccgg ggcggcgccc
      841 gtcgccgaga gccgccgggc aattccggtg gcgccgagga agtcgagcgc gtcggcgtcg
      901 tggagcgcga tggcttcccc gccggagccg gcttccttgt cgaacatgtg gccgaggatc
      961 gccgctttca cggccggata tctggccatc gggaagccgg ccttgcgaag cagcggctcg
     1021 gcaagctccg ccgagcgctc ggcatggtcg attccgtcct tggcgaaagg cggaatcgcg
     1081 cctgcgtcgt ggaggaaggc ggcggcgaac agcacgtcct cgtcgacggt caggccttcg
     1141 tcgcgggcga tctggcgggc aagcaggaag tcgcgctccg aatggcgcca gccccaggcg
     1201 gggtggagga gtttctcgcg ggcatgggca tagacagcgg cgcgccaggc cggggagagc
     1261 gggatccccg ccgcgctgac cctgggatcg acgggcagct gggccgaggc gggtgagatg
     1321 agcaggaaga gggtcaggca catcccggac accagaagac ccgtcatccc ggacttgatc
     1381 cggggtccat gaacacggct cagtcccaag tcccgccgcc cgtgttcagg gattccggct
     1441 ttcgccggaa tgacgggaga tggagtcaac gctgccccac gggcacgtag tcccgctggg
     1501 tcgggccggt gtagagctgc ctcgggcggc cgatcttctg ctcggggtcg gagatcatct
     1561 cgttccactg ggcgacccat cccacggttc gcgcgagagc gaacaaagcc gtgaacatcg
     1621 aggtcgggaa gccgatcgcc gagaggacga cgcccgaata gaaatcgacg ttcgggtaga
     1681 gcttcttctc gatgaaataa tcgtcgctga gagcgatctg ctcgagctcg cgggcgacgt
     1741 cgaagacggg atcggtgacc ccgagctcct tcaggacctc ggccgcggtc gcctgcatca
     1801 ccttcgcgcg agggtcgtaa ttcttgtaga cgcggtggcc gaagcccatc agccggaagg
     1861 ggtcgtcctt gtccttggcc cgggcgatga actcgggaat ccgctcgggc cggccgatct
     1921 cgtgaagcat gttgagcgcg gcctcgttgg cgcccccatg ggccgggccc cacaggcagg
     1981 cgatgcccgc cgcgatgcat gcgaacggat tggcgcccga cgagcccgcg agccgcaccg
     2041 tcgaggtcga cgcattctgc tcgtggtcgg cgtggaggat gaagatgcgc cgcatcgccc
     2101 gttcgatcac cgggttgacc tcatagggct cggccgggac
//
LOCUS       DASWNU010000175         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_265410, whole genome shotgun sequence.
ACCESSION   DASWNU010000175 DASWNU010000000
VERSION     DASWNU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265410"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1338
                     /gene="mnmE"
                     /locus_tag="VGB57_03435"
     CDS             67..1338
                     /gene="mnmE"
                     /locus_tag="VGB57_03435"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY0130432.1"
                     /translation="MNDTIFALSSGSPPAALAVVRISGPGADPALEALAGKLPPPRRA
                     TLADLRWRNERLDKALVLRFPGPASATGEDVAELHLHGGRAVVAAVLEALAGQDGLRS
                     AEPGEFTRRAFENGRIDLAEAEGLADLLAAETQSQRRAALAMAGGALGPKVESWRQRL
                     LALAAALEAALDFSDEGEVGEAWPPGWTDEVGSLAGEMEVLLAQPSAERLRDGIRVVI
                     AGPPNAGKSSLLNVLVQREAAITSAVPGTTRDLIEAPTAIGGIPLLLIDTAGLRESKD
                     EVEAIGVERGRSSLAAADLVLWLGEPDQAPDRGKTILIHPKCDLPGPSHEADVRVSAT
                     TGEGIRELVAIVSARALALLPAEGEVALNARQREAIRNACRHLRETAGADMLIAAEAL
                     RQARVELDRVTGRAGVEDMLDALFGRFCIGK"
     gene            1372..3222
                     /gene="mnmG"
                     /locus_tag="VGB57_03440"
     CDS             1372..3222
                     /gene="mnmG"
                     /locus_tag="VGB57_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY0130433.1"
                     /translation="MNRDFDLIIVGGGHAGCEAAAVAARMGAKVALVSFDRSQIGAMS
                     CNPAIGGLGKGHLVREVDAFDGLVGRAADEAGVHYRMLNASKGAAVRGPRVQADRRLY
                     KAAIHRMLGAQQGLEIVEGEAAALRLDCRRIAGLDLADGRTLRAKAVVLATGTFLGGK
                     LHFGRTSRAGGRIGERAATALAAQLRGLGLPLARLKTGTPPRLDGRSLDWAALERQES
                     DWDAWTMSPMTSGRIAPQLFCAITRTNLSTHDIIRSNLDRSPLFAGDIGGVGPRYCPS
                     IEDKVHRFADRDGHQIFLEPEGLDDHAIYPNGISTSLPEDVQLAMVRSMAGLERARIL
                     QPGYAVEYDHVDPRILEPTLGVRDVEGLYLAGQINGTTGYEEAAAQGLVAGANAAAWT
                     AGRAPLLFDRSESYIGVMVDDLTLQGVTEPYRMLTARAEFRLRLRADNAEARLTPHAL
                     AAGCVGRERREHFQARQTRRAAIEADLRGVRTGAELRNAGIKVGDDGHRRPLSEWLRF
                     PELNASGLLRLAPALAEFPSRLLEEAVEDHRYAPYVERQQAEVARLRSDENVRVPPAI
                     DYSAIPGLSLEMAERLNAARPSTLGAARRIRGITPAALAAILVHSRRRAA"
     gene            3222..3848
                     /gene="rsmG"
                     /locus_tag="VGB57_03445"
     CDS             3222..3848
                     /gene="rsmG"
                     /locus_tag="VGB57_03445"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY0130434.1"
                     /translation="MDEAEARARLDVPRETIERLEAFEALLRNENERQNLVSRGSLEA
                     LWERHILDSAQLVRFAPAGARTWLDLGTGAGFPGLMVPLFHSAHAVLVESRRLRAEFL
                     RGAASVLGIATKVDILCAKLEAVPARPFDVISARAFAPLPKLLALAARFSTPGTVWIL
                     PKGRNAKSELEAARSSWQGDFRLEPSLTDAEAQIVVATRVRQKPRGRF"
     gene            3845..4624
                     /locus_tag="VGB57_03450"
     CDS             3845..4624
                     /locus_tag="VGB57_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0130435.1"
                     /translation="MIRIAVANQKGGVGKTTTAINLATALAATGWRVLLIDLDPQGNA
                     STGLGVGQALRDRSSYDVLTGGASLEEAVVPTKVPRLDLVPATVDLSGAEIELVALEE
                     RTHRLDNAIDACASRWDVCLIDCPPSLGLLTINALVAAQSILVPLQTEFFALEGLSQL
                     LQTIERIRARFNPELSILGVALTMFDRRNNLSSQVADDVRACLGKVVFETVIPRNVRL
                     SEAPSHGIPALIYDHRCPGSEAYIALARELISRLPRPAEAA"
BASE COUNT          718 a         1597 c         1632 g          761 t
ORIGIN      
        1 ccgggacttc tcgtcccgag gtcccggctt tcgccgggat gacggggcgg tccaaagacg
       61 caggcaatga acgacaccat cttcgctttg tcctccggca gcccgcccgc cgctttggcg
      121 gtggtccgga tcagcgggcc gggcgctgac ccggcgctcg aggcgctggc cgggaagctg
      181 ccgccgccgc gcagggccac gctcgcggat ctgcgctgga ggaacgagcg cctcgacaag
      241 gccctcgttc tgcgctttcc aggccccgcc agcgccaccg gcgaggatgt ggccgagctt
      301 cacctccacg gcggtcgggc ggtggtggcg gccgtcctgg aggctctggc ggggcaggac
      361 gggcttcggt ccgccgagcc gggcgagttc acccgccgcg ccttcgagaa cggccggatc
      421 gacctcgccg aggccgaggg cctcgccgac ctgctcgccg ccgagaccca gtcgcagcgc
      481 cgcgccgcac tcgccatggc cggtggagcg ctcggcccga aagtggagtc atggcgtcag
      541 cggcttctgg cgctggcggc ggcgctggag gcggcgctcg atttttcgga cgaaggcgag
      601 gttggcgagg cctggcctcc gggctggacc gacgaggtcg ggtcgctcgc cggtgaaatg
      661 gaggtgctgc tcgcgcagcc gtcggcggaa cggctaaggg acggtatccg agtggtcatc
      721 gcgggacctc ccaatgcggg aaaatctagt cttctcaacg tgttggtgca aagggaggcg
      781 gcgataacct cggccgttcc ggggacgacc cgagatctca tcgaagcgcc tacggcgatc
      841 ggcggaattc cgttgctgtt gatcgacacg gccggcctta gggaaagcaa ggacgaggtg
      901 gaggctatcg gagtggagcg aggacgttcg agcctcgcgg cggccgacct cgtcttgtgg
      961 ctgggcgagc cggaccaggc gcccgaccgg gggaagacaa ttctgatcca tccgaaatgc
     1021 gacctccccg gcccgagcca tgaggcggac gtccgggttt cggcgaccac cggcgaaggc
     1081 atccgggagc tggtcgcgat cgtttcggcg cgggcccttg cgcttcttcc cgcggagggc
     1141 gaagtcgccc tcaacgcccg ccagcgggag gcgatccgca acgcttgccg ccatcttcgc
     1201 gaaacggccg gcgccgacat gctgatcgcc gccgaagcgc tccgccaggc ccgggtcgag
     1261 ctggaccgag tcaccggccg cgccggggtg gaggatatgc tcgacgcttt gttcggccgc
     1321 ttctgcatcg gcaagtgaca tgttccacgt ggaacatgta gaggcaccgc tgtgaatcgg
     1381 gacttcgacc tcatcatcgt cggcggcggc catgccgggt gcgaagccgc ggccgttgcg
     1441 gcgcgaatgg gcgccaaggt ggcgctcgtc agcttcgacc gctcgcagat cggcgccatg
     1501 tcgtgcaacc cggccatagg cggcctgggc aaggggcatc ttgttcgcga agtcgatgct
     1561 ttcgacggac tggtcggccg tgcggcggac gaggcaggcg tgcattaccg aatgctcaac
     1621 gccagcaagg gcgcggccgt acgcgggccc cgggtgcagg ccgaccgccg cctctacaag
     1681 gcagccatac accggatgct gggcgcccag cagggactgg agatcgtcga aggggaggcg
     1741 gcggcgctcc gcctcgactg tcgccggatc gccggcctgg acctcgccga cggccggacg
     1801 ctccgtgcga aggccgtcgt cctggcgacc ggcaccttcc tcgggggcaa gctccatttc
     1861 gggcggacca gccgggccgg gggcaggatc ggggaacgtg ccgcgacggc gctggcggcg
     1921 cagcttcgag ggctcggctt gcctctggct cgcctcaaga cggggactcc gccccggctc
     1981 gacggccgca gcctcgactg ggccgcgctc gagcggcagg aatcggactg ggatgcctgg
     2041 acgatgtcgc cgatgacgag cggccgcatc gcaccgcagc tcttctgcgc gatcacccgg
     2101 accaaccttt cgacccatga catcatcagg tccaacctgg accgctcgcc tttgttcgcc
     2161 ggcgacatcg gcggggtcgg accgcgttac tgtccgtcta tcgaggacaa ggtccaccgc
     2221 ttcgccgatc gcgacgggca ccagatcttc ctcgagcccg aggggctgga cgatcatgca
     2281 atctatccca acggaatctc cacctcgctt cccgaggacg tccagctcgc catggtcagg
     2341 tcgatggccg gcctcgagcg ggcgaggatc ctccagcccg gctatgccgt cgaatatgat
     2401 catgtcgacc cgcggatcct cgagccgacg cttggcgtcc gcgatgtcga gggcctgtat
     2461 ctggccgggc agatcaacgg cacgaccggc tatgaggagg cggcggcgca gggcctggtc
     2521 gccggggcca atgccgccgc ctggaccgcc ggccgggcgc ctttgctgtt cgaccggagc
     2581 gagagctata tcggggtcat ggtcgacgat ctcacgctcc agggcgtgac cgagccttac
     2641 cggatgctca ccgcgcgcgc ggaattccga ctgcggctcc gcgccgacaa tgccgaggcg
     2701 cggctcactc cgcacgccct tgcggcagga tgcgtcgggc gtgagcggcg cgagcatttc
     2761 caggcgcgcc agacgcgacg tgccgccatc gaggctgatc tccggggcgt tcggaccggg
     2821 gccgagctcc gcaacgccgg gataaaagtc ggcgacgacg ggcaccggcg gccgctctcc
     2881 gaatggctgc gcttccccga gctgaacgca tcgggcctgt tgaggctggc gccggctctg
     2941 gccgaattcc cgagccgtct cctcgaggaa gcggtagagg atcatcgcta cgcgccctat
     3001 gtcgagcgcc agcaggccga ggttgcccgg ctgcgcagcg acgagaacgt ccgcgttccg
     3061 cctgcaatcg actattccgc gatccccggc ctgtcgctgg aaatggccga acgcctgaat
     3121 gctgcacggc cgtccaccct tggtgcggcg cggcggatcc ggggaatcac gccggcggcg
     3181 ctggcggcga ttttggtcca ttcgaggcgc agagcggcct gatggacgaa gcggaagcca
     3241 gggcgcggct cgatgttcca cgtgaaacaa tagagcgcct ggaagccttc gaggcgcttt
     3301 tgcgcaacga gaacgagcgt cagaatctcg tctcgagagg ctcgctcgag gcgctgtggg
     3361 agcgccacat cctcgattcc gcgcagctcg ttcgcttcgc cccggcgggt gcccgcacat
     3421 ggctggatct gggcaccggg gccggctttc ccgggcttat ggtgccgctt ttccattcgg
     3481 cccatgccgt gctggtggaa tcgcggaggc ttcgggccga gttccttcgc ggcgcggcat
     3541 ccgtgctcgg catcgcaaca aaggtcgaca tcctctgcgc gaagctcgag gcggtccccg
     3601 cccggccgtt cgacgtgatc agcgcccgcg ccttcgcgcc cttgcctaag ctgctggcgc
     3661 tggcggcacg tttttccacg cccgggacag tctggatcct gccgaagggc cgcaacgcca
     3721 agtcggaact ggaagcggcg cgatcttcgt ggcagggtga tttccgtctc gaacccagcc
     3781 tcaccgacgc cgaagctcag atcgtcgtcg caacccgggt tcgccaaaag ccgaggggaa
     3841 gattttgatc cggatcgcgg tcgccaacca gaaaggcggg gtcggcaaga cgacgacggc
     3901 gatcaacctc gccaccgctt tggccgccac cggctggcgg gtgctcctga tcgacctcga
     3961 tcctcagggc aacgcctcaa ccggtctcgg cgtcggccag gcgcttcgcg atcgttcgag
     4021 ctacgacgtc ctcaccggcg gagccagcct tgaggaagcc gtggttccga ccaaggtgcc
     4081 gaggctcgac ctggtccccg ccacggtcga cctgtccggc gccgagatcg agctggtggc
     4141 gctcgaggag cgcacccacc ggctcgacaa tgcgatcgac gcctgcgcca gccgctggga
     4201 cgtgtgcctg atcgactgcc cgccgagcct cggccttctg accatcaacg cgctcgtcgc
     4261 ggcccagtcc atattggtcc cgcttcagac ggaattcttt gccttagaag ggcttagcca
     4321 gcttttgcag acgatcgagc ggatccgggc ccggttcaac ccggagcttt ccatcctcgg
     4381 agtggctctg accatgttcg accgccgcaa caacctctcc tcgcaggtcg ccgacgacgt
     4441 ccgcgcctgc ctcggcaagg tggtgttcga gacggtgatc ccgcgcaacg tgcggctttc
     4501 ggaggcgccg agccacggca taccggcgct gatctacgac catcgctgcc ccggttcgga
     4561 ggcctatatc gcactagccc gcgagctgat ctcgcggctg cctcggcctg cggaggcggc
     4621 atgaccatca agaagccggg atccggcctc ggccgtgggc tttccgcttt gctggacgag
     4681 gcggtgcgcc attcgccttc tgttgggg
//
LOCUS       DASWNU010000176        10379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_32751, whole genome shotgun sequence.
ACCESSION   DASWNU010000176 DASWNU010000000
VERSION     DASWNU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32751"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1025)
                     /gene="serB"
                     /locus_tag="VGB57_03455"
     CDS             complement(147..1025)
                     /gene="serB"
                     /locus_tag="VGB57_03455"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY0130436.1"
                     /translation="MFIATLIAKDRLADGDISAAADALAAAGMAPFGRSWIEEDKACD
                     LLFSAAPGPARAALEGLIPGVDVIVQGEASRRRRLLVADMDSTMIPVECIDELADYAG
                     LKAQVAAVTERAMRGEIGFEAALAERVALLAGLDEAAIDRCREERVRLTPGARALVRT
                     MKREGAFTLLVSGGFTRFADPIGAEIGFDRVVANRLGVRAGKLDGTVAAPVLGAERKR
                     LALLDCVAELGLRPDETLAVGDGANDVPMLKEAGLGIAYHAKPPAAAAADSAILHNNL
                     TALLYAQGWARSEWQA"
     gene            1024..1962
                     /gene="miaA"
                     /locus_tag="VGB57_03460"
     CDS             1024..1962
                     /gene="miaA"
                     /locus_tag="VGB57_03460"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041379503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY0130437.1"
                     /translation="MASSSAGRLALIAGPTASGKSALALSLAERSGGTVINADSAQVY
                     ADLRIISARPTAGDEARAPHRLYGYRDGAQACSAAEWAADARREIAAVNGSGGLPVLV
                     GGTGLYINTLLNGIAPVPEIDPAIRSEVRAAPVLHNYAALRGEDPEAAARLRPTDTTR
                     VARALEVVRSTGRPLAQWQKERSGGLAGEVRLAPLILLPERDWVYARCDERFEKMMSE
                     EGLEEVRSLLNRRLDPTLPVMRAIGVREIAACLRGELRREEALEAGRTATRQYAKRQY
                     TWFSRQPPAHWPRFTERLDCGGLANALADLDARLSR"
     gene            1943..2935
                     /gene="ilvC"
                     /locus_tag="VGB57_03465"
     CDS             1943..2935
                     /gene="ilvC"
                     /locus_tag="VGB57_03465"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY0130438.1"
                     /translation="MHVYRDEDCDLALIRGRRVAVIGYGNQGRAQALNLRDSGSAVAI
                     GLPEGSASRARASGDGFDVMTAAEASEKSDVVVMLAADELHARIYSEEVAPNLPEGAA
                     LGFAHGLSIRFALIEPRPDLDVFLVAPKGPGTALRSEYEQGGGLIALFAVHQDPTGGA
                     EALALSYAAAIGSGRAGILRTSFAEECEADLFNEQAVLWGAIPELIHAGFETLVDAGF
                     SPEIAYFECLTEVKLLADLIYERGIAGMREAISNTAEFGALKGGTRIVTADTRTEMRR
                     ILREVKSGGFVRELIADSEAGYPRLKSSRAQAAAHPIERVGARLRALSERGGNG"
     gene            2928..3278
                     /locus_tag="VGB57_03470"
     CDS             2928..3278
                     /locus_tag="VGB57_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130439.1"
                     /translation="MAEAGAWLSRRRLERAFILAVGIAMVWIAWGLWSDRQFPGRFTR
                     LHLGMDRKAAEAILGAPDWEGGCAGSVDYLPRSDCSVELGYASAFAPLRPVYYIVQLD
                     RSGKVIEAEPVHTR"
     gene            3340..4179
                     /locus_tag="VGB57_03475"
     CDS             3340..4179
                     /locus_tag="VGB57_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0130440.1"
                     /translation="MSVRPRPAWLPPPDGSRDLRVEDPTNQWVVHLAGRMLLPLALRA
                     RISANLVSVAGLAVGAGAALAYLGWRDPALATLGFLLTIAWLVFDGLDGMVARATGTA
                     SPLGRFLDGLCDHGVFLILYLALGASIDTPEAWITGTIAGLVHGVQANLFESERARYH
                     RRVRGDPGSGAPPPSPNAAVRFYDRLAGSLDRVAGRFDRALAEAADRRALAAAYGERA
                     APALKLLNLLNANARIIVLYAACLAGDPMLFWWWTLGPLTLLTAAGLIWHRRIEAGLA
                     AGA"
     gene            complement(4289..5542)
                     /locus_tag="VGB57_03480"
     CDS             complement(4289..5542)
                     /locus_tag="VGB57_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130441.1"
                     /translation="MHRSTRLTLALLAFGTGSTLAAAVPRTPLAPAEAERLAASARAC
                     TGGYYKLAGPDKPTIRRSAADHLPALPAAGDDGSTWSEERGDDVFDLAVAMLENSITF
                     RERPFSDSSHLDCPADPASGVRLMRFLAGDGANSRLAASNVHHWLGEAYRTGTGVPAD
                     PVKGRRHFLIARLSGHDQIGPSAWGERPGDSLASLYARPAERAVFEEMAMAARHSGEA
                     QSLIAEIVVRSDPKRARALLEAAAASGHSGARRRLAEHEVNGTLGKRNPARAVELLAQ
                     GTCKRCDTYEPMLTAARAHNGGGEIPTMPRRIGIGEIGGMKRLLPDPEEIERDSLVGT
                     VEARGLMAPDGRILYVELVGRESRVDTLARGTLTMFRPERLARLTPHRVDGKPVFVWV
                     DLPRITWGWDHEKQAPRTMPEASPE"
     gene            complement(5691..7514)
                     /gene="mutL"
                     /locus_tag="VGB57_03485"
     CDS             complement(5691..7514)
                     /gene="mutL"
                     /locus_tag="VGB57_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY0130442.1"
                     /translation="MSIRRLPEHLVNRIAAGEVVERPASALKELIENAIDAGATRIAV
                     SLGGGGTVRIEVADDGCGMSPADMALALERHATSKLPGEEIEAVTTLGFRGEALPSIA
                     SVARLSVESRMRGADGWSRIVDNGRLEGEGPAALPPGTRIRVEQLFARVPARRKFLRS
                     ERSEYAACLDVVKRLAMARPDVGFSLEHDGRRTLSVQPGEDRPARVAALTDRALHENS
                     VAVDYDRGPARLGGVAGLPTFNRGVADHQYLFVNGRPVKDRLLIGAVRAAYQDLLARD
                     RHPVIALFIDLPGDEVDVNVHPAKTEVRFRDPAGIRGLIVGGLRHALDGAGFRSVQRA
                     AADVPWQTSPSPQGREGWGEGALPLQERLYDHRPSSYLGSPSPYPSPRWGEGTVRDTR
                     APYDFAPAARGESAFAPPPEAHSFPLGVARGQVAATYIVAEAEDGLILVDQHAAHERL
                     VLERMRRAMADGGVARQALLLPEVVELDEPACDRLEARATELIEMGLELERFGARAVL
                     VRATPAILGNGDVKGLVTDLADELAAFDDALALKEKLDHVAATMACHGSVRAGRILSV
                     AEMNALLREMEVTPHSGQCNHGRPTWVKLAHGDIEKLFGRK"
     gene            7662..8717
                     /locus_tag="VGB57_03490"
     CDS             7662..8717
                     /locus_tag="VGB57_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0130443.1"
                     /translation="MSLLRFFKFPSHDMAIDLGTANTVVYVRGRGVVLNEPSVVAIET
                     INGVKRVKAVGDDAKMMMGKTPDQIEAIRPLRDGVIADIDVAEQMIKHFIQKVHGKRR
                     FPRWPEIVICVPSGSTSVERRAIRDAASNAGASQVWLIEEPMAAAIGADMPVTEPIGS
                     MVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEAISSYVRRNHNLLIGEATAERIK
                     KQVGIAKAPTDENGNPSDGLSVHIKGRDLVNGVPKEITINQRQIAEALSEPVGTIVEG
                     VRIALENTAPELAADIVDQGIVLTGGGALLQGLDEVLRDETGLPVTVADDPLTCVAIG
                     TGRALEEEIFRGVLLTA"
     gene            8741..9640
                     /gene="mreC"
                     /locus_tag="VGB57_03495"
     CDS             8741..9640
                     /gene="mreC"
                     /locus_tag="VGB57_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY0130444.1"
                     /translation="MAPPSTRRPGFSRRAQYGLFLSYVLAVGGVLFAGLLLVIAIVDP
                     SGFNALKGVALDVTSPVSSGGRSVSSFFGGIGSSVGNYVQAGSQNAALKRRLDESRRR
                     LIEAQATELENKRLKALLGLTRETEDRVAVTRIVGSSFASVRRLATLSAGASAGVQPG
                     QPVRSADGLVGRVIETGRWASRVLLVGDSASNVPVRLVRNGTAAIATGHGDGTIDLKT
                     LELGQNPFRRGDVLVTSGVGGVYPPDIPVAVVVSVAGERTVAKPLADPANVEYAMVLK
                     IYQPLADVPLTEATESALRGAGQ"
     gene            9637..10152
                     /gene="mreD"
                     /locus_tag="VGB57_03500"
     CDS             9637..10152
                     /gene="mreD"
                     /locus_tag="VGB57_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY0130445.1"
                     /translation="MSRIAGSSRDVALLGFRRQWVPILSTLAAALLALFPYVASRPLV
                     PDFAYLVLLAWRLLRPEMWQAHMALPLGLFNDLVVGLPIGQSMALWTLTFLILDVVDS
                     RVGWRDYWLDWLFAAAAILFYTAGGWYVARQMGGSTPFAVLLPQLALSVFAYPLVARL
                     VVALDRWRLQR"
     gene            10149..>10379
                     /locus_tag="VGB57_03505"
     CDS             10149..>10379
                     /locus_tag="VGB57_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0130446.1"
                     /translation="MMKRRNRPVITESSQLFTFTRRALVLGGFQGLLGAALAGRMAYI
                     SIAENERYKLLSESNRVQLRLIPPRRGWIVDRA"
BASE COUNT         1510 a         3635 c         3602 g         1632 t
ORIGIN      
        1 gaggtcgagc ttcgccatcc cgaggccggg gcgatcgcgg cgacggtccg ttcgctggcg
       61 ccggacggag tcgagctccg cttcccgctc ggcgaggcct ccgtcgcctt cgccctcgcc
      121 gccatcgccg ccgacatgac ccgcccctag gcctgccatt cgctccgcgc ccagccctgg
      181 gcgtagagca aggcggtgag gttgttgtgg aggattgccg agtccgccgc ggcggcggcg
      241 ggcggcttgg cgtgataggc gattccgagg ccggcttcct tcagcatcgg aacgtcgttg
      301 gcgccgtcgc cgacggccag ggtctcgtcg ggacggagac cgagctcggc gacgcagtcg
      361 agcagtgcaa gcctcttgcg ctcggctccg agcaccggcg cggcgaccgt cccgtcgagc
      421 tttcccgccc tcacgcccag ccggttggcg accacccggt cgaagccgat ctcggcgccg
      481 atcggatcgg cgaagcgggt gaacccgccg gagacgagca gggtgaaggc cccttcccgc
      541 ttcatcgtcc ggacgagggc ccgggcaccg ggcgtaaggc gcaccctttc ctcgcggcac
      601 cggtcgatcg ccgcttcgtc gagcccggcg agtagcgcga cccgctcggc gagcgccgcc
      661 tcgaagccga tctcgcccct catcgcgcgc tcggtgaccg ccgccacctg cgccttcagc
      721 ccggcataat cggcgagctc gtcgatgcat tcgaccggga tcatcgtcga atccatgtcg
      781 gcgacgagca gccggcggcg gcgcgacgcc tcgccctgga cgatcacgtc gacgcccggg
      841 atcagcccct cgagcgcggc ccgggcgggc ccgggagcgg ctgagaagag caggtcgcag
      901 gccttgtctt cctcgatcca gcttcggccg aagggcgcca tgccggccgc ggcgagggcg
      961 tcggcggccg ccgaaatatc cccgtcggcg aggcgatcct ttgctatcag agtggcgatg
     1021 aacatggctt cttcttcggc tgggcggctg gcgcttatcg cgggaccgac cgcaagcggc
     1081 aagtcggcgc tcgcgctaag cctggcggag aggtccggcg gaaccgtgat caatgccgac
     1141 agcgcgcagg tttatgcgga tttacgcata atctcggccc ggcccacggc cggggacgaa
     1201 gcccgggcac cgcaccgact ctacggctac agggatggcg cccaggcttg ctcggcggcc
     1261 gaatgggcgg cggacgcgcg ccgggagata gcggccgtga acgggtccgg agggctgccg
     1321 gtgctcgtcg gcggcacggg cctctacatc aacaccctgc tcaacggcat tgccccggtg
     1381 ccggagatcg acccggcgat acgctccgag gtccgggcgg ccccggttct gcataattat
     1441 gcagcgctcc gcggcgagga cccggaagcg gcggcccgcc ttcgacccac cgacacgacc
     1501 cgtgtggcgc gcgcgctgga agtggtccgc tcgaccggcc ggccgctcgc gcaatggcag
     1561 aaggagcgca gcggcggcct cgccggcgag gtgaggctgg cccccctcat tctcctcccc
     1621 gaacgggact gggtttatgc tcgctgcgac gagcggttcg agaaaatgat gtcggaagag
     1681 ggccttgaag aggttcgttc gcttctcaat cgaaggctgg acccgacgct gccggtgatg
     1741 cgcgcgatcg gggtgcggga aatcgccgcc tgtctgcgcg gcgagctgag gcgggaggaa
     1801 gcgctcgagg cggggcgcac cgccacccgc caatatgcca agcgccaata tacctggttc
     1861 tcccgccagc cgcccgccca ctggcctcgc ttcaccgagc ggctcgattg cggagggctt
     1921 gcaaatgccc tagccgatct ggatgcacgt ctatcgcgat gaggattgcg acctcgcgct
     1981 gatccgcggc cgtcgcgtcg ccgtgatcgg ctacggcaac cagggacgcg ctcaagccct
     2041 caacctgcgg gacagcggaa gcgccgtcgc catcgggctt ccggaaggat cggccagccg
     2101 cgcccgcgcc tccggcgacg ggtttgacgt catgacggcg gcagaagcct ctgaaaagtc
     2161 ggacgtcgtc gtgatgctgg cggccgacga gctccacgca cgcatttatt ccgaagaagt
     2221 ggctcccaac ctcccggagg gcgccgccct cggcttcgcg cacgggctca gcatccgctt
     2281 cgcactgatc gagccgaggc cggacctcga cgtcttcctg gtcgcgccca aggggcccgg
     2341 caccgctctg cggagcgaat atgagcaggg cggcggcctc atcgccctgt tcgccgtcca
     2401 ccaggatccg accggcgggg ccgaggcgct cgctctctcc tacgccgccg cgatcggaag
     2461 cggccgggcc ggcatcctcc ggaccagctt cgccgaggaa tgcgaggcgg acctgttcaa
     2521 cgagcaggcg gtcctgtggg gcgcgattcc ggagctgatc catgccggct tcgaaaccct
     2581 ggtcgatgcc ggcttctcgc cagaaatcgc ttatttcgaa tgccttaccg aggtgaagtt
     2641 gctggccgac ctcatctacg agcgcggcat agcggggatg cgcgaggcga tctccaacac
     2701 cgccgagttc ggcgccctga aaggcggcac ccggatcgtc accgccgaca cccgcaccga
     2761 gatgcgccgg atcctgcgcg aagtgaaaag cggcggcttc gtccgcgagc ttatcgccga
     2821 ctccgaagcc ggctatccgc ggctgaaatc gagccgggcg caggccgccg cccacccgat
     2881 cgaacgggtc ggcgcccgat tgagggcgct ttcggagcgc ggcggcaatg gctgaggcgg
     2941 gcgcctggct gtcgcggcgg cggctcgagc gggcgttcat cctcgcggtg ggcattgcaa
     3001 tggtctggat cgcctggggc ttgtggagcg accggcagtt tccaggccgc ttcacccggc
     3061 tccatctcgg aatggaccgc aaggcggccg aagcgatatt gggcgcgccc gactgggaag
     3121 gcggctgcgc cggctccgtc gactatctgc cgaggtccga ttgctcggtc gagctcggct
     3181 atgcctcggc cttcgccccg ctgcgcccgg tttattacat cgtccagctc gaccggagcg
     3241 gcaaggtcat cgaggccgag ccggtgcaca cgcggtaaat cggggacgct gctccagttg
     3301 attgaccgcg atccgcaccg gggtttaacg gcggcggcga tgagcgtgcg tccccgcccc
     3361 gcctggctgc cccctcccga cggctcccgc gaccttcgag tcgaggaccc gaccaaccag
     3421 tgggtcgtcc atctcgccgg gcgaatgctc ctgccgctgg cgctccgggc gcggatctcg
     3481 gccaacctgg tctcggtggc ggggctcgcc gtcggcgccg gagcggcgct cgcctatctc
     3541 ggctggcgcg atccggcgct cgccaccctc ggcttcctac tcaccatcgc ctggctggtc
     3601 ttcgacgggc tcgacggaat ggtcgcgagg gccaccggca ccgcgagccc gctggggcgc
     3661 ttcctcgacg gactgtgcga ccatggcgtc ttcctgatcc tctacctcgc gctcggagcg
     3721 tcgatcgaca cgcccgaggc ctggatcacc ggcacgatcg ccggcctcgt ccatggcgtc
     3781 caggccaatc tcttcgagag cgagcgcgcc cgctaccatc gccgggtccg aggcgatccc
     3841 ggaagcgggg cccctccccc ctcgcccaac gcggcggtgc gcttctacga ccggctggcg
     3901 ggctccctcg accgggtcgc cggccgcttc gaccgcgccc tcgccgaggc cgcggaccgc
     3961 cgggccctgg ccgccgccta tggcgagcgc gcggcgccgg cgctgaagct cctcaacctg
     4021 ctcaacgcca atgcccggat catcgtcctc tatgccgcct gcctcgccgg ggacccgatg
     4081 ctgttctggt ggtggacgct cggcccgctg accctgctga ccgcggcggg cctgatctgg
     4141 cacaggcgga tcgaggccgg tctggccgca ggcgcataga gtccggccgg cctcctccct
     4201 ccctgcaatt ccagccactt agaattcggt gtctgacacc gattttcctg gcggcgggct
     4261 tgggtcccgc ccgccgggga gaagggcttc actccggaga ggcttcgggc atggttcgcg
     4321 gcgcctgctt ttcatggtcc cagccccagg tgatccgcgg caggtcgacc cagacgaaca
     4381 ccggcttgcc gtcgacgcgg tgcggagtca gccgcgccag ccgctccggg cggaacatgg
     4441 tcagcgtccc tctcgccagc gtatcgacgc gggactcgcg tccgaccagc tcgacgtaaa
     4501 ggattcgacc gtccggagcc atcaggcccc gcgcctcgac cgtgccgacc agcgagtccc
     4561 tttcgatctc ctccggatcg ggaaggagcc tcttcatgcc gccaatctcg ccgatcccga
     4621 tccgcctggg catggtgggg atctccccgc cgccgttgtg ggcgcgcgcg gccgtgagca
     4681 tgggctcgta agtgtcgcac ctcttgcagg tcccctgcgc aagcagttcg acggcgcgcg
     4741 cggggttgcg ctttccaagc gtcccgttga cctcatgttc ggccagcctt ctccttgcgc
     4801 cggaatggcc ggaggcggct gcggcttcga gcagggctct ggcgcgcttc ggatcgctcc
     4861 tgacgacgat ctcggcgatg agggactgcg cttcgccgga atgtcgggca gccatcgcca
     4921 tctcctcgaa gaccgcgcgc tccgccggcc gggcgtaaag gctggcgagc gaatcgcccg
     4981 gccgctcgcc ccaggcggac ggaccgatct gatcgtggcc gctcagcctc gcgatgagga
     5041 agtggcgtcg ccccttgacg ggatcggccg ggactcccgt tcccgtccgg tacgcttccc
     5101 ctaaccaatg atggacgttc gaggcggcga gccgggagtt ggctccgtcg cccgcgagga
     5161 aacgcatgag ccggaccccc gacgcgggat ccgccgggca gtcgaggtgg ctggaatcgg
     5221 agaaaggccg ctccctgaag gtgatcgagt tttcgagcat cgcgacggcg agatcgaaca
     5281 catcgtcgcc gcgctcctcc gaccaggtgg acccgtcgtc cccggccgcg ggaagggcgg
     5341 ggagatgatc ggccgccgag cgccggatcg tcggcttgtc gggaccggcc agcttgtaat
     5401 agcccccggt gcaggcgcgg gccgaagccg ccagccgctc ggcctcggcg ggcgcgagcg
     5461 gggtccgcgg aaccgccgcc gccagtgtgg agcccgtgcc gaaggcgagc agggccaggg
     5521 tcagcctagt cgaacgatgc atatccaaac ccccggagaa cgctgcggaa attattactc
     5581 cgggaccttt cgagcaatag cggctttggc cgaatgatcc ggaatggagg tgcgatgagc
     5641 gtgggcgcgc gcggcggacc tcggctccgc ccccgccgac ccgctcccgc ctatttccgc
     5701 ccgaacaatt tctcgatatc cccgtgcgcc agcttcaccc aggtggggcg gccgtggttg
     5761 cattggccgc tgtggggggt gacctccatc tcgcgcagga gggcgttcat ctcggcgacg
     5821 gagaggatgc ggccggctcg gaccgagccg tggcaggcca tggtggcggc gacgtggtcg
     5881 agcttctcct tgagggcgag ggcgtcgtcg aaggcggcga gctcgtcggc gaggtcggtg
     5941 accaggccct tgacgtcgcc attgccgagg atggcggggg tggctcggac cagcaccgcc
     6001 cgggcgccga agcgctcgag ctcgaggccc atctcgatca gctccgtcgc gcgcgcctcg
     6061 agccggtcgc aggccggctc gtccagctcg acgacctcgg ggaggagcag ggcctggcgc
     6121 gcgactccgc catcggccat cgcccggcgc atccgctcca gcaccagcct ttcgtgggcg
     6181 gcatgctggt cgacgaggat caggccgtcc tcggcctcgg cgacgatata ggtggctgcg
     6241 acctggcccc gggcgacccc gagcgggaag ctgtgggcct ccggaggcgg agcgaaggcc
     6301 gattcacctc gggcggcggg ggcgaaatcg tagggcgcgc gggtgtcgcg aaccgtcccc
     6361 tcgccccagc ggggagaggg atagggtgag ggggaaccca aatacgagga tgggcgatga
     6421 tcgtagagcc tctcctggag aggcagagcc ccctcgcccc agccctctct cccctgggga
     6481 gagggggaag tttgccacgg cacgtcggcc gccgcgcgct gcacgctccg gaaccccgcc
     6541 ccgtcgagcg cgtgcctgag gccgccgacg atcagccccc ggatgcccgc cgggtcgcgg
     6601 aatcggacct cggtcttggc ggggtggacg ttgacgtcga cctcatcgcc gggaaggtcg
     6661 atgaacaagg cgatgaccgg atggcggtcg cgggcgagaa ggtcctgata ggcggcccgg
     6721 acggcgccga tcaggagccg gtccttcacc ggacggccgt tgacgaacag atattggtgg
     6781 tcggcgaccc cgcggttgaa ggtcggaagc ccggcgaccc cgcccaatcg ggcaggccct
     6841 cggtcgtagt cgacggcgac gctgttctcg tggagggctc gatcggtcaa ggcggcgact
     6901 cgggccggcc ggtcctcgcc gggctggacc gagagggtcc ggcggccgtc atgctcgagg
     6961 ctgaagccga cgtcgggccg ggccatggcg agccttttca cgacgtcgag gcaggcggca
     7021 tattccgacc gctccgagcg caggaacttg cggcgggcgg ggacgcgggc gaacaattgc
     7081 tcgaccctta tcctcgtgcc gggcgggagc gccgccggcc cctctccttc gagccggccg
     7141 ttgtcgacga tgcgcgacca gccgtcggcc ccgcgcatcc ggctctcgac ggaaaggcgg
     7201 gcgacgctgg cgatggaggg aagcgcctcg ccgcggaagc ccagagtggt caccgcctcg
     7261 atctcctcgc cgggcagctt cgaggtggcg tggcgctcga gcgcgagggc catgtcggcg
     7321 gggctcattc cgcagccgtc gtcggcgacc tcgatcctca ccgtgccgcc gccgcccagc
     7381 gagacggcga tccgcgtcgc tccggcgtcg atcgcgttct cgatgagctc cttcaacgcg
     7441 ctggccggtc tttccaccac ttcacccgcc gcgatgcggt tgaccagatg ctcgggcagg
     7501 cggcgtattg acatcgatca ggtccctagc ccaagcgacg ctatcccgcg aggcttgtta
     7561 actcatttgt ggaacaagcc gacgggccat cctataggga aaggcgagcg cgccgggcac
     7621 catgccgcgg cggcgttttt taagaagcgg gacccagctc aatgtcactc ttgcgtttct
     7681 tcaagttccc ttcccacgac atggcgatcg acctcgggac ggcgaacacc gtcgtctacg
     7741 tgcgcggccg cggcgtggtc ctgaacgagc cttcggtggt cgcgatcgag acgatcaacg
     7801 gggtgaagcg cgtgaaggcg gtcggcgacg acgccaagat gatgatgggc aagacgcctg
     7861 atcagatcga agccattcgc cccctcaggg acggagtcat cgccgacatc gacgtcgccg
     7921 agcagatgat caagcatttc atccagaagg tccacggcaa gcgccgcttc ccgcgctggc
     7981 cggagatcgt gatctgcgtg ccctccggct cgacctcggt cgagcggcgc gcgatccgcg
     8041 acgccgcctc caacgccggc gcttcgcagg tgtggctgat cgaggagccg atggcggcgg
     8101 cgatcggagc cgacatgccg gtcaccgagc cgatcggatc gatggtcgtc gacatcggcg
     8161 gcggcaccac cgaggtggcg gtcctctcgc tccgcggcct cgcctatacg acgtcggtgc
     8221 gcgtcggcgg cgacaagatg gacgaggcga tcagctccta cgtccgccgc aaccataacc
     8281 tcctgatcgg cgaggcgacg gcggagcgga tcaagaagca ggtcgggatc gccaaggcgc
     8341 cgaccgacga aaacggcaac ccgtccgacg gtctctcggt ccacatcaag ggccgcgatc
     8401 tcgtcaacgg cgtgcccaag gagatcacga tcaaccagcg acagatcgcc gaggctctgt
     8461 cggagccggt cgggacgatc gtcgagggcg tccgaatcgc actcgagaac accgcgccgg
     8521 agctcgccgc cgacatcgtc gaccagggca tcgtcctgac cggcggcggc gccctcctgc
     8581 aggggctgga cgaggttctt cgcgacgaga cggggctccc cgtcacggtc gccgacgatc
     8641 cgcttacctg cgtggccatc ggcaccggcc gggcgctcga ggaggaaatc ttccgcggcg
     8701 ttctgctgac cgcctgaggc cgggcctggg aggctcacag gtggcaccgc catcgacccg
     8761 ccgccccgga ttctcgcgcc gggcgcaata cggtctgttc ctgtcctatg tgctcgccgt
     8821 gggcggagtg ctcttcgccg ggctgctgct cgtcatcgcc atcgtcgatc cgagcgggtt
     8881 caacgccttg aagggcgtgg cgctcgacgt caccagcccc gtgtcctcgg gcggacgctc
     8941 ggtttccagc ttcttcggcg ggatcggctc ttcggtcggc aattacgtgc aggcgggaag
     9001 ccagaacgcg gcgctgaagc ggcggctcga cgaatcgcgg cggcggctga tcgaggccca
     9061 ggccaccgag ctcgagaaca agcgcctgaa ggccctgctc ggcctgaccc gcgagaccga
     9121 ggacagggtc gcggtcaccc ggatcgtcgg ctcctccttc gcttccgtgc gccgcctcgc
     9181 caccctctcg gcaggcgctt ccgccggagt ccagccgggc cagccggtcc gttcggccga
     9241 cggcctcgtc ggccgggtga tcgagacggg acgctgggcg agccgggtcc tgctcgtcgg
     9301 cgacagcgcc agcaacgtcc cggtccggct ggtccgcaac ggaaccgcgg ccatcgctac
     9361 cggccatggc gacggaacga tcgacctcaa gaccctggaa ttggggcaga atcccttccg
     9421 ccgcggcgac gtgctggtga cttcgggggt gggcggggtc tatccccccg acattccggt
     9481 cgcggtcgtg gtctcggtcg ccggcgagcg cacggtggcg aagccgctcg ccgatccggc
     9541 caatgtcgaa tatgcgatgg tcctcaaaat ctaccagccc ctcgccgacg tcccgctcac
     9601 cgaagccacc gagagcgccc tgaggggggc ggggcagtga gccggatcgc cggatcgagc
     9661 cgcgacgtcg cgctgctcgg cttccgacgc cagtgggtgc cgatcctgag cactctggcc
     9721 gccgccctgc tcgccctttt cccttatgtc gcgagccggc cgctcgtccc cgatttcgcc
     9781 tatctggtgc ttctcgcctg gcggctgctt agaccggaga tgtggcaggc gcacatggcc
     9841 ctgccgctcg gcctgttcaa cgatctcgtc gtgggcctcc cgatcgggca gtcgatggcc
     9901 ttgtggaccc tcaccttcct catcctcgac gtggtcgaca gccgggtcgg gtggcgcgat
     9961 tactggctgg actggctgtt cgccgccgca gcgatcctct tctacacggc cggcggctgg
    10021 tacgtggccc ggcagatggg cgggtcgacg cccttcgccg tgctactgcc tcagctcgct
    10081 ctgtcggtct tcgcttatcc cctggtggcg cggctggtcg tcgccctcga tcgctggcgg
    10141 ctgcagcgat gatgaagagg cgcaaccgtc ccgtcatcac cgagagcagc cagctcttca
    10201 ccttcacccg gcgcgccctg gtgctgggcg gcttccaggg cctgctgggc gcggctttgg
    10261 ccggccgcat ggcgtatatc tcgatcgccg agaacgagcg ctacaaattg ctctccgaga
    10321 gcaaccgggt ccagctccgg ctgattccac cgcggcgggg ctggatcgtc gaccgggcg
//
LOCUS       DASWNU010000177         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_188839, whole genome shotgun sequence.
ACCESSION   DASWNU010000177 DASWNU010000000
VERSION     DASWNU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188839"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VGB57_03510"
     CDS             complement(<1..1012)
                     /locus_tag="VGB57_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY0130447.1"
                     /translation="MDVKTGEMGGCPVDRPTTVRSLLGRTNRDWWPESLPLEILNQGG
                     LSPDPMGADFDYAEAFNGLDYTALKADLTALMTDSQPWWPADYGNYGGLFIRMAWHAA
                     GTYRTADGRGGANSGQQRFAPLNSWPDNGNLDKARRLLWPIKRKYGKNISWADLFILA
                     GNVAIESMGGPVMGFGGGRRDVFEPERDIYWGAEEKWVNEGVETRIDPERQKELEHPL
                     AAIQMGLIYVNPEGPGGKPDPLGSARDIKTTFERMAMNHEETVALTAGGHTFGKAHGA
                     GDPSLVDSATEAADIALQGLGWASAHESGFGEHTITSGIEGAWVNTPTEWSENYFRLL
                     LDY"
     gene            1226..1459
                     /locus_tag="VGB57_03515"
     CDS             1226..1459
                     /locus_tag="VGB57_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130448.1"
                     /translation="MRRGYRWALVATGTAVVAASAALAAETIVYRYDARGRLVKIERS
                     GTVNNNVATSYGFDKADNRVVKTTTGSPNPPPP"
     gene            1563..>1940
                     /locus_tag="VGB57_03520"
     CDS             1563..>1940
                     /locus_tag="VGB57_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130449.1"
                     /translation="MKTTSLRAKALACALLTGAALCGLAAPASAQSPTYRNLDSNGVD
                     LVKGDFLTSFPEGSIGSGEAELVLLRMLGATGTNGTQGSSQWDHILFNIVSSGTYVDF
                     GSRNDKFPGAETRGASLAGSGNFY"
BASE COUNT          322 a          632 c          641 g          345 t
ORIGIN      
        1 cataatcgag gagcaggcgg aaatagtttt ccgaccactc ggtcggcgtg ttcacccacg
       61 caccctcgat accgctggtg atcgtgtgct cgccgaagcc gctttcgtgg gcgctcgccc
      121 agcccaggcc ctgaagcgcg atgtcggccg cctcggtcgc ggaatcgacc aggctggggt
      181 cgcccgcgcc atgggccttg ccgaaggtat ggccgccggc ggtgagagcc accgtctcct
      241 cgtggttcat cgccatgcgc tcgaaagtgg tcttgatgtc gcgcgccgag cccagcgggt
      301 cgggcttgcc gcccgggcct tccggattga cgtagatgag gcccatctgg atcgccgcga
      361 gcgggtgctc gagctccttc tggcgttcgg gatcgatgcg cgtctccacg ccctcgttga
      421 cccatttctc ctcggcgccc cagtagatgt cgcgctccgg ctcgaacacg tcccggcgcc
      481 cgccgccgaa acccatgacc ggtccgccca tcgattcgat ggcgacgttg ccggcgagga
      541 tgaagaggtc ggcccagctg atgttcttgc cgtacttccg cttgatcggc cacagcaggc
      601 gccgcgcctt gtcgagattg ccgttgtcgg gccaggagtt caacggggcg aagcgctgct
      661 ggccgctgtt ggcgccgcca cgcccgtcgg cggttcgata ggttcccgcg gcgtgccagg
      721 ccattcggat gaagaggccg ccgtaattgc cgtaatcggc cggccaccag ggctggctgt
      781 cggtcatcag ggcggtgagg tcggccttga gcgccgtgta atcgagcccg ttgaacgctt
      841 cggcataatc gaagtcggcg cccatcggat cgggcgaaag gccgccctgg ttgaggatct
      901 caagcggaag cgactccggc caccagtcgc ggttggttcg accgagcagc gagcggacgg
      961 tcgtcggccg atccaccggg caaccgccca tttcacccgt tttcacgtcc atgtctctgg
     1021 cctctccttg cgccggtttt cgggcacgct aggcgctgcg ccttatcgga ggaagcgagg
     1081 aaaaacgctt agagcgagcg agaaaatcga tcaggagatc cggccgtcct ctcaacgacc
     1141 attcctccag ttcggatgca acagaatttg tcacggagcc ataaccatgt tccattcgtg
     1201 ttccgattcg gggttggagg ggcgcatgag gcgaggatac aggtgggcgc tggtggccac
     1261 gggcacggcc gtcgtggcgg cgtcggcggc gctggcggcc gaaacgatcg tttaccgcta
     1321 tgacgcccgc ggccggctgg tgaagatcga gcggagcggc accgtgaaca acaacgtcgc
     1381 taccagctac ggtttcgaca aagcggacaa ccgggtcgtg aagacgacca cgggatctcc
     1441 caatccaccg ccaccctgag gcatcgggtc agggcgaggc cctcgatcga tgagcgattg
     1501 aggcgccgcg ggcgccgcgc cggagccggg atcggatccg ggccggattt tggagaagag
     1561 agatgaagac gacgtccttg cgggcgaaag cccttgcttg cgccctgctc accggagccg
     1621 ctctttgcgg cctcgccgcc ccggcctcgg cccagagccc gacctatcgc aacctcgact
     1681 cgaacggggt cgacctggtc aagggcgatt tcctgacgag ctttccggaa ggctcgatcg
     1741 gctcgggcga ggccgagctc gtcctgctcc ggatgctggg cgccaccgga accaacggca
     1801 cccagggatc gagccagtgg gaccatatcc tcttcaacat cgtctcttcg ggcacctatg
     1861 tcgacttcgg ctcgaggaac gacaaattcc cgggcgccga gactcgcggc gcttcgctgg
     1921 ccggaagcgg caacttctac
//
LOCUS       DASWNU010000178         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_188844, whole genome shotgun sequence.
ACCESSION   DASWNU010000178 DASWNU010000000
VERSION     DASWNU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188844"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VGB57_03525"
     CDS             complement(<1..873)
                     /locus_tag="VGB57_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130450.1"
                     /translation="MSARYLCCDEHRRSALADPAAPPGLSGIDYVEVAAGATTADPTL
                     IDIVLVKPLPLAAAPLTGTNIAITGGVRFAAPRVDANVVAMLGATASPPGGSVDRYRV
                     TVPGGQQTDFSTYRIALVSGPTDPTPPAFIDPRLAAVDFSFKIGCPSDFDCAPCDEGG
                     DPPPADPLFDYRARDYPGFRRQLRDRLTALVPGFREDDPVDFTTTLVEAAAYLADQKS
                     YRLDWVGTEAFLQTARSRSSIARHARLLDYDIGEGASARLYAQFDFQPVAPVTDGMPL
                     ALGTPLLARIEGEKA"
     gene            complement(870..1244)
                     /locus_tag="VGB57_03530"
     CDS             complement(870..1244)
                     /locus_tag="VGB57_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY0130451.1"
                     /translation="MSRLAFPFVPAANGRSAVVDYGSDAHVRQMLELLILTLVGERVM
                     RPDLGSPVRQMLFGAGEGPVAIALAATLQATITQHLGHVLTLHELDVAYDEGAAALDI
                     VVGYEVLASLSQDTLQLRAGVA"
     gene            complement(1241..1564)
                     /locus_tag="VGB57_03535"
     CDS             complement(1241..1564)
                     /locus_tag="VGB57_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130452.1"
                     /translation="MLHTGASATCPHGGTLNIIAASPRVTLGGMPAAVLTDQGLVAGC
                     AFSVPGPKPQPCVTTRWIAAATRVLANGQPVLINPLTAICQSVEQIPAGPPIITASQT
                     RVVAT"
     gene            complement(1585..2097)
                     /locus_tag="VGB57_03540"
     CDS             complement(1585..2097)
                     /locus_tag="VGB57_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY0130453.1"
                     /translation="MSSGPLYFGKYRATVLNNTDPMFQGRVQVQLGDRYGLFPSTWAL
                     PAFPMAGKGCAVVALPQIGSMVWVEFEAGDTDYPIWTGAFWPNSGSYPPLALAAATPA
                     TPNILLQTSTGTSVTLSDNPAQNVMIKTTTGATVMIGAAGITISNGQGASIAMTGPSV
                     IINNGALTIT"
     gene            complement(2094..3212)
                     /locus_tag="VGB57_03545"
     CDS             complement(2094..3212)
                     /locus_tag="VGB57_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130454.1"
                     /translation="MATLKGVTLTLLMGPVAVAPAPVAVVEALESAQVTSAVGQRSGF
                     QLVFNYSKTSPIATTLLPAGYFDPLIRVILVATLNGTPTVLADGPIKLQNVAASAKPG
                     ENKLTITGEDVSGYMDLIDFTGFPYPAMPPFARVAVMMAKYAMFGVIPVTIPPLFQAI
                     TIPIDKYSHQAGTDFAYATKLAQEASHEFYVTPGPAPGTNTAYWGPQIRVGLPQPALT
                     VDMDHAGNIDALSFTADGNASELRIVHVKQAGFSIPIPVPNISLLKPPLAARMLTPTK
                     YKLIPTERKKLPEVMMAALTGPAAADPVSATGSLDVTRYGRPMGARTLVGVRGAGLAH
                     DGLWYVRSVTSTLGRGSWKQSFQLSREGLVSNTPVVPL"
     gene            complement(3199..>3410)
                     /locus_tag="VGB57_03550"
     CDS             complement(3199..>3410)
                     /locus_tag="VGB57_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130455.1"
                     /translation="LDRYAAAGRHRVTGGERIDNIAAEAFGDSEQYWKICDANGDADP
                     ALAASPDGRLLVIPLPLEISDHGDS"
BASE COUNT          534 a         1099 c         1249 g          528 t
ORIGIN      
        1 cgctttctcg ccctcgatgc gggcgaggag gggggtgccc aaagcgagcg gcatgccgtc
       61 tgtgacaggc gccaccggct ggaagtcgaa ctgggcgtag agccgggcgc tggcgccctc
      121 gccgatgtcg tagtcgagca ggcgggcgtg gcgggcgatc gagctgcggc tccgcgccgt
      181 ctggaggaac gcctcggtgc cgacccaatc gagacggtaa ctcttctggt cggcaagata
      241 ggcggccgcc tcgacgaggg tggtggtgaa gtcgaccgga tcgtcctcgc ggaagcccgg
      301 caccaaagcg gtcaggcgat cccggagctg acggcggaag ccgggatagt cgcgtgcccg
      361 atagtcgaac agcgggtcgg cggggggcgg gtcgccgcct tcgtcgcagg gcgcgcagtc
      421 gaagtcgctc ggacagccga tcttgaacga aaagtcgacg gccgcgaggc gggggtcgat
      481 gaaggcgggc ggcgtcggat cggtcgggcc ggagacgagc gcgatccggt aggtcgaaaa
      541 atcggtctgc tgcccgcccg ggacggtgac ccggtagcgg tcgaccgacc cgcccggcgg
      601 actcgccgtg gcgccgagca tcgcgacgac gttggcgtcg acccgcggag cggcgaagcg
      661 gacgccgccc gtgatcgcga tgttggttcc ggtcagcggc gcggcggcca gcggcagcgg
      721 tttgaccagg acgatgtcga tcagcgtcgg atcggcggtg gtcgcccccg ccgcgacttc
      781 gacataatcg atgccgctca gccccggcgg ggcggcggga tccgcgagcg cggaccgcct
      841 gtgctcgtcg cagcagaggt agcgggcgct cacgcgaccc ccgcgcgcag ttgcagcgtg
      901 tcctggctca aactcgccag cacctcgtag ccgacgacga tgtcgagcgc cgccgccccc
      961 tcgtcatagg cgacgtcgag ctcgtggagg gtcaggacgt ggccgagatg ctgggtgatc
     1021 gtcgcctgca acgtcgccgc gagggcgatc gccaccggcc cctcgccggc cccgaacagc
     1081 atctgccgca ccggactgcc gagatcgggc cgcatcaccc gctcgccgac cagggtcagg
     1141 atcagcagct cgagcatctg ccggacatgc gcgtcgctgc cataatcgac gacggcgctg
     1201 cgcccgttcg cggccgggac gaaggggaag gcgaggcggc tcacgtcgca accacccggg
     1261 tctgggaagc ggtgatgatc ggcggaccgg cgggaatctg ctcgaccgac tggcagatcg
     1321 cggtcagcgg gttgatcagc accggctggc cgttcgcgag cacccgcgtt gcggcggcga
     1381 tccagcgggt ggtgacgcag ggctgcggct ttggccccgg tacggagaag gcgcagccgg
     1441 cgaccagccc ctggtcggtc agcaccgccg ccggcatccc gcccagcgtc accctcgggc
     1501 tggcggcgat gatgttgagc gtcccgccat gggggcaggt cgcgctcgct cctgtatgca
     1561 gcaccggtcc cggcattttc ccccctaggt gatggtcagc gccccgttgt tgatgatgac
     1621 ggaaggcccg gtcatcgcga tcgacgcgcc ctggccgttg ctgatcgtga tccccgcggc
     1681 gccgatcatg accgtcgccc cggtcgtggt cttgatcatc acgttctggg cggggttgtc
     1741 ggacaaggtg acgctcgtgc ccgtcgacgt ctgcagcagg atgttgggtg tcgccggagt
     1801 ggccgcggcc agcgccagcg gcgggtagct ccccgaattc ggccagaagg ccccggtcca
     1861 gatcggataa tcggtgtcgc cggcctcgaa ctcgacccac accatcgagc cgatctgggg
     1921 caaagcgacg acggcgcagc ccttgccggc catcggaaag gcaggcagcg cccaggtcga
     1981 ggggaacagt ccgtagcggt cgccgagctg gacctggacc cggccctgga acatcgggtc
     2041 ggtattgttg agcaccgtcg cccgatactt gccgaaatag agcgggccag agctcatagc
     2101 ggcaccaccg gcgtgttcga gaccagccct tcgcgcgaga gctggaagct ctgcttccag
     2161 cttccccggc ccagcgtgct ggtcaccgat cgcacgtacc acaggccgtc atgggcgaga
     2221 ccggcgccgc gcacgccgac cagcgtgcgc gcgcccatcg gccggccgta gcgggtgacg
     2281 tcgagacttc ccgtcgcgga gaccgggtcg gcggcggcag ggccggtcag cgccgccatc
     2341 atcacttcgg gcagcttctt gcgctcggtc ggaatcagct tgtacttggt tggggtcagc
     2401 atccgcgccg cgaggggcgg cttcagcagg ctgatgttgg ggaccgggat cggtatcgag
     2461 aagccggcct gcttgacgtg gacgatcctc agttcggagg cattgccgtc ggcggtgaac
     2521 gacagggcgt cgatatttcc ggcatgatcc atgtcgacgg tgagcgccgg ctgcggcagc
     2581 ccgacacgaa tctgcggccc ccaataagcg gtgttggttc ccggggcggg ccccggggtg
     2641 acgtagaatt cgtgactggc ctcctgggcg agcttggtcg cgtaggcgaa gtcggtgccg
     2701 gcctggtggg aatatttgtc gatcggaatg gtaatcgctt ggaagagcgg cgggatcgtg
     2761 accgggatga ctccgaacat cgcatatttc gccatcatca ccgcgacgcg cgcgaatggc
     2821 ggcatcgcgg ggtagggaaa gccggtgaaa tcgatcaggt ccatatagcc cgacacgtcc
     2881 tccccggtga tggtgagctt gttctcgccc ggcttggccg acgcggcgac gttctggagc
     2941 ttgatcggcc cgtcggcgag caccgtggga gtcccgttga gcgtggcgac gaggatgacc
     3001 cggatcaacg ggtcgaaata acctgcggga agcaaagtcg tcgcgatcgg cgaggtcttc
     3061 gaatagttga agacgagctg gaagcccgac cgctgtccga ccgcgctggt gacttgcgcg
     3121 ctctcgagcg cctcgaccac cgcgaccggc gccggcgcca ccgcgaccgg tcccatcagc
     3181 agggtcaggg tcacgccctt aagagtcgcc atgatcggag atctccagcg gcagggggat
     3241 gaccaggagc cgtccgtcgg gcgaggcggc caatgcgggg tcggcgtcgc cgttggcgtc
     3301 gcagatcttc caatattgct cggagtcgcc gaacgcttcg gcggcgatgt tgtcgatccg
     3361 ctcgccgccg gtcacccggt gccggccggc cgcggcgtag cggtcgagcg
//
LOCUS       DASWNU010000179         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_32823, whole genome shotgun sequence.
ACCESSION   DASWNU010000179 DASWNU010000000
VERSION     DASWNU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32823"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="VGB57_03555"
     CDS             <1..1450
                     /locus_tag="VGB57_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130456.1"
                     /translation="PIYLRQRSIDNPTIRAEIARSEHPRKAALLDMYDLHFGPWDTLA
                     ENHPFYGGEKMPAGAGFYPKDLTKADFEAYLVANPGQKEALTSGYTVVKRRGDRLVAV
                     PYGTEYRQWLEPAAKLLERAAARTSNASLAKFLNLRARAFRTNDYYESELAWMEVGGT
                     PIEVVIGPYEVYTDTLYGTKTAFEAFITVQDPRESAALTKYKALLPDMERNLPVEDRY
                     KNFNRPFASPILVADQVHGGGDNVPGVQTIAFNLPNDERVREAKGAKKVILANVLGAK
                     YDRILAPMAGLVLVPDQARLVTKKYMTNETLFHELSHSLGPGTINVGGRQTTVNAELK
                     DQYSASEEAKADVMGLHNILYMMKRGELPAAEREQALATYFVGIFRAVRFGTDEAHGR
                     GAAMQYDYLKEKGAFRWDEGADRFRVDYDRMEAGLSELLAELVRLQGNGDYAGTKAFF
                     DRHARLDERARAVLAKTAHIPTDIQPVYPMKV"
     gene            1599..2525
                     /locus_tag="VGB57_03560"
     CDS             1599..2525
                     /locus_tag="VGB57_03560"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEY0130457.1"
                     /translation="MDYQALVDEVLADVSAYKGQGNVASYIPALAEADPKKLGLALVL
                     RDGSSFVAGDADETFSIQSISYVFALCLALHHVKASLWNHVGREPAGSPVNSIVQLEM
                     EKGRPRNPMTSAGALVVCDQLIASAGGSDAAVGELVGFLRERCGDDKVTIDERIAMSE
                     SRAGAFNRSLAYFIAAFGNLKSPVEEVLSAYYRQCSVAMSCRQLARAALFLAFDGVDP
                     RTGDAVTIASRSRRINALMLTSGHYDNSGDFAFRVGLPGKSAMSGGLLAIVPGHGAAC
                     AWSPGLNAAGTSLAGAMALERLAERTGWSIFV"
     gene            2680..3210
                     /locus_tag="VGB57_03565"
     CDS             2680..3210
                     /locus_tag="VGB57_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130458.1"
                     /translation="MLTALLLAAAVPAAPACPIERAVYRLKGDPKFTAGFARQDRRDG
                     AVSDLVLWLNTPKRTYWFAFGSPNGYGGTYLAPELDPRVSAGLSDEEERDAAKKMELP
                     DPLSIEFDAFRADLGAFRAPPQSSDKPPALLFARGLGPALWYGPSTLAGNDSTAEQEG
                     MPIGLFVAAGCGGPPR"
BASE COUNT          568 a         1092 c         1108 g          518 t
ORIGIN      
        1 ccccatctac ctcaggcagc gatcgatcga caatcccacc atccgggccg agatcgcccg
       61 aagcgagcac ccgcggaagg cggccctgct cgacatgtac gacctgcatt tcggaccctg
      121 ggacacgctt gccgagaacc accccttcta cggcggcgag aagatgccgg cgggggcggg
      181 cttttacccg aaggacctga ccaaggcgga tttcgaagcc tatctggtcg ccaatcccgg
      241 tcagaaagag gcgctgacca gcggctacac cgtcgtcaaa aggcgcggcg accggctggt
      301 cgcggtgccc tacggcaccg aatacaggca atggctcgag ccggccgcga agctgctcga
      361 gcgggcggcg gcgcggacga gcaacgccag cctcgccaag ttcctgaacc ttcgcgccag
      421 ggccttcagg acgaacgatt attacgaatc ggagctggcc tggatggagg tcggcggaac
      481 cccgattgaa gtcgtgatcg gaccctacga ggtctacacc gacacgctct acgggacgaa
      541 gacggcgttc gaggccttca tcacggtgca ggatccgcgg gagagcgcgg ccctgacgaa
      601 gtacaaggcc ttgctgcccg acatggagcg caacctgccc gtcgaggacc gctacaagaa
      661 tttcaaccgg ccgttcgcct ctccgatcct cgtcgccgac caggtccatg gcggcggcga
      721 caatgtgccc ggggtgcaga ccatcgcttt caaccttccc aacgacgagc gcgtccgcga
      781 ggcgaagggg gcgaagaagg tgatcctcgc caacgtgctc ggtgccaaat acgaccggat
      841 cctggcgccg atggcggggc ttgtgctggt gcccgaccag gcccggctgg tgacgaagaa
      901 gtacatgacc aacgagaccc tgttccacga gctttcgcac agcctcgggc cggggacgat
      961 caacgtcggc gggcggcaga cgacggtgaa tgccgagctg aaggaccagt atagcgccag
     1021 cgaggaagcc aaggccgacg tcatggggct ccacaacatc ctctacatga tgaagcgcgg
     1081 cgagcttccg gcggccgagc gcgagcaggc gctggcgacc tatttcgtcg ggatcttccg
     1141 cgccgtgcgt ttcgggaccg acgaggcgca cggccgcggg gcggcgatgc aatacgacta
     1201 tctgaaggag aagggcgcgt tccgctggga cgagggggcg gaccgcttcc gggtcgatta
     1261 cgaccggatg gaggcggggc tgagcgagct tctggcggag ctggttcggc tccagggcaa
     1321 tggcgactat gccggcacca aggccttctt cgaccgccat gccaggctgg acgagcgggc
     1381 ccgggcggtg ctggccaaga cggcgcacat cccgaccgac atccagccgg tttatccgat
     1441 gaaggtgtag aaattcctcc ccggcatttg ccggggaggg ggaccaggcg gagcctgggg
     1501 gaggggttct tcgagccacc aagaacccct ccaccgcgct tcgcgcggtc cccctccccc
     1561 ggaaatccgg gggaggaatt taggaacgag gagagagaat ggattatcaa gccttagtgg
     1621 acgaggtgct cgccgacgtc tccgcctaca aggggcaggg gaatgtcgcc tcctatatcc
     1681 cggcccttgc cgaggccgat ccgaagaagc tcggcctggc cctcgtgctc cgggacgggt
     1741 cgagcttcgt cgcgggcgat gccgacgaga ccttctcgat ccagagcatc tcctacgtct
     1801 tcgccctctg cctcgccctg caccacgtca aagcctcgct gtggaaccat gtcgggcgcg
     1861 agccggcggg gtcgcccgtc aactcgatcg tgcagctcga gatggagaag gggcggccgc
     1921 gcaacccgat gaccagcgcc ggcgccctgg tcgtctgcga ccagctgatc gcctcggccg
     1981 gtgggtccga cgccgccgtg ggcgagctgg tcggctttct gcgcgagcgt tgcggcgacg
     2041 acaaggtaac gatcgacgag cgcatcgcca tgtcggaatc gcgggccggc gccttcaacc
     2101 ggagcctcgc ttatttcatc gccgccttcg gcaatctgaa gagcccggtg gaggaggtgc
     2161 tttcggctta ttaccggcaa tgctcggtgg cgatgagctg ccgccagctc gccagggccg
     2221 ccttgttcct ggccttcgac ggggtcgatc cgaggaccgg cgacgccgtc accatcgcct
     2281 cgcgctcgcg gcggatcaac gccctgatgc tgacgtcggg ccattacgac aatagcggcg
     2341 acttcgcctt ccgggtcggc ctgcccggca agagcgcgat gagcggcggc ctactcgcca
     2401 tcgtcccggg ccacggcgcc gcctgcgcct ggtcgcccgg cctcaacgct gcggggacgt
     2461 cgctggccgg ggcgatggcc ctggaaaggc tggcggaacg gacgggctgg tcgatcttcg
     2521 tctgataaaa tcctccccgc gatttcgcgg ggagggggac cgggcgaagc ccggtggggt
     2581 tctttggagg cggaagggcc cctccaccat gcttcgcatg gtccccctcc cctgcaaatg
     2641 caggggagga attttagttg tcgcatgtta ccaagcccca tgcttaccgc cctcctcctc
     2701 gccgccgcgg tgcctgcggc gcccgcctgt ccgatcgaaa gggccgtcta ccgcctgaaa
     2761 ggcgatccga agttcaccgc cggcttcgcc cgacaggacc ggcgcgacgg cgctgtctcg
     2821 gatctcgtcc tatggctgaa cacgcccaag cggacctact ggttcgcctt cggctccccc
     2881 aacggctatg gtggcaccta tctggctccg gagctcgatc cgcgcgtctc ggccgggctg
     2941 tcggacgagg aggagcggga cgcggcgaag aagatggaac tgcccgatcc gctcagcatc
     3001 gagttcgatg cctttcgcgc cgatctcggc gccttccggg cgccgcccca atccagcgac
     3061 aagcccccgg cgctgctctt cgcgcgcggc ctcggcccgg cgctctggta cggtccgtca
     3121 acacttgccg gcaacgactc cacggccgag caggagggga tgccgatcgg gctgttcgtc
     3181 gcggcgggat gcggcggccc gccgcgctga accatcaagg gaggaaagcc cgatgctgga
     3241 gccgatcgtg agcgaggcgc ttagccaatg cccggtcgac cgggcg
//
LOCUS       DASWNU010000180         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_214618, whole genome shotgun sequence.
ACCESSION   DASWNU010000180 DASWNU010000000
VERSION     DASWNU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214618"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGB57_03570"
     CDS             <1..499
                     /locus_tag="VGB57_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130459.1"
                     /translation="RFAPLWLLWLALCNLAVWLYFSLAWNGDALMWALLGLDSLALAA
                     WEAGHRARIPWLRDTWPPRLAAVAGGAMATGLALWSIFDGGLGLIGWLAWIGLVYFWY
                     RHVRPDLFMLAGGVLSLIVTVAAFLSRFMLDDGTGYLLIGLAVIAMSAGGAMWLNRLA
                     REQPE"
     gene            496..1566
                     /locus_tag="VGB57_03575"
     CDS             496..1566
                     /locus_tag="VGB57_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HEY0130460.1"
                     /translation="MSAQALWARLAAEGLVEGERPEPERPQSPWYVRTMLGIAGWIGA
                     LFLLGFVGAAFAFVMKDASAALFAGAACCGGALALYRSLRGQDFADQFALALSLAGQV
                     LMIVGLAELMKPGGPPLYFAIAAMQAGLALAVPNFLHRVLTAAGAALALALGIADLSL
                     HGLAAPLLCAALAWVWLEPARWARSGRLWRPIGYGLVLALLLVETFRLFGVAQWLDLA
                     RGTRGWMQSHGPLVGRVLTAALLVFVAVLISNREGHSTGSRTTLAAAAGAFLIGLFSL
                     GAPGFASALLILLLGFAAGNRLLMALGVLGLLGFASHFYYSLHSTLLVKSGLLAATGL
                     VLLAAHLILRRWPSGLAEPGHA"
     gene            1559..2038
                     /locus_tag="VGB57_03580"
     CDS             1559..2038
                     /locus_tag="VGB57_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HEY0130461.1"
                     /translation="MRKAVLLLAGLAILAFVNLGIYQRERLLTDGRIVLLRLSPVDPR
                     SLMQGDYMRLNFEVADQAFPAVASTPKADGHIVVLVDSGGIARFRRFSDGRPLAPGEI
                     ALRYRVRAGRPNFATDAYFFEEGRADRYAGAAYGEFRVGGDGDMILTGLRGPRLEPL"
     gene            2347..>2572
                     /locus_tag="VGB57_03585"
     CDS             2347..>2572
                     /locus_tag="VGB57_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130462.1"
                     /translation="MSFGSQVKLALALVPFTALIIFALLLAFQLAGAPVGGYDSMPML
                     AAVALIFALMIAAIQVAALGLIRLLPWQGPS"
BASE COUNT          289 a          921 c          854 g          508 t
ORIGIN      
        1 gcgcttcgcc ccgctctggc tgctctggct cgctttgtgc aacctcgccg tctggctcta
       61 tttctcactg gcctggaacg gcgatgcgct gatgtgggct ctgctcggcc tcgacagcct
      121 ggccctcgcc gcctgggaag cggggcaccg cgcccggatc ccctggctgc gggacacctg
      181 gccgccgcgc ctcgcggcgg tcgccggcgg agccatggcg accggcctcg ctttatggtc
      241 gatcttcgac gggggcctcg gcctcatcgg ctggctcgcc tggatcggcc tcgtctattt
      301 ctggtaccgg cacgtccgcc cggacctgtt catgctggcg ggcggcgtgc tgagcctgat
      361 cgtcaccgtc gccgccttcc tgtcgcggtt catgctggac gacggcaccg gctacctgct
      421 catcggcctc gccgtcatcg ccatgtcggc gggcggcgcg atgtggctca acaggcttgc
      481 gcgcgagcag cccgaatgag cgctcaggcg ctgtgggccc gcctcgcggc cgagggccta
      541 gtcgagggcg agcggccgga gccggagcgg ccgcaatcgc cctggtacgt tcggacaatg
      601 ctcggaatcg ccggctggat cggagcgctc ttcctgctcg gcttcgtcgg cgcggccttc
      661 gccttcgtga tgaaggacgc cagcgccgcc ctgttcgccg gagcggcctg ctgcggcggc
      721 gccctcgctt tgtaccgcag tctgcgcggg caggatttcg ccgaccagtt cgctttggct
      781 ttgagcctcg ccgggcaggt tctgatgatc gtcggcctcg ccgagctcat gaagcccggc
      841 ggtccgccgc tctatttcgc tatcgccgcg atgcaggccg ggctggcgct ggcggtaccg
      901 aacttcctcc accgcgtgct gaccgccgcc ggcgcggcgc tggcgctggc gctcgggatc
      961 gccgacctgt cgctgcacgg cctggccgct cccttgcttt gcgccgcgct cgcctgggtc
     1021 tggctcgagc ccgcgagatg ggcgcggagc ggccggctgt ggcgtccgat cggttacggg
     1081 ctggtgctgg ccctcctgct cgtcgagacc ttccgcctgt tcggagtggc gcagtggctc
     1141 gacctcgccc gcgggacgcg gggctggatg cagagccacg ggcctctggt cgggcgagtc
     1201 ctgacggcgg ctctcctcgt cttcgttgcg gtccttatct cgaaccgcga gggccattcg
     1261 acggggagcc gaaccacatt ggccgcggcg gccggcgcct tcctcatcgg tctgttctcg
     1321 ctcggcgctc cgggcttcgc ctcggcgctg ctgatcctgc tgctcggctt cgccgccggg
     1381 aaccggcttc tcatggccct gggcgtgctc ggcctgctcg gcttcgccag ccatttctat
     1441 tacagcctgc actcgaccct gctcgtcaaa tcggggcttc tggcggcgac cggcctcgtc
     1501 ttgctcgccg cccatctcat ccttcggcgc tggccgtccg gactcgcgga gcccggccat
     1561 gcgtaaggcc gtcctcctcc tcgccgggct tgccatcctc gccttcgtca atctggggat
     1621 ctaccagcgc gagcggcttc tgaccgacgg caggatcgtt ctcctgcggc tgagccccgt
     1681 cgatccgcgc tcgctgatgc agggcgatta tatgcggctc aacttcgagg tcgccgacca
     1741 ggccttcccg gcggtcgcct ccactccgaa ggcggacggg catatcgtcg tcctcgtcga
     1801 ctccggggga atcgcccggt tccggcgctt tagcgacggc cgcccgctcg cgcccggcga
     1861 gatcgcgctc cgctaccggg tccgcgccgg ccggccgaac ttcgccaccg acgcctattt
     1921 cttcgaggaa gggcgggcgg atcgttatgc cggcgccgcc tatggcgaat tccgggtcgg
     1981 cggcgacggc gacatgatcc tgaccggcct gcgcggcccc cggctggagc ccttgtgacc
     2041 ggcgccgcct agggtccgga ctcaataagg atcgcacctc gatcgggaga tccgtcatcc
     2101 cggcgcaggc cgggatccat gaacacggaa aattgcagtg acaggctcga ccggattcgc
     2161 ctctttttcg aagggctgtg ttcatggatc ccgaccttcg tcgggatgac gctggaatcc
     2221 ctcgaaaatc taccggagct aattgagtgc ggtccctggg ctggccgggt cgctgctatt
     2281 ccgctttgag tccgcggcgg atatcgatgc agccgaagga gacgagccat catcagcctc
     2341 agacaggtgt cgttcgggtc gcaggtgaag ctggcgctcg cccttgttcc gttcaccgcc
     2401 ctgatcatct tcgcgctgct gctggccttc cagctcgccg gcgcgcccgt cggcggctac
     2461 gacagcatgc cgatgctcgc cgccgtggct ttgatcttcg ctctgatgat cgcggccatt
     2521 caggtcgccg ctctggggct catccgcctc cttccctggc agggaccgtc gc
//
LOCUS       DASWNU010000181         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_474658, whole genome shotgun sequence.
ACCESSION   DASWNU010000181 DASWNU010000000
VERSION     DASWNU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474658"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGB57_03590"
     CDS             complement(<1..183)
                     /locus_tag="VGB57_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130463.1"
                     /translation="MKNALPLLALLLATACTTTAGRNDVTFSDPPAPAEVSAPAAADP
                     AAEDSRLLAFLDAAFDE"
     gene            complement(287..1297)
                     /locus_tag="VGB57_03595"
     CDS             complement(287..1297)
                     /locus_tag="VGB57_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0130464.1"
                     /translation="MLLDMERSSTDLLDVLAFVRVVETGAFSRAGERMGMSKSILSRR
                     VARLEEQLGARLLTRTAQGAQPTEVGQAYFERASNILADLEAAEEVVAEAVTQVAGPV
                     RLSAPLSFGVQHLAPALAEFAQLNPRVELDISFDDRSVDLAAGGFDLAVRIGSLKDSS
                     LVARRIAPVRKVATASPAYLDARGRPQHPRDLAGHDILLYANEQWRFRVGGKWEYVRG
                     RPRLRADNGEMLRAAAEAGLGICILPSFIAAPGFERGTLEPVLRGFPMEEGALHAVMP
                     PGRATTARVRALVDFLVERFGPEPEWDPCWLAGHEERSSSPAFAGEGDHAKHGGGALQ
                     TP"
     gene            1373..>1883
                     /locus_tag="VGB57_03600"
     CDS             1373..>1883
                     /locus_tag="VGB57_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0130465.1"
                     /translation="MTNVLIIDSAATGEASVTRRLTAELESILRGRGPVRIVHRDVGT
                     HTIPHLTAETTPAIRGAEAESEAARDALALSDALIAELKAADIVVIGAPMYNFGIAST
                     LKAWFDHVLRAGITFRYSEAGPEGLLKGKKAIVIESRAGLYSTGPSAPMDSQEPHLRT
                     LLGFMGLDDM"
BASE COUNT          314 a          693 c          611 g          265 t
ORIGIN      
        1 ctcgtcgaag gctgcgtcga ggaaggcgag gagacgcgag tcctcggcgg cgggatcggc
       61 ggcggccggc gcggaaactt cggcgggagc gggcgggtcg ctgaaggtga cgtcgttccg
      121 cccggcggtg gtggtgcagg cggtggcgag gagaagggcg aggagcggga gggcgttttt
      181 catgaggtcg acgctttagc gcctcgtcga gccgcgtcca gcctctctaa attcctcccc
      241 cggatttccg ggggaggggg accgcgcgaa gcgcggtgga ggggttctac ggagtctgaa
      301 gagcccctcc accatgcttc gcatggtccc cctcccctgc aaatgcaggg gaggaacttc
      361 tctcctcatg ccccgccagc cagcacgggt cccactccgg ctccggcccg aaccgctcga
      421 ccaggaaatc gaccagcgcc cgcacccgcg ccgtcgtcgc ccgccccggc ggcatcaccg
      481 cgtggagcgc gccttcctcc atcgggaaac cgcgaagcac cggctccagc gttccacgct
      541 cgaagccggg cgcggcgatg aagcttggaa ggatgcagat cccgagcccc gcttccgccg
      601 ccgccctaag catctcgcca ttgtcggccc tcagcctcgg ccgaccgcgc acatattccc
      661 atttgccgcc gacccggaat cgccactgct cgttggcgta gagcaggatg tcgtggcccg
      721 cgaggtcgcg cggatgctgc ggccgccccc gggcgtcgag ataggcgggg ctggccgtcg
      781 ccactttccg caccggcgcg atccggcgcg ccaccagcga cgaatccttc agcgagccga
      841 tccgcaccgc aaggtcgaag cccccggcgg cgagatcgac ggaccggtcg tcgaacgaga
      901 tgtcgagctc gacccgagga ttcaactgcg cgaactcggc cagcgccggc gcgagatgct
      961 gaaccccgaa ggagagcggc gccgacagcc gcaccggccc ggccacctgg gtcaccgcct
     1021 ccgccaccac ctcctccgcc gcctcgagat cggcgaggat gttggacgcc cgctcgaaat
     1081 aggcctggcc gacctcggtc ggctgagccc cctgcgcggt ccgggtcagg agccgggcgc
     1141 ccaattgctc ctccagccgc gccacccgcc ggctgaggat cgacttggac atccccatcc
     1201 gctcgccggc gcggctgaac gcgcccgtct ccaccacccg gacgaaggct aggacatcga
     1261 gcaggtcagt cgaggagcgt tccatatcga gcaacaccag aatgcgccaa tggcggctac
     1321 aagcaacgct gctaccggct tacatgtctc cttgcaagca gtggaggaag acatgaccaa
     1381 cgttctcatc atcgacagcg ccgccaccgg cgaggcctcc gtcactcgcc ggctgaccgc
     1441 cgagctcgag tcgatcctcc gcggccgggg cccggtcagg atcgtccatc gcgacgtcgg
     1501 cacccacacc atcccgcatc tgaccgccga gaccacgccc gccatccgcg gcgccgaggc
     1561 cgagagcgaa gcggcccgcg acgccctcgc cctttccgac gcgcttatcg cggagctgaa
     1621 ggccgccgac atcgtcgtca tcggcgcccc gatgtacaat ttcgggatcg cctcgacgct
     1681 caaggcctgg ttcgaccacg tccttcgcgc cggaatcacc ttccgctatt cggaagccgg
     1741 acccgagggg ctgctcaagg gcaagaaggc gatcgtgatc gagagccgcg ccggcctcta
     1801 cagcaccggc ccctcggcgc cgatggacag ccaggagccg catctgcgca cgttgctcgg
     1861 cttcatgggc ctggacgaca tga
//
LOCUS       DASWNU010000182         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_189035, whole genome shotgun sequence.
ACCESSION   DASWNU010000182 DASWNU010000000
VERSION     DASWNU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189035"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VGB57_03605"
     CDS             <1..1334
                     /locus_tag="VGB57_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0130466.1"
                     /translation="DTYRQDSRNYALFTHNIIHITDNLALTIGLRYTNEKKELNATFG
                     NDNVACQQQQAALLPLLANPGLAPLAAGLIGLTCQGNSTAELNGVSISDDRSEDEFTG
                     TTILSFKPSRDLMVYGSWSRGYKAGGFNLDRSALKAPVLPFSATPGGAQGLVGNLQFD
                     PEINTAIELGAKFSRGPLTLNAVLFRQSFKNFQLNTFNGTVFLVQTVNGCGIDLAGGD
                     RDQSILPGASNYVAPPAANPTANPAAATGACPAGDVGYGVRSEGVELESALRVRRDLN
                     MTLGLTYANTRYRSQLVGNESGAPLDPALRMLPGDNLSNAPEVVVTGSLAWTPRLGNS
                     GLSGLFYVDGRLTDGFNTGSDLFPQKEQDSYAVFNARIGVRGADERWAIEAWAMNVFN
                     VGYTQVAFNSPFQEGARTAAFADPQFPGGRQIFSAFLAEPRTFGLTGRFRF"
     gene            complement(1506..>1960)
                     /locus_tag="VGB57_03610"
     CDS             complement(1506..>1960)
                     /locus_tag="VGB57_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0130467.1"
                     /translation="AQAVIHLHTPHGQAVSAHNEGLLPLTQTAMLIRDEVAYHDYEGV
                     AVDLDERERIVADLGDKGAMLLRNHGTLTVGETIGEAFVRMYFLERACEAQILALSAG
                     EGNLNNPPQGSPEVTAVQGKIGLKLAAGALAWPALLRKAYRLDPGFAT"
BASE COUNT          322 a          704 c          615 g          319 t
ORIGIN      
        1 tcgataccta tcgccaggac agccgcaatt acgcgctgtt cacccacaac atcatccata
       61 tcaccgacaa tctggccctg acgatcggcc ttcgctacac gaacgagaag aaggagctca
      121 acgcgacctt cggcaacgac aacgtcgcct gccagcagca gcaggcggcg ctgctcccgc
      181 tccttgccaa tcccggcctg gcgccgctcg cggccggcct gatcggcctc acctgccagg
      241 gcaattcgac cgccgaattg aacggggtct ccatctccga cgatcgcagc gaggacgagt
      301 tcaccggcac caccatcctc tcgttcaagc cgagccggga tctgatggtc tatgggagct
      361 ggtcgcgcgg ctacaaagcg ggcggcttca acctcgaccg atccgccctc aaggcgccgg
      421 tgctgccgtt ctcggcgact cccggcggcg cccaggggct ggtcggaaat ctccagttcg
      481 atccggagat caacaccgcc atcgagctcg gcgccaaatt ctcgcgcgga ccgctcactc
      541 tcaacgccgt gctgttccgc cagagcttca agaacttcca gctgaacacc ttcaacggca
      601 ccgtcttcct cgtgcagacg gtgaacggct gcggaatcga tctggccggc ggcgaccgcg
      661 accagagcat cctccccggc gcctcgaact acgtcgctcc gccggcggcg aacccgaccg
      721 ccaatcccgc cgcggcgacc ggcgcctgcc cggccggcga tgtcggctac ggagtccgtt
      781 cggaaggcgt cgagctggaa agcgcactgc gggtccgccg cgacctcaac atgaccctcg
      841 gcctcaccta cgccaacacc cgctaccggt cgcagctggt cggcaacgag agcggcgctc
      901 cgctcgatcc ggcgctgcgc atgctgcccg gcgacaatct ctccaacgcg ccggaggtcg
      961 tcgtcaccgg atcgctggcc tggactccgc gcctcggcaa ttcggggctg agcggcctct
     1021 tctatgtcga cggccgactc accgacggct tcaacaccgg ctcggacctg ttcccgcaga
     1081 aggagcagga cagctacgcc gtgttcaacg cccggatcgg cgttcgcggc gccgacgagc
     1141 gctgggcgat cgaggcctgg gcgatgaacg tcttcaatgt cggctacacc caggtcgcgt
     1201 tcaactcgcc cttccaggaa ggcgcccgca cggcggcgtt cgccgacccg caattcccgg
     1261 ggggacggca gatcttctcc gccttcctcg ccgagccgcg caccttcgga ctgaccgggc
     1321 ggttcaggtt ctgattctgc ctccccatcg cgaagcgatg gggaggggga ccctgcgaag
     1381 cagggtggag gggtaattgg cgcgagagct tgagggatct cgcgcgattc cccccggtcc
     1441 gcgccttcta cccctccacc acacttcgtg tggtccccct ccccatctgg cgatggggag
     1501 gcagttcaag tcgcgaagcc gggatccagc cggtaggcct tgcgcagcag cgccggccag
     1561 gcgagcgccc cggcggcgag cttcaggccg atcttgccct gcacggcggt cacttccggc
     1621 gagccctggg gcgggttgtt gagattgccc tcgccggcgg acaaagcgag gatctgcgcc
     1681 tcgcaggcgc gctccaggaa atacatcctg acgaaggcct cgccgatcgt ctcgccgacg
     1741 gtcagcgtgc cgtggttcct gagcagcatc gcccccttgt ccccgagatc ggcgacgatc
     1801 cgctcgcgct cgtcgaggtc gacggcgact ccctcataat cgtgataggc gacctcgtcg
     1861 cggatcagca tcgccgtctg ggtgagcggc agaagcccct cgttgtgggc ggagaccgcc
     1921 tggccgtggg gggtgtgaag gtggatgacg gcctgggcgt
//
LOCUS       DASWNU010000183         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_422383, whole genome shotgun sequence.
ACCESSION   DASWNU010000183 DASWNU010000000
VERSION     DASWNU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422383"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1829)
                     /locus_tag="VGB57_03615"
     CDS             complement(<1..>1829)
                     /locus_tag="VGB57_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0130468.1"
                     /translation="LPEGMTIGVGSDDSLFIGESIKKVWHTLAEAAVLVVAVIFLFLG
                     SWRATLIPAVTVPLCLLGSFVILWIFGFSINLLTLLAMVLAIGIVVDDAIVVLENVYH
                     RIEEGEPPLAAAFEGTRQVGFAIVSTTLVVCAVFVPIMFIAGQTGLLFRELAAAMIGA
                     VAFSGFLALSLAPMLCSKLLRYQERGRFAGWVDERFRRLERFYSGRLDAAIRKPVLPL
                     ALVGIFLAGAGALFLTMQSELVPAEDVGILSANVNAPEGTGYAQMDRYMIDAQGKLLP
                     LVDKGAVRAIITRTPGGFGASDDFNSGNFVVFLKPWEEREKTTQEVVQEVNKRLGQLP
                     AVRGNAQVRSSIGGRGRGQPVSFVIAGSTYAELARARDRILAAAAANPGLINLDSDYK
                     ETKPQLRIAVDTARAGDLGVSVEDVSNALQSLLGSRRVSTYVDRGEEYRVVVQAEAGN
                     RATQADLQTIHVRNRSGGVVPLASLVTVTDTAGARDLGRYNKMRAITLSGGLAPGYSL
                     GQALEFLEAQAAQSPEVVAIGYRGESQAFKEAGGSIWLVFGLTILIIYLLLAAQFESF
                     VHPAVIITTVPLAVAGGVIGLAVMSQTMNLYSQIGVIMLVGLA"
BASE COUNT          327 a          586 c          653 g          263 t
ORIGIN      
        1 cggcaaggcc gaccagcatg atcacgccga tctggctgta gaggttcatc gtctggctca
       61 tcacggcgag gccgatcacg ccccccgcca ctgcgagcgg cacggtggtg atgatcacgg
      121 ccgggtggac gaagctttcg aattgggcgg cgagaagcag atagatgatc aggatggtga
      181 ggccgaagac cagccagatc gagccgcccg cttccttgaa ggcctggctc tcgccgcgat
      241 agccgatcgc gaccacctcc ggcgactggg cggcctgcgc ctcgaggaac tccagcgcct
      301 ggccgagcga atagccgggg gcgaggccgc cggagagggt gatcgcccgc atcttgttgt
      361 agcggccgag gtcgcgggct ccggccgtgt cggtgaccgt caccaggctg gcgagcggga
      421 ccactccgcc ggagcggttg cggacgtgga tggtctgtag atcggcctgc gtcgcgcggt
      481 tgccggcctc ggcctggacg accacccgat attcctcgcc gcgatcgaca taggtggaga
      541 cccggcggga gccgagcagc gactgcagag cgttggagac gtcctcgacc gagactccga
      601 gatcgcccgc gcgggcggtg tcgacggcga tccgaagctg cggcttggtt tccttatagt
      661 cggaatcgag attgatgagg ccgggattgg cggccgcggc ggcgaggatg cggtcgcggg
      721 cgcgggcaag ctcggcatag gtggagccgg cgatgacgaa gctgaccggc tgcccccggc
      781 cgcggccgcc gatcgacgag cggacctggg cgttgccgcg gaccgcggga agctggccga
      841 gccgtttgtt gacctcctgg acgacctcct gggtggtctt ctcgcgctcc tcccagggct
      901 tgaggaagac gacgaaattg ccgctgttga aatcgtcgct ggcgccgaag ccgcccggcg
      961 tccgggtgat gatggcccgg accgcgccct tgtcgacgag cggcagcagc ttgccctgag
     1021 cgtcgatcat gtagcggtcc atctgcgcgt agcccgtgcc ttcgggcgcg ttgacgttgg
     1081 ccgagaggat cccgacgtcc tcggccggca ccagctccga ctgcatggtg aggaagagtg
     1141 cgccggcgcc ggccaggaag atcccgacca gggccaaagg aaggactggc ttgcggatcg
     1201 cggcgtcgag gcggccggag tagaagcgct cgagccggcg gaagcgctcg tccacccagc
     1261 cggcgaaacg gccgcgctcc tgatagcgga gcagcttcga gcagagcatc ggagcaaggc
     1321 tgagcgccag gaagccggaa aaggcgacgg cgccgatcat cgcggcggcg agctcgcgga
     1381 acagcaggcc ggtctgaccg gcgatgaaca tgatcggcac gaacaccgcg cagacgacga
     1441 gcgtggtcga gacgatcgcg aagccgacct gcctcgtccc ttcgaaggcg gcggccagcg
     1501 gcggctcgcc ctcctcgatc cggtgataga cgttctcgag cacgacgatg gcatcgtcga
     1561 cgacgatgcc gatcgcgagc accatcgcca gcaaggtcag caggttgatc gagaagccga
     1621 agatccagag gatgacgaag cttccgagca ggcagagcgg caccgtcacc gccgggatca
     1681 gggtcgcgcg ccaactgccc aggaacagga agatcaccgc caccaccagc accgccgcct
     1741 cggcgagcgt gtgccagacc ttcttgatcg attccccgat gaacagcgaa tcgtccgacc
     1801 cgacgccgat ggtcattcct tccggcagt
//
LOCUS       DASWNU010000184         8265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_395806, whole genome shotgun sequence.
ACCESSION   DASWNU010000184 DASWNU010000000
VERSION     DASWNU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395806"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGB57_03620"
     CDS             <1..384
                     /locus_tag="VGB57_03620"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0130469.1"
                     /translation="SGNLWATLMANDTDVDSSPIGRRILSVDTAGTQGSVLFDAATKT
                     LTYSAAGIDLAPGETITDTFTYTVSDSVSGSQPDTATVTVTVIGAAAMSAFLAEGEAE
                     VFGADFAGYALVHPEQLMIADMPIP"
     gene            480..1328
                     /locus_tag="VGB57_03625"
     CDS             480..1328
                     /locus_tag="VGB57_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY0130470.1"
                     /translation="MLIRRLAPYAAHLRLPFQLTLAPIFLWGALLSDGGWDMGTSAAF
                     VALHLSLYPAATAFNAVYDRDSGPVATLAQPPEVPPHLLRFALALALIGALPAFAAGA
                     PFLLAYVLIAGWTAAYSHPRTRWKASPWQSAAAIALGQGALGFLAGWIATAAPDAPAW
                     PFAAGAAGAALTALGLYPATMAFQVAEDRLRGDRTLAVALGPERALRLGSLCLLLGGA
                     VTAWLVARRFGPSDAALIVLGYLALVLAQERLARRPDYRRAMRLLNRATACFLLFLVL
                     EAIGRG"
     gene            1383..2576
                     /locus_tag="VGB57_03630"
     CDS             1383..2576
                     /locus_tag="VGB57_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130471.1"
                     /translation="MGEAGGIHHRLVAEIEDEFEGSLGLSVVPIVPVRGGIAWRARLP
                     DSTLRAALIVAGTYFLIFVALPLLALGGGWPPASSALWPLAILQLYGMFWCGTSTAAT
                     RSTSVSILRIIKWEIVPFLSEASAERLLEILHKPSYHRSTQLRIAWTIGVAAAAFAAF
                     LVRQDLARAGVDAPSTIALIWWSVGWALLYATAAKVVLVSRICIWFGDVLSDQPERLS
                     PLNPAQSPLVTSLLALTKRMLLFWAGMALAIALVIPFALAVWGYGEPFAGPAWHSRVG
                     EAVADVRRLHPSAFGFVLVHFVLTTIMSIGGGTLILIKSDAAARSAARRATARVLRAL
                     DERANAIADAPLTEESLKRLAELKALHSDIAARGSHRGLIVGAVGLLLPFAPLLALLL
                     SVLSG"
     gene            complement(2586..3251)
                     /gene="purQ"
                     /locus_tag="VGB57_03635"
     CDS             complement(2586..3251)
                     /gene="purQ"
                     /locus_tag="VGB57_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0130472.1"
                     /translation="MKSAVIVFPGSNCDRDMAVALEQVTGNRPAMVWHRETGLPEGLD
                     LIAIPGGFSYGDYLRSGAMAAQSPVMRAVAEAAERGVAVLGVCNGFQILTEARLLPGA
                     LMRNAAIRFVSRPVALRVENSQSAFTSRYEAGEEIVIPVAHHDGNYQADPETLNRLEG
                     EGRIAFRYAGTCNGSARNIAGIVSEAGNVLGMMPHPERAIEPAHGRTDGRRLFEGLIE
                     ALA"
     gene            complement(3248..3499)
                     /gene="purS"
                     /locus_tag="VGB57_03640"
     CDS             complement(3248..3499)
                     /gene="purS"
                     /locus_tag="VGB57_03640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY0130473.1"
                     /translation="MKTRVYVTLKNGVLDPQGKAIHHALEGLGFAGVNDVRAGKLIEL
                     DLAEGVGDEEVEAMCRKLLANTVIENFRIEREAAEGQAA"
     gene            3717..4052
                     /gene="erpA"
                     /locus_tag="VGB57_03645"
     CDS             3717..4052
                     /gene="erpA"
                     /locus_tag="VGB57_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY0130474.1"
                     /translation="MTEPSEIALTPAAAARVAAIARRQGKPAILRLSVEGGGCAGFSY
                     RFGLADEVESDDLVAGESGVTLVVDPMTHDLVKGGAVDYVESLGGAAFQVRNPNAASG
                     CGCGSSFSV"
     gene            4175..4960
                     /gene="xth"
                     /locus_tag="VGB57_03650"
     CDS             4175..4960
                     /gene="xth"
                     /locus_tag="VGB57_03650"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0130475.1"
                     /translation="MKIVTYNLNGIRARLPRLIEYLQEEKPDALCLQEIKCADEAFPI
                     EDVRAAGYDGVWHGQKGFNGVAILTPVDAGPPELRRTGLPGDPDDTHSRYIEAEVGGV
                     VIGSIYLPNGNPVGTGKFAYKLAWMERLREHALALLAEERPVVLAGDWNVIPTDSHDD
                     VFSVRAMAHDALMQPESRAAFRRILHSGWTDSIRARHPDGAVYTFWDYTAGCWQRDAG
                     FRIDHLLLSPQAADRMLDAGVDKSHRGREKASDHAPTWVRLEE"
     gene            4983..6848
                     /locus_tag="VGB57_03655"
     CDS             4983..6848
                     /locus_tag="VGB57_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY0130476.1"
                     /translation="MTVTILEGMPMQPWTRRHFLGTAGAVAALQLLPGCATRRPAGSG
                     GSVDALLSRMAESILAEYPENATSLGLDKDARAGLKSRLTDRSLHGRARLAGSAADRL
                     RRLQAADRTGPGPAEQIHLGVAETAHRLAVEGFRFPYGDVVTLNQNYSYRNAPYVVAQ
                     NTGAFVEVPDFLDSSHSVANAADAEAYLDRLEAYAGALEGETERLAHDRGIGVVAPDF
                     LLDKTLKQMKGARSQPLGEWGVVTSLERRTAQVPGDWRGRALALARDRVAPAMDRQIA
                     ELTRHRARADSRAGVWKLPDGEDYYAWALRAATTSSLTPAEVHKMGLDQLAELQGRME
                     PLLRAQGLTRGTVGQRMTALGNDPKHLFPNTDAGRAQILEFVNGKVADIRTRLPRAFA
                     TLVPGRLIIKRVPQEIEAGAPGGYAAAGTIDGSVPGNYYINLRDTAMWPRFSLATLTY
                     HEGIPGHIWQGEYTYKLPLLRSLLAFNAYSEGWALYAEQLADELGAYEGDPLGELGYL
                     QSLSFRACRLVVDSGLHAQRWTRARAIQWFAETNGSTVEEVQGEVDRYCAWPGQACGY
                     KVGHSEIVRLREKARAALGGKFDLRTFDDALVLGGNVPLTLLEGVVDRYVSTRSG"
     gene            complement(7711..>8265)
                     /locus_tag="VGB57_03660"
     CDS             complement(7711..>8265)
                     /locus_tag="VGB57_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0130477.1"
                     /translation="RVGENISYYILTVFSLTFLVDVAAESRSQALQALLIGAAVQFFA
                     IPLFARLSDRIGRRPVYAFGGLGLAAFSFAFFPMLASGDPALIVLAVVAGLVLHSAMY
                     GPQAAFITELFPTRIRYSGVSIAYQLTSILAGSLAPIVALWLYREYNSALPVAIYVGV
                     ACFVSGVTALLAKETKGVELSAIR"
BASE COUNT         1232 a         2931 c         2815 g         1287 t
ORIGIN      
        1 tccggcaacc tctgggcgac gctgatggcg aacgacaccg acgtcgattc gagcccgatc
       61 ggccgccgca tcctgtcggt cgacacggcc ggcacccagg gcagcgtcct gttcgacgct
      121 gcgaccaaga cgctgaccta ttcggcggcc ggtatcgatc ttgcgccggg cgagacgatc
      181 acagacacat tcacctacac ggtgtcggac agcgtctccg gaagccagcc ggacacggcg
      241 acggtgacgg tgacggtgat cggcgccgcc gcgatgtccg ccttcctcgc cgagggcgag
      301 gcggaggtct tcggcgcgga cttcgccgga tacgcgctgg tgcacccgga gcagctgatg
      361 atcgccgata tgccgatccc ctgatcgggc gcggcacctg ccgcgggaag gccgctcccg
      421 gcgacggggg cggcctttcg cttgcgcggc ggcggcgcgc cgtgcgagca gcgcgggcca
      481 tgctgatccg ccgtctcgcg ccctatgccg cgcatctccg cctgcccttc cagctgacgc
      541 tggcgcctat cttcctttgg ggcgcgctgc tttcggacgg cggctgggac atgggcacga
      601 gcgcggcctt cgtcgcgctg cacctgtccc tctatcccgc cgccaccgcc ttcaacgccg
      661 tctacgaccg ggattcgggc ccggtcgcga cgctcgccca gcctccggag gtcccgccgc
      721 atctgctccg cttcgcgctt gcgctggcgc tgatcggcgc ccttcccgcg ttcgcagccg
      781 gcgcgccctt cctgctggcc tatgtcctga tcgccgggtg gaccgccgcc tacagccacc
      841 cgcgcacccg ctggaaggcg agcccctggc agagcgcggc cgccatcgcc ctcggtcagg
      901 gcgcgctcgg cttcctggcc ggctggatcg ccaccgccgc gccggatgcg ccggcatggc
      961 cgttcgcggc aggagccgcc ggcgcggcgc tgaccgcgct cggcctctac cccgcgacca
     1021 tggccttcca ggtggcggaa gaccgcctcc gcggcgaccg caccctcgcc gtcgcgctgg
     1081 ggccggagcg cgcgctgcgc ctcggcagcc tttgcctgct gctcggcggc gccgtcaccg
     1141 cctggctggt cgcgcgccgc ttcggcccgt cggacgccgc cctaatcgtg ctcggctacc
     1201 ttgcgctggt cctcgcccag gagcgcctcg cccgccgccc agactaccgc cgcgcgatgc
     1261 gcctgctcaa ccgtgccacc gcctgcttcc tcctcttcct cgtcctggag gcgatcggcc
     1321 gcggctagcg cggcgccgcg cgcgtccttg gcaccatgcc ggcgaaagcc tagactcgcg
     1381 ccatggggga ggcgggcggc atccatcacc ggctggtggc cgagatcgaa gacgagttcg
     1441 agggatcgct cgggctgagc gtcgtgccga tcgtgccggt gcggggcggc atcgcctggc
     1501 gcgccaggct gcccgactcc accctgcgcg cggccctgat cgtggccggc acctatttcc
     1561 tgatcttcgt cgcgctgccg ctgctggcgc tgggaggggg ctggccgccg gcgtcgagcg
     1621 cgctgtggcc cctggcgatc cttcagctct acggcatgtt ctggtgcggc acctccaccg
     1681 ccgcgacccg ctcgacgtcg gtatcgatcc tcaggatcat caagtgggag atcgtaccct
     1741 tcctctccga ggcgagcgcg gagcgccttc tcgaaatcct ccacaagccc agctaccaca
     1801 gaagcaccca gctccgcatc gcttggacga tcggcgtggc cgcagcggca ttcgccgcct
     1861 ttctcgtccg gcaggacctg gcgcgggccg gcgtagacgc accctcgacg atcgccctga
     1921 tctggtggtc ggtcgggtgg gctttgcttt atgccaccgc cgccaaggtc gtcctggtct
     1981 cgcgcatctg catctggttc ggcgacgtgc tctcggacca gccggagcgc ctgtcgccgc
     2041 tcaatcccgc acaatcgccg ctcgtcacca gcctgctcgc gctcaccaag cggatgctgc
     2101 tgttctgggc gggcatggcg ctggcgatcg cgctcgtcat cccgttcgcg ctggcggtct
     2161 gggggtatgg cgagcccttc gccggcccgg cctggcatag cagggtcggg gaggccgtcg
     2221 ccgacgtccg gcgtcttcac ccttccgctt tcggcttcgt cctcgtccac ttcgtgctca
     2281 ccacgatcat gtcgatcggc ggcgggactt tgatcctgat caagagcgac gcggcggcac
     2341 gttcggcggc gcggcgcgcg acggcccggg tcctgcgcgc gctcgatgag cgggccaacg
     2401 ccatcgccga cgcccctctc accgaagagt cgctgaagcg cctcgccgaa ctcaaggcgc
     2461 ttcattcgga tatcgccgcc cgcggctccc accgcggcct gatcgtcgga gccgtcggcc
     2521 tgctgcttcc gttcgcgccg ttgctcgctt tgctgctcag cgtcctcagc ggctgagggc
     2581 gagcctcagg cgagcgcctc gatcagccct tcgaacaggc gccgcccgtc ggtgcggccg
     2641 tgggcgggct cgatggcgcg ctcggggtgg ggcatcatgc ccagcacgtt gccggcctcg
     2701 ctgacgatgc cggcgatgtt gcgcgccgac ccgttgcatg tcccggcata gcggaaggcg
     2761 atgcggccct cgccttccag gcggttgagc gtctccgggt cggcctggta attgccgtcg
     2821 tgatgggcga ccgggatgac gatctcctcg cccgcctcgt agcgcgaagt gaaggcggac
     2881 tggctgttct cgacgcgcag cgccaccggg cgggaaacga agcggatcgc ggcgttgcgc
     2941 atcagcgcac cggggagcag gcgcgcctcg gtcaggatct ggaagccgtt gcacacgccg
     3001 agcaccgcga cgccgcgctc cgccgcctcg gcgaccgccc gcatcaccgg cgattgcgcc
     3061 gccatcgcgc cggagcgcag atagtcgccg taggagaagc cgccggggat ggcgatcagg
     3121 tcgagccctt cgggaagccc ggtctcgcgg tgccagacca tcgcgggcct gttgccggtc
     3181 acctgctcga gcgcgaccgc catgtcgcga tcgcaattgg agccggggaa gacgatgacc
     3241 gcgctcttca cgccgcctgt ccttccgccg cctcgcgctc gatccggaaa ttctcgatga
     3301 cggtgttggc gagcagcttt cggcacatcg cctccacctc ctcgtcgccg acgccctcgg
     3361 ccaggtcgag ctcgatcagc ttgccggcgc gcacgtcgtt gaccccggcg aagccgagcc
     3421 cctcgagcgc atggtggatg gccttgccct gcggatcgag cacgccgttc ttcaaggtga
     3481 cgtagacgcg ggtcttcatg gacatttctc gtggcgggaa gatggggccc tatgccgtca
     3541 aagcaagcgc cggcacaagg gccgccgcgc cgccgcgcca ccgcgccacc gcgtcatccc
     3601 ggcggaagcc gggatctcgg ggagacccgc cgtgacttct cgtcccgagg tcccggcttt
     3661 cgccgggatg acggggggcg cttgtggcca acccgcccag gccctatctt ccccctatga
     3721 ccgagccctc cgaaatcgca ctcacccccg ccgcggcggc gcgagtcgcc gcgatcgccc
     3781 ggcgccaggg caagccggcg atccttaggc tcagcgtcga gggcggcggc tgcgccggct
     3841 tcagctaccg cttcggcctc gccgacgagg tggagtcgga cgatctcgtc gccggcgaga
     3901 gcggggtcac gctggtcgtc gacccgatga cccacgatct ggtgaagggc ggcgcggtcg
     3961 attacgtcga atcgctcggc ggagcagctt tccaggtcag gaacccgaat gcggcctccg
     4021 gctgcggctg cggttcgagc ttcagcgtct aattgtcatt ccggcgaacg ccggaatcca
     4081 tgaacaccgt gtcacttcgg ataaatccgt gttcatggat cccggaacaa gtccgggatg
     4141 acggccttcg tgcgagccgc taagccgcct ccccatgaag atcgtcacct acaacctcaa
     4201 cggaatccgg gcccggctgc cgcggctcat cgaatatctg caggaggaga agcccgacgc
     4261 gctctgcctg caggagatca aatgcgccga tgaggccttt ccgatcgagg acgtccgagc
     4321 cgccggctat gacggggtct ggcacgggca gaagggcttc aacggagtcg ccattctgac
     4381 gcccgtcgac gccggcccgc ccgagcttcg ccggacgggg ctgcccggcg atcccgacga
     4441 cacccacagc cgctatatcg aagccgaggt cggcggcgtg gtgatcggct cgatctatct
     4501 gcccaacggc aatcccgtcg gaaccgggaa attcgcctac aagctcgcct ggatggagcg
     4561 gctgcgcgag catgctttgg ccctgcttgc ggaggagagg ccggtggtgc tggccggcga
     4621 ctggaacgtc attccgaccg acagccatga cgacgtcttc tcggtccggg cgatggcgca
     4681 cgacgccctg atgcagcccg agagcagggc cgccttccgc cggatcctcc attccggctg
     4741 gacggactcg atccgggccc gccaccccga cggcgccgtc tacaccttct gggactatac
     4801 ggccggctgc tggcagcgcg acgcgggctt ccggatcgac catctcctgc tcagcccgca
     4861 ggccgccgac cggatgctcg atgccggcgt ggacaagagc catcgcggcc gcgagaaggc
     4921 ctcggaccac gccccgacct gggtgcggct cgaagagtga ctgtcactag tgacagtcac
     4981 tagtgacagt caccatcctg gaaggaatgc cgatgcagcc gtggacccgc cgccatttcc
     5041 tcggcaccgc cggtgcggtc gccgccctgc agctgcttcc gggctgcgcc acgcggcgcc
     5101 cggccgggag cggcggatcc gtcgacgcgc tgctttcccg gatggccgag tcgattctcg
     5161 ccgaatatcc ggagaacgcc acgtccctcg gcctggacaa ggacgctcgc gccggactca
     5221 agtctcggct caccgaccgg tcgctccatg ggcgggcgcg gcttgcgggc tccgcggccg
     5281 accggcttcg gcggctccag gcggcggatc gcaccgggcc aggcccggcg gagcagatcc
     5341 atctcggagt cgcggagacg gcgcaccggc tggcggtgga gggattccgc tttccctatg
     5401 gcgacgtcgt caccctcaac cagaattact cctaccggaa cgcgccttat gtggtggccc
     5461 agaataccgg cgcgttcgtc gaggtgcccg acttcctcga cagcagccac agcgtcgcca
     5521 acgccgccga cgcggaggcc tatctcgacc ggctcgaagc ctatgcgggc gctctcgaag
     5581 gagagacgga aaggctggcg cacgaccgcg gcatcggcgt ggtcgcgccc gacttccttc
     5641 tcgacaagac cctgaagcag atgaagggcg cccgctcgca gccgctcggc gagtgggggg
     5701 tggtgacctc gctggagcgg cggacggcgc aggtgccggg cgactggcgg gggagggccc
     5761 ttgccctggc gcgcgacagg gtcgctccgg ccatggaccg gcagatcgcc gagctgaccc
     5821 gccaccgcgc ccgcgccgac agccgggccg gagtgtggaa gctcccggac ggcgaggatt
     5881 attacgcctg ggccctgcgc gccgcgacga ccagcagcct gacgcccgcc gaagtgcaca
     5941 agatgggcct cgaccagctc gccgagctgc agggccggat ggagcccctc ctgcgcgccc
     6001 agggcctgac ccggggcacg gtcggccagc ggatgacggc gctgggcaac gatccgaagc
     6061 acctcttccc gaacacggac gccggccggg cgcagatcct cgaattcgtc aacggcaagg
     6121 tcgcagacat ccgcacccgg ctgccccgcg ccttcgcgac cctcgtcccg ggccggctga
     6181 tcatcaagcg ggttccgcaa gagatcgagg cgggcgctcc gggcggatat gcggcggcgg
     6241 gaacgatcga cggctcggtg cctggcaatt actatatcaa cctgcgcgat accgcgatgt
     6301 ggccgcgctt ctccctcgcg accctgacct atcacgaggg cattcccggc cacatctggc
     6361 agggcgaata tacctacaaa ttgccgctgc tccgctcgct tctcgccttc aacgcctatt
     6421 cggaaggctg ggcgctctac gccgagcagc tcgccgacga gctcggcgcc tacgagggcg
     6481 atccgctcgg cgagctaggc tacctccagt ccctctcctt ccgcgcctgc cgcctcgtcg
     6541 tcgactccgg cctccacgcc cagcgctgga cccgagccag ggccatccag tggttcgccg
     6601 agaccaacgg ctccaccgtc gaggaagtcc agggcgaggt cgaccgctat tgcgcctggc
     6661 cgggccaagc ctgcggctac aaggtgggcc acagcgagat cgtccggctg agggagaagg
     6721 cgagggcggc gctgggcggg aagtttgacc tcaggacgtt cgacgacgcg ctggtgctgg
     6781 gcgggaacgt gcccctgact ttgctcgaag gggtggtgga ccggtacgtt tcgacccgga
     6841 gcggctgatg atcgccctgt cgatcgcagc ggcttttgcc ggactcgcgc cggcggcggc
     6901 tcggcgggct caccctttac gagctgcccg gcgcggggaa ctgacgactc gccggatcgg
     6961 cccgagtgaa tgttcttgat ttgttctcat ttctgtgcga gacaggaacc ctgcccgagt
     7021 cggcccgatc aaccggcgga ggcgggaatc cggctcaagg ggtttgcccc cgaggccggc
     7081 ggccggcgcg aagtcatccg ggaacgtttg agctcgcgtc taccgaggag ccgtgactgc
     7141 gttgtcctcc gaagggttcg gaaaagcggc gtggagcgtt gacggccggc gcccgggctt
     7201 agggcgtgag gagggatgga ggcttcggct tcgatccttt ccaaatcggg gccagcgacc
     7261 ccggacggaa ggaaacgccc gaccctgctt cggatgcccg acggcggcgc cgccctccca
     7321 aggggcggag ccggtgccac ctgagcctcg cggagcgaag gcagcgggac ccggcggctc
     7381 ctcacgataa agtctggacg ctttcgagtt aacgacacga aagcacaaaa agtcgtccag
     7441 ccccgggtgc ccgtggcaag gacgcgccgg ccgcttcttc ccctcatccg gcaacggagg
     7501 cctccgcgcg cgcccgtcat tccggcgaag gccggaaccc atgaacaccg ccgaatgaaa
     7561 cctcgaactt ccctgtccat ggatcccggc ctgcgccggg atgacgaagt cgatccccct
     7621 ccttcagtcg acgtgcctgg ctttcgtgat ggaaggcgca aagccgcgaa ggcgcaacgt
     7681 agaagggaat cgggctgccg ccgagcggcg tcagcggatc gcgctcagct ccaccccctt
     7741 cgtctccttc gccagcaggg cggtgactcc gctgacgaag caggcgacgc ccacatagat
     7801 cgccaccggc aaagcggaat tatattcccg gtagagccac agggcgacga tgggcgccag
     7861 cgagccggcg aggatcgagg tgagctggta ggcgatcgac acgcccgaat agcggatccg
     7921 ggtcgggaag agctcggtga tgaaggcggc ctgggggccg tacatggcac tgtggaggac
     7981 gagcccggcg acgacggcga ggacgatcag ggcgggatcg ccgctggcca gcatcgggaa
     8041 gaaggcgaag ctgaaggcgg cgaggccgag cccgccgaag gcgtagacgg ggcggcggcc
     8101 gatcctgtcg gagaggcggg cgaaaagggg aatcgcgaag aactggacgg cggcgccgat
     8161 cagaagcgcc tgcagcgcct ggctccggct ttccgcggcg acgtcgacga ggaaggtcag
     8221 cgagaagacg gtgaggatgt aataagagat gttctcgccg accct
//
LOCUS       DASWNU010000185         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_214769, whole genome shotgun sequence.
ACCESSION   DASWNU010000185 DASWNU010000000
VERSION     DASWNU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214769"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGB57_03665"
     CDS             complement(<1..186)
                     /locus_tag="VGB57_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130478.1"
                     /translation="MTDETPTDRFNEEKSAYTVRGSDLPDLEAGVAAIRKVVKTLPVR
                     PGVYRMLDAKGDVLYVGK"
     gene            230..550
                     /locus_tag="VGB57_03670"
     CDS             230..550
                     /locus_tag="VGB57_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY0130479.1"
                     /translation="MPTPDDFRRIALSFPGAEEGRHMGHADFRVGGKIFATLGSPDEA
                     WGMAALMPEQQEDFMNLAPDAFKPAAGAWGRGGSTLVRLEAVPEELLETALAAAWRKR
                     APRK"
     gene            complement(855..1346)
                     /locus_tag="VGB57_03675"
     CDS             complement(855..1346)
                     /locus_tag="VGB57_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130480.1"
                     /translation="MLSILAALLASSEPAQPARPQLPGRPAWVVATVAQSEWCPEGDV
                     RLDLRTGEYALTPRAPRRKCEDVRLERPVKTGILDPKRLVALRAVASRAASEGLESQA
                     CRNGERPDKVVVSNGGPQILVLATGATTASAPDDLSCWTEAAEALYHLLDQSFAADSG
                     RSR"
     gene            complement(1514..2104)
                     /locus_tag="VGB57_03680"
     CDS             complement(1514..2104)
                     /locus_tag="VGB57_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY0130481.1"
                     /translation="MAEENPQAPHIREAPGHGAEPAGVVGTPEHHELPTALGLDAPQW
                     IALAMVVVFAILLWKRVPAIIGKALDSKIAAIRAQLDEAAQLRSEAEAIRAEYEARAA
                     QADAEAATLLDRARVEAEGIVRQAESDASALVERRGRMAEDKIAAAERSALDQVRAKA
                     ARAAAAAAESLIREKLDAGADKALVDRTIAGLGQTH"
     gene            complement(2097..2591)
                     /locus_tag="VGB57_03685"
     CDS             complement(2097..2591)
                     /locus_tag="VGB57_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY0130482.1"
                     /translation="MPQINQLADAAYSQFFWLLLVLAILYFGIGRMMLPKIQSTVEAR
                     DQRISGDLAAAQAAREAAEGTEAAYRARMDSTRAEALKVAQAAKVASARATEERMKSA
                     NAEIGAKVEAAEARIRAATDATLGEIESVAAEAAREMVERLAGVSVGSDEAARAVRSA
                     MTHG"
     gene            complement(2635..2862)
                     /locus_tag="VGB57_03690"
     CDS             complement(2635..2862)
                     /locus_tag="VGB57_03690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY0130483.1"
                     /translation="MDFASAQVIGAGLAAIGVGAAAVGVGNVFGSFLESAIRNPAAAD
                     SQQGRLFIGFAAAELLGLIAFAVAMIILYAR"
     gene            complement(2908..>3247)
                     /gene="atpB"
                     /locus_tag="VGB57_03695"
     CDS             complement(2908..>3247)
                     /gene="atpB"
                     /locus_tag="VGB57_03695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0130484.1"
                     /translation="GFHFFSLFIPHGTPKVMLPMIFLIELISFMVRPFSLALRLFVAM
                     IAGHILVEVFGSFVVQGINAGGVGPAVSVLSFIFIVGVMALEILVCAIQAYVFALLTS
                     LYINDAVNLH"
BASE COUNT          534 a         1033 c         1155 g          525 t
ORIGIN      
        1 cttgccgacg tagaggacgt cgcccttggc atcgagcatc cggtagactc cggggcggac
       61 cgggagggtc ttcaccacct tgcggatggc ggcgacgccc gcttccaggt cgggcaaatc
      121 ggacccgcgc acggtgtagg cgctcttttc ctcgttgaag cggtcggtgg gggtctcgtc
      181 ggtcacaggg acgatctaga tgtcggcagc ggtcatgtta aggtgagcca tgccgacgcc
      241 cgacgatttc cgccgcatcg ccctctcctt ccccggcgcg gaggagggcc gccatatggg
      301 ccatgccgac ttcagggttg gcggcaagat cttcgccacg ctcggctccc ctgacgaggc
      361 gtgggggatg gcggcgctga tgccggagca gcaggaggat ttcatgaacc tggcgcccga
      421 cgcgttcaag ccggcggcgg gggcgtgggg gcgcggcggc agcaccctgg ttcggctgga
      481 ggcggtgccg gaggaattgc ttgagacggc ccttgcggcg gcttggcgga agcgggcgcc
      541 tcggaagtag caggtcgagc ctttccgaat cgatcggtgc gaactcattt tagaacgcaa
      601 cgacgcaacg atgccgcgaa gcggacacac gctccgccga tccaacgaag ccgtgcctcc
      661 cgtcggccga ggcggaagcg gagcgctccg cgctcgttgc gtcgtcgcgt tggaaatcaa
      721 ccggcattac gcggagattt ggctcggagg gcgcagctga cggtcacttc ggcgaacgcg
      781 gaagccatca gcactggccg tagctgaccg gagaccagga tgttcatggg tcaggccagg
      841 ggatgacgat ggagctatcg cgaacgcccc gaatcggccg cgaagctctg gtcgagcaga
      901 tgatacaagg cttcggcggc ttcggtccag cagctcaggt cgtcgggcgc ggacgctgtc
      961 gtcgctccgg ttgccaggac gaggatttgc gggccgccat tgctgacgac gaccttatcg
     1021 ggacgctcgc cgttccggca cgcctgtgat tccaggcctt ccgatgccgc gcgcgaggcg
     1081 accgcgcgca gagccacgag ccgcttcggg tcgaggatac ccgtcttcac cggccgttcg
     1141 agcctcacgt cttcgcattt ccggcgcggc gcccgaggcg tgagcgcata ttccccggtt
     1201 cgaaggtcca gcctaacatc tccctcgggg caccattccg actgcgcgac cgtcgccacg
     1261 acccaggccg gtcggccggg aagctgcggc cttgcaggct gggccggttc gcttgaagct
     1321 agaagcgcgg cgaggatgga cagcatgaag cgatcatttc acggccggcg ggcggctgca
     1381 agcgagatgg cggccatgat cgtcattcca gcgaaagccg ggatccatga acacggcgtc
     1441 gcaagcccca caccaccgtg ttcatggatc ccgggtcaag cccgggatga cgttgattgg
     1501 ccgggatgac gggttagtgg gtctggccca gtcccgcaat cgtccgatcc accagcgcct
     1561 tgtccgcccc cgcgtcgagc ttctcgcgga tcagcgactc cgctgcggcg gcggcggccc
     1621 ttgccgcctt ggcccggacc tggtcgaggg cggagcgttc ggcggcggcg atcttgtcct
     1681 cggccatgcg gccgcggcgc tcgacgaggg cggaggcgtc gctttcggcc tggcggacga
     1741 ttccttcggc ctcgacgcgg gcgcggtcga gaagggtcgc tgcctccgca tcggcctgcg
     1801 cggccctggc ttcatattcg gccctgatcg cctcggcctc ggagcggagc tgggcggcct
     1861 cgtcgagctg ggcgcggatg gcggcgatct tcgagtcgag cgccttgccg attatcgcgg
     1921 ggacccgctt ccacaggagg atggcgaaga ccaccaccat cgccagagcg atccactgcg
     1981 gagcgtcgag ccccaaagcg gtcgggagct cgtgatgctc cggggtgccg accaccccgg
     2041 ccggctcggc gccgtggccg ggggcttcgc ggatatgggg ggcctggggg ttttcctcag
     2101 ccatgggtca tcgcactcct gacggctcga gccgcctcgt cgctgcccac cgaaacgccc
     2161 gcaagccgct cgaccatctc gcgcgcggcc tcggcggcga cgctctcgat ctcgccgagg
     2221 gtggcgtcgg tggcggcgcg gatgcgcgcc tcggcggctt ccaccttcgc cccgatctcc
     2281 gcgttcgccg acttcatccg ctcctcggtc gccctggcgc tcgccacctt ggcggcctgg
     2341 gcgaccttca gggcctcggc gcgggtcgaa tccatccggg cgcgataggc ggcctccgtc
     2401 ccttcggcgg cttcacgcgc ggcctgggcc gcggcgagat cgccggagat gcgctggtcg
     2461 cgagcctcga ccgtcgactg gatcttcggg agcatcatcc ggccgatgcc gaaatagagg
     2521 atggcgagca ccagcagcag ccagaagaac tgcgagtaag cggcgtcggc cagctgattg
     2581 atctggggca tgggcgttta gtgacagtca ctagtgactg tcacttcctc cttcttagcg
     2641 ggcgtagagg atgatcatcg cgacggcgaa ggcgatgagg ccgagcagct cggcggcggc
     2701 gaagccgatg aacaggcggc cctgctggct gtcggcggcg gccgggttgc ggatcgcgct
     2761 ctcgaggaac gagccgaaga cgttgccgac gccgaccgcg gcggcgccga cgccgatcgc
     2821 ggcaagaccg gctccgatga cctgggcaga agcgaaatcc atgacaatct ccttgaggaa
     2881 cggggggttt gaaaaacggt gtgaaagcta gtgaaggttg accgcgtcgt tgatgtagag
     2941 cgacgtcaga agggcgaaga cataggcctg gatggcgcag accaggatct cgagcgccat
     3001 cactccgacg atgaagatga aggagaggac gctgacggcg gggccgactc cgccggcgtt
     3061 gatcccctgg acgacgaagc tcccgaacac ctcgacgagg atgtgccccg cgatcatcgc
     3121 gacgaaaagc cggagcgcga ggctgaacgg gcggaccatg aaggagatca attcgatgag
     3181 gaagatcatc ggaagcatca ccttgggcgt gccgtgcggg atgaacagcg agaagaagtg
     3241 gaagccg
//
LOCUS       DASWNU010000186         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_474939, whole genome shotgun sequence.
ACCESSION   DASWNU010000186 DASWNU010000000
VERSION     DASWNU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474939"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGB57_03700"
     CDS             complement(<1..572)
                     /locus_tag="VGB57_03700"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130485.1"
                     /translation="MFSRSLLALLALGAALPATLAPASAQSAAELTGPQAAPAPPPAA
                     VATVIGADWSRKDAVKLLRYVEGIGSEGLDPADYNVSALRAAINSEDEGLSRIATATF
                     LKVASDLKFGHVRDSGRVDWHVKDDSWNVYDQQALLDRAAKGDVAETFESLLPTHPQY
                     AALKGLLADKSLDSKTRDRVRINLDRWRWLP"
     gene            complement(834..1253)
                     /locus_tag="VGB57_03705"
     CDS             complement(834..1253)
                     /locus_tag="VGB57_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY0130486.1"
                     /translation="MNTRTFALIFGIVFLVIGAGGFIPGLTDHSAAPDPGLTMRHGFG
                     HELGLFPINTLHNIVHLIFGIWGIMAYKSYSGSRSYFRVVAVVYGLLTILGLLEPTRT
                     TFGLVPIYGNDVWLHALLALVAAYFGWVNRDTAGDRV"
     gene            complement(1281..1928)
                     /locus_tag="VGB57_03710"
     CDS             complement(1281..1928)
                     /locus_tag="VGB57_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY0130487.1"
                     /translation="MSHVVLLGDSILDNAAYVRGGPDVVRQLRGKLPADWNASLLARD
                     GAVTREVRSQLARLPKDATHLVVSAGGNDALGASFLLGRRVGTVAEAVSLLENAQSHF
                     AADYEEMAEAVAATGLPAAFCTVYDTPSSSPDHRIVRTALAVFNDVITRAAFSRGAGL
                     IDLRLICDEDEDYANPIEPSVQGGDKIASAIAAWAAAGNPTGYGAVLTLRDTEYA"
     gene            complement(1946..>2838)
                     /locus_tag="VGB57_03715"
     CDS             complement(1946..>2838)
                     /locus_tag="VGB57_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130488.1"
                     /translation="GPGHQLQHAMTGRIAILTPDRNSEGFRTRWPDVLDRNASPLREA
                     GLEVESRDWADSTDLAGFDLVLPLLVWGYPFAPEQWRASVDRWEGEGVRLRNPPSVLR
                     WNADKIYLERLAERGAPVVPTRHCTRLTEAALEEAARAFGTDRLVAKPRVSSSAWQTI
                     RWSPGAPLAGGPEGAAMVQPYLPEIERSGEVSMIFFGGAFSHAISKRPQPGDFRVQPE
                     YDGIIAAHRPDAAEHEAAERILAAVDEPLLYARVDLVRGLDGAPQLIELELIEPDLYL
                     GYEEDAGKRFAAAILELIAG"
BASE COUNT          479 a          932 c          998 g          429 t
ORIGIN      
        1 ggaagccagc gccagcggtc gaggttgatc cggacccggt cgcgggtctt ggagtcgagc
       61 gacttgtcgg cgagcagccc cttcaaggcg gcatattgcg ggtgggtcgg gagcagcgac
      121 tcgaaggtct cggcgacgtc gcccttggcc gcccggtcga gcagggcctg ctggtcgtag
      181 acgttccagc tgtcgtcctt gacgtgccag tcgacccggc ccgaatcgcg gacgtggccg
      241 aacttgaggt cggaagcgac cttgaggaat gtcgccgtcg cgatccggct gaggccttca
      301 tcttcggagt tgatggcggc acgaagcgcc gagacattgt agtcggccgg gtcgagccct
      361 tccgagccga tgccctcgac gtagcgcaga agcttcaccg cgtccttgcg cgaccaatcg
      421 gcgccgatga cggtagcgac ggcggcgggc ggcggagccg gagcggcttg gggccctgtc
      481 agctcggcgg cactctgggc ggaagcgggc gccagcgttg cgggcaaggc ggctccaagc
      541 gcgaggagag cgagtaaact gcggctgaac atggttaatc cccgttaatg gcggccgcga
      601 aaaagctccg cgtggcagtg cgccattatc ctgtgactac ctattaaacc ccgctgaacg
      661 gacccgcaag gcccgggcaa gccttggaaa ggaaaaggga aacggcccgg cgcaaggccg
      721 ggccgttttg tccctgcccc gtcgttgcga ggagcgcagc gacgaagcaa tccagtttcc
      781 gtaagtctga gccgctggat cgcttcgcta cgctcgcaat gacggcggcg ccgtcagacg
      841 cggtcgcctg cggtgtcgcg attgacccag ccgaaatagg ccgcgaccag cgcgagcagg
      901 gcgtgcagcc agacgtcgtt gccgtagatc gggacgaggc cgaaggtcgt ccgcgtcggc
      961 tcgagcagcc cgaggatggt caggagcccg tagaccaccg cgacgacccg gaaataggag
     1021 cgggagccgc tgtagctctt gtaggccatg atcccccaga tgccgaagat caggtggacg
     1081 atgttgtgga gggtgttgat cgggaacagc ccgagctcgt gcccgaagcc gtgccgcatg
     1141 gtgaggcccg ggtccggcgc ggcggaatgg tcggtcaagc cgggtatgaa cccgccggcg
     1201 ccgatgacga gaaagacgat tccgaaaatg agggcgaagg tccgcgtatt catgatccaa
     1261 ttcccctggg tttcttgtgt ttacgcgtac tcggtgtctc ttagcgtaag aacggctccg
     1321 taaccagtcg ggttcccggc cgccgcccag gcggcgatcg ccgacgcgat cttgtccccg
     1381 ccctggacgg acggctcgat cggattggcg taatcctcgt cctcgtcgca gatcaggcgc
     1441 aggtcgatga gcccggcgcc gcgcgagaag gcggcgcggg tgatgacgtc gttgaagacg
     1501 gcgagggccg tccggacgat gcgatggtcc ggcgacgagg agggcgtgtc gtagaccgtg
     1561 cagaaagcgg cgggcaggcc ggtcgcggcg accgcctccg ccatctcctc ataatcggcg
     1621 gcgaagtggc tctgcgcatt ctcgagcagc gacaccgcct ccgcgaccgt gccgacccgc
     1681 cggccgagca ggaaggaggc accgagcgcg tcgttgccgc cggcgctgac gacgaggtgg
     1741 gtggcgtcct tcggaagccg ggccagctgg gaacggacct cgcgtgtcac cgcgccgtcg
     1801 cgcgcgagca ggctcgcatt ccaatcggcg ggcagcttgc cgcgcagctg gcgcacgacg
     1861 tccgggccgc cccgcacgta agcggcgttg tcgaggatcg aatcgccgag gagaacgaca
     1921 tgggacatgg tcccgcctca gcggctcaac cggcgatcag ttccagaatt gccgccgcga
     1981 aacgttttcc cgcatcctct tcataaccca ggtaaagatc gggctcgata agctccagct
     2041 cgatcagctg cggcgccccg tcgaggccgc gaaccaggtc gacccgcgcg tagaggagcg
     2101 gctcgtcgac cgcggccagg atccgctccg ccgcctcgtg ctccgccgcg tcgggccggt
     2161 gagcggcgat gatcccgtca tactccggct gcacccggaa gtcgccgggc tgaggccgct
     2221 tggagatggc atggctgaac gctccgccga agaagatcat cgacacctct cccgagcgct
     2281 cgatctccgg cagatagggc tggaccatcg cggcgccctc cggcccgccc gccagaggag
     2341 cgcccggcga ccacctgatc gtctgccagg cgctcgagga gacgcgcggc ttggcgacca
     2401 gccggtccgt gccgaacgcc cgcgccgcct cctccagagc ggcctcggtc aaccgggtgc
     2461 aatggcgggt ggggacgacc ggcgcgccgc gttccgcgag ccgctccaga tagattttgt
     2521 ccgcgttcca gcgcagcacc gacggcggat tgcgcagccg cacgccctcc ccttcccagc
     2581 ggtcgaccga ggcgcgccac tgctcgggag cgaaaggata gccccagacc agcagcggca
     2641 gcaccaggtc gaacccggcc agatcggtcg agtcggccca gtcgcggctc tcgacctcaa
     2701 gccccgcctc gcgcagcggc gaggcgttgc gatcgagcac gtccggccag cgggtgcgga
     2761 agccctcgct gttcctgtcc ggggtgagga tcgcgatccg gcccgtcatg gcatgttgaa
     2821 gttgatgccc tgggccag
//
LOCUS       DASWNU010000187         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_447967, whole genome shotgun sequence.
ACCESSION   DASWNU010000187 DASWNU010000000
VERSION     DASWNU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447967"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..249)
                     /locus_tag="VGB57_03720"
     CDS             complement(58..249)
                     /locus_tag="VGB57_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130489.1"
                     /translation="MKNRLKVLRAERDWSQSDLAERLEVSRQSVNAIETGRYDPSLPL
                     AFRIANLFALPVEAIFEAE"
     gene            complement(246..641)
                     /locus_tag="VGB57_03725"
     CDS             complement(246..641)
                     /locus_tag="VGB57_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130490.1"
                     /translation="MSFRSPARSRYNHRVLGLSLAYAGALIGVSFLFRSSPPTGPLAW
                     VLAILPALPLVGIFIAMGRYLVEEGDEYLRAVESRKALIATGFMLIVTTCWGFLQSFD
                     QVPRVDFYWAAIVWFFGLGLGGCVGRFAR"
     gene            complement(698..1441)
                     /locus_tag="VGB57_03730"
     CDS             complement(698..1441)
                     /locus_tag="VGB57_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0130491.1"
                     /translation="MGSPDYFDAGPGRRLAFRHRRGSGPALVFLPGYMSDMEGSKASA
                     LDSWAQAQGRAMLRFDYAGCGLSEGEFEAQTLAAWRDDALALIDRVEGPLVLIGSSMG
                     GWLMLLCALARPKRIRGLVGIAAAPDFTDWGFDEAQKRTIRDDGRLEEPSPHGDAPYV
                     TTRAFWESGEALKLLGGDIAISCPVRLLHGLDDRDVPWRTSLRLTERLASPDVRTILV
                     KGGDHRLSRPGDIALLIDTVERLPPDKDP"
BASE COUNT          295 a          522 c          586 g          246 t
ORIGIN      
        1 aaggccggga cccagccctt ccgtcggcgc cgctgggccc cggccttcgc cggggaacta
       61 ttccgcctcg aagatcgcct cgaccggcag tgcgaacaag ttggcgatcc ggaaagccag
      121 tggaaggctc ggatcgtagc ggccggtctc gatcgcgttg acgctctggc gcgagacttc
      181 gagccgctcg gcgaggtcgc tttggctcca gtcgcgctcg gcccggagga ccttgaggcg
      241 gttcttcatc tcgcgaaccg gccgacgcag ccgcccaggc cgaggccgaa gaaccagacg
      301 atggcggccc agtagaaatc gactcgcgga acctggtcga acgactggag gaagccccag
      361 catgtcgtca cgatcagcat gaagccggtg gcgatcagcg ccttccggct ctccaccgcg
      421 cgcagatatt cgtcgccttc ctcgaccaga taacggccca tggcgatgaa gattccgacc
      481 agaggcagag cggggagaat tgcgagaacc caggccagcg ggcccgtggg cggcgaggag
      541 cggaacagga agctgacccc gatcagagcg cccgcataag cgaggctgag gccgagcact
      601 cgatgattgt agcggcttcg ggcggggctt cggaacgaca tgtcaaggct ccttgagttt
      661 cagtcaagga agctttacag aatgattgtc gggcttgtca agggtccttg tcaggcggaa
      721 gacgttccac cgtgtcgatc agaagggcga tgtcgccggg tctggaaagg cggtggtcgc
      781 cgcccttgac gaggatcgtc cgcacgtcgg gcgaggcgag ccgctcggtc agacggagcg
      841 aggtgcgcca gggcacgtcg cgatcgtcga ggccgtggag caggcggacg gggcaggaga
      901 tggcgatgtc gccgcccagc agcttcagcg cttcgccgct ttcccagaag gcgcgggtgg
      961 tgacataggg cgcatcgcca tgaggcgagg gctcctcgag gcggccgtcg tcccggatcg
     1021 tccgcttctg cgcctcgtcg aagccccagt cggtgaagtc cggagcggcg gcgattccga
     1081 ccaacccccg gatccgcttg ggccgggcga gggcgcagag cagcatcagc cagccgccca
     1141 tagaggagcc gatgaggacg agcggcccct cgacccggtc gatcagggcg agcgcgtcgt
     1201 cgcgccaggc ggcaagggtc tgggcttcga actcgccttc gctcaggccg cagccggcat
     1261 aatcgaagcg gagcatcgct cggccctgcg cctgcgccca ggagtcgagc gcgctcgcct
     1321 tgctcccttc catgtcggac atgtagccgg gcaggaagac gagggccggg ccgctgccgc
     1381 ggcgatgacg gaaggcgagg cggcggccgg ggccggcgtc gaaatagtcg ggggagccca
     1441 ttcggcggtc tctaaagcag agccgactaa agctgtcacg cttccctccc cccgccgtca
     1501 ttccggcgaa cgccggaatc catgaacgcg gagggccgag gcttcgtacg cccgtgttca
     1561 tggatcccgg gtcaagcccg ggatgacgct gaacggtcga taaatgctcg ggggctggag
     1621 atggagcgag cctgtcgcta catgccccc
//
LOCUS       DASWNU010000188         5378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_344123, whole genome shotgun sequence.
ACCESSION   DASWNU010000188 DASWNU010000000
VERSION     DASWNU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344123"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VGB57_03735"
     CDS             complement(<1..889)
                     /locus_tag="VGB57_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0130492.1"
                     /translation="MAITPLMPVYPRSPVRPVRGEGVYLYGEGGEKYLDFASGIAVNL
                     LGHGHPHLTKAIQEQAATLIHVSNLYGSPQGEALAQRLVDNSFADTVFFTNSGAEAVE
                     CAIKTARRYHHHHGRPEKHNLITFRNAFHGRTMATISATDQAKLRDGFAPMLPGFTVV
                     DFDDLEAAEAAVDENTAGFLVEPVQGEGGIRPASPEFLRGLRRICDEKDLMLVLDEVQ
                     CGVARTGRLYAYEHYGIEPDILASAKGIGGGFPMGACLATEKAAAGMVIGTHGSTYGG
                     NPLAMAAGQAVLDVVTEPEF"
     gene            complement(1007..1393)
                     /locus_tag="VGB57_03740"
     CDS             complement(1007..1393)
                     /locus_tag="VGB57_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130493.1"
                     /translation="MRKFLIPALVLSTVVGVAPAAAQHGGYHNPNRGNVAWNRGGPSR
                     QAINELLRDLQRAENGIQRSVQRRAISQREANSLAREANQIERRLNLASRNGISGREF
                     GALRVQVNRLQQRLRIERQDRDGRRY"
     gene            complement(1533..2348)
                     /locus_tag="VGB57_03745"
     CDS             complement(1533..2348)
                     /locus_tag="VGB57_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0130494.1"
                     /translation="MRAAPGVPVIRNVNWGGLRTLYVKEVRRFFMVQLQTVWGPALTT
                     LLFLVIFSVALGRSDRMVQLGASSFHFADFLAPGLIVMGMLQNAFANSSFSLLVGKIQ
                     GNIVDYLMPPLSTAELITALIGAAVTRAAMVGLAVFLAMQLWPGVHVAPVHFWAVLWF
                     GLMGSMMLAFFGLITSIWAEKFDHAAAVTNFVVAPLSLLSGTFYSIDSLAPAFRAISH
                     ANPFFYVISGFRYGFLGAADSPILLGALLLLGINLALGIATYAMLRTGWKLKA"
     gene            2447..3124
                     /locus_tag="VGB57_03750"
     CDS             2447..3124
                     /locus_tag="VGB57_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEY0130495.1"
                     /translation="MSWTDERIDRLKELWTNGMTASQIADELGGVSRNAVIGKAHRLG
                     LQSRPSPVKPNEGGEGNAAPSPFEPEAPVAAEAAAEPEDIQAEAPETGIEAEAQPASA
                     PAEPAAAPAPPQPQIRSIGPGGFVRQAPGEQQSPIPPAPPRRLVPAKPSPEIADKTSL
                     LDLNEKICKRPIGHPGEPDFHFCGAQANPGFPYCVEHCGVAYQAQIPRRDRRPPPPLP
                     FGGPRIR"
     gene            3221..3391
                     /locus_tag="VGB57_03755"
     CDS             3221..3391
                     /locus_tag="VGB57_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130496.1"
                     /translation="MSQEEQEYFERRAEAELELAQAAAHRRAVQAHYELASAYLDRIH
                     GDEPRSEGGLFA"
     gene            3412..4773
                     /locus_tag="VGB57_03760"
     CDS             3412..4773
                     /locus_tag="VGB57_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY0130497.1"
                     /translation="MAFLPKLLLAALVLPLSALAFQRSAPDSGRAPPPAQSIAAVRES
                     ARPIAGTGADYADILAATAGARRILLGESTHGTHEYYRERGRLTERLIREQGVNAVAI
                     EGDWSATQRVNLYVRGLGSDRSAAQALRGFTNFPTWMWPNADFRDFVERLRAFNLERP
                     AGQRVGLYGMDVYDLFDSAEAVQAWLKGAAPEAGRRAAGHYRCFARYGRDRWAYGTAT
                     RDPRRSCRAQAQAVLADVRRLPRPADPIAAEARFAALRSAASVAGAEEYFRTASVGSL
                     AWNVRDRHMADNVDEIADHVQAMTGRPGKVVMWSHNTHSGDARATFAADRGELNLGQL
                     MRQRHGQGTFLIGFFSHSGTVFAAPEWDSKGRRYDMRPALAGSYSALFHEARLPAFSL
                     LIRGNPAVSRLLAGPMLERAIGVIYLPNSERTAHYFDARLAEQFDAAVYFDRTTAVTP
                     LGR"
     gene            4770..>5378
                     /locus_tag="VGB57_03765"
     CDS             4770..>5378
                     /locus_tag="VGB57_03765"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0130498.1"
                     /translation="MTRKGLLLALPLLLAAAPAQDRAAERELMVLTVANMAETAGLQS
                     RRIDPAVLRAMREVPRHLFVPEEARPFAYENRPLPIGHEQTISQPYIVALMTDLLKAR
                     PGDVVLEVGTGSGYQAAILSRLGARVHSIEIVEPLATRAKARLAELGYADVEVRGGDG
                     YAGWPERAPFDRIIVTAGASHVPQPLIDQLRTGGKIVIPVGKA"
BASE COUNT          880 a         1858 c         1821 g          819 t
ORIGIN      
        1 gaaattcggg ttcggtgacg acgtcgagca ccgcctggcc ggcggccatg gcgagcgggt
       61 tgccgccgta ggtggaaccg tgcgtcccga tcaccatgcc ggcggccgcc ttctcggtgg
      121 cgaggcaggc acccatcggg aagccgccgc caatgccctt ggccgaggcg agaatgtcgg
      181 gctcgatccc gtaatgctcg taggcgtaga gcctgccggt gcgggcgacc ccgcactgga
      241 cctcgtcgag gacgagcatg aggtccttct cgtcgcagat ccgcctcagg ccgcggagga
      301 attccgggct cgccggacgg atgccgcctt cgccctggac cggctcgacc aggaagccgg
      361 cggtgttctc gtcgacggcg gcttccgcgg cctccagatc gtcgaagtcg acgacggtga
      421 agcccggcag catcggcgcg aagccgtccc gaagcttcgc ctggtcggtc gcgctgatcg
      481 tcgccatcgt ccggccgtgg aaggcgtttc gaaaggtgat cagattgtgc ttctcgggcc
      541 ggccgtggtg atggtggtag cggcgcgccg tcttgatcgc gcattcgacc gcctcggcgc
      601 ccgaattggt gaagaagacc gtatcggcga agctattgtc gaccagccgc tgcgcgagcg
      661 cctcgccttg aggcgacccg tagagattgg agacgtggat cagggtcgcc gcctgctcct
      721 ggatcgcctt cgtcagatgg gggtggccgt ggccgagcag gttgacggcg atgccgctgg
      781 cgaaatccag atatttctcg ccgccctcgc catagagata gaccccctcc cctcgcaccg
      841 gccgcacggg cgagcgcggg tagacgggca tgagcggcgt gatggccatg aggggctccg
      901 ggctgtgaat gcaaaaaggc gacccttaac ggatcgcctt cctgctctct agagagagga
      961 cgggcgccgc gtcaattcgc ggcgcccgcc cgttgcgctt tcgcttttag tagcggcggc
     1021 cgtcgcggtc ctggcgctcg atgcgcaggc gctgctggag ccggttgacc tggaccctca
     1081 gcgctccgaa ctcgcggccg ctgatgccgt tgcggctggc caggttgagc cggcgctcga
     1141 tctggttggc ctcgcgggcc aggctgttcg cctcgcgctg cgagatggcc cggcgctgga
     1201 ccgaacgctg gatgccgttc tcggcccgct ggaggtcgcg aagcagctcg ttgatcgcct
     1261 ggcgggacgg gccgccacgg ttccaggcga cgttgccgcg attgggattg tgatagccgc
     1321 cgtgctgagc cgcggccgga gcgacgccga cgacggtgga aagcaccagg gcggggatga
     1381 gaaacttacg catgttactt gctccttggt tggtagggag cttctacgac tcggcctctg
     1441 atgcggcgct gaacggggtc gacagcgccc gttcatctaa tcgggcattt ttatgaacgc
     1501 cttgcctccc atcgccggat ggggaggcaa ggttacgcct tcagcttcca gcccgtgcgc
     1561 aacatggcgt aagtcgcgat gccgagcgcc aggttgatgc cgagcagcag cagggcgcca
     1621 agcaggatcg gcgaatcggc ggcgccgagg aagccgtagc ggaagcccga aatgacgtag
     1681 aagaagggat tggcgtggct gatcgcgcgg aaggccgggg cgaggctgtc gatcgagtag
     1741 aaggtgcccg acagcaggga gagcggcgcg accacgaaat tggtgaccgc ggcggcatgg
     1801 tcgaacttct cggcccagat cgaggtgatc agcccgaaga acgccagcat catgcttccc
     1861 atcagaccga accagagcac cgcccagaaa tggaccggcg cgacatggac tcccggccac
     1921 agctgcatgg cgaggaacac ggcgaggccg accatggcgg cccgggtgac ggcggcgccg
     1981 atcagggcgg tgatcagctc ggccgtcgac aaaggcggca tcagataatc gacgatattg
     2041 ccctggatct tgccgaccag cagcgagaag ctcgaattgg cgaaggcgtt ctgaagcatg
     2101 cccatcacga tgaggccggg cgcgaggaaa tcggcgaagt ggaagctcga cgcgcccagc
     2161 tggaccatcc ggtcgcttcg ccccagggcc acgctgaaaa tgaccaggaa cagcaatgtg
     2221 gtcagggccg gaccccacac cgtctgcagc tggaccatga agaaacgcct cacctccttc
     2281 acatagaggg tcctgaggcc gccccagttc acgttccgga tcaccgggac gcccggggcg
     2341 gcccgcatcc aattcgaagg ctggtcgttc accgcgccgt cgctagtggc agacgccgtg
     2401 agccgcaagc acagagccgc aagcatataa ggaaggcgcc gcaataatgt cctggaccga
     2461 cgaacggatc gaccggctga aggagctctg gaccaacggc atgacggcga gccagatcgc
     2521 cgacgagctc ggcggagttt cccgcaacgc tgtgatcggc aaggcccatc gcctcggcct
     2581 ccagtcgcgc ccgtcgccgg tgaagccgaa cgagggcggc gagggcaatg ccgcgccgtc
     2641 gccgttcgag cccgaagctc cggtggccgc cgaagccgcg gccgagccgg aagatatcca
     2701 ggcggaagct ccggaaaccg gaatcgaagc ggaagcgcag ccggcctcgg ctccggccga
     2761 gcccgcggcg gcccccgcgc ccccgcagcc ccagatccgc tcgatcggcc ccggcgggtt
     2821 cgtccgccag gctccgggag agcagcagtc gcccatcccg ccggccccgc cgcgccgcct
     2881 cgtcccggcc aagccgagcc cggagatcgc cgacaagacc agccttctcg atctcaacga
     2941 gaagatctgc aaacggccga tcggccaccc cggcgaaccc gacttccact tctgcggagc
     3001 tcaggccaat cccggcttcc cttattgcgt cgagcattgc ggggtcgcct atcaggcgca
     3061 gatcccgcgc cgcgaccgcc ggccgccgcc gccgcttccg ttcggcggcc cccgcattcg
     3121 ctgaggatca acaacctgga ttagcgtgat tgcgcggaac ggcggcagcg gtttggccgt
     3181 tttgataccc gtccgtaacc aacgtaaaaa tccaggttaa atgtctcagg aagaacagga
     3241 atatttcgaa cgtcgggcgg aggcggagct tgagctcgcc caggccgcgg cgcatcgccg
     3301 cgccgtccag gcgcattacg agctcgcttc cgcctatctc gaccggatcc acggtgacga
     3361 gcccagatcc gagggcgggc tcttcgcctg atcggcgagc gaggcttcgt catggccttc
     3421 ctgccgaagc ttctcctcgc cgccctggtc ctcccgctca gcgcactggc cttccagcga
     3481 agcgccccgg actccggccg cgcgcctccg cccgcccagt cgatcgcggc ggtgagggag
     3541 tcggcgcggc cgatcgccgg gaccggggcg gattatgccg acatactcgc cgcgacggcc
     3601 ggcgctcgcc ggatcctgct cggcgaatcc acccacggca cccacgaata ttatcgcgag
     3661 cgaggccggc tgaccgagcg gctgatccgc gagcaggggg tgaacgcggt cgcgatcgag
     3721 ggggactggt cggcgaccca gcgagtcaac ctctacgtgc gcgggctcgg ctcggaccgg
     3781 agcgccgcgc aggcgctgcg cgggttcacc aacttcccca cctggatgtg gcccaacgcc
     3841 gatttcaggg atttcgtcga gcggctccgg gcgttcaacc tcgaacgccc ggccgggcag
     3901 cgcgtcggcc tctacggaat ggacgtctac gacctgttcg actccgccga ggcggttcag
     3961 gcctggctga agggggcggc tccggaagcg ggccggcgcg ccgccggtca ttaccgctgc
     4021 ttcgcccgct acgggcgcga ccgctgggcc tatggcaccg ccacgcgcga tccccgccga
     4081 tcgtgccggg cccaggccca ggcggttctc gccgacgtcc gccgactgcc gaggccggcc
     4141 gatcccattg ccgccgaggc gcgcttcgcc gcgcttcgaa gcgccgcctc ggtcgctggg
     4201 gcggaggaat atttccggac cgcctcggtc gggtcgctcg cctggaacgt ccgcgaccgc
     4261 cacatggccg acaacgtcga cgagatcgcc gaccatgttc aggccatgac cggccgcccc
     4321 ggcaaggtgg tgatgtggag ccacaacacc cattcgggag acgccagggc gaccttcgcc
     4381 gcggatcggg gcgagctcaa cctcggccag ctgatgcgcc agcggcacgg ccaggggacg
     4441 ttcctgatcg gcttcttcag ccattcgggc accgtcttcg cagcgcccga atgggattcg
     4501 aaaggccgcc gctacgacat gcggccggcg ctggccggaa gctattccgc gctcttccat
     4561 gaagcccggc ttccggcctt ctcgctgctc atccggggca atccggccgt ctcgcggctc
     4621 ctcgccgggc cgatgctcga gcgggcgatc ggcgtgatct acctgccgaa cagcgagcgc
     4681 acggcccatt atttcgacgc tcgcctggcc gagcagttcg acgccgcggt ctatttcgac
     4741 cgcaccaccg ccgtcacccc gctcggccgg tgacccgcaa gggcctgctc ctcgctttgc
     4801 cgctcctcct cgcggccgcg ccggcgcagg accgcgccgc cgagcgcgaa ctgatggtcc
     4861 tgaccgtcgc caacatggcg gaaaccgcag ggctccagtc gcgccggatc gatcccgcgg
     4921 tgctccgggc gatgcgcgag gtgccccgcc acctgttcgt cccggaggag gcgaggccct
     4981 tcgcctatga gaaccggccg ctcccgatcg gccatgagca gaccatctcc cagccctata
     5041 tcgtcgccct gatgaccgac ctgctcaagg cccggccggg cgacgtcgtg ctcgaggtcg
     5101 gcaccggctc cggctaccag gcggcgatcc tgtcgcggct cggcgcccgg gtccattcga
     5161 tcgagatcgt cgagccgctc gcgacccggg ccaaagcgcg cctggccgag ctcggctatg
     5221 ccgatgtcga ggtgcgcggc ggcgacggct atgccggctg gcccgagcga gcgcccttcg
     5281 accggatcat cgtcaccgcc ggcgccagcc acgtgccgca gccgctgatc gatcagctta
     5341 ggaccggcgg caagatagta atccccgtcg gtaaggca
//
LOCUS       DASWNU010000189         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_111448, whole genome shotgun sequence.
ACCESSION   DASWNU010000189 DASWNU010000000
VERSION     DASWNU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111448"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGB57_03770"
     CDS             <1..813
                     /locus_tag="VGB57_03770"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY0130499.1"
                     /translation="VTTRSSPPSRQLTHGTTLHGVQNLTPGGETEPTSYYARRSGVGH
                     AIDSLPIFHGPNASIGVVGLGTGTLSCYARPGQDWRFFEIDPAMVRIARNPEIFTFLS
                     RCAPQARVILGDARLSLAREPAGKLDLLAVDAFSSDAIPIHLLTREALQVYRRALKPD
                     GLLLIHISNRYLDLEPVLAAAAKRDGWHAAAFDYSPEAGEEGRNLSRSIWVAMTRDKD
                     GLMTLRIASAEDAHLWRPLLGRPGFEGWTDDYASILPLLEDWNAWLPGVLKP"
     gene            813..1349
                     /locus_tag="VGB57_03775"
     CDS             813..1349
                     /locus_tag="VGB57_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0130500.1"
                     /translation="MIETERLWLRDWREEDIEPFIRHLNVEPVMRWLAGVRTPEQQEA
                     AVRERFMLWQQTLGFTFWVVERKEDGELLGFCGLKIADDPQSPVEGEYEVGWRLREDA
                     WGKGYAKEAAIASLYFAFDRLGAERVVALTVDGNAPSWGLMIRLGMTRRADLDYDTAQ
                     WAEGPVIVYEMRRQQWRS"
     gene            1337..>1598
                     /locus_tag="VGB57_03780"
     CDS             1337..>1598
                     /locus_tag="VGB57_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0130501.1"
                     /translation="MAKLIPFGGKAPLVDPTAFLAPGAVLIGDIEIGAEASIWYNCVL
                     RGDVNHIRIGARTNVQDGTVLHVDSPRPGSPAGLPTIIGEDVL"
BASE COUNT          254 a          553 c          533 g          258 t
ORIGIN      
        1 gtcactaccc ggagcagccc gccctcgcgc cagctcaccc acggcaccac cctccacgga
       61 gtccagaacc tcactcccgg gggcgagacg gagccgacct cttattacgc tcgccgctcc
      121 ggagtgggcc atgcgatcga ctcgctgccc atctttcacg gccccaacgc ttcgatcggc
      181 gtggtcggcc tcggcactgg aaccttgtcc tgctacgccc ggccggggca ggactggcgc
      241 ttcttcgaga tcgatccggc gatggtccgg atcgccagga acccggagat cttcaccttc
      301 ctctcccgct gcgcgccgca ggccagggtg atcctcggcg acgcccggct cagccttgcc
      361 cgcgagcccg cgggcaagct cgacctgctc gccgtcgacg ccttttcgtc cgacgccatc
      421 cccattcacc tcctcacccg cgaggcgctg caggtctacc ggcgggcgct caagcctgac
      481 gggctgctcc tcatccatat ctccaaccgc tatctcgatc tcgagccggt gctcgccgcg
      541 gcggcgaagc gcgacggctg gcacgccgcc gctttcgatt attcgccgga ggcgggcgag
      601 gaggggcgca atctgtcgcg ctcgatatgg gtggcgatga cgagggacaa ggacggtttg
      661 atgaccctca ggatcgcaag cgccgaggac gcccatctct ggcggccgct gctcggccgg
      721 cccggcttcg agggctggac cgacgattat gcaagcatcc tgccgctgct cgaggattgg
      781 aacgcctggc tgcccggagt gctgaagcca tgatgatcga gaccgagcga ctttggctgc
      841 gcgactggcg cgaggaggat atcgagccct tcatccgcca cctcaacgtc gagccggtga
      901 tgcgctggct cgccggcgtc cgaacgcccg agcagcagga ggcggcggtc cgcgagcgct
      961 tcatgctgtg gcagcagacg ctcggcttca ccttctgggt ggtcgagcgc aaggaggatg
     1021 gcgagctgct cggcttttgc ggcctcaaga tcgccgacga tccgcaaagc ccggtcgagg
     1081 gcgagtacga ggtcggctgg cggctgcgcg aggacgcgtg gggcaagggc tatgcgaagg
     1141 aagcggcgat cgcctcgctc tatttcgcct tcgatcggct cggcgccgag cgcgtcgtcg
     1201 ccctgaccgt cgacggcaac gcgccgagct gggggctgat gatccggctc ggaatgacac
     1261 ggcgggccga cctcgattac gacaccgccc agtgggccga gggcccggtc atcgtctacg
     1321 agatgaggcg ccagcaatgg cgaagctgat ccccttcggc ggcaaggcgc cgctcgtcga
     1381 tcccacagcc ttcctcgccc cgggggcggt cctgatcgga gacatcgaaa tcggcgcgga
     1441 agccagcatc tggtacaatt gcgtcctgag gggcgacgtc aaccatatcc ggatcggcgc
     1501 ccggaccaac gttcaggacg ggaccgtcct gcacgtcgat tcgccgaggc cgggaagccc
     1561 ggcgggcttg ccgacgatca tcggcgagga cgtgctga
//
LOCUS       DASWNU010000190         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_317420, whole genome shotgun sequence.
ACCESSION   DASWNU010000190 DASWNU010000000
VERSION     DASWNU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317420"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGB57_03785"
     CDS             <1..358
                     /locus_tag="VGB57_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0130502.1"
                     /translation="ATWARVRRRSPPPAQSNRIAEKPSLLARARIDYAHASGVSTRLE
                     AEHVGRAYSTDPDGALVPLKRSTSFNWRLAYALPFRNKGFELFLHVDNVADTFIEPQL
                     GLPAPGRSFRVGLRLD"
     gene            496..969
                     /locus_tag="VGB57_03790"
     CDS             496..969
                     /locus_tag="VGB57_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130503.1"
                     /translation="MHNDTSGPAPASSDVHRPDDVPGFLRFAPVPVRARRDGWTAERQ
                     LRFILALARGARAGEAARRLGRSRQSAYALRRRAGAEAFAAAWDGAVDFAAEARGAPG
                     MPGASSSGIETLLVPRYYRGRLIGFVQREDLRGLMARLSRLDRMAERLEARAAGR"
     gene            complement(1054..1455)
                     /locus_tag="VGB57_03795"
     CDS             complement(1054..1455)
                     /locus_tag="VGB57_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY0130504.1"
                     /translation="MAYWLMKSEPGTYSWDDLVRDGRTDWDGVRNPAARLHLRAMKAG
                     DEAFFYHSGDERAVVGIMRLAGEGSPDGADGAWVKVPVEPVRPLGPVTLKAIKAEPRL
                     AKMELIRQSRLSVSPVREEEWAAVLEMAKAG"
BASE COUNT          212 a          534 c          523 g          289 t
ORIGIN      
        1 tgcgacatgg gcccgcgtcc gtcggcgaag cccgcctccg gcacaatcga acaggattgc
       61 tgaaaagccg tcgctgctcg cccgcgcccg catcgattat gcccacgcct cgggagtttc
      121 gacgcgcctc gaggcggaac atgtgggccg cgcctattcc accgacccgg atggtgcgct
      181 ggtgccgttg aagcgttcga cgagcttcaa ctggcggctc gcttacgcct tgccgttcag
      241 gaacaagggg ttcgagctgt ttctccacgt cgacaatgtg gccgatactt tcatcgagcc
      301 gcagctgggc cttccggctc ccggccgctc gttccgcgtc ggcttgcggt tggattgagg
      361 tcctcgtttc cccgactcag cggcaatatg ggggccgctg cagagtgaga acgatagtag
      421 aacaaatcgc tgtcctacag cgcctctcct gagtcataaa ctggccggtt tggccacgac
      481 tcgaggagac ttcccatgca caacgacacg tccggaccgg ccccggcgtc ttccgacgtt
      541 caccggcccg acgatgtccc cggctttctc cgttttgcgc cggtgccggt gcgggcgcgg
      601 cgggacgggt ggaccgccga gcgtcagctc cgtttcatcc tcgctctcgc ccgcggcgcg
      661 agggccggtg aggcggcgcg gcggctcggc cgctcgcggc agagcgccta tgcgctgcgc
      721 cggcgcgcgg gggccgaagc gttcgcagcc gcctgggacg gggcggtgga cttcgccgcc
      781 gaggcgcgtg gcgctccagg gatgccgggc gcttcttcct cgggcatcga gacattgctg
      841 gtgccgcgtt actatcgagg gcggctgatc ggcttcgtcc agcgcgagga tctgcgcggc
      901 ctgatggcgc ggctcagccg gctggaccgt atggccgagc ggctggaggc gcgggcggcc
      961 ggaagatgac aaggatgaca tgatggcctt gccagacctt gtgctttgtc gcctttgcgt
     1021 gggtagtcgt gcattgctcg aaaggatggc gtctcagccc gcctttgcca tctccagcac
     1081 cgccgcccat tcctcctcgc ggactgggct gaccgaaagc cgggactggc ggatgagctc
     1141 catcttggcg aggcgcggct ccgccttgat cgctttcaag gtgacggggc cgagcggccg
     1201 gaccggctcg accggcacct tcacccaggc gccgtccgcg ccgtcggggc tgccctcgcc
     1261 cgccagccgc atgattccga ccaccgcccg ctcgtccccg ctatggtaga agaaggcctc
     1321 gtcgcccgcc ttcatcgctc ggaggtggag ccgggcggcg gggttgcgca ccccgtccca
     1381 gtcggtgcgc ccgtcgcgga cgagatcgtc ccagctatag gtcccgggtt cggacttcat
     1441 cagccaataa gccatgccgc atcccgtaga tttttccgcg cgtccgcgcc agagagggct
     1501 gatgaggcgc gcccttctcc ttctcgttct ttccctcgcc gcttgcggag agccggtg
//
LOCUS       DASWNU010000191         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_500433, whole genome shotgun sequence.
ACCESSION   DASWNU010000191 DASWNU010000000
VERSION     DASWNU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500433"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VGB57_03800"
     CDS             complement(<1..1033)
                     /locus_tag="VGB57_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEY0130505.1"
                     /translation="MNGCTPLPPGSGFIQWILAFLDCQSWSLGARGWQALAAPGSTLT
                     VLLLGFLTLFVALFGYRLLLGQGPDVRTGVIAFVKIGIVFALATSWGAYSTLVYDVVN
                     RGPAQLATEIGTVSGLPGTGGDLVARLDGADRAFVALTVLGEGRPPPGFDANQSAAAN
                     IPPQPYPGFNTYGIAASRMLFLIAAIAGLGAVRLIAGLLLAVGPFFIAFLLFENTRSL
                     FEGWVRVLAGAALAALGISIALAVELALIEPWLTDLLARRAADEWLPGMPVELLVVSF
                     AFALIVLAMLYASAKTVMAFRLAPVWQAVTAGAGRNPASEERRFAVAATARGDAPPSE
                     ARSRAATVAD"
     gene            complement(1033..3405)
                     /locus_tag="VGB57_03805"
     CDS             complement(1033..3405)
                     /locus_tag="VGB57_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEY0130506.1"
                     /translation="MQLLPPLTRDRRAPSRELPAGKHLPYARHLDDHTIETRDGLLIQ
                     VLQLRGLLFETADTEEINYRKRLRDAMLQSIGSSRYAIYHHVVRRRIDVGLSEGHPDP
                     FSRQLDAAWRRRLDGKRLYINELFLTLVRRPLQGRIGRLDRLRERLGRASAAPDAGAA
                     YERHQLGQARDALMAALGSYEPRLLGVYETPHGPCSEPLEFLSMLYNGEMRPVLLPLQ
                     DLGDYLPYRRISFGQETLELGPAGATQRSFQGLVSIKDYPGQTAAGMLDELLRLPFEL
                     TVSQSFGFVERQAALSTMNLALRRMRSAEDEAVSLRAELSNAKDEIAAGRAGFGEHHM
                     TIALRGDTPAAVAEGIAEVQASLADLGIISVREEIALEPAFWAQFPGNFKYIARRGLI
                     STGNFAGLASGHNFALGRAQGNHWGEAVTLLETTAAGPYYFNFHQGDLGNFTVIGPSG
                     SGKTVVLNFLLAQARKFRPRIIFFDKDRGAELFIRAIGGRYDLLRAGTPSGLNPLQLP
                     DNAANRQFLIDWVAMLAGGADIDEIARIKDAIDANFEQPEDHRRLRHLAELFRGGHRP
                     TASDLWSRLRPWWGDGERAWLFDNARDRTDLTAESVGFDMTQILDDPALRTPAMMYLF
                     HRVEERLDGSAAIIVVDEGWKALDDDVFVRRIKDWEKTIRKRNGIVGFATQSAQDALE
                     SRIASAIVEQAATQIFMANPKARAEDYVGGFGLTPHEYELIRTLPDSAHCFLIKHGTD
                     SVVARLNLSGEQDLLTILSGRERTVRLLDEIRAQTGDDPAEWIPRLLEVA"
     gene            complement(3384..3668)
                     /locus_tag="VGB57_03810"
     CDS             complement(3384..3668)
                     /locus_tag="VGB57_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB3"
                     /protein_id="HEY0130507.1"
                     /translation="MDGLEHDRLFVALTRPQMFAGVTYSYFIANAIVATELFLIFKSI
                     WAILAALVIHVVGVLLCLREPRFFDLWLTRVSRCPRVKNHKVWGCNSYRP"
     gene            complement(3672..3995)
                     /locus_tag="VGB57_03815"
     CDS             complement(3672..3995)
                     /locus_tag="VGB57_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY0130508.1"
                     /translation="MLRGWFGAALATGLMAWSSAALAQDSYSGADPAGSGVIVSAVRW
                     LQGTLLGTVATVVAVIAVASVGFLMLTGRINWRYGATVILGCFILFGAASIVAGIQST
                     SSLGN"
     gene            complement(4184..>4589)
                     /locus_tag="VGB57_03820"
     CDS             complement(4184..>4589)
                     /locus_tag="VGB57_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130509.1"
                     /translation="PEEIERLYRRSQEMGAGRAAAQALLLCERVLATPVPPALVDELR
                     SDRINRWLLSVALRKLAGRTLTAELDEKLLGTGTIHLMQFALLPGLRFKYAELCRQLV
                     NPVDRIAFPLPRALAFLYPLVAMSRRLGRRAP"
BASE COUNT          801 a         1471 c         1678 g          639 t
ORIGIN      
        1 cgtcggcgac ggtggcggcg cgcgagcgcg cttcggacgg aggcgcatcg ccccgggccg
       61 tggcggcgac ggcgaagcgc cgctcctcgc tggccggatt gcggccggcc ccggctgtca
      121 ccgcctgcca caccggcgcg agccggaaag ccatgaccgt cttcgccgag gcgtagagca
      181 tcgccaggac gatgagagcg aaggcgaaag agacgacgag cagctcgacc ggcattccgg
      241 gaagccattc gtcggcggcc cggcgggcga ggagatcggt cagccagggt tcgatcaggg
      301 cgagctcgac ggcgagcgcg atcgaaatgc cgagcgcggc gagcgcggcc ccggccagca
      361 cccgcaccca gccttcgaac agggagcgcg tattctcgaa caacaggaaa gcgatgaaga
      421 agggtccgac cgcgagcagc aggccggcga tcagccggac cgccccaagg ccggcgatcg
      481 ccgcgatcag gaacagcatc cgcgacgccg ctatcccgta ggtgttgaag cccggatagg
      541 gctggggcgg gatgttggcc gcggccgact ggttggcgtc gaagcccggc ggcggccggc
      601 cttcgccgag gacggtgagg gcgacgaagg cacggtcggc gccgtcgagg cgggcaacca
      661 ggtcgccgcc ggtccccggc aggccgctca cggtgccgat ctcggtggcg agctgggccg
      721 ggccgcggtt cacgacgtca tagaccaggg tcgaataagc gccccagctc gtggcgagag
      781 cgaagacgat cccgatcttg acgaaggcga tcaccccggt ccgcacgtcg ggaccctggc
      841 cgagcagaag ccggtagccg aacagggcga cgaagagggt gaggaaaccc aggagcagaa
      901 cggtcagtgt cgatccgggc gcggccagcg cctgccagcc gcgcgcgccc agcgaccagg
      961 actggcaatc gaggaaggcc agaatccact ggatgaagcc cgaccccggc ggaagcggcg
     1021 tgcagccgtt catcaggcga cctccagcag gcgcggaatc cactccgccg gatcgtcgcc
     1081 ggtctgggcg cggatctcgt cgagcagtcg gacggtgcgc tcgcggccgg acaggatggt
     1141 gaggaggtcc tgctcgccgc tgaggttgag ccgggcgacg acgctgtcgg tgccgtgctt
     1201 gatgaggaag caatgggcgc tgtccggaag ggtccggatc agctcatatt cgtgcggagt
     1261 gagaccgaag ccgccgacat aatcctcggc ccgcgctttc ggattggcca tgaagatctg
     1321 ggtcgcggcc tgctctacga tcgcgctcgc gatccggctc tcgagggcgt cctgggcgct
     1381 ctgggtggcg aagccgacga tcccgttgcg ctttcggatc gtcttctccc aatccttgat
     1441 ccggcggacg aagacgtcgt cgtcgagcgc cttccagccc tcgtcgacga cgatgatcgc
     1501 ggcgcttccg tcgagccgct cctcgacccg gtggaagagg tacatcatcg ccggagttcg
     1561 aagcgccgga tcgtcgagga tctgggtcat gtcgaagccg acggattcgg cggtgaggtc
     1621 ggtgcggtcg cgggcgttgt cgaacagcca ggcgcgctcg ccgtcgcccc accagggccg
     1681 gagccgcgac cacagatcgc tggcggtcgg gcgatggccg ccgcggaaca gctcggcgag
     1741 gtggcggagc cggcgatggt cctcgggctg ctcgaaattg gcgtcgatcg cgtcctttat
     1801 ccgggcgatc tcgtcgatgt cggcgccgcc ggcgagcatg gccacccaat cgatcaggaa
     1861 ctggcggttg gccgcgttgt cgggaagctg gagcggattg aggcccgaag gcgtgcccgc
     1921 ccgaagcagg tcgtagcgcc ctccgatcgc gcggatgaag agctcggcgc cgcgatcctt
     1981 gtcgaagaag atgatccggg gacggaactt gcgcgcctgg gcgagcagga aattgaggac
     2041 caccgtcttg cccgagcccg acggcccgat gacggtgaaa ttgccgaggt ccccttggtg
     2101 gaaattgaaa taataggggc ccgccgccgt cgtctcgagg agggtcaccg cctctcccca
     2161 atgattgccc tgggcgcgcc cgagcgcgaa attatggccg ctggcgaggc ccgcgaaatt
     2221 gccggtcgag atcagcccgc gacgggcgat atatttgaag ttgccgggga actgcgccca
     2281 gaaagcgggc tcgagagcga tctcctcgcg caccgagatg atgccgagat cggccagcga
     2341 cgcctgcacc tcggcgatgc cctcggcgac ggcggcgggg gtgtcgccgc gcagggcgat
     2401 cgtcatgtga tgctcgccga agccggcgcg cccggcggcg atctcgtcct tggcattgga
     2461 gagctcggcg cgaaggctca ccgcctcgtc ctcggccgag cgcatccgcc tcagggcgag
     2521 gttcatggtc gaaagcgccg cctgccgctc gacgaagccg aaggactggg agacggtgag
     2581 ctcgaacggc agtcgaagga gctcgtcgag catcccggcg gcggtctggc cgggataatc
     2641 cttgatcgac acaagtccct ggaagctgcg ctgggtcgct ccggccgggc cgagctccag
     2701 cgtctcctgg ccgaagctga ttcgccggta ggggagatag tcgccgaggt cctggagggg
     2761 caggagcacc ggcctcatct cgccattgta gagcatggag aggaattcga gcggctccga
     2821 gcagggcccg tgcggggtct cgtacacgcc gagcaggcgc ggctcgtagc tgccaagggc
     2881 cgccatcaat gcgtcgcgcg cctggccgag ctggtggcgt tcgtaggcgg cgccggcgtc
     2941 cggggcggcg gaggcgcggc ccagccgttc gcgaagccgg tcgagccggc cgatccggcc
     3001 ctgcagcggc cgccgaacga gggtcaggaa gagctcgttg atgtagaggc gcttgccgtc
     3061 gaggcggcgg cgccaggcgg cgtcgagctg gcgcgagaag ggatcgggat ggccctcgga
     3121 caggccgacg tcgatcctcc gccggacgac gtgatggtag atcgcgtagc gcgacgaacc
     3181 gatcgactgg agcatcgcgt cgcgcagccg cttgcgataa ttgatctcct ccgtgtcggc
     3241 cgtctcgaac agcagtccgc gcagctggag gacctggatc agcaggccgt cccgggtctc
     3301 gatcgtatgg tcgtccagat gacgcgcgta ggggaggtgc ttgccggccg gcagctccct
     3361 cgacggcgct cgccggtcgc gggtcagggg cggtaggagt tgcatcccca caccttgtgg
     3421 ttcttgacgc gcgggcagcg cgagactcgg gtcagccaaa ggtcgaagaa gcgcggctca
     3481 cgaaggcaca gcaacactcc gacgacgtgg atgacgagcg cggcgaggat cgcccagatc
     3541 gacttgaaga tcaggaacag ctcggtcgcg acgatcgcgt tggcgatgaa ataggaatag
     3601 gtcacgcccg cgaacatctg cgggcgcgtc agggcgacga acagccggtc atgttcgagc
     3661 ccgtccacgc ctcagttgcc gaggctggac gtggactgga tcccggcgac gatgctcgcc
     3721 gcgccgaaga ggatgaagca gccgaggatg acggtcgcgc cgtagcgcca gttgatccgg
     3781 ccggtcagca tcaggaagcc gacggaggcg acggcgatca ccgccaccac ggtcgccacg
     3841 gttccgagca gggtcccctg cagccatcgg acagcggaca cgatcacccc cgagccggcg
     3901 ggatcggcgc ccgaatagga atcctgcgcc agggcggccg aagaccaggc catcagcccc
     3961 gtcgccaaag ccgcgccaaa ccaaccacgt agcaatccaa tcacccggta cactccccac
     4021 cctgtcgaat cgcgggcatc cccgatgcgg atattggact gttgtcagag acttgccaat
     4081 agtcgacgcc atcggggccc aaaggcccgt tcccgcgcac cttacgcctt gcggcccccg
     4141 gcaggaagcc ttgtgggaaa ttcggcccgg ggcggggccg tcgctatggc gcgcgccggc
     4201 ccagccggcg cgacatcgcg accagcggat agaggaaagc gagcgcccga ggcaggggaa
     4261 atgcgatccg gtcgacggga ttgaccagct ggcggcagag ctcggcatat ttgaaccgga
     4321 ggccgggcag aagcgcgaat tgcatgaggt ggatggtgcc ggtgccgagc agcttctcgt
     4381 cgagctcggc ggtgagggtg cgcccggcaa gcttgcgaag cgcgaccgac aggagccagc
     4441 ggttgatccg gtcggagcga agctcgtcca cgagagcggg cggcaccggg gtggcgagca
     4501 cccgctcgca cagcagcaag gcctgggcgg ccgcgcgccc cgcccccatt tcctgcgagc
     4561 ggcggtagag ccgctcgatc tcttcaggg
//
LOCUS       DASWNU010000192         6683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_292270, whole genome shotgun sequence.
ACCESSION   DASWNU010000192 DASWNU010000000
VERSION     DASWNU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292270"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2376
                     /locus_tag="VGB57_03825"
     CDS             <1..2376
                     /locus_tag="VGB57_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0130510.1"
                     /translation="FPMFVVWGPDLRFLYNDAYVEILGDKHPFALGFAFEDIWADVWE
                     QIRPILERALAGEGSFYENLPLTMTRRGFSEEAWFTFSYTPLRDEAGAVGGMLCTVVE
                     TTATVLAERERLNETERLRQLFHNAPGFMAVVRGPDHVFEMANASYQKLVGRRDLIGK
                     TVREAVPEVEGQGLFELLDKVYRSGEPFSGRSVSVRLQRRPDGPREERLLDFIFQPIF
                     DAQGAVSGIFADGYDVTERIVAETALRESEERFRLIADSAPVPMWVTKLDRKRSFVNR
                     AYVEFLGISYDEAVDFDWRTVIHPDDSARVVAESMAGEASLETFELVARYHSGGEWRW
                     VRSVSQPRWGPQGEHCGFIGVAHDITELKRAEAALREVNETLERRVSERTADLSAALD
                     RLQAEVGERLKAEEALRQAQKMEAVGQLTGGIAHDFNNLLTPIMGGLEMIASRVEDAR
                     LKRIAETALESTRRGAKLTGQLLAFSRIQRISMAPLAVNAVIDGMQRLLRHTIGGEVR
                     IETRLHPGAGHGICDANQLENAILNLAINARDAMPDGGTLTICTERVRLDEAPDHPAG
                     EFVRITVADTGFGMPPDVLARATEPFYSTKPLGKGTGLGLAQVYGIAQQAGGTLRIES
                     EEGKGTSVHILLPAAAVPAEAEAELSGRSSPSPQGAAAGTSLLVVDDDSDVRGFLAES
                     LEGLGCTVVSAASGTEGLRAMREWRPDLVLMDYAMPGMNGADAARAARELHPGLPIVF
                     VTGYAESEQLEAALGCDVPVLRKPFTLDQLAAAVEENVLPPHAQHGEGDHP"
     gene            2642..3118
                     /locus_tag="VGB57_03830"
     CDS             2642..3118
                     /locus_tag="VGB57_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY0130511.1"
                     /translation="MRSMDFTPYRRNTVGFDRLFDFLESARSEQSDNYPPYDIEKLSD
                     DRYRITLAVAGFSSADIDITARQNMLVVVGRKSEDRGKDGSFLHMGIATRAFERRFEL
                     ADFVRVESADMKDGLLSIELVREIPDAMKPRKIEIGSGRSQTIEAQAAPRKEPEVA"
     gene            3304..3465
                     /locus_tag="VGB57_03835"
     CDS             3304..3465
                     /locus_tag="VGB57_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130512.1"
                     /translation="MRFLKKLTNPFVLVAEGFVVGAILFATSTPNLVDSPAAPPPALD
                     SSVIPNSGR"
     gene            3504..4166
                     /gene="pdeM"
                     /locus_tag="VGB57_03840"
     CDS             3504..4166
                     /gene="pdeM"
                     /locus_tag="VGB57_03840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY0130513.1"
                     /translation="MVRFSFCGQELMALPQGALYWPSRRALLVADLHLEKASWFARMG
                     QMLPPYDSIATLADLTALAVSTDAREIWCLGDSFHDRHGCDRLPARAREMLTALTAAT
                     RWTWITGNHDPGIADHCGGEIVEEAEVQGLLLRHEAEPAEPRPELSGHFHPKLRIHHR
                     GRQVSRRCFVATERKLILPAFGSLTGGLDAGHPEIVRAVGPKAEALVPVADRLLRFPL
                     AA"
     gene            complement(4280..6010)
                     /gene="pabB"
                     /locus_tag="VGB57_03845"
     CDS             complement(4280..6010)
                     /gene="pabB"
                     /locus_tag="VGB57_03845"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY0130514.1"
                     /translation="MLEFDGRARLYRRPAEVVEAREPGAVKPALDRLRGRHSAGFMAY
                     EAGHGLEPRLEPLRREAEPGGPPLLWFGLFEGWEEIDPAALLPDPAGARASRPRPLVE
                     RGAHEAAVGRALEHILAGDIYQANLTFPAEVATAGHPLALYAAIRPRARAGWGGVVFT
                     GEHWLLSFSPELFFTLEAGRVTARPMKGTAPAGSDPEALRSDPKQRSENLMIVDLLRN
                     DLSRLSRPGTVKVPALFEVETYPTVLQMTSTVTGELEDGQTGVDLIETIFPCGSVTGA
                     PKIRAMEIIASLEGERRGPYTGSIGRLAPDGDAAFNVAIRTLVLRGGETVARLGLGSG
                     IVADSIAADEWRECLAKGEFVATGRRFDLIETMRFDPREGLYELDRHLARMKRSADAF
                     GFAFDRHHARNELQAATFFLRQARKLRLLLSSSGALAIESRPLAAAPEGPVEVALAPL
                     PVESEDFRLGHKTSDRDFYDDAREATGTFEVVFVDRDGFLTEGSFTSLFVERGGRLLT
                     PPVTRGLLPGVLREILLEEGRAEEADLRAEDLEAGFFVGNAVRGLMRAQLASPSQRDG
                     EGTMRSMVEG"
     gene            6081..>6683
                     /locus_tag="VGB57_03850"
     CDS             6081..>6683
                     /locus_tag="VGB57_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HEY0130515.1"
                     /translation="MVTSVFDLFKIGVGPSSSHTMGPMTAACRFVEALSQKGLLDRTE
                     RVLAELYGSLALTGKGHATDRAILLGLSGERPDRMDPDAADEVIAAVRSTGRLRLGGA
                     REIAFDEPHDLKFLQRERLPHHSNGMRFTAYDGAGEPLESSVYYSTGGGAVVDEAAIS
                     RNAPPEGFWDIPYNYRCADELLAIAEREGLTIADIARANER"
BASE COUNT         1053 a         2407 c         2184 g         1039 t
ORIGIN      
        1 ttcccgatgt tcgtggtctg ggggccggac ctgcgcttcc tctacaacga cgcgtatgtg
       61 gaaatactgg gcgacaagca cccgttcgcg ctgggcttcg ctttcgagga catctgggca
      121 gacgtctggg agcaaatccg cccgatcctc gagcgtgctt tggccggaga gggcagcttc
      181 tacgagaacc tcccgctgac tatgacccgc cgcggctttt ccgaggaggc gtggttcacc
      241 ttttcctaca ctccccttcg ggatgaggcg ggcgccgtcg gtggaatgct ctgcaccgtc
      301 gtcgaaacca ccgcgaccgt cctcgccgag cgcgagcgcc tgaacgagac cgagcgcctc
      361 cgccagctgt tccacaacgc gcccggcttc atggccgtgg tgcgcggccc cgatcatgtc
      421 ttcgagatgg ccaacgcttc ctaccagaag ctggtcggac gccgcgacct gatcggcaag
      481 accgttcggg aggccgtccc ggaggtcgag ggccagggac tcttcgagct gctcgacaag
      541 gtctatcgca gcggcgagcc cttttccggc cgatcggtat cggtccggct ccagcgccgc
      601 ccggacggtc cgcgcgagga gcggctgctc gatttcatct tccagccgat cttcgacgcg
      661 cagggcgccg tcagcggcat cttcgccgac ggctatgacg tcaccgagcg gatcgtcgcc
      721 gaaaccgcgc ttcgggaaag cgaggagcgc tttcgcctga tcgcagactc ggcgccggtg
      781 ccgatgtggg tgaccaagct cgaccgcaag cgaagcttcg tcaaccgcgc ctatgtcgag
      841 ttcctcggca tcagctacga cgaggccgtc gacttcgact ggcgcaccgt catccatccc
      901 gacgactcgg cgcgggtggt cgccgaatcg atggccggcg aggcctcgct cgagacgttc
      961 gaactggtcg cgcgctatca cagcggcggc gagtggcgct gggtccgctc cgtctcgcag
     1021 ccgcgctggg gcccccaggg cgagcattgc ggcttcatcg gggtcgccca cgacatcacc
     1081 gagctgaagc gggccgaagc ggcgcttcgc gaggtcaacg aaacgctcga gcggcgggtc
     1141 tcggagcgca ccgccgatct cagcgccgcc ctcgaccggc tccaggccga ggtcggcgag
     1201 cgcctcaagg cggaggaggc gctccgccag gcccagaaga tggaggcggt cggccagctg
     1261 accggcggga tcgcgcacga tttcaacaat ctcctcaccc cgatcatggg cgggctggag
     1321 atgatcgcct cgcgggtgga ggacgccagg ctcaagcgga tcgccgagac cgcgctcgaa
     1381 tcgacccggc gcggcgccaa gctcaccggc cagcttctcg ctttctcgcg gatccagcgg
     1441 atcagcatgg cgccactcgc cgtcaacgcc gtcatcgatg ggatgcagcg cctgcttcgc
     1501 catacgatcg gcggcgaggt caggatcgag acccgtctcc atcccggcgc cgggcacggc
     1561 atctgcgacg ccaatcaact cgagaatgcg atcctcaacc tcgccatcaa cgcccgcgac
     1621 gccatgcccg acggcggcac cctgaccatc tgcaccgagc gcgtccggct ggacgaggcg
     1681 ccggaccatc ccgcgggcga gttcgttcgg atcaccgtcg ccgacaccgg cttcggaatg
     1741 ccgccggacg tgctcgcccg ggccaccgag cctttctatt cgaccaagcc gctcggcaag
     1801 gggaccggcc tcggcctcgc ccaggtctac gggatcgccc agcaggcggg cgggaccctg
     1861 cgcatcgaaa gcgaggaggg caagggtacg tccgtccaca tcctgctccc ggccgccgca
     1921 gttccggcgg aggccgaggc cgagctttcc ggccgctcca gcccctcgcc gcagggcgcg
     1981 gcggccggaa cgagcttgct ggtcgtcgac gacgattccg acgttcgcgg cttcctcgcc
     2041 gaatcgctcg agggtctcgg ctgcaccgtg gtctcggccg cgtcggggac ggaggggctt
     2101 agggccatgc gcgaatggcg gccggatctc gttttgatgg actatgcgat gcccggaatg
     2161 aacggggccg acgccgcccg cgccgcgcgc gagctccatc ccggcctgcc gatcgtcttc
     2221 gtcacgggct atgccgaaag cgagcagctc gaggcggccc tgggctgcga cgtgccagtg
     2281 ctccgcaagc ccttcaccct cgaccagctg gcggcggcgg tggaggagaa tgtcttgcct
     2341 ccccatgcgc agcatgggga gggggaccac ccgtaagggt ggtggagggg tagaaggctg
     2401 tgattctggc ggttcgcgcg aaatccgccc atttcagcgc caattacccc tcaccaagct
     2461 tcgcttggtc cccctcccca tcgcttcgcg acagggaggc aaaaggggcc ttgaaccccg
     2521 cagatcctca cctagatcgc cgtcgtcgga cgacccgtca ggtgtccggc ctgaatccgc
     2581 gaggggcgat gcgctctttg ccgccgctcc tcacgatgtt gctcaaacgg aggacattga
     2641 catgagaagc atggatttca ccccctatcg ccgaaacacc gtcggcttcg accgcctctt
     2701 cgatttcctc gaaagcgcgc gctccgagca gtccgacaat tatccgccct acgacatcga
     2761 gaaactgagc gacgaccgct accggatcac cctcgccgtc gccggcttca gttccgcgga
     2821 catcgacatc accgcccgcc agaacatgct ggtcgtggtc ggccgcaagt cggaggaccg
     2881 gggcaaggac ggcagcttcc tgcacatggg gatcgccacc cgcgcgttcg agcgccgctt
     2941 cgagctcgcc gatttcgtcc gggtcgagtc cgccgacatg aaggacggtt tgctctcgat
     3001 cgagctggtc cgcgaaatcc ccgacgcgat gaagccgcgc aagatcgaga tcggaagcgg
     3061 ccggtcgcag acgatcgagg cccaggcggc gccccggaag gagcccgagg tcgcgtaagt
     3121 cttgcctccc catcgaagat ggggaggggg accccgcgta gcggggtgga ggggtgatgg
     3181 cgcaggcctg ttccagtcac ccctccacca cccttcgggt ggtccccctc cccgtcgctt
     3241 cgcgacaagg aggcaagata tcgattcatc ccacgtttac ccgcgaaggc gcactccgtc
     3301 ccgatgcgtt tcctgaaaaa gctcaccaac ccgttcgtac tcgtcgccga aggcttcgtc
     3361 gtcggcgcca tcctcttcgc gacctcgacc ccgaacctcg tcgactcgcc ggccgcgccg
     3421 ccgcccgcgc tcgattcctc ggttattcca aactccgggc gctgaaccct tgaacctgcg
     3481 ggccgcccgg gtatagcggg cccatggttc gcttttcgtt ctgtggccag gagctgatgg
     3541 cgcttccgca gggggccctc tactggccgt cgcgccgggc cctgctggtc gccgacctcc
     3601 atctcgagaa agcgagctgg ttcgcccgca tggggcagat gctcccgccc tacgattcca
     3661 tcgccaccct ggccgatctg accgcgctcg ccgtctcgac cgacgccagg gaaatctggt
     3721 gtctcggcga cagcttccac gaccgccacg gctgcgatcg cctccccgcc cgggcccgcg
     3781 agatgctgac ggcgctcacg gccgcgacgc gctggacctg gatcaccggc aatcacgatc
     3841 ccggaatcgc cgaccattgc ggcggcgaga tcgtcgagga ggcggaggtg caaggcctgc
     3901 tgcttcgcca cgaggcggag ccggccgagc cgcggcccga gctgtccggc catttccacc
     3961 ccaagctccg gatccaccat cgcggccgcc aggtctcgcg ccgctgcttc gtcgcgacgg
     4021 agcgcaagct gatccttccc gccttcggct cgctgacggg ggggctcgat gcaggccatc
     4081 ccgagatcgt ccgcgcggtg gggcccaagg cggaggctct ggtgccggtc gccgaccggc
     4141 ttttgaggtt cccgctggcg gcttgatttg cctccctgcc ggcggagtcg gcggggaggg
     4201 ggaccacccg aagggtggtg gaggggtagc tgccgtggac caaggaggtt cacgtgcgaa
     4261 ccgcccgcag ccgcgccttc tacccctcca ccatgctacg catggtcccc tccccatcgc
     4321 gctgcgatgg ggaggcaagt tgcgccctca tcaaaccccg caccgcattc cccacgaaaa
     4381 atcccgcctc cagatcctcc gcccgcaaat ccgcctcctc ggcccgcccc tcctccagca
     4441 gaatctcccg aagcaccccc ggcaatagcc cccgcgtcac cggcggggtc agcagccggc
     4501 cgccgcgctc gacgaacagg ctggtgaagc tgccctcggt caggaagccg tcgcggtcga
     4561 cgaaaacgac ctcgaacgtc ccggtcgcct cgcgggcgtc gtcgtagaaa tcccggtcgc
     4621 tggtcttgtg gccaaggcgg aaatcctcgg actcgaccgg aagcggcgcc agagccacct
     4681 cgaccggccc ttcgggcgcc gccgccagcg gccgcgattc gatcgccagg gcgccgctgc
     4741 tcgacaacag gagcctgagc ttgcgcgcct gccgaaggaa gaaggtcgcc gcctggagct
     4801 cgttgcgcgc atggtggcgg tcgaaggcga agccgaaggc gtcggcgctc cgcttcatcc
     4861 gggcgaggtg acggtcgagc tcgtacaggc cttcgcgcgg gtcgaagcgc atcgtctcga
     4921 tgaggtcgaa gcgtcggccc gtcgccacaa actctccctt cgccaggcat tcgcgccact
     4981 cgtccgccgc gatgctgtcc gcgacgatcc cggagccgag gccgagccgg gcgaccgtct
     5041 cgccgccccg gaggaccagg gtccggatcg cgacattgaa cgcggcatcg ccgtccggcg
     5101 caagcctgcc gatactaccg gtataggggc cccgtcgttc cccctccaga gaagcgatga
     5161 tctccatagc cctgatcttg ggcgccccgg tcaccgatcc gcaggggaag atggtctcga
     5221 tcaggtcgac ccccgtttgc ccgtcctcga gctcgccggt cacggtcgac gtcatctgga
     5281 gcacggtcgg ataggtctcg acctcgaaca aagccggcac tttcacggtc cccggcctcg
     5341 acaggcgcga gaggtcgttg cgaagcaggt cgacgatcat caggttttcg gagcgctgct
     5401 tggggtcgct ccgaagcgct tcgggatcgc tgcccgcggg cgccgtcccc ttcatcggcc
     5461 gagcggtcac ccgccccgcc tccagggtga agaacaattc cggggagaag gagagcagcc
     5521 aatgctcgcc ggtgaagacc acgccgcccc atcccgcccg ggcccgcggc cggatcgcgg
     5581 cgtagagggc gagcggatgg ccggcggtcg cgacctcggc tgggaaggtg agattggcct
     5641 gatagatgtc gccggcgagg atatgctcga gcgctcgtcc caccgccgcc tcgtgcgccc
     5701 cgcgctccac cagcggccgg ggccgggacg cccgggcgcc cgcggggtca ggcaggaggg
     5761 cggccgggtc gatctcctcc cagccctcga acagcccgaa ccagagcaag ggcggcccgc
     5821 cgggttcggc ctcgcgccgc agcggctcca gcctgggctc gaggccatgc cccgcttcat
     5881 aggccatgaa tcccgccgag tgccggcccc gcagccggtc cagcgccggc ttcaccgctc
     5941 cgggctcccg cgcctccacc acctcggccg ggcgccggta gaggcgggcg cgcccgtcga
     6001 actcgagcag gacgaacggt tcaccggctt gccagaagcg cgaagcgggc ccatctgccg
     6061 gggcgtcagg aggtaccttc ttggtcacca gcgtcttcga cctcttcaag atcggggtcg
     6121 gcccgtccag ttcgcacacg atggggccga tgacggccgc gtgccgcttt gtcgaggcgc
     6181 tgtcgcaaaa gggcctgctc gaccggaccg agcgggtgct cgccgagctc tacggatcgc
     6241 tggctctgac cggcaagggc cacgccaccg accgcgccat cctgctcggc ctgtcgggcg
     6301 agcggcccga ccggatggac ccggacgcgg ccgacgaagt cattgcggcg gtccgctcga
     6361 cagggcgcct gcgcctcggc ggggccaggg aaatcgcctt cgacgagccc catgacctca
     6421 agttcctcca gcgcgagcgg cttccccacc attccaacgg aatgcgcttc accgcctatg
     6481 acggcgccgg cgagccgctc gaatcgtcgg tttattattc gaccggcggc ggcgccgtgg
     6541 tcgacgaggc ggcgatcagc cgcaacgcgc cgcccgaagg cttctgggac ataccctaca
     6601 attaccgctg cgccgacgag cttctcgcca tcgccgagcg cgaggggctg accatcgccg
     6661 acatcgcccg tgccaacgag cgg
//
LOCUS       DASWNU010000193         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_292292, whole genome shotgun sequence.
ACCESSION   DASWNU010000193 DASWNU010000000
VERSION     DASWNU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292292"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..775
                     /locus_tag="VGB57_03855"
     CDS             158..775
                     /locus_tag="VGB57_03855"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY0130516.1"
                     /translation="MAKLYFYYASMNAGKSTVLLQTAFNYRERGMEPMLWTAAVHDRS
                     GFGIIDSRIGITAQANAYEPGTDLRREVQAELAKRDVHCILVDEAQFLSRDQVMQLAA
                     ICDELDIPVLAYGLRTDFQANLFEGSATLLALADKLIELKAICECGRKATMNLRVDQT
                     GAAVASGAQTEIGGNDRYVALCRRHFMERIAEAEAKQLRLSLVGG"
     gene            780..1673
                     /gene="truB"
                     /locus_tag="VGB57_03860"
     CDS             780..1673
                     /gene="truB"
                     /locus_tag="VGB57_03860"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0130517.1"
                     /translation="MHGWLVIDKPVGPGSTDIVSRVKRALRDGGYPKLKVGHGGTLDP
                     LASGVLPVALGEATKLAGRMLDADKVYEFTIRFGEETDTLDAEGAVVARSETRVEDAD
                     VEDVLVDFEGVIEQVPPAYSALKVGGKRSYDLARAGAEVTLAERQVTVHELLYRGSWR
                     DDGLLHATLMARVSKGTYIRSLARDIARALDSVGHVTMLRRLKAGPFTLASAISLDKM
                     GELAKARQLEQALLPLTAGLDDIPALPVTPDQAGLLRQGRKLAGIAAKPGLHLATESS
                     VPVALVELSEGELRVVRGFNI"
     gene            1689..1958
                     /gene="rpsO"
                     /locus_tag="VGB57_03865"
     CDS             1689..1958
                     /gene="rpsO"
                     /locus_tag="VGB57_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0130518.1"
                     /translation="MSVTAERKQEIIEDNARGSGDTGSPEVQVAILTERINNLTAHFK
                     THAKDNHSRRGLLMMVNKRRSLLDYIRKEDHARYTALIAKLGLRK"
     gene            2114..>4419
                     /gene="pnp"
                     /locus_tag="VGB57_03870"
     CDS             2114..>4419
                     /gene="pnp"
                     /locus_tag="VGB57_03870"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0130519.1"
                     /translation="MFDMKKVEIEWGGKTLTLETGRVARQADGAVLATLGETVVLCAV
                     TAAKNVKPGQDFFPLTVHYQEKFSAAGRIPGGFFKRERGATEKETLTSRLIDRPIRPL
                     FPEGFYNEVLVIAHVMSYDGENEPDVVAMIAASAALTLSGIPFMGPIGAARVGYANGE
                     YILNPTQDQIKEGELDLVVAGTHNAVMMVESEAKELSEDVMLGGVMFAHRSIQPVIQG
                     IISLAEKAAKDPWELEEPTDTKAIKEKLRGIIGEDLAAAYKLTDKQQRQTAINEARAK
                     AREGLADMEASDPSGYLGALKLVKKLEAEIVRGAILKEGVRIDGRDTKTVRPIEAMVG
                     FLPRTHGSALFTRGETQAICTTTLGTKDAEQMIDGLEGLSYSRFMLHYNFPPYSVGEV
                     GRFGAPSRRETGHGKLAWRALHPVLPSHEDFPYTIRILSDITESNGSSSMATVCGGCL
                     ALMDAGVPITRPVSGIAMGLILEGQDFAVISDILGDEDHLGDMDFKVAGTEQGITSLQ
                     MDIKIAGITEEIMRIALDQASEGRAHILREMAKAMGEVRGELSAHAPRIETMQVDKTK
                     IRDIIGTGGKIIREIVATTGAKVDIDDEGIVKISSSDVSQIEAARRWIQGIVEEPEVG
                     KVYTGKVVNLVDFGAFVNFMGGKDGLVHVSEIKNERVERVADALSEGQEVKVKVLEID
                     PRGKVRLSMRVVDQETGAELEDTRPPREGGDRDRGPRGDGRGDRGPRRDGEGRGDRGG
                     GRDRGPRRDGGGGGGRGGDRDRGPRGEGG"
BASE COUNT          831 a         1450 c         1478 g          660 t
ORIGIN      
        1 cgcaggcgaa tgtcgtcgag cgggtagtgc gcccgcccgt ggagtggctg ctgaggctgt
       61 tcatcggcgc ctagggaccc ctgaggggga aacgctcgag gtcgactgga cggactcgcc
      121 tggctggcgc tgcgacgccg ccgcgctaaa aggcgccatg gccaagctct atttctacta
      181 cgcgtcgatg aacgccggca agtcgacggt gcttctgcaa acggcgttca attaccgcga
      241 gcgcgggatg gagccgatgc tgtggaccgc cgccgttcac gaccgctccg gcttcgggat
      301 catcgactcc aggatcggaa tcaccgccca ggccaatgcc tatgaaccgg gaacggacct
      361 tcgccgcgaa gtccaggcgg agctggcgaa acgcgacgtc cactgcatcc tggtcgacga
      421 ggcgcagttc ctcagccgtg accaggtgat gcagctcgcc gccatctgcg acgagctcga
      481 catcccggtc cttgcctacg gcctgaggac ggatttccag gccaatctgt tcgagggcag
      541 cgccaccctg ctcgcgctcg ccgacaagct gatcgaattg aaggcgatct gcgagtgcgg
      601 gcgcaaggcg acgatgaacc ttcgggtcga ccagacgggc gccgcggtgg cgagcggggc
      661 ccagaccgag atcggcggca acgaccgtta cgtggcgctc tgccgccgcc atttcatgga
      721 gcggatcgcc gaggcggagg cgaagcagct ccgcctcagc ctggtcggcg gctgaagggg
      781 tgcacggctg gctggtcatc gacaagccgg tggggcccgg ctcgaccgac atcgtctcca
      841 gggtgaagcg ggcgctccgc gacggcggct atccgaagct gaaggtcggc catggcggaa
      901 ctttggatcc gctcgcgagc ggggtgcttc cggtggcgct gggcgaggcg accaagctcg
      961 ctgggcggat gctggatgcc gacaaggtct atgagttcac gatccgcttc ggggaggaga
     1021 cggatacgct cgatgcggaa ggcgcggtgg tggcgcgttc cgaaacccgg gtggaagacg
     1081 ccgatgtgga ggacgtgctg gtcgacttcg aaggggtgat cgagcaggtg ccgccggcct
     1141 attcggcgtt gaaagtgggt ggaaagcggt cttacgatct cgcccgagcg ggcgccgagg
     1201 tgacgctggc cgagcggcag gtaaccgtcc atgaactgct ctatcgtgga agctggcgcg
     1261 acgacgggct gctgcacgcc accctgatgg cccgggtatc gaagggcacc tacatccgct
     1321 ccctcgcccg agacatcgcc cgtgccttgg attcggtcgg ccacgtcacc atgttgagga
     1381 ggctgaaggc cggcccgttc accctcgctt ccgcgatttc gctggacaaa atgggcgaac
     1441 tcgctaaggc gcggcagctt gaacaggcac tcttgccgct gacggcgggg ctggacgaca
     1501 tcccggctct acccgtcacc cccgaccagg caggactgct ccgacagggg cggaaattgg
     1561 ccggaatcgc cgcaaagcca gggcttcatc tagcaaccga atcgtctgtt ccggttgcgc
     1621 ttgtggagct ttccgaaggt gaacttcggg tggtgcgcgg cttcaacatc tagacgaagg
     1681 agttcacgat gtcggttacc gccgagcgca agcaggaaat catcgaagac aatgcccgcg
     1741 gatcgggcga caccggatcc cccgaggtcc aggtcgccat cctcacggag cgaatcaaca
     1801 accttaccgc ccatttcaag acccacgcga aggacaacca ttcccgccgc ggtctactga
     1861 tgatggtcaa caagcgccgc tccctgctgg attatatccg caaggaagat cacgcgcgct
     1921 acaccgcact catcgcgaag ctcgggcttc gcaaataaaa gccggagcgg cccccaacgg
     1981 ggccgcttcg cgtatcgggc tcccgcaatt cggcggcggt ccggtccggg tcatctggac
     2041 ccaaatgccg ggcgacgagc ccgggaaata ggcccccagc gcatctggcg ctgggtgttg
     2101 aaggaaactc agaatgttcg acatgaagaa agtggaaatc gagtggggcg gcaagacgct
     2161 cactctcgag acgggccggg ttgcccgcca ggccgacggc gcggtgctcg cgaccctcgg
     2221 cgagaccgtc gtcctttgcg cggtgaccgc tgcgaagaac gtcaagccgg ggcaggattt
     2281 cttcccgctg accgtgcact accaggaaaa attctcggcc gccgggcgaa tcccgggcgg
     2341 cttcttcaag cgcgagcgcg gcgcgacgga aaaggaaacc ctcaccagcc gcctcatcga
     2401 ccggccgatc cggccgctgt tcccggaggg cttctacaac gaggttctcg tgatcgctca
     2461 cgtcatgtcc tatgacggcg agaacgagcc ggacgtggtg gcgatgatcg cggcttccgc
     2521 ggcgctcacc ctctccggaa ttcccttcat gggcccgatc ggagcggcgc gcgtcggcta
     2581 tgcgaatggg gaatatatcc tcaaccccac ccaggaccag atcaaggaag gcgaattgga
     2641 cctcgtcgtc gccggcaccc acaatgccgt gatgatggtc gaatcggagg ccaaggagct
     2701 ttccgaggac gtcatgctcg gcggagtgat gtttgcccac cgctcgatcc agccggtcat
     2761 ccagggcatc atcagcctcg cggagaaagc cgccaaggac ccgtgggagc tcgaggagcc
     2821 caccgacacc aaggcgatca aggagaagct tcgcggaatc atcggcgagg acctcgccgc
     2881 cgcctacaag ctcaccgaca agcagcagcg ccagacggcg atcaacgaag cccgcgccaa
     2941 ggcccgcgaa ggcctcgccg acatggaagc ctcggacccg tccggctatc tcggcgccct
     3001 caagctggtc aagaagctgg aagcggagat cgtccgcggc gccatcctga aggaaggcgt
     3061 ccggatcgac ggccgcgaca ccaagaccgt ccgcccgatc gaggcgatgg tcggcttcct
     3121 cccccgcacc cacggctcgg cgctcttcac ccgcggcgag acccaggcga tctgcacgac
     3181 cacgctcggc accaaggacg ccgagcagat gatcgacggc ctggaggggc tctcctactc
     3241 gcgcttcatg ctccactata acttcccgcc ctactcggtc ggcgaagtcg gacgcttcgg
     3301 cgctccgtcg cggcgcgaga ccggccacgg caagctcgcc tggcgggcgc tccatccggt
     3361 gctccccagc catgaggact tcccctacac gatccggatc ctgtccgaca tcaccgagag
     3421 caacggctcc tcgtcgatgg cgacggtgtg cggcggctgc ctcgccctga tggacgcggg
     3481 cgtccccatt acccggccgg tctcgggcat cgccatgggc ctgatcctcg agggccagga
     3541 cttcgcggtg atctcagaca tcctcggcga cgaggaccat ctcggcgaca tggacttcaa
     3601 ggtcgccgga acggagcagg gcatcacctc gctccagatg gacatcaaga tcgccggaat
     3661 caccgaggag atcatgcgca tcgccctcga ccaggcgagc gagggccggg cccacatcct
     3721 gcgcgagatg gcgaaggcga tgggcgaggt ccgcggcgag ctttcggccc acgcgccgag
     3781 gatcgagacg atgcaggtcg acaagaccaa aatccgcgac atcatcggca ccggcggcaa
     3841 gatcatccgc gagatcgtcg ccaccaccgg cgccaaggtc gacatcgacg acgagggcat
     3901 cgtcaaaatc tcgtccagcg acgtcagcca gatcgaggcg gcccgccgct ggatccaggg
     3961 catcgtcgag gagcccgagg tcggcaaggt ctataccggc aaggtcgtca acctcgtcga
     4021 tttcggagcc ttcgtgaact tcatgggcgg caaggacggc ctcgtccacg tctcggagat
     4081 caagaacgag cgcgtcgagc gagtggcgga tgccctgtcc gaaggccagg aagtcaaggt
     4141 caaggtcctc gagatcgacc cgcgcggcaa ggtccgactg tcgatgcgcg tcgtcgacca
     4201 ggagaccggc gccgagctgg aggacacccg tcctccccgc gaaggcggcg atcgtgatcg
     4261 cggcccgcgc ggcgacggcc gaggcgaccg aggccccagg cgcgacggcg aaggccgcgg
     4321 cgaccggggc ggcggccgcg accgcggccc gcgccgtgac ggcggcggcg gtggcggccg
     4381 aggcggcgac cgtgatcgcg gcccgcgcgg cgaaggcgg
//
LOCUS       DASWNU010000194         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_189539, whole genome shotgun sequence.
ACCESSION   DASWNU010000194 DASWNU010000000
VERSION     DASWNU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189539"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VGB57_03875"
     CDS             complement(<1..246)
                     /locus_tag="VGB57_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEY0130520.1"
                     /translation="MHSATIPLAAIATFAAVYWLAPAPAAVAQAEPGGIVETAAPVSA
                     RPVGPAPEAPEAPVYFAGCNEVRAAGLAPLFRGQPGYR"
     gene            520..789
                     /gene="infA"
                     /locus_tag="VGB57_03880"
     CDS             520..789
                     /gene="infA"
                     /locus_tag="VGB57_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0130521.1"
                     /translation="MAKEATLTVEGEIDEIYPDGRFGVMLDNQHRVIVYTAGKMRRFR
                     IRSVVGDRVHVEMTPYDLTKGRLIFRERTPGQGPGSSPRRPGAKR"
     gene            838..1065
                     /locus_tag="VGB57_03885"
     CDS             838..1065
                     /locus_tag="VGB57_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130522.1"
                     /translation="MGGTPDSTRTPIKPFLINKDEEGRVRLTLRETRYNGQGYPIVTT
                     TLHEEPFATATAARAYAKEHFGAEPGQFASK"
     gene            complement(1341..>3840)
                     /gene="ppdK"
                     /locus_tag="VGB57_03890"
     CDS             complement(1341..>3840)
                     /gene="ppdK"
                     /locus_tag="VGB57_03890"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY0130523.1"
                     /translation="FPDSLREEVAAGLAHIEGLCGKSFGDAADPLLVSVRSGARVSMP
                     GMMDTVLNLGLNDSTVQGLAAISGDPRFAWDSYRRFIQMYSDVVLGLDHGAFEEALEI
                     AKEDKGVHLDTELEAKDWERLTARYKALVEEQLGRPFPEDVHEQLWGAVGAVFGSWQS
                     DRAKTYRRLNSIPGDWGTAVNVQAMVFGNMGETSATGVAFTRNPSTGERAYYGEYLIN
                     AQGEDVVAGIRTPQYLTLAARQAAGAKAASMEESMPEVFGELARVFDLLERHYRDMQD
                     IEFTVERGRLFMLQTRSGKRTAKAALRIAVDMAEEGLITQEEAVLRVDPQALDQLLHP
                     TLDPAAERRVIAKGLPASPGAASGAAVFDADSAEKRAGLGEKVILVRVETSPEDIHGM
                     HAALGILTARGGMTSHAAVVARGMGRPCVSGAGTVSIDYKARTMRAGAIEVREGETIT
                     IDGSSGEVMLGAVATVQPELVGDFGTLMVWADAHRRLRVRTNAETPLDCRTAREFGAE
                     GIGLCRTEHMFFDAERIANVRQMILAADETGRRAALLKLLPAQRADFEEIFRIMAPLP
                     VTIRLLDPPLHEFLPHSEDEFAEVAAGAGVGVEALKRRVAELHEFNPMLGHRGCRLGI
                     TYPEIYEMQARAIFEAAVAVAREGGQAPIPEVMVPLVATRAELSIIKALVDRTAAAVF
                     EEQGASVGYLVGTMIELPRAALRAADIAGEAEFFSFGTNDLTQTTLGVSRDDAGRFLT
                     HYVEKGIFDRDPFVSIDEDGVGELIALAAERGRGARPGLKLGICGEHGGDPASIAFCE
                     KTGLDYVSASPYRVPIARLAAAQAALRARSSSRT"
BASE COUNT          616 a         1278 c         1343 g          603 t
ORIGIN      
        1 gcgataaccc ggctggcccc tgaaaagggg cgcgaggccc gcggcgcgca cttcgttgca
       61 gccggcgaaa tagaccggcg cctccggcgc ctccggcgcc gggccgaccg ggcgggccga
      121 cacgggcgcc gccgtctcga cgattccgcc aggctccgct tgcgccaccg cggcgggcgc
      181 cggggcgagc cagtagaccg cggcgaaggt ggcgatggcc gcgagcggga tggtggccga
      241 atgcatcgct cgacaatgag cggcccgggt ttcgaaaacg ctaagccggg ccgcggaatt
      301 tgaggaatca agttcgaagc cgacgaatcg ggattgcagg accgccgaag ttgctaaaat
      361 cgccccttgc gcctacatag gaagggctga cgtcgctgcg acggaatttg cggccctcgc
      421 ggccccgcct tcctttcatg cttctcgccc acgccaagcg atcgtcgccc gcgtggattt
      481 gcaatttacc ggccgcatgt tgccgaggga gaatcaggtt tggcgaagga agctacgctt
      541 accgtcgagg gcgagatcga cgaaatctat ccggacggcc gcttcggcgt gatgctggac
      601 aaccagcatc gggtgatcgt ctacacggcg ggcaagatgc gccgtttccg gatccgctcg
      661 gtcgtcggcg accgggtgca cgtcgagatg acgccctacg atctgaccaa gggccggctg
      721 atcttccgcg agcgcacccc cggccagggc ccgggcagca gcccgcggcg ccccggcgcg
      781 aaacgataat ccttcactcg acgttatcgt ccccaagaga gggacaggag actcattatg
      841 ggcggcaccc ccgacagcac ccgcaccccg atcaagccct tcctgatcaa caaggacgag
      901 gaaggacggg tccggctgac ccttcgcgag acccgctata acggccaggg ctatccgatc
      961 gtcaccacca ccctgcacga agagcccttc gccaccgcga ccgcggcgcg cgcttatgcc
     1021 aaggagcatt tcggcgccga gccggggcag ttcgcgagca agtaagccgt gggcggctga
     1081 ctattgccgg cgccatatcg cgaccgtcga cgcccgcacc ggcggccttt tgagttcgca
     1141 caaagaacac aaaggacacg aaggggcctc ggcggcgaag ccgcttcgtc tgttttcgcg
     1201 agcgagggaa tgattggtgc tgcgcactgt tcgcgccagc cccttagtgc tcttggtgtt
     1261 ctttgtgcga acccattcct cccggtggag cgggcacgag ccgcgcattt tcggcaccgg
     1321 cggtgttcat ggatcccgga tcaagtccgg gatgacgatc ttgccctgag cgcagcttgc
     1381 gccgccgcca gcctggcgat cggcacccgg tagggcgagg ccgagacgta atccaaaccc
     1441 gtcttctcgc aaaaggcgat cgaggccggg tcgccgccat gctcgccgca aatgccgagc
     1501 ttgaggccgg gacgggcgcc cctgccccgc tccgcggcca gcgcgatcag ctcgcctacg
     1561 ccgtcctcgt cgatcgagac gaacgggtcg cggtcgaaaa tgcccttctc gacatagtgg
     1621 gtcaggaagc gcccggcgtc gtcgcggctc actcccaagg tggtctgcgt gaggtcgttg
     1681 gtcccgaagc tgaagaactc cgcctctccg gcgatgtcgg cggcgcgaag cgcggcccgg
     1741 ggcagctcga tcatcgttcc gaccagatac ccgacgctcg cgccctgctc ttcaaacacg
     1801 gcggcggcgg tgcggtcgac cagcgccttg atgatgctaa gctcggcgcg ggtggcgacg
     1861 agcggcacca tcacttcggg aatgggcgcc tgcccccctt ccctcgccac cgcgacggcc
     1921 gcctcgaaga tggcccgggc ctgcatctcg taaatttcgg gataggtgat cccgagccgg
     1981 cagccgcgat ggccgagcat cgggttgaat tcgtgaagct cggcgacccg gcgcttcagc
     2041 gcttcgaccc cgaccccggc gccggccgcg acctccgcga actcgtcctc gctgtggggg
     2101 aggaattcgt ggagcggcgg gtcgagcagg cggatggtga cgggcagagg cgccatgatc
     2161 cggaagatct cctcgaaatc ggcgcgctgg gcggggagga gcttgaggag ggcggcgcgg
     2221 cggccggttt cgtcggcggc gaggatcatc tggcggacgt tggcgatgcg ctccgcatcg
     2281 aagaacatat gctcggtccg gcacaggccg atcccttcgg ctccgaactc gcgggcggtg
     2341 cggcagtcga gcggggtctc ggcattggtc cggacgcgga ggcggcggtg ggcgtcggcc
     2401 cagaccatca gggttccgaa gtcgccgacc agctcgggct ggacggtggc gacggcgccg
     2461 agcatgacct cccccgacga gccgtcgatg gtgatcgtct cgccttcgcg gacctcgatc
     2521 gcgccggcgc gcatcgtccg cgccttatag tcgatggaga cggtgccggc gccgctgacg
     2581 caggggcggc ccatgccccg ggcgacgacg gcggcgtggc tggtcatccc gccccgggcg
     2641 gtgaggatgc ccaaagcggc gtgcatcccg tgaatgtcct cggggctggt ctcgacccgg
     2701 acgaggatga ccttctcgcc gaggccggcg cgcttctcgg cgctgtcggc gtcgaacacg
     2761 gccgctccgg aggcggcgcc gggcgaggcg ggaaggccct tggcgatgac ccgccgctcc
     2821 gccgccggat cgagggtcgg gtggagcaac tggtcgagcg cctgcgggtc gacccggagc
     2881 accgcctcct cctgagtgat cagcccttcc tcggccatgt cgacggcgat ccgaagcgcc
     2941 gccttggcgg tgcgcttgcc cgaacgggtc tggagcatga agagcctgcc gcgctcgacg
     3001 gtgaactcga tgtcctgcat gtcgcgataa tggcgctcga gcaggtcgaa gacccgggcg
     3061 agctcaccga acacctcggg catggattct tccatcgacg ccgccttcgc cccggcggcc
     3121 tggcgggcgg cgagggtgag atattgcggc gttcggatgc cggcgacgac gtcctcgccc
     3181 tgggcgttga tcagatattc gccgtaataa gcgcgctcgc cggtcgacgg attgcgggtg
     3241 aaggcgactc cggtggcgga ggtctccccc atgttgccga acaccatcgc ctggacgttg
     3301 accgcggtgc cccagtcgcc ggggatcgag ttgaggcggc ggtaggtctt cgcccggtcg
     3361 gactgccagg agccgaacac cgctccaacc gcgccccaga gctgctcgtg gacgtcctcc
     3421 gggaagggcc ggccaagctg ctcctcgacc agcgccttgt agcgggcggt gaggcgttcc
     3481 cagtccttcg cctcgagctc cgtatcgagg tggacgccct tgtcctcctt ggcgatctcg
     3541 agcgcttcct cgaacgcacc atggtcgagg cccaggacga cgtcggaata catctggatg
     3601 aagcggcggt agctgtccca ggcgaagcgc ggatcgccgg agatcgcggc gaggccctgg
     3661 acggtggaat cgttgagtcc gaggttgagg acggtgtcca tcattccggg catcgacacc
     3721 cgggcgccgg agcggaccga gacgaggagc gggtccgcgg catcgccgaa gctcttgccg
     3781 cagaggcctt cgatatgggc gaggccggcg gcgacctcct cgcgcaggct gtcggggaag
//
LOCUS       DASWNU010000195         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_317766, whole genome shotgun sequence.
ACCESSION   DASWNU010000195 DASWNU010000000
VERSION     DASWNU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317766"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VGB57_03895"
     CDS             complement(<1..940)
                     /locus_tag="VGB57_03895"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY0130524.1"
                     /translation="MTTRTETAKADPPIESRDDLLAPFVKGEKPKEAWRIGTEHEKFV
                     YRRDDYRAPSYDEPGGIRDLLMALTEYGWKSVEEGGNVIALSGPDGTVSLEPAGQLEL
                     SGAPLEHLHQTCAEAGRHLNQVKAVGDKLGLGFLGLGMWPDKARADLPVMPKGRYAIM
                     LRHMPRVGTLGLDMMLRTCTIQVNLDYSSEADMAQKFRVGLALQPLATALFANSPFTE
                     GRPNGFLSFRSHLWSDTDPARTGMLPFVFEDGFGYERYLDYALDVPMYFVFRDGKYID
                     VAGESFRHFLDGKLPQLPGEKPRISDFIDHLSTAFPE"
     gene            complement(992..1747)
                     /locus_tag="VGB57_03900"
     CDS             complement(992..1747)
                     /locus_tag="VGB57_03900"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY0130525.1"
                     /translation="MPATPAWPPSSLPRLYVEDTLSQGAAVTLEGPQANYLSAVMRLG
                     EGAQVKLFDNRTGEWLAEIAEAGKKRVSLRILRHLREREPVPDLWLLFAPIKRGRIDW
                     LAEKATELGVARLVPVITRRTIVDRTKSDRLIAHMIEAAEQCERTALPELAEPEKLDA
                     LLGSWPEDRTLLFADEGGGEPMRAAMRPGPAAILIGPEGGFTDEERAAIRALAQAKPV
                     SLGPRILRADTAALAAISLWMAAAGDWDRAADR"
     gene            1775..>2182
                     /locus_tag="VGB57_03905"
     CDS             1775..>2182
                     /locus_tag="VGB57_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY0130526.1"
                     /translation="MTTESEIVPDSERRGLIGALPGPVRPYASLMRLDRPIGAWLLFW
                     PGAWAVALAGMDGRGVALILWLALGAWAMRSAGCVYNDIIDRKLDARVERTRLRPLAS
                     GRVSLQGAWSLLVGLSLIGLLVLLQLNLVAQIAS"
BASE COUNT          326 a          676 c          806 g          374 t
ORIGIN      
        1 cctccgggaa ggcggtcgac aaatggtcga tgaagtcgct gatgcgcggc ttttcgccgg
       61 gcaattgcgg gagcttgccg tcgaggaagt ggcgaaaact ttcgccggcg acgtcgatat
      121 atttgccgtc gcggaagacg aaatacatcg gcacgtcgag cgcgtagtcg aggtaacgct
      181 cgtagccgaa gccgtcttcg aacacgaagg gaagcatgcc ggtgcgggcg ggatcggtgt
      241 cggaccagag gtggctgcgg aaggagagga agccgttggg cctcccctcc gtaaacggcg
      301 aattggcgaa gagcgcggtc gccagcggct ggagggcgag gccgacgcgg aatttctggg
      361 ccatgtcggc ttcggacgaa taatcgaggt tcacctggat cgtgcaggtg cgaagcatca
      421 tgtcgaggcc gagggttccg acccgcggca tgtggcggag catgatcgcg tagcggccct
      481 tcggcatgac cggcaggtcg gcgcgcgcct tgtccggcca catgccgagg ccgaggaagc
      541 cgaggccgag cttgtcgccg accgccttca cctggttgag gtgacgcccc gcttcggcgc
      601 aggtctggtg gagatgctcc agcggcgcgc cggaaagctc gagctggccg gcgggttcga
      661 ggctgaccgt gccgtcgggg ccggagaggg cgatgacgtt gccgccttcc tccaccgact
      721 tccagccata ttcggtgagg gccatcagga ggtcgcgtat gccgcccggc tcgtcatagg
      781 agggggcgcg gtaatcatcg cgccggtaga cgaacttctc atgctcggtg ccgatccgcc
      841 acgcctcctt cggcttctcg cccttcacga acggcgcgag cagatcgtcg cggctttcga
      901 tcggcggatc ggccttggca gtttcggtgc gggtcgtcat ccgcgaccct ctagcggcgc
      961 gaggggaagc tcgccagcag gtttcaacac gctaccgatc ggcggctcga tcccaatcgc
     1021 cggcggccgc catccagagg ctgattgcgg caagcgccgc cgtgtcggcc cggagaattc
     1081 gagggccgag cgagaccggc tttgcttgcg ccaaggcgcg gatcgcggcg cgctcctcgt
     1141 cggtgaagcc gccttcgggc ccgatcagga ttgcggccgg gccgggtcgc atggccgcgc
     1201 gcatgggctc gcctccgccc tcatccgcga agaggagcgt gcggtcctcg ggccagctcc
     1261 cgagcagcgc atccagcttt tccggctccg ccagctcggg aagagccgtt cgttcgcatt
     1321 gctcggcggc ttcgatcatg tgcgcgatga gccggtcgga tttcgtgcgg tcgacgatgg
     1381 tgcgccgggt gatcaccggc acgaggcggg cgacgcccag ctccgtagcc ttttcggcga
     1441 gccaatcgat ccggccgcgt ttgatcggcg cgaagaggag ccagagatcg ggaacaggct
     1501 cgcgctcccg gaggtgccgc aggatgcgga gcgagacgcg cttcttgcct gcttcggcga
     1561 tctcggcgag ccattcgccg gtgcgattgt cgaacagctt gacctgtgcg ccctcgccca
     1621 gacgcatgac cgccgaaaga taattggcct ggggcccttc gagcgtcacc gcggcacctt
     1681 gggagagcgt gtcctcgaca tagagtcggg ggaggctgga cggcggccag gcgggggttg
     1741 cgggcatgct tcccactagg ccaaggaccg tgctgtgact accgagagcg aaatcgttcc
     1801 ggacagcgag cggcgggggc tgatcggtgc cctgccgggg ccggtccggc cctacgcttc
     1861 gctgatgcgg ctcgaccggc cgatcggcgc atggctgctc ttctggcccg gcgcctgggc
     1921 ggtcgccctg gcgggcatgg acgggagggg cgtagcgctg atcctctggc tagcgcttgg
     1981 cgcctgggcg atgcggagcg cgggctgcgt ctataacgac attatcgacc ggaagctcga
     2041 cgcgcgggtg gagcggacgc ggctgcggcc gctggcgagc gggcgggtgt cgctgcaggg
     2101 ggcttggtcg ctgctggtcg gcttgagcct gatcgggctt ctcgtcctgc tgcagctcaa
     2161 cctcgtggcg cagatcgcgt cg
//
LOCUS       DASWNU010000196         6375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_136508, whole genome shotgun sequence.
ACCESSION   DASWNU010000196 DASWNU010000000
VERSION     DASWNU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136508"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VGB57_03910"
     CDS             <1..434
                     /locus_tag="VGB57_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0130527.1"
                     /translation="VYETKETVKAKERPIVVITSNNEKELPDAFLRRCFFHYIKFPDR
                     ETMQAIVDVHFPGIQKILVQKAMDIFYEVREVPGLRKKPSTSELIDWLKLLLHEDMPL
                     DVLQSRDPSKAIPPLHGALLKNEQDVMLFERLAFMSRRPGG"
     gene            complement(550..1962)
                     /locus_tag="VGB57_03915"
     CDS             complement(550..1962)
                     /locus_tag="VGB57_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0130528.1"
                     /translation="MATRAIEEPDGDAINIVARNCGALAIECSDVSGYVSGVSDRISS
                     NLRTLDSLEEVTSRLLLDQARVAESTDEARILAEQARERLSEGRAAIEDTIGIFSGLT
                     DLVVQLGERMTGFAEAMSRVQNVSSSIERIAYRTNMLALNATIEAARAGEAGRSFAVV
                     AAEVKKLAQETRTATSEITGTIASLTREAEAVTSEIETGVGRSRVARSGFATITDTVR
                     QVAEIVALVDRQTDGIAQSTSHIQQSVDSVKSGLSSFAADARDNGGQLLQVHGRLTRL
                     ETLSNEMLDRLATSGVRTDDTPFIEFAQAGMREIRDVIEAAIVAREVGEGDVFDTRYT
                     AMPGTNPVQYETGFCDLADRRVRPVLDKLMAREPRLIACAITDNNGYLPTHISERSRP
                     QSDDPAWNAEHCRNRRNFLDDVTRRAIESDKEAMLATYSMDLGQGRYLPVKNVFVPLY
                     INGRRWGNFELAYRDEAMRL"
     gene            2154..3335
                     /locus_tag="VGB57_03920"
     CDS             2154..3335
                     /locus_tag="VGB57_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0130529.1"
                     /translation="MFFSFVDELRAAGITASMKEHLVLLEALEADIIRTSPEEFYYLA
                     RATFVKDEGLLDRFDQVFGKVFKGLEADYGTEAAPIPEEWLKKVAELYLSPEEMAKIQ
                     ALGSWEEIMEALKARLEEQQGRHQGGSKWIGTAGTSPFGAYGYNPEGIRIGQEGGRNK
                     SAIKVWDKREFRNLDNQIELGTRNIKVALRRLRRFAREGAADELDLDETIDGTARKGW
                     LDIRMRPERHNAVKLLLLLDVGGSMDPHVKVCEELFSAAHSEFKNLEFFYFHNCPYES
                     LWKDNRRRFAERTPTWDMLHKFGHDWKIVLVGDASMSPYEVTHPGGSVEHYNEEAGAV
                     WMQRLVDTYPSAVWLNPTPEKYWGYSQSTQIIRQLMHERMYALTLEGLDDAMRALSRK
                     V"
     gene            complement(3438..3899)
                     /gene="soxR"
                     /locus_tag="VGB57_03925"
     CDS             complement(3438..3899)
                     /gene="soxR"
                     /locus_tag="VGB57_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEY0130530.1"
                     /translation="MAYSTLLTIGELARRTGLSVSAIRFYETRGLVGAIRTGGNQRRF
                     MRSDIRRLSFALIAQRCGLTLGEIEKEMASLPNGRAPNRADWQSISERLRATLDERIA
                     MLERTRDQLDGCIGCGCLSLDTCALYNPEDRAARAGPGPRFLLGDRASDFA"
     gene            3989..4369
                     /locus_tag="VGB57_03930"
     CDS             3989..4369
                     /locus_tag="VGB57_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0130531.1"
                     /translation="MALPRIEHVNVTVSDPERAARLMESVFGWQVRWQGQAQKGGHTI
                     HVGTGDHYVALYTDPSRTYGGEDFAKGRPLNHIGVEVEELDETEARVIAAGLTPFSHG
                     DYEPGRRFYFLDPDGIEYEVVSYR"
     gene            complement(4616..4963)
                     /locus_tag="VGB57_03935"
     CDS             complement(4616..4963)
                     /locus_tag="VGB57_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0130532.1"
                     /translation="MRALIIEPQIFTSFMIEDALRDAGFTSIAMATTEEEAVACAEAE
                     PPDLVTAAVELERGSGIRAVERIRARLRVPVLFITQHIRQVRARDPKAPVVKKPFAAA
                     HLPPAIAAAKAIG"
     gene            complement(5183..5779)
                     /gene="recR"
                     /locus_tag="VGB57_03940"
     CDS             complement(5183..5779)
                     /gene="recR"
                     /locus_tag="VGB57_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY0130533.1"
                     /translation="MASPEIDALTQALARLPGLGPRSARRAVLHLMKKRDTALTPLLR
                     ALERVNDNLSICSACFNVDTNDPCSVCTDPRRDDRLLCVVEEVSDLWALDRSRLFPGR
                     FHVLGGRLSALEGVRPEDLNIDKLLGRVANGGIDEVVLAMNATLEGQTTAHYLAERLE
                     GFPVRLTQLAHGLPVGGELDYLDEGTLAQALRARRPVS"
     gene            5997..>6375
                     /locus_tag="VGB57_03945"
     CDS             5997..>6375
                     /locus_tag="VGB57_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY0130534.1"
                     /translation="MRIAFMGTPEFAVPTLDALVSAGHEVVAVYSQPPRPAGRGKALR
                     PSPVQQRAEALGIEVRTPLSLRDPEAQAAFAALDLDSAVVAAYGLILPPAILAAPRRG
                     CLNVHASRLPRWRGAAPIQRAILA"
BASE COUNT         1095 a         2038 c         2180 g         1062 t
ORIGIN      
        1 acgtctacga gaccaaggag acggtgaagg ccaaggagag gccgatcgtc gtcatcacgt
       61 ccaataacga gaaggagctt ccggacgcgt tcctgaggcg ctgcttcttc cattacatca
      121 aatttcccga ccgggagacg atgcaggcga tcgtcgacgt ccattttccg ggcatccaga
      181 agatcctcgt ccagaaggcg atggacattt tctacgaggt ccgcgaggtg ccggggctga
      241 ggaagaagcc ttcgaccagc gagctgatcg actggctgaa gctcctcctc cacgaggaca
      301 tgccgctcga cgtgctgcag tcccgcgacc cgtcgaaggc gatcccgccg ctgcacgggg
      361 ctttgctcaa gaacgagcag gacgtgatgc tgttcgagcg gctggccttc atgagccggc
      421 ggccgggggg ctagaggcgg cgaccgtcac cccggcgaaa gccggggcct caggacgaga
      481 agtctcggtg ggtctctccg aggtcccggc tttcgccggg atgaggggat attccttccc
      541 gtcccgccgt cagagccgca tcgcctcgtc gcggtaggcg agctcgaaat tgccccagcg
      601 gcgaccgttt atgtagagcg gaacgaagac gttcttgacc ggcagatagc ggccctggcc
      661 gaggtccatg gagtaggtgg cgagcatcgc ctccttgtcg ctttcgatcg cgcggcgggt
      721 gacgtcgtcg aggaagttgc ggcggttgcg gcaatgctcg gcgttccagg cggggtcgtc
      781 gctctgcggc cgcgagcgct cggaaatgtg ggtgggcaga tagccgttat tgtcggtgat
      841 cgcacaggcg atcagccgcg gctcgcgggc catcaacttg tcgaggacgg gccggacgcg
      901 gcggtcggcg agatcgcaga agccggtctc gtactggaca ggattggtgc cgggcatggc
      961 ggtgtagcgg gtgtcgaaga cgtcgccctc gccgacttcg cgggcgacga tcgcggcttc
     1021 gatcacgtcc ctgatctcgc gcatccccgc ctgcgcgaac tcgatgaacg gcgtgtcgtc
     1081 ggttcggact ccggaggtgg ccaggcggtc gagcatctcg ttggagaggg tctcgagcct
     1141 ggtcaggcgg ccatggacct ggagcaattg cccgccattg tcgcgcgcgt cggcggcgaa
     1201 gctcgacagc cccgatttga cggagtcgac gctctgctgg atgtggcttg tcgattgggc
     1261 gattccgtcg gtctggcggt cgaccagagc aacgatctcg gcgacctggc ggaccgtgtc
     1321 ggtgatggtg gcgaagccgc tccgggcgac ccggcttcgg ccgaccccgg tctcgatctc
     1381 ggaggtcacc gcttcggcct cgcgggtcag cgaggcgatg gtgccggtga tctcgctggt
     1441 cgcggttctc gtctcctggg ccagcttctt cacctcggcg gcgacgacgg cgaagctccg
     1501 ccccgcctcc cccgcccgcg ccgcctcgat ggtggcgttc aaggcgagca tgttggtccg
     1561 gtaggcgatc cgctcaatgc tggaggagac gttctggacc cggctcatcg cctcggcgaa
     1621 tccggtcatg cgctcgccca actggacgac gaggtcggtc aaaccgctga agatcccgat
     1681 cgtgtcctcg atcgcagcgc ggccttccga caatcgctcg cgcgcctgct cggcgaggat
     1741 ccgcgcctcg tcggtcgatt ccgccacccg ggcctggtcg agcagaagcc ggctggtcac
     1801 ctcctcgagg gaatcgaggg ttcgaaggtt ggacgaaatc cgatcggaga ctccggagac
     1861 atagccggag acgtcgctgc attctatcgc cagcgccccg caattgcgcg cgacgatgtt
     1921 gatcgcatcg ccgtccggtt cctcgatcgc cctcgttgcc acgctttgcc ccctgtcggt
     1981 ggttcgggag tgagggctaa gggagaaaga ttgaaggctc gttaaccact ggtccttccc
     2041 gtcatcccgg cgaaagccgg gacctcagga cgataagtca cggtgggtct ccccgagacc
     2101 ccggccttcg ccggggtgac gcaccgcgcg taacgagtta cacgcggcgc gtcatgttct
     2161 tctccttcgt cgacgagctt cgcgccgcgg gcataaccgc ctcgatgaag gagcacctcg
     2221 tgcttctgga ggcgctggag gccgacatca tccgcaccag cccggaagaa ttctactatc
     2281 tcgcccgggc caccttcgtg aaagacgaag gcctgctcga ccgcttcgac caggtgttcg
     2341 gaaaggtctt caagggcctc gaggcggatt acggaaccga ggcggcgccg atccccgagg
     2401 agtggctgaa gaaggtcgcc gagctctacc tgagcccgga ggagatggcg aagatccagg
     2461 cgctgggctc ctgggaagag atcatggagg cgctcaaggc acggctcgag gagcagcagg
     2521 gacgccacca gggcggctcg aaatggatcg gcaccgccgg cacctccccc ttcggcgcct
     2581 acggctacaa tcccgaagga atccggatcg gccaggaagg cggccgcaac aagagcgcga
     2641 tcaaggtctg ggacaaacgg gagttccgca acctcgataa ccagatcgag ctcggcaccc
     2701 gcaacatcaa ggtcgcgctc cggcgcctcc gccgcttcgc ccgcgaaggc gccgccgacg
     2761 agctcgatct cgacgagacg atcgacggca cggcgcgcaa gggctggctc gacatccgaa
     2821 tgcgccctga gcgacacaat gcggtgaagt tgctgctcct gctcgacgtc ggcggctcga
     2881 tggatccgca cgtcaaggtc tgcgaggagc tcttctcggc ggcccattcg gagttcaaga
     2941 atctggaatt cttctacttc cacaattgcc cctacgagag cctgtggaag gacaatcgcc
     3001 gccgcttcgc cgagcgcact ccgacctggg acatgctcca taaattcggc cacgattgga
     3061 agatcgtgct tgtcggcgac gcttcgatga gcccctacga ggtcactcat cccggcggct
     3121 ccgtcgagca ttataacgag gaagccggag cggtctggat gcagcgcctg gtcgacacct
     3181 atccgagcgc ggtgtggctg aacccgacgc cggaaaaata ttggggctac agccagtcca
     3241 cccagatcat ccgccagctg atgcacgagc gcatgtacgc gctgacgctc gaaggcctgg
     3301 acgacgcgat gcgggcgctg agccggaagg tttaggaccg ggaaggatat tcgtcatccc
     3361 ggcgaaagcc gggacctcgg ggagacccgc agagacttct cgtcccgggg tcccggcttt
     3421 cgccgggatg acgggggtca ggcgaagtcg gacgccctgt cccccagcaa aaaccgaggc
     3481 cccggcccgg cgcgggcggc gcggtcttcc ggattgtaga gcgcgcaggt gtcgagcgac
     3541 aggcagccgc agccgatgca gccgtcgagc tggtcccggg tccgctcgag catcgcgatc
     3601 cgctcgtcga gggtcgcgcg aagacgttcg ctgatcgact gccagtcggc ccgattggga
     3661 gctcggccgt tcggcagcga cgccatctcc ttctcgatct cgccgagggt gagcccgcag
     3721 cgctgggcga tgagggcgaa ggacaagcgg cggatgtcgg agcgcatgaa gcggcgctga
     3781 ttgccgccgg tgcggatcgc gccgaccagg ccgcgggtct cgtagaagcg gatcgccgag
     3841 accgaaaggc cggtccggcg ggccagctcg ccgatggtga ggagggtcga gtatgccatg
     3901 aaaaaacctt cccgaagaag cttgacctca acttagcttg aggttgtacg actcgcaaat
     3961 cgaaggggcc ggagcgagga gaacaggaat ggcattgccc aggatcgagc atgtgaacgt
     4021 gacggtgagc gaccccgaga gggccgccag gctgatggaa tcggtgttcg gctggcaggt
     4081 ccgctggcag ggacaggcgc agaaaggcgg gcacacgatc cacgtcggca ccggcgacca
     4141 ttatgtcgcg ctctataccg acccgagcag gacctatggc ggcgaggatt tcgccaaggg
     4201 ccggccgctc aaccatatcg gagtcgaggt cgaggagctg gacgagactg aggcgcgagt
     4261 gatcgcggcc ggcctcaccc ctttctccca cggcgattac gagccggggc ggcgcttcta
     4321 cttcctcgac cctgacggaa tcgaatatga ggtggtgagc taccgctaac cttcgcctcg
     4381 ccgtcactct gaactcgttt cagtgtccag ctcgtcgtcc gccccggacg gtcggatctg
     4441 ggtctaaccc cgacccggcc cccggtgccg aggcgccaat tcacggagaa ggcctcgaaa
     4501 gaaatccagg gcctgccggc cggcgaggaa acactcgatg caggggcgtc ggacggctga
     4561 gcggttcgac cctggacgct gaaacaagtt cagcatgacg gactgttcgc gggcgtcagc
     4621 cgatcgcctt cgccgcggcg atggcgggcg ggaggtgggc ggcggcgaag ggcttcttca
     4681 cgaccggggc cttcgggtcg cgcgccctca cctgccggat atgctgggtg atgaacagca
     4741 ccggcaccct gagtcttgct cggatcctct cgacggcgcg aatcccgctg ccgcgctcga
     4801 gctcgacggc ggcggtgacg aggtccggcg gctcggcctc ggcgcaggcc acggcttcct
     4861 cctccgtcgt cgccatcgcg atcgacgtga agccggcatc cctcagggcg tcctcgatca
     4921 tgaaggacgt aaaaatctgt ggctcgatga tcagagcgcg cattattttc tcttgtcttg
     4981 aactcggtgg ccgcggggac cgtgaacaac ccgacgaaca gcctcaacga acctgatcta
     5041 tgttggttgc ctcaatccca gaaaaaaagg cttttgtttc ccgtctgtta tgactcgtct
     5101 ccagcggagg ctgggccccg tggggtgaag gccgcagcac ctttcacccg ctgggggccc
     5161 agccttcgct gggacgacgg aattaggaaa ccggccggcg tgcccggagc gcctgggcaa
     5221 gggtgccctc gtcgagatag tcgagctccc cgccgaccgg caggccgtgg gcgagctggg
     5281 tgaggcggac ggggaagcct tcgaggcgct cggcgagata atgggcggtg gtctggcctt
     5341 cgagggtcgc gttcatcgcc agcacgacct cgtcgatgcc cccgttggcg acccggccga
     5401 gcaatttgtc gatgttgaga tcctcgggcc ggaccccctc cagcgccgaa agccggccgc
     5461 cgaggacgtg gaagcggccc gggaagaggc gcgagcggtc gagcgcccag aggtcggaca
     5521 cttcttccac cacgcagagc aggcggtcgt cgcgccgggg atcggtgcag acgctgcagg
     5581 ggtcgttggt gtcgacgttg aagcaggccg agcagatcga gagattgtcg ttcacccgct
     5641 ccagggccct cagcagcgga gtcaaagccg tgtcgcgctt cttcatcagg tgaagcacgg
     5701 ccctgcgcgc cgagcgcggc ccgagcccgg ggagccgggc gagcgcctgg gtgagcgcgt
     5761 cgatttcggg agaagccatg aggggtaggt agggagtcgt tcgggtgggc gcaaatcctc
     5821 tgcctccctg ccgcaaagcg gtggggaggg ggaccatgcg aagcatggtg gaggggtaac
     5881 tgcccgggaa tccgaggttc gagagcgaga tggcggaagc ctgcgccaat tacccctcca
     5941 ccacacttcg tgtggtcccc ctccccatct ggagatgggg aggctaggga cgcaccatgc
     6001 gcatcgcttt catgggaacg cccgaattcg ccgttccgac gctcgacgcc ctcgtttcgg
     6061 cggggcacga ggtcgtcgcc gtctactcgc agccgccgcg tccggcgggg cgcggcaagg
     6121 cgcttcgccc ctcccccgtc cagcagcgcg ccgaggcgct ggggatcgag gtccggaccc
     6181 cgctctcgct cagggacccc gaggcccagg ccgctttcgc cgcgctcgac ctcgattccg
     6241 ccgtggtcgc agcctacggg ctgatcctgc cgcccgcgat cctcgccgcg ccgcgccggg
     6301 gctgcctcaa cgtccacgcc tcgcgccttc cgcgctggcg cggggcggcg ccgatccagc
     6361 gcgccatcct cgccg
//
LOCUS       DASWNU010000197         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_162344, whole genome shotgun sequence.
ACCESSION   DASWNU010000197 DASWNU010000000
VERSION     DASWNU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162344"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..457
                     /locus_tag="VGB57_03950"
     CDS             218..457
                     /locus_tag="VGB57_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130535.1"
                     /translation="MSFKMIFASTAALAMVAGTPAFAAKSQNQNQQVLSSEAGGAGVK
                     AKKICRSFPHSASRMKRERLCLTRADWKKFEADQM"
     gene            complement(582..1136)
                     /gene="dcd"
                     /locus_tag="VGB57_03955"
     CDS             complement(582..1136)
                     /gene="dcd"
                     /locus_tag="VGB57_03955"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY0130536.1"
                     /translation="MSIMSDKWIRAQALEHGMIEPFVDGQKREGTISYGLSSYGYDAR
                     VSDEFKLFTNVDNAIVDPKDFKANSFVDRKTDVCIIPPNSFALARTVEYFRVPRDVLV
                     ICLGKSTYARCGIIVNVTPLEPGWEGHVTLEFSNTTPLPAKVYANEGACQFLFLQGNE
                     PCEVSYADRAGKYMGQRGVTLPKL"
     gene            complement(1244..1708)
                     /locus_tag="VGB57_03960"
     CDS             complement(1244..1708)
                     /locus_tag="VGB57_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEY0130537.1"
                     /translation="MAEPRIIDIELDERTIIRRSDDIERERKVAIFDLLESNSFQPVG
                     HEGPFRIMLRIEDNSLAIDLRNEAGEPLETIRLGLTRFKRPVRDYFAICETYFKTVRS
                     DSPKGLETIDMARRGLHNEAAELLRECLAGKIEMDFDTARRLFTLICVLHIK"
BASE COUNT          320 a          610 c          632 g          346 t
ORIGIN      
        1 cccggcgaaa gccgggacct cggcgacacc cgccgagcct tttctccacg aggtcccggc
       61 tttcgccggg atgacgcagg gcagccgaat ccccctcccg cgcctccctt ttcgaccaac
      121 cgccgccatg cttctgccga cgacccgcgc gcctatgggt ttcgccgcgg gcaccgctta
      181 ctcttgtgga agggatggag acacgaggag aaagtggatg agtttcaaga tgattttcgc
      241 gtcgaccgcc gcgctcgcga tggtcgccgg gacgccggcc ttcgcggcca agagccagaa
      301 ccagaaccag caggtcctct cgtccgaggc cggcggcgcc ggcgtgaagg cgaagaagat
      361 ttgccgctcc ttcccccaca gcgcctcccg catgaaacgg gagaggctct gcctgacccg
      421 cgcggactgg aagaagttcg aggcggatca gatgtaaggc agtgccctcc tctgccgtca
      481 tgccggactt gatccggcat ccatgaacac gggctagtga aatctatcgc ccctccgtgt
      541 tcatggattc cggctttcgc cggaatgacg gccaagggcc gctacagctt gggcagcgtc
      601 accccccgct ggcccatata tttgccggcg cggtcggcat agctgacctc gcagggctcg
      661 ttgccctgaa ggaacaggaa ctggcaggcg ccttcattgg cgtagacctt ggcggggaga
      721 ggcgtcgtgt tcgagaattc gagagtgaca tggccttccc agccgggctc gagcggggtc
      781 acgttgacga tgatcccgca gcgcgcatag gtcgatttgc cgaggcagat gacgagcacg
      841 tcgcgcggca cccggaaata ttcgaccgtt ctcgccagag cgaagctgtt cggcgggatg
      901 atgcagacgt cggtcttgcg gtcgacgaag ctgttcgcct tgaagtcctt cgggtcgacg
      961 atcgcgttgt cgacgttggt gaacaatttg aactcgtcgg agacccgggc gtcgtagccg
     1021 tagctcgaca ggccgtagct gatcgtccct tcccgcttct ggccgtcgac gaacggctcg
     1081 atcatgccgt gctccagcgc ctgggcgcgg atccacttgt cggacatgat gctcatgcgt
     1141 ttactccgtc atcccggcgc aagccgggac ctcgggtcga gaagtctcgg cgggtctccc
     1201 ccgaggttcc ggctttcgcc ggaatgacgg ggaggagcaa gcctcacttg atgtggagga
     1261 cgcagatcag ggtgaagagg cggcgggcgg tgtcgaagtc catctcgatc ttgccggcca
     1321 ggcactcgcg gagaagctcg gccgcctcgt tgtggaggcc gcgccgggcc atgtcgatcg
     1381 tctccagccc cttgggcgaa tcggagcgga cggttttgaa ataggtctcg cagatggcga
     1441 aatagtcgcg gaccggccgc ttgaagcggg tgaggccgag gcggatcgtc tccagcggct
     1501 cgcccgcctc gttcctcagg tcgatggcca ggctgttgtc ctcgatcctc agcatgatcc
     1561 ggaacgggcc ctcatgcccc accggttgga agctgttcga ttcgagcagg tcgaagatgg
     1621 cgaccttgcg ctcgcgctcg atatcgtcgc tccggcggat gatcgtgcgc tcgtcgagct
     1681 cgatgtcgat gatccgcggc tcggccatgc gagggcgtgt agacggggcg gggcggcgtg
     1741 tcatcgtcat cccggcgaag gccgggacct cggcccccgt catcccggcg aaggccggga
     1801 cctcggcccc cgtcatcccg gcgaaagccg ggacctcggg gagacccgcc tcctccgtca
     1861 tcccggcgaa ggccgggatc catgaacacg gcttgacgaa acctggca
//
LOCUS       DASWNU010000198         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_136740, whole genome shotgun sequence.
ACCESSION   DASWNU010000198 DASWNU010000000
VERSION     DASWNU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136740"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="VGB57_03965"
     CDS             complement(<1..1611)
                     /locus_tag="VGB57_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEY0130538.1"
                     /translation="MADAGILNDRGKDLSQPLDRLSPDERLKKSSNYLRGTIATGLLD
                     RITGAVATDDTRLMKIHGMYQQDDRDLRDERRRQKLEPAYRFMIRVRLPGGVCTPEQW
                     LKLDALARAHGGEQLRLTTRQTFQFHWVLKEEVRAAIQGLHEALLDTVAACGDVSRNV
                     MCAADPEDSALHAEVVEAARTVSDEYLPKTRAYHEIWYGEEKVASSDEEEPFYGATYL
                     PRKFKIGFVVPPSNDIDVYTQDLGFIAIKGPDGLEGYNVAIGGGMGRTENEPLTYPRL
                     ADVIGYIPKDRLVAATGAVMSVQRDYGNRAERNRARFKYTIDDKGLGWIKAEIEARLG
                     APLESAKAFHFTSNGDSFGWRETGSGRSNCTLYVESGRLANRPGRAWLDGLRAVAQVH
                     RGTFRVTPNQNLIVAGIASEDRPAIEALLAEHGLADRSGSATRLNSIACVALPTCGLA
                     MAEAERYLPGILARIEAILSFHGLLDEPITFRISGCPNGCSRPYVAEIALTGRGPGKY
                     NLYLGGGFHGERLNRLYRENIGEAAILEI"
     gene            complement(1613..>2103)
                     /locus_tag="VGB57_03970"
     CDS             complement(1613..>2103)
                     /locus_tag="VGB57_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase [NADPH] flavoprotein
                     alpha-component"
                     /protein_id="HEY0130539.1"
                     /translation="VYIQSNEQFRLPEDDKAIIMIGAGTGIAPYRAFLQEREARGSSG
                     KSWLFFGERNFRSDFLYQTEWQAWLKDGLLGRMDVAFSRDQGGKAYVQHRMVERAGDL
                     FAWLEDGAHLYLCGDAARMAPDVHETLISIVAEEGRLQREGAEEYVRELQRSRRYQRD
                     VY"
BASE COUNT          328 a          670 c          736 g          369 t
ORIGIN      
        1 gatttccagg atcgcggcct cgccaatatt ctcccggtag agccggttca gccgctcgcc
       61 gtggaagccg ccgcccagat agagattgta cttgcccggc ccccggccgg tcagtgcaat
      121 ttcggcgaca taaggtcgcg aacagccatt ggggcagccg ctgatccgga aagtgatcgg
      181 ctcgtcaagc agcccgtgaa aggagaggat ggcctcaatc cgagcaagga tgccggggag
      241 gtagcgctcg gcctccgcca ttgcgaggcc gcaggtcggg agcgcgacgc aggcaatcga
      301 gttgagccgg gtcgcgcttc ccgagcggtc ggcaaggccg tgctcggcga gcagcgcctc
      361 gatcgcgggc cggtcctcgg atgcaatgcc ggcaacgatc agattctggt tgggggtgac
      421 cctgaaggtg ccgcgatgca cctgcgcgac cgcccgaagg ccgtcgagcc aggcccggcc
      481 cggccggttg gcgaggcggc cgctttcgac atagagggtg cagttggagc ggccgctccc
      541 ggtctcgcgc cagccgaagg aatcgccgtt cgaggtgaag tggaaggcct tggccgattc
      601 gagcggagcg ccgaggcggg cctcgatctc ggccttgatc cagcccagcc ccttgtcgtc
      661 gatcgtatat ttgaaccggg cgcggttgcg ctcggcgcgg ttgccgtagt cgcgctggac
      721 cgacatcacc gccccggtcg cggcgaccag ccggtccttg gggatatagc cgatgacgtc
      781 ggcgagccgg ggataggtga ggggctcgtt ctccgtgcgg cccatgccgc cgccgatggc
      841 cacgttatag ccttcgagcc cgtccggacc cttgatggcg atgaaaccga gatcctgggt
      901 gtaaacgtcg atgtcattgg acggcgggac gacgaagccg atcttgaact tccgcggcag
      961 ataggtggcg ccgtagaagg gttcctcctc gtcggacgag gcgaccttct cctcgccgta
     1021 ccagatttcg tgataggcgc gggtcttcgg cagatattcg tcgctgaccg tccgggccgc
     1081 ttcgacgact tcggcgtgga gcgccgaatc ctcgggatcg gcggcgcaca tgacgttgcg
     1141 cgagacgtcc ccgcaagcgg cgacggtgtc gagcagggct tcgtgaaggc cctggatggc
     1201 ggctcgcacc tcctccttca gaacccagtg gaactggaag gtctgtcggg tggtaaggcg
     1261 cagctgctca ccgccatggg cccgcgccag ggcgtcgagc ttcagccatt gctccggagt
     1321 gcagactccg ccggggagcc tgacccggat catgaagcga taggccggct cgagcttctg
     1381 gcgccgcctc tcgtcgcgca ggtcgcggtc gtcctgctgg tacatgccgt ggatcttcat
     1441 cagccgggtg tcgtcggtgg cgacggcgcc ggtgatgcgg tcgagaaggc cggtggcgat
     1501 cgttccacgc agatagttgc tggacttctt cagccgctcg tccgggctca gccggtcgag
     1561 cggctgcgag aggtccttgc cgcggtcgtt taggattcct gcgtcggcca tgtcagtaga
     1621 cgtcccgctg ataacggcgg ctgcgctgca gctcgcggac atattcctcg gcgccctcgc
     1681 gctggaggcg cccctcctcg gcgacgatcg agatcagggt ctcgtgaacg tccggcgcca
     1741 tccgcgccgc gtcgccgcac agatagagat gggcgccgtc ctccagccag gcgaagagat
     1801 cgccggcgcg ctcgaccatc cggtgctgga cataggcctt gcccccctgg tcgcgcgaga
     1861 aggcgacgtc catccggccg agcaggccgt ccttcagcca ggcctgccat tcggtctggt
     1921 agagaaagtc gctgcggaag ttgcgctcgc cgaagaacag ccaggatttg ccgctcgagc
     1981 cccgggcctc gcgctcctgg aggaaggcgc gatagggggc gatgccggtg ccggctccga
     2041 tcatgatgat cgccttgtcg tcctccggca gccggaactg ctcgttcgac tggatgtaga
     2101 cgg
//
LOCUS       DASWNU010000199         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_475705, whole genome shotgun sequence.
ACCESSION   DASWNU010000199 DASWNU010000000
VERSION     DASWNU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475705"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGB57_03975"
     CDS             <1..471
                     /locus_tag="VGB57_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY0130540.1"
                     /translation="YAFDGSGLREAETIPAWLRPLRARAAALAGHQPEAFVHALVIEY
                     APAAGIGWHRDRPVFGDVVGISLLAPAPLRFRRKSGTKWERFTLTAEPRSAYLLRGEA
                     RHEWEHSIPPLETLRYSVTLRTLIASPSKMGRGSARRWWRGRRRRPERLLGGSI"
     gene            complement(541..1203)
                     /locus_tag="VGB57_03980"
     CDS             complement(541..1203)
                     /locus_tag="VGB57_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY0130541.1"
                     /translation="MNRIVRAAGFAAAVFALVAGASYSDPASAGETATKAPAVVSDQA
                     APASTMTDPSLFSKMWANVTSLVTPKAETPAPAAISPAKPLAELVLAYAGTQTGDREM
                     ECLANAVYFEARSEPIEGQLAVAEVVMNRAASGRYPTDLCSVITQKAQFSFINRGRFP
                     RADRGSEAWKKAVAIASIARQKLAGNLPSSVLWYHATYVSPSWGKRLTRQTQIGLHIF
                     YS"
BASE COUNT          260 a          523 c          551 g          274 t
ORIGIN      
        1 tatgcgttcg acggcagcgg gctcagggaa gcggagacga taccggcctg gctccggccg
       61 ttgcgcgccc gagccgccgc tttggccggg catcagccgg aggctttcgt ccatgccctg
      121 gtgatcgaat atgccccggc cgccggaatc ggctggcacc gcgaccggcc ggtcttcggc
      181 gacgtcgttg gaatctcgct tctcgccccg gcgccgctcc gcttccgccg caaatcgggg
      241 acgaagtggg agcgtttcac cctcaccgcc gagccccgct cggcctatct cctgcgcggg
      301 gaggcgaggc acgaatggga gcacagcatc cccccgctgg agaccctgcg ctattcggtg
      361 acgctgagga ccttgattgc ctccccatcg aagatgggga gggggtccgc ccgaaggtgg
      421 tggaggggta gaaggcgcag gccggagagg cttctcgggg gctccattta gacctcgcca
      481 tgtacccctc caccaggctt cgcctggtcc ccctcccccc gcttcgcgcg gggaggcaaa
      541 tcaagaatag aagatatgca gcccgatctg ggtctgcctg gtgaggcgct tgccccagct
      601 cggggagacg taggtggcgt gataccagag cacgctggag ggcaggttgc cggccagctt
      661 ctggcgggcg atgctggcga tggcgacggc cttcttccag gcttcggaac cgcggtcggc
      721 gcgcgggaat cggccgcggt tgatgaagga gaattgcgcc ttctgggtga tgacggagca
      781 caaatcggtc gggtagcggc ccgaggcggc ccggttcatc accacttcgg cgacggcgag
      841 ctggccttcg atcggctcgc ttcgggcctc gaaataaacg gcgttggcga ggcattccat
      901 ctcgcggtcg ccggtctggg ttccggcata agcgaggacg agctcggcga gcggtttcgc
      961 cggcgagatc gccgccgggg ccggggtttc ggccttcgga gtcaccagcg aggtgacgtt
     1021 ggcccacatc ttggagaaaa ggctgggatc ggtcatggtc gacgccggag cggcctggtc
     1081 cgaaacgacg gccggagcct tggtggcggt ttcgccggcg gaggcggggt cggagtagct
     1141 ggcccctgcg acaagcgcaa agacagccgc ggcgaagccc gcggcgcgaa cgatccggtt
     1201 catctagtct ctttttacat gcggccggct gatcccccgg atgcgagaac cgcatcccct
     1261 gatcgccccc cgtctcgagc tcccgccggc cgctgatcgg ctgcggcgga cccgctctcg
     1321 ctactaagct gaggggcaga tggtcgaagt cggttcgccg cgcaacccgc cggcggcggc
     1381 gaagcgatga gtcgtgtgac tcaagggaca cgtcgaaaat aaattcgact tatccgggaa
     1441 ccttccaaaa aatccgcatt tttcaagcac ctggaagggc ggaattcgcc gcctccgaaa
     1501 cgtttaccgc ctctgcgggc ttctccccgc ccgattgctc gcccgcgatc accttgacga
     1561 aggcgctctg cacccagccc gaccggcacg gcccgtcata gtcgcggc
//
LOCUS       DASWNU010000200         3943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_344884, whole genome shotgun sequence.
ACCESSION   DASWNU010000200 DASWNU010000000
VERSION     DASWNU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344884"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VGB57_03985"
     CDS             complement(<1..573)
                     /locus_tag="VGB57_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0130542.1"
                     /translation="MPATHSTRMLILGSGPAGLSAAIYGARAGLAPIVVQGIQPGGQL
                     TTTTDVENYPGFRDVVQGPWLMEEMQAQAEHVGARMMWDHIVEVDLSRRPFRLVGDGG
                     TIYTGDVLVIATGAQAKWLNLPSEEHLKGKGVSACATCDGFFYRGKKVAVIGGGNTAV
                     EEAIYMTNHSHDVTLIHRRDSLRAEKILQDR"
     gene            669..1721
                     /gene="hppD"
                     /locus_tag="VGB57_03990"
     CDS             669..1721
                     /gene="hppD"
                     /locus_tag="VGB57_03990"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY0130543.1"
                     /translation="MPHENPLGLDGFEFVEFTSPDPEALGDLFNRLGFAHRHDHKTKR
                     VRHYAQGDINFLLNMEASGHADDFRGAHGPSASAMAFRVADAERALAMAVERGATRVE
                     GEYGLPAIEGIGGSYLYLVDRYGAGTIYDRDFVPAQGAEATASSGLHTLDHLTHNVHR
                     GRMDHWAQFYERIFNFRQIRYFDIEGQQTALLSRAMTAPDDKIRIPLNESQDEFSQIE
                     EFLKEYKGEGIQHLALATDDIFATVDQLRANGVTFQDSPETYYDMIDARVPGHGHSIA
                     EMRKRRILIDGSPETGEGLLLQIFTENMIGPIFFEIIQRKGNEGFGEGNFKALFESIE
                     LDQIRRGVIPAASEHA"
     gene            complement(1814..2602)
                     /locus_tag="VGB57_03995"
     CDS             complement(1814..2602)
                     /locus_tag="VGB57_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0130544.1"
                     /translation="MPEPEVRIGTAGWTIPASAAEAFPGEGSGLERYSARFDCAEINS
                     SFHRSHRPDTWRRWAASVPDGFLFSAKLSREITHEKRLADCAAPLAAALAEMEGLGER
                     LAILLVQLPPSLAFDRQVAEAFLTALRCLWDRDVAFEPRHPSWFEPEAEALLADRRIA
                     RVAADPAKVPAAAEPGGWGGLVYYRLHGSPIVYRSSYDDGRLEAYAARLAAATVPTWC
                     IFDNTASSAATGDALKLQTLFASLPRSGGEGDHPKGGGGVEGSA"
     gene            complement(2624..2998)
                     /locus_tag="VGB57_04000"
     CDS             complement(2624..2998)
                     /locus_tag="VGB57_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130545.1"
                     /translation="MGFGTRLAEERKRLGLKQAEFASLVGTDVPKQSLYENDRRELRA
                     DYLSRLAAAKVDVVYVLTAKRSEGAWLGEEASQLLSAYLALPEDLQEAVLRLVADLNR
                     HFALGSRTLHSDRQDYRSEEPD"
     gene            complement(3261..>3943)
                     /locus_tag="VGB57_04005"
     CDS             complement(3261..>3943)
                     /locus_tag="VGB57_04005"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130546.1"
                     /translation="DGMFLRGNYTIDFNAPGYTGLFTNIENLTLTSATDERYARGGGT
                     EFDYNLVLSDAIVGAGQVLTVSGAILTASETMAFDASAETNGVLRLFGGKASDTLKGG
                     GQNDLIHGNLGADTLAGNNGADAFRYQAIFESDAANMDQIVDFTPGTDRIELDRIDAD
                     TLSAGNQAFNWIGSSAFTGSASQLRAYEQNGTWFVEGDVNGDGSADLVIALTLQGPNP
                     LGAGDFLL"
BASE COUNT          683 a         1301 c         1300 g          659 t
ORIGIN      
        1 acggtcctgg aggatcttct cggcacgaag gctgtcgcgg cgatggatca gagtgacgtc
       61 gtggctgtga ttggtcatgt agatagcttc ctcgaccgcg gtgtttccgc cgccgatgac
      121 cgccaccttc ttgccgcggt agaagaagcc gtcgcaggtc gcgcaggccg agacgccctt
      181 gcccttcaga tgctcctcgc tcggcaggtt cagccatttg gcctgggcgc cggtggcgat
      241 gacgaggacg tcgccggtgt agatcgtgcc gccgtcgccg acgaggcgga acgggcggcg
      301 gctgagatcg acctcgacga tatggtccca catcatccgg gcgccgacat gctcggcctg
      361 ggcctgcatc tcctccatca gccacggccc ctggacgacg tcgcggaagc ccggataatt
      421 ctcgacgtcc gtggttgtgg tgagctggcc gccgggctgg atgccctgga cgacgatcgg
      481 cgccaggccg gcgcgggcgc cgtagatcgc tgcggagagc ccggccgggc ctgagccgag
      541 gatgagcatg cgggtggaat gggttgcggg catgggggcg agatagggtc tgcgaccgcc
      601 cccgcgcaac ccatttgccg ctgccccggc ggcttggtag agggcgcgcg atcctcgagg
      661 agttgaagat gccccacgaa aacccgctcg gcctcgacgg cttcgagttc gtcgaattca
      721 ccagccccga tccggaggct ctcggcgacc tgttcaatcg cctcggcttc gcccaccgcc
      781 acgaccacaa gaccaagcgg gtgcgccatt atgcgcaggg cgacatcaac ttcctgctca
      841 acatggaggc ctcgggccac gccgacgact tcaggggcgc ccacggcccc tcggcgagcg
      901 ccatggcctt ccgggtcgcc gatgccgagc gggcgctggc gatggcggtc gagcgggggg
      961 cgacgcgagt cgagggcgaa tatggcctgc ccgcgatcga ggggatcggc ggctcctacc
     1021 tctatctggt cgaccgctac ggcgccggga ccatctatga ccgggatttc gtccccgccc
     1081 agggcgcgga ggcgacggct tcgtcgggtc tgcacacgct cgaccacctc acccacaatg
     1141 tccaccgcgg ccggatggat cattgggcgc aattctacga gcggatcttc aacttcaggc
     1201 agatccgcta cttcgatatc gagggccagc agaccgccct cctctcccgg gcgatgaccg
     1261 cgccggacga caagatccgt atcccgctca acgagagcca ggacgaattc agccagatcg
     1321 aggagttcct gaaggaatat aaaggcgagg gaatccagca tctcgctttg gccaccgacg
     1381 acatcttcgc caccgtcgac cagctgcgcg ccaacggcgt caccttccag gacagcccgg
     1441 agacctatta cgacatgatc gacgcccggg tccccggcca cggccactcg atcgccgaga
     1501 tgcgcaagcg ccggatcctg atcgacggct ctcccgagac gggcgaaggc ctgctcctcc
     1561 agatcttcac cgagaacatg atcggcccga tcttcttcga gatcatccag cgcaagggca
     1621 acgagggctt cggcgagggc aatttcaagg ccctgttcga atcgatcgag ctcgaccaga
     1681 tccgccgcgg ggtcatcccg gccgccagcg agcacgccta attgcctccc catgctcagc
     1741 atggggaggg ggaccatgtg caacatggtg gagggtaact ggcgatgaag ctgacgattc
     1801 gcaccgaaac ctctcaggcc gatccttcta cccctccacc acccttcggg tggtccccct
     1861 ccccaccgct tcgcggcagg gaggcaaaga gtgtttgcag cttcagcgcg tcgcccgttg
     1921 ccgccgagga ggcggtattg tcgaagatgc accaggtcgg aactgtggcc gcggccagac
     1981 gcgccgcata ggcctcgagc cggccatcgt catagctcga tcgatagacg atgggggagc
     2041 cgtgcagccg gtaataaacg agccctcccc agccgccggg ctcggccgcg gccggaacct
     2101 tcgccggatc ggccgcgact cgggcgattc ggcggtccgc cagcagcgcc tcggcctccg
     2161 gctcgaacca gctcgggtgg cgcggctcga aggcgacgtc gcggtcccag aggcaccgga
     2221 gcgcggtcag gaaggcttcc gcgacttgcc gatcgaaggc taggctaggc ggcagctgga
     2281 cgaggaggat tgcgagccgc tcgcccagcc cctccatctc ggccagcgcc gcggcaagcg
     2341 gcgcggcgca gtccgcgagc cgcttttcat gggtgatctc cctggaaagc ttggcggaga
     2401 agagaaagcc gtccggcacg gacgcagccc agcgccgcca ggtgtcgggg cggtgcgagc
     2461 ggtggaagga ggaattgatc tcggcgcaat cgaagcgggc cgaatatcgt tcgaggccgc
     2521 tgccctcgcc ggggaaggcc tcggcggccg aagccgggat cgtccagccg gcggtgccga
     2581 ttcgcacttc gggttcgggc atccgccgcg aacgcacgga cgcctagtcc ggctcctcgg
     2641 agcgatagtc ctggcggtcc gaatggaggg tacggcttcc cagtgcgaaa tggcggttga
     2701 ggtcggcgac gagccgaagc acggcttcct gcaaatcctc gggcaaagcg agataggcgg
     2761 agagaagctg gctggcctct tccccgagcc aggcaccctc gctcctcttg gcggtgagca
     2821 cgtagaccac gtccaccttg gccgcggcga gccgcgacag atagtcggcg cgcagttccc
     2881 gccggtcgtt ctcatagagg ctctgcttgg gaacgtcggt tccaacaagc gaagcgaact
     2941 cggcctgctt gaggccgagc cgcttccttt cctccgcaag cctagtgccg aaacccatcg
     3001 aaactgacga tccccaatcg actggccctc gcaaaacgac ggcctaccac gaaattaatc
     3061 cgaatcggtt cattgcacag ccgatttcaa tctgtctcct gcattttttg gcattggacg
     3121 tttgcggggg atcttggcgt ccaggcggac agccccgatc catgatttcc gggccgggcc
     3181 ggggaaggtt gaaccggggc cgaagcaagt cctcgcccca cccgtgccca gtgttccggc
     3241 cttcgagcaa tgacgggaaa ctagagcagg aagtcccccg cgccgagggg attcgggccc
     3301 tggagggtga gggctatgac gaggtcggcg ctgccgtcgc cgttcacgtc gccctcgacg
     3361 aaccaggtcc cgttctgctc ataggcgcgg agctgactgg ccgagccggt gaacgcgctc
     3421 gagccgatcc agttgaaggc ctggttgccc gcgctcaggg tgtcggcgtc gattcggtcg
     3481 agctcgatcc tgtcggtgcc gggggtgaaa tccacaatct ggtccatatt ggcggcatcg
     3541 gactcgaaaa tcgcttggta gcggaacgca tcggccccgt tattgcccgc cagggtgtcg
     3601 gcgccgaggt tgccgtggat gaggtcgttc tggccgccgc ccttcagagt gtcggaggcc
     3661 ttgccgccga acagccgaag aacgccgttc gtctccgcgc tcgcgtcgaa cgccatcgtc
     3721 tcgctcgccg tcaggatggc gcccgacacc gtcagcacct gccccgctcc cacgatcgcg
     3781 tcggacagga cgaggttgta gtcgaactcc gtgcccccgc ccctcgcata gcgctcgtcg
     3841 gtggcggacg tgagggtcaa attctcgatg ttggtgaaca ggccggtgta cccgggagcg
     3901 ttgaagtcga tcgtatagtt gcccctcagg aacatgccgt cat
//
LOCUS       DASWNU010000201         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_8337,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000201 DASWNU010000000
VERSION     DASWNU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8337"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VGB57_04010"
     CDS             <1..1131
                     /locus_tag="VGB57_04010"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130547.1"
                     /translation="GGAGNDNIFFGATLTQFDVVTGGADTDTLVLQGNYSGGLTLSAK
                     VTEIENVSLLGGDNTSLGEPGTNRYDYALTTNDSNFAAGVQARINGAALLAGEDFTFN
                     GSAETDGLFVVYGGRGRDTLTGGVGNDIFFFAEERFASGDVVNGGAGYNGMFLRGNYT
                     IDFNAPGYTGLFTNIHNLTLTSATDERYARGGGTEFDYNLILSDAIVGSGQQLTVSGA
                     ILTASETMVLDASAETDGTLRLFGGKASDTLKGGALNDLIHGNLGADTLAGNGGVDAF
                     RYQNISESSSGSMDRILDFTPGTDKIELDRIDANALVAGNQAFSWIGSSAFTGSAGQL
                     RAYEQGGSWFVEGDVNGDGSADLVIALTLQGPTPLGAGDFLL"
     gene            1161..4427
                     /locus_tag="VGB57_04015"
     CDS             1161..4427
                     /locus_tag="VGB57_04015"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0130548.1"
                     /translation="MANTIHALGAARPRDVAISKDGATVYASFADGYVRAYDAGSGAL
                     VGEWDVGTTLGAMDLSPDGTFLIVAEAEPLTSYYAPDTSDNQFTVATYRLALGSGEVT
                     TYLHTARGYEYAFFDVAVLSNNKVLVTERALPGWSATLYPLELDLGTGQYEVVEDRRV
                     TTNTWLSPSEDGRWVFMAEGNSSDPKLDMYRTPLGFAAWRHNYQDDVTGYNDGFQAYS
                     GEAGLAVQVVGRRILILDNALRLQRDLTQTYTQWQDYGIYGAAFNAGGTRLFLLDNEA
                     QSIAILSTSDWSLLGTIPIDGLPVISGRGDYGNRLMVAPGENYITVVTDSGLYAVANP
                     NPTNVVLTGTGAAEVLTGTPFDDSIAALGGNDWVLGGLGADSIDGGDGDDVLAGGADG
                     DLVHGGSGRDLLYGGDGNDRLDGGGGDDELQGGRGFDRFDGGSDNMAADPASYGDRIS
                     FYDRTATRGANADLRTGTVWDDGFGNQQETFTGIESFGGGTAFADTFIGGDGRNSIVL
                     ARGDYGHGFGGPDLFVLEAAPAQIYGGDEMSDWIELRSGGMWLIPDGDGDGLADLAPA
                     MTSGWTIDLLNHSITDGYGNTGSVIMVPNVRGSVFDDMITGDLWKNILDGGAGADRMT
                     GGVHDDIYYVDNAGDVVVELDDEESAMEGRADEVRTALASYVLAANVEKLTATSDSAH
                     DFRGNGDDNVVTGGGGADLLRLHDGGDDTVLAGAGNDNIFFIGSLTAADVVNGGEGTD
                     TLVLQGPYGSLTLTANVTQIENISILGGNNTVFGEPGTNRHDYVLTTVDSNFAAGIQA
                     RINGAALLAGEDFTFDGSAETDALFVVYGGKGRDTLTGGQGNDIFFYAEERFASGDTV
                     NGGAGYDGMFLRGNYTIDFNAPGYTGLFTNIENLTLTSATDERYARGGGTEFDYNLTL
                     SDAIVNPGYQLTVSGAVLTASETMVFDASAETDGTLRLFGGRASDTLKGGALNDLIHG
                     NLGADTLAGNGGADAFRYQNVAESSSGSMDHILDFTPGTDKIELDRVDANALVAGNQA
                     FSWIGSSAFTGSAGQLRAYEQGGTWFVEGDVNGDGGADLVIALTLQGPTPLGAGDFLL
                     "
BASE COUNT          803 a         1540 c         1560 g          728 t
ORIGIN      
        1 ggcggagcgg gcaacgacaa tatcttcttc ggcgcgaccc tcacccagtt cgacgtcgtg
       61 acgggcggag cggataccga cacgctcgtc ctccagggca attattccgg cggactgacc
      121 ctgtcggcca aggtcaccga gatcgagaac gtttcgctcc tcggcggcga caacaccagc
      181 ctcggcgagc cgggcaccaa ccgctacgat tatgcgctga ccaccaacga ttcgaacttc
      241 gccgcgggcg ttcaggcccg gatcaacggc gccgccctgc tcgccggcga ggacttcacc
      301 ttcaacggct cggcggagac ggacgggctc ttcgtcgtct acggcggcag gggcagggac
      361 accctgaccg gcggcgttgg caacgacatc ttcttcttcg cggaggaaag gttcgcgagc
      421 ggggacgtgg tgaacggcgg cgcgggctat aacggcatgt tccttcgcgg caattacacg
      481 atcgacttca acgctcccgg ctataccggc ctgttcacca acatccacaa tctcaccctc
      541 acgtcggcga cggacgagcg ttatgcgagg gggggcggca ccgagttcga ctacaacctc
      601 atcctctccg acgcgatcgt cgggagcgga cagcagttga cggtcagcgg cgccatcctg
      661 acggcgagcg agacgatggt gctcgacgcc agcgccgaga cggacggcac cttgcggctg
      721 ttcggcggca aggcctcgga cacgttgaag ggcggagctc tgaacgacct catccacggc
      781 aacctcggcg ccgacactct cgcgggcaat ggcggcgtgg acgccttccg ctaccagaac
      841 atctcggagt cgagctcggg atcgatggac cggatcctcg acttcacccc ggggacggac
      901 aagatcgagc tcgaccgcat cgacgccaac gcgctcgtcg cgggcaacca ggcgttcagc
      961 tggatcggct cgagcgcgtt caccggctcg gcggggcagc ttcgagccta tgagcagggc
     1021 ggctcctggt tcgtcgaggg cgacgtgaac ggcgacggca gcgccgacct cgtcatcgcc
     1081 cttacgctgc aggggccgac tccgctcggg gctggcgact tcctgcttta ggcgtcatac
     1141 cttccaatcg gggaaattcg atggcgaaca cgatccatgc cctgggcgcc gctcggccgc
     1201 gcgatgtggc gatcagcaag gacggggcga ccgtctacgc ctcgttcgcc gacggctatg
     1261 tgcgggccta cgatgcgggc agcggcgcac tggtcggcga gtgggacgtc ggcaccaccc
     1321 tgggcgccat ggacctgtcg ccggacggca cgttcctgat cgtcgccgag gccgagccgc
     1381 tcacctctta ttacgcgccg gacacttcgg acaatcagtt caccgtcgcc acctacaggc
     1441 tggcgctcgg cagcggcgag gtgacgacct atctccacac cgcccgcggc tacgaatatg
     1501 cctttttcga cgttgccgtg ctttcgaaca acaaggtgct ggtgaccgag cgagccctgc
     1561 ccggctggtc cgccacttta taccccctcg agctggacct ggggacgggc caatatgaag
     1621 tggtcgaaga caggagggtc accacgaata cctggctttc gccgtcggag gacggccgct
     1681 gggtcttcat ggccgagggc aacagctccg acccgaagct cgacatgtac cggaccccgt
     1741 tgggcttcgc tgcgtggcgc cacaattatc aggacgatgt caccggctac aatgacggct
     1801 tccaggccta tagcggcgag gccgggctcg cggtccaggt ggtcgggcgc cggatcctca
     1861 tcctcgacaa tgcgcttcgg ctccagcgcg atctcaccca gacctatacg cagtggcagg
     1921 actacggcat ctacggcgcc gccttcaacg ccggcggaac gcgcctcttc ctcctcgaca
     1981 atgaagcgca atccatcgcc atcctgtcga ccagcgactg gagcctgctc gggacgatcc
     2041 cgatcgacgg cctgccggtc atttcgggac gcggcgacta tggcaaccgg ctgatggtcg
     2101 cgcccggcga gaattacatc acggtggtca ccgacagcgg cctctacgcg gtggccaatc
     2161 ccaatccgac gaacgtcgtc ctcaccggca ccggcgcggc cgaagtgttg accggcactc
     2221 cgttcgacga ctcgatcgcc gctcttggcg gaaacgattg ggtgctcggc gggctcggcg
     2281 ccgacagcat cgacggcggc gacggcgacg acgtcctggc cggcggggcg gacggggatc
     2341 tcgtccacgg cggttccggc cgcgacctcc tctatggcgg agacggcaac gaccggctcg
     2401 atggcggcgg cggcgacgac gagctgcagg gcggccgcgg gttcgaccgg ttcgacggcg
     2461 gctcggacaa tatggccgcc gaccccgcca gctatggcga ccggatcagc ttctacgacc
     2521 ggacggcgac ccggggggcc aatgccgacc ttcgcaccgg caccgtctgg gacgacggct
     2581 tcggcaacca gcaggagacc ttcaccggga tcgaatcctt cggcggaggc accgccttcg
     2641 cggacacttt catcggcggc gacggacgca actcgatcgt cctcgcccgg ggcgattatg
     2701 gccacgggtt cggcggcccc gacctttttg tgctcgaagc ggcgcccgcc caaatctacg
     2761 gcggcgacga aatgagcgac tggatcgagc ttcgttcagg cggaatgtgg ctgattcccg
     2821 acggcgacgg cgacggcctt gccgatctgg ccccggcgat gacctcgggc tggacgatcg
     2881 acctcctcaa ccattcgatc accgacggct acggcaatac gggctcggtc ataatggtcc
     2941 cgaacgtgcg cggcagcgtc ttcgacgaca tgatcaccgg cgacctgtgg aagaacatcc
     3001 tcgacggcgg cgccggcgcg gacaggatga ccggcggagt gcatgacgac atttattatg
     3061 tcgacaatgc aggggacgtc gtcgtcgagc tggacgacga ggagtccgcc atggagggca
     3121 gggcggacga ggtgcggacg gcgctggcga gctacgtcct cgccgccaat gtcgagaagc
     3181 tgaccgcgac gtcggattct gcccacgatt tccgcggcaa tggcgacgac aatgtcgtca
     3241 ccggcggcgg cggcgccgac ctgctgcgcc tccacgacgg cggcgacgac acggttctgg
     3301 ccggcgccgg caacgacaac atcttcttca tcgggtcgct gacggcggcg gatgtggtga
     3361 acggcggcga ggggacggat acgctcgtgc tgcagggccc gtacgggtcg ctgacgctga
     3421 cggccaacgt cacccagatc gagaatatct cgatcctcgg cggcaacaac accgttttcg
     3481 gcgagcccgg caccaaccgc cacgactacg tccttaccac ggtggattcg aacttcgcgg
     3541 ccggcattca ggcgcggatc aacggcgcgg cgttgctggc gggcgaggac ttcaccttcg
     3601 acggctcggc ggagacggat gcgctgttcg tcgtctacgg cggcaagggc agggacaccc
     3661 tgaccggcgg gcagggcaac gacatcttct tctacgccga ggagcggttc gcgagcgggg
     3721 acacggtcaa cggcggcgcc ggctatgacg gcatgttcct gaggggcaat tacacgatcg
     3781 acttcaacgc tccgggctac accggcctgt tcaccaacat cgagaacctc accctgacct
     3841 cggcgacgga cgagcgctat gcgaggggcg ggggcacgga gttcgactac aacctcaccc
     3901 tttccgacgc gatcgtgaac ccggggtacc agctgacggt gtcgggcgcg gtgctgacgg
     3961 cgagcgagac gatggtgttc gacgccagcg cggagacgga cggcaccttg cggctgttcg
     4021 gcggcagggc ctcggatacc ttgaagggcg gggcactgaa cgacctcatc catggcaacc
     4081 tcggcgccga cactttggcg ggcaatggcg gggcggacgc cttccgctac cagaatgtcg
     4141 cggagtcgag ctcggggtcg atggaccata tcctcgactt cacgccgggg acggacaaga
     4201 tcgagctcga ccgcgtcgac gccaacgcgc tcgtcgcggg caaccaggcg ttcagctgga
     4261 tcggctcgag cgccttcacc ggctcggcgg ggcagcttcg agcctatgag cagggcggca
     4321 cctggttcgt cgagggcgac gtgaacggcg acggcggcgc cgacctcgtc atcgccctga
     4381 cgttgcaggg gccgacgccg ctcggagcgg gggacttcct gctctgacgg ggtggccagc
     4441 gggtcttccg gaaggctaga ccatgacctg acgggcttcg cggcggccga ccgagcggta
     4501 gatgcgcggc ccgaggtcga ggcggaacgg gcccatcttc tcggtcggga cctccgcctc
     4561 gcgcccgacc agccaggagc gcgaggttcg gcccgcgccg cctcccgccc agtcgtaggc
     4621 gacgttgatc g
//
LOCUS       DASWNU010000202         4740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_475770, whole genome shotgun sequence.
ACCESSION   DASWNU010000202 DASWNU010000000
VERSION     DASWNU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475770"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1224
                     /locus_tag="VGB57_04020"
     CDS             322..1224
                     /locus_tag="VGB57_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0130549.1"
                     /translation="MRRLPPLTSIEAFVQVARLGSVKAAAEALALSSPALSRRIQALE
                     RFVGHPLFERRHQAVHLNPDGERLMAEIGPAIDALALAMERATGSVEMMRLRLAVPSL
                     FASQRLMPKLPGLRALHPDLHIDIDTGSNRVARLDEGLDAAIAITTEVDPSLYSRRID
                     SNCVIAIGSSALQAGPNAIREPAQLAGATIFLHRDMPDSFAYWRDSIGLPDLQPAAID
                     HFDSGQLILDAAAQGLGVAFMLESHLYGSGDDRLVRLFDHKVESPYSYWFACRRSALK
                     RRPVRIFHDWLFDRLLKEEMREAA"
     gene            complement(1365..2120)
                     /locus_tag="VGB57_04025"
     CDS             complement(1365..2120)
                     /locus_tag="VGB57_04025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0130550.1"
                     /translation="MARRVTLVTGASAGLGVDFARQCRKRGDELVLVARRRDRLEALA
                     AELGFAHVIPADLAEPGAPARLLAEVAGLGLEVDTLINNAGFGAAGAFVDSPPERLLE
                     MIDLNVRALTELCRLALPPMLERRRGFILNVASTAAFQAGPYSAVYYATKAYVLSLSE
                     ALHEESKKSGVHVSALCPGPTATEFFEVAGSPNGRLAKMATDPKAVVAAGLAGLARNK
                     AIVVPGTMNKMTSQAGRILPRAALRRAVAALKG"
     gene            complement(2123..2584)
                     /locus_tag="VGB57_04030"
     CDS             complement(2123..2584)
                     /locus_tag="VGB57_04030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0130551.1"
                     /translation="MADELNTLVLNLSSGGDVVIRLRPDLAPGHVARIRELVEEGFYD
                     GVVFHRVIPGFMAQGGDPTGSGMGGSDRPDLKAEFSREPHVRGVASMARSQNPNSANS
                     QFFICFDDARFLDNQYTVWGQVTEGMEHVDALPKGEPPREPGRIVKASLRP"
     gene            2829..4244
                     /gene="mgtE"
                     /locus_tag="VGB57_04035"
     CDS             2829..4244
                     /gene="mgtE"
                     /locus_tag="VGB57_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY0130552.1"
                     /translation="MSESEIDLLGAPPGSPPEGRLDEDDRLKPGYVSEVMERVDAGDF
                     DGARALVEPLHPADLADLFELVDSDDRRALAAAIADLLDGDVLAEMNEHVREELIDAL
                     DPRQVADLAGEMETDDAVAMIEDLEEDEQRAVLRELDPDDRAAIEEALTYPEESAGRL
                     MQRDLIAVPEHWTVGQVIDYLRAGDDLTTDFWEVFVVDERHHPVGTCMLSWILRTHRH
                     VAVADVMKREQTLIPVDMDQEEVALRFQKYALISAAVVDQSGRLVGMITVDDIVHIIQ
                     EEASEDVLLLSGAGEGDINQPLLESYSSRVRWLIANLGTALVAATIIGLFEGTISKMV
                     ALAALMPIVAGVGGNAGTQTLAVTVRALATNQLTQSNTWRAILREIQVACLNGVTVAA
                     VIGVGVGLFFANPALGAVIAAAMLVNIIIAGFAGVLVPLALERAGADPAVASSVFVTM
                     VTDSMGFLVFLGLATASGVAG"
     gene            4381..>4740
                     /locus_tag="VGB57_04040"
     CDS             4381..>4740
                     /locus_tag="VGB57_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HEY0130553.1"
                     /translation="MPLNISKVAVGCASLETLEKRQRLRLAEGIVPVVTRFRPKRAEE
                     LIGGSIYWIVKHRIAARQTIVGFADREEDRRTIIRLDPRLVPVRALAKRAHQGWRYLE
                     AQDAPLDLDGTEDGVDIL"
BASE COUNT          742 a         1626 c         1591 g          781 t
ORIGIN      
        1 tcctcgccgg gctcgccacc ggagtgctga tcgccctgct gttctggctg atcgagcgtc
       61 ggcgctgacg cgtgcggccg tcgccctgaa gcatcgcacc ctggatgagg acaaaaaaag
      121 ggccggtccc gaaggaccga ccctgaaagt tttttaggag aggatgcctg aaaggcaggt
      181 tctttctctg cttcggccga caattgtgca agtgcgaaca agtgtgctac acatgcaatt
      241 gttgcaacct gatttgctga gtttcgcgct tgttattacg gcggcggctc ttaggtgcag
      301 cgcacaagag gagacgcctc catgcgccga ctgcctccgc tgacgtcgat cgaggccttc
      361 gtccaggtcg cccggctggg ctcggtcaag gccgccgccg aagctctggc cctgtcctcg
      421 ccggccttgt cgcggcgaat ccaggcgctc gagcgcttcg tcggccatcc cctgttcgag
      481 cggcgccacc aggcggtcca cctcaatccg gacggcgagc ggctgatggc ggagatcggg
      541 ccggcgatcg acgccctcgc tctggcgatg gagcgggcga cgggcagcgt cgaaatgatg
      601 cggctgaggc tcgccgtacc ctcgctcttc gcctcgcagc ggctgatgcc gaagctgccc
      661 ggccttcgcg ccctccatcc cgatctccat atcgacatcg acaccggctc caaccgggtg
      721 gcccgcctcg acgaggggct cgacgccgcc atcgccatca ccaccgaggt agatccctcg
      781 ctttattcgc ggcggatcga cagcaattgc gtcatcgcca tcggctcgag cgcccttcag
      841 gcgggtccga acgcgataag ggagccggcc cagctcgccg gagccaccat cttcctccac
      901 cgcgacatgc ccgacagctt cgcctactgg cgcgactcga tcggactgcc cgatctgcag
      961 ccggcggcga tcgaccattt cgattcgggc cagctgatcc tcgacgccgc cgcccagggg
     1021 ctcggcgtcg ccttcatgct cgaaagccac ctctacggct ccggcgacga caggctggtc
     1081 cggctgttcg accacaaggt ggagagtccc tacagctact ggttcgcctg ccgccgctcc
     1141 gcgctgaagc gccgcccggt ccggattttc cacgactggc tgttcgaccg gctgctgaag
     1201 gaagagatga gggaagcggc ttgattttgc ctccctgccg cgaagcggtg gggaggggga
     1261 ccacccgaag ggtggtggag gggtagctgg cagaagcctg ggcattaccc ctccaccacg
     1321 cttcgcatgg tccccctccc catgcttcgc atggggaggc aagattagcc cttcaaagcc
     1381 gccacggccc gtctcagcgc ggctctcggc aggatccgtc cggcctgcga ggtcatcttg
     1441 ttcatcgtcc cgggaacgac gatcgccttg ttgcgggcga ggccggcgag gccggcggcg
     1501 acgaccgctt tcgggtcggt cgccatcttc gcgagccggc cgttcgggct tcccgcgact
     1561 tcgaagaact ccgttgcggt cgggccgggg cagagcgccg agacatggac tccgctcttc
     1621 ttcgactcct cgtgaagagc ctcgctgagc gacaggacat aggccttggt cgcgtaatag
     1681 acggcgctgt aggggccggc ctgaaaggcg gcggtcgaag cgacgttgag gatgaagccg
     1741 cggcggcgct ccagcatcgg cggcagcgcc agccggcaga gctcggtcag cgcgcggacg
     1801 ttgaggtcga tcatctcgag cagacgctcg ggcggcgaat cgacgaaggc cccggcggcg
     1861 ccgaagccgg cattgttgat cagggtgtcg acctcgaggc cgaggcccgc cacctcggcg
     1921 agcaggcgcg ccggggcgcc cggctcggcg aggtcggcgg ggatgacgtg ggcgaagccg
     1981 agctccgccg ccagcgcctc cagcctgtcc ctgcgccggg cgaccaggac cagctcgtcg
     2041 ccgcgcttgc ggcattgccg ggcgaaatcg acgccgagcc cggccgaagc gccggtgacg
     2101 agcgtgaccc gccgcgccac cgtcagggcc tgagggaggc tttgacgatc cggcccggct
     2161 cgcgcggcgg ctcgcccttg ggcaaagcgt cgacatgctc catcccttcg gtgacctggc
     2221 cccagacggt gtactggttg tcgaggaagc gggcgtcgtc gaagcagatg aagaactggc
     2281 tgttggcgct gttcggattc tgcgagcgcg ccatcgacgc gaccccgcgg acgtgcggct
     2341 cgcggctgaa ctcggccttg aggtcgggcc ggtcggagcc gcccatgccg ctcccggtcg
     2401 ggtcgccgcc ctgcgccatg aagccgggga tgacccggtg gaagacgact ccgtcgtaaa
     2461 agccctcctc gaccagctcc cggatccggg cgacgtgccc gggcgcgaga tcgggccgaa
     2521 gcctgatgac gacgtcgccg ccgctgctga ggttgaggac cagggtgttg agttcgtcgg
     2581 ccatgatttt ctccgtgcta tgcgtcgtcc cggcgaaggc cgggacccat gaacaccgtc
     2641 attgtcgaac aggtgaccag cgctcgtctc cgtcgacccg atccgtgttc atggatcccg
     2701 gccttcgccg ggatgacggg tgagtgggac ataagcgccc ggctcccacc gcgccacccc
     2761 gctcgacact cccgccgcct cgtcctagga agcgcctcgt cgaagatcct gggcgaaagg
     2821 aggggcggat gagcgagagc gaaatcgatc tgcttggcgc tccgcccggc tcgccgcccg
     2881 agggccgcct cgacgaggac gaccggctca agcccggcta tgtcagcgag gtgatggagc
     2941 gggtcgacgc gggcgacttc gacggcgccc gcgcgctggt cgagccgctc catcccgccg
     3001 acctcgccga cctgttcgag ctggtcgaca gcgacgaccg ccgcgcgctc gccgccgcga
     3061 tcgccgacct gctcgacggc gacgtgctcg ccgagatgaa cgagcatgtc cgcgaggagc
     3121 tgatcgacgc gctcgacccg cgccaggtcg ccgacctcgc cggcgagatg gagacggacg
     3181 acgccgtcgc catgatcgag gatctcgagg aggacgagca gcgcgccgtc cttcgcgagc
     3241 tcgatcccga cgaccgcgcc gcgatcgagg aggcgctcac ctatcccgag gaatcggccg
     3301 gccggctgat gcagcgcgat ctgatcgcgg tgcccgagca ttggacggtc ggccaggtca
     3361 tcgactatct gcgtgccgga gacgatctga ccaccgattt ctgggaagtg ttcgtcgtcg
     3421 acgagcgcca ccatccggtc ggcacctgca tgctgagctg gattctccgg acccatcgcc
     3481 acgtcgccgt cgccgacgtg atgaagcgcg agcagaccct gatcccggtc gacatggacc
     3541 aggaggaagt ggcgcttcgc ttccagaaat acgcgctcat ctcggcggcc gtcgtcgacc
     3601 agtcgggccg gctggtggga atgatcacgg tcgacgacat cgtccacatc atccaggagg
     3661 aagcgtcgga ggacgtcctc ctcctctcgg gcgccggcga gggcgacatc aaccagccgc
     3721 tcctcgaaag ctattcgagc cgggtgcgct ggctgatcgc caatctcgga accgcgctgg
     3781 tcgctgccac gatcatcggc ctgttcgagg gcacgatctc gaagatggtc gctctcgccg
     3841 ccttgatgcc gatcgtcgcc ggagtcggcg gcaatgcggg cacccagacc ctggcggtca
     3901 cggtccgggc gctcgcgacc aaccagctga cccagtccaa tacctggcgc gcgatccttc
     3961 gcgagatcca ggtcgcctgc ctcaacggag tcacagtcgc cgcggtgatc ggagtcggag
     4021 tgggcctctt cttcgccaat ccagccttgg gcgcggtgat cgcggcggcg atgctcgtca
     4081 acatcatcat cgccggcttt gccggagtcc tcgtgccgct cgccctcgag cgggcgggcg
     4141 ccgacccggc ggtggcctcg tcggtgttcg tgacgatggt gaccgattcg atgggcttcc
     4201 tggtcttcct cggtctcgcc accgcctccg gcgtcgccgg ctgagcgaat cgtcaccccg
     4261 gcgaaagccg gggcctcgtg gagacccgcc gtgccttttc cgtcccgggt cccggctttc
     4321 gccgggatga cggggatatt cctattgccc caaccactta tcgaattaag acgcggggcc
     4381 atgccgctca acatcagcaa ggtcgccgtc ggctgcgcca gcctggagac gctcgagaag
     4441 cgccagcggc ttcggcttgc cgagggaatc gtgcccgtgg tcacccgctt ccgcccgaag
     4501 cgggcggaag agctgatcgg cggctcgatc tactggatcg tcaagcacag gatcgcggcc
     4561 cgacagacca tcgtcggctt cgccgaccgc gaggaggatc gccggacgat catccgtctc
     4621 gatccgcggc tggtgccggt ccgggccctt gcgaaacgcg cgcatcaggg ctggcgctac
     4681 ctcgaggcac aggacgcgcc gctcgatctc gacggcacgg aggacggggt ggacatcctt
//
LOCUS       DASWNU010000203         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_345024, whole genome shotgun sequence.
ACCESSION   DASWNU010000203 DASWNU010000000
VERSION     DASWNU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345024"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGB57_04045"
     CDS             <1..193
                     /locus_tag="VGB57_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130554.1"
                     /translation="PISSQTISLVTAPFYPDRRRGRSPNPSPPGEAPDRTPPPPPPPA
                     ALTLVRPRRNLAGQPRDPP"
     gene            190..561
                     /locus_tag="VGB57_04050"
     CDS             190..561
                     /locus_tag="VGB57_04050"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEY0130555.1"
                     /translation="MIHAAATLLLSAVQAPDAPSTAAPWRDLGLSRGYHLFYDPAPVA
                     RNGETLTARVLTRFPGAAASEPAYVIGTVEIRCGAGEARVTRTTNHLADGSVAREDTA
                     PMPFAAIRPATLFATLRQALC"
     gene            761..2140
                     /locus_tag="VGB57_04055"
     CDS             761..2140
                     /locus_tag="VGB57_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130556.1"
                     /translation="MEQSRNPESRSGRDWREDWDGQDASIPDPPWEPDAIDLLLHPEL
                     AQGAAEEPDANEAAGDAPEGEGPSSASRGRLPPRAPAAATPSAAPPPRPAPPVPTPED
                     SGFYYRSGKPIPPTHPDHGPMHLLPRQEDGSFAAYYYCSHEELAIIGDGRLPAPPPRR
                     HRRDGFDAERQQQFVERYRDTGSLTDAARLTGISRSTFYNLINSPDGGAFRDAIDEAG
                     RGIDTILEATAFERCVNGQEEIVFYQGRRVGVRWKYDNKLLMQMLRARNPLKYAPLSE
                     IEGWLKRRGIAPPPDVDGALDRLAAAEAEWGRRLPGEPQPGQTTLAAPHTPLPPRERE
                     RAAEPPEGEGTAAEMPAIASTSSSSSTLPEEAQATAIASISSTSPAEPPTIASNSSTS
                     PAETSAIASNSSTLPAGSEPAPSPARAEPVEAHPFAPDSASSPTVRRPSPASWRPDSR
                     MKQMPTEFR"
     gene            2336..2542
                     /locus_tag="VGB57_04060"
     CDS             2336..2542
                     /locus_tag="VGB57_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0130557.1"
                     /translation="MPTGTVKFFNTQKGFGFIAPEGGGNDAFVHISAVERAGMNSLVQ
                     DQRVSYELETDNRGKTSAVNLQSA"
BASE COUNT          424 a          987 c          779 g          400 t
ORIGIN      
        1 tccgatcagc agccagacga tctcgcttgt caccgcgccc ttctaccccg atcggcgccg
       61 cggccgaagc cccaatcctt caccgcccgg ggaggcgccc gatcgcacgc cgccgccgcc
      121 gcctccgccg gcggccttga cgctggtgcg gccgcggcgc aacctcgccg gacaaccgag
      181 ggacccgcca tgatccacgc cgccgcgacg ctgcttcttt ccgccgtcca ggcgcccgat
      241 gcaccctcga ccgcggcgcc ctggcgcgac ctcggcctct cgcggggata tcatctcttc
      301 tacgatcccg cgccggtggc gcgtaacggc gagacgctga cggcgcgggt cctgacccgc
      361 ttcccgggcg ccgccgcaag cgagcccgcc tacgtcatcg gcacggtcga gatacgctgc
      421 ggcgccggcg aggcgcgggt cacgcgcacc accaaccacc tcgccgacgg cagcgtcgca
      481 agggaagaca ccgcgccgat gcccttcgct gcgatccggc ccgccaccct gttcgccacc
      541 ctccgccagg cgctctgctg agcgtccagc ggcggtgacg gtcgcatgcc tgtcaccgcg
      601 gctgcgacac aattggcacc gttcgctccc tcaagatgca tccgtttcgg acggcaggac
      661 gggcggacct cgctcccggc cggcccccgt acaaaccaaa gcgggatttt attttcggtc
      721 gctcttgcaa tgtagggttt catttgcttt accctcgtaa atggaacaga gcagaaaccc
      781 cgagtcgcgc tccggccgtg attggcggga ggattgggac ggccaggatg cgagcatccc
      841 ggacccgccg tgggagcccg acgcgatcga cctcctcctc catccggagc tggcgcaagg
      901 cgcggccgag gagcccgacg cgaacgaggc ggcaggcgac gcgccggagg gcgagggccc
      961 gtcctccgcg agtcgaggcc gtctcccgcc gcgggcaccg gcggcggcaa cgccctcggc
     1021 ggctcctccg ccacggcccg ctcctcctgt ccccacgccc gaggacagcg gcttctatta
     1081 cagaagcggc aagccgatcc cgccgacgca tcccgatcac ggcccgatgc acctcctccc
     1141 tcgccaggag gacggcagct ttgccgctta ttattattgc tcgcacgagg agctcgccat
     1201 catcggcgac ggacggctcc ccgcgccgcc gccgcgccgc caccgccgcg acggcttcga
     1261 cgccgagcgt cagcagcagt tcgtcgagcg ttatcgcgac accggcagcc tcaccgacgc
     1321 cgcccgcctc accggcatct cgcgcagtac cttctacaat ctcatcaaca gtcccgacgg
     1381 cggcgccttc cgcgacgcga tcgacgaggc gggccggggc atcgacacga tcctcgaggc
     1441 gaccgccttc gagcgctgcg tcaacggcca ggaggaaatc gtcttttatc agggccggcg
     1501 ggtcggcgtc cgctggaagt atgacaacaa gttgctgatg cagatgctgc gcgcccgcaa
     1561 tccgctcaaa tatgcgccgc tctccgagat cgagggctgg ctgaagcgcc gcggcatcgc
     1621 gcctccgcct gacgtcgacg gcgctctcga ccgcctcgcc gccgccgaag ccgaatgggg
     1681 ccggcgcctc cccggcgagc cccagcccgg gcagacgacg ctggccgccc cgcatacccc
     1741 tctcccacct cgggagaggg aaagggcggc ggagccgccc gagggtgagg gaacggccgc
     1801 cgagatgccc gctatcgcgt ccacttcgtc cagttcgtca actttgccgg aggaggcgca
     1861 ggccacggcg atcgcgtcca tttcgtccac ttcgccggcc gaacccccga cgatcgcgtc
     1921 caactcgtcc acttcgccag ctgaaacctc cgcgatcgcg tccaactcgt ccactttgcc
     1981 ggccggaagc gaaccggcgc cctctcccgc tcgggctgag cctgtcgaag cccatccctt
     2041 cgcccccgat tcggcctcat cccctaccgt ccgccgccca agccccgcct cgtggcgccc
     2101 tgactcgcga atgaagcaga tgccgaccga gtttcgttga aaaattgccc gtgaatatcg
     2161 tgcctcggag accgggattt gcttttcccg ccaaaatcgt gcataagaga agggctaaag
     2221 tcgcatgcgg cggatcttgc cttcttcggt cggtcttttc cttctcttga cgcctctaaa
     2281 gcccggcagg ccacgctggc cgcgccggat atctgaaata ccaaaggaaa acatcatgcc
     2341 gacaggcaca gtcaaattct tcaacaccca aaagggtttc ggtttcatcg cgcccgaggg
     2401 cggcggcaac gacgccttcg tccatatcag cgcggtcgag cgggccggga tgaacagcct
     2461 ggtccaggac cagcgcgtct cctacgagct cgagaccgac aatcgcggca agaccagcgc
     2521 cgtcaatctt cagtctgcct gatccgcttt cccctccctt cggctgcaag gccgggggga
     2581 gggcccattc
//
LOCUS       DASWNU010000204         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_318379, whole genome shotgun sequence.
ACCESSION   DASWNU010000204 DASWNU010000000
VERSION     DASWNU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318379"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VGB57_04065"
     CDS             <1..440
                     /locus_tag="VGB57_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130558.1"
                     /translation="RRPVRAAAPRTSTRLKAEPASFASAEAPAPLSSTLASMTTMLRR
                     LPRSAVAPRIVHRSFAYAQPAPRPAPAEFVHVGQVHAEAHLFTVRQTLSPEEVAEIKR
                     AVIQAAAEGRKVKLRVDRGEGSVGGGGHGNGFDINIQTLVKGE"
     gene            445..879
                     /locus_tag="VGB57_04070"
     CDS             445..879
                     /locus_tag="VGB57_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130559.1"
                     /translation="MNPNFTLGIAASLLLAGASGAALAHPHPDGDGEKVKNVVIIHDG
                     KRGEHLAGHSGERLRALAIHGKGGLVECNGGDKTVDEVTGDNGARTKVIICSHGAPTI
                     ADAERLEEALARISDNDHLNDEQKARIETALRSAIDRARSAR"
     gene            1089..1940
                     /locus_tag="VGB57_04075"
     CDS             1089..1940
                     /locus_tag="VGB57_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0130560.1"
                     /translation="MDVTGRVERLGSRIRRILAPNPSPFTYTGTQTYIVGEGEVAVID
                     PGPDLPGHVEALIAAVEGERVAAILCTHTHRDHSPASRPLAAATGASIVGCAPLSLED
                     KGPRSDEAFDADYRPDRILADGERLEAEGWTIEAVATPGHTSNHLCLALAGEGALFTG
                     DHVMGWSTSVVAPPDGDMAEYMASLDRLLGRDDRIYYPAHGPAVEDPHAHVRRLIEHR
                     RMRERQILRQLESGEGRIPAMVAEMYAEIDPRLFPAAGRSVLAHLVDLERRGLVRERG
                     ERWTLAD"
BASE COUNT          331 a          727 c          694 g          289 t
ORIGIN      
        1 cccggcggcc ggtccgcgcc gccgcgccgc ggacctccac ccggctgaag gcggaaccgg
       61 cgagcttcgc gtccgccgag gcgccggcgc cgctttcctc cactctcgca tcgatgacga
      121 cgatgcttcg ccggctcccc agatccgccg tcgcgccgcg aatcgtccac cgcagcttcg
      181 cctacgcgca gccggcgccg cgaccggctc cggcggagtt cgtccacgtc gggcaggtcc
      241 atgctgaagc gcatctcttc actgtccgcc agacgctcag ccccgaggaa gtcgccgaga
      301 taaagcgcgc cgtcatccag gccgcggcgg agggacgcaa ggtcaagctt cgagtcgatc
      361 gcggcgaagg ctcggtcggc ggcggcggcc atggcaacgg cttcgacatc aacatccaga
      421 cgctcgtaaa gggagaatga cccgatgaat cccaacttca ccctcggcat cgccgcttcg
      481 cttctgctcg ccggagcaag cggagccgcc ctcgcccatc cccatccgga cggcgacggc
      541 gagaaggtga agaatgtcgt gatcatccac gacggcaagc gcggcgagca ccttgccggc
      601 cactccgggg agcgcctccg ggcgctcgcg atccacggca agggcggcct ggtagagtgc
      661 aacggcggcg acaagacggt cgacgaggtc acgggcgata acggcgccag gacgaaggta
      721 atcatctgca gccatggcgc gccgaccatc gccgacgcgg aacgcctcga agaggcgctc
      781 gcccggatca gcgacaacga ccacctgaac gacgagcaga aggcgcggat cgagaccgcc
      841 ctgcgctccg cgatcgaccg ggcccgcagc gcccgctgag ggcaagcgcg actcggggaa
      901 agggcggagg gacctaagac ctccgccctt tctcttgccc gctcgtcatt gcgaggagcg
      961 cagcgacgca gcaatccagc tacggtttcg gtcagggcgc tggctggatt gcttcgcttc
     1021 gctcgcaatg acgggatcga gatgcctacc cgcttggttt tggacgcccg caatgccaca
     1081 tcggcgcgat ggacgtgacc ggcagggttg agcggctcgg cagccggatt cgccggatcc
     1141 tcgcgcccaa tccgtcgccc ttcacctata ccggcaccca gacctacata gtcggcgagg
     1201 gcgaggtcgc ggtgatcgat cccggccccg atctgccggg gcatgtggaa gctttgattg
     1261 cggcggtcga aggcgagcgg gtcgccgcga tcctgtgcac ccacacccac cgcgaccaca
     1321 gcccggcgag tcgtccgctt gccgcggcga ccggggcttc gatcgtcggc tgcgccccgc
     1381 tcagcctcga ggacaagggc ccgcgctcgg acgaggcgtt cgatgccgac tatcggcccg
     1441 accggatcct cgccgacggg gaacggctcg aagcggaggg ctggaccatc gaggcggtcg
     1501 cgactccggg gcacacgtcc aatcatctgt gcctcgcgct ggccggggaa ggcgctctgt
     1561 tcaccggcga tcatgtgatg ggctggtcga ccagcgtcgt cgctccgccc gacggcgaca
     1621 tggccgaata tatggccagc ctggaccggc tgctcggccg ggacgaccgg atatattatc
     1681 ccgcgcacgg ccccgccgtc gaagacccgc acgcccatgt ccgccgcctg atcgagcatc
     1741 gccggatgcg cgagcgccag atcctgcgcc agctcgaaag cggcgagggc agaattccgg
     1801 cgatggtggc cgagatgtac gccgagatag atccgcggct cttcccggcg gcgggccggt
     1861 ccgtgctcgc ccatctcgtc gacctcgagc ggcggggcct cgtcagggaa cggggggaga
     1921 gatggactct cgccgactga agctccacct ggtcctgccg gcgctgggcc tgcttctgct
     1981 cggcttcgcg ctgggctggg ggctccgccc cgtgccggcc gcggcccggc cggacgcgga
     2041 g
//
LOCUS       DASWNU010000205         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_112100, whole genome shotgun sequence.
ACCESSION   DASWNU010000205 DASWNU010000000
VERSION     DASWNU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112100"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VGB57_04080"
     CDS             <1..1239
                     /locus_tag="VGB57_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY0130561.1"
                     /translation="PDANPEGPFRMIGVLLDRDPFLGRILTGRIESGRLQVNMPIKAL
                     DVDNEEVETGRATKVFAMRGLERVPVETAQAGDIVAIAGLTRATVSNTIAEMAVTRAI
                     AARPIDPPTLAMSFAVNDSPYAGRDGDKVQSRVIRDRLEREAETNVAIRLTESAEKDA
                     FEVAGRGELQLGVLIETMRREGFELSISRPRVLFRDGPNGREEPYETVVIDVDEEHAG
                     TVVEKMAQRKGEMTDMRPSGGGKTRLTFSAPSRGLIGYHGEFLSDTRGTGIMNRLFEK
                     YGPYKGPITGRQNGVLISMEQGEAVAYALNMLEERGVLFISPGEKLYEGMIIGENAKP
                     QDLEVNPLKSKQLTNFRASGPKDESIRLTPPRRMTLEQAIAYIQEDELVEVTPKAIRL
                     RKRHLDPHERKRASRKAEAA"
BASE COUNT          315 a          529 c          560 g          210 t
ORIGIN      
        1 ccggacgcca atcccgaggg gccgttccgg atgatcggag tccttctcga ccgcgacccg
       61 ttcctcggcc ggatcctcac cgggcggatc gagagcggcc ggctccaggt caacatgccg
      121 atcaaggcgc tcgacgtcga caatgaagag gtcgagaccg ggcgcgcgac caaggtcttc
      181 gccatgcgcg ggctcgagcg ggtgccggtc gagaccgccc aggcgggcga catcgtcgcc
      241 atcgccgggc tgaccagggc caccgtctcc aacaccatcg cggagatggc ggtgacccgg
      301 gccatcgccg cccggccgat cgatccgccg accctggcga tgagcttcgc cgtcaacgac
      361 agcccctatg cgggccgcga cggcgacaag gtgcagagcc gggtgatccg cgaccgcctg
      421 gagcgcgagg ccgagaccaa cgtcgccatc cggctcaccg agagcgccga gaaggacgcc
      481 ttcgaggtgg ccggccgggg cgagctccag ctcggagtgc tgatcgagac gatgcggcgc
      541 gaaggcttcg agctttcgat cagccgcccg cgggtcctct tccgcgacgg cccgaacggc
      601 cgcgaggagc cctatgagac ggtcgtcatc gacgtcgacg aggagcatgc cggaaccgtc
      661 gtcgagaaga tggcgcagag gaagggcgag atgaccgaca tgcgcccctc gggcggcggc
      721 aagacccggc tgaccttctc ggcgccgtca cggggcctga tcggctatca cggcgagttc
      781 ctctccgaca cccgcggcac cgggatcatg aaccggctgt tcgagaaata cggcccctac
      841 aagggcccga tcaccggccg gcagaacggg gtgctgatct cgatggagca aggcgaggcc
      901 gtcgcctacg cgctcaacat gctcgaggaa cggggcgtgc tgttcatctc gcccggcgag
      961 aagctctacg agggcatgat catcggcgag aacgccaagc cccaggatct ggaggtcaat
     1021 ccgctcaagt ccaagcagct caccaacttc cgcgcctcgg ggccgaagga cgagagcatc
     1081 cggctgaccc cgccgcggcg gatgacgctg gagcaggcca tcgcctacat ccaggaggac
     1141 gagctcgtcg aggtgacccc caaggcgatc cggctgagga agcggcatct ggacccgcac
     1201 gagcggaagc gggcgagccg gaaggcggag gccgcttagc cgccgagtgc agggttccca
     1261 tcaaagtact ggtttgatgg ggcccgcagc gccgccgacg cgaaaacgcg tcgccgaagc
     1321 agcaccaaga gacggcgtcc ccggtcggcc ggcggccggc ggagcagagt cgttcgacgt
     1381 cacgaattcg gttcgtgacg cctgcactgt gcaaatgggt tcacgcggag gcgcggaggc
     1441 gcggagaaat ggggttcact cgaagccgca aaggcgcgaa ggctcggcgg agggcgtgat
     1501 cgcggtgaga acgccaactt acgcaagtga ctgccatcta atcgccgaat aattgtcacc
     1561 ggactggctc cggccaaacg atagaggatg agacgaacat gccgcccttc aagg
//
LOCUS       DASWNU010000206         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_475855, whole genome shotgun sequence.
ACCESSION   DASWNU010000206 DASWNU010000000
VERSION     DASWNU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475855"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VGB57_04085"
     CDS             <1..548
                     /locus_tag="VGB57_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0130562.1"
                     /translation="LAYQGLGHGLEADAAGVRLVVGKAEQALVAQSCDKNFGVYRERV
                     GNLFVKSASAKAAQVVYGNLLQLSRTMWSMPPDHGAAIVHIVLEDPELRSDWNSELKE
                     MCGRIRALRSRLAAYDERLAYIDGQNGMFSMLPLSQAQVLELREEKGIYMANSGRFNV
                     VGLSDDNVDLFAAAVVEKMDG"
     gene            541..2622
                     /locus_tag="VGB57_04090"
     CDS             541..2622
                     /locus_tag="VGB57_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0130563.1"
                     /translation="MAESGEKVRNRQKSPHSAYDWRRVAYVVQVSRAMDKLEEERLVP
                     ERKVLYQFSARGHDMAQAMLGSRLTHRRDATCGYYRSRPLLLALGVPVEDALGSGMGR
                     EGGYSDGRDIGVVFNYPNPGGAPALPMSGGVGAQYTPTAGWAQAIEYYRNELGDNSYD
                     GAIAVVLGGDASCATGGFWSALTIATTQKLPMLFYVEDNGFGISVPSSFQTPGGDIAR
                     NLASFGNLAIFAGDGTEPGEAAELIERAVGHVRGGKGPALLRLTVPRLQGHSFQDTQT
                     YKGEETIRQEWTRDPLPKLKAHLVDSLLTEAEWNEIEARAQADVEAARQTAEARGVSD
                     PERVLDHVFYDGEMQKMGGQWTHGYRAPHSHEEPKPEGQRINMVTAIRRTLDHELSVN
                     PRVLLFGEDIGPKGGVHAVTLGLQEKYGAGRVFDTSLNEEGIIGRAVGMAIAGLMPVP
                     EIQFRKYAEPATEQINDCGTMRWRTNNRFAAPMVLRIPGGFFKCGDPWHSQTNEVAFV
                     HNPGWQVAVPSNAEDAVGLLRTALRGNDPVVFFEHRAMLDDAWARRPYPGDGYALPFG
                     RAKKVRQGDSITIVTWGAMVPRCEEAARDVSADVIDLRTLMPWDRETVLDSVRRTRRC
                     LIVHEDLRTGGFGAEIAAVVADEAFLDLDAPVARVTMPDIPSPHNPVLLNWAVPSVDR
                     IRAKIDELVGF"
BASE COUNT          471 a          891 c          973 g          426 t
ORIGIN      
        1 acctcgccta tcaggggctc ggccacggcc tggaggccga tgcggcgggc gtgcggctcg
       61 tcgtcggcaa ggcggagcag gcgctggtgg cgcagagctg cgacaagaat ttcggggtct
      121 atcgcgagcg ggtcggcaat ctgttcgtca agtcggcgag cgcgaaggcg gcgcaggtgg
      181 tctacggcaa cctcctccag ctttcgcgga ccatgtggtc gatgccgccc gatcatggcg
      241 ccgcgatcgt ccacatcgtg ctcgaggatc ccgagcttcg ctccgactgg aactcggagc
      301 tcaaggaaat gtgcggcagg atcagggcgt tgcgctcgag acttgccgcc tatgacgagc
      361 ggctggccta tatcgatgga cagaacggga tgttctcgat gctgcccctg agccaggccc
      421 aggtgctgga gctgcgcgag gagaagggca tctacatggc gaattccggt cgtttcaacg
      481 tcgtcggcct ctccgacgac aatgtcgatc tgttcgccgc ggcggtggtg gagaagatgg
      541 atggctgaaa gtggagagaa ggttaggaat cgccagaaat cgccacactc cgcttatgat
      601 tggcggcggg tcgcctatgt cgtccaggtc agccgggcga tggacaaact cgaggaggag
      661 aggctcgtcc ccgagcgcaa agtgctctac cagttcagcg cgcgcggcca cgacatggcc
      721 caggcgatgc tcggaagccg gctcacccac cgccgcgacg ccacctgcgg ctattacagg
      781 tctcggccgc tgctgctggc tttgggcgtt ccggtcgagg acgctctcgg ctccggaatg
      841 ggccgggaag ggggctattc ggacggccgg gatatcggag tggtcttcaa ctaccccaac
      901 cccggcggcg cgccggcact gcccatgtcg ggcggagtgg gggcgcaata tacgccgacc
      961 gcgggctggg cccaggcgat cgaatattat cgcaatgagc tgggcgacaa ctcctacgac
     1021 ggagcgatcg cggtggtgct ggggggcgac gcttcctgcg cgaccggcgg cttctggtcg
     1081 gcgctgacca tcgccaccac ccagaagctg ccgatgctct tctacgtcga ggacaatggc
     1141 ttcgggatct cagtgccgtc cagcttccag accccgggcg gggacatcgc ccggaatctg
     1201 gcgagcttcg gcaacctcgc gatcttcgcc ggcgacggca ccgagccagg ggaggcggcg
     1261 gagctgatcg agcgggcggt cggccatgtc cggggcggca aggggccggc gctgctcagg
     1321 ctgaccgtgc cgaggcttca gggccacagc ttccaggaca cccagaccta taagggcgag
     1381 gagacgatcc gccaggaatg gacgcgcgat ccgctgccca agctcaaggc gcatctcgtc
     1441 gattcgctgc tgaccgaggc ggagtggaac gagatcgagg cccgggccca ggcggacgtc
     1501 gaggcggcgc ggcagaccgc cgaggcgcgc ggggtctccg atcccgagcg ggtgctcgac
     1561 catgtcttct acgacggcga gatgcagaag atgggcgggc aatggaccca cggctaccgt
     1621 gccccccaca gccacgagga acccaagccc gagggtcagc ggatcaacat ggtgacggcg
     1681 atccgccgga cgctcgacca cgagctctcg gtcaacccgc gcgtgctcct gttcggcgag
     1741 gatatcgggc ccaagggcgg ggtccacgcg gtcaccctgg gtctgcagga gaaatatggc
     1801 gccggccggg tcttcgacac cagcctcaac gaggagggga tcatcggccg agccgtcggg
     1861 atggcgatcg ccggcctgat gccggtgccg gaaatccagt tccgcaaata tgccgagccc
     1921 gcgaccgagc agatcaacga ttgcgggacg atgcgctggc gcacgaacaa caggttcgcg
     1981 gcgccgatgg tgctgaggat ccccggcggc ttcttcaaat gcggcgatcc ctggcacagc
     2041 cagaccaacg aggtcgcgtt cgtccacaat cccggctggc aggtggcggt gccgtccaac
     2101 gccgaggacg cggtcggcct gctccgcacc gcgcttcgcg gcaacgatcc cgtcgtcttc
     2161 ttcgagcacc gggcgatgct cgacgatgcc tgggcgcggc gtccctatcc gggcgacggc
     2221 tacgcgcttc cgttcggacg ggcgaagaag gtccgccagg gcgactccat caccatcgtc
     2281 acctggggcg cgatggtgcc gcgctgcgag gaagcggcca gggacgtgtc ggcggacgtc
     2341 atcgacctca ggaccctgat gccctgggac cgggagacgg tgctcgacag cgtccgccgg
     2401 acccgccgct gcctgatcgt ccacgaggat ctgaggaccg gcggcttcgg cgccgagatc
     2461 gccgcggtgg tcgccgacga ggccttcctc gacctcgacg ccccggtggc gcgggtgacg
     2521 atgcccgaca ttccgagccc gcataatccg gtgcttctca actgggcggt gccgagcgtg
     2581 gaccggatcc gggcgaagat cgacgaatta gtggggtttt gacgatgggc gggatcacgt
     2641 cgccccagcg caggctgggg ccccagcgag atcaggcacc gagcctttca ccccccgggg
     2701 ccccagcctt cgctggggcg acgttcacgc atgaccgccc cgcccacggc cgtaatcttc
     2761 g
//
LOCUS       DASWNU010000207         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_345079, whole genome shotgun sequence.
ACCESSION   DASWNU010000207 DASWNU010000000
VERSION     DASWNU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345079"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..654
                     /locus_tag="VGB57_04095"
     CDS             91..654
                     /locus_tag="VGB57_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0130564.1"
                     /translation="MGLWIALGLLVLALLYAIIVYNRLVRLRALTREGFSGITVQLRR
                     RADLVPNLVGTVEGYATHEREVLTEVTAHRTDPTRAGSVEATAQADNAFGAMLGRLMA
                     VAEDYPDLKADENFRALQDELAEIEEELQGARRYYNATVRDLNTRVQSFPDVLLARPM
                     GFSEQPFYEDGDASIQTAPRVSFGRAG"
     gene            654..2594
                     /locus_tag="VGB57_04100"
     CDS             654..2594
                     /locus_tag="VGB57_04100"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY0130565.1"
                     /translation="MRRLLLPLLAALALLWPAAASAEERILHYLSDVLVQRDGSLDVT
                     ETIRVRSESALIDHGIYRDFPTRYTGRTGAQVRVGFEVVNVQRNGADEPWHTERQGNG
                     VRVYMGSAETNVPPGEHVYSIRYRTTRQLGFFKDHDELYWNATGNGWVFPIDVAEARI
                     RLPQPVPFGQRAFYTGPQGSTATNAEVVSEAPGEIVFRTTEPLAPGEGLTVAPAWPKG
                     VVTPPTPPSAAGHWLRDNGPPAAGVLALLGVLAYYFHAWRRAGRDPKPGTIVPIFSPP
                     DGLSAAAMRYITEMDADSRAFAAALVQLGVGGHLRLTEEEVGFFFGRKKTTIEKTAGR
                     TDLEEPEASMLSRLFAEGDSILMEQKNHATFSGAKDALEAGLKRVHEGRLFVRNRIWA
                     VRGLVLMVGALALPAAAVLLASPGDYGRNDILVALAALLLPAAALGLYVLSGGKYGAM
                     AMSAKLSAAFLGVIGLVAGVVTIGNAVEGIVAYGEIRRLIPLAIPLLALPVVLTAFSW
                     MAAPTREGRVVLDRIAGFRHYLSVAEEERLETMHPPEKTPELFERYLPYAIALEVEND
                     WASRFTSVLAAAAASGQAQPMGWYSGHSDPWTDPGDFADSVGSSLASTISSASAAPGS
                     SGGSGGGGSSGGGGGGGGGGGW"
     gene            complement(2535..3374)
                     /locus_tag="VGB57_04105"
     CDS             complement(2535..3374)
                     /locus_tag="VGB57_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0130566.1"
                     /translation="MGEWTAIVLAGRRPGEDCFAASHGVAAKALIRAGGEPMLGRVAR
                     ALLSSPSIGRIVVLAQEAETLLTGDLEWMAAEPRIATARSGDGISASVGGLAGTAGAP
                     YPVLVTTADHALLQPEMVEAFIAGAAGVDVAFAMVERKTVEKAHPDTRRTWLKAANGH
                     FSGANLFALLTPASARGTEFWARAEKERKRTLKLLSFLGLGIFLRAVTRTISLEAAAE
                     RVGRNAGFRLKAVLLPFAEAAIDVDKPSDLELAERILAARAASPAAAAAASAAASGRS
                     AAP"
     gene            complement(3535..>4895)
                     /locus_tag="VGB57_04110"
     CDS             complement(3535..>4895)
                     /locus_tag="VGB57_04110"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130567.1"
                     /translation="AEKAVARESMILWDDLIAPSIVEKVGKGADIVLANTTTGPAQAA
                     AYLPGNGEKRQGDVWTMDPSVNWSNGWLQYDSYGRTTLIHEVGHSLGLPHPGDYNFSD
                     DKNGDGVPDPITYVGDAFYAQDSKQYTIMSYFGAWETGGQVINPALRQLANAQTPLLD
                     DILAIQALYGADPTTRTGDTNYFSNSNAGNGVYDLALNPYPYLSIYDAGGEDTFDFST
                     ANRSVFIDLRPGAFSSAAAGAVSLEQANAIVAEYNEATSAAKGDLPLFTSQAQVDAEA
                     NFAGQLGHNRVLNDTGVAGVFATSHRNISIAYNTVIENANGGSARDYLYGNDVSNKLN
                     GNGGNDVLDGAKGNDILTGGAGADEFRVSEIGFSDEIVDFTSGLDKIRLSEIDSNSGV
                     AGNQAFTFVGNNAFSGTAGELRTYSQGGDRFVAGDVNGDGLADFTINVNNANVVVTDF
                     FL"
BASE COUNT          708 a         1678 c         1673 g          836 t
ORIGIN      
        1 cgcactggat tgcttcgctt cgctcgcaat gacgggccct aggcggagcc tgcctcctgc
       61 cctagaaccg cccgcaaggg ggatcttgcc atgggattat ggatcgcgct cggcctgctg
      121 gtgctggccc tgctctacgc aatcatcgtc tacaatcgcc tcgtgcgcct gcgcgcgctt
      181 accagggaag ggttcagcgg gatcaccgtc cagctccgcc gccgggcgga tctggtgccc
      241 aacctggtcg gcacggtcga aggctatgcc acccatgagc gcgaggtgct gaccgaagtc
      301 accgcccacc gaaccgatcc caccagagcg gggagcgtcg aggccacggc gcaggccgac
      361 aacgccttcg gtgccatgct cggccggctg atggcggtcg ccgaagacta tcccgatctc
      421 aaggcggacg agaatttccg ggcgctccag gacgagctcg ccgagatcga ggaggaactg
      481 cagggcgccc ggcgctatta caacgccacc gtccgcgatc tcaacacgcg cgtccaatcc
      541 ttcccggacg tgcttctcgc ccgcccgatg ggcttctccg agcagccgtt ctacgaggat
      601 ggcgacgcaa gcattcagac ggcgcccagg gtcagcttcg gccgggccgg ctgatgcggc
      661 ggctcctgct gccgctgctg gcggcgctcg ccttgctctg gccggccgcc gcctcggccg
      721 aggagcgcat ccttcattat ctgagcgacg tcctcgtcca gcgcgacggg tcgctcgacg
      781 tcaccgagac gatccgggtc cgctcggaaa gcgctctgat cgaccacggc atctatcgcg
      841 atttcccgac ccgctacacc ggtcgcaccg gcgctcaggt gcgtgtcggg ttcgaagtgg
      901 tgaacgttca gcgcaacggc gccgacgagc cctggcacac ggaacgccag ggcaatggcg
      961 tccgggtcta tatgggctcg gccgagacga acgtcccgcc gggcgagcat gtctattcga
     1021 tccgctaccg gaccacccgc cagctcggct tcttcaagga tcatgacgag ctctactgga
     1081 acgccaccgg caatggctgg gtgtttccga tcgacgtcgc cgaagcgcgg atccggctgc
     1141 cccagccggt gcccttcggc cagcgtgcct tctacaccgg cccccagggc tcgaccgcca
     1201 cgaatgccga ggtggtttcc gaggcgcccg gcgagatcgt cttccggacc accgagcccc
     1261 tcgcgcccgg ggaagggctg acggtcgcgc ccgcctggcc gaagggagtc gtgaccccgc
     1321 cgacgccgcc ctccgccgcc ggtcactggc tccgggacaa cggcccgccg gccgcgggcg
     1381 tgctcgccct cctcggggtg ctggcttatt atttccacgc ctggcgccgc gccggccggg
     1441 atccgaagcc gggcacgatc gtccccatct tcagcccgcc cgacggcctt tccgccgcgg
     1501 cgatgcgcta catcaccgaa atggacgccg acagccgcgc cttcgccgcc gctttggtcc
     1561 agctcggagt cggcggccac ctgcgcctca ccgaggagga ggtcgggttc tttttcggcc
     1621 gcaagaagac gacgatcgag aaaaccgccg gccggaccga tctggaggaa ccggaggcct
     1681 cgatgctgtc ccggctcttc gcggaaggcg attccatcct gatggagcag aagaaccacg
     1741 ccaccttcag cggcgccaag gacgccctcg aggccgggct gaagcgggtc catgagggcc
     1801 ggctgttcgt ccgcaaccgc atctgggcgg ttcgcggcct ggtgctgatg gtcggggcgc
     1861 tcgccctccc ggcggcggcg gtcctgctgg cgtcgcccgg cgattatggg cgcaacgaca
     1921 tattggtcgc ccttgccgcc ctgctccttc cggcggcggc tctgggcctc tatgttctct
     1981 ccggcggcaa atacggggcg atggccatgt cggccaagct ctccgccgcc ttcctcgggg
     2041 tgatcggtct cgtcgcgggt gtcgtcacca tcggcaacgc ggtggaggga atcgtcgctt
     2101 atggcgagat ccggcgtctc atcccgctgg cgatcccgct tctggccctg ccggtggtgc
     2161 tcacggcctt cagctggatg gccgcgccga cccgcgaagg gcgggtcgtg ctcgatcgga
     2221 tagcgggctt ccgccactat ctttcggtcg ccgaggaaga gcgcctcgag accatgcacc
     2281 cgcccgagaa gactcccgag ttgttcgagc gctatctgcc ctacgcgatc gccctcgagg
     2341 tcgagaatga ctgggcctcg cgcttcacct ccgtgctcgc cgccgcggcg gcctcggggc
     2401 aggcgcagcc catgggctgg tattcgggcc attccgatcc gtggaccgac cccggcgact
     2461 tcgcggacag cgtcggctcc tcgctggcga gcaccatctc ctccgcctcg gccgcgcccg
     2521 gatcgagcgg cggatcaggg ggcggcggat cgtccggagg cggcggcgga ggcggcggcg
     2581 gcggcggctg gtgaagcggc gcgggcggcg aggatccgct cggccagctc gaggtccgac
     2641 ggcttgtcga cgtcgatcgc cgcctccgcg aagggcagca ggaccgcctt cagccggaag
     2701 cccgcgttgc ggccgacgcg ctcggcggcg gcttccagcg atatcgtgcg ggtgacggcg
     2761 cggaggaaga tgccgagccc gaggaagctc agaagtttca gggtgcgctt gcgttccttc
     2821 tcggcccgcg cccagaattc ggttccccga gccgatgccg gcgtcagcag ggcgaagagg
     2881 ttggcgccgc tgaaatggcc gttcgcggcc ttgagccagg tccgcctggt gtcgggatgg
     2941 gccttctcga cggtcttgcg ctccaccatg gcgaaggcga cgtcgacccc ggccgccccg
     3001 gcgatgaagg cctccaccat ctcgggctgg agcagggcgt ggtcggcggt ggtgaccagg
     3061 acggggtagg gcgcgcccgc ggtgccggcg agtccgccga cgctggccga aatgccgtct
     3121 ccggaccggg cggtcgcgat ccggggctcg gccgccatcc attcgaggtc cccggtcagg
     3181 agcgtttccg cctcctgggc gagcaccacg atccggccga tcgaggggga tgagagcagg
     3241 gcccgcgcca cccggcccag catcggttcg ccgccggcgc ggatcagcgc cttcgccgcg
     3301 acgccgtgcg aagccgcgaa gcaatcctcc cccggccggc gcccggcaag cacgatcgcg
     3361 gtccattccc ccatgggtgc gagttcatct cagctaggcc gcccgggggc catcgaaaat
     3421 cgtccgacgg tcgcgttaat cgtcccgccg ttgccgatcc gccacgccgc cgggggcgaa
     3481 aagaaagggc ccgccgcatg gcggcaggcc ctcccttcgc gtcgatctcg cttcctagag
     3541 gaagaagtcg gtcaccacga cattggcgtt gttgacgttg atcgtgaagt cggcgagccc
     3601 gtcgccgttc acgtcgccgg cgacgaacct gtcgccgccc tggctgtagg tcctgagctc
     3661 gccggccgtg ccgctgaagg cgttgttgcc gacgaacgtg aaggcctgat tgccggcaac
     3721 gccgctgttg gagtcgatct cgctcagacg gatcttgtcg agcccgctgg tgaagtcgac
     3781 gatctcgtcg ctgaagccga tctcgctcac ccggaactcg tccgcgccgg cgccgccggt
     3841 gaggatgtcg ttgcccttgg cgccgtccag cacgtcgttg ccgccgttgc cgttcagctt
     3901 gttcgagacg tcgttgccgt agagatagtc tcgcgccgag ccgccgttcg cattctcgat
     3961 gaccgtgttg taggcgatcg agatgttgcg gtggctggtg gcgaagacgc cggcgactcc
     4021 ggtatcgttc aggactcggt tatggccgag ctggccggcg aaattggcct ccgcgtcgac
     4081 ctgcgcctgg ctggtgaaga gcggcaggtc gcctttggcg gcgctggtgg cttcgttgta
     4141 ctcggccacg atcgcattcg cctgctccag gctcaccgcc ccggcggccg ccgagctgaa
     4201 cgcccccggc cggaggtcga tgaacacgct ccggttcgcc gtcgagaaat cgaacgtgtc
     4261 ctcgccgccg gcatcgtaga tcgacagata gggatagggg ttgagggcga gatcgtagac
     4321 cccgttgccc gcgttcgagt tggagaaata gttggtgtcg ccggtgcggg tcgtcggatc
     4381 cgcgccatag agggcctgga tcgcgaggat gtcgtcgagc agcggagtct gcgcgttggc
     4441 gagctgcctc agcgccgggt tgatcacctg gccgcccgtc tcccacgctc cgaaatagga
     4501 catgatcgta tattgcttgg agtcctgggc gtagaaggcg tcgccgacat aggtgatcgg
     4561 atcgggcacc ccgtcgccat tcttgtcgtc gctgaaatta tagtcgccgg gatgaggcag
     4621 gccgagcgaa tggccgactt cgtggatcag ggtcgtccgg ccgtagctgt catattgaag
     4681 ccagccattg gaccagttga cgctggggtc catggtccag acgtcgccct ggcgcttctc
     4741 gccgttgccc ggaagatagg cggcggcctg cgccgggccg gtggtcgtgt tggccaggac
     4801 gatgtcggcg cccttgccga ccttctcgac gatcgacggc gcgatgaggt cgtcccaaag
     4861 gatcatcgac tcgcgggcga cggccttctc cgcct
//
LOCUS       DASWNU010000208         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_215858, whole genome shotgun sequence.
ACCESSION   DASWNU010000208 DASWNU010000000
VERSION     DASWNU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215858"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGB57_04115"
     CDS             <1..369
                     /locus_tag="VGB57_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0130568.1"
                     /translation="LIPDSGGTWVLPRLVGQARALGLALTAEPLSAEKAAEWGLIWKA
                     VEDERLDEEVDALVARFAEAPTRGLARIKKMIRESWGHSLDEELDLQRDAMRELGFGD
                     DYREGVAAFMEKRKPNFRGK"
     gene            482..2500
                     /gene="paaZ"
                     /locus_tag="VGB57_04120"
     CDS             482..2500
                     /gene="paaZ"
                     /locus_tag="VGB57_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEY0130569.1"
                     /translation="MKTLKLLNYAQDRWYGAEGGLAPVESAVTGEVVAETGSGGLDFA
                     AMLDHARKVGGPALRRLTFHERAHMLKALANAIMARKEELYELNYETGATRTDGWIDI
                     EGGAGTLFSFSSKGRRELPNGRILIDGQTEPLSKGGTFVGQHVYTSLQGVAVHINAFN
                     FPVWGMLEKLGPTLLAGVPAIVKPASATGYLAEAAFRIMIEADVLPPGALQLVMGGVG
                     DLFDHLTLQDVVSFTGSAQTAMKLQTHPVIARESVRFIAERDSLNASVLGPDAAPGTP
                     EFDLFIKEIAREMTIKAGQKCTAIRRAMAPAEHLDSVQAALIERLGKARIGNPREEGV
                     TMGALASRSQLRSVREAVAELARSARIVSGDPDLSPVEGNGSFISPILLRSDDPWGSA
                     AIHDVEAFGPVSTIMPYRDLRDAVALANRGMGSLALSLFTFDPAVAEEFVLGAGAYHG
                     RMVILDRTSARESTGHGSPLPVLVHGGPGRAGGGEEMGGIRGVTHYMQRTALQGSPAV
                     LSSVVKQWLPGAPKPEGDRHPFRKRISELEIGYTLKTVSRTVSLDDIEHFAAFTGDNF
                     YAHMDEEAAKANPIFDGRVAHGYLILSFAAGLFVDPDPGPVLANTGLEGLRFQTPLYP
                     GDSMRVELTVKSKSVRNEEMGEVRWAVYVFNQKDEVVASYELLTMNAP"
BASE COUNT          449 a          945 c          857 g          422 t
ORIGIN      
        1 ctcatccccg acagcggcgg cacctgggtg ctgccacgcc tcgtgggcca ggcgcgcgcc
       61 ctcggccttg cgctgacggc cgagccgctt tcggcggaaa aggcggccga atggggcctg
      121 atctggaagg cggtcgagga cgagcggctc gacgaggagg tcgatgcgct ggtcgcccgc
      181 ttcgccgagg ctccgacccg ggggcttgcg cgaatcaaga agatgatccg cgaaagctgg
      241 ggccacagcc tcgacgagga gctcgacctc cagcgcgacg caatgcgtga gctcggcttc
      301 ggcgatgatt atcgcgaagg cgtcgcggcg ttcatggaga agcggaagcc gaatttcagg
      361 gggaagtgag ctcccgtcat cccccatcgt caccctgaac ttgtttcagg gtccagtttc
      421 gagcatcgcg accttcatcg aactggatgc tgaacaagtt cagcatgacg ggaaaagaga
      481 catgaaaacg ctgaagctgc tcaattacgc ccaggaccgc tggtacggcg ccgaaggcgg
      541 cctcgccccg gtcgagagcg cggtcaccgg cgaggtggtc gccgagaccg ggagcggcgg
      601 cctcgatttc gccgccatgc tcgaccacgc ccgcaaggtc ggcgggccgg cgctccgccg
      661 cctcaccttc cacgagcgcg cccatatgct gaaggcgctc gccaacgcga tcatggcccg
      721 caaggaggag ctgtacgagc tcaattacga gaccggcgcc acccgaaccg acggctggat
      781 cgacatcgaa ggcggcgccg gcaccctctt ctctttctcc tccaagggcc ggcgcgagct
      841 tcccaacggg cggatcctga tcgacgggca gacggagcct ttgtcgaagg gcggcacctt
      901 cgtcggccag cacgtctaca cctcgctcca gggcgtcgcc gtacacatca acgccttcaa
      961 cttcccggtc tggggcatgc tggaaaagct cggccctacc ctgctcgcgg gagtcccggc
     1021 catcgtcaag ccggcgtcgg cgaccggcta tctcgccgaa gcggcgttcc ggatcatgat
     1081 cgaggcggac gtgcttcccc ccggcgcgct tcagctcgtg atgggcgggg tcggcgacct
     1141 gttcgaccat ctcacccttc aggatgtcgt cagcttcacc ggctcggccc agaccgcgat
     1201 gaagctccag acgcatccgg tgatcgcccg cgagagcgtc cgcttcatcg ccgagcgcga
     1261 cagcctcaac gcctcggtcc tcggccccga cgccgcgccg ggcacgcccg agttcgacct
     1321 gttcatcaag gagatcgctc gggagatgac gatcaaggcg gggcagaaat gcaccgccat
     1381 ccgccgcgcc atggcgccgg ccgagcatct cgactcggtc caggcggccc tgatcgaacg
     1441 gctcggcaag gcccggatcg gcaatccccg cgaggagggc gtcaccatgg gcgccctcgc
     1501 cagccggtcc cagctccgct cggtacgcga ggcggtggcg gagctggcca gatcggcccg
     1561 gatcgtttcc ggagatccgg atctctcgcc cgtcgagggc aacggctcct tcatctcccc
     1621 catcctgctc cgctccgacg atccctgggg atcggccgcg atccacgacg tcgaggcgtt
     1681 cgggccggtc tcgacgatca tgccctatcg cgacctccgc gacgccgtcg cgctcgccaa
     1741 tcgcggcatg ggcagcctcg ccctctcgct cttcaccttc gacccggcgg tcgccgagga
     1801 gttcgtgctc ggcgccggcg cctatcacgg ccgaatggtg atcctcgacc gcaccagcgc
     1861 cagggaatcg accggccacg gctcccctct ccccgtcctc gtccacggcg gccccggccg
     1921 cgccggcgga ggcgaggaga tgggcggcat ccgcggggtc acccattata tgcagaggac
     1981 cgcgcttcag gggtctccgg cggtgctgtc gagcgtggtc aagcaatggc tgcccggcgc
     2041 tcccaagccc gagggcgatc gccacccctt ccgaaagcgc atctcggagc tggagatcgg
     2101 ctacacgctc aagaccgtga gccgcaccgt cagcctcgac gatatcgagc atttcgccgc
     2161 cttcaccggc gacaatttct acgcccatat ggacgaggaa gcggccaaag cgaacccgat
     2221 cttcgacggc cgggtcgccc acggctacct catcctgagc ttcgcggccg ggctgttcgt
     2281 cgatcccgat ccgggccccg tcctcgccaa taccggcctc gaggggcttc gcttccagac
     2341 gccgctctac ccgggcgatt cgatgcgggt cgagctgacg gtcaaatcga agagcgtccg
     2401 aaacgaggag atgggcgagg tgcgctgggc cgtctatgtg ttcaaccaga aggacgaggt
     2461 ggtcgcttcc tacgagctcc tcaccatgaa cgcgccctag ccgtgaccca ccaccccgat
     2521 cccgcctgcg ttctggtcct cgtctcgccc gacggggcgc tgcgcctgct ggtgcacaag
     2581 gacgagggcg acttttccat cggcttcgag ggctatgagt ggcacaccca cggcgacctt
     2641 cttgtcaaag acggcgaaac gcaggaggaa gcg
//
LOCUS       DASWNU010000209         3643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_475925, whole genome shotgun sequence.
ACCESSION   DASWNU010000209 DASWNU010000000
VERSION     DASWNU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475925"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1493)
                     /locus_tag="VGB57_04125"
     CDS             complement(66..1493)
                     /locus_tag="VGB57_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0130570.1"
                     /translation="MKKLALLLPVLLLAAPASAATPREIADAVSQERLRAIVEKLVSF
                     GTRHTLSSQTDPKRGIGASLRWTEAEFGRYSRACGGCLRVVTPSDVVTGRRVPNPTRV
                     TNVFAVQRGSADPNRVVIISGHIDSRVTDPMNATSDAPGANDDGSGTAAVIEAARVLS
                     KHKFPYTIVYAALSGEEQGLLGGKIMADYAKAQGWEVVANLNNDIIGNSCSSDGVCDD
                     AHVRVFSEGPRWQGHEDLAARIRSLGGENDAPSRNISRWLDDLAADLSLGLDVRQIWR
                     NDRFGRGGDHTEMLNAGFPAVRITVAIENYNWQHQDLRVENGIQYGDTIDKMDFPYLT
                     KVVKLNVAALAALASAPQPPEPRVEGAVSTDTNIAWNPVRGAEAYIVRWRRTDAANWE
                     HSLRVDYAGLDRCPARPAAPNAAPNAVGDRHVATCVQALKGVRVDDYVFGVSSVSRDG
                     YESPVASAVPGGAFEPYTPPPPPQP"
     gene            1615..2493
                     /locus_tag="VGB57_04130"
     CDS             1615..2493
                     /locus_tag="VGB57_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0130571.1"
                     /translation="MLELADVSHVYPNGTRALDHVTLSIPRGMYGLLGPNGAGKSTLM
                     RNVATLQTPTEGSIRFGDIDVIAEPEKLRRTLGYLPQDFGVYPRVSAYDMLDHMAVLK
                     GIASAPARKQTVEGLLHQVNLWDVRKKALAGFSGGMRQRFGIAQALIGNPELIIVDEP
                     TAGLDPEERNRFLNLLAEIGENVVVILSTHIVEDVSDLCPRMAVLAGGRIQLEGAPLE
                     LIRSTRGRIWMKVIERGELELYREKYELISTRLFAGRTVIHILSDTDPGDGFASVEGG
                     LEDVYFTTLVQSRRAA"
     gene            2507..>3643
                     /locus_tag="VGB57_04135"
     CDS             2507..>3643
                     /locus_tag="VGB57_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0130572.1"
                     /translation="MFLKIARFELRYQVKNPVFWVAAVMFFLLCFGFMASEAVSGGAS
                     GNLHRNSPVQLTQIQTTFSLFFMFVTTAFVANIVVRDDETGFGPIIRSTPITRLQYLG
                     GRFAGAVAAASLAYLFIALGLWVGSIMPWVDPETLGPNRLSYYLYGIAVFGVPNLLVT
                     GAIFFTAATLTRSMMWTYVAVVVFFLAWLILTGIGQAKPELRDTLALVEPFGVGAFGN
                     AVKYWTTAERNIGLPPVEGAILMNRLIWLGIASAFLAFAIARYRFAEPALSKKRRKLE
                     AAQAAPAPVPSPAPAAALPDAGGSAAWAQLRARTRFEMKQVVRSPAFFVLVALGMINT
                     VANLWFAGEMFGTPTLPVTRWVIPMLAGSFGIFSIIIGIYYSGEL"
BASE COUNT          566 a         1230 c         1195 g          652 t
ORIGIN      
        1 accttctcgt tgcgctcggg aaggaagaag atgggtgccg gatcaagtcc ggcatgacga
       61 atgcgtcacg gctgtggcgg aggcggaggc gtgtagggct cgaacgcccc gcccggaacc
      121 gccgacgcca ccgggctctc atagccgtcg cgggagacgg aggagactcc gaagacgtaa
      181 tcgtcgaccc gcacgccctt gagcgcctga acgcaggtgg cgacgtgcct gtcccccacc
      241 gcattgggcg ccgcattggg cgccgccggg cgggccgggc atctgtccag gcccgcatag
      301 tcgactcgca ggctgtgctc ccaattggcg gcgtcggtgc gccgccagcg gacgatatag
      361 gcctcggcgc ccctgaccgg gttccacgcg atattggtat cggtggagac ggcgccctcc
      421 acccgcggct ccggcggttg cggagcgctg gccagcgccg ccagcgccgc cacgttcagt
      481 ttcacgacct tggtcaggta gggaaagtcc atcttgtcga tcgtgtcgcc atattggatc
      541 ccgttctcga ccctcagatc ctggtgctgc cagttgtaat tctcgatggc gacggtgatc
      601 cgaacggcgg ggaagccggc gttgagcatc tcggtatggt cgccgccgcg gccgaagcgg
      661 tcgttgcgcc agatctggcg gacgtcgagt ccgaggctga gatcggcggc gaggtcgtcg
      721 agccagcgcg agatgttgcg cgacggagcg tcattctccc cgccgaggct tcggattctc
      781 gcggcgaggt cctcatggcc ctgccagcgc ggcccctccg agaagacccg gacatgggcg
      841 tcgtcgcaca ccccgtccga gctgcagctg ttgccgatga tgtcgttgtt gaggttcgcg
      901 acgacctccc agccctgcgc tttggcgtaa tcggccatga tcttgccgcc gagcagcccc
      961 tgctcctcgc cggagagcgc cgcatagacg atagtgtagg ggaatttgtg cttcgaaagg
     1021 acccgcgccg cctcgatcac ggcggccgtg ccggacccgt cgtcattggc gccgggcgca
     1081 tcggaggtcg cgttcatcgg atcggtgacc cggctgtcga tatggccgga gatgatcacc
     1141 acccggttcg gatccgcgct cccgcgctgc acggcgaaga cgttggtcac cctggtcggg
     1201 ttcgggaccc ggcggcccgt gacgacatcg gacggtgtga cgacccggag acagcccccg
     1261 caggcccgcg aataacggcc gaactccgcc tcggtccatc tgagcgacgc tccgatgccg
     1321 cgcttcggat cggtctgcga ggaaagggtg tgacgggtgc cgaagctgac cagcttctcg
     1381 acgatcgccc ggagccgctc ctggctgacg gcgtcggcga tctcccgcgg agtggcggcg
     1441 gaagcgggag cggcaaggag aaggacgggc aggagcaggg cgagcttttt catggccttg
     1501 gcccctgaac ctcccggccg gatttcgcaa gcgcgggtaa gccgatgtgc tttacacatg
     1561 taagacgctc tgtcagaccg gccctcgaac atccgggttg gggaactcct tcaaatgcta
     1621 gaactcgccg acgtcagcca cgtctatccg aacggcacgc gcgctctcga ccacgtcacc
     1681 ttgtcgatcc cgcggggcat gtacggcctg ctcgggccca acggcgcggg caagtcgacc
     1741 ctgatgcgca acgtcgcgac gctgcagacc cccaccgagg gcagcatccg cttcggcgac
     1801 atcgacgtca tcgccgagcc cgagaagctg aggcggacgc tcggctacct gccgcaggat
     1861 ttcggggtct atccgcgggt ctcggcctac gacatgctcg atcacatggc ggtgctgaag
     1921 ggcatcgcct ccgccccggc gcgcaagcag acggtcgaag ggctgctcca ccaggtcaac
     1981 ctctgggatg tgcgcaagaa ggcgctggcg ggtttctcgg gcggaatgcg ccagcgcttc
     2041 gggatcgccc aggcgttgat cggcaatccg gagctgatca tcgtcgacga gccgaccgcc
     2101 ggcctcgacc cggaggagcg caaccgcttc ctcaaccttc tcgccgagat cggcgagaat
     2161 gtggtggtga tcctgtccac ccacatcgtc gaggacgtgt ctgacctgtg cccgcggatg
     2221 gcggtgctcg ccggcgggcg gattcagctc gaaggggctc cgctcgagct catccgctcc
     2281 acccgggggc ggatctggat gaaggtgatc gagcggggag agctcgagct ctaccgcgag
     2341 aaatacgagc tgatctccac ccgcctgttc gcggggcgta ccgtcatcca catcctctcc
     2401 gacaccgatc ccggcgacgg cttcgcctcg gtcgagggcg ggctcgagga cgtgtatttc
     2461 acgaccctcg tccaatcccg ccgcgccgcc tgaggagctg aagacaatgt tcctcaagat
     2521 cgctcgattc gagctgcgct accaggtcaa gaacccggtc ttctgggtgg cggcggtcat
     2581 gttcttcctg ctctgcttcg gattcatggc ctccgaggcg gtgagcggcg gcgccagcgg
     2641 caacctgcac cgcaatagcc cggtccagct cacccagatc cagaccacgt tcagcctgtt
     2701 cttcatgttc gtgacgacgg ccttcgtcgc caacatcgtg gttcgcgacg acgagaccgg
     2761 cttcgggccg atcatccggt cgactccgat cacccggctc caatatctcg gcggccgttt
     2821 cgccggagcg gtcgcggcgg caagcctcgc ctatctgttc atcgccctcg gcctctgggt
     2881 cggctcgatc atgccctggg tcgacccgga gaccctcggg ccaaaccgcc tctcttatta
     2941 tctctatgga atcgcggtct tcggggtgcc gaacctgctc gtcaccggag cgatcttctt
     3001 caccgcggca accctgaccc gctcgatgat gtggacctat gtcgcggtcg tggtcttctt
     3061 cctcgcctgg ctcatcctga cgggaatcgg ccaggcgaag cccgagcttc gcgacacgct
     3121 cgccctggtg gagccgttcg gggtcggagc cttcggcaac gcggtcaaat actggaccac
     3181 cgccgagcgc aacatcggcc tcccgccggt cgagggcgcg atccttatga accggctgat
     3241 ctggctggga atcgcctcgg ccttcctcgc cttcgcgatc gcccgctacc ggttcgccga
     3301 accggcgctc tcgaagaagc gccgcaagct tgaagcggcg caggccgcgc cggcacccgt
     3361 tccgtcgccc gctccggcgg ctgcgctccc cgatgccggc ggctcggcgg cctgggcgca
     3421 gctccgggcc cggacccggt tcgagatgaa gcaggtggtc aggagcccgg ctttcttcgt
     3481 cctggtcgcg ctggggatga tcaacaccgt cgccaatttg tggttcgcgg gcgagatgtt
     3541 cggaaccccg acccttccgg tgacccggtg ggtgatcccg atgctggccg gctccttcgg
     3601 catcttctcg atcatcatcg gcatctatta ttcgggcgag ctg
//
LOCUS       DASWNU010000210         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_112288, whole genome shotgun sequence.
ACCESSION   DASWNU010000210 DASWNU010000000
VERSION     DASWNU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112288"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="VGB57_04140"
     CDS             complement(<1..2167)
                     /locus_tag="VGB57_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0130573.1"
                     /translation="MANFRGTAGPDDLTGTSEADLMVGEAGDDVLRGRGGDDDLYGGA
                     GSDELRGGSGDDYLNGDDGDDYLYGGLGNDVLWGGLGNDEIWGGDGDDVINDHGGDDW
                     ADGGNGNDSFYSLDGDDLLVGGAGDDDFTLDRSSPFGSHYVCLDGGDGNDRFNFWVRE
                     SGLIEVAAGPGSDSIEINDLSDRLTVFFDPDGTDTVTLSALMAGSYRSSTIVLYGFAR
                     GDEGGVIVWHDFLGQALSGWDGSSNPFGSAGYARIGSDGWNQILEIDPDGGGDSWVKV
                     MNLGPATGTAFTAHNFDGFSPDGSPAAGQVLTGTPGPDELAGASGADTIIGLAGDDRL
                     YGRAGADSIDAGAGDDRVEGGPGADSIQGGDGDDFLDGGHGDDVLHGGAGNDWLDDSN
                     FVDGGGRGALHGGDGDDTLILFRYFAAPVETHLLSGGDGNDRFSVGLAYGGTLIVDGG
                     AGDDRIRLVAQDRGAGAIVTLGAGNDSLEIDGDTLDPADSALRITDFTAGTAGDRLQI
                     SGLGSLLAPGWEASPLFESGHFGLVPTAGGTLLLIDRDGGGDSFVTLATLDGVTTLAL
                     TAENLGGNAPPVTYFSGGNDQIAGTAGTDLLSGLGGADMFRLQQGGDDAVAGGEGADL
                     FYFGAALTEADHVDGGGGVDTLIVQGNYAGGLKPGAFVTGIENISILGGNNTAFGDPG
                     TNRYDYVLTTNDANFAAGVQARINGAALLAGEDFTFDGSA"
     gene            complement(2397..>2785)
                     /locus_tag="VGB57_04145"
     CDS             complement(2397..>2785)
                     /locus_tag="VGB57_04145"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130574.1"
                     /translation="LKGGALNDLIHGNLGADTLAGNGGADAFRYQNVAESSSGSMDRI
                     LDFTPGTDKIELDRIDANALVAGNQAFSWIGSSAFTGSAGQLRAYEQGGSWFVEGDVN
                     GDGSADLVIALTLQGATPLGAGDFLL"
BASE COUNT          425 a          955 c          943 g          462 t
ORIGIN      
        1 ccgccgagcc gtcgaaggtg aagtcctcgc cggcgagcag ggcggcgccg ttgatccggg
       61 cctgaacgcc ggccgcgaag ttggcgtcat tggtggtcag aacgtaatcg tagcggttgg
      121 tgccggggtc gccgaaagcg gtgttgttgc cgccgaggat cgagatgttc tcgattcccg
      181 tgacgaaggc gcccggcttg agcccgcccg cataattgcc ctggacgatc agagtgtcga
      241 ctccgccgcc gccgtcgaca tggtccgcct cggtgagcgc ggcgccgaaa tagaagaggt
      301 cggcgccctc gccacccgcc acggcgtcgt cgccgccctg ctggagccgg aacatgtcgg
      361 ctccgccaag gcccgagaga agatcggtgc cggcggtgcc cgcgatctgg tcgttgccgc
      421 cgctgaaata ggtgaccggg ggcgcgttgc cgccgagatt ttccgcggtc agagccagag
      481 tcgtgactcc gtcgagcgtg gcgagtgtga cgaagctgtc gccgccgccg tcgcggtcga
      541 tcagcagcag ggtgccgccc gccgtgggaa cgaggccgaa atgacccgac tcgaagaggg
      601 ggctggcctc ccagcccggg gcaagcagcg agccgagccc cgagatttgc agccggtcgc
      661 cggccgttcc cgcggtgaag tcggtgatcc tgagggcgct gtcggcgggg tccagcgtgt
      721 cgccgtcgat ctcgagactg tcgttgccgg cgccgagcgt cacgatcgcg cccgcgcccc
      781 gatcctgcgc gaccagccgg attcggtcgt cgccggcgcc gccgtcgacg atcaacgtgc
      841 cgccataagc cagcccgacg ctgaatcggt cattaccgtc gccgccgctc agaagatgag
      901 tttcgacggg cgcggcgaag tagcggaaca ggatcagggt gtcgtcgccg tcgccgccgt
      961 gaagagcgcc tctgccgccc ccgtcgacga aattggagtc gtcgagccag tcgttgccgg
     1021 ctccgccatg gaggacgtcg tcgccgtggc cgccgtcgag gaaatcgtcg ccgtcgccgc
     1081 cctggatcga gtccgctccg ggcccgcctt cgacacggtc gtcgcccgcg ccggcgtcga
     1141 tcgagtcggc cccggcgcgg ccatagagcc tgtcgtcccc ggccaggcca attatcgtat
     1201 cggcgccgct tgccccggcg agctcgtcgg gaccgggagt gccggtcagc acttggcccg
     1261 cggccgggct tccgtcggga gagaagccgt cgaaattgtg cgccgtgaaa gccgtgccgg
     1321 tggccggacc gagattcatc accttgaccc agctgtcccc gccgccgtcc ggatcgatct
     1381 ccagtatctg gttccagccg tccgagccga tcctggcgta acccgcgctt ccgaacgggt
     1441 tggaggagcc gtcccagccg ctgagagcct ggccgaggaa atcgtgccag acgatcaccc
     1501 cgccctcgtc tccgcgggcg aagccgtaga ggactatggt cgaggaccga taggaaccag
     1561 ccatcaaggc actgagggtg accgtgtcgg tcccgtccgg atcgaagaag accgtcagcc
     1621 ggtcggagag gtcgttgatc tcgatgctgt cgctgcccgg gccggcggcc acctcgatca
     1681 ggccgctctc ccttacccag aagttgaacc ggtcgtttcc gtctccgccg tcgaggcaga
     1741 catagtgcga gccgaacggc gacgatcgat cgagggtgaa atcgtcgtcg ccggcgccgc
     1801 cgacgagcag gtcgtcgccg tcgaggctgt agaagctgtc gttgccgttc ccgccgtccg
     1861 cccagtcgtc gccgccatgg tcgttgatca catcgtcgcc gtcgccgccc cagatctcgt
     1921 cgttgccgag gccgccccag aggacgtcgt tgccgaggcc gccgtacaga tagtcgtcgc
     1981 cgtcgtcgcc gttcagatag tcgtcgccgc tgccgcccct cagctcgtcg gatcccgcgc
     2041 cgccgtacaa atcgtcgtcg ccgcctcttc cgcgcagcac gtcgtctccg gcctcgccca
     2101 ccatgagatc cgcttcggaa gtgccggtaa ggtcgtcagg gcccgcagtg cctcgaaaat
     2161 tcgccatgag atgactcccg ccgtcgatgc tggcggcaca tctgcgcgca aatcagtggc
     2221 ttatccacgg ggcttcagac gcagtcccgt tttgggacga tcgccgggtc gatcctttcc
     2281 gaaaggccgg gatcagagcg ggtgctctac ccgtcatccc ggcgaaagcg ggacctcagg
     2341 cagaatgagg ctctgccggg tgagccgagg tcccggcttc cgccgggacg acggtgctag
     2401 agcaggaagt cccccgctcc gaggggtgtg gctccctgca gagtcagcgc gatgacgagg
     2461 tcggcgctgc cgtcgccgtt cacgtcgccc tcgacgaacc aggagccgcc ctgctcatag
     2521 gctcgaagct ggccggccga gccggtgaag gcgctggagc cgatccagct gaacgcctgg
     2581 ttgcccgcga cgagcgcgtt ggcgtcgatg cgatcaagct cgatcttgtc cgtccccggc
     2641 gtgaagtcga ggatccggtc catcgacccc gagctcgact ccgcgacatt ctggtagcgg
     2701 aacgcgtccg ccccgccatt gcccgcgaga gtgtcggcgc ccaggttgcc atggatgagg
     2761 tcgttcagtg ctccgccctt caagg
//
LOCUS       DASWNU010000211         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_241431, whole genome shotgun sequence.
ACCESSION   DASWNU010000211 DASWNU010000000
VERSION     DASWNU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241431"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VGB57_04150"
     CDS             <1..582
                     /locus_tag="VGB57_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130575.1"
                     /translation="SMWWNREELGNRAGTMLRGRDYSDRRLYLTMANEGGTMRRGLDK
                     LLAALRTPAAGALKWTWVDRRASEHHGSIYHLAALDALRILYPKPYRPGSPLPWLYVG
                     EVPKLSEAAEADKKIPCTAERARRVTFAEVEADPERWEALCVLSPLGQAPEPREWSRN
                     WRLDAGTPSSRRKPGPGPEKSRRVSPRPRLSPG"
     gene            complement(597..1499)
                     /gene="rpoH"
                     /locus_tag="VGB57_04155"
     CDS             complement(597..1499)
                     /gene="rpoH"
                     /locus_tag="VGB57_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY0130576.1"
                     /translation="MASGKSVATIPAMSGEAGLNRYLAEIKKFPILAPEEEYMLAKRW
                     TEHQDTDAAAKLVNSHLRLVAKIAMGYRGYGLPTAELISEGNIGLMQGVKKFEPERGF
                     RLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRRMKNQIEAFEDGDLKPA
                     DVTKIATDLGVSEEDVISMNRRMAMGGDTSLNVSLREEGEGQWQDWLVDTDPLQDERV
                     AEAQESQVRHELLVEAMKALNDREKHILTERRLTDEPKTLEELSQVYDVSRERIRQIE
                     VRAFEKLQKALMGLAGERRLLPAS"
     gene            complement(1557..>2296)
                     /locus_tag="VGB57_04160"
     CDS             complement(1557..>2296)
                     /locus_tag="VGB57_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0130577.1"
                     /translation="PVQAHNEAQDIALDIVFEDDHLLVVDKPAGMVVHPAAGNFDGTM
                     VNALLHHCAGRLSGIGGVARPGIVHRIDKGTSGLLVVAKTDRAHEGLAAQFARHSIDR
                     RYLAVVAGVPNPTAGTIDAPLARSSANRQKMAIVEGNRGKRAVTHYRMLRPLRDSALV
                     ECRLETGRTHQVRVHMTSIGNPLLGDPAYGRTRSAHRELLKKLGFERQALHAAELGFI
                     HPVTSAPLSFKSDAPSDMQQLLSALAV"
BASE COUNT          363 a          771 c          737 g          425 t
ORIGIN      
        1 agcatgtggt ggaaccggga ggagctcggc aaccgcgccg ggacgatgct caggggcagg
       61 gactattcgg accggaggct ctacctcacc atggccaatg agggaggcac gatgcggcgc
      121 ggcctcgaca agctcctcgc cgcccttcgg accccggcgg ccggcgctct caaatggacc
      181 tgggtcgacc gccgcgccag cgagcatcac ggcagcatct accacctcgc cgcgctcgat
      241 gcgcttcgca ttctctaccc caagccatac cggcccggct cgccgctgcc gtggctgtat
      301 gtcggcgagg tgccgaagct gagcgaggct gccgaggccg acaagaagat cccctgcacg
      361 gccgagcgcg cccgccgggt cactttcgcc gaggtcgagg ccgatccgga gcgatgggag
      421 gctttgtgcg tcctctcgcc gctcgggcag gcgccggagc cccgcgaatg gtcgaggaac
      481 tggaggctgg acgctggcac tccgtcatcc cggcgaaagc cgggacctgg gcccgaaaag
      541 tcacggcggg tctccccgag gccccggctt tcgccggggt gacggttgac gcgcgcttac
      601 gaagccggaa gcagccgccg ctcgccggct aggcccatca gcgccttttg aagcttctcg
      661 aacgccctga cctcgatctg gcggatgcgc tcgcggctga cgtcgtagac ctgagagagc
      721 tcctcgagcg tcttgggctc gtcggtcagc cgccgctcgg tgaggatgtg cttctcgcgg
      781 tcgttcaggg ccttcatcgc ctcgaccaga agctcgtgcc ggacctggct ctcctgggcc
      841 tcggcgactc gctcgtcctg aagcgggtcg gtgtcgacca gccagtcctg ccactgccct
      901 tcgccttcct cgcgcagcga gacgttgagc gaggtgtcgc cgcccatcgc catccggcga
      961 ttcatcgaga tgacgtcctc ttcggacact ccgaggtcgg tggcgatctt ggtgacgtcg
     1021 gcgggcttga gatcgccgtc ctcgaacgcc tcgatctggt tcttcatccg cctgaggttg
     1081 aagaagagct tcttctgggc cgcggtggtg cccatcttca caaggctcca cgagcgcagg
     1141 atatattcct ggatcgacgc ccggatccac cacatcgcat aggtggcgag gcggaagccc
     1201 ctttccggct cgaacttctt cacgccctgc atcaggccga tattgccttc cgagatcagc
     1261 tcggccgtcg gcaggccgta gccccgatag cccatggcga tcttcgcgac gaggcggagg
     1321 tgcgagttga ccagcttggc cgcggcatcg gtgtcctgat gctcggtcca gcgcttggcc
     1381 agcatatatt cctcctcggg ggcgaggatc gggaatttct tgatctcggc gagatagcgg
     1441 ttgagacccg cttcgccgct catggcggga atcgtcgcca cgcttttacc gctagccatc
     1501 gtcttcactc actccttggt gtccagctgc ttttgatttt gccgggaccg tcgcccttat
     1561 accgccagcg cgcttaacag ttgctgcata tccgacggag cgtcgctttt gaacgacaag
     1621 ggcgccgatg tcacgggatg gatgaaccca agctccgccg catgcagcgc ctgccgctca
     1681 aaacccagct ttttcaataa ctcacgatgc gccgatcggg tccgtccata ggccgggtcg
     1741 ccgagcaaag ggttgccgat cgaagtcatg tggacccgaa cctggtgagt ccgccccgtt
     1801 tcgagccggc attcgaccag agccgagtcc ctcaagggcc gaagcatccg atagtgggtc
     1861 accgccctct tgccgcgatt cccctcgacg atcgccatct tctggcggtt ggccgaggag
     1921 cgggcgaggg gcgcgtcgat cgttcccgcg gttgggttcg ggacccccgc gacgaccgcc
     1981 agatagcggc ggtcgatgct gtggcgggcg aattgcgccg ccaggccctc atgggcgcgg
     2041 tcggtcttgg cgacgacgag cagccccgaa gtccccttgt cgatgcggtg gacgatcccc
     2101 ggccgggcca ctccgccgat cccggaaaga cgtccggcgc aatggtggag aagggcgttc
     2161 accatcgtcc cgtcgaaatt gcccgccgcc ggatggacca ccattcccgc cggcttgtcg
     2221 accacgagca ggtgatcatc ctcgaagacg atgtcgagtg cgatgtcctg ggcctcatta
     2281 tgggcctgca ccggct
//
LOCUS       DASWNU010000212         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_423446, whole genome shotgun sequence.
ACCESSION   DASWNU010000212 DASWNU010000000
VERSION     DASWNU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423446"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VGB57_04165"
     CDS             <1..431
                     /locus_tag="VGB57_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130578.1"
                     /translation="GAIAPEALAAWDALKDDVPGLGLLAITSPDLLHRGWSASQASRW
                     RKTPPRNGEGDRRPQAGGGGGHSHVERLLAPLSPTAGLVTLLDGAPASLSWLGGVIGH
                     RVSPLGLDRFGQTGTLGDLYREYRLDVDAIVEAAAELFLD"
     gene            491..757
                     /locus_tag="VGB57_04170"
     CDS             491..757
                     /locus_tag="VGB57_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130579.1"
                     /translation="MIGKIASAFIGKRIADRYGTGGRGMIIGALAPMVARRAFGPLGL
                     VLAGGYVAKKVYDGRKRRARTAVCGFGRGGWLRRRPASLYPEQP"
     gene            complement(803..1651)
                     /gene="aguB"
                     /locus_tag="VGB57_04175"
     CDS             complement(803..1651)
                     /gene="aguB"
                     /locus_tag="VGB57_04175"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEY0130580.1"
                     /translation="MSEMKVAALQLAFSDVVEENIAAVSELVRDAAGKGAKLILPPEL
                     FEGHYFCRTQDEGHFTRALPAGEHPVVQAMQKLAAELQVYIPTSFFEAEGHHHYNSLA
                     MIDDGGAVMGVYRKSHIPDGPGYSEKFYFRPGNTGFKTFKTPFGTIGPAVCWDQWYPE
                     TARTLMLEGAEILLFPTAIGTEPHDPDLDTSRMWRRAMIGHAVSNVVPVVAANRIGTE
                     GDQRFYGHSFICDERGDMLAEFGATETGVLTATLDLQQAKKHRAAFGFFRDRRPELYR
                     RLVQDV"
     gene            complement(1648..>2116)
                     /locus_tag="VGB57_04180"
     CDS             complement(1648..>2116)
                     /locus_tag="VGB57_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY0130581.1"
                     /translation="REHLGIERVLWLGEGLLNDHTDGHIDNLARFVGENRLAIPVPSG
                     EDDPNAAVYADAKARAEAFGVDVVPLPSPGLVLRPSASPGTGDGEIIPASYMNFYIGN
                     AAVVVPLYGAPNDDAAVEAIGALFPDRKAVGIRADHVLTGGGSFHCISQQVPR"
BASE COUNT          339 a          692 c          729 g          356 t
ORIGIN      
        1 ccggcgccat tgcgcccgag gcgctcgccg cctgggatgc gctgaaggac gacgtgcccg
       61 gcctcggcct cctcgcaatc acatcccccg atctcctcca ccgcggctgg agcgcctcgc
      121 aagcgagtcg ctggcgcaaa actcctcccc ggaacgggga gggggaccgc cggccgcagg
      181 ccggtggtgg agggggccac tcgcacgtcg agcgcttgct cgccccgctt tcgccgaccg
      241 ccggtctcgt cacccttctg gacggcgcac ccgcctcctt gtcctggctc ggtggcgtga
      301 tcggccaccg ggtcagcccg ctcggcctcg accgtttcgg ccagaccggg acgctcggcg
      361 acctttatcg cgaataccgg ctggacgtgg acgcgatcgt cgaagccgcg gccgagctgt
      421 tcctcgattg agggaacatt cgggcggcct cccgcgctct agcccccgca gcttagacat
      481 ggagttcagt atgattggga agatcgccag cgcattcatc ggcaagcgta tcgcggaccg
      541 ctacggcacc ggcgggcgcg gcatgatcat cggtgcgctc gcgccgatgg tggcgcgacg
      601 cgccttcggg ccgctcggcc tggtgctggc gggcggctat gtcgccaaga aggtctatga
      661 cggccgcaag cggcgcgcgc gcaccgcggt ctgtggcttc gggcggggcg gctggctcag
      721 gcgccgcccc gcttcccttt atcctgagca accatgaggc cgccgagctg aggccggtga
      781 acctcccggt tcaccccgct gattagacat cctgaaccag ccgccggtaa agctcggggc
      841 ggcggtcgcg gaagaagccg aaggcggcgc ggtgcttctt cgcctgctgg agatcaaggg
      901 tggcggtgag aacgcctgtc tccgtcgcgc cgaactccgc cagcatgtcc ccgcgctcgt
      961 cgcagatgaa gctgtggccg tagaagcgct gatcgccctc ggtgccgatg cgattggccg
     1021 cgacgacggg aacgacgttc gagaccgcgt ggccgatcat cgcccgccgc cacatgcggc
     1081 tggtgtcgag atccggatcg tgcggctccg tgccgatagc ggtcgggaag agcaggattt
     1141 cggcgccttc cagcatcagg gtgcgggcgg tctcgggata ccattggtcc cagcagacgg
     1201 cggggccgat cgtgccgaag ggggtcttga aggtcttgaa gccggtattg cccgggcgga
     1261 aatagaattt ctccgaatag cccggcccgt cggggatgtg gctcttccga tagacgccca
     1321 tcaccgcgcc tccgtcgtcg atcatggcga ggctgttata atgatgatgc ccttcggcct
     1381 cgaagaagct ggtggggata tagacctgga gctcggcggc gagcttctgc atcgcctgga
     1441 cgaccgggtg ctcacctgca gggagggccc tggtaaagtg gccttcatcc tgcgtgcggc
     1501 agaaataatg gccttcgaac agctcgggcg gcaggatgag cttggcgccc ttgccggccg
     1561 catcgcggac gagctcggac accgcggcga tattctcctc gaccacgtcc gaaaaggcga
     1621 gctggagggc ggctactttc atttcgctca acggggcacc tgctggctga tgcaatggaa
     1681 gctgccgccg ccggtcagca catgatcggc cctgatccct acggccttcc ggtccgggaa
     1741 cagcgcgccg atcgcttcca cggcggcatc gtcgttcggc gcgccgtaga gcggaaccac
     1801 gacggccgca ttgccgatgt agaaattcat ataggaagcc gggatgattt caccatcgcc
     1861 tgtcccgggc gaagccgagg ggcgaaggac gagaccgggc gagggcaggg ggacgacatc
     1921 aacgccgaaa gcttcggcac gcgctttggc gtcggcatag acggcggcgt tcggatcatc
     1981 ctcgccggac gggacaggga ttgcgaggcg gttctccccc acgaaccggg cgagattgtc
     2041 gatatggccg tccgtatggt cgttgagcag cccctcgccg agccagagca cgcgctcgat
     2101 gccgagatgc tcgcgc
//
LOCUS       DASWNU010000213         4177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_162801, whole genome shotgun sequence.
ACCESSION   DASWNU010000213 DASWNU010000000
VERSION     DASWNU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162801"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..492)
                     /locus_tag="VGB57_04185"
     CDS             complement(22..492)
                     /locus_tag="VGB57_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0130582.1"
                     /translation="MKKMLKSHVFAAFAAAAALAGCDRPPPDGSVRVTDVWVRLPAAK
                     SRPGGAYFRMEAGSEGTRLLGVTSPSVRWIELHETETKAGKTRMKQRKEIEFPSRGEL
                     VFEPGGGHAMLFGINSKVKAGDRIPLTFSFNVAPPITVEAQVLAAGDEAPGDGH"
     gene            630..2558
                     /gene="dnaK"
                     /locus_tag="VGB57_04190"
     CDS             630..2558
                     /gene="dnaK"
                     /locus_tag="VGB57_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0130583.1"
                     /translation="MARVIGIDLGTTNSCVAVMEGGKPKVVENAEGARTTPSIVAFAK
                     DGERLVGQPAKRQAVTNPENTVFAVKRLIGRRFDDPITKKDTELVPYKISRGPNGDAW
                     VNAGAKDYSPSQISAFILQKMKETAEAYLGENVTQAVITVPAYFNDAQRQATKDAGQI
                     AGLEVLRIINEPTAAALAYGLDKQDGKTIAVYDLGGGTFDISVLEIGDGVFEVKSTNG
                     DTFLGGEDFDAKIVDYLADEFKKQEGIDLRGDRLALQRLKEGAERAKIELSSAQTTEV
                     NLPFITADQNGPKHLVKTITRSDLEKLVEDLIKRTLEPCKKAIADAGVKSADIAEVVL
                     VGGMTRMPRVRQIVQEFFGKEPHTGVNPDEVVAMGAAIQAGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRMIDRNTTIPTKKSQTYSTAEDNQNAVTIRVFQGEREMAADNKMLG
                     QFDLVGIPPAPRGVPQVEVTFDIDANGIVNVSAKDKGTGKEQQIRIQASGGLADSDIE
                     KMVREAEQFAEEDKKRREGAEAKNSAEGLAHSTERQLAEHGDKVDEALKTEIQAAIDE
                     TRAAVQSNDVEAMKSKTAALSQVAMKLGQAIYEKEQAGAAGGASDDSGASESAQAGGN
                     EEEVVDAEFSEVDDENKG"
     gene            2670..3788
                     /gene="dnaJ"
                     /locus_tag="VGB57_04195"
     CDS             2670..3788
                     /gene="dnaJ"
                     /locus_tag="VGB57_04195"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0130584.1"
                     /translation="MTETDYYELLQVERGADGQTLKSAYRRLAMECHPDRNPGCKDSE
                     ARFKAINEAYDCLKDPQKRAAYDRFGHAAFRNGGMGGAGAQDFGSFSDIFENIFGEFM
                     NGQQADRRSNALRGSDLRYDLEISLEDAFAGKQVELRIDTTAKCEPCDGSGAKTGTSA
                     RPCTMCHGRGQVRAQQGFFVVERTCPSCRGVGQVIADPCEKCRGEGRVERQRSLEVRI
                     PAGVDEGTRIRLAGEGEAGVRGGPNGDLYIFIHLARHSFFQRQGTTLFVRCPVSITTA
                     ALGGTIEVPGLDRQKHSIQIPAGIQSGKQLRQRGAGMPVVNGRGHGDMVVQVEVETPT
                     KLSSRQKELLEEFRKTETGSECPASQSFFGKLKAMFDG"
     gene            complement(3798..>4177)
                     /locus_tag="VGB57_04200"
     CDS             complement(3798..>4177)
                     /locus_tag="VGB57_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130585.1"
                     /translation="AEAPAAIDAQAALRKRDSVNKPPRVILVTDRHCFSSCLMAARLF
                     RDLGAMHVGQETRANTRYSNAITTDLPSGLSNFSSLQAYWTYVPMRLGPYTPELILAV
                     DLADDRAVEAEVGKLVASPRKGR"
BASE COUNT          801 a         1425 c         1353 g          598 t
ORIGIN      
        1 aatccggggg aggatttcgg ctcaatgccc atcgcccggc gcctcgtccc ccgccgccag
       61 cacctgcgcc tccacagtga tcggcggggc gacgttgaac gagaaggtga gcgggatgcg
      121 gtcgcccgcc ttcaccttcg agttgatccc gaacagcatg gcgtggccgc cgccgggctc
      181 gaagacgagc tcgccgcggc tcgggaactc gatctccttg cgctgtttca tccgggtctt
      241 gccggccttc gtctcggtct cgtgaagctc gatccagcgg accgacgggc tcgtcacgcc
      301 cagcagcctc gtgccctcgc tccccgcctc catccggaaa taggcgccgc ccgggcggct
      361 cttcgccgcc ggcagcctca cccagacgtc cgtcacgcgc accgatccgt cgggcggcgg
      421 ccgatcgcag cccgccagcg ccgccgccgc ggcgaaggcg gcaaaaacgt ggcttttgag
      481 catcttcttc atttccgcgg ctccctagcg ccggccgcac ttcttgtcgc gtcggaatcc
      541 acccctatat cgctcccgaa acactccgcg tgcctcgact tgaaggacgc aggggtgcgg
      601 taaacttttg gggcacaaga ggaataagca tggctagagt catcggaatc gatctcggca
      661 ccaccaacag ctgcgtcgcc gtcatggaag gcggcaagcc caaggtggtc gagaatgcgg
      721 aaggcgcgcg taccaccccc tcgatcgtcg ctttcgccaa ggacggcgag cggctggtcg
      781 gccagccggc caagcgccag gcggtcacga accccgagaa caccgtcttc gcggtgaagc
      841 gcctgatcgg ccgtcgcttc gacgatccga tcaccaagaa ggacaccgag ctggtcccct
      901 acaagatcag ccgcggtccc aacggcgacg cgtgggtcaa cgccggcgcc aaggattaca
      961 gcccgtccca gatttccgcc ttcatcctcc agaagatgaa ggagaccgcc gaggcctatc
     1021 tcggcgagaa cgtcacccag gcggtgatca ccgttcccgc ctacttcaac gacgcccagc
     1081 gccaggcgac caaggacgcc ggccagatcg ccggcctcga ggtgcttcgg atcatcaacg
     1141 agccgacggc cgcggcgctc gcctacggcc tcgacaagca ggacggaaag acgatcgcgg
     1201 tctacgatct cggcggcgga acgttcgaca tctcggtgct cgagatcggc gacggcgtgt
     1261 tcgaggtgaa gtcgaccaac ggcgacacct tcctcggcgg cgaggatttc gacgccaaga
     1321 tcgtcgatta cctggcggac gagttcaaga agcaggaggg catcgacctt cgcggcgacc
     1381 gcctcgccct ccagcgcctc aaggaaggcg cggagcgggc gaaaatcgag ctcagctcgg
     1441 cccagaccac cgaggtcaac ctgcccttca tcacggcgga ccagaacggg cccaagcatc
     1501 tcgtcaagac gatcacccgc tcggatctcg agaagctggt cgaggacctg atcaagcgga
     1561 cgctcgaacc ctgcaagaag gcgatcgccg acgccggcgt caagtcggcc gatatcgccg
     1621 aggtcgtcct cgtcggcgga atgacccgca tgccgcgggt ccgccagatc gttcaggaat
     1681 tcttcggcaa ggagccccac acgggcgtca acccggacga ggtcgtcgcc atgggcgccg
     1741 ccatccaggc gggcgttctc cagggcgacg tcaaggacgt cctcctgctc gacgtcactc
     1801 cgctctcgct cggaatcgag accctgggcg gcgtcttcac ccggatgatc gaccgcaaca
     1861 cgaccatccc gaccaagaag tcgcagacct actcgaccgc cgaggacaat cagaacgcgg
     1921 tcaccatccg ggtcttccag ggcgagcgcg agatggccgc ggacaacaag atgctcggcc
     1981 agttcgatct ggtcggaatc ccgccggcgc cgcgcggagt gcctcaggtc gaggtcacgt
     2041 tcgacatcga cgccaacggc atcgtcaacg tctcggccaa ggacaagggc accggcaagg
     2101 agcagcagat ccggatccag gcctcgggcg gcctcgccga ttccgacatc gagaagatgg
     2161 tccgcgaggc ggagcaattc gccgaggagg acaagaagcg ccgtgagggc gccgaggcga
     2221 agaacagcgc cgagggcctc gcccacagca ccgaaaggca gctggcggag catggcgaca
     2281 aggtcgacga ggccctgaag accgagatcc aggcggcgat cgacgagact agggcggcgg
     2341 tccagagcaa cgacgtcgag gcgatgaagt cgaagaccgc ggcgctcagc caggtcgcga
     2401 tgaagctcgg ccaggcgatc tacgagaagg agcaggccgg agcggccggc ggcgcttccg
     2461 acgattccgg tgcctccgag agcgcgcagg cgggcggcaa cgaggaagaa gtcgtcgacg
     2521 ccgaattctc cgaggtcgac gacgaaaaca agggctgacg atgagcagcg ccgtcatccc
     2581 ggcgaaagcc gggacctcag cgaggatagg ctcagccctt ctccctgggg ccccggcttc
     2641 cgccggggtg acggcctgtg ggggccgtca tgactgaaac cgattattac gagctgctcc
     2701 aggtcgagcg cggcgccgac gggcagaccc tcaagtccgc ctatcgccga ctcgccatgg
     2761 aatgccatcc cgaccgcaat cctggctgca aggacagtga ggcgcggttc aaggcgatca
     2821 acgaagccta tgactgcctg aaggacccgc agaagcgcgc cgcctacgac cgcttcggtc
     2881 acgccgcctt ccgcaacggc ggcatgggcg gggcgggggc ccaggatttc gggtcgttca
     2941 gcgacatctt cgagaacatc ttcggcgaat tcatgaacgg ccagcaggcc gaccgccgct
     3001 ccaacgccct tcgcggctcg gaccttcgct acgatctcga gatcagcctc gaggatgcct
     3061 tcgccggcaa gcaggtcgag ctcaggatcg acaccaccgc caagtgcgag ccctgcgacg
     3121 gctcgggcgc caagaccggg acgagcgccc ggccctgtac catgtgccac ggccgcggtc
     3181 aggtccgcgc ccaacagggc ttcttcgtgg tcgagcgcac ctgcccgagc tgccgcggag
     3241 tcggccaggt gatcgccgat ccgtgcgaga aatgccgggg cgaggggcgg gtcgagcgcc
     3301 agcgctcgct cgaggtccga atcccagccg gagtcgacga gggcacaagg atacgcctcg
     3361 ccggcgaagg ggaggcggga gtccgcggcg gccccaacgg cgatctctac atcttcatcc
     3421 acctggcccg gcactcattc ttccagcgcc aggggacgac cctgttcgtt cggtgcccgg
     3481 tcagcatcac caccgccgcg ctcggcggca ccatcgaggt gcccgggctc gaccggcaga
     3541 agcattcgat ccagatcccg gccggaatcc agtccggcaa gcagctccgg cagcgcggcg
     3601 ccggaatgcc ggtcgtcaac ggccgcggcc atggcgacat ggtcgtccag gtcgaggtcg
     3661 agaccccgac gaagctgagc agtcgccaga aggagctgct cgaggaattc cgaaagaccg
     3721 agaccggcag cgaatgcccc gccagccaaa gcttcttcgg caaattgaag gcgatgttcg
     3781 acggctgagc tgcctgctca ccttcccttt cgcggagacg cgaccagctt gcccacctcc
     3841 gcctccaccg cgcgatcgtc tgccaggtcc acggcgagga tgagctccgg cgtgtacggc
     3901 ccgagccgca tcggcacgta ggtccaatag gcctgaaggc tgctgaagtt cgacaggccc
     3961 gagggcaaat cggtggtgat cgcgttcgaa tagcgggtgt tggcacgagt ctcctgccca
     4021 acgtgcatgg ctccgaggtc gcggaacagc cgcgccgcca taaggcagga gctgaagcag
     4081 tgacgatcgg tcaccagtat cacccttgga ggcttattca cggaatcgcg tttcctcagc
     4141 gccgcctgcg cgtcgatggc cgcgggcgct tcggcga
//
LOCUS       DASWNU010000214         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_318725, whole genome shotgun sequence.
ACCESSION   DASWNU010000214 DASWNU010000000
VERSION     DASWNU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318725"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2322)
                     /locus_tag="VGB57_04205"
     CDS             complement(<1..2322)
                     /locus_tag="VGB57_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0130586.1"
                     /translation="MTDIHKILSAKKPMTLAGAPAGFLPWLAADLARAAKGRAVFVAP
                     DEAAMRHLQDAATYFAPELEVLAFPAWDCLPYDRASPSLRASSERLATLHALQRKADK
                     PQLLVTTVNAATQRTLTPFRVRQLVARLAPGERIDMAKLSALLSANGYHRTDTVQDAG
                     EYAVRGGIVDLFPAGENEGLRLDFFGDEIESVRRFDPATQRTSGDERGGFTLLPASET
                     LLDEDSVKRFRSHYRERFGATATGDPIYQAVSEGRRLAGIDHWLPLFEERLDTLFEHL
                     ADDDIVVRDAHDAGAADARFEAIADYYENRKRAQSSDPGSYRPLEPETLYLAREEWEG
                     LIGERPLHLATPFHEPESAAVIDFEVDPARDFAPERSQNVNVYEAVVEHVSALRRGKK
                     KVVLASYSAGARERLKGLLADHGLKKAVEVDTWQEAQGASGIALAVLPLDHGFTTADV
                     ALLTEQDMLGDRLVRRRKRRKSADAFMSELATLSPGDLVVHADHGIGRYEGLTQIPVG
                     TSPHDCVALEYAGGDKLYVPVENIDILSRYGSESDGVALDRLGGEAWQRRKSRMKERI
                     REIAGELIKTAAERALRPGAVVEPDTSYAAFADRFPYEETEDQDRAISDVFDDLAAGR
                     PMDRLVCGDVGFGKTEVALRAAFTAAMAGHQVALVCPTTLLARQHYNTFCERFRGFPI
                     EIGRLSRLVTPKESKETKEGLKAGKVDIVIGTHALLAKTVEFKNLGLVIVDEEQRFGV
                     THKERLKAMRANVHVLTLTATPIPRTLQMAMSGL"
     gene            complement(2323..2589)
                     /locus_tag="VGB57_04210"
     CDS             complement(2323..2589)
                     /locus_tag="VGB57_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY0130587.1"
                     /translation="MQPSDRLKRLRFRAWHRGVREADFMIGGFFDAHGESWGESEMAW
                     FEALLEEQDADIMAWAIGTAEPPERYRGETMRRLQQLEYIKHPE"
     gene            complement(2741..>3163)
                     /locus_tag="VGB57_04215"
     CDS             complement(2741..>3163)
                     /locus_tag="VGB57_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0130588.1"
                     /translation="PTTGRPAWSVTSLFLALAMVEIVDVVFAVDSVPAIFAITSDPFI
                     VYTSNIFAILGLRALYFALAAMVHRFHYLKYALAVLLVFIGSKIFVADALGLAKIPPV
                     VSLSVTFAILAAGVGWSLWKTRGSPVIPAKAGIHEHGP"
BASE COUNT          502 a         1020 c         1117 g          524 t
ORIGIN      
        1 aagtccagac atcgccatct ggagggtgcg cgggatcggg gtcgcggtca gggtgaggac
       61 gtggacgttc gcccgcatcg ccttgaggcg ctccttgtgc gtgaccccga agcgctgctc
      121 ctcgtcgacg atgacgaggc ccagattctt gaattcgacc gtcttggcga gaagggcgtg
      181 ggtgccgatg acgatgtcga ccttgcccgc tttcagccct tccttggtct ccttcgattc
      241 cttcggcgtg acgaggcgcg aaagccggcc gatctcgatc gggaagcccc ggaagcgctc
      301 gcagaaggtg ttgtaatgct ggcgggcaag gagcgtggtc gggcagacca gggccacttg
      361 atgccccgcc atcgccgccg tgaaggcggc gcgaagggcg acctcggtct tgccgaagcc
      421 gacgtcgccg cagaccagcc ggtccatcgg ccggccggcg gcgagatcgt cgaagacgtc
      481 ggagatggcg cggtcctggt cctccgtctc ctcgtagggg aatcggtcgg cgaaggcggc
      541 gtagctggtg tcgggctcga ccaccgctcc gggccgaagc gcccgttcgg ccgccgtctt
      601 gatcagctcg cccgcgatct cgcggatccg ctctttcatc cgcgacttgc gccgctgcca
      661 ggcctcgccg ccgagcctgt cgagcgccac tccgtcgctt tcgctgccgt agcgcgagag
      721 gatgtcgata ttctcgaccg gaacgtagag cttgtcgccg ccggcatatt ccagcgcgac
      781 gcaatcgtgc gggctggtgc cgaccgggat ctgcgtcagt ccctcgtagc ggccgatgcc
      841 gtggtcggcg tggacgacga ggtcgccggg gctgagcgtc gcaagctcgc tcatgaaggc
      901 gtcggccgac ttgcgccgct tgcggcggcg gacgaggcgg tcgccgagca tgtcctgctc
      961 ggtgagcaaa gcgacgtcgg cggtggtgaa gccgtggtcg agaggaagaa cggccagggc
     1021 gattcccgaa gcgccctgcg cctcctgcca ggtgtcgacc tcgacggcct tcttgaggcc
     1081 atggtcggcg agcagcccct tgagccgctc gcgcgcgccg gccgagtagc tcgcgagcac
     1141 caccttcttc ttgccccggc ggagcgccga gacatgctcg accaccgcct cgtagacatt
     1201 gacgttctgc gaccgctccg gagcgaagtc gcgggccgga tcgacctcga agtcgataac
     1261 cgccgcgctt tcaggctcgt ggaagggggt ggccaaatgg agcgggcgtt cgccgatcag
     1321 cccctcccat tcctcgcgcg ccaggtagag cgtttccggc tcgagcggcc gatagctgcc
     1381 cggatcggac gattgggcgc gcttcctgtt ctcgtagtaa tcggcgatcg cctcgaagcg
     1441 cgcgtcggcg gcgcccgcgt catgggcgtc gcgaacgacg atatcatcgt cggcgagatg
     1501 ctcgaagagg gtgtcgagcc gctcctcgaa caggggaagc caatggtcga tcccggccag
     1561 tcgccgcccc tcggacaccg cctggtagat cggatcgccg gtcgcggtgg cgccgaagcg
     1621 ctcgcgatag tgggagcgga agcgcttgac gctgtcctcg tcgagcagcg tttcggaggc
     1681 cggcagcagg gtgaagccgc ctcgctcgtc gccgctggtg cgctgggtcg ccgggtcgaa
     1741 gcggcgcacg ctctcgatct cgtcgccgaa gaagtcgagc ctcagcccct cattctcgcc
     1801 cgccgggaac aggtcgacga tcccgccgcg caccgcatat tctcccgcgt cctggacggt
     1861 gtcggtccgg tgatagccgt tggcgctgag cagggccgag agcttggcca tgtcgatccg
     1921 ctcgcccggc gccagcctcg ccaccagctg gcggacccgg aagggcgtca gggtgcgctg
     1981 ggtcgccgcg ttgacggtgg tgacgaggag ctggggcttg tcggccttgc gctgaagcgc
     2041 gtgcagggtc gcaagcctct cggaggaggc gcgcaaggag ggagaagccc ggtcgtaggg
     2101 caggcagtcc caggccggaa aggcgagcac ttccagctcc ggcgcgaaat aggtcgcggc
     2161 gtcctgcaaa tgccgcatcg ccgcctcgtc cggcgcgacg aacacggccc ggcctttcgc
     2221 ggcacgcgcg agatcggcgg cgagccacgg caggaagccc gcgggcgcgc cggccagcgt
     2281 catcggcttt tttgccgaga ggatcttgtg gatgtcggtc acttattcgg ggtgcttgat
     2341 atattcgagt tgctggagcc gccgcatcgt ctctccccgg tagcgctccg gcggctccgc
     2401 ggttccgatg gcccaggcca tgatgtcggc gtcctgctcc tcgagcagag cctcgaacca
     2461 ggccatttcg gactcgcccc aggactcgcc gtgggcatcg aagaagccgc cgatcatgaa
     2521 atcggcttcc ctcacgcccc gatgccaggc gcggaagcga agtcgtttga ggcggtcgga
     2581 gggctgcatt gcgattccca agcggaaaag gccggcacgc tttgggcgtg ccggctcgaa
     2641 ttgccgccga tctagggatt ggcggcgggc ctgtgaagct ctggatcgtc atcccggact
     2701 cgatccggga tccatgagca cggagggctg cgggttttcg ctagggtccg tgttcatgga
     2761 ttccggcctt cgccggaatg acgggtgagc cgcgggtctt ccacagcgac cagccgacgc
     2821 cggccgccag gatcgcgaag gtgaccgaca gcgagacgac cggcggtatc ttcgccagcc
     2881 cgagcgcgtc ggccacgaaa atcttggagc cgatgaacac cagaagcacg gccagcgcat
     2941 atttgaggta gtggaagcgg tggaccatcg ccgccagcgc gaaatagagc gcccgaaggc
     3001 cgaggatggc gaagatgttc gacgtgtaga cgatgaaggg atcggacgtg atcgcgaaga
     3061 tcgccggcac cgaatccacg gcgaagacga cgtctacgat ctcgaccatc gccagcgcca
     3121 ggaagagcga cgtgacggac caggcgggcc ggcccgtcgt cgg
//
LOCUS       DASWNU010000215         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_162840, whole genome shotgun sequence.
ACCESSION   DASWNU010000215 DASWNU010000000
VERSION     DASWNU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162840"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VGB57_04220"
     CDS             <1..1501
                     /locus_tag="VGB57_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130589.1"
                     /translation="ARFAWTLILLAILLILVFLFLGLWPLAILAALGLAYAIRALLRT
                     AREPVGIGADTPAAGSVGTMPVSPDFTLADPFAPGTPAAPTIRPPGFGSDSPELARYK
                     LALEPLFAEDRATAAIAPVRASAPIDIARFAGDLLAAAHPRKIIPAKFETLIRLPGRI
                     REEQREFFAPVMAYPVFDTPMYRHLLGLSDEHFVPNLQYVAQNSISVLETNQPFIEAY
                     MLGLNHEFCREMLWREYPTDQRATCFRQFWDVSSFLDISGKDPELLRDDLRDIPPIHL
                     WPRDSKLGENDHRDRGGPAENELVLVIRGDLLKRYPNAVIYAQRAQWQTTDGVIDNTL
                     ERRLVQLTPEQEANPPEGLLKTPIYEARADPDIYFFGFDLTAPVARGEKGDDPGDDPG
                     WFFVIKERPGEARFGFDVERGSTDTAVWSDIAWSDVTVESGTSFLRASQSHLLSEPAA
                     TDERHEQWLDDSQVPWTVGTNSADLAYILYQLPVMVAVHAADMLPRSPS"
     gene            1498..>4002
                     /locus_tag="VGB57_04225"
     CDS             1498..>4002
                     /locus_tag="VGB57_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130590.1"
                     /translation="MSRTPEFTAALAAFEAAGQAAEKARARAYEARQAQQKADRAKAG
                     SLRDTGAGPAAELNSKLQGLQAKALHERAGFDQASADHFEALAALGPLLDPRERAGEL
                     DDALPILLMPLRIEARFAPDADELWIRAFPDSWAVDSFEPMLSETETLNAQAFWADFW
                     AAGGSTEMQMAAWRNLVASHGAGRATWIATRYRPVNEADRPGDAPANTLTMAVDGERP
                     GEAEAEAIRAYWQSVWREPDSEAARAAAQAALEGTLGAAGAAQVAANPPRRFADPFTA
                     APRNSVTLRLVWIEWRALAEGETKAASWTDAARTDMLPERLVVTLQAGKASRSEIGAI
                     IPPVVTLGFDPAASDSDQFAQVDGELQMPESIAWIFDFDKAVAIGLGLRIALTPAERS
                     DGFDRILVCGLSLRDDSGASRLKLETLFAHHGASATGIALLPQGVPTNNSEIDSGWSS
                     LDDVEGSFERLFGGGSPALDEPDPDLRLDGQWLAHWLGLDAEAVAALPNASGRDQLEA
                     RAMNRVLWPATIGYSLDSLLDGVLPDGAQATTRRYFETTVLARDCVPQIRIGKQPYGI
                     LPASAWSRHRWFGRKRGEGDQAVYLAINSFLAQRLGDAGFLNALFALLRIADSKWREL
                     VPGTANMARPGDLHQTLLDILGLAPNSLEFHRRTADSMEHFYNLMRAQGLDGLFKALI
                     ASAKNEAALKLLRDMGYEGVERPKILRFIFNGRQEAIGKLLVDDVEPSESDQIRFYTD
                     DDRNYLRWLVDSGRDSLEALRVQKGFSGDEVPTALLYHLARHALQLEYAEIGLRLDAA
                     LPNPTLSGAARRAARSDSPFLHIADADSGSESRYFHL"
BASE COUNT          641 a         1423 c         1350 g          588 t
ORIGIN      
        1 tgccaggttc gcctggacgc tcatcctgct cgccatattg ctgatcctgg tgtttctgtt
       61 cctcgggctg tggccgctgg cgatcctcgc cgccctgggg ctcgcctatg cgatccgggc
      121 gctgctgcgc accgcgcgcg agccggtcgg gatcggcgcg gatacgccgg cggcgggaag
      181 cgtggggacg atgccggtca gccccgattt caccctcgcc gatccgttcg ccccgggaac
      241 tccggcggcg cccaccatcc gcccgccggg cttcggcagc gacagtcccg agctcgcccg
      301 ctacaagctg gcgctcgagc cgctgttcgc cgaggatcgg gcgaccgccg cgatcgcgcc
      361 ggtgcgggcc tcggcaccga tcgacatagc ccgctttgcc ggggatctcc tggcggcggc
      421 ccacccgcgc aagatcatcc cggccaagtt cgaaaccctg atccggctgc cggggcggat
      481 ccgggaggag cagcgcgaat tcttcgcgcc ggtcatggcc tatccggtgt tcgacacgcc
      541 gatgtaccgc cacctgctcg gcctgtcgga cgagcatttc gtgcccaacc tgcaatatgt
      601 ggcgcagaat tcgatcagcg tcctggaaac caaccagccc ttcatcgaag cctatatgct
      661 cgggctgaac cacgaattct gccgcgagat gctgtggcgc gaatatccga ccgatcagcg
      721 ggcgacctgc ttccgccagt tctgggacgt cagcagcttt ctcgacataa gcggcaagga
      781 tcccgagctg ctgcgcgacg atctgcggga cattcccccc atccacctgt ggccgaggga
      841 ctcgaagctg ggcgaaaacg accatcgcga ccgcggcggc cccgccgaaa acgagcttgt
      901 cctcgtcatc cggggcgacc tgctcaagcg ctatcccaac gcggtgatct acgcgcagcg
      961 ggcgcagtgg cagacgaccg acggagtcat cgacaacacg ctcgagcggc ggctcgtcca
     1021 gctcacgccc gagcaggagg ccaatccccc ggaaggtctg ctcaagacgc cgatctacga
     1081 ggctcgggcc gatccggaca tctacttctt cggcttcgac ctgaccgcac ccgtggcccg
     1141 gggcgagaag ggcgacgatc ccggggacga tccgggctgg ttcttcgtga tcaaggagcg
     1201 gcccggcgaa gcgcgcttcg gcttcgacgt cgagcggggc agcaccgaca ccgccgtgtg
     1261 gagcgacatc gcctggagcg acgtgacggt cgaaagcggc accagcttcc tgcgcgcgag
     1321 ccagagccac ctgcttagcg agcccgcggc cacggacgag cggcacgagc agtggctcga
     1381 cgacagccag gtgccgtgga ccgtgggcac gaactcggcc gacctcgcct atatcctgta
     1441 ccagctgccg gtgatggtgg cggtccatgc cgcggacatg ctgccgagga gcccgtcatg
     1501 agccgcacgc ccgaattcac cgccgctctg gcggcgttcg aggccgccgg gcaggcggcc
     1561 gagaaggcgc gcgccagggc ctacgaggcg cgccaggcgc agcagaaggc ggaccgggcc
     1621 aaggcgggtt cgctgcgcga caccggcgcc ggcccggccg ccgagctgaa cagcaagctg
     1681 caggggctgc aagcgaaggc gctgcatgaa cgtgccggct tcgaccaggc cagcgccgac
     1741 catttcgagg cgctggcggc gctcggaccg ctgctcgacc cgcgcgagcg cgccggcgag
     1801 ctcgacgacg ccctgccgat cctgctgatg ccgctcagga tcgaggcgcg tttcgcgccc
     1861 gatgcggacg aattgtggat ccgcgctttc cccgacagct gggccgtcga cagcttcgag
     1921 ccgatgctca gcgaaaccga gacgctcaac gcgcaggcct tctgggccga tttctgggcc
     1981 gccggcggca gcaccgagat gcagatggcg gcatggcgca acctcgtcgc cagccacggc
     2041 gcgggccggg cgacctggat cgccacccgc taccggccgg tgaacgaagc ggacaggccg
     2101 ggcgacgcgc cggcgaacac tctgaccatg gccgtcgacg gcgaacggcc gggcgaggcc
     2161 gaagccgaag ccataagggc ttactggcag tccgtctgga gggagccgga cagcgaggcc
     2221 gcccgggccg ccgcccaggc cgccctggaa gggacgctcg gcgcggccgg cgcggcgcag
     2281 gtagcggcca atccgcctcg gcgcttcgcc gatcccttca ccgccgctcc gcgcaacagc
     2341 gtgactctca ggctcgtctg gatcgagtgg cgcgccctgg cggagggcga gacgaaggcg
     2401 gcaagctgga ccgacgcggc ccgcaccgac atgctgccgg agcggctggt cgtgaccctc
     2461 caggccggca aggcgagccg gtcggagatc ggagccatca tcccgccggt ggtgacgctc
     2521 ggcttcgatc ccgcggcgtc cgactccgac cagttcgccc aggtcgacgg cgagctccag
     2581 atgccggagt cgatcgcctg gatcttcgac ttcgacaaag cggtggcgat cggcctcggg
     2641 ctgcgcatcg cactgacccc ggcggagcgc agcgacggct tcgaccggat cctggtctgc
     2701 ggactgtccc tgcgcgacga ttccggcgcc tcccgcctga agctcgaaac cctgttcgcg
     2761 caccatggcg ccagcgcgac cgggatcgcg ctgctcccgc agggcgtgcc gaccaacaat
     2821 agcgagatcg attcgggctg gtcgtcgctc gacgacgtcg agggaagctt cgagcggctg
     2881 ttcggcggcg gatcgcctgc gctggacgaa cccgatcccg atttgcggct cgacgggcag
     2941 tggctcgccc actggctcgg gctggatgcc gaagccgtcg cggcgcttcc caacgcctcc
     3001 ggtcgcgacc agctcgaggc gcgcgcgatg aaccgggtcc tgtggcccgc gaccatcggc
     3061 tattcgctgg acagcctgct cgacggggtg ctgcccgacg gggcgcaggc gacgacccgg
     3121 cgctatttcg agacgacggt gctggcgcgc gactgcgtgc cgcagatcag gatcggcaag
     3181 cagccctacg gcatccttcc ggccagcgcc tggtcccggc accgctggtt cggccgcaag
     3241 cggggcgaag gcgatcaagc ggtctacctc gccatcaaca gcttcctcgc gcagcggctg
     3301 ggagatgcgg gattcctcaa cgcgctcttc gcgctgcttc ggatcgcgga ttcgaagtgg
     3361 cgcgaactcg tccccgggac cgcgaacatg gcgcgcccgg gcgatcttca ccagaccctg
     3421 ctcgacatcc tcggcctcgc gcccaactcg ctcgaattcc accgccgcac ggccgacagc
     3481 atggagcatt tctacaacct catgcgcgcc cagggcctcg acgggctttt caaggccctg
     3541 atcgcctcgg cgaagaacga ggccgcgctg aagctgcttc gcgacatggg ctatgagggc
     3601 gtcgagcgtc ccaaaatcct ccgcttcatc ttcaacggcc gccaggaagc gatcggcaag
     3661 ctgctggtcg acgatgtcga gccgagcgag agcgaccaga tccgcttcta tacggacgac
     3721 gatcgcaact acctgcgctg gctggtcgac tcgggccgcg attcgctcga ggcgctgcgc
     3781 gtgcagaagg ggttcagcgg cgacgaggtg cctaccgccc tcctctacca tctcgccagg
     3841 cacgcgctgc agctcgaata tgcggagatc ggccttcggc tcgatgccgc gctgccgaat
     3901 ccgacgctga gcggagccgc ccgccgggcg gcgcgcagcg actctccctt cctgcacatc
     3961 gcggacgccg attctggcag cgagagccgc tacttccatc tc
//
LOCUS       DASWNU010000216         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_241592, whole genome shotgun sequence.
ACCESSION   DASWNU010000216 DASWNU010000000
VERSION     DASWNU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241592"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1170
                     /locus_tag="VGB57_04230"
     CDS             67..1170
                     /locus_tag="VGB57_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0130591.1"
                     /translation="MDPQSPPPRPRPLLPGEPLPWFRAKAIGGSDNYVFDTAAGRYML
                     LLFFGRAADPGAAEALACVARHRPLFDDIGACFFGVTSDPEDEAGGRVAQQLPGIRFF
                     LDSSGGLAALFGAEPAGKPERRHWLLVDPMLRAVGAFPLEAGEAAVAALAKAIAHMPL
                     PDWAPVLMAPNILEPALCERLVAHHRETGGEPSGFMREVDGKTVLITDPHHKMRRDRE
                     IGDESLCGVLRARILARLVPMVKRTFQFEATRMERYIVGAYPAGEGHFRPHRDNTTRG
                     TAHRRFAVTINLNAGDYEGGDLRFPEFGQRTYRAPTGGAVVFSCSLLHEATPVTSGTR
                     YAFLPFLYDDAAARQREENNPHLGEGIGVYKSG"
     gene            complement(1270..1713)
                     /locus_tag="VGB57_04235"
     CDS             complement(1270..1713)
                     /locus_tag="VGB57_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0130592.1"
                     /translation="MKVTEFMSQDVETVSPDQPIQEAAQFMLRADAGAMPVSDGDKLI
                     GMVTDRDIAVRGVAEGLGPDTPVRDVMTEDLIYCFDDEEVEAVAIKMSDAQVRRMPVL
                     SRDGENLVGIVSLGDISRSDQGEAASVALDGITDPGGEHNQSTEG"
     gene            complement(1831..2607)
                     /locus_tag="VGB57_04240"
     CDS             complement(1831..2607)
                     /locus_tag="VGB57_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEY0130593.1"
                     /translation="MKLRQALPLFALALPGCAASAPAEPPVDGYRIVREYPHDPAAFT
                     QGLVYLDGKLYESTGLNRQSTVREVRLEDGKVLRSISIPPQYFGEGLVNWGSDLLSLT
                     WQDGVGFRWNRADLRPKGQFRYSGEGWGLTQDGKHIILSDGTSNLRFFDPATMQEKKR
                     VSVTYKGQPVERLNELEWVKGEIFANIWMGRRIARIDPKSGAVKGWIDLSQLAVENAS
                     GDPDAVLNGIAYDAKGDRLFVTGKNWAKLFEIDIVPRPKD"
BASE COUNT          384 a          913 c          923 g          470 t
ORIGIN      
        1 ccccagcctt cgctggggcg acgggatccg cttccgcgcc tgcgtctagc agggcataag
       61 gcggcaatgg atccgcaatc ccctccgccc cgtccgcgcc cgctgcttcc gggcgagcct
      121 ctaccctggt tccgggccaa ggcgatcggc ggaagcgaca attacgtctt cgataccgcc
      181 gccgggcgct acatgcttct gctcttcttc ggccgggcgg cggatccggg ggccgcggag
      241 gctctggcct gcgtcgctcg gcaccgcccg ctgttcgacg atatcggggc ctgcttcttc
      301 ggagtgacct cggacccgga ggatgaggcc ggcggacgcg tcgcccagca gctccccggc
      361 atccgtttct tcctcgattc ctcgggcggg ctggccgcgc tgttcggggc cgagccggcg
      421 gggaagcccg agcgacggca ctggctgctg gtcgatccga tgcttcgggc ggtcggcgcc
      481 tttccgctgg aggcgggcga ggccgcggtc gcggccctcg ccaaggcgat tgcccatatg
      541 ccgctgcccg actgggctcc ggtgctgatg gcgcccaaca tcctcgagcc agccctttgc
      601 gaacgcctgg tcgcgcacca ccgcgagacc gggggcgagc cttcgggatt catgcgggag
      661 gtggacggca agacggtgct gatcacggac ccccaccaca agatgcggcg cgaccgcgaa
      721 atcggcgacg agagcctgtg cggggtcctt cgcgcccgca tcctcgccag gctggtgccg
      781 atggtgaagc ggaccttcca gttcgaagcg acccggatgg agcgctacat cgtcggcgcc
      841 tatccggccg gggagggcca tttccggccg caccgggaca atacgaccag gggcaccgcc
      901 caccgccggt tcgccgtgac gatcaacctc aatgcgggcg attacgaggg cggcgacctt
      961 cgcttccccg aattcggcca gcggacctat cgggcgccga ccgggggcgc ggtcgtcttc
     1021 tcctgctcgc tcctccacga agcgactccg gtgacgagcg gcacgcgcta tgccttcctt
     1081 cccttcctct acgacgacgc cgccgcccgg cagcgggagg agaacaaccc gcatctgggc
     1141 gagggaatag gtgtttataa gtcggggtag ggcacgtcgt cccagcgaag gctgggacct
     1201 cggcgggtga aaggcccggt gttcttcacc ccacggggcc ccagcctgcg ctggggcgac
     1261 gaggcgagct cagccctccg tgctctgatt atgctcgccg ccggggtcgg tgattccgtc
     1321 gagggcgacc gaggctgcct cgccctggtc ggagcggctg atgtcgccca gtgagacgat
     1381 tccgaccaga ttctcgccgt cgcggctgag caccggcatc cggcggacct gggcgtcgct
     1441 catcttgatc gccacggcct cgacctcctc gtcgtcgaag caatagatca ggtcttcggt
     1501 catcacgtcg cgcaccggcg tgtcggggcc gagcccttcg gcgactccgc gcacggcgat
     1561 gtcgcggtcg gtgaccatcc cgatcaactt gtcgccgtcg ctgaccggca tggccccggc
     1621 gtcggctcgg agcatgaact gggcggcttc ctggatcggc tggtcgggcg agacggtctc
     1681 gacgtcctga ctcatgaatt cggtgacttt catcggggct ctcctggcgg ggggtgtttc
     1741 gcgcgaagaa cccgcggggc cccgaacagg gcccgcgtcc cggcgcgtcc cggtcacgct
     1801 ctggctagtc cctggggcta gtccccgggg ctagtccttc ggccggggca cgatgtcgat
     1861 ctcgaacagc ttcgcccaat tcttgccggt gacgaagagg cggtcgccct tggcgtcgta
     1921 ggcgatgccg ttcaggacag cgtccggatc gccgctggca ttctcgacgg cgagctggga
     1981 caggtcgatc cagcccttca ccgcgccgct cttcgggtcg atgcgcgcga ttcggcggcc
     2041 catccagatg ttcgcgaaga tctcgccttt cacccattcg agctcgttga ggcgctcgac
     2101 cggctggcct ttgtaggtga cggagacccg cttcttctcc tgcatggtcg cgggatcgaa
     2161 gaagcgcaga ttgctggtgc cgtcgctgag gatgatgtgc ttgccgtcct gggtcagccc
     2221 ccagccctcg cccgaatagc ggaactggcc cttgggcctg aggtccgccc ggttccagcg
     2281 gaagcccacg ccgtcctgcc aggtcaggct gagcaggtcg gacccccaat tgaccagccc
     2341 ctcgccgaaa tattggggcg ggatcgagat cgagcggagg accttgccgt cctcgagccg
     2401 aacctcgcgc accgtcgact ggcggttgag cccggtgctc tcgtagagct tgccgtcgag
     2461 gtagacgaga ccttgggtga aggcggccgg atcgtgcgga tattcgcgga cgatgcggta
     2521 gccgtcgacc gggggctcgg ccggcgccga agccgcgcag ccgggcaggg ccagagcgaa
     2581 gagcggcaat gcctgtcgaa gcttcatgat atccaccgtc tctcttcaaa gctttcatcg
     2641 gcttcacgat aggggtcggc ggcaggatcg ctgtagaggg ggctgcccgg
//
LOCUS       DASWNU010000217         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_33973, whole genome shotgun sequence.
ACCESSION   DASWNU010000217 DASWNU010000000
VERSION     DASWNU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33973"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VGB57_04245"
     CDS             <1..849
                     /locus_tag="VGB57_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEY0130594.1"
                     /translation="AAMDALFDETERALAPDQGSAASTFLGAFTILLREGLEALLIVV
                     AMLAFLRKADRSEMVRPVHWGWITALVAGLATWWAATHLISVSGANRELTEGFGSILA
                     AVILLFVGIWMHGKAQADEWQRYIREKVGQALSRKSAWFLFLLAFVAVYREVFETILF
                     FTALSAEGRLAPLIGGAATAAALLVAIAVLMFRFSRKLPIAKFFSYSSVLIAVLAVVL
                     AGKGVAALQEAGMIGVAPLDWAPRVEILGLYPTIEGLLAQAATLVILLIGFARNRRGT
                     RKLATA"
     gene            993..1493
                     /gene="ssb"
                     /locus_tag="VGB57_04250"
     CDS             993..1493
                     /gene="ssb"
                     /locus_tag="VGB57_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY0130595.1"
                     /translation="MAGVNKVILVGNLGDDPESRSLNNGGEVVNLRVATSENWKDKEG
                     NRQERTEWHRVVIFNENLGRVAKSYLRKGSKVYLEGQIQTRKWTDQSGADRYSTEVVL
                     QRFRGELVLLDSRGGGGGGGEDYGGGYDSGSSGFGGSGGGGGGSRPQSRPAPAFDSDL
                     DDDVPF"
     gene            complement(1618..2151)
                     /locus_tag="VGB57_04255"
     CDS             complement(1618..2151)
                     /locus_tag="VGB57_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY0130596.1"
                     /translation="MNEFSRLYRLDGLSAEPLQVEIEAGPDEREALARRFGLVALGAL
                     TTEAELRRTGEAATAAGTLRARVTQSCVASGDPVEAEVTEDFRIDFRPLPSDARPEEE
                     IELGEGELDVVFYEGGAIDLGEAAAQTLLLGLDPYPRSPAAEAALRDAGVKSEEEARM
                     ESSPFAALAALKGKLEE"
     gene            complement(2148..2687)
                     /locus_tag="VGB57_04260"
     CDS             complement(2148..2687)
                     /locus_tag="VGB57_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEY0130597.1"
                     /translation="MRRPPLALLSRLFGERRDRAALRPLYDAAVAAAREPAWYREGGV
                     PDTLEGRFDMLATMVALALIRLEAEGDRAKAESVTLTEIFIDDMDGTLRQIGIGDFVV
                     GKHVGKLVGALGGRLGALREAMAGGAELEPAVRRNVFRDGPASDSQVEWVAARLRALH
                     ERLSRTAMEPLLAGAFPLS"
     gene            2812..3297
                     /gene="bamE"
                     /locus_tag="VGB57_04265"
     CDS             2812..3297
                     /gene="bamE"
                     /locus_tag="VGB57_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY0130598.1"
                     /translation="MVSGRKAALLIAVAAGLATGGCTKSQDLQGYLVDETLVSAVQPG
                     VDNKESVQNTLGRPTFTGTFDQNDWYYVSRRTKNYAFNHPRVNQQTVLHVRFDPAGNV
                     ASVERKGMEQIVSIDPSGDKTPTLGRNVSIWDELFGNIGAVGAGGMTAPTGGGGPNSP
                     N"
BASE COUNT          533 a         1135 c         1142 g          559 t
ORIGIN      
        1 gcggccatgg acgccttgtt cgacgagacc gagcgcgctc tggcgccgga ccagggaagc
       61 gccgcctcaa ccttcctcgg cgccttcacg atcctgctgc gcgaggggct ggaggcgctt
      121 ctcatcgtcg tcgcgatgct cgccttcctc cgcaaggcgg atcggtccga gatggtccgc
      181 ccggtccatt gggggtggat caccgcgctc gtcgccggcc tcgccacctg gtgggccgcg
      241 acccacctga tctcggtcag cggcgccaac cgcgagctta ccgaaggctt cgggtcgatc
      301 ctggcggccg tgatcctgct cttcgtcggc atctggatgc acggcaaggc gcaggccgac
      361 gaatggcagc gctacattcg cgagaaggtg gggcaggctt tgtccaggaa gtccgcctgg
      421 ttccttttcc tgctcgcctt cgtcgccgtc tatcgcgagg tgttcgagac gatcctcttc
      481 ttcaccgcgc tgtcggccga aggacggctg gcgcccctga tcggcggggc ggcgacggcg
      541 gcggccctgc tggtggcgat cgcggtcctg atgttccggt tcagccgcaa gctgccgatc
      601 gccaaattct tctcgtacag ctcggtgctg atcgcggtgc tggcggtggt gctggccggc
      661 aagggcgttg cggcgcttca ggaggcgggg atgatcggcg tcgctccgct cgattgggcg
      721 ccgagggtgg agatactggg cctttatccg acgatcgagg ggctgctggc gcaggcggcg
      781 acgctggtca tcctgctgat cggtttcgcc cgcaaccggc gagggacgag aaagctcgcg
      841 acagcctaga accgtccttc cggcgaaagt acgtgggttc gggcttgcgc gggggtgacg
      901 gcaactgggg ataaatcctg aaatccggta gcgcgcgcca accgcctcgc ttatcgtccg
      961 cggcatattc gacacgtacg gaaggaagag cgatggcggg cgtcaacaag gtcatattgg
     1021 tcggcaatct cggtgacgat cccgagagcc gctcgctcaa caatggcggc gaagtggtca
     1081 atctgcgcgt cgccacgtcc gagaactgga aggacaagga gggcaaccgc caggagcgga
     1141 ccgaatggca tcgggtcgtc atcttcaacg agaatctcgg ccgggtcgcc aaatcctatc
     1201 ttcgcaaggg ctccaaggtt tatctcgagg gccagatcca gacccgcaaa tggaccgacc
     1261 agtcgggcgc cgaccgttat tcgaccgagg tggtcctcca gcgcttccgc ggcgagctcg
     1321 tcctcctcga cagccgcggc ggcggcggcg gcggcggcga ggattatggc ggcggctatg
     1381 acagcggcag ctcgggcttc ggcggcagcg gcggcggcgg cggcggatcg cgcccccagt
     1441 cgcgcccggc cccggccttc gacagcgacc tggacgacga cgttccgttc tgacgccaaa
     1501 ggcgtcatcc cggcgaaggc cgggatctcg gggagaccca ctgtgccttt cttgtcccga
     1561 ggtcccggct ttcgccggga tgacgggcta ttttcccacg ccgccctatt tccccgccta
     1621 ttcctccaat ttccccttca gcgccgccag cgccgcgaac gggctggatt ccatgcgcgc
     1681 ctcctcctcg ctcttcaccc cggcgtccct gagcgcggct tcggcggccg gggagcgggg
     1741 ataggggtcg aggccgagca gaagggtctg ggcggccgct tcgccgaggt cgatcgctcc
     1801 accttcgtag aagacgacgt cgagctcgcc ttcgccgagc tcgatctcct cctccggccg
     1861 ggcgtcggag ggcaggggcc ggaagtcgat tcggaaatcc tcggtcacct ccgcctccac
     1921 cggatcgccc gacgccacgc agctttgggt cactctggcc cgaagagtcc cggccgccgt
     1981 cgcggcctcg ccggttcggc gcagctccgc ctcggtcgtc aaagcgccga gggcgacgag
     2041 accgaagcgg cgggcgagcg cctcgcgctc gtccgggccg gcttcgatct cgacctggag
     2101 cggttcggct gacagcccgt cgagtcggta gaggcggctg aattcgttca cgagagcgga
     2161 aacgccccgg ccagcaacgg ttccatcgcg gttctggaaa gccgctcatg gagggcgcgg
     2221 agccgcgccg cgacccattc gacctgcgag tccgaggcgg gcccgtcgcg gaagacgttg
     2281 cgcctcactg ccggctcgag ctcggccccg ccggccatcg cctcgcgcag cgcaccgagt
     2341 cgaccgccga gcgcgcccac caatttgccg acatgcttgc cgacgacgaa gtcgccgatg
     2401 ccgatctggc gcagggttcc gtccatgtcg tcgatgaaaa tctcggtgag agtcaccgac
     2461 tccgctttgg cgcgatcgcc ttccgcctcg agccggatga gggccaaagc gaccatcgtc
     2521 gcgagcatgt cgaaacggcc ctccagagtg tccgggactc cgccctcgcg ataccaggcc
     2581 ggctcccgcg ccgcggcgac cgcggcatcg tagagcggcc tcagggcggc gcggtcgcgc
     2641 cgctcgccga acaggcggga aagcagggcc agtggaggtc tcctcatcga tgggcgcgta
     2701 cttgcgccgt tcagcttttg tcggatattg gagggcctgg ccctgccggc aaggcaggcg
     2761 ccggcgcgcg cgactcgcgc cggccggttt tacggagttt tttccaatat gatggtttcg
     2821 ggacgaaaag cggctttgct gatcgccgtc gcggcggggc tggcgaccgg cggctgcacc
     2881 aagagtcagg acctccaggg ctatctcgtc gacgagactc tggtgagcgc ggtccagccc
     2941 ggtgtcgaca acaaggaatc ggtgcagaac acgctcggcc ggccgacctt caccgggacg
     3001 ttcgaccaga acgactggta ttacgtctcc cgccggacca agaattacgc cttcaaccac
     3061 ccgcgggtga accagcagac ggtcctccac gtccgcttcg atcccgccgg caacgtcgcc
     3121 tcggtcgagc gcaagggaat ggagcagatc gtctcgatcg acccgtcggg cgacaagacc
     3181 ccgacgctcg gccgcaacgt cagcatctgg gacgaattgt tcggcaatat cggcgccgtc
     3241 ggagccggag gcatgacggc tccgacgggc gggggcgggc ccaactcgcc caactgacgt
     3301 catcccggcg cgtcatcccg gcgaacgccg ggacccatga acacggatag cggaaatacg
     3361 gaacctacg
//
LOCUS       DASWNU010000218         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_59304, whole genome shotgun sequence.
ACCESSION   DASWNU010000218 DASWNU010000000
VERSION     DASWNU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59304"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VGB57_04270"
     CDS             complement(<1..1057)
                     /locus_tag="VGB57_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0130599.1"
                     /translation="MNFESRKWRSGAVESEQVVVVDDATVARRRRRNIILAVAALVVL
                     AIALFLLFGRGGEEKQGAAAGAAKAGGAEAPAVSVIVPGRQQVARTISATGTLAARRD
                     MPVGVAGEGGMIARVLVEPGQWVGAGQTLAVIERSVQSQEAQQLAASIEVARADARLA
                     QQELERAQTLVSRGFVSKADVERRIATRDAANARVRVAQAQLGATRARIGRLDVRAPA
                     AGLVLDRNVEPGQIVSSGSGALFRIAQGGQMELLARLPQTDLSTLSVGVPVTVTPVGS
                     QQSYQGSVWQISPIIDPQTRQGEARISIPFNRELRPGGFASAAITAGATVAPLLPESA
                     VLTDDAGTYVLIVTANNT"
     gene            1254..1520
                     /locus_tag="VGB57_04275"
     CDS             1254..1520
                     /locus_tag="VGB57_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0130600.1"
                     /translation="MNLLADYGFFGWILIGALAGGLAKLIMPGRDPGGCIVTILLGVG
                     GALLAGFISHQLGWDRGNTGAGFIAAVIGAIVILFIYRLINRRR"
BASE COUNT          235 a          565 c          581 g          272 t
ORIGIN      
        1 ccgtgttgtt cgcggtcacg atcagcacgt aggtgccggc atcgtccgtc aggaccgccg
       61 attccggaag cagcggcgcg accgtcgccc cggccgtgat cgcggcggag gcgaagccgc
      121 cgggcctgag ctcgcggttg aaggggatcg agatccgcgc ttcgccctgg cgggtctgcg
      181 ggtcgatgat gggcgagatc tgccagaccg agccctggta gctttgctgc gagccgaccg
      241 gagtcacggt gacgggcacg cccaccgaga gggtggagag gtccgtctgc ggcagccggg
      301 cgagcagctc catctgccct ccctgggcga tccggaagag cgctccggag ccgctgctca
      361 cgatctggcc gggctcgacg ttgcgatcga gaacgaggcc ggcggcgggg gcgcgaacgt
      421 ccagccgtcc gatccgggcg cgcgtggcgc cgagctgcgc ctgggcgacc cggactcgcg
      481 cgttggccgc gtcgcgggtg gcgatgcgtc gctcgacgtc ggccttggag acgaagccgc
      541 gcgaaaccag cgtctgggcg cgctcgagct cctgctgagc gagccgggcg tcggcgcggg
      601 ccacttcgat gctggccgcc agttgctgcg cttcctgcga ctggaccgac cgttcgatga
      661 cggcgagcgt ctggccggca ccgacccact ggccgggctc gaccaggacc ctggcgatca
      721 ttccgccctc gcccgcaacg cccaccggca tgtcgcggcg ggccgcgagc gtgcccgtcg
      781 ccgaaatggt gcgggcgacc tgctggcggc ctggaacgat gacgctgacg gcgggcgcct
      841 cggcgccccc ggccttggcc gcgcctgccg ccgctccctg tttctcttcg cccccgcggc
      901 cgaacagaag gaacaaggct atggcgagga ccaccagggc ggcgacggcg aggatgatgt
      961 tgcggcggcg gcggcgggcg accgttgcgt cgtcgacgac gaccacttgc tcgctctcga
     1021 cggcaccgct gcgccatttg cgcgactcga agttcatagc tctcatcttt ccccacaccg
     1081 gaccgggcgg atcgccgctt cggctactgt attagccaaa tatagcacct gcctcaagtc
     1141 taaactgtcg cgcgccccca cgcggcggaa ccggggtcta cattttttgt tggggtttga
     1201 ccactcgaaa gcggtacatc cgggaacgag caagcgaggg taaggaggga ctcatgaacc
     1261 tgctggcgga ctatggcttc ttcgggtgga tcctgatcgg agcgctggcg ggcggccttg
     1321 ccaagctcat catgccgggc cgggacccgg gcggctgcat cgtcaccatc ctgctcggag
     1381 tcggcggggc cttgctcgcc ggtttcatca gccatcagct cggctgggac cggggcaata
     1441 cgggcgccgg cttcatcgcc gccgtcatcg gcgcgatcgt catcctgttc atctaccgcc
     1501 tgatcaaccg gcggcgctga tttttgcctc cccatcgcca gatggggagg gggaccaggc
     1561 gaagcctggt ggaggggtat ctggcgcagg ccggttccag ttacccctcc accatgcttc
     1621 gcatggtccc cctccccacc gcttcgcggc agg
//
LOCUS       DASWNU010000219         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_501407, whole genome shotgun sequence.
ACCESSION   DASWNU010000219 DASWNU010000000
VERSION     DASWNU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501407"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VGB57_04280"
     CDS             <1..166
                     /locus_tag="VGB57_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEY0130601.1"
                     /translation="GHTGFTGTGLWIDFDLGLAWTLLTNRVHPTRHRETGIAELRRAT
                     GEAVIAAWRP"
     gene            complement(407..1879)
                     /gene="dacB"
                     /locus_tag="VGB57_04285"
     CDS             complement(407..1879)
                     /gene="dacB"
                     /locus_tag="VGB57_04285"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY0130602.1"
                     /translation="MRGRISKIRALSLGLVLAASPLSAEEGAAGLQQRVETRLKEAGP
                     GTRFGLVVATEDGREIVAIAPEARFIPASNTKMFTTAAAYANLPAVDAPDVAGGAAVR
                     VETGRGGAPDVVLEGRGDARLSSRPDCVRNCLAALADAVAARVRAVGDVIGDDSLFPD
                     QRWSPGMSWNNIPTRSGTATSALSLDDNEVHMRVFPAEMGKPPRLELLPYYEIDNRAV
                     TVAAGPTELEFDRMPGERLVRLTGTIAVGAEPELVRLGIDDPAHHAAWRFKSLLQDRG
                     VRVTGEAAVRHRPLRPADDPAVRKGAPAARPPRSEPLASLTPPPLAADIDLTNKVSQN
                     LHAELLLRRVGLQSGSGSIADGLAAVRAMLERAGVPRPSYHFSDGSGMSTYNRVAPRG
                     MIAFLRWAAGQPWGAAWRASLPVGGVDGTLARRFKGTALENRVFAKTGTLNASSALSG
                     YMTARSGRTLLFSAYANDIPEGAAATAAMDAALLLIAEAN"
     gene            2017..>3219
                     /locus_tag="VGB57_04290"
     CDS             2017..>3219
                     /locus_tag="VGB57_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0130603.1"
                     /translation="MILLRRFLLGLWAAGLMLGPASAFAPAQRDPLAAALALPVASEL
                     VGARDAARFAWVESEAGVRNLWFAERGRPARRLTAFVQDDGQQLYDLALSVDGAQLAF
                     VRGGDPESRDDALPNAAWDSEPPRQQIFLAPTAGGEPLRIGDGHSPTFSPDGTGVAFT
                     RRGEIWLWRTGGEARKLASAGGEIRRLQWSPDGARLLFVSHRGDYSFIALLDPGQPRI
                     RFLDPGLGFSVEPVFSPDGGRVAYIRHLDAPAGAAPGSGPYWSIRVVDTAGGAARELW
                     AAPPGAGGRYYESRGQDLFWSRDGQLVFPWERSGWLHPYALDAARGGTPRELTPGPFE
                     VEMFMLGADGRSLVWAGNSGDLNRRHIWRRPLSGGAPERVTGGTGIETAPVLGGTALA
                     VIATDVSRP"
BASE COUNT          439 a         1183 c         1105 g          492 t
ORIGIN      
        1 cggccacacc ggcttcaccg gcaccggcct gtggatcgac ttcgacctcg gccttgcctg
       61 gaccctgctc accaaccggg tccacccgac ccggcatcgg gagaccggca tcgccgagct
      121 tcgccgggcg acgggcgagg cggtgatcgc ggcctggcgc ccctaaattc ctcccccaga
      181 tttctcgggg agggggaccg cgcgaagcgc ggtggagggg ttcttcgcgc gctgaagagc
      241 ccctccacca tgcttcgcat ggtccccctc ccctgccaat gcaggggagg aattatccga
      301 tctcacaatg tcaaagagcc ccccgagccc ctcacgactc ggcatgaaga acaatatagg
      361 aacggcaggc cccgatcccc tacagatcga tccgtttcgg aagttgctaa ttcgcctcgg
      421 cgatcaacag cagggcggcg tccatcgccg ccgtcgccgc ggcgccttcg gggatgtcgt
      481 tggcataggc cgagaacagc aaggtccggc cgctcctagc cgtcatgtag cccgacagcg
      541 ccgagcttgc gttcagggtc ccggtcttgg cgaagacgcg attctccagt gccgtgccct
      601 tgaaccgcct cgccagggta ccgtcgactc cgccgaccgg cagcgaggcg cgccacgccg
      661 ctccccaggg ctggcccgcc gcccagcgca ggaaggcgat catccctcgc ggcgcgaccc
      721 ggttgtaggt cgacattccc gagccgtcgg agaaatggta gctcggccgc ggcaccccgg
      781 cgcgctccag catggcgcgg acggccgcca gcccgtcggc tatcgagccg cttcccgact
      841 gaagcccgac ccggcgcagg agcagctcgg cgtggaggtt ctggctgacc ttgttggtca
      901 ggtcgatgtc ggccgcgagc ggcggcggag tgaggcttgc gagcggctcg gaccggggtg
      961 ggcgagcggc aggcgctccc ttgcgcaccg ccgggtcgtc ggcgggtcga agcgggcggt
     1021 ggcgaaccgc cgcctcgccc gtcacccgca ccccccggtc ctggagcagc gacttgaagc
     1081 gccaggcggc atgatgcgcc ggatcgtcga tcccgagccg aacaagctcg ggctcggccc
     1141 cgaccgcgat cgttccggtc agccggacca gccgctctcc cggcattcgg tcgaactcca
     1201 gctccgtcgg accggccgcg accgtcaccg cccgattgtc gatttcgtaa taaggcagga
     1261 gctcgagcct cggcggcttg cccatttccg cggggaagac ccgcatatgg acctcgttgt
     1321 cgtcgaggct gagcgccgag gtggcggtcc cggagcgggt cgggatgttg ttccagctca
     1381 tcccggggct ccagcgctga tccgggaaga ggctgtcgtc gccaatcacg tcgccgaccg
     1441 cccggaccct ggccgcgacc gcgtccgcga gtgccgccag gcagttccgg acgcagtcgg
     1501 ggcggctcga aaggcgggcg tcgccccggc cttcgaggac gacgtcgggc gcgccgcccc
     1561 tccccgtctc caccctcacg gcggcaccgc ccgcgacatc cggcgcgtcg accgctggaa
     1621 gattggcgta ggcggcggcg gtcgtgaaca tcttggtgtt ggacgccggg atgaagcgcg
     1681 cttccggagc gatggcgacg atctcccggc cgtcctccgt cgcgacgacg aggccgaagc
     1741 gggtgcccgg ccccgcttcc ttcagccggg tttcgacccg ctgctggagc ccggcggctc
     1801 cctcctcggc cgaaagcggc gaggcggcca gaacgaggcc tagggaaagc gccctgatct
     1861 tcgaaatcct gcccctcatc gccacgaccc tagagccaaa gccgggcggg gcaatgcccc
     1921 aggactcatt cagagggttc cagcggccat aaccctcgcg catggcgggg gccgcggcgc
     1981 ttgtcggagg cggatggcgg cttcacaagg ggtcggatga tcttgttgag acggtttcta
     2041 cttgggctct gggcggccgg gctcatgctc gggccggcct cggccttcgc tcccgcgcag
     2101 cgcgacccgc ttgccgccgc tctcgccttg ccggtcgcga gcgagctcgt cggcgctcgc
     2161 gatgccgctc gtttcgcctg ggtggaaagc gaagcgggag tccgcaattt gtggttcgcc
     2221 gagcgcggcc ggccggcgcg caggctcacc gccttcgttc aggacgacgg gcagcagctt
     2281 tacgatctcg ccctgagcgt agacggcgcc cagctcgcct tcgttcgagg cggcgacccg
     2341 gaatcgcggg acgatgcgct tcccaacgcc gcctgggact ccgagccgcc ccgccagcag
     2401 atcttccttg ccccgacggc gggcggcgag ccgcttcgga tcggcgacgg ccattcgccc
     2461 accttctcgc cggacggcac gggggtcgct ttcacccggc gcggcgagat ctggctttgg
     2521 cggaccggcg gcgaggcccg caagctggcc tcggccggag gcgagatcag gcggctgcaa
     2581 tggtcgcccg acggagcccg actcctcttc gtcagccatc gcggcgatta cagcttcatc
     2641 gcccttctcg accccggcca gccccggatc cgcttcctcg accccggcct cggcttctcg
     2701 gtcgagccgg tcttctcgcc cgacggcggt cgggtcgcct atatccgcca tctcgacgcc
     2761 ccggccggag ccgcgcccgg cagcggtccc tattggtcga tccgggtcgt cgacaccgcc
     2821 ggcggagcgg cgcgcgagct ttgggccgct ccgccgggcg ccggcgggcg ctattacgaa
     2881 tcgcggggcc aggacctgtt ctggagccgg gacggccagc tcgtcttccc ctgggagcgc
     2941 agcggctggc tccaccccta tgcgctcgac gccgccaggg gcggcacccc cagggagctg
     3001 actcccggcc ccttcgaggt cgagatgttc atgctcggcg ccgacggccg ctcgctcgtc
     3061 tgggccggca attcgggcga tctcaaccgg cggcacatct ggcggcggcc cctttcgggc
     3121 ggagcgcccg agcgggtcac gggcgggacc gggatcgaga ccgctccggt gctcggcggc
     3181 accgctctcg ccgtgatcgc gaccgacgtc tcgcgcccc
//
LOCUS       DASWNU010000220         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_86009, whole genome shotgun sequence.
ACCESSION   DASWNU010000220 DASWNU010000000
VERSION     DASWNU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86009"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VGB57_04295"
     CDS             complement(<1..370)
                     /locus_tag="VGB57_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130604.1"
                     /translation="MRDSAPRGEGERQEAARRRTRWLIFVALFLAGILPGFYLGSQGG
                     VALIVSRDAIWPPALVAAFLGLYLVGVVGGGIVLGKLADELDRQRAYKAVSFAGTLMM
                     IAYPVWYVLWRGGFVPEPIHW"
     gene            complement(360..575)
                     /locus_tag="VGB57_04300"
     CDS             complement(360..575)
                     /locus_tag="VGB57_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130605.1"
                     /translation="MKNRLKVLRAERDWSQAELGGRLGVSRQAVNAIETGKYDPSLPL
                     AFKLSRLFDVPIEEIFDDGFGGSCDAR"
     gene            complement(824..2320)
                     /locus_tag="VGB57_04305"
     CDS             complement(824..2320)
                     /locus_tag="VGB57_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEY0130606.1"
                     /translation="MKKILLAASLLAFAAPAFSQDPPPATEASAPVPAPIVSVTRHTG
                     TFGGQRVRYTATASETYLKAADGTPKASLFSIAYVKQPRDPNRPVAFLFNGGPGSGSL
                     WLHMGAFGPKRVAIPSDARDDGAPPFPILDNPDSLLDVTDIVFIDPVGTGFSRPLGKT
                     EGKEYWGVTQDAKSVAEFIRIWLGENGRWNSPKFLGGESYGTTRSAAVVNQLEGAFND
                     VSLNGIILISTILDFGAQANVPGTEMPYVLFLPSMAAAAFHHGKAPADAGSLEGFVQE
                     ARDFAAGDYATALFKGQKLQGEERAAIRRRLARFTGLSERFLEAADLRVSDDRFYKEL
                     LRDRGLVIGRLDARYTGRDHDNAGERPDNDPSFYGIDAGYTAALNAWVRDGLGFRTDR
                     QYATIAGLGNWEWDLADQGGEARYYKNVAPYLGRAMRENSGLRLFVGQGYFDFATPFF
                     GAEYSLSRTGLPQDRIVFRYYGSGHMMYVRDEDRTKLSRDIREFIRGR"
     gene            2444..2890
                     /gene="queF"
                     /locus_tag="VGB57_04310"
     CDS             2444..2890
                     /gene="queF"
                     /locus_tag="VGB57_04310"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY0130607.1"
                     /translation="MDLTHLGRSTPMPASPGEAKLDYVSNPRPGSPYLVRFTAPEFTS
                     LCPVTGQPDFAHLVIDYAPGGTIVESKSLKLFLSAFRNHCGFHEDVTVGIAERLAAEM
                     KPLWLRIGGYWYPRGGMPIDVFWQSGEPPRGLWVPAQDVPGYRGRG"
     gene            2892..3926
                     /locus_tag="VGB57_04315"
     CDS             2892..3926
                     /locus_tag="VGB57_04315"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF006083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0130608.1"
                     /translation="MKVAIFGAGSVGCFIGGAWAAAGVPVSFVGRERIGREIETNGLT
                     LTDYSGWRTRLAPEQVDFSTRPAGLAQADVIALCVKSNATAAAAKEIARNAKKGALVV
                     SFQNGVSNVDLLRSVLKTRFEAVAGMVPYNVAALGHGRFHKGVAGDLIAEDVPALREL
                     AGRIGGGPARLDLSSDMPAIAWGKLLINLNNAVNALSGRTLLEQLKERDYRRVVAASV
                     MEALGLLEAAGIEPAKIGPVPPRLLPHVIASPDLIFNNLFLRIQKIDARARSSMADDL
                     KAGRPTEIAYLNGEVVTLAKKLGRRAPVYEAIVSLICQAEAGVELLWEPARLRAHVLE
                     GHKSAPIFGY"
     gene            3987..>5581
                     /locus_tag="VGB57_04320"
     CDS             3987..>5581
                     /locus_tag="VGB57_04320"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0130609.1"
                     /translation="MRLFATVSLLALALAGCARSTDRTPPASPAAAAAAQGSRQIPTQ
                     LPTDVRPIQYTINATPDAANLRFTARTDIDIEVLRATDSITLNAADLEFGNVSLGGGS
                     EDAPLALNPRDIDVSEEKQTATFRFGREIAPGRYRLTINYKGKIYTQAAGLFALDYEG
                     ATGKERALFTQFEAPDARRFFPGWDEPQFRTPYNLNVTIPADQDVVGNMPQAGVRTNA
                     NGTKTITFQTTPAMSSYLLFLAVGKFDRITTTSAGTEIGVVTKRGDGEKGRWALEGSA
                     QIVPWYNEYFGTPYPLPKLDNVAGPGSSQFFGAMENWGAIFSFESILLVDPAITTEAT
                     KQRIFEVAAHEIAHQWFGNLVTMSWWDDLWLNEGFASWMATKATTALHPEWDPELGTV
                     SGREAAITLDSVASTHPVVQRISTVEQISQAFDAITYQKGEAVITMLEDYVGEDAWRR
                     GVQAYIRTHRLGNTQTDDLWQAVERAAGKPVTAIAHDFTLQPGVPLIRVENAACRGGK
                     TEVSLRQSEFTRDRPDVTAKAWRV"
BASE COUNT          954 a         1946 c         1806 g          875 t
ORIGIN      
        1 cccaatggat cggctcgggg acgaaaccgc ccctccagag cacgtaccag accggatagg
       61 cgatcatcat caaggtgccg gcgaagctca ccgccttgta ggcgcgctgg cggtcgagct
      121 cgtccgccag cttgcccagg acgatgccgc ccccgaccac accgacgaga tagagcccca
      181 ggaacgccgc cacgagggcc ggcggccaga tcgcgtcgcg gcttacgatc agagcgacac
      241 ctccctgaga gcccaggtag aagcccggga ggatccccgc caggaacaga gcgacgaaga
      301 tcagccacct tgtgcgacgc cgagcggcct cctgccgctc cccctccccg cgcggcgcgc
      361 tatcgcgcat cgcacgagcc cccgaaaccg tcatcgaata tctcctcgat cgggacgtcg
      421 aacagccggc tgagcttgaa ggcgagtgga agcgaaggat cgtatttgcc ggtctcgatg
      481 gcgttgaccg cctggcgtga gactccgagc cggccgccaa gctccgcctg gctccagtcg
      541 cgctccgccc tcaggacctt caaccgattc ttcacatacc cctcaatgtc caccgcactg
      601 gcctcgtccc gccggcagcc catccgccgg ccatggcgac tcgatagccc cagcctgaca
      661 taatgtcaag catagctgac ttattgtcag gcacggaccg cagaaagcca tttctgctgg
      721 cctttgcata ttcgtcgccc cagcgaaggc tggggccccg tcgggtgaag aacgccgtgt
      781 ttttacccct tgggatccca gccttcgctg ggatgacgag gtttcacctc ccccggatga
      841 actcccttat gtcgcgcgaa agcttcgtcc ggtcctcgtc ccgaacgtac atcatgtggc
      901 ccgagccata ataacgaaag acgatccggt cctgcggaag gccggtgcgc gacaaagaat
      961 attcggcgcc gaagaagggc gtggcgaagt cgaaatagcc ctggccgacg aacaggcgga
     1021 ggcccgaatt ctcccgcatc gcccggccca gatagggcgc gacattcttg tagtagcgcg
     1081 cctcgccgcc ctggtcggcc agatcccact cccagttgcc gagcccggcg atcgttgcat
     1141 attggcggtc ggtcctgaac cccaggccat cgcggaccca ggcgttcagc gccgcggtat
     1201 agccggcgtc gatgccgtag aagctcgggt cgttgtccgg cctttccccc gcgttgtcgt
     1261 gatcgcggcc ggtatagcgg gcgtcgaggc ggccgatgac gaggcccctg tcgcggagca
     1321 gctccttgta gaagcgatcg tcgctgaccc gaaggtcggc ggcctccagg aagcgctcgg
     1381 aaaggccggt gaagcgggca agccggcggc ggatggcggc gcgctcctcg ccctgcagct
     1441 tctggccctt gaacaaggcg gtcgcatagt cgccggcggc gaagtcgcgc gcctcctgga
     1501 cgaagccttc caggctgccg gcgtcggcgg gcgccttgcc gtgatggaaa gcggccgcgg
     1561 ccatcgacgg gaggaatagc acatacggca tctcggtgcc cggcacgttg gcctgggcgc
     1621 cgaagtcgag gatggtcgag atgaggatga tcccgttcag cgagacgtcg ttgaaggcgc
     1681 cctcgagctg gttgacgacg gcggccgagc gggtggtccc gtagctctcg ccgccgagga
     1741 acttgggcga gttccagcgg ccgttctcgc ccagccagat gcggatgaac tccgcgaccg
     1801 acttcgcatc ctgggtcact ccccaatatt ccttgccctc ggtcttgccg agcggccggc
     1861 tgaagccggt gccgaccgga tcgatgaaga cgatgtcggt gacgtcgagc aggctgtccg
     1921 gattgtcgag gatcgggaaa ggcggcgcac cgtcgtcgcg agcatcagaa gggatcgcca
     1981 ccctcttcgg cccgaaggcc cccatgtgca gccagaggga cccggagccg gggccgccgt
     2041 tgaacaggaa ggcgaccggc cggttcggat cgcgcggctg cttcacgtag gcgatcgaga
     2101 agaggctcgc cttgggcgtt ccgtcggcgg ccttgagata ggtctccgaa gccgtcgccg
     2161 tgtacctcac gcgctgcccg ccgaaggtgc cggtgtgacg ggtgaccgag acgatcggcg
     2221 cgggcaccgg cgcactggcc tccgtcgccg gcggagggtc ctggctgaag gcgggcgcgg
     2281 cgaacgccag gagcgaggcg gcgagcagga tcttcttcat ggcgcgagct atcgcactcg
     2341 gccagccgga gtcaaagtgg acggcccacg gcgggggagc cttggtttgc cccctcgccg
     2401 cgaccgcctt tcccttcccg gccgccttca ctagagagcg gccatggacc tcacccatct
     2461 cggccgttcc actccgatgc ccgcatcgcc cggggaggcg aagctcgact atgtctccaa
     2521 tccgcggccc ggcagcccct atctcgtgcg cttcaccgcg ccggaattca cctcgctctg
     2581 cccggtcacc ggccagcccg atttcgccca cctcgtcatc gactatgcgc ctggcgggac
     2641 catcgtcgaa tccaagtcgc tgaagctgtt cctctcggcc ttccgcaacc attgcggatt
     2701 ccatgaggat gtgacggtgg gcatcgccga gcggctggcc gcggagatga agccgctctg
     2761 gcttcggatc ggcggctatt ggtatccgcg cggcgggatg ccgatcgacg tcttctggca
     2821 atcgggcgag cctccccggg gcctgtgggt cccggcccag gacgtgcccg gctatcgcgg
     2881 gcgggggtga gttgaaggtc gcgatcttcg gggcggggtc ggtcggctgc tttatcggag
     2941 gcgcctgggc ggcggcgggc gtaccggtca gcttcgtcgg gcgggagcgg atcggccggg
     3001 agatagagac gaacggcctc acgctcaccg actattcggg ctggcggaca aggctggcgc
     3061 ccgagcaggt cgatttctcg accaggcccg ccgggctcgc ccaggccgac gtcatcgcct
     3121 tgtgcgtcaa gagcaatgcc acggcggcgg ccgcgaagga gatcgcccgc aatgcgaaga
     3181 agggcgctct ggtggtcagc ttccagaacg gcgtgagcaa cgtcgacctg ctccgttcgg
     3241 tgctgaagac gcggttcgag gccgtcgcgg gaatggtgcc ctacaatgtc gccgcgctcg
     3301 gccacggccg ctttcacaaa ggcgtggccg gagacctcat cgccgaggac gtgccggccc
     3361 tgcgcgagct cgccgggcgg atcggcggcg gtccggcgcg actggacctg tcgtcggaca
     3421 tgcccgccat cgcctggggc aaattgctca tcaacctcaa caatgcggtc aacgcgctgt
     3481 cgggccggac cctgctcgag cagctgaagg agcgcgacta tcgccgggtc gtcgccgctt
     3541 ccgtcatgga agcgctcggc ctgctcgaag ccgccgggat cgagcctgcg aagatcggcc
     3601 cggtaccgcc caggctgctc ccgcacgtca tcgcctcgcc ggacctcatc ttcaacaacc
     3661 tcttcctcag gatccagaag atcgatgcga gggcgcgctc ctcgatggcc gacgatctca
     3721 aggcggggcg cccaaccgaa attgcttatt tgaacgggga ggtcgtgacc ctggcgaaga
     3781 agctggggcg ccgggcgccg gtctacgagg cgatcgtctc actgatctgc caggccgagg
     3841 cgggagtcga gctgctctgg gagccggcca ggcttcgagc ccatgttctc gaaggccaca
     3901 agtcggcgcc gatcttcggt tattgaggtt tgacaagggc ttggcgcccc cgcaggagct
     3961 caggcacgtt cccaccggag ttctccatgc gcctgttcgc caccgtttcc ctccttgccc
     4021 tcgccctcgc cggctgcgcc cgcagcaccg accggacccc cccggcctcc cccgccgccg
     4081 ccgcggcggc gcaaggctcg cgccagatcc cgacccagct gccgaccgac gtccgcccga
     4141 tacaatatac gatcaacgcc acgcccgacg ccgcgaacct gcgcttcacc gcccgcaccg
     4201 acatcgacat cgaggtcctt cgggcgacgg acagcataac cttgaacgcg gccgacctcg
     4261 aattcggcaa cgtctcactg ggcggcggaa gcgaggacgc gcccctcgcc ctcaacccgc
     4321 gcgacatcga cgtcagcgaa gagaagcaga ccgcgacctt ccgcttcggg cgcgagatcg
     4381 cgcccggccg ctatcggctg acgatcaact acaagggcaa gatctacacc caggcggccg
     4441 gcctgttcgc gctcgactac gaaggcgcta cgggcaagga gcgcgccctc ttcacccagt
     4501 tcgaggcgcc cgacgcccgc cgcttctttc ccggctggga cgagccccag ttccgaaccc
     4561 cctacaatct caacgtcaca attcccgccg atcaggacgt ggtcggcaac atgccgcagg
     4621 cgggggtccg gaccaacgcc aacggaacga agacgatcac cttccagacg actccggcca
     4681 tgtcgagcta cctgctcttc ctcgcggtcg gcaagttcga ccggatcacc accacctcgg
     4741 ccgggaccga gatcggggtc gtcaccaagc gcggcgacgg cgagaagggc cgctgggcgc
     4801 tcgaaggctc ggcccagatc gtcccctggt acaacgaata tttcggaacg ccctacccgc
     4861 ttcccaagct cgacaacgtc gccggcccgg gcagcagcca gttcttcgga gcgatggaga
     4921 attggggcgc gatcttctcg ttcgagtcga tcctcctcgt cgatcccgcc atcaccaccg
     4981 aagcgaccaa gcagcggatc ttcgaggtcg ccgcccacga gatcgcccac cagtggttcg
     5041 gcaacctcgt caccatgagc tggtgggacg acctctggct gaacgagggc ttcgcctcct
     5101 ggatggcgac caaggcgacc accgccttgc atcccgaatg ggatcccgag ctcggcacgg
     5161 tgtcgggccg cgaggcggcc atcacgctcg acagcgtcgc cagcacccac cccgtcgtcc
     5221 agcgcatcag caccgtcgag cagatcagcc aggccttcga cgcgatcacc taccagaagg
     5281 gcgaggcggt gatcaccatg ctcgaggatt atgtcggcga ggatgcatgg cggcggggcg
     5341 tgcaggccta tatccgcacc catcggctcg gcaacaccca gacggacgac ctctggcagg
     5401 cggtcgagcg ggcggcgggc aagccggtca ccgccatcgc ccacgatttc acccttcagc
     5461 cgggcgtgcc cctgatccgg gtagagaacg ccgcctgccg cggcggcaag accgaagtct
     5521 cgcttcgcca aagcgagttc acccgcgacc ggccggacgt gacggccaag gcctggcggg
     5581 t
//
LOCUS       DASWNU010000221         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_163040, whole genome shotgun sequence.
ACCESSION   DASWNU010000221 DASWNU010000000
VERSION     DASWNU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163040"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VGB57_04325"
     CDS             complement(<1..355)
                     /locus_tag="VGB57_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0130610.1"
                     /translation="MSTRSGAEAASADASLADVAYTHKGLTYPLGRRGPELGELIQIA
                     DGVGWARLPVPGSLKHINIWALDDGDGVALVDTGLDIADCRQAWEALFAGPLAGRAVT
                     RILVTHFHPDHVGLAG"
     gene            complement(333..1034)
                     /locus_tag="VGB57_04330"
     CDS             complement(333..1034)
                     /locus_tag="VGB57_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEY0130611.1"
                     /translation="MAQPLMPHATAAWLVDNTALTFQQIAEFCGLHILEVQAIADDTA
                     ATKLTGRDPLRAGELTVEEIERGPKDPNYRLQIQKEPDKATRTRGPRYTPVSKRQDKP
                     DGINWIIRNHPEVTDGQISKLIGTTRTTISAIRDRSHWNIANIQPKDPVTLGLCSQRE
                     LDALVAKAAKAAGVEPSANDGRIEQDREALIEQLRAERDAAAREAEAAHAAEASGVAV
                     GGASQDLGFVDPFRR"
     gene            complement(1146..1769)
                     /locus_tag="VGB57_04335"
     CDS             complement(1146..1769)
                     /locus_tag="VGB57_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0130612.1"
                     /translation="MAEKLVLHEYAASGNCYKVRLTAARLSLPLERREYDILKGETRT
                     PHFLGTINANGRIPVLQADERFIPESNAACFYLADGTPLIPEKRFDRADMLRWMFFEQ
                     YSHEPNVATLRFWLAFIGEANLTEAQRDQIEPRRAAGNAALKVMNDHLSAHNFFTAGR
                     LTLADIALYAYTHVAEEGGFDLSAYPAVRAWLDRVASAPGHVTMDAA"
     gene            complement(1833..1949)
                     /locus_tag="VGB57_04340"
     CDS             complement(1833..1949)
                     /locus_tag="VGB57_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HEY0130613.1"
                     /translation="MAGVPEPETWAMLIVGYGFVGGAIRRHRSRSEATPSLA"
BASE COUNT          325 a          603 c          727 g          334 t
ORIGIN      
        1 agccggcgag gccgacatgg tccggatgga aatgggtgac gaggatgcgg gtgaccgcgc
       61 ggccggcgag cgggccggcg aacagggcct cccacgcctg gcggcagtcg gcgatgtcga
      121 ggccggtatc gaccagagcg acgccgtcgc cgtcgtcaag cgcccagatg tttatatgct
      181 tgagcgagcc cgggaccggc agccgcgccc agccgacgcc gtcggcgatc tggatcagct
      241 cgcccagctc gggcccgcgc cggccgagcg ggtaggtgag gcccttgtgg gtgtaagcga
      301 cgtcggcgag cgaggcgtcg gcggacgccg cctcagcgcc ggaacgggtc gacaaagccg
      361 aggtcttggc tggccccgcc gacggcgacg ccggaagctt cggcggcatg ggcggcctcg
      421 gcttcgcggg cggcggcatc gcgctcggcg cgcagctgct cgatcagcgc ctcgcggtcc
      481 tgctcgatgc gcccgtcatt ggcgctcggc tccacgcccg cggctttggc ggccttggcg
      541 acgagggcat cgagctcgcg ctgcgagcag aggccgagcg tcaccgggtc cttcggctgg
      601 atgttggcga tgttccagtg gctgcggtcg cggatcgccg agatggtggt ccgggtcgtg
      661 ccgatcagct ttgaaatctg cccgtccgtc acttcgggat ggttgcggat gatccagttg
      721 atgccgtcgg gcttgtcctg gcgcttcgaa accggcgtat agcgcggccc tcgcgtccgg
      781 gtcgccttgt ccggctcctt ctggatctgc aggcggtaat tggggtcctt cgggccgcgc
      841 tcaatctcct ccaccgtgag ttcgccggcc cgaagcggat cgcgcccggt gagcttcgtc
      901 gcggcggtat cgtcagcgat cgcctggacc tcaaggatgt ggagcccgca gaattcggcg
      961 atctgctgga aagtaagggc ggtattgtcg accagccagg cagcggtcgc gtgaggcatg
     1021 agcggctggg ccacttgaat ctcccagaaa tgaaagtggc cgccccttac cggagcggcc
     1081 gaaacgtggc ccggatgtaa gctgccaggg ggtcggaagc aagataaagc ttacaccgga
     1141 ctgtcctacg cagcgtccat tgtaacatgt ccgggggccg aggcgacccg gtcgagccaa
     1201 gccctgaccg ccgggtaggc ggagaggtcg aagccgccct cctccgcgac atgagtgtag
     1261 gcatagaggg cgatatcggc gagcgtgaga cgtcccgccg tgaagaagtt gtgggcggaa
     1321 agatggtcgt tcatcacttt gagggcggcg ttgccggcgg cgcgccgggg ctcgatctgg
     1381 tcccgctgag cctcagtcaa attggcttcg ccgatgaagg cgagccagaa gcgcagggtc
     1441 gcgacattgg gctcgtggct gtactgctcg aagaacatcc agcgcagcat gtcggcgcgg
     1501 tcgaagcgct tttccggaat gagcggcgtg ccgtcggcca ggtagaagca ggcggcgttg
     1561 ctctcgggaa tgaagcgctc gtccgcctgg aggaccggga tgcggccgtt ggcgttgatc
     1621 gtgccgagga aatgcggcgt ccgggtctcg cccttgagga tgtcgtactc ccggcgctcg
     1681 agcggaaggc tgaggcgggc ggcagtcagg cggaccttgt agcaattccc ggaggcggca
     1741 tattcgtgaa gaacgagttt ttccgccatc gccccaacac aaaaatagga gcggcgcccg
     1801 cttcagcaga cgccgctccg atattacaaa ggtcaggcca ggctaggcgt ggcttccgac
     1861 cgcgaccggt gacgccggat ggcgccgccc acgaagccgt agccgacgat caacatcgcc
     1921 caggtctcgg gctcgggaac gccggccata gagctgtaga tgaagttctc gtggatgtag
     1981 gtggggacg
//
LOCUS       DASWNU010000222         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_293400, whole genome shotgun sequence.
ACCESSION   DASWNU010000222 DASWNU010000000
VERSION     DASWNU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293400"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VGB57_04345"
     CDS             <1..395
                     /locus_tag="VGB57_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457609.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HEY0130614.1"
                     /translation="QADCAVFRTEQTLAEGQARIDEIYRQMDDVAITDRSLIWNTDLV
                     ETLELDNLIAQAVVTMHSAANRKESRGAHMHEDYPQRDDSNWMKHTAAWFEGWGGQGG
                     QVRIDYRPVHEYTLTDEIKYIEPKARVY"
     gene            620..1072
                     /locus_tag="VGB57_04350"
     CDS             620..1072
                     /locus_tag="VGB57_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130615.1"
                     /translation="METPHVRVVADCATGTGSMLQVGSGQIESNSAIAGAACGINSNF
                     PIRNLEWLGRKATRFGALDVLRVVDSGDERIYAVDNAGVLVASEIFPRGKGSGSCLQQ
                     EPLAVETTLPAEDMFSAESLHKSFAAARFQSPPKAYVGDLWLGTRRCV"
     gene            complement(1167..>1781)
                     /locus_tag="VGB57_04355"
     CDS             complement(1167..>1781)
                     /locus_tag="VGB57_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130616.1"
                     /translation="SGFALVPRRFREEARPLARGLATIFRRGEWPEGWPGAEALEDSA
                     NPGLLLRLEASIFELERFQRLAIEDVTRIILCFHQSVREEIIERTPAHRVAPYPPGEK
                     ETADTHPIEMRTLSTLDLSEFSGGSTFEDAVRWHKAMIAHGVRLGQPVKCVKLDEGSW
                     GATLRIGLSMPMVVERAALDGEALRRSFAEDMARVRAALEAVAA"
BASE COUNT          311 a          580 c          599 g          291 t
ORIGIN      
        1 tgcaggccga ttgcgccgtc ttccgcaccg agcagacgct cgcggaaggg caggcgcgga
       61 tcgacgaaat ctaccggcag atggacgacg tcgcgatcac cgaccgcagc ctgatctgga
      121 acaccgatct cgtcgagacc ctcgagctcg acaacctcat cgcccaggcg gtggtgacga
      181 tgcactcggc cgccaaccgc aaggaaagcc ggggcgccca catgcacgag gattatccgc
      241 agcgcgacga ctcgaactgg atgaagcaca ccgcggcctg gttcgaaggc tggggcggcc
      301 agggcggcca ggtgcgcatc gattaccggc cggtccacga atatacgctg accgacgaga
      361 tcaaatatat cgagcccaag gcgcgggtct attgagagta atggcccggg cgctcgcact
      421 ctggcttctt tgtgttgcgg gcgccgcctg cgcgtcggag tcgaagccgg gaccggatcc
      481 ccgagcgagg gcggcgttcg aacggacgaa gcgcactcag gagacctacg ccctctattc
      541 gtggaattgg gtgaagggtt ccggattcca aaccggcccg cactggtccg ccgaattcca
      601 tcggggcaat tttcatcggg tggagactcc tcacgtccgg gtcgtcgccg actgcgcgac
      661 cggcacgggc tcgatgctcc aggtcggctc cggccagatc gagagcaatt cggcaatcgc
      721 cggggccgcc tgcggaatca actccaattt cccgatccga aatctggaat ggctcggccg
      781 gaaagccact aggttcggag cgctggacgt gcttcgagtg gtcgattcgg gggacgagcg
      841 tatctatgcc gtcgacaacg cgggcgtcct ggttgcgtcc gaaatattcc cgcggggcaa
      901 gggctcgggc tcctgccttc agcaggagcc gctggccgtc gagacgacct tgccggccga
      961 ggatatgttt tccgcggaat cgcttcacaa gagcttcgcg gccgccaggt ttcaatcccc
     1021 gcccaaggct tatgtgggcg atctgtggct cggcaccaga cgctgcgtgt gatcgctcca
     1081 cgcaacggtg gttgcgagat catggcggcg acattgccgg gagagggttg caggcgtcac
     1141 cccggtcgga gagatatcaa tgacggtcag gcggccaccg cctccagcgc ggcccggaca
     1201 cgcgccatgt cctcggcgaa gctccgccgc agcgcttccc cgtccagcgc ggcccgctcg
     1261 accaccatcg gcatcgacag gccgatgcga agggtcgcgc cccagctgcc ctcgtccagc
     1321 ttcacgcatt tcaccggctg gccgaggcgg accccgtggg cgatcatggc cttgtgccat
     1381 ctcaccgcgt cctcgaaggt cgagccgccc gaaaattcgc tgaggtcgag ggtcgacaaa
     1441 gtacgcatct cgatcgggtg ggtgtcggcc gtctccttct cgccgggcgg atagggggcg
     1501 acccggtggg cgggggtccg ctcgatgatc tcctcgcgaa ccgactggtg gaagcacagg
     1561 atgatccggg tcacgtcttc gatggcgagc cgctggaagc gctcgagctc gaagatcgag
     1621 gcctcgaggc ggagcagcag gccgggattg gcgctgtctt caagcgcctc cgccccgggc
     1681 cagccttccg gccattcgcc tcggcggaag atcgtcgcaa gcccccgggc gaggggccgg
     1741 gcctcttccc ggaaccggcg cgggacgagt gcgaagccgc t
//
LOCUS       DASWNU010000223         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_190708, whole genome shotgun sequence.
ACCESSION   DASWNU010000223 DASWNU010000000
VERSION     DASWNU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190708"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..970)
                     /locus_tag="VGB57_04360"
     CDS             complement(86..970)
                     /locus_tag="VGB57_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0130617.1"
                     /translation="MADWSDGYWTSSDGLRLHYRDYAGSAARPPILCIPGLTRNARDF
                     EGVADRLKGEWRLICVELRGRGQSDHAKDPMTYVPPTYWQDLEALIAALELKRFVLFG
                     TSLGGLITMLLALAGKERIAGAVLNDIGPELEQGGLEHIRSYVGRSQSWPTWLHAARF
                     LCEAQSDRYPDWDIGQWLAYAKRLCRLNRSGRVVFDYDMRIAEPFRQPGGDSGFDLWS
                     AFAALKGVPSLVVHGGISDLLSRATVERMLAENPDMEAATVPNVGHAPTLEEPEAVAG
                     IDRLLARIVAGAEASSRT"
     gene            1007..2419
                     /locus_tag="VGB57_04365"
     CDS             1007..2419
                     /locus_tag="VGB57_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY0130618.1"
                     /translation="MTQVPQAATYRADPAILDLGDAFYDPVEAADFPQTLLRFRNDRW
                     APAVGLEALGVEEWLRHFGRFEPLPDNLPRPLALRYHGHQFRVYNPDIGDGRGFLFAQ
                     LRDGDGRLLDLGTKGSGRTPYSRFGDGRLTLKGGVREVLATEMLEALGVETSKTFSLI
                     ETGEALERGDEPSPTRSAVLVRLQHSHIRIGTFQRLAYLQETGNLTKLTDYCLRHYYG
                     DEGGGDDAPARLLAHVAREAARLAASYIAAGFVHGVLNSDNIAITAQSFDYGPWRFTP
                     YWDGHFTAAYFDHAGLYAFGRQPEAIHWDVLQLAVSLRTIAEADVLNPALDAFPPEFQ
                     RRLVAAIFGRLGIRPSGQEEEDAALLVALERALAAKTVTIDRFFFDWRGGGRRGPSPV
                     DDVYESDAFADFRHSIARHDAVEGALDHAYWSDDAPCSMHIDEVEAIWSRIDESDDWS
                     LFDEKIGAIRRMGEALRAGA"
     gene            2452..>3710
                     /locus_tag="VGB57_04370"
     CDS             2452..>3710
                     /locus_tag="VGB57_04370"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEY0130619.1"
                     /translation="MATISSIEPATGAHLWTGEVGDVEAEVARARSAWAGWAARPLAV
                     RIETLRRFANVVRGHKEALSDLIARETGKPLWETATEVDAVVNKVDISVTAYSERTAQ
                     RRLEGALGARVAVRHKPHGVLAVLGPYNFPAHLPNGHIVPALLAGNAVVFKPSEKTPA
                     VGEMLVRLYHEAGVPEDVVRCVQGAVDIGKALAASPGIDGLLFTGSARAGLALHRQFA
                     DAPHKILALEMGGNNPLVVWDAKDISAAAVIAVQSAFMSSGQRCTGARRLIVQDGAHE
                     PLLAEIAKITGRIIVDHPHASPAPFMGPVIDNQAADQLQEAFLDLMIKGGRAIRRLDR
                     PVADKPFLTPAIIDVTDVAERPDAELFGPVLQVIRVPDFDAALAEANATRYGLAASLV
                     GGSPELYDRFWANIRAGVINWNKPTNGA"
BASE COUNT          572 a         1338 c         1205 g          595 t
ORIGIN      
        1 tgccagcctc gtcattccgg cgaaggccgg aatccatgaa caccgccgtt caacgtttct
       61 tcccacggtg ttcatggatc ccggatcaag tccgggatga cgcctctgcc ccagcgacga
      121 tccgcgccag cagccggtcg atccccgcca ccgcctcggg ctcctccaga gtcggcgcgt
      181 ggccgacatt ggggaccgtc gcggcctcca tgtccgggtt ctcggccagc atccgctcca
      241 ccgtcgcccg gctcagcagg tccgagatcc cgccgtggac gaccagcgac ggcaccccct
      301 tcaacgccgc gaacgcgctc cacaggtcga agccgctgtc gccccccggc tgccggaagg
      361 gctcggcgat tcgcatgtcg tagtcgaaga cgacccggcc gctccggttc agcctgcaaa
      421 gccttttggc gtaggccagc cactggccga tgtcccagtc cggatagcgg tcgctctgcg
      481 cctcgcaaag gaatcgcgcg gcgtggagcc aggtcggcca gctctgggag cgcccgacat
      541 aggagcggat atgctcgagc ccgccctgct cgagctccgg cccgatatcg ttgaggacgg
      601 cgccggcgat cctttccttg cctgccagtg ccagaagcat ggtgatgagc ccgccgagcg
      661 aggtcccgaa cagcacgaag cgcttcagct ccagagcggc gatcagcgcc tcgagatcct
      721 gccagtaggt gggcgggacg taggtcatcg gatccttcgc atggtcgctc tgcccgcgtc
      781 cgcgaagctc gacgcagatc agccgccact cgcccttcag tcggtcggca accccttcga
      841 agtcgcgcgc gttacgggtc agtccgggta tgcacaggat gggcgggcgg gccgcgcttc
      901 cggcataatc gcggtaatgg agccggagcc cgtcgctcga cgtccaatag ccatcggacc
      961 aatccgccat gcccctcccc tatttgcccg tctttcctat ctcccgatga cgcaggttcc
     1021 gcaagccgcg acctatcgcg ccgatcccgc catcctggat ctcggcgacg ccttttacga
     1081 cccggtggag gcggccgatt tcccgcagac cctgcttcgc ttccgcaacg accgctgggc
     1141 cccggcggtc gggctggagg cgcttggcgt tgaagagtgg ctccggcact tcggccggtt
     1201 cgagccgctg ccggacaatc tcccccggcc gctcgccctt cgctaccacg gccaccagtt
     1261 ccgagtgtac aatccggaca tcggcgacgg tcgcggcttc ctcttcgcac agcttcggga
     1321 cggcgacggc cggcttctcg acctcggaac caagggctcg ggccggactc cctacagccg
     1381 cttcggcgat ggccggctga ccttgaaggg cggcgttcgc gaggtgctgg cgaccgagat
     1441 gctggaggcg ctcggggtcg agacgtcgaa gaccttctcg ctgatcgaga ccggcgaggc
     1501 cctcgaacgg ggcgacgagc cctcgccgac ccggtcggcc gtgctggtac ggctccagca
     1561 cagccatatc cggatcggca ctttccagcg cctcgcctat ctccaggaga ccgggaatct
     1621 gacgaagctc accgattatt gcctgcgcca ttattacgga gacgaaggtg gtggggacga
     1681 cgctccggcc cgcctcctcg cccacgtggc gcgcgaggcc gcccggctcg cggcctccta
     1741 tatcgcggcc ggcttcgtcc atggggtgct caacagcgac aatatcgcaa tcaccgccca
     1801 gagcttcgac tatgggccgt ggcggttcac gccttattgg gacggacact tcaccgccgc
     1861 ctatttcgac catgccggcc tctatgcctt cggccggcag ccggaagcga tccactggga
     1921 tgtgcttcag ctcgccgtct cgcttcggac gatcgccgag gcggacgttc ttaacccggc
     1981 cctcgacgcc ttcccgcccg aattccagcg ccggctggtc gcggccatct tcggccggct
     2041 cggcatcagg ccgagcggcc aggaggagga ggacgccgct ctcctcgtcg cgctcgagag
     2101 ggcgctcgcg gcgaagacgg tgaccatcga ccgcttcttc ttcgactggc gcggcggcgg
     2161 gcgacggggc ccctccccgg tggacgatgt ttacgaaagt gatgctttcg ccgattttcg
     2221 ccactccatc gcacgtcacg acgcggtgga gggcgcgctc gaccatgcat attggtcgga
     2281 cgacgcgccc tgctcgatgc acatcgacga ggtcgaggcg atctggagcc gtatcgacga
     2341 gtcggacgac tggagcctct tcgacgagaa gatcggcgcg atcaggcgga tgggcgaggc
     2401 tttgcgggcg ggcgcttgac cgccgcagcc aaaaatggtg ggtcagccgc catggcgacc
     2461 atcagttcga tcgaaccggc caccggggcg catctgtgga cgggcgaagt cggcgacgtc
     2521 gaggccgagg tcgcccgcgc ccgctccgcc tgggcgggct gggcggcgcg gccgctggcg
     2581 gtcaggatcg agaccctgcg ccgcttcgcc aacgtcgtcc gaggccataa ggaggcgctt
     2641 tccgacctca tcgcccgcga gaccggcaag ccgctctggg agaccgcgac cgaggtcgac
     2701 gcggtggtca acaaggtcga catctcggtc acggcctatt cggagcggac cgcgcagcgg
     2761 cggctggaag gggcgctcgg cgcccgcgtc gccgtccgcc acaagccgca cggagtcctg
     2821 gccgtgctcg ggccgtataa tttcccggcc cacctgccca acggccacat cgtcccggcc
     2881 ctgctcgccg gcaatgcggt ggtgttcaag ccttcggaga agaccccggc ggtgggggag
     2941 atgctggtcc ggctctacca cgaggccgga gtccccgagg acgtcgtccg ctgcgtccag
     3001 ggcgcggtcg atatcggcaa ggcgctggcg gcgagccccg gcatcgacgg cctgctcttc
     3061 accggctcgg cccgcgccgg cctcgcgctc caccgccagt tcgccgacgc tccgcacaag
     3121 atcctcgcgc tcgagatggg cggcaacaac ccgctcgtcg tctgggacgc caaggacata
     3181 tcggcggccg cggtgatcgc cgtccagtcc gccttcatga gctccggcca gcgctgcacg
     3241 ggcgcccggc gcctgatcgt ccaggacggc gctcacgagc cgctgctcgc cgagatcgcc
     3301 aagatcacgg ggcggatcat cgtcgaccat ccccatgcga gccccgcccc cttcatgggc
     3361 ccggtcatcg acaaccaggc cgccgaccag ctccaggaag cctttctcga cctgatgatc
     3421 aagggcggca gggcgatccg gcggctcgac cgtccggtcg cggacaagcc gttcctcacc
     3481 ccggcgatca tcgacgtcac cgacgtcgcc gagcgccccg acgcggagct gttcgggccg
     3541 gtgctccagg tcatccgcgt ccccgatttc gacgccgcct tggcggaagc caacgcgacg
     3601 cgctacggcc tggccgcctc gctcgtcgga ggaagcccgg agctttacga ccgcttctgg
     3661 gcgaatatcc gggccggcgt gatcaactgg aacaagccga caaacggcgc
//
LOCUS       DASWNU010000224         6136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_9143,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000224 DASWNU010000000
VERSION     DASWNU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9143"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGB57_04375"
     CDS             complement(<1..270)
                     /locus_tag="VGB57_04375"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEY0130620.1"
                     /translation="MERRDHLEAIWRAGVEACLPERVLPPHLPPPPPGRTLLLALGKA
                     AVPMARAVETRWQGPLSGLAVTPHGSGGMLERIAILPAAHPVPDES"
     gene            413..1138
                     /locus_tag="VGB57_04380"
     CDS             413..1138
                     /locus_tag="VGB57_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HEY0130621.1"
                     /translation="MKRASRIAFRAAGALVAIPILYFLAALILGAVPANRGWEEAREG
                     VTVFVRTNGVHTWILVPKVTPEMDWRPLVPGGHLRDRRWGQGNYVALGYGNRTFYLET
                     PTWGDLTMKNAFLAAFGQGRSLMHADHDHDPRASDDTRPIVLRSEEYRRLVEFARASF
                     QLDAQGRTMPILGRGYGTSDMFYEAVGPYSAVHTCNSWTGAALRRAGVKTGVWTPLSQ
                     SIMWRIASPSETGRGTPRSGVEG"
     gene            1288..1878
                     /locus_tag="VGB57_04385"
     CDS             1288..1878
                     /locus_tag="VGB57_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY0130622.1"
                     /translation="MAKILGNFHLALVIGVVLTIAAMFGLHGGLIGDGSYDWTQFMRY
                     LHIFAGILWIGLLYYFNFVQIPTMPKVPAELKPGVSKYIAPTALFWFRWAALATVILG
                     LLTGEMKEGGYTARALGLAETHRTIGIGMWLGIIMAANVWFVIWPNQKRALGIVPAAD
                     DVKARSASVAMMASRTNLILSLPMLYCMVLYAHLSA"
     gene            2006..5611
                     /locus_tag="VGB57_04390"
     CDS             2006..5611
                     /locus_tag="VGB57_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0130623.1"
                     /translation="MTKGWSFSIDRGGTFTDIVARPPDGRLLTAKLLSDNPEQYEDAA
                     VEGIRRLVAEHGEAPLEAVKMGTTVATNALLERKGEKLVLAVTRGLGDALRIGYQARP
                     DIFARQIVLPEALYTDVVEVDERVTAEGEVLRPLDLEDARASLEGAYEAGYRAIAIVL
                     MHGWRWTAHEAALAALAEEIGFTQVSASHRVGPLIKLVGRGDTTVVDAYLSPVLRRYV
                     DRVVRALGSGARLFFMQSSGGLTDAAAFQGKDAILSGPAGGIVGMARTAEEAGFDRVI
                     GFDMGGTSTDVSHYAGAYERTNETVVAGVRVRAPMLEIHTVAAGGGSVCHYDGARFRV
                     GPDSAGAVPGPACYRRGGPLTITDCNVVLGKIRPEHFPRVFGPGGEEPIDAAASRALC
                     EAIGAEAGMSTEEAAAGFVRIAVDNMASAIKRISIARGHDVTRYTLACFGGAGGQHAC
                     LVADALGMERVMIHPLAGVLSAYGMGLADMVELRQRSVGDEALEDVLAELAGEAEAAL
                     KGQGVEGVELKRRAALRYEGSDSSLEVEAGEFEAMREAFEEAHRTRFGFLSPETPVVV
                     ETAIVEASSSPSPDGEGDHAKHGGGVAGADLFHLPLHHPSGGPPPHAAHGEDLFDRSQ
                     LSPGTIIPGPALIIDPSATTVVEPGWQAEVDKLANLILTRSTPRQTSHAIGTSADPAM
                     LEVFNNLFMAVAEEMGVALQSTASSVNIKERLDFSCALFDSGGALIANAPHIPVHLGS
                     MGDSIRTVIDARGEGRDGRGLCEGDVYVLNAPYRGGTHLPDITVIMPVFAGAGDPVPA
                     WFVAARGHHADIGGIAPGSMPPDSRTIADEGVLIDNALLVDRGRFLETEIRALLASGP
                     WPARRPDRNISDLKAQVAACARGAAEMRRVAGEYGPGVVDAYMAHVQANAEEAVRRLI
                     GGLSDGDFRYAMDNGAEVCVSVKVEPASRSAIVDFTGTSGQQPNNFNAPYSICRAATL
                     YVFRTLVDDSIPMNDGCLRPITLIVPEGSMLNPRYPAAVVAGNVETSQVITDALFAAC
                     RALAPSQGTMNNFTFGDDQHQYYETIAGGAGAGPDFDGASAVQTHMTNSRLTDPEVLE
                     TRYPVLVERFAIRRGSGGEGAHKGGDGVERHIRFRTRMRANILSNRREVAPRGIEGGG
                     DARPGVNQVVRADGTVETLSATASAEMREGDMFVIETPGGGGYGPPRHSGESRNP"
     gene            5679..>6136
                     /locus_tag="VGB57_04395"
     CDS             5679..>6136
                     /locus_tag="VGB57_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 family protein"
                     /protein_id="HEY0130624.1"
                     /translation="MNYLPLLGILIIILGFALRFNPMVVVPVAAIATGLLAGLDPVEV
                     ISKLGKAFNDSRLIAVPWIVLPVIGLLERYGLQQRARAVISGFRAATAGRLLILYLFY
                     RQIISAIGLHSTAGHPQTVRPLVAPMAVAAAEGAGQLDEATSDKVKAYAAA"
BASE COUNT          979 a         2140 c         2048 g          969 t
ORIGIN      
        1 gctctcgtcg ggcacggggt gggcggccgg caggattgcg atccgctcga gcatcccacc
       61 gcttccgtgg ggagtcaccg ccagtcccga cagcgggccc tgccatctcg tctcgaccgc
      121 ccgcgccatc ggcaccgccg ccttgcccag cgcgagcaga agggtgcggc cgggtggcgg
      181 cggcggcaga tgcggcggaa gcacccgctc gggcaggcac gcctccaccc ccgcccgcca
      241 gatcgcctcg agatggtcgc gccgctccat cgaccgagct tggcggctcg cgcgaacggg
      301 ctcaaccccg ctcccttggg gagaaagacg ttcaaagact tagaaatcgg tgccgggcac
      361 caatttggga tcgagggctt cccagcgccg gcggttgcgc taggctgtcc ccgtgaaacg
      421 cgcctcccgc atcgcctttc gcgccgccgg cgcattggtt gcgatcccga tcctctattt
      481 cctcgccgcc ctgatcctcg gcgccgtccc cgccaatcgc ggctgggagg aagccaggga
      541 gggggtgacc gtcttcgtcc gcaccaacgg ggtccatacc tggatcctgg tgcctaaggt
      601 cacgcccgag atggactggc gcccgctcgt ccccggcggc catctcaggg accggcgctg
      661 ggggcagggc aattacgtcg ctctgggcta tggcaaccgc accttctatc tcgagacccc
      721 gacctggggc gacctcacca tgaagaacgc cttcctcgcc gccttcggcc agggccggag
      781 cctgatgcac gccgatcacg accatgatcc gcgggcgagc gacgacacga ggccgatcgt
      841 ccttcggagc gaggaatatc gccggctcgt cgagttcgcc cgggccagct tccagctcga
      901 cgcgcagggg cgcacgatgc ccatcctcgg ccgcggctat gggacgtccg acatgttcta
      961 cgaggcggtg ggcccgtaca gcgccgtcca cacctgcaat agctggaccg gagcggccct
     1021 ccgccgcgcc ggggtgaaga cgggcgtgtg gaccccgctg tcgcagagca tcatgtggag
     1081 aatcgcctcc ccatcggaga cggggagggg gaccccgcgg agcggggtgg aggggtaatt
     1141 ggcgcaggcc tgttccagcc acccctccac catgcttcga atggtccccc tccccacgag
     1201 cttcgctcgc agggaggcaa ggttcgcact acccacgcct tcgccgctgc gttaaggcgg
     1261 cagcaaatca aaccacaggg ggtttcgatg gcgaagatac tcggcaactt ccatctggcg
     1321 ctggtgatcg gcgtcgtgct gacgatcgcg gcgatgttcg ggctgcacgg cgggctgatc
     1381 ggcgacggca gctacgactg gacgcagttc atgcgttacc tccacatctt cgccgggatc
     1441 ctgtggatcg gcctgcttta ttatttcaac ttcgttcaga tcccgaccat gcccaaggtg
     1501 ccggcggagc tgaagcctgg cgtgtccaaa tatatcgctc ccacggcgct cttctggttc
     1561 cgctgggcgg cgctggccac cgtgatcctc ggccttctca ccggcgagat gaaggaaggc
     1621 ggctacaccg cgcgcgccct cggcctcgcc gagacccacc gcaccatcgg catcggcatg
     1681 tggctcggga tcatcatggc ggcgaacgtc tggttcgtga tctggccgaa ccagaaaagg
     1741 gcgctcggca tcgtgcccgc ggccgacgat gtgaaggcgc gttccgcctc ggtggcgatg
     1801 atggcctcgc gcaccaacct catcctgtcg ctcccgatgc tctactgcat ggtcctctac
     1861 gcccacctca gcgcctgaac gctaagcgcc gccgcggatg accttcacct ccgtcattgc
     1921 gaggagcgaa gcgacgaagc aatccagcct ccgatctccg ggccgctgga ttgcttcgct
     1981 tcgctcgcaa tgacggaggt agcaaatgac gaaaggctgg tccttctcga tcgaccgcgg
     2041 cggcaccttc accgacatcg tcgcccgccc gcccgacggc cggctgctca ccgccaagct
     2101 gctctccgac aatcccgagc aatatgaaga cgccgccgtc gaaggcataa ggcggctggt
     2161 cgcggagcat ggcgaggccc cgctcgaagc ggtgaagatg ggcaccaccg tcgcaaccaa
     2221 cgccttgctc gagcgcaagg gcgagaagct cgtcctggcg gtcacccggg gcttggggga
     2281 cgcgctcagg atcggctacc aggcgcgacc ggacatcttc gcccgccaga tcgtcctgcc
     2341 cgaggcgctc tacaccgacg tcgtcgaggt ggacgaaagg gtgaccgccg agggcgaagt
     2401 gcttcgtccg ctcgacctgg aggacgcccg cgcctcgctt gaaggcgctt atgaggcagg
     2461 ctaccgcgcc atcgccatcg tcctgatgca cggctggcgc tggaccgctc acgaggcggc
     2521 gctggcggcg ctggccgagg agatcggctt cacccaggtg tcggcgagcc accgcgtcgg
     2581 gccgctgatc aagctggtcg ggcgcggcga cacgacggtg gtcgacgcct atctctcgcc
     2641 ggtccttcgc cgctatgtcg accgggtggt ccgtgcgctt ggaagcggag cgcggctctt
     2701 cttcatgcaa tcgtcgggcg gcctcaccga cgccgccgcc ttccagggca aggacgccat
     2761 cctttccggc ccggccgggg gaatcgtcgg catggccagg accgccgagg aagcggggtt
     2821 cgaccgggtc atcggcttcg acatgggcgg cacctcgacc gacgtctccc attatgccgg
     2881 cgcttacgaa agaaccaacg agacggtcgt ggccggagtc cgggtccggg caccgatgct
     2941 cgagatccac accgtcgcgg cgggcggcgg ctcggtctgc cattatgacg gcgcgcgctt
     3001 ccgggtcggc cccgacagcg ccggggcggt gccggggccg gcctgctatc gccgcggcgg
     3061 gccgctcacc atcaccgact gcaacgtcgt cctcggcaag atccggcccg agcatttccc
     3121 ccgagtcttc ggaccgggcg gcgaggaacc catcgacgcg gcggcctcgc gggcgctttg
     3181 cgaggcgata ggcgccgagg cgggaatgtc aacggaggaa gccgccgcgg gcttcgtccg
     3241 gatcgccgtc gacaatatgg ccagcgcgat caagcggatc tccatcgccc ggggccacga
     3301 cgtcacccgc tacaccctgg cctgcttcgg cggcgccggc ggccagcatg cctgcctcgt
     3361 cgccgacgcg ctcggcatgg agcgggtgat gattcatccg ctcgcgggag tgctcagcgc
     3421 gtacgggatg gggctggccg acatggtcga gctgaggcag cgcagcgttg gggacgaggc
     3481 gctggaggat gtgctcgcgg agctggccgg ggaggcggag gcggcgctga aggggcaggg
     3541 ggtcgagggg gtcgagctga agcggcgggc ggcgctgagg tatgagggga gcgattccag
     3601 cctggaggtg gaggcgggcg aattcgaggc gatgcgggag gcgttcgagg aagcgcaccg
     3661 gactcggttc gggtttctgt cgccggaaac tccggtggtg gtcgaaacgg cgatcgtcga
     3721 agcctcatcc tccccatcgc cagatgggga gggggaccat gcgaagcatg gtggaggggt
     3781 agctggagca gacctgttcc acttacccct ccaccaccct tcgggtggtc cccctcccca
     3841 tgctgcgcat ggggaggatc tgttcgaccg ctcccaactc tcccccggca caatcatccc
     3901 cggcccggcc ctcatcatcg acccctccgc caccaccgtg gtggagccgg gctggcaagc
     3961 cgaagtggac aagctcgcca acctcatcct cacccgctcc accccccgcc aaacctccca
     4021 cgccatcggc acctcggccg atccggccat gctcgaagtg ttcaacaatc tgttcatggc
     4081 ggtcgccgag gagatggggg tcgcgctcca gagcactgcg agctcggtca acatcaagga
     4141 gcggctcgat ttctcctgcg ccctgttcga ttccggaggc gccctcatcg ccaacgcgcc
     4201 gcacatcccc gtccatctcg gctcgatggg cgattcgatc cgcaccgtca tcgacgcccg
     4261 cggagagggc agggacgggc gcggcctgtg cgagggcgac gtctacgtcc tcaacgctcc
     4321 ctatcgcggc ggcacccatc tgcccgacat caccgtgatc atgccggtct tcgccggggc
     4381 gggcgatccg gtgccggcct ggttcgtcgc cgcccgcggc caccacgccg acatcggcgg
     4441 catcgctccg ggctcgatgc cgccggacag ccgcaccatc gccgacgaag gcgttctgat
     4501 cgacaacgcg ctgctggtcg acaggggccg cttcctcgag acggagatcc gcgccctgct
     4561 cgcctccggc ccgtggcccg cgcgaaggcc cgatcgcaac atctccgact tgaaggccca
     4621 ggtcgccgcc tgcgcccgcg gagcggcgga gatgcggcgg gtggccggcg aatatggccc
     4681 aggtgtcgtc gacgcctaca tggcccatgt ccaggccaat gccgaggagg cggtccggcg
     4741 gctgatcggc ggcttgagcg acggcgactt ccgctacgcg atggacaatg gcgccgaggt
     4801 ctgcgtctcg gtcaaggtcg aaccggcgag ccgctcggcg atcgtcgact tcaccggcac
     4861 cagcggccag cagccgaaca atttcaacgc gccttattcg atctgccgcg ccgcgacgct
     4921 ctacgtcttc cggaccctgg tcgacgactc gatcccgatg aacgacggct gccttcggcc
     4981 gatcaccctg atcgtccccg aaggctcgat gctgaacccg cgctacccgg ccgccgtcgt
     5041 cgccggcaat gtcgagacca gccaggtgat caccgacgcc ctgttcgcgg cctgccgggc
     5101 gctcgctccg agccagggga cgatgaacaa tttcaccttc ggcgacgacc agcaccaata
     5161 ttacgagacg atcgccggcg gcgccggggc cggcccggat ttcgacgggg cgagcgcggt
     5221 ccagacccat atgaccaaca gccgcctgac cgatccggaa gtgctcgaga cccgctatcc
     5281 cgtcctggtc gagcgcttcg ccatccgccg cggcagcggc ggcgaaggcg cgcacaaggg
     5341 cggcgacggg gtcgagcgcc acatccgctt ccggacaagg atgcgcgcca atatcctctc
     5401 caaccgacgc gaggtggcgc cgcgcgggat cgagggggga ggggacgcca ggcccggagt
     5461 aaaccaggtg gtccgggccg acggcaccgt cgagactttg agcgcgacgg catcggcgga
     5521 gatgcgggag ggggacatgt tcgttatcga gaccccgggg ggagggggct atggccctcc
     5581 ccgtcattcc ggcgaaagcc ggaatccatg aacaccgccc ttttcgcaaa accccgtgtt
     5641 catggatccc gggtcaagcc cgggatgacg ggggctcgat gaactacctc ccgcttctcg
     5701 gcatcctcat catcatcctc ggcttcgcgc tccgcttcaa cccgatggtg gtggtgccgg
     5761 tggcggcgat cgcgaccggg ctgctcgcgg ggctcgaccc cgtcgaggtg atatcgaagc
     5821 tcggcaaggc gttcaacgac agccgcctga tcgccgtgcc gtggatcgtt cttcccgtca
     5881 tcggcctgct cgagcgctac ggcctccagc agcgggcgag agcggtcatt tccggatttc
     5941 gcgccgcgac agcaggccgg ctgctcattc tttatctgtt ctaccgccag atcatctcgg
     6001 cgatcggcct tcattctact gcagggcatc cgcagacggt tcggccgctg gtcgcgccga
     6061 tggcagtcgc cgccgccgaa ggcgccggcc aactcgacga ggcgacaagc gacaaggtca
     6121 aagcctatgc ggccgc
//
LOCUS       DASWNU010000225         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_267777, whole genome shotgun sequence.
ACCESSION   DASWNU010000225 DASWNU010000000
VERSION     DASWNU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267777"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VGB57_04400"
     CDS             <1..927
                     /locus_tag="VGB57_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0130625.1"
                     /translation="ASEALQRLAAYESDIILAQLALLEANEAAAERGRSSDAFERDVA
                     GLVEATTGESARLREKTGRTASSARGMLGKTAEVAEAAEQSALAMRDAAETAAGLIRA
                     IEDARQEVEVAAGVAVRAGEEASQATRASRALSGHVEAIESILGLIRDIAGQTNLLAL
                     TATIEAARAGDAGRGFAVVAQEVKSLAAQTARATDDITGKISAIQQSTRQTVEANGSV
                     QKTVEDVQASAERIREAMEVQARTVTMITAAVDETALAADSMSGTIAAIRHDTENVAS
                     DIDSVESGFANVDDRLTRFRAATAEFVTGFAA"
     gene            1034..1561
                     /locus_tag="VGB57_04405"
     CDS             1034..1561
                     /locus_tag="VGB57_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130626.1"
                     /translation="MDPKRIARLTEQQRACLRHVYAHLTSKEIAPILGIAPGSVDQHI
                     KAAMKTLGVADRRTAAIVLAEHEGREDPPVVAYSSPDPASPTDPYNFVPPIVSGWQPH
                     ESAGASMREEQAFFRVATPVGSPTFQFPIGSSKPGDLNWLARLAWIAAIAIGVALAFG
                     ALVSAIEALGRLTQG"
BASE COUNT          299 a          595 c          576 g          244 t
ORIGIN      
        1 gcgagcgagg cgctgcagcg actcgccgcc tatgaaagcg acatcatcct cgcccagctc
       61 gccttgctcg aagccaatga ggcggcggcc gagcgcggtc gaagcagcga cgcgttcgag
      121 cgcgacgtcg ccggtctggt cgaggcgacc acgggcgaat cggcgaggct tcgcgagaag
      181 accggccgga cggcctcctc cgcccgcgga atgctcggca agaccgcgga ggtggccgag
      241 gccgccgagc aatcggcgct ggcgatgcgc gacgccgccg agaccgccgc cgggctcatc
      301 cgggctatcg aggatgcgag gcaggaagtg gaggtcgccg ccggggtcgc cgtccgcgcg
      361 ggcgaggaag cgagccaggc gacgcgggcg agccgggcgc tgtcgggcca tgtcgaggcg
      421 atcgaatcga tcctcggcct gatccgcgac atcgccggcc agaccaacct cctcgccttg
      481 accgccacca tcgaggcggc ccgggccggc gatgccggcc ggggcttcgc cgtcgtcgcc
      541 caggaagtga agtcgctcgc cgcccagacg gcccgcgcca ccgacgacat caccggaaag
      601 atcagcgcca tccagcagtc gacccgccag accgtggagg ccaacggctc ggtccagaaa
      661 accgtcgagg acgtccaggc atccgccgaa cggatccgcg aggcaatgga ggtccaggcc
      721 cggaccgtca ccatgatcac cgccgcggtg gacgagaccg cgctcgccgc cgattcgatg
      781 tccgggacga tcgcggcgat tcgccacgac accgagaatg tcgccagcga tatcgactcc
      841 gtggagagcg gattcgccaa tgtcgacgac cggctgaccc gcttccgcgc cgccaccgcc
      901 gagttcgtca cgggattcgc cgcctgacgt cgggtgaaac caaccggcgg tccgttcgtt
      961 ggatgtgcct gaattgcggg ctctacctgc cccattgtgc agaagcccgt cctaagcgat
     1021 aatcccattc cgcatggacc cgaaacgcat cgccaggctg accgagcaac agcgtgcctg
     1081 cctcaggcat gtctacgcac atctgacgtc caaggagatc gctccgatcc tcgggatcgc
     1141 gcccggctcc gtcgaccagc acatcaaggc ggcgatgaag actctcggag tcgccgaccg
     1201 ccgaaccgcc gccatagtgc tcgccgagca tgaagggcgc gaggatccgc ctgtggtggc
     1261 atattcgtcc ccggaccctg cctcgccgac cgatccgtac aatttcgtgc cgccgatcgt
     1321 gagcggctgg cagccgcacg aatcggccgg cgcatccatg cgggaggagc aggccttctt
     1381 ccgagtcgcc acgccggtcg gcagtccgac tttccagttc ccgatcggct cgtccaagcc
     1441 gggcgacctg aactggttgg cgaggctggc gtggatcgcg gcgatcgcca tcggggtggc
     1501 actggccttc ggagccctgg tctccgcgat cgaggcgctc ggcaggctga cccagggctg
     1561 agcaatttgc cttaagctgc cctgcggact agcgagcccg ccgaatcgag cttctcgtgc
     1621 aggctatggg cggaaaattc caggccgttg ggccaagcca gcgctcccgc gtcgataaat
     1681 gcgcgtcgga aataggcggg gtcttcgagc ggac
//
LOCUS       DASWNU010000226         6384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_371207, whole genome shotgun sequence.
ACCESSION   DASWNU010000226 DASWNU010000000
VERSION     DASWNU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371207"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..622
                     /locus_tag="VGB57_04410"
     CDS             140..622
                     /locus_tag="VGB57_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130627.1"
                     /translation="MRNIPTLPKPGRRAALALLLALPAGCAIDGPVRDKDYSACRVLG
                     SSDWKAQVEFSSTSSPIPYLTRKLVVTGKVTTAAGYYASLAEGPVARLDEPIQQVRVR
                     TEGAAQPGGAPVVHNVRGVFPAIERYGGVAIRCGDGIIAEIREVPVPPRQKRENPFSF
                     "
     gene            complement(619..1023)
                     /locus_tag="VGB57_04415"
     CDS             complement(619..1023)
                     /locus_tag="VGB57_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0130628.1"
                     /translation="MTTELDVLAWGCILALVHIFVAVRFKTRQYGTKWNVGARDEELP
                     PPRPIVGRLARAQANFFETFPIFAAAALVVAVAGLGDRWTAIGAWLWLGARIVYLPLY
                     ALGVPVVRTIAFLISVVGILLVLRPALAASFG"
     gene            1379..2551
                     /locus_tag="VGB57_04420"
     CDS             1379..2551
                     /locus_tag="VGB57_04420"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0130629.1"
                     /translation="MPSPHLIAAPEPDLSLPGWIYRDREFFEIEMERVIRPSWQAVCH
                     AGEIPAPGDWRTLDYLGESILVIRGDDSIVRAFSNVCRHRGSRVVSGGSGCARRLTCP
                     YHAWTYALDGRLIGVPNRSDYPRLETSKLGLAPVELEAWRGFLFVRLEGGGPSVAEMM
                     APHEAEIEPYRFEALEALGRVTLRPRPVNWKNVADNYSDGLHIAVAHPGLTRLFGRGY
                     AISAGEHVDRMAGDLIERPSANPSERAYQKHLPPVPHLPAELQRRWLYFKLWPNVAFD
                     IYPDQIDFMQFLPVSATQTVIREISYAVPDANMPERWRREMKAARYLNWRINRRVNAE
                     DTALIEGVQQGMASRSYVPGPLGESEVCLRSFARKLRRLIPEANLHQPPKAGWSRR"
     gene            complement(2656..3063)
                     /locus_tag="VGB57_04425"
     CDS             complement(2656..3063)
                     /locus_tag="VGB57_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEY0130630.1"
                     /translation="MRLKTLTYTSRARLDLGEEDLAAIHQTARHLNALDGVSGLLLFD
                     GSRFLQIIEGAEDAIDNLVERLRMDSRHSAFEVRDERYVERRSFPDWSMELVRVSAGY
                     GNAKEEVATALPDAVAAPVRELVLKMTGRLAEA"
     gene            complement(3113..3349)
                     /locus_tag="VGB57_04430"
     CDS             complement(3113..3349)
                     /locus_tag="VGB57_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEY0130631.1"
                     /translation="MRAARLSSSVIARVAYDDEERALSIWFRETGRYIYSDVPKAIYE
                     ALCKAPSAGRFYNECIKRRFPCRPDPERRKFRPA"
     gene            3532..>6384
                     /locus_tag="VGB57_04435"
     CDS             3532..>6384
                     /locus_tag="VGB57_04435"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0130632.1"
                     /translation="MSTGARAALLLLLALLALPATAAGQSNPHLTALVGGVDDEDNRR
                     DWLRIEKLGLEIRIHGGIADTTLTARISNGGDERVEADFGLALPPGSTVTGYALDVDG
                     DLVEGVLVDQRKARQAYQERVRQDVDPGLGEVLRGYQFRTRISPVLPGDRRTVRIHFS
                     TPLDPRLGYVLPLTGGAVVGELDITVEASGLSRAPAVRLPDGGRARSESRSGTWRFSY
                     SGGQVRLGGDLSVSEVERSSPLLSSNRPNGDSYFELSDRGDSSAPPGRPRSVAVLWDR
                     SLSRADDALGAEAALLKAYLERARPDRIELIFFDASLVERVAVATPEAAAAAVAGLRY
                     RGASSFALLSSHPIEAEACLLFSDGLVTIDRRDSFRPACSLFAVSSAPDSDRAWLSAI
                     AQASGGSALQLGPGNSEQLLDRLAGSTPRVLAVRTAAGAPIDFTLLDAPEKGWRLVGP
                     LPSSRSVVVRLAGPNGRPIERVYATGEGEAPRLNGAGALWASDRLALKVANDDSDRDD
                     IVAFARRHRVAAPDVSFIVLERPADYARAGIEPPLAYAASKRAEFDRLLAEKLADERR
                     DQEARLATVIEAWESQKAWWKKRFDPDAKTNKGKENAAPPPPPPAPSAPPQPVTAQPP
                     AEEPAPDQLNELPQLRSTNDDAAEGADITVTGTRIRNPNLDSMAPVTSIGGEEFFQRG
                     NAEAEPAKAAIELPEWTADRPYLKALAAARASELETVLAREQAEHGALPAFWLDVSDW
                     YFRAGRRPEALRLLLSALDLPTRNSETVAVVAARLVRWGELDRAVQLFERLAAAESDR
                     PQPLRSLALALAKRAEAGPSPQSRADLARAMALLTQVVMTPWGEDYDGMEMISLMEVN
                     RLIPRYRKLGGADVPLDPRLIALLDVDLRVTIEWNTEETDLDLWVDEPNGERAMYSNP
                     ATAIGGRLSNDMTNGYGPEEYLLRRAPDGTFTVRADV"
BASE COUNT         1014 a         2242 c         2150 g          978 t
ORIGIN      
        1 cacggcgcat ctggtctgct tcggggcgat ctgggtcgcg ctcgccatct tcgtcggcga
       61 aggcgtccgc gcccaccgcg ccgcggccgc cgaggcagcg gtctagccgc cccgccgggg
      121 acccgctaag gcacgggcga tgcgtaacat cccgaccctt ccgaagcccg gccgccgggc
      181 cgccctggcc ctgctcctcg ccctccccgc cggctgcgcg atcgacgggc cggtgcgcga
      241 caaggattat tcggcctgcc gggtgctggg ctcgtcggac tggaaggcgc aggtcgagtt
      301 ctcgtcgacc tcgagcccga taccctatct gactcgcaag ctcgtggtca ccggcaaggt
      361 gacgacggcg gcaggctatt atgccagcct cgcggagggg ccggtcgccc ggctcgacga
      421 gccgatccag caggtgcggg tgcggaccga gggcgcggcc cagccgggcg gggcacccgt
      481 cgtccacaac gtccgcggag tcttcccggc gatcgagcgc tacggcgggg ttgcgattcg
      541 ctgcggcgac gggatcatcg ccgagatccg cgaggttcca gtcccgccgc gccagaagcg
      601 cgagaatccc ttctccttct agccgaagga cgccgcgagc gcggggcgaa gcaccagcag
      661 gatgccgacc accgagatca aaaaggcgat cgtccggacc accggaacgc ccaaagcgta
      721 gagcggcagg tagacgatcc gggcgccaag ccacagccag gcgccgatcg ccgtccagcg
      781 gtcgccgagc ccggcgacgg cgacgaccag cgccgcggcc gcgaagatcg ggaaggtttc
      841 gaagaaattg gcctgggcgc gggcgaggcg gccgacgatc ggccggggcg gcggcaattc
      901 ctcgtcgcgg gcgccgacat tccatttcgt cccatattgg cgggtcttga agcggaccgc
      961 gacgaagatg tggacgaggg cgagaatgca gccccaggcg aggacatcga gctcggtggt
     1021 catggaggcg gttgtccggc agtcggaacg aatgcgcaag gccctccatc gaagcaggct
     1081 tgccggatgg ggagcggcgg tgtcgccccc ggggccggcg gcccgacagc gacaaaggcg
     1141 acagctaagt ctctaagttt cggcttccca tggccctgaa aaagcggacc cggatcgagc
     1201 tgccgctgtc gccgttcggc gagactcgaa ggagaaggca ggggcaaatc cacgtccgga
     1261 acataacatg aaaacatcat tccgccaaac ccgcccggcc gccttcggtt tcgactccgc
     1321 ggcaatgacc ggggagggcc gcaggtgaca ggagctgccg attttgccac aatcggccat
     1381 gcccagcccg cacctcatcg ccgcgcccga gccggatctc agcctgcccg gctggatcta
     1441 tcgcgaccgc gagttcttcg agatcgagat ggagcgggtg atccggccgt cctggcaggc
     1501 ggtctgccac gccggcgaga ttccggcgcc cggcgactgg cggacactcg actatctcgg
     1561 cgagagcatc ctcgtcattc gcggcgacga ctcgatcgtc cgcgccttct cgaacgtctg
     1621 ccgccaccgc ggctctcgcg tggtgagcgg cggatcaggc tgcgcgaggc ggctgacctg
     1681 cccctatcat gcctggacct acgcactgga cggccggctg atcggggtgc cgaacagaag
     1741 cgactatccg aggctggaga cgtcgaagct cggactcgcg ccggtcgagc tcgaggcctg
     1801 gcgcggcttc ctcttcgtcc ggctcgaggg cggcggtcct tcagtcgcgg agatgatggc
     1861 gccccacgag gcggagatcg agccctaccg gttcgaggcc ctggaggcgc tcggccgggt
     1921 cacgctccgg ccgcgccccg tcaactggaa gaatgtcgcc gacaattatt cggacggcct
     1981 ccacatcgcc gtcgcccatc ccggcctcac ccggttgttc ggccgcggct atgccatctc
     2041 ggcgggcgag catgtcgacc ggatggccgg cgacctgatc gagcggcctt ccgccaatcc
     2101 gtccgagcgc gcctatcaga agcatcttcc gccggtcccg cacctgccgg ccgagctcca
     2161 gcggcgctgg ctctatttca agctctggcc caacgtcgcc ttcgacatct atccggacca
     2221 gatcgacttc atgcagttcc tgccggtctc ggcgacgcag accgtgatcc gcgagatcag
     2281 ctatgcggtg cccgatgcca atatgccgga aaggtggcgc cgcgagatga aggcggcccg
     2341 ctacctcaac tggcggatca accggcgggt caatgcggag gacacggctc tgatcgaggg
     2401 cgtccagcag ggcatggcct cgcgaagcta cgttcccggc ccgctcggcg agagcgaggt
     2461 gtgcctgcgc agcttcgctc gaaagctcag gcggctgatc ccggaagcga atttgcacca
     2521 gccgccgaag gcgggctgga gccggcgtta gtcgtcattc cggcgaaagc cggaatccat
     2581 aaacaccgcg gcgcgagttg cttccagctg ccgtgttcat ggatcccgag tcgagcccgg
     2641 gatgacgata cagcctcaag cctcggcgag ccggccggtc atcttgagga ccagctcccg
     2701 caccggcgcg gcgacggcat ccgggagggc cgtcgcgacc tcctccttcg cattcccgta
     2761 gcccgcgctc acccggacaa gctccatcga ccagtccgga aacgaccgcc gctccacata
     2821 gcgctcgtcc cgcacctcga aggccgaatg gcgggagtcc atgcgcagcc gctcgaccag
     2881 attgtcgatc gcgtcctccg cgccttcgat gatctgaagg aagcgcgagc cgtcgaagag
     2941 gaggaggccg ctgactccgt ccagcgcgtt caggtgccgc gcggtctggt ggatcgcggc
     3001 caaatcctct tcgccgagat cgaggcgggc gcggctggtg taggtcaagg tcttcagacg
     3061 caccgctatt ctcctgaccg tccactggca cgatgggcgg cgccgatcaa cgtcacgccg
     3121 gccggaattt cctccgttcc ggatcgggtc ggcaggggaa gcggcgcttg atgcattcgt
     3181 tgtagaagcg gcccgccgag ggcgccttgc acagagcctc gtaaatcgcc ttcggaacgt
     3241 ccgaatagat gtagcgcccg gtttccctga accagatgct cagcgcccgc tcctcgtcgt
     3301 cataggcgac ccgggcgata acgctcgacg acagtctggc ggcgcgcatg atcctctcca
     3361 cgaaagagaa tcgaatgcca gcgcttatcg gaatcgcctg ttccggcggc ttcggcggac
     3421 cgaatcgata aagggccgaa gcgagtctcg cgcggcccct tggcgcccgg cccgccccgg
     3481 cctaggctgc gcccgaccgg ggggtgggag cagatgagtc attacttgcg catgagcacc
     3541 ggcgctcgcg ccgcgctcct tctccttttg gccctcctcg cgctgcccgc cactgccgcc
     3601 gggcagtcca acccccacct cacggccttg gtcggcgggg tcgacgacga ggacaaccgc
     3661 cgcgattggc tgcgcatcga gaagctcggg ctcgagatcc ggatccatgg cgggatcgct
     3721 gacaccaccc tgaccgcccg catctcgaac ggcggggacg agcgcgtgga ggccgacttc
     3781 gggctcgccc tgccgccggg ctccaccgtc accggctacg cgctcgacgt agacggcgac
     3841 ctcgtcgaag gcgtcctcgt tgaccagcgc aaggcgcggc aggcctatca ggagcgggtc
     3901 cggcaggacg tggaccccgg cctcggcgag gttcttcggg gttatcagtt ccgaacccgg
     3961 atcagcccgg tgctcccggg cgaccgccgc acggtgcgga tccacttctc gaccccgctc
     4021 gatccgcggc tcggctatgt gcttccgctc accggaggcg ccgtcgtcgg cgagctggac
     4081 atcacggtgg aggcaagcgg cctctcccgc gcgccggcgg tgaggcttcc ggacggcggc
     4141 cgcgcgcgat cggagagtcg ctccggcacc tggcgcttca gctattccgg cggacaggtc
     4201 cggctcggcg gcgatctgag cgtcagcgaa gtcgagcggt cctcgcccct cctctcctcg
     4261 aatcggccaa acggcgattc ctatttcgag ctttccgacc ggggggatag ttcggcaccg
     4321 ccgggcaggc cgcgcagtgt cgccgtgctc tgggaccggt ccctgtcgcg ggccgacgac
     4381 gctctcggcg ccgaggcggc gctcctcaaa gcctatctgg agcgggcccg gccagaccgg
     4441 atcgagctga tcttcttcga cgccagcctc gtcgagcggg tggccgtcgc cacgcccgag
     4501 gcggcggccg ccgccgtcgc cgggctgcgc tatcgaggcg cgtcgagctt cgcgctgctg
     4561 tcgagccacc cgatcgaggc cgaagcgtgc cttctcttct ccgacggcct ggtgacgatc
     4621 gaccgccgcg actcgttccg gccggcctgc tccctattcg ccgtctcgag tgctcccgat
     4681 tcggaccgcg cctggctgtc ggccatcgcc caggcctcgg gcggctcggc gcttcagctc
     4741 ggccccggta actccgagca gcttctcgac cggctcgcgg gaagcacgcc gcgcgttctg
     4801 gcggtccgga ccgccgccgg ggcgccgata gacttcacct tgctcgatgc gccggagaag
     4861 ggctggcgcc tggttggtcc gctcccgagc agccgaagcg tcgtcgttcg tctcgccggc
     4921 cccaatggcc ggcccatcga gcgagtctac gccaccggcg agggcgaggc tccccgcctc
     4981 aacggcgccg gagcactctg ggcctccgat cggcttgccc tgaaggtggc gaacgacgat
     5041 tccgaccggg acgacatcgt cgctttcgcc cggcgccaca gggtggccgc tccggacgtg
     5101 tccttcatcg tgctcgagcg gccggccgac tatgccaggg cgggtatcga gccgccgctc
     5161 gcttatgcgg cgtccaagcg cgccgagttc gaccgcctgc tggcggaaaa gctggccgat
     5221 gagcgcaggg accaggaggc caggctcgca accgtgatcg aggcctggga gagccagaag
     5281 gcatggtgga agaagcggtt cgaccccgac gccaagacga acaagggcaa ggaaaatgcc
     5341 gctcctccgc cgccgcctcc ggcaccttcg gctccgcctc aaccggtgac ggcccaacct
     5401 cctgccgaag agcccgcccc ggaccagctc aatgaattgc cgcagctgcg gtctacgaac
     5461 gacgatgctg cggagggagc ggacatcacc gtcaccggca cccggatccg gaatccgaac
     5521 ctcgactcga tggcccccgt cacgtcgatc ggcggcgaag agttcttcca gagaggcaat
     5581 gcggaggcgg agcccgccaa ggcggcgata gagcttcccg aatggacggc ggaccgtccc
     5641 tatctgaagg ccctggcggc cgctcgagct tcggagctgg agacggtgct cgcccgtgag
     5701 caggccgaac atggagcgct tcccgcattc tggctcgacg tctcggactg gtatttccgc
     5761 gccgggcgca ggcccgaggc cctgcgcctc ctgctttcgg cgctggacct gccgacccgg
     5821 aacagcgaga ccgtcgctgt ggtcgcggct cggctggtcc gctggggcga gctcgaccgg
     5881 gccgtgcagc tcttcgagcg cctggccgcg gccgaatccg atcgcccgca gccccttcgc
     5941 agcctagccc tggcccttgc gaagcgggcc gaagccggtc cctcgcccca gtcgcgagcg
     6001 gacctggccc gagccatggc cctattgacc caggtcgtga tgacgccgtg gggcgaggat
     6061 tatgacggca tggagatgat ctcgctgatg gaggtgaacc gcctgatccc gcgctaccgc
     6121 aagctcggcg gggccgacgt cccgctcgac ccgcgcctta tcgctttgct cgacgtcgac
     6181 ctgcgagtga cgatcgaatg gaatacggag gaaaccgacc tcgatctgtg ggtcgacgag
     6241 ccgaacggcg agcgcgccat gtacagcaat cccgccacgg cgatcggagg gcggctctcg
     6301 aacgacatga cgaacggcta tggcccggag gaatatttgc ttcgccgggc gcccgacggc
     6361 accttcacgg tgcgcgccga cgtc
//
LOCUS       DASWNU010000227         9021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_476854, whole genome shotgun sequence.
ACCESSION   DASWNU010000227 DASWNU010000000
VERSION     DASWNU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476854"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VGB57_04440"
     CDS             complement(<1..660)
                     /locus_tag="VGB57_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0130633.1"
                     /translation="MRAAIFLLLAGAAAVPAASAQPGGKAPATSVNWAGMVVATPEGG
                     FRMGNPAAKVKLIEYGSLTCSHCATFAKEGMESLVGSYVRSGKVSYEYRNLVLNGLDV
                     AATMVARCGGAGRFFPVADKLYATQDQWKGRVKELTDAQKQALNALPENQRLGRLAEL
                     AGIPEIAAQHGIAPASAKTCLADRAAFDALGKMNEAAAAQGVQGTPTFFLDGANLGSQ
                     TW"
     gene            complement(657..1400)
                     /locus_tag="VGB57_04445"
     CDS             complement(657..1400)
                     /locus_tag="VGB57_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0130634.1"
                     /translation="MKSMLMAGAAALALLLTGCGDGGGNQSAPVDNSALKQIPAPNNG
                     DWTQTVVKTPEGGFRMGNPEAPVKLVEYGSLSCPHCAEFEEQGGPALRDNYVKSGQVS
                     WEFRTYLLFPTDPGVSLLANCQSENAFFPAVEQLYKDQSAWIGRIQSMPQDQLQALGT
                     MPPRESAAIVARAAGLDEFFRQRGMPSSQIQSCLSDPAGLEKLADIQRKGGEMGVNGT
                     PTFFINGKLVEGAASWRALEPAIREAIGQ"
     gene            complement(1397..1963)
                     /locus_tag="VGB57_04450"
     CDS             complement(1397..1963)
                     /locus_tag="VGB57_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY0130635.1"
                     /translation="MAKSGGISKKQAEAPRANRARAVSDIVPDIGRAAFRRFGFVQSS
                     IVSRWREIVGDRYAAVSSPESIRFPAGKRSDGVLNLIVEGAHAPMMQHVAPVIVERVN
                     RFFGYPAVARVAFRQGLVQIARARTRAAPPSLRPIPVELGDSLREVADPELRACLESL
                     ARGVAANEAALGAAVAIPVLGRIGETKR"
     gene            1990..3105
                     /gene="mutY"
                     /locus_tag="VGB57_04455"
     CDS             1990..3105
                     /gene="mutY"
                     /locus_tag="VGB57_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY0130636.1"
                     /translation="MSVDASDALLRWFAVDKRRLPWRAEGAERPDPYRVWLSEIMLQQ
                     TTVAHASPYFETFTRRWPTVDRLAAAADEEVMTAWAGLGYYARARNLIACARKVAEEY
                     GGRFPDSEEGLRKLPGVGRYTAAAIAAIAFGRRAVVVDANVERVVARLFAVETALPAA
                     REEIYRLAGSITPEAESGDFAQGMMDLGATICTPRSPDCGRCPLASLCAGRATGHPHL
                     YPRKAAKAPRPRREGIAYWLEHDGHVLLVRRPARGLLGGMLALPTDSEGAPADARWRE
                     AGSVDHVFTHFALTMKLLCADARERPEGTWWPIERIGEAGLPTLFAKLASLSLKWRGG
                     GPVRRQWGGEGTVQERQALTLPPLRGGPLPLPCRERG"
     gene            3105..3971
                     /gene="nudC"
                     /locus_tag="VGB57_04460"
     CDS             3105..3971
                     /gene="nudC"
                     /locus_tag="VGB57_04460"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY0130637.1"
                     /translation="MLPPGFTGAGLDRADHLRLDEARLAELAASAEARLLRLSGLDPE
                     LDEDGRLGWGAMAEVEERIFPGFREGAPLFAPLIRVEPGQRAWSVFALLNRVGAEDAA
                     VWGMARSLIEWHNRHRFCGNCGSSTLAFRGGWGRRCGGCGLDHFPRVDPVVIMLAEHD
                     GRVLLGRQPQYPAGRYSALAGFVEPGESIEEAVARELGEEAGIEVSDVRYVASQPWPF
                     PGQLMIACTATAAGGEIILDTNELEDAIWVDRAGVKAALAGEPGAPFLAPPHFAIAYT
                     LLRHWADLASPS"
     gene            4215..4925
                     /locus_tag="VGB57_04465"
     CDS             4215..4925
                     /locus_tag="VGB57_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEY0130638.1"
                     /translation="MDNRSNTIAGWVLGAGIVALGASIVTGEVFHQERPEKMGYPIEG
                     VVLKGEGGAAEAEVPIANLLASADVGKGEGQFKKCTACHTINQGGANGTGPNLWGVLG
                     SPIGKHSPGFAYSPALAGKGGNWDWESMSQWIKSPRDFAPGTKMTFAGIGKAEDRADL
                     LVYLNAQGSNLPLPTPQAVPAAPAGDPKAAAEKADQPAAGDKGETEPVLNEQQATPNR
                     VGGEGAPSVAGRAEQEKK"
     gene            4939..5385
                     /locus_tag="VGB57_04470"
     CDS             4939..5385
                     /locus_tag="VGB57_04470"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY0130639.1"
                     /translation="MVSSKAPTPEAYLDELEPERARFVSRLRDLINANLPEGYVERMN
                     WGMISWEVPLANYPETYNGQPLVYAGLAAQKNYTALYLNCVYASAERTERLKAQWTAA
                     GKKLDMGKSCLRFKRPGDVAEDVLAETIRSIPVERFIAEYEAGRAR"
     gene            complement(5532..6413)
                     /locus_tag="VGB57_04475"
     CDS             complement(5532..6413)
                     /locus_tag="VGB57_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0130640.1"
                     /translation="MTDPIPPSRVFPAARQEAETAAQQTQSAQTEHDAYRLAFQDMDF
                     LLREDLRPVRFQLELLKPELLLDEARIGSTFVFYGSARIPEPAKADALIAAAATDQQR
                     HIAERLKAKGKYYEEARQLARLASRCDPDDKGWRHFVVCSGGGPSIMEAANRGAHDEG
                     AESVGLNIVLMHEQMPNPYVTPALSFQFHYFALRKMHFLLRARAVAVFPGGFGTFDEA
                     FELLTLVQTGKVKPLPILFFGREFWERVVDFDALVEEGVVAPDDLKLFTFVDTAEEAW
                     KHVCDHYAANDEPVACG"
     gene            complement(6424..7056)
                     /locus_tag="VGB57_04480"
     CDS             complement(6424..7056)
                     /locus_tag="VGB57_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY0130641.1"
                     /translation="MRRAILATLLLASCIPSSGRRTEPVARDRPAPARHILQCRADLA
                     REAVRFKTLPDRAFGNGCTAIGAVQLLDIGTPVANLGAMTCPLARQFARWAREAMQPA
                     ARAWLASPVVKIESFGTYSCRPVNGRPGARLSEHGRANAVDVAAFVLADGRRITVLAG
                     WNGGDEGVRRFLRAVHAAGCRRFGIVLGPDADAFHRDHLHFDMGQGPYCR"
     gene            7295..8443
                     /locus_tag="VGB57_04485"
     CDS             7295..8443
                     /locus_tag="VGB57_04485"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY0130642.1"
                     /translation="MKVTITKPEVRPARPFFSSGPCAKPPGWGPEHLALGSLGRSHRS
                     KLGKARLGHAIELTRRVLRVPATHRIGIVPASDTGAVEMALWSMLGPRPVTLLAWESF
                     GEGWVTDVVKQLKLDSTVIRADYGELPDLAAVDPASDVVFTWNGTTSGVKVPNGDWIS
                     DAREGLTIADATSAAFAQPLPWEKIDVATFSWQKALGGEGGHGMLVLSPRAVERLESY
                     SPPWPLPKIFRLTKSGKLIEGIFHGETINTPSMLAVEDYIFALEWAVGVGGLAGLVAR
                     SEANAAAVDAWVERTGWIDHLARVPETRSNTSVCLKFAEAGDPEAQSALVKTMAGLLE
                     AEGVGYDVAGYRDAPPGLRLWCGATVDTDDLEALFPWLDWAFHEARGA"
     gene            8544..>9021
                     /locus_tag="VGB57_04490"
     CDS             8544..>9021
                     /locus_tag="VGB57_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0130643.1"
                     /translation="MPKVLISDSMDPKAAEIFRDKGVEVDERPGLSKDELKAIIGQYD
                     GLAIRSATKVTEDLLGAATNLKVIGRAGIGVDNVDIPAATARGIVVMNTPFGNSITTA
                     EHAIALMFALARDLPAADSSTQAGRWEKNRFMGVELTNKTLGLIGAGNIGSIVADRA"
BASE COUNT         1469 a         3065 c         3097 g         1390 t
ORIGIN      
        1 ccaggtctgc gagccgagat tggcgccgtc gagaaagaag gtcggcgtgc cctggacgcc
       61 ctgcgccgcg gccgcttcgt tcatcttgcc gagcgcatcg aacgccgccc ggtccgccag
      121 gcaggttttc gccgaagcgg gagcgatgcc gtgctgcgcc gcgatctcgg ggattcctgc
      181 gagctccgcc agccgcccaa gccgctgatt ttccggaagc gcgttcagcg cctgcttctg
      241 agcgtcggtg agttccttga cccggccctt ccactggtcc tgggtcgcgt agagcttgtc
      301 ggctaccggg aagaaccggc ccgccccgcc gcagcgcgcg accatggtcg cggcaacgtc
      361 gaggccgttc aggacgaggt tgcgatattc gtagctgacc ttgccgctgc gaacatagct
      421 gcccaccagc gactccatcc cctccttggc gaaagtcgcg caatggctgc aggtcagcga
      481 gccatattca atgagcttca ccttggccgc gggattgccc atccggaatc cgccttcggg
      541 ggtggccacc accatgccgg cccagttgac actggttgcc ggcgccttcc cgccgggctg
      601 ggcactggcg gcgggcacgg ccgccgcgcc cgccagcaga agaaagatcg ccgccctcat
      661 tgcccgatcg cctcgcgaat cgccggctcg agcgctcgcc aactggccgc gccttcgacg
      721 agcttgccgt tgatgaagaa ggtgggcgtt ccgttgaccc ccatttcgcc gcctttgcgc
      781 tggatgtccg ccaacttctc gagccccgca ggatcgctga ggcaggactg tatctgcgac
      841 gacggcatgc cgcgctgacg gaagaattca tccagcccgg cggcgcgggc gacaatggcc
      901 gcggattcgc gcggcggcat ggtcccgagc gcctggagct gatcctgagg catcgactgg
      961 atccgtccaa tccaggcgct ctgatccttg tagagctgct cgacggcggg gaagaaggca
     1021 ttctcgctct ggcaattggc gagcagggaa acacccggat cggtcgggaa gagcagatag
     1081 gtgcggaatt cccagctgac ctggccgctc ttcacataat tgtcgcgaag cgcgggcccg
     1141 ccctgctcct cgaactcggc gcaatggggg cagctcagcg agccatattc gaccagcttc
     1201 accggggcct ccggattgcc catccggaag ccgccctcgg gcgtcttgac gaccgtctga
     1261 gtccagtcgc cgttgttcgg cgccgggatc tgcttcagag cgctgttgtc gaccggagcc
     1321 gattgattgc cgccgccgtc gccgcagccg gtcagtaaaa gcgccagagc ggccgcaccg
     1381 gccatgagca tcgacttcat cttttcgtct ctccaattct gccgagcacc gggatggcga
     1441 ccgccgcccc cagcgccgcc tcgttagccg cgactccgcg ggccagcgac tccaaacagg
     1501 cgcgaagctc cggatccgcc acctcgcgca aggaatcccc gagctcgacc gggatcgggc
     1561 gaagggacgg cggcgcggcc cgggtcctgg cccgtgcaat ctgaaccagg ccttgcctga
     1621 aggcgaccct ggccaccgcc ggatagccga agaagcggtt gacccgctcg acgatcaccg
     1681 gagcgacatg ctgcatcatc ggagcgtgag cgccctcgac gatcaggttc aagactccat
     1741 ccgaacgctt gccggcaggg aaacggatcg attcgggcga ggagacggcg gcgtagcggt
     1801 cgccgacgat ctcgcgccag cggctgacga tcgacgactg gacgaagccg aagcggcgga
     1861 aggcggcccg gccgatatcg gggacgatgt ccgagaccgc ccgggcgcga ttcgcccgcg
     1921 gcgcctccgc ctgctttttc gaaatgccgc ccgatttcgc catccggccg atcatggcat
     1981 aggcacagga tgtcagtcga tgcttccgat gccctcttgc ggtggtttgc tgtggataaa
     2041 cggcgactgc cctggcgggc ggaaggcgcg gagcggccgg atccctatcg cgtgtggctg
     2101 tccgagatca tgctccagca gaccaccgtc gcccatgcct cgccctattt cgagaccttc
     2161 acccgccgct ggcccaccgt cgacaggctg gccgcggccg cggacgagga ggtcatgacg
     2221 gcctgggcgg ggctcggcta ttacgcccgg gcccgcaacc tgatcgcctg cgcgcgcaag
     2281 gtcgccgagg aatatggcgg acgcttcccc gacagcgagg aagggctgcg caagcttccc
     2341 ggcgtcggcc gctataccgc cgccgcgatc gcggcgatcg ccttcgggcg gcgggcggtg
     2401 gtggtcgatg ccaatgtcga gcgggtcgtc gcccggctgt tcgcggtcga aaccgcgctt
     2461 ccggcggcgc gggaggagat ttaccgcctc gccggctcga tcacgccgga ggccgaatcc
     2521 ggcgacttcg cccaggggat gatggacctc ggcgccacga tctgcacccc gcgctcgccc
     2581 gattgcggcc gctgcccgct tgcctcgctc tgcgctggcc gggcgaccgg gcatccccat
     2641 ctctatcccc gcaaggccgc gaaggcgccc aggccgaggc gggaggggat cgcctattgg
     2701 ctcgagcatg acggccacgt cctcctcgtc cggaggcccg ccaggggcct gctcggcgga
     2761 atgctggcgc tcccgaccga cagcgagggc gctccggccg acgcccgctg gcgggaggca
     2821 gggagcgtcg accatgtctt cacccatttc gcactcacca tgaagctgct ctgcgccgac
     2881 gcccgggagc ggccggaggg gacctggtgg ccgatcgagc ggatcggcga ggccggcctt
     2941 cccaccctgt tcgccaaact cgcgtccctc tccttgaagt ggagaggggg gggcccagtg
     3001 cgccggcaat gggggggtga gggcacggtt caggagaggc aggccctcac ccttccaccg
     3061 cttcgcggcg gccccctccc tctcccgtgc agggagaggg gttgatgctg ccccccggct
     3121 tcaccggcgc cggcctcgac cgagccgacc atctccggct cgacgaggcg cggctggccg
     3181 agcttgcagc gagcgccgag gcgcggctgc ttcgcctcag cggcctcgac ccggagctgg
     3241 acgaggacgg ccggctcggc tggggcgcga tggcagaggt ggaggagcgc atcttccctg
     3301 gcttccgcga aggcgctccg ctgttcgcgc ctctgatccg ggtcgaaccg gggcagcgcg
     3361 cctggtcggt cttcgccctg ctcaaccggg tgggggctga ggatgccgcc gtatggggca
     3421 tggcccgaag cctgatcgaa tggcataacc gccaccgttt ctgcggcaat tgcggctcct
     3481 cgacgctcgc gttccggggc ggctggggcc ggcgctgcgg cggctgcggg ctcgatcatt
     3541 tcccgcgcgt cgacccggtc gtgatcatgc tcgccgagca tgacgggcgg gtattgctcg
     3601 gccgccagcc gcaatatccg gccgggcgct attccgccct cgccggcttc gtcgagcccg
     3661 gcgaatcgat cgaggaagcg gtggcccgcg agctcggcga ggaagcggga atcgaggtga
     3721 gcgacgtccg ctacgtcgcc agccagccct ggcccttccc aggccagctg atgatcgcct
     3781 gcaccgcaac cgcggccggc ggcgagatca ttctcgacac gaacgagctg gaggacgcga
     3841 tctgggtcga ccgcgcgggc gtgaaggcgg cgcttgcggg ggagccgggg gcgcccttcc
     3901 tcgcaccgcc gcacttcgcg atcgcttata cgctgctgag gcactgggcc gatcttgcct
     3961 ccccatcgtg agatggggag ggggaccatg cgaagcatgg tggaggggta gaaggcgaag
     4021 gattcgggtg gctcgcgcgc gaaccgttca gtcggcgcca attacccctc caccacactt
     4081 cgtgtggtcc ccctccccat gcttcgcacg gggaggcaag ccgtgtcttg ccccgtctcc
     4141 ctcccccgcc taaaggggct cgcgaattcc ctcatcccgc gcgtccgcgg gccgccctgg
     4201 aggtctttga aggcatggac aaccgctcca acacgattgc gggctgggtg ctcggcgccg
     4261 gcatcgtcgc gctcggcgcc tcgatcgtca ccggcgaggt gttccaccag gagcggcccg
     4321 agaagatggg ctatccgatc gagggcgtgg tcctgaaggg cgagggcggc gccgccgagg
     4381 ccgaagtgcc gatcgccaat ctcctcgctt ccgccgacgt cggcaagggc gagggtcagt
     4441 tcaagaaatg caccgcctgc catacgatca accagggcgg cgccaacggc accggcccca
     4501 atctttgggg cgtgctgggc agcccgatcg gcaagcactc gccgggcttc gcttattccc
     4561 cggcgctcgc cggcaagggc ggaaactggg attgggagtc catgagccag tggatcaagt
     4621 cgccgcgcga cttcgctccc gggaccaaga tgaccttcgc cggaattggc aaggccgagg
     4681 accgggccga tctcctcgtt tatctcaatg cgcagggcag caacctgccg ctgccgacgc
     4741 ctcaggccgt ccccgccgcc ccggcgggag atcccaaggc cgccgccgag aaggccgacc
     4801 agcccgccgc gggcgacaag ggggagacgg agcccgtgct gaacgagcag caggcgactc
     4861 cgaaccgggt cggcggcgag ggcgcgccct cggtcgcggg ccgggcggag caggaaaaga
     4921 aatagcaggc caacggggat ggtcagcagc aaggcgccca cccccgaagc ctatctcgac
     4981 gagcttgagc cggagcgggc ccggttcgtt tcgcgccttc gcgacctgat caacgccaat
     5041 cttcccgaag gctatgtcga gcggatgaac tggggaatga tcagctggga agtcccgctg
     5101 gccaattatc ccgaaaccta taacggccag ccgctcgtct acgcgggcct ggcggcgcag
     5161 aagaactaca ccgccctcta cctcaactgc gtctatgcct cggccgagcg gaccgagcgg
     5221 ctgaaggcgc agtggacggc cgcgggaaag aagctcgaca tgggcaagag ctgcctccgc
     5281 ttcaaacggc ccggggacgt cgccgaggac gtgctggccg agacgatccg ctcgatcccg
     5341 gtggagcgat tcatcgccga atacgaggct ggccgggcga gataggatta ccttgcctcc
     5401 ccgcgccgaa ggcgggggag ggggaccctg cgcagcatgg tgcaagggta atggacgagg
     5461 cttgctccag ttacccctgc accacccttc gggtgatccc cctccccatg cattcgcatg
     5521 gggaggcaag atcagccgca ggccaccggc tcgtcattgg ctgcgtaatg gtcgcagaca
     5581 tgcttccacg cttcctccgc cgtgtcgacg aaggtgaaca gcttgagatc gtcgggcgcg
     5641 accacgcctt cctcgaccag ggcgtcgaaa tcgaccaccc gctcccagaa ttcgcggccg
     5701 aagaagagga tcggaagcgg cttgaccttg ccggtctgga cgagggtgag aagctcgaac
     5761 gcctcgtcga acgtaccgaa gccgccgggg aagacggcga cggcgcgtgc tcggagcagg
     5821 aagtgcatct tcctcagggc gaaatagtgg aactggaagc tcagggccgg agtgacatag
     5881 gggttgggca tctgctcgtg catcaggacg atgttgagcc cgaccgattc ggccccttcg
     5941 tcatgagcgc cgcggttcgc cgcctccatg atcgagggcc cgccgcccga acagacgacg
     6001 aaatgcctcc agcccttgtc gtccggatcg cagcggctgg cgaggcgggc aagctgccga
     6061 gcttcctcat aatatttgcc cttggccttg agccgctcgg cgatgtggcg ctgctggtcg
     6121 gtggcggcag cggcgatcag ggcgtccgcc ttggccggct cgggaatgcg cgccgagccg
     6181 tagaagacga aggtcgagcc gatcctggcc tcgtcgagca gcagctcggg cttcagcaat
     6241 tcgagctgga agcggaccgg gcgaagatcc tcgcgcagga ggaagtccat gtcctggaag
     6301 gcgaggcgat aggcatcgtg ctcggtctgc gcggactgag tctgctgggc ggcggtctcc
     6361 gcttcctggc gggccgccgg gaagacgcgg ctgggcggta tgggatcggt catggcggcc
     6421 cgtttagcgg caatacggcc cctgtcccat gtcgaaatga agatggtcgc gatggaaggc
     6481 gtcggcgtcg gggccgagca cgattccgaa gcgccggcag ccggcggcat gaacggcgcg
     6541 aaggaagcgg cgaacgccct cgtcgccacc gttccagccg gcgaggacgg tgattcggcg
     6601 accgtcggcg aggacgaagg cggcgacgtc caccgcattg gctcggccat gctcgctgag
     6661 ccgggcgccg ggccggccgt tgaccggccg gcagctatag gtgccgaagc tctcgatctt
     6721 gacgaccggg cttgcgagcc aggccctggc cgcgggctgc atcgcctcgc gcgcccagcg
     6781 cgcgaactgg cgcgccagcg ggcaggtcat cgctcccaga ttggcgacgg gcgtgccgat
     6841 gtcgaggagc tggacggcgc cgatcgcggt gcagccgttt ccgaaggcac ggtcggggag
     6901 ggtcttgaag cggacggctt cgcgggcgag atcggcgcgg cactggagga tatgccgggc
     6961 cggagcgggg cggtcgcggg cgacgggttc ggtccggcga ccgctggagg ggatgcagct
     7021 tgcaagaagc aaggtcgcca ggatcgcccg tctcatgctc gaagctctac gccgcgagca
     7081 ccgctccctc atcccgggag ggaccccatc gaagtactac ttcgatggga acccgaaagc
     7141 cgggacctca ggacgagaag gcacggcggg tctccccgag gcccccgctt tcgccggggt
     7201 gacgggcgcg agcggttgac aggacgtggc gcgcccctaa accccgccac gggccacgcc
     7261 gtcccaacgc ggcgcgtgct ctctgcgagg agagttgaaa gtgacgatta cgaaacccga
     7321 ggtgcgcccc gcgcgtccct tcttctcttc cggaccgtgc gccaagccgc ccggctgggg
     7381 ccccgaacat ctcgctctgg gctcgctcgg gcgatcgcac cgctcgaagc tcggcaaggc
     7441 gcggctcggc cacgcgatcg aactgacccg gcgggtgctc cgggtgccgg ccactcaccg
     7501 catcggaatc gtcccggcct ccgacaccgg cgccgtcgaa atggccttgt ggtcgatgct
     7561 cggcccccgg ccggtgaccc tgctcgcctg ggagagcttc ggcgaggggt gggtgaccga
     7621 cgtcgtcaag cagctcaagc tcgactcgac ggtgatcagg gcggattatg gcgagcttcc
     7681 cgacctcgcc gccgtcgatc cagcgagcga cgtcgtcttc acctggaacg gcaccacctc
     7741 gggagtcaaa gttccgaacg gcgactggat cagcgacgcc cgcgaaggcc tgaccatcgc
     7801 cgacgccacc tccgccgcct tcgcccaacc cctgccctgg gagaagatcg acgtcgccac
     7861 cttcagctgg cagaaggcgc tgggcggcga gggcggccac ggtatgctcg tcctcagccc
     7921 gcgcgcggtc gagcggctcg aaagctattc gccgccctgg cctctgccca agatcttccg
     7981 gctcaccaaa agcggcaagc tgatcgaggg catcttccac ggcgagacga tcaacacgcc
     8041 gtcgatgctg gcggtcgagg attatatctt cgcgctggaa tgggcggtcg gggtcggcgg
     8101 ccttgccggc ctcgtcgccc gctccgaagc caatgccgct gcggtggatg cctgggtcga
     8161 gcgcaccggc tggatcgatc atctcgcccg tgtgccggag actcgctcca acaccagcgt
     8221 ctgcctcaag ttcgccgagg ccggcgaccc ggaagcgcag tcggccctgg tcaagacgat
     8281 ggccggcctg ctcgaggcgg aaggggtcgg ctacgacgtc gccggctatc gcgacgctcc
     8341 gccgggcctc cggctatggt gcggggcgac ggtcgatacc gacgatctcg aggcgctctt
     8401 cccctggctc gactgggcct ttcacgaggc gaggggcgcc tagccccgtc atcccggcgg
     8461 aagccggaac ctcgggacgg aaaagacacc gcgcccttca ccccacgagg tcccggcttt
     8521 cgccgggatg acggagtaac gaaatgccca aagtgctgat ttccgattcc atggacccca
     8581 aggcggcgga aatcttccgc gacaagggcg tcgaggtcga cgaaaggccc ggcctttcca
     8641 aggacgagct gaaggcgatc atcggtcagt atgacggcct cgccatccgc tccgcaacaa
     8701 aggtcaccga ggatttgctc ggagcggcga cgaacctgaa ggtgatcggc cgggccggaa
     8761 tcggagtcga caatgtcgac atccccgccg ccacggcccg cgggatcgtc gtgatgaaca
     8821 cgcccttcgg caattcgatc accaccgcgg aacatgccat cgccctgatg ttcgccctcg
     8881 cccgcgacct tcccgccgcc gattcctcaa cccaggcggg caggtgggag aagaaccgat
     8941 tcatgggggt ggagctgacc aacaagacgc tcgggctgat cggggccggc aatatcggct
     9001 cgatcgtcgc cgaccgggcg c
//
LOCUS       DASWNU010000228         4427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_371356, whole genome shotgun sequence.
ACCESSION   DASWNU010000228 DASWNU010000000
VERSION     DASWNU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371356"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="VGB57_04495"
     CDS             <1..2379
                     /locus_tag="VGB57_04495"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEY0130644.1"
                     /translation="STLLSGLGGRLGLDGDARMTRGALDLASAAARIGGITASRLTVQ
                     GRYGLSLDNGGFTLLGDASARGASARGPLAGAVAALGSAGGTPLEPVGDALAAALSRA
                     SGAFDADASLRLVNGRGFGGIRFERLGAATRSGARVELGGGQGLTYYWPGGLSRLDGD
                     LAVSGGGFPAMRLSLSQPRPGAPVSGTGRIQPIRVGNSRLALGDISFTAAAGGETRIA
                     TVATIDGPLDNGWVRGLVLPIAGRLDGRGGFAFGESCTPARFEALQVGTLRLGRTLLP
                     LCPTGRALVWKTPGGPVAGGAELRSPRFAGTLGASPFSVAGSRLRFGLAGPGFAASDL
                     EVRLAANRFDLGSLTGDFGEGGVTGRFAGLSGKLANVPLLMSQGNGTWSMRGADLAMA
                     GEMRVSDEVDPPRFYPLVTRDFRLTLVDGQIKAGGWLDDPETGTRIVRADIGHSLATG
                     RGRTALDVPGIRFDKNYQPEQLTRLTTGVVALVNGILKGQGEISWDGTGSRSSGTFST
                     DDMDFAAAFGPVEGLSTSLHFTDLLGLVSAPGQEAQIGLVRAGIDVLDGRLRYRLLPG
                     MQVKVESGRWPFAGGELILEETVLDFSKPSAKRLTFRVEGLDAALFIQKMEFANITAT
                     GTFDGVIPMVFDERGGRIVGGHLIARPEGGTLSYIGELTDKELGVYGKLAFDALKSMR
                     YSKLTIDLDGSLEGEFVAGIELDGVARNTALTVAPSGGIKGMVASRALTQLAKIPFEF
                     NITVKGPFRALIGTMRSLHDPTLLIQSTLPDLIRGKPDEPPAVQPQESEIVR"
     gene            2427..2609
                     /locus_tag="VGB57_04500"
     CDS             2427..2609
                     /locus_tag="VGB57_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEY0130645.1"
                     /translation="MRRMKILLAAPVVLVSAGCVQVSAPEKPIEINLNINIRQEVVVR
                     LQQDVRDLIKENPGVF"
     gene            2623..3000
                     /locus_tag="VGB57_04505"
     CDS             2623..3000
                     /locus_tag="VGB57_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEY0130646.1"
                     /translation="MKLILAAGIGLGAVLGTTAAHAMLQDASALLRASGQVGEQADGY
                     LGLVGSAPAAVRAQVDSVNIKRRAYYTDLAAKRGAKIEEVAATTACELFRTKVAQGQY
                     YRLPDGVWRQRDSAPIPLPSYCG"
     gene            3304..3621
                     /locus_tag="VGB57_04510"
     CDS             3304..3621
                     /locus_tag="VGB57_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY0130647.1"
                     /translation="MAEDEPRQDPKLPPDARLDSLEERLKVAHAAEAARTGVKQRPPQ
                     TGHRQGMRILSVMFSYPLGSALIGWVIDRAAGTRGIWVAMLFLGFGAAMWEVWKISKQ
                     KPE"
     gene            3642..4412
                     /locus_tag="VGB57_04515"
     CDS             3642..4412
                     /locus_tag="VGB57_04515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0130648.1"
                     /translation="MAAESKIDPMHQFQISPLAGNLDGNPFVFTNSALWMLVVLALIW
                     IFMLGGMKRQLVPGRWQVMVEGVTGFIHSMVTTNIGPEGRKYMPWVFTVFMFILFANL
                     LGLMPFALVGAQPFTVTSQFTVTGIMAIISFAIVLIVGFWRHGFHFFSLFIPHGTPKV
                     MLPMIFLIELLSFMVRPFSLALRLFVAMIAGHILVEVFGSFVVQGINAGGVGPAISLL
                     SFVFIVGVMALEILVCAIQAYVFALLTSLYINDAVNLH"
BASE COUNT          662 a         1588 c         1405 g          772 t
ORIGIN      
        1 tcgacgctgc tgagcggcct tggcgggcgc ctcggcctcg acggcgatgc ccggatgacc
       61 cgaggcgccc tcgacctcgc ctccgccgcc gcccggatcg ggggaatcac cgcgtctcgc
      121 ctgaccgttc agggccgcta tggactgtcg ctggacaatg gcggcttcac cctgctcggc
      181 gatgcctcgg cccggggcgc atccgcacgc gggccgctgg cgggggccgt cgcggccctg
      241 ggctcggcgg gaggcactcc gctggagccg gtgggcgacg cgctcgccgc cgccctctcc
      301 cgagcctccg gcgccttcga cgccgacgcc tccctccggc tcgtcaacgg ccgaggcttt
      361 ggcggcatcc gcttcgagcg gctgggcgcg gccacccgaa gcggcgcccg ggtcgagctc
      421 gggggagggc aggggctcac ttattactgg cccggcggcc tcagccggct ggacggcgac
      481 ctagccgtct ccggcggcgg ctttccggcc atgcggctca gcctttcgca gccgcgcccg
      541 ggggcaccgg taagcggcac cggccggatc cagccgattc gggtcgggaa ttcgcggctt
      601 gcgctcggcg acataagctt caccgccgcg gccgggggtg agacccggat cgccacggtg
      661 gcgacgatcg acggcccgct cgacaacggc tgggtgcgcg ggctggtgct cccgatcgcc
      721 ggccgcctcg acggccgagg cggcttcgct ttcggggaaa gctgcacgcc tgcccggttc
      781 gaggcgctcc aggtcggaac gcttcgcctc ggccgcacct tgcttccgct ctgcccgacc
      841 ggacgcgctc tggtctggaa gactcccggc ggcccggtcg ccggcggcgc cgagcttcgc
      901 tcgccccgct tcgccggaac cctcggcgcc tcgccgttca gcgtcgccgg aagtcgcctc
      961 cgcttcggcc tcgccggccc gggcttcgcc gcctccgacc tcgaggtccg cctcgcggcc
     1021 aaccgcttcg acctcggctc gctgaccggc gattttggcg aaggcggagt caccgggcgc
     1081 ttcgccggcc tctccggcaa gctcgccaac gtgcccctgc tgatgagcca ggggaacggc
     1141 acctggagca tgaggggcgc cgatctcgcc atggccggcg agatgcgggt ctcggacgag
     1201 gtcgatccgc ctcgcttcta cccactcgtc acccgcgact tccgcctgac cctcgtcgac
     1261 ggccagataa aggccggtgg ctggctcgac gatccggaga cggggacacg catcgtcaga
     1321 gccgacatcg gccacagcct ggccaccggc cggggccgga ccgcactcga cgtccccgga
     1381 atccgcttcg acaaaaatta ccagcccgag cagctcaccc gcctcaccac cggtgtcgtc
     1441 gctctcgtca acggcatcct caaggggcaa ggcgagatca gctgggacgg gaccggctcg
     1501 cgctcgagcg gcaccttctc gaccgacgac atggatttcg ccgccgcctt cgggccggtc
     1561 gagggcctat cgaccagcct tcatttcacc gacctgctcg gcctcgtcag cgctccgggc
     1621 caggaggcgc agatcggcct cgtccgggcc gggatcgacg tgctcgacgg ccgcctccgc
     1681 tatcgcttgc ttcccggaat gcaggtcaaa gtggaaagcg gccgctggcc cttcgcgggt
     1741 ggcgagctga tcctcgagga gaccgtcctc gatttctcca agccctcggc caagcgtctc
     1801 accttccggg tcgagggcct cgacgccgcg ctcttcatcc agaagatgga attcgccaac
     1861 atcaccgcca ccggaacctt cgacggagtg atcccgatgg tcttcgacga gcgcggcggc
     1921 cggatcgtcg gcggtcatct gatcgcccgt ccggaaggcg gcacgctgtc ctatatcggc
     1981 gagctcaccg acaaggagct cggcgtctac ggcaagctcg ccttcgacgc cctcaaatcg
     2041 atgcgctatt cgaagctgac catcgacctc gacggctcgc tcgagggcga gttcgtcgcc
     2101 gggatcgagc tcgacggggt cgcccgcaac acggcgctta cggtcgcgcc ttcgggtggc
     2161 atcaaaggca tggtcgcgag ccgcgccctg acccagctcg ccaagattcc gttcgagttc
     2221 aacatcaccg tcaagggccc cttccgcgcc ctgatcggga cgatgcgctc gctccacgac
     2281 cccactttgc tcatccagtc cacgctcccg gacctgatcc gcggcaagcc ggatgagcct
     2341 cccgccgttc agccccagga aagcgagatt gtgcgatgaa gcggtcgatg ttgacgttac
     2401 tgcgtggcga tgcgacgcag ggcgcgatga gacggatgaa gatcctcctc gccgcgccgg
     2461 ttgttctagt ctccgccggg tgcgttcagg tgagcgctcc ggagaagccg atcgagatca
     2521 acctgaacat caacatcagg caggaggtcg tcgtccgtct ccaacaggac gtgcgcgatc
     2581 tcatcaaaga gaatccggga gtgttttgaa atgagccgcc gaatgaaact gatcctcgcc
     2641 gcgggcatcg ggcttggcgc cgttctgggc acgaccgccg ctcacgccat gcttcaggac
     2701 gcctccgctt tgcttcgcgc ctcgggccag gtcggcgagc aggccgacgg ctatctcggc
     2761 ctggtcggct cggctccggc cgcagtccgc gcccaggtcg attctgtcaa catcaagcgc
     2821 cgcgcctatt acaccgatct cgccgccaag cgcggcgcca agatcgagga agtggcagcc
     2881 accaccgcct gcgagctctt ccgcaccaag gtcgcccagg gccaatatta ccgcctcccc
     2941 gacggagtct ggcgccagcg cgactccgct ccgatcccgc ttccgagcta ttgcggctaa
     3001 gcttgtcctc cccgcgcgaa gcggggggtg ggggaccttg cgaagcttgg tggaggggta
     3061 gctggcgcga tactggtggg ttcccaagcg atccgctcaa gcctgcgccc tctacccctc
     3121 caccaccctt cgggtggtcc ccctccccat ctccgatggg aggattactg caccgcacca
     3181 tcgatgttga cttgaccggc cccccttcct aaaggggcgg cgcctcggcg gggttcgttc
     3241 gccggcatgt cctcgcaccc tcatcagccc gctcccgagc gccgctgtcc atggaagaac
     3301 gacatggcgg aagacgagcc acggcaggac ccgaagcttc ccccggacgc gcggctcgac
     3361 tcgctcgaag agcggctcaa ggtagcgcat gcggcggaag cggcgaggac cggcgtgaag
     3421 cagcgcccgc cgcagaccgg ccatcgccag gggatgcgca tcctctcggt gatgttcagc
     3481 tacccgctcg gaagcgccct gattggctgg gtgatcgacc gggcggccgg gacccggggt
     3541 atatgggtgg cgatgctgtt cctcgggttc ggcgccgcca tgtgggaagt ttggaagatt
     3601 tcgaagcaaa agcccgaata ggggctcgag ggacgtaaag agtggcggcc gaaagcaaga
     3661 tcgacccgat gcaccagttc cagatcagcc cgctcgcggg caatctggac gggaacccgt
     3721 tcgtcttcac caacagcgcg ctctggatgc tcgtcgtcct cgcgctgatc tggatcttca
     3781 tgctcggcgg gatgaagcgt cagctcgttc ccggccgctg gcaggtcatg gtcgagggcg
     3841 tcaccggctt catccactcg atggtgacga cgaacatcgg ccccgagggg cgcaagtaca
     3901 tgccgtgggt gttcacggtg ttcatgttca tcctgttcgc caacctcctc ggactgatgc
     3961 ccttcgccct cgtcggcgcc cagcccttca ccgtcaccag ccagttcacc gtcaccggga
     4021 tcatggcgat catcagcttc gcgatcgtcc tgatcgtcgg cttctggagg cacggcttcc
     4081 acttcttctc tttgttcatc ccccacggca cgcccaaggt gatgctcccg atgatcttcc
     4141 tcatcgagct tctctccttc atggtccgcc cgttcagcct cgcgctccgg cttttcgtcg
     4201 cgatgatcgc cggccacatc ctcgtcgagg tgttcggaag cttcgtcgtc cagggcatca
     4261 acgccggcgg ggtcggcccg gcgatcagcc tgctgtcctt cgtcttcatc gtcggggtga
     4321 tggcgctcga gatcctcgtc tgcgcgatcc aggcctatgt cttcgccctt ctgacgtcgc
     4381 tctacatcaa cgacgcggtc aaccttcact agcttcaacc gattttc
//
LOCUS       DASWNU010000229         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_86390, whole genome shotgun sequence.
ACCESSION   DASWNU010000229 DASWNU010000000
VERSION     DASWNU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86390"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..>1717)
                     /locus_tag="VGB57_04520"
     CDS             complement(199..>1717)
                     /locus_tag="VGB57_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0130649.1"
                     /translation="VTLTADGRVSYDAGSIFAGLEAGQTAIDSFTYAIVDEFGLSSTA
                     TATVTITGITHNRAPVAGNDALTIAEDAGASDVTATLLANDSDPDGDQFQMASVQGIS
                     AQGAIVTLLPDGKVSYDPGAIFAGLEAGQTATDSFTYTITDAHGLSSTATATVTIAGV
                     SSNRAPVAANDRLTIPEDAGEIDLTATLLINDTDADGDALIISAVQAVSAKGATVTLL
                     ADGHVRYDSGQLFYSLEDGDWMTDSFTYTVTDAAGATATATATLRIEGVTQITDAYFQ
                     VSEDGTTFDMLGSLIESFSFDFAGIEADGLLGTLNWDEVNGMLTFTADHASSDRLSPD
                     SWQWTYFNVLGTEGENFLIGMQIFGVNDGVVAVDDSVSVAEGGSSGNLWATLMANDTD
                     VDSSPIGRRILSVDTTGTQGSVLFDAATKTLTYSAAGIDLAPGETITDTFTYTVSDSV
                     SGSQPDTATVTVTVTGGAAMSAFLAEGEAEVFGADFGGYALMQAEQLMIADMPIA"
BASE COUNT          263 a          568 c          601 g          285 t
ORIGIN      
        1 cgcccgacag caaagctccc cacagaaaga tcggcgccag cgtcagctgg aacgggaggc
       61 ggagatgcgc gaaatagggc gcgggacggc ggatcgagtc catggcccgc gctgctcgca
      121 cgccgcgcgg ccgccgcgca agcgaaaggc cgcccccgtc gccgggagcg gccttcctgc
      181 gtccgtcgcc gcgtccggtc aggcgatcgg catatcggcg atcatcagct gctccgcctg
      241 catcagagcg tagccgccga agtccgcgcc gaagacctca gcctcgccct cggcgaggaa
      301 ggcggacatc gcggcgccgc cggtcacggt caccgtcacc gtcgccgtgt ccggctggct
      361 cccggagacg ctgtcggaca ctgtgtaggt gaaagtgtcg gtgatcgtct cgcccggcgc
      421 gagatcgatg ccggcggcgg agtaggtcag cgtcttggtc gcggcgtcga acaggacgct
      481 gccctgggtg ccggtcgtgt cgaccgacag gatgcggcgg ccgatcgggc tcgaatcgac
      541 gtcggtgtcg ttcgccatca gcgtcgccca gaggttgccg gagctccccc cttcggcgac
      601 cgagaccgaa tcgtcgacgg cgaccacgcc gtcattgacg ccgaagatct gcatgccgat
      661 caggaagttc tcgccttccg tgccgaggac gttgaaatag gtccactgcc agctgtccgg
      721 gctgaggcgg tcgctcgagg catggtcggc ggtgaaggtc agcatgccgt tcacctcatc
      781 ccagttcagc gtgccgagca ggccgtccgc ctcgattccg gcgaaatcga agctgaagga
      841 ttcgatcagc gagccgagca tgtcgaaggt cgtcccgtcc tcggagacct ggaaataggc
      901 gtccgttatc tgggtgacgc cctcgatccg cagcgtcgcc gtcgccgtcg ccgtcgcccc
      961 ggcagcgtcg gtcaccgtgt aggtgaagct gtcggtcatc cagtcgccgt cctcgagcga
     1021 gtagaagagc tggcccgaat cgtagcggac gtggccgtcc gccaggagcg tcacggttgc
     1081 gcccttggcc gacaccgcct gcaccgccga gatgatcagc gcatcgccat cggcgtccgt
     1141 gtcgttgatg agcaaagtcg ccgtcaggtc gatctcgcct gcatcttcgg ggatcgtcag
     1201 ccggtcgttg gcggccaccg gcgccctgtt cgacgagacg ccggcgatcg tcaccgtcgc
     1261 cgtcgcggtc gagctgaggc catgcgcgtc ggtgatcgtg taggtgaagc tgtcggtcgc
     1321 ggtctggccg gcttcgaggc cggcgaaaat cgcgcccgga tcgtagctca ccttgccgtc
     1381 gggcaggagc gtcacgatcg cgccctgggc ggagatgccc tgcaccgagg ccatctggaa
     1441 ctggtcgccg tccggatcgg aatcattggc gagcaaagtc gcggtgacgt cgctcgcgcc
     1501 ggcatcctcg gcgatcgtga gcgcgtcgtt ccccgccacc ggggcccggt tgtgggtgat
     1561 gccggtgatc gtcacggtgg cggtcgcggt cgagctcagg ccgaactcgt cgacgatcgc
     1621 gtaggtaaag ctgtcgatcg cggtctggcc ggcttcgagg ccggcgaaga tcgagccggc
     1681 atcgtagctg acccggccgt cggcggtcag cgtgacc
//
LOCUS       DASWNU010000230         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_163375, whole genome shotgun sequence.
ACCESSION   DASWNU010000230 DASWNU010000000
VERSION     DASWNU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163375"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VGB57_04525"
     CDS             <1..967
                     /locus_tag="VGB57_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0130650.1"
                     /translation="SWMHTPTGWTTNAAGQFVPAGSWFDIIFNPSFPYRLVHTVIAAY
                     LTTALVVGGVGAWHLLRGRDDPAVRTSFSMAMWMAALVAPVQIFVGDLHGLATLEHQP
                     AKIMAIEGYYRSYPEGAPWIAIGLPDQRAATVRYAISFPGMGSLGLHHDRHAPMAGLD
                     TIPREDWPPAPIIFWSFRIMVALGFLMFALGLWSLLARWRGRLYDWRWLHRFALLMGP
                     AGFVAVIAGWVTTEVGRQPWVIYGLLRTRGAVSPIAAPAVSASLIAFVIVYFAVFAMG
                     TWYILRLMREPPAAGEGEPARTPIRTAGITPAAAVRGRGDTGEEE"
     gene            1144..>1727
                     /gene="cydB"
                     /locus_tag="VGB57_04530"
     CDS             1144..>1727
                     /gene="cydB"
                     /locus_tag="VGB57_04530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0130651.1"
                     /translation="MDLTVVWAFIIAFAVAAYVVMDGFDLGIGILFPLFRVGKERDQA
                     MNAVAPVWDGNETWLVMGGGGLLAAFPLAYAIVLPALYAPLIAMLLGLVFRGVAFEFR
                     WRDPGHRPLWDAGFFLGSLVAAFAQGITLGALLQGISVEGRAYAGGWWEWLSPFSLLT
                     GGALVIGYALLGAAWLIWKTQGGIQQDARRAATWL"
BASE COUNT          215 a          588 c          594 g          330 t
ORIGIN      
        1 cagctggatg cacacaccca ccggctggac gaccaacgcg gccggccagt tcgtgcctgc
       61 ggggagctgg ttcgacatca tcttcaatcc gagcttcccc taccggctcg tccataccgt
      121 catagccgcc tacctcacca ccgcgctcgt cgtcggcggc gtcggcgcct ggcatttgct
      181 gcgcggccgc gacgatccgg cggtgcggac cagcttttcg atggcgatgt ggatggcggc
      241 cctggtcgcg ccggtgcaga tcttcgtcgg cgatctgcac ggcctcgcca cgctcgagca
      301 tcagccggcg aagatcatgg cgatcgaggg ctattatcgc agctacccgg aaggcgcgcc
      361 gtggatcgca atcggcctgc ccgaccagcg ggcggcgacg gtgcgttacg ccatctcctt
      421 tcccggcatg ggctcgctcg gcctccacca cgaccggcac gcgccgatgg ccggtctcga
      481 caccatcccg cgcgaggact ggccgcccgc gccgatcatc ttctggtcgt tccggatcat
      541 ggtggcattg ggcttcctga tgttcgcgct cggcctctgg agcctcctcg cccgctggcg
      601 ggggcggctc tacgactggc gctggctgca tcgcttcgct ttgctgatgg gcccggcggg
      661 cttcgtcgcg gtgatcgccg gctgggtgac gaccgaggtc ggccgtcagc cctgggtgat
      721 ctatggcttg ctgcgcaccc gcggcgccgt ctcgccgatc gccgccccgg ccgtctcggc
      781 atcgctgatc gccttcgtca tcgtctattt cgcggtgttc gcgatgggga cctggtatat
      841 tctgaggctg atgcgcgagc ctccggccgc cggcgagggc gagccggcac ggacgccgat
      901 ccggacggcg gggatcacgc cggcggcagc cgtgcgcggg cggggcgata cgggagagga
      961 agaatgatgt cgcgccacgc ccctctccct ttacgggaga gggaggggcc caagccgaag
     1021 gcttgggagg gtgagggaat gaagtatgag aactcgttca cagagtttcc tttcgcttcg
     1081 ctccctcacc ctcccaccgc ttcgcggcgg gcccctccct ctcccgaaat gggagagggg
     1141 tagatggacc tcaccgtcgt ctgggcgttc atcatcgcgt tcgcggtcgc ggcttatgtg
     1201 gtgatggacg ggttcgatct cggcatcggc atcctctttc ccctcttccg ggtggggaag
     1261 gagcgcgacc aggcgatgaa tgcggtggcg ccggtctggg acggcaacga gacctggctg
     1321 gtgatgggcg gaggcggcct actcgcggcc ttcccgctcg cttatgcgat cgtgctgccg
     1381 gcgctttacg cgcctttgat cgcgatgctg ctcggcctcg tcttccgcgg cgtcgccttc
     1441 gagttccgct ggcgcgaccc cggccaccgc ccgctctggg acgcgggctt ctttctgggg
     1501 tcgctggttg cggccttcgc ccagggtatc actttgggcg ccctgctcca gggcatcagc
     1561 gtagagggcc gcgcctatgc gggcggctgg tgggaatggc tctcgccctt cagcctgctc
     1621 accggcggcg ctctggtgat cggctacgcc ctgctcggcg cagcctggct gatctggaag
     1681 acgcaagggg ggatccagca ggatgcccgc cgcgccgcca cctggct
//
LOCUS       DASWNU010000231         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_501815, whole genome shotgun sequence.
ACCESSION   DASWNU010000231 DASWNU010000000
VERSION     DASWNU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501815"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VGB57_04535"
     CDS             complement(<1..452)
                     /locus_tag="VGB57_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0130652.1"
                     /translation="MGDPDSGLVFAAAAGDRAAFAELLKRHYDRIHALAWQLTGSRAD
                     ADDIAQDVCCTLVEKIGSFRGEAKFTTWLCGIVFNACRDLRRRRRSLSGLTERLAVLA
                     GLARAPDGRDLYDSIWLRSAIARLKPALRDAAVLVAGQQLSHGEAAEIL"
     gene            576..815
                     /locus_tag="VGB57_04540"
     CDS             576..815
                     /locus_tag="VGB57_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130653.1"
                     /translation="MIRVADAMEVESERGHIAAKRKSFFLRRAFAPSRLCAFYQKPGG
                     ETVEVQAGTSGRVQGEAGKPHIRHLFFRRSAWLFS"
     gene            complement(918..1658)
                     /locus_tag="VGB57_04545"
     CDS             complement(918..1658)
                     /locus_tag="VGB57_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0130654.1"
                     /translation="MHLNPTSGPLDLLLRKLQTHARLEEEDRRAIAALPYTLKTLEPQ
                     SYTVREGEAPEICAVLVSGFAYRQKLTGEGARQIVGIHIPGEALDLQNLFLEVSDHNV
                     QVLTRAEVAIVPRPALQELVRTRAAVGHAVMMTILVEASIFREWVLNVGRRDASTRLA
                     HLLCEFAVRLEAQGLANGDGFELPMTQEQLGDALGLTPVHVNRTIKGLEALGFVERDR
                     RRIRFPDWERMRDFGDFTQRYLHIGAGA"
     gene            complement(1819..>2040)
                     /locus_tag="VGB57_04550"
     CDS             complement(1819..>2040)
                     /locus_tag="VGB57_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130655.1"
                     /translation="HLRESGDVVVDEEGRDLPNLAAAAAAARANARCVIAGEAMTGKL
                     PLAACIEVHDEAGQRVLDLPFRDAVILDG"
BASE COUNT          328 a          702 c          687 g          323 t
ORIGIN      
        1 aggatttcgg ccgcctcgcc atggctgagc tgctggccgg cgacgagcac cgccgcgtcg
       61 cgaagcgcgg gcttcagccg ggcgatggcg ctcctcagcc agatcgaatc gtaaaggtcc
      121 cgcccgtccg gcgcccgagc caggccggca agcaccgcca gccgctccgt gaggccgctc
      181 agcgagcgcc gccgccgccg aaggtcgcgg caggcgttga agacgatccc gcacagccag
      241 gtcgtgaact tcgcttcccc ccggaagctt ccgatcttct cgaccagcgt gcagcacacg
      301 tcctgggcga tgtcgtcggc gtcggcccgc gaccccgtca gctgccaggc cagggcgtgg
      361 atccggtcgt aatgccgctt cagaagctcg gcgaacgccg cccggtcccc cgccgccgcg
      421 gcgaagacca gccccgaatc cggatcaccc accgctcacc gcccgccgcc aggcggggca
      481 ggggcgacgg gcccgcgccc cgtcacccgc catcggccga agctgcccct ccataaaccg
      541 tcccgccgcc cctcatgccc gctggacgct gatgcatgat tcgcgtggcc gatgccatgg
      601 aggtcgaatc tgagaggggc cacattgccg cgaagcgaaa atccttcttc ctacgtcgtg
      661 ccttcgcgcc ttcgcgcctt tgcgccttct atcaaaaacc gggtggcgag acggtcgagg
      721 tgcaggcggg tacgtcgggc cgagtgcagg gcgaggccgg caagccacac atccggcatc
      781 tcttcttcag gcgaagcgcc tggctgtttt cctgagagaa ggcgcgaagg cacaacgtag
      841 aaaaagaggg gggattggcg cttcgcggcg gtcttccgga aggacggttt cggctgagcc
      901 gcccgcctcg gcggcgctta cgcccccgcc ccgatgtgta gatagcgctg ggtgaagtcg
      961 ccgaagtcgc gcatccgttc ccagtcggga aagcggatcc ggcgccggtc gcgctcgacg
     1021 aagccgaggg cctcgaggcc ctttatggtc cggttgacgt ggaccggcgt gaggccgagc
     1081 gcgtcgccga gctgctcctg ggtcatcgga agctcgaagc cgtcgccgtt ggcgagcccc
     1141 tgcgcctcga gccggacggc gaactcgcag aggagatggg cgagccgggt cgaggcgtcg
     1201 cggcggccga cgttgagcac ccactcgcgg aagatgctgg cctcgaccag gatcgtcatc
     1261 atgacggcgt ggccgacggc cgcccgggtg cggaccagct cctgcagggc cgggcgcggg
     1321 actatcgcca cctccgcccg ggtaagcacc tggacgttgt ggtccgacac ttcgaggaaa
     1381 aggttctgga ggtcgagggc ctcgcccgga atatggatcc cgacgatctg ccgggcgcct
     1441 tcgccggtga gcttctgccg gtaggcgaag cccgacacga ggaccgcgca tatttccggg
     1501 gcctctccct cccgcaccgt gtagctctgc ggctccagag tcttcagggt gtagggcaaa
     1561 gccgcgatcg cccggcggtc ttcctcttcc agccgggcgt gggtctggag cttgcgcaga
     1621 aggaggtcga gcggaccgga agtggggttc aagtgcatca ggggttcccg cgtgagaagg
     1681 cgggagcacg aagcatctct caggcgcctc tgccgacaac gtccgaggcg ataccttcag
     1741 aatgatccat gttagataaa gttcctatct tatgttggcg agatcgccct caacataggt
     1801 tcgcggtcca ggggcggctt agccgtcgag gatcaccgcg tcccggaacg gcaggtcgag
     1861 cacgcgctga cccgcctcgt catggacttc gatgcaggcg gccagcggca atttgcccgt
     1921 catcgcctcg ccggcaatca cgcagcgcgc attcgccctc gcggccgctg ccgcggcggc
     1981 gagattgggc agatcccgcc cttcctcgtc gacgacaacg tccccgcttt ccctcaggtg
//
LOCUS       DASWNU010000232         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_86437, whole genome shotgun sequence.
ACCESSION   DASWNU010000232 DASWNU010000000
VERSION     DASWNU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86437"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..666)
                     /locus_tag="VGB57_04555"
     CDS             complement(10..666)
                     /locus_tag="VGB57_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0130656.1"
                     /translation="MPRTLEPAGYLVREGEAPEMCALLLSGFAYRHKVTGEGERQIIA
                     VHMRGEFLDLQNSFLEVADHNVQALTRCEIAAVPVPALRKLAEEYPRVGKAMWVDTLI
                     DAAIFREWIVNVGRRDSISRISHLLCEFALRLEAAGLSEEHRYEMPMTQEQIADCTGL
                     TPVHVNRVLKELGRLGLIDRDKRSVAIVQWDRLQHIGDFNTRYLHLEAGGPTNLASAR
                     "
     gene            977..1744
                     /locus_tag="VGB57_04560"
     CDS             977..1744
                     /locus_tag="VGB57_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0130657.1"
                     /translation="MVSTQIDDRALATPLVAKLQTRARLSDQDRDDLHSLYADARDIG
                     ARRSIIREGDRPDHVHLMVEGWAARYKLLPDGARQITAFLIPGDFCDLHVTILGEMDH
                     GIATLTRSKVAFIPRAQIEALTERPSLAKAFWWSTLVDEAVLRSWIVNIGRRDAFEAV
                     GHLICELYVRMKNIGLADGHRFELPLTQEEIGDALGLTSVHVNRVLQRLRSEDLISFR
                     HGLLTIHDYRTLEKASGFNPNYLHMDRRIDKPTSINQ"
     gene            1797..2042
                     /locus_tag="VGB57_04565"
     CDS             1797..2042
                     /locus_tag="VGB57_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130658.1"
                     /translation="MPRFFFHIYDDVVLKDEDGLELPDAEAARRTAVAGARAMMCDQL
                     TKGRLSLHHRIEVEDEQGGPVLALAFGDAVEIETAAG"
     gene            2231..5095
                     /locus_tag="VGB57_04570"
     CDS             2231..5095
                     /locus_tag="VGB57_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0130659.1"
                     /translation="MRFVAMKAALLAVTAAAAAGLAGAGAALSAAPAANRIEVPPLQY
                     KERRLANGLRIISMRDTSTANVSVSVWYEVGSKHDPEGRSGFAHLFEHILSRKTRNMP
                     YNMINRLVEDVGGDRNASTSTDRTNYYESVPARYLETMLWTHAERMARPVIDPQVFET
                     ERNVVKEEYRQGVLTPPYGRLRLLVTDNAFDISPHRRSTIGSIEQLDAATVADARAFH
                     EAYYGPDTATLIVSGNFDQKQLDSWVDKYLGQIPARAGKMPLAISVRDKPRTRPRTAT
                     MYAPNVPLPVIGSVWKIPGLAHLDTAAIEVLDGILTTGESSRLHKSLVREKRIATEAG
                     AQLASFEEEGYYAPNVILAGGRTVEEAEAALAAEIERVRNSPVTAAELAEAKNELLAQ
                     ALRQRETFSGRASGLGEVLVRSGDPRQWDKLIAAWQRVTAADVQRVARTYLTPASRVD
                     LRYLNESARPAGQADAWTNPAPMPRFKSAPPATRPPNELAAEGEREAPPAPGAAVRVT
                     PPAISETKLPTGLTVVTARSGQVPLATMTLVVKGGASTDPRGKAGLATMAAELATRGT
                     ASRSAEQISAELEALGATLNTGAGADGSFLSITAPVANLEAAGRVLADIAQNATFPAD
                     EIELQRKQAIDSLSIALRNPGQLAAMVALPLTYGTAPYGTLGNGTPRSLAALSRADFL
                     EQHRKWWHPGNSALVISGGIDSARADALAASLLGGWRGVGPAPTPPAERAGQPQKVRT
                     VVVDLPGAGQAAVLAAVRGISRSDPLYYQANAANAVLGGGSSGRLFQEVRTKRALSYG
                     AYSGMPARLDAGLLTASGQTKNETAAEVARVFLDELDRLGKQPLERQEVANRIAFITG
                     AFNRQAETSGGLGGMLANLLQQGMPPSEVARFVANMESVTPEAASAAATRLVGADRAT
                     LVIVGDSAKFIDKLRAVRPDVEVIRFDELDLDSPTLRKGG"
BASE COUNT          877 a         1940 c         1736 g          826 t
ORIGIN      
        1 gggacgacgc taccgcgccg aggcgaggtt ggtcgggccg ccggcttcga ggtggagata
       61 gcgggtgttg aagtcgccga tatgctggag ccggtcccac tggacgatgg cgaccgagcg
      121 cttgtcgcgg tcgatcaggc ccagccggcc aagctccttg agcacccggt tgacgtggac
      181 tggggtgagg ccggtgcagt cggcgatctg ctcctgggtc atcggcattt cgtagcgatg
      241 ctcctcggag agcccggccg cttccagccg aagcgcgaac tcgcacaaca gatgggagat
      301 ccggctgatc gaatcccgtc gcccgacgtt gacgatccat tcgcggaaga tggcggcgtc
      361 gatcagcgtg tcgacccaca tcgccttgcc gacgcgggga tattcctccg ccagcttgcg
      421 cagcgcgggc accggcaccg ccgcgatctc gcagcgggtc agcgcctgga cgttgtggtc
      481 ggcaacctcg aggaagctgt tctgaaggtc gaggaactcg ccccgcatat ggaccgcgat
      541 gatctggcgc tcgccctccc ccgtcacctt gtggcggtag gcgaagccgc tgagcaggag
      601 cgcgcacatc tccggagcct cgccttcgcg gaccagatag ccggcgggct cgagcgttcg
      661 gggcatgaag ggaagggcgc gaattgccgc ccgatcctca tcgtccagcg gcccgcgcgt
      721 ttcgaacctc cgcaggacgc gctcgacacc ttgagggaca ggcatttttt tcgctctccg
      781 cgcggcagat aaagggcggg agcttcgtat aatctctcgg ccaccgatgc cccgttgacg
      841 taccggtgat gctttcgttt ctaaccgatg cgtcgccaca agtatctcac ctgcgataga
      901 cacatgatgt ttaacatgga ttaggtttcg cgcacaatgc cgtggaccct gtgcgtcctg
      961 ccctgtactc aaacccatgg tctcaaccca gatcgacgac agggccctgg caaccccttt
     1021 ggtcgcgaag ctccagaccc gggcccgcct gagcgaccag gaccgcgacg atctccactc
     1081 gctctatgcc gatgcccggg acatcggagc gcgccggagc atcatccgcg aaggcgaccg
     1141 gcccgaccat gtccatctga tggtggaggg ctgggcggcg cgctacaaat tgcttccgga
     1201 cggagcccgc cagatcaccg ccttcctcat ccccggcgat ttctgcgacc ttcacgtcac
     1261 catcctgggc gagatggacc acggcatcgc gactctcacc cgctccaagg tcgccttcat
     1321 cccgcgcgcg cagatcgagg cgctcaccga gcgaccgtcg ctggccaagg ccttctggtg
     1381 gtcgaccctg gtcgacgagg cggtgctgcg cagctggatc gtcaacatcg gccgccgcga
     1441 cgccttcgag gcggtcggcc acctgatctg cgagctctac gtgcggatga agaatatcgg
     1501 cctggccgac ggtcatcgct tcgagctgcc gctcacccag gaggagattg gcgacgcgct
     1561 cggccttacc tccgttcacg tcaaccgggt gcttcagcgg ctgcgctcgg aggatctgat
     1621 cagcttccgg cacgggctgc tcaccatcca cgattatcgc acgcttgaga aagcctcggg
     1681 cttcaacccc aattacctgc acatggaccg gcgcatcgac aagccaacct cgatcaatca
     1741 gtgacttcct aaaccatgta gtcgcccccc cccccgcgaa tcgctaaggg ccgcgggtgc
     1801 cccggttttt cttccatatc tatgacgacg tcgtcctgaa ggacgaggac ggcctcgagc
     1861 tgccggatgc ggaggcggcc cgacgcacgg cggtggccgg cgcccgggcg atgatgtgcg
     1921 accagctcac gaagggccgg ctcagcctcc accaccggat cgaggtcgag gacgaacagg
     1981 gcggccccgt cctcgccctc gccttcggcg acgcggtcga gattgagacc gccgccgggt
     2041 gatatccgtc atcccggcga aagccgggac ctcggggaga cccgccggga cttcttgtcc
     2101 cgaggtcccg gctttcgccg ggatgacgga aggccgccca aacaagcaaa gcctgcatcg
     2161 aaagcccact ggcgccgctc gtccgcgcga actatccctc tgttccaaag ggatttgagg
     2221 ggagaaggca atgcgtttcg tggcgatgaa ggcggctctg ctcgccgtga cggcggcagc
     2281 ggcggcggga ctggccgggg ccggagcggc cctttcggcg gcacccgcgg cgaaccgcat
     2341 cgaggtcccg ccgctccagt ataaggaacg ccggctcgcc aacggcctcc ggatcatctc
     2401 gatgcgcgac accagcaccg ccaacgtctc ggtgtcggtc tggtacgagg tcggctccaa
     2461 gcacgatccg gaaggccgct ccggcttcgc ccatttgttc gagcacatcc tgtcgcgcaa
     2521 gacccgcaac atgccctaca acatgatcaa ccggctggtc gaggacgtcg gcggcgaccg
     2581 caacgccagc acgtccaccg accggaccaa ttattacgag agcgtcccgg cgcgctatct
     2641 cgagacgatg ctgtggaccc atgccgagcg gatggcgcgc cccgtcatcg atccgcaggt
     2701 gttcgagacc gagcgcaacg tcgtcaagga ggagtatcgc cagggcgtcc tcaccccgcc
     2761 ctacggccgg cttcgtctgc tggtcaccga caatgccttc gacatctcgc cccaccgccg
     2821 ctcgacgatc ggaagcatcg agcagctcga cgccgccacc gtcgccgacg cccgcgcctt
     2881 ccacgaagcc tattacggtc cggacacggc gaccctgatc gtctccggca atttcgacca
     2941 gaagcagctc gactcctggg tcgacaaata tctcgggcag atcccggcgc gcgccggcaa
     3001 gatgccgctc gccatctcgg tccgcgacaa gccccgcacc cggccccgca ccgcgacgat
     3061 gtacgcgccc aacgtgccgc tgccggtgat cggatcggtc tggaagatcc ccggattggc
     3121 gcatctcgac acggccgcga tcgaggtgct cgacgggatc ctgaccaccg gcgagagctc
     3181 caggctccac aagagtctgg tccgcgagaa gaggatcgcc accgaggcgg gcgcgcagct
     3241 cgcgagtttc gaggaggaag gctattatgc gccgaacgtg atcctcgccg gcggccgaac
     3301 ggtcgaggag gccgaggccg ctctcgccgc cgagatcgag cgggtccgca attcgccggt
     3361 caccgcggcg gagctcgccg aggcgaagaa cgagcttctc gcccaggcgc ttcgccagcg
     3421 cgagacgttc agcggccgcg cctcggggct cggcgaggtg ctggtccgat cgggcgaccc
     3481 ccggcaatgg gacaagttga tcgccgcttg gcagcgagtc accgccgccg acgtccagcg
     3541 tgtcgccagg acctacctca cgcccgcttc ccgggtcgac ctgcgctacc tcaacgaaag
     3601 cgcgcggccg gcgggccagg ccgatgcctg gaccaacccg gcgccgatgc cccgcttcaa
     3661 gtcggcgccg cccgcgaccc gcccgcccaa cgaactcgcc gccgagggcg agcgcgaagc
     3721 tccgccggcg cccggcgcgg cggttcgggt cactccgccg gccatctccg agacgaagct
     3781 cccgaccgga ctcaccgtcg tcaccgcgcg cagcggccag gtgccgctgg cgaccatgac
     3841 cctggtggtg aagggcggcg cctcgaccga cccgcgcggc aaggccgggc tcgcgacgat
     3901 ggccgccgag cttgcgacga ggggcacggc cagccgaagc gccgagcaga tctccgcgga
     3961 gctggaagcg ctcggcgcca ccttgaatac cggcgcgggc gccgacggat ccttcctttc
     4021 gatcaccgct ccggtcgcca atctcgaggc ggccgggcgg gtcctcgccg acatcgccca
     4081 gaacgccacc ttcccggccg acgagatcga gctccagcgc aagcaggcga tcgactcgct
     4141 gtcgatcgcg ctccgcaatc ccggccagct ggccgccatg gtcgctttgc ccttgaccta
     4201 cggcaccgcc ccttacggga cgctcggcaa cggcacgccg aggtcgctcg ccgcgcttag
     4261 ccgcgcggac ttcctcgagc agcaccgcaa atggtggcac cccggcaaca gcgccctcgt
     4321 catttcgggc gggatcgatt cggcgcgcgc cgacgccttg gcggcctcgc tgctgggcgg
     4381 ctggcggggc gtcgggccgg cgccgactcc gccggccgag cgcgccgggc agccgcagaa
     4441 ggtacgcacc gtcgtcgtcg acctgcccgg tgccggccag gcggcggtgc tcgcggcggt
     4501 gcgcggcatc agccgctccg atccgctcta ttaccaggcc aacgccgcca acgcggtgct
     4561 cggcggcggc tcgagcggac ggctgttcca ggaagtgcgg accaagcgcg cgctctccta
     4621 cggcgcctat agcgggatgc cggcgcggct cgacgccggg cttctcaccg ccagcgggca
     4681 gaccaagaac gagaccgcgg ccgaggtcgc cagggtcttc ctcgacgagc tcgaccggct
     4741 cggcaaacag ccgctcgagc ggcaggaggt cgccaatcgg atcgccttca tcaccggcgc
     4801 cttcaaccgc caggcggaga ccagcggcgg tttgggcggg atgctcgcca acctcctcca
     4861 gcagggcatg ccaccctcgg aagtcgcccg cttcgtcgcc aacatggaat cggtgacccc
     4921 ggaggccgcc tcggccgcgg ccacccgcct ggttggcgcc gaccgcgcca ccctggtgat
     4981 cgtcggcgac tcggccaagt tcatcgacaa gctccgcgcc gtgcgcccgg acgtggaggt
     5041 gatccgcttc gacgagctcg acctcgactc gccgacgctt cggaagggcg gctagcgccg
     5101 cctcaccccg tcatcccggc gaaagccggg atccatgaac acggcgctcg agttttccac
     5161 ctgcggtgtt catggattcc ggccttcgcc ggaatgacga ctgggccctg ttcaggccgc
     5221 gatcatcgca ttgcgcctag gctggcccct cacgccagct tagggtccgc tcatgcgcct
     5281 gttcctcgtc ccgctcgccg ccctcgtcct gcccggctgc atcgccagga cggcgctcga
     5341 cgtcgccacc ctgccggtca aggtggcctc ggcaggggt
//
LOCUS       DASWNU010000233         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_137887, whole genome shotgun sequence.
ACCESSION   DASWNU010000233 DASWNU010000000
VERSION     DASWNU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137887"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VGB57_04575"
     CDS             <1..428
                     /locus_tag="VGB57_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY0130660.1"
                     /translation="GSGVAVLCGRFEGMDERLFEARPVVPVSVGDYVLSGGETAAFVL
                     LDACVRLLPGVMGAPTSGEEESFEADLLEYPHYTRPVLWEGRTIPEVLRSGDHARIAA
                     WRQAQAMSDTRSRRPDLIGSHRDSRRPPPSGAQREEESD"
     gene            431..856
                     /gene="rplS"
                     /locus_tag="VGB57_04580"
     CDS             431..856
                     /gene="rplS"
                     /locus_tag="VGB57_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY0130661.1"
                     /translation="MNLIQQLEAEAIEQSVQGRPIPEFRPGDTVRIGVRVVEGERTRV
                     QNFEGVCIARANRGLGSSFTVRKISFGEGVERVFPLYSPNIETIEVVRRGAVRRAKLY
                     YLRGRTGKAARIAERRDTRPDKAAAAAKTATPETETAEG"
     gene            complement(951..3164)
                     /locus_tag="VGB57_04585"
     CDS             complement(951..3164)
                     /locus_tag="VGB57_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0130662.1"
                     /translation="MKRTGLIVALLLLALLGVMGMKSTLVPLPPASGAGAGGFDTARA
                     MARLQRILGDQRPHPVDSAANDEVRSRLVTELRALGLTPRIADTFTCNGAASAQTVSC
                     ARIRNVVATIGPAEGRHVLLVSHYDSTPTGPGAADDGIGIASMLEVAALLKDRPLKRP
                     VSLLFNEGEETGLIGARAFLDKDPLAARVDSLVNLESRGTTGPAIMFETSRPNGQAIR
                     HYARSVTRPAANSLTTDFSRLIPNQTDVAVLQERPWTILNFAIIGNETRYHSPDDLIA
                     NLDPRSLRHMGEQALQATADLVGKDAAPAGGELLYGDVLGIGLVTLPLWFGLALLAAL
                     LLGFAWLAWSRRGGVGRGAAAVALAMADAALTALLLHWLIGWLRPGEYWRAHPEWISL
                     AIDATAMASAAVTLLWIARPVARERLRLAFWLVFLILGAGICLVAPGAAIYFIVPPLL
                     ACVGLLRPRIERIATGLALLALSVHWLPLLHLSQVLLDFDAGWVFAPVSVLILFPFLI
                     EMKGLMARLGRERVTAVMATAAALAWLPAAFAPAYSIDRKQRLSLEYVWDEAQGKARW
                     MAYHDRGPLPEAMEALGEVERGVEVPWSSYSRWAADASGPAVAPPALERLDERAVGGN
                     RLVSFRLRMRGSDVVRIVGPKGTRFLAARAGGSFRRFGVDDSEEAPVFRCHGRSCDGL
                     AVTLLVEGTAPLDATVVGSRSGLPPEGSALVRGRPPNAQPQYVPDTTVAVAKVRL"
     gene            3235..4086
                     /locus_tag="VGB57_04590"
     CDS             3235..4086
                     /locus_tag="VGB57_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130663.1"
                     /translation="MTAMWQWLAALAAIAGAGAAQMAADVSSPLNDYNLSYDGAERIR
                     VFARSEADFANAMIYVSGRKSGRWSLPEPIGFSDPRYSDTDPWLTPDGRTLYFASNRP
                     TAERPGKKDLDLWRSRRSRDGRWSAPEHLGPAVNGPGPELGPEYHGGILYFGSARKNG
                     RGGLDIYAARGRDGAFPDAPELLSGPFNTAASESDFTLSPDGRHAVFWRMIGAKGVLH
                     IAGRTGAGWSVPVPLPEGVNIGPFNFTPSFARDGRTLRFASTRARPGQAEGMADIYVV
                     KLGGMPN"
     gene            4112..>4939
                     /locus_tag="VGB57_04595"
     CDS             4112..>4939
                     /locus_tag="VGB57_04595"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY0130664.1"
                     /translation="MSYRVVVVGATGNVGREMLNILAEREFPLDEIAAVASPRSTGDI
                     IDFGDSGTELKVRNIEHFDFAGWDMALFAAGSAVSREYAHKAAAAGCTVIDNSSLYRM
                     DPDVPLIVPEVNAHAIDGYRRKNIIANPNCSTAQMVVALKPLHDAAGIKRVVVSTYQS
                     VSGAGKKGMDELFEQSRNIFVGDSNEAAVFTKQIAFNVIPHIDEFLDDGSTKEEWKMV
                     VETKKILDPKIKVTATCVRVPVFVGHSESINIEFEREISAEDAQKILREAPGVMLVDK
                     "
BASE COUNT          839 a         1719 c         1648 g          733 t
ORIGIN      
        1 cggggtccgg cgtcgcggtc ctttgcgggc gcttcgaggg gatggacgag cgcttgttcg
       61 aggctcggcc ggtggttccc gtctcggtcg gcgactatgt gctctcgggc ggcgagacgg
      121 cggccttcgt cctcctcgat gcttgcgttc ggctgcttcc cggggtaatg ggcgcgccga
      181 cgagcggtga ggaggaaagc ttcgaagcgg accttctcga ataccctcat tatacccggc
      241 ctgttctatg ggaagggcgc acgatccctg aagtgctgcg atcgggggat catgcgagga
      301 tagctgcctg gcgccaagcc caggcaatgt ccgacacacg gtcaaggcgg ccggacctga
      361 tcgggagtca cagggactct cgaaggccac cgccctctgg cgcgcagcgc gaagaagaga
      421 gcgactagac atgaacctca tccagcagct cgaggccgag gccatcgagc agtccgtcca
      481 gggccgtccc attcccgaat tccgccccgg cgacaccgtc cggatcggcg tgcgggtcgt
      541 cgaaggcgag cgcacccgag ttcagaattt cgaaggcgtc tgcatcgccc gcgccaaccg
      601 gggtctcggc tcctccttca ccgtccgcaa gatcagcttc ggcgagggcg tcgagcgggt
      661 cttcccgctc tattcgccga acatcgagac gatcgaggtc gtgcgtcgtg gcgcggtgcg
      721 ccgggccaag ctctattatc tgcgcggccg taccggcaag gccgcccgga tcgccgagcg
      781 ccgcgacacc cgtccggaca aggctgccgc cgccgccaag acggccactc ccgagaccga
      841 gaccgccgag ggctgagctc tcgcgccata agacgccgaa agggggcggg gagggcaggg
      901 ttctccccgc cctttttcgt ctgcctctcg ttccgggtga cggagcgtcg ctagagccgc
      961 accttggcca ccgccaccgt tgtatccggg acgtattgcg gctgggcgtt cggcggcctc
     1021 ccccgtacca gcgccgaacc ttccggcggc agcccgctgc ggctgccgac gacggtcgcg
     1081 tcgagcggtg ccgtgccctc gaccagcaag gtcaccgcga ggccgtcgca gcttcgcccg
     1141 tggcagcgga agaccggcgc ctcctccgaa tcgtccaccc cgaagcgacg gaacgagccg
     1201 ccggcccgcg ccgccaggaa gcgggtgccc ttcggcccca cgattcgaac cacatccgag
     1261 ccgcgcatgc gcagccggaa ggaaaccagc cggttgccgc cgacggcgcg ttcgtcgagc
     1321 cgctccagcg ccggcggcgc gacggcggga ccgctcgcat cggccgccca gcgactgtag
     1381 ctcgaccagg gcacctccac cccgcgctcc acttcgccca gcgcctccat cgcctcgggc
     1441 agggggccgc gatcgtgata agccatccat cgcgctttcc cttgcgcctc gtcccacaca
     1501 tattcgaggc tgagcctctg cttgcggtcg atcgaataag cgggcgcaaa agccgccggc
     1561 agccaggcca gcgccgccgc cgtcgccatg acggcggtca cccgctcccg gcccagccgc
     1621 gccatcaggc ccttcatctc gatcaggaag gggaagagga tcagaaccga gaccggcgcg
     1681 aacacccagc cggcgtcgaa atcgaggagc acctggctga gatggaggag cggaagccag
     1741 tgcacgctga gggcaaggag cgccagaccc gtcgcgatcc gctcaattcg gggccggagc
     1801 agcccgacac aagcgagcag cggtggcaca atgaagtaga tcgccgcgcc cggggcgacc
     1861 aggcagatgc cggcgccgag gatcaggaag acgagccaga aagcgagccg cagccgctcg
     1921 cgcgccaccg gccgggcgat ccagagcaaa gtcaccgccg ccgaggccat cgccgtggcg
     1981 tcgatcgcaa gcgagatcca ctcggggtgg gcgcgccaat attcgccggg ccggagccag
     2041 ccgatcagcc agtggagaag caaggcggtc agagcggcat cggccatggc gagggcgacg
     2101 gccgccgccc ctcgcccgac cccgccgcgc ctcgaccagg ccagccaggc gaagccgagc
     2161 agcagcgccg cgagcagcgc caggccgaac cagagtggca gggtgaccag accgatgccg
     2221 agaacgtcgc cgtagagcag ctctccgccc gccggcgcgg catctttacc gaccagatcc
     2281 gccgtcgcct gcagcgcctg ctcgcccata tggcgaaggc tgcgcgggtc gagattggcg
     2341 atcaggtcat ccgggctgtg gtagcgggtc tcgttgccga tgatggcgaa gttgaggatc
     2401 gtccagggtc gctcctggag gacggccacg tcggtctggt tcggaatcag gcgcgaaaag
     2461 tccgtggtca gcgaattggc cgccggccgg gtcaccgagc gcgcataatg ccggatcgcc
     2521 tggccattgg gacggctcgt ctcgaacatg atcgcgggac cggtggtgcc gcgcgattcg
     2581 aggttcacca ggctgtcgac ccgcgccgcg agcggatcct tgtcgaggaa ggcgcgggcg
     2641 ccgatcagcc ccgtctcctc gccttcgttg aacagcaggc tcaccggccg cttcagcggc
     2701 cggtccttca gcaaggcggc cacctccagc atcgaggcga ttccgattcc gtcgtccgcc
     2761 gcgcccgggc cggtgggcgt ggaatcgtag tgagagacga ggagcacatg ccgcccctcg
     2821 gccgggccga tcgtcgccac gacattgcgg atccgagcgc aggagacggt ctgggcggac
     2881 gcggcgccgt tgcatgtgaa agtatcggcg atacgcggcg tcagccccag cgcccgcagt
     2941 tccgtgacga gccgactgcg cacttcgtca ttggcggcgc tgtccaccgg atggggccgc
     3001 tggtctccca gtatccgctg gagccgggcc attgcccggg cggtgtcgaa gcctccggct
     3061 cccgcccccg aggccggagg caacggcacg agggtgctct tcatccccat cacgccgagc
     3121 agcgccagca gcagcagcgc gacgatcagg ccggtccgtt tcatcgctct cccttggcac
     3181 gcgcccggcg ctcctgataa cagacggccg cggcgtggca acggggcggg aggaatgacg
     3241 gcgatgtggc agtggctcgc ggccttggcg gcgattgcgg gagcgggagc ggcgcaaatg
     3301 gcggccgacg tctcgtcccc tctgaacgat tacaatttga gctacgacgg ggccgagcgg
     3361 atacgggtct tcgcccgttc agaagccgac ttcgccaatg ccatgatcta cgtttccggg
     3421 aggaagagcg ggcgctggtc gctgcccgag ccgatcggct tctcggaccc gcgctacagc
     3481 gataccgatc cctggctgac ccccgacggc aggaccctct atttcgcgtc gaaccgcccg
     3541 accgccgagc gcccgggcaa gaaggacctg gacctctggc gctcccgccg gagccgagac
     3601 ggccgctggt ccgcacccga gcatctcggc cccgcggtca acggccccgg gccggagctc
     3661 ggccccgaat atcacggcgg catcctctat ttcggctcgg cccggaagaa cggccggggc
     3721 ggcctcgata tctacgccgc ccgagggcgc gacggcgcct tccccgatgc tccggagctg
     3781 ctttccggac cgttcaatac ggccgcgagc gaaagcgatt tcaccctctc ccccgacggc
     3841 cgccatgcgg tcttctggcg gatgatcggg gcgaagggcg tcctgcacat cgccggtcga
     3901 acaggcgccg gctggtcggt tccggttccg ctccccgaag gcgtcaacat cggcccgttc
     3961 aacttcacgc cgtcattcgc tcgcgatggc cgcaccctgc gcttcgcaag cacccgcgcc
     4021 cgccccggtc aggcggaggg gatggccgac atctacgttg taaagctcgg cgggatgcct
     4081 aactgaggtc cttgccttca ggagtaatga aatgagttat cgggtggtcg tcgtcggagc
     4141 gaccggcaat gtcgggcgcg agatgctgaa catcctcgcc gagcgggaat tccccttgga
     4201 cgagatcgcc gccgtcgcct cgccgcgctc gaccggcgac atcatcgact tcggcgacag
     4261 cgggaccgag ctcaaggtgc gcaacatcga gcatttcgac ttcgccggct gggacatggc
     4321 cctgttcgcc gccggctcgg ccgtgtcgag ggaatatgcg cataaggccg ccgccgccgg
     4381 ctgcaccgtg atcgacaaca gctcgctcta ccggatggac cccgacgttc cgctgatcgt
     4441 gcccgaggtg aacgcccacg ccatcgacgg ctatcgccgc aagaacatca tcgccaaccc
     4501 caattgctcg accgcgcaga tggtggtcgc cctgaagccg ctccacgacg cggccggaat
     4561 caagcgcgtc gtcgtctcga cctaccagtc ggtttccggc gctgggaaga agggcatgga
     4621 cgagctgttc gagcagagcc gcaacatctt cgtcggcgat tcgaacgagg cggccgtctt
     4681 caccaagcag atcgccttca acgtcatccc ccatatcgac gaattcctcg acgacggctc
     4741 gaccaaggag gaatggaaga tggtggtcga gaccaagaag atcctcgatc cgaagatcaa
     4801 ggtgaccgcc acctgcgtcc gggtcccggt cttcgtcggc cattcggaat cgatcaacat
     4861 cgagttcgag cgcgagatct cggccgagga cgcccagaag attctccgcg aggcgccggg
     4921 cgtcatgctc gtcgacaag
//
LOCUS       DASWNU010000234         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_86602, whole genome shotgun sequence.
ACCESSION   DASWNU010000234 DASWNU010000000
VERSION     DASWNU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86602"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..902)
                     /locus_tag="VGB57_04600"
     CDS             complement(177..902)
                     /locus_tag="VGB57_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0130665.1"
                     /translation="MIQVLLSEAAKLRRSLVLLLCAAAPVMVALLCALMFHQKGGSDQ
                     PWLSYLASASAIWSFFMLPMTVTALTILVAQLEHGPRFWNHLLALPVPRAHFFAAKLV
                     VVVVLVAAMTALLFVLTPAFGWLGDAVASERSLTGELALREFAEILAKMFAGSLLLIA
                     LQLWTALRFRSFVPPLVLGIGGTFVGVVATGSDYGPYFPWLIATNVLASEPARAQLAL
                     AVGLAGGLIAAAAMLVDLTRREH"
     gene            complement(914..1843)
                     /locus_tag="VGB57_04605"
     CDS             complement(914..1843)
                     /locus_tag="VGB57_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0130666.1"
                     /translation="MKLALETSALSRRFGRQQAVCGVDLAVPEGAVYGFLGQNGAGQT
                     TPIRMLLGLLRPSGGSARLFGHDVRRDRLAAARLTGALVETPCHYDHLTGRENLAITA
                     RLLRAAPTEIGRVLEIVELTGAADRRVGGYSLGMRQRLGVARALIGRPRLLILDEPTN
                     GLDPQGIRDMRRLIATLAEAEGVTVLVSSHILTEVEHTATHVGLMHQGRLLLQGPASA
                     LKSGRSKRIVFSVDRPEPVIEMLRDTGLAARLDGAGRVLIDPASADTADVAAVNFMLV
                     ELGIRVSGIEVREPSLEQIFHDTIGQADLLLAA"
     gene            1947..2486
                     /locus_tag="VGB57_04610"
     CDS             1947..2486
                     /locus_tag="VGB57_04610"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEY0130667.1"
                     /translation="MAGEKMERLRRHSLWLSRLTLFLFCATALVMLLPTLRGLAIAGP
                     GEAVGRNILFMSVIWFPGLFYLYALWAIGRSFGAFARGGVFGSAMATGCRRAGVALAL
                     GATSSAVGVPNAIRLLMQQGFFLDQGKRFGAVLIFDTAYLAVGVVGIALYLLGHLLER
                     AAEVQQEAQDLRAELGEFF"
     gene            2486..2731
                     /locus_tag="VGB57_04615"
     CDS             2486..2731
                     /locus_tag="VGB57_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130668.1"
                     /translation="MPVVVTLDSVLSARRMKAKELARIIGISETQLSLFRSGKVRGVR
                     FSTVAALCAVLECKPGDLLDYVHDPADVDRNAAAGED"
     gene            2791..>2953
                     /locus_tag="VGB57_04620"
     CDS             2791..>2953
                     /locus_tag="VGB57_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0130669.1"
                     /translation="MRPSGRAPDQMREIVMEAGFTRHAEGSCLISFGDTRVLCTASVE
                     QSLPPWLRGK"
BASE COUNT          474 a          994 c         1018 g          467 t
ORIGIN      
        1 tttcgatcgt ccaggcggag atcggcttgc cccagctgtt cgcctcgaag gacaggctcc
       61 gcaccgcgac gggcggctcc gtcccttccg gtcccggcgc cgcgcagccc cccaacagcg
      121 ccaggatcaa aaccgctcgc ctcattccat tctcccgaag agccgtcgtc gcattcctaa
      181 tgctcccgcc gagtgaggtc cacgagcatc gcggcggcgg cgatcagccc gcccgccagc
      241 ccgaccgcca gcgccagctg agcccgcgcc ggctcgcttg ccagcacatt ggtggcgatc
      301 agccagggga aatagggccc gtagtcggag ccggtggcga cgactccgac gaaggtgccg
      361 ccgatgccga gcacgagggg tggaacgaag ctccggaagc gaagggccgt ccacagctgc
      421 agcgcgatga ggagcaggct gcccgcgaac atcttcgcca ggatttcggc gaattcccgc
      481 agtgcgagct cgccggtcag cgagcgctcc gatgcgacgg cgtcgccgag ccagccgaac
      541 gccggagtca gcacgaagag caaggccgtc atggccgcaa cgagcaccac cacgacgacc
      601 aacttggccg cgaagaagtg ggcgcgcggc accggcaggg ccagcaggtg gttccagaat
      661 ctcgggccgt gctcgagttg cgcgacgagt atggtgagcg ccgtgaccgt catcgggagc
      721 atgaagaagc tccagatggc ggaggcgctg gccaggtagc tcagccaggg ctgatcggac
      781 ccccctttct ggtggaacat caaggcgcac agcaaggcga ccatcaccgg cgccgccgcg
      841 cagagcagga ggacgagcga gcggcgcagc ttggccgctt cggagaggag gacctggatc
      901 atctcggagc ctttcaggcg gcaaggagca ggtcggcctg cccgatcgtg tcgtggaaga
      961 tctgctcgag cgacggctcg cggacctcga tcccggagac ccggatgccg agctccacca
     1021 gcatgaagtt gaccgccgcg acgtccgcgg tgtcggcgga ggcggggtcg atgagcaccc
     1081 ggccggcacc gtcgaggcga gccgcgagac cggtgtcccg aagcatctcg atcaccggct
     1141 ccggccggtc gacgctgaag acgatccgct tgctccggcc gctcttcaac gccgaagccg
     1201 gcccctgcag gagcaggcgg ccctggtgca tcagcccgac gtgggtggcg gtgtgctcga
     1261 cctcagtgag gatgtggctg gagacgagca ccgtcacgcc ctcggcctcg gccagggtgg
     1321 cgatcagccg ccgcatgtcc ctgatgccct ggggatcgag gccgttggtc ggctcgtcga
     1381 ggatgaggag gcgcggccgg ccgatcaggg cccgggcgac tcccaggcgc tggcgcatgc
     1441 cgagcgaata gccgccgacc cgccggtccg ccgcgcccgt cagctcgacg atctccagca
     1501 cccggccgat ctcggtcggg gcggcgcgca gcagccgggc ggtgatcgcc agattctcgc
     1561 gaccggtgag atgatcgtaa tggcagggag tctcgaccag cgctccggtg aggcgggccg
     1621 ccgccagccg gtcgcggcgg acgtcgtggc cgaacagccg ggcgctgccg ccgctcggcc
     1681 tcagcaggcc gagaagcatc cggatcggcg tcgtctggcc ggcgccgttc tggccgagga
     1741 agccgtagac ggcgccctcg ggaaccgcga gatcgacccc gcacaccgcc tgctgccgcc
     1801 cgaaccggcg gctgagggcg ctcgtttcca aggccagctt cattttaacc tccccacgtc
     1861 gaccgaaagc tcgactcatc ccctgtcgtc atttaagtta gatatggatt tatttaactg
     1921 caacataaac ttccggggag cgaaggatgg cgggtgagaa gatggagcgg ctgcgccgcc
     1981 acagcctctg gctgtcgcgc ctcacccttt tcctgttttg cgcgaccgcc cttgtcatgc
     2041 tgcttccgac gctcaggggc ctggccatcg ccgggcccgg cgaagccgtg ggccggaaca
     2101 tcctgttcat gtcggtgatc tggtttccgg gcctcttcta tctttacgct ctctgggcga
     2161 tcggccgctc gttcggagcc ttcgcgcgcg gcggtgtgtt cggctcggcc atggcgaccg
     2221 gatgtcgaag ggccggagtg gcgctcgcgc tcggagcgac gtcgagcgcg gtgggcgtgc
     2281 ccaacgcaat tcgcctcctg atgcagcagg gtttcttcct cgatcagggg aaacgtttcg
     2341 gtgccgttct gatattcgac accgcctacc tcgccgtcgg agtggtcgga atcgcgctct
     2401 atctgctcgg gcacttgctg gagcgcgcgg ccgaggtgca gcaggaggcg caggaccttc
     2461 gcgccgagct gggcgagttc ttctgatgcc ggtcgtagtc actctcgact ccgtcctttc
     2521 ggcgcggcgg atgaaggcga aggagctcgc ccggatcatc ggcatcagcg agacccagct
     2581 gtcgctcttc cgctccggca aggtgcgcgg cgttcgcttc tcgaccgtcg cggccctgtg
     2641 cgcggtgctg gaatgcaagc ccggcgacct gctcgattac gtccacgatc ctgccgacgt
     2701 ggaccgcaat gccgcggcgg gcgaagacta gcgggtggat tgtccgcgcc gctctggcta
     2761 aggcccggct ttcgatttga aaggagcaac atgcgcccat ccggacgcgc gcccgaccag
     2821 atgcgggaga tcgtgatgga ggcgggcttc acccgccacg ccgagggcag ctgcctgata
     2881 agtttcggcg acacccgggt gctgtgcacc gcctcggtcg agcagagcct tccgccctgg
     2941 ctgcgcggca agg
//
LOCUS       DASWNU010000235         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_449173, whole genome shotgun sequence.
ACCESSION   DASWNU010000235 DASWNU010000000
VERSION     DASWNU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449173"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VGB57_04625"
     CDS             complement(<1..448)
                     /locus_tag="VGB57_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0130670.1"
                     /translation="MNRLRPILLAAALALPASPAFAEDAARPRESVAAGLSSEQRSGY
                     REVFAAIRANDWLGASARLAALPEGPLHNVARAELFLAKGSPQVEAPDLISLLQRAPE
                     MPKAEQIARLAQTRGATELPGIPEAQKLVWVGSQPRRSRAEATKGDQ"
     gene            complement(445..1125)
                     /locus_tag="VGB57_04630"
     CDS             complement(445..1125)
                     /locus_tag="VGB57_04630"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0130671.1"
                     /translation="MSWWTEAGVDTLVDEEPRDWLRPKAREAPPAAPIVDEAPGEALP
                     GQLDLFQAWLASSDRLAFAAPAAPRVCPAGDPNSGLMILADMPTGEDCTAGALISGEA
                     GRLFERMLAAIGRDRGSVYLAALSCIRSPSGQLNSEAMKSCAGLARHHIGLAAPRAVL
                     LFGDACAKALLGLSVPQARGRWHEIATHAGPVKALATIAPRQLLDSPRLKAMAWADLQ
                     MLIEEVKE"
     gene            1290..>2171
                     /locus_tag="VGB57_04635"
     CDS             1290..>2171
                     /locus_tag="VGB57_04635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0130672.1"
                     /translation="MSERESMPYDVVIVGAGPAGLAAGIRLKQLADEAGRELSVCIIE
                     KGSEVGAHILSGAVMDPRALEELLPNWRDLDSPLTVPVVENHHWVLTERKRYSLPHLM
                     MPPFMNNKGTYTGSLGNFCRWLAGQAEALGVEIFPGFAAAEVLFNEDGSVKGVATGDM
                     GVARDGTRRADYQPGMELHGRYTFFAEGARGHLTKQLKRTFDLDRESQPQVYGIGLKE
                     LWDIEPGKHVPGRVIHTQGWPLEDSAWGGGFLYHQENNQVALGFVVALDYSNPYLSPF
                     EEMQRWKTHPAVRGEIEG"
BASE COUNT          342 a          731 c          762 g          336 t
ORIGIN      
        1 cctgatcgcc cttggtcgcc tcggcgcgcg agcgccgcgg ctggcttccc acccagacca
       61 gcttctgcgc ctccggaatg cccggaagct cggtcgcgcc tcgagtctgg gcgaggcggg
      121 cgatctgctc cgccttgggc atttcgggcg cgcgttgaag gagggagatc aggtcgggtg
      181 cctcgacctg cggcgatccc ttggcgagga acagctcggc ccgcgcgaca ttgtggagcg
      241 gcccctccgg caaagcggcg agccgggccg atgcccccag ccagtcgttc gcccggatcg
      301 cggcgaagac ttcgcgatag ccgctccgct gctcggagct gagccccgcc gcaaccgact
      361 cgcgcgggcg ggcggcgtcc tcggcgaagg cgggggacgc cggcaaggcg agcgcggcgg
      421 ccagcaaaat ggggcggaga cggttcattc cttcacctct tcgatcagca tctggaggtc
      481 tgcccaggcc atggccttga ggcgcggcga atccagcaat tgtcggggag caatggttgc
      541 cagagccttg acgggcccgg cgtgggtggc gatctcatgc cagcggcctc gcgcctgcgg
      601 caccgacagg ccgagcaggg ccttggcgca ggcgtcgccg aagagcagga ccgccctggg
      661 cgcggccagc ccgatatggt gacgggccag gccggcgcag ctcttcatcg cctcgctgtt
      721 gagctgtcct gacggcgagc ggatgcagga gagcgcggcc agatagacgg agccgcggtc
      781 gcgaccgatg gcggcgagca ttcgttcgaa cagccgtccc gcctcgccgc tgatcaaggc
      841 gccggcggtg caatcctcgc ccgtgggcat gtcggcgagg atcatcagcc cggagttcgg
      901 atcgccggcc gggcagactc gcggagcggc gggcgcggcg aaggcgagac ggtcgctcga
      961 ggcgagccag gcctggaaca ggtcgagctg gccgggcagg gcctcgccgg gcgcttcgtc
     1021 gacgatcgga gcggccggcg gcgcctcccg tgctttcggc cgaagccagt cgcgcggctc
     1081 ctcgtccacg agcgtgtcga cgcccgcttc ggtccaccag ctcaacgcgc tttcggcgtc
     1141 ggccggcgaa aatgcatcgc ttccccgctc cactgcgcca tggagtcagc ctcgggcggg
     1201 ctggtcaacc tcaaattctc tatctatctg caagccgttg gctgcctgcg acgacgtgag
     1261 ttgcgggtgc ccgccacagg agcgtcttga tgtccgaacg cgagtcgatg ccgtatgacg
     1321 tggtgatcgt cggtgccggg ccggcaggcc tcgctgcggg cataaggctc aagcagctcg
     1381 ccgacgaggc ggggcgagag ctcagcgtct gcattatcga aaagggctcg gaggtcggag
     1441 cccatatcct gtccggcgcg gtcatggatc cccgagccct cgaggagctg cttcccaact
     1501 ggcgcgatct cgacagcccg ctgaccgtcc cggtcgtcga gaaccatcat tgggtgctca
     1561 ccgagcggaa gagatattcg ctcccgcacc tgatgatgcc gcccttcatg aacaacaagg
     1621 gcacctacac cggctcgctc ggcaatttct gccgatggct cgccggccag gccgaggcgc
     1681 tcggggtcga gatcttcccg ggcttcgcgg cggccgaagt gctgttcaac gaggacggtt
     1741 cggtcaaggg cgtggcgacc ggcgacatgg gggtcgcccg cgacggcacc cgccgggccg
     1801 actaccagcc cggaatggag cttcacggcc gctacacctt cttcgccgaa ggcgctcgcg
     1861 gccatctcac caagcagctc aagcggacct tcgacctcga ccgcgagagc cagccccagg
     1921 tctacggcat cggcctcaag gaattgtggg atatcgagcc ggggaagcac gtgccgggcc
     1981 gggtcatcca tacccagggc tggccgcttg aagacagcgc ctggggcggg ggcttcctct
     2041 accatcagga gaacaaccag gtcgccctgg ggttcgtcgt cgcgctcgat tattccaacc
     2101 cctatctctc gccgttcgag gaaatgcagc gctggaagac gcatccggca gtccgcggcg
     2161 agatcgaggg g
//
LOCUS       DASWNU010000236         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_449248, whole genome shotgun sequence.
ACCESSION   DASWNU010000236 DASWNU010000000
VERSION     DASWNU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449248"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="prfB"
                     /locus_tag="VGB57_04640"
     CDS             <1..1064
                     /gene="prfB"
                     /locus_tag="VGB57_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0130673.1"
                     /translation="LDWDRALRRLDELNARVEDQSLWNDPRAAQEVMRERRRLEEAIA
                     ATRAIQQELDDNVELLEMAEAEGDESMEGDAVAALAELAERAERDKVTALLAGEADSN
                     DTYIEVNSGAGGTESQDWAEMLHRMYRRWAERRGFKVEVVDYHSGEQAGIKSATLMVK
                     GENAYGYAKTESGVHRLVRISPYDSNARRHTSFSSVWVYPVVDDDIDIEVNEGDLKID
                     TYRASGAGGQHVNTTDSAVRITHLPSGIIVACQNQRSQHKNKAEAMKMLKARLYEAEL
                     QRREAQASAEHAAKTDIGWGHQIRSYVLQPYQLVKDLRTGVTSTAPGDVLDGDLDRFM
                     AAALSQRVTGEEVEVEDVD"
     gene            1186..1890
                     /locus_tag="VGB57_04645"
     CDS             1186..1890
                     /locus_tag="VGB57_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0130674.1"
                     /translation="MPGRIFGALLFPLLLLAACQEKTEESPFPRPQRPVSPIVSASYS
                     NEDARDSVREAETVMDLADIEAGMYVADIGAGEGYYTVRLAPLVGRKGRVLAQDIMPQ
                     TRDALASRVTREDLHNVAVKLGKPNDPLLPDASFDRVFMIHMYHEIERPSEFLWNLRP
                     DLKKNGRVVVVDADRPTDRHGTPPRLLICEFAAVGYQLTRFERLPDSESYFAQFEPAG
                     GRPEPKDIPVCPAVAG"
     gene            complement(1963..2601)
                     /locus_tag="VGB57_04650"
     CDS             complement(1963..2601)
                     /locus_tag="VGB57_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY0130675.1"
                     /translation="MVTRVKTAKGRKVGSTKWLERQLNDPYVKRAKAEGYRSRAAYKL
                     TELDDRFGFLKGVERVVDLGVAPGGWTQVVRKRVPKAAVVGIDLLPTDPIDGAVLLQL
                     DFLAEEAPERLREALGGPADLVLSDMAANTVGHPQTDHLRTMALVEAGLEFAGEVLRP
                     GGAFVAKVLAGGADNHLVAELKRRFTAVKHAKPPASRKGSSEWYVVAQGFKG"
     gene            complement(2744..>3309)
                     /locus_tag="VGB57_04655"
     CDS             complement(2744..>3309)
                     /locus_tag="VGB57_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY0130676.1"
                     /translation="IFDIGGGSTELVLIDTDGMTPKIKCWWSAPWGVVSLTESEGRDF
                     ADADERLAAYGRMKERVRHAFRRFAELLPPREGNIRLLGTSGTVTTLASVHLALPSYD
                     RRAVDGLMMPVDKMRAVNHMLSRMSFAERSELPCIGAERADLVVAGCAILEAIMDIWP
                     AQTLGVADRGIREGILRSLMAKDGHQL"
BASE COUNT          556 a         1148 c         1089 g          516 t
ORIGIN      
        1 tcctcgactg ggaccgggcc ttgaggcgtc tcgacgaatt gaacgcccgg gtcgaggacc
       61 agtccttgtg gaacgatccc agggcggctc aggaggtgat gcgcgagcgg cggcggctcg
      121 aggaagcgat cgccgccacc cgagccatcc agcaggagct ggacgacaat gtcgagcttc
      181 tggagatggc cgaagccgag ggcgacgagt cgatggaagg cgacgccgtc gccgcgctcg
      241 ccgagctcgc cgagcgggcc gagcgcgaca aggtcaccgc cctcctggcc ggcgaggccg
      301 attccaacga cacctatatc gaggtcaatt ccggcgccgg cggaaccgag agccaggact
      361 gggccgagat gctccaccgc atgtaccggc gctgggccga gcggcgcggc ttcaaggtcg
      421 aggtggtgga ctatcattcc ggggagcagg ccgggatcaa gtcggcgacg ctgatggtca
      481 agggcgagaa cgcctatggc tatgccaaga cggaaagcgg ggtgcaccgc ctggtccgca
      541 tcagccccta cgattccaac gcccggcggc acaccagctt ctcgagcgtc tgggtctatc
      601 cggtggtcga cgacgatatc gacatcgagg tcaacgaagg cgatctgaag atcgacacct
      661 accgggcgtc gggggcgggc ggccagcacg tcaacaccac cgacagcgcg gtcaggatca
      721 cccaccttcc ctccgggatc atcgtcgcct gccagaacca gcgctcgcag cacaagaaca
      781 aggccgaggc gatgaagatg ctcaaggccc ggctctacga ggccgagctc cagcgccggg
      841 aggcgcaggc gagcgcggag catgcggcca agacggacat cggctggggc caccagatcc
      901 gctcctacgt cctccagccc tatcagctgg tcaaggacct gcgcacggga gtcacttcca
      961 ccgcccctgg cgacgtgctc gacggcgatc tcgaccggtt catggccgcc gctctgtctc
     1021 agcgggtcac gggcgaggag gtcgaggtcg aagatgtgga ttaggtctct taaatcctcc
     1081 ccgcgatttc gcggggaggg ggaccaggcg aagcctggtg gaggcgttct tctgactcag
     1141 caaggacccc tccaccaccc tgcgggtggt ccccctcccc ggcaaatgcc ggggaggatt
     1201 ttcggcgccc tcctcttccc cctcctcctc ttggccgcct gccaggagaa gacggaggaa
     1261 tcccccttcc ccaggccgca acggccggtg tcgccgatcg tctcggcaag ctattcgaac
     1321 gaggatgccc gcgacagcgt ccgcgaggcg gagacggtga tggacctcgc cgacatcgag
     1381 gccggcatgt acgtcgccga catcggcgcc ggcgaaggct attatacggt ccggctcgcc
     1441 cccctggtgg ggcgcaaggg tcgggtgctc gcgcaggata tcatgcccca gacccgcgac
     1501 gcgctcgcca gccgggtcac ccgcgaggat ttgcacaatg tcgcggtcaa gctcggcaag
     1561 ccgaacgatc ccctgctccc cgacgcgagc ttcgaccggg tcttcatgat ccacatgtat
     1621 cacgagatcg agcggccgag cgagttcctg tggaacctgc gccccgatct gaagaagaac
     1681 ggtcgggtcg tcgtcgtcga cgccgaccgg cccaccgacc gccacggcac gcctccgcgg
     1741 ctgctcatct gcgagttcgc cgcggtcggc tatcagctca cccggttcga gcggcttccc
     1801 gattccgaat cctatttcgc ccagttcgag ccggccgggg gccgcccgga gcccaaggac
     1861 atacccgtgt gcccggccgt cgcgggctga tgctccccgg cgaaggccgg ggcccagttc
     1921 ttacgtcgat gccggtgggc cccggccttc gccggggagc aatcatccct tgaacccctg
     1981 cgccaccaca taccattcgg acgagccctt gcggctcgcc ggaggcttgg cgtgcttgac
     2041 cgcggtgaag cgccgcttca attccgccac gagatggttg tcggcgccgc ccgcgagcac
     2101 cttggcgacg aaagcgccgc ccggcctcag cacctcgccg gcgaattcga ggcccgcctc
     2161 gaccagcgcc atggttcgca gatggtccgt ctggggatgg ccgaccgtgt tcgccgccat
     2221 gtcggacagg acgaggtcgg ccggcccgcc gagcgcctcg cgaagccgct ccggcgcctc
     2281 ctcggcgagg aagtcgagct ggagcaggac ggcgccgtcg atcgggtcgg ttggaaggag
     2341 gtcgatcccc accaccgccg ccttcggcac ccgcttgcgg acgacctggg tccagccgcc
     2401 gggcgccacc ccgagatcga cgacccgttc cacgcccttc aaaaagccga aacggtcgtc
     2461 gagctcagtg agcttgtagg cggcgcggga gcgatagccc tccgccttgg cgcgcttgac
     2521 ataggggtcg ttgagctggc gttcgagcca tttggtcgag ccgaccttgc ggcccttggc
     2581 ggtttttacc cgggttacca ttttttgcct ccccgcgcgc agcgggggga gggggaccac
     2641 ccgtagggtg gtggaggggt aactggcaga agcccgcgcc aattacccct ccaccccgct
     2701 acgcggggtc cccctcccca tctccgatgg ggaggcaata tactcacaat tgatgtccgt
     2761 ccttggccat gagcgagcgc aatatgccct cgcgaatgcc ccggtccgcc acgcccaggg
     2821 tctgggccgg ccagatgtcc atgatcgcct cgaggatggc gcagccggcg acgaccaggt
     2881 cagcccgctc ggcgccgatg cagggcagct ccgagcgctc ggcgaagctc atccgcgaca
     2941 gcatgtggtt gaccgcacgc atcttgtcga ccggcatcat cagcccgtcc accgcgcggc
     3001 ggtcgtagct cggcaaagcg aggtggacgc tggcgagcgt cgtcaccgtt ccgctggttc
     3061 cgagcagccg gatattgccc tcgcgcggcg gcagcaattc ggcgaagcgg cggaaggcgt
     3121 ggcggacccg ctccttcatc cggccgtaag cggcgagcct ttcgtccgcg tcggcgaagt
     3181 cccggccttc gctctcggtc agtgagacca cgccccacgg cgcgctccac cagcatttga
     3241 tcttgggcgt catcccgtca gtgtcgatca gcaccagctc cgtcgatccg ccaccgatat
     3301 cgaagatca
//
LOCUS       DASWNU010000237         6859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_346147, whole genome shotgun sequence.
ACCESSION   DASWNU010000237 DASWNU010000000
VERSION     DASWNU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346147"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VGB57_04660"
     CDS             <1..651
                     /locus_tag="VGB57_04660"
                     /inference="COORDINATES: protein motif:HMM:NF020052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HEY0130677.1"
                     /translation="RPEPPDAPPPEPPTDEPPLPPREKLVIRLADGKERTFQHISGTT
                     FWDSSGKPISAQQFVEQLFGKLPELFRDEHELRRIWSDPDTRKALVAGLAERGFAGEQ
                     LQQIRRMIDGTQSDLFDVLRYIAFTKAPLKRAERASTRRDAILARWNVRQSEFLDFVL
                     SQYVSEGEEELGTDKLPDLLELKYGTPADAVRRLGSVSGIREAFRGFQKDLYGDDN"
     gene            720..1154
                     /locus_tag="VGB57_04665"
     CDS             720..1154
                     /locus_tag="VGB57_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130678.1"
                     /translation="MKATATRAAALAVLALALAGPAPAASAPPQAQQDELSTLIDRNL
                     RSGGSWFTAEERAVIEQKCGYAPGAWDGFEANMSDGTFTCRDGRKVDDPEVRAVLRAV
                     APRIEARVEAVMARADIVAAIARVAGVAAAEALREVEARHRR"
     gene            complement(1532..3070)
                     /locus_tag="VGB57_04670"
     CDS             complement(1532..3070)
                     /locus_tag="VGB57_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY0130679.1"
                     /translation="MSAFGRRPGNGGTGGGRPTFGIARPMKGPGGAVAPAVAPEGGDQ
                     FPPLDTISFSDSDTDSFTPTGDRGADAMSRLADRQAASSEAPSSKNEGFEASIHRIKE
                     QVLPRLLERVDPEAAATLSKDELAEEFRPIIGEVLAELKINLNRREQFALEKVLVDEL
                     LGLGPLEELLADPNISDIMVNGPNQTFIERKGKLEIAQIQFRDEEHLFQIAQRIVNKV
                     GRRVDQTTPLADARLQDGSRVNVIIPPLSLRGTAISIRKFSEKPITVDMMKNFGSMSD
                     KMATVLKIAGASRFNIIISGGTGSGKTTMLNALSKLIDPGERVLTIEDAAELRLQQPH
                     WLPLETRPPNLEGQGAISIRDLVINALRMRPDRIILGEIRGPECFDLLAAMNTGHDGS
                     MATLHSNSPRECLARMENMVMMSDIKVPKEAISRQIADSVDLIVQVMRLRDGTRRTTN
                     ITEVIGMEGDVIVTQELFKFEYLDETADGKIIGEYRSMGLRPYTLEKAKQFGFDQPYL
                     EACL"
     gene            3167..4063
                     /locus_tag="VGB57_04675"
     CDS             3167..4063
                     /locus_tag="VGB57_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0130680.1"
                     /translation="MPAQHARSALLIALAGFLSLAFGDALVKSMAGQWPAPAVAALRY
                     SFGAVGLTLYVALRHGREGFVMPRPWLHVGRGAAVALATLCFFLGVMAMPLADATAIQ
                     FTSPILTALLAPLVLGERTRPATWAATLLAFLGVLIVLRPNLLEIGPAALFPMGAAFG
                     MSWLMMFNRMAAGDAPVMVMQFLVAVVAAPLLIAAAAAFHASGVTIGWPSAVVVLKCL
                     GVAGFATAGHALIFAAVERASASTVAPMTYVQLLVAAFLGWLWFGDAPDAATFGGAAL
                     IIGGGLLLWRAQRVRHLREEPT"
     gene            complement(4039..4632)
                     /locus_tag="VGB57_04680"
     CDS             complement(4039..4632)
                     /locus_tag="VGB57_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130681.1"
                     /translation="MNIGTTLVAALLAPAPAASPPVETALASAARYCETVVTEKNVAP
                     PLPAGATVEPEGGVPDLVKRFAATQSMVSMFGVGAFAHFRARNGQVWVIRSPQPAVCH
                     IMVTAVPGGSAALGASFTKSLAGQGWQTVSSIPASAAMPLWRHKVAKWVSKPNSPNFG
                     LLLDVQGLLPPSSTEDGVQMELSFIGGNNLQVGSSRR"
     gene            4823..5269
                     /locus_tag="VGB57_04685"
     CDS             4823..5269
                     /locus_tag="VGB57_04685"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEY0130682.1"
                     /translation="MRKFGLAASAMLCLTGFTPTPGERPFWLGGRWVHEGKGSWSEES
                     WLSRGKMLVGVSMSGSGDVAKSYEFMRIETGKDGRTTFWASPQGRSPVPFRMISLSAA
                     EAVFENPANDYPTRITYRRSDDVLVATISGPGGTNAESWRYRRLRD"
     gene            complement(5266..5535)
                     /locus_tag="VGB57_04690"
     CDS             complement(5266..5535)
                     /locus_tag="VGB57_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEY0130683.1"
                     /translation="MADLHFELVTPERLIRSEAVFMVVVPGTEGDFGVLAGHAPFMST
                     LRNGDIAVYRSGFGDTPERIAVEGGFAEVSERGLTVLAERADVPA"
     gene            complement(5537..6070)
                     /locus_tag="VGB57_04695"
     CDS             complement(5537..6070)
                     /locus_tag="VGB57_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130684.1"
                     /translation="MLRILAAFLLLPLAVAAAAQVREQPIDEIAADLAYGYCPLFLAG
                     QFSLTGPELAQRGFGKTIVRQPHPRVGEMSLVGAKLPDGQIQFGGAAGQACTVIVSAP
                     KSTRGAVLARLRSNMAFMGLDFKPAVSPGPGVASLAGAKIETFRAPVEKQFLYLQHIQ
                     MDDPDGVVVAQLFATDQ"
     gene            complement(6076..>6859)
                     /gene="atpD"
                     /locus_tag="VGB57_04700"
     CDS             complement(6076..>6859)
                     /gene="atpD"
                     /locus_tag="VGB57_04700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0130685.1"
                     /translation="FGQMNEPPGARARVALSGLTQAEYFRDVEGQDVLFFVDNIFRFT
                     QAGSEVSALLGRIPSAVGYQPTLSTDMGALQERITSTNKGSITSVQAIYVPADDLTDP
                     APATSFAHLDATTVLSRAISELGIYPAVDPLDSTSRVLTARTVGQEHYDTARAVQEIL
                     QRYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFHVAEVFTGIPGKFVQVED
                     TVRSFKAVVDGEYDHLPESAFYMVGGIEEAVEKAQRLAAEAA"
BASE COUNT         1084 a         2211 c         2371 g         1193 t
ORIGIN      
        1 aggccggagc cgcccgacgc accaccgccg gaaccgccca ccgacgaacc gcccctgccg
       61 ccgcgtgaga agctcgtgat caggctggcg gacggcaagg aacgaacctt ccagcatatc
      121 agcggcacca ccttttggga ctcgagcggc aaaccgatct cggcccagca gttcgtcgag
      181 cagttgttcg gcaagctgcc cgagctgttc cgtgacgagc acgaactgag gcggatctgg
      241 agtgatcccg acacgcgcaa ggcgctggtg gcgggtctgg cggagcgcgg cttcgccggg
      301 gagcagcttc agcagatcag gcggatgatc gacggcaccc aaagcgatct gttcgacgtg
      361 ctccgctaca tcgcctttac caaagcccct ctcaaacgcg ccgagcgggc gagcacccgc
      421 cgcgacgcga tcttggcgcg atggaacgta cgtcagtcgg agttcctgga ctttgtgttg
      481 tctcaatatg tttccgaggg cgaagaggag ttgggtaccg ataagctccc cgatttgctg
      541 gagctgaagt acggcacccc cgccgacgcc gtccgccgac tgggtagcgt cagcggcatt
      601 cgcgaggcgt tccgaggttt ccaaaaggac ctttacggcg acgacaacta acctcttcct
      661 cgaccgtgca gggggtgcta caagtgtagc aggatcggca cccaatggag cgtccccaga
      721 tgaaagcaac agcaacccgc gcggcggcct tggccgtcct cgctctggct ctcgccggcc
      781 ccgccccggc cgcctccgcc ccgccccagg cccagcagga cgagctctcc actctaatcg
      841 accgcaacct ccgctccggc gggtcctggt tcaccgccga ggagcgcgcc gtgatcgagc
      901 agaagtgcgg ttatgcgccg ggggcgtggg acgggttcga ggcgaatatg tcggacggaa
      961 cctttacctg ccgcgacggc cgcaaggtcg acgatcccga ggtccgggcc gtgctccggg
     1021 ccgtggcgcc gcggatcgag gcccgggttg aggcggtgat ggcccgggcc gacatcgttg
     1081 cggcgatcgc ccgggtcgcc ggggtagctg cggcggaggc gctgcgcgag gtcgaggctc
     1141 ggcaccggcg ctgaggcagg ctcggggggc ttcacgcagg atcgtgcatg attatgcacc
     1201 cgaagaccgg tttcgagggt gccggtggat aaggatgcag gctgccggtt gggtgagggc
     1261 gtgccaccgg tcgaatcccg acggggaatc ggccccagtg gacgcctacg cctcgcgccg
     1321 ctttctttct cggagcgggc tctgtcggcc ggagcgcccc tgaaccgttc ggccgtggcc
     1381 tcttccgcgg acagtagcga ttagtcgcga tgaatggcga ttatcggcac aggaaatggc
     1441 ggaattccgc cgttccttgc catgatgcgg ctattgcagg agcttttcgt aaaccaacct
     1501 gaatttgccc aaacttcgcc gcctcgaaac cctacaaaca cgcctccaga taaggctggt
     1561 cgaagccgaa ctgcttggcc ttctccagcg tgtagggcct cagccccatg ctccggtact
     1621 cgccgatgat cttgccgtcg gcggtctcgt cgagatattc gaacttgaac aattcctggg
     1681 tgacgatgac gtcgccctcc atcccgataa cctcggtgat gttggtggtc cgccgggttc
     1741 cgtcgcgcag gcgcatgacc tggacgatga ggtcgaccga gtccgcgatc tgcctggaga
     1801 tcgcctcctt gggcactttg atgtcgctca tcatcaccat gttctccatt cgcgccaggc
     1861 attcgcgagg ggagttggag tggagggtgg ccatcgagcc gtcatggccg gtgttcatcg
     1921 cggcgagcag gtcgaagcat tccgggccgc ggatctcgcc gaggatgatc cggtccgggc
     1981 gcatgcggag ggcgttgatg acgaggtcgc ggatcgagat cgcgccctgg ccctcgaggt
     2041 tgggcggccg ggtctcgagc ggaagccagt gcggctgctg gagccggagc tcggcggcgt
     2101 cctcgatggt gagaacccgc tcgcccgggt cgatcagctt ggacaaagcg ttgagcatcg
     2161 tcgtcttgcc cgagccggtg ccgcccgaga tgatgatgtt gaagcggctg gcgccggcga
     2221 tcttcaggac cgtcgccatc ttgtccgaca tcgagccgaa attcttcatc atgtcgacgg
     2281 tgatcggctt ctccgagaac ttgcggatcg agatggccgt tccgcgcagg ctcaagggcg
     2341 ggatgatgac gttgacccgg cttccgtcct gaaggcgggc gtcggcgagc ggggtggtct
     2401 ggtcgacccg gcggccgacc ttgttgacga tgcgctgggc gatctggaac agatgctcct
     2461 cgtcgcggaa ctggatctgg gcgatctcga gcttgccctt gcgctcgatg aaggtctggt
     2521 tcggcccgtt gaccatgatg tcggagatgt tcgggtcggc gagcaattcc tccagcggcc
     2581 cgaggccgag aagctcgtcg accagcacct tctcgagcgc gaactgctcg cggcggttca
     2641 ggttgatctt gagctcggcg agcacctcgc cgatgatcgg ccggaattcc tcggccagct
     2701 cgtccttgga gagggtcgcc gccgcttccg ggtccacccg ctcgaggagg cgggggagga
     2761 cctgctcctt gatccggtgg atggaggctt cgaagccctc gttcttgctg ctcggcgctt
     2821 ccgaggaggc ggcctggcgg tcggcgagcc tggacatggc gtcggcgccg cggtcgcccg
     2881 tgggggtgaa gctgtcggtg tccgagtccg agaaactgat cgtgtcgagc ggcgggaact
     2941 ggtcgccgcc ttcgggcgcc accgccggag ccacggcgcc gcccgggccc ttcatcggcc
     3001 tcgcgatgcc gaacgtgggc cgtccgcccc ccgtgccgcc atttcctggt cgccgtccaa
     3061 aagcactcat tgcgctcaga tcctcaccgg tgaagctgtt ccacttggac tggtgaataa
     3121 ggcggaaact ttgacaattg cctaaccgcc cccgatggcc ggccgggtgc ccgcccaaca
     3181 cgcccgaagc gccctgctca tcgctcttgc cggtttcctc agccttgcct tcggcgacgc
     3241 gctggtgaag tcgatggccg ggcagtggcc ggcgccggcg gtcgccgcct tgcgctacag
     3301 cttcggcgcc gtcgggctca cgttgtacgt ggcgcttcgt catggccgcg aaggcttcgt
     3361 catgcctcgg ccgtggcttc acgtcgggcg cggcgccgcg gtcgcgcttg ccaccttgtg
     3421 cttcttcctc ggggtgatgg cgatgccgct cgccgacgcg accgcgatcc agttcaccag
     3481 cccgatcctg acagccctgc tggcccccct ggtgcttgga gaaagaaccc ggccggcgac
     3541 ctgggcggcg accttgctcg ccttcctggg cgttctgatc gtgctccgcc cgaacctgct
     3601 cgagatcggt ccggccgccc tgttcccgat gggcgccgcc ttcgggatga gctggctgat
     3661 gatgttcaac cgcatggccg cgggagacgc cccggtgatg gtgatgcagt tcctggtcgc
     3721 ggtcgtggcg gcgccgctgc tgatcgccgc ggccgctgca ttccacgcat ccggggtgac
     3781 gatcggctgg ccctccgccg tcgtcgtcct gaagtgcctc ggagtcgccg gtttcgcgac
     3841 cgcggggcac gccctgatct tcgccgccgt cgagcgcgcc agcgcgtcga ccgtcgctcc
     3901 gatgacctac gtccagctgc tcgtagccgc cttcctcggc tggctctggt tcggcgacgc
     3961 gcccgacgcc gccaccttcg gcggcgccgc tctcatcatc ggcggcggtc tgctattgtg
     4021 gcgggcgcag cgcgtccgtc atctccgcga ggagccgact tgaagattat tcccgccgat
     4081 gaagctcagc tccatctgga cgccgtcttc ggtgctcgag gggggaagca atccctggac
     4141 gtcgagcaga agcccgaagt tcggcgaatt cggcttcgaa acccacttcg ccaccttgtg
     4201 gcgccaaagc ggcatcgccg cgctcgcagg aatggaagac acagtttgcc agccctggcc
     4261 ggcgagcgac ttggtgaagg aagcgcccag cgcggccgac ccgccgggga cggcggtcac
     4321 catgatgtgg caaacggccg gctgcggaga gcgtatcacc cacacctgtc cgttgcgggc
     4381 gcggaaatgc gcgaaggcgc ccacgccgaa catactgacc atcgactgcg tggcggcgaa
     4441 gcgcttgacc aggtccggga ccccgccttc cggttcgacg gtggcgccgg cgggaagggg
     4501 aggggccacg ttcttctcgg tgacgacggt ctcgcagtag cgcgcggcgc tcgccagcgc
     4561 cgtctccacc ggtggcgagg ccgccggggc tggagccagc agcgcggcga cgagagtggt
     4621 cccgatgttc atctgaggtc cccgcgtggc gagattcgat cattggtgag actaggagcc
     4681 tgcgaccgcg aaggcaaagg cggttcggac cgaaatggcg agaaggaact ggcgctccgc
     4741 tctcattact gccgtcggtc tgctagcatg agcgacgggg cgaacgaggg accttcgccg
     4801 ggcgccgaac tggagaggac gaatgcgcaa attcgggttg gccgcgtcgg ccatgctttg
     4861 cctcacgggc ttcacgccga ctcccggtga acggcctttc tggctgggcg gtcgctgggt
     4921 gcatgagggc aagggttcgt ggtccgagga gagctggctt tcgcgcggca agatgctcgt
     4981 cggagtctcc atgagcggct cgggcgacgt cgccaagagc tacgaattca tgcgcatcga
     5041 gacgggcaag gatggaagaa cgaccttctg ggcctcgccg caggggcgca gtccggtgcc
     5101 gttcaggatg atatcgctgt ccgcggccga agccgttttc gaaaatccgg ccaacgatta
     5161 tcccacgcgc atcacctacc gccgttcgga cgatgtgctg gtcgcgacga tctccgggcc
     5221 gggcggcacc aatgctgagt cctggcgcta ccggcgcctg cgggattaag cggggacgtc
     5281 cgcccgctcg gccagcaccg tcagcccgcg ctcgctcacc tcggcgaagc cgccctcgac
     5341 cgcgatccgc tcgggcgtgt cgccgaagcc gctgcggtag acggcgatgt cgccgttcct
     5401 gagcgtcgac atgaagggcg cgtggccggc gagcactccg aaatcgcctt cggtgccggg
     5461 cacgacgacc atgaagaccg cttcggagcg gatgaggcgt tccggggtga cgagctcgaa
     5521 gtgaaggtct gccatcttat tggtccgttg cgaaaagctg ggcgacgacg accccgtccg
     5581 gatcgtccat ctggatgtgc tggaggtaga ggaactgctt ctcgaccggc gcccggaaag
     5641 tctcgatctt cgccccggca agcgacgcga cgcccgggcc ggggctcacg gccggtttga
     5701 agtcgaggcc catgaaggcc atgttggagc gcagcctggc cagcacggcg ccgcgtgtcg
     5761 acttcggggc gctgacgatc acggtgcagg cctggccggc cgccccgccg aactggatct
     5821 ggccgtccgg cagcttggcc ccgaccaggc tcatctcgcc gactcgggga tgcggctgcc
     5881 tgacgatcgt cttgccgaag ccgcgctgag cgagctccgg gccggtcagc gagaattggc
     5941 cggccaggaa gagcggacaa tagccgtagg cgagatcggc ggcgatctcg tcgatcggct
     6001 gctcgcggac ctgcgccgcc gcggccaccg caagcggcag cagcaaaaag gcggcgagga
     6061 tccgaagcat cgcgcttacg ccgcttcggc ggcgaggcgc tgcgcctttt cgaccgcctc
     6121 ctcgatcccg ccgaccatgt agaaggcgga ctccggaaga tggtcgtact cgccgtcgac
     6181 caccgccttg aacgagcgga cggtgtcctc gacctggacg aacttgcccg ggattccggt
     6241 gaagacctcg gcgacgtgga agggctggga gaggaagcgc tggatcttgc gggcacgcga
     6301 gacggtcagc ttatcctctt ccgaaagctc gtccatgccg aggatggcga tgatgtcctg
     6361 gagcgacttg tagcgctgaa ggatctcctg gacggcacgg gccgtatcgt aatgctcctg
     6421 gccgaccgta cgcgccgtca gaacgcgcga cgtggagtcg agcggatcga ccgccgggta
     6481 gatgccgagc tccgagatgg cgcggctgag caccgtcgtc gcgtcgaggt gggcgaagga
     6541 ggtggccggc gccggatctg tgagatcgtc ggcgggcacg tagatcgcct gcaccgaggt
     6601 gatcgagccc ttgttggtgg aggtaatccg ctcctgcagc gcgcccatgt cggtcgacag
     6661 ggtaggctgg tagcccacgg cggaagggat acggccgagc agcgccgaca cttccgagcc
     6721 cgcctgggtg aagcggaaaa tgttgtcgac gaagaacagc acgtcctggc cttcgacgtc
     6781 gcggaaatat tcggcctggg tgaggccgga caaagcgacg cgggcgcggg cgcccggcgg
     6841 ctcgttcatc tggccgaag
//
LOCUS       DASWNU010000238         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_192059, whole genome shotgun sequence.
ACCESSION   DASWNU010000238 DASWNU010000000
VERSION     DASWNU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192059"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VGB57_04705"
     CDS             <1..1193
                     /locus_tag="VGB57_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0130686.1"
                     /translation="VLALYLLIPIVALSAMPVTQDAAGNYGTELGTTFADDPILGIVE
                     NLGLAHGLTEIMRYYVGVLAAVILLIATNAGLIGVSRLTYSMGQHRQLPEGLRQVHPK
                     YRTPYIAILIFAAVAIVTLLPGQTDFLATMYSFGAMLSFTIAHVALIQLRRKQPDVER
                     TWKPPLNFRAFGVEVPLSAVLGGLGTFLAWIVVMVLNPRTLIVGLGWMALGIALYVLY
                     RRGQRLPLTETVKVVTPEPLGVEEVEYRSILVGFKDDEEFSPEMVATAVKLASKRRRG
                     IHVHSMMTVPTNLPLDAKMPAQEGEAQSKIEEAKLIGGGRVTGHVARVRPGQAGYAVA
                     DEAAEIKAEAIVIGLRYRNGVPLYDKTLQTVLGERPCRVIVVSDPTDKPAAVPGAALM
                     ETTR"
     gene            1190..1381
                     /locus_tag="VGB57_04710"
     CDS             1190..1381
                     /locus_tag="VGB57_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130687.1"
                     /translation="MNGERVYRGAVRAISLAMAAIGLAVLVVTLANGGGPASYGFLIG
                     IAFLAVGAGRLWLASRSGE"
BASE COUNT          221 a          563 c          527 g          243 t
ORIGIN      
        1 ccgtcctcgc cctctacctg ctgatcccga tcgtcgccct ctcggcgatg ccggtcaccc
       61 aggacgccgc cggcaactac gggaccgagc tcggcaccac cttcgccgac gacccgatcc
      121 tcggcatcgt cgagaacctg ggcctcgccc acggcctcac cgaaatcatg cgctactacg
      181 tcggcgtcct cgcggccgtg atcctcctga tcgcgaccaa cgcgggcctg atcggcgtct
      241 cccgcctcac ctactcgatg ggccagcacc gccagcttcc cgagggcctg cgccaagtcc
      301 accccaagta ccgcaccccc tacatcgcga tcctgatctt cgccgcggtc gcgatcgtca
      361 ccctgctgcc gggccagacc gacttcctgg cgacgatgta ctcgttcgga gcgatgctct
      421 cgttcacgat cgcccacgtc gcgctgatcc agcttcggcg caagcaaccc gatgtggagc
      481 ggacctggaa gccgccgctg aacttccgcg ccttcggcgt cgaggtgccg ctcagtgccg
      541 ttctcggcgg gctcggcacc ttcctggcct ggatcgtcgt catggtcctc aatccccgca
      601 ccttgatcgt cgggctcggc tggatggcgc tcgggatcgc tctctacgtc ctctaccgcc
      661 gcggccagcg gctgccgctg acggagaccg tgaaggtggt tacgccggag cccctcgggg
      721 tcgaggaggt cgagtaccga agcatcctcg tcggcttcaa ggacgacgaa gagttctcgc
      781 cggagatggt cgcaaccgcg gtcaagctcg cctccaagcg ccgccggggc atccacgtcc
      841 actcgatgat gacggtgccg acgaacctgc cgctggacgc gaagatgcct gcgcaggaag
      901 gcgaggccca gagcaagatc gaggaggcga agctgatcgg cggcgggcgg gtcaccggcc
      961 acgtggcccg ggtgcggccc gggcaggcgg gctacgccgt cgccgacgag gcggccgaga
     1021 tcaaggcgga ggcgatcgtg atcgggctcc gctaccgcaa cggggtgccg ctctacgaca
     1081 agaccctgca gacggtcctc ggcgagcgcc cctgccgggt gatcgtcgtc tccgacccca
     1141 ccgacaagcc ggcggcggtc cccggcgccg cgctgatgga gacgacgagg tgaacggcga
     1201 gcgcgtctac cgcggcgccg tccgcgccat ctcgctggcg atggcggcga tcgggctggc
     1261 ggtcctggtg gtcaccctcg ccaacggggg aggccccgcc tcgtacgggt tcctgatcgg
     1321 gatcgccttt ctcgccgtgg gcgcgggccg gctgtggctg gcgtcgaggt cgggcgagtg
     1381 agcgcggctg ggggttcgcc gcagagggac ggcaaggagc cggagcggcc ggaggagctg
     1441 cagaccgagc cggtcctgat gcgcggcctc ggcgtgccga tgctcttcgc cgcggcctac
     1501 tcggcggtcg gcttctcgat ctacttcgcc ctcggcgtcg tcgccgacaa gggc
//
LOCUS       DASWNU010000239         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_424303, whole genome shotgun sequence.
ACCESSION   DASWNU010000239 DASWNU010000000
VERSION     DASWNU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424303"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1930)
                     /gene="cysC"
                     /locus_tag="VGB57_04715"
     CDS             complement(128..1930)
                     /gene="cysC"
                     /locus_tag="VGB57_04715"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY0130688.1"
                     /translation="MDKTLLRLITCGSVDDGKSTLLGRLLFETGSAFDDELALCGDDL
                     SRLVDGLEAEREQGITIDVAYRFFATKRRKYIVADAPGHEQYTRNLVTGASTAELALV
                     LVDARKGILTQTRRHSYLCRLLGVSRFVLLVNKMDSVGFDRGAFDRIVADYAALGESF
                     TAIPVSALSGDNVAVRSPAMPWYDGPSLLEHLEGVEPEAEGDGGPFAMPVQWVSRHGR
                     RGFAGMVAGGSVRRGDRVRVMPAGTRATVERIAVGHAELDEAVKGQSVMLSFAEEVDC
                     SRGDVVASADYPPEAADQFEATIVWMAEEELVAGRQYGLKLAAQSALATVHTPKYEID
                     VNTFDQVPAQGLQLYGIGVANLWTDRPLVFEAYEDNRDLGGFILIDRVTNDTVGAGLI
                     RHALRRSQNLHLQALDVTPGARAAMKGQKPRLLWFTGLSGAGKSTIANLVEKKLHLAG
                     RHTFLLDGDNVRLGLNRDLGFSDADRAENIRRVGETARLMLDAGLIVLAAFISPFRAE
                     RRMVREMLGEGEFVEVHVDVPLAVAEERDPKGLYAKARDGRIPNFTGIGSPYEPPEAP
                     DIRIDAAALSPEEAAEAVVRYLLAPPRNGEGDQP"
     gene            complement(2022..>2828)
                     /gene="cysD"
                     /locus_tag="VGB57_04720"
     CDS             complement(2022..>2828)
                     /gene="cysD"
                     /locus_tag="VGB57_04720"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY0130689.1"
                     /translation="SAGKDSSVLLHLARLAFAPAKPPFPLLHVDTMWKFRAMYELRDR
                     AAAEAGMELIVHRNPEAEERGINPLDHGSALHTQMWKTDGLKQALDRHGFDAALGGAR
                     RDEERVRAKERVFSFRSAGHRWDPKRQRPELWRLYNARKAKGESFRIFPLSNWTELDV
                     WQYVEAERIEVSSLYFAEPRPTLERDGTLLMVDDERLKPGPGEEVRERWVRFRTLGCY
                     PLSGAIESRATDVHGLIEEMRAGRQSERQGRLIDRDGTASMERKKREGYF"
BASE COUNT          423 a         1001 c          972 g          432 t
ORIGIN      
        1 gggcggcagc ctggtgttcc ggcggggtta ggatcgtcct cgccatcgaa agatggggag
       61 ggggaccagg cgcagcctgg cggaggggta actggcagaa gcctgcgcga gctacccctc
      121 caccagccta cggctggtcc ccctccccgt tccggggagg agcgaggagg tagcgcacca
      181 ccgcctccgc cgcctcctcg gggctcagcg ccgcagcgtc gatccggata tccggcgcct
      241 cgggcggctc gtaaggcgag ccgatgccgg tgaaattggg gatccgcccg tccctcgcct
      301 tggcgtagag gcccttgggg tcgcgctcct ccgctaccgc caaagggacg tcgacatgca
      361 cctcgacgaa ctccccttcg ccgagcatct cgcgcaccat ccgccgctcc gcccggaacg
      421 ggctgatgaa ggcggcgagc acgatcaggc cggcgtcgag cataagccgc gccgtctccc
      481 cgacccggcg gatattctcc gcccggtccg cgtcggagaa gccgaggtcg cggttgaggc
      541 ccaggcggac attgtcgccg tcgagcagga aggtgtgccg cccggcgagg tgaagcttct
      601 tctcgacgag gttggcgatg gtcgacttgc ccgccccgga aaggccggtg aaccagagca
      661 ggcgcggctt ctgccccttc atcgccgccc gcgccccggg ggtcacgtcg agggcctgga
      721 gatggaggtt ctgcgagcgc cgcagggcgt ggcggatgag gccggcgccg accgtgtcgt
      781 tggtcacccg gtcgatgagg atgaagccgc cgaggtcgcg attgtcctca taggcctcga
      841 acaccaaagg ccggtccgtc cacaggttgg cgaccccgat cccgtacagc tgaagcccct
      901 gcgccggcac ctgatcgaac gtgttgacgt cgatctcgta tttgggcgtg tggaccgtcg
      961 ccagcgcgct ctgcgccgcg agcttcaggc catattgccg cccggcgacc agctcctcct
     1021 ccgccatcca gacgatcgtc gcctcgaact ggtcggcggc ctcgggcgga tagtcggccg
     1081 acgcgacgac gtcgccgcgc gagcaatcca cctcctcggc gaaggacagc atcaccgact
     1141 ggcccttcac ggcctcgtcg agctcggcat ggcccaccgc gatccgctcg acggtcgccc
     1201 gggtgccggc gggcatcacc cgcacccggt cgccccgcct caccgagccg ccggccacca
     1261 tcccggcgaa gccgcgccgg ccgtggcggc tcacccattg caccggcatc gcgaaaggcc
     1321 cgccgtcgcc ctcggcctcc ggctcgactc cctccagatg ctcgagcagc gagggcccgt
     1381 cgtaccaggg cattgccggc gaccggaccg cgacattgtc gccgctcagc gcggagaccg
     1441 ggatggcggt gaagctctcg cccagggccg cataatcggc gacgatccgg tcgaaggcgc
     1501 cccggtcgaa gccgaccgag tccatcttgt tcaccagcag cacgaaacgg gacacgccga
     1561 gcaggcggca cagataggaa tggcgtctcg tctgggtcag gatccccttg cgagcgtcga
     1621 cgaggaccag ggccagctcc gcggtcgagg cgccggtcac gaggttgcgc gtatattgct
     1681 catgccccgg agcgtcggcg acgatatact tgcgccgctt ggtcgcgaag aagcgatagg
     1741 cgacgtcgat cgtgatcccc tgctcgcgct cggcctcgag accgtcgacg aggcgcgaga
     1801 ggtcgtcgcc gcaaagtgcg agctcgtcgt cgaacgcgga gccggtctcg aagagaagcc
     1861 ggccgagcag ggtcgacttg ccgtcgtcga cggagccgca ggtgatgagg cggaggaggg
     1921 tcttgtccat cacgtgatcc cggcgaaagc cgggacctcg gggagacccg ccgtgccttc
     1981 tcgtcctggg atcccggctt ccgccgggat gacggtagcc atcagaaata cccctcgcgc
     2041 ttcttgcgct ccatcgacgc cgttccgtcg cggtcgatca gccggccctg ccgctccgac
     2101 tggcggccgg cgcgcatctc ctcgatcagc ccgtgaacgt cggtcgcccg gctctcgatc
     2161 gccccggaga gcgggtagca gccgagcgtg cggaagcgca cccagcgctc ccgcacttcc
     2221 tcccccggcc ccggcttcag ccgctcgtcg tcgaccatca gcagggtgcc gtcgcgctcc
     2281 agcgtcggcc gcggctcggc gaaatagagg gaggagacct cgatccgctc ggcctcgaca
     2341 tattgccaga cgtcgagctc ggtccagttg gagagcggga agatccggaa gctctccccc
     2401 ttcgccttgc gggcgttgta gagccgccag agctcgggcc gctggcgctt cgggtcccag
     2461 cgatggccgg cggagcggaa ggagaagacc cgctccttcg ccctcaccct ctcctcgtcg
     2521 cgcctggccc cgccgagggc ggcgtcgaag ccgtggcggt cgagcgcctg cttcaggccg
     2581 tccgtcttcc acatctgggt gtggagcgcc gagccgtggt cgagcgggtt gatcccccgc
     2641 tcctcggcct ccgggttgcg gtggacgatg agctccatcc ctgcttcggc ggcggcgcgg
     2701 tcgcgaagct cgtacattgc cctgaacttc cacatcgtgt cgacgtggag gagggggaag
     2761 ggcggtttcg ccggagcgaa ggcgagcctg gcgaggtgga gcaggaccga gctgtccttg
     2821 ccggccga
//
LOCUS       DASWNU010000240         7678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_449353, whole genome shotgun sequence.
ACCESSION   DASWNU010000240 DASWNU010000000
VERSION     DASWNU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449353"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2789)
                     /locus_tag="VGB57_04725"
     CDS             complement(<1..2789)
                     /locus_tag="VGB57_04725"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130690.1"
                     /translation="MPRFGQNNFTSRLFADGEQSSAFGYDLHSRGLFASQAAARQIGL
                     DVGAAGGSKLTTVNVPGNYQPVESAWYVPGPTALVDGYTAAPNTTGTLVVDDPNHVLA
                     ASDTPGDNDWFKVTLTAGQTYQFGQYARAGGVPGVEGNGIPLADAYLEIYDSAGNLLS
                     IADGGGPNTPSGLDALMTFEAPYSGTYYVNATSYDNTGDGVGDFVGDYEIFARTATGT
                     VYTTHYDISSPLHSIDWGGVLVNREHVSVRNPDGMEGPRPTGEPAANPATVEGNALGY
                     PGKNVIYIYFAGEGDVYANVNSTIPAQVTAQNPYQYEIQALLTAAAEFSKVADVVYVS
                     NYTLNAATGLYERDPNIQYKPGLLGAEPGMKYATGVDGDGFDADFFYWSYPGTPGPGI
                     SLLGSMNPPDNGDEGVAQFNSADERWNAQMLQKGGFSFVTLIHEFGHGMGLAHPHDTG
                     GGSSVMQGVVTSGVPGDDGGAGAYDLNQGVNTMMSYNDGWEKSPYGQAETNNEGYGWL
                     GSLMALDIAVIQDKYGVNEDWATGNDTYVLKDVNEWGVYIDSATGQPAVHDATDQLTA
                     RDGYYVGESTYYSSIWDAGGVDQITYSGARDTVIDLRPATLRYEYGGAGWMSYATGIY
                     GGFTVANGVTIENATSGSGNDRLTGNAADNRLEGNGGHDTLNLWFGGGNDTALGGAGN
                     DNIFFGNTLNSADIVRGGDGTDTLVLQGAYGALTLTANVTEIEGISLLGGSNTALGEP
                     GTNRFDYSITTIDANFAAGLQVRVNGAALLADEDFTFNGSAETDAQFVVYGGKGRDTL
                     TGGQGNDIFFYAEERFASGDTVNGGAGYDGMFLRGNYTIDFNAPGYTGLFTNIENLTL
                     TSATDERYARGGGTEFDYNLVLSDAIVNPGYQLTVSGAVLTASETMVFDASAETDGTL
                     RLFGGRASDTLKGGAL"
     gene            3238..4341
                     /locus_tag="VGB57_04730"
     CDS             3238..4341
                     /locus_tag="VGB57_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130691.1"
                     /translation="MKLACLALLSPFALVGCWPTTEMRDGSDRVRLVEIVRQFCAAER
                     SADPGDTHPLLVKSLREMLDDGASVRPHLTSADPKAGCEPGRTWYLGGSRMFAEVRLG
                     DRSDRLDLWRGEWPRIGDVIYGRKRQVGGRRFKSLRAELIAEAGGQSAAAAPPPPPPP
                     DMECLQPDYRFAFVGTDTIIYRQGAAVRVTPMVDRSPAGTAELPLRCTSGWSVIGPAT
                     LSADRTSITIAPDAPAGATITIGFRHEGKAVEKRLKVVAKDEVVLTGRYTQRSLEGCS
                     ANEPVRELEFRPENRFSVTFLPFETYQDYWGTYSFDPATRRLRLTVEGGNFIPSNLDP
                     EGEAELADGRLRLTGLFLGSRHEAPQSNCTYVF"
     gene            4476..5258
                     /locus_tag="VGB57_04735"
     CDS             4476..5258
                     /locus_tag="VGB57_04735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0130692.1"
                     /translation="MLKPLGIRTRIREEWRSLKDLGFAARSAFRKDRKGAAYQAAFDE
                     PEPLVTVCIATFNRGRLLTERSIPSILAQTYRNLELIVIGDGCTDDTAERVAAIDDPR
                     LRFENFTERGRYPEDPHHRWMVAGTGATNRALSIARGSFVTHLDDDDEHDPSRIEKLL
                     ALIRRERADLVYHPFHYEMPDGTWRTNSADRFGIGKVTTSSMFYHRRLAEIPWDVEAW
                     KYLEPGDWNRLRKLRFLGVRSVRHPDPLLSHYRERSQEAATA"
     gene            complement(5335..5410)
                     /locus_tag="VGB57_04740"
     tRNA            complement(5335..5410)
                     /locus_tag="VGB57_04740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5375..5377),aa:Thr,seq:cgt)
     gene            5637..6890
                     /locus_tag="VGB57_04745"
     CDS             5637..6890
                     /locus_tag="VGB57_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130693.1"
                     /translation="MDSLRAFDEQSDYGVAEDDGGIEVPPEIGLDERRMHVRAYNYWV
                     SLLDGRAYPSIKALDPEALGDFGPNSVLLDFSGSGDDPKIAWLGDALRGECDLIGDVR
                     RISEVPKRSLLSRLTDHYMQIIANKAPIGFEAEFVGQRGKNTMYRGILMPFSSTGETI
                     DFIYGVINWKEIADSATASAIAHQASTALAAAAQPGASPVWADGPNAEPLEMEAPEAA
                     WDEPELDLDAGLADRLGAARDGADAARGADQRSRASLYRALGLAYDFALAAEADWEGY
                     SELLEDAGLRAQARAPMTPVVKLVFGTDYDKGRLTEFAAALSWARRHDLPAGALRERL
                     ESEGLKALVQAERRARRPEPKPDTGEAAREALRAVRPLGHVDIAGEEEFVLLIARRDE
                     VGGLAVLAPVADQAMLERAIRKAAA"
     gene            complement(7067..7450)
                     /locus_tag="VGB57_04750"
     CDS             complement(7067..7450)
                     /locus_tag="VGB57_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130694.1"
                     /translation="MMLLLALLLGAAPDAAALAKIGITGRTGAPDAATAQAALTEAGG
                     FAAGHALKCGEVGGAEASLMPKGWVPADPNFRLGPKGARYERWEIKLCGKNVPFLVVF
                     WKHKGRPDFQVAHPFPADPAKRVRP"
     gene            complement(7447..>7678)
                     /locus_tag="VGB57_04755"
     CDS             complement(7447..>7678)
                     /locus_tag="VGB57_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HEY0130695.1"
                     /translation="DKTSATTNVRQLADKESAVNLNMGTLVGSYESVARMLDEASTVP
                     NTGGVLLTFDDFIEGTENFGTRIQPLMKCRRG"
BASE COUNT         1280 a         2523 c         2556 g         1319 t
ORIGIN      
        1 agtgccccgc ccttcaaggt atccgaggcc ctgccgccga acagccgcaa ggtgccgtcc
       61 gtctccgcgc tggcgtcgaa caccatcgtc tcgctcgccg tcagcaccgc gcccgacacc
      121 gtcagctggt accccggatt cacgatcgcg tcggaaagga cgaggttgta gtcgaactcg
      181 gtgccgccgc cgcgcgcgta gcgctcgtcg gtggccgagg tcagggtcag attctcgatg
      241 ttggtgaaca ggcccgtata gccaggcgcg ttgaagtcga tcgtgtaatt gccgcgcagg
      301 aacatgccgt catagccggc gccgccgttg accgtgtctc cgctcgcgaa ccgctcctcg
      361 gcatagaaga agatgtcgtt gccctggccg ccggtcaggg tatccctgcc cttgccgccg
      421 tagacgacga actgggcgtc ggtctccgcc gagccgttga acgtgaagtc ctcgtccgcg
      481 agcaacgccg cgccgttgac ccgcacctgc aaccccgccg cgaagttggc gtcgatggtg
      541 gtgatcgaat aatcgaagcg gttggtgccg ggctcgccga gagcggtgtt cgagccgccg
      601 agcagcgaaa tcccttcgat ctccgtgacg ttcgcggtga gcgtcagggc cccgtaggcg
      661 ccctgcagca ccagggtatc ggtgccatcg ccgccgcgga cgatgtcggc cgagttcaag
      721 gtattgccga agaagatgtt gtcgttgccg gcgccgccga gcgcggtgtc gttgcctccg
      781 ccgaaccaca gattgagggt atcgtgaccg ccattgcctt cgagacggtt gtccgcggcg
      841 ttgccggtca gcctgtcgtt gccgctgccg gaggtcgcgt tctcgatggt gactccgttg
      901 gcgacggtga agccgccgta gatgccggtg gcgtaggaca tccatccggc gccgccatat
      961 tcatagcgga gagtcgcggg gcgcagatcg ataacggtgt ccctggcgcc gctataggtg
     1021 atctgatcga cccctccggc atcccagatc gacgaataat aggtgctttc gcctacataa
     1081 tagccgtcgc gggccgtcaa ttgatcggtg gcatcatgca cggccggctg cccggtcgcg
     1141 gaatcgatat agactcccca ctcgttcacg tccttcagga cgtaggtgtc gttgccggtg
     1201 gcccaatcct cgttgacgcc atatttgtcc tggatgacgg cgatgtcgag cgccatcagg
     1261 ctgccgagcc agccatagcc ctcgttgttg gtctcggcct ggccgtaggg cgacttttcc
     1321 cagccgtcat tgtaggacat catcgtgttg acgccctgat tgagatcgta cgctccggcc
     1381 ccgccgtcgt cgccggggac gccgctggtc accacgccct gcatgacgct cgagccgccg
     1441 ccggtgtcgt gcgggtgagc gaggcccatg ccgtggccga attcgtggat cagagtgacg
     1501 aaggagaagc cgcccttctg aagcatctgg gcgttccagc gctcgtcggc ggaattgaac
     1561 tgggcgacgc cctcgtcgcc attatcgggc gggttcatcg acccgagcag tgaaatgccc
     1621 ggccccggcg taccgggata ggaccagtag aagaaatcgg catcgaagcc gtcaccatcc
     1681 actccggtgg catatttcat gccgggctcg gcccccaaca ggccgggctt gtactggata
     1741 ttcgggtcac gctcgtagag gccggtcgcc gcgttcaatg tataattgga gacatagacg
     1801 acgtcggcga ccttggagaa ttcggccgcc gcggtcagca gggcctggat ttcgtactga
     1861 tacggattct gggccgtgac ctgggccggg atggtcgaat tgacattcgc atagacatcg
     1921 ccctcgccgg cgaaatagat gtagatgacg ttcttgcccg ggtagccgag ggcgttgccc
     1981 tcgacggtcg ccgggttcgc cgccggttcc ccggtcggtc gcgggccttc catgccgtcc
     2041 ggattgcgca ccgagacgtg ctcgcggttg accaggactc cgccccagtc gatcgagtgg
     2101 agcgggctcg aaatgtcata atgcgtggta tagacggttc cggtcgcggt cctggcgaaa
     2161 atctcgtaat cgccgacgaa atccccgacc ccgtcgccgg tattgtcgta ggacgtggcg
     2221 ttgacgtagt aggtacccga ataaggtgcc tcgaacgtca tgagcgcatc gagccccgac
     2281 ggcgtgttcg gcccgccgcc atccgcgatc gagagcagat tgccggcgga atcgtagatt
     2341 tcgagatagg catcggcgag cgggatgccg ttgccttcca cgcctggaac gccgccggca
     2401 cgggcatatt gaccgaactg gtaggtttgg ccggccgtca gcgtgacctt gaaccagtca
     2461 ttgtcgccgg gcgtgtcgct tgccgccagg acgtggttgg gatcgtcgac caccagcgtc
     2521 ccggtggtgt tcggggcggc cgtatagccg tcgacgaggg cggtcgggcc ggggacgtac
     2581 caggccgatt cgaccggctg gtagttgccg ggaacgttga cggtggtcag cttcgacccg
     2641 ccggcggcgc cgacgtccag cccgatctgc cgggccgcgg cctgcgaggc gaagagcccg
     2701 cgcgaatgaa ggtcgtagcc gaacgcgctc gactgctcgc cgtccgcgaa caggcgcgac
     2761 gtgaagttat tctgcccaaa tctcggcatc ccgaattctc cctgatcaat tatggcgtga
     2821 acgcggcttc gaggacgccg cccggacctc gcggatcggg ggcgtcatca agcctgaggc
     2881 tcgtatgttt tggaatggac ctggcggaga cggggacggc caatggcacg tgcggaacgc
     2941 ctgccggagc agagaagcaa cgcgggttgc aacgcgcgga cgaatcccaa ttttcgcctt
     3001 tcgaacagct aaggatagta aaggagctgt taacgctgct gaaacccgcg ccgggcggtt
     3061 cggttccggt acgaagggaa cctgctgaaa ctacgtagat aatcgtgagc gggagtcccc
     3121 cggcgagaca gagcggaggt ccatccgccg atgcgccgcg gcggcttgat cccggtggcg
     3181 aatgtcgtca gcctgcggtc gcaatcctgc ccgttcgccg ctgccggagc gtgaccgatg
     3241 aagctcgcct gcctcgcctt gctgtcccct tttgccctgg tcggctgctg gccgacgacc
     3301 gagatgcggg acggcagcga ccgggttcgg ctcgtcgaga tcgtccgcca gttctgcgcg
     3361 gcggagcgaa gcgcggaccc cggcgacact catccgctcc tcgtcaagtc gcttcgggaa
     3421 atgctcgacg acggcgcgtc cgtccggccg catctgacgt ccgccgaccc gaaggccgga
     3481 tgcgagccag gacgcacctg gtatctgggc gggtcgcgga tgttcgcgga ggtgcggctg
     3541 ggggatcgaa gcgaccggct cgatctttgg cgcggggaat ggccccggat cggggacgtc
     3601 atctacggac gcaagcgcca ggtgggcggg cgcaggttca agagcctcag ggcggagctg
     3661 atcgccgagg cgggcgggca atccgccgcc gccgccccgc cgccgccgcc gccgccggac
     3721 atggaatgcc ttcagcccga ctatcgtttc gccttcgtcg gtaccgacac gatcatctac
     3781 cgccaggggg cggccgtgcg ggtcacgccg atggtcgacc ggtcgccggc gggcaccgcg
     3841 gagctgccct tgaggtgcac gagcggctgg tcggtgatcg gtccggcgac cttgagcgcc
     3901 gaccgcacct cgatcacgat cgcaccggac gccccggccg gagcgacgat cacgatcggc
     3961 ttccgccacg aaggcaaggc ggtcgagaag cggctcaagg tggtcgcgaa ggacgaggtc
     4021 gtcctgaccg gtcgctacac ccagcgcagc ctcgaaggct gctcggcgaa cgagccggtc
     4081 cgcgagctcg agttcaggcc ggagaaccgc ttctccgtca ccttcctgcc gttcgagacc
     4141 taccaggatt attggggcac ctacagcttc gatcccgcca ccaggcggct ccggctgacc
     4201 gtcgagggtg gcaatttcat cccgtccaac ctcgatcccg agggggaggc cgagctggcg
     4261 gacggaaggc tgcgcctcac cggccttttc ctcggcagcc gccacgaagc gccgcaatcg
     4321 aactgcacct acgtcttttg aagagagccg ttccggccgg agtcgacgcc gtgcgagggc
     4381 cgatcggaga tcgatggctt ttcgggcggc cgtcgatgcg ataacgggca gcgccttcat
     4441 cctggccgca accgtccgaa agatctccag cccccatgct caagccgctc ggcatacgaa
     4501 cccgaattcg cgaggaatgg aggtcgctca aggatcttgg gttcgccgcc cgatccgcgt
     4561 tccgcaagga tcgcaagggc gcggcctacc aggcggcctt cgacgagccc gaacccctgg
     4621 tcacggtctg catcgccacg ttcaaccggg gcaggcttct gaccgagcgg tccattccct
     4681 cgatcctcgc ccagacctat cgtaacctgg agctgatcgt catcggcgac ggctgcaccg
     4741 acgatacggc ggagcgggtg gccgcgatcg acgatccgcg cctgcggttc gagaatttca
     4801 cggagcgggg gcgctatccc gaagatcccc atcaccgctg gatggtggcg ggcacggggg
     4861 cgacgaatcg cgcgctgtcg attgcgcgcg gctcgttcgt cacgcatctc gacgacgacg
     4921 acgagcacga tccctcgagg atcgagaaat tgctggcgct cataagaagg gagcgcgccg
     4981 acctcgtcta tcatccgttc cactacgaga tgccggacgg cacctggcgc accaattcgg
     5041 ccgatcgctt cgggatcggc aaggtcacca cctcttccat gttctatcat cgtcggctgg
     5101 ccgagatccc ctgggacgtc gaggcctgga aataccttga gccgggggac tggaaccgcc
     5161 tgaggaaatt acgctttctg ggtgtcagaa gcgttcgcca tcccgatccc ttgctgtccc
     5221 actatcgcga gaggagccag gaagcggcga cggcatagaa gccgccgcag ccggcgccgc
     5281 acgccccggc ctggcgggat ctcgccatcg gcggagccgg ataatatttc caggtggtgc
     5341 cgcttgggtg actcgaacac ccgaccccat cattacgaat gatgtgctct accagctgag
     5401 ctaaagcggc gctccttggg ggagggcgcg cgtctatcag cggcggcggg cgatgacaag
     5461 tgacgggtcg acgtcatccc ggcgaaagcc gggacctcag gacgagaagt ctctgcgggc
     5521 ctctacggga ccccggcctt cgccggggtg acggctctgc ggccgtcaaa ccttaacggt
     5581 ttttcaacca cgtccggtgc aaagctccac tcactgggga gcacaaggac cgcctgatgg
     5641 acagcctgcg ggcatttgac gagcagtcgg actacggcgt cgcggaggat gacggcggca
     5701 tcgaggtgcc gccggagatc ggcctcgacg agcggcgaat gcacgttcga gcctataatt
     5761 actgggtctc gctgctcgac gggcgcgcct atccgtcgat caaggcgctg gatccggagg
     5821 cgctcggcga cttcggcccg aacagcgtcc tgctcgattt ctccgggagc ggcgacgatc
     5881 cgaagatcgc ctggctgggc gatgccctgc gcggggaatg cgacctgatc ggcgacgtcc
     5941 gccggatcag cgaggtgccc aagcgctcct tgctctctcg cctcaccgat cattatatgc
     6001 agatcatcgc caacaaggcg ccgatcggct tcgaggccga gttcgtcgga cagcgcggca
     6061 agaacaccat gtaccggggc atattgatgc ccttctcctc gaccggcgag acgatcgact
     6121 tcatctacgg ggtgatcaac tggaaggaga tcgccgacag cgcgaccgct tccgccatcg
     6181 cccatcaggc ttccaccgcg ctcgccgcgg cggcgcagcc gggcgcgagc ccggtctggg
     6241 cggacggccc caatgccgag ccgctcgaga tggaggcgcc ggaggcggcc tgggacgagc
     6301 ccgagctcga tctcgacgcc ggcctcgccg atcgtctcgg tgccgcccgt gacggcgccg
     6361 acgccgcccg cggcgccgac cagcgcagcc gcgcctcgct ctaccgggcg ctcggcctcg
     6421 cctacgactt cgctttggcc gccgaggcgg attgggaggg ctattcggag ctgctcgagg
     6481 atgccgggct cagggcccag gcgcgggcgc cgatgactcc ggtcgtcaag ctcgtcttcg
     6541 gaacggatta cgacaaaggg cgcctcacag aattcgccgc cgccctcagc tgggcgcgcc
     6601 gtcacgacct tcccgcgggc gccctgcgcg aacggctgga gagcgagggc ctcaaggctc
     6661 tcgtccaggc cgagcgccgg gcccgccggc ccgagccgaa gccggacacc ggcgaggcgg
     6721 cgcgcgaggc gctccgcgcc gtccgtccgc tcggccatgt cgacattgcc ggcgaggagg
     6781 agttcgtcct cctcatcgcc cgccgcgacg aagtgggcgg gctcgccgtc ctcgccccgg
     6841 tcgccgacca agcgatgctg gagcgcgcga tccggaaagc ggcggcctga tttgcctccc
     6901 catcgcaaga tggggagggg gaccatgcga agcatggtgg aggggtagag ggcgcaggcc
     6961 ctcgccttct agcaacatat ccgcagcttc agagccatct acccctccac cacacttcgt
     7021 gtggtccccc tccccgccgc tttgcggcag ggaggcaaga attggcctaa ggcctgaccc
     7081 gcttggcggg atccgccgga aacggatggg cgacctggaa atcggggcgg cccttgtgct
     7141 tccagaagac gaccaggaag ggcacgttct taccgcagag cttgatttcc cagcgctcgt
     7201 aacgcgcgcc cttcggtccg aggcggaaat tgggatcggc cgggacccag cctttgggca
     7261 tcagcgacgc ctccgcgccg cccacttcgc cgcatttcag cgcgtgcccg gcggcgaacc
     7321 ccccggcttc ggtcaaggcg gcctgggcgg tcgccgcatc cggagccccg gtccggccgg
     7381 tgatcccgat cttcgcgagg gccgccgcat cgggagcggc accgagcaac agggccagca
     7441 acaacatcat cccctccggc acttcatcag cggttgaatc cgggtgccga aattctccgt
     7501 cccttcgatg aagtcgtcga acgtcagcag cactccgcca gtgttcggca ccgtcgaggc
     7561 ttcgtcaagc atcctcgcga cgctctcgta ggagccgacg agggtgccca tgttgagatt
     7621 gaccgcgctc tccttgtcgg cgagctggcg gacgttggtg gtggcgctgg tcttgtcg
//
LOCUS       DASWNU010000241         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_294071, whole genome shotgun sequence.
ACCESSION   DASWNU010000241 DASWNU010000000
VERSION     DASWNU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294071"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VGB57_04760"
     CDS             <1..297
                     /locus_tag="VGB57_04760"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY0130696.1"
                     /translation="GDVFLENERQMSAWNFEIADTDMLFDQFRKAAGECERCLERALP
                     IPAYEQAIKASHIFNTLQARGVISVAERQAYIGRVRDLAKGACQSWMEKNGWSE"
     gene            478..>1912
                     /gene="glyS"
                     /locus_tag="VGB57_04765"
     CDS             478..>1912
                     /gene="glyS"
                     /locus_tag="VGB57_04765"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY0130697.1"
                     /translation="MPDFLLELLSEEIPARMQGKAQQDLARLFSEQLSSAGLKAEAVE
                     TYATPRRLVLIARGLPAETVAVREETKGPRASAPPQALEGFLRKTGMTREQLEEREGI
                     YYAIVDKPGRATSAVLAEAIPAIVRAFPWPKSMRWGAASSSTESPRWVRPLQGIVALL
                     GEEIVDFEIAGVRSGAATVGHRFHHPGIITIGGAHDYVEKLQACRVVVDPAERRRIVA
                     EGATAAAAAAGLTLVEDEGLIAENAGLTEWPVPLLGSFDPAFLQVPREVIQLTMRTNQ
                     KYFACVDGEGALAPNFVCTANIDAADGGASIVAGNERVLSARLSDAKFFWEQDLKVSL
                     EDQAGKLRDIVFHEKLGTVADKVERVAKLARWLVETGVINPSPWRGEGQVREANQGEG
                     ALSSKGSSTAEPPHPDPLPAGEREDVATLATRAARLCKADLVTQMVGEFPELQGLIGG
                     HLARAQGEDPQVADAVRDHYKPVGQGDE"
BASE COUNT          340 a          686 c          600 g          286 t
ORIGIN      
        1 ggcgacgtct tcctcgagaa cgaacgccag atgtccgcct ggaatttcga gatcgccgac
       61 accgacatgc tgttcgacca attccgcaag gccgccggcg aatgcgaacg ctgcctcgag
      121 cgcgccctcc ccatccccgc ctatgagcag gcgataaagg cgagccacat cttcaacacc
      181 cttcaggcga ggggcgtcat ctccgtcgcc gaacgccagg cctatatcgg aagggtccgc
      241 gacctcgcca aaggcgcctg ccagtcctgg atggaaaaga acgggtggtc ggagtgagca
      301 ttgtttcacc caaagcacca aaggaccaaa gagtcctgcg caagccggcg cgaagcgccc
      361 ctcgattcgg gctgtggagg aaatatggag cggcttcgcc gctcgggcgc ctttggtcct
      421 ttggtgcttc gggtgaaaca gcggccctcg tgaagcccgc gggagcgcga ggctgacatg
      481 cctgacttcc tcctcgagct tctctcggaa gagattcccg cccggatgca aggcaaggcg
      541 cagcaggatc ttgcgcgcct cttttcagag cagctctcaa gtgcggggct gaaggcggag
      601 gcggtcgaga cctacgccac gccccgtcgc ctcgtcctca tcgcccgcgg cctgcctgcc
      661 gagaccgtcg ccgtccgcga ggagaccaag gggccgcgcg cctccgcccc gccgcaggcg
      721 cttgaaggct tccttcgcaa gaccggcatg acccgcgagc agctggagga acgggaaggc
      781 atctattacg ccatcgtcga caaacccggc cgggccacct ccgccgtact cgccgaagcc
      841 attcccgcca tcgtccgagc cttcccctgg cccaagtcga tgcgctgggg cgccgcctcg
      901 tcctcgaccg aaagtccacg ttgggtccgc ccgcttcagg gcatcgtcgc gctgctcggc
      961 gaagagatcg tcgacttcga gatcgccgga gtgcgcagcg gcgccgcgac ggtcggccac
     1021 cgcttccatc atcccggaat catcaccatc ggcggcgccc acgattatgt cgagaagctc
     1081 caggcctgcc gggtcgtcgt cgaccccgcc gagcgccgcc ggatcgtcgc ggaaggcgct
     1141 accgccgcgg cggccgccgc cggcctgacc ctggtcgagg acgaaggcct gatcgccgag
     1201 aacgccggcc tgaccgaatg gccggtgccc ctgctgggaa gcttcgaccc cgccttcctc
     1261 caggtcccgc gcgaggtgat ccagctcacc atgcgcacca accagaaata tttcgcctgc
     1321 gtggacgggg agggcgctct tgctcccaat ttcgtctgca ccgccaacat cgacgccgcc
     1381 gacggcggcg cctcgatcgt cgccggcaac gaacgcgtcc tctccgcccg cctctccgac
     1441 gccaagttct tctgggagca ggacctcaag gtctcgctcg aggatcaggc cgggaagctt
     1501 cgggacatcg ttttccacga gaagctcggc accgtcgccg acaaggtcga gcgggtcgcc
     1561 aagctggcgc ggtggctggt ggaaaccggc gtcataaacc cctctccctg gaggggagag
     1621 ggacaggttc gcgaagcgaa ccagggtgaa ggggctctga gttcgaaagg atcctcaacc
     1681 gccgagcccc ctcaccccga ccctctcccg gcaggggaga gggaggacgt ggccaccctc
     1741 gcgacacgcg ccgcccgcct ctgcaaagcc gatctcgtca cccagatggt cggcgaattc
     1801 cccgagctcc agggcctcat cggcggccac ctcgcccggg cccagggcga agacccacag
     1861 gtcgccgacg ccgtccgcga ccattacaag ccggtagggc agggcgacga gg
//
LOCUS       DASWNU010000242         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_138160, whole genome shotgun sequence.
ACCESSION   DASWNU010000242 DASWNU010000000
VERSION     DASWNU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138160"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VGB57_04770"
     CDS             complement(<1..843)
                     /locus_tag="VGB57_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY0130698.1"
                     /translation="MSVSVEPTPLHEPLAGGTRGATVAVEPLIAGHVEWSRDMMVSPG
                     GRLLTMKLLRSILTGRNAVAVPVPAFLIRHPSAGAILVDTGLHPSVASDARENFGSLG
                     TRFGHPSLEPGEDVPAQLRKRGLEPSEVPIVVMTHLHLDHSSAISEFPNSTFVVSEAE
                     WKAAAQGPSPLMNGYRRAHFDYAFDYRTIDFDRDNIDSYASFGRTFDLFADGSIHLAF
                     TPGHSPGHMSIVCRLAEHDFVIGGDLVYMLAQLEEHEPGQPRPQDAHNLRRSLQELRL
                     FRRQF"
     gene            892..>1602
                     /locus_tag="VGB57_04775"
     CDS             892..>1602
                     /locus_tag="VGB57_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0130699.1"
                     /translation="MAESSHSQVFRDDLLAGQVCVVSGAGSGLGRETALELARLGATV
                     VACGRREEPLAETAELAAALPGAFEHETLDIREEEPVGRFFDGLLERHGRLDVLVNNA
                     GGQFLSPAEAITPKGFRTVIDLNVQGTWLMTHAAATKAFIPQGGGKVLSVTLSPHNGM
                     PGMVHSGAARAAVENMMRTLAVEWARFGIKTVALAAGQFATETLLTKYPQVVVDNLER
                     SIPIGRAGRPEEMAWLVAY"
BASE COUNT          240 a          519 c          602 g          241 t
ORIGIN      
        1 gaactgacgg cggaagaggc gcagctcctg cagggagcgg cggaggttgt gcgcgtcctg
       61 ggggcgcggc tggccgggct cgtgctcttc gagctgggcc agcatgtaca cgaggtcgcc
      121 gccgatcacg aagtcgtgct ctgcaaggcg gcagacgatc gacatgtggc cggggctgtg
      181 gccgggggtg aaggcgaggt ggatcgagcc gtcggcgaaa aggtcgaagg tgcgcccgaa
      241 gctggcgtag gagtcgatgt tgtcgcggtc gaagtcgatc gtgcggtagt cgaaggcgta
      301 gtcgaagtgg gcgcggcggt agccgttcat caacgggctg ggtccctggg cggcggcttt
      361 ccattcggcc tcgctgacga cgaaagtcga gttggggaac tcggagatcg ccgaactgtg
      421 gtcgaggtgg aggtgggtca tcaccacgat cgggacctcg ctcggctcca gcccgcgctt
      481 gcgcagctgc gcgggcacgt cttcgccggg ctcgagggag gggtggccga agcgggtgcc
      541 gagggagccg aagttctcgc gggcatcgct ggcgacagac gggtgcaagc cggtgtcgac
      601 caggatcgcc ccggcgctcg ggtggcggat caggaaggcc ggcaccggca ccgcgaccgc
      661 gttgcggccg gtcaggatcg agcgcagcag cttcatggtc agcaggcggc cgccggggct
      721 gaccatcatg tcccgcgacc attcgacgtg gcccgcgatc agcggttcga ccgcgacggt
      781 ggcgccgcgg gtgccgccgg cgagcggctc gtgcaggggg gtgggttcga ccgagacgct
      841 catcaaccgg ccaatttacg cagctctgct cgcctatagg cttgcccgcg gatggctgaa
      901 agctcgcatt cgcaggtgtt cagggacgat cttctcgcgg gccaggtctg cgtcgtctcc
      961 ggtgccggca gcggcctcgg gcgggagacg gcgctggagc tggcgcggct cggggccacc
     1021 gtcgtcgcct gtggccgccg cgaagagccg ctggccgaga ccgccgagct cgccgccgcg
     1081 ttgcccggcg ccttcgagca cgagacgctc gacatccgcg aggaggagcc ggtcgggcgc
     1141 ttcttcgacg gtctgctcga gcgccacggc cgtctcgacg tgctcgtcaa caacgccggc
     1201 ggccagttcc tcagcccggc cgaggcgatc accccgaaag gcttccggac ggtgatcgac
     1261 ctcaacgtcc agggaacctg gctgatgact cacgccgcgg cgacgaaggc gttcatccca
     1321 cagggcggcg gcaaggtctt gagcgtcacc ctctcacccc acaacgggat gccggggatg
     1381 gtccactccg gcgccgcccg cgccgcggtc gagaacatga tgcgcaccct cgccgtcgag
     1441 tgggcccgct tcgggatcaa gaccgtcgcc ctcgccgccg ggcagttcgc gaccgagacc
     1501 ctgctgacca agtatccgca ggtggtggtc gacaacctcg agcgctcgat cccgatcggc
     1561 cgcgccggcc ggcccgaaga gatggcctgg ctcgtcgcct ac
//
LOCUS       DASWNU010000243         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_424374, whole genome shotgun sequence.
ACCESSION   DASWNU010000243 DASWNU010000000
VERSION     DASWNU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424374"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1182
                     /locus_tag="VGB57_04780"
     CDS             64..1182
                     /locus_tag="VGB57_04780"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0130700.1"
                     /translation="MHRREFLAATSAAAALLALPASARTRPRGSGPGRIWVDALGGID
                     GYDEKDGRLAPSARLIEAVEAGWINVVSQTIGEVGNGPDRFRSGVEGIAFMDRFVAEM
                     PSLFLKATSVADLRAAKAAGKVAIVYNFQDTTALEADLGRVATFKALGLRILQLTYNK
                     RNLAGDGCLERANAGLSDFGRQAIAEIERNRLLLDLSHAGQRTTAEGIAEAKRPLAIT
                     HTGCRDLVDLPRNQYDRELRALADKGGVVGIYLMPFLRAKGQPGREDLIRHIEHAVKT
                     CGEDHVGIGTDNPLTGYTIDDAARKAQREFFENRSKRGIAAPGEAADVFNLVEGYNEA
                     PRYSRLADDLAARGWKAARIEKVLGGNFVRLFGEAWGG"
     gene            complement(1401..2000)
                     /gene="ruvA"
                     /locus_tag="VGB57_04785"
     CDS             complement(1401..2000)
                     /gene="ruvA"
                     /locus_tag="VGB57_04785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY0130701.1"
                     /translation="MIARLRGLLDGFAADHAVVDVNGVGYLVFASTRTLAALGPVGGE
                     VVLHTVMQVSDDDIRLIGFASGDERDWFRLLTSVQGVGARVALAILSTLAGDDLHRAV
                     AGGDHAMIARSPGVGPKLAQRVVNELKDKVGALALGPATAALSAAPGSAAADAVSALL
                     NLGFRPGEAAATVAKAEAELGPDATLDALVRTALKRAAR"
BASE COUNT          361 a          735 c          796 g          338 t
ORIGIN      
        1 cccctccacc acccttcggg tggtccccct ccccaccggc tccgccggca gggaggcaag
       61 tagatgcacc gtcgtgaatt cctggccgcc acaagcgccg ccgccgcctt gctcgccctc
      121 cccgcctccg cccggacccg cccccgcggc tccggcccgg gcaggatctg ggtggatgcc
      181 ctgggcggga tcgacggata tgacgagaag gacgggcggc tggcgccttc cgccaggctg
      241 atcgaggcgg tcgaggcggg ctggatcaac gtcgtcagcc agacgatcgg agaggtcggc
      301 aacgggccgg accgcttccg ctcgggggtc gagggcatcg ccttcatgga ccgcttcgtc
      361 gccgagatgc ccagcctttt cctaaaggcg acgagcgtcg ccgacctgcg tgcggccaag
      421 gcggcgggca aggtcgccat cgtctacaat ttccaggaca cgaccgcgct cgaagcggac
      481 ctcggcaggg tggcgacgtt caaggcgctc gggcttcgaa ttctccagct cacctacaac
      541 aagcgcaacc tcgccggcga cggctgcctc gagcgggcca atgccggact ctccgatttc
      601 ggtcggcagg cgatcgcgga gatcgagcgc aacagattgc ttctcgacct cagccatgcg
      661 gggcagcgca ccaccgccga gggaatcgcg gaagcgaaga ggccgctcgc catcacccat
      721 accggctgcc gcgacctcgt cgacctgccg cgcaaccaat atgaccgcga gcttcgcgct
      781 ttggccgaca agggcggggt ggtcgggatc tacctgatgc ccttcctccg ggcgaagggg
      841 cagccggggc gggaggacct catccgccac atcgagcatg cggtgaagac atgcggcgag
      901 gaccatgtcg ggatcggcac cgacaatccg ctgaccggct acacgatcga cgacgccgcg
      961 cgcaaagccc agcgcgagtt cttcgagaac cggagcaagc ggggaatcgc cgccccgggc
     1021 gaagcggcgg acgtgttcaa cctcgtcgaa ggctataacg aggcgccgcg ctattcgagg
     1081 ctcgccgacg acctggcggc gcggggctgg aaggcggcgc gaatcgagaa ggtgctgggc
     1141 ggcaatttcg tccggctgtt cggcgaggcc tggggcggct gaacggccaa gatgttcttt
     1201 ttatgttctt ttattgaagg agatttccgg gtctcgattt gagagacttg agtcgggccg
     1261 actcgagttc ggccgggctc tttgacattg tgagattcgt agcaggctcg tcattgcgag
     1321 gagcggagca acgaagcagt ccagccggcg gcgctcgcgg aggcccgact ggattgcttc
     1381 gctgcgctcg caatgacgct tcagcgagcc gccctcttca gcgctgtacg aaccagggcg
     1441 tcgagggtgg cgtccggccc gagctccgct tcggccttgg cgaccgtggc cgcggcttcg
     1501 ccggggcgga agccgaggtt gaggagggcc gagacggcgt cggcggcggc gctgcccggc
     1561 gcggcggaca aggcggcggt ggctgggccg agggccaggg ccccgacctt gtccttgagc
     1621 tcgttgacga ctcgctgcgc gagcttgggt ccgactccgg gggagcgggc gatcatcgcg
     1681 tggtcgccgc cggcgacggc gcggtgaaga tcgtccccgg cgagcgtcga aaggatggcg
     1741 agcgcgaccc gggcgccgac gccctggacc gaggtcagca gccggaacca gtcgcgctcg
     1801 tctccggagg cgaagccgat cagccggatg tcgtcgtcgc tgacctgcat gacggtgtgg
     1861 agcacgacct cgccgccgac cgggcccaaa gcggcgagcg ttctggtcga ggcgaagacg
     1921 aggtagccga ccccgttgac gtcgaccacg gcatggtcgg cggcgaagcc gtcgagcagt
     1981 ccgcgcagcc tggcgatcac aagcgggaga tggggctggc gtgggacatg cccgtttccc
     2041 taccggttcg agccggcctt gtcgaagggg tggcgccgcc agtcccgtca ttgcgagcgc
     2101 agcgaagcaa tccagtgcgg ccgtggagcc ccttggattg cttcgtcgct acgctcctcg
     2161 caatgacgtc agggaacagc gacccgccca agcgcctcgc gcatcgcggc gcggaattcg
     2221 ggccggtcgt
//
LOCUS       DASWNU010000244         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_268504, whole genome shotgun sequence.
ACCESSION   DASWNU010000244 DASWNU010000000
VERSION     DASWNU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268504"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VGB57_04790"
     CDS             complement(<1..1261)
                     /locus_tag="VGB57_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0130702.1"
                     /translation="MRIACLGGGPAGLYFAISMKLRDPAHDIDLYERNRSDDTFGWGV
                     VFSDQTVENLMANDPVSGAVIQSEFAHWDDIEVHIHGETMRSSGHGFIGIGRKRLLNI
                     LQHRARELGVRLHFEHEASADLDSYSDYDLVIAADGANSKIRTAHEARFGVDVQVRKN
                     KFFWFGTEKVFEAFTFAFEETEAGWVWAHAYRFDSGLSTFIVEMAPDTWERLGFDRMD
                     QPDAIALCERIFARYLDGRALMSNAAHLKGPEAWLRFRRILCERWSYRNLILLGDAAH
                     TAHFSIGSGTKLALEDAIKLAEVLNRPGLSREAALGEYREERQIEVLKLQNSARNSTE
                     WFETLERYLHFEPIQFAYSLLTRSQRVSHENLRLRDKPWLEGVERWFQSKASGVPVND
                     PAPPMFAPYRLRALRLANRVVVSPMAMY"
     gene            complement(1322..1621)
                     /locus_tag="VGB57_04795"
     CDS             complement(1322..1621)
                     /locus_tag="VGB57_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY0130703.1"
                     /translation="MTVYVVAQIRIHDRERYDAYAAAFMPVLIQYGGRLLAADEAPAV
                     LEGEWAGQKLNIISFPDEQAARRWMQSEEYRAITVDRLAASDGVVLLVQGFGREA"
     gene            1723..2358
                     /locus_tag="VGB57_04800"
     CDS             1723..2358
                     /locus_tag="VGB57_04800"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0130704.1"
                     /translation="MRWIAAGLMLLALILVPFLLLEAQILEFADALFGGASGQGGALL
                     ALVIVALFAGDAVLPVPSSLVSVAAVALFGWAGGLLIWIGMTIGCGLGYWLGSQAGRP
                     LAQRFIGPGEVARAQRIAADLGLATLVLTRAVPVLAEAATVSAGLAAMPFGRFALATG
                     LANAGIAIVYAVWGAAAAGSAGSFLMLFTAAIAVPALGWIALRLFTRLRTA"
BASE COUNT          392 a          785 c          869 g          436 t
ORIGIN      
        1 agtacattgc catcggagag acgacgacac ggttggcgag gcgcagtgcg cggagccggt
       61 agggcgcgaa catcggcggc gccgggtcgt tgacggggac tcccgaggcc ttcgactgga
      121 accagcgctc gaccccctcc agccagggct tgtcgcggag gcgcagattc tcgtggctta
      181 cgcgctggga gcgggtgaga agcgagtaag cgaactggat cggctcgaaa tggagataac
      241 gctccagggt ctcgaaccat tcggtcgagt tgcgggcgct gttctggagc ttgagcacct
      301 cgatctgccg ttcctcgcga tattcgccca gggccgcctc gcgagagagg cccggccggt
      361 tcaggacctc ggccagcttg atggcgtcct cgagggcgag cttggtcccc gagccgatcg
      421 agaaatgggc ggtgtgggcg gcgtcgccga gcaggatcag attgcggtac gaccagcgct
      481 cgcagaggat gcggcggaac cggagccacg cctccggccc cttcaggtgg gcggcgttgg
      541 acatcagggc ccggccgtcc agatagcggg cgaagatccg ctcgcagagc gcgatggcgt
      601 cgggctggtc catccggtcg aagccgagcc gctcccaagt gtccggcgcc atctcgacga
      661 tgaaggtgga gagcccggaa tcgaaccgat aggcgtgcgc ccagacccag ccggcctcgg
      721 tctcctcgaa ggcgaaggtg aaggcctcga acaccttctc ggtgccgaac cagaagaatt
      781 tgttcttccg gacctggacg tcgactccga aacgagcctc gtgagcggtt cggattttgc
      841 tgttcgcgcc atcggccgcg atgaccaggt cataatccga ataggagtcg aggtcggcgc
      901 tcgcctcatg ctcgaagtgc agccggactc cgagctcgcg ggcgcggtgc tggaggatgt
      961 tcagaaggcg cttgcggccg atgccgatga agccgtgacc cgacgagcgc attgtctcgc
     1021 catggatgtg gacctcgata tcgtcccaat gggcgaattc gctttggatg acggcgcccg
     1081 agacgggatc gttggccatc aggttctcga ccgtctggtc ggagaagacc accccccagc
     1141 cgaatgtgtc gtcgctccgg ttgcgctcat agaggtcgat gtcgtgcgcg gggtcgcgca
     1201 gcttcatcga gatcgcgaaa tagagcccgg ccggtccccc gcccaggcat gcgatgcgca
     1261 tcttttctcc tcctgacgcg aaggggtaaa gcgaccggat gggggaggca agggtgggtg
     1321 cttaggcttc ccggccgaag ccttgaacga gaagcacgac gccgtcggaa gcggccagcc
     1381 tgtcgaccgt gatcgcccga tattcctccg actgcatcca gcggcgcgcg gcctgctcat
     1441 ccgggaagct gatgatattg agcttctgcc cggcccattc tccctcgagg acggccggcg
     1501 cctcgtccgc ggcgagcagg cgtccgccat attggatcag caccggcatg aaagcggctg
     1561 cataagcgtc gtaacgctcc cggtcgtgga tccgaatctg ggcgacgacg tagacggtca
     1621 atgacaatct ccttgtcgat tgccgacgat ggttggtcgg ccggggcgag atgcggtagc
     1681 gtagcgcaat ctaatcgatc gggaggcgcc atggcagggc agttgagatg gattgcggcc
     1741 gggctgatgc tcctcgccct gatcctggtg cccttcctgc tgctcgaggc gcagatcctc
     1801 gaatttgcgg atgccctgtt cggcggcgca agcgggcagg gcggcgcgct gctcgcgctc
     1861 gtcatcgtcg ccttgttcgc cggcgatgcc gttctgccgg tgccttccag cctggtcagc
     1921 gtcgcggccg tggccctgtt cgggtgggcc ggcgggcttc tgatctggat cgggatgacg
     1981 atcggatgcg gcctcggcta ctggctcggc agccaggcgg gccggccgct ggcgcaacgc
     2041 ttcatcggcc ccggggaggt cgcccgcgct cagcgcatag ccgcggacct cgggctggcg
     2101 accctggtgc tgacccgcgc cgtaccggtc ctggcggaag ccgcgaccgt gtccgcgggg
     2161 ctggcggcga tgccgttcgg acggttcgcg cttgccaccg ggctcgccaa cgcgggcatc
     2221 gcgatcgtct atgccgtctg gggcgccgcc gccgccggct cggcgggctc cttcctgatg
     2281 ctctttaccg ccgcgatcgc ggttccggca ctgggctgga tcgcgctccg gctcttcacc
     2341 cggctccgca ccgcctgaag cgcgcaaaga aaaagcgccc cggccggagc cggagcgctt
     2401 tccctcggtg cagccggagc cgcgcctgtt tacatgatga agtcgctggt cgtgagaacc
     2461 ggcgagttga cgaagacgat cg
//
LOCUS       DASWNU010000245         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422 k141_9924,
            whole genome shotgun sequence.
ACCESSION   DASWNU010000245 DASWNU010000000
VERSION     DASWNU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9924"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VGB57_04805"
     CDS             <1..1397
                     /locus_tag="VGB57_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0130705.1"
                     /translation="LYEEIDELGFSVGVALSQDESLIFISTGDNSTSEIRFVPAGDPT
                     APLTLISPRLEKRQYSADAAHGKLWIRTNDEHVNFRIAEADLAAPGDWRTVIPGSDRV
                     YLRGHTSYRDHLAISERLDGLDQLRLRSYDGSEERIPFGEAAYSARFGNNPDFAPDAY
                     RLQYSSMVTPTTIYDYHPAERRLEVLKVQEIPSGYDPSRYETERLMLPARDGKTVPVS
                     IVYRKGFSKDGEGKLFLYAYGAYGYAVPPSFSTSAISLLDRGYAYAIAHIRGGDDLGY
                     DWFLQGKLENRVNTFNDFVDVARGLIAEGFSRPGRIAISGGSAGGELMGVAVNTDPSL
                     WGAVVADVPFVDVLNTMLDESLPLTPGEWTEWGNPIEDKAAFELIRGYSPYDQVRPQA
                     YPPMLITGGLNDPRVTYWEPAKWAAKLRATRTDDNLLLLKINMGAGHGGKSGRWERIR
                     ELAEAYAFVLTQIG"
     gene            complement(1511..2515)
                     /locus_tag="VGB57_04810"
     CDS             complement(1511..2515)
                     /locus_tag="VGB57_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0130706.1"
                     /translation="MATHLPPLSISVALATYNGARFLPEQLASLVAQHRRPDELVVCD
                     DRSADGTVELLEEFARTAPFPVRIHRNESNLGVLRNFEKALSLCEGDIVFLSDQDDVW
                     LPEKISEVVAVFEKTPGALAVINDKLIADENLVPTGATMLGNIRGFGSPDANFVAGCC
                     SAFRREWLSIALPIPRGAIAHDTWLVGLAHRLQVASINEKPLQYYRRHGTNVSQNAYS
                     DQRRVGLARRLAIEIGGTLKRSRDDGGAYWQGFLDSNDAEARRIGERRDDLDRLGLAA
                     QADAAVSLLRRQSETMAARRQLARAPLPRRAAQVWKLWRSGGYAAFSGWKSALKDLLQ
                     "
     gene            complement(2566..3219)
                     /locus_tag="VGB57_04815"
     CDS             complement(2566..3219)
                     /locus_tag="VGB57_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0130707.1"
                     /translation="MASSAKSRWSVSRLARGARNRTARALGAVSARLRRLRTPRLLKE
                     YDRLHLGSGGRLLDGWANVDINGLGNLVWDLTKPLPIPPGRVRFVYSEHFIEHIPHAA
                     ARRLLGHARRVMAADGVIRISTPDLKPLVDDYQAGRLVRMDHADWRPATLCQMVNEAM
                     RNWGHVFIYDEAELTALLRECGFNDVRRVKRGESEFPELRGLESRPDFGDLILEARV"
     gene            complement(3282..>3960)
                     /locus_tag="VGB57_04820"
     CDS             complement(3282..>3960)
                     /locus_tag="VGB57_04820"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY0130708.1"
                     /translation="RIALPLFAARAAEPDALQRGLKMAIGLAMMINLPAMVALAFLSP
                     EVIVVLFGAKWLPAAPILSILAIGGILFPLHAINLQMVLAQGRTRIYLRNEIAKKMIG
                     VTCMLVGSLFGIVGLAWSQVAYSLLAAIVNTRPSHVSLGYGLSKQLRDLSGIFLATLA
                     MAAGIVLLKTLLDLDPLPTLGICSLAGGLLYLLTGFLVRSASFADALSLLPLHRLRAS
                     PAGGDGS"
BASE COUNT          685 a         1316 c         1313 g          646 t
ORIGIN      
        1 ccctctacga ggaaatcgat gagctgggct tcagcgtcgg agtggccctg tcgcaggacg
       61 aaagtctcat cttcatttca acgggcgaca acagcaccag cgagatcagg ttcgtccccg
      121 ccggcgaccc gacggcgcct ctcaccctca tctcgccgcg gctggagaaa cggcaatatt
      181 cggccgacgc cgcccacggc aagctttgga tccggaccaa tgacgagcat gtgaacttcc
      241 ggatcgccga ggcggatctc gccgctccgg gcgattggag gaccgtcatc cccggctcgg
      301 accgggtcta tcttcgtgga cacacctcct atcgcgacca tctcgccata tccgagcggc
      361 tggacggcct cgaccagctc cgcttgcgct cctacgacgg aagcgaggag cggatcccgt
      421 tcggagaggc cgcttattcg gcgcgcttcg gcaacaatcc cgatttcgcg cccgatgctt
      481 accggctcca atattcctcg atggtcactc ccacgacgat ctacgattat catccggcgg
      541 agcgccggct ggaagtgctc aaggtccagg agatcccctc gggctacgat ccctcccgat
      601 atgagaccga gcggctgatg cttccggccc gggacggcaa gacggtgccg gtctcgatcg
      661 tctaccgcaa aggcttcagc aaggacggcg agggcaagct cttcctctac gcctacggcg
      721 cctacggcta cgccgtgccg ccgagcttct cgacgagcgc gatcagcctc ctcgaccggg
      781 gctacgctta tgccatcgcc catatccgcg gcggcgacga cctcggctac gactggttcc
      841 tgcagggcaa gctcgagaac agggtcaaca ccttcaacga cttcgtcgac gtcgcccgag
      901 gcctgatcgc cgagggcttc agccgtccgg gcaggatcgc catttcgggg ggctcggccg
      961 gcggcgagct gatgggagtg gcggtcaaca ccgatccttc gttgtggggg gcggtggtcg
     1021 ccgacgtgcc cttcgtcgac gtcctcaaca ccatgctgga cgagagcctt ccgctcacgc
     1081 ccggcgagtg gaccgaatgg ggcaatccga tcgaggacaa ggccgcgttc gagctgatcc
     1141 gcggctacag cccctacgac caggtcaggc cccaggccta tccgccgatg ctcatcaccg
     1201 gcggcctcaa cgacccccgg gtcacctatt gggagccggc caaatgggca gcgaagctcc
     1261 gcgccacccg gaccgacgac aatctccttc tcctcaagat caacatgggc gccggccatg
     1321 gcggcaaatc gggccgctgg gagcgaatcc gcgagctcgc cgaagcctat gccttcgtcc
     1381 tgacccagat aggttagcag catcgcgagg cgcctccgcc cccccggtca tcctgaactc
     1441 gtttcaggat ccagcgtgcg ttcgtcccgc agcctgaccg gccgggctgg ctgagagacc
     1501 cacgctggcc ctattgcagc aggtccttga gcgcgctctt ccagcccgag aaggcggcgt
     1561 agccgcccga gcgccacaat ttccagacct gggccgcgcg gcgcgggagc ggcgcccgcg
     1621 cgagctgccg gcgcgccgcc atggtctcgc tctgccggcg aagcaggctc accgcggcgt
     1681 ccgcctgggc tgcaaggccg agccggtcga gatcgtcccg ccgctcgccg attcgccgcg
     1741 cctcggcgtc gttcgagtcg aggaagcctt gccaataggc gccgccgtcg tccctggaac
     1801 gcttcagggt tccgccgatc tcgatggcca ggcgccgggc gaggcccacc cgccgctggt
     1861 cgctataggc gttctgcgac acgttggtcc cgtgccgccg gtaatattga agcggctttt
     1921 cgttgatcga ggcgacctgg aggcgatggg cgaggccgac cagccaggtg tcgtgggcga
     1981 tcgcgcctct cgggatgggc aaggcgatgc tcagccattc gcgcctgaag gcggagcagc
     2041 agccggcgac gaaattcgcg tccggcgagc cgaagccgcg aatgttgccg agcatggtcg
     2101 ctccggtcgg gacgaggttc tcgtcggcga tcagcttgtc gttgatgacg gcgagcgcgc
     2161 cgggggtctt ctcgaagacc gcgacgacct cgctgatctt ctccggaagc cagacatcgt
     2221 cctggtccga caggaagacg atgtcgccct cgcacagcga cagcgccttc tcgaagttgc
     2281 ggagcactcc gagattgctc tcgttgcggt ggatccggac cgggaaggga gcggttcggg
     2341 cgaattcctc gagcagctcg acggttccgt cggccgagcg gtcgtcgcac acgacgagct
     2401 cgtccggccg ccgatgctga gccaccaggc tcgccagttg ctccggaagg aagcgagcgc
     2461 cgttataggt cgccagcgcg acggagatgg acaagggggg caggtgggtg gccatggctt
     2521 gggatcgagc ctgtcatgat gcggcggcgc gcctttatgg tgcggctaga cccgggcctc
     2581 aaggatcaga tcgccgaaat cgggacggct ttccaggccg cgcagctcgg ggaattcgct
     2641 ttcgcctcgt ttgacccggc ggacgtcgtt gaagccgcac tcgcgcagca gcgcggtcag
     2701 ctcggcctcg tcgtagatga agacgtggcc ccagttgcgc atcgcctcgt tgaccatctg
     2761 gcagagcgtc gcgggccgcc agtcggcatg gtccatccgg accagtcggc ctgcctgata
     2821 atcgtcgacg aggggcttga ggtccggagt cgagatccgg atcaccccgt ccgccgccat
     2881 cacccgtcgg gcgtggccga gcagccgccg ggcggcggcg tgggggatat gctcgatgaa
     2941 atgctcggaa taaacaaacc gcacccggcc cggcggaatg ggcagcggct tggtcaggtc
     3001 ccagacgaga ttgccgaggc cgttgatgtc gacattggcc cagccgtcga gcagccggcc
     3061 gccgctgccc aggtgaagcc ggtcatattc cttgagaagc cggggcgtcc ggagccggcg
     3121 cagacgcgcc gagacggcgc cgagcgccct cgccgtccgg ttccttgcgc ctcttgccag
     3181 gcgggagacg gaccacctgg atttggccga acttgccatg ataggtatcg actttcctga
     3241 tttgggaatc ctgtaagttg ccccctttgc cgcgacaacc cctatgatcc gtcgcctccg
     3301 gccggtgaag cgcgaagccg gtgcaggggc aggagggaaa gggcgtctgc gaacgaagcg
     3361 gaccgcacga ggaaaccggt cagcaggtag agcagcccgc cggcgaggct gcagatgccc
     3421 agcgtcggca gcggatcgag gtcgagcagg gtcttcagaa gaacgatgcc cgccgccatc
     3481 gccaaggtcg ccaggaaaat accggaaagg tcgcgcagct gcttgctcag gccgtagccg
     3541 aggctgacat gtgagggccg ggtgttcacg atcgccgcca agaggctgta ggccacctgg
     3601 ctccaggcaa ggccgacgat tccgaacagg ctgcccacca gcatgcaggt cacgccgatc
     3661 atcttcttgg cgatttcgtt gcgcaggtag attcgcgtcc gcccctgcgc gagtaccatc
     3721 tgcaggttga tagcgtgcag gggaaagagg attcccccga tcgccaggat cgacaggatc
     3781 ggcgcggccg gaagccattt ggcgccgaac aggacgacga tgacctccgg cgagaggaag
     3841 gcgagcgcca ccatggccgg caggttgatc atcatcgcca gcccgatcgc catcttcagc
     3901 ccgcgctgca gggcatccgg ctccgccgct cgggcggcga agagcggaag cgcgatgcgg
//
LOCUS       DASWNU010000246         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_268772, whole genome shotgun sequence.
ACCESSION   DASWNU010000246 DASWNU010000000
VERSION     DASWNU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268772"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGB57_04825"
     CDS             complement(<1..374)
                     /locus_tag="VGB57_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0130709.1"
                     /translation="MDQSQLRIAILLPCYNEEAAVAQTVAAFRAALPTARLYVYDNNS
                     RDRTREVAEGAGAIVREERMQGKGHVVRRMFADIEADVYVMADGDSTYDAAAAPELVR
                     RLVEERLDMVVGARKSEVEEAYR"
     gene            complement(462..2114)
                     /gene="tig"
                     /locus_tag="VGB57_04830"
     CDS             complement(462..2114)
                     /gene="tig"
                     /locus_tag="VGB57_04830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0130710.1"
                     /translation="MQTVETLNQGLKRAYRITIAKKDIDARVDGELKKVAPQIRMPGF
                     RPGKVPANLVRKMHGAAIEQEALNTAVQEGVQQLIAEQKLRPAMNPHVELEEGGPGKD
                     AVVKVEIETLPEVPAPRIDDLKLERLLVEPTPEEVDAAIARLVEGQKSFEPAPAGEAA
                     EEGDLVVIDYEGSVDGEPFEGGKGESMRVELGSGRLIPGFEDQLVGAKANEQRTLNVT
                     FPEEYPVPYLKGREARFEVTVGEVQKPRSTQMDDSFAQSMGLEGIDQLRELMKGQVEQ
                     ELGGLTRTHMKRRLLDQLAESHDFEVPASMVEAEFNQIWEQLEQEAAQEEDPAAARAE
                     MESEREDYRAIAERRVRLGLLLSEIGQRNNIMINDNEMNQLIMQAAQQYRPEDRQKFA
                     EYVRNEPMAAAQLRAPLFEDKVVDFLFSKAQIAERTVSREELQAEIESTDTGHVHGPD
                     CDHGHDHDHAPKAKKKPAGKKAKAEAVPDQPAESDAAPAQPAEAEAKPAKKGKAKAEV
                     AEEAPAAEAPAKKGKAKKAEPAEEAPAAEAPAKKPKAKKKDA"
     gene            complement(2176..2260)
                     /locus_tag="VGB57_04835"
     tRNA            complement(2176..2260)
                     /locus_tag="VGB57_04835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2224..2226),aa:Leu,seq:tag)
BASE COUNT          384 a          893 c          868 g          530 t
ORIGIN      
        1 cgataggctt cctcgacctc cgatttgcgg gccccgacga ccatgtcgag ccgctcctcg
       61 accaggcggc ggacgagctc gggggcggcg gcggcgtcat aggtcgagtc gccgtcggcc
      121 atgacgtaga cgtccgcttc gatgtcggcg aacatccggc ggacgacatg gcccttgccc
      181 tgcatccgct cctcgcggac gatcgctccc gcgccctcgg cgacctcgcg ggtgcggtcg
      241 cggctgttgt tgtcgtagac gtagagccgt gccgtcggca gcgccgcccg aaaggcggcc
      301 accgtctggg cgaccgccgc ctcctcgtta tagcatggaa ggaggattgc gatcctaagc
      361 tgcgactgat ccaccggcgc ccgtcccccc accgttcggc ctgagcgaag gccccttctt
      421 ggtttaagcc aggcgcttcg acaagctcag cgcgaacggg ttcaggcgtc cttcttcttg
      481 gccttgggct tcttggccgg agcctcggcc gcaggagctt cctcggcggg ctcggccttc
      541 ttcgccttgc ccttcttggc gggcgcctcg gcggcgggag cctcctccgc gacctcggcc
      601 ttcgccttgc ccttcttcgc tggcttggct tcggcctcgg cgggctgagc aggcgctgcg
      661 tcggactcgg cgggctgatc aggcacggcc tcggccttgg ccttcttccc cgccggcttc
      721 ttcttggcct tcggcgcatg atcgtggtcg tggccgtggt cgcaatcggg gccgtggacg
      781 tggccggtat cggtcgactc gatttcggcc tggagctcct cgcggctcac cgtgcgctcg
      841 gcgatctgtg ccttcgagaa caggaaatcg acgaccttgt cctcgaacag cggagcgcgc
      901 agctgggctg cggccatcgg ctcgttcctg acgtactcgg cgaacttctg gcgatcttcc
      961 gggcggtatt gctgcgccgc ctgcatgatc agctggttca tctcattgtc gttgatcatg
     1021 atgttgttgc gctggccgat ctcggacagg agcaggccga ggcggacccg gcgctcggcg
     1081 atggcgcgat aatcctcgcg ctcgctctcc atctcggcgc gggccgccgc cggatcctcc
     1141 tcctgggccg cttcctgctc gagctgctcc cagatctggt tgaactcggc ttcgaccatc
     1201 gacgccggca cttcgaaatc atgcgactcg gcgagctggt cgaggagccg gcgcttcata
     1261 tgggtgcggg tgaggccgcc aagctcctgc tcgacctggc ccttcatcag ctcgcgcagc
     1321 tggtcgatgc cttcgagacc catcgactgg gcgaaggaat cgtccatctg ggtcgagcgc
     1381 ggcttctgga cctcgccgac cgtcacttcg aacctggcct cgcgtccctt cagatagggg
     1441 accggatatt cctcggggaa ggtgacgttg agcgtgcgct gctcgttcgc tttggcgccg
     1501 acgagctggt cctcgaagcc cgggatgagc cggccggagc cgagctcgac ccgcatcgat
     1561 tcgcccttgc cgccttcgaa cggctcgccg tcgacgcttc cttcatagtc gatgacgacg
     1621 agatcgcctt cctccgccgc ttcgccggcc ggggccggct cgaagctctt ctggccctcg
     1681 acgagccggg cgatagcggc gtcgacctcc tccggagtcg gctcgacgag aagccgctcg
     1741 agcttcaggt cgtcgatcct gggcgccgga acctcgggca gggtctcgat ctcgaccttg
     1801 accacggcat ccttgccggg gccgccttcc tccagctcga catgcgggtt catcgccggc
     1861 cgcaatttct gctcggcgat cagctgctgg acgccttcct gcacggcggt gttaagcgct
     1921 tcctgctcga tcgcggcgcc gtgcatcttg cgcaccagat tggcgggcac cttgccgggg
     1981 cggaagccgg gcatccggat ctgcggagcg accttcttca gctctccgtc gacgcgggcg
     2041 tcgatgtcct tcttggcaat ggtgatccgg taggcgcgct tgaggccctg gttcaacgtc
     2101 tcgacggttt gcatttgaaa gctcttacct tctcgcggga gtgcggccct gggcgaattt
     2161 cgggcctggc gggactggtg cgggcgaagg gactcgaacc cccacatctt tcgataccag
     2221 gacctaaacc tggcgcgtct accagttccg ccacgcccgc cgggccgccg gtcgcgcggg
     2281 gatatagcag gcgtggcgcg aggagcaagg gcggtggcgg ggagtggggc tcaaggcggc
     2341 ggggaggcag atggtcgttc aagcagaggc gaaggcggaa aggcaagccg atacgacaaa
     2401 atcagtcagt cgagatgcag aagttgcgat aaccaaaact catggtccga acgctccggc
     2461 cgccgaggca acgtacggcc actgttcact tgacttttgc aatccttggg attacaggat
     2521 ttctaatgtt tgagaccgac gacattgccg aggcagggtt gccagcgatg gaccggatcc
     2581 caagtccgca cggcatgcgc taaaagaccc gatagctttt tcggcttcca accaacccct
     2641 ctcaatcacg ccctcgcggc gacgcgccga cgtca
//
LOCUS       DASWNU010000247         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_268878, whole genome shotgun sequence.
ACCESSION   DASWNU010000247 DASWNU010000000
VERSION     DASWNU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268878"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VGB57_04840"
     CDS             complement(<1..588)
                     /locus_tag="VGB57_04840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0130711.1"
                     /translation="MASTVGKISNRVLSVTAGLSAFVLTLLGFLLLGSLNQQVAASVV
                     FGLFALTIVYLAAERPNSDHARAVSALIDRLMAVSRGDLASPAPAALKREMPALAAAV
                     EGLFDQVRSNLDDAQAMAMYDPVTALPNRVHFRREADRILASRDSGAALLFIDLDGFK
                     EVNDRLGHAQGDQVLVMVANRLRVVLKAEVADGTLA"
     gene            complement(684..1223)
                     /locus_tag="VGB57_04845"
     CDS             complement(684..1223)
                     /locus_tag="VGB57_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0130712.1"
                     /translation="MRRLNPEEAKLWAKVVESVRPLDGVAMPESPSSPAEAGAREAPP
                     PGPALRRGATAPPPPAPARKLPGETLDGTWDRRLSRGLVSPDAMLDLHGHNLATAYDL
                     LDRRLEQAIQSGARLMLLVTGKPPGPERPAKRGAIRAAVGDWLQASRHSGRIAAVRNA
                     HPRHGGTGALYIVLRRPRD"
     gene            complement(1204..2394)
                     /locus_tag="VGB57_04850"
     CDS             complement(1204..2394)
                     /locus_tag="VGB57_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEY0130713.1"
                     /translation="MKLPNLSPLLIAMMVLASCVPKERRPAPPVPQLPPVAVPAPPAP
                     AKPPTAALLGVSAGPALPAIGEAEAARALGAFRISCPALVKRTDASGLTRPEDWQAAC
                     AAAATATAPAAFFAEHFEVVQIGDGAAFVTGYFEPEIAGSRTRQPGYEVPVYRKPPDL
                     VEVDAATAEAAGTPRRGRLENGVMVPYFERSDIESGALANRGLEIAWAADEIDFFFLQ
                     IQGSGRLRLPDGGVMRIGYAGQNGREYVGIGKLMKDRGLLGPGQTSMQGIVAWLRAHP
                     EEGRAIMRENKSYIFFQELTGPGPLGALGLPVTGRATVATDPKYVPMGAPVLLQLDRA
                     EANGLWVAQDTGGAIKGSNRFDTFWGAGAEAARLAGGMQGRGRAYVLVPKGTLARLDA
                     TTQP"
     gene            complement(2558..3208)
                     /locus_tag="VGB57_04855"
     CDS             complement(2558..3208)
                     /locus_tag="VGB57_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEY0130714.1"
                     /translation="MTEIVLLAMVALFVGLRLYSVLGRRTGHEQQPILRPAETAATTE
                     AAAPSADLAVDRPDTGGFVYEEPATPGIRAIVGADPAFDVARFLEGAQAAYRMILEAF
                     WRGDREELRHLVGGDVLVTFEEAITAREEAGEQLDNRLVQIERAVIQDARLNGRTAEV
                     DVRFDADIAAVTRDREGKVIAGTLSDAIPTHDVWTFRRNLASGDPNWLLVETDEAD"
     gene            3393..3905
                     /gene="secB"
                     /locus_tag="VGB57_04860"
     CDS             3393..3905
                     /gene="secB"
                     /locus_tag="VGB57_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY0130715.1"
                     /translation="MAEEQGNPAEMQPNGNDTAPQVGVISQYVKDLSFENPNSPAVYQ
                     WQTQPKIEVDFNIGSSKLNDEIYEVALRIEVRATADGQTAFAVDLMYAGLIGMRNVSD
                     EGEIQAFLLAEAPRILFPFARHIIAQTVQEGGFPPLMLDPIDFHALYVSRAQQDLAGE
                     GGAAGEIGQA"
BASE COUNT          629 a         1367 c         1461 g          622 t
ORIGIN      
        1 cgcgagggtc ccgtcggcga cctccgcctt gagcacgacc ctcagccggt tggcgaccat
       61 gacgagtacc tggtcgccct gggcgtggcc gaggcgatcg ttgacctcct tgaagccgtc
      121 gaggtcgatg aacagaagcg ccgcgccgct gtcgcgcgaa gcgaggatcc gatcggcctc
      181 gcgccggaaa tggacccggt tgggaagcgc cgtgacagga tcgtacatcg ccatcgcctg
      241 ggcgtcgtcg aggttggagc ggacctggtc gaacaggcct tcgacggcgg ccgcgagagc
      301 gggcatctcc cgcttcagag cggcgggcgc gggcgaggcg agatcgccgc ggctgacggc
      361 catcagccgg tcgatcagcg ccgagacggc gcgggcgtgg tcgctgttgg gccgctcggc
      421 ggcgaggtag acgatggtca aggcgaagag gccgaagacg accgaggcgg cgacctgctg
      481 gttgaggctg ccgagcagca ggaagcccaa aagcgtcagg acgaaggccg acagccctgc
      541 cgtcaccgag agcacgcgat tgctgatctt tccgaccgtc gaagccatga tcggcggtgt
      601 ttcctgtccg gcctgccgca caagccccca cggcgcgcgt tgctgatgca gattatgccg
      661 gaagcgttac cattcggtat ccatcaatct cgcgggcgcc tgaggacgat gtagagcgcg
      721 cccgtgccgc cgtgtcgggg atgggcgttg cggacggccg cgatccggcc cgagtgccgc
      781 gacgcctgca gccagtcgcc gacggcggcg cggatggcgc cgcgcttcgc cggccgttcg
      841 gggccgggcg gcttgccggt gacgagcagc atcagcctcg ctccggattg gatcgcctgc
      901 tccagccgcc ggtcgagcag gtcgtaggcg gtggcgagat tgtggccgtg aaggtcgagc
      961 atcgcgtccg gcgacacgag gccgcggctc agccggcggt cccaggtccc gtcgagagtc
     1021 tcgcccggga gcttgcgggc tggagcgggt ggcggaggcg cggtcgctcc ccggcgaagg
     1081 gcggggcccg gcggcggcgc ttcccgggcc ccggcctccg ccggggagct tggggactct
     1141 ggcatggcta cgccgtcgag cggccgcacc gattcgacca ccttcgccca caatttcgct
     1201 tcttcagggt tgagtcgtcg catcgagccg cgccagcgtc cccttcggca cgagcacgta
     1261 agcgcggccc ctcccctgca ttccgccggc gagccgggcc gcctcggcgc cggcgcccca
     1321 gaaggtgtcg aagcggttcg agcccttgat cgctcccccc gtatcctgcg ccacccacag
     1381 gccgttcgcc tcggcgcggt cgagctggag aaggacgggg gcgcccatcg gcacatattt
     1441 gggatcggtg gcgaccgtgg cgcggccggt gacggggagg ccgagggcgc cgagcgggcc
     1501 ggggccggtc agctcctgga agaagatgta gctcttgttc tcgcgcatga tcgccctccc
     1561 ctcctcggga tgggcgcgaa gccaggcgac gatgccctgc atgctcgtct ggccggggcc
     1621 gagcagcccc ctgtccttca tcagcttgcc gatgccgaca tattcgcggc cgttctggcc
     1681 ggcatagccg atccgcatca ctccgccgtc aggcagccga agccggccgg agccctggat
     1741 ctggaggaag aagaagtcga tttcgtcggc ggcccaggcg atttcgaggc cgcgattggc
     1801 gagcgccccc gactcgatgt cggaacgctc gaaataaggg accatgaccc cgttctcgag
     1861 ccgcccccgc cgaggagtgc cggccgcctc cgcggtcgcc gcgtcgactt cgacgaggtc
     1921 gggcggcttg cggtagaccg gcacttcata accgggctgg cgggtgcgcg agccggcgat
     1981 ctccggctcg aaatagccgg tgacgaaggc ggcgccgtcg ccgatctgga cgacttcgaa
     2041 atgctccgcg aagaaagcgg ccggcgccgt cgccgtcgcg gcggcggcgc aggccgcctg
     2101 ccaatcctcg ggccgggtga ggccgctggc gtcggtgcgc ttgaccaaag cggggcagct
     2161 gatccggaag gccccgaggg cgcgcgcggc ctcggcctcc cctatggccg ggagagccgg
     2221 tccggcggag actccgagca gggcggcggt ggggggcttg gccggggccg gtggagcggg
     2281 cacggcgacg ggaggaagct gcggcaccgg tggcgcgggc cgcctttcct tggggacgca
     2341 gctggcgagg accatcatgg cgatgaggag cggcgaaagg ttcggaagtt tcatagtttc
     2401 agcttcccag gaaccgaaaa acttccgttc ccagggaaac ccagcctccg atccccccgt
     2461 cctgattcgc tgccgcccat ccggagagga tacccctcgc aggggggtgt aggaaaattg
     2521 tttctgcctc cctgccgcga agcggtgggg aggcaaatca atccgcttcg tcggtctcga
     2581 cgagcagcca gttggggtcg ccgctggcga ggttgcggcg gaaggtccag acgtcgtggg
     2641 tcgggatggc gtcgctcagg gtgccggcga tcaccttgcc ctcgcggtcg cgggtgacgg
     2701 cggcgatgtc ggcgtcgaag cggacgtcca cctcggcggt gcggccattg aggcgggcat
     2761 cctggatcac ggcacgctcg atctgcacga gccggttgtc gagctgctcg ccggcttcct
     2821 cgcgcgcggt aatggcctct tcgaaggtga cgagaacgtc gccgccgacg agatgcctca
     2881 gctcctcccg atcgccgcgc cagaaggctt cgaggatcat ccggtaggcc gcctgggcgc
     2941 cctccaggaa acgggcgacg tcgaaagcgg ggtcggcgcc gacaatggcg cggatgcccg
     3001 gagtcgcggg ctcctcatag acgaatccgc ccgtgtcggg ccggtcgacg gcgaggtcgg
     3061 cgcttggagc ggcggcttcg gtcgtggcgg cggtctcggc cggacgaagg atcggctgct
     3121 gctcatggcc ggtgcggcgg ccaagcaccg agtaaagcct caggcccacg aacagggcca
     3181 ccatcgccag gaggacaatc tcggtcacta gtttctccga actcaacgag cctctacata
     3241 ggctgtccac gtcaaggatt caaatgccgg tcggttccac aggggccgtg ccgaccggtt
     3301 gcgcggtccg gccgcgcctg ctacccgccc gggcgcaccg ggtcccgcgc ctcgcggccc
     3361 gtctccaccc aaactttttc aaggcgaatc aaatggccga agagcagggc aaccccgccg
     3421 agatgcagcc caatggcaac gacaccgcgc ctcaggtcgg agtcatctcc caatatgtga
     3481 aggacctttc cttcgagaat ccgaactcgc cggcggtcta ccagtggcag acccagccca
     3541 agatcgaggt cgacttcaac atcggctcga gcaagctcaa cgacgagatt tacgaagtcg
     3601 cgctgaggat cgaggtccgg gccaccgccg acggccagac cgccttcgcg gtcgacctca
     3661 tgtatgccgg cctgatcggg atgcgcaacg tctccgacga aggcgagatc caggccttcc
     3721 ttctcgccga ggcgccccgg atcctcttcc ccttcgcccg ccacatcatc gcccagacgg
     3781 tgcaggaggg cgggttcccg ccgctgatgc tcgatccgat cgacttccac gccctctacg
     3841 tctcgcgggc gcagcaggat ctggcaggcg agggcggagc ggccggcgag atcggccagg
     3901 cctgaccggg tcggccctgg gggcggcggc atgaacctct ggaaggcgac cgggacgatc
     3961 ggagggctga cgctggtcag ccgcatcctg ggcttcgttc gggaaatggt cgtcgcgcgc
     4021 gtgctcgggg cgtcggcctc ggccgacgtg ttcacgctcg ccttcctgat cccaaacct
//
LOCUS       DASWNU010000248         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_87149, whole genome shotgun sequence.
ACCESSION   DASWNU010000248 DASWNU010000000
VERSION     DASWNU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87149"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGB57_04865"
     CDS             complement(<1..386)
                     /locus_tag="VGB57_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130716.1"
                     /translation="MATKTARASENRSMASKRIRKIFGNIHEVSEMPNLIEVQRESYE
                     QFLRSRPQDSYVSGLEKTLRSVFPIRDFAGTAELDFVHYELEEPKYDTDECRQRGMTY
                     AAPMRVTLRLIVFEVDPDTETRSVLD"
     gene            793..1191
                     /locus_tag="VGB57_04870"
     CDS             793..1191
                     /locus_tag="VGB57_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEY0130717.1"
                     /translation="MNDLPTFGSDREAFIDEAVKSGRYGSRAEVIEEALLLLKRRDAS
                     LAKLDADLARGIADIDAGRTYDLDEAFDRVREELRRRSEQDEAEQLADIRAAVARGFA
                     DVDAGRVIDAEEAFAGLETHFRALAHGYSR"
     gene            1188..1532
                     /locus_tag="VGB57_04875"
     CDS             1188..1532
                     /locus_tag="VGB57_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0130718.1"
                     /translation="MKVRITQAALADLRDIGHWIALDDPERAITFVQELAEKCLSLSD
                     RPSPIRPRPRRVKASGSDGIANPDPLPCRRGDHRRPALSEDVHATVTLFVVAGAPRGL
                     SLTQGFCIIFRG"
     gene            1546..1755
                     /locus_tag="VGB57_04880"
     CDS             1546..1755
                     /locus_tag="VGB57_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130719.1"
                     /translation="MEDRPQLKFDDDDSASGAGSSEREARRAELRAAVKQGLVDIEEG
                     RVVDLGEALDRIEAMLDELEAAKRS"
     gene            2009..2212
                     /locus_tag="VGB57_04885"
     CDS             2009..2212
                     /locus_tag="VGB57_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130720.1"
                     /translation="MPPIAFLALLTITLLVLAAFRLELDFRRSDKERPEPGPAAPRPP
                     ELRAAPAGGYCQAPPPPRLFGRK"
     gene            complement(2228..3172)
                     /locus_tag="VGB57_04890"
     CDS             complement(2228..3172)
                     /locus_tag="VGB57_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEY0130721.1"
                     /translation="MKAAGGLAPLAVAALALAISDGDSPVTAKHHTPSVSVSAGGPAA
                     APIPPRADLPSPPPDFLPAECLKQYQIDFPHHRGDDVARRQTKVCREGYLLSFNVDTQ
                     SPDWVVERLKQADLVGPASRTDKFVDDPALPPEVDADNADYLKTGFDRGHQAPAADAK
                     FSQKVMDESFYFTNMSPQRGIGMNRGAWKFLEEATRSWVLCGGHPDLYVITGPIYGQL
                     EGNPRSIGTPPVAVPRAYYKIVYDANLGRAVGFVLPNTKIGSRIDLQDYVWKIADIET
                     ETGLNFFRTMDQRTQAQMKDNPGVAWQHSGTCPGDTGD"
BASE COUNT          551 a         1037 c         1193 g          579 t
ORIGIN      
        1 atatcgagca ccgaacgggt ctcggtatcg ggatcgacct cgaacacgat gaggcgaagg
       61 gtgacgcgca tcggcgccgc gtaggtcatt ccgcgctggc ggcactcgtc ggtgtcgtat
      121 ttcggctctt cgagctcgta atggacgaaa tcgagctcgg cggtgccggc gaaatcgcgg
      181 atcgggaaga cgctgcgcag ggtcttctcc aggcccgaca cataggaatc ctggggccgc
      241 gagcggagga actgctcgta gctctcgcgc tgaacctcga tgaggttcgg catttcggag
      301 acttcgtgga tgtttccgaa gatcttgcgg atgcgcttgg atgccatcga gcggttttcg
      361 ctggcccggg ccgtcttggt cgccatggag atgtgtctcg ccttcctgtg ccccggcgga
      421 tggccgggat ctcatcgtcg tcccggctcg aaccgggatc cattccgggc ccgcccgcct
      481 cgcgaaagcg agggcaaagc ccgatgcgga acgcagaaga cgcaaaaagc cgcgagtccc
      541 cgatattggg accgcagctt cagcgtcttg aaaacccctt gtcgatcaat gctttgaacg
      601 cgctgcgcga cggcgcgtcc tgttccgata cgatggggtg tgggcgctat gtaggattgt
      661 gaggggcgag ggtcaagggt gaagccctga tgatcgacaa ctaggttcga taaaaacgtt
      721 gaggtaaccg atcttcatat ggggagcttg gccacgttgc ctggccaggc caaagagcct
      781 aaattcgcgg ccgtgaacga cctccccacc ttcggctccg atcgcgaagc ctttatcgat
      841 gaggcggtga aatccggtcg ctacgggtcg cgcgccgagg tgatcgagga ggcgctgctg
      901 ctgctcaagc ggcgtgacgc gagcctcgcg aagctcgacg ccgaccttgc ccgcggaatc
      961 gccgatatcg atgccgggcg cacgtacgat ctggacgagg cgttcgatcg agtgcgcgag
     1021 gagcttcgca ggagaagtga gcaggacgag gctgagcagc ttgcggacat ccgcgccgcg
     1081 gtcgcgcgcg gcttcgcgga tgtcgacgcc gggcgggtca tcgatgccga agaagcgttc
     1141 gccgggttgg aaacgcattt ccgcgcactg gcccacggct acagtcgatg aaggtccgaa
     1201 tcacgcaggc ggcgttagcc gacctgcggg acatcggcca ctggatcgcc ctcgacgacc
     1261 cggagcgggc gatcacgttc gtgcaggagc tggccgaaaa gtgcctgagc ctctcggacc
     1321 ggccttcgcc tatccgaccg cgcccgaggc gggtgaaggc gtcaggaagc gacggcatcg
     1381 caaatcctga tcctctacca tgtcggcgcg gagaccatcg acgtcctgca ctatcggaag
     1441 atgttcacgc gactgttacg cttttcgtag tggcgggcgc cccccgcggg ctgagcctaa
     1501 ctcaaggatt ttgcataatc tttcggggat gataggtgct gccgaatgga agatcgcccg
     1561 caactgaagt tcgacgacga cgatagtgcc tccggcgctg gaagcagcga gcgcgaggcg
     1621 cgccgggccg agctccgcgc cgccgtcaag cagggcctgg tggatatcga ggagggccgg
     1681 gtcgtcgatc ttggcgaagc gctcgaccgg atcgaggcga tgctcgacga gctggaggcg
     1741 gccaagcggt cgtagcccgg gcgtctccgt gacaattgcg acaatttcgc cgccgacgcg
     1801 acatcgtcct gaccgctgcg gccgctaatt ccctctgcaa cggcgccggg caatagcccc
     1861 gcccgatgca aggaaggaat aagcgatggc gtggatcgtg gggctgggcc tgatgatggg
     1921 gacgatggtg accctccagg cgttctgcgc cgagctcagc ttccggctgc gcctcgagca
     1981 gcgcgagcgc tgaggaagga tccggaacat gcccccaatc gccttcctgg ccttgctgac
     2041 catcaccctg ctggtactgg cggccttccg cctcgagctg gacttccgcc gctccgacaa
     2101 ggagcggccc gagcccggtc ccgcggcgcc caggccgccc gagcttcgcg ccgcaccggc
     2161 ggggggttat tgccaggcgc cccctccccc gcggctgttc ggcaggaagt gaaccccgcc
     2221 gccgggctca gtcgccagtg tccccggggc aggtgccgct atgctgccag gcgacgcccg
     2281 gattgtcctt catctgggcc tgggtgcgct ggtccatcgt ccggaagaag ttgaggcccg
     2341 tctcggtctc gatgtcggcg atcttccaca cataatcctg aaggtcgatc cgcgagccga
     2401 tcttggtgtt gggcaggacg aagccgacgg cgcggccgag attggcatcg tagacgatct
     2461 tgtaataggc ccggggcacc gccaccggcg gagtgccgat cgagcgcgga ttgccctcga
     2521 gctggccgta gatcgggccg gtgatcacat agagatccgg atgcccgccg cacagcaccc
     2581 aggagcgggt cgcctcctcg aggaacttcc aggcgccgcg gttcatgccg atgccgcgct
     2641 gcggcgacat gttggtgaag tagaaggatt cgtccatcac cttctggctg aacttggcgt
     2701 cggcggccgg cgcctggtgg ccgcggtcga agccggtctt cagatagtcg gcattgtcgg
     2761 cgtcgacctc gggcggaagc gccgggtcgt cgacgaactt gtcggtccgg ctggccggcc
     2821 cgaccaggtc ggcttgcttg agcctttcca ccacccagtc gggactttgg gtgtcgacgt
     2881 tgaacgaaag cagatagcct tcacggcaga ccttggtctg gcgtcgggcg acgtcgtcgc
     2941 cccggtggtg cgggaagtcg atctggtact gcttcaggca ttcggcgggc aggaagtccg
     3001 gcggtggcga cggaaggtcc gcgcggggtg ggatcggcgc cgccgcgggt cccccggcgg
     3061 agaccgagac ggagggcgtg tggtgcttgg cggtgacggg tgaatccccg tcgctgatgg
     3121 cgagggcaag cgcggccacc gccagcggag cgaggccgcc ggcagccttc accgccttcg
     3181 acgagcgcga atagccggcg gcgggagccg ggctcgctag cggacgaggc ggtggcggtg
     3241 gcggtggcgg tggaggcggc ggtgcgggtg gcggcggagc cggcgcgtcg gccaggattt
     3301 cgccgctccc ggcctcgccg cggtctgccg ccgccgcgtg tttggccagc tcggcggtga
//
LOCUS       DASWNU010000249         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_478019, whole genome shotgun sequence.
ACCESSION   DASWNU010000249 DASWNU010000000
VERSION     DASWNU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478019"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..951)
                     /locus_tag="VGB57_04895"
     CDS             complement(76..951)
                     /locus_tag="VGB57_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0130722.1"
                     /translation="MRLIGLLATLLVFFAQPAWADDSPRLRSLEQRLAAMAAENPGEY
                     GIAALDLASGETISFNGQQAFPMASTMKIAVAAAYLAEVDAGRRTLDDPVAGTSALKL
                     MDAMITRSDNRATDLLMATLGGPRAIDAWLRGHGLNGIRVDRTIAQLLSDRRDLRDVR
                     DSSTPIAMLGLLRLIDAEGALTSQSRYLLRDMMRRCATGSNRIRGILPPGATVEHKTG
                     TLSGYTGDVGFLTTPNGRRIAVAFFARGGSNRPAVIATAARAIYDALGAEHPGTAFSA
                     PAATNPPAGAQTGTK"
     gene            1250..1417
                     /gene="rpmG"
                     /locus_tag="VGB57_04900"
     CDS             1250..1417
                     /gene="rpmG"
                     /locus_tag="VGB57_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY0130723.1"
                     /translation="MAKPTTVKIKLVSTADTGFYYVTKKNPRNITEKMTFRKYDPVVR
                     KHVDFKEAKIK"
     gene            complement(1710..2069)
                     /locus_tag="VGB57_04905"
                     /pseudo
     CDS             complement(1710..2069)
                     /locus_tag="VGB57_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2087..2359
                     /locus_tag="VGB57_04910"
     CDS             2087..2359
                     /locus_tag="VGB57_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HEY0130724.1"
                     /translation="MRRDDTNPTDPEALALSALGWTLGDEARAQRLLALTGLDPSSLR
                     DRLGEPGLLASVLAFLEAHEPDLLAAAEALGVSPAALVEARRRLEA"
     gene            2356..2970
                     /locus_tag="VGB57_04915"
     CDS             2356..2970
                     /locus_tag="VGB57_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0130725.1"
                     /translation="MSGRPLLVTDCDEVLLHMVSHFADWLAEAHGLSFDLSAPGFRDA
                     LRDSGGRPVAAERVWPLLDGFFAGEMHRQNVVPGAVEALAAISAHADIVVLTNIGDEF
                     EAGRVEQLERFEIRHRVLCNRGGKGRPVLELIETMAPSAIVFVDDLAVHHESVSVHAP
                     QAWRLHMIAEPRLAAHTPPAPHAHARIDSWDEAVPWILEWLGAQ"
     gene            2952..3686
                     /locus_tag="VGB57_04920"
     CDS             2952..3686
                     /locus_tag="VGB57_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0130726.1"
                     /translation="MAGGAMIELVPIRYGGGDDVELTGYLADGSKGRPAPGVLVAHEG
                     GGLGRHTKERARRLGELGYVAFALDYYGQEDPPLERAMALGKALRGDRPLFRKRLGAG
                     LDVLLARAEVDPLRLAGIGYCMGGAAVIELARMGAPFVAVAGFHSGFVPASPEENAAI
                     GGKLLLCHGADDPIVGPVQRDAFLAEASAAGVDWQLHLYGGVGHSFTNPDIDALGLPG
                     FAYDETADRRSWAAMLNLFEEVLELR"
     gene            3683..>3856
                     /locus_tag="VGB57_04925"
     CDS             3683..>3856
                     /locus_tag="VGB57_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0130727.1"
                     /translation="MTKTAFITGVTAGFGAATARRFVAASWRVVGTGRRRERLDELAS
                     ELGGGFLPLRLDMR"
BASE COUNT          573 a         1360 c         1266 g          657 t
ORIGIN      
        1 cgatatggtt accgctttgt taaagcgcgc agtgcctcag ccgaaaatcg gctccggcac
       61 ccatcgcgct cctgctcact tggtgccggt ttgagccccg gccggcgggt tcgtggccgc
      121 gggagcgctg aacgccgtcc ccgggtgctc ggctcccagc gcatcgtaga tcgcccgggc
      181 ggccgtggcg atgaccgccg gccgattgga gccgccgcgc gcgaagaagg caaccgcgat
      241 ccggcgtcca ttgggcgtgg tcaggaaacc gacgtcgccc gtatagcccg aaagagtgcc
      301 cgtcttgtgc tccacggtag cgccgggagg aaggatcccg cggatccggt tggagccggt
      361 cgcacaacgc cgcatcatgt cccgcaggag gtagcggctc tgggacgtca gtgcgccctc
      421 tgcgtcgatc agccgaagca ggccgagcat ggcgatcggg gtgctcgagt cgcgaacgtc
      481 gcgcaaatcc cgccggtcgc tgagcaactg cgcgatcgta cggtccaccc ggatgccgtt
      541 gagaccgtgg ccgcgcagcc aggcatcgat ggctctcggc ccaccgagcg ttgccatcag
      601 cagatccgtg gcgcgattgt cgctgcgcgt gatcatcgcg tccatcagct tcaacgcgga
      661 cgtgcccgcg acaggatcgt cgagggtccg ccggccggcg tccacttccg ctagataggc
      721 cgccgcgacg gcgatcttca tcgtgctcgc catcgggaag gcctgctggc cgttgaagct
      781 gatcgtctcc ccgctcgcca ggtcgagcgc cgcgattccg tattcgcccg gattctcggc
      841 ggccatggcc gcgagccgct gctcgagcga gcgcaggcgc gggctgtcgt ccgcccaggc
      901 gggctgcgcg aaaaagacca gaagggtcgc gagcaagcca atcaatcgca tcaaaatccc
      961 ctcagcagcc gcagtggcgt cgaccggctc accatagcgc ccgttcagcg cgatgcgatg
     1021 aacgcacttt ggtaaacacg cgcgttcgtc gcagctttgg ctccatatgg cgcgacggcg
     1081 ggaacatatg cgtccggggt gcggtccagc ccagcttttc cgctccccgg ctacgagggg
     1141 tccccgccct tgacagcggc cctgcttcga ggcagaggcg gccgggcata tgagcggcgg
     1201 ggacgccgct tttatttttt tacgaaaccc cgttttttga gaggctgtca tggccaagcc
     1261 gaccacggtc aagatcaagc tcgtcagcac cgccgacacc ggcttctatt atgtgacgaa
     1321 gaagaatccg cggaacatca ccgagaagat gaccttccgc aaatacgatc cggtcgtgcg
     1381 caagcacgtg gatttcaagg aagccaagat caagtgacgc cgctggcgct gctcagcgcc
     1441 taggcgtcat cccggcgaaa gccgggatcc atgaactccg ttcgtggaat gtcggcttcg
     1501 cctgcgttcg tggatgccgg atcaagtccg gcatgacggt gtttcgaagc gggtgggccg
     1561 ccgcccaacc gtcactctgg acctgcttca gggtccaggt tttcacccgc accgttgctc
     1621 gacgctggat tctcacccgg gtccggcatg ccggcgtcgc cctgcgtctc cacctcctcg
     1681 cttgcgtccg gttccggcct cggcgccgcc agaagcgccc gaaccgcctc cacgaagcgc
     1741 attactgaga tgggcttgga cacatagccc tcagcgccgg cctcgcggat ccgctcttcg
     1801 tcgcctttgg cggcataagc ggtgaccgcc atgatcggcg ttcgcctcag gtcggcatcc
     1861 cccttgagct gctcgatcag ctcgagcccg ctgacatagg gcatctgaat gtccatgacg
     1921 atgagatcgg gccggaaccc ccgcgcgcgc tccacggcct cgcggccgtc gcggaccggc
     1981 tcggcttggt agccatgcgc ccgcaacagg tcgcagaaca gcttcaggtt gagttcgttg
     2041 tcctcgacaa cgaggatttt ttgtcccacc ggcgcacctg tagaagatgc ggcgcgacga
     2101 cacaaacccg accgaccccg aggccctggc cctctccgcc ctcggctgga cgctgggcga
     2161 cgaggcccgc gcgcaacggc tgctcgccct caccggcctc gatccctcgt ccttgcgaga
     2221 tcgcctcggc gagcccgggc tgctcgcctc cgtcctcgcc ttcctcgaag cgcacgagcc
     2281 cgatctcctc gccgcggccg aggcgttggg cgtgtctccg gcggctctgg tcgaggctcg
     2341 gcggaggctc gaggcatgag cggccggccc ctgctcgtca ccgactgcga cgaggtcctg
     2401 ctccacatgg tctcgcattt cgccgactgg ttggcggaag cgcatggcct gagcttcgat
     2461 ctttccgccc ccggttttcg cgacgcgctt cgcgattcgg gcgggcggcc ggtcgccgcc
     2521 gagcgggtat ggccgctgct cgacggtttc ttcgccggcg agatgcaccg gcagaacgtc
     2581 gttccgggtg cggtcgaggc cctggcggcc atttccgcgc atgccgacat cgtcgtcctc
     2641 accaatattg gcgacgaatt cgaggcggga cgggtcgagc agctcgagcg gttcgagatc
     2701 cgccaccggg tgctgtgcaa ccgcggcggc aaggggcggc cggtgctcga gctgatcgag
     2761 accatggcgc cgagcgcgat cgtgttcgtg gacgatttgg ccgttcacca cgaatcggtg
     2821 tcggtccacg ccccccaggc ctggcgcctg cacatgatcg ccgaaccgcg cctcgccgcg
     2881 cacacgccgc ccgcccctca cgcccatgcc cggatcgatt cctgggacga ggcggtgccc
     2941 tggatcctcg aatggctggg ggcgcaatga tcgagctggt cccgatccgc tacggcggcg
     3001 gcgacgacgt cgagcttacc ggctacctcg ccgacggctc caagggccgc cctgcaccgg
     3061 gcgtgctggt ggcccatgaa ggcggcgggc tcggccggca cacgaaggag cgcgcacgcc
     3121 ggctcgggga gctgggctat gtcgccttcg ccctggatta ttatgggcaa gaggatccgc
     3181 cgctcgagcg ggccatggcg ctgggcaagg cgctgcgcgg cgaccggccg ctcttccgca
     3241 agcgcctcgg cgccggcctg gacgtgcttc ttgcccgggc ggaggtcgac cccctccggc
     3301 ttgccgggat cggctattgc atgggcggag ccgccgtcat cgaactggcc cgaatgggag
     3361 cgcctttcgt tgccgtcgcc ggcttccaca gcggcttcgt gccggcctcg ccggaggaga
     3421 atgccgccat cgggggcaag ctcctgctct gccacggcgc cgacgacccc atcgtcggcc
     3481 cggtccagcg cgacgccttt ctcgccgaag cttcggcggc cggggtggac tggcagctcc
     3541 acctctatgg cggggtcggc cacagcttca ccaaccccga catcgatgcc ctcggcctgc
     3601 ccggcttcgc ctatgacgaa acggccgacc ggcgatcctg ggcggcgatg ctcaacctgt
     3661 tcgaggaagt tctggagctc cgatgaccaa gaccgctttc atcaccggcg tcaccgccgg
     3721 cttcggcgcc gccaccgccc gccgcttcgt cgccgccagc tggcgcgtgg tcggtaccgg
     3781 ccgccgccgc gagcgcctcg acgagctcgc cagcgagctt ggcggcggct tcctcccgct
     3841 gcggctcgac atgcgc
//
LOCUS       DASWNU010000250         6656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_60743, whole genome shotgun sequence.
ACCESSION   DASWNU010000250 DASWNU010000000
VERSION     DASWNU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60743"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VGB57_04930"
     CDS             <1..141
                     /locus_tag="VGB57_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system subunit TolQ"
                     /protein_id="HEY0130728.1"
                     /translation="LGLFAAIPAVIAYNRMTHGLNRLEAKLMRFADAFHGTLSRELEL
                     EG"
     gene            296..736
                     /locus_tag="VGB57_04935"
     CDS             296..736
                     /locus_tag="VGB57_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0130729.1"
                     /translation="MAMGPILGSHRRARRSPMAEINVTPMVDVMLVLLIIFMVTAPLL
                     VAGVPVDLPDSKAGALDQEQKPVQISLDSAGTLTVDDQPVAPADLGPRLAALAASSRE
                     PGGPRIFLRADRALDYGRVMAVMGEINAAGLRKVALVSTQGQER"
     gene            736..1572
                     /locus_tag="VGB57_04940"
     CDS             736..1572
                     /locus_tag="VGB57_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEY0130730.1"
                     /translation="MDRAEAAGLGVATAGHLALLAALSFGFAASRLPAPKSAPIEVSF
                     VDEVGLESTAPAPSAAEPAALLAPEEGPPEAAMPAPPQVQPIPQPQPAVRVPAPAPQP
                     TAKAASQPKPSPAPPKPAPKAAQAKPKPAPAKPAGAAKVAATRPTGRLSGLLAGVSDR
                     NTESRSTARPAAAAGPAVQASLGAEVRRQLKPHWKAPTGADSEQLRTEVIIALAQDGR
                     VTDIRIAGTTGQTASNRPQVPLHREQAERAVRLAAPFRLPAQYYEAWKSLRVTFDKRL
                     SQ"
     gene            1611..2885
                     /gene="tolB"
                     /locus_tag="VGB57_04945"
     CDS             1611..2885
                     /gene="tolB"
                     /locus_tag="VGB57_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY0130731.1"
                     /translation="MPAAAQLSVDVTDESGDDLVIAIPVMPTPQTTDTPAGSTEALGR
                     QVAEVVAADLRGSGLFRPVGPAGIRAVPFPEVTAPAYAAWSGAGAAALVQGFVRANGD
                     GQLTVGCYLYDVALQSELTRAGYVVAPRDWRRAAHKCADAIYSRLSGESPFFDSRIAY
                     IAESGPKNARVKRLAIMDSDGANHRFLTNGQSTVLTPRFSPDYKQIVYLSYVERRPRI
                     YIYDIGTGRQRIVTESANQTFAPRFSPDGRWILYAMSVGGNTDIYRVSALGGTPVRLT
                     NSPGIDVGGSYSPDGSRIVFESDRSGRQQLYVMNADGTGQRRISFGGGGYATPEWSPR
                     GDLIAFTRLAGNFRVGVMSPDGSGERLLTDSWQDEAPSWSPNGRVLQFFRTTQGRGGK
                     SSVWQVDLTGRNERRIPTPLDGSDPAWGPILP"
     gene            2908..3447
                     /gene="pal"
                     /locus_tag="VGB57_04950"
     CDS             2908..3447
                     /gene="pal"
                     /locus_tag="VGB57_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY0130732.1"
                     /translation="MRNSPWKVAAIAAALALTAGCGKKAPKDLPPPPGESADAGTATG
                     VDDESGDPSGGVGPGSREDFIRTVGLEGDRVFFDTDQYDLDDRDRATLDAQAAWLNRY
                     PAVRVTIEGHADERGTRDYNLALGDRRANAAKNYLAAGGVSAGRMTVISYGKERPEAL
                     GSDESAWARNRRAVTVLPQ"
     gene            complement(3587..4510)
                     /locus_tag="VGB57_04955"
     CDS             complement(3587..4510)
                     /locus_tag="VGB57_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY0130733.1"
                     /translation="MSQIPWKLVVHGGAGVIEREGSTPEQDRDIRASLDRALAAGAEV
                     LAEGGSALDAVERSARILEDDPQFNAGRGAVFTYQGTNELDAAVMDGRGRDAGAVTGV
                     TATRNPVSLARAVMEHSPHVFLSREGADQFSREHGLEQVGPEWFATEERRRQLDAMRA
                     EGDHYFDEELKYGTVGAVAVDIHGHVAAATSTGGLTGKRWGRIGDTPLIGAGTYADDR
                     SGAVSATGAGEYFIRAAVAAEICARMRMLGESAAVAADKVIADVGSLGGSGGIILVTP
                     EGDAAWAFNTPGMYRGIASAEGRRVAIYSDE"
     gene            4731..5642
                     /locus_tag="VGB57_04960"
     CDS             4731..5642
                     /locus_tag="VGB57_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0130734.1"
                     /translation="MTTTIHHSPDRQALRLSRERPAATFNGFVMLGVILLAIAAGLAA
                     LGLFAKSPVAALAIVAIAALVFVFVAAGFYLLQPNQAAALLLFGDYKGTDRTAGLRWR
                     WPWLTRTKISTRVHNVTSERLKVNDLRGNPIEIATNVVWRVTDTAQALFDVDDYTKFV
                     FVQIESAVRAIAAKYPYDDIEHQETTLRGHAEVVSQELKEELQERVGVAGITIDECRL
                     THLAYASEIAQSMLRRQQAEAVIAARQKIVIGAVSMVENALQLLSEKNVVELDDERRA
                     AMVSNLMVVLCSERDTQPIVNTGTLYQ"
     gene            5654..5860
                     /locus_tag="VGB57_04965"
     CDS             5654..5860
                     /locus_tag="VGB57_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEY0130735.1"
                     /translation="MPPPPKKAFPLRIEPALWAALERAAATDLRSVNAEIECLLREAL
                     QRRGVKLAPPEQRPRGRPPSEGKK"
     gene            5974..>6656
                     /locus_tag="VGB57_04970"
     CDS             5974..>6656
                     /locus_tag="VGB57_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0130736.1"
                     /translation="MAIINGTAGSDTIHGTNSSDTINGLGGDDTLFGNGGSDTLDGGT
                     GADSMSGGTGNDTYVVDDIGDVVNENSAEGTDTVRTTLAAYALTANVEHLRFIGAGDF
                     TGTGNDLNNDITGAGGNDTLSGGDGHDQLYGNGGDDSLYGGVGHDFLSGGSGADYLEG
                     NDGNDAYHVDNAGDVVAELSGEGTDEVYTTLTSYTLGSDVENLTSNTSSASAFHGIGN
                     GLGNILYGH"
BASE COUNT         1053 a         2424 c         2192 g          987 t
ORIGIN      
        1 ctcggcctgt tcgcggctat cccggcggtg attgcctaca accgaatgac ccacggcctg
       61 aaccgcctgg aagccaagct gatgcgcttt gccgacgcct ttcacggcac gctgagccgc
      121 gagctggagc tggaagggtg aggggttcgc attgtcttgc ctccccgccg cgaagcggtg
      181 gggaggggga ccacccgaag ggtggtggag gggtaactgg cgaggcctgc gccatctacc
      241 cctccaccat gcctcgcatg gtccccctcc ccatcttcga tggggaggca agctgatggc
      301 catgggtccg atcctcggct cgcaccgccg cgcccggcga tcgccgatgg cggagatcaa
      361 cgtcacgccg atggtcgacg tgatgctcgt cctgctcatc atcttcatgg tgacggcgcc
      421 gctcctcgtc gccggagtcc cggtcgacct gcccgacagc aaggcggggg cgctcgacca
      481 ggagcagaag cccgtccaga tctcgctcga ctccgccggc accctcaccg tcgacgacca
      541 gccggtcgcg cccgccgatc tcggcccccg cctcgccgcc ctcgccgctt cgtcgagaga
      601 gcccggcggc ccccgcatct tccttcgcgc cgaccgcgcc ctcgattacg gccgggtgat
      661 ggcggtgatg ggcgaaatca acgccgccgg cctgcgcaag gtcgccctcg tcagcacgca
      721 gggacaggag cggtgatgga ccgggcggaa gccgccggcc tcggagtcgc cacggcgggc
      781 catctcgccc tcctcgccgc gctcagcttc ggcttcgccg cgagccggct gccggcgccg
      841 aagtcggccc cgatcgaagt ctcgttcgtc gacgaagtgg gtttggaaag caccgccccg
      901 gcgccctcgg ccgccgagcc ggcggcgctc ctcgctccgg aagaggggcc gccggaggcc
      961 gcaatgccgg ccccgccgca agtgcagccg atcccgcagc cccagcccgc cgtccgggtg
     1021 cccgctccgg cgccccagcc gacggcgaaa gccgcctctc agccgaagcc gagcccggcg
     1081 ccgcccaagc cggcgccgaa ggccgcccag gcgaagccca agcctgcgcc cgccaaaccc
     1141 gccggggcgg ccaaagtggc cgcgacccgc ccgacggggc gcctgtcggg cctgctggcg
     1201 ggggtttcgg accgcaacac cgagagccgt tcaaccgcgc gtcccgccgc cgcggcgggc
     1261 ccggccgtgc aggcctcgct cggcgccgaa gtgcggcgcc agctcaagcc gcactggaag
     1321 gccccgaccg gcgccgattc cgagcagctg cgcaccgagg tcatcatcgc gctggcccag
     1381 gacgggcggg tgaccgacat ccggatcgcc ggcaccacag gccagacggc gagcaaccgc
     1441 ccccaggtcc cgctccaccg cgagcaggcc gagcgtgccg tccggctcgc cgcgcccttc
     1501 aggcttcccg cccaatatta cgaggcgtgg aagtcgctcc gggtcacatt cgacaagagg
     1561 ctctcgcaat gatgaagcgc ctgcttccgc ttttcctgtt cgcgttggcg atgccggccg
     1621 ccgcccaatt gagcgtcgac gtcaccgacg agagcggcga cgatctcgtc atcgcaatcc
     1681 ccgtcatgcc gactccgcag acgacggata cccccgccgg aagcaccgaa gcactcggcc
     1741 ggcaggtggc cgaagtcgtc gcggccgacc ttcgcggcag cggcctgttc cgtccggtcg
     1801 gccccgccgg catccgcgcc gtccccttcc cggaagtgac cgccccggct tatgccgcct
     1861 ggagcggggc cggcgccgcg gcgctcgtcc agggcttcgt ccgcgccaat ggcgacgggc
     1921 agctcaccgt cggctgctat ctctacgacg tcgcgcttca gtccgagttg acccgcgccg
     1981 gctatgtcgt cgccccgcgc gactggcgcc gcgccgctca taaatgcgcc gacgccatct
     2041 attcgcgcct gtcgggcgag agccccttct tcgacagccg gatcgcctac atcgccgaga
     2101 gcgggcccaa gaatgcgcgc gtcaagcggc tcgccatcat ggattcggac ggcgccaacc
     2161 accgcttcct gaccaacggc cagtcgaccg tcctgacccc tcgcttctcg cccgattaca
     2221 agcagatcgt ctatctcagc tatgtcgaga ggcggccccg gatctacatt tacgacatcg
     2281 gcaccggccg ccagcgcatc gtcaccgaga gcgccaacca gaccttcgcg ccccgtttct
     2341 cccccgacgg gcgctggatc ctctatgcca tgtcggtcgg cggcaatacc gacatctacc
     2401 gagtctccgc tttgggcggg actccggtcc gcctgaccaa ctcgccgggg atcgacgtcg
     2461 gcggctccta ttcgccagac ggcagccgga tcgtcttcga aagcgaccgc tcgggccgcc
     2521 agcagctcta cgtgatgaac gccgacggca ccggccagcg ccggatcagc ttcggcggcg
     2581 gcggctatgc gacccccgaa tggagcccgc gcggcgacct catcgccttc acccgcttgg
     2641 ccggcaattt ccgggtcggg gtgatgagcc cggacggatc gggcgagcgg ctcctgaccg
     2701 acagctggca ggacgaggcg ccgagctggt cgcccaacgg ccgggtcctc cagttcttcc
     2761 gaaccaccca ggggcgcggc ggcaagtcga gcgtctggca ggtcgacctc accggccgca
     2821 acgagcgccg aatccccacc ccgctggacg gttcggaccc cgcctggggc ccgatcctgc
     2881 cttgatgtat ccgataggag agagcgtatg agaaattccc cctggaaggt cgcggcgatc
     2941 gccgcggcgc tggcgctgac cgccggctgc ggcaagaagg cgcccaagga tctgccgccc
     3001 ccgccgggcg agagcgccga cgcgggcacc gccaccggcg tggacgacga gtcgggcgat
     3061 ccctcgggcg gggtgggtcc gggctctcgc gaggatttca tccgaacggt tgggctcgag
     3121 ggcgacaggg tcttcttcga taccgaccaa tatgatctcg acgaccgcga ccgggcgacg
     3181 ctcgacgccc aggcggcctg gctcaatcgc tatccggcgg tgcgggtcac catcgagggc
     3241 catgccgacg aacgcggcac ccgcgactac aatctcgccc tgggcgaccg ccgcgccaac
     3301 gccgccaaga actatctcgc cgcgggcgga gtctcggccg gccggatgac ggtgatcagc
     3361 tacggcaagg agcggccgga ggccctcggc tccgacgaaa gcgcctgggc ccggaaccga
     3421 agggcggtga cggtcctgcc gcaatgattt gcctccccat cgaagatggg gagggggacc
     3481 ccgcgtagcg gggtggaggg gtatttggcg caggcctgtt ccagctaccc ctccaccacc
     3541 cttcgggcgg tccccctccc cgccgctttc gcggcgggga ggcaagctat tcgtccgagt
     3601 agatcgccac ccttcgcccc tcggcggacg cgatgccgcg atacattccc ggcgtgttga
     3661 aggcccaggc ggcgtcgccc tcgggcgtga ccaggatgat ccccccgctt ccgcccagcg
     3721 agccgacgtc ggcgatcacc ttgtcggcgg cgaccgcggc gctttcgccg agcatccgca
     3781 ttcgggcgca gatttcggcg gcgacggcgg cccggatgaa atattcgccc gctccggtgg
     3841 cggagaccgc gccggagcgg tcgtcggcat aggtgccggc gccgatcagc ggcgtatcgc
     3901 cgatccggcc ccagcgcttg ccggtgaggc cgccggtgga ggtggcggcg gcgacgtggc
     3961 cgtgaatgtc gacggcgacg gcgccgaccg ttccgtattt cagctcctcg tcgaaataat
     4021 ggtcgccctc ggcccgcatc gcgtcgagct ggcgccgcct ctcctcggtc gcgaaccatt
     4081 cggggccgac ctgctcgagg ccgtgctcgc gcgagaattg gtccgcgcct tcgcggctga
     4141 ggaagacgtg ggggctgtgc tccatgaccg cccgggcgag gctgaccgga ttgcgggtgg
     4201 cggtgacccc ggtgacggcg ccggcgtcgc ggccgcggcc gtccatcacc gcggcgtcga
     4261 gctcgttcgt gccctggtag gtgaagacgg cgccgcgccc ggcgttgaac tggggatcgt
     4321 cctcgaggat ccgggccgaa cgctcgaccg cgtcgagggc gcttccgccc tcggccagca
     4381 cctcggcgcc cgcggccagc gcccggtcca gcgaggcccg gatgtcgcgg tcctgctcgg
     4441 gcgtagaccc ttcccgctcg atcacgcccg cgccgccgtg gacgacgagc ttccagggga
     4501 tttggctcat tcttacgctc cgttcgggag ggcaacgcat ggagggctcg ccctatccgc
     4561 aggggtgggg aggagcaagc agcgggtgaa gcgcgtcatt gcgaggagcg cagcgacgaa
     4621 gcaatccagc gggcctcgga ggccgctctg gattgcttcg cttcgctcgc aatgacggct
     4681 tgcatctccc tcatcatata attatgatat catcattaca ggagggaacg atgacgacta
     4741 ccatccacca ttctcccgat cgccaggcgc tccgcctctc gcgcgagcgg ccggccgcga
     4801 ccttcaacgg cttcgtcatg ctgggcgtga tactgctggc gatcgccgcc ggcctcgccg
     4861 ccctgggcct gttcgcgaag agccccgtcg ccgcgctggc gatcgtcgcc atcgccgcgc
     4921 tcgtcttcgt cttcgtcgcc gccggcttct acctgctcca gcccaaccag gcggcggcgc
     4981 tgctgctgtt cggcgactat aagggcaccg accgcaccgc cggcctcagg tggcgctggc
     5041 cgtggctgac ccggaccaag atctcgaccc gggtccacaa cgtcacttcg gagcggctca
     5101 aggtgaacga cctgcgcggc aacccgatcg agatcgccac caacgtcgtc tggcgggtca
     5161 ccgacaccgc ccaggccctg ttcgacgtcg acgattacac caagttcgtc ttcgtccaga
     5221 tcgagagcgc ggttcgcgcg atcgcggcca aatatcccta tgacgacatc gagcatcagg
     5281 agacgaccct tcgcggccat gccgaggtgg tcagccagga gctcaaggag gagcttcagg
     5341 agcgggtagg ggtcgccggg atcaccatcg acgaatgccg gctcacccac ctcgcctacg
     5401 cgtccgagat cgcccagtcg atgctccgcc gccagcaggc cgaggcggtg atcgcggccc
     5461 ggcagaagat cgtcatcggc gcggtctcga tggtcgagaa tgcgctgcaa ttgctgtccg
     5521 agaagaatgt cgtcgagctg gacgacgagc ggcgggcggc gatggtctcg aacctgatgg
     5581 tggtgctctg ctcggagcgc gacacccagc cgatcgtcaa caccggcacg ctctaccaat
     5641 aaggccgcag cgcatgccgc cgcccccgaa gaaggcgttc cccctgcgca tcgagccggc
     5701 cctgtgggcc gcgctcgagc gcgccgcggc aaccgatctg aggagcgtca acgcagagat
     5761 cgaatgcctt ttgcgcgagg cgctccagcg gcgcggggtg aagctggccc cgcccgagca
     5821 gcggccgcga ggccgcccgc cttcggaagg caagaagtag attggttcga ttcggatacg
     5881 attccccctt agggacagat ttcgtttcgc cacgggagta aagtcctgtc caccgcgccg
     5941 ccttccgcgg cgcctgggcg aaggggctct gaaatggcta ttatcaacgg cacggccggc
     6001 agcgatacga tccacggcac caacagttcg gacacgatca acggcttggg cggcgacgac
     6061 accctgttcg gcaacggcgg cagcgacacg ctcgacggcg gcaccggcgc cgattcgatg
     6121 agcgggggaa ccggcaacga cacctatgtc gtcgacgata tcggcgacgt tgtgaacgag
     6181 aattcggcgg aaggcaccga caccgtccga acgaccctcg ccgcctatgc gctcaccgcc
     6241 aatgtcgagc atctgcgctt catcggcgcc ggcgatttca ccggcaccgg caacgatctc
     6301 aacaacgaca ttaccggcgc cggcggcaac gacacgctct cgggcggcga cggccacgat
     6361 cagctctacg gcaatggcgg cgacgacagc ctctacggcg gcgttggcca cgatttcctc
     6421 tccggcggca gcggggccga ctatctggaa ggcaatgacg gcaacgacgc ctatcacgtc
     6481 gacaatgcag gcgacgtcgt cgccgagctg tcgggcgaag ggaccgacga ggtctacacc
     6541 acgctgacca gctacactct gggcagcgac gtcgagaacc tcacctccaa cacctcgtcc
     6601 gcctcggctt tccacggcat cggcaacggc ctcggcaaca tcctctacgg ccatag
//
LOCUS       DASWNU010000251         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_10118, whole genome shotgun sequence.
ACCESSION   DASWNU010000251 DASWNU010000000
VERSION     DASWNU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10118"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VGB57_04975"
     CDS             <1..212
                     /locus_tag="VGB57_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0130737.1"
                     /translation="EAMAAARIAPDVATRRGQLANADRILAGLGPFIPLTAPVRWSLV
                     APRLTGFRPNAFGRHPIGELILEQE"
     gene            222..659
                     /locus_tag="VGB57_04980"
     CDS             222..659
                     /locus_tag="VGB57_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY0130738.1"
                     /translation="MPTPQEQFDALAERLPVGRDPASVRRRIEAMEKLLERMFTLPGT
                     NRQFGLDVVFDLIPVGGSLIAAAMGSWLAWEARNLGMPKWHLARMGGNIGVDFLLGAI
                     PFVGAIPDFFFKSNTRNLRIVKRWLDRHHPETMIVEGEVVARR"
     gene            complement(726..1583)
                     /gene="nadC"
                     /locus_tag="VGB57_04985"
     CDS             complement(726..1583)
                     /gene="nadC"
                     /locus_tag="VGB57_04985"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0130739.1"
                     /translation="MTFSLPGFDLDRFVRETLAEDLGERGDITSEAVIPAEARFTGIM
                     DSRESIVAAGLPLAEAFFRALDPEVGIEPIVRDGDCVEAGADLLRLRGKARALLTAER
                     SALNIVQHLSGIATLTRRYVEAIAGTGAILLDTRKTIPGLRRLEKYATRMGGARNHRM
                     GLWDSAMIKDNHVAVAGGVAEAVRRAAAAGIERIIVEVDRLDQIEPALDGGATPLLLD
                     NMDAPVLREAVALVAGRVPTEASGGVTLESIRAKAETGVTYVSVGRITQSAPAADIGL
                     DFSPSLRGA"
     gene            complement(1719..>2757)
                     /locus_tag="VGB57_04990"
     CDS             complement(1719..>2757)
                     /locus_tag="VGB57_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0130740.1"
                     /translation="SGKLFALTPKKNGTWTSRSIALPANSTVHLLAVGGKPDLAFATV
                     EGMLTPPALYAVRPGGRPSLVQRLPAKFDAASRTVEQRFATSKDGTRVPYFLVRRKGA
                     DGPVPTLIHAYGGFRAAQTPGYLTGQPYRAGPLGMFWTEEGNAYVLANIRGGGEYGPE
                     WHKSVLRENRQKSFDDLHAVAEDLIRTGVTQKGKIGISGRSNGGVLVGAAMNQRPDLY
                     SAVINGSPLLDMKRYSHLLAGASWIDEYGDPDKPEDWAFLSRYSPYQNLKKGVRYPAV
                     FFYSSTKDDRVHPGHARKMAARLEEYGNRFYFHEYLEGGHSVGADNAEDAKRAALLMA
                     YLKRELAGAGR"
BASE COUNT          435 a          897 c          968 g          457 t
ORIGIN      
        1 acgaggcgat ggcggcggcg aggattgccc ccgacgtcgc cacccgccgc ggccagctcg
       61 ccaatgccga ccggatactt gccggcctcg ggccgttcat cccgctgacg gcgccggtgc
      121 gatggtcgct ggtcgcgccc cggctcaccg gattccgccc caacgccttc ggccgccacc
      181 cgatcggcga gctgatcctc gagcaagagt aggagtaacc gatgccgacg ccgcaggaac
      241 agttcgacgc gctcgcggaa cggctcccgg tcgggcgcga cccggcctcg gtccggcgcc
      301 ggatcgaggc gatggagaaa ttgctcgagc ggatgttcac cctgccgggc accaaccgtc
      361 agttcggcct cgacgtggtc ttcgacctga tcccggtcgg cggcagcctg atcgccgcgg
      421 cgatgggaag ctggctcgcc tgggaggcgc gcaaccttgg aatgccgaaa tggcatctgg
      481 cccggatggg cggcaatata ggggtcgact tcctgctcgg cgccataccc ttcgtcggcg
      541 ccatccccga ttttttcttc aaatcgaaca cgcgcaacct gaggatcgtc aagcgctggc
      601 tcgacaggca tcatcccgag acgatgatcg tcgagggcga ggtggtggcg cgccgctgac
      661 cgtcattgcg agcgcagcga agcaatccag gtgcggcctc ggaggccggc tggattgctt
      721 cgtcgctacg ctcctcgcaa tgacggggag aagtcgaggc cgatgtccgc cgcgggtgcg
      781 ctttgagtga tccggcccac cgagacgtag gtcacgcccg tctccgcctt ggcgcggatc
      841 gactccaggg tcacgccccc gctcgcctcc gtgggcaccc gccctgcgac cagcgccacc
      901 gcctcgcgaa gcaccggcgc gtccatattg tcgagcagca gaggggtggc gcccccgtcc
      961 agcgccggct cgatctggtc gagccggtcg acctcgacaa tgatccgctc gatgccggct
     1021 gccgccgccc ggcgcacggc ctcggcgacc ccgccggcaa cggcgacgtg gttgtccttg
     1081 atcatcgccg aatcccacag gcccatccgg tggttgcggg cgccgcccat ccgggtggcg
     1141 tatttctcga ggcggcggag gccggggatg gtcttgcggg tatccagcag gatcgcgccg
     1201 gtcccggcga tcgcctcgac gtagcggcgg gtgagggtgg cgatgccgga cagatgctgg
     1261 acgatgttga gcgccgagcg ctcggcggtg agcagcgccc gagccttgcc gcgcagccga
     1321 agcaggtcgg cgcccgcttc gacgcagtca ccgtcgcgga cgatcggctc gattccgact
     1381 tccggatcga gcgcccggaa aaaggcttcg gcgaggggca ggccggcggc gacgatcgac
     1441 tcgcgactgt ccataattcc ggtgaagcgc gcctcggcgg ggataacggc ctcggaagtg
     1501 atgtcgcccc gctcgccgag atcctcggcg agggtttcgc ggacgaagcg gtcgaggtcg
     1561 aagccgggga gggagaaggt catgtgcttg ggcctttccc gtcatgctga acttgtttca
     1621 gcatccagcg acgagccgcc gagtcgatgg agaactggac cctgaaacga gatcagggtg
     1681 acggcttgtg gcgacgactg cgcgtatcat gcaagcgtct accggcccgc cccggcaagc
     1741 tcccgcttga gataggccat cagcagggcc gcccgcttcg cgtcctcggc attgtcggct
     1801 ccgacgctgt ggccgccctc cagatattcg tggaagtaga agcggttgcc atattcctcc
     1861 agccgcgccg ccatcttgcg ggcgtggccg ggatggaccc ggtcgtcctt ggtcgaactg
     1921 tagaagaaga cggcggggta gcggacgccc ttcttcagat tctgataggg cgaatatctg
     1981 gacaggaagg cccagtcctc cggcttgtcg ggatctccat attcgtcgat ccacgaggct
     2041 ccggcgagga ggtgcgaata gcgcttcatg tcgagcagtg gggagccgtt gatgacggcg
     2101 gaatagagat cgggccgctg gttcatcgcc gccccgacca gcactcctcc gttggagcgt
     2161 ccggaaattc cgatcttgcc cttctgggtg actccggtgc ggatcaggtc ctcggcgacg
     2221 gcgtggagat cgtcgaagct cttctggcgg ttctcgcgga gcaccgactt gtgccattcg
     2281 ggcccgtatt cgccgccgcc gcggatgttg gcgaggacgt aggcattgcc ctcctccgtc
     2341 cagaacatcc cgagcggccc ggcgcgatag ggctggccgg tcaaatagcc gggagtctgg
     2401 gcggcgcgga agccgccata agcgtggatc agggtcggga cgggcccgtc ggcgcctttc
     2461 cggcgcacga ggaaataagg gaccctggtg ccgtccttcg acgtcgcgaa gcgctgctcg
     2521 acggtgcggc tggcggcgtc gaacttggcc ggcaggcgct ggaccaggga cggacggccg
     2581 ccgggccgga ccgcgtagag cgccggcggg gtcagcatgc cttccacggt ggcgaaggcg
     2641 aggtccggct tgccgccgac ggcgagcaaa tgaacggtgc tgttggccgg gagggcgatg
     2701 gagcgcgagg tccaggtccc gttcttcttc ggagtcaaag cgaacaattt gcccgag
//
LOCUS       DASWNU010000252         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_164149, whole genome shotgun sequence.
ACCESSION   DASWNU010000252 DASWNU010000000
VERSION     DASWNU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164149"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="serS"
                     /locus_tag="VGB57_04995"
     CDS             <1..983
                     /gene="serS"
                     /locus_tag="VGB57_04995"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0130741.1"
                     /translation="LEGLALRLPNIAWEGAPVGPDESFNIVVRTEGEPPGFGFEPRDH
                     VALVEMNDWADLSRITQVAGSRMYALKGRLALLEQALMFWAQRIIAEDGFTLMTVPAL
                     AKPQAFVATGHFPGHEEEAYEIEKDGLYLAGTGEIALTSLHSGEILDSAQLPILYAGY
                     SPCFRREAGSAGRDVRGLLRVHQFYKLEQYVICADDEEESARWHARLLANAERLLQGL
                     EIPYQVVETSTGDMGLGKFRMNDIESWVPSLGKYRETHSCSTLHDWQARRANLRWRDP
                     ERKVRFVHTLNNTALASPRILVPLLENHQEADGRVRLPRALQGLMGSEYL"
     gene            1018..1710
                     /locus_tag="VGB57_05000"
     CDS             1018..1710
                     /locus_tag="VGB57_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0130742.1"
                     /translation="MLEGTALLPRLYRAFGPLGQRGPADGARLMVLPGFLATDRTTLG
                     LQRALARASYRVSGWGLGLNRGVRPDILERIAARLEEFGGGAPVILVGWSLGGIYARE
                     VAKVRPDLVARVVTLGTPFSGDRRNNNVWRLYEAVARHPVDRPPVGGDPAVKPPVPTL
                     ALWSRRDGLIAPAAARGEPGERDLELELDCRHMGYAVSGRAFPKIVRAVTDFSAAGDG
                     GVAPLETPGLSR"
     gene            1813..3054
                     /locus_tag="VGB57_05005"
     CDS             1813..3054
                     /locus_tag="VGB57_05005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0130743.1"
                     /translation="MRILFVTWDGPQVAYLESLFLPIFAGLAEHGVHFDILQFRWGPE
                     AQAEAIARRCAAAGCGYRHSTIWRGLGGLGPFAAAFAGGREVRRAVRDFGSDAVMPRS
                     HMPGIAVAAAGGARLRPILFDADGFAVDEKVELGLLSASGPTYRILRRVEAGMVRASE
                     SVIVRSAAAARILRERAGGSIEPGRFHVVANGRDEAVFHPSDEGARRAVRAELGIDPA
                     VPLAVYAGSVGAQYRFDLIRDFAEALALQRPDARLLVLSGSPELAEAELGGTGSLAPI
                     VMRAPPDRVARYLAAADIGLAFRAQSFSVQGVAPVKLGEYLLCGVPAVGNVAVGDTGA
                     ALAAGVFLDDALGAAAAARWLVEEVMPRREDYRTRARAVGTERFSLRRSIDDYLAALA
                     PVANDRRPGAPLPVARAGTSL"
     gene            3137..3937
                     /locus_tag="VGB57_05010"
     CDS             3137..3937
                     /locus_tag="VGB57_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0130744.1"
                     /translation="MLPKETPVIILCGGEGTRIADVSRDAVPKPLIRIGDRPILWHIM
                     KIYAHFGYCNFILALGHLGWEIKNYFLNQRLMDSDFELVSRGGEVEINVHSLPQSNDW
                     RIVFAETGGKTQTGQRIALCRKYVQTDYFMATYGDGVADVDIEALARFAVEKGKVGTV
                     TSVTPSSRFGNIEVDEGGAVTAFAEKQDAGGGTINGGFFVFKREFMDIAERHGDVMLE
                     REPMDELVERSELAAYRHEGFWEPMDTMREFKQLNGMWDSGTAPWKMW"
     gene            3922..>4158
                     /locus_tag="VGB57_05015"
     CDS             3922..>4158
                     /locus_tag="VGB57_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEY0130745.1"
                     /translation="MEDVVTPSERLRSAYSGKRVLITGNTGFKGAWLALWLTRLGAKV
                     TGFSEDIATHPSHFERLGLDYRTIFGDIADAKAVR"
BASE COUNT          606 a         1436 c         1456 g          660 t
ORIGIN      
        1 agctcgaagg cctggccttg cggctgccca acatcgcctg ggaaggcgcg ccggtcgggc
       61 cggacgaaag cttcaacatc gtcgtccgga cggaggggga gccgccgggc ttcggcttcg
      121 agcctcggga ccatgtcgcc ctggtcgaga tgaacgactg ggcggatctc tcccgcatca
      181 cccaggtcgc cggctcgcgc atgtatgcgc tgaagggccg gctggcgctg ctcgagcagg
      241 cgctgatgtt ctgggcccag cggatcatcg ccgaggacgg cttcaccctg atgacggtgc
      301 cggccctggc caagccgcag gccttcgtcg ccaccgggca ttttccgggc cacgaggaag
      361 aggcctatga gatcgagaag gacggtctct acctcgccgg caccggcgag atcgctttga
      421 cctcgctcca ttccggcgag attctcgatt cggcgcagct gccgatcctc tatgccggct
      481 attccccctg cttccgccgc gaggcgggaa gcgccggccg cgacgtccgg gggctgctcc
      541 gggtccacca attctacaag ctcgagcaat atgtcatctg cgccgacgac gaggaggaat
      601 cggcgcgctg gcacgcccgg cttctcgcca atgccgagcg gctgctgcag gggctcgaaa
      661 tcccctatca ggtggtcgag acctcgaccg gcgacatggg cctcggcaag ttccggatga
      721 acgacatcga gagctgggtg ccgtccctgg gcaaatatcg cgagacccac agctgctcga
      781 cccttcacga ctggcaggcg cgccgcgcga acctgaggtg gcgcgacccg gagcgcaagg
      841 tgcgcttcgt ccacacgctc aacaacaccg cgctcgcgag cccccggatc ctcgtgccgc
      901 tgctcgagaa ccaccaggag gcggacgggc gggtgcggct gcccagggcg ctgcaggggc
      961 tgatggggag cgaatatctc tgacgccgat caccggccgc cgccgcggtg gcggctggtg
     1021 ctggaaggca cggccctgct cccgcgcctc tatcgcgctt tcggaccgct gggccagcgc
     1081 ggcccggcgg acggggccag gctgatggtc ctcccgggct tcctcgccac cgaccggacg
     1141 accctggggc tccagcgcgc gctggcccgg gccagctacc gggtgagcgg ctgggggctc
     1201 gggctgaatc gcggagtccg gccggacatc ctggaacgga tcgccgcccg gctggaggag
     1261 ttcggcggcg gggccccggt catcctggtc ggctggagcc tcggcggaat ctatgcgcgc
     1321 gaggtcgcca aggtgaggcc ggatctggtc gcgagggtcg tcacgctcgg cacgcctttc
     1381 tcgggcgatc gccgcaacaa caacgtctgg cgcctttacg aggccgtcgc ccgccatccg
     1441 gtcgatcggc cgcccgtcgg cggagacccc gcggtcaagc cgccggtgcc gaccctcgcc
     1501 ttatggtcgc gacgcgacgg cctcatcgcc ccggccgccg cccgcggcga gccgggggag
     1561 cgcgacctcg agctggagct cgactgccgc cacatgggct atgcggtcag cggccgcgcc
     1621 tttccgaaga tcgtccgggc cgtcaccgac ttctcggcgg ccggggatgg aggggttgcg
     1681 ccgctggaaa ccccggggct tagccgctag tcggcgaatt cggcccggct cccacggccg
     1741 gtgccgggcg cggctcccgc tcctccggca ttggcggact tgaggggggc ggtcgatcga
     1801 aaggtgtcgt cgttgagaat cctgttcgtc acttgggacg gccctcaggt cgcttatctt
     1861 gaatcgctgt tcctgccgat attcgccggg ctggccgagc acggcgtcca tttcgacatc
     1921 cttcagttcc gctggggtcc ggaggcccag gccgaggcga tcgccaggcg ctgcgcggcg
     1981 gcgggctgcg gctaccggca ctcgacgatc tggcgcggcc tcggcggcct cggcccgttt
     2041 gccgcggcct tcgcgggggg gcgcgaggtc cgccgggccg tccgcgactt cggcagcgac
     2101 gccgtcatgc cgcgcagcca catgccgggc atcgcggtgg ccgccgccgg gggcgcgcgc
     2161 ctcaggccga tcctgttcga cgccgacggc ttcgccgtcg acgagaaagt ggagctcggc
     2221 ctgctgtccg ccagcggccc gacctatcgt atcctccgaa gggtcgaggc ggggatggtt
     2281 cgcgcctcgg aatcggtcat cgtccgctcc gcggctgcgg cgcggatcct gcgcgagcgg
     2341 gccggcggga gcattgagcc cgggcgtttc catgtcgtgg cgaacggccg cgacgaggcc
     2401 gtcttccacc cgtcggacga gggcgcgcgg agggcggttc gagccgagct cgggatcgac
     2461 cccgcggtcc cgctggcggt ctacgccggc tcggtcggag cgcaatatcg cttcgacctc
     2521 atccgcgact tcgccgaagc gcttgccctc cagcgccccg acgcccgcct gctggtgctg
     2581 agcgggtcgc ctgagctcgc cgaggccgag ctcggcggga ccggctcgct tgctccgatc
     2641 gtcatgcgcg cgccgccgga ccgggtcgcg cgctatctgg cggcggcgga catcggcctc
     2701 gccttccgcg cccagtcctt ctcggtccag ggagtcgcgc cggtgaagct cggcgaatat
     2761 ctgctgtgcg gcgtacccgc cgtgggcaac gtggcggtgg gcgacaccgg ggcggcgctc
     2821 gccgccggag tcttcctcga cgacgcgctc ggcgcggcgg ccgcggcgcg gtggctggtc
     2881 gaagaggtca tgccccggcg cgaggattac cggacgcgcg ctcgggcggt cggcaccgag
     2941 cgattctccc tccggcgaag catcgacgac tatctggccg cccttgcccc ggtcgcgaac
     3001 gaccggaggc cgggggcccc ccttcctgtt gcgcgggctg gcaccagcct ctaaagcctg
     3061 cccgccgtca ggggccgctc ggcgggcggg gcgaatactc gaccgatttc cggacatttc
     3121 gaaagccgtt cgcaagatgc tacccaaaga gacaccggtc atcatcctgt gcggaggcga
     3181 aggcacgcgg atcgccgatg tctcgcgcga cgcggtgccg aagcctttga tccgcatcgg
     3241 cgaccggccg atcctgtggc acatcatgaa gatctatgcc catttcggct attgcaactt
     3301 catcctggcg ctcgggcacc tcggctggga gatcaagaac tacttcctca atcagcggct
     3361 gatggattcc gatttcgagc tggtgagccg cggcggcgag gtggagatca acgtccacag
     3421 cctgccccag tccaacgact ggcggatcgt cttcgccgag accggcggca agacccagac
     3481 ggggcagcgc atcgccctgt gccgcaaata tgtccagacc gactatttca tggcgaccta
     3541 tggcgacgga gtcgccgacg tcgacataga ggccctagcg cgcttcgccg tcgaaaaggg
     3601 caaagtgggc accgtcacca gcgtcactcc gtcgagccgg ttcggcaaca tcgaagtgga
     3661 cgagggcggc gccgtgaccg ccttcgccga gaagcaggat gccgggggcg gcaccatcaa
     3721 cggcggcttc ttcgtcttca agcgcgagtt catggacatc gccgagcgcc atggcgacgt
     3781 catgctggaa cgcgagccga tggacgagct ggttgagcgc tcggagctcg ccgcctatcg
     3841 ccacgaaggc ttctgggagc cgatggacac gatgcgcgag ttcaagcagc tcaacggaat
     3901 gtgggacagc gggaccgcgc catggaagat gtggtgactc cgtccgagag gctgcgctcg
     3961 gcttattccg gcaagcgggt cctcatcacc ggcaataccg ggttcaaggg cgcctggctc
     4021 gcgctctggc tcacccgcct cggggcgaag gtgacgggct tctccgagga tatcgccacc
     4081 catcccagtc atttcgagcg ccttggcctc gattaccgaa cgatcttcgg cgatatcgcc
     4141 gacgccaagg cggtccgc
//
LOCUS       DASWNU010000253         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_87202, whole genome shotgun sequence.
ACCESSION   DASWNU010000253 DASWNU010000000
VERSION     DASWNU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87202"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VGB57_05020"
     CDS             <1..396
                     /locus_tag="VGB57_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0130746.1"
                     /translation="EAERSDLKDGLEGLARSVPRPVIHLDISPDVRVGPAQAHALIRC
                     AQEAVTNAVRHAEASNLWLEVTPDGEGVRLVARNDGRARPAASSPGSGLVGMRERVES
                     LGGRLAVRKGPDFGFTLDAWLPSRGPQAA"
     gene            393..1079
                     /locus_tag="VGB57_05025"
     CDS             393..1079
                     /locus_tag="VGB57_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0130747.1"
                     /translation="MIGPDESGQDDPARMIRVVLVDDQTLVRQGVRGLLELVPDIEVV
                     GEASDGEEALEIVPGLKPDVLLLDIRMPRLSGIAVLEALSEAKALPPTLVLTTFDDGD
                     AAIAAIKAGARGLMLKDVSLEDLAQAIRALADNKTAFQPAMTDSLMAAIRRSPSASTD
                     TYVAESLTVREREVLHLICAGYSNREIADLLALAEGTVKNHVSNLLLKLDARDRTRAA
                     LKALQQGYLS"
     gene            complement(1162..2118)
                     /locus_tag="VGB57_05030"
     CDS             complement(1162..2118)
                     /locus_tag="VGB57_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130748.1"
                     /translation="MARPLTPLQAHENQRFLERFSLSGNVRLAAREAGVKYGTIQHRR
                     RKHAAFAQAWEAAAAAVQARLHLAGGKRGPEPAARRTRNPLRTKGGEPMVVRTRNGKL
                     QIRLAHPDKLTKAAEQAFLYALSATANVRLSAAAAGASVAAFYRRRRQNPAFAREMRL
                     ALRMGWQRLEMAAMQASTPESHADDQWRNCDLAPMPQFTANQAVQLLCLHDKSVNQGW
                     EEPHRRRRRGESDEIYSLRLQAMWRVERSLEAEAEAVRRAFRFEETGGWRHEDEVPPP
                     ELVPLELVTGWSKASGRPAHHEGLALFGGWRIADMEKRLGRG"
BASE COUNT          320 a          831 c          746 g          342 t
ORIGIN      
        1 gaggccgagc gctccgatct gaaggacggg ctcgaggggc ttgcgcgaag cgtgccgagg
       61 cccgtcatcc atctcgacat ctcccccgac gtccgggtcg gccccgccca ggcccacgcc
      121 ctgatccgat gcgcgcagga agccgtgacc aacgccgtca ggcacgcgga agcctccaac
      181 ctctggctcg aggtgacccc ggacggcgaa ggcgtgcgcc tcgtcgcgcg caacgacggc
      241 cgggcgcgtc ccgccgcttc ctcgcccggc tcggggcttg tcgggatgcg cgaacgggtg
      301 gagtcgctgg gcggcaggct ggcggttcgg aagggccccg atttcggctt cacgctcgac
      361 gcctggctgc cttcgcgcgg gccgcaagcc gcatgatcgg gcccgatgaa tccggccagg
      421 atgatccggc caggatgatc agggtggttc ttgtcgacga tcagaccctg gtccgccagg
      481 gcgttcgcgg cctgctcgag ctggtgcccg acatcgaggt ggtgggcgag gcgagcgacg
      541 gcgaggaagc gctcgagata gtgccagggc tcaagcccga cgtgctcctc ctcgacataa
      601 ggatgccccg gctgagcggc atcgccgtcc tcgaggcgct gagcgaggcg aaggcgcttc
      661 cgcccaccct ggtcctgacc accttcgacg acggcgatgc cgcgatcgcc gcgatcaagg
      721 ccggcgccag gggcctgatg ctcaaggacg tctcgctcga ggacctggcc caggcgatcc
      781 gggcgctggc cgacaacaag acggcgttcc agcccgccat gaccgacagc ctgatggcgg
      841 cgatccgccg aagcccttcg gcttcgaccg acacctatgt cgccgagtcg ctcaccgtcc
      901 gggagcgcga ggtgcttcac ctgatctgcg ccggctacag caacagggag atcgccgatc
      961 tcctggcgct ggcggagggg acggtgaaga accacgtctc caacctcctc ctcaagctcg
     1021 acgcccgcga ccgcacccgg gccgccctca aggccctcca gcaggggtat ctaagctgaa
     1081 gcgcccgagc gcgctggatt tactcccgtg acggctccag catgggaatc cgggtgccgc
     1141 attggatggc cgcccggccg ctcagcccct gcccagacgc ttctccatat ccgcgatccg
     1201 ccagccgccg aacagggcca ggccctcatg gtgcgccggc cgcccgctcg ccttcgacca
     1261 ccccgtcacc agctcgagcg gcacgagctc gggcggcggc acttcgtcct cgtggcgcca
     1321 tccgcccgtc tcctcgaagc ggaaggcgcg cctcaccgcc tccgcctcgg cctccaggct
     1381 cctctcgacc ctccacatcg cctgcagccg cagcgaatag atctcgtcgc tctcgccgcg
     1441 ccgccgccgc cgatggggct cctcccagcc ctggttgacg ctcttgtcat ggaggcagag
     1501 gagctggacc gcctggttcg cggtgaattg cggcatcggc gcgaggtcgc aattgcgcca
     1561 ctggtcgtcg gcatggctct ccggggtcga agcctgcatc gccgccatct cgagccgctg
     1621 ccagcccatc ctgagcgcca gccgcatctc ccgggcgaag gccgggttct gccgccgccg
     1681 ccgatagaaa gcggcgaccg aggccccggc ggcggcggcg ctcagccgca cgttggcggt
     1741 ggcggacaaa gcgtagagga aggcctgctc ggccgccttg gtcagcttgt ccggatgggc
     1801 gagccggatc tggagcttgc cgttccgggt gcgcaccacc atcggctccc cgcccttggt
     1861 acgcagggga ttgcgggtcc gccgggcggc gggctccggc ccccgcttgc ccccggcgag
     1921 gtgaagccgc gcctgcaccg cggcggcggc cgcctcccac gcctgcgcga aagcggcatg
     1981 cttcctgcgc cggtgctgga tcgtcccgta cttcaccccc gcctcccgag ccgccagccg
     2041 gacgttcccg ctcagcgaga accgttcgag gaagcgctgg ttctcatgcg cctggagcgg
     2101 ggtgagggga cgggccatgg cattcctccg attcgcctgt tcccatcatg ttcccgcaag
     2161 ccgccgcgca ttgaaagggt gcaacttcgg gcgctttcgc ttcctccagt cgtcaccctg
     2221 aacccgtttc agggtccag
//
LOCUS       DASWNU010000254         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_113800, whole genome shotgun sequence.
ACCESSION   DASWNU010000254 DASWNU010000000
VERSION     DASWNU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113800"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1994
                     /locus_tag="VGB57_05035"
     CDS             <1..>1994
                     /locus_tag="VGB57_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY0130749.1"
                     /translation="GIAHIRGKSDADAVFGMIYAQAEDDFNRVETNYLTALGRTAEAE
                     GEKAIWADLRARLYMDPDDLRARYAASPAWLKRLMDAWADGLNYYLATHPDVRPRAIA
                     RFEPWMALSFTEGSIGGDIERIDLAALEAFYSKRGRALASAAPAYDPEPRGSNGFAIA
                     PSNSSGGHALLLINPHTSFFFRSEQQVASDEGLNAYGAATWGQFFIYQGFNDRSGWMH
                     TSSGVDVVDEFLETVEQKQDGLFYRYGNALRPVATKPVTVAWRQADGSMASRSFETYH
                     THHGPIVREAGGKWVAFAMMHKPVEALQQSFLRTKVRDHEGFLKVAELKANSSNNTVF
                     ADAEGNIAYHHPHFIPVRDDRFDYTRPVDGSDPRTDWRGVHALSEAPRVLNPPNGWIQ
                     NTNNWPYSAAGPHSPKPADFPRYMDTFGENHRGLAAMRVLQEKTQLTLEGLRDAAYDP
                     YQPAFAELVPGLVEAYEKLAPGHRLKGKLMRPTDFLRRWDYRWSEDSVATTLAMFWAE
                     PLYDEARKAGRPLGETVSSIGRLSDRRKLEALEQAMDRLRGEFGRWNVEWGEVNRFQR
                     LTGDIVHPFSDAAPSLPVAFTSGRWGSLASFGAAPRNGSKRWYGTSGNSFVAVVEFGP
                     KVRARAVTAGGESGHPGSPHFNDQAERYAKGDLREVYFHP"
BASE COUNT          341 a          654 c          697 g          302 t
ORIGIN      
        1 gggcatcgcc cacatccgcg gcaagagcga tgccgacgcg gtgttcggca tgatctacgc
       61 gcaggccgag gacgacttca accgggtcga gaccaactat ctcaccgccc tcggccgaac
      121 cgccgaggcg gagggagaga aggcgatctg ggccgatctg cgcgccaggc tctacatgga
      181 cccggatgat ctcagggccc gctacgcggc cagcccggcc tggctgaagc ggctgatgga
      241 tgcctgggcg gacggcctga attattatct ggcgacccat cccgacgttc gtccgcgcgc
      301 gatcgcccgg ttcgagccgt ggatggcgct gagcttcacc gaaggcagca tcggcggcga
      361 catcgagcgg atcgaccttg cggcgctgga ggcgttctac tcgaagcgcg gacgggcgct
      421 cgcctcggcc gcgcccgcct acgatccgga gccccgaggc tcgaacggct tcgccatcgc
      481 gccttcgaat agctcgggcg ggcacgccct gctcctgatc aacccgcaca ccagcttctt
      541 cttccgctcc gagcaacagg tggcgagcga cgaggggctg aacgcttacg gggcggcgac
      601 ctggggacag ttcttcatct accagggctt caacgaccgc tcgggctgga tgcacacgtc
      661 gagcggagtc gatgtcgtcg acgaattcct cgagacggtg gagcagaagc aggacggcct
      721 cttctaccgc tacggcaacg cgctgaggcc ggtggcgacg aagccggtga ccgtcgcctg
      781 gcgccaggcg gacgggagca tggccagccg aagcttcgag acctaccaca cccatcacgg
      841 gccgatcgtc cgcgaggcgg gcggcaaatg ggtcgccttc gcgatgatgc acaagccggt
      901 cgaggcgctt cagcagagct tcctgcggac gaaggtgcgc gaccacgagg gcttcctgaa
      961 ggtagcggag ctcaaggcca attcgtcgaa caacaccgtc ttcgccgacg ccgagggcaa
     1021 catcgcctat caccatccgc acttcattcc ggtgcgcgac gaccgcttcg attataccag
     1081 gccggtggac ggcagcgacc cgcggaccga ctggcggggc gtgcacgcgc tgagcgaggc
     1141 gccgcgcgtg ctcaacccgc ccaacgggtg gatccagaac accaacaact ggccctattc
     1201 ggccgccggg ccgcacagcc cgaagcccgc cgatttcccg cgttacatgg acacgttcgg
     1261 cgagaatcac cgcggcctcg cggcgatgcg ggtgcttcag gagaaaacgc agctgaccct
     1321 ggaggggctg cgcgacgccg cctacgaccc ctatcagccg gctttcgccg agctggttcc
     1381 cggcctcgtc gaggcatatg agaagcttgc cccgggccat cggctgaagg ggaagctcat
     1441 gcgcccaact gatttcctca ggcggtggga ctatcgctgg agcgaggatt ccgtcgcgac
     1501 gacgctggcg atgttctggg ccgagccgct ctacgatgag gcgcgcaagg cgggacggcc
     1561 gctcggggag acggtgtcga gcatcggcag gctgtccgac cggcgcaagc tcgaggcgct
     1621 cgagcaggcg atggaccgtc ttcgcggtga gttcggccgg tggaacgtgg agtggggcga
     1681 ggtgaaccgc ttccagcgcc tgacgggcga tatcgtccat cccttctccg atgccgcgcc
     1741 gagccttccg gtggcgttca cctcgggccg ctggggatcg ctcgcttcct tcggggcggc
     1801 gccgaggaac ggctcgaagc gctggtacgg gacgagcggc aacagcttcg tggcggtggt
     1861 ggagttcggg ccgaaggtgc gggcgcgggc ggtgacggcg ggcggcgaga gcggccatcc
     1921 cgggtcgccg cacttcaacg accaggcgga acgctatgcg aagggggact tgcgcgaggt
     1981 ctatttccat cccg
//
LOCUS       DASWNU010000255         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_294699, whole genome shotgun sequence.
ACCESSION   DASWNU010000255 DASWNU010000000
VERSION     DASWNU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294699"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /gene="hemW"
                     /locus_tag="VGB57_05040"
     CDS             complement(<1..1064)
                     /gene="hemW"
                     /locus_tag="VGB57_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY0130750.1"
                     /translation="MLRCARNDGASPGLALYVHWPFCVSKCPYCDFNSHVRESIDQAQ
                     WRDALLLDLAWEAEAFPGRGLSSIFFGGGTPSLMEPATVAALIEAADRHWGLAPDVEI
                     TLEANPSSVEAERFADLASAGVNRVSLGLQSLDDRALRFLGRAHDCAEGQSALATAQS
                     CFERVSFDLIYALPGQSEADWAAELGRALGFGTGHLSLYQLTIEPGTRFAAMAARGEL
                     DARDPDEAAALYELTQAMTEQAGLPAYEISNHARPGEESRHNLAYWRYRPYLGVGPGA
                     HGRRGGNATQRHRKPENWLAALRRNGHGLAEETFLGPEEQATEALLMGLRLSEGVELE
                     AVEERLDRGAVERLEGHGLL"
     gene            complement(1148..1795)
                     /gene="rdgB"
                     /locus_tag="VGB57_05045"
     CDS             complement(1148..1795)
                     /gene="rdgB"
                     /locus_tag="VGB57_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY0130751.1"
                     /translation="MRKLQPGRLVIASHNEGKVREIRELLGPYGVDPVSAAELDLPEP
                     DEIGTTFIDNADLKAREAADLSGLPALADDSGLCVEALEGRPGIFSARWALAADPRVA
                     PAAGPGEVEGPRDFGLAMRRVWEELEALGPEASRNAHFVCALAVCWPDGRSEWFEGRV
                     EGTLVWPPRGDRGFGYDPIFVAAGRDVTFGEMDPAEKHRISHRADAFRKLVAALF"
     gene            complement(1792..>2241)
                     /gene="rph"
                     /locus_tag="VGB57_05050"
     CDS             complement(1792..>2241)
                     /gene="rph"
                     /locus_tag="VGB57_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0130752.1"
                     /translation="IQRLIGRSLRAVTDLKALGERQIQVDCDVVQADGGTRTAAISGG
                     WVALRIAVDGLLRQQLLTADPIRQKIAAVSCGIHNGAAVLDLDYEEDSNAGADGNFVL
                     TGDGNIVEAQVTAEGETYDEEGLLRLLRLARIGCGQIFAAQDRATGR"
BASE COUNT          324 a          827 c          754 g          336 t
ORIGIN      
        1 tcgagcagcc catggccctc cagccgctcg accgcgcccc ggtcgagccg ctcttccacc
       61 gcctccagct ccaccccctc gctgagccga agccccatca gcagcgcctc cgtggcctgc
      121 tcctccgggc ccaggaaagt ctcctcggcg agcccgtggc cgttgcgcct cagcgccgcc
      181 agccagttct ccggcttgcg gtggcgctgg gtggcgttgc ccccgcgccg cccgtgcgcc
      241 ccggggccta ctccgagata gggccggtaa cgccagtaag cgagattgtg gcggctctcc
      301 tcgcccgggc gggcgtggtt cgagatctcg taggcgggca gcccggcctg ctccgtcatc
      361 gcctgggtca gctcatagag ggccgccgct tcgtcgggat cgcgcgcatc gagctcgccc
      421 cgcgccgcca tggccgcgaa gcgcgtgccg ggctcgatgg tcagctgata gagcgagaga
      481 tggccggtgc cgaagccgag cgcccgaccg agctccgccg cccagtccgc ctcgctctgg
      541 cccggcaaag cgtagattag gtcgaagctg acccgttcga agcagctttg cgccgtcgcc
      601 agcgcgcttt gcccttccgc gcaatcatgg gcccggccga ggaagcgaag cgcccggtcg
      661 tcgagcgact ggaggccgag cgacacccgg ttcacgcctg ccgaggcgag gtcggcgaag
      721 cgctccgcct cgaccgagga ggggttggcc tcgagcgtga tctcgacgtc cggcgccagc
      781 ccccaatgcc tgtccgccgc ctcgatcagg gccgccaccg tcgccggctc catcagcgag
      841 ggcgtgccgc cgccgaagaa gatcgaggaa aggccgcgtc cgggaaaggc ctccgcttcc
      901 caggcgagat cgagcagcag ggcatcgcgc cattgcgctt ggtcgatcga ctcgcggaca
      961 tggctgttga agtcgcaata agggcatttg gagacgcaga agggccagtg gacgtagagg
     1021 gcgaggccgg gggatgctcc gtcattgcga gcgcagcgaa gcaatccagc ctccggtgcc
     1081 gacgccggcg ccgggctgga ttgcttcgtc gctgcgctcc tcgcaatgac ggtgctccca
     1141 cccattccta aaacagcgcc gccaccagct tgcgaaacgc gtccgccctg tggctgatgc
     1201 ggtgcttctc ggccgggtcc atctcgccga aggtgacgtc gcggccggcg gcaacgaaga
     1261 tcggatcgta gccgaagcct ctgtctcccc gcggcggcca gaccagcgtc ccctcgaccc
     1321 ttccttcgaa ccattcgctg cgcccgtcgg gccagcagac cgcgagggcg cagacgaaat
     1381 gggcgttgcg gctcgcttcc gggccgagcg cctccaattc ctcccacacc cggcgcatcg
     1441 ccagcccgaa gtcgcgcggc ccctcgacct cgcccggccc ggcggccggg gcaacgcgcg
     1501 ggtcggcggc cagcgcccag cgcgccgaga agattccggg cctcccctcg agcgcctcga
     1561 cgcaaagacc gctgtcgtcg gcgagcgcgg gcaggccgga caggtccgcc gcctcccgcg
     1621 ccttcagatc cgcattgtcg atgaaggtgg ttccgatctc gtccggctcg ggaaggtcga
     1681 gctcggcggc ggagaccgga tccacgccat agggcccgag cagctcgcga atctcgcgca
     1741 ccttgccttc gttatggctg gcgatgacca gcctgcccgg ctgaagcttc ctcaccgccc
     1801 cgtcgcccgg tcctgggccg cgaagatctg gccgcagccg atccgggcga ggcggagcag
     1861 gcggagcagg ccttcctcgt cataggtctc gccctcggcc gtgacctgcg cctcgacgat
     1921 gttgccgtcg ccggtgagga cgaaattgcc gtccgcgccg gcgttggaat cctcctcgta
     1981 atcgaggtcg agcaccgcgg cgccattatg gattccgcag ctcaccgcgg cgatcttctg
     2041 ccggatcgga tcggcggtga ggagctgctg gcggagaagt ccgtcgaccg cgatcctcag
     2101 cgccacccag ccgcccgaaa tggccgcggt gcgggtgccg ccgtcggcct ggacgacgtc
     2161 gcagtcgacc tggatctggc gttcgcccag cgccttcagg tcggtgaccg cgcgcagcga
     2221 ccgcccgatc agccgctgga t
//
LOCUS       DASWNU010000256         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_138501, whole genome shotgun sequence.
ACCESSION   DASWNU010000256 DASWNU010000000
VERSION     DASWNU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138501"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1188
                     /gene="murG"
                     /locus_tag="VGB57_05055"
     CDS             28..1188
                     /gene="murG"
                     /locus_tag="VGB57_05055"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY0130753.1"
                     /translation="MLSRHYVLAAGGTGGHMIPAHALAEELMRRGHRVALVTDDRGAR
                     IPGLFEGVPVHILPAGRLGGGPMGWLSAIRKIFTGRAMASRLYETFEPSAVIGFGGYP
                     ALPALMAAQRDRIPTLIHEQNAVLGRVNRIVSGRVDAIATAYPNVQRLAKSREAKTRL
                     VGNPVRDEVLALREQPYPELTDEGVFRLLVTGGSQGATILSQVVPEALGLLPEHFRRR
                     LQVTQQCRAEDIESVRARYSALGIAAELATYIDDMPDKIAWTHLVIARAGASTIAELT
                     VAGRPAILVPLPSATDDHQTFNAREMARAGGARTISQRNFTPVELAKQMQKLGLEPAA
                     LANAASRAWAVGRPNAASDLADLVEMTGRDLGADPIPAEEMILTPLPSGAFA"
     gene            1203..2615
                     /gene="murC"
                     /locus_tag="VGB57_05060"
     CDS             1203..2615
                     /gene="murC"
                     /locus_tag="VGB57_05060"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY0130754.1"
                     /translation="MRGVAKDIGTIHFVGIGGIGMSGIAEVMHILGYKVQGSDVAEGY
                     VVEGLRKAGIPVKVGHHPDNLGDSAVVVISTAIKKDNPELVCAYERRIPVVRRAEMLA
                     ELMRLKSTVAVAGTHGKTTTTSMIAAMLDAGGIDPTVINGGIINAYGSNARLGSSDWM
                     VVEADESDGSFLRLDGTLAVVTNIDPEHLDHYGSFDKVKDAFVEFVENVPFYGAALLC
                     VDHPEVQTIIPRLRDRRVVTYGFAALADVRGENVRPVPGGNLFDVSLRDRSGDVATIA
                     DIELPMPGRHNVQNALAAIGIAAELGIPHSTIARGFEKFGGVKRRFTKVGEIEGATII
                     DDYGHHPTEIRAVLSAAREGAQGRVIAVVQPHRYTRLRDLMEEFQGAFNDADVVYVTP
                     VYPAGEEPIEGVDSEALAEGLKQRGHRAVKAVADLDELCLGLRDLAASGDMVICLGAG
                     DITKWAAALADGIACARRDK"
     gene            2612..3526
                     /gene="murB"
                     /locus_tag="VGB57_05065"
     CDS             2612..3526
                     /gene="murB"
                     /locus_tag="VGB57_05065"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY0130755.1"
                     /translation="MSGLRHVETWGIPSLEGVAEANGSLADFIWFRTGGAAEWLVRPK
                     DVEDLARFLRELDPSMPVLPVGVGSNLIVRDGGVPGVVVRLPKAMSKVNIEPGNRVRA
                     GGGAMGITVASKARDADIAGLEFLRGIPGTAGGAVRMNAGAYGRESADILVEATLVLR
                     DGRIETWPAERLGYTYRHSEVPAGAVVVEAVFQGVPGERSAIGAEMDRIAAEREASQP
                     LRSRTGGSTFKNPPGTRAWKLIDEAGCRGLTIGDAQVSEKHCNFLLNLGKARSSDIEA
                     LGEEVRRRVLEHSGVLLEWEIQRVGVAG"
     gene            3615..>4098
                     /locus_tag="VGB57_05070"
     CDS             3615..>4098
                     /locus_tag="VGB57_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0130756.1"
                     /translation="MVTSPLHVAVLMGGWSAEREVSLMSGRDIASALESLGHRVTRID
                     MDRNVAQRLSEIAPDVVFNALHGTPGEDGAVQGMMDLMGLKYTHSGLETSVIAIDKEL
                     TKMVLVPHGIRMPEGQIVDSESLYEGDPMSRPYVLKPVNEGSSVGVAIVTDQGNCGKP
                     I"
BASE COUNT          673 a         1395 c         1410 g          620 t
ORIGIN      
        1 tcccctgcaa atgcagggga ggatttcatg ctgagtcgcc actacgtcct cgccgccggc
       61 ggcaccggcg ggcacatgat ccccgcccac gctttggccg aggagctgat gcggcgcggg
      121 catcgcgtgg cgctcgtcac cgacgatcgc ggcgcccgaa tccccggcct gttcgaaggc
      181 gtgcccgtcc acatccttcc cgccggccgg ctcggcggcg ggccgatggg ctggctgagc
      241 gcgatccgca agatcttcac cggccgggcg atggcgtcgc ggctctacga gacgttcgag
      301 ccttcggcgg tgatcggctt cggcggctat ccggcgcttc cggccttgat ggcggctcag
      361 cgcgaccgga tcccgaccct gatccacgag cagaatgcgg tgctcggccg ggtcaaccgg
      421 atcgtctcgg gccgggtcga cgcgatcgcc accgcttacc ccaacgtcca gcggctcgcc
      481 aaaagccgcg aggccaagac ccgtctcgtc ggcaaccccg tgcgcgacga ggtgctggcg
      541 ctgcgcgaac agccttatcc cgagctcacc gacgaaggcg tgttccggct tctcgtcacc
      601 ggcggaagcc agggcgcgac catcctgtcg caggtggtgc ccgaagccct cggcctcctg
      661 cccgagcact tccgccgccg cctccaggtc acccagcaat gccgggccga ggatatcgag
      721 agcgtccggg cccgctactc ggcgctgggg atcgccgccg agctcgccac ctacatcgac
      781 gacatgcccg acaagatcgc ctggacgcac ctcgtcatcg cccgcgccgg agcctcgacc
      841 atcgccgagc tgacggtggc gggccggccg gcgatcctcg tcccgctgcc ctcggcgacc
      901 gacgaccacc agaccttcaa cgcccgcgag atggccaggg ccggaggggc gcggacgatc
      961 agccagagga acttcactcc cgtcgagctc gccaagcaga tgcagaagct gggcctcgag
     1021 ccggccgcgc tcgccaatgc ggcgtcgcgc gcatgggcgg tcgggcggcc caacgcggcg
     1081 agcgacctcg ccgacctcgt cgagatgacc ggccgcgacc tcggcgcgga cccgatcccg
     1141 gccgaggaga tgatcctcac cccccttccc agcggggcct tcgcctgatg gtggcacggg
     1201 ggatgagagg ggtcgccaag gatatcggca ccatccattt cgtcggcatc ggcggcatcg
     1261 gcatgtctgg catcgccgag gtgatgcaca tcctcggcta caaggtgcag ggctccgacg
     1321 tcgccgaggg ctatgtcgtg gaagggcttc gcaaggccgg catcccggtc aaggtcggtc
     1381 accatcccga caatctcggc gactcggcgg tggtggtgat ctcgaccgcc atcaagaagg
     1441 acaatcccga gctggtctgc gcctatgagc gccggatccc ggtggtgcgg agggccgaga
     1501 tgctcgccga gctgatgcgg ctgaaatcga ccgtggcggt ggccgggacc cacggcaaga
     1561 cgacgacgac ctcgatgatc gcggcgatgc tcgatgcggg cgggatcgac ccgaccgtga
     1621 tcaacggcgg gatcatcaac gcctatggct ccaacgcgcg gctcggaagc tccgactgga
     1681 tggtggtcga ggcggacgag agcgacggaa gcttcctgag gctcgacggg accctggcgg
     1741 tggtcaccaa catcgatccc gagcatctcg accattacgg ctcgttcgac aaagtcaagg
     1801 acgccttcgt cgagttcgtc gagaacgttc ccttctacgg cgcggccctg ctctgcgtcg
     1861 accatccgga ggtgcagacg atcatccccc ggcttcgcga ccggcgggtg gtgacctacg
     1921 gcttcgcggc cctggccgac gtccgcggcg agaacgtccg gccggtgccg ggcggcaatc
     1981 tgttcgacgt ctcgctccgc gaccgctccg gcgacgtcgc caccatcgcc gacatcgagc
     2041 ttccaatgcc ggggcgccac aacgtccaga acgcgctggc cgcgatcggg atcgccgcgg
     2101 agctcggcat cccccactcc acgatcgcca ggggcttcga gaagttcggc ggggtcaaga
     2161 ggcgcttcac caaggtcggc gagatcgagg gcgccaccat catcgacgat tacggccacc
     2221 atcccaccga gatccgggcc gttctgtcgg cggcgcgcga gggcgcccag gggcgggtga
     2281 tcgcggtcgt ccagccgcat cgctataccc gcctgcgcga cctgatggag gagttccagg
     2341 gcgccttcaa cgacgccgac gtggtctatg tgactcccgt ctacccggcc ggcgaggagc
     2401 cgatcgaagg agtcgattcc gaggcgctgg ccgagggcct caagcagcgt ggccatcgcg
     2461 cggtcaaggc ggtcgccgac ctcgacgagc tttgcctggg ccttcgcgac ctcgccgcga
     2521 gcggcgacat ggtcatctgc ctcggggccg gcgacatcac caaatgggcc gcggccctcg
     2581 ccgacggaat cgcctgcgcg aggcgggaca aatgagcggc ctccggcacg tcgagacctg
     2641 gggtattcct tccttggagg gtgtcgcgga ggcgaacgga tccctcgcgg acttcatctg
     2701 gttccgcacc ggcggcgctg ccgaatggct ggtacgcccc aaggacgtcg aggatcttgc
     2761 ccgcttcctt cgcgagctgg acccgtcgat gccggtcctg cccgtcggag tcggctccaa
     2821 cctgatcgtc cgcgacggcg gggtgcccgg cgtggtggtc cggctgccca aggcgatgag
     2881 caaggttaat atcgagcccg gcaaccgggt gcgtgcgggc ggcggggcga tgggcatcac
     2941 ggtcgcttcc aaggcgcgcg acgccgatat cgccggcctg gaattcctcc gaggcatacc
     3001 gggcacggcg ggcggcgcgg ttcggatgaa cgccggcgcc tacggacgcg aatccgcgga
     3061 tatcctcgtc gaggcgaccc tggtgcttcg cgacgggcga atcgagacat ggccggccga
     3121 gcggctcggc tacacctatc gccacagcga ggtgcctgcg ggcgcagtgg tcgtcgaagc
     3181 cgtgttccag ggcgtgccgg gcgagcgctc cgcgatcggc gcggagatgg accggatcgc
     3241 cgccgagcgc gaggcgagcc agcctttgcg gagccggacg gggggctcga ccttcaagaa
     3301 tccacccggg accagggcct ggaagctgat cgacgaggcg ggatgccgcg gcctcacgat
     3361 cggcgacgcc caagtctctg aaaagcattg caacttcctc ctgaatctcg gcaaggcgag
     3421 aagctcggac atcgaggcgc tgggcgagga ggtgcggcgg cgggtgctcg agcattcggg
     3481 cgtgttgctt gagtgggaaa tacagcgggt gggagtggcg ggatgatcat tcgtcatccc
     3541 ggcgaaagcc gggaccccag atcgagaagt ctcggcgggt ctccccgagg ccccggcttt
     3601 cgccggggtg acgaatcgtg acctctcccc tccacgtcgc cgtcctcatg ggcggctggt
     3661 cggccgagcg cgaagtctcg ctgatgagcg gcagggacat cgcgagcgcg ctcgagagcc
     3721 tcggccaccg cgtcacccgc atcgacatgg atcgcaacgt ggcccagcgc ctatccgaaa
     3781 tcgctcccga cgtggtcttc aacgccctcc acggtacgcc cggggaagac ggggccgttc
     3841 aggggatgat ggacctgatg ggcctcaaat acacccatag cgggctcgaa acgtcggtca
     3901 tcgccatcga caaggagctg accaagatgg ttctggtgcc ccacggcatt cggatgccgg
     3961 agggacagat cgtcgacagc gaaagccttt atgaaggcga cccgatgtcg cgcccttacg
     4021 tgctcaaacc ggtcaatgaa gggtcttcgg tcggagtggc catcgtcacc gaccagggca
     4081 attgcggcaa gccgatcg
//
LOCUS       DASWNU010000257         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_294755, whole genome shotgun sequence.
ACCESSION   DASWNU010000257 DASWNU010000000
VERSION     DASWNU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294755"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGB57_05075"
     CDS             <1..546
                     /locus_tag="VGB57_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0130757.1"
                     /translation="FDIRKDSLYCDSPSSPKRRACRTVLDTVFHALVRWMAPVLCFTT
                     EEVWQTRYPEDGGSVHLLEWPEIDSGWRDEALGAKWALIRSTRERVTESIEPLRREKV
                     IGSSLEAKVLYPAVQAGLLDSELDQLAEIYIVSEVAPGETDSLEVTRSDYLKCGRCWR
                     LLPEVEEDGGLCARCEGVLNG"
     gene            539..1066
                     /gene="lspA"
                     /locus_tag="VGB57_05080"
     CDS             539..1066
                     /gene="lspA"
                     /locus_tag="VGB57_05080"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY0130758.1"
                     /translation="MADLSSDAERMRKNRRIGLSVAALVFLVDQAVKWVVTYPLQLPH
                     RLTIDVLPFFTLRWLKNTGVSMGLLNAGSEVGRWLLVALTCAIAAFVAVWMWREKRRD
                     DTVALSLVLGGALGNILDRVRLGYVVDYADLHFGDIHPFLVFNVGDAAITIGVLLLLV
                     RALLTRDRKVPEEDK"
     gene            1069..1485
                     /locus_tag="VGB57_05085"
     CDS             1069..1485
                     /locus_tag="VGB57_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEY0130759.1"
                     /translation="MRIVKLGLAACTATLVLAGCGSGGIAGRERPDEFAVTRNQPLVI
                     PPDYALTPPRTGEPTPVGADARTQALEALFGGPAPRSQSERSMLNSAGSDRVALGARS
                     VAGDPGTTVVDKGATTQAIVAAPAGAGRDSTVTTPQ"
     gene            1664..2023
                     /locus_tag="VGB57_05090"
     CDS             1664..2023
                     /locus_tag="VGB57_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130760.1"
                     /translation="MTQQQPPFYVPGLADAGAQIAQLRQVLALVEEMAGRASLSPKDE
                     VLDEAATVSAAYARALPIDQKRFDALAAETARWAAAGVGALLKLEEAGMPVRAAADRL
                     AEELEKGLAALGRRLPG"
BASE COUNT          314 a          756 c          732 g          347 t
ORIGIN      
        1 ttcgatatcc gcaaggacag cctctattgc gactcgccgt cgtcgccgaa gcggcgcgcc
       61 tgtcgcaccg tgctcgacac cgtcttccac gcgcttgtgc gctggatggc gccggtgctg
      121 tgcttcacca ccgaggaagt gtggcagaca cgctatccgg aggatggcgg ctccgtccac
      181 ctgctcgagt ggccggagat cgactccggc tggcgcgacg aagcgctcgg ggcgaaatgg
      241 gccctgatcc gctcgacccg cgagcgggtc accgagagca tcgagccgct gcgccgcgag
      301 aaggtgatcg gctccagcct ggaggcgaag gtgctctacc ccgccgtcca ggccgggctg
      361 ctcgactccg agctcgacca gctcgccgaa atctacatcg tctccgaggt cgcgccgggc
      421 gagaccgaca gcctcgaggt gaccagatcc gactatctga aatgcggccg ctgctggcgg
      481 ctgcttccgg aggtggagga agacgggggc ctgtgcgccc gctgcgaggg ggtattgaat
      541 ggctgatctg tcaagcgacg ccgagcggat gaggaagaac cggagaatcg gcctgtcggt
      601 cgcggccctg gtcttcctcg tcgaccaggc ggtgaaatgg gtggtgacct atccgctgca
      661 gctgccgcat cggctgacca tcgacgtcct gcccttcttc accctgcgct ggctcaagaa
      721 taccggagtc tcgatgggcc tgctcaacgc gggcagcgag gtcgggcgct ggctgctcgt
      781 cgccctcacc tgcgcgatcg ccgccttcgt cgccgtctgg atgtggcgcg agaagcgccg
      841 cgacgacacc gtggcgctct ccctggtgct gggcggggcc ctgggcaaca tcctcgaccg
      901 ggtgcggctc ggctatgtcg tcgactatgc cgacctgcat ttcggcgaca ttcacccctt
      961 tttggtcttt aacgtcggcg acgctgccat taccatcggc gtcctgctat tgctggtccg
     1021 ggcgctgctg acgcgcgacc ggaaggttcc cgaggaggat aagtgactat gcgtatcgtg
     1081 aagctgggcc tcgccgcctg cacggccacc ctggtgctgg ccggctgcgg ttccggcggc
     1141 atcgccggcc gggagcggcc cgacgagttc gcggtcaccc gcaaccagcc cctcgtcatc
     1201 ccgcccgatt acgctttgac cccgccgcgc accggggagc cgaccccggt cggagccgac
     1261 gcgcgcaccc aggcgctcga ggctttgttc ggaggcccgg cgccgcgcag ccagagcgag
     1321 cgctcgatgc tcaactcggc cggaagcgac cgcgtcgccc tcggcgcccg ctcggtcgcc
     1381 ggcgatcccg ggacgaccgt cgtggacaag ggcgcgacca cccaggcgat cgtcgccgcc
     1441 ccggccggcg ccggccgcga ttcgacggtg acgaccccgc agtaaaggac ttccctctcc
     1501 ctgcagggga gagggacagg cgagcgaagc tcgccagggt gagggcacgg cgccgtcgat
     1561 tcaggccctc accctcccac cgcttcgcgg cgggcccctc cctctcccct tcagggagag
     1621 gggttaaacc cttccttagc ccccttccct tatcctgccg cgcatgacgc agcagcagcc
     1681 gcctttctac gtccccggcc tggccgatgc cggcgcccag atcgcgcagc tcaggcaggt
     1741 gctcgctctc gtcgaggaga tggccggccg ggcctcgctt tcgcccaagg acgaggttct
     1801 cgacgaagcc gccacggtga gcgccgctta cgcccgcgcc ctgcccatcg accagaagag
     1861 gttcgacgct ctcgccgccg agaccgcgcg ctgggcggcg gccggggtcg gagcgctgct
     1921 caagctcgag gaggccggaa tgcccgtgcg cgccgccgcc gaccgcctcg ccgaggagct
     1981 tgagaagggg cttgcggcgc tgggccggag gcttccgggc tgatttttgc ctccctatcg
     2041 ccggaggcga tggggagggg gaccatgcga agcatagtgg aggggtagcc ggcgctgcag
     2101 ccggagggtt cgctccagaa gctttaaggc cgagccattt acccctcca
//
LOCUS       DASWNU010000258         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_243287, whole genome shotgun sequence.
ACCESSION   DASWNU010000258 DASWNU010000000
VERSION     DASWNU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243287"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..655)
                     /locus_tag="VGB57_05095"
     CDS             complement(107..655)
                     /locus_tag="VGB57_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0130761.1"
                     /translation="MTDVARRNGRTAGLAALLVLAMVGLAFASVPLYRLFCQVTGIDG
                     TTQRAAEAPGPVAGKEVAVRFDANIAPSLPWRFGPEKAREIVTIGEREMAFYTAKNLS
                     ARPVKGTATFNVTPVQAGKYFSKIQCFCFNEQVLKPGEEVRMPVVFFVDPRILQDPDA
                     KAISEITLSYTFYPVDSGGEGS"
     gene            complement(652..783)
                     /locus_tag="VGB57_05100"
     CDS             complement(652..783)
                     /locus_tag="VGB57_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130762.1"
                     /translation="MTDPRDEEIRRRQKARAIIMALLLGAFVILVYAITIAKMGMQP"
     gene            complement(780..>1691)
                     /locus_tag="VGB57_05105"
     CDS             complement(780..>1691)
                     /locus_tag="VGB57_05105"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0130763.1"
                     /translation="YMTTVTATHDTALPADWRDLLALTKPRVMSLVVFTALCGMLAAP
                     SAIHPVLGFTAILCIALGAGAAAALNMWYEADLDALMKRTAGRPLPAGRLDRQTALHF
                     GVGLAVFSVLLMGLAANWLAAIILAVSILFYVLVYTVWLKRRTAQNIVIGGAAGAFPP
                     MIGWAAATGDVTLLPVLLFTLIFLWTPPHFWALSLFVRSDYAAAGVPMLPVVSGAKAT
                     RQQIALYTLPMAAAAVAPWPLGLAGAFYGIVAAALSLAFLVLALRVLANPATEPAAMK
                     PEKQLFAYSIMYLFAVFGALVVDRVLS"
BASE COUNT          299 a          540 c          604 g          248 t
ORIGIN      
        1 accagcggcc agatgctggg gttcaccaga tggtaggaat ggttcttcgc tccggccatt
       61 tgtcgtcctc tgtcttgccg tcgtcccagc ggtcgtgccg gggtctctag cttccctctc
      121 cgcccgaatc caccgggtaa aaggtgtagc tcaaggtgat ttcgctgatc gctttcgcgt
      181 ccggatcctg gaggatgcgc gggtcgacga agaagacgac cggcatccgc acctcctcgc
      241 ccggcttcag cacctgctcg ttgaagcaga agcactggat cttgctgaaa tacttgccgg
      301 cctggacggg cgtgacgttg aacgtcgcgg ttcccttgac cggccgcgcc gagaggttct
      361 tcgccgtgta gaaggccatt tcgcgctcgc cgatggtgac gatctcgcgg gccttctccg
      421 gcccgaagcg ccagggcagg gacggggcga tattggcgtc gaagcggacc gccacctcct
      481 tgccggcgac cggccccggc gcctccgcgg cgcgctgcgt ggtgccgtcg atcccggtca
      541 cctggcagaa caatcggtag agcggcacgc tggcgaaggc cagtccgacc atcgcgagca
      601 cgagcagggc ggcgaggccg gcggtgcggc cgttgcggcg ggcgacgtcg gtcacggctg
      661 catgcccatc ttggcgatgg tgatcgcgta gaccagaatg acgaaggcgc cgagcagaag
      721 cgccatgatg atcgctcgcg ccttctgccg ccgcctgatc tcctcgtcgc ggggatcggt
      781 cacgaaagca cccggtcgac cacgagcgct ccgaacaccg cgaacagata catgatcgaa
      841 taagcgaaga gctgcttctc aggcttcatc gccgccggtt cggtcgccgg gttggcgagg
      901 acccgaaggg cgaggacgag gaaggcgagg ctgagcgccg ccgcgacgat cccgtaaaag
      961 gcgccggcga ggcccagcgg ccagggcgcc acggccgccg ccgccatcgg cagggtgtag
     1021 agcgcgatct gctgccgggt ggccttcgcg ccggagacca ccggaagcat cggaacgccc
     1081 gcggcggcgt aatcggagcg gacgaacagc gacagcgccc agaaatgggg cggcgtccaa
     1141 aggaagatca gggtgaagag gaggaccgga agcaaggtga cgtcgccggt cgccgccgcc
     1201 cagccgatca tcggcgggaa ggccccggcg gcgccgccga tgacgatgtt ctgcgccgtc
     1261 cgccgcttca gccacacggt gtagacgagg acgtagaaga ggatcgagac cgcgaggatg
     1321 atcgccgcca gccaattggc cgcgagcccc atcagaagca ccgagaagac ggcgaggccg
     1381 actccgaaat ggagggcggt ctgccgatcg aggcgaccgg ccggaagcgg ccgtcccgcg
     1441 gtccgtttca tcagggcgtc gagatccgct tcgtaccaca tgttgagcgc cgccgcggcg
     1501 cccgccccga gcgcgatgca caggatggcg gtgaagccga gcaccgggtg gatcgccgag
     1561 ggcgcggcca gcattccgca cagagcggtg aagacgacga ggctcatcac ccgcggtttg
     1621 gtcagggcga gcaggtcccg ccagtcggcg gggagggcgg tgtcgtgggt ggcggtcact
     1681 gtggtcatat a
//
LOCUS       DASWNU010000259         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_502791, whole genome shotgun sequence.
ACCESSION   DASWNU010000259 DASWNU010000000
VERSION     DASWNU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502791"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VGB57_05110"
     CDS             complement(<1..1338)
                     /locus_tag="VGB57_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY0130764.1"
                     /translation="MAERDYIRATRERVVVFDGGMGATLEQFELTQADYGGLEGKCHE
                     ALVLNRPDVIEGVHASMLEAGAEVVETDTFQGSRLKLEEWGLGEHTLEINTKAAEIAR
                     RAAGDERFVAGSIGPTGFLPASTDPTLGDISFGRLVEVFAEQSRGLVEGGADLIMIET
                     AQDILEVKAAIFGAREAFAATGRALPIQCSVSLLPNGGKMLLGTDIQSVLTTLTALEV
                     DVIGLNCSTGPEDMRDAIRFLGESCPLPVHCIPNAGLPLQGPDGETIFPEKPEPLAAT
                     LGEYVERYGVGIVGGCCGTTPEHIAAIRARVDGHTPGERPGPGPIEVSSMMTSTPLVQ
                     EPRPTLVGERVNSQGSKKAKELLLADDYDGLVQVAEDQVEGGAHVLDTCVALTERQDE
                     DEQMSAVVKRISLTQPAPIQVDSTEPEVIKAALETIPGRAIVNSVNLEAGRDKL"
BASE COUNT          239 a          584 c          581 g          294 t
ORIGIN      
        1 gagcttgtcg cggccggcct cgaggttgac cgagttgacg atcgcccggc cgggaatcgt
       61 ctccagcgca gccttgatca cctcgggctc ggtcgagtcg acctggatcg gggcgggctg
      121 ggtgagcgag atccgcttca cgaccgcgct catctgctcg tcctcgtcct ggcgctcggt
      181 cagcgccacg caggtgtcga gcacgtgggc gccgccttcg acctggtctt cggcgacctg
      241 gacgaggccg tcgtagtcgt cggccagcag cagttccttg gcttttttcg agccctggga
      301 gttgacccgc tcgccgacca gggtcggccg cggctcctgc accagcgggg tcgaggtcat
      361 catcgaggag acctcaatcg ggccggggcc ggggcgctcg ccgggggtgt ggccgtcgac
      421 gcgggcgcgg atcgcggcga tgtgctcggg cgtggtcccg cagcagccgc cgacgatgcc
      481 gacgccgtag cgctcgacgt actcgcccag cgtcgccgcc aggggctcgg gcttttcggg
      541 gaagatcgtc tcgccgtcgg gcccttgcag cggcagcccg gcgttgggga tgcagtggac
      601 cggcagcggg cagctctcgc cgaggaagcg gatcgcgtcg cgcatgtcct cggggccggt
      661 cgagcagttg aggccgatca cgtcgacctc gagcgcggtc agggtcgtca gcaccgactg
      721 gatgtcggtg ccgaggagca tcttgccgcc gttcggcagc agcgagacgc tgcactggat
      781 cggcagcgcc cggccggtcg cggcgaacgc ctcgcgggcg ccgaagatcg ccgccttgac
      841 ctcgaggatg tcctgcgcgg tctcgatcat gatcaggtcg gcgccgcctt cgaccaagcc
      901 gcgggactgt tcggcgaaga cctcgaccag ccggccgaag gagatgtccc cgagggtggg
      961 gtcggtggag gcggggagga agccggtagg gccgatcgag ccggcgacga agcgctcgtc
     1021 accggcggcg cgccgggcga tctccgccgc cttggtgttg atctcgagcg tgtgctcgcc
     1081 cagtccccac tcctcgagct tcagccgcga cccctggaag gtgtcggtct cgaccacctc
     1141 ggcgcccgct tcgagcatcg aggcatgaac cccctcgatc acgtccggcc ggttgagaac
     1201 caaagcctca tggcatttgc cctccaaccc accgtaatcc gcctgagtca gctcaaactg
     1261 ctcgagagtc gcacccatac cgccgtcgaa gacgaccacc cgctcacggg ttgcccttat
     1321 gtagtcacgc tcggccatga acctaagaag gctaccgagg ggggcgggta gtccctcccc
     1381 agacactcaa cctcgagcca tagcgagagg tgtctgggag gggtcacccg ctcgcccctt
     1441 ccgccaccgc ttgagcggta gtggcaaccc tcacgaagtg gcttcgcaga gccattcgcg
     1501 cccccgtttg gatatagtcg cccctgcgcc cgctctgccc cgagccgggc gtcttttatg
     1561 tctcctccca agtacgttga tctactggcg ctgatcgccg cgctcgccgt cttcctgctc
     1621 ggcgggtttc ccctgctcgg cttcgctgcg gcagcggctg tatggctcgc gcagaggggg
     1681 attcaggtcc ttgcccag
//
LOCUS       DASWNU010000260         6049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_10574, whole genome shotgun sequence.
ACCESSION   DASWNU010000260 DASWNU010000000
VERSION     DASWNU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10574"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VGB57_05115"
     CDS             <1..1076
                     /locus_tag="VGB57_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY0130765.1"
                     /translation="PLEIHEVDLEGPKAGEVLVEIMATGICHTDAYTLEGKDSEGIFP
                     SILGHEGAGIVREVGEGVTSVRPGDHVIPLYTPECRQCKSCLSRKTNLCTAIRGTQGK
                     GVMPDGTSRFRLGSETIHHYMGCSTFSNFTVMPEIAVAKVREDAPFDKICYIGCGVTT
                     GVGAVIWTAGVEPGANVVVFGLGGIGLNVIQGARLVGADRIVGIDVNPAKRAMAEAFG
                     MTHFINPNEVGFDKVVPAVLDVTDGGADYSFDCTGNTDVMRQALECCHRGWGKSVIIG
                     VAEAGREIATRPFQLVTGRVWTGSAFGGARGRTDVPRIVDWYMDGKIEIDPLITHTLP
                     LDDINKAFDLMHSGESIRSVVVY"
     gene            1190..2038
                     /gene="fghA"
                     /locus_tag="VGB57_05120"
     CDS             1190..2038
                     /gene="fghA"
                     /locus_tag="VGB57_05120"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY0130766.1"
                     /translation="MTLDTLSVTRAHGGTQHVVKHRSRATGTDMIFSVFVPPAADEGA
                     LCPVIWYLSGLTCTHANVTEKGEYRRACAELGLVFVAPDTSPRGEGVPDDPAGAYDFG
                     LGAGFYVDATQAPFDRHYRMESYVTDELPQVVASAFPVALDRQAITGHSMGGHGALTL
                     ALRNPGRYRSVSAFAPIVAPGQVPWGEKALGAYLGPDRAAWRAHDAVALIEDGARVPE
                     ILVDQGAADSFLEGQLRPELLEAACRDAGIPLTLRMQPGYDHSYYFISTFMEDHLRWH
                     FERLSA"
     gene            2035..2820
                     /gene="cysQ"
                     /locus_tag="VGB57_05125"
     CDS             2035..2820
                     /gene="cysQ"
                     /locus_tag="VGB57_05125"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0130767.1"
                     /translation="MRGSRETPVIPILIEASLAAGAEIERIYIEGCEVVEKEDGSPVT
                     IADRRAEAIILDRLAAAFPGIPVLAEEEAAAGRIPELGTRFFCVDPLDGTRGFIQRNG
                     EFTVNIGLIEDGVPIAGVIYAPVPNLLYHGARGQGAFRVRDGGGAEPIRPRPRPASGL
                     TAVGSRSHAAHGTAEKGAALGISEFVPSSSSLKFCLVAEGTADLYPRHGTTAEWDTAA
                     GQAILEAAGGRVMALDEEGREIGPLTYGKIEQRFLNPHFIAWG"
     gene            complement(2951..5008)
                     /locus_tag="VGB57_05130"
     CDS             complement(2951..5008)
                     /locus_tag="VGB57_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130768.1"
                     /translation="MANKAVWAGLAAAAALLSATGAMAAQAPGADGRTVYEAKYFAQF
                     APSNALQMVQRVPGFTLELGNQEVRGFGQAAGNVVVNGQRPSSKSDTLKTVLARIPAS
                     RVARVEVGPGDLFGSEFAGKPQVVNVVTTGAGGLAGTFNGQLRRTYTGRVEPDATVSA
                     LLRRGKSSFNASAGYQALPTTEAGFDRVTALPSGRLIEYREKVNRIADRTAFVSGAWE
                     HNGGDNRTAHLNFRVSQNWFDLRQTNDVFPAAGPIRDDRLTQDYSRRNYELGGDVTRP
                     LWGGGLKLIGLATRRERRDVDTSLNRIGSEVIGGFIQDIDNLRDERVLRLVWNRSDLG
                     GWSVETGMEGVLNRLDSDVNFYRLAAGGGQTRIDLPVDQAVVEEYRGEAFVNAGRALS
                     KTLRMDLGLTYEASRLTVSGDADAERVLKFFKPKASLDYRDPSGWHAQLSIARTVAQL
                     NFDDFISTAELTNDRVNGGNADLVPQRAWEILATLEQPILGDGMVKVQAGYNRISLVQ
                     DRIPTPEGFDAPGNLGNGRMLILKSTIDAPLAMLGIKGGRLTVNSSLIDTSVEDPYTL
                     RKRQFSGFNEFAADVRFRQDLGKWAYGATFFYNRPTFFFRQDEIDRPWSSQPYVEAFV
                     EYRPSPKTTITFSLDNATDAPASRTRTFFSPDRRTPFPDEFEQRHRNKHIIPAIGVKH
                     SFG"
     gene            complement(5257..5694)
                     /locus_tag="VGB57_05135"
     CDS             complement(5257..5694)
                     /locus_tag="VGB57_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abscisic acid-deficient protein Aba4 family
                     protein"
                     /protein_id="HEY0130769.1"
                     /translation="MPKLESWFVGAHAVALVGWAALFVLPWIGRRRAVLAARWATVPL
                     CLLYLLFLLTHLKAIPTDAGYTLAAIARAFDRPVLLLAGWIHYLVLDLFVGSWQAEHS
                     EKAGVPHALLLPCLFATLMIGPLGLLLYLGVAAWRRRKGALDA"
     gene            complement(5714..5950)
                     /locus_tag="VGB57_05140"
     CDS             complement(5714..5950)
                     /locus_tag="VGB57_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HEY0130770.1"
                     /translation="MADNPFTKHPNEAGESYPEHLGVAARFGARMIVGGLACFVHAVF
                     PFLCERTGSETVRELNAKLGKRVDKPNWERHPII"
BASE COUNT          992 a         2153 c         1953 g          951 t
ORIGIN      
        1 gcccgctgga gatccacgaa gtggatctgg aagggcccaa ggcgggcgag gtgctcgtcg
       61 agatcatggc gacgggaatc tgccataccg atgcctatac gctcgaaggc aaggacagcg
      121 agggcatctt cccctcgatc ctgggccatg agggcgccgg catcgtccgc gaggtgggcg
      181 agggcgtgac ctcggtcagg cccggcgacc acgtcatccc gctctacacc cccgaatgcc
      241 gccagtgtaa atcctgcctg agccgcaaga ccaacctgtg caccgccatc cgcggcaccc
      301 agggcaaggg cgtcatgccc gacggcacca gccgcttccg cctcgggagc gagacgatcc
      361 accactacat gggctgctcg accttctcca acttcacggt gatgccggag atcgcggtgg
      421 cgaaggtccg cgaggacgct ccgttcgaca agatctgcta catcggctgc ggagtgacca
      481 cgggcgtggg cgcggtgatc tggaccgccg gagtggagcc gggcgccaac gtcgtcgtct
      541 tcggcctcgg cgggatcgga ctcaacgtca tccagggcgc caggctggtc ggcgccgacc
      601 ggatcgtcgg gatcgacgtc aacccggcca agcgcgccat ggccgaggcg ttcgggatga
      661 cccatttcat caacccgaac gaagtcggct tcgacaaggt ggtcccggcc gtcctcgacg
      721 tcaccgacgg cggcgccgac tacagcttcg actgcaccgg caacaccgac gtcatgcgcc
      781 aggccctcga atgctgccat cgcggctggg gcaagagcgt catcatcgga gtcgccgaag
      841 cgggccgcga gatcgccacc cgtccgttcc agctcgtcac cggccgggtc tggaccggct
      901 ccgccttcgg cggcgcgagg ggccgcaccg acgtgccgag gatcgtcgac tggtacatgg
      961 acggcaagat cgagatcgac ccgctcatca cccacaccct gccgctcgac gacatcaaca
     1021 aggccttcga cctgatgcac tcgggcgaga gcattaggag cgtggtggtt tattgacgcc
     1081 ccccgtcatt gacgcccccc gtcatcccgg cgaaagccgg gacctcgggg agacccgccg
     1141 agacttctcg ttccgaggtc ccggctttcg ccgggatgac ggagtaaaaa tgaccctcga
     1201 taccctttca gtcacccgcg cccacggcgg cacccagcat gtggtgaagc acaggagccg
     1261 agccaccggc acggacatga ttttctcggt cttcgttcct cccgcggccg acgaaggcgc
     1321 cctctgcccc gtgatctggt atctctcggg cctcacctgc acccacgcca acgtcaccga
     1381 gaaaggcgaa tatcgccgcg cctgcgccga gctcggcctc gtcttcgtcg ctccggacac
     1441 cagcccccgg ggcgaaggcg tccccgacga tcccgccggc gcctatgatt tcgggctcgg
     1501 cgccggcttc tacgtcgacg cgacgcaggc gcccttcgac aggcactatc ggatggaaag
     1561 ctacgtgacg gacgagcttc cgcaagtcgt tgcctcggcc tttccggtcg ccctcgatcg
     1621 ccaggcgatc accggccatt cgatgggcgg ccacggcgcc ctcaccctcg cgcttcgaaa
     1681 ccccggccgc taccgaagcg tctccgcctt cgccccgatc gtcgcgcccg gccaggtgcc
     1741 atggggcgag aaggcgctcg gcgcttatct cggcccggac cgcgccgcct ggcgcgccca
     1801 cgacgccgtc gccctgatcg aggacggcgc ccgcgtcccg gagatcctgg tcgaccaggg
     1861 cgccgccgac tccttcctcg aaggccagct caggcccgag ctgctcgaag cggcctgccg
     1921 cgacgccggc attccgctga ctctgaggat gcagcccggc tacgaccaca gctattattt
     1981 catctccacc ttcatggagg atcacctgcg ctggcatttc gagcggctct cggcatgagg
     2041 ggcagccgcg aaacgcccgt catccccatc ctgatcgaag cctcgctcgc cgccggcgcc
     2101 gagatcgagc gcatctacat cgagggctgc gaggtcgtgg agaaggagga cggctccccc
     2161 gtcaccatcg ccgaccgccg cgccgaggcg atcatcctcg atcgtctcgc cgccgccttt
     2221 ccgggcatcc cggtcctcgc cgaggaggaa gccgccgccg gccggatccc ggagctcggc
     2281 acccgcttct tctgcgtcga cccgctcgac ggcacccgcg gcttcatcca gcgcaacggc
     2341 gagttcaccg tcaacatcgg cctgatcgag gacggcgtgc cgatcgcggg cgtgatctac
     2401 gcgcccgtcc cgaatctgct ttatcacggc gcccgcggcc aaggcgcctt ccgggtccgc
     2461 gacggcggcg gcgccgagcc gatccgcccc cgcccgcgcc ccgcctcggg cctgaccgcc
     2521 gtcggcagcc gaagccacgc cgcccatggc accgccgaga aaggcgcggc tctggggatc
     2581 tcggaattcg tcccgtccag ctcgtcgctc aaattctgcc tggtcgccga aggcacggcc
     2641 gacctctatc cccgccacgg caccaccgcc gaatgggaca ccgccgcggg gcaggccatc
     2701 cttgaagccg ccggcggccg cgtcatggcg ctggacgagg agggccgtga gatcggcccg
     2761 ctgacctacg gcaagatcga gcagcgcttc ctcaaccccc acttcatcgc ctggggctga
     2821 cgtcggaaga gagagcggca gcgttgtcct ctcccgtcat tgcgaggagc gcagcgacga
     2881 agcaatccag cttcggcgcc tgcgtcacgc gcagcactgg attgcttcgc ttcgctcgca
     2941 atgacgggat tcacccgaag ctgtgcttca ccccgatggc gggaatgatg tgcttgttgc
     3001 ggtgccgctg ctcgaactcg tcggggaagg gggtccgccg gtccggcgag aagaaggtgc
     3061 gggtgcggga cgcgggcgcg tcggtggcat tgtcgaggct gaaggtgatc gtcgtcttcg
     3121 ggctcggccg gtattcgacg aaggcctcga cataaggctg gctgctccac ggccggtcga
     3181 tctcgtcctg gcggaagaag aaggtcggcc tgttatagaa gaaggtcgct ccataggccc
     3241 atttgccgag gtcctggcgg aacctgacgt cggcggcgaa ctcgttgaac cccgaaaatt
     3301 gccgcttgcg cagcgtgtag ggatcctcga ccgacgtatc gatcagcgag ctgttcacgg
     3361 tgaggcgccc gcccttgatc cccagcatgg ccaggggagc gtcgatcgtg ctcttgagga
     3421 tcagcatccg cccgttgccg agattgccgg gcgcgtcgaa gccttccggg gtcgggatcc
     3481 ggtcctggac cagcgagatc cggttgtagc ccgcctggac cttgaccatt ccgtcgccga
     3541 ggatcggctg ctccagagtg gccagtatct cccaggcccg ctgcggcacg agatcggcat
     3601 tgccgccgtt gacccggtcg ttggtgagct cggccgtgct gatgaaatcg tcgaaattga
     3661 gctgggcgac ggttcgggcg atcgacagct gggcgtgcca gccggacggg tcgcgatagt
     3721 cgaggctggc cttgggcttg aagaatttca gcaccctttc ggcgtcggca tcgccgctca
     3781 ccgtcagccg cgacgcctcg taggtgaggc cgaggtccat cctcagggtc ttcgaaagcg
     3841 cccgccccgc gttgacgaac gcctcgccgc gatattcctc gaccaccgcc tggtcgacgg
     3901 gaaggtcgat ccgcgtctgc ccgccgcccg ccgcgagccg gtagaaattg acgtcgctgt
     3961 cgagcctgtt gagcacgccc tccattccgg tctcgacgct ccagccgccg aggtcggagc
     4021 ggttccagac cagcctgagc accctctcgt cgcgaagatt gtcgatgtcc tggatgaacc
     4081 cgccgatcac ctcgctcccg atccggttga gcgacgtgtc gacgtcgcgc cgctcccgcc
     4141 gggtggcgag cccgatcagc ttgaggccgc caccccagag cggccgggtg acgtcgccgc
     4201 cgagctcgta gttccggcgg ctgtaatcct gggtgagccg gtcgtcgcgg atcggccccg
     4261 ccgccggaaa gacatcgttg gtctgcctga ggtcgaacca gttctgcgag acccggaagt
     4321 tcaggtgggc ggtgcggttg tcgccgccat tatgctccca ggcgccggag acgaaggcgg
     4381 ttcggtccgc gatccggttc accttctcgc gatattcgat caggcggccg ctcggcaaag
     4441 cggtgacccg gtcgaagccc gcctcggtgg tcggcagggc ctggtagccg gccgaggcgt
     4501 tgaagctcga tttgccccgc cggagcagcg ccgagacggt ggcgtcgggc tcgacccgcc
     4561 cggtataggt gcgcctcagc tgcccgttga acgttccggc gagcccgccc gccccggtgg
     4621 tgacgacgtt gacgacctgc ggcttgccgg cgaattccga tccgaacagg tcgcccggcc
     4681 cgacctcgac ccgggccacc cggctcgccg ggatccgggc cagcacggtc ttgagcgtgt
     4741 cgctcttcga gctcggccgc tggccgttga cgacgacgtt gccggccgcc tggccgaagc
     4801 cgcgaacctc ctggttgccg agctcgagcg tgaagccggg aacgcgctgg accatctgga
     4861 gcgcgttcga cggcgcgaat tgcgcgaaat atttcgcctc gtagaccgtc cgtccgtccg
     4921 cgcccggcgc ctgggcggcc attgctccgg tcgccgagag cagcgccgcc gccgcggcca
     4981 aacccgccca aaccgccttg ttcgccaccg gccacccctt cgaatcatac gctacgtctg
     5041 cgcaacgatc tgcaagaagc acgccatcgc caggcgccta gccgcccgcg acgaacgacg
     5101 ccggagcttc gacaaacgac atgtctgctg tccggggccg gacaaccttt gtccgcaatc
     5161 ggacgcgctg cgtcatcccg gcgaaagccg agacctcggg gagacccgcc gagacttgtt
     5221 gtcccgaggt cccggctttc gccgggatga cgaggatcag gcgtccagcg cgcccttcct
     5281 ccgccgccac gccgcaacgc cgagatagag aagcagcccc agcggcccga tcatcagcgt
     5341 cgcgaacagg caaggcagga gcagggcatg gggaacgccc gccttttcgg aatgctcggc
     5401 ctgccagctc ccgacgaaga gatcgagcac cagataatgg atccacccgg ccagaagcag
     5461 caccggcctg tcgaacgccc gggcgatggc cgccagggtg tagccggcat cggtcgggat
     5521 cgccttcaaa tgcgtgagca ggaagaggag atagagaagg cacagcggaa ccgtcgccca
     5581 ccgcgccgcc agcaccgccc ggcgccggcc gatccacggc aggacgaaca gagcggccca
     5641 ccccaccagg gcgacggcat gcgcgccgac gaaccagctc tcgagcttcg gcatgagccc
     5701 ctcccccgcc ctgctagatg atcgggtgcc gctcccaatt gggcttgtcg accctcttgc
     5761 ccagcttcgc gttcagctcg cgcaccgtct cgcttccggt ccgctcgcac aggaacggga
     5821 agaccgcatg gacgaagcag gcgagccccc cgacgatcat cctcgctccg aagcgcgccg
     5881 ccactcccaa atgctcggga taggattcgc ccgcttcatt ggggtgcttc gtgaagggat
     5941 tgtcggccat ccccgcccct tatcgcgagc cggtccggtt cgccagcgaa tagaaggagc
     6001 gggaggaaac cgtcgtcccg gcaaaagccg ggacctcggg acgagaagt
//
LOCUS       DASWNU010000261         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_87594, whole genome shotgun sequence.
ACCESSION   DASWNU010000261 DASWNU010000000
VERSION     DASWNU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87594"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VGB57_05145"
     CDS             <1..407
                     /locus_tag="VGB57_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0130771.1"
                     /translation="RQAALTDNSEIQIGALAQARGSSPAAREAGRMLQRDHRASQRAA
                     APLMARYRVRPPVGVLPEARTEMRRLQALRGRAFDRELATFNIAAHQRAIALFQAQTR
                     TGDRDTAAFARQQLPVLRHHLQMARDLLRASR"
     gene            560..1003
                     /gene="rpsF"
                     /locus_tag="VGB57_05150"
     CDS             560..1003
                     /gene="rpsF"
                     /locus_tag="VGB57_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0130772.1"
                     /translation="MPLYEHVFIARQDLAQAQVDALAETATNIITTAGGNVVKTETWG
                     LRSLAYRIAKNRKGHYVMLDFEAPAAAVAELERQTQINEDVIRYMTVRVDSHEQGPSA
                     MMRRGERDRERGDRGDRGDRGGRDRDRGDRPDRGDRAPRPDFADA"
     gene            1021..1245
                     /gene="rpsR"
                     /locus_tag="VGB57_05155"
     CDS             1021..1245
                     /gene="rpsR"
                     /locus_tag="VGB57_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY0130773.1"
                     /translation="MARPFFRRRKSCPFSGKDAPVIDYKDVRLLQGFVSERGKIVPSR
                     ITAVSGKKQRELATAIKRARHLGLLPYVVK"
     gene            1259..1888
                     /gene="rplI"
                     /locus_tag="VGB57_05160"
     CDS             1259..1888
                     /gene="rplI"
                     /locus_tag="VGB57_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0130774.1"
                     /translation="MEVILLERIEKLGAIGDVVTVKNGYARNFLLPNGKALRANESNR
                     KVFESNRERIEATNAERRSAAEVDSKGIDGITVQLIRQASNTGQLYGSVSARDLAELL
                     EAEGHKIARNQIVLDRPIKSIGLQEVRIALHPEVSVTIKVNVARSPEEAELQAQGVDV
                     MADMFEKDTAGFTEDYDPNAEPGEIRGDDAPAADTAPAADAGDGEADED"
     gene            complement(2105..>3513)
                     /locus_tag="VGB57_05165"
     CDS             complement(2105..>3513)
                     /locus_tag="VGB57_05165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0130775.1"
                     /translation="SPPAADACGEVKATEGSKLPSTAAAVRSVKDWVDKINSADDAAY
                     VRFVQERGPVLPGGPEQWLELRDFLRGMKLCGVKSARADDVELWVFDPTMDSYGIWRF
                     KPGATETAKTEFAGGSYTDEVPPGAARTAKLPLPALIEALDERAASREAQDQFSGAIL
                     LAKGGRVLFQKAYGLADRERRKPNRLDTQFRFGSMGKMFTAVAIMQLVEKGKVHLEAP
                     IGRYLADYPNKEIATKVTVANLLSHTGGTGNIFGPEFDRHKASLRSTKDYVDLYGGRA
                     PEFAPGSRQSYSNYGFILLGRIVEQVSGLGYDEYIQRNIFRPAGMASTGNWPESEVLP
                     RRAVGYTGSGTRLKSAEDWLPLNGTAAGGGFSTIGDFNRFVGGLTSRRLLRAETLQKL
                     IDGGVKTANGQFAGFDLGETMPDAGRFIGHGGAAPGMNGSLRHFLKSGITVIVLANRD
                     FPAAESLAAFAAHRLPID"
BASE COUNT          601 a         1241 c         1129 g          542 t
ORIGIN      
        1 tccgccaggc cgcgctcacc gacaatagcg agatccagat cggcgctctc gcccaggcgc
       61 gcggaagctc gccggccgcc cgcgaggcgg gacggatgct gcagagggac catcgcgcct
      121 cgcaaagggc cgcggcaccg ctcatggccc gctaccgggt ccggccgccc gtgggcgtcc
      181 tgcccgaggc gaggaccgag atgcgccggc tccaggccct tcggggccgc gccttcgacc
      241 gggagctcgc cactttcaac atcgccgccc atcagcgcgc catcgccctc ttccaggcgc
      301 agactcggac cggcgaccgc gacacggcgg ccttcgcccg ccagcagctg ccggtgctcc
      361 gccatcacct gcagatggcc cgcgacctgc tccgcgcctc gcgctaagag cttgcgaaag
      421 acagggcttc tgctatggag cccgcgaccg gggcggaagc catgcgcttc ccgccccggt
      481 tcaatttcag acgaaagccg gaggggcgtc cgcattgggc ggcgtcagcg atcggccaaa
      541 gacaaggaaa tgcgaagcca tgccgcttta cgagcatgtg ttcatcgcgc gtcaggacct
      601 ggcccaggcc caggtcgacg cgctggcgga gaccgccacc aacatcatca ccaccgccgg
      661 cggcaacgtc gtcaagaccg agacctgggg ccttcgctcg ctcgcctacc ggatcgccaa
      721 gaaccgcaag ggccattacg tgatgctcga cttcgaggct ccggccgcgg ccgtcgccga
      781 gctcgagcgg cagacccaga tcaacgagga cgtgatccgc tacatgaccg tgcgcgtcga
      841 ctcgcacgag cagggcccgt cggccatgat gcgccgcggc gagcgcgacc gcgagcgcgg
      901 cgatcgtggc gaccgcggcg atcgcggcgg ccgcgaccgc gaccgcggcg accgtccgga
      961 tcgcggcgac cgcgcccccc gccccgactt cgccgacgcg taacgagaaa ggacagcaac
     1021 atggcacgcc catttttccg ccgccgcaag agctgcccct tctcgggcaa ggacgctccg
     1081 gtcatcgact ataaggacgt ccgcctcctc cagggcttcg tctccgagcg gggcaagatc
     1141 gtcccgagcc gcatcaccgc cgtctccggc aagaagcagc gcgagctggc caccgcgatc
     1201 aagcgcgccc gccacctcgg ccttctcccc tacgtcgtga aataaggagc gcgaggacat
     1261 ggaagtcatt ctcctcgaga ggatcgagaa gctcggcgcg atcggcgacg tggtgacggt
     1321 gaagaacggc tatgcccgca acttcctcct tcccaacggc aaggcgctcc gggccaacga
     1381 gtcgaaccgc aaggtgttcg agagcaaccg cgagcggatc gaggcgacga acgccgagcg
     1441 ccgctccgcc gccgaggtcg attcgaaggg catcgacggc atcaccgtcc agctcatccg
     1501 tcaggcgtcc aacaccggcc agctctacgg ctcggtgtcg gctcgcgatc tcgccgagct
     1561 gctcgaagcc gaaggccaca agatcgccag gaaccagatc gtcctcgacc gtccgatcaa
     1621 gtcgatcggc cttcaggagg tgcgcatcgc gctccacccg gaggtgtcgg tgaccatcaa
     1681 ggtcaacgtc gcccgctcgc ctgaagaggc cgagctccag gcgcagggcg tcgacgtgat
     1741 ggccgacatg ttcgagaagg acaccgccgg cttcaccgag gattacgatc ccaacgccga
     1801 gccgggcgag attcgcggcg atgacgcccc ggcggcggac accgctccgg cggccgatgc
     1861 tggcgacggc gaggcggacg aagactgatc ccgtcgtccg gcgaaagccg ggacctcggg
     1921 acgagaagtc tcggcgggtc tccaccggat cccggcttcc gccgggatga cgatcaagac
     1981 gaaaaggccg cctgctcctt cgagccggcg gtcttttctt tggcgcctta tgagaggcag
     2041 cgccggaagc gtccggcaaa tgcgggacaa tgcggcactg gcggcgtctc cgcggttcgc
     2101 gcgctcaatc gatgggcagg cggtgagcgg cgaacgccgc gaggctttcc gccgcgggga
     2161 aatcgcgatt ggcgaggacg atcacggtga tcccgctctt caggaagtgc cgaagcgaac
     2221 cgttcattcc cggcgccgcg ccgccatgcc cgatgaaacg cccggcgtcc ggcatcgtct
     2281 cgcctaaatc gaatccggcg aactgcccgt tggccgtttt cacgccgccg tcgatcagct
     2341 tttggagcgt ttcggcgcgc agcagccggc gggacgtcag tccgccgacg aaccggttga
     2401 agtcgccgat cgtcgaaaag ccgccgccgg ccgccgtgcc gttgagggga agccaatcct
     2461 cggcgctctt cagccgggtc cccgatcccg tgtagccgac ggctcggcgc ggcaggactt
     2521 cgctttccgg ccagtttccc gtcgaagcca ttccggcggg cctgaagatg ttccgctgga
     2581 tatactcgtc atatcccagg cccgatacct gctcgacgat ccgcccgagg aggatgaagc
     2641 cgtaattgct gtacgattgc cggcttccag gcgcgaactc gggagctcgg ccgccgtaga
     2701 ggtcgacata gtccttcgtg cttcgcagcg acgccttgtg ccggtcgaac tcgggcccga
     2761 aaatgttgcc cgtgcctccc gtatggctga gcaggttggc gaccgtcacc ttggtcgcga
     2821 tttccttgtt cgggtaatcg gcgagatagc gtccgatcgg cgcctcgagg tggactttgc
     2881 ccttctcgac cagctgcatg atcgcgaccg cggtgaacat cttccccatc gatccgaacc
     2941 ggaactgcgt atcgagccgg ttcggcttgc ggcgctcgcg atccgcgagg ccataggcct
     3001 tctggaacag cactcgaccg cctttcgcca gcaggatcgc gcccgaaaat tgatcctgcg
     3061 cctcccggct ggccgcgcgc tcgtcgaggg cctcgatcaa tgcgggaagc gggagcttcg
     3121 cggttcgagc ggcgcccggc ggaacctcgt cggtgtagga tccgccggcg aattcggtct
     3181 tggcggtctc ggtcgcgccc ggtttgaacc gccagatgcc gtaggagtcc atggtgggat
     3241 cgaataccca cagctccacg tcgtcggccc tggcggattt gactccgcac aatttcatgc
     3301 cgcgtagaaa gtcgcgcaac tccagccact gctccgggcc gccgggcagc acaggcccgc
     3361 gctcctggac gaagcggaca taggcggcat catccgccga gttgatcttg tcgacccaat
     3421 ccttcaccga tcgcaccgcc gccgccgtcg acggcagctt cgatccctcc gtcgccttca
     3481 cctctccgca agcgtcggcc gccggcggcg atg
//
LOCUS       DASWNU010000262         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_398737, whole genome shotgun sequence.
ACCESSION   DASWNU010000262 DASWNU010000000
VERSION     DASWNU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398737"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2423
                     /locus_tag="VGB57_05170"
     CDS             <1..2423
                     /locus_tag="VGB57_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0130776.1"
                     /translation="GALLASLAGTVCMVVILSVVVAIVCVPALLTLLGPNVDRWRLGS
                     TAPAGGRSRLMGMVGAALKKPAVATVLIGALLLVLAVPAIGLKTGPPSPEQLAQDAPA
                     RLDAELIDDSIGAGFDAPFQIVAVSEEGPITDAKDLAALDTFQRRLAKTPGVQTVIGP
                     GRATKRVEPLKELGNAVLSSEGDIGPVKQLGRLGRSLDVAADGVGQLRAGISEAGDGA
                     GLLALGAGRAGEGATMIANGLSQAVGGSERAVNALDRFAKGSQRLTDGLEEASFGALS
                     IKLAVRDGILTNLKQNPLPRSFDLRKSLNQDSNFKLPELIAPARVTDEQLKAALARLQ
                     AMTVGRSDPEFAAALEAVRRAVAAETGVDPVTGAPFDPVPETPEVEPYAGLPAELIAL
                     HERLQENYTDAKEVNFWLLSTRNQLEEVVEGSEQLTEGLEKLRGGSKQLTAGAERLAK
                     EAGRLGNGLSQLSGGTLALVKGLDDLGEGADALQAGLLSGAEESAPLETGLEEASVQV
                     IDGRERIRNQAGRVARTTPGLFNSGYFVLSALDGTPPETRKQISETIDLERGGQAATM
                     LVISDYSFNTEGSIALNKELNERAAKLAEDAGLVTGVAGGAAQLNDYSRVTRDRIPWV
                     VIAITIATFFVLMIVLRAVLLAAIAVLLNLLTVAVAFGVLTLLFNVPDDWPLGGHNYV
                     DAVGATMIFGLVFGLSIDYAVFLLSRMREHYDAHGDNAAAVDFGLSRTAGVITGAAAI
                     MMVVFIAFAGAPIATVSQLGIGLTIAVILDATVVRIVLLPALMLLLGDRVWWLPKPLE
                     RVIPKMTV"
     gene            2643..>2979
                     /locus_tag="VGB57_05175"
     CDS             2643..>2979
                     /locus_tag="VGB57_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0130777.1"
                     /translation="MIVLAVIFLTGNGGHKYTLIFQNAGQLVPDNQVLIGGSPAGTVT
                     SIGLTDDNLAEIEVEIDQELHEGTTATIRATSLSGVANHYVAISPGPNSNEPLDDGAE
                     LGLASTTTPV"
BASE COUNT          485 a         1060 c         1018 g          416 t
ORIGIN      
        1 cgggggcgct gctggcctcg ctggcgggga ccgtctgcat ggtggtgatc ctcagcgtcg
       61 tcgtcgcgat cgtctgcgtg ccggccctcc tcaccctgct ggggccgaac gtcgaccgct
      121 ggcgcctcgg ctccaccgcc ccggccggcg gacgctcgcg gttgatggga atggtcgggg
      181 cggcgctgaa gaagccggcg gtggcgaccg tcctgatcgg ggcgttgctg ctggtgctgg
      241 cggtgccggc gatcgggttg aagaccggcc cgccgagccc cgagcagctg gcccaggacg
      301 cccccgcccg cctcgacgcc gaactgatcg acgactcgat cggggccggc ttcgacgccc
      361 cgttccagat cgtcgccgtc tccgaggaag ggccgatcac cgacgccaag gacctcgccg
      421 cgctcgacac cttccagcgg cggctggcga agaccccggg cgtgcagacg gtgatcgggc
      481 cgggccgggc gacgaaacgg gtcgagccgc tgaaagagct cggcaacgcc gtcctctcct
      541 ccgaaggcga catcggcccg gtcaaacagc tgggccggct cggccgcagc ctcgatgtcg
      601 ccgccgacgg cgtcggccag ctgcgggcgg gaatctcgga agccggcgac ggggcggggc
      661 tgctcgcgct cggcgccggc cgagcgggcg agggggcgac gatgatcgcc aacgggctca
      721 gccaggcggt cggcggaagc gagcgggcgg tcaacgcgct cgaccgcttc gcgaaaggct
      781 cccagcggct gaccgacggc ctcgaagaag cctccttcgg cgccctcagc atcaagctcg
      841 cggtgcgcga cgggattctc accaacctga agcagaaccc gctgccgcga tcgttcgacc
      901 tgcggaaatc gctgaaccag gactccaact tcaaactgcc ggaactgatc gcgccggccc
      961 gcgtcaccga cgaacagctg aaagcggcgc tggcgcggct gcaggcgatg acggtcggca
     1021 ggtcggaccc cgaattcgcg gcggcgctgg aggcggtgcg gcgggcggtc gcggccgaga
     1081 ccggcgtcga cccggtcacc ggcgccccct tcgacccggt gccggagacg ccggaggtcg
     1141 agccctacgc cgggctcccg gccgagctga tcgccctcca cgaacgcctg caggagaact
     1201 acaccgacgc caaagaagtc aacttctggc tgctctcgac ccgcaaccag ctggaagaag
     1261 tggtggaagg ctccgaacag ctcaccgagg ggctggaaaa gctgcggggg ggcagcaaac
     1321 agctgaccgc cggcgccgag cgcctcgcca aagaagcggg caggctcggc aacggcctct
     1381 cccagttgag cggcggcacc ctggcactgg tcaaaggcct cgacgacctc ggcgagggag
     1441 cggacgcgct gcaggcggga ctgctcagcg gcgccgagga aagcgcgccg ctggaaaccg
     1501 ggctggaaga agcgagcgtg caggtgatcg acggccgcga gcggatccgc aaccaggccg
     1561 ggcgcgtcgc ccgcaccacc ccgggcctgt tcaactccgg ctacttcgtc ctctccgccc
     1621 tcgacgggac cccgcccgag acccgcaagc agatcagcga aacgatcgac ctcgagcgcg
     1681 gcggccaggc ggcgacgatg ctggtcatct ccgactacag cttcaacacc gagggctcga
     1741 ttgctctcaa caaggagctg aacgagcgcg ccgccaagct ggcagaggac gcgggcctgg
     1801 tgaccggcgt cgccggcggc gccgcccagc tcaacgacta cagccgcgtc acccgcgacc
     1861 gcatcccctg ggtggtgatc gcgatcacga tcgcaacctt cttcgtcctg atgatcgtcc
     1921 tgcgggcggt gctgttggcg gcgatcgcgg tcctcctcaa cctgctcacg gtggcggtcg
     1981 ccttcggggt gctgacgctc ctcttcaacg tccccgacga ctggccgctg ggcggccaca
     2041 actacgtcga cgcggtcggg gcgacgatga tcttcggcct cgtcttcggc ctctcgatcg
     2101 actacgcggt cttcctgctc agccgcatgc gcgagcacta cgacgcccac ggcgacaacg
     2161 ccgccgccgt cgacttcggg ctctcccgca ccgccggcgt gatcaccggg gccgcggcga
     2221 tcatgatggt tgtcttcatc gctttcgccg gcgccccgat cgccaccgtc agccagctcg
     2281 gcatcggcct caccatcgcc gtcatcctcg acgccaccgt cgttcgcatc gtcttgttgc
     2341 cggcgttgat gctcctgctg ggcgaccggg tttggtggct gccgaagccg ctggagcggg
     2401 tgatcccgaa aatgaccgtc taggcgcgtt gacttccagt taagtccctg cttgcagggg
     2461 acttgccggg gaccccctcg gatacgatcc tccagttgtt ctgccggctc ggagggaccg
     2521 gagaacctga aagcaagcga cgatccgtgg ctgccaaacc cttaggcgag gagagaggcc
     2581 cggcagcgag ccggggaact accccagcca gaattgcgac gatcgcggct ctggcggtcg
     2641 cggtgatcgt gctggcggtg atcttcctca ccggcaacgg cggccacaag tacacgctga
     2701 tcttccagaa cgcgggccag ctggtgcccg acaaccaggt tctgatcggc ggctcgccgg
     2761 ccggaaccgt gaccagcatc gggctcaccg acgacaacct cgccgagatc gaggtcgaga
     2821 tcgaccagga gctgcacgaa gggacgacgg cgacgatccg ggcgacctcg ctctccggcg
     2881 tcgccaacca ctacgtcgcg atcagccccg gcccgaacag caacgagccg ctcgacgacg
     2941 gtgccgagct cggcctcgcc tcgacgacca cgccggtcg
//
LOCUS       DASWNU010000263         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_138793, whole genome shotgun sequence.
ACCESSION   DASWNU010000263 DASWNU010000000
VERSION     DASWNU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138793"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1569
                     /locus_tag="VGB57_05180"
     CDS             <1..>1569
                     /locus_tag="VGB57_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130778.1"
                     /translation="VGTIGSDSLAGLGGDDQLAGGLGADSLDGGEGHDRLSGGGGDDL
                     LTGGGGEDWIEDLDGGNDQMSGGDGADRLVITRSAAGTPGATVFASGGEGADRFDITS
                     HSAASLTLDGGTGDDRFNFFKIEAAARLTLGEGRDTIRLEPEYFGPYTNQTRTEEIVV
                     TDFQTGAGGDFLDLLDGIAYGVMTSGAEISPFLFGGLRFVQDGSSALLQYSYSVTEFQ
                     TLFRFENRNVADFTIDNFGFPIARSILVDQPGAYTHSGDTFGVPGMRIVAEGATVTIG
                     AGSKLQAALGTPSEISAAIAIQASGATVIIEEGATVLARPIWNPETVHTDETAILGSD
                     HSDRIFNYGRTSGMIRLGAGDDYFFERITIPSRDFARLEMGSGDDTIEFDLNGRTNLY
                     SAGLNGGVGNDTLIFSGIVGQASVEGFGTTGIETLRLSGAAGASVSFGGRPPYADTYL
                     SAGLTLSILAPQGSFRPYTVAPSGTLHLQGGNFTTALQAPFASIVGSDSAERVVLSNS
                     FFPGTGITVQAIPSV"
BASE COUNT          258 a          542 c          518 g          251 t
ORIGIN      
        1 gtcggcacga tcgggtccga cagccttgcg ggcctgggcg gcgacgacca gctggcgggc
       61 ggccttggtg ccgattcgct cgacggcggc gaaggccacg accggctttc cggcggcggc
      121 ggtgacgacc tgctgaccgg cggcggcggc gaggactgga tcgaggatct ggacggcggc
      181 aacgaccaga tgagcggcgg cgacggcgcc gaccggctgg tcatcacccg ctcggcggcc
      241 gggactcccg gagccacggt cttcgcaagc ggcggcgaag gcgccgaccg gttcgacatc
      301 accagccaca gcgccgcgag cctcacgctc gacggcggca ccggggacga ccggttcaac
      361 ttcttcaaga tcgaggccgc ggcgcgcctg acgctcggcg agggccgcga caccatccgg
      421 ctggagcctg aatatttcgg cccctatacg aatcagaccc ggacggagga gatcgtcgtc
      481 accgatttcc agaccggtgc gggcggcgac ttcctcgacc tcctcgacgg catcgcctac
      541 ggcgtcatga ccagcggggc ggagatcagc ccgttcctgt tcggcgggct gaggttcgtc
      601 caggacggga gctcggccct gctccaatat tcctattcgg tcaccgagtt ccagacgctg
      661 ttccgattcg agaaccgcaa cgtcgcggac ttcaccatcg acaatttcgg ttttccgatc
      721 gcccggtcga tcctcgtcga ccagcccgga gcctacaccc acagcggcga cactttcgga
      781 gttcccggga tgcgcatcgt cgccgagggc gcgacggtga ccatcggcgc gggctcgaag
      841 ctgcaggcgg cgctgggcac tccgtccgaa atcagcgccg ccatcgccat ccaggcctcc
      901 ggcgcgaccg tcatcatcga ggagggcgcc accgtcctcg ctcggcccat ctggaacccg
      961 gagaccgtgc acacggacga gaccgccatc ctcggctccg atcattcgga ccggatcttc
     1021 aactacggcc gaacgagcgg gatgatcagg ctcggcgcgg gcgacgatta tttcttcgag
     1081 cggatcacca ttccctcaag ggacttcgcc cggctggaaa tgggcagcgg cgacgacacc
     1141 atcgaattcg atctgaacgg ccgtaccaac ctttattcgg ccggcttgaa cggcggggtc
     1201 ggcaacgaca cgctcatctt cagcggcatt gtcggccagg ccagtgtcga gggcttcggc
     1261 acgaccggca tcgagacgct ccggctttcg ggcgccgccg gggcctcggt cagcttcggc
     1321 ggccggccac cctatgcgga cacctatctg tcggccggac tgactttgag cattctcgct
     1381 ccgcaaggct cgttcaggcc ctacaccgtg gcgccgtcgg gaacgcttca cctccagggc
     1441 ggcaatttca cgacggcgct gcaggcgccg ttcgcgagca tcgtgggaag cgactcggcg
     1501 gagcgggtgg tcctgtccaa ctcgttcttc ccggggacgg gaatcaccgt ccaggcgatc
     1561 ccgagcgtc
//
LOCUS       DASWNU010000264         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_347106, whole genome shotgun sequence.
ACCESSION   DASWNU010000264 DASWNU010000000
VERSION     DASWNU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347106"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VGB57_05185"
     CDS             <1..928
                     /locus_tag="VGB57_05185"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0130779.1"
                     /translation="FEAWREAQRNFRAAYAPLLEPGPGRPSHRSFIETIAAQVERAPP
                     MARPKAVPPVAGAAAGHVFLLGYPRSGTTLVENILASAAEVVALEEQDTLADTDGVLV
                     ANDGAMPDLDALDPALPADLRARYWARVRSLAGDVAGRVFVDMNPFNGTRLPIIARLF
                     PDARILIMRRDPRDVVLSCFRINFTPSAGTYAFSDLEETALHYSALMRLIELCRERLP
                     LACHEVRYDRLVGDFEATVRAMAGFAGIAWTEEFHAFDRTAQKRGVQTASATQVRRGL
                     YDGGGRWRRYADQLAPVLPVLAPWVERLGFER"
     gene            complement(1036..>2282)
                     /locus_tag="VGB57_05190"
     CDS             complement(1036..>2282)
                     /locus_tag="VGB57_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0130780.1"
                     /translation="WKGAATWEPFDGLKFRGNYQRAVRAPNIGELFAPLNTGLTTVAT
                     DPCRTTRNTLGEITYAGPLTNANLRAVCLAQGAPADTIGGINNPTAAQANATTAGSLA
                     LRPEKADSFTVGLVFQPDFIPGLSLTVDYYKIEINRAITQPGPGDLINGCFGGNNAQG
                     VTAASATNPICGLFRRNPVTGGLDGDAATTQGLIFPASNAGRILTDGIDFGANYRRDL
                     GFARLNLSFQGNWTNRSEFEPIVPGSVLPPGVPGAGGVLPESGSRECIGLYGPNCGSP
                     GSAGPSSTPGSIQPEWSWNARSTLSFGDIDVSLLWRHISGVEAEPGIVAFAGTLTTGA
                     LAGEEVNFGKIGAYDWFDLALRFGVNENFDLTMTVSNLFDKTPPIVGSTIGTTSFNSG
                     NTYPSTYDALGRRFAVGGRLKF"
BASE COUNT          352 a          760 c          811 g          359 t
ORIGIN      
        1 cttcgaggcc tggcgggagg cgcaacgcaa tttcagggcc gcttatgcgc ccctgctcga
       61 gcccgggccc ggccgcccgt cgcaccgaag cttcatcgag acgatcgccg cgcaggtcga
      121 gcgggcaccg ccgatggcaa ggccgaaggc cgtaccgccg gtcgccggcg ccgccgcggg
      181 ccacgtcttc ctgctcggct atccccgctc ggggacgacc ctcgtcgaga acatcctggc
      241 gagcgcggcc gaagtggtgg cgctcgaaga gcaggacacc ctcgccgaca ccgacggcgt
      301 cctcgtcgcc aatgacggcg cgatgcccga tctcgacgct ctggacccgg cgctgccggc
      361 cgatctgcgc gcccgctact gggccagggt gcgcagcctc gccggcgacg tggcgggtcg
      421 ggtcttcgtc gacatgaacc cgttcaacgg caccaggctg ccgatcatcg cccggctgtt
      481 tccggacgcc cgtatcctga tcatgcggcg cgatccgcgc gacgtcgtgc tgagctgctt
      541 caggatcaac ttcacgccca gcgccgggac ctacgcgttc agcgacctcg aggagacggc
      601 gctccattat tcggcgctga tgcgcctcat cgagctgtgc cgcgagcggc tgccactggc
      661 ctgtcacgaa gtccgctacg accggctggt gggcgatttc gaggcgacgg tccgcgccat
      721 ggcgggtttc gccggaatcg cctggacgga ggaattccat gccttcgacc ggaccgcgca
      781 aaagcgcggg gtgcagacgg cgagcgcgac tcaggtgcgg cgcggactct atgacggcgg
      841 cggccggtgg cgccgctatg cggaccagct cgcgcctgtg ctgccggtcc tcgccccctg
      901 ggtggagcgc ctgggcttcg agcgctaggc tctgcgtcca ggccggccgg aacgggacag
      961 ggccagccaa ccgatcgatg ctcaaaaaaa aggggcggcc cgaaggccgc cccgagagct
     1021 tgcgtatcgt ctgccttaga acttcaggcg accgccgacc gcgaagcggc ggcccagagc
     1081 gtcgtaggtc gacggatagg tgttgccgct gttgaacgag gtcgtgccga tggtcgagcc
     1141 gacgatcggc ggcgtcttgt cgaacaggtt gctgaccgtc atcgtcaggt cgaaattctc
     1201 gttgacgccg aagcgcagcg cgagatcgaa ccagtcgtaa gcgccgatct tgccgaaatt
     1261 gacttcctcg ccggccagag cgcccgtggt gagggtgccc gcgaaggcga cgatgcccgg
     1321 ctcggcttcg actccggaga tgtggcgcca cagcagcgac acgtcgatgt ctccgaagct
     1381 cagggtggag cgcgcgttcc agctccactc gggctggatc gaacccgggg tcgagctcgg
     1441 acccgccgaa cccggcgaac cgcagttcgg accgtagagg ccgatgcact cgcggctacc
     1501 ggactccggc aggacgccgc cggcacccgg cacgcccggc ggaaggacgg agcccggcac
     1561 gatcggctcg aactcggagc ggttcgtcca gttgccctgg aagctcaggt tcaaccgggc
     1621 gaagccgagg tcccgccgat aattggcccc gaagtcgatt ccgtcggtga ggatccgccc
     1681 cgcgttcgaa gccgggaaga tcaggccctg cgtcgtcgcg gcatcgccgt ccagaccgcc
     1741 ggtcaccggg ttgcggcgga agaggccgca aatcgggttg gtggcgctgg ccgccgtgac
     1801 tccctgggcg ttattgccgc cgaagcagcc gttgatgaga tcgcccgggc ctggctgggt
     1861 gatcgcgcgg ttgatctcga tcttgtagta atcgacggtg agcgacaggc ccggaatgaa
     1921 gtcgggctgg aacaccaggc cgacggtgaa gctgtccgcc ttctccggcc tcagcgcgag
     1981 gcttcccgcc gtggtggcgt tggcctgggc ggccgtcggg ttgttgatgc caccgatggt
     2041 atccgccgga gcgccctgag cgaggcagac ggcccggagg ttggcgttgg tgagcggtcc
     2101 ggcataggtg atctcgccga gcgtgttgcg cgtggtgcgg cacggatcgg tggccaccgt
     2161 cgtgaggccg gtattcagcg gcgcgaacag ctcgccgatg ttcggcgcac gaaccgcgcg
     2221 ctggtagttg ccgcggaact tgaggccatc gaacggctcc caggtggcgg cgcccttcca
     2281 gg
//
LOCUS       DASWNU010000265         5155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_425056, whole genome shotgun sequence.
ACCESSION   DASWNU010000265 DASWNU010000000
VERSION     DASWNU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425056"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VGB57_05195"
     CDS             <1..1039
                     /locus_tag="VGB57_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130781.1"
                     /translation="PRRGRMRGSPPLRFLAIVVGGWAALRTALIAPPWSTPPAGAVAA
                     PAQARARPGPGREREAGPPNRERVVALALPVFAAATAPAPRAARGRPRATAMPPFPTP
                     EPARGMLRAAAAAVPPFMAPPGPLAARPDHASTSARRWSLSAWSFVRSGDSRALAAGG
                     LLGGSQAGARFVYRLDRDSARPLAVSFRLSAPLRNARAAEAALGLDWQPDRRLAVHFL
                     AERRQALGRDGRSAFSVTVHGGVSDAPLGRFRIESYAQAGLVGTRSRDPFGDGSLRIS
                     LPVDEKVRLGAGAWAAAQPGLARFDLGPQATLRLPVAGRALTLAADWRLRVAGNARPG
                     SGATLTMSTDY"
     gene            1082..1354
                     /locus_tag="VGB57_05200"
     CDS             1082..1354
                     /locus_tag="VGB57_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0130782.1"
                     /translation="MRGHSPAREVRYSREAARTLLRIDRRTSRRIRAKIDLLAADPGA
                     LGNNVGALKGESGLKRLRIGSWRVIYTDDLTVLLVLKIAPRGSAYD"
     gene            1347..1760
                     /locus_tag="VGB57_05205"
     CDS             1347..1760
                     /locus_tag="VGB57_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130783.1"
                     /translation="MIEDDRMSYEPQTFAAPDGTEMVILKAADYARLKRLCGEEAEDA
                     AEGMKALAEIRDGMGTMPGEVLNSILDEGLTPLAAWRRHRGLSQSALAGRAGLSQVWI
                     SRIERGGGYGSRETRRKLAAALDAPVWALEDERTT"
     gene            1812..2723
                     /locus_tag="VGB57_05210"
     CDS             1812..2723
                     /locus_tag="VGB57_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0130784.1"
                     /translation="MDIYLPIAGLSVNALAIILLGGVVGLLTGMVGVGGGFLTTPILI
                     FYGIPPPVAVASATTQITGTSVSGVLAHRRRKGVDYRMGAVIIAGGLLGALAGGGLFR
                     LLQDAGQIDTVISLLYVVLLGTIGIMMAREAATALDILKPKENSKKPKRHNPLIAALP
                     MRWRFYQSGLYISPLAPLLLGFGGGMLTVLLGVGGGFILVPAMIYLLGMSAQVVVGTS
                     LLQILFVTAATTLIHATTTKSVDIVLAGLLLLGSVIGAQFGARLAQKAKPELLRMFLA
                     IIILAVAIRMAIGLGWRPEEIYTLQVL"
     gene            2720..3481
                     /locus_tag="VGB57_05215"
     CDS             2720..3481
                     /locus_tag="VGB57_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEY0130785.1"
                     /translation="MSRLRALLLLCAAPLALGAAKPRLVPDLSQSSIDIQYSFTGAEL
                     LLFGAILYPGGRTPTEHADIAVVVKGPVQPLVVREKQKVAGIWMNVEAARFRSAPAFY
                     AVASSRPLSKLIDERTAAIYELGLENLSLSPGAGKSAEVQRRFEAGLIDLMRRRRLYS
                     EDPDGVRITDHVLYRGRITFPSLVPVGDYTAETFLIKDGRVIAGAVREIKVGKTGFER
                     EVAAAADRWSFAYGLAAVALSLLMGWGGSLVLRKG"
     gene            3652..>5155
                     /locus_tag="VGB57_05220"
     CDS             3652..>5155
                     /locus_tag="VGB57_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEY0130786.1"
                     /translation="MTDMMNFKTVTLPEETEEFAPTIRPRIPQPVRHAPIGKVVDIRG
                     GGGRIELEVGRVSEIARDPDPSIALSGQVGGHVKLEAGNRWLLANVRSLTLRDGEAGM
                     ISADIDFLGEGEEDEQSGKLINFRRGITGYPPPGARAFPVSGQDMRQMFAADDRAHIE
                     IGTVYPTKDVRGALYVDALLGKHFALLGSTGTGKSTSAALIMHRICDLSPEGHIVMID
                     PHGEYSAAFKSVGELFNVENLAMPYWLMNFEEHCEVFITSAGAERQRDMDILAKCLLL
                     ARSKNRAAEGMTKLTVDSPIPYALSDLAGAINAEMGLLNKATDTAPFMRLKTKIDELK
                     ADPRYHFMFSGMLIADSMTSFIGKIFRLPARGKPISIVDVSGVPSDIVSVVVAVLSRL
                     VFDYAIWSRNEVPRPVLLVCEEAHRYIPSDKTGGGQSVRKILERIAKEGRKYGVSLGL
                     ITQRPSDLAEGVLSQCGTIIAMRLNNERDQHFVKSAMPEGARGFLDVIPAL"
BASE COUNT          782 a         1838 c         1693 g          842 t
ORIGIN      
        1 cccgcgccgc ggccgaatga ggggctcgcc gcccctgcgc ttcctcgcga tcgtcgtggg
       61 agggtgggcc gcgctgagga cggccctcat cgctccgccc tggtcgaccc cgcctgccgg
      121 agcggtcgcg gcgccggcgc aggcccgcgc ccgccctggg cccggccggg aacgggaggc
      181 cgggcctccg aaccgggagc gcgtcgtcgc gctggccctg cctgttttcg cggccgcgac
      241 ggcgccagcg ccccgggccg cccggggaag gccgcgcgcc accgccatgc cgcctttccc
      301 gactccggag ccggcgaggg ggatgcttcg ggcggcggcc gcggccgtgc cacccttcat
      361 ggcgcctcca ggtcccctgg ccgcgaggcc cgatcacgct tcgacttcgg cgaggcgctg
      421 gtcgctctcc gcctggtcgt tcgtccggag cggcgattcc cgggcgctgg ccgctggcgg
      481 cctgctcggc ggaagccagg ccggcgcccg attcgtctat cgcctcgacc gcgactccgc
      541 ccggccgctc gctgtttcct tccgcctctc ggcgcccctg cggaacgcgc gggcggcgga
      601 ggccgctttg ggcctcgact ggcagccgga ccgccgcctg gcggtccatt tccttgccga
      661 gcggcgtcag gcgctcggcc gggacggacg ctccgctttc tccgtcaccg tccacggcgg
      721 agtcagcgac gccccgctcg gccgcttccg gatcgagtcc tacgcccagg ccgggttggt
      781 cggcacccgc tcacgcgacc cgttcggcga cggctccctg cgcatttcgc tgccggtgga
      841 tgagaaggtc cgcctcggcg ccggagcctg ggccgccgcc cagcccggcc tcgcccgatt
      901 cgacctcggc ccgcaggcga cgctccgcct ccccgtcgcg ggccgagccc tcaccctcgc
      961 cgccgactgg cggctgcgcg tcgccggcaa cgccagaccg ggctcgggcg cgactcttac
     1021 gatgagtacg gactattgaa cttaataatg aattgttctt gatcctataa cgaaagagac
     1081 tatgcgggga cacagcccgg cgagggaggt gcgctacagc cgggaggcgg cgcgcacgct
     1141 gctgcggatc gatcgccgga cctcgcgccg aatccgcgcg aagatcgacc tgctggcggc
     1201 cgaccccggc gcgctcggca acaatgtcgg agcgctcaag ggagaaagcg ggttgaagcg
     1261 cttgaggatc ggaagctggc gcgtgatcta taccgacgat cttaccgtcc tgctcgtgct
     1321 gaagatcgcg ccgcgcgggt cggcctatga ttgaggatga ccgcatgagc tatgagcccc
     1381 aaaccttcgc cgcccccgac ggcaccgaaa tggtgatcct gaaagcggcc gattatgcga
     1441 ggttgaagcg tctctgcgga gaggaagctg aagatgccgc ggagggaatg aaggcactgg
     1501 ccgaaatccg ggatggcatg ggcacgatgc cgggagaggt gctgaattcg atcctcgacg
     1561 agggcctgac gccgctcgcg gcatggcgcc gccatcgcgg cctcagccag tccgcgctcg
     1621 cgggccgggc gggccttagc caggtgtgga tcagccggat cgaacgcggt ggaggttacg
     1681 gatcgcgtga aacgcgtcgc aagctcgccg ccgcgttgga cgcgcctgtt tgggcgctgg
     1741 aggacgagcg gacgacctga cctttcgggc cgcccttccg caccgcgtct ccagcggcta
     1801 gtcagtggcg cgtggacatc tatcttccca tcgccggcct gtcggtcaac gcgctggcga
     1861 taatcctgct cggcggagtg gtcggccttc tcaccggcat ggtcggggtg ggcggcgggt
     1921 tcctgaccac gcccatcctc atcttctacg gaatcccgcc gcccgtcgcg gtcgcctcgg
     1981 cgaccaccca gatcaccggc acaagcgtct cgggggtgct cgcccaccgc cggcgcaagg
     2041 gcgtcgacta caggatgggc gcggtgatca tcgccggcgg cctcctcggg gcgctcgcgg
     2101 gaggcgggct gttccggctg ctccaggacg ccggacagat cgataccgtc atctccttgc
     2161 tctacgtcgt cctgctcggg acgatcggaa tcatgatggc cagggaagcg gcgaccgcgc
     2221 tcgacatctt gaagccgaag gagaattcga agaagcccaa gcgccacaac cccctgatcg
     2281 cggctctccc gatgcgctgg cgcttctacc agtcgggcct ctacatctcg ccgctggcgc
     2341 cgctcctgct cggcttcggc ggcggcatgc tgaccgtgct gctcggagtg ggcggcggct
     2401 tcatcctcgt cccggcgatg atctacctgc tcgggatgtc ggcccaggtg gtggtcggca
     2461 cctccctgct ccagatattg ttcgtcaccg cggcgacgac cctcatccat gccacgacga
     2521 ccaagtcggt cgacatcgtg ctcgccggcc tgctcctcct gggctccgtc atcggcgccc
     2581 agttcggcgc ccgtctcgcc cagaaggcga agcccgaatt gctgcgcatg ttcctcgcca
     2641 tcatcatcct cgccgtcgcg atccgaatgg cgataggcct cggctggcgg cccgaggaaa
     2701 tctacacgct gcaggtgctg tgagcaggct tcgcgccctg ctcctcctgt gcgccgcgcc
     2761 gctcgcgctc ggcgccgcca agccgcggct ggtgcccgac ctgtcgcaga gcagcatcga
     2821 catccagtac agcttcaccg gcgcggagct gcttctgttc ggcgccatcc tctatcccgg
     2881 cggccggacc ccgaccgagc atgccgacat cgcggtggtg gtgaagggcc cggtccagcc
     2941 tttggtggtg cgcgagaagc agaaggtcgc cgggatctgg atgaacgtcg aggcggcgcg
     3001 cttccgctcg gcccccgctt tctacgcggt cgcctcgtcg cggccgctct cgaagctgat
     3061 cgacgagcgc accgccgcga tctacgagct cggcctcgag aacctctcgc tctcccccgg
     3121 cgcgggcaaa tcggccgagg tgcagcgacg cttcgaggcc gggctgatcg acctgatgcg
     3181 gcggcgccgg ctctacagcg aggatcccga cggggtccgg atcaccgacc atgtcctcta
     3241 ccgcggccgg atcaccttcc cgtccctcgt tccggtcggc gactatacgg ctgagacctt
     3301 cctcatcaag gacggccggg tgatcgccgg cgcggttcgc gagatcaagg tgggcaagac
     3361 cggattcgag cgcgaggtcg ccgccgccgc cgaccgctgg tccttcgctt acggccttgc
     3421 cgccgtggcc ctgtcgcttc tgatgggctg gggcggcagc ctggtgctga ggaagggctg
     3481 aatcgtcatc ccggccaagg ccgggatcca tgaacaccga gggtcgaggc cgccaacgtc
     3541 cgtgttcatg ggttccggcc ttcgccggaa tgacgaagaa caggaaaagc agttgtttac
     3601 ccctcgctgc taggaggggc tccgaaaaca gccgggggca gcgacgtccg aatgaccgac
     3661 atgatgaact tcaagaccgt cacccttccc gaggagacgg aggaattcgc gcccaccatc
     3721 cggccgcgca tcccgcagcc ggtgcgccac gcgccgatcg gcaaggtggt cgacattcgc
     3781 ggcggcggcg gccggatcga gctcgaggtg gggcgagtct ccgagatcgc ccgagacccg
     3841 gaccccagca tcgccttgtc cggccaggtc ggcggccacg tcaagctcga agcgggcaac
     3901 cgctggctgc tcgccaacgt ccggagcctc acccttcgcg acggagaggc gggaatgatc
     3961 tcggccgaca tcgacttcct cggcgaaggc gaggaagacg agcagagcgg caagctgatc
     4021 aacttccgcc gcggaatcac cggctacccg ccgccgggcg ccagggcctt cccggtctcg
     4081 ggccaggaca tgcggcagat gttcgccgcc gacgaccgcg cccatatcga gatcggcacc
     4141 gtctatccga ccaaggacgt tcgcggcgcc ctctatgtcg acgccctgct cggcaagcat
     4201 ttcgcccttc tcggctcgac cggcaccggc aagtcgacct cggccgcttt gatcatgcac
     4261 cggatctgcg acctctcgcc cgagggtcac atcgtcatga tcgacccgca cggcgaatat
     4321 tccgccgcct tcaagagcgt cggcgagctg ttcaacgtcg aaaacctggc aatgccctat
     4381 tggctgatga acttcgaaga gcattgcgag gtgttcatca cctccgccgg cgccgaacgc
     4441 cagcgcgaca tggacatcct cgccaaatgc ctcctcctgg cgcgatcgaa gaaccgcgcc
     4501 gccgagggca tgaccaagct caccgtcgat tcgcccattc cctatgcgct ttccgacctc
     4561 gccggggcga tcaacgccga gatggggctt ctcaacaagg cgaccgacac cgcgcccttc
     4621 atgcgcctca agaccaagat tgacgagctg aaggccgatc cccgctacca tttcatgttc
     4681 tcggggatgc tgatcgccga ttcgatgacc agcttcatcg gcaagatctt ccgtctgccg
     4741 gccaggggca agccgatctc gatcgtcgac gtctcggggg tcccctccga catcgtttcc
     4801 gtcgtggtgg ccgtgctttc gcgcctggtc ttcgactatg ccatctggtc gcggaacgaa
     4861 gtgccccggc cggtcctcct cgtctgcgag gaggcgcacc gctacatccc gtcggacaag
     4921 accggcggcg gccagtcggt acgcaagatc ctcgagcgga tcgccaagga aggccggaag
     4981 tacggagtca gcctcggcct catcacccag cggccgtccg acctcgccga gggcgtgctc
     5041 tcgcaatgcg gaacgatcat cgcgatgcgc ctcaacaacg agcgcgacca gcatttcgtc
     5101 aagtcggcga tgccggaggg cgcgcgagga ttcctcgacg tcatcccggc gcttc
//
LOCUS       DASWNU010000266         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_88010, whole genome shotgun sequence.
ACCESSION   DASWNU010000266 DASWNU010000000
VERSION     DASWNU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88010"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VGB57_05225"
     CDS             complement(<1..471)
                     /locus_tag="VGB57_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130787.1"
                     /translation="MRRLLPSSLLGQMLLLIGAALLVAQAVNFAFILNEQQKLSLARN
                     EGPAIGRFTQAAAVIAATAPEARVPLLSAHRMEPAATYRLVPRSAIDREGIERNGEIE
                     QRLASVLREARVPVRAVRAASPMGVARIHEPPRHGHHEARRLLLLAAQLDDGSWL"
     gene            complement(468..1193)
                     /locus_tag="VGB57_05230"
     CDS             complement(468..1193)
                     /locus_tag="VGB57_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0130788.1"
                     /translation="MASTGEPHILLVDDERSIRDPLAQYLARSGVRVTKAKDVAEARQ
                     ALAAYAVDLILLDIMMPGEDGLALCAQVRATSGIPVILLTARAEETDRIVGLEIGADD
                     YVTKPFSPRELLARIKSVLRRSGERPSRLRPADAEAYAFGPWVLRTGERELIDAEGVA
                     VPLSTGEYNLLLALVTHARRILTRDQLLDLAEGRELAAFERSVDNMVSRLRRKIEPDP
                     RNPTLLKTVRGGGYMLAADVRRP"
     gene            complement(1276..1758)
                     /locus_tag="VGB57_05235"
     CDS             complement(1276..1758)
                     /locus_tag="VGB57_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130789.1"
                     /translation="MTSKKKIGLAAAAGALALTAGGATLYAQGSGLRGPMAKFDADSN
                     GAITLDEARAGAARMFEGADSNKDGRASREEMMAFHGRTGDHHKGGHKGGHGGRSGPG
                     GKDGKGPMHLDSDGDGALTLAEAQSGIDVHFARIDSNRDGSIDEAEMRAAHEAHRAGR
                     "
     gene            complement(1905..4325)
                     /locus_tag="VGB57_05240"
     CDS             complement(1905..4325)
                     /locus_tag="VGB57_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0130790.1"
                     /translation="MSQGQVEGDRAEGIAEAALRASEERLRVAQAAGGVGTFEYIPDG
                     ERMLVSPEFCRIWGVDEVEEAPIGFFMALIHPDDRRRVDSTHDERAENALDAIEYRIV
                     RPDTGEIRWLARRGEPIRDSATGAIRWYGVVYDISDRKAAEEALKEREGQLSAFIDQA
                     TAGFAQVDLTGRFTLVNERFCEIAGRTREELLTLRMQEITHPDDLPANVGKFERAVVD
                     GTPYVHEKRYVRPDGSIVWVNNSVSVIRRPSGEPYGILAVTIDVTERRTAEAAVRENE
                     ARLRALTDNLPGGMVYQVSTGRDGKERHFLFVSQSCEKLTGYPAEAILADASIAYGMM
                     EEPEDVLKMAAAEEAAIAEQRPFDIQCRFRRADGVLRWARIISGPRGLPDGTTVWDGI
                     MIDVTDQKRAEEMLRDLNETLEHRVAERAEELALAQEALRQAQKMEAMGQLTGGVAHD
                     FNNLLTPIIGSLDLLHRKALGGEREQRLVQGALQSAERAKTLVQRLLAFARRQPLQPG
                     PVDLPELVRGMVELVASTSGPQIRIETRLDSSLPFARADSNQLEMAILNLAVNARDAM
                     PGGGVLTIGGTAEAVAPGQVAGLAPGRYVRLTVADTGVGMDEATLARAIEPFFSTKGV
                     GRGTGLGLSMVHGLAAQLGGALTLDSRPGEGTVAALWLPVSDSVPASAAVRREPEAGP
                     GTKAGTVLLVDDEPLVRASTADMLADLGYEVVEADSGSDALALVDLRVPDLVITDHLM
                     PGISGTDLALILAAERPGLPVLIVSGYAELDGLPADLPRLTKPFRQADLAACVARLRG
                     GDSRQRHQ"
     gene            4464..5771
                     /locus_tag="VGB57_05245"
     CDS             4464..5771
                     /locus_tag="VGB57_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0130791.1"
                     /translation="MLKRRILAAFSGPGLPIGAVGLPMIVYLPPYYAGTLGLDLALVG
                     LVFFLVRAIDLPLDPLLGHWMDRTRSRFGRYRPWLLAGGLTLAAGVWLVFMAEPGLSA
                     LAAFAFLMVFYLGYSLTAVAQLGWGATLSDDYHQRSRIFSWWMAAQVLGMILVLLVAA
                     GFAAGAPRIHAMGWFVIILAPLTVLLMAAAVPERDRAGERPHASFAELKALARSPLLL
                     RLILLDVLTSVAPGITGAMFLFYFEHRLGFGGEEASLLLLVYFAAGFAAAPLWIGLAR
                     RIGKHRALTSAGVCYATFHFSLSMLPATAGIAVALPAMAIAGLPYIAAPSLLRAMLND
                     AADVDRLETGLDRNALLQALLTSTQKISYAVPVAIIYPVLSLIGFNPKPGGANTEGAI
                     LGMTLLFILAPLILMLASAWVASRWPLDREKHAEVQAALAARG"
BASE COUNT          862 a         2046 c         2092 g         1029 t
ORIGIN      
        1 gagccagctt ccatcgtcga gctgggcggc gaggagcagg aggcgccggg cttcgtgatg
       61 gccgtgacgc ggcggctcat ggatccgggc gactcccatc ggcgaggcgg cgcggaccgc
      121 tcgcaccggc accctggcct cgcgcagcac ggaggcgaga cgctgctcga tctcgccgtt
      181 gcgctcgatc ccttcgcggt cgatcgcgct tcgcggcacc agccggtagg tcgccgcggg
      241 ttccatgcga tgcgcggaca ggagcgggac acgcgcctcc ggcgcggtgg ccgcgatgac
      301 ggcggcggcc tgggtgaagc ggccgatggc gggtccttcg ttgcgggcca ggctcagttt
      361 ctgctgctcg ttgaggatga aggcgaaatt gaccgcctgg gcgacgagca gggcggctcc
      421 gatgagaagc agcatctggc cgagcaggct cgagggaagc agccgcctca cggccggcgg
      481 acgtcggcgg cgagcatgta gccgccgccg cggaccgtct tgagcagggt cggattgcgc
      541 ggatcgggct cgatcttgcg cctcagccgg ctcaccatgt tgtcgacgct gcgctcgaac
      601 gcggcgagtt cgcggccttc ggcgaggtcg agcagctggt cgcgggtgag gatccgccgg
      661 gcgtgggtga ccagggcgag gagcaggtta tactcgccgg ttgagagcgg gacggcgact
      721 ccctcggcgt cgatcagctc gcgctcgccg gtgcgaagca cccacgggcc gaaggcatag
      781 gcctcggcgt cggcggggcg aagccgcgac ggccgctcgc cggagcggcg aagcacggat
      841 ttgatgcggg cgaggagctc gcgggggctg aacggtttgg tgacgtaatc gtcggcgccg
      901 atctccagtc ccactatccg gtccgtttct tccgcccggg cggtgagcag gatgacgggg
      961 atcccgctcg tggcgcggac ctgggcgcaa agggcgaggc cgtcctcgcc cggcatcatg
     1021 atgtcgagga ggatgagatc gaccgcataa gcggcgagcg cctggcgcgc ctcggcgacg
     1081 tccttcgcct tggtgacccg aaccccgctt cgggccagat actgggcgag cgggtcgcgg
     1141 atcgacctct cgtcgtcgac gagcaggata tgaggctctc cggtgctcgc catgggatca
     1201 cattagggac agtcgcgagg gacagtcact agtgactgtc actcgtgtca ctagtgactg
     1261 tcactcgcct ggcgctcagc ggccggcgcg atgggcctcg tgcgcggcgc gcatctcggc
     1321 ttcgtcgatt gagccgtcgc ggttggagtc gatccttgcg aaatggacgt cgatgccgct
     1381 ctgggcctcg gcgagggtga gggcgccgtc gccgtcggaa tcgagatgca tcgggccctt
     1441 gccgtccttg ccgccgggtc cgctcctgcc gccatggccg cccttgtggc cgcctttatg
     1501 atggtcgccc gtccggccgt ggaaggccat catctcctcg cggctggccc ggccgtcctt
     1561 gttcgaatct gcgccctcga acatccgggc ggcgcccgcg cgcgcctcgt cgagggtgat
     1621 cgcgccgttc gagtcggcgt cgaacttcgc catcgggccg cgcaggcccg agccctgggc
     1681 gtagagggtg gcgccccccg ccgtcagcgc gagtgcgccc gcggcggcgg cgaggccgat
     1741 cttcttcttg ctggtcatct gaaatctcct tgtcacgcgc cgatgcggcg ccgggatcca
     1801 catcgcgcgc cattgtcgcg cgcctatccc cgtgggaggg aaaattgtcg caatttgtat
     1861 cagcccccga gtagtgacag tcacggtgac agtcacggtg actgtcactg gtgacgctgg
     1921 cggctgtccc ctcccctcag cctggcgacg caggccgcga ggtcggcctg gcggaagggc
     1981 ttggtgaggc gggggaggtc ggccggaagg ccatccagct cggcatagcc ggagacgatg
     2041 agaacgggca ggccgggccg ctcggcggcg aggatgagag cgaggtcggt gccggagatg
     2101 ccgggcatga gatgatcggt gatgaccagg tcgggcacgc gcaggtcgac cagcgccagc
     2161 gcgtcggagc cggaatcggc ctccaccacc tcatagccga ggtcggcgag catgtcggcg
     2221 gtgctggccc ggacgagcgg ctcgtcgtcg accagcagga cggtgccggc cttggtcccc
     2281 ggccccgctt cgggttcccg gcggacggcg gcggaggccg ggacggaatc ggagaccgga
     2341 agccacaagg cggcgacggt cccctctccc gggcggctgt cgagggtgag agcgccgccc
     2401 agctgagcgg cgagaccgtg caccatcgac aggcccaggc cggtgccgcg gccgacgccc
     2461 ttggtcgaga agaagggctc gatcgcccgg gcgagcgtcg cctcatccat gccgacgccg
     2521 gtatcggcca cggtcagccg gacgtagcgg ccgggggcga ggccggcgac ctgacccggc
     2581 gccacggcct cggccgtccc gccgatggtg aggaccccgc cgccgggcat ggcgtcgcgg
     2641 gcgttcaccg cgaggttgag gatcgccatt tcgagctggt tggaatcggc ccgggcaaaa
     2701 ggcagcgacg agtccaaacg ggtctcgatc cggatctgcg gcccggacgt gctggccacc
     2761 agctcgacca ttccgcgcac cagctcgggc aggtcgaccg gccccggctg aagcggctgg
     2821 cggcgggcga aggcgagcag gcgctggacc agggtcttgg cgcgctcggc ggattgcagc
     2881 gccccttgca ccagcctctg ctcgcgctca ccgcccagcg ccttgcggtg gagcaggtcg
     2941 aggctgccga tgatgggagt gagcagattg ttgaaatcgt gggcgacgcc gccagtgagc
     3001 tggcccatcg cctccatctt ctgagcctgg cgaagcgctt cctgggcgag ggcgagctcc
     3061 tccgcgcgct cggcgacgcg atgctcgagg gtctcgttca ggtcgcggag catctcttcc
     3121 gcccgcttct gatcggtgac gtcgatcatg atcccgtccc agacggtggt gccgtccgga
     3181 aggccgcgcg gcccggaaat gattcgggcc cagcgcaaga ctccgtcggc gcgccggaac
     3241 cggcactgga tatcgaaagg ccgctgctcg gcgattgccg cttcctccgc cgcggccatc
     3301 ttcaagacgt cctccggctc ctccatcatc ccgtaggcta tcgacgcatc ggcgagtatc
     3361 gcctcggccg gatagccggt gagcttctcg cagctctggg agacgaacag gaagtgccgc
     3421 tccttgccgt cccggccggt cgagacctgg tagaccattc cgcccggcag gttgtcggtg
     3481 agggctcgaa gccgcgcctc attctcgcga acggccgctt ccgcggtgcg gcgctcggtg
     3541 acgtcgatgg taaccgcgag gatcccgtat ggctctccgg agggacggcg gatgaccgag
     3601 accgagttgt tcacccagac gatcgacccg tccggacgga cgtagcgctt ctcatgcaca
     3661 tagggcgttc catcgacgac ggcgcgctcg aacttgccga cattggcggg gagatcgtcc
     3721 ggatgggtga tctcctgcat cctgagagtc agcaattcct cgcgggtgcg gccggctatc
     3781 tcgcagaagc gctcgttgac cagggtgaag cggccggtga ggtcgacctg ggcgaaaccg
     3841 gccgtggcct ggtcgatgaa ggcggagagc tggccctccc gctccttgag agcttcctcc
     3901 gcggccttgc ggtcggagat gtcgtagacg acgccgtacc agcggattgc ccccgtcgcc
     3961 gaatctcgta tcggttcgcc gcggcgcgcg agccagcgta tctcgccggt gtcgggacgg
     4021 acgatccgat attcgatcgc gtcgagcgcg ttctccgcgc gctcgtcgtg ggtggaatcg
     4081 acccggcggc gatcgtcggg gtggatgagc gccatgaaaa agccgatcgg cgcctcctcg
     4141 acctcatcga ctccccagat ccggcagaat tccggggaga cgagcatccg ctcgccatcg
     4201 ggaatatatt cgaaggtgcc gaccccgccc gcggcctggg cgacgcgcag gcgctcctcg
     4261 ctggcccgaa gcgccgcttc ggcaattccc tcggcgcggt cgccctcgac ttgcccctgc
     4321 gacacggccc cacgcccttc ctcgtacccg gagcgaacgc cgccgggtga cggcgcgcta
     4381 acacaggcac gagcgtgagt tcaattgctt cgcaggcggt tagaccatag tcctcgacct
     4441 tgcgaacagg gggggaaggc gaaatgctga agcgtcgtat cctggcggcc ttttcgggtc
     4501 cgggcctgcc gatcggcgcg gtcgggctgc cgatgatcgt ctaccttccg ccttattatg
     4561 ccggcacgct cggcctcgac ctcgccctgg tcgggctcgt cttcttcctc gtccgggcga
     4621 tcgaccttcc gctcgacccg ctgctcggcc actggatgga ccggacccga agccgcttcg
     4681 gccgctaccg gccctggctt ctcgccggcg gcctgacgct cgcggccggg gtctggctgg
     4741 tgttcatggc ggagcccggc ctgagcgcgc tggccgcctt cgccttcctg atggtcttct
     4801 acctcggcta ttcgctgacg gcggtggcgc agctcggctg gggcgcgacc ttgtccgacg
     4861 attaccacca gcgctcgcgg atcttctcct ggtggatggc ggcccaggtg ctgggaatga
     4921 tcctcgtcct gctggtcgcg gccggtttcg ccgccggcgc cccccgaatc cacgccatgg
     4981 ggtggttcgt catcatcctc gcgccgctca ccgtgctgct catggcggcg gcggtgccgg
     5041 agcgggaccg ggccggcgag cgcccgcacg ccagcttcgc cgaattgaag gcgctggcca
     5101 gaagcccgct gctccttcgc ctcatcctgc tcgacgtcct gaccagcgtc gccccgggca
     5161 tcaccggagc catgttcctc ttctatttcg agcaccgcct cggcttcggc ggcgaggagg
     5221 cgagcctgct cctgctcgtc tatttcgccg ccggcttcgc cgccgccccg ctctggatcg
     5281 gccttgcccg ccggatcggc aagcatcgcg ccctgacctc cgcgggcgtc tgctacgcca
     5341 ccttccactt cagcctgagc atgttgcccg cgacggcggg cattgcggtc gcgctaccgg
     5401 cgatggcgat cgccggcctg ccctacatcg ccgctccgtc gctgcttcgg gcgatgctca
     5461 acgacgccgc cgacgtcgac cgcctcgaga ccggcctcga ccgcaacgcc ctgctccagg
     5521 ccctgctcac cagcacccag aagatcagct acgcggtgcc ggtggcgatc atctatcccg
     5581 tactgagcct gatcggcttc aacccgaagc ctggcggagc caataccgaa ggcgccatcc
     5641 tggggatgac ccttctcttc atccttgccc cgctcatcct gatgctcgcc tccgcctggg
     5701 tcgcctcccg ctggccgctg gaccgggaga agcatgccga ggtgcaggcg gcgctggcgg
     5761 cgcgagggtg aattccatcc ttcctctcct gctttccgcg ggagaggggg gatccaggct
     5821 gaagcgacat ttccccttcc aaccgccgtc gaacatagct caagttaaac ttgagcaact
     5881 actcgtctaa accaggttaa tttcgctgct gaaacgggaa cccgcgcaat ctcaggctgt
     5941 tactaaccgt acgcctgacg ctgcaaaggt ctttgcggca attacgaaac tattgctgag
     6001 gtggttccat gaactctctt gagttgtgg
//
LOCUS       DASWNU010000267         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_139004, whole genome shotgun sequence.
ACCESSION   DASWNU010000267 DASWNU010000000
VERSION     DASWNU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139004"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGB57_05250"
     CDS             <1..410
                     /locus_tag="VGB57_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0130792.1"
                     /translation="ILTRVTQARAEATRVTVSPEQLSDPAAMQRYADAQNRLSASIIP
                     LQRLQEAYPTLQSQANFGTLMSQLESTENRINITIRDYNTAVQGYNTRIRTFPDVIGA
                     KVIYGAKPMATFQATTPGADQAPEVNFDTNTGA"
     gene            418..984
                     /locus_tag="VGB57_05255"
     CDS             418..984
                     /locus_tag="VGB57_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0130793.1"
                     /translation="MIRTFLLLPLLVPGSAACDTQAPARAAQAQPPIVQVSSADALPA
                     LTGRVVDEAGVLTAPAEAELSARLAALEKETTDQLVVVTVGSLRSEPIEALGLRLGNG
                     WGIGRKGVDNGVLLIVAPSDRRARIEVGKGLQGLITDKRAQRIMDEQVVPACREGHCD
                     RAIGDGVAAIAKLLRSDPKRPQRRRGAA"
     gene            984..1775
                     /locus_tag="VGB57_05260"
     CDS             984..1775
                     /locus_tag="VGB57_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0130794.1"
                     /translation="MRGLFLFLLLFLAAPAAAQDFPKLTGRVVDTANIIPPADEAALT
                     QKLEAVETASTRQLVVATVPSLQGYDIADYGYQLGRTWGIGQKGANNGIILLVAPNER
                     KVRIDVGYGLEPIMTDALSHQIITQQILPAFKRNDYPGGINAGADAIIAQLQAPPEQA
                     EQKALAAQQAQRERGGDSEGGGSIFPLIFWGIVFVFVILPMLRGGRRGRRHRRSGVFV
                     WGPGLGGGGWGGGSSGGGFGGFGGGGGGFGGGGGSFGGGGASGGW"
BASE COUNT          313 a          659 c          659 g          265 t
ORIGIN      
        1 ggatcctgac ccgggtcacc caggctcggg cggaagccac aagggtcacc gtcagcccgg
       61 agcagctctc cgatcccgcg gcgatgcagc gttacgccga cgcccagaac cggctgagcg
      121 cttcgatcat cccgcttcag cggctccagg aggcctatcc gacgcttcag agccaggcca
      181 atttcggaac cctgatgagc cagctcgaaa gcaccgagaa ccggatcaac atcaccatcc
      241 gcgactacaa caccgccgtt cagggctata acacccgaat ccgcaccttc cccgacgtga
      301 tcggcgccaa ggtgatctac ggggcgaagc cgatggcgac cttccaggcg acgactccgg
      361 gcgccgacca ggcgcctgaa gtcaatttcg acacgaacac cggcgcctga ccggccggtg
      421 atccggactt tcctcctcct gccgctgctc gttccgggca gcgccgcgtg cgacacgcag
      481 gcgccggcac gcgccgccca ggcgcagccg ccgatcgtgc aggtttcctc ggccgatgcc
      541 ttgccggcgc tgacggggcg agtggtcgac gaggccggcg tcctgaccgc gccggcggaa
      601 gcggagctca gcgcgcgtct cgcggccctg gagaaggaga ccaccgatca gctcgtcgtg
      661 gtgacggtcg gcagcctccg gagcgagccg atcgaggcac tcggcctgcg tctcggcaac
      721 ggctggggga tcgggcggaa gggcgtcgac aacggtgtgc ttctgatcgt cgcgccctcg
      781 gaccggcggg cgaggatcga ggtcggcaag ggtcttcaag gcctcatcac cgacaagcgc
      841 gcccaaagga tcatggacga gcaggtggtc cccgcgtgcc gcgaaggaca ttgcgatcgc
      901 gccatcgggg acggagtcgc cgccatcgcg aagcttctgc ggagcgatcc caagcggccg
      961 cagcgcaggc gcggggccgc ctgatgcggg gcctgttcct cttcctcctg ctgttcctgg
     1021 cggcgcccgc ggcggctcag gatttcccga agctgacggg gcgggtcgtc gatacagcca
     1081 acatcatccc tcccgccgac gaggcggcgc tgacgcagaa gctggaggcg gtcgagaccg
     1141 ccagcacccg tcagctggtc gtcgcgacgg tgccgagcct gcagggctac gacatcgccg
     1201 attacggcta ccagctcggc cggacctggg ggatcggcca gaaaggcgcg aataacggaa
     1261 tcatacttct cgtcgctccc aacgagcgca aggtgaggat cgacgtcggc tacggcctgg
     1321 agccgatcat gaccgacgcc ctctcccatc agatcatcac ccagcagatc ctccccgcct
     1381 tcaagcggaa cgactatccc ggcggaatca acgccggcgc cgacgccatc atcgcgcaat
     1441 tgcaggcacc gcccgagcag gcggagcaga aggcgctcgc cgcccagcag gcgcagcggg
     1501 aacgcggcgg cgacagcgag ggcggcggct cgatcttccc gctgatcttc tggggaatcg
     1561 tcttcgtctt cgtcatcctg ccgatgctcc gcggcggccg gcgcgggcgg cgccaccgcc
     1621 gctcgggcgt cttcgtgtgg ggaccggggc tgggcggagg cggctggggc ggcggctcca
     1681 gcggcggcgg cttcggaggc ttcggcgggg gcggaggcgg cttcggcggc ggcggcggct
     1741 cgttcggcgg cggcggagcg tcgggagggt ggtgatgccg aggttcagcg aggccgacca
     1801 taagctcgtc accgacgcgg tggccaaagc cgagcgctcc agcgacggcg agatcgtcac
     1861 catcgtcgct ccgcgctccg acgcctatca cgacgt
//
LOCUS       DASWNU010000268         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_243872, whole genome shotgun sequence.
ACCESSION   DASWNU010000268 DASWNU010000000
VERSION     DASWNU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243872"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..568)
                     /locus_tag="VGB57_05265"
     CDS             complement(17..568)
                     /locus_tag="VGB57_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0130795.1"
                     /translation="MFVRTERLLLRPGWSQDAPALFQAIADERIVRNLATAPWPYRLG
                     DAESFLATERRPTEASLLIFLRSGGEAGLIGGIGFGRRPTGRIEFGYWIARRHWGRGY
                     ATEAGRAVLAMARHSLRLKGLDAGHFLDNPASGRVLEKLGFRPTGKIVPHYSAGRGEA
                     APCRRLELEFGEEAEIAPAAMAA"
     gene            complement(745..1020)
                     /gene="rpmA"
                     /locus_tag="VGB57_05270"
     CDS             complement(745..1020)
                     /gene="rpmA"
                     /locus_tag="VGB57_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY0130796.1"
                     /translation="MAHKKAGGSSRNGRDSAGRRLGVKKFGGETVLAGNIIVRQRGTR
                     WYPGANVGIGKDHTLFALTHGRVSFRDGKLGRKFVCVDMPQTTEAAE"
     gene            complement(1047..1661)
                     /gene="rplU"
                     /locus_tag="VGB57_05275"
     CDS             complement(1047..1661)
                     /gene="rplU"
                     /locus_tag="VGB57_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0130797.1"
                     /translation="MFAVVRTGGKQYRVAAGDKIVVEKLAGEAGDTSRLDDVLLAGEG
                     ADQRSTDGLTVSAEIIAQAKADKVIIFKKRRRHNYRRKKGHRQQHTILRITAIGDQQA
                     ETPKKAKAEKPADEAPAAAAAAPAEAPAAAAPAAEAPAAEAPKKSRAKKAPEESGAPE
                     APAATEAQPQAPLKVDGAESEMTDSPVAAKKKASPKKPAADKDA"
BASE COUNT          252 a          628 c          642 g          337 t
ORIGIN      
        1 cgcatgggga ggcaaatcag gccgccatcg ccgccggggc gatctccgcc tcctcaccga
       61 actccagctc gagtcgccgg cagggcgccg cctcgccgcg accggcgctg tagtggggga
      121 cgatcttccc ggtcggcctg aagcccagct tctcgagcac ccggccggag gcgggattgt
      181 cgaggaagtg accggcgtcg agccccttca gccgcaggct gtggcgcgcc atggccagga
      241 ccgcccgccc agcctcggtg gcgtagccgc gtccccaatg gcgccgcgcg atccagtagc
      301 cgaactcgat cctgcccgtc ggccgacggc cgaagccgat cccgccgatc aaccccgcct
      361 cgccgccgga gcggaggaag atgagcagcg aggcctccgt cggccttcgc tcggtcgcaa
      421 ggaaggattc ggcgtccccc aggcgataag gccagggcgc ggtggcgagg ttgcggacga
      481 tgcgctcgtc ggcgatcgcc tggaacaggg caggggcgtc ctggctccag ccgggccgca
      541 gcaacagtct ttcggttctc acgaacatcg tctcgtcctc ccttgccgcc gccgggccgt
      601 acccgcgagg ggtgacggtt cggcggccgt ttcgagggag aacgcaaaaa ggagagaaac
      661 ccggcggctc ctctccctcg aattcggttc tctattgaga cctggagccg cccctcctgg
      721 ggacggccct tcctcggctc gtccctattc cgctgcttcg gtcgtttgcg gcatgtcgac
      781 gcagacgaac ttgcggccga gtttcccgtc gcgaaacgag acccggccgt gggtcagcgc
      841 aaaaagggta tgatccttgc cgattcccac gttggcgccg gggtaccagc gggtgccgcg
      901 ctgacgcacg atgatgttgc cggcgagcac ggtctctccg ccgaacttct tgacgcccag
      961 gcgacggccc gcgctgtcgc gaccgttgcg tgaggatccg cctgctttct tatgtgccat
     1021 gactaagctc gatatctctc tagggtttat gcgtctttat cagcggcggg cttctttggg
     1081 gaagcctttt tcttcgccgc gaccgggctg tccgtcatct cgctctcggc accgtccact
     1141 ttgagcggag cctggggctg agcttcggtg gccgcgggag cctcgggcgc gccggattct
     1201 tcgggggcct tcttcgccct cgacttcttc ggagcctcgg cggcgggcgc ctcggcggcc
     1261 ggagcggcgg cggcgggagc ctcggccgga gcggcggcgg ccgcggcggg agcctcgtcg
     1321 gcgggcttct cggccttggc cttcttcggc gtctcggcct gctggtcgcc gatggcggtg
     1381 atcctcagga tcgtgtgctg ctggcgatgg cccttcttgc gacggtaatt gtgccggcgg
     1441 cgcttcttga agatgatcac cttgtcggcc ttggcctggg cgatgatctc ggccgagacg
     1501 gtgagcccgt cggtggaacg ctggtcagcg ccctcgccgg cgagcaggac gtcgtccagc
     1561 ctactggtgt cgcccgcctc tccggcgagc ttctcgacga cgatcttgtc tccggcggcg
     1621 acgcgatact gcttgccgcc cgtgcgcacg actgcgaaca tggctttttc tcaactcaaa
     1681 tgcatgtgcc cgccggggac gacccccgcg ggaggaaggg cgcagctagg tcaggagacg
     1741 gccaaagtca acattgaggt cgatccggcc gccccggagg gaagccgtca gggcgccgga
     1801 tagcaattgc ggtaattgcc gcctgtcgcg caggccggcg gcattggcgg ctggtcgtc
//
LOCUS       DASWNU010000269         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_139250, whole genome shotgun sequence.
ACCESSION   DASWNU010000269 DASWNU010000000
VERSION     DASWNU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139250"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGB57_05280"
     CDS             <1..528
                     /locus_tag="VGB57_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY0130798.1"
                     /translation="ERWLEAQPAGCAGLWIKFAKAGSGLASVTKREAIDAALCHGWID
                     GQLDRYDDRHWLIRFTPRKARSKWSQVNRARATELLEAGRMRPGGLAQIEAAKADGRW
                     EAAYAPASKAEVPADLQAALDRSPAAATFFATLTGANRYAILYRIGAVKKAETRARKI
                     AEFVAMLERGETVHG"
     gene            798..1634
                     /locus_tag="VGB57_05285"
     CDS             798..1634
                     /locus_tag="VGB57_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130799.1"
                     /translation="MARLSITTAWNESATFLKLHFGALFTIALAFVALPNVAMQALGP
                     AAATPGETPEPGLWLLLIPLVLVLNVAASLAMSSLALGRMNVVGEAIAHGFRRFLPML
                     GAIAILIVAMCVVFVPLVMLSGLTPDDFTRPTPANSGRLLLVMLLVMGVGLFFAVRLL
                     LMTPVAAAERAGSIAIIRRSWNLTAGHFWKLLGFLLLMLIVLLVVTMVATMILGLLIA
                     AVAGPPLPGTTGGFVLALVSSLVNAAFVVVTTTLIARIYVQLSGAGPAGSAAAGGATS
                     GS"
     gene            complement(1565..2182)
                     /locus_tag="VGB57_05290"
     CDS             complement(1565..2182)
                     /locus_tag="VGB57_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0130800.1"
                     /translation="MLTPLPPGHVGAVVTYLETTQRPRPSALPASPLRLERWRSPEPA
                     RYRLLFERVGARWLWYSRLAMDDERLRSEMAEVHAVVDRAGIEVGMIELDFRAPEQCL
                     IRFLGLVPELAGKGHGKWLLARTLALAWRSGVRRVCVNTCTLDHPAALKAYLNAGFTA
                     VKRAFESFPDPRLAGLLPADSAPQLPLVAPPAAAEPAGPAPESWT"
BASE COUNT          331 a          768 c          806 g          353 t
ORIGIN      
        1 gagcgctggc tcgaggcgca gcccgcgggc tgcgcggggc tgtggatcaa gttcgccaag
       61 gcgggctccg gccttgccag cgtgacgaag cgggaggcga tcgatgccgc gctttgccac
      121 ggctggatcg acggccagct cgacagatat gacgacaggc actggctcat ccgcttcacg
      181 ccccgcaagg cgcgaagcaa atggtcgcag gtgaaccgcg cccgggccac cgagctcctc
      241 gaggccggcc ggatgcgccc gggcgggctg gcgcagatcg aggccgccaa ggctgacggc
      301 cggtgggagg cggcctatgc cccggcgagc aaggccgagg tgccggccga cctccaggcc
      361 gccctggacc ggagcccggc ggcggcgacc ttcttcgcga ccctgaccgg cgccaaccgc
      421 tacgcgatcc tctaccgaat aggcgccgtg aagaaggcgg agacgcgggc gcgcaagatc
      481 gcggagttcg tcgccatgct ggagcggggg gagacggtgc atgggtgagg gccggcggga
      541 actggcgttt ggcgtttgcg tcggcgactg gatggaaaag tgacagtcac ggtgactgtc
      601 accgggtgga aaagtgacag tcacggtgac tgtcgccaag ctccctgtcc aaccggcatt
      661 cacgcccgga tgctcgacat attaggtatc gaactggacc aggcggagat tccgggccgg
      721 cggccgccct gggcaagata gaagattgcc cggcgcccgg cgctggccta atcggcttgc
      781 gcaagagggg aaaggccatg gccagattgt cgatcacaac tgcctggaac gagagcgcca
      841 cgttcctgaa gctccatttc ggagcgctgt tcacgatcgc gctggcgttc gtcgcgcttc
      901 ccaacgtcgc catgcaggcg ctcggcccgg ccgccgccac gcccggcgag actccagagc
      961 cgggcctctg gctgctgctc atccctctgg tgctggtcct gaacgtcgcc gcctccctcg
     1021 ccatgtcgtc gctggcgctc ggccggatga acgtcgtcgg cgaggcgatc gcccacggct
     1081 tccggcgctt cctgccgatg ctcggggcga tcgcgatcct gatcgtggcg atgtgcgtcg
     1141 tcttcgtgcc gctggtcatg ctgtcaggcc tgacgccgga cgatttcacc cggcccaccc
     1201 ccgccaattc cggaaggctg ctcctggtca tgctcctcgt catgggcgtt ggcctgttct
     1261 tcgcggtccg gctgctcctc atgaccccgg tggccgccgc cgagcgggcc ggctcgatcg
     1321 ccatcatccg ccggagctgg aacctcaccg ccggccattt ctggaagctg ctcggcttcc
     1381 tcctcctcat gctgatcgtc ctcctcgtgg tgacgatggt cgccaccatg atcctcggcc
     1441 tgctcatcgc cgccgtcgcc gggccgccgc tgcccgggac caccggcggc ttcgtcctcg
     1501 ccctcgtcag ctcgctggtc aacgccgcct tcgtcgtcgt gacgacgacg ctcatcgccc
     1561 ggatctacgt ccagctctcc ggcgccgggc cggcgggctc ggcggcggcc ggcggagcga
     1621 ccagcggcag ctgaggcgcc gaatcggccg gcaacagccc ggcgaggcgc gggtccggaa
     1681 agctctcgaa ggcccgcttc accgctgtga agccggcgtt gagataggcc ttgagcgccg
     1741 ccggatggtc gagcgtgcag gtgttgacgc agactcgccg gacgccggag cgccaggcga
     1801 gcgccagggt ccgggcgagc agccatttgc catggccctt gccggcaagc tcggggacga
     1861 gcccgaggaa gcggatcagg cattgctccg gggcgcggaa gtcgagctcg atcattccca
     1921 cctcgatccc cgcccggtcg accacggcat ggacctcggc catttcgctg cgcagccttt
     1981 cgtcgtccat ggcgaggcgc gaataccaga gccagcgggc gccgacgcgc tcgaagagga
     2041 gtcggtagcg ggcgggctcg ggcgagcgcc agcgttcgag gcgcaggggc gaggcgggga
     2101 gggcgctcgg gcgggggcgc tgcgtcgtct ccaggtaggt gacgacggcg ccgacatggc
     2161 ccggagggag gggggtcagc acctcgagtc tcgattgctg ccggctaggc tgctcgtttc
     2221 agggcgatga cctcggaaat ggcccgggag agatcccg
//
LOCUS       DASWNU010000270         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_61416, whole genome shotgun sequence.
ACCESSION   DASWNU010000270 DASWNU010000000
VERSION     DASWNU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61416"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VGB57_05295"
     CDS             <1..700
                     /locus_tag="VGB57_05295"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY0130801.1"
                     /translation="IGRAGILPGGLNVKRRAPGLHKLLLDRAERTLSDPLTVLDWVNL
                     WALAVNEENAAGGRVVTAPTNGAAGIVPAILRYYQRFTPHSTREGVRTFLLTAAAVGS
                     LFKENASISGAEVGCQGEVGVACSMAAAGLTAALGGTNWQIENAAEIGMEHNLGLTCD
                     PVGGLVQVPCIERNAVGAIKAIEASRLALVGTGDHLVSLDQVIETMRKTGLDMNERYK
                     ETSLGGLAVNVVEC"
     gene            851..>1821
                     /locus_tag="VGB57_05300"
     CDS             851..>1821
                     /locus_tag="VGB57_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0130802.1"
                     /translation="MRLRLSLLAAAAFLAAPAFAEVEKPAAIVADGIPAVPDDLAERT
                     RPYMEFRAAGLQGWNPARRSLAIATRFGNTAQVHEVSRPLGMRRQITFEADAIAGASY
                     ARKLGDVLVIQKDVGGSEFWQLYVLDNGRLRMITDGKSRNEINAWSHDGRWLAYTSTR
                     RNGTDNDIWIVDPRNPASNRMLAQVKGGGWALTDFAPDGRSAIVAEYLSVNKTNLYRV
                     DVATGRMTPIGNHQAPISYGGAQFAPNGTLWVTSDEGSDFQRLGTLNPATGRFTAMAP
                     ETRWDVENFDISDDGRFIAYTVNEAGISRLRLLDARTRRSRAVAGLPV"
BASE COUNT          291 a          712 c          554 g          264 t
ORIGIN      
        1 catcggccgt gccgggatcc tgcccggcgg gctcaacgtc aagcgccggg cccccggcct
       61 ccacaagctc ctcctcgacc gggccgagcg cacgctttcc gacccgctca ccgtgctcga
      121 ttgggtcaat ctctgggcgc tcgcggtcaa cgaggagaat gccgccggcg gccgggtggt
      181 caccgcgccc accaacggcg ccgccgggat cgtccccgcc atccttcgct actaccagcg
      241 cttcaccccc cattcgacga gggaaggcgt gcgcaccttc ctcctcaccg ccgcggcggt
      301 cggctccctg ttcaaggaga acgcctccat ctccggcgcc gaggtcggct gccagggcga
      361 ggtcggggtg gcctgctcga tggccgcggc cggcctgacg gcggcgctcg gcggcaccaa
      421 ctggcagatc gagaatgccg ccgagatcgg catggagcat aatctcggcc tgacctgcga
      481 cccggtcgga ggactcgtcc aggtcccctg catcgagcgc aacgcggtcg gggcgatcaa
      541 ggcgatcgaa gcctcccgcc tcgccctcgt cggcaccggc gatcatctcg tcagcctcga
      601 ccaggtgatc gagacgatgc gcaagaccgg cctcgacatg aacgagcgct acaaggaaac
      661 ctcgctcggc ggcctcgcgg tcaacgtggt ggaatgctga aaaccgtcat cccggcgaag
      721 gccgggatcc atgaacaccg tcgccgttcg ccctgacgcg tccgtgttca tggacggtgg
      781 acgaccaacg tcttgcccgc tccgcccgcg catcctagcg tccgccccgc cttccgcccg
      841 aggagtcccc atgcgccttc gcctttccct gctcgccgcc gccgcctttc tcgccgctcc
      901 cgccttcgcc gaggtggaga agccggccgc catcgtcgcc gacgggatcc ccgccgttcc
      961 cgacgatctc gccgagcgca cccgccccta tatggagttc cgagccgccg gccttcaggg
     1021 ctggaacccg gccaggcgct cgctcgccat cgccacccgc ttcggcaaca ccgcccaggt
     1081 ccacgaggtc tcccggccgc tcggcatgcg ccgccagatc accttcgagg ccgacgcgat
     1141 cgccggcgcc tcctatgccc gcaagctggg cgacgtgctc gtcatccaga aggacgtcgg
     1201 cggaagcgag ttctggcagc tctacgtcct ggacaacggc cggctgagga tgatcaccga
     1261 cggcaagagc cgcaacgaga tcaacgcctg gagccacgac ggccgctggc tcgcctacac
     1321 ctccacccgc cgcaacggca ccgacaacga catctggatc gtcgatccgc gcaacccggc
     1381 cagcaaccgg atgctcgccc aggtcaaggg cggcggctgg gccctgaccg atttcgcgcc
     1441 cgacggccgg agcgcgatcg tcgccgaata tctctcggtc aacaagacca acctctaccg
     1501 ggtcgacgtc gccaccggcc ggatgactcc gatcggcaat caccaggcgc cgatctccta
     1561 cggcggcgcc cagttcgccc ccaacggcac cttgtgggtc acttcggacg agggctcgga
     1621 cttccagcgc ctcggaaccc tcaacccggc gacgggccgg ttcaccgcga tggcgcccga
     1681 aacccgctgg gacgtcgaga atttcgacat ctccgacgac ggccgcttca tcgcctacac
     1741 cgtcaacgag gccgggatca gccgcctcag gcttctcgac gcccgcaccc gaaggagccg
     1801 cgccgtcgcg ggccttccgg t
//
LOCUS       DASWNU010000271         9188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_503349, whole genome shotgun sequence.
ACCESSION   DASWNU010000271 DASWNU010000000
VERSION     DASWNU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503349"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGB57_05305"
     CDS             complement(<1..602)
                     /locus_tag="VGB57_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130803.1"
                     /translation="MTRNMRLLASASVLAATAFAASPAFAAGTTAGTTVTNSVTLDYK
                     VAGIDQAQVSASDSFTVDRKVNLVVSESGGTATSVTPGQTAAVTIFSVTNSSNAPLDF
                     ALAASQLTGGTAPHTGTDSFNVTNAKIYADTNANGGYDPGTDQETAYLDQVAADTSRT
                     VFIVSDIPLGRANGDVAVVRLTATAAEATSSGTLGATVTQT"
     gene            complement(671..1132)
                     /locus_tag="VGB57_05310"
     CDS             complement(671..1132)
                     /locus_tag="VGB57_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130804.1"
                     /translation="MKIWLALFALLAPAAASAADNVSLTSKVLVERVKPGPDGKPVTV
                     REEPGVVTPGDRLVVVLSYRNAGAQPATGFTLTNPIPASVAFTGTDDSSAAVSVDGGK
                     SWGALASLKVVAADGTSRPAVAADVTHIRWSLGQPIAAGSGGELSFRGVVE"
     gene            complement(1147..2160)
                     /locus_tag="VGB57_05315"
     CDS             complement(1147..2160)
                     /locus_tag="VGB57_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130805.1"
                     /translation="MRYQATNAKSPAFRKNAALAALTAVSACVFASPAQAAGTPAGTD
                     ITNVATATYELPNGDEASVDSNIVTLKVDELLDVGVAWSDPSDVTASAGGTGQRLKFT
                     ITNGGNGAESFTLATVANGGGDDFDPSVTSIVLDSNSNGAYDAGVDTVYASGSNDPQL
                     DPDQSITVFVLSSIPAGAGDGQRGRVDLTAVAKTGSGAPGTSFAGQGQGGGNAVVGAT
                     GADSDDDGYYRIARASVAFLKSATVADAFGGTAAAPGSTITYTLAATVSGTGDLANLR
                     VSDPVPAGTTYKPGSLTLEGAPLTDSADADQGSFTGTAVTVGLGTVAAGATKTITFQV
                     KID"
     gene            2281..3489
                     /locus_tag="VGB57_05320"
     CDS             2281..3489
                     /locus_tag="VGB57_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0130806.1"
                     /translation="MNKLQGKEVIEGATQVEPVDPRWEPEAVAYRRDRLLAALTLIAG
                     AGLAIALPFALRAGAEFFLPVTAALVIAVALVPMLEWLERRRVPSPLAAFTCILVLIT
                     MANIAVASIVLPAAEWVRLLPERIGRVRDTLAPLMEVYSDLERFIDDIVEQFGSHAAA
                     PRTVQLETPNSLLDIIATSAPFAAIQMFFALLVIFFFLAGWTSMRRRTITARSSFDGA
                     MTTARAIQEVVDATSTYLGTITIVNIAMGLTVAFILYLLDMPTPLMWGGIVAVLNYIP
                     YLGPIASVLLLALGGLMAFADPWYAFLPAICFIGIHLVEANLITPFFVGRRLTINPLL
                     ILVALSFWAWVWGTTGALLAVPLLIITKTVLDAAGKPDIAGFLFEEGTLTGIHHEEHG
                     EADRAAAAKP"
     gene            3537..3613
                     /locus_tag="VGB57_05325"
     tRNA            3537..3613
                     /locus_tag="VGB57_05325"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3571..3573,aa:Asp,seq:gtc)
     gene            3657..4388
                     /locus_tag="VGB57_05330"
     CDS             3657..4388
                     /locus_tag="VGB57_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0130807.1"
                     /translation="MASRYDRIGLNYSNLRKPDPRIAARIEAALGDCRTILNVGAGTG
                     SYEPMGREVTALEPSIEMIRQRSAPAAAVVQGRAEALPFADRSFDAAMAVLTIHHWSG
                     QARGLAEMRRVTNGPIVLLTYDPAFRGFWLADYIPGLVALDEAQMPSMRDYQRWLGPV
                     AISPVPIPHDCTDGFLSAYWRRPAAYLDPRVRAAMSSFWALGDVSEALARLEADLESG
                     AWARRYAELLALDACDCGYRLVVAR"
     gene            4458..5666
                     /locus_tag="VGB57_05335"
     CDS             4458..5666
                     /locus_tag="VGB57_05335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0130808.1"
                     /translation="MPRRLAAFLLLAALPGPAPAAGERAFEAALSDFRKLHRAETRRS
                     AIAGSSFYMVRDGRTVVADHLGEQDSEARVPVDARTIYHWASITKTMTGIAILQLRDR
                     GLLKLDDPITRFVPELAAVHNPFGDTNAITLRQLMSHSAGFRGGTWPWREHEWQPFEP
                     RSWSQLAAMLPYTRVEFRPGSRFGYSNPGIVYLGQVIERLSGEDFEVYVDKNILKPLG
                     MHSSYFDRTPPHLLPHRSRSYYIRDGKRAAAPFDVDTGVTVSNGGLNAPMPDMARYVA
                     FLLGDPARAGEYDLVLKRASLEEMWRPQISAGEDFTQGRMAKTTQSGLSFFIDRGRGV
                     RFVGHNGDQNGFRAYLSLCPDQRLGTLLAFNTETREVRNDPSNRGTAESRIALATDSL
                     CEALAKSGPA"
     gene            5795..5968
                     /locus_tag="VGB57_05340"
     CDS             5795..5968
                     /locus_tag="VGB57_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130809.1"
                     /translation="MTGRAQEKDDQAEAEPLDPPFAERCRESLADPRPDIPAAEVRAY
                     LEALDESRLKRGA"
     gene            complement(6217..8088)
                     /locus_tag="VGB57_05345"
     CDS             complement(6217..8088)
                     /locus_tag="VGB57_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY0130810.1"
                     /translation="MRRTYWLTAAAALAGAVPVTAAVQAQAPAATAASEDTRLYAFLD
                     QEFAEELRQRPQLATQLGLKEGQDRLDDIDEAAALRRLEWRRASVARMKSQFDRSKLS
                     ASARTNYDIWALELDRAELSYRYRQYSPALYSFLYSAHSRLPDFLINTHNVQDAGDMR
                     AYAARLRAIPAVLDEAIVQSRASDAIGVRAPRFQVERVIAGSRAIVTGAPFDAGPPSP
                     LWADAQAKVAKLRTGGKVTPAEAEALLEETRTALLSIRPAYERVVAWGQSALAAAPSG
                     RVGAISLPGGADYYAAALKLNTTTDLNAEQIHRIGLSEVKRIEGEQDALARQAGFKDR
                     AAYYADRERRNPPRPWTDALRSEYLRKANEAIARTRTLLPKYFNQLPAHRMEVVREPS
                     FSEVAGGAAHAAAPSPDGTRPGRVYVHLLGVNSDPAVDAIPDLMCHEGVPGHVMQGDI
                     QVRQKTGPKFRQSARYVAFGEGWALYAEALCKEMVAYPDVASDFMRLEAELFRAARLV
                     VDTGIHAKGWTEDRAVDYMVATGRASPDQARSEVRRYITLPGQATGYKIGMLKIMELR
                     RKAERELGPRFDIRAFHDLLIGSGSLPLSILEREVDGWIAGRAAPAPSERRGRGRRT"
     gene            complement(8321..8830)
                     /locus_tag="VGB57_05350"
     CDS             complement(8321..8830)
                     /locus_tag="VGB57_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130811.1"
                     /translation="MAEVRRGRSSGPGAGALIPNPALKPLEFLIGEWRTTGTHPLVPG
                     ETLCGRTVFAPHEGGAFLIMRSQVDHPQFPDGVAIIGSDGACGDLTMIYFDEREVSRI
                     LDVTAADRSVTWRHDDAEFAQSLTIAADGDRLVSKGRMSKKGGAWEDDLSQVYTREAP
                     WNEPPARSP"
     gene            complement(8818..9090)
                     /locus_tag="VGB57_05355"
     CDS             complement(8818..9090)
                     /locus_tag="VGB57_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEY0130812.1"
                     /translation="MGRPPRRHRHAARVDRLPVNAPRPSWRKPAGMLLLLLLIAVWGF
                     VVVTVAEALPELPWPLKALFFIAAGIVWILPLKPLLRWMELGRWRK"
     gene            complement(9033..>9188)
                     /locus_tag="VGB57_05360"
     CDS             complement(9033..>9188)
                     /locus_tag="VGB57_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0130813.1"
                     /translation="DRALSHLREGGQIFTIGLAWELQILADPIPADPWDVPLDAIATP
                     REWIDCR"
BASE COUNT         1385 a         3120 c         3094 g         1589 t
ORIGIN      
        1 gtctgggtca ccgtcgctcc gagcgttccg gaagaggtgg cttccgcggc cgtggcggtg
       61 aggcgaacga cggcgacgtc gccgttcgcc cggccgagcg gtatatcgga gacgatgaag
      121 accgtccggc tggtatcggc cgcgacctgg tcgagatagg ccgtctcctg atcggtgccc
      181 ggatcatagc cgccgttggc attggtatcg gcatagatct tggcgttggt gacgttgaaa
      241 ctgtcggttc cggtatgcgg agcggtcccg ccggtcagct ggctcgcggc gagcgcgaag
      301 tcgagcggag cgttggaaga gttggtgacg ctgaagatcg tcaccgccgc cgtctgcccc
      361 ggcgtgacgc tggtcgcggt gccgcccgac tcgctcacca ccagattgac cttgcgatcg
      421 accgtgaagg agtcggaggc gctgacctgg gcctggtcga tcccggcgac cttgtagtcg
      481 agggtgacgc tgttggtgac ggtggtaccc gcggtcgtcc cggccgcgaa ggcgggggac
      541 gccgcaaaag ccgtcgccgc cagcacgctg gcggaggcga gcaatcgcat gtttctggtc
      601 atctctggtc caatcgtgtt tgcgtggacg ccgaaatccc ccccgccggc gcccgatgcg
      661 ggttacgaaa ttattcgacg acgccccgga agctgagctc cccgccgctg cccgcggcga
      721 tcggctggcc gagcgaccag cggatgtggg tgacgtcggc cgcgaccgcc gggcggctgg
      781 tgccgtccgc cgccacgacc ttgagtgacg ccagcgcccc ccagctcttg ccgccgtcca
      841 ctgaaacggc agcgctcgaa tcgtcggtgc cggtaaaggc gaccgatgcc gggatcggat
      901 tggtgagagt gaagccggtc gccggctggg ctccggcgtt gcgatagctg aggacgacga
      961 cgagcctgtc ccccggagtc accacgccgg gctcctcgcg gaccgtgacg ggcttgccgt
     1021 cggggccggg cttgacccgc tcgaccagca ccttgctggt cagcgagacg ttgtcggccg
     1081 ccgaagcggc ggcgggagcg agaagcgcga agagcgcaag ccagatcttc atgggcgtca
     1141 tccttctcaa tcgatcttca cttggaacgt gatggttttg gtggcgccgg cggcgacggt
     1201 tccgagtccg acggtgacgg cggtgccggt gaagctcccc tggtcggcgt cggccgagtc
     1261 ggtgagcggg gcgccctcga gggtgaggga gccgggcttg taggtcgttc cggccggcac
     1321 ggggtcgctg acccggaggt tggcgaggtc gcccgtgccg ctgaccgttg ccgcgagcgt
     1381 gtaggtgatg gtcgagccag gcgcggcggc ggtgccgccg aaggcgtcgg cgacggtcgc
     1441 gctcttgagg aaggcgacgc tggccctggc tatccggtaa tagccgtcgt cgtccgaatc
     1501 ggcgccggtg gctccgacga cggcgttgcc gccgccctgg ccctggcccg cgaagctggt
     1561 tcccggagcg cccgatccgg tcttggcgac cgcggtgaga tcgacccggc cgcgctggcc
     1621 gtcgccggcg cccgcgggga tgctcgacag gacgaagacg gtgatcgact ggtccggatc
     1681 cagctgcgga tcgttgcttc cgctggcata gacggtgtcg accccggcgt cgtaggcgcc
     1741 gttggaattg ctatcgagaa cgatcgaggt gaccgaagga tcgaaatcgt cgccgccgcc
     1801 attggcgacg gtggcgagcg tgaagctctc cgcgccgttg ccgccgttgg tgattgtgaa
     1861 cttgagccgc tggccggtgc cgccggccga agccgtgacg tcggacggat cgctccaggc
     1921 cacgccgacg tcgagcagct cgtcgacctt cagggtgacg atattggaat ccacgctggc
     1981 ttcgtcgccg ttgggcagct cgtaggttgc ggtggcgacg ttggtgatgt cggtgccggc
     2041 cggggtcccc gccgcctgcg cggggctcgc gaaaacgcac gcggagaccg ccgtcagggc
     2101 ggccagggct gcatttttgc ggaacgccgg tgatttggcg ttcgtagcct gataacgcat
     2161 tggtgctccc cacgagcttt gatccgattt ggagctaatg gggggtgagg ggctaagaat
     2221 aagttaacgg cggattagct gttgagctta agccacgttc gaagggctgc ggtaaggaag
     2281 atgaacaagc tgcagggcaa ggaagtcatt gaaggagcga cgcaagtcga gccggtcgat
     2341 ccgaggtggg aaccggaggc ggtcgcctac cggcgcgacc ggctgctcgc cgcgttaacc
     2401 ctgatcgcgg gggctgggct ggccatcgcg ctccccttcg cgcttcgcgc cggagccgaa
     2461 ttcttcctac ctgtgacggc ggctctggtc atcgccgtcg ccctcgtgcc gatgctcgaa
     2521 tggctggaac ggcggcgggt gccttcgccc cttgccgcct tcacctgcat cctcgtcctc
     2581 atcaccatgg ccaacatcgc cgtcgcctcg atcgtcctcc cggcggcgga atgggtaagg
     2641 ctcctgcccg agcggatcgg ccgggtccgc gacactctgg cgccgctgat ggaggtctat
     2701 tcggacctcg agcgcttcat cgacgatatc gtcgagcagt tcggatcgca cgcggcggct
     2761 ccgcggacgg ttcagctcga gacccccaac tcgctcctcg acatcatcgc cacctcggcc
     2821 cccttcgcgg cgatccagat gttcttcgcc ctgctggtaa tcttcttctt cctcgcgggc
     2881 tggaccagca tgcgccggcg cacgatcacc gcccgctcca gcttcgacgg ggcgatgacc
     2941 accgcccgcg ccatccagga ggtggtcgac gccacctcca cctatctcgg cacgatcacc
     3001 atcgtgaaca tcgccatggg tctcaccgtg gccttcatcc tctacctgct cgacatgccg
     3061 acgccgctca tgtggggcgg gattgtcgcc gtcctgaact acatccccta tctgggcccg
     3121 atcgcctcgg tcctgcttct cgctctgggc gggctgatgg ccttcgccga cccctggtac
     3181 gccttccttc cggcgatctg cttcatcgga atccacctcg tcgaggcgaa cctcatcacc
     3241 cccttcttcg tcggccggcg cctgaccatc aatcccctgc tgatcctcgt cgccttgagc
     3301 ttctgggcgt gggtgtgggg gacgaccggg gccttgctgg cggtgccctt gctcatcatc
     3361 acgaagaccg tgctcgacgc cgccggaaag cccgacatcg ccggcttcct gttcgaggag
     3421 gggacgctga ccggcatcca ccacgaggaa catggcgagg ccgatcgcgc cgccgccgca
     3481 aagccttagc tccgcttgac agccacgggg ccagcgcata gttcgccccc tcccacgcgg
     3541 gtgtagctca gttggttaga gcgccggcct gtcacgccgg aggtcgcggg ttcgagcccc
     3601 gtcactcgcg ccatttccca tttctccccc gggcctgctg agtcataagc gccgccatgg
     3661 cgagccgcta cgaccgcatc ggtttgaact attcgaacct caggaagccc gatccccgga
     3721 tcgcggccag gatcgaggcg gccctgggag attgcaggac gatcctcaac gtcggcgcgg
     3781 gcaccggctc ctacgagccc atgggccggg aagtgaccgc cctggagccg tcgatcgaga
     3841 tgatccgcca gcgctccgcc ccggcggcgg ccgtcgtcca gggccgggcg gaagcgctgc
     3901 ccttcgcgga tcggagcttc gacgccgcga tggcggtcct gaccatccat cactggtccg
     3961 gccaggcgag gggcctcgcc gaaatgcgcc gggtgacgaa cggcccgatc gtcctcctca
     4021 cctacgatcc cgccttccgc ggcttctggc tcgccgatta catccccgga ctggtcgccc
     4081 tcgacgaggc gcagatgccc tcgatgaggg attatcagcg ctggctcggc ccggtcgcca
     4141 tctcgccggt gccgatcccc cacgactgca cagacggctt cctcagcgcc tattggcgcc
     4201 ggccggccgc ctatctcgat ccgcgggtca gggcggcaat gtcgtccttc tgggcgctcg
     4261 gcgacgtctc ggaggcgctg gcgaggctcg aggcggacct ggaaagcggc gcctgggcac
     4321 ggcgctacgc cgaactcctc gccctcgatg cctgcgactg cggctatcgt ctggtggtcg
     4381 cccgataaga agcggattgc ctcggccgcg agccttgcgc atgtgtaacg agtcgttcgc
     4441 caggagcatc atcgatcatg ccgcgccggc tcgccgcctt tctcctgctc gccgccttgc
     4501 ccggccccgc cccggctgcc ggcgaacgtg ccttcgaggc cgctctaagc gacttccgca
     4561 agctccaccg cgccgagact cggcgctcgg ccatcgccgg gagcagcttc tacatggtcc
     4621 gggacggccg aaccgtcgtg gccgaccacc tcggcgagca ggattcggag gctcgggtcc
     4681 cggtggacgc ccggacgatc taccattggg cgtcgatcac caagaccatg accggaatcg
     4741 cgatcctgca gctgcgcgac cgcggcctgc tgaagctgga cgatccgatc acccgcttcg
     4801 tccccgagct cgccgccgtg cacaacccgt tcggcgacac caatgcgatc acccttcgcc
     4861 agctgatgag ccacagcgcc ggcttccggg gcggcacatg gccgtggcgc gagcatgagt
     4921 ggcagccgtt cgagcccagg agctggtccc agctggcggc gatgcttccc tatacccgag
     4981 tcgagttccg gcccggcagc cggttcggct actccaatcc cgggatcgtc tatctcgggc
     5041 aggtgatcga gcgcctgtcg ggcgaggatt tcgaagtcta tgtcgacaag aacatcctca
     5101 agccgctggg catgcactcc agctatttcg accgcactcc gccccacctc ctgccgcacc
     5161 gctcgcgcag ttattacatc cgcgacggca agcgggccgc ggccccgttc gacgtggata
     5221 cgggagtgac ggtctcgaac ggcggcctca acgcgccgat gcccgacatg gcgagatatg
     5281 tcgccttcct gctcggcgac ccggcgcgag cgggcgaata cgatctcgtc ctgaagcgcg
     5341 cctcgctgga ggagatgtgg aggccgcaga tctccgccgg agaggatttc acccagggcc
     5401 ggatggcgaa gaccacccaa agcggcctgt ccttcttcat cgaccggggc aggggcgtcc
     5461 gcttcgtcgg ccacaacggc gaccagaacg gtttcagggc ctatctcagc ctctgcccgg
     5521 accagcgcct cggaaccctg ctggccttca acacggagac gagggaggtc cgcaacgacc
     5581 cctccaaccg cggaaccgcc gaatcgcgaa tcgcgctcgc caccgacagc ctgtgcgagg
     5641 cgctggcgaa gtcggggccg gcataattct cgccggttga gccctcggcc tggcccagcg
     5701 ccgcttcata aacgaacttc gcatcttcga ccgtctgcct tgtgccgctt tccggcagcg
     5761 cctactttcc cctcgcccac aggagccgcg gattatgacc ggacgcgcgc aagagaaaga
     5821 cgaccaggcg gaagccgagc cgctcgatcc gcccttcgcc gagcgctgcc gggagtctct
     5881 cgccgatccg cggcccgaca ttcccgccgc ggaggtgcga gcctatctgg aagcgctgga
     5941 cgagagccgg ctgaagcgcg gtgcgtgagc tggtctaccg tccgttggct cgggaagatc
     6001 tggctgaaat ctattggaga cccgcggagg cggaggggtt cgcctccgcc tccgagctgg
     6061 tcgctgcact gcaggatcgc tgcgaaggcg tgaccacctt ccctgaacga ggtacgcccc
     6121 gtcccgaaat cggtcccggc gttcgatcca tccccttcag gcgcaaggcc gtgatcgcct
     6181 acttggtccg tccggatgca gttgtgatta tcggaatcat gtacggcggc cgcgacctcg
     6241 ccgctctgaa ggagccgggg cggctcggcc cgcgatccac ccgtccacct cacgttccag
     6301 gatcgagagc gggagcgatc ccgagccgat caggagatcg tggaatgccc tgatatcgaa
     6361 cctggggccg agctcgcgtt cggccttgcg gcggagctcc atgatcttga gcatgccgat
     6421 cttgtagccg gtcgcctggc ccggcagggt gatgtagcgg cgaacctcgg agcgggcctg
     6481 gtcgggcgag gcgcggccgg tcgcgaccat atagtcgacg gcccggtcct cggtccaacc
     6541 cttggcgtgg atgccggtgt cgacgaccag ccgcgcggcc cggaacagct ccgcttccag
     6601 ccgcatgaag tcgctggcga catcgggata ggcgaccatc tccttgcaca gcgcctcggc
     6661 gtagagggcc cagccttcgc cgaaggcgac gtagcgcgcc gactggcgga atttgggccc
     6721 ggtcttctgg cggacctgga tgtcgccctg catgacgtgg ccgggcacgc cctcatggca
     6781 catcaggtcg gggatggcgt cgacggccgg atcgctgttg accccgagca ggtggacata
     6841 gacccgcccc ggccgagttc cgtccgggct gggtgccgcg gcatgggcgg cgccgccggc
     6901 gacctcgctg aacgacggct cgcgcaccac ctccatccgg tgggcgggca gctggttgaa
     6961 atatttcggc aggagcgtgc gggtgcgcgc gatcgcttca ttggccttgc gcagatattc
     7021 ggagcggagc gcgtcggtcc agggccgcgg cggattgcgg cgctcgcggt cggcataata
     7081 agccgcgcgg tccttgaagc cggcctgcct tgcaagcgcg tcctgctcgc cctcgattcg
     7141 ctttacctcg ctcaggccga tccggtggat ctgctccgca ttgaggtcgg tggtggtgtt
     7201 gagcttgagc gcggcggcat aatagtccgc cccaccgggg agcgagatcg cgcccacccg
     7261 gccgctcgga gcggcggcca gggcgctttg tccccaggcg acgacccgct cataggccgg
     7321 cctgatcgac agcaaggccg ttcgggtctc ctcgagcagc gcctcggcct cggcgggcgt
     7381 gaccttgccg ccggtccgga gcttggcgac cttggcctgt gcgtccgccc acaagggtga
     7441 cggcggcccc gcatcgaacg gagcgccggt gacgatcgct cggcttccgg cgatcacccg
     7501 ctcgacctgg aatctcggtg cacgcacgcc gatcgcatcc gatgcccggc tttggacgat
     7561 ggcctcgtcc agcaccgccg gaatcgcgcg cagccgtgcc gcataggcgc gcatgtcgcc
     7621 cgcatcctgc acattatggg tgttgatgag gaagtccggc agccgcgaat gggccgaata
     7681 gaggaaggaa tagagagcgg gggaatattg gcgatagcgg tagctaagct cggcccggtc
     7741 cagctcgagc gcccagatat cgtaattggt ccgggcgctc gccgacagct tggaccggtc
     7801 gaattgcgac ttcatccgcg caacgctggc gcggcgccat tccagccggc gaagcgcggc
     7861 cgcctcgtcg atgtcgtcga gccggtcctg cccctccttc aagcccaatt gggtggcgag
     7921 ctgcggccgc tgccgcagct cctcggcgaa ttcctggtcg aggaaagcat agagccgggt
     7981 atcctcgctg gccgccgtag ccgccggcgc ctgcgcctgc acggcggcgg tgacaggcac
     8041 ggcgcccgca agagccgctg cggcggtcag ccaatatgtt cttcgcatgg tcatcctctt
     8101 cgatccggcg agcttcctaa tcgccttggc aagcccttga aagccctatg cttccggcaa
     8161 cggttcggcc cttaccccgg cggctgaagt atatactgtc cccgttttcg cgccttcctc
     8221 tttgcggctt cgcggcgcgg ttgccggctt catcctgaac ttgtttccgc atccagggac
     8281 gaagcgaccc gcggtcccgg taccggaccc tgcaaaaggt tcagggtgac cgcgccggag
     8341 gttcgttcca tggcgcctcg cgcgtgtaga cctgcgaaag gtcgtcctcc caggcgccgc
     8401 cttttttcga catccggcct ttgctcacca gcctgtcgcc atcggcggcg atggtcagcg
     8461 attgggcgaa ttccgcatcg tcgtggcgcc acgtcacgct ccggtcggcg gcggtcacgt
     8521 cgaggatgcg ggagacttcg cgctcgtcga aatagatcat ggtgaggtcg ccgcaggcgc
     8581 cgtcgctgcc gatgatcgcc actccgtccg gaaattgcgg gtggtcgacc tgcgagcgca
     8641 tgatcaggaa cgcgccgccc tcgtgcgggg cgaagacggt ccggccgcac aaggtctcgc
     8701 ccggcacgag gggatgggtt ccggtggtcc tccactcgcc gatcagaaat tcgagcggct
     8761 tcagcgccgg attgggaatg agcgccccgg cgcccggccc ggaagatcgg ccccttctca
     8821 cttccgccac cgtcccaatt ccatccacct gagcagcggc ttgagcggca ggatccagac
     8881 gatgccggcg gcgatgaaga acagcgcctt caacggccag ggcagctccg gcagcgcttc
     8941 cgcgacggtc acgaccacga acccccagac cgcgatcagg agcaggagca ggagcattcc
     9001 cgcgggcttt cgccagcttg gcctgggggc gttcaccggc agtcgatcca ctcgcgcggc
     9061 gtggcgatgg cgtcgagggg gacgtcccac gggtcggcgg ggatcgggtc ggcgaggatc
     9121 tggagctccc aggcgaggcc gatggtgaag atctggccgc cttcccgaag gtgcgagagg
     9181 gcccggtc
//
LOCUS       DASWNU010000272         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_218215, whole genome shotgun sequence.
ACCESSION   DASWNU010000272 DASWNU010000000
VERSION     DASWNU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218215"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VGB57_05365"
     CDS             <1..337
                     /locus_tag="VGB57_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF969 family protein"
                     /protein_id="HEY0130814.1"
                     /translation="ATDNVGLFFGEDIFFAIGSIVLIQQTLEAYGIPLSPLELAVWAI
                     PTAIAAFIVHGTRLMMLDRKLASLQRSSGEGDRPKGGGGVADEASANDPSTPLRGVPL
                     PISDGEAKS"
     gene            334..1284
                     /locus_tag="VGB57_05370"
     CDS             334..1284
                     /locus_tag="VGB57_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEY0130815.1"
                     /translation="MITGQHVLYLVGAMFAAFALLSAFDRTNPKRLGNAAFWGLFALS
                     LIAGSWLGDLANGCIVLALAALAGLKLIGRGASETGEAEREAKGERFGNALFLPILIV
                     LVIAVTGTLLYLYTPLYETKLFEKNRETFVLLGAGVLIALAAIFAWLKPPPLAPLQEG
                     RRLIDAVGWAAVLPMMLAALGVVLADTGVGRTIGGLILTVIPEGTIFPAVIAFGLGMA
                     LFTILTGNAFAAFPVMATAIGVPLLIQNYGGDPAAIGAIGMLAGFCGTLMTPMAANFN
                     LVPAALLELKDQNGVIKAQIGTALPLLAINILLIYFLAFR"
     gene            1395..2387
                     /locus_tag="VGB57_05375"
     CDS             1395..2387
                     /locus_tag="VGB57_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEY0130816.1"
                     /translation="MRHALTPETAARFAGIALGHVTREFPHKLDHVMDGPEDALSPRE
                     LHPIFFGSFDWHSCVHGWWLMLRLRRLFPDLPVARRIEDMADEMLAPARVAGELAYLD
                     RAYSGGFERPYGWAWLLALHAEAQSHSDRPWAADLEPLARAFGERFQLYLPKLTSPIR
                     VGTHFNTAFATILALEWAEANDPPLAGLIRERARTWYGQDRDCQAWEPGGDEFLSSAL
                     TEALCMRRTQGDEKFRSWFGRFLPDLAGGEPRSLFTPATVSDRSDGKIAHLDGLNLSR
                     AWCWRQLAPALEPELAELARRSADEHLDASLPHVAGDYMGEHWLATFALLALEA"
BASE COUNT          392 a          911 c          831 g          468 t
ORIGIN      
        1 cgccaccgac aatgtcgggc tcttcttcgg ggaggacatc ttcttcgcga tcggatcgat
       61 cgtcctcatc cagcagactc tcgaggccta tggaatcccg ctgtcgccgc tggagctggc
      121 ggtgtgggcg atcccgacgg cgatcgcggc cttcatcgtc cacggcaccc gactgatgat
      181 gctggatcgc aaacttgcct ccctgcagcg cagcagcggg gagggggacc gcccgaaggg
      241 tggtggaggg gtagctgacg aggcctctgc caacgacccc tccaccccgc tacgcggggt
      301 ccccctcccc atctccgatg gggaggcaaa atcatgatca ccggccagca tgtcctctac
      361 ctggtcggcg cgatgttcgc cgccttcgct ttgctcagcg cgttcgaccg gaccaatccg
      421 aagcggctcg gaaatgccgc cttctggggc cttttcgccc tgagcctgat cgccggctcc
      481 tggctcggcg acctggcgaa cggctgcatc gtcctcgcgc tcgccgcgct cgccggcctg
      541 aagctgatcg gccggggcgc ttcggagacg ggcgaggccg agcgcgaggc caaaggcgag
      601 cgcttcggca acgctttgtt cctcccgatc ctgatcgtcc tcgtcatcgc cgtgaccgga
      661 accctgctct acctctacac gccgctctac gagacgaagc tgttcgagaa gaatcgcgag
      721 accttcgtcc tgctcggcgc cggggtgctg atcgctttgg ccgcgatctt cgcctggctg
      781 aagccgccgc cgctggcgcc gctccaggag gggaggcggc tgatcgacgc ggtcggctgg
      841 gcggcggtgc ttccgatgat gctcgccgcc ctgggcgtcg tcctcgccga caccggggtc
      901 ggccggacga tcggcggcct catcctgacc gtgatcccgg aaggcacgat ctttccggcg
      961 gtgatcgcct tcggcctcgg gatggcgctc ttcaccatcc tcaccggcaa cgccttcgcg
     1021 gccttcccgg tgatggcgac ggcgatcggg gtgccgcttc tgatccagaa ttacggcggc
     1081 gatccggcgg cgataggcgc gatcgggatg ctcgccgggt tctgcgggac cttgatgact
     1141 cccatggccg cgaacttcaa tctggtgccg gccgccttgc tcgagctcaa ggaccagaac
     1201 ggagtgatca aggcgcagat cggaacggcg ctgcctttgc tcgccatcaa catccttctg
     1261 atctatttcc tggcgttccg gtagccgtcg cccccggcga gccgtcatgc tgaacttgtt
     1321 tcagcatcca gtttcgagca gcggtgcggg cggagaactg aaccctgaat cgggttcagg
     1381 gtgacggaga aaagatgcgc cacgccctaa cccccgaaac cgccgcgcgc ttcgccggga
     1441 tagcgctcgg ccatgtgacc cgcgaattcc cccacaagct cgaccatgtc atggacgggc
     1501 cggaggacgc tctatcccct cgcgagctcc atccgatctt cttcggaagc ttcgactggc
     1561 acagctgcgt ccacggctgg tggctgatgc tccgccttcg gcggctgttc ccggacctgc
     1621 ccgtcgcccg ccggatcgag gatatggccg acgagatgct cgctccggcc agggtggcgg
     1681 gcgagctcgc ttatctcgac cgcgcttatt ccggcggctt cgagcggcct tatggctggg
     1741 cctggctgct cgccctccac gccgaggccc agagtcattc cgaccggccc tgggcggcgg
     1801 acctggagcc gctcgcccgg gccttcggcg agcgcttcca gctctatctg ccgaagctta
     1861 cgtctccgat ccgggtcggc acccatttca acaccgcctt cgcgacgatc ctcgcgctgg
     1921 aatgggccga ggcgaacgat ccgccgctcg ccggcctgat ccgagagcgg gctcggacct
     1981 ggtacggcca ggatcgcgac tgccaggcgt gggagccggg gggcgacgaa ttcctttcct
     2041 cggctctcac cgaggccctg tgcatgcggc gcacgcaggg cgacgagaaa ttccggagct
     2101 ggttcggccg tttccttccc gacctcgccg gcggcgagcc gcgcagcctg ttcacccccg
     2161 ccaccgtctc cgaccgatcg gacggcaaga tcgcccatct cgacggcctc aacctcagcc
     2221 gcgcctggtg ctggcggcag cttgctcccg ccctggagcc ggagctggcg gagctcgccc
     2281 ggcgctccgc cgacgagcat ctcgatgcca gccttcccca tgtcgccggc gactatatgg
     2341 gcgagcattg gctcgccacc ttcgcgctgc tcgcgctgga ggcatgacgc cctttcgtgc
     2401 tatcgtcgcg ccatgggaga tggggcatgg gaaagcagag gaaactgacc gccggcgaaa
     2461 tcgcgctcgc ccgcaccgcc ttcggtgaca agatccaata tgacagagtg aggctgagcg
     2521 acggaccggg caccaacccg atggcccaca tggcgttcgc caagggcaat cccgcgatca
     2581 cgatcggcaa tacggtttat tt
//
LOCUS       DASWNU010000273         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_478933, whole genome shotgun sequence.
ACCESSION   DASWNU010000273 DASWNU010000000
VERSION     DASWNU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478933"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGB57_05380"
     CDS             <1..300
                     /locus_tag="VGB57_05380"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEY0130817.1"
                     /translation="AAVHVLLLRLLAMVETVRTGDPFVPENAIRLRTIAWCLLVVQLF
                     ELACGIFIGVLERVGADVGEWDPSLSGWLAVLLVFVLARVFEEGARIRAELEAVV"
     gene            302..562
                     /locus_tag="VGB57_05385"
     CDS             302..562
                     /locus_tag="VGB57_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0130818.1"
                     /translation="MAIIVKLDDMLYARRMTLTELSARVGITLANLSILKTGKAKGMR
                     FATLEAICEALDCQPGDLLEHKRAEDGDEDSPAAAASWGGGS"
     gene            559..1248
                     /locus_tag="VGB57_05390"
     CDS             559..1248
                     /locus_tag="VGB57_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130819.1"
                     /translation="MNALTWLHIAGGSAAIVAGALAVAARKGGRVHAGAGTVFAVSML
                     VLGATASVLAQPKGDIGLGLGGLLTCYFVATSWVTARRRDGTTGRFEIAACALVLIAA
                     AAIFWSALAGTMTTPVGTGPIFAFAGVCLIAGLLDLNVILRGRLTPARRLSRHLWRMC
                     FAFFIATGSFFLGQQDVMPEAVRGSPLLFVLACAPIAVMLFWLVRLRFAKTIGRLTLR
                     TPKARPGLAPQ"
     gene            1245..1664
                     /locus_tag="VGB57_05395"
     CDS             1245..1664
                     /locus_tag="VGB57_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130820.1"
                     /translation="MTGVSPIRLWVMRAMYLVMALGIGATVWPSILSHGSDLPRMTGV
                     AFALLGTIGLLALLGVRYPLQMLPVLLVELVWKAIWLAAFALPRWLDGSLDEGMRSSI
                     LDTSLGAILLLVIPWRYVWANYVIKAGDPVSKRRSSA"
     gene            1674..1934
                     /locus_tag="VGB57_05400"
     CDS             1674..1934
                     /locus_tag="VGB57_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130821.1"
                     /translation="MDLDDQLRRYFGTADFAAIPPAALEAGIERMKVDLGLERDRGNR
                     FALWALLHMLGAAPDLDVAFPDERDREAARNFMDLAGRAEGT"
     gene            2041..>2653
                     /locus_tag="VGB57_05405"
     CDS             2041..>2653
                     /locus_tag="VGB57_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0130822.1"
                     /translation="MDDIATKAATGVSGLDDILAGGLRRGRVYLLEGSPGTGKTTIAT
                     QFLLEGAARGERGLYITLSETETELRESALSHGWEFDEPNEIFELVPPESLLDDDQQQ
                     SLLYSSDLELGETTKRIFEVVERVQPKRIVLDSLSEIRLLAQSSLRYRRQILALKHYF
                     SHHDSTVVMLDDLTTEVNDKTVHSVAHGVVRLEELAPEYGAERR"
BASE COUNT          378 a          894 c          896 g          485 t
ORIGIN      
        1 gcggcggtcc atgtgcttct gttgcggctg ctcgcaatgg tcgagacggt tcggaccggc
       61 gatcccttcg ttccggagaa tgcgattcgg ctcaggacga tcgcctggtg cctgcttgtg
      121 gtccagctgt tcgagcttgc ctgcggaatc ttcatcgggg ttctggagcg ggtgggcgcc
      181 gacgtgggcg aatgggaccc gtcgctcagc ggatggctgg cggtgctcct ggtgttcgtg
      241 ctggcgcgcg tgttcgaaga gggcgcccgg atccgagccg agctggaagc ggtggtctga
      301 catggcgatc atcgtgaagc tggacgacat gctctacgcc cgccggatga ccctgaccga
      361 gctgtcggcg agggtcggca tcaccctcgc caacctgtcg atcctgaaga ccggcaaggc
      421 caagggcatg cgcttcgcga ctctcgaggc gatctgcgag gcgctcgact gccagccggg
      481 ggacctgctc gagcacaagc gagcggaaga cggggacgaa gactcgcccg ccgccgcggc
      541 aagctgggga gggggctcat gaacgcgctt acctggctgc acatcgccgg cggctccgcc
      601 gccatcgtcg ccggcgccct cgcggtcgcg gcgcgcaagg ggggtcgggt gcacgccggc
      661 gccgggaccg tgttcgccgt ctcgatgctg gtcctgggtg ctacggcttc ggttctggcc
      721 cagcccaagg gcgacatcgg cctcggcctc gggggcctcc tcacctgcta tttcgtcgcc
      781 acctcctggg tcacggcccg ccgccgcgac gggacgaccg gcaggttcga gatcgcggcc
      841 tgcgcgctcg tgctcattgc cgccgcggcg atcttctggt ccgccctggc ggggaccatg
      901 accacgcctg tcggcaccgg cccgatattc gctttcgccg gagtgtgcct gatcgccggc
      961 ctgctcgacc tcaacgtcat cctgcgcggc aggctgacgc ccgcccggcg gctttcccgc
     1021 cacctctggc ggatgtgctt cgccttcttc atcgccacgg gctccttctt cctcggccag
     1081 caggatgtga tgcccgaagc ggtgcgcgga tctccgctcc tgttcgtcct cgcctgtgcg
     1141 ccgatcgcgg tcatgctgtt ctggctggtc cggctccgct tcgcgaagac gatcggccgg
     1201 ttgaccctgc gcacgcccaa ggcgcggccg ggcctggcgc cgcaatgacc ggggtttcgc
     1261 cgatccggct gtgggtgatg cgggccatgt acctggtcat ggccctgggc atcggggcga
     1321 cggtctggcc gtcgatcctg agccacggct ccgatctgcc gcggatgacc ggagtcgcct
     1381 tcgctttgct cgggacgatc ggcctgctgg ccctgctggg ggttcgctat ccgctgcaga
     1441 tgctccctgt gcttctggtc gagctggtct ggaaggcgat ctggctcgcc gccttcgctt
     1501 tgccgcgctg gctggacggg agtctcgacg aagggatgcg gagcagcatc ctcgacacgt
     1561 cgctgggcgc catcctcctg ctggtgatcc cgtggcgcta cgtctgggcg aattacgtga
     1621 tcaaagccgg cgacccggtt tctaagcggc gttcgtccgc ctagggacgc aggatggatc
     1681 tcgacgacca gcttcgccgc tatttcggaa ccgccgattt cgccgccatc cccccggccg
     1741 ccctcgaagc cgggatcgag cggatgaagg tcgatctcgg cctggaaagg gaccggggca
     1801 accgcttcgc cctctgggcg ctcctccaca tgctcggcgc cgccccggac ctggacgtcg
     1861 ccttcccaga cgagcgcgac cgcgaagcgg ctcgaaattt catggacctc gccggccggg
     1921 cggagggaac ctgactcgcg gtttcgcggt tttccgcttc gggccgcgaa ggttttacct
     1981 ccgaaacggc cgatacaggt taggtctccc gcgcttccca gtcgcggagc gagggaattt
     2041 gtggacgaca tagcgaccaa agccgcgacg ggcgtttccg gactggacga catcctggcc
     2101 ggcggcctca ggcgcggccg cgtctacctg ctcgaaggca gccccggcac aggcaagacc
     2161 acgatcgcca cccagttcct gctcgaaggc gcggcgcggg gtgagcgcgg gctctacatc
     2221 actttgtcgg agacggagac cgagcttcgc gagagcgctt tgtcccacgg ctgggaattc
     2281 gacgagccga acgagatttt cgagcttgtg ccgccggaaa gcctgctcga cgacgatcag
     2341 cagcagagcc tgctttattc gtccgacctc gaactgggcg agaccaccaa gcggatcttc
     2401 gaggtggtcg agcgggtgca gcccaagcgg atcgtcctcg acagcctgtc ggaaatacgg
     2461 ctgctcgcgc aaagctcgct tcgctaccgg cgccagatcc tcgccctcaa gcattatttc
     2521 tcgcatcacg attccaccgt ggtgatgctc gacgacctca ccaccgaggt gaacgacaag
     2581 acggtccaca gcgtcgccca cggggtcgtt cggctcgagg agctcgcgcc cgaatatggc
     2641 gccgagcggc ggc
//
LOCUS       DASWNU010000274         5331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_36157, whole genome shotgun sequence.
ACCESSION   DASWNU010000274 DASWNU010000000
VERSION     DASWNU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36157"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGB57_05410"
     CDS             complement(<1..236)
                     /locus_tag="VGB57_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0130823.1"
                     /translation="MTLSYPARLVPTDDGLVIARFPDVPEASAVGKTEEEALAGAVDV
                     LDAVLGGYVLDGRPIPAPSDICGAPQVETEKFSLL"
     gene            complement(233..406)
                     /locus_tag="VGB57_05415"
     CDS             complement(233..406)
                     /locus_tag="VGB57_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130824.1"
                     /translation="MRPREIFTLARTYREMAGRCREPAARVLLDMCARGYEEEAARFG
                     SRLIPASQAERAP"
     gene            552..1208
                     /locus_tag="VGB57_05420"
     CDS             552..1208
                     /locus_tag="VGB57_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0130825.1"
                     /translation="MTTAITITAFETSPDRGAGLARDMPVRWALEEAGQPYRVRLLSF
                     AAMKQPAHHRLQPFGQIPTYEEGDLVLFESGAIVLHIARRAGLLPDDANARARAVAWM
                     FAALSTVEPPVVEREAFILTERDKPWHEERLPLLETRIRARLDDLSARLGDGDWLDGG
                     FSAGDLLMVSVLRRLGGSGILDDYPNLSAYIARGEARPAFKRAFAAQLAVFTGGAPTD
                     "
     gene            1515..3182
                     /locus_tag="VGB57_05425"
     CDS             1515..3182
                     /locus_tag="VGB57_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY0130826.1"
                     /translation="MLKSGLVIALLAVGQTAPAQQPSPGAGGQLQQIPPAPLPEKAAP
                     DMRVENMQSAPAPAAEGATLRVDKLLVTGNTLFGEAELVAAAAVVPGNDLSLAQLRDA
                     AARIAAFYNERGYFLAQAYLPAQDIKDGAVTVAVVEGRYGTTGFSNRAGLSDGAVSRA
                     LAGLKTGDPVTAAPLERRLLLLSDIPGIKVRSTLAPGTDVGTSNLMINITQGGRITGS
                     VEADNGGNRYTGAYRLGGSVNLNNPTGIGDVLSLRLLGSTGKLGYGRAAYQATVGSAT
                     VGVAVTHLRYELGREFRSLEADGTANIASVFGSYPMLRSRDANLYALAGIDAKWLTDR
                     IDLVSTRSRRTSRALNLGFSGDTRDGLGGGGRNLFSAGLAVGDLHLQSPLDRAADALT
                     ARSEGGFSRLQLSAARLQAVSGPLSLYAAVRGQFAFGNLDSSEKMELGGAYGVRAYPE
                     GEAYGDEGWLGTLEARLALSRWTGELPGELQLFGFVDLGEVKFADEPWSAGSNRGHRS
                     GAGAGLGWAAPGGVVAKVSYARKLGDAVATSGPDRDGRFWFQISKLF"
     gene            3233..>5331
                     /locus_tag="VGB57_05430"
     CDS             3233..>5331
                     /locus_tag="VGB57_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEY0130827.1"
                     /translation="MNNIQHPTLRYKHRGAAVIALPRSGALFGSTAMVRLAGLALLGW
                     AASFPFGSAASAQTVPTGGTVSAGSASISTGPNNVTVTQSSQNAAINWQTFSIGQESS
                     VVFVQPNSASVALNRVLGADPSVILGSLTANGKVFLVNPNGILFGKGANVNVGGLVAS
                     TLGLADADFMAGRYKFSGSGGAVLNQGSITANSGYVALLGANVGNDGTIQANLGTVAL
                     AAGEAITLDVAGDGLLNVAIDKGAVNALVRNGGLLRADGGQVLMTAQAAGQLLRTVVN
                     NTGAIEARTLENRSGVIKLLGDMESGTMSVAGVLDASAPNGGDGGFIETSAAIVTIAD
                     GVRVTTAASQGQTGTWLIDPQDFTIAATGGNISGATLSAQLVTNSVVISTIVGPGAAL
                     PGEGHIHVNDAVAWTSSGPSGTATTLTLNAAGDVNINAAITATKGNLVVCCGGDVNVK
                     APITTTNGSVLLNAGQNVNVFHAITTTDGNIALCAGHDVHIDAKITLTRGSTIVAQSL
                     GLPVGLTLIAGADGTGPGEGGGTVVFAPLAPPITVTEAPVSIAYNPVSYAAPTDFSTK
                     FVLTEGAALTQRMLLFPHAEKVFDGTTAAVLSGFNTATSGVPTGVTLVAGPGATAAFD
                     SPAPGSNVGITFSGYTLAGANAGQYTLAGFCCVSTFRTSGAITAAAAPPPPAPPPPAP
                     PPPAPPPPAPPPPAPPPP"
BASE COUNT          873 a         1906 c         1687 g          865 t
ORIGIN      
        1 aggaggctga acttctcggt ctccacctgc ggcgcgccgc aaatgtcgct cggcgccggg
       61 atcggccttc cgtcgagcac atagccgccg agcaccgcgt cgagcacgtc cacggccccc
      121 gccagggcct cctcttccgt cttcccaacc gcgctcgcct cgggtacgtc ggggaagcgg
      181 gcgatgacca ggccgtcgtc ggtggggacg agccgcgcgg gatagctgag cgtcacggcg
      241 cgcgctccgc ctgggaggcc gggatcaggc gggagccgaa gcgcgccgcc tcctcctcat
      301 agccgcgcgc gcacatgtcg agcaggaccc gagccgccgg ctcgcggcag cggcccgcca
      361 tctcgcgata agtccgggcg agcgtgaaaa tctctcgtgg tcgcatgatt gatccaggtt
      421 agcctcaggc aaaaccgatt tagaatcagc ttgttccccc gaagcttcgc ccaagagcga
      481 aaacgcccgc ggcagattgt tgcccaaggc cgcctcgccg ggcatcctgc agatccgacc
      541 ggagctttgc gatgacgacg gccatcacca tcaccgcctt cgaaacctcc cccgaccgcg
      601 gcgccgggct ggcgcgcgac atgcccgtcc gctgggctct ggaggaagcg gggcagccct
      661 atcgcgtccg tcttctctca ttcgccgcga tgaagcagcc cgcgcaccac aggctccagc
      721 cgttcgggca gatcccgacc tatgaggaag gcgatctcgt cctgttcgag tccggcgcga
      781 tcgtgctcca catcgcgcgg cgtgccggcc tgctgccgga cgatgcgaac gcccgcgccc
      841 gcgccgtcgc ctggatgttc gccgcactca gcacggtcga gcccccggtc gtcgagcgcg
      901 aagccttcat cctgacggag cgcgacaagc cttggcacga ggagcgcctg cccctactcg
      961 agacccgcat ccgcgcccgc ctcgacgacc tttccgcccg cctcggcgac ggcgactggc
     1021 tcgacggcgg gttcagcgcc ggcgacctgc tgatggtgtc ggtgctgcgc agactgggcg
     1081 gatccggcat cctcgacgac tatccgaacc tctccgccta catcgcccgc ggcgaggcgc
     1141 ggcccgcctt caagcgtgcg ttcgccgctc aattggcggt tttcaccggc ggagcgccga
     1201 cggattgaca gagattttgt tgctcctgaa aatagtcatt agaaatcggt gttgttagag
     1261 aggtccttct aggaacacgg atggatcgcg ccgaggtttc tgactgagaa ttattgaaat
     1321 aattcacagt cttaaccata tgttaatcta gtattattat gcatacctcc tagcgctgtt
     1381 ttgccagaag ctcacgagtt gatggtctgt ggcgctgaac gtttggagcg aattcggcca
     1441 tcgacctcgc ccggtacgtc tctcggctgc tcgggggcgt cacgccctgt cccgcagcgg
     1501 cccgcgaggt gatcatgttg aagtccggcc tggtaatcgc attgctggcg gtcggccaaa
     1561 ccgcccctgc gcagcagccc tcccccgggg caggcgggca gcttcagcag attcctcctg
     1621 cgccgctccc cgagaaagcc gcgcccgata tgcgggtcga gaacatgcaa agcgcgcccg
     1681 cgcccgccgc cgaaggtgcg acgctccggg tggacaagct gctcgtcact ggaaacacct
     1741 tgttcggcga ggccgagctg gtcgccgccg cggccgtcgt gccgggcaac gatctgtccc
     1801 tcgcccagct gcgcgacgcc gccgccagga ttgccgcctt ctacaatgaa cgcggctatt
     1861 ttctggcgca ggcctatctt cccgcgcagg acatcaaaga cggcgccgtc actgtcgccg
     1921 tagtcgaggg ccggtacggc acgaccggct tcagcaatcg ggccggattg tccgacgggg
     1981 ccgtgtcgcg tgccttggcc ggcttgaaga ccggcgatcc ggtgaccgcc gcaccgctcg
     2041 agcgccgcct cctgctgctt tccgatattc ccggcatcaa ggtaagatcg accctcgctc
     2101 ccgggacgga cgtcggcacg tcgaatctca tgatcaatat cacccaaggc gggcgcatta
     2161 ccggcagcgt ggaggccgac aatggcggca accgctatac cggcgcctac cgcctcggcg
     2221 gctcggtcaa cctcaacaat cccaccggta tcggcgacgt tctcagcctg cgcctgctcg
     2281 gctcgaccgg aaagctgggc tacggccggg ccgcctatca ggcgaccgtc ggatccgcga
     2341 ccgtcggcgt ggcggttacc catttgcgct acgagctcgg ccgcgagttc aggagcctcg
     2401 aggcagacgg gacggcgaac atcgccagcg tcttcggcag ctatcccatg ctccgttccc
     2461 gggatgcgaa cctttacgcg ctcgcgggca tcgatgcgaa atggctgacg gaccggatcg
     2521 acttggtgtc gacgcgatcc cgcaggacga gccgagcgct gaacctgggc ttcagcggcg
     2581 atacgcgcga cggcctgggg ggcggcgggc ggaacctgtt ctcggcgggt ctcgcggtcg
     2641 gcgatctcca cctccaaagc ccgctcgacc gggctgcgga tgcgctgacc gcgcgcagcg
     2701 agggggggtt cagccggctc cagctcagcg ctgcccgcct ccaggcggtc agcgggccgc
     2761 tttccctcta tgcggcggtg cgcgggcagt tcgctttcgg caatctcgat agctcggaga
     2821 agatggagct tggcggcgcc tatggcgtgc gcgcctatcc cgagggcgaa gcgtatggcg
     2881 acgaaggctg gctcgggacc cttgaagccc gcctggcact gagccgctgg acgggggagc
     2941 tgccgggcga gctccagctg ttcggcttcg tcgacctggg cgaggtgaag ttcgccgacg
     3001 agccgtggtc cgccggatcg aaccggggcc atcgcagcgg ggccggcgcc ggtttgggct
     3061 gggctgcgcc gggcggcgtc gtcgccaaag tctcctacgc ccgcaagctg ggcgacgcag
     3121 tagcgacctc cggccccgac cgggatgggc gcttctggtt ccagatctcc aagctgttct
     3181 gatggcacga ccagcaaaag cgcggcccac gcctctctcg aaggacgtca tcatgaacaa
     3241 catccaacat ccgaccctcc gttacaaaca ccggggcgcc gccgtcatcg ctttgcctcg
     3301 cagcggcgcc ctgttcgggt cgactgccat ggtacggctc gccggcctgg cgctgctcgg
     3361 ctgggcggcc tccttcccct tcggatcggc ggcctctgcg cagaccgtgc ccaccggagg
     3421 aaccgtctcg gccggcagcg cgagcatctc caccggcccg aataacgtca ccgtcaccca
     3481 gtccagccag aacgccgcta taaactggca gaccttctcg atcggccagg agagcagcgt
     3541 cgtcttcgtt cagccgaaca gcgcctcggt cgccctcaac cgggtgcttg gcgcagaccc
     3601 ctccgtcatc ctcggaagcc tcaccgccaa cggcaaggtc ttcctggtca acccgaacgg
     3661 aatattgttc ggcaaaggcg ccaatgtgaa cgtcggcggc ctcgtcgcat cgaccctggg
     3721 cctcgccgac gccgatttca tggcaggccg ctacaaattc tccggcagcg gcggcgccgt
     3781 gctcaaccag ggctcaatca ctgcgaacag cggctatgtc gcgttgctgg gagcgaatgt
     3841 cggcaacgac ggtacgatcc aggccaatct cggaaccgtc gcgctcgcgg cgggcgaggc
     3901 catcaccctc gacgtcgctg gcgacgggct gctcaacgtg gccatcgaca aaggcgcggt
     3961 gaacgccctc gtccggaacg gcggcctgct gcgcgccgac ggcggacagg tgctgatgac
     4021 ggcccaggcc gccggccagc ttctgagaac ggtcgtcaac aacaccggcg ccatcgaagc
     4081 gcggacgctc gagaaccgca gcggcgtgat caagctgctc ggcgacatgg agagcggcac
     4141 gatgagcgtc gccggcgtgc tggacgccag cgccccgaac ggcggcgacg gcggcttcat
     4201 cgaaacttcc gccgcaattg ttaccattgc ggacggggtc agggtcacca ccgccgcgtc
     4261 gcagggccag accggcacct ggctgatcga tcctcaggat ttcacgatcg ccgccaccgg
     4321 gggcaacatt tcaggagcca cgctttcggc ccaactcgtc acgaacagcg tggtcataag
     4381 cacgatcgtc gggccgggcg ccgctcttcc gggcgagggc catatccatg tcaacgatgc
     4441 ggtcgcctgg acctcttcgg gaccctccgg gaccgcgacg accctgactc tgaacgccgc
     4501 cggcgacgtc aacatcaatg ccgcgatcac cgccaccaag ggaaatcttg tcgtgtgttg
     4561 tggcggcgac gtgaacgtca aggcgccgat cacgaccacc aacggcagcg tcctgctcaa
     4621 tgccgggcag aacgtgaacg tcttccacgc gatcaccacc acggacggca acatcgccct
     4681 gtgcgccggc cacgacgtcc acatcgacgc gaagatcacg ttgaccaggg gcagcacgat
     4741 cgtcgctcag agcctgggcc tgccggtcgg cctgaccctg atcgccggcg ccgacggcac
     4801 cggcccgggc gagggcggcg ggacggtcgt cttcgccccg ctcgcaccgc ccatcacggt
     4861 caccgaagcg cccgtctcga tcgcctacaa tccggtctcc tatgcagcgc cgacggactt
     4921 ctcaaccaag ttcgttctca ccgaaggggc ggctctgacc cagcggatgc tgctcttccc
     4981 gcacgccgaa aaggttttcg acggcaccac cgcagccgtc ctgtcggggt tcaacaccgc
     5041 cacttccgga gtgccgaccg gagtcacgct ggtggccggc ccaggcgcca cggcggcctt
     5101 cgacagtcct gctcccggct ccaacgtcgg catcaccttt tccggctaca ccctggccgg
     5161 agcgaatgcg ggccaatata cgctcgccgg attctgctgc gtctccacgt tccggacgag
     5221 cggggcgata accgcggctg ccgcgccgcc accgcccgca ccaccacctc ctgcgccgcc
     5281 gcctcctgcg ccgccgcctc ccgcgccgcc gcctcccgcg ccgccgcctc c
//
LOCUS       DASWNU010000275         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_320602, whole genome shotgun sequence.
ACCESSION   DASWNU010000275 DASWNU010000000
VERSION     DASWNU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320602"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VGB57_05435"
     CDS             complement(<1..869)
                     /locus_tag="VGB57_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0130828.1"
                     /translation="MDLIAALVLAAAAPAPADCQTLLGSLYEQPEDADLPGLVSGETL
                     KDGKALIALRLARGDKAIPVRGGDFSGADFRGARLDRICFLGTKLTGSQMRGANLSGS
                     AFVGADLSGADLRETRMSRILLRNAVLKDADAQRSILKGGRFDGGWGEGSVEGFRLDS
                     ADLTGFRFDCGITLDDGCPIDGGRLTLSGAILRDSNLAAFVDVAGARIDRTRVSPSEL
                     PDLRNARLEGPVIVAGGDEEVPLSAEEYRSLLPYFRDPEPEEPPAGKGGERLRNVLSG
                     AVLYFVDHPQAFT"
     gene            complement(883..>1873)
                     /gene="rpoN"
                     /locus_tag="VGB57_05440"
     CDS             complement(883..>1873)
                     /gene="rpoN"
                     /locus_tag="VGB57_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY0130829.1"
                     /translation="GYLLGSLLEIAQRLGAPLGDVERVLGVIHGFDPPGVAARSLAEC
                     LALQAKEADRYDPAMARLIDNLDYLAKGNFSALKRICGVDDEDLGDMVRELRAYDPKP
                     GCRYAGGNDRIEAAMPDVFVARRGFGWAIELNSATLPRLLVNRRYYAELTGGADRRSK
                     AWLSECLASANWLMKALDQRARTIVRVCSEIVKQQDGFFRHGVAQLKPLTLRQVAEAI
                     GMHESTVSRVTSNKYLSCDRGLYELKYFFTSGIQSNDGGDAVSAEAVKSHIKALIAAE
                     DPRAILSDDQLVELLRAKGFDIARRTVAKYREAIGLGSSVQRRRQKAMEGRAA"
BASE COUNT          301 a          624 c          647 g          301 t
ORIGIN      
        1 tcggtgaagg cctgggggtg atcgacgaag tacaggaccg cgccggacaa cacgttcctg
       61 agccgctcgc cgcctttccc ggcggggggc tcctccggct cgggatcgcg gaaatagggc
      121 aggagagagc gatattcctc cgccgaaagc ggcacctcct cgtctccgcc ggcgacgatc
      181 accggccctt ccagccgggc attccgaaga tcggggagtt cggagggtga gaccctggtc
      241 cggtcgatcc gggcgccggc gacgtcgacg aaggcggcga ggttcgaatc gcggaggatc
      301 gccccgctca aggtcagccg tccgccgtcg atcgggcagc cgtcgtcgag ggtgattccg
      361 cagtcgaagc ggaagccggt cagatcggcg gagtcgagcc tgaagccctc gacgctgcct
      421 tccccccacc cgccgtcgaa ccggccgcct ttcaggatcg agcgctgggc gtccgcatcc
      481 ttcaataccg cgttgcgcaa caggatgcgg gacatgcgcg tctcgcgcag atcggctccg
      541 ctgaggtcgg ctccgacgaa ggcggagccc gaaagattgg cgccgcgcat ttggcttccg
      601 gtcagcttcg tgccgaggaa gcagatcctg tcgagccgag cccctcgaaa gtccgcaccc
      661 gagaaatcgc cgccgcggac cgggatcgcc ttgtcccctc tcgcaagccg aagcgcgatc
      721 agggccttgc catccttcag ggtctcgccc gagaccaggc cgggaaggtc ggcgtcctcc
      781 ggctgctcgt agagggaccc aagcagagtc tggcaatcgg ccggagcggg cgccgcggcg
      841 gcgagcacga gcgccgctat cagatccact gcgcgtccct cgtcacgccg cccggccctc
      901 catcgccttc tggcggcggc gctggacgga ggagccgagc ccgatcgcct cgcgatattt
      961 ggcgacggtc cggcgggcga tgtcgaagcc cttggctcgg agcagctcga cgagctgatc
     1021 gtcggacagg atggcgcgcg gatcctcggc ggcaatgagc gccttgatgt ggctcttcac
     1081 cgcttcggcc gagacggcgt cgccgccgtc gttggactgg atgccgctgg tgaagaaata
     1141 tttgagctcg tagaggccgc ggtcgcacga cagatatttg ttcgaggtga cccggctcac
     1201 cgtcgattca tgcatcccga tcgcttcggc cacctgcctc agggtgagcg gcttcaattg
     1261 ggcgactccg tggcggaaga agccgtcctg ctgcttgacg atctcgctgc acaccctgac
     1321 gatggtccgg gcgcgctggt ccagcgcctt catcagccag ttggcgctgg ccaggcattc
     1381 gctcagccag gccttggagc ggcggtccgc cccgccggtg agctccgcat aatagcggcg
     1441 gttgacgagg agccggggca gggtcgcgga gttcagctcg atcgcccagc cgaagccgcg
     1501 gcgggcgacg aacacgtcgg gcatcgccgc ctcgatccgg tcgttgccgc cggcgtagcg
     1561 gcagccgggc ttgggatcgt aggcccggag ctcgcgcacc atgtcgccga gatcctcgtc
     1621 gtcgaccccg cagatccgct tcagagccga gaaattgccc ttggcgagat agtcgagatt
     1681 gtcgatcagc cgggccattg cgggatcgta gcggtcggcc tccttcgcct ggagcgcgag
     1741 gcattcggcc agtgagcggg cggcgacgcc cggcggatcg aagccgtgaa taacgccgag
     1801 gacgcgttct acgtcgccga gcggagcgcc gagccgttgc gcgatctcga gcagcgatcc
     1861 aagcaaatat ccg
//
LOCUS       DASWNU010000276         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_193594, whole genome shotgun sequence.
ACCESSION   DASWNU010000276 DASWNU010000000
VERSION     DASWNU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193594"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            580..711
                     /locus_tag="VGB57_05445"
     CDS             580..711
                     /locus_tag="VGB57_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin EcnAB"
                     /protein_id="HEY0130830.1"
                     /translation="MRKLITLAFIATGLTLSACNTVRGAANDVESTTDCADGQPGNC"
     gene            complement(757..1395)
                     /locus_tag="VGB57_05450"
     CDS             complement(757..1395)
                     /locus_tag="VGB57_05450"
                     /inference="COORDINATES: protein motif:HMM:NF027558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEY0130831.1"
                     /translation="MRLRLVYCLAVLAGTAACRTPPPDRPAAAALRVVVEEPEAWRGI
                     ASPRDAAALDGLADRWRLALAAGRAANLSRRIAAEGDLLIPDTRLPRAAPAPGSYRCR
                     YVRPGGRRWVSSAPGFCYVGVEVGQLTLATELRGLRFGGYLWEVKGGERLVFLGGAVP
                     AGTRTPLAYGENPSRDASGLVERIGEFRYRLTLPEPSAGAGLTVVELIAAPR"
     gene            complement(1460..2284)
                     /locus_tag="VGB57_05455"
     CDS             complement(1460..2284)
                     /locus_tag="VGB57_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0130832.1"
                     /translation="MPVASVSEADLLTELQLAVERDELSLLYQPKVAVADNRLVGVEA
                     LARWPHRTLGMVEPSTFVPLAERYGLIDELTDWVLRRGLKQWVTWSEQGLRTNIAFNV
                     SALSLRDVYFPDYLHRLCQTEGVPANCLTIEVTEGATQHLVRLLDTLTRFRLKGMSLA
                     LDDFGTGYSSLLQLRQLPYSELKVDQCFVGEAVRSRESRLIVKAVIDLAHGLGLEATA
                     EGVEDAETLALLGELGCDHAQGFFIARAMEGWELAPWLMAADGEGAVPIASARRRG"
     gene            complement(2262..2717)
                     /locus_tag="VGB57_05460"
     CDS             complement(2262..2717)
                     /locus_tag="VGB57_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0130833.1"
                     /translation="MTEPLLPEPAVRPLMRAPATRRRLLLIDDELSVARFIAHAAEEC
                     GYEAIITVTAESFRSQYLACDPDVVVLDLALPRSDGVELLRFLAEEKSRALVLPISGF
                     DERVLEAAMRLGTALGLRMTGPLQKPVRLQQLMDAMQGREAQDAGCIGF"
     gene            complement(2959..3732)
                     /gene="tatC"
                     /locus_tag="VGB57_05465"
     CDS             complement(2959..3732)
                     /gene="tatC"
                     /locus_tag="VGB57_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY0130834.1"
                     /translation="MSEIDETRAPLMEHLVELRRRLIWSFVALGIAFGVSFYFAGRIF
                     GFLVQPLIAGFGTGQGRLVYTKLYEAFFTEVRVALFAAFFLAFPIIATQLWMFVAPGL
                     YAKEKRAFLPFLLATPVLFIGGGALAYYVVMPLAIKFFLGYQGQIPGIQQEALPAMGE
                     YLSFVMHFILAFGIAFLLPILLMLMERAGLVTRDQLKSGRRYAILVAFVIAAIATPPD
                     IVSQFLLAIPLILLYELSLIAIWFTERKRAKQAAVAPPA"
     gene            complement(3729..4094)
                     /gene="tatB"
                     /locus_tag="VGB57_05470"
     CDS             complement(3729..4094)
                     /gene="tatB"
                     /locus_tag="VGB57_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY0130835.1"
                     /translation="MFDIGYTELLVIAIVALVVIGPKDLPRVMRTVGQWVGRARGMAR
                     HFRSGIDTMMREAELEEMEKKWREENARILAEHPFAEPLADPEALAAPAELPAPDDLA
                     PEPERDGATPPPAAAGPLP"
BASE COUNT          722 a         1467 c         1396 g          645 t
ORIGIN      
        1 gagatcctcc gcgccctgcc ccgcctctac ggcgctttcg acattgagct tctcgtcccg
       61 gtggactccg tcgatgtcgc tgtgcggggt ggatttcggc ggctttgtca cgataagggt
      121 tcctcttcgg cccctgaaac cggcggaagc gcaggccggt ttcgaaaaaa atttcctgga
      181 atgcgatttt tttgggaacg gcgggaaccc ctccgcccct gcgccgttat gtagttcgga
      241 tagtgacctt ttgccccccc gctctcgcta tccacgacat gggcccggac gcgctaacct
      301 cccccccccc ggtagcgcct ccgggcccac attttttctc gtcattgcaa gcgaagcgaa
      361 gcaacccggt gcagcgtccg aggcccgctg gattgccgcg tcgcgtcgct cctcgcaatg
      421 acggtcagct ctcgggcccg ctcctaaccg attgctcgcc gaattcccgc gccctcggaa
      481 taacgagggc ggaacaatcg cgccgccccc cgcatttccc ttctaccttg cgacgacgga
      541 cgcgcgacgc gcctggcgga gcattcgagg agaccgaaca tgcgtaagct tatcaccctc
      601 gccttcatcg ccaccggcct gaccctcagc gcctgcaata cggttcgcgg tgccgcgaac
      661 gacgtcgaat cgaccaccga ctgcgccgac ggccagcccg gcaactgctg agccgcacgg
      721 cttttcgaaa aggccgcctc cgggcggcct tttttttcag cggggcgccg cgatcagttc
      781 gacgacggtg agtccggccc ccgctgacgg ttccggcagg gtgagccggt agcggaactc
      841 gccgatccgc tcgaccaatc cggaggcgtc gcgggacggg ttctcgccat aggccagcgg
      901 agtcctggtc ccggccggca cagccccgcc gaggaagacg agccgctcgc cgcccttcac
      961 ctcccacaaa tagccgccga agcgcaggcc gcgcagctcc gtagccaagg tgagctgccc
     1021 cacctcgact cccacgtagc aaaagcccgg agccgagctc acccatcgtc ggccgccggg
     1081 ccggacgtac cggcaccgat agcttcccgg cgccggcgcc gcccggggga gccgcgtatc
     1141 gggaatcagg agatcgcctt ccgcggcgat ccggcgcgac aggttcgcgg ctcgtccggc
     1201 cgcgagcgcc agccgccagc ggtcggccag gccgtccaga gcggccgcat cgcgcggcga
     1261 ggcgatgccg cgccacgcct ccggctcctc gaccacgacc ctgagcgcgg cggcggccgg
     1321 cctgtccggc ggcggcgtcc ggcacgcggc cgttccggcc aggacggcaa gacaatagac
     1381 aagacgcagc cgcatcgcga gaaccctccc cacccaattt tttaatgcca agcctacaaa
     1441 gcccgcccgc cggttggcgt cagcccctcc gcctcgccga agcgatcgga accgcgcctt
     1501 cgccgtccgc cgccatcagc cacggcgcca gctcccaccc ttccatcgcc cgggcgatga
     1561 agaagccctg ggcatggtcg cagcccagct ctccgagcag agccagggtc tcggcatcct
     1621 cgaccccttc cgccgtcgcc tcgaggccga gcccgtgggc gaggtcgatc accgccttga
     1681 cgatcagccg tgactcgcgc gagcgaacgg cctccccgac gaagcattga tcgaccttga
     1741 gctcgctata cggcagctgg cggagctgga gcagtgagga ataacccgta ccgaaatcgt
     1801 cgagcgcgag gctcatcccc ttcagccgga agcgggtcag ggtgtcgagc aggcgcacga
     1861 gatgctgggt cgctccctcg gtgacctcga tggtcaggca attggccggc acgccttcgg
     1921 tctggcagag ccggtgcaga taatcgggga aatagacgtc gcgaaggctc agcgccgaga
     1981 cgttgaaggc gatgttggtg cgaagtccct gctcgctcca ggtcacccat tgcttgagcc
     2041 cgcgccggag tacccagtcg gtcagctcgt cgatcaggcc gtagcgctcg gcgagaggaa
     2101 cgaaggtcga cggctcgacc atcccgagcg ttcggtgcgg ccaccgcgcc aacgcttcca
     2161 cgccgaccag ccggttgtcc gcgacggcca ccttgggctg gtagagcagg gacaattcgt
     2221 cccgctccac cgccagctgg agctcggtga ggagatccgc ctcagaaacc gatgcaaccg
     2281 gcatcctgcg cctcccgtcc ctgcattgcg tccatgagct gctggagccg gaccggcttc
     2341 tgcagcggcc ccgtcatccg aagcccgagc gcggttccaa gccgcatcgc cgcctcgagc
     2401 acgcgctcgt cgaagccgct gatcggaagg accagcgccc ggctcttctc ctcggcgagg
     2461 aagcgcagga gctcgacccc gtcgcttcga ggaagagcga ggtcgagcac gaccacgtcc
     2521 gggtcgcagg ccagatactg gctccggaag ctctcggcgg tcacggtgat gatcgcctcg
     2581 tagccgcatt cctcggcggc gtgggcgatg aagcgggcca ccgaaagctc gtcgtcgatc
     2641 aggagaagcc gccggcgggt cgcgggcgca cgcatcagcg gacggaccgc gggctcggga
     2701 aggaggggct cagtcatggc agtgggagtt gcggcgatcg gccatgtcgg cctcctttgc
     2761 ttcgccccct cccggccgac tcaacaccgg cataggttaa gaaagtctaa agaagcgcga
     2821 ttttaagcgt ggtcacgagg ccgagttgtt ggggaggtgc ggcgagtttc gtcgccccag
     2881 cgaaggctgg gggcccagcg cgtgaaaggc gccgcggtca tcaccccctg cggtcccagc
     2941 cttcgctggg acgacgcgct aggccggagg cgcgaccgcc gcttgcttcg cccgcttccg
     3001 ctcggtgaac cagatcgcga tcagcgacag ctcgtagagg aggatcagcg ggatcgcgag
     3061 gaggaattgg gagacgatgt cgggcggagt ggcgatcgcc gcgataacga aggcgacgag
     3121 gatcgcgtag cgccggccgc tcttgagctg gtcgcgggtg accagccccg cccgctccat
     3181 cagcatcagc aggatcggca ggaggaaggc gatcccgaag gcgaggatga agtgcatgac
     3241 gaaggagaga tattctccca tcgccggcag cgcttcctgc tggatccccg gaatttgccc
     3301 ctggtagccg aggaagaatt tgatcgcgag cggcatcacc acgtaataag cgagcgcccc
     3361 gccgccgatg aacagaaccg gggtggcgag caggaagggc aggaaggccc ttttctcctt
     3421 cgcgtaaagg ccgggcgcga cgaacatcca gagctgggtg gcgatgatcg ggaaggcgag
     3481 gaagaaggcc gcgaacagcg ccaccctcac ctccgtgaag aaggcctcgt agagcttggt
     3541 atagaccagc cgcccctgtc ccgttccgaa gcccgcgatc agcggctgga cgaggaagcc
     3601 gaagatccgg ccggcgaagt agaagctcac cccgaaggcg attccgagcg ccacgaacga
     3661 ccagatcagc cgccgcctta gctcgaccag atgctccata agcggagcgc gggtctcgtc
     3721 gatctcgctc atggcagcgg accggcggcg gcgggcgggg gcgtcgctcc gtcccgctca
     3781 ggctcgggtg ccaaatcgtc gggagcggga agctcggccg gcgcggccag cgcctcggga
     3841 tcggcgagcg gttcggcgaa cggatgctcg gcgaggatcc gcgcattctc ctcgcgccac
     3901 ttcttctcca tttcctcgag ctcggcctcc cgcatcatcg tgtctatgcc cgagcggaaa
     3961 tggcgggcca tgccgcgggc gcggccgacc cattggccga cggtgcgcat cacccgcggc
     4021 aggtccttgg gcccgatcac caccaaagcg acgatggcga tgacgagcag ctcggtatag
     4081 ccgatgtcga acatcaggcg cgcccttcgc gcgaggttct agggctcgcg tcgatcgccg
     4141 aggtccgcct cgcggctgcg ctcggcggga ggcgcgacgc tcgcctcgtc ggtggtgagc
     4201 cggcggggag gctccgcggg tctggcggcg
//
LOCUS       DASWNU010000277         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_61780, whole genome shotgun sequence.
ACCESSION   DASWNU010000277 DASWNU010000000
VERSION     DASWNU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61780"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="VGB57_05475"
     CDS             complement(<1..1726)
                     /locus_tag="VGB57_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0130836.1"
                     /translation="MVSRKAFLLFLLAGTATSPLLAQEAPAPETAVPTPAPVPPEPVQ
                     VPAEAEAAVQVDPEDPDAELDEEGEPIVVTGQRQRGAVIGDIQPEVQFDRREIRALGA
                     GSITELLEAIAPQTQSARGREGGRPVILLNGRRISGFAEIRDIPPEAIVRIDVMPEEV
                     ALKYGYRADQRVVNFVLRRRFNAYTAEVQGGLATEGGRESYGAELNYLQINQAGRINL
                     GAEFRRANPLFESERDIVQAEPVAGIELGRFRTLLPQTSQLELSGTINRAVSETVSAT
                     LNGTFDVNSSTAFLGLPRPSLTPPGLRRESDTRAAHLGLSVNGSIAPWQWTLTGNLDR
                     SANESETQTNAGLDRSRSVSQIGNAELLLNGPLFNLPAGGVTAAARAGFDARGLDSHS
                     DRGGFFTVRELSRTREHAQVNIDIPIASRRRAALTALGNLSANLNAEVEHLSDFGTLR
                     TLGAGLNWSPISQISLIASVTDEDGAPSMQQLGDPVQQTPGVRVFDFVRGETVEVTSI
                     SGGNPNLVEDNRRVWKLGANLRPFEDKDLSISANYTSSRIVNAISGFPAATAEIEAAF
                     PDRFQRGPD"
BASE COUNT          247 a          562 c          680 g          295 t
ORIGIN      
        1 cgtccgggcc gcgctggaag cggtcgggga aagccgcctc gatctccgcc gtggccgccg
       61 ggaagccgga gatggcgttg acgatccggc tggaggtgta attggcgctg atcgacagat
      121 ccttgtcttc gaacggccgg aggttggcgc ccagcttcca cacccgccgg ttgtcctcga
      181 ccaggttcgg gtttccgccc gagatgctgg tcacctcgac ggtctcgccg cgcacgaagt
      241 cgaagacccg cactccgggc gtctgctgca ccggatcgcc gagctgctgc atcgacggcg
      301 ccccgtcctc gtcggtgacg gaggcgatca ggctgatttg cgagataggg gaccagttga
      361 gcccggcgcc gagcgtccgc aaggtgccga agtcggacag atgctcgacc tcggcgttga
      421 ggttggcgga caaattgccg agcgccgtca gcgccgcccg gcgccggctg gcgatcggga
      481 tgtcgatatt gacctgggca tgctcgcggg tccgcgagag ctcgcggacg gtgaagaatc
      541 cgccccggtc ggagtggctg tcgaggccgc gggcgtcgaa gccggctcgg gcggcggcgg
      601 tgactccgcc ggcgggcagg ttgaacaggg gcccgttcag aagaagctcg gcattgccga
      661 tctggctgac cgagcgcgag cggtcgaggc cggcattggt ctgggtttcg gactcgttcg
      721 cgctccggtc gagattgccg gtcagggtcc attgccatgg cgcgatggag ccgttcaccg
      781 aaaggccgag atgggcggcg cgggtgtcgc tctcgcggcg aaggcccggc ggcgtgaggc
      841 tcggccgggg caggccgagg aaggcggtgc tcgaattgac gtcgaacgta ccgttcaagg
      901 tcgccgagac ggtctccgag acggcgcggt tgatcgtccc gctcagctcc agctggctgg
      961 tctgcggaag cagcgtgcgg aagcggccga gctcgatccc ggccaccggc tccgcctgga
     1021 cgatgtcgcg ctcgctctcg aacagggggt tggcgcggcg gaactcggcg ccgaggttga
     1081 tccggcccgc ctggttgatc tgcagatagt tgagctcggc gccgtaggat tcgcggccgc
     1141 cctcggtggc gaggccgccc tggacctcgg ccgtataggc gttgaagcgg cgcctcagga
     1201 cgaagttcac cacccgctgg tcggcccgat agccgtattt gagggcgact tcctccggca
     1261 tgacgtcgat ccggacgatc gcctcgggcg ggatgtcgcg gatttcggcg aagccggaaa
     1321 tccggcggcc gttgaggagg atgacggggc gcccgccctc gcggccgcgg gcgctttggg
     1381 tctgcggggc gatcgcctcg agcaattcgg tgatgctgcc ggcgccgagc gcccggatct
     1441 cgcggcggtc gaactgcact tcgggctgga tgtcgccgat caccgcgccg cgctggcgct
     1501 ggccggtgac gacgatcggc tcgccctcct cgtccagctc cgcgtcggga tcctcggggt
     1561 cgacctggac ggcggcctcc gcctccgccg gcacttgcac cggctccgga gggacgggag
     1621 cgggcgtggg gacggctgtt tcgggcgcag gggcttcctg ggcgaggagg ggagacgtcg
     1681 ccgttccggc gagaaggaag agaaggaagg ctttgcggga caccacgcga ctgacacctt
     1741 ttttgttttc acgacccgtc aggggttcgg ccggcgttgg cccg
//
LOCUS       DASWNU010000278         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_193655, whole genome shotgun sequence.
ACCESSION   DASWNU010000278 DASWNU010000000
VERSION     DASWNU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193655"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VGB57_05480"
     CDS             complement(<1..1492)
                     /locus_tag="VGB57_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY0130837.1"
                     /translation="MYIELQAATHFSFLRGVSSAEEMFAAAALLGYPALGIVDRNTVG
                     GLVKTLRAADDTGVRLIPGCRLDLMDGTGLLVWPEDRSGWSRLTRLLTAGKSRADRDK
                     GEKGQCFLHWEDVAAWNGGLVAALVPDEADESTGAALAQMRDIFGERAHLALTHRRRP
                     GDSARLQALDAMAKRHGVRALATGDVLYDSPDKRMLQDVVTAIRHKCTIDDLGFRRER
                     FADRHLKSPGEMERRFGDHPDSVRASADIAERCTFSLRELSYQYPDEIVMSGRTPQEA
                     LERLTRTALASKFPEGPPQSHVDLLEKELGLVEKLAYAPYFLTVNSVVQFARSQEILC
                     QGRGSAANSMICFVLGITSIDPVKHTLLFERFISESRNEPPDIDVDFEHERREEVIQW
                     IYDHYGHSHAALTAVVSRYRTRGAVREVGKALGLPEDLTAMLSSQVWGWSEAGVPERH
                     VDELGLDRNDPRLALTLELTRQLVGTPRHLSQHPGGFVLTRDRLDDL"
     gene            complement(1668..>3058)
                     /locus_tag="VGB57_05485"
     CDS             complement(1668..>3058)
                     /locus_tag="VGB57_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEY0130838.1"
                     /translation="VSQARALVPGLDIRDADPEADAGVLTRLALFAARRWTPRAAVSP
                     PDGLFLDLTGVAHLFGGEERMCRRIVAFCARAGVEARIAVAGTPGAAHALARFGPGTI
                     CPVNGEPEALAPLPLAALRVEEPVLAAARRLGLETIGDLLPLPRGPLQRRFGKTLLAR
                     LDQALGRSGEPIEPVVPEEAPCAVLRFAEPIATAEAIAGALALLMERLIETLREQGLA
                     ARTLALLCDRVDGKVERIAIGTARATRDGGHLLRLLGMKIETIEPGFGLDEMRLVAGR
                     VEPLAPEQPSLRGAQRRSNPAGLGDPAGLLRSARNDEEADLAMLVDRLAGRLGLSRLY
                     RATAVESDVPERSVARTGPLDSAVPWPKWPRPVRLLSPPERVENVVALLPDLPPRRFT
                     WRGRAYRVARADGPERIHGEWWKRAAEAEGVRDYFQVEDEEGARFWLYRRGDGVDART
                     GDLTWYLHGVFG"
BASE COUNT          478 a         1004 c         1135 g          441 t
ORIGIN      
        1 cgagatcgtc gagccggtcg cgggtgagga cgaagccgcc gggatgctgg ctgaggtgcc
       61 tcggggtgcc gaccagctga cgggtgagct cgagcgtcaa agcgaggcgc ggatcattgc
      121 gatcaaggcc cagctcgtcg acatggcgct caggaacgcc cgcttccgac cagccccaga
      181 cctggctcga cagcatggcg gtgagatcct ccggcaatcc cagcgccttg ccgacctcgc
      241 ggaccgcgcc gcgggtccgg tagcggctga ccaccgcggt caaggcggcg tgggagtggc
      301 cgtaatggtc gtagatccac tggatcactt cctcgcgccg ctcatgctcg aaatcgacgt
      361 cgatgtcggg cggctcgttg cggctttccg agatgaagcg ctcgaagagg agggtgtgct
      421 tgaccggatc gatcgaggtt atgccgagca cgaagcagat catcgaattg gccgccgagc
      481 cgcggccctg gcacaatatc tcctgactcc gggcgaactg gacgaccgag ttcacggtaa
      541 ggaagtaagg cgcgtaggcg agcttctcga cgagccccag ctccttttcg agcaggtcga
      601 catggctttg cggcgggcct tcggggaatt tggaggccag agcggttcgg gtcagccgct
      661 ccagcgcctc ctgcggggtg cggcccgaca tcacgatctc gtcgggatat tgatagctca
      721 attcgcgaag cgagaaggtg cagcgttccg cgatgtcggc cgaggcgcgg accgaatcgg
      781 gatgatcgcc gaagcgccgc tccatctccc cgggcgactt caggtggcgg tcggcgaagc
      841 gctcgcggcg gaagccgaga tcgtcgatcg tgcatttgtg gcggatcgct gtgacgacgt
      901 cctggagcat tcgcttgtcg ggcgaatcgt agagcacgtc gccggtggcg agggcccgca
      961 cgccgtggcg cttcgccatc gcatcgagcg cctggagacg cgccgaatcg cccggccggc
     1021 ggcgatgggt gagggcgaga tgggcgcgct cgccaaagat gtcgcgcatc tgggcgagcg
     1081 cggcgccggt cgattcgtcg gcctcgtccg gcaccagggc cgcgaccagg ccgccgttcc
     1141 aggcggcgac gtcctcccag tggaggaagc attgcccctt ctcgcccttg tcgcggtcgg
     1201 cgcggctctt gccggcggtg agcaggcggg tgaggcgcga ccagccggag cgatcctcgg
     1261 gccagacgag gaggccggtg ccgtccatca ggtcgagccg gcagccgggg atcaggcgta
     1321 caccggtgtc gtcggcggcc ctcagcgtct tgacgaggcc gccgacggtg ttgcggtcga
     1381 cgatgcccag cgccggatag ccgagcaagg cggcggccgc gaacatctcc tccgcgctcg
     1441 agaccccgcg aaggaaggag aaatgggtcg cggcctggag ctctatgtac attatgcctc
     1501 cccatcgcag atggggaggg ggaccacctg aaaggtggtg gaggggtaat tggcagaagc
     1561 aatcgaggtt ttgcgcgcga acctcttggt cccgcgccag ctacccctcc accatgcttc
     1621 gcatggtccc cctccccccg cttcgcgcgg ggaggcaaaa accgcgctca tccgaacacc
     1681 ccgtgcaggt accaggtcag atcgcccgtc cgggcatcca ctccgtcgcc cctccggtag
     1741 agccagaacc tcgccccctc ctcgtcctcg acctggaaat aatcgcgaac gccttccgct
     1801 tcggcggcgc gcttccacca ttcgccgtgg atccgctcgg ggccgtcggc gcgggcgacc
     1861 cggtaggcgc ggccgcgcca ggtgaagcgg cggggcggaa gatcggggag caaggcgacg
     1921 acgttctcga cccgctcggg cggcgaaagg aggcggacgg ggcgcggcca tttcggccag
     1981 ggcacagcgg agtcgagcgg gccggtgcgg gcgacgcttc gctcggggac gtcgctctcg
     2041 accgcggtgg ctcggtagag ccggctcagg ccgagccggc cggcgaggcg gtcgacgagc
     2101 atcgcgagat cggcctcctc gtcattgcga gcggagcgaa gcaatccagc cgggtcgccg
     2161 aggcccgctg gattgcttcg tcgctgcgct cctcgcaatg acggttgttc gggcgcgagc
     2221 ggctcgaccc ggccggcgac gaggcgcatc tcgtcgaggc cgaagccggg ctcgatcgtc
     2281 tcgatcttca ttccgagcaa tcgaagcaga tggccgccgt cccgggtcgc cctcgccgtt
     2341 ccgatggcga tccgctccac tttcccgtcg acccggtcgc agagcaaggc gagagtccgc
     2401 gccgccagcc cctgctcccg gagcgtctcg atcaggcgct ccatcagaag ggcgagcgcc
     2461 cctgcgatcg cctccgcggt ggcgatcggc tcggcgaagc gaaggacggc gcaaggcgcc
     2521 tcctccggaa ccaccggctc gatcggctct ccggagcggc ccagcgcctg gtcgagtcgg
     2581 gcgagcaggg tcttgccgaa gcggcgctgg agcgggccgc gcggcaaggg gagaagatcg
     2641 ccgatcgtct cgaggccgag gcggcgggcg gcggcgagga ccggctcctc gacccgaagg
     2701 gcggcgagcg ggagtggggc cagagcctcg ggctcgccgt tgacgggaca gatcgtgccc
     2761 gggccgaagc gggcgagggc gtgggccgcg cccggcgttc cggcgacggc gatccgggct
     2821 tcgacgccgg cccgggcgca gaaggcgacg atccggcggc acatcctctc ctcgccgccg
     2881 aacaggtggg cgactccggt gagatcgagg aacagtccgt cggggggaga gactgcggcg
     2941 cgcggagtcc agcggcgggc ggcgaagagg gcgaggcggg tgaggacgcc ggcatccgct
     3001 tcgggatcgg cgtcgcgaat gtcgagaccc gggacgagag cccgggcctg ggagaccg
//
LOCUS       DASWNU010000279         7505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_61919, whole genome shotgun sequence.
ACCESSION   DASWNU010000279 DASWNU010000000
VERSION     DASWNU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61919"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VGB57_05490"
     CDS             <1..357
                     /locus_tag="VGB57_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130839.1"
                     /translation="PRRMVTVSSAGADCGSSNFYLRTKGEMEAALEGMGFERLDILQP
                     GLLLGPRGGDRRIGERIAIALSPLTNLLLRGRLDRYSAIGADVVAAAVAGALRQVESG
                     IFRHENRSIRRLAGRS"
     gene            420..2144
                     /gene="argS"
                     /locus_tag="VGB57_05495"
     CDS             420..2144
                     /gene="argS"
                     /locus_tag="VGB57_05495"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0130840.1"
                     /translation="MTLYARFAAHLDAILDALEGEGALPSGLERKAVAVEPPRDPSHG
                     DLATNAAMVLAKGARTNPRALAELIAPRLEALDEVVGAEVAGPGFINIRLSAEIWQDE
                     LRSILSEGAAYGRSSAGAGRRINVEYVSANPTGPMHMGHCRGAVVGDALASLLETAGY
                     AVTREYYVNDAGAQVDVLARSAHLRYLEALGRQIGEIPEGLYPGDYLKPVGHALAADF
                     SDRFADAPESEWLELFRKRTVEYMILLIKEDLAKLGVHHDLFSSELEVQNSGAVDRAF
                     ALLAEKGLVYRGTLPPPKGKTAEDWEPTELLLFRSTQYGDDQDRPIKKSDGSWTYFAA
                     DTAYHMRKLEASDELINVWGADHTGTVKRTQAAVQALAGRPALDVKLVQMVRLLRAGE
                     LVKMSKRSGSFVTLAEVVEEVGKDVVRFTMLTRKNDAQMDFDFAKVVEASKDNPVFYV
                     QYAHARIRSMLRKTPGLGIDMSVPADLSLLDDADWELVKLATQFPRLVEAAAQAHEPH
                     RIAFYLHDFSSAFHSRYNQGNDDPAQRFIVEDNQELTRARLELASAIGQIIRNGLGIM
                     GVTAAEEM"
     gene            2147..2854
                     /locus_tag="VGB57_05500"
     CDS             2147..2854
                     /locus_tag="VGB57_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0130841.1"
                     /translation="MTDIRADDPALDNDDRLPWLEAVEEDGGEGGPSALKLIVAVLIG
                     LAAIGGIVGGIFWMSNRDAAENGAPEVIAAPNGPYKVKPDSPGGMTVEGQGETAFDAA
                     QGGTPNASIDTGAVAEAPVTAQPKAQAPAPSEPPLLKAPAASNAPAAKADTPAAPAPA
                     AGGATIQLGAFPSGAAAEKAWKALSGRFAYLAPLSHNVVVAQVNGKTYYRLRASGPGA
                     AGICGRLKVAGEQCLKV"
     gene            2991..3998
                     /gene="nagZ"
                     /locus_tag="VGB57_05505"
     CDS             2991..3998
                     /gene="nagZ"
                     /locus_tag="VGB57_05505"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY0130842.1"
                     /translation="MQAAIYAPSGLELTPDERAFFRDADPVGYIVFRRNITNREQMRA
                     LTSELRDLHGRDDLPILIDQEGGRVARMRPPEWPAFPAAEAFARLYEKAPMSAIEAAR
                     ANAAAIAVLLAEVGVNVDCLPVLDVRQPGATDIVGDRALGSEPMQVAALGRAALDGLA
                     SQGVVGVVKHMPGHGRALVDSHKEMPVVGASEEALEIDLEPFRTLNRSPMGMTCHCVF
                     TAWDPERPASQSPAVIADVIRGRIGFDGFLMSDDIGMHALAGGFADRARRVLEAGTDA
                     VLHCSGAMEEMVAVAGSAGELGEAGLERLERALASVAGTGNGPSYAELAEKRDRLLAL
                     V"
     gene            complement(4064..4606)
                     /locus_tag="VGB57_05510"
     CDS             complement(4064..4606)
                     /locus_tag="VGB57_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0130843.1"
                     /translation="MLGPTLETPRLILRPPEERDLDGFAALMADEESARFIGGQEGRS
                     GAWRGMAAMAGSWALRGYGMFSVIEKESGKWIGRVGPWRPEGWPGAEVGWGLERSAWG
                     KGYATESAAAAIDWAFEALGWDSVIHCIAPANRPSARVATRLGSAVRGSAYLPAPFDS
                     EPVDIWGQTREQWRSNQGGR"
     gene            complement(4746..6722)
                     /locus_tag="VGB57_05515"
     CDS             complement(4746..6722)
                     /locus_tag="VGB57_05515"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEY0130844.1"
                     /translation="MRTTLLLASGLLALSLAAPGFAQPAPKGANKPRGSLTAFRSDAE
                     LRAYLRRLKRQEEAELPVPVPVMPEPMYAPPPPPPSAPPAAGAPAPNAVVVTGAPVAA
                     PAAGKSADSITNNQEAAVDEGGIVKMRGDTLVVLRRGRLFTISLAGGGMRPVDSIDAF
                     PPGVSGEGDWYDEMLISGDRVIVVGYSYDRGGTEINRFRLDPAGRLRFEDAWHLKSND
                     YYSSRNYASRLIGNRLIFYTPLELYWDDDQDLFDVLPGIRRWQGGPEAEKRKFKRIAR
                     ASRVFIAPAMRKSTDAVVDTLHSVTTCDLTAADLGCSATAVLGPESRTFYVSAQGVYL
                     WVTDAWKSDRPESYLYRLPFGRERPSATAARGAPVDQFSFREDPAEGILNVLVRSDGG
                     GDAMFAPENSGGGAGVALLRVRFSDFGDASRQLPRARYRFLPSVPGWSFHNRFAGDYV
                     LYGAGTYGEAARRANLVATRLRDGRATQLPVPHSVDRIELLGRDAMVIGGAQRGLGFS
                     TVELDGGEPDIGDRYVHPDSAEGESRSHAFFFSPDPDAPDGASGTVGLPVARRADPAL
                     ARFFGSSAAMVFLRRDDRRLAPAGELGAQTSGVVEDDCKASCVDWYGNARPIFWGGRT
                     FALLGYELVEGALARGRIREVGRISFAPAGPARQ"
     gene            6853..>7505
                     /locus_tag="VGB57_05520"
     CDS             6853..>7505
                     /locus_tag="VGB57_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY0130845.1"
                     /translation="MGGDESFDEFDSPAPVREADRLTLDLDGWEGPLDLLLTLARSQK
                     VDLAKISILALVEQYLAFIADAKKLKLEIAADYLVMAAWLAYLKSCLLLPKEVEADPT
                     PETLALRLQLRLQRLSAMREAGARLLGRDRIGRDVFLRGAPEGLRVIRKAAWQAELYD
                     LISAYGAIRARNEPVVHIVSRRPVMTLDEALTRVERLLGITVEWSELRSFLPETQDL"
BASE COUNT         1199 a         2531 c         2627 g         1148 t
ORIGIN      
        1 ccccgccgga tggtaacggt ctcgtcggcg ggagcggatt gcggatcgag caatttctat
       61 ctgcgcacca aaggcgagat ggaagcggcg ctggagggga tgggcttcga gcggctcgac
      121 atcctgcagc cgggcctgct gcttggtccg cgcggcggcg accgccgtat cggcgagcgg
      181 atcgccatcg ccctgagccc gctgacgaac ctgctcctcc gcggccggct cgatcgctac
      241 tcggcgatcg gcgccgatgt cgtcgcggcg gccgtggccg gagcgctgcg ccaggtcgag
      301 tcgggtattt ttcggcatga gaaccggtcc atccggcggc tggcggggcg gtcctagtcc
      361 gccttcaaat cgccgcggac atcgtctaag ccgcgctctc ctccacatcc ggacccgccg
      421 tgacccttta cgcccgcttc gccgcgcatc tcgacgccat cctcgacgcc ctcgaaggcg
      481 agggcgcgct gccgtccggg ctggagcgca aggcggtggc ggtggagccg cctcgcgacc
      541 cctcccacgg cgacctggcc accaacgcgg ctatggtgct cgccaagggc gccaggacca
      601 atccgcgcgc cctggccgag ctcatcgcgc caaggctgga ggcgctggac gaggtggtgg
      661 gcgccgaggt cgccgggcca ggcttcatca acatccgcct ctcggcggaa atctggcagg
      721 acgagcttcg ctccatcctg tccgagggcg ccgcttacgg ccgctcttcg gcgggcgcgg
      781 ggcggcggat caatgtcgaa tatgtctccg ccaacccgac cgggccgatg catatgggcc
      841 attgccgggg cgccgtcgtc ggcgacgctc tggcgagcct gctcgagacg gcgggctacg
      901 cggtcacccg cgaatattat gtcaacgacg cgggcgcaca ggtcgacgtc ctcgctcgct
      961 cggcgcacct gcgctatctc gaagcgctcg ggcgtcagat cggggagatc cccgaaggcc
     1021 tctatccggg cgactatctg aagccggtgg gacacgcgct cgccgcggat ttcagtgatc
     1081 gcttcgccga cgctccggaa agcgagtggc tcgagctctt cagaaaacgg acggtcgagt
     1141 acatgatcct ccttatcaag gaagatctgg cgaagctggg ggtccatcat gacctgttct
     1201 cctcggagct ggaagtgcag aattccggag ccgtcgaccg cgccttcgcc ctgctcgccg
     1261 agaaagggct ggtctatcgc ggcacgcttc ctccgcccaa aggcaagacc gcggaggatt
     1321 gggagcccac cgaattgctc ctcttccgct ccacccaata tggcgacgac caggaccggc
     1381 cgatcaagaa gtccgacgga agctggacct atttcgccgc cgacaccgcc tatcacatgc
     1441 ggaagctcga agcctcggac gagctgatca acgtctgggg cgccgaccat accggcacag
     1501 tgaagcggac ccaggcggcg gtgcaggcgc tggccggccg gccggcgctc gacgtcaagc
     1561 tcgtccagat ggtccggctg ttgagggccg gcgagctggt caagatgtcg aagcgctccg
     1621 gaagcttcgt taccctcgcc gaagtggtcg aggaggtcgg caaggacgtc gtccgcttca
     1681 ccatgcttac gaggaagaac gacgcccaga tggacttcga cttcgccaag gtggtcgagg
     1741 cctccaagga caatcccgtt ttctacgtcc aatatgccca tgcccggata cgctcgatgc
     1801 tgcgcaagac gcccgggctg ggcattgaca tgagcgttcc tgcagacttg tcgcttctcg
     1861 acgacgccga ctgggagctg gtgaagctgg ccacgcaatt cccccgcctc gtcgaggccg
     1921 cggcgcaggc gcacgagccg caccggatcg ccttctacct ccacgacttc tcgtcggcgt
     1981 tccactcgcg ctacaatcag ggcaatgacg atcccgcgca gcgctttatt gtcgaagata
     2041 atcaggagtt aaccagagcg cggctagaac tcgcctccgc aatagggcag attatccgca
     2101 acggcctcgg catcatgggt gtgaccgccg cggaggagat gtagcaatga ccgacattcg
     2161 cgccgacgat cctgcgctcg acaatgacga tcgtctcccc tggcttgagg cggtcgagga
     2221 agatggcggc gagggcggcc cctccgcgct caagctgatc gtcgccgttc tgatcggcct
     2281 cgcggcgatc ggcggaatcg tcggcggaat cttctggatg agcaatcgcg acgcggccga
     2341 gaatggcgcg cccgaagtga tcgccgcgcc caatggtccc tacaaggtca agcccgacag
     2401 cccgggcggg atgaccgtcg aaggccaggg cgaaacggcg ttcgacgccg cccagggcgg
     2461 cacccccaat gccagcatcg acaccggcgc ggtggccgaa gcgccggtga cggcccagcc
     2521 caaggcgcag gctcccgcgc cgagcgagcc gccgctgctc aaggccccgg ccgcgagcaa
     2581 cgccccggcc gcgaaggcgg acacgcctgc agctccggcg ccagccgcgg gcggcgcgac
     2641 gatccagctc ggcgctttcc cgagcggcgc ggccgctgaa aaggcctgga aagccttgtc
     2701 cggccgcttc gcctacctgg cgccgctctc gcacaacgtc gtcgtcgccc aggtcaacgg
     2761 caagacctat taccggctcc gcgccagcgg cccgggcgcg gccggaatct gcggacgcct
     2821 gaaggtggcc ggggagcagt gcctcaaggt ttaggaaggt gcgtcgcccc agcgaaggct
     2881 ggggccccag agggtgaata ccgctcgcgc ctttcacccg ccgggatccc agccttcgct
     2941 gggatgacgg ggcggctgac gcgcggctcg ggacgcgcta gggcttgcgg atgcaagccg
     3001 ccatttacgc gccttcgggg ctcgagctga ccccggacga acgggccttc ttccgcgacg
     3061 ccgatcccgt cggatatatc gtcttcaggc gaaacatcac gaaccgcgag cagatgcggg
     3121 ccctgacgtc ggagctgcgc gacctccacg gccgcgacga cctacccatc cttatcgacc
     3181 aggaaggcgg acgggtggcc cggatgagac cgcccgaatg gcccgctttc cccgccgccg
     3241 aggcgttcgc gcggctttac gagaaggcgc cgatgagcgc gatcgaggcg gcgcgggcca
     3301 atgcggcggc gatcgcggtc ctgctcgccg aagtcggggt caatgtcgac tgcctgccgg
     3361 tgctcgacgt tcgccagccg ggcgccaccg acatcgtcgg cgaccgggcc ctgggctcgg
     3421 agccgatgca ggtggcggcg ctcgggcggg cggcgctcga cgggctggcc tcgcaagggg
     3481 tggtgggggt ggtcaagcat atgccggggc atggccgggc gctggtcgac agccacaagg
     3541 aaatgccggt ggttggcgcg tccgaggagg cgctggagat cgacctcgag ccgttccgaa
     3601 cgctcaaccg gtcgccgatg gggatgactt gccactgcgt cttcaccgcc tgggatcccg
     3661 agcgcccggc gagccagtcg cctgcggtga tcgccgacgt gatccggggc cggatcggct
     3721 ttgacggatt cctgatgtcc gacgacattg gcatgcacgc tctggccggc ggcttcgccg
     3781 accgggcgcg gcgcgtgttg gaggcgggca ccgacgcggt cctgcattgc tccggggcca
     3841 tggaggagat ggtcgcagtg gccggcagtg cgggtgagct tggcgaggcg gggctcgagc
     3901 ggctcgagcg agcgctggcg agcgtggcgg gtacgggaaa tggcccgtcc tatgcggagc
     3961 ttgccgagaa gagggaccgg ctgctggctc tggtctagag ccacgtgcgc taatccgcct
     4021 catctcgtca tgccggcttg atccggcatc caccttctat tccttagcgg ccgccttggt
     4081 tcgaccgcca ctgctcgcgg gtctggcccc agatatcgac cggctcgctg tcgaacgggg
     4141 cgggcaggta ggcgcttccg cgaacggcgg agccgaggcg tgtcgcgacc cgggcggacg
     4201 ggcggttggc gggggcgatg cagtgaatga ccgagtccca gccaagcgcc tcgaacgccc
     4261 agtcgatcgc cgcggcggcg ctttcggtgg catagccctt gccccaggcc gagcgctcca
     4321 ggccccagcc gacctcggcg ccgggccagc cttccggccg ccaggggccg acccggccga
     4381 tccactttcc gctctctttc tcgataacgg agaacatccc gtagccccgc aaggcccagc
     4441 ttccggccat cgccgccatg ccgcgccagg cgcccgagcg tccctcctgc ccgccgatga
     4501 agcgcgcgct ttcctcgtcc gccatcagag cggcgaaacc gtcgaggtcg cgctcctcgg
     4561 ggggacggag gatcaggcgg ggggtctcga gggtcgggcc gagcatggat aggcctcttg
     4621 gagcctggca tggagcaagg caaccgtcat cccggcggaa gccgggacct cgtggagaat
     4681 agtctcggcg ggtctccccg aggtcccggc tttcgccggg atgaccggtg gggccgcgat
     4741 gacggctatt gtcgggccgg acctgcaggc gcgaagctga tccggccgac ctcccggatc
     4801 cgcccccgtg cgagcgcgcc ctcgaccagc tcgtagccga gcagggcgaa ggtccggccg
     4861 ccccagaaga tcggcctcgc attgccgtac cagtcgacgc agctcgcctt gcaatcgtcc
     4921 tcgacgacgc cgctagtctg agcgccgagt tcgccggccg gggcgagacg gcggtcgtcc
     4981 cggcggagga agaccatcgc cgccgagctt ccgaagaagc gggcgagcgc cggatccgcg
     5041 cgcctggcga ccggaaggcc gaccgtgccc gaggcgccgt ccggagcgtc cggatccggg
     5101 gagaagaaga aggcgtggct ccggctttcg ccttcggccg aatccgggtg gacgtaacgg
     5161 tcgccgatgt ccggctcgcc gccgtccagc tcgaccgtcg agaaaccgag tccgcgctgg
     5221 gcgccgccga tcaccatcgc gtcgcgcccg agcagctcga tccggtcgac cgaatggggc
     5281 accggaagct gggtggcccg gccgtcgcgc aggcgcgtgg cgaccagatt cgcccgcctg
     5341 gccgcctcgc cgtaggttcc ggcgccgtag agcacgtaat cgccggcgaa gcggttgtga
     5401 aacgaccatc ccggcaccga aggcaggaag cggtagcggg ctcgcggcag ctgccgcgaa
     5461 gcgtcgccga aatcggagaa gcggacccgg agcagcgcga ctccggcgcc gccgccgctg
     5521 ttctcgggcg cgaacatcgc gtcgccgccg ccgtcgctcc ggacgaggac gttgaggatg
     5581 ccttccgccg gatcctcgcg gaaggagaat tggtcgacgg gcgcgcctcg ggcggcagtc
     5641 gcggatggcc gctcgcggcc gaacgggagc cgatagagat agctttccgg acgatccgac
     5701 ttccaggcgt cggtgaccca cagatagacg ccctgggcgg agacgtagaa ggtccgcgat
     5761 tcgggaccga gcaccgccgt ggccgagcag ccgagatcgg cggcggtgag atcgcaggtg
     5821 gtgacgctgt gcagggtgtc gacgacggcg tcggtcgact tgcgcatcgc cggcgcgatg
     5881 aacacccggc tcgcgcgggc gatgcgcttg aacttgcgct tctcggcctc ggggccgccc
     5941 tgccagcggc ggatcccggg caggacgtcg aacaggtcct gatcgtcatc ccagtagagt
     6001 tcgagcggtg tgtagaagat cagccgattg ccgatcagcc gcgaggcgta gttacgcgac
     6061 gaataataat cgttggattt gaggtgccag gcgtcctcga agcgcagccg gccggcggga
     6121 tcgaggcgga agcggtttat ctcggtgccg ccgcggtcgt agctgtagcc gacgacgatc
     6181 acccggtcgc cggagatcag catctcgtca taccagtcgc cctcgcccga gactcccggc
     6241 gggaaggcgt cgatcgaatc gaccggccgc attccgccgc cggccagcga gatggtgaac
     6301 agccggccgc ggcgaaggac gacgagcgtg tcgccgcgca tcttgacgat cccgccctcg
     6361 tcgacagcgg cttcctggtt gttggtgatg ctgtccgccg acttcccggc ggcaggcgcg
     6421 gcgaccggcg cgccggtgac gaccaccgcg ttcggcgccg gagcgcctgc ggcgggcggc
     6481 gccgagggcg ggggaggcgg cggcgcgtac atgggctcgg gcatcaccgg cacgggcacg
     6541 ggcagctcgg cctcctcctg ccgcttgagg cgccgcagat aggcgcggag ctccgcatcg
     6601 gagcggaagg cggtgaggct gccgcgcggc ttgttcgccc cctttggcgc cggctgggcg
     6661 aagccgggag cggcgagtga gagagcgagc aggcccgagg cgaggagcag agtggtacgc
     6721 atggctctcc cagactttcg tgatagatat tttgtatcat cgaccgcaag cccggcaagg
     6781 gccaaaaaat gctgtgcata aatggggcga ttccccttcg cttcggccac ccgagccttt
     6841 agcctcgcgg ccatgggcgg agacgaatcc ttcgacgagt tcgactcgcc ggcgccggtg
     6901 cgggaggcgg accggctgac cctcgacctc gacggctggg aagggccgct cgaccttctg
     6961 ctgaccctgg cgcgctcgca gaaggtcgac ctggcgaaaa tctcgatcct cgctctggtc
     7021 gagcaatatc tcgccttcat cgccgacgcg aagaagctga agctcgagat cgctgccgat
     7081 tatctggtca tggcggcgtg gctggcttac ctcaaatcct gcctgctcct gcccaaggag
     7141 gtggaagcgg atcccacgcc ggagacgctg gcgctccggc ttcagcttcg gctgcagcgg
     7201 ctgagcgcaa tgcgcgaagc cggcgctcgg ctgctcggcc gcgaccgtat cggccgggac
     7261 gtcttccttc gcggggcgcc ggaagggctt cgggtcatcc gcaaggcagc gtggcaggcc
     7321 gagctctacg atctcatttc tgcctacggc gctattcgcg cccgcaacga gccggtcgtc
     7381 cacatcgtca gccggcggcc ggtgatgacg ctcgacgaag ccctgacgcg ggtcgagcgc
     7441 cttctcggca tcaccgtcga atggtcggaa ctacgaagct tcctgcccga aacccaggat
     7501 ttgga
//
LOCUS       DASWNU010000280         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_193791, whole genome shotgun sequence.
ACCESSION   DASWNU010000280 DASWNU010000000
VERSION     DASWNU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193791"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1487)
                     /locus_tag="VGB57_05525"
     CDS             complement(36..1487)
                     /locus_tag="VGB57_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY0130846.1"
                     /translation="MNSSTAALAKLYISDLNVRRTDRALDIDALADDIAAHGLIEPLV
                     VVDEGKGRGRFGVIAGGRRLLALQLLDKTARWRSTAGKVDPSAIPIQVREVEEGREIS
                     LSENVHKVAMNPADEVEAYAAIVADYEARGERDPAVRLARCARHYGKTVHYVEQRLRL
                     AGLVPEALEALRAGRITLDSAKAYASVGDQEVQRQIFAAQEREGKHSVAGIRSAVAGR
                     IYRATDRQVRYVGVEAYVAAGGRLDRDLFMGAEDGEVILDTGLLDRLATQKGEGEALR
                     VAAAAGYGGAVLKAWGGGAWQWPKTPEGLQRVYSLAGLGPDERASAFVICAIAADGAS
                     VELTDEGFKLAAAPAADAGAPPTRRPGSFLPLKDGESEVERLARIRRRKIEAAAVRLA
                     VPAFEGTALEGRTHWPTEGQTSIPALQEDDEFYAVAVYVKVSKAEVGRRMAEAEAIVD
                     QEFGGPPPRPPRIDDSKDWPEEAPARELAETMS"
     gene            complement(1842..2084)
                     /locus_tag="VGB57_05530"
     CDS             complement(1842..2084)
                     /locus_tag="VGB57_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEY0130847.1"
                     /translation="MAEGTIAADELRLLIERIERGEEEKKAVADDVKDVYAEAKSRGY
                     DTKTIRTIVRLRKMEKHDRQEADALLGAYKAALGME"
     gene            complement(2077..2466)
                     /locus_tag="VGB57_05535"
     CDS             complement(2077..2466)
                     /locus_tag="VGB57_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130848.1"
                     /translation="MAEELPPLAAQRWVFDARRNLVTNESNKGYVVASVGSGGPAADR
                     NLNGRAIAAVPSLVKAIELGLPALRNELACNLSGHCLSDAEGRPDRASLDEDVEEVVE
                     ALEAAIAAAEQALTAIAFPSCAEIPHG"
     gene            complement(2604..2951)
                     /locus_tag="VGB57_05540"
     CDS             complement(2604..2951)
                     /locus_tag="VGB57_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130849.1"
                     /translation="MTAPAAQPAAELDDPRLAEWAGPELLKALQVAVTAMKRQLGGLL
                     EIGCLHSAAGPLRSTLDLDLVDEVADLEDAIEGGELAIRKATTLPQNLSAADQAALIA
                     RFSPPQIEELQHG"
     gene            complement(2948..3106)
                     /locus_tag="VGB57_05545"
     CDS             complement(2948..3106)
                     /locus_tag="VGB57_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130850.1"
                     /translation="MRHDPNLARLAAPARLAEARARRAFDRACILLGLLALALVPGRA
                     LLAWAWPS"
     gene            complement(3096..3566)
                     /locus_tag="VGB57_05550"
     CDS             complement(3096..3566)
                     /locus_tag="VGB57_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130851.1"
                     /translation="MTETPIAAAMAPGTYIRKRREAAGQTLDQVVSWFADDGSGITRG
                     ELMAQLRALESGTAPVMTLSVEPLKGAFPFDPNVYLALVFFASGAKVVLPPICRDCAC
                     SEFDPCHDQCGDPCDWARVGVDEAPLCTMCIPDPAEGDVLQNPVNVGGEVRDAA"
     gene            complement(3701..>4175)
                     /locus_tag="VGB57_05555"
     CDS             complement(3701..>4175)
                     /locus_tag="VGB57_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130852.1"
                     /translation="RLAERIGWDQAAELVCKSPSTLRSWGDPGYGPAIGRMLTLDDAL
                     ALSVAFRMAGGEGSPLLDCLATQIETEILAERAEACLAQAAGLHAKETGEAVSWAVAA
                     SLPTAGRSVLDRAERACERSVNAGLKMLAAIRARLGRRLKFRKLRGQEVPPPMEA"
BASE COUNT          618 a         1443 c         1468 g          646 t
ORIGIN      
        1 ccccccccgg aacatgctgg gaacgggccg cgcgttcacg acatcgtctc cgccagctcg
       61 cgcgccggcg cttcctcggg ccagtccttc gaatcgtcga tccgcggcgg gcgcggcggc
      121 ggcccgccga attcctggtc cacgatcgcc tccgcctcgg ccatgcgtcg gccgacttcc
      181 gctttcgaca ccttcacgta gacggcgacc gcgtagaact cgtcatcttc ctggagcgcg
      241 gggatcgagg tctggccttc ggtcggccaa tgggttctgc cctcgagcgc ggtaccttcg
      301 aaggccggga cggccaggcg caccgcggcc gcctcgatct ttctgcggcg gatccgcgcg
      361 agtcgctcga cctcgctctc gccgtccttc agcggcagga acgaccccgg gcgacgcgtg
      421 ggaggcgcgc cggcgtcggc ggccggcgcg gcggcgagct taaagccttc gtcagtgagc
      481 tcgacggagg cgccgtccgc ggcgatcgcg cagatcacga aggcggacgc gcgctcgtcg
      541 gggccgaggc ccgccagcga atagacgcgc tgcaggcctt ccggagtctt cggccactgc
      601 catgcaccgc cgccccaggc cttgagtacc gcccctccat agccggcggc ggcggcgacg
      661 cgcagcgcct cgccctcgcc cttctgcgtc gcgagccggt cgagcagccc ggtgtcgagg
      721 atgacctcgc catcctcggc gcccatgaac aggtcgcggt cgagacggcc gccggccgcg
      781 acataggctt cgaccccgac atagcggacc tgccggtccg tggcgcggta gatccggccg
      841 gcgacggcgg agcgaatgcc ggcgacgctg tgcttgccct cccgctcctg ggccgcgaag
      901 atctggcgct ggacctcctg gtcgccgacg ctggcatagg ccttggccga gtccagcgtg
      961 atgcggccgg cgcgaagcgc ctccagggcc tccggtacca ggccggccag gcggaggcgc
     1021 tgctcgacgt aatggacggt cttgccgtaa tgccgggcgc agcgcgccaa cctgacggcg
     1081 ggatcccgct cgccccgtgc ttcatagtcg gcgacgatcg ccgcgtaggc ctcgacctcg
     1141 tcggcggggt tcatggcaac cttgtggacg ttttcggaga ggctgatctc gcggccctcc
     1201 tcgacctccc tgacctgaat agggatggcg ctgggatcga ccttgccggc tgtcgagcgc
     1261 caccgggccg tcttgtcgag cagctgcagg gcgagcagcc tgcggccgcc ggcgatcacc
     1321 ccgaagcggc cacggccctt gccttcgtcg acgacgacga gcggctcgat caggccgtgc
     1381 gcagcaatgt cgtcggcgag cgcgtcgatg tcgagggcac ggtccgtccg gcggacgttg
     1441 aggtccgaaa tatagagctt cgccagcgcc gcagtgctgc tattcatgct ccgtctcctg
     1501 gttaagggca ggggatatgc gagcgccggc ttcagacacc ggcgcttggg gttcggggcc
     1561 ggcttcaggg ccggcctcgg gcttcaccag ctgcgcgggg ccggtgacgc caagcgacac
     1621 cacagggtgc gttcgccccg agatcgccag cacgagcgca ccgccggagg cgagtagctc
     1681 cagctccgcc ggcccaggcc gccataagct ctccatgaaa ggccgaccgg actgctcgac
     1741 atccgcgatt tcaagcgttc cgcactcgcc ctcccggagc tcgctccagt ctatgggcga
     1801 cccgaatcgg cgagtgcggc ggggatgggg gaggggctcc atcactccat ccccagagca
     1861 gctttgtagg cgccgaggag cgcgtcggct tcctggcggt cgtgcttctc catcttccgg
     1921 agccggacga tcgtgcggat cgtcttcgtg tcgtagccgc gcgacttggc ctcggcatag
     1981 acatccttca cgtcgtcggc gaccgccttc ttctcctcct cgccgcgttc gatccgctcg
     2041 atcaggaggc gcagctcgtc ggccgcgatc gtgccctcag ccatgcggaa tctccgcaca
     2101 ggaagggaag gcgatcgcag tcaatgcctg ttcggctgcc gctatggctg cttccagagc
     2161 ctcaacgacc tcctcaacat cctcgtcgag cgaagctctg tcggggcgac cttcggcgtc
     2221 gctaaggcag tggccggaga ggttgcaggc gagctcgttt cgcagagccg gtagcccaag
     2281 ctcgattgcc ttcaccagag aaggcaccgc cgcgatcgcc cggccgttca aattcctgtc
     2341 ggcggcgggc ccgcccgagc cgacactcgc aacgacgtag cccttgttac tctcgttggt
     2401 gacgaggttt cggcgggcgt cgaagaccca acgctgtgcg gccagggggg gcagctcttc
     2461 agccatggat cagctccccc gtgtgctgag gcttggcggc taccggtggg cggccggaac
     2521 cggcgcagac ggtgcagggc tcggccgggt actttcccgg ctcaagcgcc catccgtcgc
     2581 agtgcgggca tttcggggcg ggctcagcca tgctgaagct cctcgatctg gggcggagag
     2641 aagcgggcga tcagcgccgc ctggtcggcc gcgctgaggt tctgcggaag cgtcgtggcc
     2701 ttccggatgg ctagctcgcc gccctcgatc gcgtcctcta ggtcggcgac ctcgtcgacc
     2761 aagtcgaggt ctaaggtcga ccgaagcggg ccagccgccg agtgcaagca gccaatctcg
     2821 aggagccccc cgagctggcg cttcatggcg gtgacggcca cctgcagggc cttgagcagc
     2881 tcggggcccg cccactcggc aagccgcgga tcgtcgagct cggccgccgg ctgtgcggct
     2941 ggcgcggtca tgacggccac gcccaggcga ggagggcccg gccggggact aaagcgaggg
     3001 cgagaaggcc tagcaggatg caggcccggt cgaaggcacg gcgggcccgc gcctcggcca
     3061 gacgagcggg agcggcaaga cgggccaggt tgggatcatg ccgcatcgcg cacctccccg
     3121 ccgacgttga ccggattctg aaggacgtcc ccctcggcgg ggtccggaat gcacatcgtg
     3181 caaagcgggg cttcgtcgac tccgacgcgg gcccagtcgc aaggatcgcc gcattggtca
     3241 tggcacgggt cgaattcgct gcaggcgcag tctcggcaga tcggcgggag caccaccttg
     3301 gcgccggacg cgaaaaagac gagcgccaga tagacgttcg gatcgaaggg gaaggcgccc
     3361 ttcagtggct cgactgagag cgtcatcacc ggggcggtgc cgctctcaag ggcccgcagc
     3421 tgggccatga gctccccgcg agtaatgccg ctgccgtcat cggcaaacca gctgacgact
     3481 tggtcgagcg tctggccggc ggcctcgcgt cgctttcgaa tgtaggtgcc gggggccatt
     3541 gccgcggcga tgggggtctc ggtcatccgg ttggtccttc ttcagacggc agaaaggcgt
     3601 cgccggaagc gggggaatgc cgctctcgac gtcgcgatcg gatggtggat ggaactggcg
     3661 cgaacagcgc cggcggtgcc ggccgacccg gctacgcgac tcaggcctcc atgggcggcg
     3721 gcacctcctg gccgcggagc ttcctgaatt tcagccgcct gccgaggcgg gcccggatgg
     3781 cggcaagcat tttgaggccg gcgttgaccg accgctcgca ggcgcgctcg gctcgatcga
     3841 ggaccgagcg cccggccgtg ggcagcgacg cggcgacggc ccaggagacg gcctcgccgg
     3901 tctccttggc gtgcagaccg gcggcctggg cgaggcaggc ctcggcccgc tcagcgagga
     3961 tctcggtctc gatctgagtc gcaaggcagt ccagaagggg tgagccttcg ccgccggcca
     4021 tccggaaggc gacgctgagc gcgagggcgt catccaaggt aagcatccga ccgatcgccg
     4081 ggccgtaacc aggatcgccc cagctgcgca gagtcgacgg ggacttgcag accagctcgg
     4141 cggcctggtc ccagccaatc cgctcagcca ggcgc
//
LOCUS       DASWNU010000281         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_218983, whole genome shotgun sequence.
ACCESSION   DASWNU010000281 DASWNU010000000
VERSION     DASWNU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218983"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1435)
                     /locus_tag="VGB57_05560"
     CDS             complement(29..1435)
                     /locus_tag="VGB57_05560"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0130853.1"
                     /translation="MRIAAALAVCLLSSTALAADPEPDKWDSKAREIFARSIEISTVP
                     GRGQMPAQARYLADQFKAAGFQESDIRIMPYRARPGDETAALIVRWRAARPSGKKPIL
                     LLAHMDVVEALPADWSADPFKFREEGGYFYGRGAMDNKAGMVAITTALLRLRSEGFGP
                     TRDLIVFFTGDEETEGKGAALGAKEWRKWTDAEFALNSDAGGGAFTKDGRSLGFGIQT
                     AEKTYQSFTFSAKNKGGHSSRPRPDNAIYDLAGALKRLEAHRFTPMLNETTRAYFTER
                     AKMEKGPLGEAMRRWVADPGDGAAADAIEASELEVGTTRTRCVATRLEGGHADNALPQ
                     LARATVNCRIMPGVDPRDVEAELKRVAGPGVEVAVSDPGVPTPASPLRPDVVRAYTDA
                     VRKRHPGAAIIPSMSTGATDGLYFRAAGIPVYGVDGSWGVSPDDERAHGRDERLPVKA
                     LYDDVDHWVDMLKALAGR"
     gene            complement(1609..>1911)
                     /gene="zapE"
                     /locus_tag="VGB57_05565"
     CDS             complement(1609..>1911)
                     /gene="zapE"
                     /locus_tag="VGB57_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HEY0130854.1"
                     /translation="RLCGEARGAADYLAIARRYHSVILVGIPVMGPDRRNEAARFVTL
                     IDAFYEHKVKLLAAADAPPDGLYPAGDGAFEFQRTASRLIEMQSRDYLAAGHGEGG"
BASE COUNT          281 a          671 c          665 g          294 t
ORIGIN      
        1 attccggcct tcgccggaat gacgggccct atctccccgc aagcgccttc agcatgtcca
       61 cccaatggtc gacatcgtcg tacagcgcct tgaccggcag ccgttcgtcg cggccatggg
      121 cgcgctcgtc gtcgggggag actccccagc tgccgtcgac cccgtagacc gggatcccgg
      181 cggcccggaa atagaggccg tcggtggcgc cggtcgacat gctcgggatg atcgccgcgc
      241 cgggatggcg cttccggacc gcgtcggtat aggcgcggac cacgtcgggc cgaagcggcg
      301 aagccggcgt cggaacaccc gggtcgctca ccgccacctc cacgcccggg cccgcgacgc
      361 gcttgagctc ggcctcgacg tccctggggt cgacccccgg catgatccgg cagttgaccg
      421 tggcgcgggc cagctgcggc agggcgttgt cggcatggcc gccctccagc cgggtggcga
      481 cgcagcgggt ccgggtcgtg cccacttcga gctcgctcgc ctcgatcgcg tcggcggccg
      541 cgccgtcccc gggatcggcc acccagcgcc gcatggcctc gcccagcggc cccttctcca
      601 tcttcgcccg ctcggtgaaa taggcccggg tcgtctcgtt cagcatcggc gtgaaacgat
      661 gcgcctcgag ccgcttgagc gctcccgcga ggtcgtaaat ggcgttgtcg ggtcgcggcc
      721 gcgagctgtg gccgcccttg ttcttggccg agaaggtgaa gctctgatag gtcttctccg
      781 ccgtctggat tccgaagccg agcgagcgcc cgtccttggt gaaggcgcct ccgccggcgt
      841 ccgagttgag cgcgaactcg gcatcggtcc acttccgcca ttccttcgcc ccgagcgcgg
      901 cgcccttccc ttccgtctcc tcgtcgccgg tgaagaagac gatcaaatcc cgggtgggcc
      961 cgaacccttc cgagcgcagc cgaagcagag cggtggtgat cgccaccatc ccggctttgt
     1021 tgtccatggc gccgcggccg tagaaatagc cgccctcctc ccggaacttg aacggatcgg
     1081 cggaccagtc ggcgggcagc gcctcgacca cgtccatatg ggcgagcagc aggatcggct
     1141 tcttccccga aggcctggcc gcccgccagc gcacgatcag agccgccgtc tcgtctcccg
     1201 gccgggcccg atagggcatg atcctgatat cgctctcctg aaagccggcg gccttgaact
     1261 gatcggcgag gtagcgcgcc tgcgccggca tctgcccgcg tcccggcacg gtggagatct
     1321 cgatcgagcg ggcgaagatc tcgcgggcct tcgaatccca tttgtcgggc tcgggatctg
     1381 cggcgagagc ggtggaggag aggagacaga cggcaagggc ggcggcaatg cgcatgaagg
     1441 agggctccgc tacatgaggc ggacggaagc tggaccggcg ggacggcagc gtcaacggtc
     1501 agaacgcgtc gccccagcga aggctggggc ctcggggggt gatggacgcg gcgcccttca
     1561 cccgcctgga ccccagcctg cgctggggtg acgggaagga ggttacggct acccgccctc
     1621 cccgtgcccc gccgcgaggt aatcccgaga ctgcatctcg atcagccgcg acgccgtgcg
     1681 ctggaattcg aaggcgccgt caccggcagg gtacagcccg tcgggcggag cgtcggcggc
     1741 ggcgagaagc ttcaccttat gctcgtagaa ggcgtcgatc agggtgacga agcgcgccgc
     1801 ctcgttgcgc ctgtccgggc ccatgaccgg aatgccgacg aggatgacgc tgtgatagcg
     1861 ccgggcgatg gcgagatagt cggcggcgcc gcgggcctcc ccgcacagcc g
//
LOCUS       DASWNU010000282         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_479515, whole genome shotgun sequence.
ACCESSION   DASWNU010000282 DASWNU010000000
VERSION     DASWNU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479515"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGB57_05570"
     CDS             <1..282
                     /locus_tag="VGB57_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0130855.1"
                     /translation="MGAEEAERSGLVARVVPADRLIEEALATAEKIAAMAPLAAIATK
                     EMVNAAFETGLAQGIVFERRLFHGLFGTEDQKEGMAAFVDKRQAQWTGK"
     gene            405..1307
                     /gene="mmsB"
                     /locus_tag="VGB57_05575"
     CDS             405..1307
                     /gene="mmsB"
                     /locus_tag="VGB57_05575"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEY0130856.1"
                     /translation="MARIAFIGLGNMGGGMASNLVKAGHEVRAFDLSEAALQKAEANG
                     CARAATAAEAVADAEAVVTMLPAGKHVRDVYESSVIGTAPTSALLIDCSTIDVATARE
                     EIEKAAAAGYAMVDAPVSGGIAAADAGTLTFMVGGTGEAFERAREILEKMGKAVIHAG
                     GPGSGQAAKICNNMLLGASMVATCETFVLAGKLGLDPQTFFDIASKASGQCWSMTTYC
                     PVPGVGPVTPADRDYEGGFAAALMLKDLRLAMEAAQSVDSYTPMGSHAEELYTRFAER
                     LGGGSKDFSGIIKMIDDSWKTTES"
     gene            complement(1408..2601)
                     /gene="wecB"
                     /locus_tag="VGB57_05580"
     CDS             complement(1408..2601)
                     /gene="wecB"
                     /locus_tag="VGB57_05580"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY0130857.1"
                     /translation="MVQRIHLIAAARPNFMKVAPLFHALAAAPGFTPILIHTGQHYDV
                     NMSDAFFADLRLPEPDHHLGVGSGSHAEQTGGVMIAYEKVALKDRPDWIVVVGDVNST
                     AACAMVAGKLGIPVVHLEAGLRSRDRTMPEELNRLVTDVLADVLWTPSPDGDENLLAE
                     GIPAGRITRVGNIMLDSFELVRPAIEAADVPGELGLERGGYGVVTLHRPSNVDDPGQL
                     IRLVDSLIEVQRRLPLVFPVHPRTAKRLAGALFDSRLARAGVKLIEPLPYVRFMSLVT
                     GAAAAITDSGGIQEETTYLGIPCLTLRENTERPVTITEGTNRLVKPETLLLELERALT
                     GPRPVRCPELWDGKAAARCVEDLRRRSAVAKLPIAKPSPAELAARYGGGERRKAGGSD
                     YRRAT"
BASE COUNT          454 a          904 c          948 g          413 t
ORIGIN      
        1 atgggcgccg aggaagcgga gcgctccggc ctcgtcgcca gggtcgttcc ggcggaccgg
       61 ctgatcgagg aagctctggc gacggcggag aagatcgccg ccatggcccc gctcgccgcc
      121 atcgcgacga aggagatggt caacgccgcc ttcgagaccg gcctggcgca aggcatcgtc
      181 ttcgagcgcc gcctgttcca cggactgttc ggaaccgagg accagaagga aggcatggcg
      241 gcgttcgtcg acaagcgtca ggcgcagtgg accgggaagt gacgtcgccc ctcccccgtc
      301 attgcgagcg tagcgaagca atccagtgcg gcgcgtcagc tgatggattg cttcgtcgct
      361 acgctcctcg caatgacggg ccaaataatc caccggagta gaaaatggca cgaatcgcat
      421 tcatcgggct cggcaacatg ggcggcggga tggcctcgaa cctggtcaag gcggggcatg
      481 aagtgcgcgc cttcgacctc agcgaggcgg cgctgcagaa ggcggaggcc aatggctgcg
      541 cccgggccgc cacggcggcc gaggcggtcg ccgatgcgga ggcggtggtg accatgcttc
      601 cggccggcaa gcacgtccgc gacgtctacg aatcgagcgt gatcggcacc gcgcccacaa
      661 gcgccctcct gatcgactgc tcgaccatcg acgtcgccac cgcccgcgag gagatcgaga
      721 aggcggccgc ggccggctat gcgatggtcg acgcgccggt ctcggggggg atcgcggcgg
      781 ccgatgccgg caccctcacc ttcatggtcg gagggaccgg cgaggcgttc gagcgcgcgc
      841 gggagatcct ggagaagatg ggcaaggcgg tgatccacgc cggcggccca ggctcgggcc
      901 aggcggcgaa gatctgcaac aacatgcttc tcggcgcctc gatggtcgcg acctgcgaga
      961 ccttcgtcct tgccggcaag ctcggcctcg atccccagac cttcttcgac atcgcctcca
     1021 aggcctccgg ccaatgctgg tcgatgacca cttactgccc cgttcccgga gtcggcccgg
     1081 tcacccccgc cgaccgcgac tatgaaggcg gtttcgcggc cgctttgatg ctgaaggacc
     1141 tgcggctggc gatggaggcg gcgcagagcg tcgactccta cacgccgatg ggctcgcatg
     1201 cggaggagct ctacacccgc ttcgccgagc ggctgggcgg aggctcgaag gacttttccg
     1261 ggatcatcaa gatgatcgac gacagctgga agactacgga gtcgtaatcc gtcattccgg
     1321 cgaaggccgg aatccaggga catcgacgtt cgcggctaca ctcctcgctc cgtgttcatg
     1381 gattcccgcc ttcgccggaa tgacggttca agtcgcccga cggtaatccg atccgcccgc
     1441 ctttctccgc tcgccgccgc cgtagcgggc ggcaagctcg gccgggctcg gcttcgcaat
     1501 cggcagcttc gctactgccg accgccggcg caggtcctcg acgcagcgcg ccgcggcctt
     1561 gccgtcccaa agctccgggc agcgcaccgg cctcggcccg gtcaaagcgc gctcgagctc
     1621 gagcagcagg gtctcgggct tcaccagccg gttcgtccct tcggtgatcg tcaccggccg
     1681 ctcggtattc tccctcaggg tcaggcaggg aatcccgaga taggtggtct cttcctggat
     1741 tccgcccgaa tcggtgatcg ccgccgccgc cccggtcacc aggctcatga agcggacgta
     1801 gggcaaaggc tcgatcagct tgaccccggc gcgggcgagg cggctgtcga acagggcccc
     1861 cgcgagccgc ttcgccgtgc gcgggtggac cgggaagacg agcggaagcc gccgctggac
     1921 ctcgatcagc gaatcgacca gccggatgag ctggcccgga tcgtcgacgt tggaaggacg
     1981 gtggagggtc accactccgt agcctccccg ctccagcccg agctctccag gcacgtccgc
     2041 cgcctcgatc gccggccgga ccagctcgaa gctgtcgagc atgatgttgc cgacccgggt
     2101 gatccggccg gcggggatgc cctccgcgag gaggttctcg tcgccgtcgg gcgaaggggt
     2161 ccagagcacg tcggcgagca cgtcggtgac gagccggttg agctcctccg gcatggtccg
     2221 gtcgcggctc ctgaggccgg cctcgagatg gacgaccgga atcccgagct tgcccgcgac
     2281 catcgcgcag gcggcggtcg agttgacgtc gccgacgacg acgatccagt cgggtcgatc
     2341 cttcagagcc accttctcat aggcgatcat cactccgccg gtctgctcgg catggcttcc
     2401 cgagcccact ccgagatgat gatcgggctc cgggagcctc agatccgcga agaaggcgtc
     2461 ggacatgttg acgtcgtaat gctggccggt gtggatgagg atcggcgtga agccgggcgc
     2521 cgcggcgagg gcgtggaaaa gcggcgccac cttcatgaaa ttcgggcgcg cggcggcgat
     2581 caggtggatg cgctggacca tgaggcggag gtagcgggaa agggtaaaga tgtggttggc
     2641 ccgtcgcccc agcgaaggcc ggggccccgg cgggtgaaga ccgcggcgcc tttcacccgc
     2701 cgcgcagcgg aaaaagagg
//
LOCUS       DASWNU010000283         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_193833, whole genome shotgun sequence.
ACCESSION   DASWNU010000283 DASWNU010000000
VERSION     DASWNU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193833"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="phhA"
                     /locus_tag="VGB57_05585"
     CDS             <1..724
                     /gene="phhA"
                     /locus_tag="VGB57_05585"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEY0130858.1"
                     /translation="TIPQNWERFGAEEHRVWDILYARQRRKLTGRAVATFEEGLDSLL
                     LSRSGIPDFDDLNERLFPRSGWTVVPVPGLLPDEIFFAHLARRQFPAGNFIRPASSLD
                     YLEEPDVFHDVFGHVPMLATRWFADFMQRLGEEGLKAVAKNRVAIFSRLYWFTVEFGV
                     AWEEGALKLYGAGLASSFGEAAYALDSARPERLSFDLERVIRTPYRSDVFQPRYFVVD
                     GLDTLRTGIDLDRLEESLDALL"
     gene            829..1641
                     /locus_tag="VGB57_05590"
     CDS             829..1641
                     /locus_tag="VGB57_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0130859.1"
                     /translation="MPFSSTVAAAARTVLLAAEPKAKVRMARAAARAWRRGELDHAFD
                     VAMPDLPARPDRPELLPPSRMPKRGRGGSERGRIAMLHALAHIEFSAIDLAFDLAGRF
                     GADYPPAFIDDWFSVGADEAMHFALLDRRLRTLGSAYGDLPAHDGLWEAAAETAGDPL
                     ARLAVVPMVLEARGLDVTPAAVARFEAAGDMRSAAILGRIYRDEIRHVAAGSKWFRWG
                     CESRRIAPVATWKRLVGAHFRGVLKPPFNDSARDEAGLSRDYYAALAVAGDV"
     gene            1814..2719
                     /locus_tag="VGB57_05595"
     CDS             1814..2719
                     /locus_tag="VGB57_05595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0130860.1"
                     /translation="MFRTMFKPRDVFLHDGKALRRVRVGSGVQAAAAFAALFLLISSL
                     FAAAQLAGAASAPSNADVARMERELAAMQADVAVIKEAAQRRYQLTAGEVRRLGLDPS
                     RLHHTGVGGPFEAVETVRAADPNFKALFNSWTRLDQLEQGTVAIPSTEPVRGTNFTSG
                     YGVRSDPFRGRAAMHAGIDLAGPIGTPIYATADATVGRSEYNSGGYGNLVELEHGHGI
                     QTRYGHLSKSLVSAGQRVKRGDMIGLMGSTGRSTGSHLHYEVRIDGKAVNPVPFLQST
                     SYLQSVQQRAAAATRIAIGGPAAAN"
     gene            2906..3799
                     /locus_tag="VGB57_05600"
     CDS             2906..3799
                     /locus_tag="VGB57_05600"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0130861.1"
                     /translation="MQRRCRYLLAGCVLALASAAIASETVTYSYDALGRLTAMTSSGS
                     ANNGVTTGIAYDPAGNRSAYNVSGVVTPPPEPPEPPVTPAPPPPPAFAVSGGTVNEGG
                     NLAFTVSRSNLTAGTFSVSYATSNGSAAQYSDYYPASGTLTFTTGASQTVSVLTVKDN
                     VAEPAETMLMTLSNPTGGATIQAGAGQATGTINASAANAPPTAVADNFSVARCTAGSF
                     NVVANDTDPEGDYPLVLVAVSGQAAQQGASVGSSTNVAWAKARYAGGYYMGYTVADSR
                     NASSSGTLKITVTGSGSTACP"
     gene            3841..>5606
                     /locus_tag="VGB57_05605"
     CDS             3841..>5606
                     /locus_tag="VGB57_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130862.1"
                     /translation="MHDIRRMLRSGPPALARFLCHALAGVLILLGGTILLAGGIRSAH
                     AQGLNDLVPRQEFRDANGVDMDGAIYRHHEALLRIGNGPHPLALNLHISSPGPEAAGV
                     CPASLSGDVGNAIYHALDSHAYFGNTAIGTALFTVVLPHQSAKFAIYGSGFNRTIVPS
                     DAAYVTAAFDATNVYLDYVGGDGAEATFSQLHTPLRAFQDQSLSHTADRVRYPDGETW
                     IYRYNDVQYAAPGCPSIKVSRVRSIVSSRGYGLQFNYAADAGGTVTSSSVVRDFVSPI
                     RVAAYNKASVFCDESALLSCASVNALSTAVAFAYDRTNRKVTVSKANGEQVELAFSLG
                     ATLNLTGVTRPGGASRTMTYREWEEQEGPTYRTLENLTEAGRTWSYGWGPMGAEAVTT
                     VIEPGNATTAYHFIHGMPSMILDPLGRETLVGYDDYSRLLRRRAPEGNELTASYDVRG
                     NLNLLKEIPKPGSGLPTLQSSAFFPPGCTAADRRRCNRPTSVTDRRSNTTDYTYSPDH
                     GGVLTETGPAVPSRQADGSLASVRPQKRYEYAQRQAWVSNGTGGYVQAAPVWLLVRER
                     YCRITSAIGPSCAGGAADEVVT"
BASE COUNT          903 a         1999 c         1791 g          913 t
ORIGIN      
        1 gaccatcccg cagaactggg agcggttcgg cgccgaggag catcgggtct gggacattct
       61 ctatgcccgg cagcgccgca agctcaccgg ccgcgccgta gcgacgttcg aggaagggct
      121 cgactccctg ctcctgtcgc gttcgggaat tcccgacttc gacgatctga acgagcggct
      181 gttcccgcgc tcgggatgga cggtcgtgcc ggtgccgggc ctgcttccgg acgaaatctt
      241 cttcgcccat ctcgcccgcc gccaattccc cgcgggcaac ttcatccgcc ccgcctccag
      301 cctcgactat ctggaggagc ccgacgtctt ccacgacgtg ttcgggcatg tgccgatgct
      361 cgcgacacgc tggttcgcgg acttcatgca gcggctcggc gaggaagggc ttaaggcggt
      421 ggcgaagaac agggtcgcga tcttctcgcg cctctactgg ttcacggtcg aattcggagt
      481 ggcctgggag gagggcgcgc tcaagctcta cggggccggc ctcgcctcga gcttcggcga
      541 agcggcctat gcgctcgaca gcgccaggcc ggagcggctt tccttcgacc tcgagcgcgt
      601 gatccggacg ccctaccgct ccgacgtctt ccagccccgc tatttcgtgg tggacgggct
      661 cgacacgctc cggaccggga tcgatctcga ccggctggag gaaagcctcg acgcgctgct
      721 ctaggctccg tcattgcgag cgaagcgaag caatccagtg cggcttcaga ggcccgctgg
      781 attgccgcgt cgcttcgctc ctcgcaatga cgggcttata gcgaggccat gcctttttcc
      841 tcaaccgtcg ccgccgccgc ccgaaccgtc ctcctcgccg ccgagccgaa ggccaaggtg
      901 cgcatggctc gcgccgccgc ccgggcctgg cggcggggcg agctcgacca tgcgttcgac
      961 gtcgcgatgc cggacttgcc ggcccggccc gatcggccgg agctgctgcc gccgtcgcgg
     1021 atgcccaagc gagggcgcgg cggctccgag cgggggcgga tcgccatgct ccacgcgctc
     1081 gcccatatcg aattttccgc gatcgacctc gccttcgacc tcgccggccg cttcggggcg
     1141 gactacccgc cggcctttat cgacgactgg ttctcggtcg gggccgacga ggcgatgcat
     1201 ttcgccttgc tcgaccggcg ccttcgcacg ctcggctcgg cttacggcga cctgcccgcc
     1261 catgacggcc tgtgggaagc cgcggcggaa acggccggcg atccgctcgc ccgcctggcg
     1321 gtcgttccga tggtccttga agcgcgcggg ctcgacgtca ctccggcggc ggtggcccgg
     1381 ttcgaggcgg ccggcgacat gcgttcggcc gccatcctcg gccgcatcta ccgcgacgag
     1441 atcaggcatg tcgcggcggg ctcgaaatgg ttccgatggg gctgtgaatc gcggagaatc
     1501 gcgcctgttg cgacctggaa acgattggtc ggcgcccact tcagaggcgt tctgaagcct
     1561 ccattcaacg attcagcgcg cgacgaagcc ggtctgtccc gcgattacta cgccgccctt
     1621 gccgtggcag gagatgttta acagtctcag ctcacacgtc gcggaggggg caactccctg
     1681 acgtatcgag ggggcggaaa cgccttgaat ttaatgcttt cggtcgcgtt tgcgcgtggc
     1741 cgcgcccagt aaggggcggc cagtcgggga attgatggtg ctgactaaaa caaacaatgc
     1801 ggggctcttc ggaatgttcc gtacgatgtt caagccgcgc gatgtttttc tccatgacgg
     1861 caaagccttg cgccgggtcc gggtcggttc cggggtccag gccgccgccg ccttcgccgc
     1921 tctcttcctg ctcatctctt cgctcttcgc cgccgcccag ctcgccggcg ccgccagcgc
     1981 cccttccaac gccgacgtcg cccggatgga gcgcgagctc gccgcgatgc aggccgacgt
     2041 cgccgtgatc aaggaagcgg cccagcgccg ctaccagctc accgccggcg aagtccgccg
     2101 tctcggcctc gacccgagcc ggctccacca caccggcgtc ggcggtccgt tcgaggcggt
     2161 cgagacggtc cgcgccgccg accccaattt caaggcgctg ttcaacagct ggaccaggct
     2221 cgaccagctc gagcagggca ccgtcgccat cccctcgacc gagccggtcc ggggcaccaa
     2281 cttcacctcc ggctacggag tccgctccga ccccttccgc ggccgcgccg cgatgcacgc
     2341 cgggatcgac ctcgccggcc cgatcggaac cccgatctac gccaccgccg acgccaccgt
     2401 cggccgctcc gaatacaaca gcggcggcta cggcaacctc gtcgagctcg agcacggcca
     2461 cggcatccag acccgctacg gccatctttc caagtcgctc gtctcggccg gccagcgcgt
     2521 caagcgcggc gacatgatcg gcctgatggg ctcgaccggc cgctcgaccg gaagccacct
     2581 ccactacgaa gtccggatcg acggcaaggc ggtcaacccg gtccccttcc tccagtcgac
     2641 ctcctatctc cagtcggtcc agcagcgcgc cgccgccgcc acccggatcg ccatcggcgg
     2701 ccccgccgcg gcgaattgat ccaagcttag gagagagccg aaaaggcccg agccccgccg
     2761 gagacggcgg ggcatttgcg ttcgggcgta cgcggtcggg tccgattcgg accgaacggg
     2821 atttgcaatg taggatattc tatgttccac tgatgttctg gtgagcgggg gaggaatccg
     2881 agtcgccggc gttccggggg gcctgatgca gcgacgttgc cgttacctgc tggcgggctg
     2941 cgtcctcgcg ctggcgagcg cggccatcgc gagcgagacc gtcacctaca gctatgacgc
     3001 gctcggacgg ctcaccgcca tgacgagcag cgggagcgcc aacaatgggg tcacgaccgg
     3061 catcgcctac gacccggcgg ggaaccgttc agcctacaac gtgtccggag tcgtcacgcc
     3121 gccgccggag ccgccggagc cgccggtgac tccggccccg ccgccccccc cggccttcgc
     3181 ggtgagcggc gggaccgtga acgaaggcgg caacctcgcc ttcaccgtct ccaggtccaa
     3241 cctgacggcc gggacgttca gcgtcagcta cgccacctcg aacggcagcg cggcccaata
     3301 ttccgattat tatccggcga gcggcaccct cacgttcacg accggagctt cgcagacggt
     3361 gagcgtcctc accgtcaagg acaatgtcgc cgagccggcc gagacaatgc tgatgaccct
     3421 gtccaacccg accggcggcg ccacgatcca ggccggcgcc ggccaggcaa cggggacgat
     3481 caatgcgagc gccgccaacg ccccgccgac ggccgtagcc gacaatttct cggtcgcgcg
     3541 ctgcaccgcc ggaagcttca acgtcgtcgc caacgacacc gatccggaag gcgattaccc
     3601 gctggtcctg gtcgccgtca gcggacaggc ggcccagcaa ggcgcgtcgg tcggcagctc
     3661 gaccaacgtc gcctgggcga aggcccgtta cgccggcggt tattatatgg gctatacggt
     3721 cgcggacagc cgcaacgcga gctcgtccgg caccctcaag atcactgtca ccggatcggg
     3781 gagcacggca tgtccctgag cgattccatt cactgcccaa gtcggcccgg gaggctgctg
     3841 atgcatgaca tccgaaggat gctgcgctcc ggaccgccgg ccctggcccg gttcctttgc
     3901 cacgcgctcg ccggcgtcct gatcctgctt ggcgggacga tcctcctggc cggcggcatc
     3961 cggtcggcgc acgcgcaagg gctcaacgac ctggtgccgc gacaggagtt cagggacgca
     4021 aacggggtcg acatggacgg cgcgatttat cgtcatcacg aagccctgct tcggatcggc
     4081 aacggcccgc accctctcgc gctcaacctg cacatttcca gtccggggcc ggaagcggcg
     4141 ggggtgtgcc ccgcgagcct ctccggagat gtcggtaacg ccatttacca cgcgctcgac
     4201 tcccatgcct atttcggcaa caccgccatc ggcacggcct tgttcacagt ggtgctcccc
     4261 caccagagcg ccaaattcgc aatctacgga tcgggcttca accgaacgat cgttccaagc
     4321 gacgccgcct acgtcaccgc cgcgttcgat gcgaccaacg tctatctcga ttatgtggga
     4381 ggcgacggcg ccgaagcgac cttttcccaa ttgcacacgc cgctgcgcgc cttccaggac
     4441 caatccctgt cgcatacggc ggatcgggtt cgctatcccg acggagagac gtggatctat
     4501 cgctataacg acgtgcaata tgccgcgccg ggctgccctt cgatcaaggt ctcgcgggtc
     4561 cgctcgatcg tctccagccg cggctacggg ctgcagttca actatgccgc cgacgccggc
     4621 ggaacggtga cctcgtccag cgtcgtccgc gacttcgtct cgccaatacg ggtcgccgcc
     4681 tacaacaagg cgtccgtctt ttgcgacgag tcggcgctgc tgagctgcgc gtccgtcaac
     4741 gcgctgagca ccgccgtggc cttcgcctac gaccggacga accggaaggt gacggtgagc
     4801 aaggccaatg gcgagcaggt cgagctcgcc ttcagcctcg gggcgacgct caatcttacc
     4861 ggcgtcaccc ggcccggcgg tgcctcccgg acgatgacct atcgggaatg ggaggagcag
     4921 gaaggaccga cttatcgcac cctggagaac cttacggagg cggggcgtac ctggtcctat
     4981 ggctggggtc ccatgggcgc ggaggcggtg acgacggtga tcgagccggg caacgccacc
     5041 accgcttatc attttatcca cggaatgccc tccatgatcc tcgatcctct cgggcgggag
     5101 accttggtcg gctatgatga ctattcgcgc ctgcttcgcc gccgagcgcc ggaaggcaat
     5161 gagctgaccg cctcctacga tgtccgcggc aacctcaatc tgctgaagga aattcccaag
     5221 ccgggctccg gcctgccgac gctgcagtcg agcgcgttct tcccgccagg ctgcaccgcc
     5281 gcggaccggc gccgctgcaa tcggccgaca tcggtgaccg ataggaggag caacacgacc
     5341 gactacacct acagccccga tcatggcggc gtgctgacgg agacgggacc ggcggtgccg
     5401 agccgccagg ccgacggcag cctcgcgagc gtgcgtcccc agaaacggta tgaatatgcc
     5461 cagcgccaag cctgggtctc gaacggcacc ggcggctacg tccaggccgc gcccgtctgg
     5521 ctgctggttc gggagcgata ctgccggatc acctctgcaa tcgggccgag ctgcgccggc
     5581 ggcgccgcgg acgaggtcgt caccga
//
LOCUS       DASWNU010000284         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_62024, whole genome shotgun sequence.
ACCESSION   DASWNU010000284 DASWNU010000000
VERSION     DASWNU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62024"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /gene="mutL"
                     /locus_tag="VGB57_05610"
     CDS             complement(<1..1558)
                     /gene="mutL"
                     /locus_tag="VGB57_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY0130863.1"
                     /translation="MSIRRLPEHLVNRIAAGEVVERPASALKELVENAIDAGARRIAI
                     SLSGGGIERIEVTDDGCGMTPADMALALERHATSKLPDDAIENVSTLGFRGEALPSIA
                     SVSRISIESRVRGADGWQRVIDNGRLEAEGPAALPPGTRIRVEELFARVPARRKFLRS
                     ERSEYAACADVVKRLAMARPDIAFSLEHDGRRSFSVQAEEDRPARVAALTDRGLAENS
                     IGVDYERGSARLGGVAGLPTFNRGVADHQFLFVNGRPVKDRLLIGAVRAAYQDLLARD
                     RHPILALFIDLPGEEVDVNVHPAKTEVRFRDPAGIRGLIVGGLRSALDGAGHRSAQRP
                     SATALGNWQPAPPSRPANTGFTIPAAPQGSVWDSHQAYAPPPQARAEAADAAPPEAKA
                     FPLGVARGQVAATYIVSEAEDGLVLVDQHAAHERLVLERMRKAMSNGGVARQAMLLPE
                     VVELDESACDRLETRSEELAEMGLELERFGASAMLVRATPALLGHSNIRGLVTDLADE
                     LSAFDEALSLK"
BASE COUNT          276 a          578 c          578 g          288 t
ORIGIN      
        1 ctttgaggct gagcgcctcg tcgaacgctg aaagttcgtc ggcaagatcg gtgaccaaac
       61 ccctgatatt gctatgtccg agcaaggcgg gcgtggcccg gacgagcatc gcgctggcac
      121 cgaagcgctc cagctccagt cccatctccg ccagctcttc ggaacgcgtc tcaagccggt
      181 cgcaggcgct ttcgtcgagc tccacgacct ccgggagcag catggcctgg cgcgccacgc
      241 cgccgttcga catcgctttt cgcatccgct ccagcacgag gcgctcatga gcggcatgct
      301 ggtcgacgag caccagaccg tcctcggcct cggatacgat gtaagtcgcc gcaacctgac
      361 cgcgggcgac gccgagcgga aaggctttcg cctcgggggg cgccgcgtcc gcggcttccg
      421 cccgagcttg gggcggcggt gcgtaagcct gatggctgtc ccagacgctg ccctgaggcg
      481 ccgccgggat cgtgaagccg gtattggcgg gtcgtgaggg cggtgccggc tgccaattgc
      541 ccaatgcggt ggcggaagga cgctgagcgc tgcgatgtcc ggcaccatcg agggccgagc
      601 gcagcccgcc gacgatcagg ccgcgaattc ccgccgggtc gcggaagcgc acctccgtct
      661 tggcgggatg gacattgacg tcgacctctt cgccggggag atcgatgaac aaggccagga
      721 tcgggtggcg gtcgcgggcg agaagatcct gataggcggc gcggacggcg ccgatcagga
      781 ggcggtcctt caccggccgc ccgttgacga aaaggaactg gtgatccgcc acgccgcggt
      841 tgaaggtcgg aagccctgcc acgccgccga ggcgggccga gccgcgctca tagtcgaccc
      901 caatgctgtt ttcggcgagg cctcggtcgg tgagcgccgc cacccgcgcg ggccggtctt
      961 cctccgcctg aaccgagaag cttcggcgac cgtcatgctc gagcgagaag gcaatgtcgg
     1021 gccgcgccat ggcgagccgc ttcacgacat cggcgcaggc ggcatattcc gaccgctcgg
     1081 agcgcaggaa cttgcgccgc gccgggaccc gggcgaacaa ttcctcgacg cggatgcggg
     1141 tacccggcgg cagggccgcc ggcccttctg cttccagccg gccgttgtcg atcacccgct
     1201 gccagccgtc ggcgcccctc acccggctct cgatgctgat cctggagacg ctggcgatgg
     1261 acggcagagc ctcgccgcgg aaaccgagcg tcgagacatt ctcgatggcg tcgtcgggca
     1321 gcttcgaggt ggcatgccgc tccagcgcca gcgccatatc ggcgggggtc atgccgcagc
     1381 cgtcgtcggt cacctcgatc cgttcgattc cgccgccgga aagcgagatc gcgatccgcc
     1441 gggcaccggc gtcgatcgcg ttttccacca attccttcaa cgcgctggcc ggtctttcga
     1501 caacttcgcc ggcggcgatc ctgttaacga gatgttcggg gagtcgcctt attgacatgg
     1561 tctatcctgt agaccaattc gcgcgggcct gaacacatta accttattct ggacgcgccc
     1621 ggcgaccgca ccctatagga tggaagctcg ggcggtgccg gcccttggcg gcgctaagtt
     1681 gttgatgaag cgagacccca cgaaatgtcg tttttccgtt
//
LOCUS       DASWNU010000285         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_373829, whole genome shotgun sequence.
ACCESSION   DASWNU010000285 DASWNU010000000
VERSION     DASWNU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373829"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1796
                     /gene="rtcR"
                     /locus_tag="VGB57_05615"
     CDS             177..1796
                     /gene="rtcR"
                     /locus_tag="VGB57_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HEY0130864.1"
                     /translation="MRPTVVLGFVGSTLDSSKMGPSRWNKWRPSVGLCMHEDLRVDRF
                     ELIHGGLHEGLARYVTSDIAGVSPETKVRPHRLDFADPWDFEEVYGKLLDFARAYAFD
                     PEVEDYLVHITTGTHVAQICLFLLTEARYLPGRLLQSHPSRGTISPAGSWSVIDLDLS
                     RYDSIATRFAMAAAEGSSFLKSGIETRNPAFNRMIDEIEQVALRSRAPFLLMGPTGAG
                     KSQLARRIFDLKRLKHQVSGEFVEVNCATLKGDSAMSALFGHKKGAYTGAGADRPGLL
                     RTADKGVLFLDEIGELGLDEQAMILRAIEEKRFLPVGADREASSDFQLIAGTNCDLGV
                     AVAAGRFREDLFARLNLWTFELPALRDRLEDIEPNLDYELDRFAEREGTRVSFNKEAR
                     RAFLAFATAPEALWAGNFRDLAASVTRMATFAPAGRIDIATVEAEKARLKRFWSNGGA
                     ASDALDQVVDPARLEEIDPFDRVQLAEVIRICRRSRSLSEAGRSLFSASRSRRSSAND
                     ADRLRKYLQRYGLDWAGLQEILRHSGEGRNP"
BASE COUNT          335 a          634 c          628 g          334 t
ORIGIN      
        1 gacgtcgccc ggcgcagtca tggttgcaga gggcgtgcca aaccggattt gccctggaag
       61 aaaactcgca tgtcgctggc aaacagcgac ttttttccca aaggtaaaat cggcgaaatc
      121 gaggcatccg aagatttgct atcgacagag ataaggcttt atcccatggt ataagaatga
      181 gacccactgt cgtcctcggc ttcgtcggct ccaccctcga ctcaagcaag atgggcccgt
      241 cgcgctggaa caagtggcgg ccgagcgtcg ggctgtgcat gcacgaggat ctcagggtcg
      301 accgcttcga gctgatccat ggcggcctcc acgaggggct ggcgcgctac gtcacctccg
      361 acatcgccgg ggtgtcgccg gagacgaagg tccggccgca tcggctcgac ttcgccgatc
      421 cctgggattt cgaggaggtg tacggcaagc tgctcgattt cgcccgcgcc tatgcgttcg
      481 acccggaggt cgaggattat ctcgtccaca tcaccaccgg cacccacgtc gcccagatct
      541 gcctgttcct cctgaccgag gcccgctacc tgcccggccg gctgctccag tcccacccgt
      601 cacgcggcac gatcagcccg gcgggcagct ggagcgtcat cgatctcgac ctgtcccgct
      661 acgacagcat cgccacccgc ttcgcgatgg cggcggcgga agggagctcg ttcctcaaat
      721 cggggatcga gacccgcaat ccggccttca accggatgat cgacgagatc gagcaggtcg
      781 ccctccgttc ccgggcgcct ttcctgctga tggggccgac cggcgccggc aagtcgcagc
      841 tggcgcggcg gatcttcgat ctcaagcgcc tcaagcacca ggtgtcgggc gagttcgtcg
      901 aggtgaattg cgcgacgctg aagggcgaca gcgcgatgtc ggcgctcttc ggccacaaga
      961 aaggcgccta taccggcgcc ggggccgatc gcccgggcct tctccgaacc gctgacaagg
     1021 gtgtcctctt cctcgacgag atcggcgagc tcggcctcga cgagcaggcg atgatccttc
     1081 gggcgatcga ggagaagcgc ttccttccgg tcggggcgga ccgcgaggcg tcgtccgact
     1141 tccagctcat cgcgggcacc aattgcgacc tcggggtcgc ggtcgccgcc ggccgcttcc
     1201 gcgaagatct gttcgcgcgc ctcaatctgt ggacgttcga gctgcccgcc cttcgcgacc
     1261 ggctcgagga tatcgagccg aacctggact acgagctgga caggttcgcc gagcgcgagg
     1321 ggacaagggt cagcttcaac aaggaggcgc ggcgggcgtt cctcgccttc gcgaccgcgc
     1381 ccgaggcgct ctgggcgggc aatttccgcg acctcgccgc cagcgtcacc cggatggcga
     1441 ccttcgctcc ggcggggcgg atcgacatcg ccacggtcga agccgagaag gctcggctga
     1501 agcgcttctg gtcgaacggc ggggccgcgt ccgacgcgct cgatcaggtc gtcgatccgg
     1561 ccaggcttga ggagatcgat ccgttcgacc gggtccagct cgccgaggtg atccggatct
     1621 gccgtcgaag ccgatcgctc tcggaagccg gccgctccct cttcagcgca tcgcgctcgc
     1681 gccgatcgtc ggcgaacgac gcggaccggc tgcgaaaata tctgcagcgc tacggactgg
     1741 attgggcggg gctgcaggaa attctccgtc attccggcga aggccggaat ccatgaacgc
     1801 ggctcagcta ggcttgaaag gccggtgtgt tcatggattc cggcctcgcc gcgatgacga
     1861 gggtgcgaaa tgcggcgggc gcggactcaa ggccctagcc aaaggcctct aattaaggca
     1921 ttatattcat t
//
LOCUS       DASWNU010000286         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_219125, whole genome shotgun sequence.
ACCESSION   DASWNU010000286 DASWNU010000000
VERSION     DASWNU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219125"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2116
                     /gene="ligD"
                     /locus_tag="VGB57_05620"
     CDS             <1..>2116
                     /gene="ligD"
                     /locus_tag="VGB57_05620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY0130865.1"
                     /translation="HYDLRLELEGTLKSWAVTRGPSLNPEDKRLAVRTEDHPLSYATF
                     EGTIPKGEYGGGTVMLWDWGKWTPHPGKDPRKTLEEGHLHFTLDGHRMQGEWVMFRLK
                     GRPGERAENWMLKKVTDEHAGGSMGLTEKYLTSIKTGRSMQEIAAGKKAKELKGWKTD
                     PNKPSSRRKPGPQDGKAQEAARGPGFRRGDGKRSTKLPPFREPQKATLVDHVPSGSGW
                     IHEMKYDGYRCLLGIGGGKAKVYTRSGLDWSDKFPEIAEAAARLEVGSALLDGEIVAL
                     DEKGNTGFSALQQAISEGGRGLTLFLFDALEIDGEDLTALPTIERKGRLASLVGDGRP
                     PTILYAEHIVGHGEKLFDAMCEAGQEGIISKRADAPYRSGRTKSWLKIKCTYRQEFVI
                     LGWSESDKKGRGFRALLLGVHEDGKLRYAGKVGTGFSNQVQLDLSERFERLAAPKPAA
                     PVPRAEARGAHWVKPQLVCEVAFAEFTAEGVVRHASFLGLRGDKKASEVIEETPQPVE
                     RAAPAAGDGVKISNRERVLYPDSGVTKGELVDYYRTIGPMMMEWAADRPLSLVRCPQG
                     RARKCFFQKHNTGSFGPHVHQIPIEEKAGQIEDYVYVGESAGIIACTQMGTIEFHGWG
                     SSVADIEKPDRLVFDIDPDEGLSFDEVRRTARDLHRYLADMGLQTFPMVTGGKGLHVI
                     APLTPEAQWPQVKDFAQRFAIAL"
BASE COUNT          398 a          711 c          697 g          310 t
ORIGIN      
        1 ccactacgat ctccggctgg agctggaggg gacgctgaag agctgggccg tcacccgcgg
       61 cccgagcctc aatcccgagg acaagcgcct tgccgtccgc accgaggacc acccgttaag
      121 ctacgccacc ttcgagggca ccatccccaa gggcgaatat ggcggcggca ccgtcatgct
      181 ctgggactgg ggcaaatgga ctccccatcc gggcaaggat ccgcgcaaga cgctcgagga
      241 aggccatctc cacttcaccc tcgacggcca tcggatgcag ggcgaatggg tgatgttccg
      301 gttgaagggt cgacccggcg agcgcgccga gaactggatg ctgaagaagg tcaccgacga
      361 gcatgccggc ggctcgatgg ggctgaccga gaagtatctg accagcatca agacggggcg
      421 ctcgatgcag gagatcgccg cgggtaagaa ggctaaggag ttgaagggct ggaagacgga
      481 tcccaacaag ccgtcatccc ggcgaaagcc gggaccgcag gacggaaagg ctcaggaggc
      541 ggcccgaggc cccggctttc gccggggtga cggcaagcgc tctaccaagc tcccgccctt
      601 ccgcgagccg cagaaagcca ccctcgtcga tcatgtcccc tccggctccg gctggatcca
      661 cgagatgaaa tatgacggct atcgctgcct gctcggaatc ggcggcggca aggccaaggt
      721 atacacgcgc tccggcctcg actggtcgga caaattcccg gagatcgccg aggcagcggc
      781 gcggctggag gtcggttcgg ccctgctcga cggcgagatc gtcgccctcg acgagaaggg
      841 caataccggc ttctcggcgc tccagcaggc gatcagcgaa ggcggccgcg gcctcaccct
      901 cttcctgttc gacgcgctcg agatcgacgg cgaggatctg accgccctcc ccacgatcga
      961 gcgcaagggc cgcctcgcct cgctcgtcgg cgacggcagg ccgccgacca tcctctatgc
     1021 cgagcatatc gtcggccatg gcgagaaatt gttcgacgcg atgtgcgagg ccggccagga
     1081 gggcatcatc tccaagcgcg ccgacgcccc ctatcgaagc ggccgaacca agagctggct
     1141 caagatcaaa tgcacctacc gccaggagtt cgtcatcctc ggctggtccg aaagcgacaa
     1201 gaagggccgc ggcttccggg cgctgctgct cggagtccat gaggatggca agcttcgcta
     1261 cgccggcaag gtcggcaccg gcttctccaa ccaggtccag ctcgacctga gcgagcgctt
     1321 cgagaggctg gccgcaccca agccggccgc tcccgtgccc agggccgaag cgcgcggcgc
     1381 ccattgggtg aagccgcaac tggtctgcga ggtcgccttc gccgagttca ccgccgaagg
     1441 cgtcgtgcgc cacgccagct tcctcggcct gcgcggcgac aagaaagctt ccgaggtgat
     1501 cgaggagacg ccccagccgg tcgagcgcgc cgcgcccgcc gccggcgacg gggtcaagat
     1561 cagcaaccgc gagcgggtgc tgtaccccga ctccggggtc accaagggcg agctggtcga
     1621 ttattatcgg acgatcggcc cgatgatgat ggaatgggcc gcggatcgtc cgctcagcct
     1681 ggtccgctgc ccgcagggcc gcgcccgcaa atgcttcttc cagaagcaca atacgggcag
     1741 cttcggcccc catgtccacc agatcccgat cgaggagaaa gcgggccaga tcgaggatta
     1801 tgtctatgtc ggggagtcgg cggggatcat cgcctgcacc cagatgggga cgatcgagtt
     1861 ccacggctgg ggctcgtcgg tggcggacat cgagaagccc gatcgtctgg tcttcgacat
     1921 cgatccggac gagggcctca gcttcgacga ggtcaggcgc accgcccgcg acctccaccg
     1981 ttacctcgcc gacatgggcc ttcagacctt cccgatggtg accggcggca agggtcttca
     2041 cgtcatcgcg ccgctgaccc cggaagccca gtggccgcag gtaaaggatt tcgcgcagcg
     2101 cttcgcgatc gcgctc
//
LOCUS       DASWNU010000287         5343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_347716, whole genome shotgun sequence.
ACCESSION   DASWNU010000287 DASWNU010000000
VERSION     DASWNU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347716"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1436)
                     /locus_tag="VGB57_05625"
     CDS             complement(108..1436)
                     /locus_tag="VGB57_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0130866.1"
                     /translation="MKPILFALAAISLLGGCGSLGIGGKGKKNVTPTVGERISVLSRE
                     RVLEVDPTLSAIAVTLPPAQANSEWTQSGGNAAKSMGHLALGATPAMAWRVSTGEGSS
                     NQAQLASSPVIGEGRIFTIDTRAVVRAISPENGATVWQAQVRGANAPEGALFGGGVAY
                     DNGRIYATNGAGDAAALDAKTGAVIWRVRPGGPLRGAPTVANDNVYVVTQDNQLFALN
                     PANGTTRWSGSGAVEIAGILGASSPAAAQGTVVAGFSSGELNAYRYENGQNLWQDALA
                     RTSISTSVTQLSDIDADPVIDSGRVYAVGQGGRMVAIELITGQRVWEINVAGISTPWV
                     AGDWIFVVTDEAQLLALSRTTGQIRWMTQLTRYRDVKDKKGRIDWVGPILAGDRLVMA
                     NSVGQLVYVSPYDGRVQTTQDTRMSVSLQPIVANNTLYVLHDNGQLTAWR"
     gene            complement(1433..2143)
                     /locus_tag="VGB57_05630"
     CDS             complement(1433..2143)
                     /locus_tag="VGB57_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0130867.1"
                     /translation="MALRPVDNESFYREVDEELRRDQMKTYWERFGKLVIAGVVLLLA
                     LLGGFIWWQNQKEVKAGERSAALVEAFDDVAVGRKAAATAKLDTLAREGSEGHRAAAL
                     LTKADIAIEGKDLKGAAALYKQIADDEGLARPYRDLALVRMTAVELDTLPPQAVVDRL
                     KGLAVAGNPWFGSAGEMVALSYLKLNKPQQAAGVFAAMAKDKKLPDTLRSRATQMAGS
                     LGVDAVQEAAGSTQEDTQ"
     gene            complement(2377..3237)
                     /gene="panB"
                     /locus_tag="VGB57_05635"
     CDS             complement(2377..3237)
                     /gene="panB"
                     /locus_tag="VGB57_05635"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY0130868.1"
                     /translation="MSVTHSLDTATSRANPVPSPMKRLTVPKLKAMKGGEPIVMLTAY
                     TARLAQLLDPHCDILLVGDSLGQVVYGLPSTVPVTLEMMCAHGAAVVRGSWHSVVAVD
                     MPFGSYEASPQQAFASASRILKETGAAAVKLEGGEAMAETIAFLTRRGIPVIGHVGLT
                     PQAVNVLGGYGTRGRGEAEARKIVADAKSVDEAGCFLLVLEGVMEEVAAEITGLVSCP
                     TIGIGASPSCDGQVLVTEDMLGMFERTARFVKRYDDLAGRIETAAQAYAGEVRSRVFP
                     GEEQIQRARK"
     gene            complement(3247..4107)
                     /locus_tag="VGB57_05640"
     CDS             complement(3247..4107)
                     /locus_tag="VGB57_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0130869.1"
                     /translation="MSATLLAICLGLFSAITLAAANMSVKMGSDILAGRALLSTSAAL
                     MVAPAAFFVPLPDAWTWGALLLAVPAHYAYQLCLIRALQRGDLSLVFPVMRGAAPLLT
                     AAAAFLFLHEALPPLALAGLVVATAAVFVFAAPPSGTLLRHHPDGAVLAWALATAVGV
                     ALYNVADARGVRGAPEPFTYIVWIFLLDSICITCTALLRRRRELGRTIACKWRFGVAA
                     GFLSILSYGSAVYAFSLMETARVSALRETSVVFAAMLGALFLKEGFGARRIAAALALA
                     AGLVAMQFGG"
     gene            4302..5261
                     /locus_tag="VGB57_05645"
     CDS             4302..5261
                     /locus_tag="VGB57_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130870.1"
                     /translation="MRNRILMMALATAGITVGYATPAAAQATRTWVSGVGDDVNPCSR
                     TAPCKTFAGAISKTAAGGEINCLDPGGYGAVSIIKSMTISCPYTEGGALAGGNGITVN
                     MPLATDVTVLRGLDIFGVNPPSNGVRFINNGSLHIEDSVIRRFNAANSAGVSVGAAGA
                     VNLFISNTTITQNGNGATGGGIVIAPAAGGSIKAVLNNVRVVNNANVGVRIDSTNTVS
                     ANGLLTTIVNSEISGNPTGLNATGGGTGNNVMVTRSTIAVNGTGVNTNGATAVVRMGT
                     TNVSGNQTGITASGGGVNRSYEDNQVNGNIVDGSFNGAVLTEI"
BASE COUNT          938 a         1768 c         1818 g          819 t
ORIGIN      
        1 gcggtccccc tccccgcgaa atcgcgggga ggactaaaat cctccccggc attcgccggg
       61 gagggggacc agccgcaggc tggtggaggg gttctttcct cgagtttcta ccgccaggcc
      121 gtgagctgcc cgttgtcgtg gaggacgtag agggtgttgt tggcgacaat gggctggagc
      181 gagaccgaca tcctcgtgtc ctgggtcgtc tggacccgcc cgtcgtaggg cgagacatag
      241 acgagctgcc cgacggaatt ggccatcacc aggcggtcgc cggcgaggat cggcccgacc
      301 cagtcgatcc gccccttctt gtccttgacg tcgcgatagc gggtcagctg ggtcatccac
      361 cggatctggc cggtggtccg cgacagggcg agcagctggg cttcgtcggt gacgacgaag
      421 atccagtcgc ccgccaccca gggggtcgag atgccggcga cgttgatctc ccagacgcgc
      481 tggccggtga tcagctcgat cgcgaccatc cggccgccct ggccgaccgc atagacgcgg
      541 cccgagtcga tcaccggatc ggcgtcgatg tcggaaagct gggtgacgct ggtcgagatg
      601 ctggtccggg cgagcgcgtc ctgccacaaa ttctgcccgt tctcgtagcg ataggcgttg
      661 agctcgcccg acgagaatcc ggcgaccacc gtcccctgcg ccgcggccgg cgaagaggcg
      721 ccgaggatgc cggcgatctc gaccgcgccc gagccgctcc agcgagtcgt gccgttggcg
      781 ggattgagag cgaacagctg gttgtcctgg gtgacgacgt agacgttgtc gttggcgacc
      841 gtcggggcac cgcgcagcgg cccgcccggg cgaacccgcc agatgaccgc tccggtcttc
      901 gcgtcgagcg ccgccgcgtc gccggcgccg ttggtcgcgt agatccggcc gttgtcgtag
      961 gcgactccgc cgccgaacaa ggcgccctcg ggcgcattgg cgccgcgcac ctgggcctgc
     1021 cagacggtgg cgccgttctc ggggctgatc gcccggacca cggccctggt atcgatggtg
     1081 aagatccggc cctcgccgat gacgggcgag gaggcgagct gggcctggtt gctgctgccc
     1141 tcgcccgtgc tcacccgcca ggccattgcg ggggtggcgc cgagggcgag atggcccatc
     1201 gacttggcgg cattgccgcc cgactgggtc cattccgaat tggcctgcgc cggcggcagg
     1261 gtcaccgcga tcgcgctcag ggtgggatcg acctccagca cgcgctcacg cgagaggacg
     1321 gagattcgct cgccgaccgt cggcgtcacg ttcttcttgc ctttgccgcc gatgccgagg
     1381 gagccgcagc cgccgagcag gctgattgcg gcgagggcga aaagaatagg cttcattgag
     1441 tgtcttcctg tgttgagccc gccgcctcct ggacggcgtc gactccgagc gaaccggcca
     1501 tctgggtggc ccgggaacgc aaggtgtcgg ggagcttctt gtccttggcc atggcggcga
     1561 agacgccggc ggcctgctgc ggcttgttga gcttgagata ggagagcgcg accatctcgc
     1621 cggcgctccc gaaccagggg ttgccggcga cggcgagccc tttcagccga tcgactaccg
     1681 cctgaggcgg gagggtgtcg agctcgaccg ccgtcatcct caccagagcg aggtcgcgat
     1741 agggccgggc gagaccttca tcgtcggcga tctgcttgta gagcgcggcc gcgcccttca
     1801 gatccttgcc ctcgatggcg atgtcggcct tggtgagaag agcggcggcg cgatggccct
     1861 ccgatccttc cctggcgaga gtgtcgagct tggcggtggc ggcggccttc cggccgacgg
     1921 caacgtcgtc gaaggcttcg accagcgccg cgctgcgctc cccggctttc acctccttct
     1981 ggttctgcca ccagatgaag ccgccgagca gggccagcag caggaccact ccggcgatga
     2041 cgagcttgcc gaaccgctcc cagtaggtct tcatctggtc gcggcgaagc tcttcatcga
     2101 cctcgcggta gaaggattcg ttgtcgacag gcctcagcgc caagaaaaac ctccaaatgc
     2161 tgtccggtcg ccccccgccc gcccaagcct catcgccgat gaaagcgata ctgaggatga
     2221 acgaaaaagc gcggcgccgg catctgcccc gggcgcttct ctaccgaggg gcggcctgca
     2281 acggaagcct tcattgtcgc gaagccgttc ccgggccgag gccagagttc agccctgcag
     2341 gatccggatc ccggccttcg ccgggcaaca tggacctcac ttcctggcgc gctggatttg
     2401 ctcctcgccg ggaaagacgc ggcttcgaac ctcccccgca taggcctgcg cagcggtctc
     2461 gatccggccg gcaaggtcgt catagcgctt cacgaagcgc gcagtgcgct cgaacatgcc
     2521 gagcatgtcc tcggtgacca gcacctggcc gtcgcaactc ggcgaggcgc cgatgccgat
     2581 ggtcgggcag gagaccagcc cggtgatctc cgccgcgacc tcctccatca ctccttcgag
     2641 gacgagcagg aagcagcccg cctcgtcgac cgacttcgca tcggccacga tcttgcgagc
     2701 ctccgcctcg cctcgccccc gagttccata gccgcccagc acattcaccg cctgaggggt
     2761 caagccgaca tggccgatca ccggaattcc gcgccgggtg aggaaggcga tcgtctcggc
     2821 catggcctcg ccgccctcga gcttcaccgc cgccgcgccg gtttccttga ggatccgcga
     2881 ggcgctggcg aaagcctgtt gggggctcgc ttcataggaa ccgaagggca tgtcgacggc
     2941 gacgaccgag tgccaggaac cgcggaccac cgcggcgccg tgggcgcaca tcatctcgag
     3001 agtgaccggg acggtggagg gaagaccgta gacgacctgg ccgaggctgt cgccgaccag
     3061 caatatatcg caatgcggat cgagcagctg ggcgaggcgc gccgtgtagg cggtcagcat
     3121 gacgatgggc tcgccgccct tcatcgcctt aagcttcggc accgtcagcc gcttcatcgg
     3181 gcttgggacg ggattggcgc gactggtcgc ggtatccagc gaatgggtga cggacatatc
     3241 cgtctcttag ccgccgaact gcatcgcgac cagcccggcc gccagggcga gagccgcggc
     3301 gattcgacgc gcgccgaatc cctccttcag gaacaaggct ccgagcatgg cggcgaagac
     3361 caccgacgtt tcgcgcaggg ccgaaaccct ggcggtctcc atcaggctga aggcgtagac
     3421 ggccgaaccg taggaaagga tggagaggaa gcccgcggcg actccaaagc gccatttgca
     3481 ggcgatggtc cgcccgagct cccgacgccg gcgaagcagg gcggtgcagg tgatgcagat
     3541 ggaatcgagc aggaatatcc agacgatata ggtgaagggc tccggagcgc cgcggacgcc
     3601 gcgagcgtcg gcgacgttgt agagggcgac tcccaccgcg gtggcgagcg cccaggcgag
     3661 caccgcccca tcggggtggt gacgcagcaa tgtaccgctc ggcggcgccg cgaagacgaa
     3721 gacggcggcg gtcgccacca ccagcccggc cagcgccagc ggcggcagag cctcgtggag
     3781 gaagaggaag gcggcggcgg cggtgagcag cggcgcggcg ccccgcatca ccggaaagac
     3841 cagcgacagg tcgccgcgct gaagcgcccg gatcaggcag agctggtagg cgtaatgggc
     3901 ggggaccgcg agcagcagcg ctccccaggt ccaggcgtcc ggcagtggca cgaaaaaggc
     3961 cgcgggggcg accatcaagg cggcgctggt ggacagcaac gcacgtccgg cgaggatgtc
     4021 gctccccatc ttcaccgaca tgttcgcggc ggcgagggtg atggccgaaa acaatccgag
     4081 gcagatggcg aggagggtcg cgctcacgcc cgagtcgccg ttgcacgggt cgactgccgc
     4141 ggaatcggcc cccgcggaga atcgtcggcc cggtaaacgc cgcggacgcc atctaagccg
     4201 ctgccatgat taagcatttt taggataaat tgtaggagta gaatgatccg ttttgtgata
     4261 ttcatttgga gttcattcaa tccaaaatgg gggataagca aatgagaaat cgtatcctga
     4321 tgatggcgct cgccaccgcc ggaattaccg tcggctatgc cactccggcc gccgcccagg
     4381 cgacccggac ctgggtgtcg ggcgtcggcg acgacgtcaa tccgtgcagc cgcaccgcgc
     4441 cctgcaagac ctttgccggc gccatctcga agaccgccgc cggcggcgag atcaactgcc
     4501 tcgatccggg cggctacggc gcggtgtcga tcatcaagtc gatgaccatt tcatgcccct
     4561 ataccgaggg cggagcgctc gccggcggca acggcatcac ggtcaacatg cccctcgcga
     4621 cggacgtgac ggtccttcgg ggcctcgaca ttttcggggt caacccgccg agcaacggcg
     4681 tccgtttcat caacaacggc tcgctccaca tcgaggattc cgtaatccga cgcttcaacg
     4741 cggcgaacag cgcgggcgtg tccgtcggag ccgccggcgc cgtgaacctg ttcatcagca
     4801 acacgacgat cacccagaac ggtaacggcg ccaccggcgg cggaatcgtg atcgcgcccg
     4861 cggccggggg cagcatcaag gcggtcctca acaacgtgag ggtggtgaac aacgccaatg
     4921 tcggtgtcag gatcgactcc acgaacacgg tctccgcgaa cggcctgctg acgacgatcg
     4981 tcaacagcga gatttcggga aatccgaccg ggctcaacgc cacgggcggc ggaaccggca
     5041 acaacgtgat ggtgacccgc tcgacgatcg ccgtcaacgg gaccggggtc aatacgaacg
     5101 gcgcgaccgc cgtggtgcgc atggggacga cgaacgtctc cggcaaccag accgggatca
     5161 ccgcgtcggg cggcggcgtg aaccgcagct acgaagacaa tcaggtgaac ggcaacatcg
     5221 tcgatggctc gttcaacggc gccgtcctca ccgaaatcta agcggacgaa acgaggggag
     5281 gcgggttcga tcgggcccgc ctcccgtcac tcccactcga tcgtgcccgg cggtttcgac
     5341 gta
//
LOCUS       DASWNU010000288         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_426249, whole genome shotgun sequence.
ACCESSION   DASWNU010000288 DASWNU010000000
VERSION     DASWNU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426249"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VGB57_05650"
     CDS             <1..765
                     /locus_tag="VGB57_05650"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0130871.1"
                     /translation="AAGVPANKVGLIVLATATPDQTFPASATRVQTALGINDCIAFDV
                     AAVCTGFLYALSVADNMLKGGMADYALVIGSETFSRILDWEDRTTCVLFGDGAGAILL
                     HSVEAQDKGVLATKLHADGRHNDLLFVDGGASTTGTIGKLRMKGKEVFRHAVVNLAAV
                     LNETLEAAGHSADEVDWVVPHQANKRILDATAKKLGLDPARVIVTVDQHANTSAASVP
                     LALDTAVKDGRIKPGDLIVLEAMGGGFTWGAAVVRY"
     gene            886..1209
                     /locus_tag="VGB57_05655"
     CDS             886..1209
                     /locus_tag="VGB57_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY0130872.1"
                     /translation="MADAGTIRRADAGTLTRADLADVIHREIGLSRAESAGIVERILH
                     HMCHALSEGQNVKISGFGSFILRDKGERVGRNPKTGVEVPIAPRRVLTFRASQIMRER
                     IAKGD"
     gene            1221..1598
                     /locus_tag="VGB57_05660"
     CDS             1221..1598
                     /locus_tag="VGB57_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0130873.1"
                     /translation="MADASGKSEQAFRTIGELASELDVPQHILRYWESRFPQLKPLQR
                     AGNRRYYRPADVALARRIHRLLNQDGYTIRGVQQLLASGGADDSAHAVEAIPEPARPV
                     GRSTLPLEQLKAIRQTLAEALDA"
     gene            complement(1636..2172)
                     /locus_tag="VGB57_05665"
     CDS             complement(1636..2172)
                     /locus_tag="VGB57_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0130874.1"
                     /translation="MKTLTLLRHAKSGWDDPVSRDFDRPLNPRGRRAARTVAREMKAQ
                     GLAFDLVLASPARRVIETLEEVAAGYGAIQPRYDQRLYLASTATLIEIVRATPDEVER
                     LLLVGHNPGLEELALCLSRGDEAGLRGEVEVKYPTATVAEIGLPVERWAEVRERTGSL
                     VRFIRPRDLDPELGPDSE"
     gene            complement(2169..>4320)
                     /locus_tag="VGB57_05670"
     CDS             complement(2169..>4320)
                     /locus_tag="VGB57_05670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0130875.1"
                     /translation="RDAEVREGQRDYAAAAAGTFDPRAAEGQPNMLLAEAGTGIGKTL
                     GYLAPASLWAESADGAVWVSTFTKALQRQLDREGLRLFPDAEARRRKVVVRKGRENYL
                     CLLNLEDALQGGFAGRAAILAQLVARWAAFSKDGDMVGGDLPGWLPTLFRRAGATALT
                     DRRGECVYAGCPHYRKCFIERSARASQDADIVIANHALVMVNAARGRDGGNPPTRIVF
                     DEGHHLFEAADSTFAAALTGQEAIELRRWIVGPESKSRGRRRGLAARLTDVASYDEAG
                     ALALEMAVQAAGLLPSDGWLQRIAEGMPFGPLEKLLAAVRGTAYARATAQDAGYGIET
                     EMAELDGDIVAAAAPAVEALEQLMRPLMALARRLEAVIEDSPDWLDSQARARIEGAIG
                     GLAWRTQTIAAWVALAARLGGPADPDFVDWLAVERIDGREYDIGLHRHWLDPTRPLAD
                     IVLKPAHSVIVTSATLRGAEDWESAEARTGAAHVAGPVRRFSAPSPFDYASNSEVLIV
                     TDVKRGDIGQLAGAYSRLVEAAEGGTLGLFTAIQRLKAVHARIADRLARAGLPLYAQH
                     VDPIDTGTLVDIFRDDPHASLLGTDALRDGVDVPGHSLRLVVMEGVPWPRPTVLHSAR
                     RAAGGGTAYDDRVVRARLAQAFGRLIRRKEDRGVFVLLSAATPTRLLSAFPPGTPIRR
                     LTLVEAVERVRSRLSTRPAFGHQALHERRSEEAE"
BASE COUNT          659 a         1538 c         1485 g          638 t
ORIGIN      
        1 gccgccgggg ttccggcgaa caaggtcgga ctgatcgtgc tcgccaccgc cacgcccgac
       61 cagaccttcc cggcgagcgc gacccgggtc cagaccgcgc tcggcataaa cgattgcatc
      121 gccttcgacg tcgccgccgt ctgcaccggc ttcctctatg ccctgtcggt cgcggacaat
      181 atgctgaagg gcgggatggc cgattacgct ctggtgatcg gctcggagac gttcagccgg
      241 atcctcgact gggaggaccg gacgacctgc gtcctgttcg gcgacggcgc cggagcgatc
      301 ctccttcatt cggtcgaggc ccaggacaag ggcgtgctgg cgacgaagct ccatgccgac
      361 ggccgccaca acgaccttct cttcgtcgac ggcggcgcct cgacgacggg gacgatcggc
      421 aagctgagga tgaagggcaa ggaagtcttc cgccacgccg tcgtcaacct cgccgccgtt
      481 ctcaacgaga ctctcgaggc cgccggccac agtgccgacg aggtcgactg ggtggtcccg
      541 catcaggcca acaagcgcat cctcgacgcc accgcgaaga agctcgggct cgatccggcg
      601 cgggtgatcg tcaccgtcga ccagcacgcc aacacctccg ccgcctcggt gcctctggcg
      661 ctcgacaccg cggtgaagga cgggcggatc aagccgggcg acctgatcgt gctcgaggcg
      721 atgggcggcg gcttcacctg gggcgccgcc gtcgtccgtt attgagggtc catgccgata
      781 cgtaataccc gaagacgtat ttagggcttt cacccgtatt gccggtttgc aactaagtgc
      841 aagctggact atggtttttc attgcttccg ggggagggag aacaaatggc cgacgctgga
      901 accatcaggc gagcagacgc cgggaccttg acgcgcgccg accttgcgga cgtcattcat
      961 cgcgagatcg gtctttctcg ggccgaatcg gccggaatcg tcgagcggat ccttcatcat
     1021 atgtgccacg ctctgtccga agggcagaac gtgaagatct ccggcttcgg aagcttcatc
     1081 cttcgcgaca agggcgagcg cgtcggccgc aaccccaaga cgggcgtcga agtgccgatc
     1141 gcgccgcgcc gggtgctcac cttccgcgcc agtcagatca tgcgcgagcg gatcgccaag
     1201 ggcgactgag gccgcgcgcc atggcggacg cctcaggcaa gagcgaacag gctttccgca
     1261 cgatcgggga gctcgcgtcc gagctggacg tgccgcagca catcttgcgc tattgggaga
     1321 gccggttccc gcagctgaag ccgcttcagc gcgccggcaa ccgccgctat taccggccag
     1381 cggacgtggc tttggcccgc cgtatccacc gcctcctgaa ccaggacggc tatacgatcc
     1441 gcggagtcca gcaattgctg gcgagcgggg gcgcggacga ttccgcccat gccgtcgagg
     1501 cgattcccga gccggcgcgc cccgtcggcc ggagcacgct tccgctcgag cagctgaagg
     1561 ccattcgcca gactttggcc gaggcgctcg acgcgtagcc gccgtcatcc cggctttcgc
     1621 cgggatgacg agctgctatt cggagtccgg cccgagctcc gggtcgaggt ccctcggccg
     1681 gatgaagcgg accaggcttc cggtcctctc cctcacctcc gcccaacgtt ccaccggcag
     1741 cccgatctcc gccaccgtag ccgtcggata tttcacctcg acctccccgc gcagccccgc
     1801 ctcgtcgccg cgggagaggc acaaggcgag ctcctccagc cccggattgt ggccgaccag
     1861 aagcagccgc tcgacctcgt ccggagtcgc tcggacgatc tcgatcagag tcgcggtcga
     1921 ggccaggtag aggcgctggt catatcgcgg ctggatcgcg ccataaccgg ccgccacctc
     1981 ctccagagtc tcgatcaccc gccgcgccgg cgacgcgagc accaggtcga aggccaggcc
     2041 ctgcgccttc atctcgcggg ccaccgtccg ggccgccctc cggccgcgcg ggttgagcgg
     2101 ccggtcgaag tcgcggctga cgggatcgtc ccagccggac ttggcatggc gcagcagcgt
     2161 cagtgtcttc attcggcttc ttcggaccgc ctctcgtgga gcgcctgatg cccgaaagcc
     2221 ggccgggttg aaagccgcga ccgcacccgc tccaccgctt cgaccagggt gagccgccgg
     2281 atcggcgtgc cgggcggaaa ggccgagagc agcctcgtcg gagtcgcggc ggaaagcagg
     2341 acgaaaacgc cccgatcctc ctttcggcgg atcagcctcc cgaacgcctg ggcgagccgg
     2401 gcccggacca cccggtcgtc ataggcggtg ccgccgcccg ccgcccgccg ggccgagtga
     2461 agcacggtcg gccgcggcca gggcacgccc tccatcacca ccagccggag cgaatggccg
     2521 gggacgtcga ccccgtccct cagagcgtcg gttccgagca gcgacgcgtg cggatcgtcg
     2581 cggaagatgt ccaccagggt gcccgtgtcg atcgggtcga cgtgctgggc atatagcggc
     2641 agcccggcgc gggcgaggcg gtcggcgatc cgcgcgtgga ccgccttcag ccgctggatg
     2701 gcggtgaaca ggcccagcgt cccgccttcc gccgcttcga ccagccggga ataggcgccg
     2761 gcgagctggc cgatgtcgcc gcgcttgacg tcggtgacga tcaggacctc gctgttcgag
     2821 gcataatcga atgggcttgg ggcgctgaac cggcgcaccg gcccggcgac gtgggcggcg
     2881 ccggtccggg cctcggcgct ctcccagtcc tccgcgcccc gcagggtcgc ggaagtgacg
     2941 atgacgctgt gggccggctt caggacgatg tcggcgagcg gccgggtcgg gtcgagccag
     3001 tggcgatgga ggccgatatc atattcgcgg ccgtcgatgc gctcgaccgc gagccagtcg
     3061 acgaagtccg gatcggccgg gccgccgagc cgcgccgcaa gcgccaccca ggccgcgatc
     3121 gtctgggtcc gccaggcgag ccctccgatc gccccctcga tgcgggcccg ggcctgcgaa
     3181 tcgagccagt cgggcgagtc ctcgatcacc gcttccagcc gccgggcgag cgccatcagc
     3241 ggccgcatca actgctccag cgcttccacc gcgggagcgg ccgcggcgac gatgtcgccg
     3301 tccagctcgg ccatctcggt ctcgatcccg tagccggcgt cctgggcggt ggcccgggcg
     3361 taggcggtgc cgcgcaccgc cgcgagcagc ttctccagag ggccgaacgg catgccctcg
     3421 gcgatccgct gaagccagcc gtcgctcggc aggagccccg ccgcctggac cgccatttcg
     3481 agcgcgagcg ctccggcctc gtcataggag gcgacatcgg tcagccgggc cgcaagcccg
     3541 cgccgccggc cgcgcgactt gctctcgggt cccacgatcc agcgcctgag ctcgatcgcc
     3601 tcctgcccgg tcagggccgc ggcgaaggtc gaatcggcgg cctcgaacag atgatggcct
     3661 tcgtcgaaga cgatccgggt gggcgggttc cccccgtccc ggccgcgggc ggcgttgacc
     3721 atcaccaggg cgtggttggc gatgacgatg tcggcatcct ggctcgcccg cgccgagcgc
     3781 tcgatgaagc atttgcggta atgcgggcag cccgcataga cgcattcgcc gcggcggtcg
     3841 gtgagcgcgg tcgccccggc gcgtcggaac agggtcggga gccagcccgg aaggtcgccg
     3901 ccgaccatgt cgccgtcctt cgagaaagcg gcccagcggg cgacgagctg ggcgaggatg
     3961 gcggcccggc ccgcgaagcc gccctgaagc gcatcctcca gattgagcag gcagaggtag
     4021 ttttcacgcc ccttgcggac gacgaccttg cgccgccgcg cctccgcgtc cgggaacagg
     4081 cgaaggccct cgcggtcgag ctggcgctga agcgccttgg tgaaggtcga gacccagacc
     4141 gccccgtcgg cgctttcggc ccagagcgag gccggtgcga gatagccgag ggtcttgccg
     4201 attccggtgc ccgcttcggc gagaagcatg ttgggctggc cctcggcggc gcgcggatcg
     4261 aaggtgccgg ccgccgcggc ggcataatcg cgctgtccct cgcggacctc ggcgtcccgg
//
LOCUS       DASWNU010000289         7219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_115238, whole genome shotgun sequence.
ACCESSION   DASWNU010000289 DASWNU010000000
VERSION     DASWNU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115238"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VGB57_05675"
     CDS             <1..1491
                     /locus_tag="VGB57_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0130876.1"
                     /translation="DLRGVRSWIDAQPEWSDLPFVLLTGRGGGLERNPAAERFLEVLG
                     NVTFLERPFHPTTLVSVARSALRGRRRQYEARARLESLAASQRALTLSNETLEARVEE
                     RTREHESALAQLHEAQKLETLGQLTGGVAHDFNNLLTPIIGSLDLLRRRAPPADKSHR
                     HIDMALQAASRAATLVQRLLAFARRQDLQPRSVDIVALLRGMQDLIRKSVGPMVEVAV
                     DCPAELPPARIDPNQLELAILNLALNARDAMARGGRLSISARAEQVGPGRRLAPGEYL
                     RVAISDTGVGMDSITLGHAVEPFFSTKGVGRGTGLGLSMVHGLCAQLGGTLFLRSKPG
                     EGTTAEMWLPAAAEPAGREETEQAPLMVARRAATILLVDDEDIVRRATADMLADIGYT
                     VIEAASGAEALRMVREGTICDGVVSDYLMPGMNGVDLIRHLRDLAPGLPAMLISGYST
                     IAEGLGSELPRLAKPFRQADLASSVAQLLAEPAAGTIKFPVERTRR"
     gene            1555..2316
                     /locus_tag="VGB57_05680"
     CDS             1555..2316
                     /locus_tag="VGB57_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0130877.1"
                     /translation="MTNPWTMKMEQFTSFTEEQRARLDALVAARRQEYAPDEDILVED
                     QPINECHVLISGLAARYKLLPDGERQIMAFLIPGDLCDAEVFILEKMDHSVCAITPTT
                     CALVSADTMRGLLREISTMSEALWWGTMTDLAVLRERLVDLGRREARERIAHLIYEML
                     IRYRIVGETSDDVMPWPITQDELADATGLTPVHVNRTLKQLREEGLIEVTRRELTVLE
                     PDRLREVAQFNPNYLHLIRTEKGEGRVAERAGDLV"
     gene            2331..2576
                     /locus_tag="VGB57_05685"
     CDS             2331..2576
                     /locus_tag="VGB57_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130878.1"
                     /translation="MPTYYFHLHDRWGSTPDETGREFADPGAVRKHALKAARAMIAED
                     VSKGCLDLRGRIEVTDNVGNLLLMLPFADAVERLDGN"
     gene            2730..3503
                     /locus_tag="VGB57_05690"
     CDS             2730..3503
                     /locus_tag="VGB57_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0130879.1"
                     /translation="MSPFRPSVLAAMLPKLERWTPLGPDDRSAIAALPHKVRRVGPSH
                     YVVREGDRTENSCLLLSGFAYRSKIVGDGGRQIVALHVKGDMIDLQNSLLGIADHNVQ
                     ALTAIEIAVIPREAILKLADERPAVARAMWHDTLVDGSVQREWTANIGRRDSKTRMAH
                     LLCEFALRLENAGIGQLCNYELPMTQEQLADCLALTPVHVNRTLKLLEKEALISRTVR
                     SVKIEDWEKLAREGDFNTTYLHVDLQGRSIDPVPALRLA"
     gene            complement(3911..4123)
                     /locus_tag="VGB57_05695"
     CDS             complement(3911..4123)
                     /locus_tag="VGB57_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEY0130880.1"
                     /translation="MGPKPSGGYRVVIDKVVEEPDELLAFVRFVSPGRRCGVIAALTS
                     PVDIVRLPVSPKNVRWVIERQSSDCP"
     gene            complement(4138..4884)
                     /locus_tag="VGB57_05700"
     CDS             complement(4138..4884)
                     /locus_tag="VGB57_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130881.1"
                     /translation="MRPIRTILASFALALLSGTPSAAAQSQDRGGWRTQFEREAEPAL
                     GPAALAQVRAARTALLVRGPVSFPYTVRQADGSYRQQRPAFVAAVRTAGGWVRLSGDG
                     RRESLDAAAGRALDRLLAGRALWAEPAPDSRCTDPGGALVLARHQGRERFSAFPCGFA
                     GRTGEAARIVLAGRIDDWSRVPAEERPAGLPLVRFDERTASAFRHMSGLYQERMMAIR
                     RPAEWQGQWSRITARQGDPPPLPRSIFGAR"
     gene            complement(4965..6293)
                     /locus_tag="VGB57_05705"
     CDS             complement(4965..6293)
                     /locus_tag="VGB57_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY0130882.1"
                     /translation="MTATRPRRFYRSLYFQVLVAIALGILVGWLFPAFGASLKPLGDA
                     FIRLVKMIITPVIFLTVTTGIAGMSDLKAFGRVGAKAMAYFLTFSTLALAVGLIVANV
                     VRPGAGLNVDPASLDQGAVATYVDKAHDQSITAFLLDIIPDTFFSAFTSGQILQVLFV
                     SILFGIALGFLGERGERVLGGMRVLTAVMFRIVHILMYAAPIGAFGAMAYTIGAHGID
                     TLRQLITLVATFYATSLIFVLVVLGLVARAAGFSIFALIRYLKDELLLVLGTSSSESA
                     MPLLMEKLERAGCPKPIVGLVVPTGYSFNLDGTNIYMSLAALFIAQATDTPLGFADQM
                     LLMAVAILSSKGAAGVTGSGFIVLAATLSVLPTVPVAGMALILGVDRFMSECRSLTNF
                     IGNAVATIVVARWEKAVDLGHLRAALAGRGGEFEEAESDALQIEGRSDVA"
     gene            complement(6290..>7219)
                     /locus_tag="VGB57_05710"
     CDS             complement(6290..>7219)
                     /locus_tag="VGB57_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0130883.1"
                     /translation="RPQVGALAGAFLVALIALPLAWTSLPDQARDSRPDRRIESGDPA
                     AIANSPGDPGTPPSTADAAPDLAKPGRAELPPAGTADMPVPAPGGDTLADVVPAESGE
                     AVVAGTRIRRPDTVSSVPVTEVGGDDIVVTGSRIRRPNLDSAVPITSVGGEEFFETGG
                     GRRGDWNACTVDDPKRDPRACRRDAASVARGASGKASAQLADGLARAWEGELEKAVGA
                     FDRAIALAPRSSAAYLNRGLARRGLGDLDGALADLDRAVAYAPQSARGYYHRSLLLRQ
                     RGDSRRARSDEERAIQLDPRYAAAVRAARRQTR"
BASE COUNT         1222 a         2507 c         2438 g         1052 t
ORIGIN      
        1 gacctgcgcg gcgtacgttc gtggatcgac gcccagcccg aatggtcgga cctgcccttc
       61 gtgctcctca ccggccgcgg cggcgggctc gagcgcaacc ccgccgccga gcggttcctc
      121 gaggtgctcg gcaacgtcac cttcctcgag cggcccttcc atcccaccac tttggtcagc
      181 gtcgcccgct ccgccctgcg cggccggcgc cggcaatatg aggcccgggc gcgcctcgaa
      241 tcgctcgccg caagccagcg cgcgctcact ctgtcgaacg agacgctgga ggcccgggtc
      301 gaggagcgca cccgcgagca cgaatccgcg ctcgcccagc tccacgaagc ccagaagctg
      361 gagacgctcg gccagctcac cggcggcgtc gcccacgatt tcaacaatct gctcactccg
      421 atcatcggca gcctcgatct tctccgccgc cgggccccgc ccgccgacaa gtctcaccgg
      481 catatcgaca tggcgcttca ggcggcgagc cgcgccgcga ccctcgtcca gcgcctgctc
      541 gccttcgcgc gccgccagga cctccagccg cgctcggtgg acatcgtcgc cttgctgcgc
      601 ggaatgcagg acctgatccg caagtcggtc gggccgatgg tcgaggtcgc ggtcgactgc
      661 ccggcggagt tgccgccggc gcggatcgac cccaaccagc tcgagctcgc catcctcaac
      721 ctggccttga acgcgcgcga cgccatggcg cgcggcggca ggctgagcat ctcggcccga
      781 gccgagcagg tcggccccgg tcggaggctc gcgccgggcg aatatctgcg ggtggcgatc
      841 agcgacaccg gagtgggcat ggactcgatc acgctgggcc atgcggtcga gcccttcttc
      901 tcgaccaagg gcgtcggtcg aggcaccggc ctcggcctgt ccatggtcca cggcctgtgc
      961 gcccagctcg gcgggaccct cttcctcagg agcaagccgg gggaggggac aacggccgag
     1021 atgtggctcc ccgccgccgc cgagcccgcc ggccgcgaag aaaccgagca ggcgcccctg
     1081 atggtcgccc gccgcgccgc gacgatcctg ctggtcgacg acgaggacat cgtccggcgc
     1141 gccaccgccg acatgctggc cgacatcggc tataccgtca tcgaggccgc ctccggggcc
     1201 gaggcgctcc ggatggtgcg cgaggggacg atctgcgacg gggtcgtcag cgactatctg
     1261 atgccgggca tgaacggggt cgacctgatc cgccacctgc gcgatctcgc tccggggctg
     1321 ccggcgatgc tgatcagcgg ctattcgacg atcgccgaag gtttgggctc agagcttccc
     1381 cgcctggcca agccgttccg ccaggccgat ctggcgagca gcgtggcgca gctgctggcg
     1441 gagccggcgg cgggcacgat caaattcccg gtggagcgca cgcggcgcta gctcctcccg
     1501 ccggctcctt ttccggcacc gttcgttcca catagatcaa ctaggcgagc tcgaatgacc
     1561 aatccctgga ccatgaagat ggagcagttc acctccttca ccgaggagca gcgcgcgcgg
     1621 ctggacgcgt tggtcgcggc gcggcggcag gaatatgccc ccgacgagga catcctggtc
     1681 gaagaccagc ccatcaacga atgccacgtc ctcatctcgg gcctggccgc aagatacaaa
     1741 ttgctccccg acggcgagcg ccagatcatg gcgttcctca tccccggcga cctttgcgac
     1801 gccgaggtct tcatcctcga gaagatggac cacagcgtct gcgcgataac ccccaccaca
     1861 tgcgccctgg tttccgccga tacgatgcgc gggctgctgc gcgagatatc gacgatgtcg
     1921 gaggcgctct ggtggggcac gatgaccgat ctcgccgtgt tgcgcgagcg gctggtggat
     1981 ctgggccgca gagaggccag ggaacggatc gcgcacctca tttacgagat gctgatccgc
     2041 taccggatcg tgggcgagac gagcgacgac gtcatgccct ggccgatcac ccaggacgag
     2101 ctcgccgacg cgaccggcct caccccggtg cacgtgaacc gaacgctgaa gcagcttcgc
     2161 gaggaaggcc tgatcgaggt cacgcgtcgc gagctgacgg tgctggagcc ggaccgtctg
     2221 agggaggtgg cgcagttcaa ccccaattac ctgcacctga tccggaccga gaagggcgaa
     2281 ggccgggttg ccgagcgggc cggcgatctc gtctagcggc cggatccgcc atgccgactt
     2341 actattttca tctccacgac cgctggggct cgacaccgga cgagaccggc cgcgagttcg
     2401 ccgatccggg ggcggtacgc aagcacgcgc tgaaggcggc ccgcgcgatg atcgcggagg
     2461 acgtcagcaa gggctgcctc gatttaaggg gcaggatcga ggtcaccgac aatgtcggca
     2521 acctgctcct gatgctgccc ttcgccgacg cggtcgagcg gctcgacgga aactaatttg
     2581 gattaggtag cgaagaacca acataaacga acatatcata gaattttggc cgattgcgtg
     2641 tggtaccgca atcacatcga gaggatttct tcggcccccc ccgacagcga gccatcatgc
     2701 tcccggccca ctaccgacag gagccgacga tgagcccatt cagaccctct gtgctggcag
     2761 cgatgctccc caagctggag cggtggactc cgctcggccc cgacgatcgc agcgcgatag
     2821 cggcgcttcc gcacaaagtc cgccgcgtcg gcccaagcca ttatgtggtg agggaggggg
     2881 acaggacgga gaattcgtgc cttctgcttt cgggcttcgc ctatcgatcg aagatcgtcg
     2941 gcgacggcgg ccggcagatc gtcgcgcttc acgtcaaggg ggacatgatc gacctccaga
     3001 actcactcct cgggatcgcc gatcacaacg tgcaggcgct gacggcgatc gaaatcgcgg
     3061 tgattccgcg ggaggcgatc ctgaagctgg ctgacgagcg cccggccgtg gcgagggcga
     3121 tgtggcacga caccctggtc gacgggtcgg tgcagcgcga atggacggcc aatatcggcc
     3181 gccgcgattc caagacgcgc atggctcacc tgctgtgcga gttcgcgctg cggcttgaaa
     3241 atgcgggcat cggccagttg tgcaattacg agctgccgat gacccaggag cagctcgccg
     3301 actgcctcgc gctcacgccc gtgcacgtca accggacgtt gaagctgctc gagaaggagg
     3361 cgctcatttc aaggacggtg cggtcggtga agatcgagga ttgggagaaa ctggcacggg
     3421 aaggcgactt caacaccacc tacctccacg tcgacctcca aggccgctcg atcgatccgg
     3481 tgccggcgct tcgtctcgcc tgatcggccg ttcaagcctt gacggctctc gccgcaggcc
     3541 acgcgaggag gaggtctgcc gcatagaccg gcatgttgcc ggcgaaatag agcgcgccct
     3601 ccggaaggcg gggtgcttta ggctgtatcg acattcgccg agctcgggct tctaagttcc
     3661 tcccctgcat ttgcagggga gggggaccat gcgaagcatg gtggaggggc ctttccgagt
     3721 cgaagaaccc ctccaccgcg cttcgcgcgg tccccctccc ccggaaatcc gggggaggac
     3781 ttaagaggga gcgaccttag ctgaatgtcg atacagccta gctccccctt cggccttcgg
     3841 acgaatgccg ccgcgagcag cgcgatcaag gccgccgccg cgaaagccgc gatggtctgg
     3901 tgcatcggca tcaggggcag tccgacgact gccgctcgat aacccagcgc acattcttgg
     3961 gcgaaactgg cagccgaacg atgtcgaccg ggctggtgag cgctgcgatc accccgcatc
     4021 ggcgccctgg agagacgaag cggacgaagg cgagcagctc gtccggttcc tcgacgacct
     4081 tgtcgatgac cactcgatag ccgcccgagg gcttcggccc cattgccgcc accaggatca
     4141 tctcgcgccg aaaatcgacc gcggcagcgg cggcgggtcg ccctggcgcg cggtgatccg
     4201 actccactga ccctgccact cggcgggtct ccggatcgcc atcatccgct cctggtagag
     4261 gccgctcatg tggcggaagg cggaagcggt ccgctcgtcg aagcggacga gcgggagccc
     4321 ggccggccgc tcctccgcgg gcacccggct ccagtcgtcg atccggcccg cgaggacgat
     4381 ccgagccgcc tcgccggtgc ggccggcaaa gccgcaggga aaggcggaga accgctcgcg
     4441 cccctggtgg cgcgccagca cgagggcgcc gccgggatcg gtgcagcgcg aatcgggcgc
     4501 cggctccgcc cacaaggcgc gcccggcgag gagcctgtcg agcgctcgcc cggcggcggc
     4561 atcgagggac tcgcggcgcc cgtctccgga gagccggacc cagccgccgg ccgtccgcac
     4621 cgccgccacg aaagccggac gctgctgccg gtacgagccg tctgcctgcc ggaccgtgta
     4681 gggaaaggag acgggcccgc ggaccagcag cgcagtccgc gccgcccgga cctgcgcgag
     4741 cgcggccggt cccagcgccg gctcagcctc ccgctcgaac tgggtccgcc atccgccccg
     4801 atcttgcgac tgcgccgccg ccgacggcgt gcccgacagc agggccagcg cgaagctcgc
     4861 caagatcgtt cggatcggcc gcattccttc ctccttaccc atcgccgtgg ctcgggcggc
     4921 aacatggcgg aaggaacctg aacgggatcg ggccgcgggg gtggctaggc gacgtcgctt
     4981 cggccttcga tctgaagagc gtcgctctcg gcttcctcga actcgccgcc gcgccccgcc
     5041 agcgcggccc gcaagtgccc caggtcgacc gctttctccc accgcgccac gacgatggtg
     5101 gcgaccgcgt tgccgatgaa gttggtgagc gaccggcatt cgctcatgaa ccggtcgacc
     5161 ccgaggatca gcgccattcc agccaccggc actgtcggga gcaccgacag ggtggcggcg
     5221 agcacgatga agcccgatcc ggtcactcca gccgcgccct tcgagctcag gatcgccacc
     5281 gccatcagga gcatctggtc ggcgaagccg agcggagtgt cggtcgcctg ggcgatgaac
     5341 aaggcggcga gcgacatgta gatgttggtg ccgtcgaggt tgaagctgta gcccgtcggg
     5401 acgacgaggc cgacgatcgg cttggggcag ccggcccgct cgagcttctc catcagcagc
     5461 ggcatcgcgc tctcggagct cgacgtgccc agcacgagca gcagctcgtc cttcagatag
     5521 cggatgagcg cgaagatcga gaagcccgcc gcccgggcga ccagcccgag cacgaccagg
     5581 acgaatatga gcgacgtggc gtagaaggtc gcgaccaggg tgatgagctg gcgcaacgta
     5641 tcgatgccgt gagccccgat cgtataggcc atcgccccga aggccccgat cggcgccgcg
     5701 tacatcaata tgtggacgat ccggaacatg accgcggtca gcacccgcat ccccccgagc
     5761 acccgctctc cgcgctcgcc gaggaagccg agcgcgatcc cgaacaggat cgacacgaac
     5821 agcacctgca ggatctggcc ggaggtgaag gcgctgaaga aggtgtccgg gatgatgtcg
     5881 agcaggaagg cggtaatgga ctggtcgtgc gccttgtcga cataggtcgc caccgccccc
     5941 tgatcgagcg aagccggatc gacgttcagc ccggcccccg gcctgacgac gttggccacg
     6001 atcagcccga cggcgagcgc cagcgtggag aaggtcagga aataggccat ggccttcgcc
     6061 ccgacccgcc cgaacgcctt gaggtccgac atccccgcga ttccggtggt gacggtaagg
     6121 aagatgaccg gcgtgatgat catcttcacc agccggatga aggcgtcgcc gagcggtttc
     6181 agcgaagcgc cgaaagcggg aaacagccag ccgacgagga tgcccaaggc aatggcgacg
     6241 agcacctgga agtagaggct gcggtagaag cgcctgggcc gggtggcggt catcgcgtct
     6301 gtctccttgc ggctctcacc gccgccgcat agcgcgggtc gagctggatg gcgcgctctt
     6361 cgtcggagcg ggcgcgccgg ctgtcccctc gctggcgcag caagaggctg cgatggtaat
     6421 agccccgggc ggactgaggc gcataggcta ccgcccggtc gagatcggcc agcgccccgt
     6481 cgagatcgcc caggccgcgc cgggccaggc cgcggttgag ataggcggcg gaggatcgcg
     6541 gggcgagcgc gatcgcccgg tcgaaggcgc ccacagcttt ttcgagctcg ccttcccagg
     6601 cccgcgcaag cccgtcggcg agctgggccg aagccttgcc ggaggcgcct ctcgcgaccg
     6661 aagcggcatc ccttcggcag gcgcgcgggt cgcgtttggg atcgtcgacc gtgcaggcat
     6721 tccagtcacc ccgccgaccc ccgccggttt cgaagaactc ctcgccgccg accgacgtga
     6781 tcgggaccgc cgagtccagg ttcggccgcc ggatccgcga tcccgtgacg acgatgtcgt
     6841 cgcccccgac ctccgtgacc ggcaccgaag acaccgtgtc cggccgccgg atccgggttc
     6901 cagcgaccac ggcctccccg ctttcggccg gaacgacatc ggcgagcgtg tcaccgcccg
     6961 gagcgggtac gggcatgtcg gctgtccccg ccggcggaag ttcggcgcgc ccgggcttcg
     7021 ccaagtccgg agccgcgtcc gctgtagacg gcggcgttcc gggatcgccg ggcgaattgg
     7081 cgatcgccgc cggatcgccc gattcgatgc gcctgtcggg gcggctgtcg cgagcctggt
     7141 ccggcagcga cgtccaggcc agcggcaagg cgatcagcgc gaccaggaag gcgcccgcga
     7201 gcgctcccac ctgggggcg
//
LOCUS       DASWNU010000290         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_115263, whole genome shotgun sequence.
ACCESSION   DASWNU010000290 DASWNU010000000
VERSION     DASWNU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115263"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VGB57_05715"
     CDS             complement(<1..742)
                     /locus_tag="VGB57_05715"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEY0130884.1"
                     /translation="MAFVELSGEQIRQLINTEQTYDAFVRAKAERERYRGAMTWKQVR
                     GREYLYRKRAGVWKSLGPRGADTELAYESFHSGRSAARERLASLDQVIRAQAPVNRAM
                     RLGRVPWPAARLLRRLENKRVLGHGISIVGTNALFAYERMAGAHFAGSEVTTTDIDLL
                     FDARDRLRLLAPNLQDGGLLGILRHVDDTFEMLAPGSFRAVNSAGYIVDMVKPMPRAP
                     AGTRERTRLGDDPDDMTAAEIEGLVWLQN"
     gene            918..2843
                     /locus_tag="VGB57_05720"
     CDS             918..2843
                     /locus_tag="VGB57_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0130885.1"
                     /translation="MADGTRFDTHVTAERFQLLINSVTDYAIYMLDSDGYVATWNPGA
                     RRFKGYEAEEIIGRHFSTFFTEEDRDAGVPAHALRTAAAEGRFEAEGWRLRKDGTRFW
                     VNAVLDPIRDDKGKLLGFAKITRDDTEKRQAALALQESERRFRTLVQGVKDYALYMLD
                     PDGVVSNWNTGAEAIKGYKADEIVGQHFSRFYTEEDRAAGEPRRALATAIAEGKYEKE
                     AWRLRKDGTRFYASVVIDPIHDENGTLLGFAKITRDITERRKANEELEEARAALFQSQ
                     KLQALGELTGGVAHDFNNLMTVIRGSAELLTRPGLGEEKRARYLQAILDTADRASTLT
                     SHLLAFGRRQALKPEVADLNLRLDAFAEMVGRTLGGQFEVRLDLAPSLPPVEVDLAQL
                     ETALLNAAFNARDAMPEGGILTLTTRHLAGEAGGEVMVEVADTGSGMSAEILDRAFEP
                     FFTTKPVGEGTGLGLSQIHGFAAQAGGRAEIRSVPGEGTAVRNILPATGKAFGRSTAA
                     VRAAKPPRGLGVLLVEDNDHVREFACHLLDELGYRVVTAASGEEALERLETEPVDILF
                     SDVVMPGLSGVELARLARERDPGLPVLLASGYSEEIVGAAGAEFEIVRKPYDLSGIEA
                     ALGKALEHAGARAESLS"
BASE COUNT          498 a          956 c          956 g          480 t
ORIGIN      
        1 tgttttgcag ccagacgagg ccttcaatct cggcggcggt catgtcgtcg gggtcgtcgc
       61 ccaatcgggt cctttcccgc gtccccgcag gagccctggg catgggtttg accatgtcta
      121 cgatgtatcc ggcggagttc accgcgcgaa agcttccggg ggcgagcatt tcgaacgtat
      181 cgtcgacatg ccgcaatatc cccagaaggc ccccatcctg caaattcgga gcgagcagcc
      241 tcaatcgatc gcgtgcgtca aacaggaggt cgatatcggt agtcgtcact tccgagccgg
      301 cgaaatgagc gcccgccatc cgctcatagg cgaacagggc atttgtaccg acgatgctaa
      361 tcccgtggcc aaggacccgc ttgttctcga gccgccggag cagccgcgca gcgggccacg
      421 gcacccttcc caggcgcatg gcccggttga cgggcgcctg ggcgcgaatg acctgatcca
      481 gcgaggccag ccgttcccga gctgcggaac ggccggaatg aaagctctca taggcaagct
      541 cggtgtcggc gccgcgcggg cccaggctct tccagacacc tgctcgtttg cgatagagat
      601 attcgcgccc acgcacctgc ttccatgtca tcgcgcctcg atagcgttcg cgttccgcct
      661 tcgccctcac gaaggcgtcg taggtttgct cggtgttgat cagctgccgg atttgttcgc
      721 ctgacaattc gacgaaggcc acgattatcc ccttttgaga attttctacg ggaaagggga
      781 taatcaggca aggatgtagc tgtggcccct tccaagcgga ctgaagcctg gacaagattt
      841 tgcctgttgc ggcgcggata ggagcggcgt agggccgctc cttcggccat gtggccgccc
      901 cttccgcagg tcgctggatg gctgacggta cccgcttcga tacccatgtc acggcagagc
      961 gctttcagct gctcatcaac tccgtcaccg attacgccat ctacatgctc gactcggacg
     1021 gctatgtcgc cacctggaac cccggcgccc ggcgcttcaa gggttatgag gcggaggaga
     1081 taatcggccg ccatttctcg accttcttca cggaggagga ccgggacgcg ggcgttccgg
     1141 cccatgccct caggaccgcc gcggccgagg ggcggttcga ggctgaaggc tggcggctgc
     1201 gcaaggacgg cacccggttc tgggtcaacg ccgtgctcga cccgatccgc gacgacaagg
     1261 gcaagctgct cggcttcgcc aagatcaccc gcgacgatac ggagaagcga caggccgctc
     1321 tcgccctcca ggagagcgag cgccgcttcc ggacgctcgt ccagggcgtc aaggactatg
     1381 ctttgtacat gctcgatccc gacggcgtgg tctccaactg gaacacgggc gcggaggcga
     1441 tcaagggcta caaggcggac gagatcgtcg gccagcattt ctcgcgcttc tacaccgagg
     1501 aggaccgggc ggcgggcgag ccccggcgcg cgctcgcgac ggcgatcgcc gagggcaaat
     1561 atgagaagga ggcctggcgg ctccggaagg acggcacccg cttctatgcg agcgtggtca
     1621 tcgatcccat ccacgacgag aacggcactt tgctcggctt cgccaagatc acccgcgaca
     1681 tcaccgagcg gcgcaaggcc aatgaagagc tggaggaagc gcgggcggcc ctgttccagt
     1741 cgcagaagct tcaggcgctg ggcgagctta ccggcggggt cgctcacgat ttcaacaatc
     1801 tgatgaccgt catccgcggc tccgccgagc ttctcacccg gcccggcctc ggcgaagaga
     1861 aaagggcgcg ctacctccag gcgatcctcg ataccgccga tcgcgcctcg accctgacca
     1921 gccatctgct cgctttcggc cggcggcagg cgctgaagcc cgaagtggcc gatctcaacc
     1981 tgcgcctcga cgccttcgcc gagatggtcg ggcggacgct cggcgggcag ttcgaggtca
     2041 ggctcgacct cgctccgtcc ctgcccccgg tcgaggtcga cctcgcccag ctcgagaccg
     2101 ccttgctcaa tgccgccttc aacgcccgcg atgcgatgcc ggagggcggc atcctgaccc
     2161 tgaccacccg ccacctcgcg ggcgaggccg gcggcgaggt catggtcgaa gtcgccgaca
     2221 ccggcagcgg gatgagcgcc gagatactcg accgcgcctt cgaacctttc ttcaccacca
     2281 agccggtcgg cgaggggacc ggcctcggcc tctcgcagat ccacggcttc gctgcgcagg
     2341 ccgggggccg cgccgagata aggtccgtgc cgggcgaggg tacggccgtt cggaacatcc
     2401 ttccggcgac cggaaaagcc ttcgggcgtt cgaccgccgc ggtccgagcg gccaagccgc
     2461 cgagggggct gggcgtgctc ctcgtcgagg acaacgacca tgttcgggag ttcgcctgcc
     2521 atctgctcga cgagctcggc taccgggtcg tcaccgcggc gtcgggggag gaggcgctgg
     2581 agcggctgga gacggagccg gtcgatattc tcttcagcga cgtggtcatg ccgggcctga
     2641 gcggagtcga gctggcccgg ctggcgcggg agcgggatcc gggcctgccg gtccttctcg
     2701 ccagcggcta cagcgaggag atcgtaggcg cggcgggcgc cgagttcgag atcgtccgca
     2761 agccctatga cctgagcggc atcgaagcgg cgctgggcaa ggcgctggag catgccgggg
     2821 cccgggcaga gagtctgtcc tgaaaagttg aagcaccagt tccgggaagc gatcccacct
     2881 cggcaggact
//
LOCUS       DASWNU010000291         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_62216, whole genome shotgun sequence.
ACCESSION   DASWNU010000291 DASWNU010000000
VERSION     DASWNU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62216"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VGB57_05725"
     CDS             complement(<1..692)
                     /locus_tag="VGB57_05725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0130886.1"
                     /translation="MVSHSGLITVMQRAARKAAPRIRRDFGEVEQLQVSRKGPADFVS
                     MADKRAEKTVMDELRHARPDWGFLMEEAGAVEGAPGKPRWVLDPIDGTSNFLHGIPHF
                     AISIAVQEAKPGGGWGEISQGLVYQPLTDESYWAEKGRGAWLNEGRLRVSSRRDLADA
                     LIATGVPYKGHGHLDRFQKILEAVSPEVAGIRRFGSAALDLAWVAAGRYDGFWEEDLQ
                     PWDVAAGILLVR"
     gene            complement(692..1255)
                     /gene="efp"
                     /locus_tag="VGB57_05730"
     CDS             complement(692..1255)
                     /gene="efp"
                     /locus_tag="VGB57_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0130887.1"
                     /translation="MKISGVDIRPGNIIEYEGGIWRAVKIQHTQPGKGGAYMQVEMKN
                     LIDGRKNNVRFRSAETVERVRLDTKDFQFLFRDGDMLTFMDKENYEQITLPADLLGDA
                     AAFLQDGMDVVMELWEERPISVQLPDTIEATIVEADAVVKGQTASSSYKPALLENGVR
                     VMVPPHIGAGTRIVVDVYEQTYIRRAD"
     gene            complement(1294..1908)
                     /locus_tag="VGB57_05735"
     CDS             complement(1294..1908)
                     /locus_tag="VGB57_05735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0130888.1"
                     /translation="MARPILLVAVTAIVTATLTSGVWMYALSEGPVDDRAPSASATPP
                     PAPQRPAVVIGPGGLAIPVAGIRPEQLSDTYTQSRAGGARAHNAIDIMAPRGTAVVAA
                     APGTVEKLYFSRGGGGISAYVRSPDRRWIYYYAHLDAYAPGLREGQVLRQGDPVGTVG
                     FTGNANPAGPHLHFAVHAMEQGETWHEGQAVNPYPLLAGSGARR"
     gene            1956..>2498
                     /locus_tag="VGB57_05740"
     CDS             1956..>2498
                     /locus_tag="VGB57_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0130889.1"
                     /translation="MAKRGEIRIGCSGWIYRHWRELFYPAKLPQRLWFEHYASTFDTV
                     ELNTSFYHLPKPDSFAKWRDQAPPGFRYAVKAPRFITHMKKLKDCSEPVEEFLRRARN
                     LGETIGPILYQLPPRWTCNTERLEEFVALLPADLAHVFEFREKSWLTLEVLELLDSRG
                     ISFCAHDMPDSATGRWASGPI"
BASE COUNT          393 a          848 c          837 g          420 t
ORIGIN      
        1 tcccgcacca gcaatatgcc ggcggcgacg tcccagggct ggagatcctc ttcccagaag
       61 ccgtcgtacc ggccggccgc gacccaggcg aggtcgagcg ccgccgaacc gaagcggcgg
      121 atcccggcca cttcgggcga caccgcctcc agtatcttct ggaagcgatc gagatggccg
      181 tggcccttgt aaggcacgcc ggtggcgatc agcgcgtcgg caaggtcgcg gcgcgaggag
      241 acgcgcagcc gcccctcgtt gagccacgca cccctgccct tctccgccca atagctctcg
      301 tcggtgagcg gctggtagac caggccctgg ctgatctccc cccagccgcc gccgggcttt
      361 gcctcctgga cggcgatcga gatcgcgaaa tgggggatgc cgtgaaggaa gttggaggtg
      421 ccgtcgatcg ggtcgagcac ccagcgcggc ttgccgggcg caccctcgac cgcgcccgct
      481 tcctccatca ggaagcccca gtccggccgg gcgtggcgga gctcgtccat caccgtcttt
      541 tctgcgcgct tgtcggccat cgagacgaag tcggccggcc ccttgcggct gacctgcaat
      601 tgctcgacct cgccgaagtc gcgccttatg cgcggcgccg ccttgcgggc cgcgcgctgc
      661 atgacggtga tgaggccgga atgggatacc atcaatcggc gcggcggatg taggtctgct
      721 cgtagacgtc gacgacgatc cgagtgccgg cgccgatatg cggcggcacc atgacgcgca
      781 cgccgttctc gagcaaagcc ggcttgtagc tcgacgaggc ggtctggccc ttcacgaccg
      841 cgtcggcttc gacaatggtc gcctcgatcg tgtcgggaag ctggaccgag atcggccgct
      901 cctcccagag ctccatcacc acgtccatcc cgtcctggag gaaggcggcg gcatcaccga
      961 gcaggtccgc cggcagggtg atctgctcgt aattctcctt gtccatgaag gtcagcatgt
     1021 cgccgtcgcg gaacaggaac tggaaatcct tggtgtcgag ccggacgcgc tccaccgttt
     1081 ccgccgagcg gaagcggaca ttgttcttgc ggccgtcgat gaggttcttc atctcgacct
     1141 gcatgtaggc gccgcccttg ccgggctggg tgtgctggat cttcaccgcg cgccagatgc
     1201 cgccttcata ttcgatgatg ttgccgggac gaatgtccac gccgctgatt ttcatggaat
     1261 gctcgcgaaa gtgggaaaag ggtcgccggg cctttagcgg cgggcgccgc ttccggcaag
     1321 gagcggatag ggattgacgg cctggccctc gtgccaggtt tcgccctgct ccatcgcatg
     1381 gacggcgaaa tgcaaatgcg ggccggcggg attggcgttt ccagtgaagc cgacggtgcc
     1441 gaccgggtca ccctggcgga ggacttgccc ttcccggagc cccggggcgt aggcgtcgag
     1501 atgggcgtaa taatagatcc agcggcgatc gggcgaacgg acataggccg agatgccgcc
     1561 gccgcccctg ctgaaataga gcttctccac ggtgccgggc gcggccgcga ccacggcggt
     1621 cccgcggggc gccatgatat cgatcgcatt gtgggcgcgg gcgccgccgg cgcgcgactg
     1681 ggtgtaggtg tcggacagct gttcgggacg gatgccggcg accgggatgg cgaggccgcc
     1741 ggggccgatg acgactgcgg gtctctgcgg ggccggcgga ggcgtggccg aggcggaggg
     1801 cgcccggtcg tccaccggcc cctcgctcag cgcgtacatc cacacgccgc tggtcagcgt
     1861 cgcggtgacg atcgcagtca cggctacgag caggatcgga cgcgccatgc ggctccaacg
     1921 caacttattt cccacccgtt ccgttcgggg cggcgatggc aaagcgcggc gagatcagga
     1981 tcggctgctc gggctggatc taccggcact ggcgcgagct cttctatcct gcgaagctgc
     2041 cgcagcgcct ttggttcgaa cattatgcgt cgaccttcga tacggtcgag ctcaacacca
     2101 gcttctacca cctccccaag cccgacagct tcgccaaatg gcgcgaccag gcgccgccgg
     2161 gcttccgcta tgcggtcaag gcgccccgct tcattaccca catgaagaag ctcaaggact
     2221 gttccgagcc ggtcgaggag ttcctccgcc gggcccgcaa cctcggcgag acgatcggcc
     2281 cgatcctcta ccagctgccg ccacgctgga catgcaacac ggagcggctg gaggagttcg
     2341 tcgccctcct gcccgccgac ctcgcccacg tcttcgagtt ccgcgagaag agctggctga
     2401 ccctggaggt gctcgaactg ctcgactcgc gcggcatctc cttctgcgcc cacgacatgc
     2461 cggactcggc gaccgggcgc tgggcctccg ggccgatc
//
LOCUS       DASWNU010000292         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_89015, whole genome shotgun sequence.
ACCESSION   DASWNU010000292 DASWNU010000000
VERSION     DASWNU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89015"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1314
                     /locus_tag="VGB57_05745"
     CDS             358..1314
                     /locus_tag="VGB57_05745"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY0130890.1"
                     /translation="MPGSFPHRHLLGIEDLSVGDILFILAEAEQWVAFNRGPRKQDFR
                     LAGLTQVNAFFENSTRTLLSFEIAGKRLGAQVVNLHAAQSSLSKGEGLDDTIRTIGAM
                     RPDLLVVRHKEAGMARDIARHTDCAIVNAGDGENEHPTQALLDALTIQRRKGRIEGLT
                     IAICGDVRHSRVARSNMRSLALLGARVRVVGPPALLPDPDELGSTVSFTNLEQGIADA
                     DVIMMLRVQRERISPELFDGEPDLFGAPGDYRTLYGLTRERLDRAAPRALVMHPGPMN
                     RGVEIDGDVADDPERSAIFEQVEMGVAVRMAVLDILTRDKRG"
     gene            complement(1330..>2015)
                     /locus_tag="VGB57_05750"
     CDS             complement(1330..>2015)
                     /locus_tag="VGB57_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0130891.1"
                     /translation="PAAPSGTAFAAAEPLPEPAPETVTSTAPALALAEIAPEPAPVLA
                     QAEPAPAPEPVRIAGGELPSWVSERAAAEAAAPAQAEETKPLYAEAVQALVTPQPSVS
                     APQRSIEPLAPRFEAPRRQPRAVSAGPGRFAVQLGAYSSSSSVEKAWAQMLRRFGFAD
                     LTPLSTTVSMPGRGTLHRLSVAGFASREAAGRTCRSIQAKGGACFVRTVAGDAPVRWA
                     GRYATGRRG"
BASE COUNT          332 a          656 c          705 g          322 t
ORIGIN      
        1 ccttgccgaa agcgcgatcg atctgcgcca gcgcggcctc gagggccttc tgcctgtcca
       61 tattgtccga gtttcccacc accttgagtg aagccgccat ggcccgcatc ccctattgcc
      121 tctgcgccgc ccattcaacg cgattgttgc aatgtacgcg atccgttcca tgagaacaag
      181 aggaaaacgc ctagccatcc tgggctttgc cgaggcttat tcaaaaagcg cttgaaacgg
      241 cagcggacgt tttacggaac ccgccccggc cgttccgccg gccgccggcc acattcgaca
      301 cggtcctgct tcgggcggct tgccggtgag gcacacgcgc cttatcgcgg ctatccgatg
      361 cccggctcct tcccgcaccg ccatctgctc gggatcgaag atctctcggt cggcgatatc
      421 cttttcatcc ttgccgaggc cgagcagtgg gtggctttca acagaggtcc tcgaaagcag
      481 gattttcgct tggcgggcct cacccaggtc aacgccttct tcgagaattc cacccgaact
      541 ttgctgtcct tcgagatcgc cgggaagagg ctgggcgccc aggtcgtcaa cctgcacgcg
      601 gcccagtcga gcctcagcaa gggcgaaggg ctcgacgata cgatccggac catcggagcg
      661 atgcggcccg atctgctggt ggtaaggcac aaggaggccg gtatggcgcg ggacattgcc
      721 cgccataccg attgcgcgat cgtcaatgcg ggcgacgggg agaacgagca tcctactcag
      781 gctctgctcg atgccctcac cattcagcgg cgcaaagggc ggatcgaagg gctgactata
      841 gcgatttgcg gcgatgtgcg gcacagccgg gtggctcgct caaacatgcg ctccctggcc
      901 ctgctcggtg cgagggttcg agtcgtcggc cccccggcgc ttctccccga tcccgacgaa
      961 ctgggttcga cggtctcgtt cacgaatctt gaacaaggca tagccgatgc cgacgtcatc
     1021 atgatgttgc gcgtccagcg cgagcggata agccccgagc tctttgacgg ggaacccgac
     1081 ctgttcgggg cccccggaga ttatcggacg ctctacggcc tcactcgcga acgcctcgac
     1141 cgggcggcac cgcgcgcgtt ggtcatgcat cctggaccca tgaaccgtgg cgtcgagatt
     1201 gacggcgatg tggcggacga tcccgagcgg tccgccattt tcgagcaggt cgaaatgggt
     1261 gtcgcggtca gaatggccgt tctggacatc ctgacccggg acaaaagagg ataggccctc
     1321 gtccggaaat cagccgcggc ggccagtggc gtagcggccg gcccagcgga cgggagcgtc
     1381 gccggcgacg gttcggacga agcaggcgcc gcccttggcc tggatcgagc ggcaggtgcg
     1441 gcccgccgcc tcgcggcttg cgaagccggc gaccgagagc cggtgaaggg tgccccggcc
     1501 gggcatggag accgtggtgc tgagcggagt gagatcggcg aagccgaagc gcctgagcat
     1561 ctgcgcccac gccttttcca ccgaggagga ggaggaataa gcgccgagct ggacggcgaa
     1621 gcggccgggg ccggccgaaa cggcgcgggg ctggcggcgc ggcgcctcga agcggggcgc
     1681 gagcggctcg atcgagcgct gcggagcgga caccgaaggc tgcggagtga cgagcgcctg
     1741 cacggcctcg gcgtaaagcg gcttcgtctc ctccgcctgg gccggggcgg cggcctcggc
     1801 ggcggcgcgc tcgctgaccc atgaagggag ctccccgccg gcgatccgga ccggctcggg
     1861 ggccggcgcc ggttccgcct gggcgagaac cggagcgggc tcgggagcga tttcggccag
     1921 agcgagggcg ggcgcggtgg aggtcaccgt ttccggcgcc ggctccggca gcggctcggc
     1981 ggccgcgaat gcggtgccgg agggcgccgc agggg
//
LOCUS       DASWNU010000293         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_140286, whole genome shotgun sequence.
ACCESSION   DASWNU010000293 DASWNU010000000
VERSION     DASWNU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140286"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VGB57_05755"
     CDS             complement(<1..190)
                     /locus_tag="VGB57_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0130892.1"
                     /translation="MSIFQLIHQKVHGKAVFDRRIEVLTRTLADFIPANARVLDIGCG
                     SGTLARKIMALRPDISIEG"
     gene            complement(196..1383)
                     /locus_tag="VGB57_05760"
     CDS             complement(196..1383)
                     /locus_tag="VGB57_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0130893.1"
                     /translation="MGFVPQAGAAGLELTIVIPCLNEAETLAQVIDRARRFLHAYEVS
                     GEVVVADNGSTDGSTAIAIGAGARVVPVAERGYGAALRAGIEAARGTYVAMADADCSY
                     DFMDLMPFLERLRSGTELVVGNRFLGGIGKGAMPPLHRYLGNPVLSLAGRVFFRAPIG
                     DFHCGLRAFRRDSILELGLSSPGMEYASEMIVKAQLAGVSMSEVPATLAKDGRSRPPH
                     LRSWRDGWRHLKFLLLFAPRSLYLYPGLGLFALSFAVFLGLLPGDVTVGSVRFGVHSL
                     LFAGAGMLIGAQMMSFGLLAGLFGVRERYWSAGRGLERARALLTIDKGSLIGGAMMLA
                     GLGGAVVALVSWAGAGFSDMNPDMLMRISIPSVLSAGIGLQLMLTAFLLELLSHPARH
                     PQR"
BASE COUNT          299 a          527 c          629 g          254 t
ORIGIN      
        1 tcccctcgat gctgatgtcg ggacggagcg ccatgatctt cctggcgagc gttccgctgc
       61 cgcagccgat gtcgagcacc cgggcattgg cggggatgaa gtcggcgagg gtgcgggtga
      121 gcacttcgat ccggcggtcg aacaccgcct tgccgtgcac cttctggtgg ataagctgaa
      181 agatgctcat ggcgattacc tttgcggatg gcgggcgggg tgggacagaa gctcgagcag
      241 gaaggcggtg agcatgagct gcagtccgat gccggcggag aggacggacg ggatggagat
      301 gcgcatcagc atgtccgggt tcatgtcgct gaagccggcc ccggcccagg agaccagggc
      361 gacgaccgct ccgccgaggc cggcgagcat catcgccccg ccgatcaggg agcccttgtc
      421 gatggtgagc agggcgcggg cccgctcgag gccgcggccg gcggaccagt agcgctcgcg
      481 gacaccgaac aggccggcga gcaatccgaa gctcatcatc tgggcgccga tcagcatacc
      541 ggcgccggcg aagagcaggc tgtggacccc gaagcggacg ctcccgacgg tgacgtcgcc
      601 cgggagcagg ccgaggaaca ccgcgaagct cagcgcgaac agcccgaggc ccggatagag
      661 gtagagcgag cgcggcgcga agaggagcag gaacttgagg tggcgccagc cgtcgcgcca
      721 gctgcgcaga tggggcgggc gcgagcggcc gtccttggcc agcgtggccg gcacctccga
      781 catggagacc ccggcgagct gcgccttgac gatcatctcc gaggcatatt ccattccggg
      841 cgacgacagg ccgagctcga ggatcgaatc gcggcggaag gcgcgaagcc cgcaatggaa
      901 gtcgccgatc ggggcgcgga agaagacccg tccggcgagg ctgaggaccg ggttgccgag
      961 atagcggtgg aggggcggca tggcgccctt gccgatgccg ccgaggaagc ggttgccgac
     1021 gacaagctcg gtgccggagc gaagccgctc gaggaagggc atcaggtcca tgaaatcgta
     1081 gctgcagtcg gcgtcggcca ttgcgacata ggtgccgcgg gcggcctcga ttccggcgcg
     1141 aagggcggcg ccgtagccgc gctcggcgac cggaacgacc cgggcgccgg cgccgatggc
     1201 gatggcggtg gagccgtcgg tggagccgtt gtcggcgacg acgacctcgc cggaaacctc
     1261 ataagcatgg agaaagcggc gcgcgcggtc gatcacctgg gccagcgtct cggcctcgtt
     1321 gaggcaggga atgacgatgg tgagctcgag cccggcggcg ccggcctggg ggacgaaacc
     1381 caccggcgtc tcgctccgcg cctttttcct gaccccctgc acttgcgatt tctccctcgt
     1441 tcttcggttc ttgggagggg tttggcagcc catggttaat tttctgttga tggggaattt
     1501 gtcctcccca tcgccgcgcg atggggaggg ggaccaagga accctggtgg aggggtaact
     1561 ggctctacct cagggggcct cgcttgaaat aacatcgcgc ggcgccatct acccctccac
     1621 cacacttcgt gtggtccccc tccccatgca gcgcatgggg aggacaaatc ggaacctgcc
     1681 catgtcgcgc cgcattgatc tgcttatgg
//
LOCUS       DASWNU010000294         6351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_140366, whole genome shotgun sequence.
ACCESSION   DASWNU010000294 DASWNU010000000
VERSION     DASWNU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140366"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="crtI"
                     /locus_tag="VGB57_05765"
     CDS             <1..635
                     /gene="crtI"
                     /locus_tag="VGB57_05765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEY0130894.1"
                     /translation="MLGHARGRQAAARLRRKRFSPSLFVVHFSTRGEWPDTPHHSILF
                     GPRYKELLGEIYRGSTLPDDPSIYLHHPTATDPAMAPAGKSTFYALAPVPHLGKAPLD
                     WETEGSAYAARILQILEERALPGLSNRLEILFHFGPAEFESDLNSHLGSAFSLEPLLT
                     QSAWFRVHNRDDEIPNLYFVGAGTHPGAGIPGVVGSAKATAGLVIEDLLG"
     gene            632..1504
                     /locus_tag="VGB57_05770"
     CDS             632..1504
                     /locus_tag="VGB57_05770"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY0130895.1"
                     /translation="MNRSKLVAAAGESIRLGSKSFHMASRLFDRRTRERAWLLYCWCR
                     HCDDICDGQALGFRTERAQGNVEAVEVLTGQVLAGETIGLLPFDALGALLAECPVPHR
                     YILDHLQGFTLDADGWRPRDEADLLRYCYHVAGAVGCMMAVAMGVEPDEKEILARASD
                     LGIAFQLSNIARDLRDDHEAGRCYLPAEWIELHGLDRRDPLRPDRREALVAVAGRLTD
                     LALRYEASAHAGVAKLPFRARWAVLAAARVYGAIGRRVASLGASAWETRVTVPRREKL
                     SFLLPTLVEAIRTR"
     gene            1509..2732
                     /locus_tag="VGB57_05775"
     CDS             1509..2732
                     /locus_tag="VGB57_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEY0130896.1"
                     /translation="MAHFAFVAPPLRGHYRPLSHLAAELIARGHRATFLHHEDAAPLV
                     EAEGAGFEALGRGAPPVASWTDPMARIRGLIGLGGTLDGMIRFTDMFCGEAPSALRRI
                     GADAMIVDQLEAGGGLVAEHLGLPFASVAVTVPINREPGVPPPYVGWRHDPSERAVRR
                     NVGGWRISDRLMRRVGDGIERNAARLGLPPRRRLDDCLSPRLQVSQMVRSLDFPRSRL
                     PADFHYTGPFRRAAPDSFELPPGDGRPLVYCTLGTLQGSRAGIFRKVARACAELDLRL
                     LISQGNRGRLKPGDLPGNPLVYDWVPQEAVLARSDLMVCHGGMNTVLEPLAAGLPMVV
                     MPLAFEQSAIAARLEQAGVARKLGWRTSAHRLAEAIREVRSPVFRERAHAVRLEMDEA
                     GGVGRAADLIEAALS"
     gene            complement(2710..3453)
                     /locus_tag="VGB57_05780"
     CDS             complement(2710..3453)
                     /locus_tag="VGB57_05780"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0130897.1"
                     /translation="MTSLTASRARQTAIGLALAAAIAGAWAALHIGSVFFLPLQGAGL
                     AAAPPLVLAICWLNVGLFIVAHDAMHGSLAPGRPGVNLWAGRLALALYAGFSFDRLRP
                     KHFEHHRSPGTAADPDFSADSPDAFWPWYRAFFRQYFGWRELAVLTFLVAAYTLLLGA
                     PYPNLLLFWALPAILSSLQLFLFGTFLPHRAGAEDFGDEHRARSSDYGGLASLLTCFH
                     FGYHHEHHRSPATPWWRLPAERLSSRRPR"
     gene            complement(3450..4031)
                     /locus_tag="VGB57_05785"
     CDS             complement(3450..4031)
                     /locus_tag="VGB57_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0130898.1"
                     /translation="MAGGWIVALGIVFGTVVAMEAVAWAVHRYVMHGWGWGWHRSHHE
                     PSEGRFETNDLYALVFAGISLLFFTLLNRLWPPLWWVGVGTLVYGLLYTLLHDGLIHR
                     RLGFGLSPRSGYLKRLVQAHRLHHAVRGRDGAVSFGFLYAPPARHLRRRLREQEAGPS
                     SRPTSSRRRPGSMNTDRAISESVLFMDPGSSPG"
     gene            complement(4108..4341)
                     /locus_tag="VGB57_05790"
     CDS             complement(4108..4341)
                     /locus_tag="VGB57_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130899.1"
                     /translation="MAINDPDGAGSDPTRGTGPDKATGMRPPGLDAPPHAQEGEPSGS
                     GAADPARGGCLKLGWGCLPVLLVAAFIPAGLIF"
     gene            complement(4328..4768)
                     /locus_tag="VGB57_05795"
     CDS             complement(4328..4768)
                     /locus_tag="VGB57_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEY0130900.1"
                     /translation="MDDDRVREFETALWIGGEDVYRRSIDPDCLMVVPAAPFLMSGSE
                     AIEAVSKTPRWESVEFDDLRIRRPQEGLIVLAYAVRAGRGGQAYRAHCTSTYRRVEHE
                     HWQVVQHQQTFAPVAAANVQSGSDAMERVQKDASEDRESEHGYQ"
     gene            complement(4761..5825)
                     /locus_tag="VGB57_05800"
     CDS             complement(4761..5825)
                     /locus_tag="VGB57_05800"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0130901.1"
                     /translation="MRPRLKPIDRQTIVITGATSGNGLATAREAARRGAAVVLAARNG
                     EALEAVRGDLERTGGRVAVCAADVSVEADVERIAATAIESFGGFDSWVNDAAAATYGA
                     MEQVPMEDHRRIFDVNYHGLLMGSLVAARHLRSRGGGAIVNLGSVLSDRAMIYQGPYS
                     ATKAAVQAATDALRMELERDGAGISVTLIKPGSINTPYPEHARNYMDSPPRLPPMLYD
                     PELVAEAIIFACETPRRQLYVGGSGYLISLAGRLAPRATDLAMEAFGVAAQQKPGDPG
                     DPGKRDNLYAPRADGESHGEQDVFVRRTSLFLQAQTQPLLLPFVAAAAGAELAGGLVR
                     NFLHRPGRKRPASRNGARHG"
     gene            6122..6307
                     /locus_tag="VGB57_05805"
     CDS             6122..6307
                     /locus_tag="VGB57_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130902.1"
                     /translation="MFEEENLNYHSERAQRELHLGLTASLMSVARSHLELSSLHMKKV
                     LEMRGPGRQARPQCIVD"
BASE COUNT          987 a         2176 c         2204 g          984 t
ORIGIN      
        1 acatgctggg ccacgcgcga ggccgccagg cggccgccag gcttcgccgc aagcgcttct
       61 cgccgtcgct gttcgtcgtc catttctcga cccgcggcga atggccggac actccccatc
      121 acagcatcct gttcgggccg cgctacaagg agctgctcgg cgaaatctat cgcggcagca
      181 cgcttccgga cgacccgtcc atctacctcc atcatccaac cgcgaccgat cccgccatgg
      241 cgcccgcggg caaatcgacc ttctacgcgc tcgcccccgt gccgcatctc ggcaaagccc
      301 cgctcgactg ggaaacggag gggtccgctt atgcggcgcg gatcctgcag atcctggagg
      361 agcgggctct tcccgggctt tcgaaccggc ttgaaatcct cttccacttc ggccccgcgg
      421 agttcgagag cgacctcaac tcgcacctcg gttccgcttt cagcctcgag cctttgctca
      481 cccagagcgc ctggttccgg gtgcacaatc gcgacgacga gatccccaat ctctatttcg
      541 tcggcgccgg gactcatccc ggcgccggaa tccccggggt cgtcggaagc gccaaggcca
      601 ccgccggact cgtcatcgag gatctcctcg gatgaaccgg tcgaagctcg tcgccgcggc
      661 cggggagtcg atccggctgg gctcgaaatc cttccacatg gcgagccgtc tgttcgaccg
      721 gaggactcgg gagcgcgcct ggctcctcta ttgctggtgc cgccattgcg acgatatctg
      781 cgacggccag gcgctgggct tccggacgga gcgggcgcag ggaaatgtcg aggctgtcga
      841 ggtccttaca ggccaggtcc tggccggcga gacgatcggg cttctgcctt tcgacgccct
      901 cggcgccctg ctcgcggaat gtccggtccc gcaccgctac atcctcgatc atctccaggg
      961 tttcaccctc gacgccgacg gctggcggcc tcgcgacgag gcggatctgc tgcgttattg
     1021 ctatcacgtc gcgggcgcgg tcggctgcat gatggcggtc gcgatgggcg tggagccgga
     1081 cgagaaggag attctggccc gggcatcgga tctcggcatc gccttccagc tttccaacat
     1141 cgcccgcgac ctcagggacg atcacgaggc ggggcgctgc tacctgccgg ccgaatggat
     1201 cgagcttcac ggcctcgacc gccgcgaccc gttgcggccg gaccgaaggg aggcgctcgt
     1261 cgccgtcgcc ggccggctca ccgatctcgc cttgcgctac gaggcttcgg cgcatgcggg
     1321 ggtggcgaag ctgccgttcc gggcgcgctg ggcggttctg gcggcggcgc gggtctatgg
     1381 agccatcggc cgccgggtgg cgtcgctcgg cgcctcggcc tgggagacga gggtgacggt
     1441 gccgcggcgg gagaagctgt ccttccttct gccgacattg gtcgaagcga tccggacgcg
     1501 ctgagccgat ggcgcatttc gcgttcgtgg cgccgccgct gcgcggtcat taccgcccgc
     1561 tttcccacct cgccgccgag ctgatcgccc gcggccaccg cgcgaccttc ctgcaccatg
     1621 aggatgcggc gccgctggtc gaggcggaag gcgccggctt cgaggcgctc ggccgaggcg
     1681 ccccgccggt cgcaagctgg accgatccga tggctcggat ccgcggactg atcggcctcg
     1741 gcggcaccct ggacgggatg atccggttca ccgacatgtt ctgcggcgag gcgccgtctg
     1801 cgcttcgcag gatcggagcg gacgcgatga tcgtcgacca gctggaggcg ggcggcggtc
     1861 tcgtcgccga gcatctcggc cttcctttcg cgagcgtcgc cgtgaccgtg ccgatcaatc
     1921 gcgagccggg cgttccgccg ccttatgtcg gctggcgcca cgatccgagc gagcgggcgg
     1981 tcaggcgcaa tgtcggcggc tggcggattt ccgaccggct gatgcgccgc gtcggcgacg
     2041 ggatcgagcg caatgcggcc cggctcggcc tgccgccccg gcgacgcctc gacgattgcc
     2101 tgtcgccgcg cctccaggtc tcccagatgg ttcgaagcct ggatttcccg cgaagccgcc
     2161 tgccggcgga cttccattat accgggcctt tccgccgggc cgcgccggac tcattcgagc
     2221 ttccgccggg ggacggccgg ccgctcgtct attgcacgct cggaaccctt caggggtcgc
     2281 gcgccggcat tttccggaag gtggcccgag cctgcgcgga gctcgatctg aggcttctga
     2341 taagccaggg gaatcgcggc cggctgaagc cgggagacct ccccggcaat cccctggtct
     2401 acgactgggt gccgcaggaa gcggtgctcg cgcggtccga cctcatggtc tgccacggcg
     2461 gcatgaacac cgtgcttgag ccgctcgccg cgggattgcc gatggtcgtc atgccgctcg
     2521 cgttcgagca atcggcgata gcggcccggc tcgagcaggc cggcgtcgcc cggaagctgg
     2581 gctggcggac gtccgcgcac cggctggccg aggcgatccg cgaggtgaga agccccgtct
     2641 tccgcgagcg cgcccatgcg gttcggctcg aaatggacga ggccgggggc gtgggccggg
     2701 ccgccgacct catcgaggcc gccttgagct gagccgctcg gcgggaagcc gccaccaggg
     2761 cgtcgcgggc gaccggtgat gctcgtgatg atagccgaaa tggaagcagg tcaggagcga
     2821 ggcaagcccg ccatagtcgc tgctcctggc ccggtgctcg tcgccgaaat cctccgctcc
     2881 ggcccggtgc gggaggaagg tgccgaacag gaagagctga agcgacgaga ggatcgccgg
     2941 aagcgcccag aagagcagca gattcgggta gggcgctccg agcagcaggg tgtaggcggc
     3001 gaccagaaag gtcagcaccg ccagctcgcg ccagccgaaa tattgccgga agaaggcccg
     3061 ataccagggc cagaaggcgt cagggctatc ggcgctgaag tcgggatcgg cggcggtgcc
     3121 ggggcttcga tgatgctcga aatgcttggg ccgaagccgg tcgaacgaga agccggcgta
     3181 gagagccagc gccagccggc ccgcccagag gttcaccccc ggccggcccg gcgccagcga
     3241 gccgtgcatg gcgtcgtggg cgacgatgaa gaggccgacg ttcagccagc agatggcgag
     3301 gaccagcggc ggggcggccg cgagccctgc gccttgcagc ggcaggaaga agacggaacc
     3361 gatatggagc gccgcccagg cgccggcgat cgccgcggcg agggcaagcc ctatcgccgt
     3421 ctggcgggcg cggctcgcgg tcaagctcgt catcccgggc ttgatccggg atccatgaac
     3481 aggacggatt ccgatattgc tcgatccgtg ttcatggatc ccggccttcg ccgggatgac
     3541 gtggggcgcg acgacggacc agcctcctgc tcgcgcagcc ggcgcctcag atgtcgtgcc
     3601 ggcggcgcgt agaggaagcc gaacgacacg gcgccgtccc gcccgcgcac cgcgtgatgg
     3661 agccggtgag cctggacgag ccgcttcaga tagccgctcc tcgggctgag cccgaagccg
     3721 aggcggcgat ggatgagccc gtcgtggagc aaggtgtaga ggaggccgta gacgagcgtt
     3781 ccaacgccga cccaccacag cggcggccac agccggttca gcagagtaaa gaagagcagg
     3841 ctgatcccgg cgaagacgag ggcgtagagg tcgttggtct cgaaccgccc ctcgctcggc
     3901 tcgtggtggg accggtgcca gccccagccc cagccgtgca tgacgtaccg atgcaccgcc
     3961 caggccaccg cctccatggc gacgaccgtc ccgaagacga tgccgagcgc gacgatccag
     4021 cctcctgcca tgagggcgag ataggaccgg ccctcggccg cgtccacgaa atggcgcccc
     4081 gtacggtaca aagcaggtag ggctgggcta gaagatcagc cccgcgggga tgaacgccgc
     4141 gacgagaagt acgggaaggc agccccagcc cagcttcagg caaccgcccc gggccggatc
     4201 ggccgctccc gagccggagg gctcgccttc ctgcgcatga ggcggcgcgt ccaggccggg
     4261 cggccgcatc ccggtggcct tgtcgggccc ggttccgcgc gtggggtccg agcccgctcc
     4321 gtccggatca ttgatagcca tgctcgctct cccggtcctc ggaggcatcc ttctgcacac
     4381 gttccatcgc gtcggatccg ctttggacgt tcgcggccgc aaccggagcg aaggtttgct
     4441 ggtgctggac gacctgccaa tgctcatgct cgacgcgccg gtaggtcgag gtgcaatggg
     4501 cgcggtaggc ctggccgccg cgcccggcgc gaaccgcgta ggccagcacg atcagccctt
     4561 cctggggccg ccggatcctg agatcgtcga actcgaccga ctcccaccgc ggagtcttcg
     4621 acaccgcctc gatggcctcg ctcccgctca tcaggaacgg agcggccggc accaccatca
     4681 ggcagtccgg gtcgatcgac cggcggtaga catcctcccc gccgatccag agcgccgtct
     4741 cgaattccct gacccgatcg tcatccatgc cttgctccgt tcctcgaggc cgggcgcttc
     4801 ctgccgggcc gatgcagaaa gttgcggacc aggccgccgg cgagctcggc gcccgccgcg
     4861 gcggcgacga agggcagcag cagcggctgg gtctgggcct ggaggaacag gctggtgcgg
     4921 cgaacgaaga cgtcctgctc gccgtggctc tcgccgtccg ctctgggcgc gtagagattg
     4981 tcgcgcttgc ccggatcgcc ggggtcgccg ggcttctgct gggccgccac tccgaaggcc
     5041 tccatggcga ggtccgtggc ccgcggcgcc agccggccgg cgagcgagat cagatagccg
     5101 ctgccgccga cgtagagctg ccggcgggga gtctcgcagg cgaagattat ggcttcggcc
     5161 accagctcgg gatcgtagag catgggcggc aggcgcggcg gcgaatccat gtagttgcgg
     5221 gcatgctccg gatagggcgt gttgatggag ccgggcttga tcagggtgac cgagatgccg
     5281 gcgccatcgc gctccagctc catgcgcagg gcgtccgtcg ccgcctgcac cgccgccttg
     5341 gtcgccgaat agggaccctg gtagatcatc gcccggtccg aaagcacaga accgagattg
     5401 acgatggcgc cgccgccgcg ggagcgaaga tggcgcgccg cgacgagcga tcccatcaga
     5461 aggccgtgat aattgacgtc gaaaatgcgc cggtgatcct ccatcggcac ctgctccatc
     5521 gccccgtagg tcgcggccgc cgcgtcgttg acccagctgt cgaagccccc gaaggattcg
     5581 atcgcggtcg cggcgatacg ctccacgtcc gcctcgacgg agacgtcggc ggcgcaaacg
     5641 gccacccgcc cgcctgtccg ctccagatcg cctctcaccg cctccagcgc ctcaccattg
     5701 cgggcggcga ggacaacggc ggcgccgcgc ctcgccgctt cccgggcggt cgccagaccg
     5761 ttgccgctgg tggcgccggt gatgacgatc gtctgacgat cgatgggttt aaggcgtggc
     5821 cgcatctcgt gcctttcggt tcgcaagggg ccaacccgcg aagtagcgaa gcgttgctac
     5881 ctgctttgga ccacctggaa cttttcggcc aagcctcggc gcgggcgaac cgccggtcgc
     5941 ggagcactga tcgacgatcg gaagatggtg ggtgagctca gagcctgatc gttccgccgg
     6001 agcggccggg cggcggcgcc aacctggatc agccggattt tctcccggcg cttaacgccg
     6061 gtcaatgtgc gagacaccgt ttcttgccat ttggccttaa gccgctggca aggaacagga
     6121 aatgtttgaa gaagaaaatc tgaactatca ttccgagcgg gcgcagcggg agcttcatct
     6181 gggcctcacc gcgtcgttga tgtcggtcgc cagatcgcat ctcgagctgt cgtcgctgca
     6241 tatgaaaaag gtgctcgaga tgcgcggccc gggccgccag gcgcgcccgc aatgcatcgt
     6301 cgactgaagc cacgccgaag cccgacccgg gacccgtgac attcggccta t
//
LOCUS       DASWNU010000295         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_165743, whole genome shotgun sequence.
ACCESSION   DASWNU010000295 DASWNU010000000
VERSION     DASWNU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165743"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VGB57_05810"
     CDS             complement(<1..1117)
                     /locus_tag="VGB57_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0130903.1"
                     /translation="MSRLIVISNRVSKPKGEGEGSQGGLAVALSAALRDHRGLWFGWS
                     GETAEQFTGHIDFDRHDGVTTATIDLEEQDVDEYYNGYANRTLWPLFHYRIDLAEYDR
                     SFAGGYERVNERFAETVQPLIEPDDLIWVHDYHLIPLGQQLRDRGVRNRIGFFLHIPW
                     PPARLMVSLPFHEGLVLSMLAYDVIGFQTEEWLESFRHYVERELGGHCDEDGTIHVGE
                     RSVHAGAYPIGIDYSEFAKAAASPRAQAAFERMRVSAAGRRTIIGVDRLDYSKGLTER
                     FNGYRRFLEQHEQCRGELFLLQIAPPSRGEVATYEVIRERLDELSGRINGDFAEVDWV
                     PIRYVNQGYAREDLGGFYRASQIALITPLRDGMNLVAK"
     gene            complement(1239..3029)
                     /locus_tag="VGB57_05815"
     CDS             complement(1239..3029)
                     /locus_tag="VGB57_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0130904.1"
                     /translation="MADLSLWPIGNCQVSALVDREAGFVWGCAPRVDGDPLFCSLLGP
                     KGESDLPRGEWRIAVDNQVSAEQSYLRNTAILTTRLTDSDGAVAEIFDFCPRFERSGR
                     MYRPVAFARIVRPLQGAPRLRVTLNPATGWGASDAERTSGTNHIRYLVKPQPLRLTTD
                     APVGQLIEGRSFRLEQNLHFFLGPDEPFVGHVGHSLAAMLHETADEWRGWVRGLAIPL
                     EWQRTVIRAAIALKLCQHEETGAIVAALTTSIPEAPGSGRNWDYRYCWIRDAYYTVQA
                     LNRLGALDVLEKYLGYLRNIVDEAKGGHIQPLYAVSGEAKLDEGEAEGLAGYRGMGPV
                     RVGNAAYVQIQHDAYGQIVLSNVQAFIDERLFRKATETDFRALERVGERAWEVHDQPD
                     AGLWEYRTRTAVHTYSSVMSWAACDRLANAAEVLGLDDRAALWRDRAAAIRARIEEAA
                     WYAPERRFAASFGGDELDASLLQLVDMRFLSPDDPRFLATFEAVEKGLRRGEHMLRYA
                     AEDDFGLPETAFNFCTFWLIEALHLAGREEEARELYEHMLERLTPAGLLSEDSDFATG
                     ELWGNYPQTYSLVGLINCAVLLSRPWSAVR"
     gene            complement(3022..>3732)
                     /gene="otsB"
                     /locus_tag="VGB57_05820"
     CDS             complement(3022..>3732)
                     /gene="otsB"
                     /locus_tag="VGB57_05820"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY0130905.1"
                     /translation="SRFAPAALFLDFDGTLVELADSPGAIAVPGGLAPLLDRLSRRLE
                     GRLAIVSGRAVDDLRGHLGGCAAILSGSHGAELHYADGRSVPISAPAGLAAARESVRK
                     FAAGAEGLLVEDKPTGVALHYRLAPERAEEADAFLEALAGRSGLALQRGKMVAELRPH
                     GADKGAALRRLMAEPPFAGARPVFVGDDLTDEDAFRAAAALGGEGVLVGPARPSAARW
                     RLEGVVDVTAWLEAAAYG"
BASE COUNT          597 a         1301 c         1263 g          571 t
ORIGIN      
        1 ccttcgcaac caggttcatc ccgtcgcgca gcggagtgat gagagcgatc tgcgaggcgc
       61 ggtagaaacc gcccagatcc tcgcgcgcat agccctggtt gacgtagcgg atcggcaccc
      121 agtcgacttc cgcgaagtcg ccgttgatcc gaccgctcaa ttcgtcgagc cgctcgcgga
      181 tcacttcgta ggtcgcgacc tcgccgcggg acggcggcgc gatctggagc aggaacaatt
      241 cgccccggca ttgctcgtgc tgctcaagga accggcggta accgttgaag cgttcggtca
      301 ggcccttgga ataatcgagc cggtcgaccc cgatgatcgt ccgccgcccc gccgcgctga
      361 cccgcatcct ctcgaaggcc gcctgcgccc tggggctcgc cgccgccttg gcgaattcgg
      421 aatagtcgat cccgatcgga taggcgccgg catggaccga gcgctcgccg acatggatgg
      481 tgccgtcctc gtcgcaatgt ccgccgagct cgcgctcgac ataatggcgg aagctctcca
      541 gccattcctc ggtctggaat ccgatcacgt cgtaggcgag catcgagagc accagccctt
      601 cgtggaaggg cagcgacacc atcagccgcg ccggcggcca gggaatgtgg aggaagaagc
      661 cgatccggtt cctgactcct cggtccctca attgctggcc gagcggaatg aggtggtagt
      721 catggaccca gatgagatcg tccggctcga tcaaaggctg caccgtctcg gcgaagcgct
      781 cgttcacccg ctcgtagccg ccggcgaagc tccggtcgta ttcggccagg tcgatccggt
      841 aatggaagag cggccagagc gtgcggttgg cgtagccgtt ataatattcg tcgacgtcct
      901 gctcctcgag gtcgatcgtc gcggtggtga ctccgtcgtg gcggtcgaaa tcgatatggc
      961 cggtgaattg ctcggccgtc tcgcccgacc agccgaacca cagcccgcga tggtcgcgga
     1021 gcgccgccga gagcgcgacg gcgagcccgc cctgcgaacc ctccccctcg cccttgggct
     1081 tcgacacccg gttcgagatg acgatgagcc ggctcacggt gcgctcgccc ccctctcgtc
     1141 atcccggcga aggccgggat ccaggaacac ggttccgatc gaacatgagc aatcggtgtt
     1201 catggatccc ggctttcgcc gggatgacgg gaatgcattc atctcaccgc gctccacggc
     1261 cgcgacagaa gcacggcgca attgatcagg ccgacgagcg aataggtttg cgggtagttc
     1321 ccccacagct cgccggtggc gaagtcgctg tcctcggaga gcaggccggc cggagtcagc
     1381 cgctctagca tatgctcgta aagctcgcgc gcctcctcct cccgcccggc gagatggagc
     1441 gcctcgatca gccagaaggt gcagaagttg aaggcggtct cgggcagtcc gaaatcgtcc
     1501 tcggcggcgt agcggagcat atgctcccct cgcctcagcc ccttctcgac cgcctcgaag
     1561 gtggcgagga agcggggatc gtcggggctg aggaagcgca tgtcgacgag ctggagaagg
     1621 ctcgcgtcga gctcgtcgcc gccgaagctg gcggcgaagc ggcgctccgg cgcgtaccag
     1681 gccgcttcct cgatccgggc gcggatcgcc gccgcccggt cgcgccacag ggccgcccgg
     1741 tcgtcgagcc ccagcacttc ggccgcgttg gcgagccggt cgcaggcggc ccagctcatc
     1801 accgagctgt aggtgtgtac ggcggtgcgc gtgcgatatt cccaaaggcc cgcatcgggc
     1861 tggtcgtgca cctcccaggc gcgctcgccg acccgctcga gcgcccggaa gtcggtctcc
     1921 gtcgccttcc ggaacagcct ttcgtcgatg aacgcctgga cgttcgagag cacgatctgg
     1981 ccgtaggcgt cgtgctggat ctggacgtag gcggcgtttc cgaccctcac cgggcccatg
     2041 ccgcgatagc cggccaggcc ctccgcctcg ccctcgtcga gcttcgcctc gcccgacacg
     2101 gcgtagagcg gctggatgtg gccgcccttg gcttcgtcga cgatgttcct cagatagccc
     2161 agatacttct ccaggacatc gagcgctccg agccgattca gagcctggac ggtgtaatag
     2221 gcgtcgcgga tccagcaata gcgatagtcc cagttgcgcc cgctgcccgg cgcctcggga
     2281 atcgaggtgg tgagcgcggc gacgatcgct ccggtctcct cgtgctggca aagcttgagc
     2341 gcgatcgcgg cgcggatcac ggtccgctgc cattcgagcg ggatggcgag gcccctgacc
     2401 cagccgcgcc attcgtccgc cgtctcgtgg agcatcgccg cgaggctgtg ccctacatgg
     2461 ccgacgaacg gctcgtcggg gccgaggaag aaatggaggt tctgctccag ccggaagctt
     2521 cgcccctcga tcaattggcc gaccggggcg tcggtggtga gccggagcgg ctgcggcttg
     2581 accagatagc gaatgtggtt ggtgccgctg gttcgctcgg cgtcgcttgc cccccagccg
     2641 gtggcggggt tgagggtcac ccggagccgg ggcgcgccct gcagcggcct cacgatccgg
     2701 gcgaaggcga ccgggcggta catccggccc gaccgttcga agcgcgggca gaagtcgaaa
     2761 atctcggcga ccgcgccgtc cgaatcggtc agcctcgtgg tcaggatcgc ggtgttgcgc
     2821 aaatagctct gctcggccga cacctggttg tcgaccgcga tgcgccactc gccccggggc
     2881 aggtcgcttt cgcctttggg cccgagcagc gagcagaaga gcgggtcgcc gtccacgcgc
     2941 ggagcgcagc cccagacgaa gcccgcctcg cggtcgacga gagcgctcac ctggcaattg
     3001 ccgatcggcc agagcgacag gtcagccata agccgccgcc tccagccagg cggtcacgtc
     3061 caccacgccc tccaggcgcc accgcgccgc gctcggccgg gcgggcccga ccagcacgcc
     3121 ttccccgccc agcgcagcgg ccgcccggaa tgcgtcctcg tcggtaagat cgtcgcccac
     3181 gaacacagga cgcgcacccg cgaagggagg ttccgccatc agccgccgaa gcgccgcgcc
     3241 cttgtccgcg ccgtgggggc gaagctccgc gaccatcttg ccgcgctgca aggcgaggcc
     3301 ggagcgcccg gcgagcgcct ccaggaaggc gtcggcttcc tccgcccgct ccggcgccag
     3361 ccggtaatgg agcgcgacgc ccgtgggctt gtcctcgacc agcaggccct cggcgcccgc
     3421 ggcgaatttc cggaccgact cgcgcgcggc ggccaggccg gccggcgcgg agatgggaac
     3481 cgagcgcccg tcggcataat gaagctcggc gccatgggag ccggacagaa ttgcggcgca
     3541 gccgcccaga tgccctcgga gatcgtccac cgcccggccg ctgacgatgg cgaggcgacc
     3601 ctcgagccgc ctggagagcc ggtcgagaag cggcgccagt ccgcccggga cggcgatcgc
     3661 tcccggcgag tccgcaagct cgacgagggt cccgtcgaag tcgaggaaaa gcgcggcggg
     3721 cgcgaaccgc ga
//
LOCUS       DASWNU010000296         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_321284, whole genome shotgun sequence.
ACCESSION   DASWNU010000296 DASWNU010000000
VERSION     DASWNU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321284"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGB57_05825"
     CDS             <1..824
                     /locus_tag="VGB57_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0130906.1"
                     /translation="TLAICAGFAKNRRVMVQGYHGTGKSTHIEQVAARLNWPLIRINL
                     DAHVSRIDLVGRDAIVLRDGLQVTEFKEGLLPWALQSPTAIVFDEYDAGRPDVMFVIQ
                     RVLEAEGKLTLLDQNRVIRPNPWFRLFATTNTIGLGDTTGLYHGTQQINQGQMDRWNI
                     VVTLNYLPAEIEARIVLAKSGEYDHEGGRAEVEKMIKVADLTRQGFVNGDISTVMSPR
                     TVISWAQNALIFNDVGFAFRLTFLNKCDEAERPLVAEYYQRVFGKDLPESVVAKA"
     gene            898..2730
                     /gene="cobT"
                     /locus_tag="VGB57_05830"
     CDS             898..2730
                     /gene="cobT"
                     /locus_tag="VGB57_05830"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEY0130907.1"
                     /translation="MADRDNPLEAFRQVLTGAARAIAREPELELGYSADAPSTSGKTV
                     KVPMPGRSLAPREVAEARGYADAAALKLRHHNAAMHGRGAPADEVARAVFDAVEQARV
                     EALGARAMAGVRANLAQAIEMRMRTDPITRARNRAEVPLGTALGLKVRERLTGEAPPE
                     AARGGIDLVSDWIEDKAGADLDALGLVLDDQAAFAALATKLLRDLELIEGEADADVDP
                     DSGEEGEGSDQDQGDEGDSQDEEDEGGSDGQSEMRVEQGEGDEEEGERDWSEEEMSEG
                     EEGMGEEGEEGMLPVRPNRPLSDLPPQFDYRVFTTRYDEVVEAPELCDEEELGRLRAY
                     LDQQLVHLQSAVTKLANRLQRRLMAQQNRSWDFDQEEGLLDAARLARVIVNPAHSLSY
                     KIERETEFRDTVVSLLIDNSGSMRGRPISIAAISADILARTLERCGVKVEILGFTTRA
                     WKGGQARETWLAAGRPPGPGRLNDLRHIVYKAADEPWRRARRNLGLMMREGLLKENID
                     GEALLWAHNRLIARPEERRILMVISDGAPVDDSTLSVNSGTYLERHLRQVIGWIQARS
                     PVELVAIGIGHDVTRYYDRAVTIMDAEQLGGTMIEQLAALFDGP"
     gene            complement(2899..3045)
                     /locus_tag="VGB57_05835"
     CDS             complement(2899..3045)
                     /locus_tag="VGB57_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY0130908.1"
                     /translation="MVGGVAGALLGREVTRDRYRGRSGTTGAILGGAIGALAGRAVQK
                     SSCR"
BASE COUNT          531 a         1014 c         1076 g          433 t
ORIGIN      
        1 ccaccctcgc catctgcgcg ggcttcgcca agaatcgccg ggtgatggtc cagggctatc
       61 atgggacggg caagtcgacc catatcgagc aggtcgcggc gcggctcaac tggccgctca
      121 tccggatcaa tctcgacgcc catgtcagcc gcatcgacct ggtcgggcgc gacgcgatcg
      181 tgctgcgcga tggcctccag gtgaccgagt tcaaggaggg gctgctcccc tgggcgctgc
      241 agtccccgac ggccatcgtg ttcgatgaat atgatgccgg ccgccccgac gtgatgttcg
      301 tgatccagcg cgtgctggag gcggagggca agctgaccct gctcgaccag aaccgggtga
      361 tccgccccaa tccctggttc cgcctgttcg ccaccaccaa cacgatcggc ctcggcgaca
      421 cgaccgggct ttatcatggc acccagcaga tcaatcaggg ccagatggac cgctggaaca
      481 tcgtcgttac cctgaactac ctgccggccg agatcgaagc ccgcatcgtt ctcgccaaga
      541 gcggcgagta cgaccacgaa ggcggccgcg ccgaggtcga gaagatgatc aaggtcgccg
      601 acctgacccg ccagggcttc gtcaacggcg acatctcgac cgtgatgagc ccgcgcaccg
      661 tcatcagctg ggcccagaac gccctcatct tcaacgacgt cggcttcgcc ttccgactca
      721 cgtttttgaa caagtgcgac gaggcggaac gcccgctggt cgccgaatat taccagcgcg
      781 tcttcggcaa ggacctgccg gagagcgttg tcgcgaaagc gtgatcgagc gttccccggc
      841 gaaggccggg gcccagtcaa gcggccggtc tggaccccgg ccttcgccgg ggaactgatg
      901 gccgaccgcg acaaccccct cgaagccttc cgccaggtgc tgaccggcgc cgcccgggcg
      961 atcgcccgcg agcctgagct cgagctcggc tatagcgccg atgccccctc gacgtcgggc
     1021 aagaccgtca aggtgccgat gccggggcgc tcgctggctc ccagggaggt cgcggaggct
     1081 cggggctatg ccgatgccgc ggcgctcaag ctcaggcacc acaatgcggc gatgcacggc
     1141 cgcggcgctc cggcggacga ggtcgccagg gcggtgttcg acgcggtcga gcaggcgcgg
     1201 gtagaggcgc tcggcgcgcg ggcaatggcg ggggtgcgcg ccaatctcgc gcaggcgatc
     1261 gagatgcgga tgcgcaccga cccgatcacc cgggcccgca accgggccga ggtgccgctg
     1321 ggaaccgcgc tcggcctcaa ggtccgggag cggctgaccg gcgaggctcc gcccgaggcc
     1381 gcgcgcggcg ggatcgacct cgtctccgac tggatcgagg acaaggccgg ggccgatctc
     1441 gacgcactcg ggctggtgct cgacgatcag gcggccttcg ccgcgctcgc caccaagctg
     1501 ctccgagacc tcgagctgat cgagggcgag gccgacgccg acgtcgaccc ggactccggc
     1561 gaggagggcg aagggagcga ccaggaccaa ggcgacgaag gagacagcca ggacgaggag
     1621 gacgaaggcg gttccgacgg ccagtccgag atgcgggtcg agcagggcga gggcgacgag
     1681 gaggaaggcg agcgcgactg gtccgaggag gagatgtccg aaggcgagga gggcatgggc
     1741 gaggaaggcg aggagggaat gctcccggtc cggcccaacc ggccgctctc cgacctcccg
     1801 ccgcaattcg actatcgcgt cttcacgacc agatatgacg aggtcgtcga ggctccggag
     1861 ctttgcgacg aggaggaact ggggcggctt cgagcctatc tcgaccagca gctcgtccac
     1921 ctgcagagcg cggtcaccaa gctcgccaac cggcttcagc ggcggctgat ggcgcagcag
     1981 aaccggagct gggacttcga ccaggaggag gggctgctcg acgcagcgcg gctggcgcgg
     2041 gtgatcgtca acccggccca ctcgctgagc tacaagatcg agcgcgagac cgagtttcgc
     2101 gacacggtcg tctcgctcct gatcgacaat tcgggttcga tgcggggccg gccgatctcg
     2161 atcgccgcaa tctccgccga catcctcgcc cggacgctcg agcgctgcgg ggtcaaggtc
     2221 gagatcctgg gcttcaccac ccgcgcctgg aagggcggcc aggcgcgcga gacctggctc
     2281 gcggcgggcc ggccgcccgg tccgggacgc ctcaacgacc tgaggcacat cgtctacaag
     2341 gcggcggacg agccgtggcg gcgggcgcgc aggaatctcg gcctgatgat gcgcgagggc
     2401 ctgctcaagg agaatatcga cggcgaagcg cttctatggg cgcacaaccg gctgatcgcc
     2461 cggcccgagg agcggcggat cctgatggtc atctccgacg gagcgccggt cgacgattcg
     2521 accctgtcgg tgaacagcgg cacctatctg gagcggcacc tgaggcaggt gatcggctgg
     2581 attcaggcgc gctcgccggt cgagctcgtc gcgatcggaa tcggccacga cgtcacccgc
     2641 tattatgatc gggcggtgac gatcatggac gccgagcaat tgggcgggac gatgatcgag
     2701 caattggcgg cgctcttcga cgggccttga ccgtcatccc ggcgaaggcc gggatccatg
     2761 aacacaggcc tccgcgaact taccttgatc cgtgttcatg ggtcccggct ttcgccggga
     2821 tgacgggtaa aaagaagggc cggcctccct ttcggaagac cggccccgcc ctcgatacac
     2881 taccggaggg atctggtctt agcggcagct ggacttctgg accgcgcggc ccgcgagagc
     2941 gccgatggcg ccgccgagga tcgcgccggt ggtgcccgaa cggccgcgat agcggtcgcg
     3001 ggttacttcg cggccgagca gcgctccggc gactccgccg acgatggtgc cggt
//
LOCUS       DASWNU010000297         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_115886, whole genome shotgun sequence.
ACCESSION   DASWNU010000297 DASWNU010000000
VERSION     DASWNU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115886"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VGB57_05840"
     CDS             <1..630
                     /locus_tag="VGB57_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY0130909.1"
                     /translation="DLMMTAGKEVEFLIQRLAQKARAAGIHLIMATQRPSVDVITGVI
                     KANLPTRISFQVTSKIDSRTILGEQGAEQLLGKGDMLYMPGGKQIARVHGPFVSDDEV
                     RAVAEHWRAQGQPSYVQAVTEEPEDGGYMFEGQPTGEDDPEAQLYRKAVQTVAESQKA
                     STSYLQRQLRVGYNNAARLIERMEKDGFVSAPDHVGRREVLVDSEGRPL"
     gene            761..1387
                     /locus_tag="VGB57_05845"
     CDS             761..1387
                     /locus_tag="VGB57_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0130910.1"
                     /translation="MRLKTLALAAAPVAAAAMLVPQAPVVAQAASPLNQVTAHLKAVN
                     TMVASFRQTDRKGRALTGTMTLKRPGKLRFQYQKGVPMLIVADGRKLNMIDYETGRVD
                     GWPIGNSPLGVLLNPNPDLGRIAKVVRNDSQTMLVQARDPAKPEFGTITLGFAKVRSG
                     PSGLLLQGWTVVDAQNNRTTVKLSGQRFNLPVADSAFTFQRPTKGRKG"
     gene            1613..2407
                     /locus_tag="VGB57_05850"
     CDS             1613..2407
                     /locus_tag="VGB57_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0130911.1"
                     /translation="MPSTLKIASWNINSVRARIEIVEKFLSEEAPDILCLQETKVCDS
                     DFPAAMFSRLGYVHQIVCGQRMHHGVAILSRLPAREEERHDWQANGEARHIGVRLECG
                     IRLENVYVPAGGDTPDREVNPKFGQKLDFIERMTRWSEACACPTIMVGDFNVAPLECD
                     VWNHKQLLNVVSHTPVEVEALTRLQQSNDWVDLGRHFHPAPQRLHTWWSYRSPDWTVN
                     DRGRRLDHMWATADVAAQAVRHVVHEPCRNWTRPSDHVPLVTEFRI"
     gene            2404..>3111
                     /locus_tag="VGB57_05855"
     CDS             2404..>3111
                     /locus_tag="VGB57_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY0130912.1"
                     /translation="MSGGRDVARAIDALRRGWPVRIGGTGFLAVETADESVLAAFDAG
                     GPADLLISGHRAVTLKLANQREAVPTGPVRIERMAMSLAEATAVADPALDLSRPLKGP
                     FRARDVGCDRAAGAAVALSRRAGLLPALFLDGESGEDSVSLSPEDVEAFEHAMSLRIA
                     SRARLPTRFAEQAEIVAFRSDEDGAEHVALLVGSPSGKPPLVRLHSECLTGDVLGSLK
                     CDCGPQLDAALEAIAASG"
BASE COUNT          520 a         1094 c         1027 g          470 t
ORIGIN      
        1 gacctgatga tgaccgccgg caaggaggtc gagttcctca tccagcgcct cgcgcagaag
       61 gcgcgcgccg cgggaatcca cctgatcatg gccactcagc gcccgtcggt cgacgtcatc
      121 accggagtga tcaaggccaa cctgccgacc cgcatcagct tccaggtgac cagcaagatc
      181 gacagccgca ccatccttgg cgagcagggc gccgaacagc tgctcggcaa gggcgacatg
      241 ctctacatgc ccggcggcaa gcagatcgcc cgggtccacg gccccttcgt ctccgacgac
      301 gaggtgcgcg ccgtcgccga gcattggcgg gcgcaggggc agcccagcta cgtccaggcg
      361 gtgaccgagg agccggagga cggcggctac atgttcgagg gtcagccgac cggggaggac
      421 gaccccgaag cccagctcta tcggaaggcg gtccagacgg tggcggagag ccagaaggcc
      481 tccacctcct acctgcagcg ccagctccgg gtcggctaca acaacgccgc ccggctgatc
      541 gagcggatgg agaaagacgg cttcgtcagc gcccccgacc atgtcggccg ccgcgaggtg
      601 ctggtcgatt cggaagggcg gccgctttaa ccgtcttgtc ctccctgcgc gcgaagcgcg
      661 cggggaggat caggaatggg aaccctcggg gttcagaggg cattcaagct ggaaaggcta
      721 gcgcctgacc aaacttccac ccgatggaga cttgatgatg atgcgtttga agacgctcgc
      781 cctcgccgcg gcgccggtcg cggccgccgc catgctggtg ccgcaggccc ccgtggtcgc
      841 tcaggcggcc tccccgctca accaggtgac ggcgcacctc aaagcggtga acacgatggt
      901 ggcgagcttc cgccagaccg accgcaaggg ccgggccctg acgggaacca tgaccctgaa
      961 gcggcccggc aagcttcgct tccagtatca gaagggcgtg ccgatgctga tcgtcgccga
     1021 cggccgcaag ctcaacatga tcgactatga gaccggccgg gtcgacggct ggccgatcgg
     1081 caactcgccg ctcggcgtgc ttctgaaccc gaacccggac ctcggccgga tcgccaaggt
     1141 cgtccgcaac gattcgcaga ccatgctcgt ccaggcgcgc gatcccgcga agcccgaatt
     1201 cgggacgatc acgctcggct tcgccaaggt ccggagcggc ccgtcgggtc ttctgctcca
     1261 gggctggacg gtggtcgacg cccagaacaa ccggacgacc gtcaagcttt ccggccagcg
     1321 cttcaacctg cccgtggcgg acagcgcctt cacgttccag agaccgacaa aaggtcgaaa
     1381 aggctgacgc cattcatcgg cgcgacagga atgagatagt aatacatatt ctgcgattgc
     1441 gggtggtcgc tgtcgggttt tccccctgtt gcccggcggt tccgccgcag tcgagcctcg
     1501 cgtagaccac gcttggttgg ccctcgctcc acgcccccgg agcgggggcc tttattgttg
     1561 gggacgcccc ccgtcatcga cagcaaacgc cgtcccggtt agagcctgag gcatgccttc
     1621 caccctcaag atcgcgtcat ggaacatcaa ctccgtgcgc gcccgaatcg agatcgtcga
     1681 gaaattcctg agcgaggaag cgcccgacat attgtgcctt caggagacga aggtctgcga
     1741 ctcggacttt cccgccgcca tgttcagccg cctcggctac gtccaccaga tcgtctgcgg
     1801 ccagcgcatg caccacggcg tggcgatcct gagccggctg ccggcgcggg aggaggagcg
     1861 gcacgactgg caggccaatg gcgaagcccg gcacatcgga gtccggctgg agtgcggcat
     1921 ccggctcgag aacgtctacg tgccggcggg cggcgacacg cccgatcgcg aggtcaatcc
     1981 caagttcggg cagaagctcg acttcatcga gcggatgacc cgctggtcgg aggcctgcgc
     2041 ctgccccacc atcatggtcg gcgacttcaa cgtcgcgccg ctcgaatgcg acgtgtggaa
     2101 ccacaagcag ctgctcaacg tggtcagcca cacgccggtg gaggtggagg ctctgacccg
     2161 gctccagcag agcaacgact gggtcgacct cggccgccat ttccaccccg cgccgcaacg
     2221 tctccacacc tggtggagct accgctcgcc cgactggacc gtgaacgacc gcggccgccg
     2281 cctcgaccat atgtgggcga ccgccgacgt cgctgcccag gcggttcgcc acgtcgtcca
     2341 cgagccctgc cggaactgga cccggccttc ggaccacgtg cccctggtca cggaattcag
     2401 gatttgagcg gcgggcgcga cgtcgcccgg gcgatcgacg cgctccggcg cggctggccg
     2461 gtccgaatcg gcggcaccgg cttcctcgcg gtcgagaccg ccgacgagtc ggtgctcgcc
     2521 gccttcgacg ccggggggcc ggcggatctg ctcatctcgg gccaccgcgc ggtgaccctg
     2581 aagcttgcca accagcgcga ggccgtgccg acggggccgg tcaggattga gcggatggcg
     2641 atgagcctgg ccgaggcgac ggcggtcgcc gatccggctc tcgacctctc gcgcccgctc
     2701 aaaggtcctt tccgcgccag ggatgtcggc tgcgaccgcg ccgccggcgc cgccgtcgcc
     2761 ctgtcgcgtc gtgccggcct gcttccggcc ctcttcctcg acggcgagtc gggcgaggac
     2821 tcggtctccc tctccccgga ggacgtggag gcgttcgagc acgcgatgtc gctcaggatc
     2881 gcctcgcgcg ccaggcttcc gacccgtttc gccgagcagg cggagatcgt cgccttccgc
     2941 tccgacgagg acggcgccga gcacgtcgcc ctgctggtcg gttcgccgtc gggaaagccc
     3001 ccgctggtcc gactccacag tgaatgtctg accggcgacg tgctcggatc gctcaaatgc
     3061 gactgcggac cgcagctgga cgccgctctg gaagccatcg ccgcctcggg g
//
LOCUS       DASWNU010000298         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_194309, whole genome shotgun sequence.
ACCESSION   DASWNU010000298 DASWNU010000000
VERSION     DASWNU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194309"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VGB57_05860"
     CDS             <1..924
                     /locus_tag="VGB57_05860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0130913.1"
                     /translation="LTKAVSAAFDRRTYGQGSETTAVLVVQDGKIVAERYRDDFDMHM
                     SQRTWSVAKSLAGTLVGAAVQQGLLDVNAPAPIPEWRSTGDPRSAITTDSLLRMASGL
                     HSDAAGNRTDATYFGGSSVTENATQWPLETAPNTRFRYSNNDILLAVRGLRAKLGDGE
                     RALAFPFESLLWKIGMTRTVPETDWQGNFILSSQVWTTARDLARLGLLYQNDGIWNGE
                     RILPPGWGAYVSRHGPAQPAAGFGYGATFWTFPAAAGLPADAYIAQGNRGQYLAIIPS
                     RRIVIVRRGYDGPGTGFDPAPFVRDLLAALR"
     gene            997..>1614
                     /locus_tag="VGB57_05865"
     CDS             997..>1614
                     /locus_tag="VGB57_05865"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEY0130914.1"
                     /translation="MYRTEAGDGDYWQAQLMFWGPRILAAIAILVIAWLLARAAKWAI
                     AKVVDRVPALKKHYEAEPGKTLGSLIGDIVFWLIILIGIMLAVQPLELGEVLDPVRQL
                     TTNALAFIPNVIGAGLIFFIGLIVARIVRRLVETSLVAANADGWLRKTGLSETAGTAP
                     AAAAGSPAAPGTRTSISKSVGMIVFFLIMIPVTISALDALQIEAIS"
BASE COUNT          255 a          582 c          512 g          265 t
ORIGIN      
        1 ctgacgaagg cggtgtcggc cgcgttcgac cgccgcacct acgggcaggg gagcgagacg
       61 acggcggtgc tcgtcgtcca ggacggcaag atcgtcgccg agcgctaccg ggacgatttc
      121 gacatgcaca tgtcgcagcg cacctggtcg gtcgccaaga gcctcgccgg caccctcgtc
      181 ggcgccgccg tccagcaggg ccttctggac gtgaacgccc cagcgccgat cccggaatgg
      241 cgcagcaccg gcgatccgcg ttcggcgatc accaccgaca gcctgttgcg aatggcgagc
      301 ggccttcatt cggacgcggc cggcaaccgc accgatgcca cctatttcgg cggcagctcg
      361 gtcaccgaga atgcgaccca atggccgctg gaaacggcgc cgaacacgcg cttccgctac
      421 tccaacaacg acatattgct cgccgttcgc gggcttcggg cgaagctggg cgacggcgag
      481 cgggcgctcg ccttcccgtt cgaatcgctg ctctggaaga tcggaatgac ccggacggtt
      541 ccggagacgg actggcaggg caatttcatc ctttccagcc aggtctggac caccgcccgc
      601 gaccttgccc gcctcggcct cctctaccag aatgacggga tctggaacgg cgagcgcatc
      661 ctgccgccgg gatggggcgc gtacgtctcg cgccatggcc ccgcccagcc ggcggcgggc
      721 ttcggctatg gcgccacctt ctggaccttt ccggccgccg caggccttcc cgccgacgcc
      781 tacatcgccc agggcaatcg cggacagtat ctggcgataa tcccctcgcg ccggatcgta
      841 atcgtccgcc gcggctatga cggacccggg accggcttcg atccggctcc gttcgtccgg
      901 gacctgctcg ccgccttgcg ttgagcggaa cccctgaggg cgcgcgacat tatccccgcg
      961 agcggcgcgc gccgctcgca acaaggggat tcgcctatgt accgcaccga ggccggagac
     1021 ggcgattact ggcaggccca gctcatgttc tgggggccaa ggatccttgc cgccatcgcc
     1081 attctcgtca tcgcctggct gctcgcccga gcggcgaaat gggcgatcgc caaggtcgtc
     1141 gaccgggtcc cggcgctcaa gaaacattat gaggcggaac cgggcaagac gctcggcagc
     1201 ctgatcggcg acatcgtctt ctggctcatc atcctcatcg gaatcatgct cgcggttcag
     1261 ccgctcgagc tcggcgaagt cctcgatccg gtgcgccagc tcaccaccaa cgccctggcc
     1321 ttcattccga acgtgatcgg cgcgggcctc atcttcttca tcggcctgat cgtcgcccgg
     1381 atcgtccggc ggctggtcga aacctcgctg gtggccgcca atgcggacgg ctggcttcgc
     1441 aagaccggcc tgtcggaaac cgccggcacc gcccccgcgg ccgccgcggg atccccggcg
     1501 gcgccgggca cccgcacgtc gatcagcaag tcggtcggga tgatcgtctt cttcctgatc
     1561 atgattccgg tgacgatctc ggcgctggac gcgctccaga tcgaggcgat ctcc
//
LOCUS       DASWNU010000299         6287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_219783, whole genome shotgun sequence.
ACCESSION   DASWNU010000299 DASWNU010000000
VERSION     DASWNU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219783"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..2036
                     /locus_tag="VGB57_05870"
     CDS             45..2036
                     /locus_tag="VGB57_05870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY0130915.1"
                     /translation="MIPASPAESPADPRLAFRADINGLRAIAVLAVLGFHFELAPLRG
                     GFVGVDVFLVISGYLMTGIILGRLERGSFSVAGFYLDRARRIVPALAMLLAAMLAAGA
                     FFLLPDEYALLARQAGSSALFVSNILHWRETGYFDPGLDQKWLLHSWTLSLEWQFYLL
                     YPLLLPLIVRFFPPPARKIALAAAALLSFGLMLALARLAPAAAFYLLPPRAWELLAGA
                     LVYLVPPLSGRSARIAQASGLGLIAAAVVLIDPDGWPDGRALAPVAGTMLVILAARTG
                     SRVTGNALAAWIGLNSYSIYLWHWPIVLALRRSGHAGEWPWIAAGIAAAFLLGHLSWR
                     FVERSARPRAGAPRIGTDRVAGSWRAAAAYGLPPLALALASLGIWRTHGLPQRFSAQI
                     EAVVRDSIPRPVAGAEPCYSTGTLPSVPCRLGPAGSPVLATFLGDSHADAQLLGLIEA
                     LPAGRRGAVEFNALAGCPPVLGLRPVLPRSPCGAFNARFLEPLAAPRETPLILTASWA
                     GYARQPAFRFPGEGNAPPTAASFHAALLRSTCALAAGGPTFVVLPTPQFPYWVTRKLQ
                     RRLIADPGAPDVAMPHSVHEARNRDVVAVFREAERSCGVRLLDPAPWLCRGGACQGSV
                     GHRPLYRDNHHLTDLGARLLAPMFRPVFEDRFRDSAQGR"
     gene            complement(2057..2233)
                     /locus_tag="VGB57_05875"
     CDS             complement(2057..2233)
                     /locus_tag="VGB57_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130916.1"
                     /translation="MNDDRTDRRASRAARQTREIEENQEALRRSIAETERLVSESENM
                     LRRHRKEREQDEAE"
     gene            2340..2837
                     /locus_tag="VGB57_05880"
     CDS             2340..2837
                     /locus_tag="VGB57_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130917.1"
                     /translation="MVSRFIPLILAPLLASCQPPGVAVRAAFFGNALTFVAADPGQAD
                     ATACWREGIVVDDSLRPVWRFSSPGTGECRRLFPLSYGRAPEGAESEIKASPLEPGRL
                     YLFIGDATAQVSGAFALTRAGSARIVHNVDPDSPAAAALRQGWRRRSSPGIADSPPPA
                     ADAER"
     gene            2844..3077
                     /locus_tag="VGB57_05885"
     CDS             2844..3077
                     /locus_tag="VGB57_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130918.1"
                     /translation="MRRLPLILGAVAAFATLLAVGPVAVAVALLRSSSDAQGPTLAAQ
                     ILIAILVLAVTALAGAAAWGLTHLVLRLFRRGG"
     gene            3117..3473
                     /locus_tag="VGB57_05890"
     CDS             3117..3473
                     /locus_tag="VGB57_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0130919.1"
                     /translation="MRILDVDQSPAAGAADLTTHLLTGETVHAAFVAPTGVILFTDRR
                     ILLAQREHLLEARIETSSYSYRAVRHFSIQEGEAAESRSTVRIWLGDEPQPLHLRANP
                     GTDLRPLQRLLADRLV"
     gene            complement(3612..4724)
                     /locus_tag="VGB57_05895"
     CDS             complement(3612..4724)
                     /locus_tag="VGB57_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY0130920.1"
                     /translation="MPAIRPRLRLRASRKGRSAFAAPSATRDRLADLDWTGDNPGDLN
                     ARCYVPAGIEGPAPLVVVLHGCTQDAAVYDHGSGWSTLADRHGFILLFPEQRRANNPM
                     LCFNWFSGNDTTRGRGEAASIRAMIEAMKKAHGIDPERIFVTGLSAGGAMASAMLATY
                     PEVFAAGAIIAGVAYGCAADVAEAFDCMGGRSRSDAGELGASVRRASQHKGPWPRVQV
                     WQGSADRTVVASNADAIVLQWTDLHGVGPEPDRSDRVEGYPRQAWLGSKGEPLVEQYV
                     ITGMDHGIPLDGSGEDALGEAGAHMLDVGLSSTARIAAFFGIAPETAERPQAARPAAA
                     SRPKPAAKALPQVTLPKASGPQKVIEDALRKAGLMR"
     gene            complement(4822..5091)
                     /locus_tag="VGB57_05900"
     CDS             complement(4822..5091)
                     /locus_tag="VGB57_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HEY0130921.1"
                     /translation="MRIDDANEDTTLCKVVVNHEEQYSIWPADRENPAGWNDAGRTGT
                     KAECLAYIREVWTDRRPKSLRDSMDMGLGVPVRDPEAGPNRGCPV"
     gene            5234..6124
                     /locus_tag="VGB57_05905"
     CDS             5234..6124
                     /locus_tag="VGB57_05905"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY0130922.1"
                     /translation="MTDQRSFDDRDGFIWLDGALVPWREANVHVLTHALHYASSVFEG
                     QRAYSGEVFKLSDHSRRLRNSARLLGFEIPFSVEQIDEACLEVLRANALEDAYMRPLA
                     WRGSEQMGVSAQKSKIHLAIAAWPWPAYFGEEAIAKGLRLAISKWRRPAPYTAPTGSK
                     ASGLYMICTLARHQAQADGFDDALMFDWRGQVAEATGANAFFVKDGALHTPTPDCFLD
                     GITRRTVMELARRRGIEVVERAIWPEELEGFEQFFLTGSAAEVTPVASAGPWSFEVGE
                     LTMQLRKDYLDLVHRRLPNS"
BASE COUNT          797 a         2302 c         2106 g         1082 t
ORIGIN      
        1 cgaagttggc gatgatttga gacgcatctt cgaatgacct ccccatgatt ccggcatctc
       61 ccgccgagtc gccggcggac ccgaggctcg ccttccgcgc cgacatcaac gggcttcggg
      121 cgatcgcggt gctcgcggtg ctcggctttc atttcgagct ggcgccgctg cgcggcggct
      181 tcgtcggagt cgacgtcttc ctcgtcatat cgggctatct gatgacgggg atcatcctgg
      241 gccggttgga gaggggcagt ttctccgtcg ccggcttcta cctcgaccgg gcccggcgca
      301 tcgtcccggc cctcgcaatg ctgctcgccg cgatgctggc ggccggggcg ttcttcctgc
      361 ttcccgacga atatgcgctt ctcgccaggc aggcgggaag cagcgccctg ttcgtctcca
      421 acatcctcca ctggcgcgag acaggctatt tcgatcccgg gcttgaccag aaatggctgc
      481 ttcacagctg gacgctgtcg ctcgaatggc aattctacct gctctaccct ttgcttctgc
      541 ccctgatcgt ccgcttcttc ccgccgccgg cccggaagat agcactggcc gccgccgcac
      601 tgctctcctt cggcctgatg ctcgcgctcg cccggctggc cccggccgcc gccttctatc
      661 tgctgccgcc gcgcgcctgg gagctgctgg ccggcgccct cgtctatctg gtcccgccgc
      721 tctccggccg aagcgcgcgg atcgcccagg cctcggggct cggcctgatc gccgccgcgg
      781 tcgttctgat cgatccggac ggctggcccg acggacgcgc gctcgcgccc gtcgccggga
      841 cgatgctcgt gatcctcgcc gcccgaaccg gatcgcgcgt gaccggcaat gcgctcgccg
      901 cctggatcgg gctcaattcc tattccatct atttgtggca ctggccgata gtgctcgcgc
      961 ttcggcgttc cggccatgcc ggcgaatggc cgtggatcgc cgccggaatc gccgcggcct
     1021 tcctgctcgg tcacctctcc tggcgcttcg tcgagcgctc tgccaggccg cgagccggcg
     1081 cgccgcgtat cggcacggac agggtcgcgg gctcctggcg cgccgccgcc gcctacggcc
     1141 tcccgccgct ggcgctcgct ctggcgagcc tcggcatctg gcgcacccac ggccttccgc
     1201 agcgtttctc cgcccagatc gaggcggtcg tgcgggattc gatcccgcgc ccggtggccg
     1261 gcgccgagcc ctgctactcg accgggacgc tgccgtcagt gccgtgccgg ctcgggccgg
     1321 ccggatctcc cgttctcgcg accttcctcg gcgacagcca tgccgacgct cagctgctcg
     1381 gcctcatcga ggcgctgccc gccggcaggc gcggcgctgt cgagttcaac gccctggccg
     1441 gctgtccgcc ggtgctgggc ctgcgcccag tgctgccgcg aagcccctgc ggcgccttca
     1501 acgcgcgctt cctcgagcct ctcgccgcgc ctcgcgagac cccgctcatc ctgaccgcca
     1561 gctgggccgg ctatgccagg cagcccgcct tccgctttcc aggcgaaggc aacgcgccgc
     1621 cgacggccgc ttccttccac gcggccctgc tgcgctccac ctgcgcgctc gcggcgggag
     1681 ggccgacctt tgtggtgctg ccgacgccgc aattccctta ttgggtgacc cgcaagctgc
     1741 agcgccggct gatcgccgac cccggggcgc cggacgtggc catgcctcac tcggtccatg
     1801 aggcgcgcaa tcgcgacgtc gtcgccgttt ttcgagaagc ggagcgaagc tgcggggtcc
     1861 ggctcctcga cccggcgccc tggctctgcc gtggcggcgc ctgtcagggc tcggtcggcc
     1921 accgtccgct ttatcgcgac aaccatcacc tgaccgatct tggcgcccgt ctgctggcgc
     1981 cgatgttccg tcccgtgttc gaggatcgct tccgggactc ggcccagggg cgatgagacc
     2041 ggctcggccg ccgggcttac tccgcctcgt cctgctcgcg ctccttgcgg tggcggcgga
     2101 gcatgttttc cgattcgctg accaggcgct cggtctcggc gatgctccgg cgcaacgctt
     2161 cctgattttc ctcgatctcg cgagtctgcc gggcggcgcg gctggcgcgt cgatccgtgc
     2221 gatcgtcgtt catgcctgat cctccgacgg ccgcgaggcc ggtccgcccg ggtaacttac
     2281 gcgacggcgg ccggttcccc gggctggtgc tgtgcctgga cgcgctataa gcccgcgtca
     2341 tggtcagccg tttcatcccc ttgatcctgg cgcctctgct cgcctcgtgc cagccgcccg
     2401 gggtcgcggt ccgggccgcc ttcttcggaa atgcactcac cttcgtcgcg gccgatccgg
     2461 gccaggccga tgccacggcc tgctggcgcg aagggattgt ggtcgacgac agcctgcggc
     2521 cggtgtggcg gttcagcagc cccgggacgg gcgaatgtcg acggctattc ccgctttcct
     2581 acggccgggc gcccgagggc gccgaatcgg agataaaggc gagcccgctg gagcccggcc
     2641 gcctgtatct gttcatcggc gatgccaccg cgcaagtgtc cggcgcattt gcgctcaccc
     2701 gggccgggag cgcccggatc gtccacaatg tcgacccgga ttcgccggcg gcggccgccc
     2761 ttcgccaggg ctggcggcgg cggtcgtcgc ctggaatcgc ggacagcccc ccgcccgccg
     2821 ccgacgccga gcgctagacc acggtgcgcc gccttccgct gatcctcgga gccgtggcgg
     2881 ccttcgctac gctgctggcg gtcgggcccg tggccgtcgc cgtggcgctg ctccgctcgt
     2941 cgtcggacgc acagggcccg accctcgccg cgcagattct catcgcaatc ctggtcctcg
     3001 ccgtcacggc cctggcaggg gccgcggcct ggggacttac ccatcttgtc ctccgcctct
     3061 tccgtcgcgg cggctgaggg attgccgagc cgacgccgcg ggcccattta gccgggatgc
     3121 gaatcctcga cgtcgatcaa tcgccggccg ccggcgcggc cgatctcacc acccacctcc
     3181 tgaccggaga aacggttcac gccgccttcg tcgctccgac cggagtcata ctgttcaccg
     3241 atcgccggat cctgctcgcc cagcgcgagc atctgctcga ggcccggatc gagaccagct
     3301 cctattccta ccgggcggtg cgccatttct cgatccagga aggggaggcg gccgagagcc
     3361 gctcgacggt ccgcatctgg ctcggcgacg agccccagcc gctccacctg cgcgccaatc
     3421 ccggcaccga cctgcgcccg ctccagcgcc tcctcgccga ccggctcgtc tgatcttcgt
     3481 cgccccagga gggaccccat cgaagtagta ctttgatgga acccgaagcc tgggaccccg
     3541 gcgggtgaaa aacgcaggcg cctttcaccc gctggctccc cggggttcgc cgggatgacg
     3601 ggggaagcgg ctcagcgcat caggccggcc ttgcgcagcg catcctcgat caccttttgg
     3661 gggccgctcg ccttcggcag cgtcacctgc ggcagagcct tggcggccgg cttgggacgg
     3721 gacgccgccg cgggacgggc ggcttgcggc cgctccgcgg tctcgggcgc gattccgaag
     3781 aaagcggcga tccgggccgt cgagctcagg ccgacgtcga gcatgtgcgc gccggcttcg
     3841 cccagagcgt cctcgccgct tccgtcgagc gggatcccat ggtccattcc ggtgatcaca
     3901 tattgctcga ccagcggctc gcctttcgaa cccagccagg cctggcgggg atagccttcg
     3961 acccggtcgc ttcggtccgg ctcggggccg acgccgtgga gatcggtcca ttgcaggacg
     4021 atcgcgtcgg cgttgctcgc gacgacggtc cggtcggcgc tgccctgcca gacctggacc
     4081 cgcggccacg gccccttgtg ctgggacgcc cggcgcacgc tggcgccgag ctcgccggca
     4141 tcgctcctgg agcgcccgcc catgcagtcg aaagcctcgg cgacgtcggc ggcgcagcca
     4201 taagcgactc cggcgatgat cgctccggcg gcgaagacct cgggataggt ggcgagcatc
     4261 gccgaagcca tggctccgcc ggcgctgagg ccggtgacga agatgcgctc cggatcgatg
     4321 ccgtgagcct tcttcatcgc ttcgatcatc gctcggatcg aggccgcctc gccccggccg
     4381 cgggtcgtgt cgttgccgga gaaccagttg aagcagagca tcgggttgtt ggcacggcgt
     4441 tgctccggaa agagcaggat gaagccgtgg cggtcggcga gcgtcgacca gcccgagcca
     4501 tgatcgtaga cggccgcgtc ctgggtgcag ccgtggagca cgaccaccag cggggccggc
     4561 ccctcgatcc cggccggcac gtagcagcgg gcgttgaggt cgccgggatt gtcgccggtc
     4621 cagtccagat cggcaagacg gtcgcgggtg gcggagggag cggcgaaggc gctgcgtccc
     4681 ttgcggctcg cgcggaggcg gaggcgcggt cgaatggcag gcataagctt actcccgggg
     4741 aggcccccgg gacggagggc actcggcatc ttgaacgcta tatgggcgag ctttgttgca
     4801 gcgcaacata attaccggca ctcagaccgg gcacccccgg ttcggtccgg cctcgggatc
     4861 gcgcacgggc acgccgaggc ccatatccat cgagtcgcgc aggctcttgg gccgcctgtc
     4921 ggtccagacc tccctgatgt acgccaggca ttccgccttg gtgcccgtcc tgccggcatc
     4981 gttccagccg gcgggattct cccgatcggc gggccagatc gaatattgct cctcgtggtt
     5041 gacgacgacc ttgcagaggg tcgtgtcctc gttggcgtcg tcgattcgca tccttgtcct
     5101 ccgggccgct gccgagggca tagcatattc gccgacggcc ggcgaagtcg gcccggccga
     5161 gccacccgcc cgctcaagtt gacgctcccg gcgcccggtc ctatccccgc cgcgcatctc
     5221 aagggagcct cgagtgacgg accagcgcag cttcgacgac cgcgacggct tcatctggct
     5281 ggacggagcc ctggtgccgt ggcgggaggc aaacgtgcat gtgctcaccc atgcccttca
     5341 ttatgcttcg tccgtgttcg aggggcagcg cgcttattcc ggcgaggtct tcaagctcag
     5401 cgaccatagc cggcggcttc gcaacagcgc ccggctgctc ggcttcgaga tcccgttcag
     5461 cgtcgagcag atcgacgagg cctgcctcga ggtgctcagg gccaacgctc tggaggacgc
     5521 ctatatgcgc ccgctcgctt ggcgcgggtc ggagcagatg ggggtgtcgg cccagaagtc
     5581 gaagatccac ctcgccatcg cggcctggcc gtggccggcc tatttcggcg aggaggcgat
     5641 cgccaagggc cttcgcctcg ccatctcgaa atggcggcgg cccgcgccct atacggcgcc
     5701 gaccgggtcg aaggcctcgg gcctctacat gatctgcacg ctggcccgtc accaggcgca
     5761 ggcggacggc tttgacgacg ccttgatgtt cgactggcgc ggacaggtcg ccgaggcgac
     5821 cggcgccaac gccttcttcg tcaaggacgg ggcgctccat acgccgaccc ccgactgctt
     5881 cctcgacggc atcacccgcc ggaccgtgat ggagctggcg cggcggcgag gcatcgaagt
     5941 ggtcgagcgg gcgatctggc cggaggagct cgagggcttc gagcaattct tcctcaccgg
     6001 aagcgccgcg gaggtgactc cggtcgcctc cgcgggcccg tggagcttcg aggtgggcga
     6061 gctgacgatg cagctcagga aggattatct cgacctcgtc caccggcgcc tcccgaacag
     6121 ctgatcccgc gtcgcccgag ctcgggccgg ggctccgtgg agcgaaagcg ccgcattctt
     6181 caatcgccgg ggccccagcc ttcgctgggg cggtcgacgc gggggcttcc gcccccggcg
     6241 ccgaccgtta taagccgccc cgcctgctgg ggcgtcgcca agtggta
//
LOCUS       DASWNU010000300         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_165900, whole genome shotgun sequence.
ACCESSION   DASWNU010000300 DASWNU010000000
VERSION     DASWNU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165900"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VGB57_05910"
     CDS             complement(<1..1274)
                     /locus_tag="VGB57_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0130923.1"
                     /translation="MYDYDFFSFESKADFLRRSETFWNPDKTRFWQDAGVDLVIDRRE
                     GYLFWDMAGRRLIDMHLNGGTYNLGHRNPELIAVLEQAMGRFDIGNHHFPALARTALA
                     EALVGTCTPNMRRVIYGSGGGEATDIAIKTARHTRQRRKIVSIRKGYHGHTGLAVATG
                     DDRFSTLFLSDRPDEFVQVPFNDLEAMEEALRGGDVAGVVIETIPATYGFPLPDPGYL
                     RSVKLLCERHGALYIADEVQTGLMRTGEMWGVAKAGIEPDIMVVGKGITGGMYPIACT
                     VVSEECSAWLKEDGFGHISTGGGAELGCIVALKVLEICGRPEVRSMVHYIAERFREGL
                     SAIQELYPDWLVGIRQDGVVMGLEFDHPEGAKLVMRRLYENGVWAIFSTLDPRVLQFK
                     PGILLGPDLVDEILDRTAVAIGEALAEAAPAPR"
     gene            1377..1541
                     /locus_tag="VGB57_05915"
     CDS             1377..1541
                     /locus_tag="VGB57_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130924.1"
                     /translation="MIRRLLQQFGGGTTARRPARGGRRPARGTGRRGGRGSAAGGIGG
                     VVERFIRTRR"
BASE COUNT          238 a          580 c          580 g          246 t
ORIGIN      
        1 cggggcgccg gagcggcctc ggccagcgcc tcgccgatcg cgacggcggt gcggtcgagg
       61 atctcgtcga cgagatcggg cccgagcagg atgcccggct tgaactgcag gacccgcggg
      121 tcgagcgtcg agaagatcgc ccacacgccg ttctcgtaga gccggcgcat gacgagcttg
      181 gccccctcgg ggtggtcgaa ctcgagcccc atgacgacgc cgtcctggcg gatcccgacg
      241 agccagtcgg gatacagctc ctggatggcg ctcagccctt cgcggaagcg ctcggcgatg
      301 tagtgcacca tcgatcgcac ctccgggcgg ccgcagatct cgaggacctt cagcgccacg
      361 atgcacccga gctcggcgcc gccgccggtc gagatgtggc cgaagccgtc ctccttgagc
      421 cacgcggagc actcctcgga gaccaccgtg caggcgatgg ggtacatgcc gcccgtgatc
      481 cccttgccga ccaccatgat gtccggctcg atcccggcct tggccacccc ccacatctcg
      541 ccggtccgca tgaggcccgt ctgcacctcg tcggcgatgt agagcgcccc gtggcgctcg
      601 cagagcagct tgaccgaccg caggtagccc gggtcgggca gcgggaaccc gtaggtcgcc
      661 gggatggtct cgatcaccac gcccgccacg tcgccgccgc gcagcgcctc ctccatcgcc
      721 tcgaggtcgt tgaacggcac ctggacgaac tcgtccgggc gatcggacag gaagagcgtg
      781 gagaaccgat cgtcgcccgt cgccacggcg aggccggtgt gcccgtgata gcccttgcgg
      841 atcgagacga tcttccgccg ctggcgcgtg tggcgggcgg tcttgatcgc gatgtcggtc
      901 gcctcgcccc cgcccgagcc gtagatcacg cgccgcatgt tcggggtgca cgtgccgacg
      961 agcgcctcgg cgagagcggt gcgcgcgagc gccgggaagt ggtggttgcc gatgtcgaag
     1021 cggcccatcg cctgctcgag cacggcgatg agctcgggat tgcggtgccc gaggttgtac
     1081 gtcccgccgt tgaggtgcat gtcgatgagc cgccggcccg ccatgtccca gaacaggtag
     1141 ccctcgcggc gatcgatgac gaggtccacg ccggcgtcct gccagaaccg ggtcttgtcc
     1201 gggttccaga aggtctccga gcggcgtagg aagtccgctt tggactcgaa ggagaagaag
     1261 tcgtagtcgt acacgttctg cttccggcgc ggaacgtacc ataagggcga cgactcgtcg
     1321 atctttgtcg tgattggtcc ggtttggcgc ggtggcgctc cggagaggct ccttacatga
     1381 tccgacgact tctccagcag ttcggcggcg ggaccaccgc gcgccgcccc gcccgcggcg
     1441 gacgcaggcc cgctcgcggc accggccggc gcggcggccg gggcagcgcc gccggcggta
     1501 tcggcggcgt ggtcgagcgc ttcatccgca cgcggcgctg agcctcccgg cgagcggccg
     1561 ggccccagcg gactgaccca tgcgccgccg cgcacggcgg tggcgcccat gtccggaacc
     1621 cgaccgccga agtgctgtga cggc
//
LOCUS       DASWNU010000301         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_194424, whole genome shotgun sequence.
ACCESSION   DASWNU010000301 DASWNU010000000
VERSION     DASWNU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194424"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2118)
                     /locus_tag="VGB57_05920"
     CDS             complement(<1..2118)
                     /locus_tag="VGB57_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0130925.1"
                     /translation="MASEASPPPSRLRRTLITSLKIGLYGGLIALVALVVAVGVAMSS
                     LPSYSELVRRDDLGQMIRVRAADGTELIALGPSFGQWLSYDQIPGIMKDAMVAVEDKR
                     FRSHIGVDPIGLARAARVRVSKGRWTQGGSTITQQLARNIFLTNSRTFGRKAREAVLA
                     LALERRFSKNQILELYLNRVYFGGGAYGIDAASRRFFGHSAKTLSLSEAAIIAGLVKA
                     PSNYSPTADVEAARSRAAVVIRLMQENGDISPAVAAAAEPAEVQMVATPRQNSVRYFT
                     DWVLPQLDTLIDESVEPIDVWTTLDPNMQRLGDQVINATTPAGAQGALIALDRDGSIR
                     AMIGGRDYVRSLYNRGTQATRQPGSSFKLFVYLAAIEAGYKPDDMVVDAPITINGWSP
                     RNNSRGFAGQVTVRQAFAQSINTVSVRLAQEVGFRTVADMAQRFGITSPVVTHPSMAL
                     GSSEVRLIDMTRAFASVAAKGNAVVPYGIKRVTTASGQILYQHQPDESRVLVAPWVAA
                     QMTDLLQAAVLTGTGRGAQIGRPVAGKTGTTTSNKDGWFLGFSSGLTTGVWMGRDDNK
                     AIPGLQGGRAPARAFHDFMQRAVANRPAENFEIAVQMPEWAVSEPDEETWFEAPDNGV
                     LVNPDGSGPPGGFQPLPPRQDEAEPSSDREDRLDQDFIDRAIDREPPPEPRFREEDRL
                     RDRDRFRDERERERRERRRRDPDL"
     gene            complement(2286..2663)
                     /gene="msrB"
                     /locus_tag="VGB57_05925"
     CDS             complement(2286..2663)
                     /gene="msrB"
                     /locus_tag="VGB57_05925"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0130926.1"
                     /translation="MSEREWREKLTAEQYRVLREAGTEPAFSGRYVDNHEEGEYRCAG
                     CGELLFDSEAKYDSGSGWPSFTEPAAPSVSEHADMSHGMRRVEVRCAKCDGHLGHLFP
                     DGPAPTGLRYCINSASLDFKARK"
     gene            complement(2701..4047)
                     /locus_tag="VGB57_05930"
     CDS             complement(2701..4047)
                     /locus_tag="VGB57_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0130927.1"
                     /translation="MVDSPPPARRAFGFWICLALVIGNVIGTGIFLLPAALAPYGANA
                     VAGWLLTIAGCLCLAQVFAALARALPEANGPYDYIRPALGAPAAFFVMWSYWISTWVT
                     NAAVAIGAVSYLSSLAPGFFAPFHAGPLTAIAFVILFTAIATRGAKASGEVQVATTIL
                     KLVPLIAVVALAAFVWGGHGEAAGGLAPAPLQPSSVAAAATLTLWAMLGFESATVPAG
                     RVRDPQRTVPSATLAGTFLVGIVYLMVTWAVFLLLPADEAAKSSAPLADLIGRYWGSG
                     LAHLIALFAAISALGALNGWVFLQAEVPLALARAGIFPAAFARLNRAGTPFVGHVIGC
                     ALSCALIATNLSRGMAGLFEFMILLATAATLVLYLLAAVSALVLRRRGAIGGGLVVAT
                     AIAGTLFALWTFYGAGLEATLWGLALLLSGVPVFLIMRRNLLRDRIVEATSSTAED"
     gene            complement(4115..4201)
                     /locus_tag="VGB57_05935"
     tRNA            complement(4115..4201)
                     /locus_tag="VGB57_05935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4165..4167),aa:Leu,seq:gag)
BASE COUNT          725 a         1465 c         1542 g          666 t
ORIGIN      
        1 gagatccgga tcgcgccgcc gtcgctcgcg gcgctcccgc tccctctcgt cgcggaatcg
       61 atcccgatcc cgcaatcgat cctcctcccg gaaccgcggt tccggcggcg gctcgcggtc
      121 gatcgcccgg tcgatgaagt cctggtcgag ccggtcctcc cggtcggacg agggttcggc
      181 ttcgtcctgg cggggcggaa gcggctggaa cccgcccgga ggaccgctgc cgtcgggatt
      241 gaccagcacg ccgttgtcgg gtgcttcgaa ccaggtctcc tcgtcgggct cgctgaccgc
      301 ccattcgggc atctgaaccg cgatctcgaa attctcggcc ggccggttgg ccaccgctcg
      361 ctgcatgaaa tcgtggaagg cccgggccgg cgcccgcccg ccctgcaggc cggggatcgc
      421 cttgttgtcg tcgcgcccca tccacacgcc ggtggtgagg ccgctcgaga agccgaggaa
      481 ccagccgtcc ttgttggagg tggtcgttcc ggtcttgccc gccaccggac gcccgatctg
      541 ggcgccgcgg cccgtcccgg tcaggaccgc cgcctggagc aaatcggtca tctgggccgc
      601 cacccagggc gcgaccagga cccggctctc gtccggctgg tgctggtaga ggatctgccc
      661 gctggcggtg gtgacccgct tgatcccgta gggaacgacc gcattcccct tcgcggccac
      721 cgaggcgaag gcccgggtca tgtcgatcag ccgcacctcg gagcttccga gcgccatcga
      781 gggatgggtg acgaccgggc tggtgatccc gaagcgctgc gccatgtcgg cgacggtgcg
      841 gaagccgacc tcctgggcga gccggaccga gacggtgttg atcgactggg cgaaagcctg
      901 gcggacggtc acctggccgg cgaagccgcg gctgttgttg cgggggctcc agccgttgat
      961 ggtgatcgga gcgtccacca ccatgtcatc gggcttgtag ccggcctcga tcgcggcgag
     1021 gtagacgaac aatttgaacg aggagcccgg ctggcgggtc gcctgggttc ctcgattgta
     1081 gagcgagcgg acatagtcgc ggccgccgat catcgcccgg atcgagccgt cccggtcgag
     1141 cgcgatcaga gcaccctggg cgccggccgg agtggtcgcg ttgatcacct ggtcgccgag
     1201 ccgctgcatg ttgggatcga gagtcgtcca cacatcgatc ggttcgaccg actcgtcgat
     1261 caaagtgtcg agctggggaa gcacccagtc ggtgaaatag cggacgctgt tctgcctggg
     1321 cgtcgccacc atctggacct cggccggctc ggccgcggcg gccacggccg ggctgatgtc
     1381 gccattttcc tgcatgagcc ggatgacgac cgcagcacgg ctccgcgccg cctcgacgtc
     1441 ggcggtcggc gaataattgg acggcgcctt gaccaggccg gcgatgatcg ccgcctcgga
     1501 caggctcaat gtcttcgccg aatggccgaa gaagcgccgc gacgcggcgt cgatgccgta
     1561 ggcgccgccg ccgaaataga cacggttgag atagagttcg aggatctggt tcttggagaa
     1621 gcgccgctcg agggcgagag cgagcaccgc ttcgcgcgcc ttgcggccga aggtgcggct
     1681 gttggtgagg aagatgttgc gggcgagctg ctgcgtgatg gtcgagccgc cctgggtcca
     1741 gcggcctttc gacacccgga ccctggcggc gcgggcgagg ccgatcgggt ccacgccgat
     1801 atgcgagcgg aagcgcttgt cctccaccgc gaccatcgcg tccttcatga tgccgggtat
     1861 ctggtcgtag ctcagccatt ggccgaagct tggcccgagc gcgatcagct ccgtcccgtc
     1921 ggcggcccgg acccggatca tctggccgag atcgtcgcgg cggacgagct cggagtaact
     1981 gggaagcgag ctcatcgcca ctccgaccgc cacgaccagg gcgacgaggg cgataaggcc
     2041 gccgtacaac ccgatcttga gcgaggtgat cagcgtccgg cgaaggcggg acggcggagg
     2101 agaagcttca ctggccatcg ggaagggagc ctagggccgg atgagtcggg cgacaaggga
     2161 gcggggatgg agaaggggtg gaggattttc gtcattccgg cgaaggccgg aatccatgaa
     2221 caccgaatgc ccccaattcc cctgtcggtg ttcatgggtc ccggccttcg ccgggatgac
     2281 ggcccctact tcctcgcctt gaagtccagc gaggcgctgt tgatgcagta gcgcaggccg
     2341 gtcggcgcgg gcccgtcggg gaagaggtgg ccgagatggc cgtcgcactt ggcgcagcgc
     2401 acttcgaccc ggcgcatccc gtggctcatg tcggcgtgct cgctcaccga cggggcggcc
     2461 ggttcggtga agctcggcca gccgctgccg ctgtcatatt tggcttcgct gtcgaacagc
     2521 aattcgccgc agccggcgca gcgatattcg ccctcctcgt gattgtcgac gtagcggccg
     2581 ctgaaggccg gctcggttcc ggcttcgcgg agcacccgat attgctccgc ggtcagcttc
     2641 tcgcgccact cccgctcgga cataatcgaa cgatccttta ccgggtccgc catccgaacc
     2701 tcaatcctcg gctgtcgatg aagtcgcctc aacgattcgg tcgcgcaaca ggtttcgccg
     2761 catgatcaga aagaccggga ctccgctcag aagcagcgcc aggccccaaa gcgttgcttc
     2821 gaggccggcc ccgtagaagg tccacagcgc gaacagggtt ccggcgatcg cggtcgccac
     2881 caccagcccg ccgcctatcg ccccccttcg gcgaagcacc agcgccgaga ccgcggcgag
     2941 caggtagagg acgagtgtcg ccgccgtcgc gagcaggatc atgaactcga acagccccgc
     3001 cattccccgg ctcagattgg tggcgatcaa ggcgcagctc agggcgcacc cgatgacgtg
     3061 gccgacgaac ggcgttccgg cgcggttgag cctggcgaag gcggccggaa agatcccggc
     3121 gcgcgcaagc gccagcggca cctccgcctg aaggaatacc cagccgttga gcgcgcccag
     3181 cgcgctgatc gccgcgaaca gggcgatgag gtgcgcgagg cccgagcccc agtagcgccc
     3241 gatcaggtcg gcgagcggcg cggaggactt ggccgcctcg tccgccggca gcagaaggaa
     3301 caccgcccag gtgaccatga gatagacgat cccgaccagg aaggtgcccg ccagagtggc
     3361 gctcggcacc gtccgctggg gatcgcggac gcgcccggcg gggaccgtcg ccgattcgaa
     3421 gccgagcatc gcccataagg tgagggtcgc ggcggcggcg accgaggagg gctggagcgg
     3481 cgccggcgcg agccctccgg ccgcctcgcc atgacctccc cagacgaagg ccgccagcgc
     3541 gacgaccgcg atcagcggca cgagcttcag gatcgtcgtc gccacctgga cctcgcccga
     3601 ggccttcgcg ccgcgggtgg cgatcgcggt gaagaggatg acgaaggcga tcgccgtcag
     3661 cggccccgca tggaaaggcg cgaagaagcc gggcgcgagc gaggagagat agctgacggc
     3721 gccgatcgcc accgcggcgt tcgtcaccca ggtcgagatc cagtagctcc acatcacgaa
     3781 gaaggcggcg ggagcgccga gcgcggggcg gatatagtcg tacggcccgt tcgcctccgg
     3841 caacgctcgg gcgagcgccg cgaagacctg ggcaaggcac aggcagccgg cgatggtgag
     3901 gagccagccg gcgacggcgt tcgctccgta gggggcgagg gcggcgggca gcaggaagat
     3961 gcccgtgccg atgacgttgc cgatgaccag cgcgaggcag atccagaagc cgaaggcgcg
     4021 cctggcgggc ggcggagaat caaccatcgt caccccctct tcgggggaaa cgcgccgaag
     4081 ccgtcccccg gacggcttct ttacgggcgc gatatggtgc ggtcgagaag actcgaactt
     4141 ccacggcctt tcggccacag cgacctcaac gctgcgcgtc taccagttcc gccacgaccg
     4201 cacgtcagga ctaagcgagc tggcccggtc gggacgccgc ttctggcaag gcggcgcctc
     4261 tagcaaagcg tctccatggg cgcaatggcg cgcatctgtg ccaaaccggg aaagtcctta
     4321 agactttctt taccattggg cgtccgggac gttacgacag ggccatgctg ttggttctcg
     4381 ccgtttctgc cgcactcg
//
LOCUS       DASWNU010000302         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_400213, whole genome shotgun sequence.
ACCESSION   DASWNU010000302 DASWNU010000000
VERSION     DASWNU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400213"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VGB57_05940"
     CDS             <1..1566
                     /locus_tag="VGB57_05940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY0130928.1"
                     /translation="LMHDQIRSATGFGLRAASLTSADEDRQETLQRLKRGELDLLYAA
                     PERASTGGFRELAERTPLSLIAVDEAHCVSEWGHDFRPDYRLLKPLLDAFPGVPRLAL
                     TATADRQTRADILVQLGIPDDGLIVAGFDRPNIRYHVRPRDGVGAQLKALLADQPGPG
                     IVYATSRDKTEKLAVQLAEGGRKVLPYHAGLDPRVRAANQKAFVSSEDMVMVATIAFG
                     MGIDKPDVRFVAHAGLPKSIEAFYQETGRAGRDGDPAEAWLFWGAEDFARARQRLETE
                     VPEARRPGERQRLNALAAMVETAGCRRALLLRHFGESPPERCGNCDNCLAPPVTVDAT
                     EAARKLLSAAFRTEMRFGIQHLVDVLAGRETDKVKSFGHQRVSVYGIAGEEELALMKP
                     VARALLARDAFRADPYGGLSFGPAAKPILRGEERLELVLPPARPPRKRRDRAAADFPH
                     DPLFEALRACRRDLAKEAGVPPYVIFHDSTLREMAELRPETLNALSRVSGVGQAKLER
                     YGEAFVEVVRGFA"
     gene            1680..>2072
                     /locus_tag="VGB57_05945"
     CDS             1680..>2072
                     /locus_tag="VGB57_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEY0130929.1"
                     /translation="MRKLDQTTFVSVRQLTPADVDEAAALGIRLIVNNRPDGEEPGQP
                     SSAEIEAAARAAGLDYRHIPVAAGFPPGQVEAMARALEQGPVLAFCRSGTRSTFLWAL
                     ARAARGVPAEESIAAAAVAGYDLTPIRAR"
BASE COUNT          295 a          777 c          705 g          295 t
ORIGIN      
        1 ctgatgcacg accagatccg ctcggccacc ggcttcggac tccgcgccgc ctcgctgacc
       61 tcggccgacg aggaccgcca ggaaactctg cagcgcctca aaaggggcga actcgatctt
      121 ctctatgcgg cgccggaacg ggcaagcacg ggcgggttcc gcgagctcgc cgagcggact
      181 ccgctctccc tcatcgcggt cgacgaggcg cattgcgtgt ccgaatgggg ccatgatttc
      241 aggcccgact accggctcct gaagcccctg ctcgacgcct ttccgggcgt cccgcgtctc
      301 gccctcaccg ccaccgccga ccgccagacc cgggccgaca tcctcgtcca gctcgggatc
      361 cccgacgacg gcctgatcgt cgccggcttc gaccgtccca acatccgcta ccacgtccgc
      421 ccgcgcgacg gggtaggagc gcagctcaag gcccttctag ccgaccagcc ggggccgggc
      481 atcgtctacg ccaccagccg cgacaagacc gagaagctcg ccgtccagct cgccgagggc
      541 ggacgcaagg tcctgcccta tcatgccggt ctcgacccgc gcgtgcgcgc cgccaaccag
      601 aaggcgttcg tctcgtccga ggacatggtg atggtcgcga ccatcgcctt cgggatgggg
      661 atcgacaagc cggacgtgcg cttcgtcgcc catgcgggcc tgcccaagtc gatcgaggcc
      721 ttctaccagg agacgggccg cgccggccgc gacggcgatc ccgccgaggc ctggctgttc
      781 tggggcgccg aggatttcgc ccgggcccgc cagcggctcg agaccgaggt gcccgaggcg
      841 cggcggcccg gcgagcgcca gcgcctcaac gcgctcgcgg caatggtcga gacggccggc
      901 tgccgccgag cgttgctgct ccgccacttc ggcgagagcc cgcccgagcg atgcggcaat
      961 tgcgacaatt gcctggcgcc gccggtcacc gtcgacgcca ccgaagcggc ccgaaaattg
     1021 ctctccgccg ccttccggac cgagatgcgt ttcgggatcc agcatctcgt cgacgtgctc
     1081 gccgggcggg agacggacaa ggtgaagagc ttcggccacc agcgcgtctc cgtctacggg
     1141 atcgccggcg aggaggagct ggcgctgatg aagccggtgg cccgcgccct gctcgcccgc
     1201 gacgcgttcc gcgccgatcc ttatgggggc ttgagcttcg gcccggcggc caagccgatc
     1261 ctgcgcggcg aggagaggct cgagctggtc cttcctccgg cccgtcctcc gcgcaagcgc
     1321 cgggaccgcg cagccgccga cttcccgcac gaccccctgt tcgaggcgct tcgcgcctgc
     1381 cgccgcgacc tcgccaagga ggccggggtg ccgccctacg tcatcttcca cgacagcacg
     1441 ttgagagaga tggcggagct caggccggag actttgaacg cgctgtcgcg ggtgtcgggg
     1501 gtgggccagg ccaagctcga gcgctacggc gaggcgttcg tcgaagtggt gcgggggttc
     1561 gcctgacccg tcgtcccggc gaaacccggg acctcgggga gacccgccga gacttctcgt
     1621 catgaggtcc cggctttcgc cgggatgacg gattggatcg gtcgctgtag tgagcggcga
     1681 tgcgcaagct cgatcagacc accttcgtct ccgtccgcca gctcaccccc gccgacgtgg
     1741 atgaagccgc cgctttgggc atacgcctca tcgtcaacaa ccgccccgac ggcgaggagc
     1801 ccggccagcc ctcatcggcg gagatcgagg ccgcggcccg cgccgccggc ctcgactatc
     1861 gccacatccc ggtcgccgcc ggcttcccgc ccgggcaggt tgaagcgatg gcccgggcgc
     1921 tcgagcaggg gccggtgctc gccttctgcc gctcgggcac ccgctcgacc ttcctctggg
     1981 cgctggcccg cgcggcgagg ggagtgccgg cggaggagag catcgccgcg gccgcggtgg
     2041 cgggctacga cctcacgccg atccgcgccc gg
//
LOCUS       DASWNU010000303         4602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_451572, whole genome shotgun sequence.
ACCESSION   DASWNU010000303 DASWNU010000000
VERSION     DASWNU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451572"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VGB57_05950"
     CDS             complement(<1..1308)
                     /locus_tag="VGB57_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500068.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY0130930.1"
                     /translation="MHSVTLASEDDFDGWREAARALAGSAVPAADVSWNVGGGAIDLF
                     AAPAPLPDGPAAFSVPRAFLTLAESAVCHSDPERFALLYALLLRLRERPGLIADSADP
                     LVQRLERMARAVRRDVHKMRAFLRFREVETGEGARFVAWFEPEHHIVRHNAGFFVRRF
                     ANMAWSILTPALSLHWDGETLAEGPGASRADAPEGDPIESVWKTYYASIFNPARLKRG
                     AMLKEMPKKYWKNMPETALVGELVAGAQAREAKMVEAGRGQVGGNLRAAWEAVREEAM
                     GCTRCHLYKCATRTVFGEGPLDARILFVGEQPGDQEDLAGRPFVGPAGQLFDRALGDA
                     GVDRKAAYVTNAVKHFKFEARGKRRIHSRPDAGEIEACRWWIEQERLLIRPPLTVALG
                     ATAARSIFGKAVTITAMRGRAHDLPEGGEAWVTVHPSFLFWVRD"
     gene            complement(1328..2581)
                     /locus_tag="VGB57_05955"
     CDS             complement(1328..2581)
                     /locus_tag="VGB57_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEY0130931.1"
                     /translation="MSQLDLQQKLAILADAAKYDASCASSGTSKRDSRGGPNGRGGIG
                     STEGMGICHAYAPDGRCISLLKILLTNSCIFDCHYCINRKSSNVRRARFTAEEVVRLT
                     LAFYRRNYIEGLFLSSGIIRSPDYTMEQIVEVARSLREDHDFRGYIHLKTIPDADPEL
                     VRLAGVHADRVSINVELPTAAGLARLAPEKDGDRIEAAMKDMRVAIDDGADAKKRFKS
                     APGFAPAGQSTQMIVGADAASDSDIVARASTLYDRFRLRRVYYSAFSPIPDASAVLPL
                     RRPPLMREHRLYQSDWLMRFYGFSPAEVASAADPAGMLPLDIDPKLAWALKFRESFPV
                     DVNRAPREALLRVPGLGVKAVDKLLKSRRWRRLRLEDLARLTVSIAKVRPFICAEGWQ
                     PTLFTDRANLGAWLKPKTDQLELFA"
     gene            complement(2960..4471)
                     /locus_tag="VGB57_05960"
     CDS             complement(2960..4471)
                     /locus_tag="VGB57_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY0130932.1"
                     /translation="MTVADATPDFEAVADRNDRAFLGHPKGLGYLAFIEACERFSYYS
                     MQSLLTLYMFKYLLVPGRMEGVIGLEWLRSWHYEGLSGQPLSSAIFGDYTSLVYLTPI
                     FGGIIADRFAGRRAALFAGGVTMSIGHFLMAFEGAFLFALLALIVGVGLFKGNIASQV
                     GALYKDNDLRRAMAFQIFYIAINVSVIVAPLISGTLGEKVGWHYGFGTAGIVMVLGVL
                     IFLAGRPHLPVEEGESESIGPAKTASTMAVGGLVTALVIAFVFLAGIWIALAALVPLY
                     FWLTHRLAREEKMRVLALILLIPAMAVALLTNQQIFNAYLIWADQHFQLTFFGRTLPT
                     TYMVTIDATLSFSMLVAVAAFFKWYGTKRREPDELGKMIIGSFFTIAGGLCLYIAALT
                     QGDGKIGLFWPLMFHLFNSIGFSCILPVSLALFSKIAPRQINATVIGLYYLAFFGANK
                     IVGQIGQLYSTLDTPTFWLLHVATAVVGLVAFALFKLFMSKRMAEHHEAAAAA"
BASE COUNT          841 a         1393 c         1647 g          721 t
ORIGIN      
        1 atcccgcacc cagaacagga agctcgggtg gaccgtcacc caggcctcgc cgccctcggg
       61 cagatcgtgg gcccggccgc gcatggcggt gatcgtcacc gccttgccga agatcgagcg
      121 ggcggcggtg gcgcccagag cgacggtgag gggcgggcgg atgaggaggc gctcctgctc
      181 gatccaccag cggcacgcct cgatctcgcc cgcgtcgggc ctggaatgga tccggcgctt
      241 gccgcgagcc tcgaacttga aatgcttgac ggcgttggtg acgtaggcgg ccttgcggtc
      301 gacaccggca tcgccgagcg cccggtcgaa gagctggccg gcggggccga cgaacggccg
      361 gccggcgagg tcctcctggt cgcccggctg ctcgccgacg aacaggatcc tggcgtcgag
      421 ggggccttcg ccgaataccg tccgggtggc gcatttgtag aggtggcagc gggtgcagcc
      481 catcgcctcc tcgcgaaccg cctcccaggc ggctctgagg ttgcctccga cctgcccgcg
      541 tcctgcctcg accatcttcg cctcgcgtgc ctgcgccccc gcgaccagct cccccaccaa
      601 cgcggtctcg ggcatgtttt tccagtattt cttcggcatc tccttgagca tggcgccgcg
      661 cttgaggcgg gcggggttga agatggaggc gtaataggtc ttccagacgg actcgatcgg
      721 gtcgccctcc ggagcgtcgg cccgggaggc gccggggcct tcggcgagcg tttcgccgtc
      781 ccagtggagc gacaatgcgg gggtcaggat agaccaggcc atgttggcga agcggcggac
      841 gaagaagccg gcattgtggc ggacgatgtg atgctcgggc tcgaaccagg cgacgaagcg
      901 ggcgccttcg ccggtttcga cctcgcggaa gcgcaggaag gcccgcatct tgtggacgtc
      961 gcgccgaacc gccctggcca ttcgctccag ccgctggacg agcggatcgg cggagtcggc
     1021 gatcaggccc ggccgttcgc ggaggcggag gagcagggcg tagagcaagg cgaaacgctc
     1081 gggatcggaa tggcagaccg cggattccgc aagggtgagg aaggcgcggg ggacggagaa
     1141 ggccgccggc ccgtcaggaa ggggagcagg ggcagcgaag agatcgatgg cgcccccgcc
     1201 gacattccag ctgacgtcgg ccgccggcac ggctgagccc gcgagggccc gggcggcctc
     1261 acgccagccg tcgaaatcgt cttcgctggc gagagtgacg ctgtgcatcg gtcccgaaag
     1321 cattcgctca ggcgaagagt tccaactgat ccgtcttcgg cttgagccag gcccccagat
     1381 ttgcccggtc ggtgaaaagg gtcggctgcc agccctcggc gcagatgaac ggccgcacct
     1441 tcgcgatcga gacggtgagc cgggccaggt cctccagccg gaggcggcgc cagcggcggc
     1501 tcttcagaag cttgtccacc gccttgacgc cgagaccggg gacccgaagc agcgcctcgc
     1561 gcggcgcccg gttgacgtcg accgggaagc tttcgcggaa cttgagggcc caggcgagct
     1621 tggggtcgat atcgagcggc agcatgccgg ccggatcggc ggccgaagcg acctcggccg
     1681 gcgaaaagcc gtagaaccgc atcagccagt ccgactggta gagccggtgc tcgcgcatca
     1741 gcggcggccg gcgaagcggg aggacggcgg acgcgtccgg aatgggcgag aaagcggaat
     1801 aatagacccg gcgaagccgg aagcggtcgt agagggtgct ggcgcgggcg acgatatcgg
     1861 agtcggaagc ggcatcggcg ccgacgatca tctgggtcga ctggccggcg ggagcgaaac
     1921 cgggggccga tttgaagcgc ttcttcgcgt cggcgccgtc gtcgatcgcg acacgcatgt
     1981 ccttcatcgc cgcttcgata cgatcgccgt ccttttccgg cgcgagtcgc gcgaggccgg
     2041 cggcggtggg aagctcgacg ttgatcgaga cccggtcggc atggacaccg gcgaggcgga
     2101 ccagctcggg atcggcgtcg gggatggtct tcaggtggat atagccgcgg aaatcgtgat
     2161 cctcgcgcag gcttcgggcc acctcgacga tctgctccat cgtgtagtcg ggcgagcgga
     2221 tgatcccgga ggagaggaac aggccttcga tataattgcg gcggtagaag gcgagggtga
     2281 gccgaacgac ctcctcggcg gtaaagcggg cccggcggac gttcgagctc ttgcggttga
     2341 tgcaataatg gcagtcgaag atgcagctgt tggtcagaag gatcttcagc agcgagatgc
     2401 agcgcccgtc gggagcgtag gcgtggcaga tgcccatacc ctcggtggag ccgatcccgc
     2461 ccctgccatt tgggccgccc cggctgtcgc gcttcgaggt gccggacgag gcgcaggacg
     2521 cgtcatattt ggccgcgtcg gcgaggatgg cgagcttctg ctggaggtcg agctgggaca
     2581 cgggttcgac atatgttctc gaaccgggtc ttgcaaggac cctgcccgga atgggccgag
     2641 gcgcttaaca tgttcctaat atgttcttga ataatcgatc cgagtttgga atagccgatt
     2701 caccgattcg cgatttcgcc ccagcggatc ggctttatgg tggaggggct ctcctgcgcg
     2761 ggggagcctc tccaccaacc ttttggcgag ggcgatcagc cgccgcctcc gccgcagcct
     2821 ccgccgcaac cgcagcctcc gtccgcgtcg ccgtgactgg cgcggggcct gtcatcgatt
     2881 ccgccctatt gcgcggagat gacggtttca tgacagcttt tgacctcccc gcgagatcgc
     2941 ggggaggaat ctggacggtt caagccgccg cggcggcctc gtgatgctcg gccattcgct
     3001 tcgacatgaa cagcttgaac agagcgaagg cgacgaggcc taccacggcg gtggcgacgt
     3061 ggagcagcca gaaggtcggc gtgtccaggg tcgaatagag ctggccgatc tgcccgacga
     3121 tcttgttggc cccgaagaag gcgaggtaat agaggccgat gacggtggcg ttgatctgcc
     3181 ggggcgcgat cttcgagaaa agcgcgaggc tcaccggcag gatgcacgag aagccgatgc
     3241 tgttgaacag gtggaacatg agcggccaga acaggccgat cttgccgtcg ccctgggtga
     3301 gcgcggcgat gtagaggcac aggcctccgg cgatggtgaa gaagctgccg atgatcatct
     3361 tgccgagctc gtcgggctcg cggcgcttcg tgccgtacca tttgaagaag gcggcgacgg
     3421 cgacgagcat agagaagctc agggtcgcgt cgatggtgac catataggtg gtgggaagcg
     3481 tacggccgaa gaacgttagc tggaagtgct gatcggccca gatcagatag gcgttgaaga
     3541 tctgctggtt ggtcaggagt gcgacggcca tggccgggat cagcaggatc agggccagga
     3601 ctcgcatttt ctcttcgcgc gccagacggt gggtcagcca gaaatagagg gggacaaggg
     3661 ccgccagagc gatccagatc ccggccagga acacgaatgc gattaccagc gccgtgacga
     3721 ggccacccac ggccatcgtg ctcgcagtct tggcggggcc gatcgattcg ctctcgccct
     3781 cctcgaccgg cagatgcggc cggccggcga ggaagatcag gaccccgagc accatgacga
     3841 tccccgccgt gccgaagccg taatgccagc ccactttttc gccgagcgtg ccgctgatca
     3901 gcggagcgac gatgacgctg acgttgatgg cgatgtagaa gatctggaag gccatggccc
     3961 gtcgaagatc attatccttg tagagggcgc cgacctggct cgcgatattg cccttgaaca
     4021 ggccgactcc gacgatcagg gcgagcaggg cgaagaggaa ggcgccttcg aaggccatca
     4081 ggaaatggcc gatcgacatc gtgaccccgc cggcgaaaag ggcggcgcgg cggccggcga
     4141 agcggtcggc gatgattcca ccgaagatcg gagtcagata gaccagcgag gtatagtcgc
     4201 cgaagatcgc cgatgagagc ggctgtccgc tcagcccctc gtaatgccag gatcgaagcc
     4261 actcgagccc gatcaccccc tccatccgcc ccggcaccag caggtacttg aacatgtaga
     4321 gcgtcagcag gctctgcatc gaataataag agaagcgctc gcaggcctcg atgaaggcga
     4381 gatagccgag ccccttggga tgaccgagga aggcgcggtc gttgcggtcg gctacggcct
     4441 cgaaatctgg ggtggcgtcg gcgacggtca tgcgcgctcc ttcggcaaaa tcgcgcggaa
     4501 gctagcggat cgttacggcg gcgccagcga aaacgtcagc cgaacagcca gcggccgagc
     4561 atgcgctcga aggggaaggt gaaggcgccg gcgatgatca gg
//
LOCUS       DASWNU010000304         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_374404, whole genome shotgun sequence.
ACCESSION   DASWNU010000304 DASWNU010000000
VERSION     DASWNU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374404"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VGB57_05965"
     CDS             <1..290
                     /locus_tag="VGB57_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130933.1"
                     /translation="HLSGHIYLVTNAEGQYRIAILGRPSRAGTLNGVLTTLASGSGAQ
                     LIPATTPIALLPMSRLTEAEVGLIRPGDKCHAEYSEHVAAVRDRGYAMFPY"
     gene            complement(434..2248)
                     /locus_tag="VGB57_05970"
     CDS             complement(434..2248)
                     /locus_tag="VGB57_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0130934.1"
                     /translation="MAPILDRPDARDPQSYARPLEARVTHVALDLKADFEAKRIAGTA
                     TLDVEARPDAKSIVLDSKGLEVRSVTDEGGKPLRFAVGKADPNLGAPLTVELDGARRI
                     RIAYASAADAEALQWLTPEQTAGKKHPFLFSQGQAILNRSWIPTQDSPGIRQTWEARI
                     VVPEPLRAVMSGERLTPEGEPAEGGGRAFRFRMDKPVAPYLIALAAGDIAFQSLGPRT
                     GVYAEPATIRAAAAELVDSEKMVAAAETLYGPYRWGRYDMIVLPPAFPYGGMENPTLT
                     FLTPTFLAGDRSLVGLVAHELAHSWSGNLVTNAVWPDSWLNEGFTSYFENRIMEALYG
                     PGRASQEAALSFADMESAFTELGATAAGTRLHNPDETTEGGASGIVYDKGALFLRTME
                     RIAGRERFDAWLRSYFDRHAFQPMTSALFLADLRANLVKGDKALEQKLMLDDWVYKPG
                     LPANAVRPDPKAFAAVDAVLDNVKGGAPAPSGLWPNWTTAERLRFLNGLPRQLPPERL
                     AELDRALGLTTSRNAETLFAWLQLALANRYQPAVPATERFLMAMGRRKFVAPLFETLS
                     KQGEWGRPIARRIYARARPTYHSVTTGTVDKTLKEGGA"
BASE COUNT          341 a          770 c          815 g          375 t
ORIGIN      
        1 cccacctgtc ggggcacatc tacctggtga ccaacgccga ggggcagtat cggatcgcga
       61 tcctcggccg gccgtcgcgg gcggggaccc tgaacggggt gctcacgact ctcgccagcg
      121 gcagcggcgc gcagctgatc ccggcgacca ccccgatcgc cctgctcccg atgagccgct
      181 tgaccgaggc ggaggtcgga ctgatccggc ccggcgacaa atgccatgcc gaatattcgg
      241 agcatgtcgc cgccgtccgg gatcgcggct acgcgatgtt cccttattga gggcttcaaa
      301 tcctccccgg catttgccgg ggagggggac catgcgaagc atggtggagg ggccttactg
      361 attccgaaga acccctccac cgcgcttcgc gcggtccccc tcccccggaa atccggggga
      421 ggaatttagg tgcctaggcc ccgccctcct tcagcgtctt gtcgaccgtc cccgtcgtca
      481 ccgaatgata ggtcggccga gccctggcgt agatgcgccg ggcgatcggg cggccccatt
      541 cgccctgctt cgaaagcgtt tcgaacaggg gcgcgacgaa cttcctgcgg cccatcgcca
      601 tgaggaagcg ctcggtggcg ggaacggcgg gctggtagcg gttggcgagg gcgagctgga
      661 gccaggcgaa gagcgtctcg gcgttgcggc tcgtcgtcag ccccagcgcc cggtcgagct
      721 cggcgagccg ctccggcggc agctggcgcg gcaggccgtt gaggaagcgc agccgctccg
      781 ccgtcgtcca gttcggccag agacccgaag gcgccggagc gccgcccttc acgttgtcga
      841 ggaccgcgtc gaccgcggcg aaagccttgg gatcggggcg gacggcattg gccgggaggc
      901 cgggcttgta gacccagtcg tcgagcatca gcttctgctc cagcgccttg tcgcccttga
      961 ccagattggc gcgcagatcg gcgaggaaga gcgcggaggt catcggctgg aaggcgtggc
     1021 gatcgaaata ggagcgaagc cacgcatcga accgctcccg cccggcgatc cgctccatcg
     1081 tccgaaggaa cagcgcgccc ttgtcgtaga cgatcccgct ggcgccgcct tcggtggtct
     1141 cgtccggatt gtggagccgg gtgccggccg ccgtggcgcc gagctcggtg aaggcgctct
     1201 ccatgtctgc gaaggacagg gccgcctcct ggctggcgcg tccggggccg tagagcgcct
     1261 ccatgatcct gttctcgaaa taagaggtga agccctcgtt gagccaggaa tcgggccaga
     1321 cggcgttggt gacgagattc cccgaccagc tgtgcgccag ctcgtgagcg acgagaccga
     1381 ccaggcttcg gtcgccggcg aggaaggtcg gggtgaggaa ggtcagggtc ggattctcca
     1441 tcccgccata agggaaagcg ggcggaagga cgatcatgtc gtagcggccc cagcgatagg
     1501 gtccgtagag cgtctcggcg gcggcgacca tcttctcgct gtcgacgagc tcggcggcgg
     1561 cggccctgat cgttgccggt tcggcataga ctccggtgcg ggggccgagg ctctggaagg
     1621 cgatgtcgcc ggccgcgagc gcgatcagat agggcgccac cggcttgtcc atccggaatc
     1681 ggaaggcgcg gccgccgccc tcggccggct cgccctcggg cgtcagccgc tcgccgctca
     1741 tcaccgccct gagcggctcc ggaacgacga tccgcgcctc ccatgtctga cggatcccgg
     1801 ggctgtcctg ggtcgggatc cagctccggt tgaggatcgc ctggccctgg ctgaacagga
     1861 agggatgctt cttgccggcg gtctgctcgg gcgtaagcca ttgcagcgcc tcggcgtcgg
     1921 cagcgcttgc ataggcgatt cggatgcgcc tggcgccgtc gagctccacc gtcaggggag
     1981 cgccgaggtt cggatcggct ttccccacag cgaaacgaag cggcttgccg ccctcgtcgg
     2041 tgacggacct cacctcgagc cccttgctgt cgagcacgat cgacttcgca tccggcctgg
     2101 cctcgacgtc gagggtcgcg gtgccggcga tccgcttcgc ctcgaaatcg gccttgagat
     2161 cgagcgcgac gtgggtcacc cgcgcctcga gcggacgggc gtagctttgc gggtccctgg
     2221 catcgggcct gtcgaggatc ggagccacct gccgctctcc cgaagcggct tcctgccggt
     2281 tcgatgcgcc ttggtccgcg g
//
LOCUS       DASWNU010000305         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_348350, whole genome shotgun sequence.
ACCESSION   DASWNU010000305 DASWNU010000000
VERSION     DASWNU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348350"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1387)
                     /locus_tag="VGB57_05975"
     CDS             complement(287..1387)
                     /locus_tag="VGB57_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0130935.1"
                     /translation="MASLELSNVSKAYGETLVLDDVSLTMETKEFVAFLGPSGSGKST
                     LLRIIAGLETADGGEVRLEGERIDQLPPGRRGVAMVFQHYALYPHMTVRENMAFGLRN
                     AKVPKDEIERRVADAARVLEIEEQLDKKPGQMSGGQRQRVAIGRAIVKNPKLFLLDEP
                     LSNLDAALRMRTRVELAQLRQRVDAALIMVTHDQAEAMTLADRIVVMNDRRIQQVGPP
                     MEIYSRPANAFVARFVGSPAMNLAPATLVEGGGDFARVKLGDGSEVETRVARAGLPAD
                     GPLQIGLRPESVRVAGGGASGTAARVDLVERLGERTLIYARLSDGQAITAEDEGLSRI
                     RIGDTIGLRIEGGSAHLFGPDGTGYHAEASPE"
     gene            1703..2896
                     /locus_tag="VGB57_05980"
     CDS             1703..2896
                     /locus_tag="VGB57_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130936.1"
                     /translation="MSKRLFACFALLASVSVPTLAQEGPDPVVESMMKSDGVSSEEAR
                     RRLGLLEEANRIGNRMEAEEPVRYAGIRVRQGRDFAIFVRIVGEGESILKRYTSDPAF
                     RAEKADTPLRALRNKQQSLINAMDKKTEFAVSLDVAAGKVRVLVLDAAAVKGRLQSSG
                     LLASDVIVEEVPDVISPAVAIVGGYQVRGKTYAVSGGTVSSNASLGFNIVHVPSNVRG
                     VLTAGHFDECKFAVAVTGCVPNSPATDAKSGATLDFQAQRLGGEYDYEWRTSGGNTFS
                     NQIWYGANMAVTQVYDVEAYQPNTLSVCKYGYATGYTCGLVENPREFVSDPAYGISGY
                     FARVKRNTTGNMADSGDSGGPVFGNATAFGLVTAKFTSGTFAGQMAFMPIKRIGPDLR
                     VLTTP"
     gene            2908..3336
                     /locus_tag="VGB57_05985"
     CDS             2908..3336
                     /locus_tag="VGB57_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY0130937.1"
                     /translation="MISKLTRLLPLCFAAVLTACFGGAAAVPPRPLASVLPDTSWKLA
                     AHDGAAVPSLVTIRFERDGSVSGLAICNSFSGQARFSEQAIQLSNLTQTLLGCELTGH
                     CSAQETVKALLALERVPARVTPAGLELNVNGRRLLLVPQR"
     gene            complement(3712..>4070)
                     /locus_tag="VGB57_05990"
     CDS             complement(3712..>4070)
                     /locus_tag="VGB57_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEY0130938.1"
                     /translation="ASSVAKRVVERMDAFLSETDEKSRMPSFGKVALLAVVGNEDGAH
                     NVSAQLYQALADTGWTIPPSGTCYWVGEAMGDVDFQDLPRVPDKVAQIAVMAASGAAH
                     LAALLKERPYPGVEEG"
BASE COUNT          689 a         1408 c         1298 g          675 t
ORIGIN      
        1 gccgaacagg tcgaccctct gcacgctgag cgccatgccc ctcagcagcg ggtagatcag
       61 gatgatcaca taggcggtga ggaaaggcag gacgaagagc atcccgctcc agtccctgcg
      121 ctccgccgcc gccttcattc cgcctccccg tcattccggc gaaggccgga atccatgaac
      181 gccgacggcc gaaaccggat acggcggtgt tcatggatcc cgggtcgagc ccgggatgac
      241 gaggctcgtc tgcccgttcc ctttcgcagg agcatgaacg ccaacgtcat tccggactcg
      301 cctcggcgtg atagccggtc ccgtccgggc cgaagaggtg ggccgagccg ccctcgatcc
      361 tcaggccgat cgtgtcgccg atcctgatcc ggctgagccc ctcgtcctcc gccgtgatcg
      421 cctggccgtc ggaaagccgg gcgtagatca gggtccgctc gccgagccgc tcgaccagat
      481 cgaccctggc ggcggtaccg ctggcgccgc cgcccgcgac ccggacgctc tcgggcctga
      541 ggccgatctg cagcggcccg tccgccggca gccccgcccg agccaccctt gtctccactt
      601 ccgacccgtc gccgagcttc acccgggcga aatcgccgcc gccctccacc agggtcgccg
      661 gggccaggtt catcgccggg gagccgacga agcgggcgac gaaggcgttg gccggacggc
      721 tgtaaatctc catcggcggc cccacctgct ggatccgccg gtcgttcatc accacgatcc
      781 ggtcggccag agtcatcgcc tcggcctggt cgtgggtgac catgatcagg gccgcgtcga
      841 cccgctggcg aagctgggcg agctcgaccc gggtgcgcat ccgaagcgcc gcgtcgaggt
      901 tggacaaggg ctcgtcgagc aggaacagct tcgggttctt gacgatcgcc cggccgatcg
      961 cgacccgctg gcgctggccg cccgacatct gccccggctt cttgtcgagc tgctcctcga
     1021 tctcgagcac ccgggcggcg tcggcaaccc gccgctcgat ctcgtccttc ggcaccttcg
     1081 cgttcctcag cccgaaggcc atgttctcgc gcaccgtcat atgcgggtag agcgcataat
     1141 gctggaacac catcgccacg ccgcgccggc cgggcggaag ctggtcgatc cgctcaccct
     1201 ccagccgaac ctcgcccccg tcggcggtct cgagcccggc gatgatcctg agcagggtcg
     1261 acttgcccga cccggagggc ccgaggaagg cgacgaattc cttcgtctcc atggtcagcg
     1321 agacgtcgtc gagaacgagc gtctcgccat aggccttgct gacgttgcta agctccagac
     1381 tggccaatga tttccccttc gcggcggcga tttgtcggac aagtcgggag cgccgtcaat
     1441 gggcgcgccc ttcatgctga acttgtttca gcatccagtg tcgagccgcc tcgcgggcgg
     1501 aagcccggat cttgaaacac gttcagggtg acgcgcggcg ccggcacccg acccggctcg
     1561 accgccgccc gcttcccacc ccggcaacaa gaggaaaaaa atgagaacaa atagggaact
     1621 cctaatggtc tgtcgggtta tgaatggctc tgtcgctcgg catcctgccc tggcgatccc
     1681 attgaaagca aagaggttca tgatgagtaa gcgtttgttt gcgtgcttcg cccttctcgc
     1741 gtcggtttca gtccccaccc tggcgcagga gggacccgat ccggtggtcg aaagcatgat
     1801 gaagtcggac ggtgtgtctt cggaggaggc gcgcaggcgc ctcgggctgc tcgaggaagc
     1861 caacaggatc ggcaaccgga tggaggccga ggagcccgtc cgttacgccg gcatcagggt
     1921 gcggcaagga cgcgatttcg cgattttcgt gaggatcgtc ggcgaaggcg agtccatcct
     1981 gaagcgctat acgtcggatc ccgccttccg ggcggagaag gccgacacgc ccctcagggc
     2041 gcttcgcaac aagcagcagt cgctgatcaa cgccatggac aagaagaccg aattcgccgt
     2101 cagtctcgac gtcgccgccg gaaaggtccg ggtgctcgtc ctcgacgccg cggcggtcaa
     2161 ggggcggctt cagagctccg gcctgctcgc cagcgacgtc atcgtcgaag aggtgccgga
     2221 tgtgatcagc ccggcggtgg cgatcgtcgg cggctaccag gtgcgcggga aaacctatgc
     2281 ggtcagcggc ggcacggtca gttccaatgc cagcctcggc ttcaacatcg tccatgtgcc
     2341 gagcaacgtc cgcggtgtgc tgaccgcggg ccatttcgac gagtgcaagt tcgccgtcgc
     2401 cgtgaccggc tgcgtcccga attccccggc cacggatgcc aagagcggag ccaccctcga
     2461 tttccaggcg cagcgactgg gcggcgagta tgattacgag tggcggacga gcggcggcaa
     2521 caccttctcc aaccagatct ggtacggcgc caacatggcc gtcacccagg tctacgacgt
     2581 cgaggcctat cagccgaaca ccctgagcgt ctgcaaatat ggctacgcca ccggctatac
     2641 gtgcgggctc gttgaaaatc cgcgcgaatt cgtcagcgat cccgcttacg ggatctccgg
     2701 ctatttcgcc cgggtcaagc gcaacacgac gggcaacatg gccgactccg gcgacagcgg
     2761 cgggccggtc ttcggcaacg cgaccgcctt cggcctggtc acggcgaaat tcaccagcgg
     2821 cacctttgcg ggacagatgg cgttcatgcc gatcaagcgt atcggacccg acctgcgcgt
     2881 tctgaccacc ccgtaaggcg agaagctatg atttcgaaac tgacccgatt gctcccgctg
     2941 tgcttcgcgg cggttctgac ggcctgtttc gggggggctg cggcggtgcc gccgcggccc
     3001 ctcgcctccg tcttgccgga cacgagctgg aagcttgccg ctcatgatgg cgcggccgtc
     3061 ccttcgctgg tcacgattcg cttcgaacgg gatggatcgg tgtcggggct ggcgatctgc
     3121 aacagctttt ccggccaggc gcgcttctcg gagcaggcca tccagctctc gaacctgacc
     3181 cagaccctgc tcggctgcga gctgaccggc cattgctccg cgcaggagac ggtaaaggcg
     3241 ctgctcgctc tggaaagggt gccggccagg gtgacgccgg ccgggctgga gctgaacgtg
     3301 aacgggcgcc ggctgctgct ggtgccgcag cgctgacgac gtcccgcccc acgccggccg
     3361 ctcgcgggcg gacacctgaa ccccacccgt catcctgaac ttgtttcagg atccagcgtc
     3421 gagccactcg gccgcccgac gccattccat cgagcgcgtc gcctctccgg ccggcaagcc
     3481 ctgaatttct tgagagaagg cgcgaaggca caaaggcgcg acgtagaaaa aaggagccct
     3541 ctacgttgtg ccttcgtgcc ttctctttga aattggcgcc gaggcgccgg agagcgggcg
     3601 caagcgttgg tcccacagcc cgacctccga agcggagaaa catccagcgc catgcgccca
     3661 ttcagatcgt tggggagagc gaaaaagccg tgttccgccg gccgggcaac gtcagccctc
     3721 ctccactccg gggtaaggcc gctccttgag cagcgccgcg agatgggccg cgccgctcgc
     3781 cgccatcacg gcgatctggg cgaccttgtc cggcacccgc ggcaaatcct ggaaatcgac
     3841 gtcgcccatc gcctcgccga cccaatagca ggtccccgag ggcggaatcg tccagccggt
     3901 gtccgccagc gcctggtaga gctgggcgga aacgttgtgg gcgccgtcct cattccccac
     3961 caccgccagc agcgccacct tgccgaagct cggcatccgc gacttctcgt cggtctcgga
     4021 caggaaggcg tccatccgct cgacgacccg cttagcgacg ctcgacgcct
//
LOCUS       DASWNU010000306         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_194619, whole genome shotgun sequence.
ACCESSION   DASWNU010000306 DASWNU010000000
VERSION     DASWNU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194619"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /gene="parC"
                     /locus_tag="VGB57_05995"
     CDS             complement(<1..1806)
                     /gene="parC"
                     /locus_tag="VGB57_05995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY0130939.1"
                     /translation="MSDTVNDIDPIQNHRFEDALSQRYLVYALSTITARSLPDVRDGL
                     KPVHRRLLWAMRLLRLDPAAGYKKCARVVGDTIGKYHPHGDQSVYDAMVRLAQDFSLR
                     YPLVDGQGNFGNIDGDNAAAYRYTEARLTPAAIELMAGLDEGTVDYRPTYNNEEEEPE
                     IFPGLFPNLLANGASGIAVGMATSIPPHNLAEIVDAAIVLIRDPKADHAALMEKVQGP
                     DFPTGGVLVDSPEVIASAYATGRGAFRVRGRFSTGKDADSNWESTGIEKLPQGMWQLI
                     ISEIPYQVPKNKLIEQIAALISDKKLPILADVRDESDARIRIVIEPRSRTIDPQMLKE
                     SLFRLTDLEVRVPLNMNVLDADRTPRVMGLKEVLSAWIAHQFSVLMRRSQYRIGKIDD
                     RLELLEGYIIAFLNLDRVIEIIRTQDEPKPVMIAEFALNDRQAEAILNMRLRSLRRLE
                     EMQLRGERDKLIAEREGLEALVNDPKLQRRRLVKDLTALRDRYGPATGLGRRRTLIEE
                     AAPAREIPLEAMIEREPITIIMSQRGWIRAMKGHVDPAEAEALKFKEGDGGRFFFHAH
                     TTDKLLLAAADGRFYTLAADRLPGGRGFGEPIKLMV"
     gene            1921..2832
                     /locus_tag="VGB57_06000"
     CDS             1921..2832
                     /locus_tag="VGB57_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY0130940.1"
                     /translation="MRKALCLGLLLATAACGQGQNDAGYSGRGSDGNMTTMDAASESA
                     VDSSSRASSGPPGIAPTAAPGVAFNYRYAFRLPGENISRVQEEHAQACEKMGVELCRI
                     TAMTYTDQGDGDVEAQLAFKLDPARARTFGRDGIAAVTKAEGELLHAAITGTDVGSEI
                     ASANRGQAQQSDEVGRIEQQLARPGLSSGERVELQQQLQALRDSMRAGQAEQTQRRQL
                     LASTPVVFEYQAGKTGSRLERAVADAADNFAGAAITAAIVLVTLLPWLVLLLLLWLLW
                     RWLNRRFGITGRYPEPREPAEASPPEA"
     gene            complement(2813..3736)
                     /gene="gcvA"
                     /locus_tag="VGB57_06005"
     CDS             complement(2813..3736)
                     /gene="gcvA"
                     /locus_tag="VGB57_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEY0130941.1"
                     /translation="MRHLPPLPSVRVFEAAARHENFTLAAGELGMTQAAVSYQIRLLE
                     ERLGVPLFVRSKRRVTLSEAGRRLAPVVSDAFDRLSEGFSGLVDEDESVLAISTAQTF
                     ASNWLAPRLGGFQIARPELAVRLLTSNAMTDFARDEVDVAVRMGKGPWPGLRQHFLFC
                     LYATPLCTPDFRDRHRLERPEDLLRVPRLNAHDWWWKQWLEEAGVALEDEAPRQGIRL
                     DSQALEGNAALAGHGVGLLTPLYWRGELDAGRLVQPFDLVVVSGPALWLVYPEQKRGR
                     AKIRAFRDWLLAEMTAEAERAPADAFRPPEG"
     gene            3862..4026
                     /locus_tag="VGB57_06010"
     CDS             3862..4026
                     /locus_tag="VGB57_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130942.1"
                     /translation="MRDEIDSRIWAEHGHAFSEDLANFFANIGAGFRRLNEIQFDAPW
                     KRDARGPGHA"
     gene            4053..4187
                     /locus_tag="VGB57_06015"
     CDS             4053..4187
                     /locus_tag="VGB57_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130943.1"
                     /translation="MSSLAPRLAAILLALGLTLSGVSLGASGSAPTLPDPIAPFGCYA
                     "
BASE COUNT          694 a         1389 c         1436 g          686 t
ORIGIN      
        1 gaccatcagc ttgatcggct cgccgaagcc gcgcccaccc ggcaggcggt cggcggcgag
       61 cgtgtagaat cggccgtcgg cggccgcgag cagcagtttg tcggtggtgt gggcgtggaa
      121 gaagaagcgg ccgccgtcgc cctccttgaa cttcagggcc tccgcctcgg ccgggtcgac
      181 atggcccttc atcgctcgaa tccagccgcg ctgcgacatg atgatcgtga tcggctcgcg
      241 ctcgatcatc gcctcgagcg ggatttcccg ggccggcgcg gcctcctcga tcagggtgcg
      301 gcggcggccg agcccggtcg ccgggccgta gcggtcgcga agcgcggtca ggtccttgac
      361 caggcgcctg cgctgaagct tcgggtcgtt gaccaaggct tcgagcccct cccgctcggc
      421 gatgagcttg tcccgctccc cgcgcagctg catctcctcg agcctgcgca acgagcgcag
      481 gcgcatgttg aggatggctt ccgcctggcg gtcgttgagc gcgaactccg cgatcatcac
      541 cggcttgggc tcgtcttgcg tgcggatgat ctcgatcacc cggtcgagat tgaggaaggc
      601 aatgatatag ccttcgagaa gctcgagccg gtcgtcgatc ttcccgatcc ggtattgcga
      661 ccgcctcatc agcacgctga actgatgcgc gatccatgcc gataggacct ccttcaggcc
      721 catcacccgg ggagtccggt cggcgtcgag cacgttcatg ttgagcggca cgcgcacctc
      781 gagatcggtc agccggaaca ggctttcctt gagcatctgc gggtcgatcg tccggctgcg
      841 cggctcgatg acgatccgta tccgggcgtc cgactcgtcg cggacgtcgg cgaggatggg
      901 caatttcttg tcggagatca gggccgcgat ctgctcgatc agcttgttct tgggcacctg
      961 gtaagggatt tcggagatga tgagctgcca catgccctgc ggcagcttct cgatgccggt
     1021 cgattcccag ttcgaatcgg catccttccc ggtcgagaag cggccgcgga cccgaaaggc
     1081 gccgcgtccg gtcgcataag cggaggcgat gacctcgggg ctgtcgacca gcaccccgcc
     1141 ggtcgggaaa tcgggtccct gcaccttctc catcagggcg gcatgatcgg ccttggggtc
     1201 cctgatcagg acgatcgccg cgtcgacgat ctcggcgagg ttgtggggcg ggatcgaggt
     1261 cgccattccg accgcgattc cgctggcgcc attggcgagg aggttcggga acaggcccgg
     1321 aaagatctcg ggctcctcct cctcattatt gtaggtggga cgatagtcga ccgtgccttc
     1381 gtcgagcccg gccatcaatt cgatcgccgc cggggtcagc ctggcctcgg tgtagcggta
     1441 ggccgcggcg ttatcgccgt cgatattgcc gaaattgccc tgaccgtcga cgagcgggta
     1501 gcggagcgag aaatcctggg cgagcctaac catcgcatcg tagaccgact ggtcgccatg
     1561 cggatgatat ttgccgatcg tgtcgccgac tacccgggcg catttcttgt agcccgcagc
     1621 cggatcgagg cggagcaggc gcatcgccca gagcaggcgg cggtggaccg gcttcagccc
     1681 gtcgcggacg tcgggcagcg agcgggccgt gatcgtggag agggcgtaga cgaggtagcg
     1741 ctgcgagagc gcgtcttcga aacggtggtt ctggatcgga tcgatatcgt tgacggtgtc
     1801 ggacatgccg gaccgaatag cagcgggatc gaaatgggac acccctcttg ccattgtcta
     1861 ccaataagat aatgttacaa atagccggca gagaccggtc ttaggcaaga ggagagaagg
     1921 atgcgcaagg cactctgttt gggcctcctg ctggccaccg ccgcctgcgg ccagggccag
     1981 aacgacgcag gctattccgg ccgcggcagc gacggcaaca tgaccacgat ggacgccgcc
     2041 tcggagtcgg cggtggattc cagttcgaga gcgtcttccg gtccccccgg gatcgcgccc
     2101 accgccgccc cgggagtcgc cttcaactat cgctatgcct tccgcctccc cggcgagaat
     2161 atcagccggg tccaggaaga gcatgcccag gcctgcgaga agatgggggt cgagctgtgc
     2221 cggatcacgg cgatgaccta taccgaccag ggtgacggcg acgtcgaggc ccagctcgcc
     2281 ttcaagctcg atcccgcccg ggcccgcacc ttcggccgcg acgggatcgc cgccgtcacc
     2341 aaggccgaag gcgaattgct ccacgcggcg atcaccggaa ccgacgtcgg cagcgagatc
     2401 gcctcggcga atcgcggtca ggcgcagcag tcggacgagg tcgggcggat cgagcagcag
     2461 ctcgcccgtc ccggcctgag ctccggcgag cgggtcgagc tgcagcagca gcttcaggcg
     2521 cttcgcgact cgatgcgcgc cggccaggcg gagcagactc agcggcgcca gcttctggcg
     2581 agcacgccgg tggtgttcga atatcaggcg ggcaagaccg gctcccggct cgagcgagcg
     2641 gtggccgacg cggccgacaa tttcgccgga gccgcaatca cggccgccat cgtgctggtg
     2701 acgttgctgc cgtggctcgt ccttctgctt ctgctctggc tgctgtggcg ctggctcaac
     2761 cggcgcttcg ggatcaccgg ccgctatccg gagccgcgcg agccggccga ggctagccct
     2821 ccggaggcct gaacgcgtcc gccggggccc tctcggcctc ggcggtcatt tccgccagca
     2881 gccagtcgcg aaacgcccgg atcttggccc ggccgcgctt ctgctcggga tagacgagcc
     2941 acaaagcggg accgctgacg acgacgaggt cgaagggctg gacgagccgc ccggcgtcga
     3001 gttcgccccg ccagtaaagc ggggtgagca ggccgacacc atggccggcg agcgcggcat
     3061 tgccctcgag cgcctgcgaa tcgaggcgga tgccctgccg cggcgcctcg tcctcaaggg
     3121 cgactccggc ctcctcgagc cactgcttcc accaccagtc gtgcgcgttg agccggggca
     3181 cccgaagcag atcctcgggg cgctcgagcc ggtggcggtc gcggaaatcg ggggtgcaga
     3241 gcggagtcgc gtagaggcag aagaggaaat gctgcctgag gcccggccac gggccttttc
     3301 ccatccgcac cgcgacgtcg acctcgtcgc gggcgaagtc ggtcatggcg ttggaggtga
     3361 gaagccggac cgccagctcg ggccgggcga tctggaagcc gccgagccgg ggcgcgagcc
     3421 agttggaggc gaaagtctgg gcggtgctga tggcgaggac gctctcatcc tcgtcgacca
     3481 ggccggaaaa gccttcgctg agccggtcga acgcgtcgga gacgaccggg gccagccgcc
     3541 gccccgcctc gctaagggtc acccgccgct tggaacggac gaacaaaggg accccgagcc
     3601 gctcctcgag cagtcggatc tggtagctga ccgctgcctg ggtcattccg agctcgcccg
     3661 ccgcgagcgt gaaattctcg tgccgggcag cggcctcgaa gacccggacg gagggcaagg
     3721 gcgggaggtg ccgcatcagc aggtgataaa ctcagcttat cacctgcgtc cagcctttag
     3781 ttggacggtg cgccagccct gctccatctc gctcctacga cgcgccgcac cgggcgcgcc
     3841 atcgattgaa ggagtgataa aatgcgcgac gagatcgaca gccgcatctg ggccgagcac
     3901 ggccatgctt tttccgagga tctggcgaac ttcttcgcca atatcggagc gggtttcagg
     3961 cgcctcaacg agatccagtt cgacgcgccg tggaagcgcg acgcccgcgg acccggacac
     4021 gcctgagttc cgagcgatca aggagaaatg aaatgagcag cctcgccccc cggctggcag
     4081 cgatcctcct cgccctcggc ctgaccctct cgggcgtgag cctcggcgcc agcgggtccg
     4141 ccccgaccct gcccgatccg atcgccccgt tcggctgcta cgcctgaacc gcagccgcag
     4201 gcgag
//
LOCUS       DASWNU010000307         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_504717, whole genome shotgun sequence.
ACCESSION   DASWNU010000307 DASWNU010000000
VERSION     DASWNU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504717"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VGB57_06020"
     CDS             <1..641
                     /locus_tag="VGB57_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEY0130944.1"
                     /translation="VDLGRDFDLRPVDVLDPASLGKGRLLFLAQPQRLAPAELAALDG
                     WIRGGGRALILTDPMLAWPSELPPGDIRRPPPVGLLAPLLGHWRVALEPPADSGEVAA
                     RWKGRRIRLDSPGRLRSASPDCIAEEAWTALCRLGRGTVRLVADADLMRDPLWTSSAA
                     DNPAVVGEWLDALAGLSRPRAGRTERTWAAPAMALLVLIAGIGLLLRRRRKR"
     gene            891..1376
                     /locus_tag="VGB57_06025"
     CDS             891..1376
                     /locus_tag="VGB57_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY0130945.1"
                     /translation="MEVEDFFQGSALNVVDAKGRLSVPAFVRSVIERRSDSRAIVVGA
                     HEIAPCLTAYGRNYAKNLWTDLERRRLAEEDRGGTLDDHYARARRMFGMTEDVPYDPS
                     GRIVLPPMMRRKGKIEDLALFVGVGGIVEIWNPRVALVEGDRDLKEIAAWRLEEKGIA
                     P"
     gene            1373..>1593
                     /gene="mraW"
                     /locus_tag="VGB57_06030"
     CDS             1373..>1593
                     /gene="mraW"
                     /locus_tag="VGB57_06030"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY0130946.1"
                     /translation="MSPALDNPAVPAPHVPVLLGEVIAALAVASGETHVDGTFGAGGY
                     TRAILEKGAARVYAFDRDPDAITEGEALAA"
BASE COUNT          248 a          545 c          552 g          248 t
ORIGIN      
        1 atgtggacct tggcagggat ttcgacctgc ggccggtcga tgtgctcgac ccggcgagcc
       61 tcgggaaggg gcggctgctg ttcctggccc agccgcagcg gctcgcgccc gctgagctgg
      121 cggcgctcga cggctggatc cgcggcggcg gccgcgcgct tatcctcacc gatccgatgc
      181 tggcctggcc gagcgagctt ccgcccgggg acatccggcg gccgccgccg gtcggcctgc
      241 tcgcgcccct gctcggccat tggcgggtgg cgcttgagcc tccggcggac tcgggcgagg
      301 tcgcggcgcg gtggaagggc aggcggatcc ggctcgactc gcccggccgg ctgcgcagcg
      361 ccagcccgga ctgcatcgcc gaggaggcgt ggacggccct gtgcaggctg gggcggggga
      421 cggtgcggct cgtcgccgac gccgacctga tgcgcgaccc gctgtggacg tcgagcgccg
      481 cggacaatcc ggcggtggtc ggcgagtggc tggacgcgct ggccggactg tcgaggcccc
      541 gagcgggccg gacggagcgg acctgggccg cgcccgccat ggctctgctc gtcctcatcg
      601 ccggaatcgg actcttgctg cggcggcggc ggaagcgcta accacatact tatccacagg
      661 cgaaacagcc gagaacaaga tttgaacatc gctaggaaca aaccacgaaa taccctctga
      721 aggcatgaaa tccccaaaaa aggcatgaaa tccccttccc ttgggggtcc gccgatgtta
      781 tccacgtctt ctgatttttg gggcatcttc tcggctgacc ggcgagtcac gcggcgacct
      841 ttgagtttcc gcgaacgggg gagctccgtg tatgggagcg gacaggggcg gtggaggtcg
      901 aggacttctt ccagggaagc gcgctcaacg tagtggacgc caaagggcgt ctctccgttc
      961 ccgccttcgt ccgctccgtc atcgagcgcc gttccgactc ccgggcgatc gtcgtcggtg
     1021 cccatgagat cgcgccctgc ctgaccgcct acgggcgcaa ttacgcgaag aatctgtgga
     1081 ccgacctcga gcgccgccgc ctcgccgagg aagatcgcgg cggaaccctc gacgaccatt
     1141 atgcccgggc ccggcgcatg ttcggaatga ccgaggacgt gccctacgat ccctcgggcc
     1201 ggatcgtcct tccgccgatg atgcgccgca aggggaagat cgaagacctc gcactgttcg
     1261 tcggagtggg cgggatcgtc gagatatgga acccgcgtgt cgccctcgtc gaaggcgacc
     1321 gcgacctcaa ggagatcgcc gcctggcgcc tcgaggaaaa gggcatcgcg ccatgagccc
     1381 cgccctggac aatcccgccg tgcccgcccc gcacgtcccg gtgctcctcg gcgaggtgat
     1441 cgccgccctc gccgtcgcct ccggcgaaac ccatgtcgac ggcaccttcg gcgccggcgg
     1501 ctatacgcgc gccattctcg aaaaaggagc tgccagggtc tacgccttcg accgcgatcc
     1561 cgatgcgata acagaaggcg aggcgctggc ggc
//
LOCUS       DASWNU010000308         5188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_348569, whole genome shotgun sequence.
ACCESSION   DASWNU010000308 DASWNU010000000
VERSION     DASWNU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348569"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VGB57_06035"
     CDS             <1..608
                     /locus_tag="VGB57_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0130947.1"
                     /translation="VEDGSLKVLRILSRASAVIAEGEVDQLTAQRRVETTEDHYLSII
                     SAKTAALFAAACRIAAVVAERDEGVEEGLDAYGRNLGIAFQLVDDAIDYSSDAATMGK
                     GVGDDFRDGKVTLPVILAHARGSESERRFWQEAMEGSRTSDEDLAHATHLLEESDALA
                     DTIDRARTYGQRAIDALGALPNGKAKAALTEAVEFAIARAY"
     gene            695..3211
                     /gene="hrpB"
                     /locus_tag="VGB57_06040"
     CDS             695..3211
                     /gene="hrpB"
                     /locus_tag="VGB57_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEY0130948.1"
                     /translation="MTTLLPIHAVLPDLLAALRRTSNAVLVAPPGAGKTTAVAPELLT
                     EPWCAGEILLLSPRRLAARAAAERMAELAGERVGETIGYATRLDTKRSARTRILVLTE
                     GIFRNRIQADPELAGVSAVLFDEVHERSLDGDFGLALALDAQSALRPDLRLVAMSATL
                     DGERFAALMGGAPTVESEGRSFGIELRHLGRSAEKRIEDEMAAAIRRALGEAEGSLLA
                     FLPGVAEIERTAERLEGLPADVDLHRLHGSLEPGAQRSAIAAPPPGRRKIVLATSIAE
                     TSLTLDGVRIVVDSGLARRPHYDRGAGITRLVTERASRAAVTQRAGRAGRQGPGTVYR
                     LWEEAATAGLPRFDPPEILEADLSALLLDCALWGVADPRELRWLDPPPAAAVDEARRR
                     LLSLGALGEDGRPTGHGKAIAALPLPPRLAHMLVEARGLGWGRTAAEVAVLLSERGLG
                     GNDVDLESRLRRWRAERGQRAEAARGLAKRWEQLARHSGESRDLGSTSHNGSPRSPGF
                     RRDDGSQIGPCLALAFPDRLSKRRDSSGENWISAGGRGFRLDPQSPLARETWLAVGEV
                     SGTAAGARILSAGAIAEAEVEALFGDGIESGARLSFDPATGTVQARRGRRLGAITLSE
                     GQDSKASPDEVAAALVEGVRAHGLDLLPWSDSARSLRRRSAFARGHDESLPDLSDEAL
                     LAALDEWLPMLVAGRRSLRDVDPAALSAMLDGRMGWEGRKSVDRLAPHAFESPAGSSH
                     PIDYEAEAGPTVTARVQALYGLSAHPAIAGGRVPLVLSLTSPAGRPIQTTRDLPGFWS
                     GSWSAVAKEMRGRYPRHPWPDDPAAADPTLRTKKAQFRRP"
     gene            3360..3641
                     /locus_tag="VGB57_06045"
     CDS             3360..3641
                     /locus_tag="VGB57_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEY0130949.1"
                     /translation="MAAARIFQRSRNAMQSGNARTGEWVLQFESHRPHVPDPLTGWSG
                     GGDTQAQVTIAFPTLEAAQAYADKNGISVHLVPPAERKLKLQAYADNFK"
     gene            3622..4005
                     /locus_tag="VGB57_06050"
     CDS             3622..4005
                     /locus_tag="VGB57_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130950.1"
                     /translation="MPTISNEPLGLRAALALLLLGASGAALASGTIEIDPRIEAFASR
                     AACEKALERRHKAALARVAALPAEERRTSRVDALKRDGDGLGYSEVLGLTGDASEIGL
                     PGSQTESFICRGSTLEHRIDYKAGG"
     gene            complement(4095..4613)
                     /locus_tag="VGB57_06055"
     CDS             complement(4095..4613)
                     /locus_tag="VGB57_06055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0130951.1"
                     /translation="MSEAPEVVWSGRFIEAKKLGTWEFVSRTRGVSAAVILAVDEGHV
                     LLVEQYRVPIGARCLELPAGLVGDEEEGEAAASAAIRELEEETGYTAQRAVDLGRFHA
                     SPGMSSEGFTLLRAEGLARVGEGGGVAGEDIEVHRVKLEEVAAFVAARREAGVAMDSK
                     LLLLLGAGMLTA"
     gene            complement(4610..>5188)
                     /locus_tag="VGB57_06060"
     CDS             complement(4610..>5188)
                     /locus_tag="VGB57_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130952.1"
                     /translation="PRSDAYHDVGLHYAVLAMLLVPTIAAVLPQGWIDWALGLVFGWN
                     ATLSFRLLMLLLVVKMAIVFLIVRFAFAWMPLRMALTPASTKTRRVRRRAVELFRAGT
                     EKRTKGRTGILLYLSLLEHRAEIVADEAIHGRVEPDVWGEAMAALVEGVKSGKAGEGM
                     ALAVGKIGEVLAQCLPPTLDNPNELPDRLIEL"
BASE COUNT          796 a         1947 c         1734 g          711 t
ORIGIN      
        1 tggtggagga tggcagcctc aaagtgctgc gcatcctgtc gcgcgcgtcg gccgtgatcg
       61 cggagggcga ggtcgaccag ctcaccgccc agcgccgggt ggagaccacc gaagatcatt
      121 acctgtcgat catcagcgcc aagaccgctg ccctgttcgc cgccgcatgc cgcatcgccg
      181 ccgtggtcgc ggaacgcgac gaaggcgtcg aggaagggct cgacgcttat ggccgcaatc
      241 tcggcatcgc cttccaactg gtcgacgacg ccatcgacta ttcctcggac gccgccacca
      301 tgggcaaggg cgtcggcgac gacttccgcg acggcaaggt gaccctgccc gtcatcctcg
      361 cccacgccag gggttccgag tcggaacgcc gcttctggca ggaggcgatg gaaggcagcc
      421 gcacctccga cgaggatctc gcccacgcca cccatttgct cgaggagagc gacgccctcg
      481 ccgacacgat cgaccgcgcc cgcacctatg gccagcgcgc catcgacgcg cttggcgccc
      541 ttcctaacgg caaggccaag gcggcgctca ccgaagcggt cgagttcgcg atcgcccggg
      601 cttattgatc tcccgtcgcc ccagcgaagg ctggggcacg gggggtgaag aacgctagtg
      661 cttttcaccc gccgggatgc cagccttcgc tggcatgacg acattgctcc ccatccacgc
      721 cgtcctcccc gacctcctcg ccgccttgcg ccgcacgtcc aacgccgtgc tcgtcgctcc
      781 gcccggcgcc ggcaagacca ccgccgtcgc ccccgaattg ctcaccgagc cgtggtgcgc
      841 cggcgagatc ctgctcctct ccccccgccg cctcgccgcc cgcgccgccg ccgagcggat
      901 ggccgagctc gccggcgagc gggtcggtga gacgatcggc tatgcgaccc ggctcgacac
      961 caaacgctcg gcgcgcaccc gcatcctcgt cctcaccgaa ggcattttca ggaaccggat
     1021 ccaggccgat ccggagctgg ccggggtctc agccgtcttg ttcgacgagg tccacgagcg
     1081 cagcctcgac ggcgatttcg gccttgccct cgccctcgac gcccaaagcg cgcttcggcc
     1141 cgatctgagg ctcgtcgcaa tgtcggcgac cctggacgga gagcgtttcg ccgctctcat
     1201 gggcggcgcg ccgacggtcg aaagcgaagg ccggagcttc ggaatcgagc ttcgccatct
     1261 cggccgctcc gccgagaaga ggatcgagga cgagatggcg gccgcgatcc gccgcgccct
     1321 gggcgaagcc gagggaagcc tgctcgcctt cctgccgggg gttgccgaga tcgagcgaac
     1381 cgccgagcgg ctcgaagggc ttccggccga tgtcgacctc caccgcctcc acggaagcct
     1441 cgagccgggc gcccagcgct ccgccatcgc cgcgcctccg ccgggccggc gcaagatcgt
     1501 gctcgccacc tcgatcgccg agaccagcct gaccctggac ggggtccgga tcgtcgtcga
     1561 ttccggcctc gcccgccgcc cccactacga ccggggggcg gggatcacgc gcctcgtcac
     1621 cgagcgcgcc agccgcgccg ccgtcaccca gagggccgga cgcgccggac gccaggggcc
     1681 ggggacggtc taccggctct gggaggaagc ggcgaccgcg ggcctgccgc gcttcgatcc
     1741 gccagaaatt ctggaagcgg acctcagcgc gctcctgctc gactgcgccc tgtggggcgt
     1801 ggccgatccg cgcgagctgc gctggctcga tccgcccccc gccgccgccg tcgacgaagc
     1861 gcgccgccgc ctcctctccc tcggcgccct cggcgaggac ggccgcccga ccggccacgg
     1921 caaggcgatc gccgctctgc ccttgccgcc gcgcctcgcc cacatgctgg tcgaggcccg
     1981 gggactcggc tggggccgga cggcggccga ggtggcggtc ttgctgtcgg aacgcgggct
     2041 cggcggcaac gacgtcgatc tggagtcgag gctgaggcgc tggcgcgccg agcgggggca
     2101 acgggcggag gcggcgagag gattggcgaa gcgatgggag cagctcgccc gccattccgg
     2161 cgaaagccgg gacctagggt caacaagtca caatgggtct ccacgaagtc cgggctttcg
     2221 ccgggatgac ggcagccaga tcggcccctg cctcgccctc gctttccccg accgactgtc
     2281 gaagcgccgc gactcctccg gggagaattg gatcagcgcc ggcggccgcg gcttccggct
     2341 cgatccgcaa tcgccgctcg cccgcgaaac ctggctcgcg gtgggcgagg tgagcggaac
     2401 cgccgccgga gcccggatcc tctccgccgg cgcgatcgcc gaggccgagg tcgaagccct
     2461 gttcggggat gggatcgaga gcggcgcccg attgagcttc gatcccgcca ccggtaccgt
     2521 ccaggcccgc cgcggacgcc ggctcggcgc gatcaccctt tccgaagggc aggacagcaa
     2581 ggcctcgccg gacgaagtcg ccgcggccct cgtcgaaggg gtgcgagccc acggcctcga
     2641 cctcctcccc tggagcgact ccgcgcgctc gcttcgccgc cgctccgcct tcgcccgcgg
     2701 ccacgacgag tcgctgcccg acctctccga cgaggccctg ctcgcggccc tcgacgaatg
     2761 gctgcccatg ctcgtcgccg gccggcgaag ccttcgcgac gtcgatcccg ccgccttgtc
     2821 ggccatgctc gacggccgca tgggctggga aggccgcaag agcgtcgacc gactcgcccc
     2881 tcatgccttc gagtcgcccg caggcagcag ccaccccatc gactatgagg ccgaggccgg
     2941 cccgaccgtg accgcccggg tgcaggcgct ttacggcctg tcggcgcacc ccgccatcgc
     3001 cggcgggcgg gtgccgctcg tcctcagcct gacctccccg gccggccgac cgatccagac
     3061 cactcgcgac cttcccggct tctggagcgg gagctggtcg gcggtggcga aggagatgcg
     3121 cggccgctac cccaggcacc cgtggccgga cgatccggcc gcggccgatc cgacgctgag
     3181 gacgaagaag gcgcagttcc gtcgcccctg accgtcgtcc cggcgaaagc cgggacctca
     3241 ggacgagaag tctcggcggg cctccccgag gtcccggctt tcgccgggat gacggagaca
     3301 gaaggttgat taaactggaa aagccgggca ccaaaggccg acgagaagga gcttcggaaa
     3361 tggcggcggc gcggatcttc cagcggtcca ggaatgcgat gcagtcgggc aatgcgcgca
     3421 ccggcgagtg ggtgctccag ttcgaatcgc accgtccgca cgtgcccgat ccgctcaccg
     3481 gctggtcggg cggcggcgac acccaggcgc aggtgacgat cgccttcccg accctcgaag
     3541 ccgcccaggc ctatgccgac aagaacggaa tctccgtcca cctcgtcccg cccgcggagc
     3601 gcaagctcaa gctccaggcc tatgccgaca atttcaaatg agccgcttgg cctgagggcg
     3661 gccctcgccc tgcttttgct cggtgcgagc ggcgccgcgt tggcgagcgg aaccatcgag
     3721 atcgatccga ggatcgaggc tttcgccagc cgcgccgcct gcgaaaaggc gctggagcgc
     3781 cggcataagg cggcgctggc gcgggtggcg gccttgcccg ccgaggaacg gaggaccagc
     3841 cgggtggacg ctctgaagcg ggacggcgac ggcctcggct attcggaagt gctcggcctc
     3901 accggcgatg cgtcggagat agggctgccg ggcagccaga cggaatcctt catctgccgc
     3961 ggaagcacgc tggaacaccg aatcgactac aaagccggcg gctagcatcc gtcgtcccgg
     4021 cgaaagccgg gacctcgggg agacccactg ggcctttttg cgtcctgggg tcccggcttc
     4081 cgccgggatg acggtcaggc ggtgagcatc cccgctccca gcagaagcag aagcttcgaa
     4141 tccatcgcca cgccagcctc gcggcgagcg gcgacgaagg ccgccacctc ctccagcttc
     4201 acccggtgca cctcgatatc ctcgccggcc actccgccgc cctcgccgac gcgggccagc
     4261 ccctcggcgc ggagcagggt gaagccttcc gacgacattc ccggggaagc gtggaagcgc
     4321 ccgagatcga ccgcccgctg cgccgtatag ccggtctcct cctcgagctc gcggatcgcc
     4381 gccgaggccg ccgcctcgcc ttcctcctcg tcgccgacca ggccggcggg aagctcgagg
     4441 cagcgcgcgc cgatcggcac ccgatattgc tcgacgagga ggacgtgccc ctcgtccacc
     4501 gccaggatca ccgccgcgct gactccgcgg gtccgggaga cgaattccca tgtgccgagc
     4561 ttcttcgcct cgatgaagcg gccgctccag acgacttccg gcgcttcgct cacagctcga
     4621 tcagccggtc cggcaattcg ttgggattgt cgagggtcgg cggcaggcac tgcgccagca
     4681 cctcgccgat cttcccgacc gcgagcgcca tcccctcccc ggctttcccc gacttcacgc
     4741 cctcgaccag ggccgccatc gcctcccccc acacgtccgg ctcgacccgg ccgtggatcg
     4801 cctcgtcggc gacgatctcg gcccggtgct cgagcaggga cagatagagg aggatcccgg
     4861 tccgtccctt ggtccttttc tccgtgccgg cccggaacag ctcgacggcg cggcgccgga
     4921 cccggcgcgt cttggtgctc gccggggtga gagccatccg aagcggcatc caggcgaagg
     4981 cgaagcggac gatcaggaag acgatggcca tcttgacgac gagcagaagc atcagcagcc
     5041 ggaagctcaa ggtcgcgttc cagccgaaga ccagccccag cgcccagtcg atccacccct
     5101 gcgggagcac cgccgcgatc gtgggcacga gcagcatcgc aagcaccgcg taatgaagac
     5161 cgacgtcgtg ataggcgtcg gagcgcgg
//
LOCUS       DASWNU010000309         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_480252, whole genome shotgun sequence.
ACCESSION   DASWNU010000309 DASWNU010000000
VERSION     DASWNU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480252"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGB57_06065"
     CDS             <1..375
                     /locus_tag="VGB57_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HEY0130953.1"
                     /translation="AAHKTARRRFDIKLGPGGLVDLEFAVHTLQLKHNIGLYPHLEDA
                     LEELVAAGLVSGEIDPALRLLTRMLVMFRLVSPASDEPPDETKPLVAAACGLADWNAL
                     LAAHDSARQRVAELWRRVASGE"
     gene            378..842
                     /locus_tag="VGB57_06070"
     CDS             378..842
                     /locus_tag="VGB57_06070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0130954.1"
                     /translation="MLSEGDKVPDVKLASLEGKAISPADFGGQKLVIYFYPKDDTSGC
                     TAEAQAFTALADEFKNAGTWILGVSKDDAKKHVKFIDKFGLKIPLATDSDGSVCEAFG
                     TWVEKSLYGRKYMGIERATFLVDRDGVVKRVWRKVKVPGHAEEVLEAARELP"
     gene            872..1681
                     /locus_tag="VGB57_06075"
     CDS             872..1681
                     /locus_tag="VGB57_06075"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HEY0130955.1"
                     /translation="MLDLPSEWEEALARAEAAKRVAVIGPADSGKSAFVSALAGRRPE
                     CRVIDLDPGQKMIGPPGTVSLGRLSPERRVDRIVFLGSTSVGSFKALAAAAADLARRA
                     RRGFVANTSGYVAGPGAALQAMTLNALRPELVVAIAAPPSFDRLLAKWPAVRLGRSPS
                     ARPKPPGLRRATRQAAFAEALAGACERTLREPAWEPGPPRPTAGGERPVCAVADASGE
                     DRAIGILLSPERLLTRFRGVAGRIRLGRLWAVPGAEGWTLIERLQPAWEPR"
     gene            1681..2676
                     /locus_tag="VGB57_06080"
     CDS             1681..2676
                     /locus_tag="VGB57_06080"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0130956.1"
                     /translation="MRPNLHPRLINGRFGDPGLFVEMLHRREALLFDLGDLSPLSARD
                     LLRVSHVFVTHAHMDHFVGFDALLRVNVGRDKTIRLVGPAGFADRVWHKLQAYEWDLV
                     DRYAADLVFEVTELGADGRTRSARFRFKRAFAREELGDGVAEEGLVATGEGFAVRAAI
                     FEHHGPSVGFAVAEPVHVNVWKNRVDARGLAAGPWLQALKQAILEGRPDEWPVETPAG
                     PAALAGLRDLVSLGPGQKVAYVTDVADSPRNRAAIADLARGADVFFLESCFAAADGKQ
                     ALERAHLTTRAAGEIARAAGVRRLEPFHFSPRYEGEEARMLAEVEEAFTTGSAAR"
     gene            2888..>3203
                     /locus_tag="VGB57_06085"
     CDS             2888..>3203
                     /locus_tag="VGB57_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEY0130957.1"
                     /translation="MFTDFTETLPTAPPTRPPGTAEDWTIPQNWARFGAEEHRVWDVL
                     YARQRRKLSGRAVGAFEQGLDSLLLSRSGIPDFDDLNARLFARSGWTVVAVPGLLPDE
                     VFF"
BASE COUNT          455 a         1095 c         1135 g          518 t
ORIGIN      
        1 gccgcgcaca agacggcaag gaggcggttc gacatcaagc tcggccccgg ggggctggtc
       61 gatctggaat tcgccgtcca cacgcttcag ctgaagcaca atatcggcct ctatccccat
      121 ctcgaggatg cactcgagga gcttgttgcg gccggcctgg tgtccggcga gatcgacccg
      181 gcgcttcgcc tcctgacccg gatgctggtc atgttccgcc tggtctcgcc ggcctccgac
      241 gagccgcccg acgagaccaa gcccctggtc gccgccgcct gcggccttgc cgactggaac
      301 gctttgcttg cggcccacga ttcggcacgg cagagggtgg cggagctgtg gcgccgggtg
      361 gcgtcgggag aatgaagatg ctgagcgaag gcgacaaggt tccggacgtg aagctggcga
      421 gcctcgaggg caaggcgatc agcccggccg atttcggcgg ccagaagctg gtgatctatt
      481 tctaccccaa ggacgacacg tcgggctgca ccgccgaggc gcaggccttc accgccctgg
      541 ccgacgagtt caagaatgcg ggcacctgga tcctcggcgt gtcgaaggac gacgccaaga
      601 agcatgtgaa gttcatcgac aagttcggcc tcaagatccc actcgccacc gattccgacg
      661 gttccgtctg cgaggcgttc ggcacctggg tcgagaaaag cctgtacggc cgcaaatata
      721 tggggatcga gcgcgcgacc ttcctcgtcg accgggacgg cgtggtgaag cgggtgtggc
      781 ggaaggtgaa ggtgccgggg catgccgagg aagtgctgga ggcggcgagg gagctgccgt
      841 aaccaagcag cgcggcgggc gttgaccccc gatgctcgac ctgccgtcag agtgggagga
      901 agcgctcgcc cgagccgagg ccgcgaagcg ggtcgccgtg atcggtcccg ccgactccgg
      961 caagtccgcc ttcgtctcgg cgctggccgg gcgccggccc gaatgccggg tcatcgacct
     1021 cgacccgggc cagaagatga tcggcccgcc cggaaccgtc tcgctcggcc gactgagtcc
     1081 ggagcggcgg gtcgaccgca tcgttttcct gggcagcacg tccgtgggca gctttaaagc
     1141 gcttgccgcg gcggcggcgg atctggcgcg gcgggcccgg cgcggcttcg tcgccaatac
     1201 ctccggctat gtcgccgggc cgggggcggc gctgcaggcg atgaccctca acgctcttcg
     1261 tccggagctt gtcgtcgcca tcgccgctcc gccttccttc gaccgactgc ttgcgaaatg
     1321 gccggcggtg cggctcggcc ggtctccttc ggcgcggccc aagcctcccg gcttgcgccg
     1381 cgcgacaagg caggccgcct tcgcggaagc ccttgccgga gcttgcgagc ggacgttgcg
     1441 cgagcctgcc tgggagccgg ggccgccccg gccgacggcg gggggcgagc gcccggtctg
     1501 cgcggtcgcc gacgcttccg gcgaggaccg ggcgatcggc atcctgctga gcccggagcg
     1561 gctgctgacc cgcttccgcg gcgttgccgg gcgcatccgc ctcggcaggc tctgggccgt
     1621 gccgggggcg gagggctgga ccctgatcga gcgcctccag ccggcgtggg agccgcgctg
     1681 atgcgcccca acctccatcc gcgcctgatc aacggccgct tcggcgatcc cggcctgttc
     1741 gtcgagatgc tgcaccggcg cgaggccctg ctgttcgacc tgggcgacct ctcgccgctc
     1801 agcgcccgcg acctgctgcg ggtgtcccat gtcttcgtca cccatgcgca tatggaccat
     1861 ttcgtcggct tcgacgcctt gcttcgggtg aatgtcgggc gggacaagac gatccggctg
     1921 gtggggccgg cgggcttcgc cgatcgcgtg tggcacaagc ttcaggctta cgagtgggac
     1981 ctggtcgacc gctatgccgc cgatctcgtc ttcgaggtga cggagctcgg cgccgacggc
     2041 cgcacccgct ccgcgcgctt ccgcttcaag cgcgctttcg cacgggagga gcttggcgac
     2101 ggcgtcgccg aggaggggct ggtcgcgacc ggcgagggct tcgcggttcg ggccgccata
     2161 ttcgagcatc acgggccgtc ggtcggcttc gccgtcgccg agccggttca cgtcaacgtc
     2221 tggaagaatc gcgtggacgc ccgcgggctg gcggccggac cctggctcca ggcactgaaa
     2281 caggcgattc tggaggggcg gccggacgaa tggccggtgg agactccggc gggacccgcg
     2341 gccctcgcag ggctgcgcga tctggtcagc ctcggtccgg ggcagaaggt cgcctatgtc
     2401 accgacgtgg ccgattcgcc tcgcaaccgc gccgccatcg ccgacctcgc ccggggcgcc
     2461 gacgtcttct tcctcgaatc ctgtttcgcc gccgccgacg gcaagcaggc gctggagcgg
     2521 gcgcacctca ccacccgggc ggccggggag atcgcccggg cggcgggtgt gcggcggctc
     2581 gagcctttcc acttctcgcc gcgctacgaa ggcgaggagg cgaggatgct ggcggaggtg
     2641 gaggaggcgt tcaccacagg ctcagccgcg cgttgacaaa ccggcctttg taagtcagct
     2701 ttcgaaggat gaagccgatg cgcgcctccg ccgctcacct gaacgcgacg atctccgccg
     2761 cggcttgcct ctattattat tacggaatcg ctcgggcggg accggaatag cacgcgctta
     2821 acaagccagg cttcaaaccg accccgtccg gacctccggg cggggttttt tcatgtggag
     2881 agacccgatg ttcaccgatt tcaccgagac cctgcccacg gcgccgccga cgcgcccgcc
     2941 ggggacggcc gaggattgga cgatcccgca aaactgggcc cggttcggcg ccgaggagca
     3001 tcgggtctgg gacgtcctct acgccaggca gcgccgcaag ctttccggcc gggcggtcgg
     3061 cgccttcgag caaggtctcg actcgctcct cctgtcgcgt tcgggaatcc ccgatttcga
     3121 cgacttgaac gcgcgcctgt tcgcccgctc gggatggacc gtggtggccg tgccgggcct
     3181 gcttcccgac gaggtcttct tcg
//
LOCUS       DASWNU010000310         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_504807, whole genome shotgun sequence.
ACCESSION   DASWNU010000310 DASWNU010000000
VERSION     DASWNU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504807"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..449)
                     /locus_tag="VGB57_06090"
     CDS             complement(225..449)
                     /locus_tag="VGB57_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130958.1"
                     /translation="MAGPASCPKCTGAMERGFIVDQGHGTYTVGDWAAGEPVKSIWTG
                     LKLRGKARLKIATWRCRRCGFLESYAADAR"
     gene            complement(464..904)
                     /gene="sdhD"
                     /locus_tag="VGB57_06095"
     CDS             complement(464..904)
                     /gene="sdhD"
                     /locus_tag="VGB57_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY0130959.1"
                     /translation="MTQDPRGVPAEPAQATGPGLETPLARARGLGSAREGAEHWWNER
                     ASSVAVLLLFVWLIVSLLRLPSLDHRGVTEWLRDPLAAVPMLLLIAATFWHLHMGLKV
                     IVEDYVHDEGNKFLSILLLNFAAVGAAAFAIFAVLKIAFAQSGP"
     gene            complement(901..1299)
                     /gene="sdhC"
                     /locus_tag="VGB57_06100"
     CDS             complement(901..1299)
                     /gene="sdhC"
                     /locus_tag="VGB57_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY0130960.1"
                     /translation="MSRNPNRPLSPHMTVYKWGPHMLVSIVHRITGAGLALVGGAALV
                     WWLVAAASGEEAYESFAGWATSWIGIVIGVGLTWAFFQHTLSGLRHLVMDIGAGFEIE
                     RNKFWAMMTLAGALLLTAALWLYIVIGRGA"
     gene            complement(1388..>1789)
                     /locus_tag="VGB57_06105"
     CDS             complement(1388..>1789)
                     /locus_tag="VGB57_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130961.1"
                     /translation="AAASRDYVPPPHQARVDSVQAAIRSEGPGRRPGLFRPPPERPAR
                     SGREIDLRVAEEVELAARHIEQVGAALVADPAFLHRHGATLQALDLINQNLRHLAAIV
                     AADDKEAAAERISLEELRRRLTRKSIRTIGG"
BASE COUNT          293 a          626 c          620 g          250 t
ORIGIN      
        1 ggcgggcgct cgacccgcta tcccgaccag gaagagcaga agggctccga agccgagcac
       61 ggctgccaca cgcagcaggt tgcgacgaag ccgaaggtcc gccgcgtgca ataacggccg
      121 ctctttgctt gccatcacga ctcgcccccc tgcagcttac cgaaggcgga tcatgtcaca
      181 aggaagcgca tggaccaatc gatcgcgacg ggccaggctg ccccttacct tgcatccgcc
      241 gcgtagctct ccaggaagcc gcagcggcgg cagcgccagg tcgcgatctt gagccgggcc
      301 ttgccgcgca gcttcaggcc ggtccagatg ctcttcaccg gctccccggc ggcccagtcg
      361 cccaccgtgt aggtgccgtg gccctggtcg acgatgaagc cccgctccat cgcgccggtg
      421 catttcgggc agctcgccgg tccggccata tgcgcagccc cggctacggt ccgctttggg
      481 cgaaggcgat cttcaggacc gcgaagatcg cgaaggcggc cgccccgacc gcggcgaagt
      541 tgaggagcag gatcgagagg aacttgttgc cctcgtcatg gacgtaatcc tcgacgatca
      601 ccttgaggcc catgtgcaga tgccagaagg tggcggcgat gaggagcagc atcggaaccg
      661 cggcgagcgg gtccctgagc cactcggtca ctccgcgatg gtcgagcgac ggcagccgga
      721 gcagcgacac gatcagccag acgaacagca ggaggacggc gaccgaggag gcccgctcat
      781 tccaccaatg ctccgccccc tcgcgcgccg agccgaggcc gcgggcccgc gccagcgggg
      841 tctcgaggcc cggcccggtc gcctgggcgg gttccgccgg cactccgcgc ggatcctggg
      901 tcatgctccc ctcccaatca cgatatagag ccagagcgcg gcggtgagca gaagcgcccc
      961 cgccagcgtc atcatcgccc agaatttgtt gcgctcgatc tcgaacccgg cgccgatgtc
     1021 catgacgaga tggcgaagcc cggagagcgt gtgctggaag aaggcccagg taagccccac
     1081 tccgataacg atcccgatcc acgacgtcgc ccagccggcg aagctctcat aggcctcctc
     1141 ccccgacgcg gcggcgacca gccaccagac cagagccgcg cccccgacga gggcgagccc
     1201 cgccccggtg atccggtgaa cgatcgagac cagcatgtgc ggcccccatt tgtagacggt
     1261 catatgcggc gagagcggac ggttggggtt gcgcgacatg gaaagccttc ctgttgctgc
     1321 gcgcttttag gggcaatgcg gggggatgca agggcgggaa ggcctgacgg cacggccggc
     1381 cccttcccta gcctccgatc gtccggatcg acttgcgggt cagccgccgc ctcagctcct
     1441 cgaggctgat ccgctcggcc gccgcctcct tgtcgtcggc ggcgacgatg gcggcgaggt
     1501 ggcgcagatt ctggttgatg aggtcgagcg cctggagcgt ggcgccgtgg cggtggagga
     1561 aggcggggtc ggcgaccagc gccgctccga cctgctcgat atggcgggcg gcgagctcca
     1621 cctcctcggc gacccggaga tcgatctcgc gcccggatcg ggccggccgc tcgggcggtg
     1681 ggcggaacag gccgggcctc cgccccgggc cctccgagcg gatcgccgcc tggacggaat
     1741 cgacccgcgc ctgatgcggg ggcggcacgt agtcgcggct tgcggcggc
//
LOCUS       DASWNU010000311         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_348679, whole genome shotgun sequence.
ACCESSION   DASWNU010000311 DASWNU010000000
VERSION     DASWNU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348679"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1527)
                     /locus_tag="VGB57_06110"
     CDS             complement(211..1527)
                     /locus_tag="VGB57_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY0130962.1"
                     /translation="MRITALKTALAAVIATMDGTLQAAADDNNREFTAEEQTAYDAQV
                     KEAEGLQAQIKREEGLLALRASVAAPVSVPGNPGNPGAGAGTVPATVAEKPEPGAIVG
                     RVAIAIAATGGQDQRAMAAHASSVWGDETGQIVANMEQSTNTKGGYLVDTAYSRDFIG
                     LLRPRVVIRSLGARSVPMPDGNLTMRKQTGSTTAGYVGERSPAPTTGITVGTLSMSAK
                     TLRALVPITNQLLRRAGWGVDTLVRDDLVTSAAIKEDQQFLRGTGSALAPAGLRSLII
                     ASHVLTMTAGPDLVKVTNDLSRLRLKVINANIPMSKCGYIMSPRTSEYLENIRDGNGN
                     KAFPEVAQGKLGNYPIGVTTSVPDNLGAGSNESEIYFGDFEQFLIGDTHQVTLAASTD
                     AAYEEDGEIRSAFSNDETLIRLIEEHDTQLRHDSAFAVLTGVTWGA"
     gene            complement(1547..>2047)
                     /locus_tag="VGB57_06115"
     CDS             complement(1547..>2047)
                     /locus_tag="VGB57_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY0130963.1"
                     /translation="SVHVTGMCCSAAYWIASSAKGGISLDPTGIVGSIGVLISTSYQV
                     EPDSEGRRDLDIASSNAPNKRPDLSTEEGRAQVRQMLDGIEEIFIQTVARGRGVTEAT
                     VRNEFGQGGTRTGKDAKAAGMIDRVEADGLDGAIRRLARSAPPAAPRRTAAANSLKLA
                     RLRAGL"
BASE COUNT          270 a          663 c          749 g          365 t
ORIGIN      
        1 tctcgggctt gtgcacctcg gcgaagccct gcttcaccag gcgcgcctcg gtctcccgat
       61 cgaaaccggc gacctcgtcg acgttgtaga gcgtgccctg ctggcacggc ttgatgaact
      121 tcacggccat aatcggctct ccagaaacgc gaaggggcgc ccgtgggcgc ccctcgcaag
      181 gttggtaggt ggtggtggcc tcaggccggc ttacgcgccc caggtgacgc cggtgagcac
      241 cgcgaaggcg gagtcgtggc gcagctgggt atcgtgctcc tcgatgaggc ggatgagcgt
      301 ctcgtcgttg gagaaggccg accggatctc gccgtcctcc tcgtaggcgg cgtcggtcga
      361 cgcggcgagc gtgacctggt gcgtgtcgcc gatgaggaac tgctcgaagt cgccgaagta
      421 gatctccgac tcgttcgagc ccgcgcccag gttgtccgga acggaggtgg tgaccccgat
      481 cggatagttg ccgagcttgc cctgggcgac ctcggggaag gccttgttgc cgttgccgtc
      541 gcggatattc tcgagatatt ccgaggtccg cgggctcatg atgtagccgc acttcgacat
      601 cgggatattg gcgttgatga ccttcagccg caggcgggaa aggtcgttgg tgaccttcac
      661 cagatccggg ccggccgtca tcgtcaggac gtggctggcg atgatgaggc tgcgaaggcc
      721 ggcgggcgcc agggcgctgc cggtgccgcg gaggaactgc tggtcctctt tgatcgcggc
      781 cgacgtcacg agatcgtcgc ggacgagcgt gtcgacgccc cagccggcac ggcggagcag
      841 ctggttggtg atcggaacga gcgcgcgcag cgtcttcgcc gacatgctga gggtgccgac
      901 cgtgattccg gtcgtcggcg ccggcgagcg ctcgccgacg tagccggccg tggtgctgcc
      961 ggtctgcttc cgcatggtga ggttgccgtc cggcatcggg accgaacgag caccgaggct
     1021 ccggatgacg acgcgcgggc gaagcaggcc gatgaagtcg cggctgtagg cggtatcgac
     1081 gagatagccg cccttcgtgt tggtcgactg ctccatgttg gcgacgatct ggccggtttc
     1141 gtcgccccag acgctcgagg cgtgggcggc catcgcccgc tggtcctggc cgccggtcgc
     1201 ggcgatcgcg atcgcgacac ggccgacgat cgcgcccggt tcgggcttct cggcgacggt
     1261 ggccgggacg gtgccggcgc cggcgccggg gttgcccggg ttgcccggga cactgaccgg
     1321 agcggcgacg gaggcgcgca gcgcgagcag gccctcttcc cgcttgatct gggcctgaag
     1381 cccttcggcc tctttgacct gggcgtcgta ggcggtctgc tcctcggccg tgaactcgcg
     1441 gttgttgtcg tccgcggcgg cctgcagcgt tccgtccatc gtggcgatga cggccgccag
     1501 ggccgtcttc agcgcggtga tgcgcatata tacgtctcct tgggggttag agacccgcgc
     1561 gcagtcgcgc gagcttcagg gaattcgccg cggccgtccg ccgaggcgct gccggagggg
     1621 cgcttcgcgc gagccggcgg atcgctccgt ccaggccatc ggcctcgacg cgatcgatca
     1681 tgccggccgc cttggcgtcc ttcccggttc tggtgccgcc ctggccgaac tcgttgcgga
     1741 ccgtcgcttc ggtcacgccg cggccgcggg ctacggtctg gatgaagatc tcctcgatgc
     1801 cgtcgagcat ctggcggacc tgggcccggc cctcctcggt cgacaggtcc gggcgcttgt
     1861 tcggcgcgtt cgagctggcg atgtccaggt cgcggcggcc ctcgctatcc ggctccacct
     1921 ggtaggacgt ggagatcagc acgccgatcg agccgacgat gccggtcgga tcgaggctga
     1981 tcccgccctt ggccgagctg gcgatccagt aagcggccga gcagcacatg ccggtgacgt
     2041 ggaccga
//
LOCUS       DASWNU010000312         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_194763, whole genome shotgun sequence.
ACCESSION   DASWNU010000312 DASWNU010000000
VERSION     DASWNU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194763"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGB57_06120"
     CDS             <1..435
                     /locus_tag="VGB57_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130964.1"
                     /translation="AAVDHIPRGARVVSFVGVKCGKDWAQTRIEHLPALALVRRDAFS
                     NDQWSMSGAQLLTARFPDGGWFSRDPSQQVLARPCRGERWLTLDQSLALFPRDKFTHV
                     WLISPPRPDPALLRGLRPVWQSGTSVLYRVADPSPILLQEPE"
     gene            432..1466
                     /locus_tag="VGB57_06125"
     CDS             432..1466
                     /locus_tag="VGB57_06125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0130965.1"
                     /translation="MTAPPALSIVVPCYNEEACLAELHGRLTAAARTAVGEDYEIVLV
                     NDGSRDRSWEMMRSLAAVDERLVAINLSRNHGHQLALTAGLDLCSGARILIIDADLQD
                     PPELLSPMLAEMDRQRADVVYAVRRARAGETAFKRGTAKLFYRLLSRLAEIEIPRDTG
                     DFRLMSRRALDALLSLPEQARFIRGMVAWVGFRQVPIAYDRAERHAGQSKYPLSKMVR
                     FALDAVTGFSTAPLRMASHIGLWLVAASALLVAYIAFGFFTGSAIQGWTSLMLVVVVL
                     GAVQMFVLGMIGEYLGRLYMESKRRPLYIVAEIAGHAGGEPTLGHVAHEAETIATSET
                     PGGNGSRPIA"
     gene            complement(1400..2128)
                     /locus_tag="VGB57_06130"
     CDS             complement(1400..2128)
                     /locus_tag="VGB57_06130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0130966.1"
                     /translation="MERIVYDRMAELDSRHWWYRARREILADLIERRIALPDKPRILE
                     IGCGTGHNIVMLKRFGRVDAIEIDPAARAIASQRLGHAVMDAPLPALTGVEDGAYDLV
                     AILDVLEHVEEDREALESIARKLRPGGRILIAVPAHPWMWSAHDVVNHHKRRYTRKTL
                     RGAVAAAGLKLEMMSWFNSLLFPLAAAARLAGKVRGKEDSDDKLPPRPLNALFEKIFG
                     LERYAIGRLPLPPGVSLVAIVSAS"
     gene            complement(2144..2548)
                     /locus_tag="VGB57_06135"
     CDS             complement(2144..2548)
                     /locus_tag="VGB57_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0130967.1"
                     /translation="MKLQGAIPPVFEQVIRFGLVGGFVTLVGGAAYYIPATYYGVDPL
                     LSNLLAYAVAMALGYVLHSTVSFKGHGKRDNTAKRTGRFLIVSLVSLGLNSLWVWSLT
                     DLLEGPTWWPLVPMLFVTPAVTFALNRQWVFG"
BASE COUNT          411 a          957 c          916 g          443 t
ORIGIN      
        1 gccgccgtcg accatatccc gcgcggcgcc agggtcgtgt ccttcgtcgg ggtcaaatgc
       61 ggcaaggact gggcgcagac ccgaatcgag catctccccg ccctcgccct ggtgcggcgc
      121 gacgccttct ccaacgacca atggtcgatg agcggggccc agctgctcac cgcccgcttt
      181 ccggacgggg gctggttctc gcgcgatccg tcgcagcagg tgctcgcccg cccctgccgg
      241 ggggagcgct ggctcaccct cgaccagtcg ctggccctgt tcccgcgcga caaattcacc
      301 catgtatggc tgatcagccc gccgaggccc gacccggcgc tcctgcgcgg gctccggccg
      361 gtctggcaaa gcggcacgag cgtgctgtat cgggtcgccg accccagccc gatcctcctc
      421 caggaacccg aatgaccgcc ccccccgccc tttccatcgt cgtgccctgc tacaacgagg
      481 aggcgtgcct cgccgagctt cacggccggc tgacggcggc ggcgcgaacg gccgtgggcg
      541 aggattatga gatcgtgctc gtcaacgacg gctcgcgcga ccgaagctgg gagatgatgc
      601 gctcgcttgc ggcggtcgac gagcgactgg tggcgatcaa cctgtcgcgc aaccacggcc
      661 accagctcgc ccttacggcg gggctcgatc tatgctcggg cgcgcggatc ctgatcatcg
      721 acgccgacct tcaggatccg ccggagctgc tctcgccgat gctggccgag atggaccgcc
      781 agcgcgccga cgtcgtctat gccgtgcggc gggcgcgggc cggcgagacg gcgttcaagc
      841 gcggcaccgc caagctcttc taccgcctgc tctcccgtct cgccgagatc gagataccgc
      901 gcgacacggg cgatttccgg ctgatgagcc ggcgagcgct cgacgccctg ctcagcctgc
      961 ccgagcaggc gcgcttcatc cgcggaatgg tcgcctgggt cggctttcgc caggtgccga
     1021 tcgcctacga ccgggccgag cggcatgccg gacagagcaa atatccgctg tcgaagatgg
     1081 tccgcttcgc cctcgacgcc gtcaccggtt tctcgaccgc gccgctgcgg atggcgagcc
     1141 acatcggcct ctggctggtc gcggcgtccg cgcttctggt cgcttatatc gccttcggct
     1201 tcttcaccgg cagcgccatc cagggctgga cctccttgat gctcgtcgtc gtcgtgctcg
     1261 gcgcggtgca gatgttcgtg ctcggcatga tcggcgaata tctcggccgg ctctacatgg
     1321 agtcgaagcg tcgcccgctc tacatcgtgg ccgagatcgc cggccacgcc ggcggcgagc
     1381 ccactttagg ccatgtcgct catgaggcgg agacgatcgc caccagcgag actcccggcg
     1441 gcaacggcag ccgcccgatc gcgtagcgct ccagcccgaa tatcttttcg aacaaagcgt
     1501 tgagcggcct cggcggaagc ttgtcgtcgc tatcctcctt cccgcgcacc ttgccggcga
     1561 ggcgggcggc ggcggcgagc gggaagagca ggctgttgaa ccagctcatc atctccagct
     1621 tcagcccggc cgcggcgacg gcgccgcgaa gcgtcttgcg ggtgtagcgg cgcttgtggt
     1681 ggttgacgac gtcgtgggcg ctccacatcc acgggtgcgc cggcaccgcg atcaggatcc
     1741 ggccgccggg ccggagcttg cgggcgatgc tctccagcgc ttccctgtcc tcctcgacat
     1801 gctcgagcac gtcgaggatg gcgacgaggt cgtaggcgcc gtcctcgacg cccgtcaggg
     1861 ccggaagcgg cgcgtccatg accgcgtggc cgagccgctg gctggcgatc gcccgggcgg
     1921 cgggatcgat ctcgatcgcg tcgacccggc cgaagcgctt gagcatgacg atattgtgcc
     1981 cggtgccgca gccgatttcg aggatcctcg gcttgtccgg caaggcgatg cgacgctcga
     2041 tcaggtcggc gaggatctcg cgccgggccc ggtaccacca gtggcggctg tcgagctccg
     2101 ccatgcggtc gtagacgatg cgttccatca gcttccggcc atctcagccg aacacccatt
     2161 gccggttgag cgcgaaggtg acggcgggcg tgacgaacag catcggaacg agcggccacc
     2221 aggtcggccc ctcgagaaga tcggtcaggc tccagaccca gaggctgttc agcccgaggc
     2281 tgacgagcga gacgatcagg aagcgcccgg tgcgcttcgc cgtattgtcg cgcttgccat
     2341 ggcccttgaa gctcaccgtg ctgtggagga cgtaacccag cgccatggcg acggcatagg
     2401 ccaacaggtt ggagaggagc ggatcgacgc cgtaataggt cgccggaatg tagtatgccg
     2461 cgccgccgac cagggtgacg aagccgccca ccaggccgaa gcgtatcacc tgctcgaata
     2521 ccggcgggat cgcgccctgc agcttcatgt ccagcgcgtt tccctctcgc ccgcggcttg
     2581 ccctttgccg tgcaccgata gggctcaaac tcaacaccgg caatggccag gatcgcccgg
     2641 agaaggccat cggctggttg cccgcacaag cgctccgacg cagccgatgg ccttctccgg
     2701 gcgaccgcga agcggcgggg ggggggg
//
LOCUS       DASWNU010000313         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_245517, whole genome shotgun sequence.
ACCESSION   DASWNU010000313 DASWNU010000000
VERSION     DASWNU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245517"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..>2159
                     /locus_tag="VGB57_06140"
     CDS             234..>2159
                     /locus_tag="VGB57_06140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY0130968.1"
                     /translation="MKSLYFLAASAAALSVAAVSLPAPLAAQARQAPAPKKFGSWGVD
                     LGARDTSVKPGDSFFDFANGGWYKTAVIPPDQPVAGIALDTHLLTQAQLRSVVEDSAR
                     NPSTRSARQVGALYASFMDEAGLERLDASPLAADLAAVKAVGSKSDMARLMGATTGTF
                     GSSFFNFGVTPDPKGTNLYTTAIEVGGLGLPDRDYYLTDQFKAQREAYAGHVGRTLEL
                     IGWPDAAVAAQAVVALETRIAEASWSRAEQRDPTKMFKEMTLQALRAHAPEFDWTAFL
                     AGVGAPPVERLIVTQDSAVPKIARIFAEAPLDTLKAWEAFHIADQAAPYLSKRFVENR
                     FAFTRAISGQQQNRARWERGVQLVNGVLGEVLGQEYVRRHFPTSSKARMEEMVRGLQA
                     AMRARIEGAAWMSAETRAAALEKLAKQRVKVGYPSKWRDYSALELRRDDLYGNVKRAG
                     AFTVRYHFDRIGKPVDKEEWMMTPQTVNAYFNPLGNEIVFPAAMLQAPMFDPRADAAV
                     NYGAIGAVIGHEISHGFDDQGRQFDEAGRLRDWWKPQDAARFTAEAGKLADQYSAYEG
                     VPGMKVNGKLTLGENIGDQGGLRIALDAYHSTLKGKKAPAIDGFTGDQRFFLAFAQGW
                     RGKVRDEMMKMILVSDPH"
BASE COUNT          379 a          732 c          713 g          335 t
ORIGIN      
        1 gctcgtctca atgcaccagc tcggatcgtt cgaggcccgc gcttcgatcc ggatctcgaa
       61 aatgggcgag gcccgggtgg taagcttcga cgcgctcctc gccgcgacac ggctttagcg
      121 acgaaccggg tgcggccgcc aagccgctca taacgtaacg aaaaatcccg cgctgctgac
      181 cgggcgtccg gcagccgacg gccaagcctt gcccgaaaga aaggaacgat ccgatgaaga
      241 gtctctattt cctcgcagcc tcggcggcgg ccctgtccgt ggccgccgtc tccctcccgg
      301 ccccgctcgc cgcccaggcc cggcaggcgc cggcgccgaa aaaattcgga agctggggcg
      361 tcgatctcgg cgcccgcgat acctcggtga agccgggcga cagcttcttc gattttgcca
      421 acggcggctg gtacaagacg gccgtgatcc cccccgacca gccggtcgcc ggcatcgctc
      481 tcgataccca tttgctcacc caggcgcagc tgcgctcggt ggtcgaggac agcgcccgca
      541 acccctcgac gcgaagcgcc cggcaggtcg gcgcccttta tgccagcttc atggacgagg
      601 ccggactgga aaggctcgac gcctccccgc tcgccgccga tctcgccgcc gtcaaggcgg
      661 tcggctcgaa gagcgacatg gcgcggctga tgggcgccac caccggcacc ttcggctcca
      721 gcttcttcaa tttcggggtg acgcccgatc ccaagggcac caatctctac acgaccgcaa
      781 tcgaagtcgg cgggctcggc cttccggatc gggattatta tctcaccgac cagttcaagg
      841 cgcagcgcga ggcctatgcc ggccatgtcg gccggacgct ggagctgatc ggctggcccg
      901 atgccgccgt cgcggcgcag gccgtggtcg cgctcgagac caggatagcc gaggcgagct
      961 ggtcgcgggc cgagcagcgc gatccgacga agatgttcaa ggagatgacg ctccaggccc
     1021 tgcgcgcgca tgcgcccgaa ttcgactgga ccgccttcct cgccggagtc ggcgctccgc
     1081 ccgtcgagcg gctgatcgtc acccaggaca gcgcggtgcc gaagatcgcc aggatattcg
     1141 ccgaggcgcc gctcgacacg ctcaaggcct gggaggcctt ccatatcgcc gaccaggccg
     1201 cgccctatct ctcgaagcgc ttcgtcgaga acaggttcgc tttcacccgg gcgatcagcg
     1261 gccagcagca gaaccgcgcg cgctgggagc gcggcgtgca attggtcaac ggcgtcctcg
     1321 gcgaggtgct cggccaggaa tatgtccgcc gccacttccc gacttcgtcc aaggccagga
     1381 tggaggagat ggtgcggggc ctccaggcgg cgatgcgggc tcggatcgag ggcgcggcgt
     1441 ggatgagtgc ggaaacccgc gccgcggcgc tcgagaagct ggcgaaacag cgcgtgaagg
     1501 tcggctatcc cagcaaatgg cgcgattatt ccgcgctcga gctgaggcgg gacgatctct
     1561 acggcaacgt caagcgcgcc ggcgccttca ccgtccgcta ccatttcgac cgcatcggca
     1621 agcccgtcga caaggaggag tggatgatga ctccgcagac ggtgaacgcc tatttcaacc
     1681 cgctcggtaa cgagatcgtc ttcccggccg cgatgcttca ggcgccgatg ttcgatccca
     1741 gggcggacgc tgcggtgaat tatggcgcga taggcgcggt gatcggccac gagatcagcc
     1801 acggcttcga cgatcagggc cggcagttcg acgaagccgg ccggcttcgc gactggtgga
     1861 agccgcagga tgccgcccgc ttcacggcgg aggcggggaa gctcgccgat cagtacagcg
     1921 cctatgaagg cgttcccggc atgaaggtga acggcaagct gacgctcggc gagaatatcg
     1981 gcgaccaggg cgggctgcgg atcgcgctcg acgcctatca ttccaccttg aagggcaaga
     2041 aggccccggc gatcgacggc ttcaccggcg accagcgttt cttcctcgcc ttcgcccagg
     2101 gttggcgcgg caaggtccgg gacgagatga tgaagatgat cctcgtctcc gatccgcac
//
LOCUS       DASWNU010000314         5325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_400708, whole genome shotgun sequence.
ACCESSION   DASWNU010000314 DASWNU010000000
VERSION     DASWNU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400708"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGB57_06145"
     CDS             <1..213
                     /locus_tag="VGB57_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130969.1"
                     /translation="RSHAMTEKPISDLHPSDQDHVPADQGHVPPDQGRASSLYDEQLQ
                     RQQQEKMLLSRVEQRLDLLAPAKDDD"
     gene            complement(288..740)
                     /locus_tag="VGB57_06150"
     CDS             complement(288..740)
                     /locus_tag="VGB57_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEY0130970.1"
                     /translation="MFNSHDVTVLNSLIETTIDSVDGYRHSAGEATNEQFAEIFRNRA
                     QEREEVVGLLRRRVTELGGTSEDDGSLLAKAHRQFLSLRDAVTGSDDKSVIAEVDRGE
                     SYLNGKWEAALADEGVSAETQQLLRQCYQSVRSGHDQWEQMNKSLSAG"
     gene            855..1070
                     /locus_tag="VGB57_06155"
     CDS             855..1070
                     /locus_tag="VGB57_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130971.1"
                     /translation="MPEEESERGKPATFDPKSGEVSGSGSGAGGGGNPREDYDQDPMA
                     GAGAEPPHGPRSAEDVKDRPIDPDEGV"
     gene            1072..1260
                     /locus_tag="VGB57_06160"
     CDS             1072..1260
                     /locus_tag="VGB57_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130972.1"
                     /translation="MTGKGHPLPGSDEARHNEIPEEVAEEKGRGDDSKPVVQPGRDAV
                     NPDGERYRYDDNGRAEKI"
     gene            1400..1954
                     /locus_tag="VGB57_06165"
     CDS             1400..1954
                     /locus_tag="VGB57_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130973.1"
                     /translation="MANQNNQDGGFSGSELQQSQSGGGSGEFGGNRDQQQQSGQQGGG
                     ASSSDIDQAGGSSGGGGYGSGQNQQFHQGQQDEGGQSGLGGGGDLRQGGGSSEGQSRG
                     EAFDEAQGGGRGNSDFQQDQQGGQFQQDQQGGTFGGQEELQQQQGGQSQREGGSDIEF
                     QQDQQSHQDRGQGKSGIEFEGDQS"
     gene            2090..2917
                     /locus_tag="VGB57_06170"
     CDS             2090..2917
                     /locus_tag="VGB57_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130974.1"
                     /translation="MADNSSGQDGSGASKGKGGSGGVLPDALKEAGARAAELAQNPVA
                     RSMLAAGLVTAAAALTANARVRKSVRDAGRDALDTAEAAADSASKIGAAIVTAATDAV
                     RRLLAGAEESGGTEGGGAGSSSGRKSGGKSGGAKASAKSSGAKSSGAKSGASKSTGTR
                     AKSAGSGGGARGKAASASGSGGKAASASGSRGKAAGAASGAKKTASSGRGKAAGAASG
                     GGSKSGSGASGSTGSSGKGASSGGGRGGGRKKAGAGASASGGSSGAGGDGGSEGSGG"
     gene            complement(3025..4350)
                     /locus_tag="VGB57_06175"
     CDS             complement(3025..4350)
                     /locus_tag="VGB57_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY0130975.1"
                     /translation="MIGKRGGLGRSETRLWLFGTTAALVMTAAGTAEAAGFYLQEQSV
                     RGWGRANSGEVADQGPASLWWNPAAIAGATEAEAAFGATGILPSGRVSDSGTLIDRPG
                     APPEPVGGLPRLRDPILRGIAPNNAFALPLGERIALGLAVTAPFSFTSQYDPSGWQRY
                     SAIRTRLLTLDFQSSVAVKATDWLSVGAALNVQYSDAWLSNALPNLAPGSPDARLRLT
                     GNGWDLGWSAGLQLRPSERVSIGIAYKSAIEHKTRGPIEVTGLTGALAARNTSGPATV
                     RFTTPWQLLAGARVGITEGLTLNAQAVHFGWSKFDILDISGPVNNAIVQNYKDTWSLA
                     VGFDQSLGEQLTLRAGVQIDPTPTRDANRDPRVPDGDRVNYNVGATYRMSERITLDAA
                     ASLTDVEASPINRDERFYAGTPAQTDVLTEGRVDRQRVLILALGGRMSF"
     gene            4593..>5325
                     /locus_tag="VGB57_06180"
     CDS             4593..>5325
                     /locus_tag="VGB57_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY0130976.1"
                     /translation="MNHHFGLPTSDETIPMDVAVEGRAGALPLQHRERPAGTRLFDLG
                     QLEVRWEAELGTLWTHMTPVDRPNFNRAMLRDFQRWQAEIGREFADPREGLKYLVLGS
                     RFPGIFNLGGDLELFARFISAGDREGLVRYGRDCVSILHNNMRRLELPIVTVALVQGD
                     ALGGGFEAVLSFNVIVAEKGSRFGLPEIAFGLFPGMGAHSLLSRKIGLARAEAMMLSN
                     RLYTAEEMYEMGIVHILAEPGCGEQA"
BASE COUNT          908 a         1767 c         1874 g          776 t
ORIGIN      
        1 cgttctcacg cgatgaccga gaaaccgatt tccgacctgc acccttcgga ccaggaccac
       61 gtccctgcgg accagggcca cgtccctccg gaccagggcc gcgcctcctc cctctacgac
      121 gagcagctcc agcgccagca acaggagaag atgctgctca gccgggtcga acagcgcctc
      181 gacctcctgg cgccggcgaa ggacgacgac tgacgtcgga ataagcttgg cgccaccaat
      241 ggtcccctcc ccacgcgctt gcgcgcgcgg ggaggctgga ccggtcttca gccggcgctg
      301 aggctcttgt tcatctgctc ccactggtcg tgcccggagc ggaccgactg gtagcattgg
      361 cggagcagct gctgggtctc ggccgacacg ccctcgtcgg cgagtgcggc ttcccacttg
      421 ccgttaaggt agctctcgcc gcggtcgacc tccgcgatca ccgacttgtc gtcgcttccg
      481 gtgacggcgt cgcgcagcga caggaactgg cggtgcgcct tggcgagcag gctgccgtcg
      541 tcctccgaag tgccgccgag ctcggtgacc cggcggcgca gaaggccgac gacttcctcg
      601 cgctcctggg cacggttgcg gaagatctcg gcgaattgct cgttggtcgc ttcaccggcg
      661 gaatggcggt agccgtcgac gctgtcgatc gtggtttcga tgaggctgtt gaggacggtt
      721 acgtcatggc tgttgaacat gggcttctcc tggcgttggg ggtcgcggtc tcaacccgcg
      781 gagggtcgtt aagggtccgt gccggaacgg gattatgcgc caggacgttc aatggccgca
      841 aaaggagcac ggacatgccc gaagaggaaa gcgagcgggg aaagcccgcg accttcgacc
      901 ccaagagcgg cgaggtcagc ggctccggct cgggcgcggg cggcggcggc aacccgcggg
      961 aggattacga tcaggatccg atggcgggcg ccggcgccga gccgccgcac ggcccccgct
     1021 cggcggagga cgtgaaggat cgcccgatcg acccggacga gggagtttga gatgacgggc
     1081 aagggccatc cgctcccggg cagcgacgaa gcgcggcaca acgagatccc cgaggaggtg
     1141 gccgaggaga aaggccgcgg cgacgacagc aagccggtcg tccagccggg ccgcgacgcc
     1201 gtcaaccccg acggcgaacg ctaccgctac gacgacaacg gaagggcgga gaagatatag
     1261 ggccgaataa atcctccccg cgaaaacgcg ggaggattca ggttcagccc gtccgcgcac
     1321 cagtccggcg ctccacccgg agccgatggc cggacttaac gttgatcaag ctcccagtat
     1381 ttcagccagg agcatgatca tggccaatca gaacaatcag gacggcggtt tttcgggcag
     1441 cgagctgcag cagtcgcagt ccggcggcgg cagcggcgag ttcggcggca atcgcgatca
     1501 gcagcagcag agcggccagc agggcggcgg cgcctccagc tccgacatcg accaggccgg
     1561 cggcagcagc ggcggcggcg gctatggcag cggccagaac cagcaattcc accagggcca
     1621 gcaggacgaa ggcggccaaa gcggcctcgg cggcggcggc gaccttcgcc agggcggcgg
     1681 aagctccgag ggccagtcgc gcggcgaggc gttcgacgag gcgcagggcg gcggccgcgg
     1741 caattccgac ttccagcagg accagcaggg cggacagttc cagcaggacc agcagggcgg
     1801 cacgttcggc ggccaggagg agctccagca gcagcagggc ggccagtccc aacgcgaggg
     1861 cggctccgac atcgagttcc agcaggacca gcagtcgcac caggatcgcg gccagggcaa
     1921 gagcgggatc gagttcgagg gcgaccagag ctaaggacgc tcccgatcga acgactggaa
     1981 gggccgtccc gccggggacg gcccttcttg ccgtgggcgg cgccgaaagg aactactcgc
     2041 cgccccgcgg gtttgttacg catcagttac aagatcacag gagaccgaca tggcggataa
     2101 cagttcgggc caggacggct caggcgcctc gaaaggcaaa ggcggttcgg gcggagtcct
     2161 tccggacgct ctcaaggagg ccggcgcgcg cgccgccgag ctggcccaga acccggttgc
     2221 ccgctcaatg ctcgccgccg gcctcgtcac cgccgcggcg gcgctcaccg ccaatgcccg
     2281 ggtacgcaaa tcggtccggg acgccggccg cgacgcgctc gacacggccg aggcggccgc
     2341 cgacagcgcc agcaagatcg gcgccgcgat cgtcaccgcc gccaccgacg ccgtccggcg
     2401 cctgctggcc ggcgcagagg aaagcggcgg caccgaaggc ggcggcgcgg gctcctcgag
     2461 cgggcgcaag agcggcggca aatcgggcgg cgcgaaggcg tcggcgaaat cctccggcgc
     2521 gaaatcctcc ggcgccaagt ccggcgcgtc caaatcgacc gggacccggg ccaagagcgc
     2581 gggatcgggc ggaggcgctc gcggcaaggc ggcttccgct tccggcagcg gcggcaaggc
     2641 ggcttccgct tccggcagcc gcggcaaggc ggccggagcg gcttccggcg ccaagaagac
     2701 ggcttcgagc gggcgcggca aggccgccgg ggcggcttcg ggcggcggca gcaagagcgg
     2761 ttcgggtgcg agcggctcca ccggcagctc gggcaagggc gcctcgagcg gcggcggccg
     2821 tggcggcggc cgcaagaagg ccggcgctgg cgcttcggca tccggcggca gcagcggcgc
     2881 cggtggagac ggcggctccg agggttcggg cggctaagcc cccgtcaccc cggcgaaagc
     2941 cggggcctca gggaggctcg ccgacactct acaccatggg gtcccggctt tcggcgggat
     3001 gacggatcgc cggaatgacg gagcctagaa gctcatcctg ccgcccagcg cgaggataag
     3061 tacgcgttgc ctgtcgactc ggccctccgt cagcacgtcg gtctgggcgg gggtgccggc
     3121 gtagaagcgc tcgtcgcggt tgatcgggct ggcttcaacg tcggtaaggc tggccgcggc
     3181 atccagagtg atgcgctcgc tcatccggta ggtcgctccg acattgtagt tgacccggtc
     3241 gccgtccggc acgcgcggat cgcgattggc gtcccttgtg ggggtcggat cgatctgtac
     3301 tccggcccgt aatgtgagct gctcgccgag cgactggtcg aagccgacgg cgaggctcca
     3361 cgtatccttg taattttgga cgatggcgtt gttgaccggg ccggagatgt cgaggatgtc
     3421 gaacttgctc cagccgaaat ggaccgcctg ggcattcaga gtgagcccct cggttatgcc
     3481 aacgcgcgca cccgccagga gctgccaggg cgtcgtgaag cggacggtcg ccgggccgct
     3541 ggtgttgcgc gcggcgagcg cgccggtgag cccggtgact tcgatcggcc ccctggtctt
     3601 gtgctcgatc gccgatttgt aggcgattcc gatcgacacc cgctccgacg gacgaagctg
     3661 aaggccggcg ctccagccca ggtcccagcc attgccggtg agccggagcc gggcgtcggg
     3721 agatccgggc gccaggttcg gaagggcgtt ggaaagccag gcgtcgctat actggacgtt
     3781 cagcgccgcg ccgacgctga gccagtcggt ggccttcacg gccacggacg actggaagtc
     3841 gagcgtcaga aggcgcgtgc gaatcgcgga atagcgctgc cagccggacg gatcgtattg
     3901 gctggtgaag ctgaacggcg cggtgacggc gaggccgagg gcgatccgct cgccgagcgg
     3961 cagcgcgaac gcattgttcg gagcgatgcc ccggaggatg gggtcgcgaa gacggggcaa
     4021 tccgccgacc ggctccggcg gagctcccgg ccggtcgatc aaggtgccgg agtcgctgac
     4081 ccgtccgctt ggaagaatgc cggtcgcccc gaaggccgct tcggcctcgg tggcgccggc
     4141 gatggccgcc gggttccacc acagcgacgc cgggccctga tcggcgacct ctcccgaatt
     4201 cgctcggccc catcccctaa ccgattgctc ctgaagatag aatccggcgg cctcggcagt
     4261 gccggcggcg gtcataacca gcgccgcagt cgtcccgaaa agccacagtc gcgtctccga
     4321 gcgaccgagc ccgccacgtt tgccgatcat catgcgtccc ccaacttgat ccgaacgtgc
     4381 gccccgcaca gctacgggtt agaaacgaaa gctttgtttc tcaacctttt taatttgcat
     4441 ccgaaatgga catttgacgg tcctcatccg gatcaatacg agcccccggc gcaacaccta
     4501 atcggttaac gctcggaacg tgcgcaggcc caattgttcc acagccgctt atatgcttac
     4561 ctctgcgtac accaaatgcc tggggcaaga tgatgaatca ccatttcggt cttccgacgt
     4621 ccgacgagac gatcccgatg gacgtcgccg tggaggggcg cgcgggggcg cttcccctgc
     4681 agcatcgcga gcggcccgcg ggcacacgcc tgttcgacct tggccagctc gaagtccgct
     4741 gggaggccga gctcggcacc ttgtggactc acatgacacc ggtcgaccgg cccaatttca
     4801 accgcgccat gctgcgcgat tttcaacgct ggcaggcgga gatcggccgc gagttcgccg
     4861 atcctcggga gggtctcaaa tatctggtgc tcggctcgcg tttcccgggc atttttaacc
     4921 tgggtggcga tctcgagctc ttcgcccgct tcatctcggc gggcgaccgc gagggactcg
     4981 tccgctacgg acgcgattgc gtctcgatcc tccacaacaa catgcgccgg ctcgagcttc
     5041 cgatcgtcac cgtcgccctg gtccagggcg acgccctcgg cggcgggttc gaggcggttc
     5101 tgagcttcaa cgtcatcgtc gccgagaagg gatcgcgctt cggccttccc gagatcgcgt
     5161 tcggcctttt tcccggaatg ggggcccaca gcctgctgtc gcgcaagatc ggcctcgccc
     5221 gcgccgaggc gatgatgctg tccaaccgcc tctacacggc agaggagatg tacgagatgg
     5281 gaatcgtcca catcctcgcc gaaccgggct gcggcgagca ggcgg
//
LOCUS       DASWNU010000315         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_116590, whole genome shotgun sequence.
ACCESSION   DASWNU010000315 DASWNU010000000
VERSION     DASWNU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116590"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGB57_06185"
     CDS             complement(<1..387)
                     /locus_tag="VGB57_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEY0130977.1"
                     /translation="MSNNLFRSLALVSAVAIIPAASASLAAVDVDTYRELDQFMSVFE
                     RVRSEYVEQVDDKTLIRGAIDGMLASLDPHSSYMDERGYQALMTTTDGNYGGLGLNVT
                     MEDGVVKVVAPIEDSPGFKAGIKAGDY"
     gene            complement(611..1801)
                     /locus_tag="VGB57_06190"
     CDS             complement(611..1801)
                     /locus_tag="VGB57_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0130978.1"
                     /translation="MDGHGKSVPGTAHGRWRIWCLAPIFLTFAVALSLFAGDLAIAQA
                     PPTLAQAQREAAAAKRRSDLLERQAQRATGEAARANAAAAALAARIEAAEADITAAEA
                     RIALIERLSAAQRARLAASQGSLIRLTAALQTMARRPPALALIQPGSLDEVVHVRALL
                     ASSLPAIRARTAGVRSEIARGEALRRQAREAVVQLGSGRRDLQQRRIALARFEQAQRR
                     RSASLMQSALRESDRALAFGEEARDIATLARTQAFQASLERRLSELPGPAPRPGATPA
                     RPRPPYYVLPVEGRLLTGTGEISDAGVHARGLTFATAAGSAAVAPRAGRVAYAGPYRG
                     YGEVVIIDHGGGWTTTMTNLSGLAVKRGDRIRARQPLGRTGSGEVTVELRKDGRPFPI
                     TLLL"
     gene            complement(1806..2228)
                     /locus_tag="VGB57_06195"
     CDS             complement(1806..2228)
                     /locus_tag="VGB57_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY0130979.1"
                     /translation="MKLHILARGKIGRSPEAELVERYLKRVSWPAQVTELPESGGRLA
                     APAENSVTILLDEKGETLGSLDLARRLERWRDEGRREARFLIGGADGFTDEDRSSADL
                     LLSFGRATWPHLLARAMLAEQLWRATSIRANHPYHREG"
     gene            complement(2343..2750)
                     /gene="rsfS"
                     /locus_tag="VGB57_06200"
     CDS             complement(2343..2750)
                     /gene="rsfS"
                     /locus_tag="VGB57_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY0130980.1"
                     /translation="MPANAPAPPTGGANEKIEALHNLVLRSLDDDQAQDVVSIPLAGK
                     SAIADHMVIASGRSTRQVASMAQKLGERIKQELGRNVRVEGLPVADWVLIDADDVIIH
                     IFRPEVRSFYNLERMWAFEGDAGAPPAPATAAG"
     gene            complement(2760..3413)
                     /locus_tag="VGB57_06205"
     CDS             complement(2760..3413)
                     /locus_tag="VGB57_06205"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY0130981.1"
                     /translation="MDAAVIRTGLFGGSFNPAHRGHRRVSAAAIRALGLDEMWWLVSP
                     GNPLKPVQGMAPLPARLASARRLARGLPVRATAIEAELGTRYTVDSLDALVRLYPDRR
                     FIWLMGEDNLAQFHLWRHWRKIARTVPIAVVSRPGYDGAAHAARAMGWLRRFVQPADQ
                     ARHWTEWRPPALVLLRLPLDPTSATGIRAADPNWHQSEAYSFNPRALRDGVTRRLVT"
     gene            3467..>3802
                     /locus_tag="VGB57_06210"
     CDS             3467..>3802
                     /locus_tag="VGB57_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0130982.1"
                     /translation="MAADDKSCTLPDQDVREFIRETFACSDEIAGYVFMRGAVRAYPP
                     HATIVHEGEKSAAAYLLTQGQAHALLYSVEGQVVLLCDYRPGALFGALGELDPAPAEA
                     ESVAVEAARS"
BASE COUNT          572 a         1242 c         1418 g          570 t
ORIGIN      
        1 gtagtcgccg gccttgattc cggccttgaa gccggggctg tcctcgatcg gagcgacgac
       61 cttcaccact ccgtcctcca tcgtgacgtt gaggccgagg ccgccgtaat tgccgtcggt
      121 ggtggtcatc agcgcctgat agccgcgctc gtccatgtag gagctgtgcg ggtcgaggct
      181 ggcgagcatt ccgtcgatcg cgcctcggat cagggtcttg tcgtcgacct gctcgacata
      241 ttccgagcgg acccgctcga agacgctcat gaactggtcg agctcgcgat aggtgtcgac
      301 gtcgaccgcg gcgagcgagg cgctcgcggc cgggatgatc gccaccgcgc tgaccagagc
      361 gagcgagcgg aagagattgt tggacattcg ggaatcctgc ctgggtgttc gagacgcttg
      421 taccgccttt tttagcgggc gaaaagcaac cctgcctgaa cgagcaatat caacttgcct
      481 ccccatgcgc agcatgggga gggggacgcc gcgtagcagg gtggaggggt aatggcgccg
      541 acctgtgccg gttacccctc caccaggctt cgcctggtcc ccctccccat ctggcgatgg
      601 ggaggcaaaa tcaaagcagc aacgtgatcg gaaacggcct tccgtccttg cggagctcga
      661 ccgtgacctc gcccgagccg gttcggccga gcggctgtcg ggcccggatc cggtcgccgc
      721 gcttgacggc gaggccggaa aggttggtca tcgtcgtcgt ccagccgccg ccatgatcga
      781 tgatcaccac ctcgccatag ccgcgatagg gcccggcata agcgacgcgt ccggcgcggg
      841 gggccaccgc cgcggagccg gcggcggtcg cgaaggtgag gccgcgggca tggactccgg
      901 cgtcggagat ctcgccggtg ccggtgagga ggcggccctc gacgggaagg acgtaataag
      961 gcgggcgcgg gcgggccggc gtcgcacccg ggcggggcgc cggccccgga agctcggaga
     1021 ggcggcgctc gaggctggcc tggaaggcct gggtgcgggc cagggtggcg atatcgcgcg
     1081 cctcctcgcc gaaggccagg gcgcggtccg attcgcgcag ggccgactgc atcaggcttg
     1141 ccgagcgccg tcgctgcgcc tgctcgaagc gggcgagtgc gatccggcgc tgctgaaggt
     1201 cgcggcggcc cgagccgagc tggaccaccg cctcgcgggc ctggcggcga agcgcctcgc
     1261 cgcgcgcaat ctcggagcga actccggcgg tccgggcccg gatggcgggg agcgacgagg
     1321 cgagcagggc gcggacgtgg acgacctcgt ccagggagcc gggctggatc agcgccagcg
     1381 cgggcgggcg gcgggccatg gtctgcaggg cggcggtgag ccgtatgagc gagccctggc
     1441 tggcggcgag gcgcgcgcgc tgggcggcgc tgaggcgctc gatcagggcg atccgggcct
     1501 cggccgcggt gatgtcggcc tccgccgcct cgatccgggc ggcgagggcg gcggcggccg
     1561 cattggctcg ggcggcttcg ccggtggcgc gctgagcctg gcgctcgagc agatcggagc
     1621 ggcgcttggc ggccgccgct tcccgctggg cctgggcgag cgtcggcggc gcctgggcga
     1681 ttgcgaggtc gccggcgaat agcgagaggg cgacggcgaa cgtgaggaaa atcggtgcca
     1741 ggcaccaaat tctccagcgt ccgtgagcgg tgcccggcac cgattttccg tggccatcca
     1801 tttcctcatc cttccctgtg atagggatgg ttggcacgga tcgaggtggc gcgccagagc
     1861 tgctcggcga gcatcgcccg agcgaggaga tggggccagg tggcccgtcc gaaggagagt
     1921 aggaggtcgg ccgacgacct gtcctcgtcc gtaaagccgt cggcgccgcc gatcaggaag
     1981 cgcgcctcgc gccggccctc gtcgcgccag cgctcgagtc gacgggcgag gtcgagcgag
     2041 ccgagcgttt cgcctttctc gtcgaggagg atggtgactg agttctccgc cggggccgcg
     2101 aggcgaccgc cgctttcggg caattcggtg acctgggccg gccaggagac ccgcttcaga
     2161 tagcgctcga ccagctccgc ttcggggctc cggccgatct tgccgcgggc gaggatgtgg
     2221 agcttcattc agctaaagca cgtcattgcg aggagcgaag cgacgaagca acccagcggg
     2281 cctcggaggc cgctctggat ggcttcgctc gcaatgacgg cctaggggcg cggcaacggg
     2341 tcctatcccg ccgccgtggc aggcgcgggc ggtgctccgg catccccctc gaacgcccac
     2401 atgcgctcga gattgtagaa gctgcgcact tccgggcgga agatgtggat gatgacgtcg
     2461 tcggcgtcga tcagcaccca gtcggcgacc ggaagcccct cgacccggac attgcggcca
     2521 agctcctgct tgatccgctc gcccagcttc tgcgccatcg aggcgacctg gcgggtcgag
     2581 cggccggacg cgatcaccat atgatcggcg atcgcggact tgcccgccaa tgggatggag
     2641 acgacgtcct gggcctggtc gtcgtcaagc gaccgaagca cgagattgtg cagggcttcg
     2701 atcttctcat tcgctccgcc cgtgggcgga gccggtgcgt tagcgggcaa acggcctcct
     2761 caagtcacga gccgccgcgt tacgccgtcg cgcagggccc gggggttgaa cgaataagct
     2821 tccgattggt gccagttggg atcggcggca cgaatgcctg tggcggaggt cggatcgagc
     2881 ggcaatctca agagcacgag cgccggcggt ctccactccg tccagtgccg agcctggtcc
     2941 gcgggttgga cgaagcgccg cagccagccc atcgcgcgag ccgcgtgggc ggcgccatca
     3001 tatcccggac gcgaaacgac cgcaatcgga accgtgcggg cgatctttcg ccagtgccgc
     3061 cagaggtgga actgggcgag attgtcctcg cccatcagcc agatgaagcg gcggtccgga
     3121 tagagccgga ccagggcgtc gagggagtcg acggtgtagc gggtgccgag ctcggcctcg
     3181 atcgcggtgg cccgcaccgg aaggccccgg gcgaggcgcc gggcggaggc gagccgggcg
     3241 gggaggggcg ccatgccctg gaccggcttc agcgggttgc ccggcgagac cagccaccac
     3301 atttcatcga gcccgagggc gcggatcgcc gccgcgctca cccggcggtg gccgcggtga
     3361 gcggggttga acgagccgcc gaaaaggccg gtgcggatca cggcagcgtc gatagcatgg
     3421 ccccggcgct tgccaagcgc ttccgcctcg tgctgttctt cgcgccgtgg ccgccgacga
     3481 caaaagttgc accctgccgg atcaggacgt tcgcgaattc attcgcgaga ctttcgcctg
     3541 ctccgacgag atcgccggct atgttttcat gcgcggggcg gtgcgcgcct atccgccgca
     3601 cgcgaccatc gtccatgaag gcgagaaatc ggcggccgcc taccttctca cccaggggca
     3661 ggcccacgcc ttgctgtaca gcgtcgaggg gcaggtggtc ctcctctgcg actatcgccc
     3721 cggcgccctg ttcggagcgc tgggcgagct cgaccccgcg ccggcggagg ccgagagcgt
     3781 cgcggtcgag gcggcgcgaa gc
//
LOCUS       DASWNU010000316         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_400789, whole genome shotgun sequence.
ACCESSION   DASWNU010000316 DASWNU010000000
VERSION     DASWNU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400789"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..284)
                     /locus_tag="VGB57_06215"
     CDS             complement(126..284)
                     /locus_tag="VGB57_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY0130983.1"
                     /translation="MQQAHISALQHRHADIEARIEEESQRPLPDTATLARLKKEKLWI
                     KEEIAGLH"
     gene            437..649
                     /locus_tag="VGB57_06220"
     CDS             437..649
                     /locus_tag="VGB57_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY0130984.1"
                     /translation="MEAIDGNAVPAASRLVRLRQEHRDLDSAIEALRVAPSSDQLQLA
                     RLKKRKLRLKDEIAQLEDQLIPDIIA"
     gene            856..1314
                     /locus_tag="VGB57_06225"
     CDS             856..1314
                     /locus_tag="VGB57_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEY0130985.1"
                     /translation="MPEPRLPLTAKLVASLYTEAMLLADEARSYFDMSGRADRDSLDP
                     IDRVGFSCESLKVTTRLMHVIAWLLTQRAVEAGELSASQARRPARRLGDAPDSDEALL
                     PRLPEAAVALIHSSRDLYARVKRLDDAPAPGAEPQASPARSLMSRLERSF"
     gene            complement(1322..2290)
                     /locus_tag="VGB57_06230"
     CDS             complement(1322..2290)
                     /locus_tag="VGB57_06230"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEY0130986.1"
                     /translation="MTLPAVLQRLRLPAIGAPLFIVSNPDLVIAQCKAGIVGSFPALN
                     ARPQSLLDEWLHRITEELAAWDRDNPDRPAAPYAVNQIVHRSNDRLQQDMATCARWKV
                     PIVITSLGAIVEVNQAVHGWGGIALHDIINDRFARKAVEKGADGVIAVAAGAGGHAGT
                     LSPFALVQEIRQWFEGPLILSGAIATGRAILAAQAMGADLGYIGSPFIATAEANAAEA
                     YKQGICDGRAADIVYSSLFTGVHGNYLRASIAASGLDPDNLPEGDLKTMDFGGGESSK
                     KAWRDIWGSGQGIGAIDRVRPAGEYVAGLIRQYEEAKSELRAKAEL"
BASE COUNT          349 a          769 c          784 g          406 t
ORIGIN      
        1 ggttctatca cgctccgtca ttgcgagcga agcgaagcaa tcccagcgct gagtgggacg
       61 cgctcgccgc ggctgggctg ccgcgtcgct acgctcctcg caatgacggt gggaaagcgc
      121 cgcccctaat ggaggccggc aatctcttcc ttgatccaga gcttctcttt cttgagcctg
      181 gcgagggtgg ccgtatccgg cagcggccgc tgactttctt cttcgatccg cgcttcgatg
      241 tccgcgtggc ggtgttgcaa tgccgagata tgcgcctgtt gcattccctt ctccttggct
      301 gtgctgggcg gggtctcaac gtctggaggc gtggcctgtc tcccgtcgat cggatggccg
      361 tcgcaatatg tacgacaggc cgttaatgga ctatctatag cgcgtttcaa tcggcttgat
      421 aagcgcctgg aggggcatgg aagcgatcga cggaaacgcc gtcccggcgg cctcgcggct
      481 ggtccggctg cgccaggagc atcgcgacct ggactcggcg atcgaggcgt tgagagtcgc
      541 gccgtcgtcc gaccagctcc agctcgcccg gctgaagaag cgcaagctcc ggctcaagga
      601 cgagatcgcc cagctcgagg accagctcat cccggacatc atcgcttgaa ttcctccccg
      661 cgatttcgcg gggaggggga ccgcacgaag tgcggtggag gggttcttga gggctggaag
      721 aacccctcca ccatgcttcg catggtcccc ctccccggcg aatgccgggg aggatttatg
      781 tatcgcgccg gcacatctcc cccttcacca tctttgcccg tggcggcgcg gtcgcctctg
      841 tggcagcatg agcggatgcc cgaaccgcgc cttccactga ccgccaagct ggtcgcttcg
      901 ctctacaccg aggcgatgct gctcgccgac gaggcgcgct cctatttcga catgagcggc
      961 cgggccgacc gcgactcgct ggacccgatc gaccgggtcg gcttctcctg cgagtcgctg
     1021 aaggtcacga cccggctgat gcacgtcatc gcctggctcc tgacccagcg cgcggtcgag
     1081 gcgggcgagc tgagcgcaag ccaggcgcgc cgccccgccc gccgcctcgg cgacgccccc
     1141 gacagcgacg aggcgcttct gcccaggctt ccggaagcgg cggtcgcgct gatccattcc
     1201 agccgcgacc tctatgcccg ggtgaaaagg ctcgacgatg cgcccgctcc gggcgcggag
     1261 ccgcaggcga gcccggcgcg gagcctgatg agccggctgg agcggtcttt ttaagggacg
     1321 attacagctc ggccttcgcc ctcagctccg acttcgcttc ctcatattgc cggatcagcc
     1381 cggcgacata ttcccccgcc ggccttacgc ggtcgatggc gccgatgccc tggcccgagc
     1441 cccagatgtc gcgccaggcc tttttcgagc tctcgccgcc gccgaaatcc atcgtcttga
     1501 ggtcgccttc gggcagattg tcggggtcga ggccggaggc ggcgatcgag gcgcgcaggt
     1561 aattgccgtg gaccccggtg aacaggctcg aatagacgat gtccgcggct cgcccgtcgc
     1621 aaatgccctg cttgtaggct tcggccgcat tggcctcggc ggtggcgatg aagggcgagc
     1681 cgatatagcc gaggtcggcg cccatggcct gcgcggcgag aatggcccgc ccggtggcga
     1741 tcgcgcccga caggatcagc ggcccttcga accactggcg tatctcctgg acgagcgcga
     1801 agggcgacag cgtgccggca tggccgccgg cgccggccgc gaccgcgatc actccgtccg
     1861 cgcccttctc caccgccttg cgggcgaagc ggtcgttgat gatgtcgtgg agggcgatcc
     1921 cgccccagcc gtgaaccgcc tggttgacct cgacgatcgc gcccaatgag gtgatgacga
     1981 tcggcacctt ccatctggcg caggtcgcca tgtcctgctg gagccggtcg ttggaacggt
     2041 ggacgatctg gttgaccgcg tagggcgcgg ccggccggtc gggattgtcc cggtcccagg
     2101 cggcgagctc ctcggtgatg cggtgaagcc attcgtcgag caggctctgc ggccgcgcgt
     2161 tcagggccgg aaaggagccg acgatcccgg ccttgcactg ggcgatgacg aggtcggggt
     2221 tggagacgat gaagagcggg gcgccgatcg ccggaaggcg aaggcgctgg aggactgcgg
     2281 gcaaggtcat tgcgccgcct taacggaa
//
LOCUS       DASWNU010000317         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_400841, whole genome shotgun sequence.
ACCESSION   DASWNU010000317 DASWNU010000000
VERSION     DASWNU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400841"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB57_06235"
     CDS             <1..277
                     /locus_tag="VGB57_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0130987.1"
                     /translation="KAGVLGMALPIARDLMNEGIRVNTILPGVFETPMVAMMPQNVKD
                     ALAAQVPFPKRLGRAEEYARLAVFMAENNYLNGEYVRLDGAIRMAPR"
     gene            388..699
                     /locus_tag="VGB57_06240"
     CDS             388..699
                     /locus_tag="VGB57_06240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0130988.1"
                     /translation="MRVAAMLERGAAEDRRRFMRHPVSFGAGLASSNDRPGAPVVVVD
                     LSTHGCGIEVGGHCEVGARVWLKLPGLESWPSRIAWAEGSRAGLSFDRPLHQAVVERY
                     A"
     gene            complement(840..1595)
                     /locus_tag="VGB57_06245"
     CDS             complement(840..1595)
                     /locus_tag="VGB57_06245"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY0130989.1"
                     /translation="MFLKGKTALVTGSTSGIGLAVARALAAEGARVTLNGFGDPAEIG
                     KLRDELGAGYSDADLTRPEAIEAMMAEAGPVDILVNNAGMQHVAPIDEFPVAKWDLLI
                     ALNLSAAFHTIRLALPGMKSKGWGRIVNTASAHSLVASPNKAPYVASKHAVAGLTKAV
                     ALETATAGVTVNCISPGYVWTPLVENQIPDTMKARGLTREQVMNDVLLAAQPTKKFVT
                     VDQVAALALFLCRDEAAAITGANLSMDGGWTAA"
     gene            1787..2722
                     /locus_tag="VGB57_06250"
     CDS             1787..2722
                     /locus_tag="VGB57_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY0130990.1"
                     /translation="MRLGGYRTSDNVRDQGQGGGGFGFGGGGGGGGGCLFALLPMVLS
                     RFGIGGVLILLLGYCALSYMGGGGGMGVGGSGTTVAPQQGTGATNDPKQACSTEPAKR
                     FSCQVLASTEDTWAKLFAAKKQSYQPAGFRFYGGSGDSGCGAAQSAMGPFYCPTDNNI
                     YLDTSFFQEMQTKLGASGDFAQAYVIAHEVGHHIQNLTGVLEQASSQQRRVSSREGNA
                     IQVRVELQADCYAGVYAANARNEQGQAVMEPGDVEEGLRAAQAIGDDTLQRQAQGRVV
                     PEAFTHGSAAQRTAWLRRGLETGDPAACDTFSGAV"
     gene            complement(2892..>3221)
                     /locus_tag="VGB57_06255"
     CDS             complement(2892..>3221)
                     /locus_tag="VGB57_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130991.1"
                     /translation="DLWDRASWMGEAPPGRIAIPVSHQDQIVVPPPGVRVLAGNAFTP
                     FGMLEYGDAPAISMQFHPEFEPEYAQALIEHRRARVPDPDAAIASLERPNDQALVGEW
                     IRRFLRA"
BASE COUNT          510 a         1033 c         1157 g          521 t
ORIGIN      
        1 caaggcgggc gtgctcggaa tggcgctgcc catcgcccgc gacctgatga acgaggggat
       61 ccgggtcaac accatccttc cgggcgtgtt cgagaccccg atggtggcga tgatgcccca
      121 gaacgtgaag gacgcgctcg cggcgcaggt gcccttcccc aagaggctcg gccgggcgga
      181 ggaatatgcg cggctggcgg tgttcatggc cgagaacaat tatctgaacg gcgaatatgt
      241 ccggctcgac ggagcgataa ggatggcgcc gagataggcc ggagccctac cctcgcaaaa
      301 ctacgtggaa ggcgcccgcg gtaaggagtc gtttaccctt tccggccgaa cattccggct
      361 ccgcaccgac ttgaagggag gatcggcatg cgagttgccg ccatgctcga gaggggagcc
      421 gccgaggacc ggcggcgttt catgcgccat ccggtcagct tcggcgccgg cctcgcctcc
      481 tccaacgacc ggccgggcgc tccggtggtc gtggtcgatc tttccaccca tggctgcggg
      541 atcgaggtgg gcggccattg cgaggtcgga gcccgggtct ggctcaagct ccccggcctc
      601 gaaagctggc cgtcgcggat cgcctgggcg gagggcagcc gcgccggcct gagcttcgac
      661 cggccgcttc accaggcggt ggtggagaga tacgcttaat catcctcccc aagcttgctc
      721 gggagggggc cgggcgaagc ctggtgaagg ggtagttggc gcggatgccc ggattcctgc
      781 gccagttacc cctccaccac ccttcgggcg gtcccctcca tctgcgatgg ggaggatttt
      841 caagccgccg tccagccgcc gtccatgctg aggttcgcgc cggtgatggc cgccgcctcg
      901 tcgcggcaga ggaacagggc cagcgccgcg acctggtcga cggtcacgaa cttcttggtg
      961 ggctgcgcgg cgaggaggac gtcgttcatg acctgctcgc gggtgaggcc gcgggccttc
     1021 atcgtgtcgg ggatctggtt ctcgacgagc ggagtccaga catagccggg gctgatgcag
     1081 ttgacggtca cgcccgcggt ggcggtctcc agcgccaccg ccttggtgag gccggcgacg
     1141 gcgtgcttgg aggccacgta aggcgccttg ttggggctcg cgacgaggct gtgcgccgag
     1201 gcggtgttga cgatccggcc ccagcccttc gacttcatcc ccggcaaggc gaggcggatg
     1261 gtgtggaagg cggcgctgag gttgagggcg atcaggaggt cccatttggc caccgggaat
     1321 tcgtcgatcg gggcgacatg ctgcatcccg gcattgttga cgaggatgtc gacggggccg
     1381 gcctcggcca tcatcgcctc gatcgcctcg ggccgggtca ggtcggcgtc gctgtagccg
     1441 gcgccgagct cgtccctgag cttcccgatc tcggcggggt cgccgaagcc gttgagggtg
     1501 acccgggcgc cctcggcggc gagggcgcgg gcgacggcga ggccgatgcc cgaggtggag
     1561 ccggtgacga gggcggtctt gcctttgagg aacatgcttg cactccgggg agggagcggc
     1621 cgtcattgcg aggagcgcag cgacgaagca atccagtttc gcgcggtcgg cccgactcag
     1681 ctggattgct tcgctgcgct cgcaatgacg gtcgtcgtct gcgcggccca gatttgcaac
     1741 ctagagcaat gccgaagcat tgtccggccg caacagggag agcggtatgc gtcttggcgg
     1801 ctatcggacc agcgacaatg ttcgcgacca gggacagggc ggcggcggct tcggcttcgg
     1861 cggcggcggc ggcggtgggg gcggatgcct cttcgcgttg ttgccgatgg tgctcagccg
     1921 cttcggaatc ggaggagtgc tcatcctcct gctcggttat tgcgcgctca gctacatggg
     1981 cggtggcggc gggatggggg tcggcggctc cgggaccacg gtcgctccgc agcaggggac
     2041 cggtgccacg aacgacccga agcaggcctg ctcaaccgag cccgccaagc gcttctcctg
     2101 ccaggtgctg gccagcaccg aggacacctg ggccaagctg ttcgccgcca agaagcagag
     2161 ctatcagccc gccggcttcc ggttctacgg cggcagcggc gattccggct gcggggctgc
     2221 ccagtccgcc atggggccct tctactgtcc gaccgacaac aacatctatc tcgacaccag
     2281 cttcttccag gaaatgcaga cgaagctcgg cgcctccggc gatttcgctc aggcctatgt
     2341 catcgctcac gaagtcgggc accacatcca gaatctgacc ggagtgctcg agcaggcgtc
     2401 gagccagcag cggcgcgtgt cgagccgcga gggcaacgcg atccaggtcc gtgtcgagct
     2461 gcaggccgat tgctatgccg gcgtctatgc cgccaacgcc aggaacgagc agggccaggc
     2521 agtgatggag cccggcgacg tcgaggaagg gcttcgcgcc gcccaggcga tcggcgacga
     2581 cacgcttcag cgccaggccc agggacgggt cgtgcccgag gccttcaccc acggcagcgc
     2641 ggcgcagcgc acggcgtggc tgaggcgcgg gctggagacc ggcgatccgg cggcctgcga
     2701 tacgttctcg ggcgcggttt gaatctgcct ccccatcgcc agatggggag ggggaccacc
     2761 gaaggtggtg gaggggtaga aggcgctgac gaaggaggtt cttgagtgag ccttccgatc
     2821 ctgagccaat tacccctcca ccatgcttcg catggtcccc ctccccaccg cttcgcggca
     2881 gggaggcaag tctaagcccg caagaagcgc cttatccact cccccaccag cgcctggtcg
     2941 ttcgggcgct ccagcgacgc gatggcggcg tcgggatcgg gcacccgggc ccggcggtgc
     3001 tcgatcaagg cttgagcgta ttcgggctcg aactcggggt ggaactgcat cgagattgcc
     3061 ggcgcatccc catattcgag catcccgaac ggggtgaagg cattgccggc cagcacccgg
     3121 acgccgggag gcgggaccac gatctgatcc tgatgcgaga cgggaattgc gatccggccg
     3181 ggcggcgcct cgcccatcca cgacgcgcgg tcccacagat c
//
LOCUS       DASWNU010000318         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_220845, whole genome shotgun sequence.
ACCESSION   DASWNU010000318 DASWNU010000000
VERSION     DASWNU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220845"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1237)
                     /locus_tag="VGB57_06260"
     CDS             complement(35..1237)
                     /locus_tag="VGB57_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY0130992.1"
                     /translation="MRIIATGMLVAMAAVFLGANAYDEAYPWLGFVKAFAEAAMVGGL
                     ADWFAVTALFRHPLGLPIPHTAIIPRNKDRIGATLASFLRDNFLTPKVVARRMRNVDI
                     AGAIGRFLANPPPEGRLRQGGSRLIADLLEALDQERLGGMVKSAAASRMRTLEVSPLI
                     GQTLEAAITDERHIPILDGIVTWAGRTLDANEDLIRKMVHDRANLIMRLAGLDEKLAD
                     GIVDGLRRLTIDMAIDPAHPLRAKAEEALAALAWDMRFDPEMQARVEGWKNEIIAYKA
                     VTDWLGGLWENSRAALLRAARDPEAAMAGRFGEALKQLGETLQQDGRLNKAINQFARR
                     MVVGTVATYGSGIVTLVSETIRGWDARTVTGRLENAVGRDLQYIRINGTIVGGLVGLL
                     IHTAEVLA"
     gene            complement(1411..>3346)
                     /locus_tag="VGB57_06265"
     CDS             complement(1411..>3346)
                     /locus_tag="VGB57_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0130993.1"
                     /translation="ITLNGLSLLALSLVAGVLVDDAIVEIENIVRHMRMGKSAYQASI
                     DAADEIGLPVVATTFSIVAVFLPVAMMGGIIGQYFISFGLTIVITVLISLAVARLITP
                     MIAAYFLKAHGQAKHGEGWLMDKYLGLLRWTLRNRWKSVGVGFLCFVAQIGVFMMLPS
                     AFQPETDEDTVQVAIEMPPGSTLDQTRAIADRAATLVRGQGDVESAFASVRAGASTIY
                     VSLKDRGEGRERTSTDFERQVGPLLQGIPDARVTFRAQNSIGGRPIAVLLSSSDPAKL
                     QQAALRLVEDMRKRPELVAPRIAGDMRRPEITITPRLNLMADMGVTTSAMSQAIRTAT
                     QGEIDQASARFSLSDRQVPIRVALQEDSRRNLSTIENMPVPTTTGGSVPLKVVASISF
                     GAGPAQIQRFNQERRILVGADLKPGVVDKEAIIESLPILQNLPEGVERARSGQQREEE
                     KLMSEFMGALISGTFLVFAVLVLLYKRLMAPFVNMGSLLLAPLGGFLALWATGHAVSM
                     PVLIGILMLFGIVAKNSILLLDFTIVEMAKGVPKDEAIIDAGHKRAQPIVMTTMAMVA
                     GMVPTALSLEGDASWRAPMAVVVIGGLLLSTLLTLVLVPASFSLADSFEKWLAPKVGR
                     IITYKGPKDHRPAPAEQPAE"
BASE COUNT          539 a         1097 c         1174 g          536 t
ORIGIN      
        1 gctgcaacag ccaaacgcgg caggtccggc gccgtcaggc gagcacctcc gcggtgtgga
       61 tgaggaggcc gacgaggccg ccgacgatgg tgccgttgat ccggatatac tggaggtccc
      121 tcccgaccgc attctcgagc cgtcccgtta ccgtccgggc gtcccagccg cggatcgtct
      181 cggaaaccag ggtgacgatg ccgctgccgt aggtggcgac ggtgccgacg accatccggc
      241 gggcgaactg gttgatcgcc ttgttgagcc gcccgtcctg ctggagggtc tcgcccagtt
      301 gcttgagcgc ctcgccgaag cgcccggcca tcgccgcctc gggatcgcgg gcggcgcgga
      361 gcagagcggc gcggctgttc tcccacaggc cgccgagcca gtcggtcacc gccttgtagg
      421 cgatgatctc gttcttccag ccctcgaccc tggcctgcat ctcgggatcg aagcgcatgt
      481 cccaggccag agcggcgagc gcctcctcgg ccttggcgcg gaggggatgg gcgggatcga
      541 tcgccatgtc gatggtgagc cggcggaggc cgtcgacgat cccgtcggcg agcttctcgt
      601 cgagcccggc gaggcgcatg atcagattgg cgcgatcgtg caccatcttg cggatcagat
      661 cctcgttggc gtcgagtgtg cggcccgccc aggtgacgat cccgtcgagg atcgggatgt
      721 gccgctcgtc ggtgatcgcc gcctcgagcg tctggccgat cagcggcgag acttcgagag
      781 tccgcatccg ggaggcggcc gccgacttga ccattccgcc gagacgctcc tggtcgagcg
      841 cctccagaag gtcggcgatg agcctggacc cgccctgccg gaggcgcccc tccgggggcg
      901 ggttggcgag gaagcggccg atcgctccgg cgatgtcgac gttgcgcatg cgccgggcga
      961 cgaccttggg ggtgaggaag ttgtcgcgga ggaacgaggc gagcgtcgcc ccgatccggt
     1021 ccttgttgcg cgggatgatc gcggtgtggg gaatgggcag gccgagcggg tggcggaaca
     1081 gggcggtgac cgcgaaccag tcggcgaggc cgccgaccat ggccgcctcg gcgaaggcct
     1141 tgacgaagcc gagccaggga taagcctcgt cataggcgtt ggcgcccagg aagacggccg
     1201 ccatcgccac aagcatcccc gtggcgatga tgcgcatgcc ccgcaggctc tgcgcggggg
     1261 gaacgaatcc tccggaatat gcgggtccga cggggttcat ggccatctca atagccgggc
     1321 cggcacttaa gttcactccc gcccccggca agggccgtct cttcatcccc gatcatttcg
     1381 caccggaccg cgcgtctgag cgcgggcgca tcactccgcg ggctgctccg ccggtgccgg
     1441 acggtggtcc ttgggtccct tgtaggtgat gatccgtccg accttcggcg ccagccactt
     1501 ctcgaagctg tcggcgaggc tgaacgaagc cggcaccagg accagggtca ggagcgtcga
     1561 gagcaaaagg ccgccgatca ccaccaccgc catcggcgct cgccagctgg cgtcgccttc
     1621 cagcgacagc gccgtcggca ccattccggc caccatcgcc atcgtcgtca tgacgatcgg
     1681 ctgggcgcgc ttgtggcccg cgtcgatgat cgcctcgtcc ttggggacgc ccttggccat
     1741 ctcgacgatg gtgaaatcga gcagcaggat cgagttcttg gcgacgatcc cgaacagcat
     1801 caggattccg atcagcaccg gcatcgatac ggcgtgccct gtcgcccaaa gggccaggaa
     1861 cccgcccagg ggcgcgagca gaagggagcc catgttcacg aacggcgcca tcaggcgctt
     1921 gtagagcagc accagcaccg cgaagaccag gaaggtgccg gagatgaggg ctcccatgaa
     1981 ctcggacatg agcttctcct cctcccgctg ctggccggag cgggcgcgtt cgacgccttc
     2041 gggcaggttc tggaggatcg gcagcgattc gatgatcgcc tccttgtcga cgacgcccgg
     2101 tttgagatcc gcgccgacca ggatccggcg ttcctggttg aaacgctgga tctgcgccgg
     2161 accggctccg aagctgatcg aggcgacgac cttgagcggc acggatccgc cggtcgtcgt
     2221 cggcaccggc atgttctcga tcgtcgagag gttgcggcgc gaatcttcct ggagcgcgac
     2281 ccggatcggc acctgccggt cggagaggga gaagcgggcg ctcgcctggt cgatctcgcc
     2341 ctgggtggcg gtgcggatcg cctggctcat cgccgaggtg gtgacgccca tgtcggccat
     2401 caggttgagc ctcggggtga tcgtgatctc cgggcgcctc atgtcgccgg cgatgcgcgg
     2461 cgcgaccagc tcggggcgct tgcgcatatc ctcgaccagc ctgagcgccg cctgctggag
     2521 cttggccggg tcggagctgg aaagcaggac cgcgatcggc cggccgccga tgctgttctg
     2581 ggcccggaag gtgactcgcg cgtccggaat gccctggagg aggggcccga cctgccgctc
     2641 gaaatcggtg ctggtccgct cccgcccctc gccgcgatcc ttcagcgaca cgtagatggt
     2701 cgaagctccg gcccggaccg aggcgaacgc cgattcgacg tccccctggc cgcgcaccag
     2761 cgtcgccgcg cggtcggcga tggctcgggt ctggtcgagg gtcgagccgg gcggcatctc
     2821 gatcgcgacc tgcaccgtat cctcgtcggt ctccggctgg aaggccgacg gcagcatcat
     2881 gaagacgccg atctgcgcga cgaagcagag gaaaccgacg ccgacggact tccaccggtt
     2941 gcgaagcgtc cagcgcagca ggccgagata tttgtccatc agccagcctt cgccatgctt
     3001 ggcctggccg tgcgccttga gaaaataggc cgcgatcatc ggggtgatca ggcgcgcgac
     3061 ggcgaggctg atcaggacgg tgatgacgat ggtgaggccg aagctgatga aatattggcc
     3121 gatgatcccg cccatcatcg cgacgggcag gaagacggcg acgatcgaga aggtggtggc
     3181 gaccaccgga aggccgatct cgtccgcagc gtcgatcgac gcctgatagg cggatttgcc
     3241 catcctcatg tgccggacga tattctcgat ctcgacgatg gcgtcgtcga ccagcacgcc
     3301 ggcgaccaag ctcaaggcga gcagggaaag cccgttgagc gtgatc
//
LOCUS       DASWNU010000319        10204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_271623, whole genome shotgun sequence.
ACCESSION   DASWNU010000319 DASWNU010000000
VERSION     DASWNU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271623"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VGB57_06270"
     CDS             complement(<1..1309)
                     /locus_tag="VGB57_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEY0130994.1"
                     /translation="MDIAGPLFGAVDFIMREAALFAATGFLILGLSDLAVDLLWLARR
                     AARLGRPLPSAADLPAPARPGRLAIFVPAWDEAAVIGDMLRGAVSAWGEGDWRLYVGT
                     YCNDPATIAAATAVARAEPRVRVVTGAEAGPTTKGDCLNRLWLALLADESAEGLRAKA
                     IVLHDSEDVVHPAELRLFDSLIERFDLVQLPVLPLIDPNSRLLGGHYADEFAESHGKE
                     MVVRGALGAGLPSAGVGCAFSREALSRLAAGRGGLPFDSDSLTEDYELGLRIAEAGGR
                     RAFVRVRGEDGGIVATREYFPGTLSASVRQKARWMTGIALSGWDRLGWSGGFAERWMR
                     LRDRQSLLAAILLGAGYLAMALWLALKVREGSGGDGPAPLPALLSGLVTANLGLLVWR
                     LAVRAGFTTAAYGWREGLRAVPRMAIGNLVTMLAAASALSRYRA"
     gene            1613..2512
                     /locus_tag="VGB57_06275"
     CDS             1613..2512
                     /locus_tag="VGB57_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY0130995.1"
                     /translation="MRWIIAAALAGWFFLAPAPARAWGQGGHYTVCEIGYLNLSPRAK
                     AEVDRLVALDGRFASFTETCTFPDNPVSRASEHYSNYLRSQRRIGPGCPGGRPCVLGA
                     IGSDLAILRSGSATDAQKAIAILHIGHWYGDLHQPLHISFADDRGGNSIKAPGPCSGN
                     FEASLHSVWDTCVVERRIFSPGTDRLARARAAAAILNGTITGGQRRSWVRSSPWQWAG
                     ESYKVTIAGRTGYCVRRRGACWYSDSSAEFAKDDPQRTLTVDDAYLDRAKPIVETRLR
                     RAGIRLADALNRTFDPGFDGWRS"
     gene            2509..2712
                     /locus_tag="VGB57_06280"
     CDS             2509..2712
                     /locus_tag="VGB57_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130996.1"
                     /translation="MKLRALLIAAAATLALGPASLGPCLIGTAAAQGFACCKVCRAGK
                     ACGDSCIARDKTCHKGKGCACDG"
     gene            2846..3247
                     /locus_tag="VGB57_06285"
     CDS             2846..3247
                     /locus_tag="VGB57_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130997.1"
                     /translation="MIAAGERFACTPEKVWDGDGPIHCAEGPKVRLAGIAAREMDGSC
                     SPGHPCPDASAEAARDALAGLLGRTTGSASTGHLLVEGPALQCLSEGSAKGSRTAAWC
                     TSPTAGDLNCAMVASGTVAKWERYWRGHSCR"
     gene            3309..4586
                     /gene="nhaA"
                     /locus_tag="VGB57_06290"
     CDS             3309..4586
                     /gene="nhaA"
                     /locus_tag="VGB57_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY0130998.1"
                     /translation="MVEPVARPRSALRDFLKSEAAGGIVLMAAAALALIVANSPFAET
                     YRHLLHLETGPVLSPRLGPMTLHLWINDGLMAVFFLLVGLEIKREFVDGRLSTWDRRR
                     LPVIAAAVGMAVPAAIYLAFTAGADGLSRGWAIPAATDIAFAIGVLALLGSRAPTSLK
                     LFLTTVAIADDLGAVAIIALAYTASLDTLALGAAAAILFVMYAMGKGGVRPLWPYLIG
                     AGLLWYAVLLSGVHATVAGVLAAAAIPIVVTPGAPDSAASPLHRLEHGLSPWVAFLIV
                     PLFGFANAGVSLEGVGLERLFAPLPLGIAAGLFLGKQLGIFGAVWLTVRFGLASRLRG
                     ATWLQVWGVAMLCGIGFTMSLFIGALAFPGDPARVEEAKLGILAGSFVSALGGYLVLR
                     LAPGHRRHQEEEALQEREIESDGDVDGLEERKA"
     gene            4583..5866
                     /locus_tag="VGB57_06295"
     CDS             4583..5866
                     /locus_tag="VGB57_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0130999.1"
                     /translation="MKWLYLFAAVLLAGCVSAGPARDSLDSIARDYVKLQLAIGEKEE
                     GYIDAYYGPPEWQAEAKTGADTPAALAQRAGVLTVRLKSLADDRLQPMERRRRDFLLA
                     QLRAASTRLRMMQGEKLSFADEAEGLFGVRPELKPLAAYDPVLARLETLVPGTGTLAE
                     RVEAFQNRFTIPKERLQPVFDAAIAECRRRTLGHVAMPANEAFTLEFVTGKSWSGYNW
                     YKGNATSLIQVNTDLPIRIGRAVDLGCHEGYPGHHVYNALLETKLAKGRGWVEFTVYP
                     LYSPQSFIAEGSANYGIDLAFPGDERVRYETQVLYPLAGLSPADALAFQALQQSTKDL
                     AGARFTIAREYLEGRMDKEQAIAAIRKYQLVSRARAEQSLAFTEQYRSYVINYGLGED
                     MVRAYVEAAGPGQPERWAAMEKILSEPTLPKELVR"
     gene            5866..6870
                     /locus_tag="VGB57_06300"
     CDS             5866..6870
                     /locus_tag="VGB57_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0131000.1"
                     /translation="MRVFWDERQRLHAPAQELHNGAFAPFAEHCGRVDSMLAALGATH
                     SVADRGEDPLLRIHPRPYLDFLKSAFADWKAAGRPGDASGYVWPVVRRRPLDLDRIDA
                     RLGRYSMDASTPIAEGTWESAYWAAQTALSGLDAVLAGERSAFALCRPPGHHAGADYM
                     GGYCYLNNAAVAAEAGRAAGIGRIAILDVDYHHGNGTQDIFLERGDIFFASIHADPRT
                     DYPFYWGHSDERGAGEGEGATLNLPLPRGTARRDYEAALDRALEVIAGFGAGLLICSF
                     GADTFTGDPICSFALETADYEPVARRIASLGLPTLIVMEGGYAVDALGANVAGFLSGF
                     "
     gene            complement(7045..9054)
                     /locus_tag="VGB57_06305"
     CDS             complement(7045..9054)
                     /locus_tag="VGB57_06305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY0131001.1"
                     /translation="MKSLSAILLLTAAAPALAAGYTPVSDAALGPILARPKSASEVKA
                     MCDRRIAAIGSLQSKVEAMPLSTPPATLIAAYDDLYNLAVSAAFAEPPLIKDTNPDAA
                     IRAAAEDCVQRTNQMVTRFQMSRPVYERLQAVERAGVAPELRYTLQRQLDNFRRAGVA
                     KDGATRTRIEALQNEITETGIEFDRNINSDKSVVKAKPQELAGVPQDYLDRHKPGPDG
                     LVEIRMTGAEIAPVLQYADSAPLRKRVQTAWLNRAYPANDAVLKRLFAQRAELASLLG
                     YPNFAAYDLANRMARDPVRTKAFVDQIAAAARPVGEKEAARLLARLRKDDPKLAGLGS
                     WDTGYALRQVRKETFDVDPAVVRTYFSFDKVRTGIFSLTEDLFGVQIRPWATEVWSPE
                     VRAYELVEKGQAIGRFYLDMHPRDNKYTHAAMFPIRIGIKGRVLPAAALLTNFPEGLM
                     EHGQVETFLHEFGHLLHWLFAGQRGLAMQNFGEIENDVIEAPSTLLEEWVWDADTLAK
                     FATDAQGRPIPRTLVDKMVAGRNFGRAFGTMSQLGLASVALDYYSADMADADLTRRFN
                     ETYGRYALAPNPEGSHMQASFGHLSGYGASYYTYQWSEALAADLLSRFRKAGLRDPAT
                     ARAYRDLVLAPGGSASMNDLARKFLGRDWSVDAYRKELEAGGAPN"
     gene            complement(9129..10058)
                     /locus_tag="VGB57_06310"
     CDS             complement(9129..10058)
                     /locus_tag="VGB57_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY0131002.1"
                     /translation="MKTLVLVEHENGHLKDATLSAVTAASKLGEVHLLVAGEGVGGVA
                     EAASKIAGAGKVHVADGAAYAHQLAENVAPLAAGLMQGHDAFVAASTTTGKNVAPRVA
                     ALLDVMQISDVIAIEGEDTFQRPIYAGNAIATVKSADAKKVITVRGTAFEKAAAEGGS
                     GEIEAVPAGEESVLSSFVGAEIAASERPELTSAKIIVSGGRALGSSEQFHAFIDPLAD
                     KLGAAVGASRAAVDAGYAPNDYQVGQTGKIVAPEVYIAVGISGAIQHLAGMKDSKLIV
                     AINKDEDAPIFQVADFGLVGDLFKLVPELTEKL"
     gene            complement(10058..>10204)
                     /locus_tag="VGB57_06315"
     CDS             complement(10058..>10204)
                     /locus_tag="VGB57_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0131003.1"
                     /translation="QKTPADYGVDTTPRLATLGVREPSKREAGIKVQSVDELIDRLRT
                     VGVI"
BASE COUNT         1556 a         3514 c         3504 g         1630 t
ORIGIN      
        1 gcgcccggta gcgggacaaa gcggaggccg ccgcgagcat cgtcaccagg ttgccgatcg
       61 ccattcgcgg cacggcgcgc agtccctccc gccagccgta agccgccgtg gtgaagccgg
      121 cgcggacggc gaggcgccaa acaagcagcc ccagattggc cgtgaccaga ccggacagaa
      181 gagcgggcaa gggcgcgggg ccgtcgccgc cggagccctc ccggaccttg agcgccagcc
      241 aaagggccat ggccagatag ccggcgccga gcaggatcgc ggcgagcagc gactgccggt
      301 cgcgaagccg catccagcgt tcggcgaagc cgccgctcca gccgagccgg tcccagcccg
      361 aaagggcgat tccggtcatc cagcgcgcct tttgccggac cgaggcggag agggtgcccg
      421 ggaaatattc gcgggtggcg acgatccccc cgtcttcccc gcggacccgc acgaaggcgc
      481 gccgcccgcc cgcctcggcg atcctgaggc cgagctcgta atcctcggta aggctgtccg
      541 aatcgaacgg caggccgccc ctgccggcgg caagtcggct gagggcctcg cgggaaaagg
      601 cgcacccgac gccggccgag gggaggccgg cgccgagcgc gccgcgcacg accatttcct
      661 tgccgtgcga ttcggcgaac tcgtcggcat aatggccgcc gagcaggcgc gaattcggat
      721 cgatgagggg cagcaccgga agctggacga ggtcgaaacg ctcgatcagg gagtcgaaca
      781 gccggagctc ggcggggtgg acgacgtcct ccgagtcgtg gaggacgatc gccttcgccc
      841 gcagtccctc ggcggactcg tcggcgagca gggccagcca cagccggttg aggcaatcgc
      901 ccttggtcgt ggggccggct tcggctcccg tcaccaccct caccctgggc tccgcccgag
      961 cgacggcggt cgcggccgcg atcgtcgcgg gatcgttgca ataggtgccg acatagaggc
     1021 gccagtcgcc ttccccccag gccgagacgg cgccgcgaag catgtcgccg atcaccgccg
     1081 cctcgtccca ggcgggaacg aagatggcga gccgccccgg gcgagccggc gccggaaggt
     1141 cggcggccga agggaggggc ctgccgaggc gcgcggcgcg gcgcgccagc cagagaagat
     1201 ccaccgccag atcgctgagc cccaggatca ggaacccggt ggcggcgaac agcgcggcct
     1261 cgcgcatgat gaagtcgacg gcgccgaaca acggtcccgc aatgtccatg gttctgcccc
     1321 cagagccgac ccgaccatca tctatttgcg cgggatgatt ggcaagtact tttcgaaagc
     1381 actgaattat tcggccccaa cggtcgcggt ggaccggctc gatacttgcg actgaattca
     1441 gtaatctgca cttgttcttg ccgggctccg gtccggcgga tcatgcgtcc ctccggagcg
     1501 ccgataaaat cccgaaaccg aatcccgtat acgttgtatc cccttctcag atataagggg
     1561 tccggcgctc ccgtccgcgg agcgcgggga attgtctggg gggacaaagc gcatgcgctg
     1621 gatcatcgcg gcggcattgg caggctggtt tttcctcgct cccgctcccg cgcgcgcctg
     1681 ggggcagggg gggcattata cggtctgcga gatcggctat ctgaacctgt cgccgagggc
     1741 gaaggcggag gtggacaggc tggtcgccct cgatggacgt ttcgcgagct tcaccgagac
     1801 ctgcaccttc cccgacaacc cggtctcgcg ggcgagcgag cattattcca actatcttcg
     1861 aagccagcgg cggatcgggc ccggctgccc gggcgggcgg ccctgcgtcc tcggcgcaat
     1921 cggcagcgat ctcgccatcc ttcgctccgg ctcggccacc gacgcccaaa aggcgatcgc
     1981 gatcctccat atcggccatt ggtatggcga cctccaccag ccgctccaca tctccttcgc
     2041 cgacgaccgc ggcggcaaca gcatcaaggc gccgggcccc tgctcgggca atttcgaggc
     2101 cagcctccat tcggtctggg acacctgcgt cgtcgagcgc cggatcttca gtcccggcac
     2161 cgaccggctg gcccgggcgc gggcggcggc ggcgatcctc aacggcacga tcaccggcgg
     2221 gcagcggcgg agctgggtgc gctccagccc ctggcaatgg gccggcgaat cctacaaggt
     2281 cacgatcgcc ggccggaccg gctattgcgt ccgaaggcgg ggcgcctgct ggtattcgga
     2341 ttccagtgcg gaattcgcca aggacgatcc gcagcgcacg ctgaccgtcg acgacgccta
     2401 tctcgaccgc gccaagccga tcgttgagac ccggctgcgg cgcgccggga tccggctcgc
     2461 cgacgccctc aaccggacct tcgatcccgg cttcgacggc tggcggtcgt gaagcttcgc
     2521 gccttgctca tcgccgccgc ggcgactctg gcgctcggcc ccgcctcgct cggcccgtgc
     2581 ctgatcggaa cggcggcggc gcagggcttt gcctgctgca aggtctgccg ggccggcaag
     2641 gcctgcggcg acagctgcat cgcccgcgac aagacctgcc acaagggcaa gggctgtgcc
     2701 tgcgatggct gagcggctgg cggcgatcgc cctggcactg gtggcgctcg ccggctgcga
     2761 ttcccgcgcc gccaccgcgc ccgccgcggc aacgggcgcc ggcagggccg aagcgtccgc
     2821 cgcgcccgaa gcgccggatt ctggaatcat cgccgcgggc gaacgcttcg cctgcacgcc
     2881 ggagaaggtc tgggacggcg acggtccgat ccactgcgcc gagggaccca aggttcgcct
     2941 cgcgggcatc gccgcgcgcg agatggacgg gagctgcagc cccggacatc cgtgccccga
     3001 tgcgagcgcc gaggcggcgc gggacgcttt ggcgggattg ctcgggcgga cgaccggaag
     3061 cgcctcgacg ggtcatctgc tcgtcgaggg gccggctctc caatgccttt ccgaaggctc
     3121 cgccaagggg agccgcaccg ccgcctggtg cacgtcgccg acggcgggcg atctcaactg
     3181 cgccatggtg gcgagcggga cggtcgcgaa atgggagcgt tactggcgcg gccatagctg
     3241 ccgataggcc gccgcgcctg ccacaacgcc cctccctttg cgcacgccca gcggctaagg
     3301 ccgccgcgat ggtcgaacct gtggcacgcc cgcgctccgc gctccgcgac ttcctcaaga
     3361 gcgaggcggc cggcggaatc gtgctgatgg cggccgcggc gctggccctg atcgtcgcca
     3421 actcgccttt cgccgaaacc tatcggcatc tcctgcacct cgagaccggg ccggtgctgt
     3481 cccccaggct cgggccgatg accctccatc tgtggatcaa cgacgggttg atggccgtgt
     3541 tcttcctgct cgtcggactg gagataaaac gcgagttcgt cgacggacgg ctgtcgacct
     3601 gggaccggcg gcgcctgccg gtgatcgccg cggccgtcgg aatggcggtt ccggccgcga
     3661 tctacctcgc cttcaccgcc ggagccgatg gcctcagccg aggctgggcg atcccggcgg
     3721 cgaccgatat cgccttcgcg atcggagtgc tggcgctgct cggcagccgg gccccgacct
     3781 cgctcaagct gttcctcacc accgtcgcca tcgccgacga tctaggcgcg gtcgcgatca
     3841 tcgccttggc ctataccgcc tcgctcgata ccctcgcgct cggcgcggcg gcggcgatcc
     3901 tcttcgtcat gtatgcgatg ggcaagggcg gcgtccggcc gctctggccc tatctgatcg
     3961 gcgccggcct gctttggtat gcggttttgc tgtcgggcgt tcatgccacc gtcgccggag
     4021 tgctggccgc cgccgccatc cccatcgtcg tcactccggg cgctccggat tcggcggcct
     4081 cgccgctcca ccggctggag catggcctca gcccctgggt ggccttcctg atcgtgcctt
     4141 tgttcggttt cgccaatgcc ggggtctcgc tggaaggggt cgggctggag cgcctgttcg
     4201 cgccgcttcc gcttggaatc gccgccggcc tgttcctggg caagcagctt ggaatcttcg
     4261 gcgccgtctg gctgaccgtc cgcttcggcc tcgcctcgcg gcttcgcggc gccacctggc
     4321 ttcaggtctg gggcgtcgcg atgctctgcg ggatcggctt caccatgagc ctgttcatcg
     4381 gcgccctggc ctttcccggc gatccggccc gggtcgagga ggcgaagctc gggatcctgg
     4441 ccggctcctt cgtctccgcg ctgggcggct atctggtgtt gcggcttgcc cccggccacc
     4501 gccgccatca ggaggaggag gcgcttcagg agcgcgagat cgaaagcgac ggggacgtgg
     4561 acgggttgga ggagcgcaag gcatgaagtg gctgtacctg ttcgcggcgg tcctgctcgc
     4621 cggctgcgtc tccgccgggc ccgcacgcga cagcctcgat tcgatcgctc gcgattatgt
     4681 gaagctccag ctcgccatcg gcgagaagga ggaaggctat atcgacgctt attacggccc
     4741 gcccgaatgg caggccgagg cgaagaccgg cgccgatacc ccggcggcgc tggcccagcg
     4801 cgccggagtc ctgaccgttc ggctgaagag ccttgccgac gaccgcctgc agccgatgga
     4861 gcggcggcgg cgcgacttcc tgctcgccca gctccgcgcc gcctccaccc ggctgcggat
     4921 gatgcagggc gagaagctga gcttcgccga cgaggcggag ggattgttcg gagtccggcc
     4981 ggagctgaag ccgctcgcgg cctacgaccc agtgctcgcg cgtctggaga ctctggtgcc
     5041 gggcaccggc accctggccg agcgggtcga ggcgttccag aatcgcttca ccatcccgaa
     5101 agagcggctg cagccggtgt tcgacgcggc gatcgccgaa tgcaggcgcc ggacgctggg
     5161 gcatgtcgcg atgcccgcca acgaggcgtt cactctggaa ttcgtcaccg gcaagagctg
     5221 gtcgggctac aattggtaca agggcaatgc gaccagcctg atccaggtca ataccgacct
     5281 gccgatccga atcggccgcg ccgtcgatct cggctgccac gaaggctatc ccggccacca
     5341 cgtctacaac gcgctgctcg agacgaagct cgcgaagggc cgcggctggg tcgagttcac
     5401 cgtctatccg ctctattcgc cgcagagctt catcgccgag ggcagcgcca attacggcat
     5461 cgacctcgcc tttcccggcg acgagagggt gcgttacgaa actcaggtcc tctatccgct
     5521 cgccggcctc tctcccgccg acgcgctcgc cttccaggcg ctccagcagt cgacgaagga
     5581 cctggccggc gcccgcttca ccatcgcccg cgagtatctg gaggggcgaa tggacaagga
     5641 acaggcgatc gccgccatcc ggaaatacca gcttgtgagc cgcgcccgag ccgagcagtc
     5701 gctggccttt accgagcagt atcgaagcta cgtgatcaat tacgggctcg gcgaggatat
     5761 ggtgcgcgcc tatgtcgagg ccgccgggcc gggccagccg gagcgctggg cggcgatgga
     5821 gaagatcctg tccgagccga cgctgccgaa ggagctggtg cgctgatgcg agtcttctgg
     5881 gacgagaggc agcggcttca cgccccggcc caggagcttc acaacggcgc cttcgcaccc
     5941 ttcgccgagc attgcgggcg ggtggactcg atgctcgccg cgcttggggc gacgcattcg
     6001 gtggcggatc gcggcgagga ccctctgctg cgcatccacc cgcggcccta tctcgacttc
     6061 ctcaaatccg ccttcgccga ctggaaggcc gccgggcggc cgggcgatgc gtcgggctat
     6121 gtctggccgg ttgtccgccg acgcccgctc gacctcgacc gcatcgacgc caggctcggc
     6181 cggtacagca tggatgcctc caccccgatc gccgagggga cctgggagag cgcctattgg
     6241 gcggcgcaga cggcgctgtc ggggctcgat gcggtgctgg ccggggagcg ctccgccttc
     6301 gcgctctgcc gcccgcccgg ccaccatgcc ggcgccgatt acatgggcgg ctattgctat
     6361 ctgaacaacg ccgccgtcgc cgccgaggcg gggcgggcgg cgggcatcgg ccggatcgcc
     6421 atcctcgacg tcgactatca tcacggcaac ggcacccagg acatcttcct cgagcgcggc
     6481 gacatattct tcgcctcgat ccacgccgat ccgaggaccg actatccctt ctactggggc
     6541 cattccgacg agcggggcgc gggcgagggg gagggcgcga ccctcaacct gccgcttccc
     6601 cgcggaaccg cgaggcgcga ctacgaagcc gcgctcgacc gcgccctgga ggtgatcgcc
     6661 ggcttcggcg ccggcctcct catctgctcc ttcggagccg acaccttcac cggcgacccg
     6721 atctgctcgt tcgctctgga aacggctgat tacgagccgg tggcgcggcg gatcgcctcg
     6781 ctagggctgc cgacgctgat cgtgatggag ggcggctacg cggtggacgc gctcggggcg
     6841 aacgtcgccg ggttcctgtc gggcttctag gcgccaccga acttgacttg aggcggagca
     6901 aaaaccccct ctcccctgcg gggagagggc agggtgaggg tttcggcttt cgaggatgct
     6961 cccgaagtca gagccccctc accccaaccc tctccccgca ggggagaggg ggctgaacac
     7021 cgacccgatt gaacggcgac ggcctcagtt cggagcgcct cccgcctcca gctccttgcg
     7081 ataggcgtcg accgaccagt cccggccgag gaacttcctc gccaggtcgt tcatcgaggc
     7141 cgatccgccg ggtgcgagaa cgaggtcgcg ataggctcgg gcggtggcgg ggtcgcgaag
     7201 ccccgccttg cggaagcggc tgagcaggtc cgcggccagc gcttccgacc attggtacgt
     7261 gtaataggaa gcgccgtagc cgctgagatg gccgaacgac gcctgcatgt ggctgccctc
     7321 gggattggga gcgagggcat agcggccgta ggtctcgttg aatcgccggg tgaggtcggc
     7381 gtcggccatg tcggcggaat aataatcaag ggcgacgctg gcgaggccga gctggctcat
     7441 cgtgccgaag gcgcgcccga aattgcggcc cgcgaccatc ttgtcgacca gcgtgcgcgg
     7501 gatcggccgg ccctgagcgt cggtggcgaa cttggccagc gtatcggcgt cccacaccca
     7561 ctcctcgagc agggtcgagg gcgcctcgat cacgtcattc tcgatttcgc cgaaattctg
     7621 catcgccagg ccgcgctggc cggcgaacag ccagtgcagg agatggccga actcgtggag
     7681 gaaggtctcg acctggccat gctccatcag cccctcgggg aagttggtga gcagggccgc
     7741 ggccggaagc acccggccct tgatcccgat ccggatcgga aacatcgcgg catgggtgta
     7801 tttgttgtcg cgcggatgca tgtcgaggta gaaacggccg atcgcctgac ccttctcgac
     7861 cagctcgtag gccctgacct ccggcgacca cacctcggtc gcccaggggc ggatctgcac
     7921 tccgaacaga tcctcggtga ggctgaatat gccggtgcgc accttgtcga agctgaaata
     7981 ggtgcggacc acggcgggat cgacgtcgaa cgtctccttg cggacctgcc gcaaggcata
     8041 gccggtgtcc caggagccaa ggccggcgag cttcgggtcg tccttcctga gccgggcgag
     8101 cagccgcgcc gcctccttct ccccgacggg cctcgccgcg gcggcgatct ggtcgacgaa
     8161 ggctttggtc cggaccgggt cgcgcgccat cctgttggcg agatcgtagg ccgcgaaatt
     8221 gggatagccg agaagcgagg cgagttcggc gcgctgcgcg aacagccgct tcaggaccgc
     8281 gtcgttggcg ggataggcac ggttcaacca ggcagtctgg acccttttgc gaaggggcgc
     8341 gctgtcggca tattgcaaga ccggcgcgat ctcggcgccc gtcatcctga tctcgaccag
     8401 accgtccggg ccgggcttat ggcggtcgag atagtcctgc ggcactccgg ccagctcttg
     8461 cggcttcgcc ttcaccaccg atttgtccga attgatgttg cggtcgaact cgatcccggt
     8521 ctcggtgatc tcgttctgga gcgcctcgat ccgggtcctc gtcgctccgt ccttggcgac
     8581 cccggcgcgg cggaaattgt cgagctggcg ctgcagcgtg tagcgcagct cgggcgccac
     8641 cccggcgcgc tcgaccgcct gcagccgctc atagacgggc cgcgacatct ggaatcgggt
     8701 caccatctgg ttggtccgct ggacgcaatc ctcggcggcg gcccggatcg cggcgtcggg
     8761 attggtgtcc ttgatcaaag gcggctcggc gaaggcggcg ctgacggcga gattgtagag
     8821 gtcgtcatag gccgcgatca gggtggccgg cggggtcgaa agcggcatcg cctcgacctt
     8881 cgactgaagg cttccgatcg ccgcgatccg gcggtcgcac atcgccttca cctccgaagc
     8941 cgatttcggc ctggccagga tgggaccgag cgcggcgtcg gaaaccggcg tgtagcctgc
     9001 ggccaaagcc ggcgcggccg cggtcagaag cagaatggcg gacagagact tcaaagcgtg
     9061 catgttattc cccggtagaa tcgtgccgga gagctaacat ggatgacgcg ccggcccaac
     9121 cggcgcgatc acagcttctc ggtcagctcc ggcaccagct tgaacaggtc gccgaccagg
     9181 ccgaaatcgg cgacctggaa gatcggggcg tcctcgtcct tgttgatggc gacgatcagc
     9241 ttgctgtcct tcattccggc gagatgctgg atggcgccgg aaatgcctac cgcgatatag
     9301 acttcgggag cgacgatctt gccggtctgg ccgacctgat agtcgttggg ggcatagccg
     9361 gcgtcgaccg cggcccgact cgcgccgacg gcggcgccga gcttgtcggc gagcggatcg
     9421 atgaaggcgt ggaactgctc ggacgagccg agcgcgcggc cgcccgagac gatgatcttg
     9481 gcgctggtca gctcgggtcg ctcgctggcg gcgatctcgg cgccgacgaa gctggacagg
     9541 acggactcct cgccggccgg caccgcctcg atctccccgc tgccgccctc ggccgccgcc
     9601 ttctcgaagg cggtgccgcg gacggtgatc accttcttcg catcggccga cttcacggtg
     9661 gcgatggcgt tgccggcata gatgggccgc tggaacgtgt cctcgccctc gatggcgatc
     9721 acgtcggaga tctgcatgac gtcgagcaaa gcggcaacgc gcggagcgac gttcttgccg
     9781 gtcgtcgtcg aggcggcgac gaaggcgtca tgaccctgca tcagccccgc cgcgagcggc
     9841 gcgacattct cggccagctg gtgggcgtaa gccgctccgt cggcgacatg gaccttgccg
     9901 gcgccggcga tcttcgacgc tgcttccgcg accccaccga cgccctcgcc ggcgacgagc
     9961 aggtgcacct cgcccagctt ggaggcggcg gtcacggcgg acagggtggc gtccttcaaa
    10021 tggccgtttt catgctcgac gagaaccagc gtcttcatca aatcactccc acggtccgga
    10081 ggcggtcgat cagctcgtcc accgattgca ccttgatacc ggcctcgcgc ttcgagggct
    10141 cgcggactcc gagcgtggcc agccgcggcg tggtgtcgac tccgtaatcc gccggggtct
    10201 tctg
//
LOCUS       DASWNU010000320         5178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_271648, whole genome shotgun sequence.
ACCESSION   DASWNU010000320 DASWNU010000000
VERSION     DASWNU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271648"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VGB57_06320"
     CDS             complement(<1..649)
                     /locus_tag="VGB57_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0131004.1"
                     /translation="MSGPEVITLGCRLNAAESEAMRLLAGDEEDLVIVNSCAVTNEAV
                     RQTRQAIRRAKKARPEARVVVTGCAAQVEPGTFAAMPEVDRVVGNWEKFDLSSFRRKP
                     EPRGVEGSAANLAEVPAFAGMTVEARDFNSSTERVRVSDIFAVRERAPHLLAGFTERS
                     RAFVEVQNGCDHRCTFCIIPYGRGDSRSVPAGLVVERVKALTAQGFREVVLTGVDV"
     gene            complement(646..1437)
                     /gene="dapF"
                     /locus_tag="VGB57_06325"
     CDS             complement(646..1437)
                     /gene="dapF"
                     /locus_tag="VGB57_06325"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0131005.1"
                     /translation="MKRRFRKMHGLGNDFVIFDGREAPVEMTEARVRALADRRTGIGC
                     DQLIVLEPSDSADVRMRIWNSDGGEVESCGNAARCVALLLGGAAWIETAGGLLAASGG
                     GSGATVDMGEPRFGWDEIPLAYPMDTAAMPVGWEDLREPFAVNVGNPHIVFFVEDSNA
                     VDLERLGPLIENDPLFPERVNVNIASVEDGALRLRVWERGSGLTRACGTGACATAVAA
                     VSRRLAGSPVEVRLPGGALNVAWGPGSTIAMSGPAALVFEGEVDL"
     gene            1542..3152
                     /locus_tag="VGB57_06330"
     CDS             1542..3152
                     /locus_tag="VGB57_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0131006.1"
                     /translation="MKHAAALLLAAAAALAMPASAAEDVSRTQINRLLDEGLNRSQVM
                     ETAHHLTDRIGGRLTNSPQMREAERWTRQRLREFGLANVRAEPFEFGRGWSIVSSSAR
                     MTAPRVKTLVAVPVAWTPPTDGTISAGIAVAPMREREDFEKYRGKLRGRIVLVSHPGT
                     GSEPMEPPFKRLSDEELRKLDTYQMPKHSPLQIEKSLREADFAEERDAFLKAEGALAW
                     VQMSRRDGGLLHGQGSGYRVGRTPALPAFEVSAEDYRRLARLALTDASPTLELASDVR
                     FHDEDFNAYNILADLPGTDSRAGYVMAGAHLDSWAAGDGAADNAAGSAVVMEAARILA
                     RLGVRPKRTIRFALWAGEEQGLLGSLAYVERHIGTRPPPEERYRHLRPGQTWRYRWPL
                     TLKPGHRDMIAYFNLDNGSGKIRGINTEGNVAAAPIFKEWLAPFAGLGAGTVAVRPTA
                     STDHVYFQLVGLPGYQFIQDPLDYSARLHHSSLDTYDHLKADDLRQASVIMAAFLLNA
                     ANRAEPLPRMPVPTQPRPTDPFEYPEED"
     gene            complement(3275..3607)
                     /locus_tag="VGB57_06335"
     CDS             complement(3275..3607)
                     /locus_tag="VGB57_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0131007.1"
                     /translation="MSVCHCLACKARTGSAFSWNSTWLEERVETEGEPRSWTRITEEG
                     GRWCTDHFCPERGSTAWYRIEARPGMITIPAGNFGDPEFGEPAYSWYAERRCPWVRVE
                     TEGPLEEG"
     gene            complement(4035..4496)
                     /locus_tag="VGB57_06340"
     CDS             complement(4035..4496)
                     /locus_tag="VGB57_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY0131008.1"
                     /translation="MSDPPLILVDADACPVKEEIYKVAFRHDAAVKIVSNAHLRVPAH
                     PLIERVVVGAGFDAADDWIAERAGPRTVVVTGDILLADRCLKAGAAVIGSNGKPFTSA
                     SIGAAVATRAIMADLRAGMDGPGGGPAPFAKADRSRFLSALDEALVRLKRG"
     gene            complement(4510..5148)
                     /locus_tag="VGB57_06345"
     CDS             complement(4510..5148)
                     /locus_tag="VGB57_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131009.1"
                     /translation="MKGLLSLLILLPLGTAAASAERSPAGTYRLVGEQDVASGLRLQP
                     NGRFQYFLSAGALDERAEGRWSGAGGAVTLTTEPKPVPPVFKPEPSARTGAPLSVKVN
                     SPGGGGIAGVDLRIGFEDGEPIEHYTQEDGWSLPDGEKRVPRWIELGVPMHAIASPRF
                     PIDLAAGDALAFTLVPNDLGTLDFTGVRVEVAKRALIVHRGPARLRYESVRE"
BASE COUNT          794 a         1934 c         1690 g          760 t
ORIGIN      
        1 tgacgtcgac cccggtcagc acgacctcgc gaaagccctg ggccgtgagc gccttcaccc
       61 gctcgacgac cagcccggcc ggaacgctcc ggctgtcccc gcgcccgtaa gggatgatgc
      121 agaaggtaca gcgatggtcg cacccgttct gcacctcgac gaaggcgcgg gagcgctcgg
      181 tgaagccggc gagcaggtgg ggggccctct cgcggaccgc gaagatgtcg gagacccgca
      241 ccctctccgt gctggagttg aagtcccgcg cctctaccgt catcccggcg aaagccggga
      301 cctcggcgag attagccgcg gagccttcaa ctccacgagg ttccggcttt cgccggaatg
      361 acgagaggtc gaacttctcc caattcccca ccacccggtc gacctcgggc atcgccgcga
      421 acgtccccgg ctcgacctgg gccgcgcagc ccgtcaccac cacccgggcc tccggccggg
      481 cctttttcgc cctccggatc gcctgccggg tctgccggac cgcctcgttg gtcacggcgc
      541 agctgttgac gatgacgaga tcctcctcgt cccccgccag aaggcgcatc gcctcgctct
      601 ccgcggcatt gagccggcag cccagggtga tcacttccgg gccgctcaca aatccacctc
      661 gccttcgaag accagggcgg cgggcccgct catcgcgatc gtgcttcccg gcccccaggc
      721 gacgttcagc gcgccccccg gcaaccggac ttcgacgggg ctcccggcca gccttcggct
      781 cacggccgcg acggccgtcg cgcaggcgcc ggtgccgcag gcccgggtaa ggccggaacc
      841 ccgttcccag actcgcagtc ggagggcgcc gtcctcgacc gaggcgatgt tgacgttgac
      901 ccgctccggg aagaggggat cgttctcgat cagcgggccg agccgctcga ggtcgaccgc
      961 attcgaatcc tcgacgaaga agacgatgtg cgggttgccg acattgacgg cgaacggctc
     1021 ccgcaaatcc tcccagccga ccggcatcgc cgccgtgtcc atcgggtaag cgagcgggat
     1081 ctcgtcccat ccgaagcggg gctcgcccat gtcgaccgtc gcccccgagc cgccccccga
     1141 ggccgcgagg agcccgccgg ccgtctcgat ccaggcggcg ccgccgagca ggagggcaac
     1201 gcagcgcgcg gcattgccgc acgattcgac ctcgccaccg tccgaattcc agatccgcat
     1261 cctgacgtcc gcggaatcgc tcggctccag aacgatcagc tggtcgcagc cgatgccggt
     1321 tcggcggtcc gccagagcgc gcacccgcgc ctccgtcatc tccaccggcg cctcgcgccc
     1381 gtcgaagatg acgaagtcgt tgcccagccc atgcatcttc cggaaccgcc gcttcatcgc
     1441 ccgcgcatct agggagggga ggggtccaag tccacctcac gagccgttgc cgcggcttcc
     1501 gggacgccat aaggcgcggc cgtcaccagc agggatcccc gatgaagcac gccgccgccc
     1561 tcctcttagc cgccgccgcc gccctcgcca tgcccgcttc cgcggccgag gacgtcagcc
     1621 gcacccagat caatcgcctc ctcgacgagg gtctcaaccg aagccaggtc atggagacgg
     1681 cgcatcacct gaccgaccgg atcggcgggc gcctcaccaa ctcgccgcag atgcgcgagg
     1741 cggagcgctg gacccgccag cgcctgcgcg agttcggtct cgccaacgtg cgcgccgagc
     1801 ctttcgagtt cggccggggc tggtcgatcg tctcgtcgag cgcccggatg acggcgccga
     1861 gggtgaagac gctcgtcgcc gtcccggtcg cctggacccc gcccaccgac ggcacgatca
     1921 gcgccggaat cgcggtggcg ccgatgcgcg agagagagga tttcgagaaa tatcgcggca
     1981 agctgcgcgg acgaatcgtc ctcgtcagcc atcccggaac cgggtccgag ccgatggagc
     2041 ccccgttcaa gaggctgagc gacgaggagc tgcgcaagct cgacacctac cagatgccga
     2101 aacactcgcc cctgcagatc gagaaatcgc ttcgcgaagc ggacttcgcc gaggagcggg
     2161 atgccttcct caaggccgaa ggcgcgctcg cctgggtgca gatgtcgcgc cgcgacggcg
     2221 ggctgcttca cggccagggc tccggctacc gggtcgggcg caccccggcg ctgccggcgt
     2281 tcgaggtttc ggcggaggat tatcgccgcc tcgcccggct cgccctgacc gacgcctcgc
     2341 cgaccctgga gctggccagc gacgtccgct tccacgacga ggatttcaac gcctacaaca
     2401 tcctggccga cctcccgggc accgattcca gggccggcta cgtcatggcc ggagcccatc
     2461 tcgacagctg ggcggcgggc gacggcgccg ccgacaacgc cgccggaagc gcggtggtga
     2521 tggaggcggc ccggatcctc gcccgcctcg gagtccggcc gaagcgcacc atccgcttcg
     2581 cgctctgggc cggagaggag cagggcctgc tcggctcgct cgcttatgtc gagcgccaca
     2641 tcggcacgag gccgccgccc gaggaacgct accggcacct gcgcccgggc cagacctggc
     2701 gctatcgctg gccgctgacc ctgaagccgg ggcaccgcga catgatcgcc tatttcaacc
     2761 tcgacaacgg ctcgggcaag atccggggca tcaatacgga agggaatgtc gccgcggcgc
     2821 caatcttcaa ggagtggctg gcgcccttcg cgggcctggg cgcgggcacc gtcgccgtcc
     2881 gcccgacggc aagcaccgac catgtctatt tccagctggt cggccttccc ggctaccagt
     2941 tcatccagga cccgctcgat tattccgcgc gcctgcacca cagcagcctc gacacttacg
     3001 atcacctcaa ggccgacgac cttcgccagg cctcggtgat catggccgcc ttcctcctca
     3061 acgccgccaa ccgcgccgag ccgctgccgc gaatgccggt gccgacacag ccgcggccga
     3121 ccgatccgtt cgaatatccc gaggaggatt aggatcttcg tcatcccggc gaaggccggg
     3181 atccatgaac acggtggcgc gagctggagc gggcgcggtg ttcatggatt ccggccttcg
     3241 ccggaatgac ggatcaacct gagtcgatca aggcctaccc ctcctcgagc ggtccctccg
     3301 tctcgaccct gacccagggg caacggcgct cggcatacca ggaataagcg ggctcgccga
     3361 actcgggatc gccgaaattg ccggccggga tggtgatcat gcccgggcgg gcctcgatcc
     3421 ggtaccaggc ggtggagccg cgctcggggc agaaatggtc ggtgcaccag cggccgcctt
     3481 cttcggtgat ccgggtccag cttcggggtt cgccttcggt ctcgacccgc tcctcgagcc
     3541 aggtcgagtt ccaggagaag gcgctgcccg ttcgcgcctt gcaggcgagg cagtggcaga
     3601 ccgagattcg ctccggctca ccccgaaccc ttgccttgag cgccgcagcg gcaactcgcc
     3661 gtccttgcct ccattaccct ctcccaattc gcctcgaacg aggctatcat agcggctcga
     3721 aaggagacgt catgcgcctt gctttcctcg ctctcgcctc ggtccttccg gcagcctcct
     3781 cggccctgcc ggccgcatcg gcgcccgccg cggccccggc tccgcaccgc ctgtccgaca
     3841 atgccatccc ctcgcgcctc tgccgggacg acatgaaggt ccgtccgatc cgctccccgg
     3901 acgccgccag gccgaggcct ctcggcgagc tgccggcggg cgacctcacc ctggccgtcc
     3961 tcaaccgggt cggcgagtgc atcgagccgg tgaccgtccg ccagggctac ggcggcttcg
     4021 ggcaacgctg aggctcagcc ccgcttcagc cgcaccagcg cctcgtccag cgccgagagg
     4081 aagcgcgacc ggtccgcctt ggcgaagggc gccggcccgc cgccggggcc gtccattccg
     4141 gcgcgaagat cggccatgat cgcccgggtc gccacagccg ctccgatcga ggccgaagtg
     4201 aacggcttgc cgttggatcc gatcaccgcg gcgcccgcct tgagacagcg gtcggcgagc
     4261 aatatgtcgc cggtgaccac cacggtccgg ggtccggcgc gctcggcgat ccaatcgtcg
     4321 gcggcgtcga agccggcgcc gaccacgacc cgctcgatca ggggatgcgc cggcacccgg
     4381 agatgggcgt tgctgacgat cttcaccgcc gcgtcgtggc ggaaggcgac cttgtagatc
     4441 tcctccttca ccgggcaggc gtcggcgtcg acgaggatga ggggcggatc gctcatcgcg
     4501 gcctcggggt cactcccgca ccgactcgta gcgcaaccgg gccgggccgc gatgaacgat
     4561 cagcgccctc ttggccacct cgaccctcac tccggtgaaa tcgagcgtgc cgagatcgtt
     4621 ggggacgagg gtgaaggcga gcgcatcgcc ggcggcgagg tcgatcggga agcgcggcga
     4681 ggcgatagcg tgcatcggca ctccgagctc gatccagcgc gggacccgtt tctcgccgtc
     4741 gggcaggctc cagccgtcct cctgcgtgta gtgctcgatc ggctcgccgt cctcgaaacc
     4801 gatgcgcaga tccactccgg cgatgccgcc gccgcccggc gagttcacct tcacgctgag
     4861 cggagccccg gtccttgccg aaggttccgg cttgaagacc ggcggcaccg gtttcggctc
     4921 cgtcgtgagg gtcaccgctc cgccggcgcc gctccagcgc ccctcggccc gctcgtcgag
     4981 tgccccggcg ctcaggaaat attggaaccg gccgttcggc tggagccgaa gcccggacgc
     5041 gacgtcctgc tcgccgacca gccggtaggt cccggcgggg ctgcgctcgg ccgaggcggc
     5101 cgcggtgccg agcgggagca ggataaggag gctgagcagg cctttcatcc cgccttattg
     5161 gcgcgctttg taccggcg
//
LOCUS       DASWNU010000321         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_116850, whole genome shotgun sequence.
ACCESSION   DASWNU010000321 DASWNU010000000
VERSION     DASWNU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116850"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..2259)
                     /locus_tag="VGB57_06350"
     CDS             complement(85..2259)
                     /locus_tag="VGB57_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0131010.1"
                     /translation="MTADHRRTPTPELWGGAECTIVRLGDGYRDQAAETGHKDRRSDI
                     DLMAELGLTTVRYPILWEAIAPESPDKLDFAWTDDRLAMLRERGIKVIGGLLHHGSGP
                     KYTELLDPDFPKKLADYAARVADRYPWIDMWTPVNEPLTTARFSALYGHWYPHTRNYG
                     AFLRALVNECKGTFEAMRAIRERIPGARLVQTEDLGKTFSTAPLRYQAIHENERRWLS
                     LDLLCGRVDERHPFFAFLRGAGVGYSALDRLASGEGKPDILGINHYLTSERFLDHRMH
                     LYPKHFHGSNGRDNYADAEAVRVKRLEDDTGFAPRLREAWDRYGIPIAITEVHHGCHR
                     DEQLRWFAEVWETATKLRQEGMDLRAVTIWSMFGNVDWRFLLTQRNGFYDTGAFDARS
                     GTPRPTVIAHAARAYAKGETFDHPALDSPGWWRRPPRLYPWSGSCKPPACGGRKLLIT
                     GATGTLGNAFARLCEERALPFSLTSRAEMDLCDPRSIARALERHEPWAVINTAGFVRV
                     ADAERESEACMAANAEGPAALAGACAAAGVPLVTFSSDLVFDGRLGRRYHEEDETCPT
                     TVYGRSKAEAERRVLAAGGQPLIVRTSAFFGPWDRYNFAWHVLSSLARGETVRACSRT
                     RVSPTFVPDLCHATLDLLIDGETGLWHLANDGELSWFDFARRVAEGGGYDPGLVVAED
                     SPEDTSTALWSKRGMMLRSFDSALEDFLGSVSLEEETAVAAE"
     gene            2423..2707
                     /locus_tag="VGB57_06355"
     CDS             2423..2707
                     /locus_tag="VGB57_06355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0131011.1"
                     /translation="MRVQHQELSFEDRRCEPRHEDLIDSAIIHFRGERHSVPVLNIST
                     RGTMIESALAPRLGETILVEFPECSRIHAFVRWARDGRIGLNFGHEMVLG"
     gene            complement(2875..3522)
                     /locus_tag="VGB57_06360"
     CDS             complement(2875..3522)
                     /locus_tag="VGB57_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0131012.1"
                     /translation="MALQFPYGALRRLVYALLLMTASAAPAEAKDVHILAFGDSLTAG
                     YGLPPGQGFAPQLEDMLRRNGVRAFVTNAGVSGNTAAQGRARLKWTLDGFKAKPDLAI
                     VALGANDMLRGLPPRQTRADLDAIVTELKRRDIPVLIAGMLAAPNLGSRYMAEYNRIF
                     PDLAREHGAELYPFFLAGVAGNPALALPDRVHPNFQGIKKMVSGITPTVLRALGR"
     gene            3541..>4201
                     /locus_tag="VGB57_06365"
     CDS             3541..>4201
                     /locus_tag="VGB57_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0131013.1"
                     /translation="MTGSYVIDASNVTLALGSGAGRVEILRGVALQVASGESVALLGP
                     SGSGKSSLMAVLSGLERATGGSIHIAGADFTRLGEDDLARARQGRIGIVLQAFHLLPT
                     MTALENVAVPLELAGREGAFELARAELEAVGLGHRLTHYPAQLSGGEQQRVAIARATA
                     PRPALLFADEPTGNLDAATGTAILDLLFERRKGTGATLLVITHDPALAARCGRVLEMQ
                     DG"
BASE COUNT          672 a         1405 c         1426 g          698 t
ORIGIN      
        1 cgcgcgatcc tctgaatcta cggcagttac ccctccacca ggcttcgcct ggtccccctc
       61 cccatctcgc gatggggagg caaatcactc cgccgctacg gccgtctctt cctcaaggct
      121 caccgagccg aggaaatcct cgagcgccga atcgaagctc cgaagcatca tgccgcgctt
      181 gctccagagc gccgtgctcg tgtcctccgg gctatcctcg gccacgacca gccccggatc
      241 gtagcccccg ccctccgcca cccgccgggc gaagtcgaac cagctcagct cgccgtcatt
      301 ggcgaggtgc cagagcccgg tctcgccatc gatgagcagg tcgagcgtcg cgtggcacag
      361 gtccggcacg aaggtcggcg agacgcgggt gcgcgagcag gcccggaccg tctcgccgcg
      421 ggcgaggctg gagagcacat gccaggcgaa attgtagcgg tcccacggcc cgaagaaggc
      481 gcttgtgcgc acgatcagcg gctggccgcc ggcggcgagc acgcgcctct ccgcctccgc
      541 cttacttcgt ccgtagaccg tcgtggggca ggtctcgtcc tcctcatggt agcggcggcc
      601 gaggcggccg tcgaagacga ggtcggacga gaaggtgacg agcggaacgc cggccgcagc
      661 gcaggcgccg gcaagcgccg ccgggccttc ggcattggcc gccatgcagg cctccgactc
      721 ccgctcggcg tcggcgaccc ggacgaagcc ggcggtgttg atcaccgccc agggctcgtg
      781 gcgctcgagc gccctggcga tcgagcgcgg gtcgcaaagg tccatctccg cccgactggt
      841 caggctgaag ggaagcgccc gctcctcgca gagccgggcg aaagcgttgc cgagagtgcc
      901 ggtggcgccg gtgatcagca atttacgtcc tccgcaggca gggggcttgc agctgccgct
      961 ccacggatag aggcgcggcg gccggcgcca ccaccccggg gaatcgagcg ccggatggtc
     1021 gaacgtctcg cctttggcat aggccctggc ggcgtgggcg atgacggtgg gccgaggcgt
     1081 cccgctccga gcatcgaaag cgccggtgtc gtaaaagccg ttgcgctggg tgagaaggaa
     1141 gcgccagtcg acgtttccga acatcgacca gatcgtgacg gcgcgcagat ccatgccttc
     1201 ctggcgaagc ttcgtcgcgg tctcccacac ttcggcgaac cagcgaagct gctcgtcccg
     1261 gtggcagccg tgatggacct cggtgatggc gatgggaatg ccgtagcggt cccaagcttc
     1321 cctgagccga ggagcgaagc cggtatcgtc ctccagccgc ttgacccgga ccgcttcggc
     1381 gtcggcataa ttgtcgcggc cgttacttcc gtggaaatgc ttcggataga ggtgcatccg
     1441 gtggtcgagg aagcgctcgc tggtcagata atggttgatt cccaggatgt cgggcttgcc
     1501 ctccccgctc gccagccggt cgagcgcgct gtagccgacc ccggcaccgc gaaggaaagc
     1561 gaagaagggg tgccgctcgt ccacccggcc gcagagcagg tcgaggctca gccagcgccg
     1621 ctcgttctca tggatggcct ggtagcggag cggcgccgtg gagaaggtct tgccgagatc
     1681 ctcggtctgg acgagccgcg cgcccgggat gcgctcgcgg atcgcgcgca tcgcctcgaa
     1741 cgtgcccttg cactcgttga ccagcgcccg aaggaacgcg ccataattgc gggtgtgggg
     1801 ataccagtgg ccgtacaaag ccgagaaacg cgccgtcgtc agcggctcgt tgaccggagt
     1861 ccacatgtcg atccagggat agcgatcggc caccctggcg gcataatcgg cgagcttctt
     1921 cgggaaatcg gggtcgagca gctcggtata tttcggaccg gagccgtggt ggaggaggcc
     1981 gccgatgacc ttgatcccgc gctcgcgaag catcgccagc cggtcgtcgg tccaggcgaa
     2041 gtcgagcttg tccgggcttt ccggagcgat cgcctcccac aggatcgggt agcgcacggt
     2101 ggtcagcccg agctcggcca tgaggtcgat atcgctccgg cgatccttgt gccccgtttc
     2161 cgctgcctga tctcgatatc cgtcaccgag ccgaacgatc gtgcattcgg cgccgcccca
     2221 tagttccggc gtgggcgtcc tgcgatgatc tgccgtcatt ccttctgcgt tccgattgtg
     2281 gcgggtgaga aacgaacccc ccccgtgaga ctagggttcc gcccgaagtc gccgcttcta
     2341 aagcaggtta gcgagattcc ggggcggtta ggaattcggt aacgcttttg ggtcatgctc
     2401 ccctgagacc ggggagagtg cgatgcgcgt ccagcaccag gagctgagct tcgaagaccg
     2461 ccgctgcgaa ccccgtcacg aagaccttat cgacagcgca atcatccatt tccgaggcga
     2521 gcgccactcg gtgccggtgc tcaacatatc gacccgcgga acgatgatcg aaagcgccct
     2581 cgcccctcgg ctgggtgaga cgatcctggt cgagttcccc gaatgcagcc ggatccacgc
     2641 cttcgtccgc tgggcccgcg acggccgcat cggattgaac ttcggccatg agatggtgct
     2701 gggctgattt gcctccccgt gcggagcatg gggaggggga ccaggcgaag cctggtggag
     2761 gggtaagggc acggatatct tgggatcgag cgcgaaccac ctgatccctg cgccatctac
     2821 ccctccacca cacttcgtgt ggtccccctc tccatctgcc gatggggagg caacttacct
     2881 ccccaaggcc ctcaataccg tcggcgtaat cccgctcacc atcttcttga tgccctggaa
     2941 gttgggatgc acccggtccg gcagcgccag cgcaggattg cccgccactc ccgccaggaa
     3001 gaaggggtag agctcggcgc cgtgctcgcg ggcgaggtcg gggaagatcc ggttatattc
     3061 cgccatgtag cggctgccga gattgggcgc ggcgagcatt ccggcgatca gaacggggat
     3121 gtcgcggcgc ttcagctcgg tcacgatcgc gtcgaggtcc gcccttgtct gcctcggcgg
     3181 caggcctcgg agcatgtcgt tggcgcccag tgccacgatc gcgaggtcgg gcttcgcctt
     3241 gaatccgtcc agggtccatt tcagcctcgc ccgcccctgc gccgccgtat tcccggaaac
     3301 gcccgcattg gtgacgaagg cgcgcacgcc gttgcgccgg agcatgtcct cgagctgcgg
     3361 agcgaagccc tggccgggcg gcaggccgta gcccgcggtg aggctgtcgc cgaaggcgag
     3421 gatgtggacg tccttcgcct cggcgggtgc ggcggaggca gtcatcagca gcagcgcgta
     3481 gacaagccgc cgaagcgccc catatgggaa ttgtaacgcc atcggattcc attccccttc
     3541 atgaccggct cctacgttat cgacgcttcg aatgtcacgc tcgcgctcgg ctccggcgcc
     3601 ggccgggtcg agattctgcg cggtgtcgcg ctacaagtcg cgagcggcga aagcgttgcg
     3661 ttgctcgggc cttcgggcag cggaaaatcg tcgctgatgg ccgtcctgtc ggggctggag
     3721 cgggcgacgg gaggctcgat ccatatcgcc ggcgccgatt tcactaggct gggcgaggac
     3781 gatctggcgc gggcccgcca gggacggatc gggatcgtcc tccaggcctt ccacctgctt
     3841 ccgacgatga cggcgctcga gaatgtcgcc gttccgctcg agctggccgg ccgggagggg
     3901 gcgttcgaac tcgcccgggc cgagctcgag gcggtcggac tcggccaccg cctcacccat
     3961 tatccggcgc aattgtcggg cggcgagcag cagcgcgtcg ccatcgcccg ggcgaccgcg
     4021 ccgcgcccgg ccctgctctt cgccgacgag ccgaccggca atctcgacgc cgcgaccgga
     4081 acggcgatcc tcgacctgct gttcgagcgc cggaagggga cgggggcgac gttgctagtc
     4141 atcacccacg atccggctct ggccgcgcgc tgcggacggg tgctggagat gcaggacggg
     4201 c
//
LOCUS       DASWNU010000322         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_271745, whole genome shotgun sequence.
ACCESSION   DASWNU010000322 DASWNU010000000
VERSION     DASWNU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271745"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VGB57_06370"
     CDS             <1..128
                     /locus_tag="VGB57_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEY0131014.1"
                     /translation="EPFLIQLGLVARTARGRCLNGRAWTHLGLPQPKTAQDGLFD"
     gene            147..1280
                     /locus_tag="VGB57_06375"
     CDS             147..1280
                     /locus_tag="VGB57_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEY0131015.1"
                     /translation="MTIIPTIAAAAALLTAAPAVAHDGPHYEQDSAWLCLPGRADPCG
                     APLATTALNANGYGSTGQVLPDKAAKVDCFYVYPTVSRDPGLNSDLEPGREEQATVAV
                     QLARFASVCRTFAPIYRQVTATALPRAFAGEDMRPAFATAYSDVLAAWKDFLARRNQG
                     RPFVLIGHSQGSIHLLQLIAEEVEGKPVAKKMLSAILLGWPVEVPEGRLVGGSLKSTP
                     LCSKAGQTGCVIAYMSFRASAPPPVGSFLGRAARPGMTAGCTNPAALRGGSAKLDSYW
                     FTMSPVPPGAQPVVWSSQGKPPTPFLRTEGLVTGECRHSGQAGYLAVTENADPNDART
                     DRIPGDVYIGGKLNPGWGLHLVDMNLAQGDLIRLVEAQAAKRR"
     gene            1540..1962
                     /locus_tag="VGB57_06380"
     CDS             1540..1962
                     /locus_tag="VGB57_06380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEY0131016.1"
                     /translation="MRGATHLFPLRVYFEDTDVAGIVYYANYLRFIERARSDMLRLIG
                     VDQRRALESGQGVYAVAEVAVKYRAPARLGDDLVVVTEIEEVRAASVLIHQRVMRGDR
                     LLADARVTAAFLSPEGRPRRQPGGWVEAFGRLSKEGKA"
     gene            1968..2648
                     /gene="tolQ"
                     /locus_tag="VGB57_06385"
     CDS             1968..2648
                     /gene="tolQ"
                     /locus_tag="VGB57_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY0131017.1"
                     /translation="MAMTGADMSPVALFLQADVVVKAVIVGLLLASIWTWAIIVGHAL
                     KLRRASTGSKDFERDFWKADDIDRFYEARGKADIPSAKVLAAGIAEWRRSTGGKVVDR
                     EGARSRLALVMHSALTEEVDRLAVRLNILATIGSVAPFVGLFGTVWGIMRSFTAIAGA
                     NNTSLAVVAPGIAEALFATALGLFAAIPAVIAYNRMSFGINRLEARLQRFADGFHGTL
                     SRELEMDA"
BASE COUNT          424 a          982 c          895 g          419 t
ORIGIN      
        1 tcgagccgtt cctcatccag ctcggcctcg tcgcccggac cgcccgcggc cgctgcctca
       61 acggcagggc ctggacccat ctcggcctgc cccagcccaa aacggcgcag gacggactgt
      121 tcgactgacc ttcaaggaaa cggaaaatga ccatcatccc caccatcgcc gccgcggcgg
      181 ccctgctgac cgccgcgccc gccgtcgccc atgacggccc gcattatgag caagactcgg
      241 cctggctctg cctgccgggc cgggccgacc cctgtggggc gcctctcgcg accaccgccc
      301 tcaacgccaa cggctacggc tcgaccggcc aggtgcttcc ggacaaggcg gcgaaggtcg
      361 actgcttcta cgtctatccg acagtctctc gcgatccggg cctgaacagc gaccttgagc
      421 cgggacggga ggagcaggcg accgtagcgg tgcagctggc ccggttcgcg agcgtctgcc
      481 ggaccttcgc gcccatctat cgccaggtga ccgcgacggc gcttccccgg gctttcgccg
      541 gcgaggacat gaggcctgct ttcgcgaccg cttattcgga cgtcctcgcc gcctggaagg
      601 atttcctcgc gcgtcgcaac cagggccgcc ccttcgtcct gataggccac agccagggct
      661 cgatccatct cctgcagctg atcgccgagg aggtcgaggg caagccggtc gcgaagaaga
      721 tgctctccgc catcctgctc ggctggccgg tggaggtgcc ggaaggcagg ctggtcggcg
      781 gcagcctcaa gtcgacgccg ctgtgcagca aggccggcca gaccggctgc gtcatcgcct
      841 acatgagctt ccgggcctcg gccccgccac ccgtcggttc cttcctcggc cgcgccgcca
      901 ggcccggaat gacggccggc tgcaccaacc cggcagcgct tcgcggcggc agcgcgaagc
      961 tcgattccta ctggttcacg atgtccccgg tcccgcccgg agcgcagccg gtcgtctggt
     1021 ccagccaggg caagccgccg acgcccttcc ttcgcaccga aggcctcgtc accggcgagt
     1081 gccggcacag cggtcaggcg ggctatctgg ccgtcaccga gaatgcggat ccgaacgacg
     1141 cccgaaccga ccggatcccc ggcgacgtct acatcggcgg caagctcaac cccggctggg
     1201 gcctccacct cgtcgacatg aacctcgccc agggcgacct catccgcctg gtggaagcgc
     1261 aggcggcgaa gcgccgataa tcctccccat cgttagatgg ggagggggac cacccgaagg
     1321 gtggtggagg ggtaacctcg caggcctcgt cagctacccc tccaccatgc gtcgcatggt
     1381 ccccctcccc atgctgcgca tggggaggat tcctcggccc ccggcgcaaa atcgtcacgc
     1441 ctcgtcccgc gcctcgcctt tggggttgcg gcggcgaatc ggcacccgat agagccgcat
     1501 cccatgggag gggacggacc gaagccggcg gacggaatca tgcggggggc gacgcacctc
     1561 ttcccgctgc gcgtctattt cgaggacacc gacgtcgccg gaatcgtcta ttacgccaat
     1621 tacctgcgct tcatcgaacg cgcccgctcc gacatgctcc ggctgatcgg ggtcgaccag
     1681 cgccgagccc tcgaaagcgg gcagggcgtc tacgcggtgg cggaggtcgc ggtgaagtat
     1741 cgcgccccgg ctaggctcgg cgacgacctc gtcgtggtca ccgagatcga ggaagtgcgg
     1801 gccgcgagcg tgctcattca tcagcgggtc atgcgcggcg accgacttct ggccgacgcc
     1861 agggtaaccg ctgccttcct cagtccggag ggccgccccc ggcgccagcc gggcggatgg
     1921 gtcgaggcgt tcgggcggtt gtcgaaggaa ggaaaagcgt gaccgatatg gcgatgacgg
     1981 gagccgatat gtcgcccgtc gctttgttcc tgcaggcgga cgtggtcgtg aaagcggtga
     2041 tcgtcggcct gctcctcgcc agcatctgga cctgggcgat catcgtcggc cacgcgctga
     2101 agctccgccg cgcctccacc ggctcgaagg acttcgagcg ggatttctgg aaggcggacg
     2161 atatcgaccg cttctacgag gcccgcggca aggccgacat ccccagcgcc aaggtgctcg
     2221 ccgccggaat cgccgagtgg cgccgctcga ccggaggcaa ggtcgtcgac cgggaaggcg
     2281 cccgctcgcg cctcgccctc gtcatgcatt cggcactgac cgaggaggtc gaccggctcg
     2341 cggtccgcct caacatcctc gccacgatcg gttcggtcgc ccccttcgtc ggcctgttcg
     2401 gaaccgtctg ggggatcatg cgaagcttca ccgccatcgc cggggccaac aataccagcc
     2461 tcgccgtcgt cgcgccgggg atcgccgagg ccctgttcgc gacggcgctc ggcctgttcg
     2521 ccgccatccc ggcggtgatc gcctacaatc gcatgagctt cggcatcaac cggctggagg
     2581 cgaggctgca gcgcttcgcc gacggcttcc atggcacgct gtcgcgcgag ctggagatgg
     2641 acgcttaaga tggccatggg acccatactc ggctccgggc ggcgcgggcg gcggacgcca
     2701 atggcggaga tcaacgtcac
//
LOCUS       DASWNU010000323         5782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_505537, whole genome shotgun sequence.
ACCESSION   DASWNU010000323 DASWNU010000000
VERSION     DASWNU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505537"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGB57_06390"
     CDS             complement(<1..684)
                     /locus_tag="VGB57_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY0131018.1"
                     /translation="MADFILVIDEGTTSTRAMLFSPDGSCLATEQRELAQSCPRPGWV
                     EHDAEEIWTSSLACARTVVAECGGAGRVAALGITNQRETIVFWSRRTGRALAPAIVWQ
                     DRRTADLCQRLKDEGHEEAVQSKTGLLLDPYFSASKIAWALEHWPRLGEAGDDLCAGT
                     VESWLVFRLTGGLHISDSTNASRTALMDIASGRWDDSLLDLWGVPAGILPEIVDCAGR
                     LGETLPEHFG"
     gene            complement(801..2030)
                     /locus_tag="VGB57_06395"
     CDS             complement(801..2030)
                     /locus_tag="VGB57_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY0131019.1"
                     /translation="MTYVDPAARARAYQSVAAPLRAGADSSSAAAYKLPVKGQGTIGF
                     ALSESAPFTVTARAGLLGSPTLRWTIGSYEVLLELVSIEGEITELLKEPGVGLDASLA
                     CPYWFSFDCHNRLLRYGKGEMRLGTLLASKELRRPKDGQPDPWTWIAAIEDVEVSAAT
                     TGFVDLWKDPVTVELPMRVVPHDSITMEDIAYGRVTVPASLTATCQKLYDNVAGARFI
                     LDTPDFPDFAAAIKASILDKDGWCAKVLEEKANEFGKYDPDMTYLRIALGTNQGESPG
                     IPFVMEIWPSGNYSPIHNHGGSDAVIRVLHGEINVSLYPFLAPEQQTPFAAKTFVRED
                     VTWISARLNQVHKLHNLNMEEPCITIQCYLYSDDNRTHWPYFDYLGEDDIKHFDPNSD
                     ADFLTFKRIMKEEWARR"
     gene            2124..3575
                     /gene="glpD"
                     /locus_tag="VGB57_06400"
     CDS             2124..3575
                     /gene="glpD"
                     /locus_tag="VGB57_06400"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY0131020.1"
                     /translation="MDSFDLLVVGGGINGAAIARDAAIRGASVLLVEKDDLAAHTSSA
                     SSKLIHGGLRYLEYYQFRLVREALAEREILLRTAPHIVRPLRFVLPHCPALRPRWMIR
                     AGLLLYDLLSVRGSLPRSRRVGRGEAALHSPLKERRRVLSYWDAWVDDSRLTVLNAVA
                     AAEHGAEIATRTEFLSARREGETWRAELSGGRRVAARAIVNAAGPWVADVLGRRLAET
                     SASRVRLVKGSHIVVPALWEGEQAYILQQADGRVVFALPFKGDFSLIGTTDIPVERPE
                     DSTISPEETRYLCEAADRYFTRQVVPDDVVWSYSGVRALRDDGSADAKSVTRDYRLEL
                     DPDPGPKLLSVFGGKITTARALAEEALDRLGIEGRRSTSHAFLPGGDLYPDFLAWLER
                     LAAWMPQPLLARLSGAYGTRLRDMIGDPDGLAHLGRHFGAGLYEAELRWLRDREFARS
                     ADDVLWRRTKLGLAMSATERDAVEAWMGGLLAE"
     gene            3742..4584
                     /locus_tag="VGB57_06405"
     CDS             3742..4584
                     /locus_tag="VGB57_06405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0131021.1"
                     /translation="MSNVDRATVRAFDDKWGASYRGVNPERDRQTFESYFSMFPFEEL
                     GEAEGFELGCGAGDQAARIAPKVGRLHCIDPTPNGLANARRIMAGQGNVEFHLAGVDE
                     IPLGDETQDFGYSMGVLHHIPDTRAALASCTAKLKAGAPFLLYLYYDFENRPLWFRAI
                     WKSSDYGRRLISRLPFRLRKMACDAIAATVYWPLARSAGLAARAGLPVEGLPLSSYRN
                     ARLVSMRVAALDRFGTGVEQRFSRAAIRAMMEDCGLTDIRIADGPPYWVALGRKRGGS
                     QGPS"
     gene            complement(4569..4694)
                     /locus_tag="VGB57_06410"
     CDS             complement(4569..4694)
                     /locus_tag="VGB57_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131022.1"
                     /translation="MGGDEQDLPSGDESIEEEAEESKRELDEVPEHGTDPLHEGP"
     gene            4765..5178
                     /locus_tag="VGB57_06415"
     CDS             4765..5178
                     /locus_tag="VGB57_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131023.1"
                     /translation="MTISAQQASALAGEGDSTIGWKVDREQRKELLQQFPPRYRNVDA
                     DHVTLRSRAADDARLPEESLGEIVGRADDGKGVEAMVVAIGGTVDRPDGSTYHITWSL
                     EPGRKAQESNDVLATREWTAFDLPMPVKLEPSRWP"
     gene            5121..5651
                     /locus_tag="VGB57_06420"
     CDS             5121..5651
                     /locus_tag="VGB57_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131024.1"
                     /translation="MDGLRPAHAGEARAVALALRVERQLYLDCDGVLADFDQGAAAVL
                     GMHPKAFEKRYGPGRFWAKLAKAPDFYFGLPLLDGATELFEAVKHLDPIILTGLPQGN
                     WAADQKVRWAAKHFPGTRIITTLARDKRKHAKAGDVLVDDQIRHAHLWEEIGGVFIHH
                     RSVADTLERLKEYFPL"
BASE COUNT          890 a         2013 c         1915 g          964 t
ORIGIN      
        1 tccgaaatgc tcgggcaggg tctcgcccag ccgtcccgcg cagtcgacga tctccgggag
       61 gatcccggcc gggacgcccc acagatcgag caggctgtcg tcccagcggc cgcttgcgat
      121 gtccatgagc gcggttcgcg aggcgttggt cgagtcggag atgtggaggc cgccggtcag
      181 gcggaagacg agccagctct cgaccgtgcc ggcgcacaga tcgtcccccg cctccccgag
      241 ccggggccaa tgctccagcg cccaggcgat cttggacgcg ctgaaatagg gatcgagcag
      301 aaggcccgtc ttcgactgaa ccgcctcctc atgcccctcg tccttcagcc tctggcagag
      361 atcggcggtg cgccggtcct gccagacgat cgcgggggca agggccctgc ccgtccggcg
      421 gctccagaag actatggtct cgcgctggtt ggtgatcccg agcgccgcca cccgccccgc
      481 gcccccgcat tcggcgacga cggtccgagc gcaggcgagg ctcgaggtcc agatctcttc
      541 cgcgtcatgc tcgacccagc cgggccgggg acaggattgc gcgagctccc gttgctcggt
      601 ggcgaggcac gatccgtcgg gcgagaacag catcgcgcgg gtgctggtcg tgccttcgtc
      661 gataacgagg atgaagtcgg ccatttgcct cccctgcccc agcgcacccg tcattgcgag
      721 gagcgaagcg acgaagcaat ccagcctgcc ttgggaggcc gcgctggatt gcttcgcttc
      781 gctcgcaatg acggggcccc tcaccgccgc gcccattcct ccttcatgat ccgcttgaag
      841 gtcaggaaat cggcgtcgct attggggtcg aaatgcttga tgtcgtcctc gccgagatag
      901 tcgaaatagg gccagtgggt gcggttgtcg tcggaataga ggtagcattg gatggtgatg
      961 cagggctcct ccatgttcag attgtggagc ttgtggacct ggttgagccg ggcggagatc
     1021 caggtgacgt cctccctgac gaaggtcttg gcggcgaagg gcgtctgctg ctcgggcgcg
     1081 aggaacgggt agaggctgac gttgatctcg ccgtgaagga cccggatcac cgcgtcggaa
     1141 ccgccgtgat tgtggatcgg cgagtaattc ccgctcggcc agatctccat gacgaacggt
     1201 atgcccggcg attcgccctg gttggtgccg agcgcgatcc tcaggtaggt catgtccgga
     1261 tcgtatttgc cgaattcgtt cgctttctcc tcgagcacct tggcgcacca gccgtccttg
     1321 tcgaggatgc tcgccttgat cgccgccgcg aagtccggga aatcgggcgt gtcgaggatg
     1381 aagcgggcgc ccgcgacatt gtcgtagagc ttctggcagg tggcggtgag gctcgccggc
     1441 acggtcaccc ggccgtaggc gatgtcctcc atggtgatgc tgtcgtgggg cacgacccgc
     1501 atcggaagct cgaccgtgac cggatccttc cacaggtcga cgaagcccgt cgtcgccgcg
     1561 gacacctcga catcctcgat ggcggctatc caggtccagg gatcgggctg cccgtccttc
     1621 ggccgcctga gctctttcga ggcgagcagg gtccccagcc gcatctcgcc cttgccgtag
     1681 cgtaacagcc ggttgtggca gtcgaagctg aaccaatagg ggcaggcgag cgaggcatcg
     1741 aggcccaccc ccggctcctt gaggagctcg gtaatctcgc cctcgatgga gaccagctcc
     1801 agcaacacct cgtaggaacc gatcgtccag cgcagggtcg gcgagcccag cagccccgcc
     1861 cgggccgtca ccgtgaacgg cgccgattcg gagagggcaa agccgatcgt cccctgccct
     1921 ttcaccggaa gcttgtacgc cgccgccgaa ctcgagtccg cgccggccct gagcggcgcc
     1981 gcgacgctct gataggcgcg cgcccgcgcc gccgggtcca cataggtcat gatgctctcc
     2041 cacgctcttt cgaggagcag gcacccccgc ccgcccagtc gatccttact gtatccgttt
     2101 cagcgccgcg ctaaaccccg cacgtggaca gtttcgacct cctcgtcgtc ggcggcggaa
     2161 tcaacggcgc ggcgatcgcc cgcgacgccg ccatccgcgg ggcgagcgtg ctcctcgtcg
     2221 agaaggacga tctcgccgct cataccagct cggcgtcctc caagctgatc cacggcgggc
     2281 ttcgctacct cgaatattac cagttccggc tggtccgcga ggccctcgcg gagcgcgaga
     2341 tattgctccg caccgcgccc cacatcgtcc gccccctgcg cttcgtcctg ccccattgcc
     2401 cggcgctgag gccgcgctgg atgatccgcg ccggtctcct cctctacgac cttctctcgg
     2461 tacgcggcag ccttccccgc tcccgccggg tcggccgcgg cgaggcggcg ctgcactcgc
     2521 ctttgaagga gcgccggcgg gtgctcagct attgggacgc ctgggtcgac gacagccggc
     2581 tcaccgtgct gaacgccgtc gccgccgccg agcatggcgc ggagatcgcg acccgcaccg
     2641 agttcctctc cgcccgccgc gagggcgaaa cgtggcgcgc cgagctgtcc ggcggacgcc
     2701 gggtcgcggc cagggcgatc gtcaacgccg ccggcccctg ggtcgccgat gtgctcggcc
     2761 gccggctcgc ggagaccagc gccagccggg tccggctggt caagggcagc cacatcgtcg
     2821 tcccggcttt gtgggagggc gagcaggcct atatcctcca gcaggccgac ggccgggtcg
     2881 tcttcgcgct tcccttcaag ggcgatttct cactcatcgg aaccaccgac attccggtcg
     2941 aacggcccga ggattcgacg atctcgcccg aggagacacg ctatctttgc gaggcggccg
     3001 accgctattt cactcgccag gtcgtcccgg acgacgtcgt ctggagctat tccggagtcc
     3061 gggcgctccg ggacgacggc tcggccgacg cgaaatccgt tacccgcgac tatcgactcg
     3121 agctcgatcc cgacccgggc ccgaaactcc tctccgtctt cggcggcaag atcaccaccg
     3181 cacgggcgct ggccgaggag gcgctcgacc ggctcgggat cgaaggccgt cgctccacct
     3241 cccacgcctt cctgcccgga ggcgacctct atcccgattt cctggcctgg ctcgagcggc
     3301 tcgcggcgtg gatgccccag cccctcctcg cccggctttc cggcgcctac gggacccggc
     3361 tcagggacat gatcggcgac ccggatggcc tcgcccatct cggccgccac ttcggcgccg
     3421 gcctctacga ggcggagctg cgctggctca gggacaggga gttcgcccga agcgccgacg
     3481 acgtgctgtg gcggcggacc aagctcgggc tggcgatgag cgccaccgaa cgggatgccg
     3541 tcgaagcctg gatgggcggg ctcctcgccg aatgacgccg gcggccccta gcccggacct
     3601 ccgcccgggt gtatccggag caagcgggca cctcgccata tcgccgccgc cggcggccgg
     3661 caggtgcgcc cggacaaggt gaccatgggg actgccagtg gttgaacgtt cgactgccct
     3721 tccggcgccg aggacgctgc gatgagcaac gtcgaccgag ccacggttcg cgctttcgac
     3781 gacaagtggg gcgcgtccta ccgcggggtc aatccggagc gcgaccggca aacgttcgaa
     3841 agctacttct cgatgtttcc cttcgaggag cttggcgagg cggagggatt cgaactcggc
     3901 tgcggcgccg gcgaccaggc ggcgcggatc gctccgaagg tggggcggct gcactgcatc
     3961 gatcccaccc cgaacggcct cgcgaacgcg cgaaggatca tggccggaca gggcaatgtc
     4021 gagttccacc tggccggggt ggacgagata ccgctcggtg acgagaccca ggacttcggc
     4081 tattccatgg gcgtcctcca tcacattccg gacacccggg ccgccctcgc gagctgcacg
     4141 gcgaagctga aggctggcgc gcccttcctc ctctacctct actacgattt cgaaaaccgg
     4201 cccctgtggt tccgggcgat atggaagtcg tcggactacg gccggcggct gatttccagg
     4261 cttccgttcc ggctccggaa aatggcctgc gacgcgatcg cggcgacggt ctattggccg
     4321 ctcgcgcgct ccgccggtct cgcggccagg gccgggctgc ccgtcgaggg cctgccgctg
     4381 agctcctaca ggaacgcccg tctggtcagc atgcgggtcg ccgcgctgga ccggttcggc
     4441 accggcgtcg agcagcgctt ctcgagggcc gcgatccgcg ccatgatgga ggattgcggg
     4501 ctgaccgaca tccgcatcgc cgacggcccg ccttactggg tggcgctggg ccgcaagcgc
     4561 ggcggttctc agggcccctc gtgaagcggg tccgtcccgt gctcgggcac ctcgtccagc
     4621 tcccgcttgc tctcctccgc ttcctcctcg atcgactcgt cgcccgaggg gaggtcctgc
     4681 tcgtcgccgc ccatgttggc tctccttcga gcccttgaac gccccagccg ggaacccgct
     4741 ccgccgtccg cgctttggca ggtcatgacg atttccgcac agcaagcctc cgctttggcg
     4801 ggcgaaggcg acagcacgat cggctggaag gtcgaccgcg agcagcgcaa ggagctgctc
     4861 cagcaattcc cgccccgcta ccgcaacgtc gacgcggacc acgtcactct tcgatccagg
     4921 gcggccgacg acgcgcgcct tccggaggag agcctcggcg agatcgtcgg ccgggccgac
     4981 gacggcaagg gcgtcgaggc gatggtcgtc gccatcggcg gaaccgtcga ccgtcccgac
     5041 ggaagcacct atcacatcac ctggtcgctc gagccgggac gaaaggcgca ggaatcgaac
     5101 gacgttctcg cgacgcgcga atggacggcc ttcgacctgc ccatgccggt gaagctcgag
     5161 ccgtcgcgct ggccctgagg gtggagcgcc agctctacct cgattgcgac ggagtgctcg
     5221 cggacttcga ccagggcgcg gcggcggtgc ttggaatgca ccccaaggcg ttcgagaagc
     5281 gctacggccc cggccgcttc tgggcgaagc tggccaaggc gccggatttc tatttcggcc
     5341 tccccctgct cgacggcgcc accgagctgt tcgaggcggt caagcatctc gatccgatca
     5401 tcctcaccgg ccttccgcag ggcaattggg cggcggacca gaaggtgcgc tgggcggcga
     5461 aacatttccc cggcacccgg atcatcacca ctttggcccg cgacaagcgc aagcacgcca
     5521 aagcgggcga cgtcctcgtc gacgaccaga tcaggcacgc ccatctctgg gaagagatcg
     5581 gcggcgtctt catccaccac cgcagcgtcg cggacacgct ggagcggctg aaggagtatt
     5641 ttcctctcta aatcctcccc gagcctgctc ggggaggggg accaggtgaa gcctggtgga
     5701 ggggcatccg gcgggagtat tcggtttctg agtattcggt gcctggcacc gaatgataag
     5761 tggttgattt tgttggcgag ga
//
LOCUS       DASWNU010000324         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_141474, whole genome shotgun sequence.
ACCESSION   DASWNU010000324 DASWNU010000000
VERSION     DASWNU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141474"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VGB57_06425"
     CDS             <1..748
                     /locus_tag="VGB57_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0131025.1"
                     /translation="GRSGTRPSPRRSICRSKRPEQVGGERAVRKLIGAAFVSLDGVMQ
                     APGAPEEDRTGGFALGGWLFNYFDEAVGTRIDTLFAGEFDLLLGRRTYDIFAAYWPFA
                     QGENKALGERFDRASKYVVTRGSQALPWRNSHRVADLDALAGVRSGEGPPLLIQGSGT
                     LYPQLLAAGLIDRLVLMTFPVILGSGKRLFGEGTPARSMLMVDRQVTPGGNIVATYEP
                     RGEVRPGSFATEEPSAAEMERRERIRRGVW"
     gene            799..1173
                     /locus_tag="VGB57_06430"
     CDS             799..1173
                     /locus_tag="VGB57_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0131026.1"
                     /translation="MIQHRAAAILPCNDIDATQAFYERLGFAMTAGEAGFRILADGKG
                     WQIALRPAEPGWVIPDHNSNGLYLYCDDVDAVADRVRDIIIEKGAPHRKPWGMYEFAV
                     GDPDGVLVRIGRPARECDPLHR"
     gene            1475..3058
                     /locus_tag="VGB57_06435"
     CDS             1475..3058
                     /locus_tag="VGB57_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY0131027.1"
                     /translation="MHPVETFELVLALLAATIALRWAAARVGFPPSAALLLGGGALAF
                     LPGIPEISLDPELALVLFLPPLLMDGAYYTAFGRFRRHLPGILSLAVGAVLFTTVVVA
                     VTIRWLVPTLPWAACFALGAIVSPPDAVAARAVLQRVRLPRRLQALLEGESLLNDATG
                     LVLFRFAVAATLSGTFDAGEAVGSFLLVAIGGAAVGIAVAFAWIAVMRRMTDETLVVA
                     TATLLCWAAYLGGEALHVSGVIATVAAGIVFGWHQHVVLAAKIRMQGSAFWRTLVFSL
                     EALVFILIGFSLRGVLERVGGAEAVLTTMAGPVLGIVAAVLLARFLWVFASEGALTLL
                     RRAGLARARPLGWRQAGVLGWAGMRGVVTLAVALALPEEMPGRDLMLVAAFAVILVTV
                     ILQGSSLGRLIGLLDPADEDPPAKIDMAGAEAAMARAKAASIEVHAYGPDGTLLHPQL
                     LEQYRHRAQAIQRYAGDADSFTPGIQAHFDAVLASVAAARAELVRLHRQGLIEDEVLH
                     DLERDLDLEELGALYQQGG"
     gene            complement(3112..3951)
                     /locus_tag="VGB57_06440"
     CDS             complement(3112..3951)
                     /locus_tag="VGB57_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131028.1"
                     /translation="MRSLIPPDRDTTADLDLCIAQAGPAGANVQAHRQHILDLFDRYI
                     ADRAWDFDRAQPLDHIPQAVKNALRASYSLTYPKRSLGGLRAELLSVPDNLCPYCRLE
                     SPATLDHFLAKSLHEPFAAFAPNLVPMCRVCNTLKGTKGSAIARQFFTHAYFDQLADG
                     ERFLVAQVAVGARHIATNFAIDFSATLDANVFQRLAYQFSILRLGSRYQLAAVDLIYE
                     QAMKLKNMELDGCGVEDRRRSLEGDARTEANGYGESYWKASLLAALAANVVFYTDGFN
                     RAL"
     gene            complement(3948..>4136)
                     /locus_tag="VGB57_06445"
     CDS             complement(3948..>4136)
                     /locus_tag="VGB57_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131029.1"
                     /translation="LTRESLGLPAERADYIGRLKEVIKKDTQLEDIEKLFGTRGMSSP
                     ARAQTLRIISAVQSGQKV"
BASE COUNT          610 a         1363 c         1430 g          733 t
ORIGIN      
        1 gggaagatcc ggcaccaggc cgtcgccgag gcgctcgatc tgccgttcga agcggcctga
       61 gcaagtcgga ggggagcgcg cggtgcgaaa gctgatcggc gccgccttcg tctcgctcga
      121 cggcgtgatg caggcgcccg gcgcgccgga ggaggaccgg acgggcggct tcgcgctcgg
      181 cggctggctg ttcaactatt tcgacgaggc cgtcggcacg cggatcgata cgctgttcgc
      241 gggcgagttc gacctcctgc tcgggcggcg cacctacgat atcttcgccg cctactggcc
      301 gttcgcgcag ggcgagaaca aggcgctggg ggagcgcttc gaccgggctt ccaaatatgt
      361 cgtgacccgc ggcagccagg cgctgccctg gcggaacagc caccgggtgg cggacctcga
      421 tgcgctcgcc ggggtgagga gcggcgaggg cccaccgctc ctcatccagg gcagcggcac
      481 gctctacccg caattgctcg cggccgggct gatcgaccgg ctggtgctga tgacctttcc
      541 agtcatcctc ggctccggca agcgtctgtt cggcgagggc acgccggcca ggtcgatgct
      601 tatggtcgac cgccaggtca cgccgggcgg caacatcgtc gcgacctacg agccccgggg
      661 cgaggtcagg cccggcagct tcgcgaccga ggagccgagc gcggcggaga tggagcggcg
      721 ggagaggata aggcgggggg tgtggtagag cggcgcaatt cgttccccat cgccgggcaa
      781 cgcggctagg ctggcgacat gatccagcac cgggccgccg ccatcctccc gtgcaacgac
      841 atcgacgcca cccaggcctt ttacgagcgc ctcggtttcg cgatgacggc cggcgaggcc
      901 ggctttcgga tcctcgcgga cggcaaaggc tggcagatcg ccctgcgtcc ggccgagcca
      961 ggctgggtga tccccgacca taattccaac ggcctctatc tctattgcga cgacgtcgac
     1021 gccgtcgccg accgggtgcg cgacatcatc atcgagaagg gcgcgccgca ccgcaagccg
     1081 tgggggatgt acgagttcgc cgtgggggat cctgacggag tgctggtcag gatcgggcgg
     1141 ccggcccgcg aatgcgatcc gctgcatcgg tgacggtcgc tgagcggagg agattaggct
     1201 gtatcgacat tcagcgagcc aggtctccaa atcctcccct gcatttgcag gggaggggac
     1261 catgcgaagc atggtggagg ggccttgctg actccgtaga acccctccat cgcgcttcgc
     1321 gcggtccccc tcccccggaa atccggggga gggcttggga gggaccgacc ttggctgaat
     1381 gtcgatacag cctggcgccc tattccgccc cgctcgcagg gggtgcatag acggcaggcg
     1441 cgccgcgggc atggcgccgg gcggggaggg gcccatgcat cctgtcgaga cgttcgagct
     1501 cgtgctcgcc ctgctggcgg cgaccatcgc gctgcgctgg gccgcagcca gggtcggttt
     1561 cccgccgtcg gcggccttgc tcctcggcgg cggggcgctc gccttcctgc ccggcatccc
     1621 cgaaatctcg ctcgatcccg agctcgccct cgtgctgttc cttccgccgc tgctgatgga
     1681 cggcgcctat tacaccgcct tcggccgctt ccgccggcac ctgccgggga tcctgtcgct
     1741 cgcggtcggt gcggtgctgt tcacgaccgt ggtcgtggcg gtgacaatcc ggtggctggt
     1801 cccgactctg ccctgggccg cctgcttcgc gctcggcgcg atcgtgtcgc cgcccgatgc
     1861 cgtcgcggcg cgagcggtgc tccagcgcgt gaggctgccg cggcggcttc aggcgctgct
     1921 cgagggcgaa agcctcctga acgacgccac cggcctggtc ctgttccggt tcgccgtcgc
     1981 ggcgactctc agcggcacgt tcgacgccgg cgaggccgtg ggcagcttcc tgctcgtcgc
     2041 gatcggagga gcggcggtcg ggatcgcggt cgccttcgcc tggatcgcgg tgatgcgccg
     2101 gatgaccgac gagaccttgg tggtcgccac cgcgaccctg ctttgctggg cggcctatct
     2161 gggcggcgag gcgctccacg tgtccggagt catcgccacc gtggcggccg ggatcgtctt
     2221 cggctggcac cagcatgtcg tcctggccgc gaagatccgg atgcagggca gcgccttctg
     2281 gcgcaccctc gtcttcagcc tcgaagccct ggtgttcatc ctgatcggct tctcgctgcg
     2341 cggcgtgctg gaacgggtcg gaggcgccga agccgtcctg acgaccatgg cgggaccggt
     2401 gcttggaatc gtcgccgccg tgcttctcgc ccgcttcctg tgggtgttcg cgagcgaagg
     2461 ggcgctgacc ttgctccggc gggccggact tgcacgcgct cgtccgctcg gctggcgcca
     2521 ggcaggcgtt ctcggctggg cgggaatgcg cggagtcgtg actttggcgg ttgctctcgc
     2581 cctgccggag gagatgccgg gccgagacct gatgctggtc gcggccttcg cggtgatcct
     2641 ggtcacggtg atcctgcagg gatcgagcct cggccgcctc atcgggctcc tcgacccggc
     2701 cgacgaggac ccgccggcga agatcgacat ggccggagcg gaggccgcca tggcccgcgc
     2761 caaggcggcg tcgatcgagg tccatgccta cgggcccgac ggcactctcc ttcaccccca
     2821 gctgctcgag cagtaccggc accgggcgca ggccatccag cgctacgccg gagacgccga
     2881 ttccttcacc cccggaatcc aggcgcattt cgacgccgtc ctcgcctcgg tcgccgccgc
     2941 ccgcgccgag ctggtccggc ttcaccgcca gggcctgatc gaggacgaag tgctccacga
     3001 cctggaacgc gatctcgacc tggaagagct tggggcgctg tatcagcagg gcggttgagg
     3061 gcgcgggcgg caggcgtcag atgtccggat cgaacgcaat tcgcctccgg gttacagcgc
     3121 gcggttaaac ccgtcggtgt aaaagacaac gttggcggcc aaggcggcca gcagggatgc
     3181 cttccagtaa ctctccccgt atccgttggc ttccgttctg gcgtctccct ccagcgagcg
     3241 ccgccggtcc tcaaccccgc atccgtccaa ttccatattc ttcagcttca tggcttgctc
     3301 gtatatcaaa tccactgcgg cgagctgata gcgggaaccg aggcgcagaa tgctgaattg
     3361 ataggcgagc cgctgaaata cgttggcgtc cagcgttgcg gagaaatcga tcgcgaaatt
     3421 cgtagcgata tggcgggctc cgactgcgac ctgggccacc aaaaatcgct cgccatcggc
     3481 cagttggtcg aaataagcat gcgtgaagaa ttgccgcgct atcgccgatc ccttcgtccc
     3541 cttcagagta ttgcagaccc tgcacatcgg gacgagattg ggggcgaaag cggcgaaggg
     3601 ctcgtgcaga ctcttcgcca ggaaatggtc gagcgtggcc ggcgactcga gtcggcaata
     3661 ggggcataga ttgtccggaa cgctcagcag ttcggccctg agtcccccca ggcttcgttt
     3721 cgggtaagtc aggctgtagc tggccctcag agcgttcttg acggcttgcg gaatgtgatc
     3781 gaggggctgc gccctgtcga agtcccaggc ccggtcggcg atgtatctgt cgaaaagatc
     3841 caggatgtgc tggcgatgcg cttgcacgtt ggccccggcg ggcccggctt gcgcgatgca
     3901 caggtcgaga tcggctgtgg tgtcgcggtc aggcggaatg agcgacctca aactttttga
     3961 cctgattgaa cagcgctgat gatgcgaagc gtctgagccc tggcggggct gctcatcccc
     4021 cgagtgccga agagcttctc gatatcctcg agttgggtgt cttttttgat tacctctttc
     4081 agtcggccga tataatcggc cctctccgcc ggcagaccga ggctctcccg agtcag
//
LOCUS       DASWNU010000325         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_246149, whole genome shotgun sequence.
ACCESSION   DASWNU010000325 DASWNU010000000
VERSION     DASWNU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246149"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VGB57_06450"
     CDS             <1..827
                     /locus_tag="VGB57_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY0131030.1"
                     /translation="QRARRRLLTAREEIATCAISGAVGTFANVDPRVEAHVAAKMGLG
                     IEPISTQVIPRDRHAMYFATLGVIASSVERLATEVRHLQRTEVLEAEEYFSPGQKGSS
                     AMPHKRNPVLTENLTGLARMVRGYVNPALENVALWHERDISHSSVERYIGPDATVTLD
                     FALARLTGVVDKLLVYPERMQRNLDLMGGLVHSQRVLLALTQAGASREQAYALVQRSA
                     MKVWKSDGALSLLELLKEDPEVSALLPPERLEESFDLGYHLKHVDTIFERVFGPSV"
     gene            879..1217
                     /locus_tag="VGB57_06455"
     CDS             879..1217
                     /locus_tag="VGB57_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0131031.1"
                     /translation="MDHQSRKAAVAAFKERKSQPGIYALTCRPTGERWVGRASDLTTI
                     WNRLSVALKMASTPHRSLQAAARAHGEAAFDFEVIERIDEADASAALVGAILKRRLEH
                     WREELEATAI"
     gene            1325..2023
                     /locus_tag="VGB57_06460"
     CDS             1325..2023
                     /locus_tag="VGB57_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0131032.1"
                     /translation="MTDLSQFPITRRWPAEHPERLQLYSLNTPNGVKVSIMLEETGLP
                     YESHMIDIGKNETWTEEFLSLNPNGKIPAIVDPDGPGGKPLGLFESGAILLYLADKTG
                     QLMPADPARRWETIQWVFFQMAAIGPMFGQVGFFNRFAGKAIEDKRPLQRYVDESKRL
                     LGVLEGRLEGRDWIMDEYSVADVATLGWVRNLVTFYEARELVGFDSLANVPAWLERGL
                     ARPAVQRGLEIPKR"
     gene            complement(2205..3242)
                     /locus_tag="VGB57_06465"
     CDS             complement(2205..3242)
                     /locus_tag="VGB57_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0131033.1"
                     /translation="MRVLILLLAFAAAACASSRNDPAITFANPVIDSDFPDPAILRAR
                     DGHYYVYATQTERDGKMINLQVARSRDLVAWEHLGDALPVKPGWASRTQDFWAPHVAE
                     HRGRYYLYYSAKPDSALTDDKKGLCLAVATADRPEGPFTDMGHPLECGPGFVNIDPMS
                     YDDPSTGARLLYWGSGFGPIKVRELAPDRLSFAPGSAPIDLIHADRKTDYRNLVEGAW
                     IVLRGGYYYLFYSGDNCCGKGVHYAVMVARSRSATGPFELQPGTLLERNAQWIGPGHN
                     SVIDDAYGESWIVYHAVDPKRTRTRPTDEVNTRRVMLIDHLVWTDEWPRVKGPTSGPQ
                     RRRYPERWAKD"
     gene            3404..>4462
                     /locus_tag="VGB57_06470"
     CDS             3404..>4462
                     /locus_tag="VGB57_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131034.1"
                     /translation="MVRSAHFALAALAGAALLASAGPALAQSEAWSRMAEEDLKAARA
                     FIEETHPAAVPDGRDPVFLGQLDKGYSAGQALAREARTFGGYRAAISRFAAAFDDPHI
                     ASASWVQPDNFWPGFLVSSRRGGWKVVPGGLADGPAAGAELVACDGKAPDALAAEWLG
                     PFTGSWTVAAERMRTSSSLLLDSGNPNQPRAQSCDFRSGDGKVAAHKLNWRRIGSADL
                     DRQFEKLGPIVSQDAGLRAFEGGWWIRLGTLSQAALPLMKAVEARQAEIRAAPFVVVD
                     LRGNGGGASFISDRIAEIVYGAARVDKAIYPKGAREPQKVVWRATPRTLGTADVYVER
                     AVRVGGADHPLALGMVAQR"
BASE COUNT          734 a         1447 c         1543 g          738 t
ORIGIN      
        1 tccagcgcgc ccgacgccgc ctgctgacgg cgcgcgagga gatcgccacc tgcgccatct
       61 cgggcgcggt cggcaccttc gccaatgtcg acccgagggt cgaggcccat gtcgcggcca
      121 agatgggcct cgggatcgag ccgatctcga cccaggtcat cccgcgcgac cgccacgcaa
      181 tgtatttcgc gaccctgggg gtgatcgcct cgtcggtcga gcggctggcg accgaggtcc
      241 gccacctgca gcgcaccgaa gtgctcgagg ccgaggaata tttctcgccc ggccagaagg
      301 gttcgtcggc catgccgcac aagcgcaatc cggtgctgac cgagaatctg accggactgg
      361 cgcggatggt gcggggctat gtcaacccgg cgctggagaa tgtggcgctg tggcacgagc
      421 gcgacatcag ccacagctcg gtcgagcgct atatcggtcc cgatgcgacg gtgaccctcg
      481 acttcgccct cgcccgcctc accggcgtcg tcgacaaatt gctcgtctat cccgagcgga
      541 tgcagaggaa tctcgacctg atgggcggcc tcgtccactc gcagcgggtc ctcctcgccc
      601 tcacccaggc cggtgccagc cgcgagcagg cctacgccct cgtccagagg agcgcgatga
      661 aggtgtggaa gagcgacggg gccttgtcgc tgctcgagct gctgaaggaa gatcccgaag
      721 tgtcggcgct gctgccgccg gagcgcctcg aggagagctt cgacctcggt tatcatctca
      781 agcatgtcga cacgatcttc gaacgggtgt tcgggccgtc ggtataatta tatccgggta
      841 atattgactc gggctttccg tgcccctatt ccgcatcgat ggaccatcaa tcacgcaagg
      901 cggccgtcgc ggcattcaag gagcggaaga gccagcccgg catctacgcc ctcacctgcc
      961 gtccgacggg cgagcggtgg gtcggccggg cctcggacct gacgaccatc tggaaccgcc
     1021 tgtccgtcgc cttgaaaatg gcgagcactc cgcatcgcag cctccaggcc gcggcgaggg
     1081 cccacggcga ggccgccttc gatttcgagg tgatcgagcg gatcgacgag gccgacgcct
     1141 cggcggcgct ggtcggagcg atcctgaagc ggcgactgga gcattggcgc gaagagcttg
     1201 aggcgaccgc gatctgattt accgtcattc cggacttgat ccggaatcca tgaacgccga
     1261 cgtgcgacac cgggttcggc cgtgttcatg gatgccggat caagtccggc atgacgaggc
     1321 agatatgaca gatctctccc aattcccgat cacccgccgc tggccggccg agcatcccga
     1381 gcggcttcaa ctctattcgc tgaacacgcc caacggcgtg aaggtctcga tcatgctcga
     1441 ggagaccggc ctcccctacg agtctcacat gatcgacatc ggcaagaacg agacctggac
     1501 ggaggaattc ctctcgctca acccgaacgg gaagatcccc gcgatcgtcg atcccgacgg
     1561 tcccggcggg aagccgctcg gactgttcga atcgggcgcg atcctcctct acctcgcgga
     1621 caagaccggg cagctgatgc cggccgatcc ggcgcggcgg tgggagacga tccagtgggt
     1681 cttcttccag atggcggcga tcggcccgat gttcggccag gtcggcttct tcaaccgctt
     1741 cgccggcaag gcgatcgagg acaagcgccc gctgcagcgc tacgtcgacg aatcgaagcg
     1801 gctgctcggg gtgctggagg gacggctcga ggggcgggac tggatcatgg acgagtatag
     1861 cgtcgccgac gtcgccaccc tgggctgggt gcgcaatctg gtcaccttct acgaagcgcg
     1921 cgagctggtc gggttcgact cgctggcgaa cgtcccggcg tggctcgagc gggggctggc
     1981 gaggccggcg gtgcagcgcg ggctggagat accgaagcgc tagcttgcct ccccatcgcc
     2041 agatggggag ggggaccacg tgtaacgtgg tgaaggggta actggcgccg cccgctgagg
     2101 gtcaagcgcc agacagcaaa ggccccgcgc cttctaccac tccaccaccc ttgggtggtt
     2161 cccctcccca tcttcgatgg ggaggaagat cagtgccgat gccttcaatc tttcgcccac
     2221 cgctccggat accggcgccg ctgcggcccg ctggtggggc ctttcacccg aggccattcg
     2281 tcagtccaga cgaggtggtc gatgagcatg acccgcctcg tgttgacctc gtccgtcggc
     2341 ctggtgcgcg tccgcttcgg gtcgaccgcg tggtagacga tccagctctc gccgtaagcg
     2401 tcgtcgatca cgctgttgtg gcccgggccg atccattggg cgttccgctc gaggagggtg
     2461 cccggctgaa gctcgaacgg gccggtggcg cttcgcgagc gggcgaccat cacggcgtaa
     2521 tgaacgcctt tgccgcagca attgtcgccc gaatagaaga ggtaataata gccgccgcga
     2581 aggacgatcc aggcgccttc gacgaggttg cgatagtccg ttttcctgtc ggcgtggatg
     2641 aggtcgatcg gcgcgcttcc cggggcgaag gacaggcggt cgggcgccag ctcccggacc
     2701 ttgatcgggc cgaagccgga gccccagtag agcagtcgcg ctccggtcga ggggtcgtcg
     2761 taggacatag ggtcgatgtt gacgaagccg gggccgcatt cgagcggatg gcccatgtcg
     2821 gtgaacgggc cctcgggccg gtcggcggtg gcgacggcga ggcacaggcc cttcttgtcg
     2881 tctgtaagag ccgagtcggg cttggccgaa taatagagat aatagcgccc gcgatgctcg
     2941 gccacgtgcg gcgcccagaa atcctgggtc ctgctcgccc agcccggctt caccggcaga
     3001 gcgtcgccga gatgctccca cgccacgaga tcgcgcgagc gggcgacctg gaggttgatc
     3061 atcttcccgt cgcgctcggt ctgggtcgcg taaacgtaat agtggccgtc cctggcgcga
     3121 aggatggcgg ggtcggggaa atcggagtcg atgacggggt tggcgaaggt gatggcgggg
     3181 tcgttccgcg acgaggcgca ggccgcggcc gcgaaggcga ggagcaggat caggacgcgc
     3241 atgcagccac atagcaactg tcgagccgcg aagcaccact cgcctcgccg cccacagtcc
     3301 aggggaatcg aaaatcctct cgagcgatgg ggcgttgtcg aagtgcctgg tccggattag
     3361 cctcggcgtg tgggcatcga cccgcgcacc gctggaggca tcgatggttc gttcggcaca
     3421 tttcgccctc gccgctctgg caggcgccgc cctcctcgcc tccgccggtc cggcgcttgc
     3481 ccagtccgaa gcctggagcc ggatggcgga ggaggatctg aaggccgccc gcgccttcat
     3541 cgaagaaacc caccccgccg cggtacccga cggccgcgat ccggtctttc tcgggcagct
     3601 cgacaagggc tattccgcgg ggcaggcgct ggcgcgggag gctcgaacct tcgggggcta
     3661 ccgagccgcg atcagccgtt tcgccgcggc gttcgacgat cctcacatcg cgagcgcaag
     3721 ctgggttcag cccgacaatt tctggcccgg tttccttgtc tcctcgcgtc gcggtggctg
     3781 gaaggtcgtg cccggcggcc tggccgacgg cccggcggcc ggagccgagc tggtcgcgtg
     3841 cgacggaaag gctccggacg ctctcgccgc ggaatggctc gggccgttca cgggatcgtg
     3901 gaccgtcgct gcggagcgaa tgcgaacctc ctcgtcgctg ctcctcgaca gcggcaaccc
     3961 gaatcagccg agggcgcaaa gctgcgactt ccgaagcggc gacggcaagg tcgcggctca
     4021 caagctgaac tggcgtcgga tcggcagtgc cgatcttgac aggcagttcg agaagctggg
     4081 tccgatcgtc tctcaggacg ccggcctgcg cgctttcgag ggcggctggt ggatccggct
     4141 cggaacgctc tcgcaggccg cgttgccgct gatgaaggcg gtagaggccc ggcaggcgga
     4201 aattcgggca gcgccgttcg tcgtcgtcga tctgaggggc aatggcgggg gggcttcctt
     4261 catcagcgat cgcatcgccg agatcgtcta cggggcggcg cgggtggata aggcgatcta
     4321 cccgaagggc gcccgggaac cgcagaaggt cgtctggcgc gccacccccc gaacgctcgg
     4381 gaccgccgac gtctatgtgg aacgggccgt ccgtgtcggg ggcgcggacc atccgctcgc
     4441 gctcggcatg gtggcccagc gc
//
LOCUS       DASWNU010000326        16465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_38565, whole genome shotgun sequence.
ACCESSION   DASWNU010000326 DASWNU010000000
VERSION     DASWNU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16465
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38565"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VGB57_06475"
     CDS             complement(<1..690)
                     /locus_tag="VGB57_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0131035.1"
                     /translation="MATREAVIVSTARTPIGKAYRGAFNATPSPTLASYAIKAAVERA
                     KIDPAEVDDVVMGAALQQGVQTTIGRTAALRSGLPVTVSGMSVDRQCASGLMAIATAA
                     KQIIVDRMPICVAGGVESISMVQTPEMRVGPDPELLSMHNGVYMPMIQTAEVVGSRYT
                     VSRERQDAYALRSQQLTARGQAEGKFDDEIVPVTARMMVKNKETGETSMQEVTLTKDE
                     GNRPQTTLEDLQ"
     gene            complement(708..1163)
                     /locus_tag="VGB57_06480"
     CDS             complement(708..1163)
                     /locus_tag="VGB57_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0131036.1"
                     /translation="MPIASIEDISAAVGREIGVSDWILVDQARIDAFAEITEDPQFIH
                     IDPEAAAKTPFGGTVAHGFLTLSLLSRMAADAMLRPEDVRMGVNYGFERVRFMAPVRS
                     GKRIRGRFTLVRLEEKKPGQWQFVHEVTVEIEGEEKPALVADWIGLIFI"
     gene            1218..4226
                     /gene="putA"
                     /locus_tag="VGB57_06485"
     CDS             1218..4226
                     /gene="putA"
                     /locus_tag="VGB57_06485"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY0131037.1"
                     /translation="MADATASARADLRELHRASEPEVLKPLLRDAALDTDSRRRVERR
                     ALAMLGELRAAQSSGWVNQFLQEYRLNTSEGIALLSLAEAFLRVPDPETADQLIADKL
                     GNADWRSHKGKSHSTLVNSATWGLVIGRALVSESDQASTLKRLLSRAGEPFVRQAVGA
                     AMRLMGEIFVMGRTIEEAIRRMEKRENAGFTASFDMLGEAARTFPDGERYFKSYEDAI
                     KAVGKVAKRGHSISVKLSALHPRYEVAQYERCVPSLIEQVEALALLAKGSDIAFTIDA
                     EESERLEMSLDIIEAVAGLPSLKGWDGLGMAVQAYGKRCRPTVAWADSIGASTGRRIA
                     VRLVKGAYWDSEIKRTQEQGLSDYPLFTRKASTDVSYLACARDMLAARNIYPAFATHN
                     ALTVATILEWAGASRDFEFQRLHGMGEGLYETLVREQGYHTRIYAPVGGHRDLLAYLV
                     RRLLENGANSSFVHQLADESLSDEDLIADPVAKIVAVGGTRHPAIPLPRDLFAPERPN
                     SQGLDLQDREELARVAKAVASAEEWKPAVTSTGEGSQRCKAEADSMVSRALAAFPAWD
                     ARPVDERAACIERLADLLERERDALMRICVQEARKTIPDALAEVREAVDFCRYYAAQA
                     RKGLQPVELPGPTGERNLLRMGGRGVWVCIAPWNFPLAIFLGQVSAALVAGNSVVAKP
                     APQTPEIAAYAVGLAHGAGIPEDVLVLAPGGPDMGAALVEDGRIAGIAFTGSTATAKR
                     IARELLNDDERPIIPFIAETGGINAMIVDSTALPEQVVVDVVTSAFRSAGQRCSALRV
                     LLLQEEIAERTLEMLSGAMDALVVGDPALPRTDVGPVIDQGAYERLMAHREETRGRWI
                     KTVEAPAQGLFVPPTLIRVDRVEDVKKEWFGPLLHVATWKAGRLAETVERVNRSGFGL
                     TMGLHSRIARAAETVEALATVGNLYVNRSMIGAIVGSQPFGGEGLSGTGPKAGGPHYL
                     PRFCAERVTSTDTTSAGGNATLLSLEDVGI"
     gene            4228..4473
                     /locus_tag="VGB57_06490"
     CDS             4228..4473
                     /locus_tag="VGB57_06490"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEY0131038.1"
                     /translation="MGRRRVHDFDSSAITRAAYDEDEATLDLWYKGGDRYRYFKVPAE
                     TFEALLSAPSAGEYVNRHIKDGFRFEIEARRKRFRPD"
     gene            4611..5627
                     /locus_tag="VGB57_06495"
     CDS             4611..5627
                     /locus_tag="VGB57_06495"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY0131039.1"
                     /translation="MTDLDTFIAGLPKAELHMHIEGSLEPELMFELARRNRVEIPFRS
                     VEEVRAAYSFSNLQDFLDIYYQGADVLRTEEDFRDLASVYFDRAAADSVTHSEIFFDP
                     QTHTARGIPFGVVMAGLLAGMAEANERHGLTSKLILCFLRHLDEEEALATLSEAEPWL
                     DRIEAVGLDSSELGHPPKKFARVFRAAAERGLKRVAHAGEEGPPDYVWQALDILEVDR
                     LDHGNRSLEDPLLVERLARERMTLTVCPLSNHKLRVVPDLAAHPIDRMLAAGLSATIN
                     SDDPAYFGGYVNDNYRAVAASLGRDALARLARNSFEGSFLGEEEKAAQMAKLDAYVSA
                     HPLP"
     gene            complement(5728..6873)
                     /locus_tag="VGB57_06500"
     CDS             complement(5728..6873)
                     /locus_tag="VGB57_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0131040.1"
                     /translation="MAGTTSNFVQKSSVLATEARAWVDGNIVGLLVAAAIGTAIVLAM
                     LAVRSLGCRMIARLGTDPHWPSIFARALAKTKIYFIVAASAQLVAGHASTPPALMEAI
                     HVLFVIAASLQAAIWARELILGYVAHRTGDQEQSTLASAIGIIRLLVTVTLFAIAIIV
                     ILDNLGVNVTGLIAGLGIGGIAIGLAAQGIFSDLFAALSILFDKPFRRGDTITFDQTT
                     GTVERIGLKSTRIRSINGEEVIVSNANLLNARLQNMTRLEHRRVLMLLNLVYRTPAEQ
                     LAALPDELKAIVEAQPGARFDRAHLTAFAASSIDLELVFFADAPDYAAFMNARQAVML
                     GILRRFAELEIEFAYPTQTSFTAGPDGRHIMPYPAVQRIVEADCGSE"
     gene            complement(6873..7904)
                     /locus_tag="VGB57_06505"
     CDS             complement(6873..7904)
                     /locus_tag="VGB57_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY0131041.1"
                     /translation="MLCFVDDRNLPGITRKRQGRYWQYFAPDGSRITDRDEIERLNAV
                     GMPPAYERCWFCPKPEGHILATGYDARGRKQYRYNPDFRMQQEAAKYERLAAFGRALP
                     KLRKKVEEDLTGKPTARETVLAAVVRLIDETRMRVGNEEYAKENKSFGATTLRNRHAR
                     VERGRLKISYTGKHGVKRTVTLTDRNLVRIARRTQDLPGQNLFEYVNDDGGVCPVTSG
                     DVNAYIKEAMGDAFTAKDFRTWGASTIAFEEMIRRYRKHGSVKLKSVIEPVAEALGNT
                     AAISRKSYVHPALIEAAKDAGAIGERPLPRASKYLSSAERGLIEFLDALPCEQARVDG
                     KGKVPAKAA"
     gene            7988..8536
                     /locus_tag="VGB57_06510"
     CDS             7988..8536
                     /locus_tag="VGB57_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY0131042.1"
                     /translation="MPNMSEAKILMIATHGYEDSELLEPRRILRERGADVTLASLEKG
                     EIEGDNGATARVDRTIGDVNAGDYDALVLPGGTKNPDKLRMDERVVATIRAFADAGKP
                     VAAICHGPWLLIEADLVRGRRITGWPSIRTDLRNAGGEVVDEEAVTDGNIVTSRKPED
                     IPAFTEALIALVEKVEAPLETA"
     gene            complement(8628..9743)
                     /locus_tag="VGB57_06515"
     CDS             complement(8628..9743)
                     /locus_tag="VGB57_06515"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0131043.1"
                     /translation="MSGAPADPRADLPALTGIRGLAAWFVVLYHIRVGAVAYLPERVS
                     FLLSKGYLAVDLFFMLSGFVLWLNYSDRLRRDGLAAAPKYLARRVARVWPLHLFILAA
                     TVVYASLVAATGTLNTVHYPWAELPLHVLLVHNWGFTSALTWNDPSWSISGEAAAYLL
                     FPLIVLAVDWRRLSPVWSIATLLLFAILLSAVMGWNGATILDRDIPRFGLLRAVTEFT
                     MGTIICALWRRWCSGPRAAAALSGALVAAALLLGFAAGAPETLVVPLFLAGLLLALAL
                     TANRPGNPLAARPIRYLGEISYSTYLVHFLLYIVFKIAFVEDPANVPPLLAGFFLLLT
                     FLASVALYHGVERPAQRALNRLFDRRLARRPLLSAAE"
     gene            complement(9740..11110)
                     /locus_tag="VGB57_06520"
     CDS             complement(9740..11110)
                     /locus_tag="VGB57_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HEY0131044.1"
                     /translation="MRDLFFVGFLLAVLALGLRRPFLFVLVYAYIDTVAPQRLSYYLL
                     NSLPLSMYVACFAFAGWLLFEKKAGLRFGPRQGLLAILLVYSFYTTSNAAFPIAAAEK
                     WDWASTSLAFAIFLPLTLRSKLRIEALLLFLTLSAAAIIIVGGLKTVASGGGYGVLNL
                     MVTNNSNLYESSTISTIAIALIPIILWLAKFGTVFPPDWRVKMFAAGLIFACLLMPIG
                     TEARTGLVCIAVLGLLTLRDVKRRFLYLAGAGLLVVASVPFLPDSFTGRMSTIQGYEG
                     DSSAKVRLAVWGWTWNYAQEHPLGGGFGAYLGNKIQVRTVTTQEQGGVQTIAARTEID
                     QGRAWHSAYFEMLGEQGFPGLILFLLIHIGGLVRMEVIRRRYRQSEGDKAWIAPLATA
                     LQHFQIIYLVGASFVAIAYTPFTLMILGVQIGLDNLIGRRERAERPRAAFAPAAASGQ
                     TEAAPA"
     gene            complement(11103..11267)
                     /locus_tag="VGB57_06525"
     CDS             complement(11103..11267)
                     /locus_tag="VGB57_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131045.1"
                     /translation="MIDNFSLALTHGLLLLVAWRLLSRTDLDEDPAPGTGPAASKIRR
                     WTLGRGKPDA"
     gene            complement(11493..12428)
                     /locus_tag="VGB57_06530"
     CDS             complement(11493..12428)
                     /locus_tag="VGB57_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0131046.1"
                     /translation="MATRVLLTVDTELRWDWHLSGASWRENLALSFDPAGVGVSYQLD
                     RLAAHGLKACFFVDPMPALLYGIEPVRLMVEPILAAGQEVQLHLHPSWADPANPVFEL
                     TGLDRRSQLDLIRTARDLLVEAGAPPPIAFRSGSYAADPATLEALAKLGIGYDSSHNG
                     SHHPWPSALPLDPRRIAPALLEGLVEIPITHIEQRPGSLRHLQICAVSFDEMRAALLH
                     AAGERHPVVNIVGHSFELATRGGKRPNPTLVRRFEKLCDFLAAHRERLPTAFFSDLAD
                     LRLDAEAFPLRSGAVRTAARMAQQAWANAVHERTL"
     gene            complement(12430..13356)
                     /locus_tag="VGB57_06535"
     CDS             complement(12430..13356)
                     /locus_tag="VGB57_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0131047.1"
                     /translation="MKPRFTLALAAAAAAIGPLQAQPSGPFTLAETGEAFGSLQEAVN
                     AIGRGSGTIRIAPGRYGECAVQEAGRVAYVAERRGEAVFDGGACEGKATLVLRGKGAR
                     VDGLVFTRVRVPDGNGAGIRLQEGDLDVSYTFFSDGQSGILSHDGPPGSISVDHSTFS
                     GLGEGDGVHSIYIGHYGSLRVTNSRFEQGRGGHYVKSRAPRIEVLDNSFDDSRGRRTN
                     YQIDLSNGAVGRIAGNVFVHGTGKENYATMISVAPEGVKNRSAGLVIEDNVASVAPGF
                     RWTTTFVGDWSGERLAIRGNRLGPKIETFGRR"
     gene            13421..14623
                     /locus_tag="VGB57_06540"
     CDS             13421..14623
                     /locus_tag="VGB57_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEY0131048.1"
                     /translation="MRILHVLDHSLPLHSGYTFRTRAIVKAQQAKGWEVACLTGARHS
                     APGSDPETLNGIDFYRTPHPPARPSPLREWQEIRALSARLDALVTAWKPDQLHVHSPV
                     LNALAALGVAKRRGVPLVYEIRAFWEDAAVGNGTGSEGSARYRLIRMLETRAARQADA
                     VAVICEGLRKDLVARGIEADKIIVAPNGVDMTLFGNPLAPDRDIARKLGLAGADVVGF
                     IGSFYDYEGLDDLIAAMPLLIAKRPKAQLLLVGGGPMEEALKAQAEASPAAERIHFIG
                     RVPHEEVDRYYSLIDVLAYPRKAMRLTELVTPLKPLEAMAQRKLVVASNVGGHRELIE
                     DGVTGSLFRAGDPEALADALDALFANRGKWEQRRDVARRFVERDRNWSSNISRYVPVY
                     QRLTGKAL"
     gene            14624..15424
                     /locus_tag="VGB57_06545"
     CDS             14624..15424
                     /locus_tag="VGB57_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131049.1"
                     /translation="MAKKVQKGGGSALDLAMAAFAGTAAGFVMFAMPDWQFDRAVELS
                     GLPLILPAAQPPLGMTARLAAIAAAAIGTWILVWLVLRALSQKPPEPKSRPKPVEIDV
                     PLPRLRRADAHPDAPARRPILAALELGAPLNAEPEAEQEGEETIVEAVKEEEAPEEEI
                     VLDSPLPSFIAEAEATEELEEADPDEDPEIPEIRDSVAPDGPAGEHEGASIAHLMQRL
                     ELGLLRRERTTWDGPTGEPRQNEAPSPQLDQRLRSAIDDLQRLAARGG"
     gene            complement(15425..15748)
                     /locus_tag="VGB57_06550"
     CDS             complement(15425..15748)
                     /locus_tag="VGB57_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131050.1"
                     /translation="MKRGNDATTAVIRAIIMNFMNFAVDFEEIRSRSWASVTFAGARH
                     ELTFRVSGEGAAEAADHFIRHLEAREFDLRGHIMADIALISEERRDGEDRVRISLEAL
                     TVEDA"
     gene            complement(15745..15933)
                     /locus_tag="VGB57_06555"
     CDS             complement(15745..15933)
                     /locus_tag="VGB57_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131051.1"
                     /translation="MHLLRRVEVYLRRSGMAPTRFGREAVRDPRFVFDLRKGREPRQR
                     IVNRVCAYLEPREGGSGR"
     gene            16033..>16465
                     /locus_tag="VGB57_06560"
     CDS             16033..>16465
                     /locus_tag="VGB57_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEY0131052.1"
                     /translation="MAEADPRAALQRLIEERREDYAGLSRLIGRNAAYIQQYVKRGTP
                     KRLAEEDRRLLARYFGVAETMLGAPEAPATRGDAPVVVPQFDLGASAGPGTLPEDERA
                     RSHLSFDAKWLRRLAGDPKQLSMIKVEGDSMLPTLSDGDEIL"
BASE COUNT         2765 a         5366 c         5749 g         2585 t
ORIGIN      
        1 ctggaggtcc tccagcgtcg tctgagggcg gttgccctcg tctttggtga gcgtgacctc
       61 ctgcatcgag gtctcgcccg tctccttgtt cttgaccatc atcctggcgg tgacgggcac
      121 gatctcgtcg tcgaacttcc cttccgcctg gcccctggcg gtgagctgct gggagcgcag
      181 agcataagcg tcctgccgct cgcggctgac ggtatagcgg ctgccgacca cctcggcggt
      241 ctggatcatc ggcatgtaga cgccgttgtg catggagagc agctccggat caggtccgac
      301 ccgcatctcc ggcgtctgca ccatcgagat gctctcgact ccgccggcga cgcagatcgg
      361 catccggtcg acgatgatct gcttggccgc cgtggcgatc gccatcaggc ccgaggcgca
      421 ctggcggtcg acggacatgc cggagacggt gacgggcagg cccgagcgca aggcggcggt
      481 tcggccgatc gtcgtctgca cgccctgctg gagcgccgcg cccatgacga cgtcgtcgac
      541 ttcggcggga tcgatcttcg cccgctccac cgccgccttg atcgcgtagc tggcgagcgt
      601 cggcgacgga gtcgcgttga aggcgccgcg ataggccttg ccgatcggcg tgcgggcggt
      661 ggaaacgata acggcttcgc gggtggccat gtctgggctc ctgttgatca gatgaagatg
      721 aggccgatcc agtcggcgac aagggcgggc ttctcctcgc cctcgatctc gacagtcact
      781 tcatggacga actgccactg gcccggcttc ttctcctcca agcggaccag ggtgaagcgg
      841 ccgcggatgc gcttgccgga gcgcaccggc gccatgaagc ggacccgctc aaagccgtaa
      901 ttgacgccca tcctgacgtc ttcgggcctc agcatcgcat cggcggccat tcggctgagc
      961 agcgacaagg tgagaaagcc gtgggcgacg gtgcctccga acggggtctt cgccgcggct
     1021 tccggatcga tgtggatgaa ctgcgggtct tcggtgatct cggcaaaggc gtcgatgcgt
     1081 gcctggtcca ccaatatcca gtcggagact ccgatctcgc ggccgaccgc ggcgcttatg
     1141 tcttcgatgc tggcgatggg catggaggcc gtctagagcg cccgggacac gagactcaag
     1201 ccctgcgagg aaacgacatg gcggacgcta cggcaagcgc gcgcgccgat cttagggagc
     1261 tgcaccgggc gagcgaaccg gaggttctga agccgctgct gcgcgacgcg gcactcgata
     1321 ccgattcgcg gcggcgggtc gaaagaaggg cgctggcgat gctcggcgag cttcgagccg
     1381 cccagtcgtc gggctgggtc aaccagttcc tccaggaata tcggctgaac acctcggaag
     1441 gcatagccct cctctcgctc gccgaggcgt tcctccgggt tccggacccg gaaacggccg
     1501 accagctgat cgcggacaag ctcggcaatg ccgactggcg ctcgcacaag ggcaagtcgc
     1561 attcgacgct ggtcaattcc gcgacctggg gcctcgtcat cggccgggcc ctggtcagcg
     1621 agtccgacca ggccagcacg ttgaagcgcc tcctctcccg cgccggcgag ccgttcgtcc
     1681 gccaggcggt gggcgcggcg atgcggctga tgggcgagat cttcgtcatg ggccggacca
     1741 tcgaggaggc gatccggcgg atggagaagc gcgagaatgc cggcttcacc gccagcttcg
     1801 acatgctcgg cgaggccgcg cgcaccttcc ccgatggcga gcgctatttc aaatcctatg
     1861 aggacgcgat caaggccgtc ggcaaggtcg ccaagcgcgg gcacagtatc tcggtcaagc
     1921 tctccgcgct ccacccgcgc tacgaggtgg cccagtacga gcgctgcgtt ccctcgctga
     1981 tcgagcaggt cgaggcgctg gcgctgctgg cgaaaggatc ggacatcgcc ttcaccatcg
     2041 acgccgagga gagcgagcgg ctcgagatga gcctcgacat catcgaggcc gtggcgggcc
     2101 tgccttcgct caagggctgg gacgggctcg gcatggccgt ccaggcctat ggcaagagat
     2161 gccgcccgac cgtcgcctgg gctgattcga tcggcgcctc gaccgggcgc cggatcgcgg
     2221 tccggctggt caagggcgcc tattgggata gcgagatcaa gcgaacccag gagcagggcc
     2281 tgtccgacta tcccctgttc acccgcaagg cctccaccga cgtctcctac ctcgcctgcg
     2341 cccgggacat gctggcggcc aggaacatct accccgcctt cgccacccac aatgccctca
     2401 ccgtcgcgac gatcctcgaa tgggcgggcg cgagccgcga cttcgagttc cagcggctgc
     2461 acggaatggg cgaggggctg tacgagaccc tggtccgcga gcagggctac cacacccgta
     2521 tctacgcgcc ggtggggggt catcgcgacc tgctcgccta cctggtccgc cgactgctgg
     2581 agaacggcgc caattcgagc ttcgtccacc agctcgccga cgaaagcctc agcgacgagg
     2641 atctgatcgc cgatccggtg gcgaagattg tcgccgtcgg cggcacccgc catcccgcca
     2701 taccgctgcc gcgggacctg ttcgcaccgg agcggccgaa cagccagggc ctcgacctcc
     2761 aggaccgcga ggagcttgcc cgggtggcga aagccgtggc gtcggccgag gaatggaagc
     2821 cggcggtgac ctccacgggc gaaggctcgc aacgctgcaa ggccgaagcg gactccatgg
     2881 tctcccgcgc cctcgccgcc tttcccgcct gggacgcccg cccggtcgac gagcgcgccg
     2941 cctgcatcga gcggctcgcc gacctgctcg agcgggagcg cgacgcgctg atgcggatct
     3001 gcgtccagga agcgcgcaag acgatcccgg acgccctcgc cgaggttcgc gaggccgtcg
     3061 acttctgccg ctattatgcc gcccaggcgc gcaagggcct gcagccggtc gagctgccgg
     3121 ggccgaccgg tgagcgcaat ttgcttcgaa tgggcggccg cggggtctgg gtgtgcatcg
     3181 cgccctggaa cttcccgctc gccatcttcc tcggccaggt cagcgcagcc cttgtcgccg
     3241 gcaacagcgt ggtcgccaag cccgccccgc agacgcccga gatcgccgcc tacgcggtcg
     3301 gcctcgccca cggcgccgga ataccggagg acgttctggt gcttgcgccg gggggaccgg
     3361 acatgggcgc cgccctggtc gaggacgggc ggatcgccgg aatcgccttc accggctcga
     3421 ccgcgaccgc caagcgcatc gcccgcgagc ttctcaacga cgacgagcga ccgatcatcc
     3481 ccttcatcgc cgagacgggc gggatcaacg cgatgatcgt cgattcgacc gctttgcccg
     3541 agcaggtcgt ggtcgacgtg gtgacgagcg ccttccgttc ggccgggcag cgctgctcgg
     3601 cgcttcgagt gcttctcctc caggaggaga ttgcggagcg gactctggag atgctgtccg
     3661 gagcgatgga cgcgctggtc gtcggggatc ccgccttgcc gaggaccgac gtcggcccgg
     3721 tcatcgacca gggcgcttac gagcggctga tggcgcatcg cgaggagacc cggggccgct
     3781 ggatcaagac ggtggaggcg cccgcgcagg gcctgttcgt gccgccgacc ctgatccggg
     3841 tcgatcgggt cgaggacgtg aagaaggaat ggttcggccc gcttctccac gtcgccacct
     3901 ggaaggccgg ccggctcgcg gagacggtgg agcgggtcaa ccgctccggc ttcgggctga
     3961 ccatgggcct gcacagccgg atcgcgcgcg ccgccgagac ggtcgaggcg ctggcgacgg
     4021 tcggcaacct ctatgtcaac cgctcgatga tcggcgccat cgtcggctcg cagcccttcg
     4081 gcggcgaggg cctgtccggc accggcccga aggcgggagg gccgcattac ttgccgcgct
     4141 tctgcgccga acgggtgacc agcaccgaca cgaccagcgc cggcggcaac gccaccttgc
     4201 tgtcgctgga ggatgtgggg atctgagatg gggcggcggc gggtccacga cttcgattcc
     4261 agcgccatca cccgagccgc ttatgacgag gacgaagcca cgctcgacct ctggtacaag
     4321 ggcggcgacc gctaccgcta tttcaaggtg cccgccgaga cgttcgaggc cctgctctcc
     4381 gcgccttcgg cgggtgaata tgtcaaccgg cacatcaagg acgggtttcg cttcgagata
     4441 gaggcgcggc ggaaacggtt ccggccggat tgatccgctc tggcagagcc ctccccgtca
     4501 tgctgaactt gtttcagcat ccagttctcc accccccggg cgggtgaccg gctggaccct
     4561 gaaacgagtt cagggtgacg gaggagggtg tggcggctac cctccctccc atgaccgacc
     4621 tcgacacctt cattgccggc ctgcccaagg ccgagctgca catgcatatc gagggcagcc
     4681 tcgagcccga attgatgttc gagctcgccc ggcgcaaccg ggtggagatt cctttccgaa
     4741 gcgtcgagga ggtgcgggcg gcctacagct tctcgaacct gcaggacttc ctcgacatct
     4801 attaccaggg cgcggacgtg cttcggacgg aggaggattt ccgcgacctc gcctcggtct
     4861 atttcgaccg cgccgcggcc gactccgtga ctcactcgga gatcttcttc gatccgcaaa
     4921 cccataccgc ccgcggcatc cccttcggcg tggtgatggc gggactgctg gccggcatgg
     4981 cggaggcgaa cgagcggcac ggcctgacct cgaagctcat cctctgcttc cttcgccacc
     5041 tcgacgagga ggaagccctc gccaccttga gcgaggccga gccgtggctg gaccggatcg
     5101 aggcggttgg gctggattcc tccgagctcg gccacccgcc gaagaagttc gcccgggtct
     5161 tccgggccgc ggcggaacgg ggcctgaagc gagtcgccca tgcgggggag gaggggccgc
     5221 cggattatgt ctggcaggcg ctcgacatcc tcgaagtgga tcgcctcgac catggcaacc
     5281 ggtcgctcga ggacccgctt ctggtcgagc ggctggctcg ggaacggatg acgctcaccg
     5341 tctgcccgct ctcgaaccat aagctccgcg tggttcccga tctggcggcg cacccgatcg
     5401 accgcatgct tgcggcgggc ctcagcgcca ccatcaactc ggacgatcca gcctatttcg
     5461 gcggctacgt gaacgacaat taccgcgcag tcgccgcctc gctcggccgc gacgcgctgg
     5521 cgaggctggc gcgcaacagc ttcgagggat cgttcctggg ggaggaggaa aaggcggcgc
     5581 agatggcgaa gctggacgcc tatgtgagcg cccatccgtt gccttgaacc gtcattgcga
     5641 gcgtagcgaa gcaatccagt gcggcttccg gcgcccctct ggattgcttc gtcgcttcgc
     5701 tcctcgcaat gacggagggt gggcgcccta ttccgacccg caatccgctt cgacgatcct
     5761 ctggacggcc ggatagggca tgatgtgccg cccgtccggc ccggccgtga aagaggtctg
     5821 ggtcggatag gcgaattcga tttcgagctc ggcgaagcgg cgcaatatgc cgagcatcac
     5881 cgcctggcgg gcattcatga aagcggcata atccggagcg tccgcgaaga agaccagttc
     5941 gaggtcgatc gaggaggcgg cgaaggcggt gaggtgggcg cggtcgaagc gggcgccggg
     6001 ctgggcctcg acgatcgcct tcaactcgtc gggcaaagcg gcgagctgct cggccggggt
     6061 gcggtagacg aggttcagga gcatcagcac ccgccggtgc tcgagcctcg tcatgttctg
     6121 gagccgggca ttgagcagat tggcgttgga gacgatcacc tcctcgccgt tgatcgaacg
     6181 gatccgggtg ctcttgaggc cgatccgctc gaccgtgccg gtcgtctggt cgaaggtgat
     6241 cgtgtcgccg cggcggaagg gcttgtcgaa caggatcgaa agcgccgcga agagatcgga
     6301 gaagatgccc tgggcggcga ggccgatggc gatgccgccg atgccgaggc cggcgatcag
     6361 gccggtgacg ttgactccca gattgtcgag gatgacgatg atcgcgatgg cgaagagggt
     6421 caccgtcacc agcagccgga tgatcccgat cgccgaggcg agcgtgctct gctcctggtc
     6481 gccggtgcgg tgggcgacgt agccgaggat cagctcgcgc gcccagatcg cggcctgaag
     6541 cgacgcggcg attacgaaga ggacatggat ggcctccatc aaggccgggg gcgtgctcgc
     6601 atgtccggcg acgagctgcg ccgaggccgc gacgatgaaa tagatcttgg tcttggcgag
     6661 cgcccgggcg aagatgctcg gccagtgcgg atcggtgccg agccgggcga tcatcctgca
     6721 gccgagcgag cggaccgcca gcatcgccag gacgatcgcg gtgccgatcg ccgccgcgac
     6781 gagcaggccc acgatattgc cgtcgaccca ggcccgggcc tcggtcgcga gaacgctgct
     6841 cttctggacg aagttcgacg tcgtgcctgc catcaggccg cctttgcagg aactttaccc
     6901 ttcccgtcca ccctcgcctg ctcacagggc agagcgtcga ggaactcgat caggccgcgc
     6961 tcggccgagc tcagatattt cgacgcccgc ggcagcggcc gctcgccgat cgctccggcg
     7021 tccttcgcgg cctcgatcag ggccgggtgg acgtaggatt tccggctgat cgccgcggtg
     7081 ttgccgagcg cttcggcgac cggctcgatg acgctcttca gcttgacgct gccgtgcttg
     7141 cggtagcggc ggatcatctc ctcgaaggcg atggtgctgg cgccccaggt gcggaagtcc
     7201 ttcgccgtga acgcgtcgcc catcgcctcc ttgatatagg cgttgacgtc gccggacgtc
     7261 acgggacaga cgccgccgtc gtcattcaca tattcgaaca gattctgccc aggcaggtcc
     7321 tgggttcgcc gggctatccg gacgaggttc cgatcggtga gggtgacggt gcgctttacg
     7381 ccgtgcttgc cggtatagct gatcttcagc cggccccgtt cgaccctggc gtggcggttg
     7441 cgcagggtcg tggcgccgaa gctcttgttc tccttggcat attcctcgtt gccgacgcgc
     7501 atcctggtct cgtcgatcag ccggaccacc gccgcgagca ccgtctcgcg ggccgtgggc
     7561 ttgccggtca gatcctcttc caccttcttg cgcagcttcg gcaaagcccg gccgaaggcg
     7621 gcgagccgtt catatttggc cgcctcctgc tgcatccgga aatcggggtt gtagcgatat
     7681 tgcttgcggc ccctggcgtc gtagccggtc gccaggatgt gaccttccgg cttgggacag
     7741 aaccagcagc gctcgtaggc gggcggcatt ccgacggcgt tgagccgctc gatctcgtcc
     7801 cggtccgtga tccggctgcc gtcgggcgcg aaatattgcc agtagcggcc ttggcgcttg
     7861 cgggtgatgc cgggcaggtt ccggtcgtcg acgaagcaga gcatgggacg tgtaatcgcc
     7921 cgctggtgcg cttcgttccg ggaacgcggc tcgacgcctt ccgttgaggg cgccagagga
     7981 ggacttcatg cccaacatgt ccgaagcgaa gatcctgatg atcgccaccc atggctatga
     8041 ggattcggag ctgctcgagc cgcggcggat ccttcgcgag cgcggtgccg acgtcaccct
     8101 cgcctcgctt gagaaaggcg agatcgaggg cgacaacggc gccacggccc gggtcgaccg
     8161 cacgatcggc gacgtgaacg cgggcgatta cgacgccctg gtcctccccg gcggcaccaa
     8221 gaatccggac aagctcagga tggacgagcg ggtggtggcg acgatccgcg cctttgccga
     8281 cgccggcaag ccggtcgccg cgatctgcca cggcccgtgg ctgctcatcg aagccgacct
     8341 ggtccgcggc cgaaggatca ccggctggcc ctcgatccga accgaccttc gcaacgccgg
     8401 cggcgaggtg gtcgacgagg aggcggtgac cgacggcaat atcgtcacca gccgcaagcc
     8461 ggaggacatt cccgctttta ccgaggcgct gatcgcgctc gtcgaaaagg tcgaggcgcc
     8521 gctcgaaacc gcctgaggcc atccgtcatc ccggcgaaag ccgggacctc aggacgagaa
     8581 ggctcggcgg gtctccccga ggtcccggct tccgccggga agacggctta ttccgcagcg
     8641 gagagcaacg gccgccgcgc caggcggcgg tcgaacaggc ggttcagcgc ccgctgggcc
     8701 ggccgttcga cgccgtgata gagggccacg gacgcgagga aggtgagcag caggaagaac
     8761 ccggccagga gcggcggaac gttcgccgga tcctcgacga acgcgatctt gaagacgatg
     8821 tagagcagga aatggacgag ataggtcgag tagctgatct cgcccagata ccgtatcggc
     8881 cgggcggcga gcggattgcc gggacggttc gccgtcagag cgagggcgag cagcaggccc
     8941 gccaggaaca gcggcacgac caaagtctcc ggagcccccg cggcgaagcc caggagcagc
     9001 gccgcggcga cgagtgcgcc ggagagtgct gcagcggcgc gcggcccgga gcaccagcgc
     9061 ctccataagg cgcagataat ggtccccatg gtgaactcgg tgacggcgcg gagcaggccg
     9121 aaacggggaa tgtcgcggtc gaggatggtt gcgccgttcc atcccatgac cgccgaaagc
     9181 aggatggcga agaggagaag ggtggcgatc gaccaaaccg gcgagagcct gcgccaatcg
     9241 accgcaagga cgatgagcgg gaagagcaga taggcggcgg cctcgccgct gatcgaccag
     9301 ctcgggtcgt tccaggtcag ggcgctcgtg aacccccaat tgtggacgag caagacatgg
     9361 agtgggagtt ccgcccaggg ataatggacc gtgttgagcg tgcccgtagc ggccaccagc
     9421 gatgcgtaga cgacggtcgc agccaggatg aagaggtgga gcggccagac cctcgcgacc
     9481 cggcgggcga gatatttggg cgcggccgcc agaccgtcgc ggcggaggcg atcggaatag
     9541 ttcagccaca ggacgaagcc cgacagcatg aagaagagat cgacggcgag atagcccttg
     9601 gagaggagga agctcaccct ctccggcagg tacgccacgg cgccgaccct gatgtggtag
     9661 agcaccacga accaggcggc gaggccccgg attccggtca gcgccggaag gtcggcgcgc
     9721 gggtcggccg gagcgccgct catgccggag cggcctcggt ctgcccgctg gccgcggccg
     9781 gggcgaaggc ggcgcggggc cgctccgccc gctcgcggcg gccgatgagg ttgtcgaggc
     9841 cgatctggac tccgaggatc atcagggtga agggcgtgta ggcgatggcg acgaacgagg
     9901 cgccgaccag atagatgatc tggaaatgct ggagagccgt cgccagcggc gcgatccagg
     9961 ccttgtcgcc ctccgactgg cggtagcggc gccggatcac ctccatccgg accagccccc
    10021 cgatgtggat cagaaggaag aggatgaggc ccgggaaacc ctgctcgccg agcatctcga
    10081 aataggcgct gtgccaggcg cgcccctggt cgatttcggt gcgcgcggct atggtctgga
    10141 caccgccctg ctcttgggtg gtgacggtcc ggacctggat cttgttgccg agataggcgc
    10201 cgaagccgcc gcccagcgga tgctcctggg cgtaattcca ggtccagccc cagaccgcga
    10261 gccggacctt ggccgacgaa tccccctcat agccctggat ggtcgacatc cggccggtga
    10321 agctgtcggg caggaacggc accgaggcga cgacgagcag gcccgctccg gcgagataga
    10381 ggaagcggcg cttgacgtcg cgaagggtca gaaggccgag cacggcgatg cagacgaggc
    10441 cggtccgcgc ctcggtcccg atcggcatca gcaggcaggc gaagatcagc ccggcggcga
    10501 acatcttcac ccgccagtcg ggcgggaaga ccgtcccgaa cttggccagc cagaggatga
    10561 tcgggatgag cgcgatcgcg atcgtcgata tggtgctgct ctcgtagagg ttggaattgt
    10621 tggtcaccat caggttgagg acgccgtagc cgccgcccga ggccacggtc ttcagccctc
    10681 cgacgatgat gatcgcggcg gccgacaggg tcaggaacag gagcagggcc tcgatccgaa
    10741 gcttcgagcg cagggtaagc ggaaggaaga tggcgaaggc gagcgaggtc gacgcccagt
    10801 cccatttctc cgccgccgcg atcgggaagg cggcgttcga ggtcgtgtag aagctgtaga
    10861 cgagcaggat cgccagcagc ccctggcgcg ggccgaagcg aaggccggct ttcttctcga
    10921 acagcagcca gcccgcgaag gcgaagcagg cgacgtacat cgacagcggc aggctgttta
    10981 ggaggtaata ggaaaggcgc tgcggagcga ccgtgtcgat ataggcgtag accagcacga
    11041 acaggaacgg ccgccgcaag ccgagcgcga gcacggccag gaggaagccg acgaaaaaga
    11101 ggtcacgcat cgggcttgcc tctgcccagc gtccagcggc ggattttcga ggctgccggc
    11161 cccgttccgg gcgccggatc ctcgtccagg tcggtgcggg agagcaggcg ccaggcgacg
    11221 agcagaagca ggccgtgcgt gagggcgagc gagaaattgt cgatcatcgg agccgtccct
    11281 gcccgcgctt catcccaagc tccctagaga agcgcggttg acgggccgtt aagggtggtt
    11341 acgtccgtcc ttaattcctc ccccggattt ccgggggagg gggaccgcgc gaagcgcggt
    11401 ggaggggttc ttcgaactca gagaagcccc tccaccatgc ttcgcatggt ccccctcccc
    11461 tgcaaaagca ggggaggaat tttgacccac catcacagcg tccgttcgtg cacagcattg
    11521 gcccatgctt gctgggccat ccgggcggcg gttcgaacgg cgccggagcg gagcggaaac
    11581 gcctccgcgt cgagccgcag atcggcgagg tcggagaaga aggctgtcgg cagccgctcc
    11641 cgatgtgcgg cgagaaagtc gcacagtttc tcgaagcggc ggaccagcgt cggattgggc
    11701 cgcttgccgc cgcgggtggc gagctcgaag ctgtggccga cgatattgac caccggatgc
    11761 cgctcgccgg ccgcgtgaag caaggcggcg cgcatctcgt cgaacgagac cgcgcagatc
    11821 tggaggtggc ggaggctgcc gggccgctgc tcgatatggg tgatcggaat ctcgaccagg
    11881 ccctccagca acgccggagc gatccggcgc gggtcgagcg gcaatgcgct cggccaggga
    11941 tggtggctgc cattgtggct gctgtcatag ccgattccga gcttcgccag cgcctcgaga
    12001 gtcgccggat cggcggcata gcttccggag cggaaggcga tgggcggcgg ggcgccggcc
    12061 tcgaccagaa ggtcgcgggc ggtgcggatc agatcgagct ggctccggcg gtcgagaccg
    12121 gtcagctcga agacgggatt ggccgggtcc gcccaggagg gatggaggtg gagctggacc
    12181 tcctggcccg ccgccaggat cggctcgacc atgagccgga ccggctcgat gccgtagagg
    12241 agggccggca tcggatcgac gaagaagcag gccttgaggc cgtgggccgc gagccggtcg
    12301 agctggtagc ttacgcccac gcccgccgga tcgaagctga gcgccagatt ctcgcgccag
    12361 gaggcgccgc tcagatgcca gtcccacctc agctcggtgt cgaccgtcag caggacccgg
    12421 gtcgccatgt cagcgccggc cgaacgtttc gatcttcggg ccgagccggt tgccccggat
    12481 cgccagccgc tcgccgctcc agtcgccgac gaaagtcgtc gtccagcgga agcccggggc
    12541 gaccgaagcg acattgtcct cgatcaccag cccggcggag cggttcttca ccccttccgg
    12601 cgcgaccgag atcatggtgg cgtaattctc cttgccggtg ccgtggacga agacattgcc
    12661 ggcgatccgg ccgacggcgc cgttggagag atcgatctga tagttggtcc gccggccccg
    12721 gctgtcgtcg aagctgttgt ccagcacctc gattcgcggc gcccggctct tgacgtaatg
    12781 gccgcccctg ccctgctcga aacgcgagtt ggtgaccctg aggctgccgt aatggccgat
    12841 atagatgcta tggaccccat cgccctcgcc gaggccgctg aaggtcgaat ggtcgaccga
    12901 gatggagccc gggggtccgt cgtgggagag gatgccggac tggccgtccg agaagaaggt
    12961 gtaggagacg tcgaggtccc cctcctgcag tcggatgccg gcgccgttgc cgtccggaac
    13021 ccggacccgg gtgaagacca gcccgtcgac ccgggcgccc ttgcctcgaa gcacgagagt
    13081 cgccttgccc tcgcaggcgc cgccgtcgaa gaccgcctcg ccgcgcctct cggcgacgta
    13141 ggcgacccgg ccggcctcct ggaccgcgca ttcgccgtag cggcccggag cgatccggat
    13201 cgtcccgctg cctcggccga tcgcgttcac ggcctcctgg agcgagccga aggcctcgcc
    13261 cgtctcagcg agggtgaaag ggccggaagg ctgcgcttgg agcggcccga tcgcggccgc
    13321 cgcggcggca agggccaggg tgaagcgggg tttcatacgg caagctgtta ggccggagcg
    13381 gttgacgggc cgttaagctg attgtggcag agcgctcctc atgcgcatcc tccacgtgct
    13441 cgaccacagc ctgccgctcc acagcggcta caccttccga acgcgcgcca tcgtcaaggc
    13501 gcagcaggcg aaggggtggg aggtcgcctg cctcaccggc gccaggcaca gcgctccggg
    13561 ttccgatccg gagacgctga acggtatcga tttctatcgt acgccgcatc ctccggcccg
    13621 gccgtccccg cttcgggaat ggcaggagat cagggcgcta tcggcgcggc tggacgcgct
    13681 cgtcaccgca tggaagcccg atcaattgca cgtccactcg ccggtgctga acgcgctcgc
    13741 ggcgctgggc gtagcgaagc gccgaggcgt tccgctggtc tacgagatcc gcgccttctg
    13801 ggaggatgcg gcggtcggca acggcacggg gagcgaggga tcggcgcgct accgcctgat
    13861 ccggatgctc gaaacccgtg ccgcgcggca ggccgacgcg gtggcggtga tttgcgaagg
    13921 gctgcgcaag gacctcgtcg ccaggggcat cgaggcggac aagatcatcg tcgcgccgaa
    13981 cggcgtcgac atgacgctgt tcggaaaccc gctcgcgccc gaccgggaca tcgcccgcaa
    14041 gctcgggctc gccggagcgg atgtcgtcgg attcatcggc tccttctacg attatgaggg
    14101 gctggacgat ctcatcgccg ccatgccgct gctgatcgcc aagcggccga aggcgcagct
    14161 gctgctggtc ggcggcggac cgatggaaga ggcgctcaaa gcgcaagcgg aggcgtctcc
    14221 cgccgcggaa cggatccact tcatcggccg cgtgccgcat gaggaggtcg accgctatta
    14281 cagcctgatc gacgtgctcg cttatccgcg caaggcgatg cggctcaccg aattggtgac
    14341 gccgctgaag ccgctcgagg cgatggcgca gcgcaagctc gtggtggctt ccaacgtcgg
    14401 tgggcatcgc gagctgatcg aggacggagt caccggaagc ctgttccggg ccggcgatcc
    14461 ggaggcgctg gcggatgcgc tcgacgccct tttcgccaat cgcggcaagt gggagcaaag
    14521 gcgcgacgtc gcccgccgct tcgtcgaaag agatcgtaac tggtcgtcaa atatttcgcg
    14581 ttacgttccc gtttatcaaa gattgaccgg caaggctctg tagatggcga agaaagttca
    14641 gaagggcgga ggctccgcgc tcgacctcgc aatggcggcg ttcgccggca cggcggcagg
    14701 cttcgtcatg ttcgcgatgc cggactggca gttcgaccgc gcggtggagc ttagcggcct
    14761 gccgctcatc ctcccggccg cgcagccgcc gctcggaatg acggcgcggc tcgccgccat
    14821 cgccgccgcc gcgatcggca cctggatcct cgtctggctc gtgcttcgcg cgctgagcca
    14881 gaagccgccg gagccgaaga gccgtcccaa gcctgtcgag atcgacgtgc cgctgccgcg
    14941 cctccgccgc gccgacgccc atccggacgc ccccgcgcgc cggccgatcc tcgccgcgct
    15001 cgagctcggc gcgccgctca acgcggagcc ggaggccgag caagagggcg aagagacaat
    15061 cgttgaggcg gtgaaggaag aggaggctcc cgaggaggaa atcgtcctcg acagtccgct
    15121 ccctagcttc atcgccgaag cggaagcgac cgaagagttg gaagaagcgg accctgacga
    15181 ggatccggaa atccccgaga ttcgcgattc cgtcgctccc gatggcccgg ccggcgagca
    15241 tgagggggca agcatcgccc atctgatgca gcgcctggag ctgggcctgc tccgccggga
    15301 acgcaccacc tgggacggtc cgacaggcga gcctcggcag aatgaagcgc cttcgcccca
    15361 gctcgaccag cgccttcgca gcgcgatcga cgatctgcag cggctcgccg cccgcggcgg
    15421 ctgatcaggc atcctcgacg gtcagcgcct cgaggctgat ccgcacccta tcctccccgt
    15481 cccgccgctc ttcggagatc aaggcgatat cggccatgat gtggccgcgc agatcgaatt
    15541 ccctggcctc caggtgccgg atgaaatgat cggcggcctc ggcggcgcct tcgccggaga
    15601 ctcggaaggt gagctcgtgg cgtgcgccgg cgaaggtgac gcttgcccag ctgcggcttc
    15661 gtatctcctc gaaatcgacc gcgaagttca tgaagttcat aattatcgcg cgaatgaccg
    15721 cggtggtggc gtcgttgccg cgcttcatcg gccggaccct ccctcgcggg gctccagata
    15781 ggcgcagacg cggttgacga tgcgctggcg gggttcgcgc cccttgcgca gatcgaagac
    15841 gaagcgcgga tcgcgcaccg cctcgcggcc gaagcgggtc ggcgccatgc cgctgcgcct
    15901 caaatagact tccacgcgtc gaagcaggtg cacgttccct tctccttccg aatcagctat
    15961 gttcctgtta tgttcactgt cgaagggcat ttcctacttg tctaggaaaa ttcctatcat
    16021 tatggagcag acatggctga agcggatccg cgcgccgcat tgcagcggct gatcgaggag
    16081 aggcgggagg attatgccgg cctctcgcgc ctgatcgggc gcaacgcggc ctacatccag
    16141 caatatgtga agagggggac gcccaagcgg ctggccgagg aggatcggcg gctgctggcg
    16201 cgctatttcg gcgtggcgga gacgatgctg ggggcgccgg aggcgccggc gacgcgcggc
    16261 gatgcgccgg tggtggtgcc gcaattcgac ttgggcgcct cggcggggcc gggaacgctg
    16321 ccggaggacg agcgggcgcg gtcgcacctt tccttcgacg ccaagtggct gcgccgcctg
    16381 gcgggcgatc ccaagcagct gtcgatgatc aaggtggagg gggattcgat gctgcccacc
    16441 ctgtccgacg gcgacgagat attgg
//
LOCUS       DASWNU010000327         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_195340, whole genome shotgun sequence.
ACCESSION   DASWNU010000327 DASWNU010000000
VERSION     DASWNU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195340"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VGB57_06565"
     CDS             complement(<1..609)
                     /locus_tag="VGB57_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0131053.1"
                     /translation="MRPDPTRCLLLIDDEPAQRRLVTAIGARAGWWVRGAPDVDAAAR
                     ILTDVDGPPVDAILLDHWLPGPDGTGVIQQIRALRPDLPLLVMTAQTSVAVAVEAMRA
                     GAHDFLVKPIAPDSLLAALNAATDRRQRGGGELRPLSEKISNRPLAFEEIVGSAPPFR
                     SALAVAAKAARARVSVLIEGESGSGTEVIAQAIHTASPRGRKP"
     gene            736..873
                     /locus_tag="VGB57_06570"
     CDS             736..873
                     /locus_tag="VGB57_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEY0131054.1"
                     /translation="MAAEGEIEKEVITHRKGYEGFLFLMKWGAIISIVLGFIVVLLIR
                     E"
     gene            887..2014
                     /locus_tag="VGB57_06575"
     CDS             887..2014
                     /locus_tag="VGB57_06575"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY0131055.1"
                     /translation="MKLAILKETADAERRVAATPETVKKFVALGAEVAVETGAGLTAS
                     IADSDYEGAGATVAPREAALAGADAVLGVQGPDPAALQGAKPGALVAAMLDPFRQRER
                     IDAYAVAGLEALAMELMPRITRAQSMDVLSSQANLAGYKAVLDAAAEYGRAFPMMMTA
                     AGTIPAARVFVMGVGVAGLQAIATARRLGAIVSATDVRAATKEQIESLGAKAIFVDKV
                     AGIEGEGAGGYATEMSDEYKAAQAELVSGHIARQDIVITTALIPGRPAPRLISDAQLA
                     SMRPGSVVVDLAVEQGGNVEGSAAGDVAVRHGVRIVGHRNVPSRLAADSSALYARNLY
                     NFLSAFWDKEAGRPLLPDEDEIVKGVRLTQSGKVVHERLLG"
     gene            2208..2576
                     /gene="rpsM"
                     /locus_tag="VGB57_06580"
     CDS             2208..2576
                     /gene="rpsM"
                     /locus_tag="VGB57_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY0131056.1"
                     /translation="MARIAGVNIPTNKRVEIALTYIHGIGRTKAKQIAEKLGIAPERR
                     VQDLTDQEVLHIRETIDADYQVEGDLRREVAMNIKRLMDLASYRGLRHRKGLPVRGQR
                     THTNARTRKGKAKPIAGKKK"
     gene            2589..2978
                     /gene="rpsK"
                     /locus_tag="VGB57_06585"
     CDS             2589..2978
                     /gene="rpsK"
                     /locus_tag="VGB57_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY0131057.1"
                     /translation="MAQAPTRIKRRERKNITAGVAHVNASFNNTMITITDAQGNAIAW
                     SSAGMMGFKGSRKSTPYAAQVAAEDAGKKAAEHGVRTLEVEVKGPGSGRESALRALQS
                     VGFQITSIRDVTPIPHNGVRPSKRRRV"
BASE COUNT          512 a         1053 c         1053 g          425 t
ORIGIN      
        1 cggctttcgg ccgcgcgggg aggcggtgtg gatcgcctgg gcgatcactt ccgtgcccga
       61 gccgctctcg ccctcgatca ggaccgagac ccgggcccgg gccgccttgg cggcgacggc
      121 gagggccgag cggaagggcg gcgccgagcc gacgatctcc tcgaaggcga gcggccggtt
      181 ggagatcttc tcggaaagcg ggcgaagctc gccgccgccg cgctggcgcc ggtcggtggc
      241 ggcgttgagg gcggcgagaa ggctgtcggg agcgatcggc ttgaccagga agtcgtgggc
      301 tccggcgcgc atcgcctcga ccgcgaccgc gaccgaggtt tgggcggtca tgacgagcag
      361 cggcagatcg ggtcgcaagg cgcggatctg ctggatgacc ccggtgccgt ccgggccggg
      421 cagccagtga tcgaggagga tggcgtcgac cggcggcccg tcgacgtcgg tgaggatcct
      481 ggccgccgcg tcaacatcgg gcgcgccccg cacccaccag ccggcgcggg cgccgatggc
      541 ggtgacgaga cgccgctggg cgggctcgtc gtctatcagc agcaggcatc gcgtcgggtc
      601 cggccgcatc gtcactcccc atggctcatg gacgggctgt agcgggcgca ggtaaagttc
      661 ggcttaatca gggcttcaac ggccgccggt ccggcgctag aagggcggcc ggccacggac
      721 agggaaacag gaaatatggc ggcggaaggc gagatcgaga aggaagtcat tacccaccgc
      781 aagggctatg agggcttcct cttcctgatg aagtggggcg cgatcatctc catcgtcctc
      841 ggcttcatcg tcgtcttgct catccgcgaa tagcgcggaa cgcgcggtga agctcgcgat
      901 cctgaaggag acggccgacg ccgagcgcag ggtcgcggcg actcccgaga cggtgaagaa
      961 attcgtcgct ctgggggccg aggtcgcggt cgagaccgga gccggcctca ccgcttcgat
     1021 cgccgattcc gattatgagg gcgcgggagc gacggtcgca ccgcgcgagg cggcgctcgc
     1081 cggcgccgat gccgtcctcg gcgttcaggg gccggatccg gcggcgctcc agggcgcgaa
     1141 gccgggcgct ctcgtcgcgg cgatgctcga tccgttccgg cagcgcgaac ggatcgacgc
     1201 ttatgccgtc gccgggctcg aggcgctggc gatggagctg atgccgcgaa tcacccgggc
     1261 ccagtcgatg gacgtcctct ccagccaggc caatctcgcc ggctacaagg ccgtgctcga
     1321 cgcggcggcc gaatatggcc gggccttccc gatgatgatg accgcggcgg gcaccatccc
     1381 ggccgcgcga gtcttcgtca tgggcgtcgg agtggccggc cttcaggcga tcgccacggc
     1441 gcggcgcctg ggcgcgatcg tctccgcgac cgacgtccgg gcggcgacga aggagcagat
     1501 cgagagcctc ggggccaagg cgatcttcgt cgacaaggtc gccggaatcg aaggcgaggg
     1561 cgccggcggc tacgcgaccg agatgtcgga cgaatataag gccgcgcagg ccgagctcgt
     1621 ctccggccat atcgccaggc aggacatcgt catcaccacc gcgctcatcc cgggccggcc
     1681 ggcgccgcgg ctgatctccg acgcccagct cgcctcgatg cgccccggca gcgtcgtcgt
     1741 cgatctcgcc gtggagcagg gcggcaatgt cgagggctcg gcggccggtg acgtggcggt
     1801 acgccacggc gtccggatcg tcggccaccg caacgtcccc tcccgcctcg ccgccgactc
     1861 ctccgcgctc tacgcccgca acctctacaa tttcctctcc gccttctggg acaaggaggc
     1921 cgggcggccg ctccttcccg acgaggacga gatcgtgaaa ggggtgcggc tgacccaaag
     1981 cgggaaggtc gtccacgaac gcctgctcgg ctaggggaaa aaagccgctt tccgtccttc
     2041 gcttgccgtt gacagcgccc ctctcaaatc ctatctcgcg ctcatcccga taaccggtcg
     2101 tccggcggcg ctttcgtgcg ctcgtcggct gtcgctgttt aagggcccat cgaagcgtcg
     2161 agatggggcg gcagccatcc cgccccgtgg agcaggagat agattttatg gctcgtattg
     2221 ctggggtgaa catcccgacc aacaagcgcg tggagatcgc gctcacctac attcacggaa
     2281 tcggccggac caaggccaag cagatcgccg agaagctcgg aatcgctccc gagcgccggg
     2341 tccaggacct caccgaccag gaggtgctcc atatccgcga gacgatcgac gccgactacc
     2401 aggtcgaggg cgaccttcgc cgcgaagtcg cgatgaacat caagcgcctg atggacctcg
     2461 cctcctaccg cggcctgcgc caccgcaagg gcctcccggt ccgcggccag cgcacccaca
     2521 ccaacgcccg cacccgcaag ggcaaggcca agccgatcgc cggcaagaag aaataaggac
     2581 cggacgaaat ggcacaggca cccacgcgca tcaagcggcg cgaacgcaag aacatcaccg
     2641 ccggagtggc gcacgtcaac gccagcttca acaacacgat gatcaccatc accgacgccc
     2701 agggcaacgc catcgcgtgg agctcggccg ggatgatggg cttcaagggc tcgcgcaagt
     2761 cgacgcctta tgccgcccag gtcgccgccg aggatgccgg caagaaggcc gccgagcatg
     2821 gcgtccgaac cctcgaggtc gaggtcaagg gccccggctc gggccgcgag agcgcccttc
     2881 gcgccctcca gtcggtcggc ttccagatca cctcgatccg cgacgtcacc ccgatcccgc
     2941 acaacggcgt ccgcccctcc aagcgccgcc gcgtctgatt cgaacgggcc ggcgcgctcc
     3001 ggcgcgccgg ccgttccgaa atgccaagac cgtcctgcga tgg
//
LOCUS       DASWNU010000328         5001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_246249, whole genome shotgun sequence.
ACCESSION   DASWNU010000328 DASWNU010000000
VERSION     DASWNU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246249"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1182
                     /locus_tag="VGB57_06590"
     CDS             115..1182
                     /locus_tag="VGB57_06590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY0131058.1"
                     /translation="MAVNAKNWQEMKKPNALEKRSTGGDSRRRATFVAEPLERGFGLT
                     LGNSLRRVLLSSLQGAAVTSIRIEGVLHEFSSLAGVREDITDLVLNVKQIALRMEGDQ
                     PRRLALSATGPGEVTAGQIATTGDIEVMNGDLVLCHLDDGATLNMELTAEAGKGYVPA
                     ASNRPADAPIGLIPIDALYSPVRQVSYKVENTRVGQELDYDKLTLTVETDGTITPDDA
                     VAYAARILQDQLQLFVHFDDSLAAHVPSHGHGHGPSAAETEQDANQLNRYLLKKVDEL
                     ELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLSSMGLRLGMDI
                     PGWPPENIEEMAKKLEQELLG"
     gene            1321..1524
                     /locus_tag="VGB57_06595"
     CDS             1321..1524
                     /locus_tag="VGB57_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131059.1"
                     /translation="MSKASNKPVGRSRVLGSKAFAAISAVEGLKLSRDSSARLDTLRS
                     SSLGGDERRSAVGEAYRDLRTSK"
     gene            1804..3342
                     /locus_tag="VGB57_06600"
     CDS             1804..3342
                     /locus_tag="VGB57_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131060.1"
                     /translation="MSLRFVLPALMLGTSALCGAALAQPADEAVQAAESENILDDAEA
                     LAIDAKLYAREFGVPFEEALNRMTIMTYGGDVVSQEASLEGADLAGRFYQNKSSDFGL
                     TIATKKAARADKSVSFTPRTKANYGRLNAAAMQERNAARKALHQSAGLSDSEVRAAQA
                     RLAAPVQVKVRYKTGQAHSLAELIAGKDAVVALASQYPEINGASVDEIGNSVRIFATK
                     ALSSDQIRDLQKQAGVPLQVDVLPGGFTATANMRGGSKLYASSTATSSASRSCMTAFG
                     ARSNTLKTSTGAALTGMVSAAHCSDTLHVIADTGANMPLSVPAGHVSDTRGGANQTDI
                     QFMSGVNGNPAGLGTFYYDGTTNLRAVAGTQSRAGTAVGSGTAQSVGTGTAVGSYICH
                     LGQTSPGSTANMQSCGEVISINASLSSNQPTSTAYYTGGSFVAVRNTSSGKGTVLGPT
                     GTGTLRCYQGDSGGPWFAGTIAYGVQSWCAWENGVTNSDRAILVLYTSTDFFAQAGVS
                     IIVP"
     gene            3449..3790
                     /locus_tag="VGB57_06605"
     CDS             3449..3790
                     /locus_tag="VGB57_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131061.1"
                     /translation="MTADSALATYRPHVRPDGVAGSAGARLEGRLAARLGCLVVDVGG
                     GRVFQPVFPAGRVRWEASSERLFIDGAAYRPGDRIALGGGGVGDRQGFARASGASIPQ
                     CGGAELFVVSL"
     gene            complement(3895..4149)
                     /locus_tag="VGB57_06610"
     CDS             complement(3895..4149)
                     /locus_tag="VGB57_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131062.1"
                     /translation="MRPLLLAPLLLLLAACEPEPENIQAKAETMSRQIEERANQLTAE
                     AENGVEAQAAPLENEAAALLNRIAGGSAESNIAAEPANVQ"
     gene            complement(4312..4701)
                     /gene="ssrA"
                     /locus_tag="VGB57_06615"
     tmRNA           complement(4312..4701)
                     /gene="ssrA"
                     /locus_tag="VGB57_06615"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            4776..>5001
                     /locus_tag="VGB57_06620"
     CDS             4776..>5001
                     /locus_tag="VGB57_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0131063.1"
                     /translation="MKRTSLMTVFLVPVAAVSLSGCGVNSIPTAEEEAKARWADVQAA
                     YQRRASLIPNLVSTVRGAAASEERILTRVTQ"
BASE COUNT          904 a         1666 c         1639 g          792 t
ORIGIN      
        1 ccaagcgccg ccgcgtctga ccctgtcggc cgaagcgatg cttcggccgg gggaaaagac
       61 gagaagttaa aaaatacggg aggcggacgc gcctcctaac gccagaggaa ccacatggcc
      121 gtcaacgcaa agaactggca ggaaatgaag aaacccaacg cgctcgagaa gcgctccacc
      181 ggcggcgatt cgcgccgccg tgcgaccttc gtcgccgagc cgctcgagcg cggcttcggc
      241 ctgaccttgg gcaactcgct tcgccgggtc ctgctctcgt cgctccaggg cgccgccgtc
      301 acctcgatcc ggatcgaggg cgtgctccac gaattctcga gtctggcggg ggtccgcgag
      361 gacatcaccg acctcgtcct caacgtcaag cagatcgccc ttcgaatgga gggcgaccag
      421 ccgcgccgcc tcgccctttc ggcgaccggc ccgggcgaag tcaccgccgg ccagatcgcc
      481 accaccggcg acatcgaggt gatgaacggc gacctcgtcc tgtgccatct cgacgacggc
      541 gcgaccttga acatggagct gaccgcggaa gccggcaaag gctatgttcc ggccgcctcc
      601 aaccgcccgg ccgacgcgcc gatcgggctc atcccgatcg acgcgctcta cagccccgtg
      661 cgccaggtca gctacaaggt cgagaacacc cgcgtcggcc aggagctcga ctacgacaag
      721 ctcacgctga cggtcgagac cgacggaacg atcacgcccg acgacgccgt cgcctacgcc
      781 gcccgcatcc tccaggacca gctgcagctc ttcgtccact tcgacgacag cctcgccgcc
      841 cacgtcccca gccacggcca cggccatggc ccgagcgccg cggagaccga gcaggacgcc
      901 aaccagctca accgctacct cctcaagaag gtggacgagc tggagctgtc ggtgcgctcg
      961 gcgaactgtc tgaagaacga caacatcatc tacatcggcg acctggtgca gaagaccgag
     1021 gccgagatgc tgaggacccc gaacttcggc cgcaagagcc tgaacgagat caaggaagtg
     1081 ctttcgagca tggggctgag gctgggaatg gatatcccgg gctggccgcc tgagaatatc
     1141 gaggagatgg cgaagaagct cgagcaggag cttctcggct aaggccatcg tctgaaagtg
     1201 gtactttcag ccggttagag acaggaaggg ccgtcccgaa aggggcggcc ctttccttat
     1261 ggcgagcgcc aacgctcacc aactatggca acctgaagcg tcctgtatca ttctgaggct
     1321 atgagcaaag ccagcaacaa gcccgtcggc cgttcacgcg ttctcggatc gaaagccttc
     1381 gccgccatct cggccgtcga ggggctgaag ctcagccgcg acagcagcgc ccggctcgac
     1441 acgctgcgtt cgagctccct cgggggcgac gagcgccgct ctgcggttgg ggaagcctat
     1501 cgggatctgc gcacctcgaa atgatcgacg tcggctacga cgccttcgac gacccctgtt
     1561 gctacagcgg cacaagcgtc ctccggaaca aggccggcct gcgccacccg gaggaactgg
     1621 accttcgagc ttgagaatcg ttacggcgca gtagctgaat gacggatgat cgtttcggcc
     1681 cggggaggtg agtggggcgc catcgggcga gcgggcaccc cgatcggaaa aatcctagca
     1741 acgtaggaac aaaccaagaa catggccgtt tatatggctc agtctatgga gagagattct
     1801 atcatgtccc tacgattcgt tcttccagcc ctcatgctgg gcacctccgc cctgtgcgga
     1861 gccgcgctgg cgcaacccgc cgacgaggcc gtgcaggcgg ccgaaagcga aaacatactg
     1921 gacgatgcgg aagcgctggc aatcgacgcc aaattgtacg ccagggagtt cggagtgccc
     1981 ttcgaggagg cgctcaaccg catgacgatc atgacctacg gcggcgacgt cgtgtcacag
     2041 gaggcttcgc tcgagggtgc cgacctggcc ggccgcttct accagaacaa gagcagcgac
     2101 ttcggcctca cgatcgcgac gaaaaaggcg gccagagccg acaagtccgt ctccttcacg
     2161 ccccgaacga aggcgaatta cggccgcctc aacgcggccg cgatgcagga gcgcaatgcc
     2221 gctcgcaagg ccttgcacca aagcgccggc ctgagcgact ccgaggtacg agcggcgcag
     2281 gcgagactgg ccgctccagt ccaggtgaag gtgcgctaca agaccggcca ggcccattcg
     2341 ctggccgagc tgatcgccgg caaggacgcg gtcgtcgccc tggccagcca gtatccggag
     2401 ataaacggag cgagcgtcga cgagatcggc aactccgtcc ggatcttcgc gaccaaggcg
     2461 ctgagcagcg accagatacg cgatctgcag aagcaggccg gggtcccgct ccaggtcgac
     2521 gtgctgcccg gaggcttcac ggcgacggcg aacatgcgcg gcgggagcaa gctctacgcc
     2581 agctcgaccg cgacgagcag cgcgtcgcgc tcctgcatga cggcgttcgg cgcgcgttcg
     2641 aatacgctca aaacctcgac cggggcggca ttgaccggca tggtctcggc cgcacactgc
     2701 tccgatacgc tccacgtcat cgccgacacc ggggcgaaca tgccgctcag cgtcccggcg
     2761 ggacatgtca gcgacacccg gggcggcgcc aaccagaccg acatccagtt catgagcggc
     2821 gtcaacggca acccggccgg cctcggcacc ttctattatg acggaaccac gaaccttcgg
     2881 gcggtcgccg gcacccagag ccgggccggc accgccgtgg ggagcgggac ggcgcagagc
     2941 gtcgggacgg gcacggccgt aggaagctac atctgccatc tcggccagac ctcgccgggc
     3001 agcacggcca acatgcagag ctgcggcgag gtgatctcga tcaacgcctc gctcagctcg
     3061 aaccagccga cgtccacggc ttattacacc ggcggttcct tcgtggcggt caggaatacc
     3121 agctccggca agggcaccgt cctcgggccc accggcaccg gcaccctgcg ttgctatcaa
     3181 ggcgacagcg ggggcccctg gttcgccgga acgatcgcct acggcgtcca gtcatggtgt
     3241 gcgtgggaaa atggcgttac caattccgac cgcgccatcc tcgtgctata tacgagcacg
     3301 gatttcttcg cccaggccgg agtttcgatc attgttccgt agcttaccga tcgtcgcggc
     3361 ggctttcgcc gcggcttgcc agccggcctc acaggagcct ccttccccca cggggcaagg
     3421 aggctccgac cttcagggga gtggcggagt gactgccgac agcgcgcttg cgacataccg
     3481 gccccacgtt cggcccgacg gagtcgcggg cagcgccggg gcgaggctgg aaggaaggct
     3541 tgccgcgaga ctcgggtgcc ttgtcgttga cgtcggcggg ggcagggtct tccagcctgt
     3601 tttcccggcc ggccgggttc gctgggaagc gtcgtccgag cgccttttca tcgacggcgc
     3661 ggcctatcgt cccggcgacc gcatcgctct gggcggcggc ggcgtcggcg atcgccaggg
     3721 cttcgcccgg gcgagcggag cgagcatccc gcaatgtggc ggcgcggagc tgttcgtcgt
     3781 ctccctctag gctcgatcct cgcgaaaatc cctggtccgg gcggtcggcc gcaagccggc
     3841 tttgccctgc cggggaccga gcttcccggc gcctgggcgg gcctgtcgcc gttgctactg
     3901 aacgttcgcc ggctcggccg cgatattgct ctcggcggag ccgcccgcga tgcggttgag
     3961 gagagcggcg gcctcgttct cgagcggcgc ggcctgggcc tcgactccgt tctccgcttc
     4021 cgccgtgagc tggttggcgc gctcctcgat ctggcgcgac atggtctcgg ctttggcctg
     4081 gatgttctcg ggctccggct cgcaggcggc gaggaggagg aggagggggg cgaggaggag
     4141 ggggcgcatg gcaccccctt acgtcatccg cggggagtcg ggaatccacc atccgtcatc
     4201 ccggcgaaag ccgggacctc agcgaggtag gccgcgccct tcgccgacga ggccccggct
     4261 ttcgccgggg tgacggtctc ggcgaggttt cgccgggatg acgtggagag aagtggggag
     4321 cttctgttgc ccggtgctcc ccgaaccgct ggaaccgatt ctgttgcccg gttcggccca
     4381 accgcgcttt cgtccttgta acctagtccg tgaggaccgg gttacgcggc aatcgcgagt
     4441 gctacgttat cgttggcact tgtggttatg aacggtttta cggcgcattc aggccgggca
     4501 aaaacagcgc ttttcaacac acgtcgatcc tagttcggcc ccatcagctg ccctgcttcg
     4561 ccaaggctac gcagggtaat tggtggagcc gccgggtacc gcccccgggt ccgctgcgtc
     4621 tattgcacgc tgcactttat caccatagcc gggcgaaccc ggcacgactc atatagggga
     4681 tttccggcga atttaaagtg accttcggcg cacccgttgc gggagcgaag cgaagtccct
     4741 acttgcttgg cgcaagcatg aacgaggggt acgacatgaa gcgcacttcc ctgatgaccg
     4801 tcttcctggt gccggtggcg gcggtttcgc tttccggttg cggagtcaac tcgatcccga
     4861 ccgccgagga agaggccaag gcgcgctggg cggacgtgca ggcggcctat cagcgccggg
     4921 ccagcctgat ccccaacctg gtctcgacgg tgcgcggcgc ggcggcgtcg gaagagcgga
     4981 tcctgacccg ggtcacccag g
//
LOCUS       DASWNU010000329         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_90406, whole genome shotgun sequence.
ACCESSION   DASWNU010000329 DASWNU010000000
VERSION     DASWNU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90406"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..406)
                     /locus_tag="VGB57_06625"
                     /pseudo
     CDS             complement(1..406)
                     /locus_tag="VGB57_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
     gene            542..2107
                     /locus_tag="VGB57_06630"
     CDS             542..2107
                     /locus_tag="VGB57_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0131064.1"
                     /translation="MATAPAPSTARRDRLVIAASSLGTVFEWYDFYLYGLLATIISSQ
                     FFSGVNETTGFIFALAAFAAGFAVRPFGALVFGRIGDLVGRKYTFLVTMAIMGLSTFL
                     VGLLPSYASVGVAAPVALVVLRLLQGLALGGEYGGAATYVAEHAPPGRRGFYTSWIQT
                     TATLGLFAALLVVIGTRTALGEEAFADWGWRVPFLLSILLLAVSLWIRMQLEESPVFR
                     KMKEEGTTSKAPLRESFARWGNLRIVLIALFGAVAGQAVVWYTGQFYALFFLEKTLKV
                     DGATANILIAIALALATPFFVFFGWLSDRIGRKPIILAGCALAALTYFPLFEGLTKAA
                     NPALYEAQRQAPVEVMADSRVCSLQFDPIGRTSFNRNGCDIAKSLLAKAGIGYESVDT
                     EFETGARLKIGSAFVDIPNAASTGEPQSHARWAATAEAEIKASLARAGYPAKADPARV
                     NKPAVVAILFLLVLYVTMVYGPIAALLVELFPSRIRYSSMSLPYHIGNGWFGGFLPTT
                     SGLSIRAGSALAG"
     gene            complement(2020..>2260)
                     /locus_tag="VGB57_06635"
     CDS             complement(2020..>2260)
                     /locus_tag="VGB57_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131065.1"
                     /translation="VDTEFETGARLKIGKAFVDIPNAPSIDAREDHARWVATAEADIK
                     AALARAGYPAKADPARIDKPLVVGRKPPNQPLPIW"
BASE COUNT          307 a          812 c          732 g          409 t
ORIGIN      
        1 cggcgaggta gcgccggccg atctcgaaca gcctcacgct ctgggcgccg cgagcggagt
       61 tgcgcctcgc ggccgagagc agccccggca gcagggaagg ccgcatcgcc ttcagctcct
      121 cgctgatcgg attgtcgagg atccaggccg agcctccgaa cggtgccgct tcctcctcgg
      181 cgacgaagct ccaggtcacc gcctcgttga gcccgcgcgc cgcggcggcg cgtcggaccc
      241 ggcgctcgag cagctgctgc gcggtcgccg tcggccgggc cactcccggc gcccgcggca
      301 gcggggtcgc ctcgaccttg tcgaagccct cgatccgcgc gacctcctcg acgatgtcgg
      361 ccgatccgtc gatgtcgcgc cgccagctcg ggacccggac gcgccaggtg tcgcggcccg
      421 tcatcccggc ggaagccggg acctcgggga gacccgccga gacttcttgt cccgaggtcc
      481 cggctttcgc cgggatgacg ggtacttgcg ctccggctcc ggacgcgtga aacccggcgc
      541 catggccacc gccccagccc ccagcaccgc ccgccgcgac cggctcgtca tcgccgcctc
      601 ctcgctcggc accgtcttcg aatggtatga tttctacctc tacgggctgc tcgccacgat
      661 catctcgagc cagttcttct cgggcgtcaa cgagacgacg ggcttcatct tcgccctcgc
      721 cgccttcgcc gccggcttcg ccgtccggcc gttcggggct ttggtgttcg gccggatcgg
      781 cgacctcgtg ggccgcaaat ataccttcct cgtcaccatg gcgatcatgg gcctgtcgac
      841 cttcctggtc ggtctactgc cgagttatgc cagcgtcgga gtcgcggcgc cggtggccct
      901 ggtggtcctt cgcctcctcc agggcctggc gctcggcggc gaatatggcg gcgccgccac
      961 ctatgtcgcc gagcatgccc cgccgggacg gcgcggcttc tatacgagct ggatccagac
     1021 gacggcgacg ctcggcctgt tcgccgccct gctcgtcgtc atcggcaccc gcaccgcatt
     1081 gggggaagag gctttcgccg attggggctg gcgggtgccc ttccttctct cgatcctgct
     1141 cctcgccgta tcgctttgga tccgaatgca gctcgaggag agcccggtct tccgcaagat
     1201 gaaggaggaa gggacgacat cgaaggcgcc gcttcgcgag agcttcgcgc gctggggcaa
     1261 tctgcgcatc gtcctcatcg ccctgttcgg tgccgtcgcc ggccaggcgg tggtctggta
     1321 cacggggcaa ttctacgccc tcttcttcct cgagaagacg ctcaaggtcg acggcgccac
     1381 cgccaacata ttgatcgcga tcgcgctggc gctggccacg cccttcttcg tcttcttcgg
     1441 ctggctgtcg gaccggatcg ggcgcaagcc gatcatcctg gcgggctgcg cgctcgcggc
     1501 gctgacctat ttcccgctgt tcgaagggct gaccaaggcg gccaatccgg cgctttacga
     1561 agcccagcga caggctccgg tggaggtgat ggccgattcc cgggtctgtt cgctccagtt
     1621 cgatccgatc ggcaggacga gcttcaaccg gaacggctgc gacatcgcga agagcctgct
     1681 cgccaaggcg ggcatcggct acgagtcggt cgacaccgag tttgaaacgg gagcgcggct
     1741 caagatcggc agcgcgttcg tcgatattcc caatgctgcg tcgaccggtg agccgcaatc
     1801 acatgcgcgc tgggccgcga ccgccgaggc cgaaatcaag gcgtccctcg cgcgcgccgg
     1861 ctatccggcc aaggccgacc ccgcccgagt gaacaagccc gccgtcgtcg ccatcctctt
     1921 ccttctcgtc ctctacgtga ccatggtcta cgggccgatc gcggccttgc tggtggagct
     1981 gttcccgagc cggatccgct acagctcgat gtcgctcccc taccatatcg gcaacggctg
     2041 gttcggcggc ttccttccaa cgaccagcgg cttgtcgatc cgcgcaggat cggccttggc
     2101 cggatagccg gcgcgcgcga gggccgcctt gatgtcggct tcggcggtgg cgacccagcg
     2161 cgcatggtcc tctcgggcgt cgatgctcgg ggcattgggg atatcgacga acgccttgcc
     2221 gatcttgaga cgcgctccgg tctcgaattc ggtgtcgacc
//
LOCUS       DASWNU010000330         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_505706, whole genome shotgun sequence.
ACCESSION   DASWNU010000330 DASWNU010000000
VERSION     DASWNU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505706"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VGB57_06640"
     CDS             complement(<1..1095)
                     /locus_tag="VGB57_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0131066.1"
                     /translation="MLSIQEAEARAAALVERAARVGADAADAVNYANASTSVQVRLGE
                     LEDVSRSEAETVSLRLFVGSRSASVSSSDLSDEALAALVERAAAMAREAPEDPWSGLA
                     PEELLMREPGPDVEGDDGGDPSPLELRDRSLAIESAGREVAGIANSEGAGVSAGRNLF
                     ALATSHGFSRGYSTTGYSSSVSLIAGAGSEMQRDYAYHSVRHYAELDDPETLGRGAGE
                     RAVARLGPVKLASGTMPVLFDPRAGNSLLGHLMSAIAGPAITRRTSFLLDKEGELLFG
                     EEVTIRDDPHRRRGLRSKPFDGEGLPTRPLNLIDAGRLTGWLLDSASARQLGRAPTAH
                     ATRSSGAPSVGTTNVHMEPGRLSPEQLIGEV"
     gene            complement(1128..2057)
                     /gene="ubiA"
                     /locus_tag="VGB57_06645"
     CDS             complement(1128..2057)
                     /gene="ubiA"
                     /locus_tag="VGB57_06645"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY0131067.1"
                     /translation="MAGDTHAVGEDDIVPDSERRGLIGALPGPLRPYASLMRLDRPIG
                     AWLLFWPCAWSVALAGMGPNGASLILWLALGAWAMRSAGCVWNDIVDRDLDSRVERTR
                     LRPLASKRAGLRGAGALLALLCLIGLVVLLQLGRSAQIVALASLAPVAAYPFMKRITW
                     WPQAWLGLVFSWGALVGWPAVRGELDPTSLILWAGSIFWVIGYDTIYALQDREDDALV
                     GVKSSALALGRHARTGVAVCYGIAMALWAVALWRVRPDGLALVALLPMAGHLGWQVAR
                     LHPDDGADALAKFRSNRIAGFILFLACLVVGTS"
BASE COUNT          333 a          735 c          715 g          274 t
ORIGIN      
        1 gacctcgccg atcagctgct cggggctgag ccgccccggc tccatgtgga cgttggtggt
       61 cccgacgctc ggcgctccgg agcttcgagt ggcatgggcg gtcggagccc gccccaattg
      121 ccgggccgag gcgctgtcga gcagccaacc ggtgagccgc ccggcatcga tcaggttcaa
      181 gggccgggtc ggcagcccct cgccgtcgaa cggcttcgag cgcaggccgc ggcgccggtg
      241 cgggtcgtcg cggatcgtga cttcctcacc gaacaacagc tcgccctcct tgtcgagcag
      301 gaagctggtg cggcgggtga tcgcaggtcc ggcgatggcg ctcatgaggt ggccgagcag
      361 ggaattgccg gcccgcgggt cgaacaggac gggcatcgtc ccgctcgcca gcttgaccgg
      421 accgagccga gcgacggcgc gctccccggc ccctcgtccg agcgtttccg gatcgtccag
      481 ctcggcataa tggcggacgc tgtgataggc atagtctcgc tgcatctcgc tgccggcgcc
      541 ggcgatcagg ctgacggagg aggaatagcc ggtggtcgaa tagccgcggc tgaagccgtg
      601 gctggtggcg agggcgaaca ggttccggcc cgcgctgacc cccgcgcctt cgctgttggc
      661 gatcccggcg acttcccttc cggcgctctc gatcgccagc gaacggtccc tgagctcgag
      721 cggagaagga tcgccgccgt cgtcgccttc gacgtcgggg ccgggctccc gcatcaacag
      781 ctcctcggga gccaggccgg accagggatc ctcgggcgcc tcgcgggcca tggccgccgc
      841 gcgttcgacg agcgccgcga gggcctcgtc ggacaggtcg gaagaagaca cgctcgccga
      901 acggctgccg acgaagaggc gaagggagac ggtttcggct tcgcttcggg agacgtcctc
      961 caattcgccc agccgcacct gaaccgacgt cgaggcgttg gcgtaattga cggcgtcggc
     1021 ggcgtcggcc cccacccgcg cggcgcgctc gacgagcgcg gcggcgcgcg cctcggcttc
     1081 ttggatgctc agcatccatg ccacctaggg tggatagccc ctgaaatcta ggaggtgccg
     1141 acgaccaggc aggcgaggaa caggatgaag ccggcgatcc ggttggaccg gaacttggcc
     1201 agtgcgtcgg cgccatcgtc cgggtggagc ctcgcgactt gccagccgag atgccccgcc
     1261 atcggcagca aggcgaccag agcgagcccg tccggccgta cccgccatag cgcaaccgcc
     1321 cacagcgcca tcgcgatccc gtagcagacc gccacccccg tccgagcgtg ccggcccagc
     1381 gcgagcgcgg aagacttcac tccgacgagg gcgtcgtcct cgcggtcctg aagcgcatag
     1441 atggtgtcgt agccgatcac ccagaagatc gacccggccc acagaatgag cgaagtcggg
     1501 tcgagctcgc cccgcaccgc gggccagccg accagcgcgc cccaggaaaa gacgaggccg
     1561 agccaggcct ggggccacca ggtgatccgc ttcatgaacg gataggccgc cacgggcgcg
     1621 aggctggcga gcgccacgat ctgggcgctc cgtccaagct gaagcagaac gaccagtccg
     1681 atcaggcaga gcagggccag cagggccccg gcccctctca ggcccgcccg cttgctcgcc
     1741 agcggccgca gccgggtgcg ctcgaccctc gagtcgaggt cccggtcgac gatgtcgttc
     1801 cagacgcagc ccgcgcttcg catcgcccag gcgccaagcg ccagccacag gatcagggat
     1861 gctccgttcg gtcccatgcc cgccagcgcc acgctccacg cgcaaggcca gaagagcagc
     1921 cacgccccga tcggccggtc gagccgcatc agcgaggcgt aagggcgaag cggccccggc
     1981 aatgcgccga tcagcccgcg gcgctcggaa tcggggacga tgtcgtcctc tccgactgca
     2041 tgtgtgtcac cggccat
//
LOCUS       DASWNU010000331         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_428287, whole genome shotgun sequence.
ACCESSION   DASWNU010000331 DASWNU010000000
VERSION     DASWNU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428287"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VGB57_06650"
     CDS             <1..1312
                     /locus_tag="VGB57_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0131068.1"
                     /translation="AGLGVVLLAAKWLVWRRFKYGVRAAEIVIESGILNRNRRSIPFD
                     RVQDVDIERTFLARLFGLAKVRIETGAGGKDEGLLDSVSLAEAHRLRETVRAGRAGAA
                     AAVGTQEAEGPAPRILFAMDPPRLVLSGLFNFSLVYIAGLFALLQTFDGFLPFDIYDP
                     ARWVGLVDEYLPARWTFGAIAAVLFVAAALGAVAGVLRTASRDYGFRLSIEGDRLRRE
                     QGLFTRKEAVISRRRVQLAEVSGGPVRGLFGWSGLSFQTLGAGTDRGGHQSAAPFATR
                     SEIEAVLAEVAPLRLPPPPELMPVSRRHIVRSVAATLLPALAAIAALSYWLPFAPALL
                     AALPLLAVAAALERRFHRYGLADDLLFVSRGVWRRRLWLIPLRNVQSLSLSRGPIQRR
                     LGLATLAIDTAGASMINAPRIVDLRGEAAEALAAEISRRRRSQG"
     gene            complement(1423..2343)
                     /gene="cysK"
                     /locus_tag="VGB57_06655"
     CDS             complement(1423..2343)
                     /gene="cysK"
                     /locus_tag="VGB57_06655"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0131069.1"
                     /translation="MRAGSILETIGGTPHIRVNRLFGDAEVWVKSERANPGGSIKDRI
                     ALSMIEEAERSGALKPNGTIVEPTSGNTGIGLAMVAAVKGYKLILVMPESMSLERRRL
                     MLAYGASFDLTPREKGMKGAIARAQELLSEIEDSWMPQQFENPANIEVHLRTTAQEIL
                     EDFRDSPIDVLITGVGTGGHITACAQILKKEWPELKVFAVEPTLSPVISGGQPGPHPI
                     QGIGAGFIPANLHTDVIDGVIQVDPADAKEMARRAAREEGVLVGISSGASLAAVRQKL
                     DEIGPGKRFLTFNYDTGERYLSVPDFLPEA"
     gene            complement(2467..3621)
                     /locus_tag="VGB57_06660"
     CDS             complement(2467..3621)
                     /locus_tag="VGB57_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0131070.1"
                     /translation="MLIIGWQVYNIAREDGLGVAAASARLGLIGLIQFLPLFALTPVT
                     GWVADRVDRRQIARATIALQVLCAAALAYATWAGTVGLTILYSVAFLLGIARAFSGPA
                     LGALAPNLVPRESLPNAIALSSISWQAGMVAGPGLGGYLYAWVPEASYIGAALLFAVS
                     LLCLFLIGPVPRPEMDRTRHPIRQMVDGLAYVRTNRLVLAAITLDLFAVFLAGATALL
                     PVYARDILHVGPEGLAQLGAAPALGATLTALLFSFRPLKTDVGTKMLGAVIVFGVATI
                     AFGFSTWMPLSLAALFVVGASDMFSVYVRQSLIQLSTPDAMRGRVGAVSQLTISASNE
                     LGEAESGFLAALVGPVVAVVAGGVGAIAVTLLWVRWFPELRRARTFDQPG"
BASE COUNT          584 a         1265 c         1343 g          566 t
ORIGIN      
        1 cgccgggctc ggtgtcgtcc ttctggccgc gaaatggctg gtatggcgcc gcttcaaata
       61 cggcgtcaga gcggcggaga tcgtgatcga aagcgggatc ctcaaccgca accggcgaag
      121 catccccttc gaccgggtcc aggacgtcga catcgagcgg accttcctcg cccgcttgtt
      181 cggcctcgcc aaggtgcgga tagagaccgg cgccggcggc aaggacgaag gcctgctcga
      241 cagcgtctcg ctggccgagg cgcaccgcct gcgcgaaacc gttcgcgcgg gccgcgccgg
      301 cgccgccgct gccgtcggta cccaggaggc ggaggggccg gccccgcgga tcctcttcgc
      361 gatggatccg cctcggctgg tcctgtccgg gctgttcaac ttttccctgg tctacattgc
      421 cggcctgttc gcgctcctcc agaccttcga cggcttcctg ccgttcgaca tatacgatcc
      481 cgcccgctgg gtcggtctgg tcgacgaata tctgccggcc cgctggacct tcggagcgat
      541 cgcggcggtg ctgttcgtgg cggcggcgct gggggccgtg gcgggcgtgc ttcggaccgc
      601 ttcgcgcgac tacggcttcc ggctatcgat cgaaggggac cggctgcgcc gcgagcaggg
      661 cctgttcacc cgcaaggagg ccgtgatctc gcgccgccgg gtgcagctcg ccgaggtcag
      721 cggcggcccc gtccggggac tgttcggctg gtcgggcctg tccttccaga cgctgggcgc
      781 cggcaccgac cggggcggcc accagagcgc ggcgcctttc gccacccgaa gcgagatcga
      841 ggccgtcctg gccgaggtcg cgccgctccg gctgccgccg ccgcccgagc tgatgccggt
      901 gtcgaggcgc cacatcgttc gaagcgtcgc cgcgaccctg ctgccggcgc tcgcggcgat
      961 cgccgccttg tcctactggc tgcccttcgc cccggcgctt ctggcggcgc tgccgctgct
     1021 cgccgtggcg gcggcgctgg agcggcgttt ccatcgctat ggactcgccg acgacctgct
     1081 gttcgtcagc cgcggcgtgt ggcgccggcg gctctggttg atcccgttgc gcaacgtcca
     1141 gtcgctgagc ctcagccgag gcccgatcca gcgccgcctc ggcctcgcca ccctggccat
     1201 cgacacggcc ggagcctcga tgatcaacgc gccgcgaatc gtcgacctgc gtggcgaggc
     1261 ggccgaggcg ctggcggcgg agatttcgag gcggcggcgc tcccaagggt gacgccgtga
     1321 gccgactata ccgacattgc gagcgaagcg aagcaatcca gagccacgcc tggcgcccgc
     1381 tggattgctt cgtcgctcgc tcctcgcaat gacggacgaa aatcacgcct ccggaaggaa
     1441 gtccgggacc gagaggtagc gctcgccggt gtcgtagttg aaggtgagga agcgcttgcc
     1501 ggggccgatc tcgtcgagct tctggcgcac cgcggcgagg ctcgctccgg agctaatgcc
     1561 gaccagcacg ccttcctcgc gggccgcccg ccgggccatt tccttggcgt cggccggatc
     1621 gacctggatg accccgtcga tgacgtcggt gtggaggttg gccgggatga agccggcgcc
     1681 gatgccctgg atcgggtggg gcccgggctg gccgccggag atgaccggcg acagggtcgg
     1741 ctcgaccgcg aacaccttca gctccggcca ttccttcttc aggatctggg cgcaggcggt
     1801 gatgtggccg ccggtgccga ctccggtgat cagcacgtcg atcggggaat cgcggaagtc
     1861 ctcgagtatc tcctgggcgg tggtgcgcag gtggacctcg atgttggcgg gattctcgaa
     1921 ctgctgcggc atccagctgt cctcgatctc gctgagcagc tcctgagcgc gggcgatggc
     1981 gcccttcatg cctttctcgc gcggagtgag gtcgaagctc gcgccatagg cgagcatcag
     2041 ccggcggcgc tcgaggctca tgctctccgg catgaccagg atcagcttgt agcccttgac
     2101 cgcggcgacc atggcgaggc cgataccggt attgccggag gtcggctcga cgatcgttcc
     2161 gttcggcttc agggcgcccg aacgctcggc ttcctcgatc atcgacagag cgatccggtc
     2221 cttgatcgag ccgccgggat tggcgcgctc cgacttcacc cagacctcgg cgtcgccgaa
     2281 caggcggttg acccggatat ggggggttcc gccgatcgtc tcgaggatgc tgcccgctct
     2341 cattattgct ctccttcgta ccggttactc aaattggggt cgtccgcaac ttgcttcaac
     2401 tcccgctatc gattctccat cgccagcgcc agcttgagca cgtcgtccca gccttcgctg
     2461 gggacgtcag cccggctggt cgaaggtgcg cgcccgcctc agctccggga accagcgcac
     2521 ccagagcaag gtcaccgcga tcgctccgac cccgcccgcg accaccgcga cgaccgggcc
     2581 gaccaacgcg gcgaggaacc ccgattccgc ctcgccgagc tcgttcgagg cggagatggt
     2641 gagctgggag acggcgccca cccggccgcg catcgcatcg ggggtggaca gctggatcag
     2701 cgactggcgg acgtagaccg agaacatgtc ggaagctccg acgacgaaca aggcggcgag
     2761 cgacagcggc atccacgtcg agaagccgaa ggcgatggtg gcgaccccga agacgatcac
     2821 ggcgccgagc atcttggttc cgacatcggt cttgagcggc cggaaggaga agaggagcgc
     2881 ggtgagcgtc gctccgagcg cgggcgcggc gccgagctgg gccagcccct ccggacccac
     2941 gtggaggatg tctcgggcgt agaccgggag aagcgcggtc gctccggcga ggaagacggc
     3001 gaagaggtcc agggtgatgg cggcgagcac aagccggttg gtccggacgt aagcgaggcc
     3061 gtcgaccatc tggcggatcg ggtggcgggt tcgatccatc tcggggcgcg gcaccggccc
     3121 gatcagaaac aggcagagga gcgacacggc gaagagcagc gcggcgccga tgtaggaagc
     3181 ctccggcacc caggcataga gatagccgcc gaggccgggg ccggcgacca ttcccgcctg
     3241 ccacgagatg gaggacaaag cgatcgcatt gggcagcgac tcccgcggca ccaggttcgg
     3301 agcgagcgcg cccagtgccg ggcccgagaa agcgcgggcg atgccgagca ggaaggcgac
     3361 cgaatagagg atggtcaggc ccaccgtgcc ggcccaggta gcgtaggcga gggccgcggc
     3421 gcagaggacc tggagggcga tggtggctcg ggcgatctgc cgccggtcga cccggtcggc
     3481 gacccagccg gtgaccggag tcagagcgaa cagcggcagg aactggatga ggccgatgag
     3541 accgagccgg gccgaggcgg cggcgactcc gagcccgtcc tcgcgggcga tattgtagac
     3601 ctgccagccg atgatcagca tcaggcagac gagcgagacg gcgaacagga tcgccgcgcc
     3661 gatataggac gcctccggca cccacgcgta gagatagccg ccgaggcccg gcccggcgat
     3721 catccccgcc tgccaggaga tggaggagag ggcgatcg
//
LOCUS       DASWNU010000332         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_401358, whole genome shotgun sequence.
ACCESSION   DASWNU010000332 DASWNU010000000
VERSION     DASWNU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401358"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..2060
                     /locus_tag="VGB57_06665"
     CDS             219..2060
                     /locus_tag="VGB57_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY0131071.1"
                     /translation="MKLTIGAIGIVFGDIGTSPLYAFRQTFAGLHPLPVDRLHVMGVL
                     SLIFWSMMIVVTMKYILILMRADNKGEGGSLALLALLSRKTERARWGTVIVLLGVFAC
                     ALFYGDTMITPAISVLSAVEGIVLIEAGFEPWVIPIAIGILVGLFSIQRRGTAAVGAL
                     FAPVLIVYFSVLAVLGLASIALHPEIVGALNPWWAVQFFLEERFFAFVAMGSVVLAVT
                     GTEALYADMGHFGRRPIQFAWFFFVLPALMINYLGQGALLIADPGTISSPFFMLAPDW
                     FRLPLVVIATMATVIASQAVISGAFSVTQQAIHLGYIPRLKITHTSATAASQIYIPSV
                     NWALAVAVVVLVLLFRNSDNLSAAYGIAVTGAMFIDTCLLAVVLFALWGWNRWLATLV
                     LALFFTVDTAYFAANLLKVPSGGWVPLAMGLIAFTLLTTWAKGRKLLHRQLKQGDIPI
                     PLFIKSAAASAARVPGTAIFMTSQADGVPHALLHNLKHNKVLHERSIFLKVAIHDVPY
                     VDEERRVEVEDLGEGFYKIVLHFGFMQDMNVPEALKAIDRCGLPFKMMETSFFLSRQT
                     PLTSTVHGMAIWREKLFAWMMRNAESPMEFFGLPPNRVVEMGSQVKI"
     gene            2203..>2535
                     /gene="wrbA"
                     /locus_tag="VGB57_06670"
     CDS             2203..>2535
                     /gene="wrbA"
                     /locus_tag="VGB57_06670"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY0131072.1"
                     /translation="MAKILILYYSSYGHILEMAEAVAEGARSAGATVDISRVPELVPE
                     EVARKSGYRLDQPHPVARVEDLTRYDAIVVGVGTRFGRMASQMANFLDQAGGLWASGA
                     LNGKVGSAF"
BASE COUNT          380 a          914 c          758 g          483 t
ORIGIN      
        1 gtgttcatgg atcccggccg tcgccgggat gacgagacac ttgctccgcc ctccgtgaaa
       61 tcctagcaat ccccgcggtc cggggccgga acggaccgtg ccttcggcca ttcctaccgc
      121 gcgatgccaa gggaccaagg atgagcgaag cctcggctgc cccggccctc ccggacgcgc
      181 gcgatcccga gcccggccac cagcatcatg gcgggctgat gaagctcacc ataggcgcca
      241 tcggaatcgt cttcggcgat atcggcacct cgccgctcta cgccttccgc cagaccttcg
      301 ccggcctcca tccgctcccc gtcgaccggc ttcacgtcat gggcgtgctc agcctgatct
      361 tctggtcgat gatgatcgtc gtcacgatga aatacatcct catcctgatg cgcgccgaca
      421 acaaggggga aggcggaagc ctcgccctcc tcgccttgct ctcgcgtaag accgagcggg
      481 cgcgatgggg gacggtgatc gtcctgctcg gggtcttcgc ctgcgcgctc ttctacggcg
      541 acacgatgat caccccggcc atctcggtgc tttccgccgt cgagggcatc gtcctgatcg
      601 aggccggctt cgagccgtgg gtgatcccga tcgcgatcgg aatcctcgtc ggcctgttct
      661 cgatccagcg ccgaggcacc gccgcggtcg gggctttgtt cgctccggtg ctgatcgtct
      721 acttctcggt cctcgccgtg ctcggcctag ccagcatcgc tttgcacccc gagatcgtcg
      781 gcgcgctcaa cccctggtgg gccgtgcagt tcttcctcga ggagcgcttc ttcgccttcg
      841 tcgccatggg ctcggttgtg ctcgcggtga ccggcaccga ggcgctctac gccgacatgg
      901 gtcatttcgg ccgccggccg atccagttcg cctggttctt cttcgtcctg ccggcgctga
      961 tgatcaacta tctcggccag ggggcgcttc tgatcgccga cccggggacg atctccagcc
     1021 ccttcttcat gctcgctccc gactggttcc ggctgcctct ggtcgtgatc gcgaccatgg
     1081 cgaccgtcat cgccagccag gcggtgatct ccggggcctt ttcggtgacc cagcaggcca
     1141 tccacctcgg ctacatcccg cgcctcaaga tcacccatac cagcgcgacc gcggcgagcc
     1201 agatctacat accctcggtc aactgggcgc ttgccgtggc cgtggtcgtc ctcgtcctcc
     1261 tcttccgcaa ctcggacaac ctctcggccg cctacggaat cgccgtcacc ggggcgatgt
     1321 tcatcgacac ctgcctgctc gcggtcgtgc tgtttgccct gtggggctgg aaccggtggc
     1381 tggccaccct cgtcctcgcg ctgttcttca ccgtcgacac cgcctatttc gccgccaatc
     1441 tcttgaaggt gccgagcggg ggctgggtgc cgctggcgat gggcctcatc gccttcacct
     1501 tgctgaccac ctgggcgaaa ggccgcaagc tgctccatcg ccagctcaag cagggcgaca
     1561 ttccgatccc gcttttcatc aagtccgccg ccgcctcggc cgcccgggtc cccggcaccg
     1621 ccatcttcat gaccagccag gcggacgggg tgccccacgc gctcctccac aatctcaagc
     1681 acaacaaggt gctccacgag cgctcgatct tcctcaaggt ggcgattcac gacgtgccct
     1741 atgtcgacga ggagcggcgg gtggaggtcg aggatctcgg cgagggcttc tacaagatcg
     1801 tcctccactt cggcttcatg caggacatga acgtgccgga ggcgctgaag gcgatcgacc
     1861 gctgcggtct gccgttcaag atgatggaga cgagcttctt cctctcccgc cagaccccgc
     1921 tcacctccac cgtccacggc atggcgatct ggcgcgagaa gctcttcgcc tggatgatgc
     1981 gcaacgccga aagcccgatg gaattcttcg gccttccgcc caaccgcgtc gtcgagatgg
     2041 gcagccaggt gaagatctga ccattgcgtc atcccggcga aagccgggac ctcatgacaa
     2101 gaacgctcga cgggtctcca cgaggtcccg gctttcgccg gaatgacgga gctggcacct
     2161 cgccgcctcc cacgcgttgc cgcccccgaa gggagccccg ccatggccaa gatcctgatc
     2221 ctctattatt cgtcctacgg ccacatcctc gagatggccg aggccgtcgc cgaaggggcg
     2281 cgctcggccg gggccaccgt cgacataagc cgggtccccg agctggtgcc cgaggaggtc
     2341 gcccgcaaat cgggctaccg gctcgaccag cctcatcccg ttgcccgggt cgaggatttg
     2401 acccgctacg acgcgatcgt cgtcggagtc ggcacgcgct tcggacggat ggcctcgcaa
     2461 atggccaatt tcctcgacca ggccgggggc ctgtgggcgt cgggcgcctt gaacggcaag
     2521 gtcggctccg ccttc
//
LOCUS       DASWNU010000333         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_452354, whole genome shotgun sequence.
ACCESSION   DASWNU010000333 DASWNU010000000
VERSION     DASWNU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452354"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1160
                     /locus_tag="VGB57_06675"
     CDS             72..1160
                     /locus_tag="VGB57_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131073.1"
                     /translation="MRLFSRMFLTAALLLAPCSAAAEPSADERLAIACPSRAGPVDAQ
                     GFVTIGGIEQWVTVRGSACANPVLLFVHGGPGNPMSPFSANVFAGWEQGFTIVQWDQR
                     GAGRTFGRNRAVADAPLSMERMTQDGVELAAYLAPRLGQRRVVLIGTSWGSALGVHMA
                     LARPDLFHAYVGVSQLVHYRANQEGSYRSLLARARAADDSETVAAIATLGPPPWTNPR
                     NFGILRRAIRRYERRATDAAPDGWWIPAPLYATPEAAAAYQAGEEYSFLQFVGAAGDG
                     LFSRLDLPALGRRFAIPVFLVQGSEDLLTTPEVSRRYFEYLTAPVKSFVLVPRAGHDP
                     NPAMLEAVRDILVRQRAAWLAARGDAAN"
     gene            1260..>1752
                     /locus_tag="VGB57_06680"
     CDS             1260..>1752
                     /locus_tag="VGB57_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY0131074.1"
                     /translation="MVAHVATVAFLGLEARAVEVQVQIASGLPAFIVVGLPDKAIAES
                     RERVRAALAAIGLALPPKRITVNLSPADLPKEGSHYDLPIALGLLAAMGAVDKETLAG
                     FVVVGELGLDGRIAPSPGVLLAALHASSMELGLICPAGQGPEAAWAGTIEVIAEPDLI
                     SLLN"
BASE COUNT          243 a          621 c          590 g          298 t
ORIGIN      
        1 caaatcggcg tgcgctttct cgccacctcg ctcccacgct gctacggtct tcttctccgg
       61 gggatgaagc gatgcgcctt ttcagcagga tgttcctcac cgccgcgctc ctcctggcgc
      121 cgtgctcggc ggcggcagag ccctcagccg acgagcgcct ggctattgca tgcccctccc
      181 gggccggccc ggtggacgcg cagggcttcg tcaccatcgg cgggatcgag caatgggtca
      241 ccgtgagggg ctcggcctgc gcaaatccgg tgctgctttt cgtccacggc ggccccggca
      301 atccgatgag ccctttttcg gcgaacgtct tcgccggatg ggagcaaggc ttcacgatcg
      361 tgcagtggga ccaaaggggc gccggccgga ccttcggcag gaaccgggcg gtggcagacg
      421 cacccctcag catggagcgg atgacgcagg acggcgtcga gctcgccgcc tatctcgccc
      481 cgaggctggg ccagcgccgc gtagtgctga tcggtacgtc ctggggttcg gccctcgggg
      541 tgcacatggc cctggcccgc ccggatctgt tccacgccta tgtcggggtc tcgcagctcg
      601 tccactatcg cgccaaccag gaggggagct atcgcagcct ccttgcccgg gcccgcgcgg
      661 cggacgattc cgagaccgtc gccgcgatcg caacgctagg ccctccgccc tggacgaacc
      721 cgcgcaattt cgggatcctg cgccgcgcga tcaggcgcta cgagcgccgg gcgaccgacg
      781 ccgctcccga cggctggtgg attcctgcgc cgctctacgc gacgccggaa gccgccgccg
      841 cctatcaagc gggcgaggaa tattccttcc tccagttcgt cggcgccgcc ggtgacggcc
      901 tcttctcccg cctcgatctg cctgcgctcg gcaggaggtt cgcgatcccg gtcttcctcg
      961 tccaggggag cgaggatttg ctgacgacgc cggaggtttc ccggcgctac ttcgaatatc
     1021 tgaccgcgcc ggtcaaaagc ttcgtgctcg tcccgcgcgc cggccacgat cccaatcccg
     1081 ccatgctcga agcggtgcgc gatatcctcg tccggcaacg cgccgcctgg ctggccgccc
     1141 ggggcgacgc ggcgaactag gtccaatctg gtgccaatcg gggcttccgc accctccgcg
     1201 gaaatgctca acaacggatg ggaattcatc cgaaatggat gaatcgcgag gggggcttca
     1261 tggttgcgca cgtcgccacg gtcgcgtttc tcggcctgga agcgcgcgcc gtcgaggtgc
     1321 aggtccagat cgcgagcggc ctgccggcct tcatcgtcgt cggcctgccc gacaaggcga
     1381 tcgccgagag ccgggagcgg gtacgggccg ccttggccgc gatcggcctc gcgctgccgc
     1441 ccaagaggat cacggtcaac ctctcgccgg ccgatttgcc caaggagggc tcgcattacg
     1501 atctgccgat cgcgctgggg ctgctcgcgg ccatgggggc ggtcgacaag gagacgctcg
     1561 ccggattcgt cgtggtcggc gagctcgggc tcgacggacg gatcgcgccc tcgccgggcg
     1621 tgctcctcgc ggcgcttcac gcctcgtcga tggagctcgg cctgatctgt ccggcggggc
     1681 aggggcccga ggccgcctgg gccggcacca tcgaggtgat cgcggaaccg gatctcatct
     1741 cgctcttgaa tc
//
LOCUS       DASWNU010000334         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_13396, whole genome shotgun sequence.
ACCESSION   DASWNU010000334 DASWNU010000000
VERSION     DASWNU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13396"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="VGB57_06685"
     CDS             complement(<1..1819)
                     /locus_tag="VGB57_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131075.1"
                     /translation="MKRPRPTAFLAALLASALLAAVAATPASAEFGLKEFDVYFSEKD
                     GSPAAQAGQHPFAMVTDITVNTRPDPILGEVPDDEVKDLEIHLPVGFAGNPGATPRCS
                     TEDFVRTRQGEADTAKSSLCPDATAIGEVAAGVEKPGWYYHAPVYNLEPAPGTAAKFG
                     FVVLGVPVTIDATVNPDRPHNITAKLNNINQTLRFYTSRFTLWGNPSDPAHDPVRGSC
                     VAVFEQDQSPATPSRLNPETGSVGCPVTNPKTAFLTMPRSCSGPMDTAYRVESWQNPG
                     VFLQGSSRSHDNSTPPLPLGMTGCEKLGFGPTISAAPTARSAESPSGLDFNLDVTDEG
                     LTNPAGLAQSDIRKAVVTLPRGVTANPSLAEGLGTCTPADYAGEQIGTAGCPQSAKIG
                     TVEVETPLLEGELLRGSVFIATPDDQATATPGAENPFDSLIALYMVIESPELGILVKV
                     PGRVDPDERTGQLVTTFDDIPQFPFSHFRFHFRSGGRSPLVTPPTCGTYQIRAELTPS
                     ARPAETYTTTSEFRITSGVEGGPCPAGGVPPFRPGFEAGSINNNAGSYSPFYMRLSRR
                     DGEQDITRFSATLPPGVTGKLAGIARCPAAAAAAAKAKSG"
BASE COUNT          242 a          530 c          767 g          302 t
ORIGIN      
        1 gcccgctctt ggctttggcc gccgcggcgg ccgcggccgg gcagcgggcg atgcccgcca
       61 gcttgccggt caccccgggg ggcagggtcg ccgagaagcg ggtgatgtcc tgttcgccgt
      121 cgcggcggga gaggcgcatg tagaaggggg agtaggagcc ggcgttgttg ttgatcgagc
      181 ccgcctcgaa gccggggcgg aagggcggca cgcccccggc cgggcagggc ccgccttcga
      241 cgccgctggt gatccggaac tcagaggtgg tggtgtaggt ctccgcgggg cgcgcggagg
      301 gcgtcagctc ggcgcggatc tggtaggtgc cgcaggtcgg cggcgtcacc agcggcgagc
      361 ggccgccgga gcggaagtgg aagcggaagt gggagaaggg gaactggggg atgtcgtcga
      421 aggtcgtcac cagctgcccg gtgcgctcgt cggggtccac gcggccgggg actttgacca
      481 ggatcccgag ctcgggggat tcgatcacca tgtagagggc gatcagcgag tcgaaggggt
      541 tctctgcccc cggcgtcgcg gtggcctgat cgtcgggggt ggcgatgaag accgagccgc
      601 gcaggagctc gccttccagc agcggcgttt cgacttccac ggtgccgatc ttggccgact
      661 gcgggcagcc ggccgtgccg atctgctcgc cggcgtagtc ggccggggtg caggtgccga
      721 gaccttcggc gagcgagggg ttggcggtga ccccccgcgg cagggtgacg acggcctttc
      781 tgatgtccga ctgggccagc ccggcggggt tggtcagccc ttcgtcggtg acgtcgaggt
      841 tgaagtccag gccgctcggg gactcggccg agcgggcggt gggggcggcg gagatggtcg
      901 gcccgaagcc gagtttttcg caaccggtca tccccagggg cagcggcggg gtcgagttgt
      961 cgtggctgcg ggaggagccc tgcagaaaga cgcccgggtt ctgccaggac tcgacgcggt
     1021 aggcggtgtc catcggcccc gagcagctgc gcggcatcgt caggaaggcg gttttcggat
     1081 tggtcaccgg gcagcccacg gatccggttt ccgggttcag gcgcgagggg gtggcgggcg
     1141 actggtcctg ctcgaagacc gccacgcagg agccgcggac ggggtcgtgg gcggggtctg
     1201 agggattgcc ccagagggtg aagcgcgagg tgtagaagcg cagcgtctgg ttgatgttgt
     1261 tgagcttggc ggtgatgttg tggggacggt cggggttgac ggtggcgtcg atcgtcaccg
     1321 gcacgcccag caccacgaag ccgaatttcg ccgcggttcc cggcgcgggc tcgaggttgt
     1381 agacgggggc gtggtagtac cagccgggtt tttcgacccc ggcggctacc tcgccgatcg
     1441 ccgtcgcgtc ggggcagagc gagcttttcg cggtgtccgc ctcgccctgt cgggtgcgga
     1501 cgaagtcttc ggtcgagcag cgcggcgtcg cgccggggtt gccggcaaag ccgaccggga
     1561 ggtggatctc gaggtctttg acttcgtcgt cggggacttc gccgaggatc gggtcgggcc
     1621 tggtgttgac ggtgatgtcg gtcaccatcg cgaaggggtg ctggccggcc tgggcggcgg
     1681 gggagccgtc tttttcagag aagtagacgt cgaactcttt gagcccgaac tccgccgagg
     1741 cgggggttgc ggcaaccgcg gccagcaggg ccgaggcgag cagcgcggcg aggaaggcgg
     1801 tggggcgagg acgcttcatc gggcgctcct tttgccgttg c
//
LOCUS       DASWNU010000335         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_246452, whole genome shotgun sequence.
ACCESSION   DASWNU010000335 DASWNU010000000
VERSION     DASWNU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246452"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGB57_06690"
     CDS             complement(<1..269)
                     /locus_tag="VGB57_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY0131076.1"
                     /translation="MIASLPRREREIFEILCSAGEATAADVRRAMADPPSHSAVRTML
                     ARLEAKGLVRHRAEDQTYVYRSVPQPAKVRETALKALVKTFFDGSA"
     gene            379..1020
                     /locus_tag="VGB57_06695"
     CDS             379..1020
                     /locus_tag="VGB57_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HEY0131077.1"
                     /translation="MNHQLEVRVVARINRDFFFSHVRMALFDGSLKQSQVTGLDAILD
                     HWEARAGAKDDRWLAYLLATAHHETDRTLKPIREYGGTAYFTRRYDPPPTGRNPRIAR
                     DLGNTRPGDGARYCGRGFVQLTGRRNYTDWGRRLGVDLVGNPALALELDIATKILVDG
                     SLLGTFTGKKLADYLHDDVADWRNARRVINGVDKANSIAGYALKYYAAIGYTV"
     gene            complement(1154..1930)
                     /locus_tag="VGB57_06700"
     CDS             complement(1154..1930)
                     /locus_tag="VGB57_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0131078.1"
                     /translation="MGRTVMVMMALAMATAVPAAAKGQASGPAHVAAAVAAPGRPKAA
                     TDLDEVRKPVELLRFMGLKKGDRALDYFTGTGYYAEIMARAVGPSGHVVGWNSASFGR
                     NEKVVAALKELRSRNSNAAFYSSPSTAIAFAPASFDFALLHLVYHDAYWESAQFGLPR
                     IDPNTVVKAVWDAVKPGGTVAIVDHVAVPGGDTRAVVEKLHRIDPAVVKADFERAGFV
                     LEAQSDMLRVREDDHSKNVFDPSIRGRTDRFVYRFRKPAR"
     gene            complement(1950..3539)
                     /locus_tag="VGB57_06705"
     CDS             complement(1950..3539)
                     /locus_tag="VGB57_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0131079.1"
                     /translation="MSLTRRALLERLGLAGGVSAVYLGMEAMGLLNAPPAAAEPFALP
                     PAPRGKGSVVVLGAGIAGLVAAYELRRAGWDVTVLEARDRVGGRVWTVRGGDRIEQMG
                     RPDQVCGFSKGLYLNAGAARIPTAHHVILGYAKRLGVPLEVMVNSNRAAKWDFGGRNV
                     ANRQAVNDVRGRVAELLSKAVRKGALDEELTAVDKSVMVNFLGAYGGLSRPNGDYKGD
                     GRSGYSVLPGAYGEAGTTVPPMKLEEIMKERGWVLPLLFEEIFDQQAPMFQPVGGMDR
                     IAGALYEQVKPAVRLGTPVTAIRRIGERVRIEHGRGKTDADYCICTLGVNLLARIPND
                     FSPAKQAAMKDMAYVPAVKVAFESRRFWEDEGVYGGVGWTDAMSENVLYPSGGWHRDK
                     GVLVAAYVAGWTGQEHPAQFTALSHEERFRICREVIGRMHPGRAGELGRPVTVAWGLT
                     RWSEGVGPGNDAWFESPRPAVYAELYKPEGPIFFAGEHLSYVQFWQEGAALSSHAAMQ
                     ALQARVQERALAERAGRARTA"
     gene            complement(3536..>3811)
                     /locus_tag="VGB57_06710"
     CDS             complement(3536..>3811)
                     /locus_tag="VGB57_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY0131080.1"
                     /translation="AQTRSVLRKIEAQLKQHDMGLGDIVMMRVFLIAPPGQARMDFAG
                     MMTAYREFFGTAAQPGKPARSTLQAAGLVDPGWLVEIEATAAKAKGR"
BASE COUNT          639 a         1358 c         1199 g          615 t
ORIGIN      
        1 gccgacccgt cgaaaaaggt tttgacgagc gccttcagag ccgtttcgcg aaccttggcg
       61 ggctgcggca cgctcctgta gacataggtc tggtcctcgg cgcggtgccg gaccagccct
      121 ttcgcctcga gccgcgccag catggtgcgg accgccgaat ggctgggcgg atccgccatc
      181 gcccggcgca cgtcggcggc cgtggcctcc ccggcgctgc acaggatctc gaatatctcg
      241 cgctcacggc gcggcaggct ggcaatcatc acgactcggt tcactcctgc tacacctgta
      301 gcatgccaca aacgtagcag gttgcaagtg ccgttcgtcg aaattgtagg agacttcgtc
      361 gatcctatag aacataacgt gaaccatcag ctcgaggtgc gcgtagtggc ccggatcaat
      421 cgtgacttct ttttcagcca tgtgcgcatg gccctgttcg acggatcgct gaagcagtcg
      481 caggtcaccg gcctcgacgc gattctcgac cattgggagg ctcgggccgg agccaaggac
      541 gatcgctggc tcgcctacct gctggcgacg gcgcaccatg agaccgaccg caccttgaag
      601 ccgatccgcg aatatggcgg caccgcctat ttcacccgcc gctacgatcc gccgccgacc
      661 ggcaggaatc cgcggattgc cagggatctc ggcaacaccc gccccggcga cggcgccaga
      721 tattgcggcc gcggcttcgt ccagctcacc ggccggcgca attataccga ctggggcagg
      781 aggctcggcg tcgacctggt cggcaacccc gctctggcgc tggagctcga catcgcgacg
      841 aagatcctgg tggacggatc cctgctcggc accttcaccg gcaagaagct cgccgactat
      901 ctccacgacg acgtcgccga ctggcgcaac gcccgccgag tcatcaacgg agtcgacaag
      961 gccaattcga tcgcgggcta cgcgctcaaa tattacgcgg cgatcggcta caccgtttga
     1021 cgaaggaggg tggaagcgcg gcaatgctcc cgtcatcccg gcgaaagccg ggacctcggg
     1081 gagacccgtg gagccttctc gtcccgaggt cccggctttc gccgggatga cgattgcgcg
     1141 ccggcctctt cgcctagcgc gccggcttgc ggaaccgata gacgaagcgg tcggtcctgc
     1201 cgcggatcga cgggtcgaag acgttcttcg aatgatcgtc ctcgcgcacc cgaagcatgt
     1261 cggactgcgc ctccagcacg aagccggcgc gctcgaaatc ggccttcacc acggccggat
     1321 cgatccggtg aagcttctcg accaccgctc gggtatcgcc gccggggacc gcgacatgat
     1381 cgacgatcgc gaccgttccg cccggcttca ccgcgtccca gaccgccttc accactgtgt
     1441 tcggatcgat ccgcggaagg ccgaactggg cgctttccca ataagcgtcg tgatagacga
     1501 gatggaggag cgcgaaatcg aagctggccg gagcgaaggc gatcgccgtc gacggactcg
     1561 agtagaaggc ggcgttggaa ttgcggctcc ttagctcctt gagagcggcg acaacctttt
     1621 cgttgcgtcc gaagctggcc gaattccagc cgacgacatg gccgctcggc ccgaccgccc
     1681 gcgccatgat ctccgcataa tagccggtgc cggtgaagta atcgagcgcc cggtcgccct
     1741 tcttcaggcc catgaagcga agcagttcga ccggcttcct cacctcgtcc aggtcggtcg
     1801 ccgccttggg ccggcccggc gccgccaccg ccgccgcgac gtgggcgggg ccgctcgcct
     1861 gacctttcgc ggccgccgga acagccgtcg ccatcgccag agccatcatc accataaccg
     1921 tcctgcccat ctccgcctcc ctcgcttcct caggccgtcc gggcgcgtcc agcacgctcc
     1981 gccaacgccc gttcctgaac ccgcgcctga agcgcctgca tagcggcgtg cgacgaaagc
     2041 gccgcccctt cctgccaaaa ctggacgtag ctcaaatgct cgccggcgaa gaagatcggt
     2101 ccctccggct tgtacagctc ggcataaacc gccggccgcg gggattcgaa ccaggcatcg
     2161 ttgcccggac cgacgccttc cgaccagcgc gtgaggcccc aggcgaccgt caccggccgg
     2221 ccgagctccc ctgccctccc cggatgcatc cggccgatca cctcgcggca gatccggaag
     2281 cgctcctcat ggctgagcgc ggtgaattgc gcgggatgct cctggccggt ccagccggcg
     2341 acgtaggccg cgaccagcac gcccttgtcg cggtgccagc cgccggacgg gtagagcacg
     2401 ttctcggaca tggcgtcggt ccagccgact ccgccgtaaa ccccttcatc ctcccagaag
     2461 cgccgcgatt cgaaggcgac cttgacggcg gggacgtagg ccatgtcctt catcgccgcc
     2521 tgcttggcgg gcgagaaatc gttggggatg cgggcgagca ggttcacgcc cagggtgcag
     2581 atgcaataat cggcgtcggt cttgccgcgg ccatgctcga tgcggacccg ttcgccgatg
     2641 cggcggatcg ccgtcacggg agtcccaagc cgcaccgccg gcttgacctg ctcgtagaga
     2701 gcgccggcga tccggtccat tcccccgacc ggctggaaca tcggcgcctg ctggtcgaat
     2761 atctcttcga acaagagcgg caggacccag ccccgctcct tcatgatctc ctcgagcttc
     2821 atcggcggca ccgtcgtccc cgcttcgcca taggcgccgg ggagcacgga atagccggag
     2881 cggccgtcgc ccttatagtc gccattgggg cgcgaaaggc cgccataggc gccgaggaaa
     2941 ttgaccatca cgctcttgtc gaccgccgtg agctcctcgt cgagcgcgcc tttcctgaca
     3001 gccttggaaa gcaattcggc gactcgcccg cgcacgtcgt tgacggcctg ccggttggcg
     3061 acgttgcggc cgccgaaatc ccatttcgcc gcgcggttcg aattgaccat cacctcgagc
     3121 ggaacgccga gccgcttggc atagccgagg atgacgtgat gcgcggtggg aatccgggcc
     3181 gcgccggcgt tgagatagag gcccttggaa aagccgcaga cctggtccgg ccggcccatc
     3241 tgctcgatcc ggtcgccgcc gcgcaccgtc cagacccggc cgccgacccg gtcgcgcgct
     3301 tcgaggacgg tgacgtccca gcccgcgcgg cgaagctcgt aggccgcgac cagcccggcg
     3361 attccggcgc ccaggacgac aaccgaacct ttccctcgcg gcgccggcgg cagagcgaaa
     3421 ggctcggcgg cggccggcgg agcgttgagg agccccatcg cctccattcc gagatagacg
     3481 gcgctcacgc ccccggcgag gccgaggcgc tcgagcaggg cgcgccgggt gaggctcacc
     3541 ggcctttcgc cttcgcggcg gtcgcttcga tctcgaccag ccagcctgga tcgaccagcc
     3601 ccgccgcctg aagcgtcgat cgcgccggct tgccgggctg cgccgcggtt ccgaaaaact
     3661 cgcgataggc cgtcatcatc ccggcgaaat ccatccgcgc ctgccccggc ggagcgatca
     3721 ggaagacccg catcatgacg atatcgccga ggcccatgtc gtgctgcttc aactgcgcct
     3781 cgatctttcg aagcacgctt cgggtctggg c
//
LOCUS       DASWNU010000336         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_195677, whole genome shotgun sequence.
ACCESSION   DASWNU010000336 DASWNU010000000
VERSION     DASWNU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195677"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VGB57_06715"
     CDS             <1..1350
                     /locus_tag="VGB57_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEY0131081.1"
                     /translation="LLPAPGGMSPAAWRTTAIIVLMAAWWMTEALPLTATALVPFLAF
                     PMLGIMTATESAASYYSPILFLVLGGAIVALAIERTGLHRRLALAIVSRGGGGPAQML
                     FAFMAATALLSMIVSNTATTLIMIPIGLAILRAARIEPGHTDGFAGALVMGIAFAATI
                     GGFGTLVGSPTNAIAAGIIEKATGYRIDFLTWALYGIPLVLFCVPLCWAILMKVQRVS
                     ATDFDPAAARAGIGETGLWSTAEKRLVPLVALTVAAWIAVPFVTPYLPKDSLTDGTIA
                     IIAALLLFVIPDGTGRAILNWEEANRAPWGVIMMFGGGLALAAGIGASGLAEWMGVAL
                     QPLRSVHPLIVAAVLVGIVIVVTEFASNVATASGAIPVVAGIIAATGVDPILLALPAA
                     FASSWGFMLPSGTGPNAIAWATGHIALPRMLKAGFLLDLAGVPIMVGIVWGIGAMLN"
     gene            complement(1442..2275)
                     /locus_tag="VGB57_06720"
     CDS             complement(1442..2275)
                     /locus_tag="VGB57_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0131082.1"
                     /translation="MTETAIANPGQSAKWNAGTGRAWAELGDMLDRLLEPFVPLVLDS
                     IEPIAGRRILDVGCGAGALSLAAAGRGAEALGVDISAPLVETALTRAGRIGASAYFVQ
                     ADAQSHRFEPHRFDALVSRFGVMFFADPVEAFRNLRSAVRPGGALACLAWRDPAENGF
                     MTAAERAAADVLPELPARIEGAPGQFGFADSGRVGAILTESGWLDVAVRPVDVECRLP
                     EEDLRLYARRMGPVADLLPTLDEATRAEVERRVDVAFDPYLENGEARFTAACWLVTAS
                     A"
     gene            2725..>3974
                     /locus_tag="VGB57_06725"
     CDS             2725..>3974
                     /locus_tag="VGB57_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEY0131083.1"
                     /translation="MQDENDITGATPRRRPKASALEIGGRRLKPSTLMMGYGFDPTLS
                     EGSLKPPIFLTSTFVFENAAAGKRFFEGVTGKRPGGAEGLVYSRFNGPNQEILEDRLS
                     LWEDAEDALTFSSGMSAIATLLLTFVQPGDVIVHSGPLYAATETLIARILGRFGVKWL
                     DFPAGATREEIESVLERAKAMGRVSLIYLESPANPTNALVDVEAVAAARSAVLGDERP
                     PIAIDNTFLGPLWAQPLRQGADLSVYSLTKYAGGHSDLVAGGITGSKELINTIRMLRN
                     TIGTITDPNTAWMLLRSLETLELRMIRAGENADKVCRFLRDHPKVESVGYLGFLEDGG
                     DARQADIYRRHCTGAGSTFSLYLKGGERESFAFLDSLKIAHLAVSLGGTETLASAPAA
                     MTHLSVPDERKQALGITDNLVRIS"
BASE COUNT          582 a         1476 c         1282 g          634 t
ORIGIN      
        1 ctgctcccgg ccccgggcgg catgtccccg gccgcctggc gaaccacggc gatcatcgtc
       61 ctgatggcgg cctggtggat gaccgaggcg cttccgctca ccgcgaccgc tttggtcccg
      121 ttcctcgcct tcccgatgct cgggatcatg accgccaccg aatccgcggc ctcttattat
      181 tccccgatcc tgttcctcgt cctcggaggc gccatcgtcg ccctcgcgat cgagcggacc
      241 ggcctccacc gccgcctcgc tctggccatc gtcagccgcg gcggcggcgg gccggcgcag
      301 atgctgttcg ccttcatggc cgcgaccgcc ttgctcagca tgatcgtctc caacaccgcc
      361 accaccctca tcatgatccc gatcggcctc gccatcctcc gcgcggcccg gatcgagccc
      421 ggccacaccg acggcttcgc cggggcgctc gtcatgggca tcgccttcgc ggcgacgatc
      481 ggcggcttcg gcaccctcgt cggctccccg accaacgcca tcgccgcggg catcatcgag
      541 aaggcgaccg gctaccggat cgatttcctc acctgggcgc tctacggaat ccccctggtc
      601 ctcttctgcg tgcccctgtg ctgggcgatc ctgatgaagg tgcagagagt ctcggccacc
      661 gacttcgacc ccgccgccgc ccgtgccgga atcggcgaga ccggcctctg gtcgaccgcc
      721 gagaagcgcc tcgtcccgct cgtcgcactc accgttgcgg cgtggatcgc ggtcccgttc
      781 gtaacgccct atttgcccaa ggattcgctc accgacggca ccatcgccat tatcgccgcc
      841 ttgctcctct tcgtcatccc cgacggcacc gggcgcgcca tcctcaactg ggaggaggcg
      901 aaccgggcgc cctggggagt catcatgatg ttcggcggag gcctcgccct ggccgccggg
      961 atcggcgctt cgggcctcgc ggaatggatg ggcgtggcgc ttcagccgtt gcgctcggtc
     1021 catcccctga tcgtcgcggc ggtgctcgtc gggatcgtca tcgtcgtcac cgaattcgcc
     1081 tcgaacgtcg cgacggcgag cggcgccatt ccggtcgtcg ccgggatcat cgccgccacc
     1141 ggcgtcgatc ccatccttct cgcactcccc gccgccttcg cctcgagctg gggcttcatg
     1201 ctcccgtccg gaaccggccc caacgcgatc gcctgggcga ccggccacat cgccctgccg
     1261 cgaatgctca aggccggctt cctgctcgac ctcgccgggg tgccgatcat ggtcgggatc
     1321 gtctggggga tcggggcgat gctgaattaa cccgtcatcc cggcgaaagc cgggacctcg
     1381 ggtcggagag gctcggcggg tctccccgag gtcccggcct tcgccgggat gacggcttcg
     1441 ttcaggcgct ggccgtcacc agccagcagg ccgccgtgaa gcgcgcctcg ccgttttcca
     1501 gatagggatc gaaagccaca tcgacgcgcc gctcgacctc ggcgcgagtc gcctcgtcca
     1561 gggtcgggag caggtcggcg accggcccca tccgccgcgc gtagaggcgc agatcctcct
     1621 cgggcagccg gcactcgacg tcgacgggcc ggacggcgac gtcgagccag ccgctctccg
     1681 tcaggatcgc gcccacccgg ccggaatccg cgaagccgaa ctggccggga gcgccttcga
     1741 tccgcgccgg cagctccggc aggacgtccg ccgccgcgcg ttcggccgcg gtcatgaagc
     1801 cattctccgc cggatcgcgc caggcgaggc aggcgagagc gccgcccggc cggacggcgg
     1861 agcgcagatt gcggaaagcc tccaccggat cggcgaagaa catcactccg aagcgcgaga
     1921 ccagagcgtc gaaccgatgc ggctcgaacc ggtgggactg ggcgtcggcc tggacgaaat
     1981 aggccgaggc gccgatccgg cctgcccgag tgagcgccgt ctcgaccagc ggagccgaga
     2041 tgtcgacccc gagcgcctcc gccccccggc ccgccgcggc gagcgagagg gccccggcgc
     2101 cgcagccgac gtcgaggatt cggcgcccgg cgatcggttc gatcgagtcg aggacgagcg
     2161 ggacgaacgg ctccagcagc cggtcgagca tgtcgccgag ctcggcccag gcgcggcccg
     2221 tcccggcgtt ccatttagcg gactggcccg gattggcgat tgcggtctcc gtcatcgtgg
     2281 cattttcccc catttgcgcc ggagcaggct agccggttcg ccgggccccg tcattcccgg
     2341 gaggcggatg cgtcggatcc gtcatcccgg cgaaggccgg gatccatgaa cacggatcga
     2401 agcatttcct ccaccggccg tgtccatgga ttccggcctt cgccggaatg acaggcggtc
     2461 ggcggattgc atagccgtca acccgaaagc cgggacctcg ggacgagaag tctcggcggg
     2521 tctccccgag gtcccggcct tcgccgggat gacggggcgt gagtcgcacg cgacgcgccg
     2581 ccgcatcggg gctagcggcg cggccgcctg ttgggttagg gagccggcgg gcaaccgacc
     2641 tcaccccccg gggccgcgct tccaaggcgc ggccggtgag cctgacctaa ggcaggctcg
     2701 gtctccacga caggaggcat gttcatgcag gacgaaaacg acatcaccgg cgccactccc
     2761 cggcgccgtc ccaaggcgag cgctctcgaa atcggcggcc gccgcctcaa gccgtccacc
     2821 ctgatgatgg gctacggctt cgatccgacg ctttcggaag gctcgctgaa gccgcccatc
     2881 ttcctcacct cgaccttcgt cttcgagaat gccgccgctg ggaagcgctt cttcgagggt
     2941 gttaccggca agcgcccggg cggcgccgag ggcctcgtct attcgcgctt caacggcccc
     3001 aatcaggaga tactcgagga ccggctcagc ctgtgggagg atgccgagga cgcgctgacc
     3061 ttctcctccg ggatgtcggc gatcgcgacc ttgctgctca ccttcgtcca gcccggcgac
     3121 gtcatcgtcc actccggtcc gctctatgcc gcgaccgaga ccctgatcgc ccgcatcctc
     3181 ggccgattcg gggtcaagtg gctcgatttc ccggccggcg cgacccgcga ggagatcgaa
     3241 tcggtgctgg agcgcgccaa ggcgatgggc cgggtctcgc tcatctatct tgaaagcccg
     3301 gccaatccga ccaacgcttt ggtcgacgtc gaggccgtcg ccgcggcgcg ctcggccgtg
     3361 ctcggcgacg aacggccgcc gatcgcgatc gacaacacct tcctcggccc gctctgggcg
     3421 cagccgctcc gccagggcgc cgacctgtcc gtctactcgc tgaccaaata tgccggcggc
     3481 cactcggatc tcgtcgccgg cgggatcacc ggctctaagg agctgatcaa cacgatccgg
     3541 atgctccgca acacgatcgg gacgatcacc gatcccaaca ccgcctggat gctgctcagg
     3601 agcctcgaga cgctcgagct ccggatgatc cgggcgggcg agaatgcgga caaggtgtgc
     3661 cgcttccttc gcgaccatcc caaggtcgag agcgtcggct atctcggctt cctcgaggac
     3721 ggcggcgacg cccgccaggc cgacatctac cgccgccact gcaccggcgc cggctcgacc
     3781 ttctcgctct atctgaaggg cggcgagcgc gaatccttcg ccttcctcga ttccctcaag
     3841 atcgcccacc tcgccgtcag cctcggcgga accgagactt tggcgagtgc ccccgcggcg
     3901 atgacccatc tgtcggtgcc ggacgaacgc aagcaggcgc tggggatcac ggataatctg
     3961 gtgcggatct cgat
//
LOCUS       DASWNU010000337         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_141836, whole genome shotgun sequence.
ACCESSION   DASWNU010000337 DASWNU010000000
VERSION     DASWNU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141836"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /gene="cpaB"
                     /locus_tag="VGB57_06730"
     CDS             complement(<1..498)
                     /gene="cpaB"
                     /locus_tag="VGB57_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0131084.1"
                     /translation="MDVKKLVLLIGALIVAAVTAVTAKNMFSGAGAPSASAKPAVPAG
                     PEVLVATRSLPVGTIIDAEAFRFQRWPEGLVQPAYYIKGKPGVRPADLVGTVVRNEIT
                     AGQPFTQGSLIRPGDRGFLAAALGPGMRAVTVAVSATSGVAGFVFPGDRVDIVLTQEI
                     EGGGDG"
     gene            complement(539..1009)
                     /locus_tag="VGB57_06735"
     CDS             complement(539..1009)
                     /locus_tag="VGB57_06735"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0131085.1"
                     /translation="MQGALSWFFIALLAAAMLWAAIGDWRTRTIPNWLNGAIALMAIP
                     FWWSIGLGLWPGVALQIGLALAVFGLFAIAFRFGAMGGGDVKMAAALALWLPFAGVVK
                     LLAVMSIAGGVLTVAMLAAHRMAKAAGQPEIPYGIAIAFGGFWVLGERFLNQFV"
     gene            1058..1996
                     /locus_tag="VGB57_06740"
     CDS             1058..1996
                     /locus_tag="VGB57_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131086.1"
                     /translation="MHSEAFDRRAIPSDARFGEWKAADGWTVRTLDRRQKPGAEVRGS
                     LLFAGGRGDFIEKYLEAQDHWHRRGWNVTAFDWRGQGASQGDLPGGHLDSFDTLVGDL
                     AGLIEQWRGETARPHVVVAHSMGGHVLLRALADRRAKIDAAVLVAPMLMINSGPMPPV
                     AAQWLASTASLFGWSGQPAWQQPSRPEPAGSMRQAFLTGCPDRYDDELWWWEKHPSFN
                     LGAPSWGWLKAAYQSCAALTPASLAGVETPVLLIGTERDRLVSPAAIRRAASLLPRAE
                     LVMVEDAAHEILRERDEVRLRALGSIDAFLDRSAGR"
     gene            1993..>2465
                     /locus_tag="VGB57_06745"
     CDS             1993..>2465
                     /locus_tag="VGB57_06745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0131087.1"
                     /translation="MSFDADIAVVGAGMAGASLAAEVAAEASVILIEAEDQPGYHSTG
                     RSAAFWAESYGGPFVQPLTTQSGPFLGEQGFLKARGAIHVADRDGLEALAAVETEFGD
                     RVAFERLDRPALEARLPGLRGGWEAGLIEPSCADIDVAGLHAFYLARARRRGAKLA"
BASE COUNT          389 a          872 c          858 g          346 t
ORIGIN      
        1 tccgtcgccg ccgccttcga tctcctgggt gaggacgatg tcgacccggt cgcccgggaa
       61 gacgaagccg gcgactccgc tggtcgcgga gacggcgacg gtgaccgccc tcatccccgg
      121 cccgagcgcg gcggcgagga agccgcggtc gcccggcctg atcagcgagc cctgggtgaa
      181 gggctggccg gccgtgatct cgttgcggac gaccgttccg accaggtcgg ccgggcgaac
      241 cccgggcttg cccttgatgt agtaagcggg ctggacgagg ccttcgggcc aacgctggaa
      301 gcggaaggcc tcggcgtcga tgatggttcc gacgggcagc gagcgggtcg ccaccagcac
      361 ttccgggccg gccgggaccg cgggcttggc ggaggccgaa ggcgctccgg ctccgctgaa
      421 catgttcttc gcggtcaccg cggtaaccgc cgcgacgatg agcgcgccga tcagcagcac
      481 gagctttttc acatccatga cgccagtcgc ctcctcgccc tcatatgggg cctacacatc
      541 agacaaattg gttaagaaac cgttcgccaa gcacccaaaa accaccgaat gcgatggcga
      601 tgccgtacgg aatttcgggc tggccggcgg ctttcgccat ccggtgcgcg gccagcatcg
      661 caacggtcag cacgccgccc gcgatcgaca tgaccgcgag aagcttcacg accccggcga
      721 agggcagcca caaagcgagc gccgcggcca tcttgacgtc gccgccgccc atcgctccga
      781 agcggaaggc gatcgcgaac agcccgaaga ccgccagggc aagcccgatc tgcagggcga
      841 cgcccggcca caggccgagg ccgatgctcc accagaaggg tatggccatc agggcgatcg
      901 ctccgttcag ccagttgggg atcgtcctgg tccgccagtc gccgatcgcc gcccacagca
      961 tggccgcggc gagcaaagcg atgaaaaacc aggaaagagc cccttgcatg gggaccaggc
     1021 actagacgta acagctttcg aaaccgtaac cggccccatg cacagtgaag cgttcgaccg
     1081 ccgcgccatc ccgtccgacg cccgcttcgg cgagtggaag gctgccgacg gctggacggt
     1141 ccgaaccctg gatcggcggc agaagccggg ggccgaggtc cgcggcagcc tcctgttcgc
     1201 gggcggccgg ggcgacttca tcgagaaata tctcgaggcg caggaccatt ggcaccgccg
     1261 cggctggaac gtcaccgcct tcgactggcg agggcagggg gcctcacagg gcgaccttcc
     1321 cggaggccat ctcgacagct tcgacaccct ggtcggagat ctcgctgggc tgatcgagca
     1381 gtggcgcggc gagaccgccc ggcctcatgt cgtcgtcgcc cattcgatgg gcgggcacgt
     1441 cctcctgcgc gccctcgccg accggcgcgc gaagatcgac gccgcggtgc tggtcgctcc
     1501 gatgctgatg atcaacagcg ggccgatgcc gcctgtggcc gcccagtggc tcgcctcgac
     1561 cgcctcgctg ttcggctgga gcgggcaacc ggcctggcag cagccctcgc ggccggaacc
     1621 cgccggctcg atgcgccagg ccttcctgac cggctgcccc gaccgctatg acgacgagct
     1681 gtggtggtgg gagaagcatc cgagcttcaa cctcggcgct ccgagctggg gctggctgaa
     1741 ggctgcctac caatcgtgcg cggccctgac tcccgccagc ctcgccggag tggagactcc
     1801 ggtcctgctg atcggcaccg agcgcgaccg gctcgtaagc ccggcggcga tccggcgcgc
     1861 cgcctcgctt ctgccccggg cggagctcgt catggtcgag gatgccgcac acgagatcct
     1921 gcgcgagcgc gacgaggtcc gccttagagc gctgggctcg atcgacgcct tcctcgaccg
     1981 aagcgcgggc cgatgagctt cgacgcggac atcgccgtcg tcggcgccgg aatggcgggg
     2041 gcgagccttg ccgcggaggt cgccgccgag gcctccgtca ttctgatcga ggccgaggac
     2101 cagccaggct atcattccac gggccgctcg gccgccttct gggcggaaag ctatggcggc
     2161 cccttcgtcc agccgctcac cacgcaatcg gggcccttcc tgggggagca gggcttcctg
     2221 aaggcgcgtg gggcgatcca cgtggccgac cgcgacgggc tcgaggcgct ggcggcggtc
     2281 gagaccgagt tcggagaccg ggtcgccttc gaacggctcg accggccggc gctcgaagcc
     2341 cggctgcccg ggcttcgcgg cggctgggag gccggcctga tcgagccgag ctgcgcggat
     2401 atcgacgtcg ccggcctgca cgctttctac ctcgcccggg cccgccgccg gggcgcgaag
     2461 ctggc
//
LOCUS       DASWNU010000338         6070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_38895, whole genome shotgun sequence.
ACCESSION   DASWNU010000338 DASWNU010000000
VERSION     DASWNU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38895"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGB57_06750"
     CDS             <1..430
                     /locus_tag="VGB57_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEY0131088.1"
                     /translation="SQASTDPLNGIINPKLIPGARLIYTVTVLNPNAFAATADSIVIV
                     DPTPARLSLFVNNISGVSGGPLRFQNGATLSGLSYTFTSLASLTDDIDFSSDGGATWT
                     YVPAPDSQGGDAAVNRIRIRPKGAMAANSSFSLLFGYVIK"
     gene            complement(435..519)
                     /locus_tag="VGB57_06755"
     tRNA            complement(435..519)
                     /locus_tag="VGB57_06755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Leu,seq:caa)
     gene            complement(587..1114)
                     /locus_tag="VGB57_06760"
     CDS             complement(587..1114)
                     /locus_tag="VGB57_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY0131089.1"
                     /translation="MHWMFLPLKRYAEFSGRSRRTEYWMFFLFQILLAVAFWVLMAIV
                     GGGALMSGGDATALAAAGGAIMIVSALYGLVSLALLIPAIAVTVRRLHDTNRTGWWVL
                     APIAGYVLVLVGVAMAASSPDNPGLGGILAMVGGIAALVLALVLLVFMFLEGTRGPNN
                     YGPDPKGEALDKVFA"
     gene            complement(1179..1739)
                     /locus_tag="VGB57_06765"
     CDS             complement(1179..1739)
                     /locus_tag="VGB57_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131090.1"
                     /translation="MQTVVAELEVAFAVKEGWMNVAGQIFVALLSLSDAAAVPAPAGW
                     GPGPSGQFRFSAGGAPVYLLECKATEVVVTQFGVTQLVDLQANRPVGDTEGTALPPGA
                     ALMALATDRTDEPNMMPAAAVRNPDKGWDMTIRLAKDDPALLSLPRAKFISLFTTGFT
                     RAAKLSKGEQKLLAAFMNRCRGKIPG"
     gene            complement(1747..3255)
                     /locus_tag="VGB57_06770"
     CDS             complement(1747..3255)
                     /locus_tag="VGB57_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY0131091.1"
                     /translation="MVAHVATVAFLGLEARAVEVQVQISAGLPKFLVVGLPDKAVAES
                     RERVHAALAAIGLSLPPKRITVNLSPADLPKEGSHYDLPIALGMLAALGAIDTETLAG
                     YVAVGELGLDGRIQPSPGVLLAAMNASARSLGLVCPAAQGSEAAWAGDVEIVAAPDLI
                     ALLNHFRGSAILSPPAPGEAEPSGFGPDLRQVKGQETAKRALEITAAGGHNLLMVGPP
                     GAGKSLLAACLPGILPELTPSEALEVSMIASVAGSLSGGRLIRTRPYRAPHHSASMVS
                     LVGGGLRVRPGEVSLAHLGILFLDELPEFQRSALDSLRQPLETGTVSVARANAHVTFP
                     ARVQLVAAMNPCRCGHLGDPALACSRAPRCAADYQARVSGPLLDRIDLHVDVQAVSAA
                     DLVLPPPSEGSAEVARRVGGARAVQTARYEGEGIRTNAEVDGERLEAVARPDEPGRRL
                     LAQAAEAMRLSARGFHRVMRVARTVADLAGSEEVRRIHVAEALSYRRQAPRS"
     gene            3428..3658
                     /locus_tag="VGB57_06775"
     CDS             3428..3658
                     /locus_tag="VGB57_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131092.1"
                     /translation="MVTTEAANGHRNFYMVNVDDADRARDAVNRMMGMNTAQALAPIP
                     DETIAQHNLTPGQAWLCTTTNAVGEVTHSELK"
     gene            4120..4254
                     /gene="rpmH"
                     /locus_tag="VGB57_06780"
     CDS             4120..4254
                     /gene="rpmH"
                     /locus_tag="VGB57_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY0131093.1"
                     /translation="MKRTFQPSNLVRKRRHGFRARTATVGGRKVLAARRARGRKTLSA
                     "
     gene            4251..4568
                     /gene="rnpA"
                     /locus_tag="VGB57_06785"
                     /pseudo
     CDS             4251..4568
                     /gene="rnpA"
                     /locus_tag="VGB57_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
     gene            4673..4885
                     /gene="yidD"
                     /locus_tag="VGB57_06790"
     CDS             4673..4885
                     /gene="yidD"
                     /locus_tag="VGB57_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY0131094.1"
                     /translation="MIAKLFIWVARSWQLGPSRVLPPSCRFQPSCSAYAITALQRYGA
                     LRGGWMAARRLLRCHPWGGSGHDPVP"
     gene            4896..>6070
                     /gene="yidC"
                     /locus_tag="VGB57_06795"
     CDS             4896..>6070
                     /gene="yidC"
                     /locus_tag="VGB57_06795"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY0131095.1"
                     /translation="MNDHRNMILAIVLSAIVLIGWSFLSEQFFPTAAPPTTKVVDGKS
                     VPIPQPKADPAADGPVAIRDRNQVLRETPRIRIETPHLAGSISLKGARIDDLVLTTHR
                     EGVAKDSPPIRLLSPEGSPGAYFASLGWIGQPQRVPGPETLWTSTSTRLAPGSPAILN
                     WDNGQGQLFQIVLAVDEGYLFSVDQRVVNRGAGDFAARPYSVLSRRGASKDPDLWTAH
                     VGPMGVFNGAAEYGNDYSTIAEAGERRFNSRGGWIGFTDTFWLAALVPDQGKAVESTF
                     RHKSAGDSYQADAIAEPIMTPRGTQSRYTSHVFAGAKEIELLDRYTEQLGTPLERAID
                     WGWFRWFMQPIFSLLNWLYAVLGNFGLAIICLTLIVRGLMYPIAQKQFKSMAAMRVL"
BASE COUNT         1046 a         2091 c         2012 g          921 t
ORIGIN      
        1 gagccaggcg agcacggatc ccttgaacgg aatcatcaat cccaagctga tcccgggtgc
       61 gaggctgatc tatacggtga cggtcctgaa ccccaacgcc ttcgcggcca ccgccgacag
      121 catcgtcatc gtcgatccca ctccggcgag gctctcgctc ttcgtgaaca atatctcggg
      181 cgtgagcggc ggcccgcttc gattccagaa cggcgccacg ctctccgggc tcagctacac
      241 cttcacgagc ctggcgagcc tgaccgacga catcgacttc tccagcgacg gcggcgcaac
      301 ctggacctac gtgccggcgc ccgacagcca gggcggcgac gccgccgtga accgaatccg
      361 aatccgcccc aaaggcgcga tggccgccaa ttcgagcttc agcctgctct tcggctacgt
      421 catcaaataa ggaatggtgc ccccggccgg agtcgaaccg gcactccttg cggaactcga
      481 ttttgagtcg agcgcgtcta ccaattccgc cacgggggca cgcgggccac gccctagcca
      541 agcgcggggc gggccgcaac cgcccgcccg cgcccggcag acagggtcag gcgaagacct
      601 tgtcgagcgc ctcgcctttc gggtccggtc cgtaattgtt cggcccgcga gtcccttcga
      661 ggaacatgaa gacgagcagg acgagggcca ggacaagcgc cgcgatgccg ccgaccatgg
      721 ccaggatgcc gccgagaccg ggattgtcgg gcgagctcgc cgccatcgcg acgccgacca
      781 gcaccagcac atagccggcg atcggcgcaa gcacccacca gccggtccgg ttggtgtcgt
      841 gcagcctgcg gaccgtgacg gcgatggcgg gtatgagcaa ggccagcgag accagcccgt
      901 acaaagcgga gacgatcatg atggccccgc ctgcagccgc cagcgcggtc gcatcgccgc
      961 cactcatgag agcgccgccg cccacgatgg ccatgaggac ccagaaagcg acggccagca
     1021 ggatctggaa aaggaagaac atccaatatt cggtccgccg cgagcgacct gaaaattcgg
     1081 catagcgctt cagcggcaaa aacatccaat gcatagcaat cccccttttg ccgcatcatt
     1141 gcggtccggg aacatcttcc ggatttagag gggcggtttc aacccggaat cttgccccga
     1201 caccggttca tgaatgcggc gagaagcttc tgctcgccct tgctcagctt ggccgcgcgc
     1261 gtgaaacctg tcgtgaacag agaaatgaat ttcgcgcggg gcaggctcag caaggcagga
     1321 tcatccttgg cgagcctgat cgtcatgtcc caacctttgt ccggattcct cacagcagct
     1381 gccggcatca tgttcggctc gtccgtcctg tcggtggcga gcgccatcaa ggccgctccg
     1441 ggcggcaagg ccgttccctc cgtgtctccg acgggccgat tggcctgcag atcgacgagc
     1501 tgcgtcactc cgaactgggt gacgaccacc tccgtcgcct tgcattccag caggtagacg
     1561 ggcgctccgc ctgcgctgaa cctgaactgc ccggaaggcc ccgggcccca gcccgcgggt
     1621 gccggtaccg ccgctgcatc cgacaacgac agcagcgcga cgaatatctg tccagccacg
     1681 ttcatccatc cctccttgac tgcgaaagcc acctcaagct cggcgacgac cgtttgcatc
     1741 cagcgctcaa cttcgcgggg cctgccgtcg atagctgagc gcctcggcga catggatccg
     1801 gcggacctcc tcgctccccg ccaggtccgc caccgtgcgc gccacccgca tcacccggtg
     1861 gaagccgcgc gccgagaggc gcatggcctc ggcggcctgg gcgaggagtc ggcggcccgg
     1921 ctcgtccggc ctcgccaccg cctcgagccg ttcgccgtcg acttccgcgt tggtgcggat
     1981 gccctctcct tcgtagcggg cggtctggac ggcgcgcgca ccgccgaccc ggcgggcgac
     2041 ctcggcggag ccttccgaag gcggcggaag gacgagatcg gccgcggaga ccgcctggac
     2101 atcgacatga aggtcgatcc ggtcgagcaa cgggcccgag acccgcgcct gatagtcggc
     2161 ggcgcagcgc ggcgcccgcg agcaggccag agcggggtcg ccgagatggc cgcagcggca
     2221 cgggttcatc gccgcgacga gctgcacccg ggcgggaaag gtgacgtggg cattggctcg
     2281 ggcgacgctg accgtgccgg tctcgagcgg ctggcgaagc gaatcgaggg cggagcgctg
     2341 gaattcggga agctcgtcga ggaacaggat tccgagatgg gcgaggctga cctctccggg
     2401 acggacgcgc aggcctccgc cgaccagcga caccatcgag gcgctgtgat ggggcgcgcg
     2461 gtagggccgc gtccggatca gcctgccgcc cgagagggag ccggcgacgc tcgcgatcat
     2521 cgacacttcg agcgcctcgg acggagtcag ctccggaagg atcccgggca ggcaggcggc
     2581 aagcagcgac ttgcccgccc cgggcggacc gaccatcagc aggttgtggc cgccggcggc
     2641 tgtgatttcg agagcccgct tggcggtctc ctggcccttc acctgtctga gatcgggacc
     2701 gaagcccgac ggctcagcct cgccgggcgc ggggggcgag aggattgcgc tgccccggaa
     2761 atggttgagc agggcgatga ggtcgggcgc cgcgacgatc tcgacgtcgc cggcccaggc
     2821 cgcttccgag ccttgcgccg ccgggcagac gaggccgagg cttcgcgccg aggcgttcat
     2881 cgccgccagc aatacccccg gcgacggctg gatccggccg tcgagcccga gttcgcccac
     2941 cgcgacgtag ccggccaggg tctcggtatc gatcgccccc agagcggcga gcattccgag
     3001 cgcgatcgga agatcgtaat gggagccctc cttgggcaga tcggcgggcg agaggttcac
     3061 cgtgatccgc ttgggcggaa gcgagagccc gatcgcggcc aaagcggcgt ggacccgctc
     3121 tcggctttcg gccaccgcct tgtcgggaag gccgacgacg aggaatttgg gcaggccggc
     3181 cgaaatctgg acctgtacct cgaccgcccg cgcctccagc cccaggaacg ccaccgtggc
     3241 gacatgcgcg accattatgc caagccctcc gtctacgcct tttcggtgga cagatgcacc
     3301 ttagtcaacg ctttccgtaa ctcacccgga ctgcagattt gacggcgcct gcaaacaggc
     3361 cgatcagtag cgaagcggac ctatcgggag ccgcgcaata gaagcggagg gatggatgag
     3421 cggatatatg gtgacaaccg aagctgccaa cggtcacaga aacttctaca tggtgaacgt
     3481 cgacgacgcg gaccgggcgc gcgacgcggt caaccggatg atgggcatga acaccgccca
     3541 ggcgcttgct cccattcccg atgaaaccat cgcccagcac aatctcaccc cgggccaggc
     3601 gtggctctgc accaccacca atgcggtcgg cgaggttacc cacagcgagc tcaagtgatc
     3661 gcggcccaag ccgtcttgcg cggctaacac agcttcccca catgggggcg atgggcagga
     3721 tcgattggcg gcacctgacg aactatccgg cggtgcgaag cgcactggtc gtggtcgggt
     3781 tcatcctgat cgggctgacg cccctgctcg ggccgcttcc gggtccgggc gggatcgtcg
     3841 tgttcgccgc cggcctctcg ctggtgctga agtacagcgc ctgggccaag cgctattatg
     3901 tccgcttcaa gcggcgccac ccgaagaagg gcgactgggc cgactggggc ctgaggcggg
     3961 ccagcgccaa gcgccggcag gagcggctca aggccgagca ggaggccgtg gcggccatgg
     4021 ggcaattgac ttgcggggag gatttgccta acggggccgg caacgaaggc cgctccgcga
     4081 tgggcggccc ttttaattcc agttttcaag gattgagcga tgaagcgcac cttccagccg
     4141 agcaatctcg tgcgcaagcg ccggcacggc ttccgggccc ggacggcgac cgtcggcggc
     4201 cgcaaggttc tggccgcgcg ccgtgcgcgg ggccgcaaga ccctctccgc ctgagctccc
     4261 tcacccgccg agccgatttc ctggccgcca accgcggccg gcgcgcgccc atgcccggtt
     4321 tcgtccttct ggtgaggccg cgcgaggacg gcgacacggg gatccggctg gggatcacgg
     4381 tcacgaagaa gatcggcggc gcggtggtgc gcaaccggat gaagcggcgc ttccgaagcc
     4441 tcgcccgcga ccttctgccg gagaacggta tcgccggcgc cgaccatgtg ctgatcggcc
     4501 gccagggcgg gatcgagcgg gatttcgggc tgctgagggc ggagctggag aaggcgctgc
     4561 aaaagttggg acgaaacccc gtcatcccgg cgaaagccgg gacctcggga caagggggct
     4621 cggcgggtct ccccgagatc ccggcttccg ccgggatgac gaagcgctcg aagtgatcgc
     4681 gaagctgttc atctgggtcg cccgtagctg gcaattgggc ccttcgcggg tgcttccgcc
     4741 gagctgccgc ttccagccca gttgctcggc ctatgcaatc accgcgctcc agcgctatgg
     4801 ggcgcttcgc ggcggctgga tggccgccag acgtctgctc agatgccacc cttggggggg
     4861 ctcgggccat gatccggtac cgtgaggggg aataggtgaa cgaccaccgt aacatgatcc
     4921 tggcgatcgt cctgtcggcg atcgtcctga tcggctggag ctttctcagc gagcaattct
     4981 tccccacggc cgccccgccg acgaccaagg tggtcgacgg caagtcggtg ccgattcccc
     5041 agcccaaggc ggatcccgcg gccgacggcc ccgtcgctat ccgggaccgc aaccaggtgc
     5101 tgcgcgagac gccgcggatc cggatcgaga ctccgcacct cgcgggctcg atcagcctca
     5161 agggcgcacg gatcgacgat ctcgtcctca ccacccatcg cgaaggcgtg gccaaggact
     5221 ccccgccgat ccgcctgctc tcccccgaag ggagtcccgg ggcctatttc gccagcctcg
     5281 gctggatcgg ccagccgcag cgcgtgccgg ggccggaaac gctttggacc tcgacctcga
     5341 cccggcttgc gccgggcagc cccgcgatcc tcaattggga caacggccag ggtcagctgt
     5401 tccagatcgt cctcgccgtc gacgagggct atttgttctc ggtcgatcag agagtcgtca
     5461 atcgcggcgc cggcgacttc gcggcgcggc cttattccgt gctcagccgg aggggcgcct
     5521 ccaaggatcc cgatctgtgg acggcccatg tcggtccgat gggagtcttc aacggcgccg
     5581 ccgagtacgg caacgactac agcacgatcg ccgaagcggg cgaacgccgc ttcaacagcc
     5641 gcggcggctg gatcggcttc accgacacct tctggctggc cgccctcgtc cccgatcagg
     5701 gcaaggccgt cgaatcgacc ttccgccaca agagcgccgg cgacagctac caggccgatg
     5761 cgatcgccga gccgatcatg accccgcgcg ggacccagtc ccgctacacc tcccacgtct
     5821 tcgccggggc gaaggagatc gagctgctcg accgctatac ggagcagctc ggcaccccgc
     5881 tcgaacgggc gatcgactgg ggctggttcc gctggttcat gcagccgatc ttctcgctcc
     5941 tgaactggct ctacgcggtg ctcggcaatt tcggcctggc gatcatctgc ctgaccctga
     6001 tcgtgcgcgg cctcatgtac ccgatcgccc agaagcagtt caaatcgatg gcggcgatgc
     6061 gcgtgctcca
//
LOCUS       DASWNU010000339         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_246512, whole genome shotgun sequence.
ACCESSION   DASWNU010000339 DASWNU010000000
VERSION     DASWNU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246512"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..>2693
                     /locus_tag="VGB57_06800"
     CDS             334..>2693
                     /locus_tag="VGB57_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0131096.1"
                     /translation="MRRLLLPFALFSAFAVTACASTTSDEVASASDAPAAEAAPEAAP
                     EAAGGPVPVAQLVRQIDIPYEQFTLDNGLRVVVHEDRKAPVVAISIWYNVGSKDEPKG
                     RTGFAHLFEHLMFNGSENAPGDYFEPLREIGATDFNGTTWFDRTNYFQTVPKPALERG
                     LFLEADRMGHLLGAVTQQNLTNQIGVVQNEKRQGDNEPFGLVEYAQLEALFPEGHPYR
                     HSTIGSMADLDAATLEDVKNWFRSRYGPNNAVLVLAGDVSADEARPLVEKHFGSIPRG
                     PVNTPAKADVPTLSAPKVEVMKDRVANTRLYRNWIVPGMLDDAAVQLDVAAGVLGGLA
                     SSRLDNELVRKDQTAVGVRAYILPFHRVSQFEIQVDVKAGVDPDIVSARLDQIVADLI
                     RSGPTEDEVQRVVMSDVAGRIRGLEQVGGFGGKAVALAEGALYANDPEFYKKQLLAYG
                     RVTPATVRAAMQKWLTRPVYALRVEPGERKAYEEAKGSRGGAASQRPRYYRQPRPGEK
                     PMAPLPFVAPDENAPEPLSYQKIPMPEVGEISNLDFPDVERARLSNGIPVVYARRSAV
                     PVVRVAVEFDAGVAADPGDALGTQSLMLSLLEEGTTSRNSIQIAEEQERLGAAIGPET
                     SLDRTAVSLAALTPNLGPSLDLLADIVRNPAFDPGEVERLRAQQLSAIDQELTEPSGL
                     GARVLPPLLFGANHPYGRPSSGLGDKPVIARLTRDDLIRFHQSWIRPDNATIFAVGDL
                     PLAELVPQLEARFGNWSPPAAPKGTKNFAATPPAPKPRIVLVNRPQS"
BASE COUNT          438 a          965 c          888 g          402 t
ORIGIN      
        1 cgtagccgcg ggtgccggta tcgcgccctt ccagccggtc gtcggcgagg aaggcgacgt
       61 ggctccggaa gctctccggc gagaattcgg gcggagcggc ggccggctgc gccggaacgg
      121 gcgaggcgag tgcgaggctg aggagggcga cagcgaaggc gttgaagcgc atggagaatc
      181 ctgatgggct gggtttcggc cgctccctac agcagatcgg gggcgcgtca aaagcgctgt
      241 cggccccgga cgtcgagcaa tcgcggcata ttgcgccgac aaggcgcgcc gctataggtc
      301 cggacgaata agactcggga aggactgctt aaaatgcgcc gcttgttgct cccctttgcg
      361 ctcttttccg cttttgccgt caccgcatgc gcctcgacga cctccgacga ggtggcgagc
      421 gcgtcggacg ctcccgccgc cgaggccgcc cccgaagcgg cgcccgaggc cgcgggcggg
      481 cccgtcccgg tggcccagct cgtccgccag atcgacattc cctatgagca attcaccctc
      541 gacaacggcc ttcgggtcgt cgttcacgag gatcgcaagg cgccggtcgt cgccatttcc
      601 atctggtaca atgtcggctc gaaagacgag cccaagggca ggaccggctt cgcccatctg
      661 ttcgagcatc tgatgttcaa cggctccgaa aatgcgcccg gcgactattt cgagccgctt
      721 cgcgagatcg gcgcgaccga cttcaacggc acgacctggt tcgaccgtac caattatttc
      781 cagaccgtgc ccaagccggc gctcgagcgc ggcctgttcc tcgaggccga ccggatgggc
      841 catttgctcg gcgcggtgac ccagcagaac ctcaccaacc agatcggagt cgtccagaac
      901 gagaagcgcc agggcgacaa tgagcctttc ggcctcgtcg aatatgctca gctcgaggcg
      961 cttttccccg aaggccatcc ctatcgccat tcgacgatcg gctcgatggc ggacctcgac
     1021 gcggcgactc tggaggacgt gaagaactgg ttccggtcca gatacggccc gaacaacgcc
     1081 gtcctcgtcc tcgccggcga cgtctcggcc gacgaggcgc ggcctctggt cgagaaacat
     1141 ttcggaagca tcccgcgcgg cccggtcaac acgcccgcca aggccgacgt tccgaccctg
     1201 tcggcgccca aggtcgaagt gatgaaggac cgggtcgcca acacccggct ctaccgcaac
     1261 tggatcgtcc ccggcatgct cgacgacgcc gccgtccagc tcgacgtcgc cgcgggcgtg
     1321 ctcggcggcc tcgccagctc gaggctcgac aacgagctgg tgcgcaagga ccagacggcg
     1381 gtgggggtcc gggcctacat cctgcccttc caccgggtca gccagttcga gatccaggtc
     1441 gacgtcaagg ccggggtcga ccccgacatc gtctcggccc gcctcgacca gatcgtcgcc
     1501 gacctgatcc ggagcgggcc gaccgaggac gaggtccagc gggtggtcat gtccgacgtc
     1561 gccggccgga tccgggggct cgagcaggtc ggaggcttcg gcggcaaggc ggtcgccctc
     1621 gccgagggcg cgctctatgc gaacgatccc gaattctaca agaagcagct cctcgcctac
     1681 ggccgggtca ctcccgccac cgtcagggcg gcgatgcaga agtggctgac ccggccggtc
     1741 tacgcgctcc gggtggagcc cggcgagcgc aaggcctatg aagaggcgaa aggctcgcgc
     1801 ggcggcgccg ccagccagcg gccccgttac tatcgccagc cgcggccggg cgagaagccg
     1861 atggcgcctc tgcccttcgt cgcgccggac gagaacgccc ccgagccgct ttcctaccag
     1921 aagatcccga tgccggaggt cggcgagatt tcgaacctcg acttccccga cgtcgagcgc
     1981 gcgcggctgt cgaacggcat tcccgtcgtc tacgcccgcc gctcggcggt gccggtggtt
     2041 cgggtggcgg tcgagttcga cgccggggtc gccgccgatc cgggcgatgc gctcggcacc
     2101 cagtcgctga tgctgagcct gctcgaagag ggcacgacca gccgcaactc gatccagatc
     2161 gccgaggagc aggagcggct cggcgccgcg atcgggcccg aaacctcgct cgaccgcacg
     2221 gccgtgagcc tggccgcgct gactcccaat ctcgggccgt cgctcgacct tctcgccgat
     2281 atcgtccgca atccggcctt cgatcccggc gaggtcgagc ggctgcgcgc ccagcagctt
     2341 tccgccatcg atcaggagct gaccgagccg agcgggctcg gcgcgcgagt cctgccgccg
     2401 ctcctgttcg gagcgaacca tccctacgga cggccgtcca gcggcctcgg cgacaagccg
     2461 gtcatcgccc ggctcacccg cgacgacctg atccgcttcc accagagctg gatccgcccc
     2521 gacaacgcca ccatcttcgc ggtcggcgac ctgccgctcg ccgaattggt gccgcagctc
     2581 gaggcgcggt tcggcaactg gtcgcctccg gcggcgccga aggggaccaa gaatttcgcc
     2641 gccaccccgc cggcgccgaa gccgcggatc gtcctcgtca accggccgca atc
//
LOCUS       DASWNU010000340         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_64120, whole genome shotgun sequence.
ACCESSION   DASWNU010000340 DASWNU010000000
VERSION     DASWNU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64120"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGB57_06805"
     CDS             <1..499
                     /locus_tag="VGB57_06805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0131097.1"
                     /translation="IGTAEWDAMWVFYQDTGSRKWGHPYLTRDFFGRIAQTMGDRLLL
                     FLARRDGLPVAGALNLIGPGTLYGRYWGAIGEVPFLHFELCYYQAIEWAIAKGLSSVQ
                     AGAQGEHKLARGYEPVISRSAHFIADSGFRSAVAVFLEEERRAIAAEAQWERSALPYR
                     SSASE"
     gene            complement(475..1425)
                     /locus_tag="VGB57_06810"
     CDS             complement(475..1425)
                     /locus_tag="VGB57_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY0131098.1"
                     /translation="MTTAAPAHPPTEWKLVIHGGAGVIQRSSTTPAKDREIRAALDHA
                     LATGSRLLEAGGKALDAVEATVRELEDNSNFNAGRGAVFTFDGTHELDASIMDGSNRA
                     AGAVAGVTKTKNPISLARAVMDKSQHVFLSGAGADLFSREQKLEQVPNDYFSTPLRRQ
                     QLDELKAKGANFDSTMKYGTVGAVALDRHGHVAAATSTGGLTGKRWNRIGDSPVIGAG
                     TYADDRACAVSATGAGEYYIRVGAAHEICARIRLGREAPQAAADAVMKEIKDLGGRGG
                     VIVLTPKGERSWSFTTPGMYRGMADENGRQVALYSEAEER"
BASE COUNT          220 a          506 c          500 g          283 t
ORIGIN      
        1 aatcggcacc gccgagtggg acgcgatgtg ggtcttctac caggacaccg gtagccgcaa
       61 atggggccat ccctatctga cccgcgactt ctttggccgc atcgcgcaga cgatgggcga
      121 ccggcttttg ctgttcctcg cgcggcgcga cggcctgccg gtcgccgggg cgctcaatct
      181 gatcggtccc ggcacgcttt acggccgtta ttggggggcg attggcgagg tgccgttcct
      241 ccatttcgag ctttgctatt accaagcgat cgaatgggcg atcgccaagg gcctgtcgtc
      301 cgtgcaagcc ggcgctcaag gcgagcacaa attggcccgc ggctatgagc cggtgatttc
      361 ccgctcggcg catttcatcg cggactccgg ctttcgcagc gccgtcgcgg tcttcctcga
      421 agaggagcgc cgcgccattg ccgccgaagc ccaatgggag cgctcggcgc ttccctaccg
      481 ctcctcggct tcggaataga gcgcgacctg gcggccgttt tcgtcggcca tgccgcggta
      541 catgccgggg gtggtgaacg accacgaccg ctcgcccttg ggggtcagga cgatcacgcc
      601 tcccctcccg ccgagatcct tgatttcctt cataaccgca tcggccgcgg cttgcggtgc
      661 ctctcggccg agccggatcc gggcgcagat ctcgtgggcc gccccgacgc ggatgtaata
      721 ttctcccgcc ccggtggccg agaccgcgca ggcgcggtcg tcggcatagg tgccggcgcc
      781 gatcaccggg ctgtcgccga tccggttcca tcgcttgccg gtcagcccgc cggtcgaagt
      841 cgcggcggcg acatggccat gccggtcgag cgccacggcg ccgaccgtcc cgtatttcat
      901 cgttgagtcg aaattggcgc ctttcgcctt gagctcgtcg agctgctggc gccgtagggg
      961 tgtggagaaa taatcgttcg gtacctgctc cagcttctgc tcgcgcgaga aaaggtcggc
     1021 gccggcgccg ctcaggaaca cgtgctggct cttgtccatt accgcccggg ccaggctgat
     1081 cggattcttg gtcttggtga cgccggcgac ggccccggcc gcgcggttgc tgccgtccat
     1141 gatcgacgcg tcaagctcgt gcgtgccgtc gaaagtgaag accgcccctc ggccggcatt
     1201 gaaattggaa ttatcttcga gttcgcggac cgtggcctcg accgcgtcga gagccttgcc
     1261 gccggcctcg agcagccgcg acccagtcgc cagggcatgg tcgagagcgg cgcggatttc
     1321 acggtccttg gccggagtcg tcgaagagcg ttggatgacc cccgcaccgc cgtggatcac
     1381 cagcttccac tcggttggcg gatgtgcggg cgcggcggtc gtcatcagca acactccgag
     1441 gatcaggggc aaacgcatgg gaatctccct tgtcgcagga gccagagccg ggcgccccgg
     1501 cggattcca
//
LOCUS       DASWNU010000341         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_322714, whole genome shotgun sequence.
ACCESSION   DASWNU010000341 DASWNU010000000
VERSION     DASWNU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322714"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="VGB57_06815"
     CDS             complement(<1..1699)
                     /locus_tag="VGB57_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0131099.1"
                     /translation="MAIYNGDGSPNLFAGGVEADTANGDGGNDILSGGGGSDTLDGGD
                     DDDELSSSEKSPLFNVPWRDVPYTAPLLDTGIEVDHLQGGSGDDKLFAGYGDDVDGGA
                     NGSRGDNLYISFLGASAGVVVDFHLATQVVGGGTIQNIENAAWVQGTNYADDIDAGFD
                     VINNSEFGAVFGMGGNDRLRAGWSTILLDGGTGDDILDGRTGNYLLQALRGGDGDDTL
                     YADLGSAKVFGGEGNDTIYANGLISGGGGNDVIVLPAWNPGWLVAGDAGDDDITGSPD
                     WDNYISGGEGADVVRGGGSADRLASDVFSESSNYVDLGLDHDVLTAGAGDDFVTIGFG
                     DDADGGTGTDTLWLSLAGSASGVTLNTQLLASGQPVAIGGGTIQNFENIEYLVGSAYG
                     DTFNIATQAMWMLVSTGDGDDVVISNGSRLNLYMGEGNDLFYSGPVHDLFNGGPGTDT
                     VDYGGAAAGLVVILGFGGEWHPDSGGDTLVYVENVVGTGFADTLTGNELANILAGSGG
                     DDILTGNAGNDILDGGVGIDTMRGGANDDVYHVDNAGDLVEENAGAGTDEVRTSLASA
                     "
BASE COUNT          372 a          794 c          736 g          395 t
ORIGIN      
        1 tggcgctcgc gagagaggtg cggacctcgt ccgtgcccgc gcctgcattc tcctcgacga
       61 gatcgccggc attgtcgacg tgatagacgt cgtcattggc gccgccccgc atcgtgtcga
      121 tgccgacgcc gccgtccagg atatcgttgc cggcatttcc cgtcaggatg tcgtcgccgc
      181 cgctcccggc gaggatattg gccagctcgt tgccggtcag ggtgtcggcg aagccggtgc
      241 cgacgacgtt ctcgacatag accagggtgt caccgccgga gtcggggtgc cattccccgc
      301 cgaagcccaa aatcaccacc aggcccgcgg ccgcgccgcc gtaatcgacc gtgtccgtcc
      361 cggggccgcc gttgaacaga tcgtgcacgg gcccgctgta gaaaagatcg ttgccctcgc
      421 ccatgtacag attgagccgg ctgccgttgg agataacgac gtcgtcgccg tcccccgtcg
      481 acaccagcat ccacatcgcc tgggtcgcga tgttgaaggt gtcgccgtaa gcgcttccca
      541 ccagatattc gatattctcg aaattctgga tcgtcccgcc gccgatcgcc accggctggc
      601 ctgaggcgag gagctgggtg ttcaaggtca cgcccgaggc cgagccggcc agcgacagcc
      661 acaaagtatc ggtgcccgtc ccgccgtcgg catcgtcgcc aaagccgatc gtgacgaagt
      721 cgtcgcccgc tccggccgtc agaacgtcat gatcgagccc caggtcgaca taattgctgg
      781 attcggaaaa aacgtccgag gccagcctgt cggcggaacc gccgccgcgc acgacgtcgg
      841 ctccctcgcc gccggagata taattgtccc aatccggcga gccggttatg tcgtcgtcgc
      901 cggcatcgcc ggccaccagc cagccgggat tccaggccgg aagaacgatg acgtcgttgc
      961 cgccgccgcc gctgatgagg ccgttggcgt agatcgtgtc gttgccctcg ccgccgaaga
     1021 ccttggcgct gccaagatcg gcgtagaggg tgtcgtcgcc gtcgccgccc cggagggcct
     1081 gaagcaggta gttgccggtg cggccgtcga ggatgtcatc gccggtgccg ccgtcgagca
     1141 ggatggtcga ccatccggcc cgcaggcggt cgttgccgcc catcccgaac accgccccga
     1201 attccgaatt gttgatgacg tcgaatccgg catcgatgtc gtcggcatag ttggtgccct
     1261 gtacccaggc ggcattctcg atattctgga tcgtgccgcc gcccaccacc tgggtggcga
     1321 ggtgaaaatc gacgacgacg cccgcggacg cgccgaggaa gctgatgtac agattgtcgc
     1381 cgcgggaccc gttcgccccg ccgtcgacat cgtcgccata gcccgcgaac agcttgtcgt
     1441 cgccgctgcc cccctgcagg tggtccacct cgatacccgt atcgagcagc ggcgccgtgt
     1501 aggggacatc gcgccagggc acgttgaaca acggcgattt ttcggagctg gagagctcgt
     1561 cgtcgtcgtc gcccccgtcg agcgtgtccg aaccgccgcc gccggagaga atgtcgttgc
     1621 cgccatcgcc gttggcggtg tcggcctcga ctccgcccgc gaaaaggttc ggcgaaccgt
     1681 cgccgttgta gatagccatg attcgaaccc ccacgtcccg ctcggaaccc ggtgtccggc
     1741 cgctcaaggc taagtgggtc cgggaaccgg cgcaagcctc gggtccgaat gcatcgcgat
     1801 attggaaggt ggtagcggag gcccgctacc ggcgacactg acacggcggc gttcatttaa
     1861 ggcttgaagc ccccgcggcc gtcggcgcct cgatgcgcga actggccgcg gggacccccg
     1921 tgcgtggacg cacgcccagg ttgggccgcg ctcgccagca ccttgccttg agaaccagac
     1981 gctaggcggc cgcccaccct ctcccactgc gctttaacgc aggttcgtct gcgagccgcg
     2041 cggctctgga cccgtccgga gccggcggtt tcgtgcgtcc atcgacttcc gcacgaggag
     2101 gccgaaatgg tattgctcgc ccgaacggca agccgccccg tttcttaccc gattttcctt
     2161 tccacccagc acctggagcg tcgatcttga gcgcccaggg gggagccgct gacgcggcag
     2221 cgatctatgc ccggcccgac agcgtcccgg tcggggtcgt cgagcaccgg atctggaagc
     2281 cattcggcgc gacgaag
//
LOCUS       DASWNU010000342         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_141995, whole genome shotgun sequence.
ACCESSION   DASWNU010000342 DASWNU010000000
VERSION     DASWNU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141995"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..>1556)
                     /locus_tag="VGB57_06820"
     CDS             complement(118..>1556)
                     /locus_tag="VGB57_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0131100.1"
                     /translation="SRFEALGLKPAGRDGWFQQVPFLETRLSEEPGSLAIGGRRFVHG
                     QEVVLTPTPDQAPVSVEAPAVFVGYGLEAPEIGIDDYKGLDVRGKVVVVLGGFPNGMK
                     SDVGAHLNSDKRRVASSHGAVGLVSIRTLSEAKRRPWARLQQTADEPSLGWIGEDGRS
                     YSAAPGLRFRATLDTAAADALFAGAKTPLSRVLAEADRKGGRPKGFALKPRIAVSQQS
                     ASRRFSSPNVLAVLPGSDPKLAGEYVLLMGHLDHEGMKASGAEETGGDRIYNGAMDNA
                     VGIATMLEVARAMANSADRPKRSILFAAVTAEEKGLLGAQYLARNPLPGINVVGVVNL
                     DMPVLLYDFTDVIAFGAEHSTMGAAVARAGKGMGVALSEDPLPQEGLFTRSDHYRFVQ
                     EGVPSVFLMTGFANGGREKFTDFLSTHYHKVSDQLSLPIDWRAGAKFARINYEIAREI
                     ADAPQAPRWYADSFFGKALAPGREKAQR"
BASE COUNT          252 a          522 c          521 g          261 t
ORIGIN      
        1 ctcggattgt tcagcgaagt tggacatgag ttcgcgcccc tccgaccagg tcagggaacc
       61 gcaatgcctc agaatgagga gggccgcaaa ctctccggtc tgccgcccct tcgacgatca
      121 acgctgggcc ttctcccgtc ccggcgccag cgcctttccg aagaagctgt ccgcatacca
      181 gcgcggggcc tgcggcgcgt cggcgatctc gcgggcgatc tcgtagttga tccgggcgaa
      241 tttggctccg gcccgccagt cgatcggaag gctgagctgg tcgctgacct tgtgatagtg
      301 ggtcgagagg aagtcggtga acttctcgcg gcctccattg gcgaagccgg tcatcaggaa
      361 gacggaagga acgccctcct ggacgaatcg gtaatggtcg gatcgggtga agaggccctc
      421 ctgcggaagc ggatcctccg acaacgccac tcccatgccc ttgccggcgc gggccacggc
      481 cgcgcccatg gtcgaatgct ccgctccgaa ggcgatgacg tcggtgaaat cgtagagcag
      541 gaccggcatg tcgaggttga cgacccctac gacgttgatt ccgggaagcg gatttcgggc
      601 gagatactga gctccgagca ggcccttctc ctcggcggtc accgcggcga acaggatcga
      661 gcgcttgggg cggtcggccg aattcgccat ggcgcgggcc acctccagca tggtggcgat
      721 cccgacggca ttatccatcg cgccgttgta gatccggtcg cccccggtct cctcggcgcc
      781 cgaggctttc atcccttcat gatcgaggtg ccccatgagg agcacatatt cgccggccag
      841 cttcgggtcc gatcctggca gcacggcgag cacgttcggg cttgagaagc ggcgcgaggc
      901 gctctgctga ctgacggcga tccgcggctt cagggcgaag cctttgggac gcccgccttt
      961 gcggtccgct tctgcgagca ctcggctgag cggggtcttc gctccggcga agagggcgtc
     1021 ggcggcggcc gtgtccagag tggcccggaa gcgcaggccc ggcgccgcgc tgtaggaacg
     1081 gccgtcctcg ccgatccagc cgaggctcgg ctcgtcggca gtttgctgca agcgggccca
     1141 gggccggcgc ttcgcttccg acagagtgcg gatggagacc aacccgaccg ctccatgact
     1201 ggaagccacc cgccttttgt cggaattgag atgggcgccg acgtcgctct tcatcccgtt
     1261 cgggaacccg ccgagaacga cgacgacctt accgcgcacg tcgagcccct tgtaatcgtc
     1321 gattccgatc tccggcgcct cgaggccgta gccgacgaag acggccggcg cctcgaccga
     1381 gacgggcgct tggtccgggg tcggcgtcag gacgacctcc tgcccatgaa cgaaccggcg
     1441 tccgccgatc gcgagcgatc cgggctcctc ggaaagccgg gtttcgagga aggggacctg
     1501 ctggaaccag ccatcccgcc ccgccggctt cagccccagc gcctcgaagc ggctcg
//
LOCUS       DASWNU010000343        12061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_64267, whole genome shotgun sequence.
ACCESSION   DASWNU010000343 DASWNU010000000
VERSION     DASWNU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64267"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGB57_06825"
     CDS             complement(<1..221)
                     /locus_tag="VGB57_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0131101.1"
                     /translation="MFTDSHCHLNYKGLVEDQQAVLERARSAGVGTMLNISTRQSEWD
                     EVIGLAERESDVWASVGIHPHEADAHPDV"
     gene            475..1452
                     /locus_tag="VGB57_06830"
     CDS             475..1452
                     /locus_tag="VGB57_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0131102.1"
                     /translation="MARPRLAVVIPSFRAAGTIGAVLRAIGPEVGQIYVVDDGCPDST
                     GERALREISDPRIVLLRNKRNLGVGGAMKRGYARALADGAEIIVKLDADGQMDPRHIR
                     RLIAPIVHGVADYAKGNRFAPPRLMPSGSSPVALDSMPPARRAGNMALSVLHKAATGY
                     WRIGDPANGYTAVHARALERIGLEALADCFFFETDMLFRLNLVDAVVADVALPACYPG
                     SGSSLSLRRVAPRFAWMTASRFVRRLRAKYFSGAWNLGSVKLLAAAAMIAAAAGFAGW
                     RWIEPLSGSGSARAAATCFLLGLALLAAAGLYDARKAAREPLSRRGLHC"
     gene            1483..2640
                     /locus_tag="VGB57_06835"
     CDS             1483..2640
                     /locus_tag="VGB57_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131103.1"
                     /translation="MAAVAPPRPWVAALLIAAGHIGHVLARGATMAPDSRAYAYWSAR
                     LIESGFDYPALLAEASQAFPPILYALFASLLALLRLTFGGDWANGLVALNFAAHVALG
                     VLVVRLANRVTGSGAAGWGALLLFLGCFDLLMWVPLVLSDATFVFLAFLIFTLAAGRI
                     LDSSKSWLAVLAPAAAGIFYRPTGMVLIPDLIWAIYLSKTGTGPVRRGPVLAVLAAAM
                     LAGTLIFAWFMQDPGRWPLDFLSPAFRAIAEGYSGGEVVNARFETYHMPPRTILDYLL
                     ISADRFLHFFAIGASTYSLGHWLVELVFFLPCYALAGWLAVALWRGRTGFGAGERKVF
                     LAAFGAILSYAVFHALVQVDFDWRYRTPILPHFILLASGGLADLMRKRSAE"
     gene            2637..3488
                     /locus_tag="VGB57_06840"
     CDS             2637..3488
                     /locus_tag="VGB57_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0131104.1"
                     /translation="MSGETPFLFSVDVEDPRLDLPGGERLPARVPALIDTYLDFLRRH
                     GAKGTFFVVGEVARRHPEAIARIAAEGHELGCHSDAHVPLDRLGQAGFRDDLRRNLDA
                     LRAAGADRIEGYRAPCFSLTAETGWAYEKLAELGFSYSSSVLPARNPLYGNPAFGTAP
                     RLIEGIVELPVTLLPFRMLPVPVGGLYFRVMPRPILRRALAGRLRRGEAVASYHHPYD
                     IDTEQSFTHAGFRRWGAFDLLMRANRGAVLPRLEMVGALGFAFAPYGPHARHLGEALR
                     SHRGAGG"
     gene            3481..4329
                     /locus_tag="VGB57_06845"
     CDS             3481..4329
                     /locus_tag="VGB57_06845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0131105.1"
                     /translation="MADDYRFEPLEHSARGYEHISRLMSEVFPKARHLTPRYFEWHYG
                     RNPNGQAIGCNAFSGDELVGHMAAHVFPCRLEGEERRGMYTVNGSVHPLHRGRRLQSR
                     ISAAMFEEAARLGYAFCLATGNKWSTGPLLTRFKMIRPLDARLGFGLPKRRENGPQAS
                     FERIWSDEALRWRLANPEGRYSAVRRGDRSAILASTGLPGLGAILHQGPARDTLAGSG
                     PAPGPTRLWLGLDPGVDWSGSAFLPIPMRLRPSPLNLVYKDLTGGDFVPDPDRVVFRG
                     LDFDAF"
     gene            4442..4807
                     /locus_tag="VGB57_06850"
     CDS             4442..4807
                     /locus_tag="VGB57_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131106.1"
                     /translation="MTDERFDPTDPERRGAMLGEAIDGAVRKITTGIVIAGGLIALGI
                     YAHPEPRFDAFALGDQIIRVDGKTGTIIACEGRQTCQLVLRKGQSLERIKRTNSSSRP
                     ALPRPAQATPPQAAPAAGK"
     gene            complement(4770..6332)
                     /gene="metG"
                     /locus_tag="VGB57_06855"
     CDS             complement(4770..6332)
                     /gene="metG"
                     /locus_tag="VGB57_06855"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0131107.1"
                     /translation="MAEPFYITTAINYPNGPPHIGHAYEAVATDVIARFQRAMGREVF
                     FQTGTDEHGLKMAQAARERGIEPAELAEEMSSIFKVMDERLQISFDRFIRTTEPAHHR
                     ASQAIWEAMAARGDLYLGRYEGWYSVRDEAYYDEGELLAGGNGEKLSPQGTPVEWTVE
                     ESWFFRLSAYQDRLLAHYSEHPGFIRPESRRNEVLRFVEGGLSDLSVSRTSFDWGVKV
                     PGDERHVMYVWLDALTNYITGVGYPDTESEAYRRYWPADIHIIGKDVVRFHAVYWPAF
                     LMSAGLELPKQVFGHGHVLLRGEKMSKSAGNVVAPAELIDRFGADAVRYFLMRDVTFG
                     QDGSYSPEAIVARVNADLANSFGNLAQRTLSFIAKNCGGVLPTGGRGEGADSELLDLV
                     RRTTARDVPALFADLSLSQGIEAWIRAVFACNQYIDVQAPWTLRKTDPERMTAVLACL
                     CEAITDLAIAIAPVVPASATKLLDQMGVPPGERDFTVLADTGRYARLAASGAVLEAPT
                     PIFPRLELPAEA"
     gene            complement(6342..7319)
                     /locus_tag="VGB57_06860"
     CDS             complement(6342..7319)
                     /locus_tag="VGB57_06860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY0131108.1"
                     /translation="MTPLFGHHSAVAAFRDALDSGRLHHAWLISGSAGVGKALFAHKA
                     ALRVLAQGQGPVEAPGLEVPASHPAAHLVAAGSHPDLMRLERLPRENSSTGELARNIN
                     VEQVRSLQRLFNTTASLSAWRAVIVDSIDDLERNAANALLKNLEEPPPSSVFFLVSHA
                     PERLLPTIRSRCRLLRLSRLDADAMTSALRSALPDADDKEINDLAVAGNGSPGRALAW
                     RGLDVAGLDRAMAELIREGDPANARRSALAQSLALKSAQPRYEAFLARAPSVIAAEAK
                     DRRGPALAQALVAWERAQGLASSARGLSLEPESVVFELAGMLASLAPSR"
     gene            complement(7316..7939)
                     /gene="tmk"
                     /locus_tag="VGB57_06865"
     CDS             complement(7316..7939)
                     /gene="tmk"
                     /locus_tag="VGB57_06865"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0131109.1"
                     /translation="MRGRFISLEGGEGAGKSTQLRALAEALRGRGLEVVETREPGGSP
                     GAEAIRKLLLEGGDDRWTPEAEALLFAAARADHVARTIRPAVERGAWVVSDRFLDSSL
                     AYQGGAAGVGVETVRALHRIGSHGFLPDRTLLLELPTEAVAARLSIRDADGSDRIGGR
                     GGEFHRKVAQTFSKLAEQAPERFRRVDASGAAEEVTARLLAAVGDLL"
     gene            complement(7946..9112)
                     /locus_tag="VGB57_06870"
     CDS             complement(7946..9112)
                     /locus_tag="VGB57_06870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0131110.1"
                     /translation="MRKSLFPIAFTAAAALPATAAAPPFETAAPVAYMVDLSSGAVLY
                     SKDPDRRIPPASMAKMMTTHLAFHMIKRGEAKLDKMCQVRPETWRQWHGPQAGSTMFL
                     SPGEQVSVENLLHGIVTLSGNDATVVLAECLAGTEPAYAALMNAEAKRLGMANSNFGN
                     TNGWPDEGVTFTTARDLAKLAAATIQETPDLYKAFYQKKEFTWGKTLGAGAPITQGNR
                     NPLLGRVQGADGLKTGHTAEAGYGFTGSAEQGGRRIIMVVAGLGSFNQRIEESVKFMD
                     WGFRAWKSQPLFNQNEKVAEAQVQGGGDDIVPLVAPRSIAVTLPAGAAGSIRAKVVYN
                     GPIKAPIAKGQHVADLVVTTADTPPQTMPLVAAQAVGEAGFFGRMWNGLTSLFG"
     gene            complement(9203..10246)
                     /locus_tag="VGB57_06875"
     CDS             complement(9203..10246)
                     /locus_tag="VGB57_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY0131111.1"
                     /translation="MRLLRGIAALALTAAILPLSSSAPAQSGLSESGFRSYLPQLEAQ
                     ARRAGVSPGTIASVFPNLVFSARTVELDRAQPGGRPGSNANPPFAPYRARHVTPALVA
                     GGQRRYAANLSRLQAIGRRYGVTPSVLVAIWGHETSYGAVTGNFDLLNSLASLAYDGR
                     RRQLFSDEFVAALKMIDRGVPRYRLKGSWAGATGYPQFLPSVWLRLGSDGDGDGRSDI
                     WRSEPDALASIANYLNNAGWRAGVPWGVAVRVPAGFDRSRVRNTLRAPRCPRVYERHS
                     RWLTFGEWRRLGIVAQNGIWPRDSEMASLIEPDGPNATAYLLTTNYRTILDYNCSNFY
                     ALSVGLLADAIAR"
     gene            10453..10539
                     /locus_tag="VGB57_06880"
     tRNA            10453..10539
                     /locus_tag="VGB57_06880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10487..10489,aa:Ser,seq:tga)
     gene            complement(10568..11854)
                     /locus_tag="VGB57_06885"
     CDS             complement(10568..11854)
                     /locus_tag="VGB57_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY0131112.1"
                     /translation="MKFVWLWAALLALPAIGAAPRSALAQAPGPLPVQSASSRQVLAA
                     IAAPAEGQAAYLTEPGREGWFRFERRPSEAEIAADPAQALFVRGAGGTWVRIYSGKVD
                     ARWFGARGDGVSDDTAALQRAIDHVAKGSGGGIVIPPGVYRVTNLTVPAYPVAEGNSL
                     KVVEITGTSMPVTQFGSIGAFPVSNEGTIIKSTATAGGAVLKVAGSPGFGGFSWYHVV
                     LEDLNFRTYDDPQIGAVDMGNAAQLTAVNLQIDSGIYPVQASRPTHKTAGLVTPRNNN
                     AALTTLRNINIMGYYNLLEVNEHTDADQIILGAGWHGLLFNEGHHASRLGRVMAYRVN
                     TPVAFAGVHSFSIEQLDIEHTITAAEATDPADLSIGNGYQVTQEDFRDVSNQGLGYVN
                     WHVVQGNVGRVDKFTVNGAKNVQFRQLGKAVPAVSR"
BASE COUNT         1771 a         4051 c         4235 g         2004 t
ORIGIN      
        1 tggacgtcgg gatgcgcgtc ggcttcgtgg ggatggatac ccacggaggc ccagacgtcg
       61 ctctcgcgct cggcgaggcc gatcacctcg tcccattccg actggcgggt cgagatgttg
      121 agcatggtgc cgacgccggc ggagcgggcg cgctccagca cggcctgctg gtcttcgacc
      181 aggcccttat agttgaggtg gcaatggctg tcggtgaaca tggagattcc ttagccccat
      241 cccctcccct ccacgcaagc gccgtctgcc gttcggcttc cggcgcgccc gatccggcgg
      301 ttcgtctcat tccctgtcag cagcgccgat caagccactg gcgcgggtgc tcctttctgc
      361 taggagccgc gcacgactga tttgcgttaa gtgcttcgca cgtgggggcg tgccggcatg
      421 ggggtaagaa tgaagccgga ccatgcgttc cctcccgtaa ccggtgacac cggcgtggcg
      481 aggccgcggc ttgccgtggt gatcccttcc tttcgagccg ccgggacgat cggcgccgtt
      541 cttcgcgcca tcggccccga ggtcggccaa atctacgtgg tcgacgacgg ctgccccgat
      601 tccaccggcg agcgggcgct gcgggagatt tccgacccgc gaatcgtcct gctccgcaac
      661 aagcgcaacc tgggagtcgg cggggcgatg aagcgcggct atgcgcgcgc tctcgccgac
      721 ggcgcggaga tcatcgtcaa gctggacgcc gacggacaga tggacccgcg ccatatccgc
      781 cggctgatcg cgccgatcgt ccatggcgtc gccgattatg ccaagggcaa ccgcttcgcc
      841 ccgccaaggc tgatgccttc gggaagctcc ccggtggcgc tggattcgat gccgccggcg
      901 cggcgggccg gcaacatggc cctgtccgtg ctccacaagg ccgccaccgg ctattggcgg
      961 atcggcgatc cggccaacgg ttacaccgcg gtccatgccc gggcgctcga gcggatcggc
     1021 ctcgaggcgc tggcggattg cttcttcttc gagaccgaca tgctcttccg cctcaacctg
     1081 gtcgacgccg tggtcgccga cgtcgccctt cccgcctgtt atccggggtc ggggagcagc
     1141 ctcagcctgc gccgggtcgc cccccgcttc gcctggatga cggcgagccg cttcgttcgc
     1201 cgcctgcgcg ccaaatattt cagcggcgcg tggaatctcg gctcggtcaa gctgctggcg
     1261 gcggcggcga tgatcgcggc cgccgccggc ttcgcgggct ggcgctggat cgagcctttg
     1321 agcggctccg ggtcggcccg ggcggcggcg acctgctttc tgctcggcct cgccctgctc
     1381 gcggcggcgg ggctctacga cgcgcgcaag gccgcccgcg aaccgctctc gcgccggggc
     1441 ttgcactgct gaatgcctgc ggtggtgcca atgcgggatc ggttggccgc ggtcgctccg
     1501 ccgcgcccgt gggtggcggc gctgctgatc gcggcgggcc atatcggcca tgtgctggcg
     1561 cgcggagcga ccatggctcc ggacagccgg gcctacgcat attggtcggc gcggctgatc
     1621 gagagcggct tcgactatcc ggccctcctc gccgaggcga gccaggcctt cccgccgatc
     1681 ctctacgcgc tgttcgcgag cttgctggcg ctgcttcgac tgacgttcgg cggcgattgg
     1741 gcgaacggcc tggtggccct gaatttcgcc gcccatgtcg cgctcggcgt gctcgtcgtg
     1801 aggcttgcga accgggtgac cgggagcggc gcggccggct ggggggcgct gctgctcttt
     1861 ctcggctgct tcgacctgct catgtgggtt ccgctcgtgc tttcggacgc caccttcgtc
     1921 tttctcgcct ttctgatctt cactctcgcc gccgggagga tcctcgactc gtcgaaatcc
     1981 tggctggcgg tcctggcacc ggccgccgcc gggatcttct accggccgac cgggatggtc
     2041 ctgatccccg acctcatctg ggcgatctac ctctccaaaa ccgggaccgg gccggtccgg
     2101 cgagggccgg tgctggcggt cctcgccgcc gccatgctgg ccgggacctt gattttcgcc
     2161 tggttcatgc aggatcccgg ccgctggccg ctggacttct tgtctcccgc cttccgcgcc
     2221 atcgcggaag gatattcagg cggcgaggtg gtgaacgccc ggttcgaaac ctatcacatg
     2281 ccgccgcgga caattctcga ctacctgctc atctccgccg accgcttcct ccacttcttc
     2341 gcgatcggag cgtcgaccta cagcctcggc cactggctgg tggagctggt cttcttcctg
     2401 ccctgctacg ccctggcagg ctggctcgcg gttgcgctgt ggcgcggccg gaccggcttc
     2461 ggagccgggg agcgcaaggt cttcctggcc gccttcggcg caattctttc ctacgccgtc
     2521 ttccacgccc tcgttcaggt ggatttcgac tggcgctacc gaaccccgat cctgccccac
     2581 ttcatcctgc tcgcctccgg cggcctcgcc gacctcatga ggaagaggag cgccgaatga
     2641 gcggcgagac gcccttcctg ttcagcgtcg acgtcgagga tccgcgcctc gatctgccgg
     2701 gcggcgagcg gctgccggcg agggtgccgg cgctgatcga cacctatctc gatttccttc
     2761 gccggcacgg cgcgaagggc actttcttcg tggtcggcga ggtcgctcgc cggcaccccg
     2821 aggcgatcgc ccggatcgcc gccgagggcc atgagctcgg ctgccattcc gatgcccacg
     2881 tgccgctcga ccgcctcggc caggcgggct tccgcgacga tctgcggcgc aacctcgacg
     2941 ccttgcgggc ggccggcgcc gaccggatcg agggttatcg cgcaccctgc ttctcgctca
     3001 ccgccgagac cggctgggct tatgaaaagc tggccgagct cggcttttcc tattcgagct
     3061 ccgtgcttcc ggcgcgaaac ccgctctacg gcaacccggc cttcggcacc gcaccgcgcc
     3121 tgatcgaggg cattgtcgag ctgccggtga ccctgcttcc gttccggatg ctgccggtgc
     3181 cggtcggcgg gctttatttc cgggtcatgc cgcggccgat ccttcgccgg gcgctggccg
     3241 ggcgccttcg ccgcggcgag gccgtcgcca gctaccacca tccctacgac atcgacaccg
     3301 agcagagctt cacccatgcc ggcttccgcc gctggggcgc gttcgacctg ctgatgcggg
     3361 ccaatcgcgg cgcggtcctg ccgaggctgg agatggtcgg ggcgctgggc ttcgccttcg
     3421 ccccctacgg gcctcatgcc cggcacctcg gcgaagctct ccgttcgcat cgaggcgccg
     3481 gtggctgacg attatcgatt cgagccgctc gagcacagcg cccgcggcta cgagcacatc
     3541 tccaggctta tgagcgaggt gttccccaag gcccgccacc tgacgccgcg ctatttcgaa
     3601 tggcattacg gccggaatcc caacggacag gcgatcggct gcaacgcttt ttcgggcgac
     3661 gagctggtcg gccacatggc ggcccacgtc tttccctgcc gcctcgaagg tgaggagagg
     3721 cgcgggatgt acacggtcaa cggctcggtc catccgttgc accgagggcg aaggctgcag
     3781 agccggatca gcgccgccat gttcgaggaa gcggcgcggc tgggctatgc cttctgcctg
     3841 gccaccggca acaagtggag cacgggcccg ctcctcaccc gcttcaagat gattcggccg
     3901 ctcgacgccc ggctgggctt cggcctgccg aagcgccgcg aaaacggccc ccaggccagc
     3961 ttcgagcgga tctggagcga cgaggccctg cgctggcgac tggccaatcc cgaagggcgt
     4021 tattcggcgg tccgacgcgg cgaccgatcg gccatcctcg cctccaccgg cctgccgggt
     4081 ctcggcgcca tcctccatca gggccccgcc cgcgacactc tcgccggttc cggccccgcg
     4141 ccggggccga cccggctctg gctggggctc gatcccgggg tggattggag cggctccgcc
     4201 ttcctgccca tcccgatgcg gctgcgcccc tccccgctca acctcgtcta caaggacctg
     4261 accggcggcg acttcgtgcc cgatccggac cgggtcgtct tccggggcct cgatttcgac
     4321 gccttctaga gggacagccg gatgactgtc actcgactgt ccccaaatgt cgcgtcgcgg
     4381 acggttacgc acccgtagcg ataggctctg cagggaaggg caaaagcttg tggagggcgc
     4441 catgaccgac gagcgtttcg atccaaccga tcccgagagg cgcggtgcga tgctggggga
     4501 ggcaatcgac ggagcggtgc gcaagatcac caccggcatc gtcatcgccg gcgggttgat
     4561 cgccctcggc atctacgccc accccgagcc gcgcttcgac gctttcgccc tgggcgacca
     4621 gatcatccgg gtcgacggga agacagggac gatcatcgcc tgcgaggggc ggcagacctg
     4681 ccagctggtg ctccggaaag gccagagcct ggagcgcatc aagcggacca actcttcgtc
     4741 ccggcccgcc ttgcccaggc ccgcccaggc tacgcctccg caggcagctc cagccgcggg
     4801 aaaataggcg tcggcgcctc gaggacggcc ccggacgcgg ccaggcgggc gtagcggccg
     4861 gtatcggcga ggaccgtgaa gtcgcgctcg cccgggggaa cgcccatctg gtcgagcagc
     4921 ttcgtggcgg acgccgggac gacgggggcg atggcgatgg cgaggtcggt gatcgcctcg
     4981 cacaggcaag cgaggacggc cgtcatccgt tccggatcgg tcttgcgaag ggtccagggc
     5041 gcctggacgt cgatatattg gttgcaggcg aagacggccc ggatccaggc ttcgatcccc
     5101 tggcttaggg agaggtcggc gaacagggcc ggcacatcgc gagcggtggt tcggcggacc
     5161 aggtcgagaa gctcggaatc ggccccctcc cctcgtccgc ccgtcggcag gactccgccg
     5221 caattcttgg caatgaagga caaggtgcgc tgggcgagat tgccgaagct gttggcgagg
     5281 tcggcattga cccgggcgac gatcgcctcg gggctgtagc tcccgtcctg gccgaaggtg
     5341 acgtcgcgca tcaggaaata gcgcaccgcg tcggctccga aacggtcgat cagctcggcc
     5401 ggagcgacga cgttgccggc cgacttggac atcttctcgc cgcgaaggag gacgtggccg
     5461 tggccgaaca cctgtttggg aagctcgagg cccgccgaca tcaggaaagc gggccagtag
     5521 acggcgtgga aacgcaccac atccttgccg atgatgtgga tgtcggctgg ccaatacctc
     5581 ctgtaagcct ctgattcagt atcgggatag ccgacgccgg tgatgtagtt ggtgagcgcg
     5641 tccagccaca cgtacatgac gtggcgctca tcgcccggga ccttcacccc ccagtcgaag
     5701 ctggtcctcg agaccgagag gtcggaaagg ccgccctcga cgaatcgaag cacctcgttg
     5761 cggcggcttt ccgggcggat gaagccgggg tgctccgaat aatgcgcgag cagccggtcc
     5821 tgataggcgg agagccggaa gaaccagctt tcctcgaccg tccactcgac cggggtgccc
     5881 tgcggcgaga gcttctcgcc attcccgccg gccagaagct cgccttcgtc gtaataggct
     5941 tcgtcccgta ccgagtacca gccctcgtag cggccgagat agaggtcgcc gcgcgccgcc
     6001 atcgcctccc agatcgcctg gctggcgcgg tggtgggcgg gctcggtggt ccggatgaag
     6061 cgatcgaacg aaatctgaag gcgttcgtcc ataactttga atatagaaga catctcctcg
     6121 gcaagctcgg ccggctcgat tcccctttca cgcgccgcct gggccatttt gaggccatgc
     6181 tcgtcggtcc cggtctgaaa gaagacctcc cggcccatgg cgcgctggaa gcgggcgatg
     6241 acgtcggtgg cgacggcttc gtaggcgtgg ccgatatggg gcggcccgtt gggatagttg
     6301 atcgcggtgg tgatgtagaa gggctcggcc atggcagggc cttagcggct cggagccagc
     6361 gatgcgagca tgccggcgag ctcgaacacg acgctctccg gttcgagcga caggccgcgg
     6421 gcgctggagg cgaggccctg ggcccgctcc caggcgacga gggcctgggc cagagcgggc
     6481 ccgcgcctgt ccttcgcctc ggcggcgatg acggagggtg cccgggcgag gaaggcctcg
     6541 tagcggggct gggccgattt gagggcgagg ctctgggcga gcgccgagcg gcgggcattg
     6601 gccgggtcgc cctcccggat cagctcggcc atggcccggt cgagccctgc gacgtcgagt
     6661 ccccgccacg ccagcgcacg gcccggagag ccgttgccgg cgacggccag atcgttgatt
     6721 tccttatcgt ccgcatcggg aagggcggag cgaagggctg acgtcatggc gtcggcgtcg
     6781 agtcgggaga gtcggagcag gcggcagcgc gagcggatgg tgggcagaag ccgctcgggc
     6841 gcgtggctga ccaggaagaa gacgctgctg ggcggcggct cctccagatt cttgagcaag
     6901 gcgttggccg cgttacgctc cagatcgtcg atcgaatcga cgatcaccgc ccgccacgcc
     6961 gagagcgagg cggtggtgtt gaacagccgc tgcagcgagc ggacctgctc gacattgatg
     7021 ttgcgggcga gctcgcccgt cgagctgttc tcgcggggca ggcgctcgag ccgcatcagg
     7081 tcgggatggc tgccggccgc gacgaggtga gcggccgggt ggctggcggg cacttccagg
     7141 ccgggcgcct ccaccgggcc ctgcccttgg gcgagcacgc gcaaagcggc cttgtgggcg
     7201 aacaaggcct tgccgacgcc cgcggagccc gagatcagcc aggcgtggtg gagccggccc
     7261 gaatcgagcg cgtcgcggaa agcggcgacg gcggagtggt gaccgaagag gggggtcaca
     7321 gcagatcgcc gacggcggcg agcagccttg ccgtcacttc ctcggccgct ccgctcgcgt
     7381 ccactcgccg gaaacgctcg ggcgcctgct cggccagctt cgagaaggtc tgggcgacct
     7441 tgcggtggaa ctcgccgccc ctgcccccga tccggtcgga tccatccgcg tcgcggatgc
     7501 tgagacgggc ggcgacggcc tcagtgggca attcgagcaa caaagtgcgg tcgggaagga
     7561 agccatggct gccgatccgg tgcagggcgc gaacggtctc gacgcccacc cccgccgcgc
     7621 cgccctgata ggcgagcgag ctgtcgagga agcggtcgct caccacccag gcgccgcgct
     7681 cgacggcggg acggatcgtc ctggcgacat ggtcggcgcg ggcggcggcg aacagcaagg
     7741 cttccgcttc cggggtccag cggtcgtcgc cgccttccag cagcagcttg cggatcgcct
     7801 cggcgccggg gctgccgccg ggctcgcggg tttccaccac ctcgaggccg cggccgcgca
     7861 gcgcctcggc cagcgctcga agctgggtcg acttgcccgc gccctcgccg ccttcgaggc
     7921 tgatgaagcg ccccctcacc ccgcctcagc cgaacagcga cgtcaggccg ttccacatgc
     7981 ggccgaagaa gccggcctcg ccgaccgctt gcgcggcgac caggggcatg gtctggggcg
     8041 gcgtgtcggc ggtcgtgacg acgaggtcgg cgacgtgctg gcccttggcg atcggcgcct
     8101 tgatcgggcc gttatagacg accttggcgc ggatgctgcc cgcagcgccg gccggcaggg
     8161 tcaccgcgat gctgcgcggc gcgaccagcg ggacgatatc gtcgccgccg ccctggacct
     8221 gagcttcggc gaccttctcg ttctggttga acagaggctg cgacttccag gctcgaaagc
     8281 cccagtccat gaacttgacc gattcctcga tccgctggtt gaagctgccg aggcccgcga
     8341 cgaccatgat gatccgccgc ccgccctgct cggccgagcc tgtgaagccg tagccggctt
     8401 ccgcggtatg gccggtcttg aggccgtcgg cgccctgcac ccggcccagc aaggggttgc
     8461 ggttgccctg cgtgatcggg gcgcccgcgc ccagcgtctt gccccaggtg aattccttct
     8521 tctggtagaa ggccttgtag aggtcgggcg tctcctggat ggtggccgcg gcgagcttgg
     8581 caaggtcgcg cgcggtggtg aaggtgacgc cctcatccgg ccagccgttg gtgttgccga
     8641 aattgctgtt ggccattccc agccttttcg cttcggcgtt catcagcgcg gcataggccg
     8701 gctcggttcc ggcgaggcat tcggcgagga cgacggtggc gtcgttgccg gagagagtga
     8761 cgatgccgtg gagcaggttc tcgacgctga cctgctcgcc tggcgagagg aacatggtcg
     8821 atcccgcctg cgggccgtgc cactggcgcc aggtctcggg gcggacctgg cacatcttgt
     8881 cgagcttcgc ctcgccgcgc ttgatcatgt ggaaggcgag atgggtggtc atcatcttcg
     8941 ccatcgaggc cggcgggatg cgccggtccg gatccttcga atagaggacg gcccccgagc
     9001 tcagatcgac catgtaggcg accggggcgg cggtctcgaa gggcggcgct gcggcggtgg
     9061 ccgggagcgc ggcggcggcg gtgaaagcta ttggaaacag gcttttgcgg atcatggttg
     9121 agactggtcc cctgaagatg cggtgatatg gccgaagcgt cgacttatgc ttaacccctc
     9181 ccctcgcgcc gcgccagacg gatcagcgcg cgatcgcgtc cgcgagcagc ccgaccgaga
     9241 gcgcgtagaa gttcgagcaa ttgtaatcga ggatggtgcg gtaattggtg gtgagcagat
     9301 aagccgtcgc attcggcccg tcgggctcga tcagcgaggc catttcggaa tcgcgcggcc
     9361 aaatcccgtt ttgagcgacg attccgaggc gccgccactc gccgaacgtc agccagcggc
     9421 tgtggcgctc atagacccgc gggcagcgcg gcgcgcgcag ggtgttgcgg acccggctgc
     9481 ggtcaaagcc cgcggggacc cggaccgcga ccccccaggg aacgcccgcc ctccagccgg
     9541 cattgttgag gtaattggcg atcgacgcga gggcgtcggg ctcgctccgc cagatgtcgg
     9601 agcggccgtc gccgtcgccg tcggagccga ggcgaagcca gacggagggg aggaattgcg
     9661 gatatccggt agcgccggcc cagctgccct tgaggcggta cctgggcacg ccccggtcga
     9721 tcatcttgag ggcggcgacg aactcgtcgg agaagagctg ccgacggcgc ccgtcataag
     9781 cgagcgaggc gagcgagttc agaaggtcga aattgccggt gacggcgccg tagctcgtct
     9841 catggcccca gatggcgacg aggaccgacg gggtgacccc gtagcggcgg ccgatcgcct
     9901 ggagccgcga caggttggcg gcatagcggc gctgccctcc ggcgacgagt gccggggtga
     9961 cgtggcgggc gcgatagggg gcgaagggcg gattggcgtt gcttccgggc ctgccgccgg
    10021 gctgggcgcg gtcgagctcc acggtgcggg cgctgaagac gaggttgggg aagacgctcg
    10081 cgatcgtccc tgggctgacc ccggcacggc gcgcctgggc ctcgagctgg ggcagatagg
    10141 atctgaagcc ggactcgcta aggccgctct gggccggcgc cgagctcgac aggggcagaa
    10201 tcgccgccgt cagggccagg gcggctatcc ccctcaacaa acgcatacat ttttcctccc
    10261 actccgcgcc actgatgcca caggggaacc gccggagata ctcggctcgc cgcgcgggct
    10321 gcacgaacgg ccccaccccg ctcgtcatcg cggcgcaagc cgggacctcg gcgctgaagg
    10381 acgccgcgcc tctttcgcga cgaggccccg gcttccgccg gggtgacggg taggctagag
    10441 gccctgccgt ccggacaggt ggccgagtgg tttaaggcag cggtcttgaa aaccgccgtg
    10501 ggtgcaagcc caccgtgggt tcgaatccca ccctgtccgc cgatttcgtg ccgccggccg
    10561 gccgcaccta tcgggaaact gccgggacgg ccttgccgag ctgtcgaaac tggacgttct
    10621 tcgcgccatt gacggtgaat ttgtcgaccc ggccgacatt gccctggacg acatgccagt
    10681 tgacgtagcc gaggccctgg ttcgagacgt cgcggaaatc ctcctgggtg acctgatagc
    10741 cgttgccgat gctgagatcg gcggggtcgg tggcctcggc ggcggtgatc gtatgctcga
    10801 tgtccagttg ctcgatcgaa aagctgtgga cgccggcgaa ggccacgggg gtgttgaccc
    10861 ggtaggccat cacccggccc agcctggagg catgatggcc ttcgttgaac agcaggccgt
    10921 gccagccggc gccgaggatg atctggtcgg cgtcggtatg ctcgttgacc tcgagaagat
    10981 tataatagcc catgatgttg atattgcgaa gcgtggtgag cgccgcattg ttgttgcgcg
    11041 gagtgacgag ccccgcggtc ttgtgcgtgg gccgggacgc ctggacggga tagattccgc
    11101 tgtcgatctg aaggttgacc gcggtgagct gagcggcgtt gcccatgtcg accgcaccga
    11161 tctgcggatc gtcataggtg cggaagttca ggtcctcgag cacgacgtgg taccaggaaa
    11221 aaccgccgaa gccgggggat ccggcgacct tgagcacggc cccaccggca gtggccgtgg
    11281 atttgatgat ggtcccttcg ttgctgaccg ggaacgcgcc gatcgagccg aattgggtga
    11341 ccggcatgga ggtgcccgta atctcgacga ccttgagcga attgccctcg gcgacgggat
    11401 aggccgggac ggtcaggtta gtgacccgat agacacccgg cgggatgacg atgccgcctc
    11461 ccgaaccttt ggcgacatgg tcgatcgcgc gctggagcgc cgccgtatcg tcgctgacgc
    11521 cgtcgcccct ggcgccgaac catcgggcgt cgaccttgcc cgaatagatg cgcacccagg
    11581 tgccgccggc gccgcggacg aacagggcct gggccggatc ggcggcgatt tcggcctcgc
    11641 tggggcgccg ttcgaagcgg aaccagcctt cccgccccgg ctcggtcaaa taggcggcct
    11701 ggccctcggc gggggcggcg atggcggcga ggacctgccg gctcgaggcg ctttgcacgg
    11761 gcaagggtcc gggcgcctgc gcgagtgcgc ttcgcggagc ggctccgatc gcgggcaacg
    11821 ccagcagggc cgcccatagc cagacgaatt tcatagggcc tcctttgcgg gtcgctatgc
    11881 cgcagcggcg cgggagtgga cagcccgccc gcgattggcg aaagggaccc catccaacgc
    11941 agccgaggat ttcaatggcc gacccccgcc ccctcccctt cgactgggcc gatcctttcg
    12001 cgctcgacgc gcagcttacg gacgaggagc ggctggtccg cgacaccgcc gaggcctatg
    12061 c
//
LOCUS       DASWNU010000344         4408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_91051, whole genome shotgun sequence.
ACCESSION   DASWNU010000344 DASWNU010000000
VERSION     DASWNU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91051"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1012
                     /locus_tag="VGB57_06890"
     CDS             155..1012
                     /locus_tag="VGB57_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0131113.1"
                     /translation="MRDSTPPFRRLGPEKPSSPVLVSVPHAGRAYSDGLLASARLSRA
                     RLESLEDRLVDRLVWRAVEGGAAALIADAPRAEIDLNRDERELDPAMVQPRPAAHLTV
                     ESPRTRGGLGLIPARIAGSGAIWRQRIAAAEVARRVEHIHRPYHAALEAELRSVKARF
                     GIAILLDCHSMPPRGGEGEAPVVLGDRHGGSIAEALVAAAEGAVRAAGFKVARNAPYA
                     GGHITERHGRPACGFHALQLELDRSLYLAPDLRTTGPGFDRVARLIAAIAEALAAAAL
                     DPPAAIAAE"
     gene            1014..1544
                     /locus_tag="VGB57_06895"
     CDS             1014..1544
                     /locus_tag="VGB57_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131114.1"
                     /translation="MRGRLLLLAPAVLLGAAPAAARPPVETPRLLSNGWHYGEAGNYC
                     MAIRQLAGGERLSMRLAKWDDHSDSLILWAAGLPPLDLDDEAAAERHYDLEVRIDGRR
                     IPRAMHSNMLEEFDGKPGPSYRLGIAQKPFIAALAKGRRLEIERAGKLVRAFPIAGSA
                     AMARLFADCVEKDPSF"
     gene            1612..1776
                     /locus_tag="VGB57_06900"
     CDS             1612..1776
                     /locus_tag="VGB57_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131115.1"
                     /translation="MISKRTYLAAAALIALAAFATGQAFPGMGVPFLVLASTLWVAWT
                     VRRGGRRGLR"
     gene            1914..2261
                     /locus_tag="VGB57_06905"
     CDS             1914..2261
                     /locus_tag="VGB57_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131116.1"
                     /translation="MPRYFFHIDDGIAVHDEEGTELEDVAVAKCEAVKLAGQMICESA
                     GTFWNRQEWKLTASDEIGLTLFCLHFVGVEAPASMSRRDPNMISARIAATPRAPAGGP
                     APDVDDAPTIQAG"
     gene            2350..2541
                     /locus_tag="VGB57_06910"
     CDS             2350..2541
                     /locus_tag="VGB57_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131117.1"
                     /translation="MSFKVMWMRGPLTMGSEAFDDLGTATRYATDQLPDMQSRFGATA
                     VKIVDGLGTPHFLRSLSRT"
     gene            2597..2803
                     /locus_tag="VGB57_06915"
     CDS             2597..2803
                     /locus_tag="VGB57_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131118.1"
                     /translation="MGKVYDTPSEVTVEDGNVAVEGPDGVDVLLTPEAALETSDRLLD
                     GATEAHGQRVRKAEAETERPSPTV"
     gene            3139..3483
                     /locus_tag="VGB57_06920"
     CDS             3139..3483
                     /locus_tag="VGB57_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0131119.1"
                     /translation="MGSREQTSLARLGAELERLADELVRLGGEAQSKEPPPSPGGERP
                     ADRLRRRLLEARRARGLRQSDLADKLGRPQSFVSNYERGERRLEVSEFIVIARVLGVD
                     AAAVVAELVEEG"
     gene            complement(3535..4326)
                     /locus_tag="VGB57_06925"
     CDS             complement(3535..4326)
                     /locus_tag="VGB57_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEY0131120.1"
                     /translation="MATQPPSNTLPIFYGGLEPLSSSVHGGYRSRSSDRAPFLTRNHA
                     VPITIDEFVSVQRYFPIVFSVGENPVPLALMGLNEGVNVFVDSDGKPTGDIYLPAYVR
                     RYPFMLARLQPEAQELSLCFDPTSGLIGDFEDGEPLFDDGKPSETTNNILKFCEEFEL
                     SAQRTVAFMTELKDSGLLMDGEVSIQPAGVDQPFVYRGFQMINEEKLRDLQGDDLKRM
                     NGNGMLPLILAHLFSVSLVREVFGKQMQQGKGPSVPGMAAPAGNA"
BASE COUNT          715 a         1519 c         1497 g          677 t
ORIGIN      
        1 cgcccctgaa ccggccgcgc ccgatgcgcg tccgcctcga ctttctatgc ctgaaggcgg
       61 agcgctgttc cagcgcgcgc gcggcggccg gctgcacctg ggcggcggaa gacgattgcg
      121 tgccaaatcc ggactactta ccttgcatct gcaaatgcgc gactccacgc cccctttccg
      181 ccgcctcggg ccggaaaagc cgagctcccc ggtgctcgtt tccgtgcccc atgccggccg
      241 ggcctattcc gacgggctgc tcgcctccgc ccggctctcg cgggctcggc tcgaaagcct
      301 ggaggaccgg ctcgtcgatc ggctcgtctg gcgcgcggtc gagggcggag cggcggctct
      361 gatcgccgac gcgccgcgcg ccgagattga tctcaaccgc gacgaacgcg agctcgatcc
      421 ggccatggtg cagccgcggc cggccgctca cctgacggtc gaatccccgc ggacccgcgg
      481 cggcctcggc ctcattccgg cccggatcgc gggatcggga gcgatctggc ggcagcgcat
      541 cgccgcggcc gaggtcgccc ggcgcgtgga gcatatccat cgcccttatc atgccgcgct
      601 ggaggcggaa ttgcgctcgg tcaaagcgcg cttcgggatc gccatcctgc tcgattgcca
      661 ctccatgcct ccgcgcggcg gcgagggcga ggccccggtc gtgctcggcg accgccacgg
      721 cgggagcata gccgaagccc tggtcgccgc cgcggagggc gcggtgcgcg cggccggctt
      781 caaggtcgcc cgcaacgcgc cttatgccgg cggccacatc acggagcggc acggccgccc
      841 cgcttgcgga ttccacgcgc tccagctcga gctcgaccgc tccctctatc tcgcccccga
      901 tctgcgcacg accggccccg ggttcgaccg ggtcgcccga ctcatcgccg ccatcgccga
      961 ggcgctcgcc gccgccgcgc tcgacccccc cgcggcgatc gcggcggaat aggatgcgcg
     1021 ggcgacttct cctccttgcg ccggcggtcc tgctcggcgc cgcgccggcc gcggcgcgcc
     1081 cgcccgtcga gactccgcgg ctcctctcca acggctggca ctacggcgag gccggcaatt
     1141 attgcatggc catccgtcag ctcgcgggcg gcgagcgcct gagcatgcgc ctcgccaagt
     1201 gggacgatca ctccgacagc ctgatcctct gggctgcggg cctcccgccc ctcgacctcg
     1261 acgacgaggc cgccgccgaa cgccattacg atctggaggt gcgcatcgac ggccggcgca
     1321 taccgcgcgc catgcattcg aacatgctcg aggaattcga cggcaagccc ggtccgagct
     1381 accggcttgg cattgcccag aagcccttca tcgccgcgct cgccaaagga cgcaggctgg
     1441 agatcgaacg ggccggcaag ctcgtccgcg ccttcccgat cgccggcagc gcggccatgg
     1501 cccgcctctt cgccgactgc gtcgagaaag acccgagctt ctgagcgaag cgattgccag
     1561 gcagcgcctg tcggcttagg atcggatcgc tccggctatg ggagaatggg catgatttcg
     1621 aagcggacct atctggccgc cgcggcgctg atcgcgctgg ccgcgttcgc gaccggccag
     1681 gcctttccgg gaatgggcgt gcccttcctc gtcctcgcgt cgaccctctg ggtcgcctgg
     1741 accgtccggc gcggaggcag gcgcggcctc cgataaaggg cggctccctc gttcgccgga
     1801 aatgagtctg gcggcggcgc caaaagagcg tatggaacca catcgtcgag ttcaccccag
     1861 gcaccggcgg ctgagagaca ttacgagctc ccgcctctgg taggaggacc ggcatgccca
     1921 gatatttttt ccacatcgac gacgggatcg ccgtgcacga tgaggaaggc accgagttgg
     1981 aggacgtggc ggtggccaaa tgcgaggccg tcaagctcgc cgggcagatg atctgcgaat
     2041 cggcgggtac gttctggaac cgtcaggagt ggaagctgac cgcctcggac gagattggcc
     2101 tgaccttgtt ctgcctccac ttcgtcggcg tggaggcgcc ggcttcgatg agccggcgcg
     2161 atcccaacat gatctcggcc cgaatcgcgg ccaccccccg ggcgccggcc ggcgggcctg
     2221 cccccgatgt ggacgacgcg cctacgatcc aggccggctg accgacctgt cgcgaagggc
     2281 gacctgccgg caggggtcgg ttaacgcctt tctgctagcg caaggcttcc accgcgcgaa
     2341 agactatcca tgagcttcaa agtcatgtgg atgaggggcc cgctgaccat gggcagcgag
     2401 gctttcgacg atctcggcac cgcgacccgc tacgcgaccg atcagcttcc cgacatgcag
     2461 agccgcttcg gcgccaccgc ggtgaagatc gtcgacgggc tcggcacccc gcattttctg
     2521 cggtcgctca gccgcaccta agcggccgga ccccgccccg ggggaaccag cggccgggcg
     2581 aaggcgctat tacgtcatgg gaaaagtata cgacacgccg agcgaagtga cggtggagga
     2641 tggcaatgtc gcggtcgaag gacccgacgg agtcgacgtg cttctgaccc cggaggccgc
     2701 gctggagacg tccgaccgcc ttctcgacgg cgccaccgag gcgcacggcc agcgggtcag
     2761 gaaggcggaa gccgagacgg agcgcccttc cccgaccgtc tagaaggagc gcccatgccg
     2821 tcgatccgcc tccggccgac ggccttccat atgaaaagac caccccgcga tgctcgcggg
     2881 gtggccaggt tcagggagaa gcgccccgaa gggcgcaacg ctccgccgca aagggggaaa
     2941 tacgtcggcg cgtctggcta aatgtatgaa agtcctggaa ggtttcaaga gctccggtcg
     3001 ccgcgccggg ccccgggcgc atcacgatca gggcggtcct cgcgacgttg cgcaatgcgt
     3061 ccgatgcggc ctcgacaacc caacgcaaag caactagtcc atgcgcgtgg atttcgctta
     3121 acgtggggaa gtgcgagcgt gggaagcagg gaacagacaa gcctggcgcg gctcggcgcc
     3181 gagctcgagc ggctggcgga tgagctcgtc cgcctcggcg gcgaggcgca gtcgaaagag
     3241 ccgccgccgt cgccgggtgg cgaacgtccc gccgaccggc ttcgccggcg ccttctcgaa
     3301 gcgcggcgag cgcgcggcct gcgccagtcg gacctcgccg acaagctggg acgcccgcaa
     3361 tccttcgtct ccaattacga gaggggcgag cggcgactcg aagtctccga gttcattgtc
     3421 atcgcccggg tcctgggcgt ggacgccgcc gcggtcgtcg ccgagctggt cgaggagggg
     3481 tgaaagcggt ccgccgtcat cccggcgaaa gccgggataa cggcgtggcc gcgctcaggc
     3541 gttgcccgcc ggcgccgcca tgcccgggac cgacggccct ttgccctgct gcatctgctt
     3601 gccgaagact tcgcgaacca gcgagacgga aaagagatgg gcgaggatca gcggaagcat
     3661 gccgttcccg ttcatccgct tcagatcgtc tccctggagg tcgcgcaatt tctcctcgtt
     3721 gatcatctgg aagccgcgat agacgaaggg ctgatcgact ccggccggct ggatcgagac
     3781 ttcgccgtcc atcagcaggc cggaatcctt caattcggtc atgaaggcga cggtgcgctg
     3841 ggccgaaagc tcgaactcct cgcagaattt caggatgttg ttggtggtct cgctcggctt
     3901 gccgtcgtcg aacagcggct cgccgtcctc gaaatcgccg atcaggccgc tggtggggtc
     3961 gaagcagagc gaaagctcct gagcttcggg ctggagccgg gcgagcatga acggatagcg
     4021 gcggacataa gcgggaagat agatgtcccc ggtcggcttg ccgtccgaat cgacgaagac
     4081 gttgacgccc tcgttcaggc ccatcagcgc cagcggcacc ggattctcgc cgaccgagaa
     4141 gacgatcgga aagtaacgct gcacggacac gaactcgtcg atcgtgatcg gcaccgcatg
     4201 gttgcgggtg aggaagggcg cgcgatccga cgagcggctg cgatagccgc catggacgct
     4261 gctcgacagc ggctcgaggc cgccatagaa aatcggcagg gtgttcgacg gcggctgggt
     4321 cgccatgttc atgtctccgc tagcgttgga ggcggccgcg tctcgggccg cccgaatgtg
     4381 gcggcggtta ctatggcgca atcgcgtc
//
LOCUS       DASWNU010000345         7516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_142244, whole genome shotgun sequence.
ACCESSION   DASWNU010000345 DASWNU010000000
VERSION     DASWNU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142244"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VGB57_06930"
     CDS             <1..619
                     /locus_tag="VGB57_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0131121.1"
                     /translation="SDSELHTARKAVGASGADIVTGAAFSDSAIKARGDLSNYRILHF
                     ATHGLVTAPRPECPARPALMTSFAGGDSDGLLSFAEIYDLRLDADLVILSACDTAGKA
                     SLAASREAGLRSRGEFALDGLVRAFVGAGGRSVIASHWPVPDDYDSTNRLISGLFEAP
                     PGTATATALRQAQEKLMQSPATSHPYYWAGFAIVGDGAAPVVPKR"
     gene            908..3040
                     /locus_tag="VGB57_06935"
     CDS             908..3040
                     /locus_tag="VGB57_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0131122.1"
                     /translation="MGAVAAGALALGTASPAFAQTASDASDPPQTPAADEPIVPDSQF
                     EEELPALDPELGRPLEPLEDFAGPPPATTPEGELIPDAAVPDPELERPLTPISEFDVT
                     TPPPTAGDGDDAEKVEPVRYTLVLEGFEEIGLDDRFRELSALEDADGEATNGAQISAR
                     AKEDELLAVRLLRSEGYYDATALSSIEQLPDQPGQMRVVVSAVPGPRYLFGNITIEGA
                     QTEPANLAREALPLKPEDPIVALNVEAAEANVRLRLPQQGYPFAEVGLRDILLDPETR
                     HGDYTLPVNPGNRSRFAGFTTEGDLAFGAKHVEVLSRFKRGELYDSRKVDDLREAMIA
                     TNLFTTVSAAPVATGEMAEDGTEYVNILVRQDAGPARSLAGTLGFSTGEGFRAEATWE
                     HRNLFPPEGGLMVNAIAGTQEQLLRVRLRRNNWKKRDRTIFVQAEAGRRDLPAFKGYT
                     TRLQAQVARESTPIWHKVWTYAVGAELIATNEAQTGFPPLSIADAFFLAGVSGQVGYD
                     RSNSLLDPTRGFRVKAFVNPETSLREPGQPYIRNILDGSVYYPAKENIVIAGRARVGS
                     IYGARLNELAPSRRLYAGGGGSVRGFGFQELGPKIEVANPDFDPTDPDEKDDPTRLIP
                     TGGRSMVEFAVEARYRFGNYGVVGFVDAGQVYESQSPRLNDMRFGVGVGGRLYTNFGP
                     LRADIAVPLGRRRGESKFAVYVSIGQAF"
     gene            3040..7263
                     /locus_tag="VGB57_06940"
     CDS             3040..7263
                     /locus_tag="VGB57_06940"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0131123.1"
                     /translation="MATAAEPAPRSHRGRQRARAAAKWTGVGLIGLVVAILLFFVWLN
                     SSLGHRFVVKQINQLETASGLDIDVGRIEGSLFGELTLHNLTLKDPKGTFFLAPRATL
                     DWRPLAYFRNHIDIKRLEVPQARLYRLPELKPGDPNAPLLPDINVDIGQIKVDRMLID
                     PPVTGYRHLLSLAGSARIDDGRAQVALESNAIAGPGLPGGDKLALRIDAVPEANRLAM
                     GVRVEAPANGFVSKLTGINQPLVATVGGNGTWTNWRGRARAILGGKQFADLAVGAKDG
                     TFTVAGPMNPGLLMAAGIPRNLLSLQVQVNLTTRLDQRRADTRLSLTSPAIAVVADGM
                     LDLGRSQFQDFKVAARLSRPRVIGPNINGTDLRVAMVLNGAFRTPFVAYDLRARSLSF
                     NETTIELLQARGRAKVDTDRISIPIQARARRISGLDPSLGSLLTNVGVNGTINISGTK
                     LVSDNLKIRSDRLNATVVLAADLAKGQYRAGIQGTVNNYLMEGIGLLDLTTNMNVFTA
                     GNGFGLQGRVSVRTRRIDNPTAAGVLEGNATASANVVMNPAGVITLDDVRLSSPGLRI
                     TSGGGTVWPDGRINIRAAGVSRQYGAFAVVVTGTATRPQIQLRAASPGFGIGLRDITA
                     TVRAVAGGYAVSARGQSNYGPFEADVTILSGRGPLTIDVRRLLFAGMTFTGRVSQSAA
                     GPFVGTLAMTGQGLQGTIQLAAVGRYQRADVNATANGAQIPGANPILIQRGIIQASVI
                     LYDDAPHIVGDAQLAGVRASNFFLARARAKVDYRGGNGLAQVFAEGTSGMPFRVAVNA
                     ALSPNLIRAAAQGQVRNIPFRLARPAQISKVAGGWQLAPTELVLPQGNVQLAGRYGAG
                     GMVVQSRLNDFDISILNVFSPGMGVGGRATGSIDFAQVGGDTFPRADARLAIAGFTRT
                     GIAVRSPPVDLALAGQLRPEGGSLAAVIRRDAAVIGRAQLRLQPVGPGAGGWSTRLLA
                     APLAGGIRYNGPAEVLWSLGGVADMQLSGPIGVAADFSGRVQSPQLAGIVRANNLTFV
                     HETYGTRISQLALQGRFTSSEFQITRLSGRAGRGTISGTGTVGFAAAAGYPMDIRLRL
                     ADAQLARSDNIGATLTGDLAITNSRANGALISGDLTLPEVRYQIIRQGAAQVVELAGV
                     RRKGEPLPTVRDATQQADNTAPSIWKLDLRLRADNQLFIAGMGLESEWSADLRVQGTT
                     ATPSIVGEADLIRGTYSFSGQRFDVTRGHIAFTGARPPNPRLDIVASADIKDVTVNIN
                     VSGSASNPQIAFTSNPGLPQDEIMARILFGGSVTEISALQAVQLATSLNSLRGGGGGL
                     NPLGKLRSSTGLDRLRILGADDTTGRGTAVAAGFYISNDIYLEIITDARGFTATQIEI
                     ALSRALSILSQVGTSGGSNNVNLRYRKQY"
BASE COUNT         1192 a         2790 c         2408 g         1126 t
ORIGIN      
        1 ctcggattcc gagcttcaca ccgcgcgcaa ggcggtcggg gcgagcggcg ccgacatcgt
       61 caccggcgcg gccttcagcg acagcgcgat caaggcgcgc ggcgacctct ccaactaccg
      121 catcctccac ttcgccaccc acgggctggt gacggcgccc cgccccgaat gcccggcccg
      181 gccggcgctg atgacctcgt tcgcgggcgg cgattcggac gggctgctca gcttcgccga
      241 aatctacgac ctgcgcctcg acgccgatct cgtcatcctc tccgcctgcg acaccgccgg
      301 caaggcgagc ctcgccgcca gccgcgaggc cgggcttcgc agtcgcggcg aattcgctct
      361 cgacgggctg gtgcgcgcct tcgtcggagc gggcgggcgc tcggtcatcg ccagccactg
      421 gccggtgccc gacgattatg actcgaccaa ccggctcatc tcggggctgt tcgaggcgcc
      481 ccccggcacc gcgaccgcga ccgcgctgcg ccaggctcag gaaaagctga tgcagtcgcc
      541 cgccacctcg catccttatt attgggcggg cttcgcgatc gtcggcgacg gcgccgcgcc
      601 ggtggtgccg aagcggtgac gggatgacgg ggtgacgggg cggccgtacg gcccctgccg
      661 tcattctgaa cttgtttcag catccagggt cgaaccgcct cgcgggcgga ggactggacc
      721 ctgaaacaag ttcagggtga cggcgatgcg cgatgcacag cccaatctat gacgtggccg
      781 acaggccaca cgaactctcc cttacgcctc cggaacaaat gcaagctttt ccagtggaac
      841 gtttgtcggc ttaaggaccg ggtttgacct cgggagcggc gggcatttga agaaggccgg
      901 tattttgatg ggagcggtcg ctgccggtgc cttggcgctg ggcacggcct cgccggcatt
      961 cgcccagacc gcctccgacg cctccgatcc gccccagacc ccggccgccg acgagcccat
     1021 cgttcccgac agccagttcg aggaggagct gccggcgctc gatccggagc tgggcaggcc
     1081 gctcgagccg ctcgaggatt tcgccggacc gccgcccgcc acgacgcccg agggcgagct
     1141 gatccccgac gcggccgtgc ccgaccccga gctggagcgg ccgctcacgc ccatctccga
     1201 attcgacgtc acgaccccgc cgccgacggc gggcgacggc gacgacgccg agaaggtcga
     1261 gccggtccgc tacacccttg tcctcgaagg gttcgaggag atcgggctgg acgaccgctt
     1321 ccgcgagctt tccgcgctcg aggatgccga cggcgaggcg accaacggcg cccagatctc
     1381 cgcccgggcg aaggaggacg agcttctcgc cgtccgcctg ctccgctccg aaggttatta
     1441 cgacgcgacc gccttgtcct cgatcgagca attgcccgat cagcccggac agatgcgggt
     1501 ggtggtgagc gcggttccgg gcccgcgcta cctgttcggc aacatcacga tcgagggcgc
     1561 ccagaccgag ccggccaacc tcgcccggga ggccctgccg ctcaagccgg aggatccgat
     1621 cgtcgccctc aacgtggagg cggcggaagc caatgtccgg ctgcgccttc cgcagcaggg
     1681 ctatccgttc gccgaggtcg gccttcgcga catccttctc gatcccgaga cccgccacgg
     1741 cgattacacc ctcccggtca accccggaaa ccgctcccgc ttcgccggct tcacgaccga
     1801 aggcgacctc gctttcggtg cgaagcatgt cgaggtcctc tcccgcttca agcgcggcga
     1861 gctctacgac agccgcaagg tggacgacct tcgcgaggcg atgatcgcga ccaacctctt
     1921 caccaccgtc tccgccgctc cggtcgcgac gggggagatg gccgaggacg gcaccgaata
     1981 tgtgaacatc ctcgtccgcc aggacgccgg gccggcgcgg tccctggcgg ggacgctcgg
     2041 cttttcgacc ggcgagggtt tccgggcgga agccacctgg gagcatcgca acctgttccc
     2101 gccggaaggc gggctgatgg tcaacgccat cgccggcacc caggaacagc tgcttcgggt
     2161 ccgcctgcgc cgcaacaact ggaagaagag agaccggacc atcttcgtcc aggccgaggc
     2221 cgggcgccgc gacctgcccg ccttcaaggg ctatacgaca aggcttcagg cgcaggtcgc
     2281 ccgcgaatcc actccgatct ggcacaaagt gtggacctat gccgtcggcg ccgagctgat
     2341 cgcgaccaac gaggcgcaga cgggattccc gcccctctcc atcgctgacg ccttcttcct
     2401 cgcgggcgtc agcgggcagg tcggctacga ccgctcgaac agccttctcg atccgacccg
     2461 gggcttccgc gtcaaggcct tcgtcaaccc ggagacgtcg cttcgcgagc cgggccagcc
     2521 ctatatccgc aacatcctcg acggcagcgt ctattatccg gcgaaggaga atatcgtcat
     2581 cgccggccgc gcccgggtcg gctcgatcta cggcgcccgg ctgaacgagc tggcgccgtc
     2641 gcggcggctc tatgcgggcg gcggcggatc ggtgcgcggc ttcggcttcc aggagctcgg
     2701 gccgaaaatc gaagtggcga accccgattt cgatccgacc gatcccgacg agaaggacga
     2761 tccgaccagg ctgatcccga ccggcgggcg aagcatggtc gagttcgcgg tcgaggcccg
     2821 ctaccgcttc ggcaattacg gagtggtcgg gttcgtcgac gccggccagg tctatgaaag
     2881 ccagtctccc cggctgaacg acatgcgctt cggagtcgga gtcggcggcc gcctctacac
     2941 caatttcggg ccgttgaggg cggacatcgc cgttccgctc ggccgccgcc gcggcgaatc
     3001 gaaattcgcc gtctacgtct cgatcgggca ggctttctga tggcgaccgc ggccgagccg
     3061 gcaccccgtt cgcaccgcgg ccgccagcgc gcccgcgccg ccgccaaatg gaccggcgtc
     3121 ggcctgatcg gcctcgtcgt cgccatcctc ctcttcttcg tctggctgaa ctcgtcgctc
     3181 ggccaccgct tcgtcgtgaa gcagatcaac cagctcgaga cggcgtccgg gttggacatc
     3241 gacgtcggcc ggatcgaagg ctccctgttc ggcgagctca ccctccacaa tctgaccctg
     3301 aaggacccga aggggacctt cttcctcgcc ccccgcgcga ctctggactg gcggccgctc
     3361 gcctatttcc gcaatcatat cgacatcaag cggctcgaag tcccgcaggc gcgcctctac
     3421 cgcctgcctg agctcaagcc gggcgacccc aacgccccgc ttctccccga catcaacgtc
     3481 gatatcggcc agatcaaggt cgaccggatg ctgatcgacc cgccggtgac cggctatcgc
     3541 cacctcctca gcctcgccgg aagcgcccgc atcgacgacg gccgcgccca ggtggcgctc
     3601 gaatccaatg cgatcgccgg gcccgggctg cccggaggcg acaagctcgc cctgcgcatc
     3661 gatgccgtgc cggaggccaa ccgcctggcc atgggagtcc gggtcgaagc gcccgccaac
     3721 ggcttcgtct ccaagctgac cggaatcaac cagccgctgg tcgcgaccgt gggcggcaac
     3781 ggcacctgga cgaactggcg cggccgggcg agggccatcc tcggcggcaa gcaattcgcc
     3841 gacctcgccg tcggcgccaa agacggcacc ttcaccgtcg ccggaccgat gaacccgggc
     3901 ctgctgatgg cggcgggcat tcccaggaac ctgctgtcgc ttcaggtcca ggtcaatctc
     3961 acgaccaggc tggaccagcg ccgggccgat acgaggctga gcctgacctc cccggcgata
     4021 gccgtggtgg cggacgggat gctcgatctc ggccggagcc agttccagga tttcaaggtc
     4081 gccgcgcgcc tttcgcgccc gcgggtgatc ggccccaaca taaacggcac cgacctgcgc
     4141 gtggcgatgg tcctgaacgg cgccttccgg acgccgttcg tcgcctacga ccttcgcgcc
     4201 cgcagcctga gcttcaacga gacgacgatc gagctcctcc aggcgcgcgg ccgggccaag
     4261 gtcgacacgg atcggatcag catccccata caggcgcgcg cccgccggat cagcggcctc
     4321 gatccgtcgc ttggcagcct gctcaccaac gtcggggtga acggcacgat caacatcagc
     4381 ggcacgaagc tcgtctccga caacctcaag atccgctccg atcgcctcaa cgccaccgtc
     4441 gtcctggccg ccgacctcgc caagggccag tatcgcgccg gcatccaggg gacggtgaac
     4501 aattatctca tggaggggat cggcctcctc gacctcacca cgaacatgaa cgtgttcacc
     4561 gccggaaacg gcttcggcct tcagggccgg gtctcggtcc ggacccggcg gatcgacaat
     4621 ccgaccgccg ccggagtgct cgaaggcaat gccaccgcct cggccaacgt ggtgatgaat
     4681 ccggccggag tgatcacgct cgacgacgtc cggctgagct cgccggggct tcggatcact
     4741 tcggggggcg gaacggtctg gcccgacgga cggatcaaca tccgggcggc cggggtctcg
     4801 aggcaatatg gcgccttcgc ggtcgtcgtg acggggaccg cgacaaggcc ccagatccag
     4861 cttcgagcgg caagtccggg cttcggaatc ggacttcgcg acatcaccgc gacggtgcgc
     4921 gccgtcgccg gcggctatgc ggtgagcgcg agggggcaat cgaattacgg gccgttcgaa
     4981 gccgacgtca ccatcctgtc cgggcgagga ccgctgacca tcgacgtccg ccgactgctc
     5041 ttcgccggaa tgaccttcac cggccgggtc agccagtcgg ccgccgggcc cttcgtcgga
     5101 accctcgcga tgaccggcca gggcctccag ggcacgatcc agctcgccgc cgtcgggcgc
     5161 taccagcgcg ccgacgtcaa cgcgacggcc aacggcgccc agattccggg cgctaacccg
     5221 atcctgatcc agcgcgggat catccaggcc agcgtgatcc tttacgacga cgcgccgcac
     5281 atcgtcggcg acgcccagct cgccggggtg cgcgccagca acttcttcct cgccagggcg
     5341 cgggccaagg tggattatcg cggcggcaac ggcctcgccc aggtcttcgc ggaagggacg
     5401 agcggaatgc ccttccgggt ggcggtcaac gcggcgctgt cgcccaacct gatccgcgcc
     5461 gcggcccagg gccaggtgcg caacatcccc ttccgcctcg cccgtccggc gcagatcagc
     5521 aaggtggcgg gcggctggca gctcgcgccc accgagctgg tcctgccgca gggcaacgtc
     5581 cagctggcgg gccgctacgg cgccggcggg atggtggtcc agtcgaggct caacgacttc
     5641 gacatctcga tcctcaatgt cttctcgccc ggcatgggcg tcggcggccg ggccaccgga
     5701 agcatcgact tcgcccaggt cggcggcgac accttcccgc gcgccgacgc gcgcctcgcc
     5761 atcgccggct tcacccgcac cggaatcgcc gtccgctcgc cgcctgtcga cctcgctttg
     5821 gcggggcagc tccggcccga gggcggcagc ctcgccgccg tgatccgccg cgacgccgcc
     5881 gtcatcggcc gggcccagct tcggctccag ccggtcggcc ccggcgcggg cggctggtcg
     5941 acccggctgc tcgccgctcc gctcgccggc ggcattcgct ataacggtcc cgccgaggtc
     6001 ctctggtcgc tgggcggagt cgccgacatg cagctctccg gcccgatcgg ggtcgccgcc
     6061 gatttctcgg gccgggtgca gagcccgcaa ctcgccggaa tcgtccgggc caacaatctg
     6121 accttcgtcc acgagaccta cggaacgagg atcagccagc tggccctgca aggccgcttc
     6181 accagctccg agttccagat cacccggctt tccggccgcg ccggccgcgg cacgatcagc
     6241 ggtaccggca cggtcggctt cgccgcggca gccggctatc cgatggacat ccgcctcagg
     6301 ctcgccgacg cccagctcgc ccgaagcgac aatatcggag cgaccctgac cggcgatctc
     6361 gccatcacca acagccgcgc caacggcgcc ctcatctcgg gcgaccttac cctgccggag
     6421 gtgcgctacc agatcatccg ccagggcgcc gcccaggtgg tcgagctcgc cggggtgcgc
     6481 cgcaagggcg agccgcttcc gaccgtaagg gacgcgaccc agcaggccga caacacggcg
     6541 ccgagcatct ggaagctcga cctgcgcctt cgcgccgaca accaattgtt catcgccggg
     6601 atgggcctcg aatccgaatg gagcgcggac ctcagggtcc agggcacgac cgcgaccccg
     6661 tcgatcgtcg gcgaggccga cctgatccgc ggcacctaca gcttctcggg ccagcgtttc
     6721 gacgtcaccc gcggccatat cgccttcacc ggcgcccggc cgcccaatcc gcgcctggac
     6781 atcgtcgcga gcgccgacat caaggacgtc accgtcaaca tcaacgtctc cggcagcgcc
     6841 agcaacccgc agatcgcctt cacctccaac ccgggcctgc cccaggacga gatcatggcc
     6901 cggatcctgt tcggcggctc ggtgacggag atttccgcgc tccaggcggt gcagctcgcc
     6961 acatcgctca actcgctgcg cggcggcggc ggcggcctga acccgctcgg caagcttcgc
     7021 tcctcgaccg gcctcgaccg ccttcggatc ctcggcgccg acgacacgac cggccggggc
     7081 accgccgtcg ccgccggctt ctacatctcc aacgacatct atctggagat catcaccgac
     7141 gcccgcggct tcaccgccac ccagatcgag atcgccctgt ccagggcgct gagcatcctc
     7201 tcccaggtcg ggacgtcggg cggaagcaac aacgtcaacc tccggtaccg caagcaatat
     7261 tgagctgaat ccctcccttg cctttggttg aggtcgcggc ggggacggtc gcgccttggc
     7321 tgcccttcgg cgccattacc cgcctgccgc tggttcaggc ggtccgatcg gcggctcggg
     7381 caagtttcca ggcgacgagg gtcaggaagg cgacggcggt attccgtccg gcacccctgt
     7441 agcgcacccg ggctgggcca tgggaacgcc tgggctgcat cgaaattcgg ctaaggtcgc
     7501 tccctcttaa gtcctc
//
LOCUS       DASWNU010000346         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_91084, whole genome shotgun sequence.
ACCESSION   DASWNU010000346 DASWNU010000000
VERSION     DASWNU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91084"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VGB57_06945"
     CDS             <1..607
                     /locus_tag="VGB57_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0131124.1"
                     /translation="RAEGLLKDNGLGDYVDKPIRSLSKGMAQTVQLFGTIVHRPKLIV
                     LDEPFSGLDAINQGRLENLIRDEAAGGATIIFSTHVIAHAERLCERIAIIAGGKVRFE
                     GSVAEARDRLRPQVRLRTRVADGPWRGALPPDAHHEDGLWAFELPQVGIEPLLKALID
                     AGAGIEDLSIERPGLHDAFVAIAGEQAARELADSAAEGEAR"
     gene            604..1827
                     /locus_tag="VGB57_06950"
     CDS             604..1827
                     /locus_tag="VGB57_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0131125.1"
                     /translation="MMGFFQSAYVIARRDFVATVWSRTFLFFLLGPLLIIGLSFLFGN
                     MSGRMARQDVHATVAVVAAEADFAALAAARERLSPGFAARPLPTLVREAPGGEPSRQV
                     DTLLSNPDKRILAVMTGPLDRPVLTGAITSEGSIRKQMSLIVDQARQQVALAVAGRPT
                     PPAAIEVVETDRSEGALAATRAITARGGQLLLFMLTVLLAGMLLSNLLEEKSNKIIEV
                     LAAALPIDAIFLGKLVSMLAISLVGIAVWVGGAVIALALWPGQAPGLPEPAVGWPLFI
                     LLVLLYYSANYLLLGALFLGIGSQANSVREVQTLSMPVTVGQMLIFLLASFAVGAFNS
                     PLGLGAAIFPFSSPLTMVARAAQTSELWPHLLALGWQALWVWLTVALGAKLFRRNVLK
                     SGGGQGGMLRRRKSA"
     gene            complement(1959..2330)
                     /locus_tag="VGB57_06955"
     CDS             complement(1959..2330)
                     /locus_tag="VGB57_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0131126.1"
                     /translation="MAKVTGLGGVFYKVEDSEATARWYKETLGVGGQWGATFPFAGDP
                     GGYTLLSPFKSSSDYFAPSEAAFMINLRVDDLDAMIAQLEAKGIDILGRQDEDYGKFA
                     WILDCDGIKVELWQQVGPAPE"
     gene            complement(2323..3081)
                     /locus_tag="VGB57_06960"
     CDS             complement(2323..3081)
                     /locus_tag="VGB57_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY0131127.1"
                     /translation="MATSQDKARLFASLHRPGDPLVLFNAWDPGSAKTVAEAGAKAIA
                     TGSASVAAAHGFGDAEALALELALANAARVVRSVELPVTIDFEGAYGTEPEAVAANMK
                     RLAETGAVGCNFEDQVIGGEGLHPVEAQAERIRAARAAVGPDFFINARTDIFLKTKAA
                     THEIAAIEDALVRVRAYAEAGASGFFVPGLVDLHQLAQICVASPLPVNFMAFPGAPAA
                     PAVAAAGVARISHGPYPYLLVMKTLKQAAETIYG"
BASE COUNT          487 a         1142 c         1057 g          536 t
ORIGIN      
        1 ccgcgccgag gggctgctca aggataatgg cctcggcgac tatgtggaca agccgatccg
       61 ctccctctcc aagggcatgg cgcagaccgt ccaactgttc ggcaccatcg tccaccggcc
      121 caagctcatc gtcctggacg agcccttctc cggcctcgac gcgatcaacc agggccggct
      181 cgagaatctc atccgggacg aagcggcggg cggagcgacg atcatcttct cgacccacgt
      241 catcgcccat gccgagcgcc tgtgcgagcg gatcgcgatc atcgccggcg gcaaggtccg
      301 cttcgaagga agcgtcgcgg aagcgcgcga ccggctccgg ccgcaggtgc ggcttcgaac
      361 ccgggtcgcc gacggcccct ggcgcggcgc gcttccgccg gacgctcatc acgaggacgg
      421 cctctgggcg ttcgagcttc cccaggtggg gatcgagccg ctgctcaagg cgctgatcga
      481 cgccggcgcc ggaatcgagg atctgtcgat cgagcggccg ggcctgcacg acgccttcgt
      541 cgcaatcgcc ggcgagcagg cggcgcgcga gctcgccgac agcgccgcgg aaggagaggc
      601 gcgatgatgg gtttcttcca atccgcctac gtcatcgccc gccgcgactt cgtcgcgacg
      661 gtgtggtccc ggaccttcct cttcttcctg ctcgggccgc tgctcatcat cggcctgtcc
      721 ttcctgttcg gcaacatgag cgggcggatg gcgcgccagg acgtccatgc gaccgtggcc
      781 gtggtcgcgg cggaagcgga tttcgccgcc ctcgcggccg cccgcgagcg gctcagcccc
      841 ggcttcgcgg cgcggcccct gccgacgctc gtccgcgagg cacccggcgg cgagccgtcg
      901 aggcaggtcg acaccctgct gagcaatccc gacaagcgga tcctcgcggt catgaccggc
      961 cccctcgacc ggccggtcct caccggcgcc atcaccagcg agggctccat ccggaagcag
     1021 atgtcgctga tcgtcgatca ggcgcgccag caggtcgcgc tcgccgtggc gggccggccc
     1081 acgcctccgg ccgcgatcga ggtggtcgag accgaccggt cggaaggcgc gctcgcggcg
     1141 acccgggcga tcaccgcccg cggcggccag cttctcctct tcatgctgac cgtgcttctg
     1201 gccgggatgc tgctttccaa cctgctcgag gagaaatcga acaagatcat cgaagtgctc
     1261 gccgcggccc ttccgatcga cgcgatcttc ctaggcaagc tggtgtcgat gctggccatt
     1321 tccctggtcg gaatcgccgt ctgggtcggc ggcgccgtca tcgccctggc gctgtggccc
     1381 ggccaggcgc ccggcctgcc cgagcccgcc gtcggctggc cgctcttcat ccttctcgtc
     1441 ctcctctatt acagcgccaa ttacctcctc ctcggggcgc tgttcctcgg catcggcagc
     1501 caggccaatt cggtccgcga ggtgcagacg ctgtcgatgc cggtcacggt cgggcagatg
     1561 ctgatcttcc ttctcgcgtc cttcgccgtc ggcgccttca acagcccgct cggcctcggc
     1621 gccgccatct tcccgttcag ctcgccgctg accatggtgg cccgggccgc ccagacgagc
     1681 gaactgtggc cccatctgct ggcgctgggc tggcaggctc tgtgggtgtg gctgacggtg
     1741 gctttgggcg ccaagctgtt ccggaggaac gtgctcaagt cgggcggagg ccagggcggg
     1801 atgctgaggc ggcgaaaatc tgcctaaaat cctcccctgc atttgcaggg gagggggacc
     1861 acccgaaggg tggtggaggg gcctcactga cttggaagaa cccctccacc atgcttcgca
     1921 tggtccccct ccccgcgaaa tcgcggggag gatttaaatc actccggcgc cggccccacc
     1981 tgttgccaca gctccacctt gatcccgtcg caatcgagga tccaggcgaa cttgccgtag
     2041 tcctcgtcct ggcggccgag gatatcgatc cccttggcct ccagctgcgc gatcatcgcg
     2101 tcgagatcgt ccaccctgag gttgatcatg aacgccgctt cgctgggcgc gaaatagtcg
     2161 ctggatgatt tgaacgggga cagcaacgta tagccgccgg gatcgccggc gaacgggaag
     2221 gtcgctcccc actggccgcc gactccgagc gtctccttgt accagcgcgc cgtcgcctcg
     2281 ctgtcctcga ccttgtagaa aaccccgccg agccccgtca ccttagccat agatcgtctc
     2341 cgccgcctgt ttcagggtct tcatgacgag caaatagggg taagggccat ggctgatccg
     2401 ggcgacaccc gccgcggcca ccgccggcgc ggcgggggcg ccgggaaagg ccatgaaatt
     2461 gaccggcagc ggcgaggcca cgcagatctg cgccagctga tggagatcga ccaggcccgg
     2521 cacgaagaag ccgctcgcac cggcctcggc ataagccctg acccggacca gagcgtcctc
     2581 gatcgccgct atttcgtggg ttgccgcctt ggtcttgagg aagatgtcgg tgcgggcgtt
     2641 gatgaagaaa tccggcccga ccgcggcgcg cgcggcccgg atcctttcgg cctgcgcctc
     2701 gaccgggtgg agaccctcgc cgccgatgac ctgatcctcg aaattgcagc cgacggcgcc
     2761 ggtttcggcg agccgcttca tattcgcggc cacggcctcc ggttcggtgc cataagcgcc
     2821 ttcgaagtcg atcgtgaccg gaagctcgac ggagcgcacc acccgcgccg cattggccag
     2881 agcgagctcg agcgcgagcg cctcggcgtc cccgaaaccg tgcgccgccg caaccgaggc
     2941 gctcccggtg gcgatcgcct tcgcgcccgc ctcggccacc gtcttcgcgc tgcccgggtc
     3001 ccaggcgttg aagaggacga gcgggtcgcc aggccggtgc agcgaagcga agaggcgggc
     3061 cttgtcttga gatgttgcca tggcaaggaa gctacgcgga gcgcctgccg ctgtccatcg
     3121 gcccgccgcg catgcctggc gggagcggac cttgccaaac ggacgcgccc ggtgcctatt
     3181 atccgaatcg tggatatttg ggggtaatca tgactgatcc ga
//
LOCUS       DASWNU010000347         9724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_117911, whole genome shotgun sequence.
ACCESSION   DASWNU010000347 DASWNU010000000
VERSION     DASWNU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117911"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VGB57_06965"
     CDS             complement(<1..253)
                     /locus_tag="VGB57_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131128.1"
                     /translation="MIPRVLIDSAPIPGGEGELRLIQRGEDFWIMLGTNALMSTRLRG
                     SEEALATLACERIVGRPRPVMLIGGLGMGFTLRAALGALG"
     gene            332..799
                     /locus_tag="VGB57_06970"
     CDS             332..799
                     /locus_tag="VGB57_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY0131129.1"
                     /translation="MSVAFRRESDEEHKEPRFELPIPVGPNLVTANGLAGIEARVVQL
                     EASVAAETEEEPLAIAKRELRYWRTRLATAQLAPPPPGDEVAFGSRVAFRQNGALRSI
                     DIVGDDEADPSAGRISFSAPLARALIGACVGDRLDFAGRPEALEVVEIGPIPD"
     gene            complement(929..1654)
                     /locus_tag="VGB57_06975"
     CDS             complement(929..1654)
                     /locus_tag="VGB57_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234978.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0131130.1"
                     /translation="MRDLFSALGLALALGACSKAAPDPRGVARDEVLVQVSASGRADT
                     RPDEARFTVGVDTIAATSGEASARNSATINKVAAALQRLGVKEDDLQTRSIALARIDY
                     GPNRGRFQANNLIEIKVRDIRRAGEALAAITEAGANVLSGPNLRVSDPENAGRSAYAA
                     AYRAARGRAEAYAQAAGLKVARVLTITDAGIASPYRHYDGDAVMMEQAAAPPPVVATS
                     APPVQAGISTREVQIRVDFALSK"
     gene            1763..2407
                     /locus_tag="VGB57_06980"
     CDS             1763..2407
                     /locus_tag="VGB57_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEY0131131.1"
                     /translation="MQQLIRFAAFALAALLVVLMASPSAAQERAEGQSKADYLAWLSR
                     DAGARAQVLSFKAFLEAGDVEDVVPTWQLVRTASKWRDCGGPRFEVAPVAEWANISAT
                     LDFIKAEVEPVIGDVQPVSGFRNAELNQCSGGAKASAHRHFYALDLVPADEDVSRAAL
                     IRDVCRIHGNSGREFGIGLGFYSGMRFHLDSKGYRRWGANGSGATSPCATGNYA"
     gene            2750..6808
                     /locus_tag="VGB57_06985"
     CDS             2750..6808
                     /locus_tag="VGB57_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0131132.1"
                     /translation="MNRTYDVVAIQMTIWFNVEGDHDHNGLIYVLRRNLPLVKYLREL
                     AKPIPEQAELDRLRDAAKEIASEFQVPFPTTSEEAKKPHEFVRPLVLRAHHGDVVTVN
                     LENEIQGRAIGMHLVGPGYDVLKHDGSDVGANPPSLAPPGGKYSYVWTAQNQGVFPFH
                     DGGDYSGGQDGTNVHGLFGAFIVEPVGTIWRDPVSGQDSSVEGVQLDGLYLDIIPPEA
                     LIARPLPVPPTIENYQFPAPVEYTDYSREAHREFVVFFHDEPEFVPPHGTHYPDPCNP
                     DGGGGHGGHGGDHVPPIMPVSYRAEPMVNKEQILFRLLAQGHDFKGRPVLNEEQHHSS
                     WMFGDPATPIFKAYIGDPVRIRFVHAAVKETHVFHLHLYEWHAAPEDRKSPRIDAISV
                     SPQTGHTIEPVWGAGNRHQVAGDVIWHCHLYPHFHEGMWGMFRTFETLQEGSDGPLLA
                     QQDFPYTGRRIGRYPNDTRIERLLPLPGRVPPPRPTPKHPGYPLYIPGEMQQKSPIPP
                     WPMSTAMPVEYDYRQVPTGLERNAFNPQPVPGEIFTRRPFALGQSQEWPEGQTMPADK
                     PAYHLNNARMVRHNLTVNMARIDYNGHGWHDKHGHFYSLADGLKATSDQTLIRPPKEP
                     LFFRARHGQILNLTLTNQLPLEIPETEFDHAFPPCPARPWQGECTPHVHMVKFDPICA
                     DGASVGWNYISGPVAGKTMAYRWWLDQEFGTIFFHDHLFANFRQKHGLFGALIVEPAG
                     ARFLHNREEREIVSGAQARIDVPGATPKSFREFCMGLQDFIPMWDKNGEALNPPPAPG
                     GHGDQGIMAINYRNDPIHERLKDGSGAFIDPSLWFTSGPPYNRDPYTPRFDTLVTDPI
                     WFRVVQGSHEEQHSFQIHGMRWRRFRDNPASPLRNQQSTGISEAFTFKQEERYRAGDY
                     MYKLSGADDLWLGCWGVVRAFNYDDAERPAALTNRVPLGTDVMSSAVRTFHITAERRR
                     LVYRDPELVDPFGLIYRLTRIVGPDGRDIPVRQQQTPGREEPLILRCRRNEMVTIVLT
                     NGIPADADMRPEPFAPSVPVEEFDGQYRPVRPVSRHVSMHADLVRYDPTNSDGATVGR
                     NPRQSAAPGQTVTYYWVPHPADLPESGEPIGPVLLQDMADFRNHRHHGLIGALIVEPA
                     DARPLYVPEGKNTGDPVLASDTVDDARPGEAWDGPRATVIATVNGRQTKFEEAVLLLQ
                     DGLRHFIRGNLTLPVSDEPPTPGEDGVDHEDQGQKGFNYRSEPIERYLDNPNPATPVF
                     RVPENSSVRLHLVGAGDKPRQHSFTVHGVAWPEHRYMPKISPRVASESAISNGTARTF
                     EFNVGAPADYAYRSGVLKWAVPQGLWGLFRVG"
     gene            7447..7785
                     /locus_tag="VGB57_06990"
     CDS             7447..7785
                     /locus_tag="VGB57_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131133.1"
                     /translation="MKFFVPYAADDAHAEQIWSDARADLFDLGLPTTRRRIWALSLDV
                     RNPGHLLHIGGEVPDDYGPAMLIFEASNLDVFYVCTPWNGVLGGEPFVLGLGELGRAI
                     DFEEVVEGNA"
     gene            complement(7841..8179)
                     /locus_tag="VGB57_06995"
     CDS             complement(7841..8179)
                     /locus_tag="VGB57_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131134.1"
                     /translation="MRRSILLLLALGALPLGGCAAGLAASAVGAAVRAADRPDRAPRE
                     DPGPAARAACSARAAQYGTVRIIDTVYRSPAKLVVWGSVEGEGRKRSLQCRWDGKIVG
                     FEVKEVEERR"
     gene            8516..>9724
                     /gene="lepA"
                     /locus_tag="VGB57_07000"
     CDS             8516..>9724
                     /gene="lepA"
                     /locus_tag="VGB57_07000"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0131135.1"
                     /translation="MTDLNKIRNFSIIAHIDHGKSTLADRLIQSTGGLTEREMTSQVL
                     DNMEIEQERGITIKAQTVRLDYRAKDGEHYVLNLMDTPGHVDFAYEVSRSLAACEGAL
                     LVVDAAQGVEAQTLANVYQSIEHDHEIVPVINKIDLPAADVDKVRKEIEDIVGLPADD
                     AVLTSAKTGIGIAEVLEAIVTRIPPPKGDRDAPLKAMLVDSWYDPYLGVVILVRVIDG
                     SIRKGQNVSFMAAGTTHLVDRVGAFRPKIEQLPELGPGEIGFITAQIKDITETRVGDT
                     ITDARRPAAEALPGFKLVQPVVFCGLFPVDAADFEKLRESISKLRLNDASFSFEMETS
                     AALGFGFRCGFLGLLHLEIIQERLTREYDLDLITTAPSVVYLVHVSKSKDATSTGSAR
                     TVGTDEAGLKV"
BASE COUNT         1671 a         3369 c         3137 g         1547 t
ORIGIN      
        1 gcccgagcgc gcccagcgcc gcacgcaagg tgaagcccat cccgaggccg ccgatcagca
       61 tgacgggccg gggccgcccg acgatccgct cgcaggccag ggtggccagc gcctcctccg
      121 agccgcgaag gcgggtgctc atcaaagcgt tggtgccgag catgatccag aagtcctcgc
      181 ccctctggat caggcggagc tccccttcgc caccgggaat cggggcgctg tcgatcagca
      241 cgcgcggaat catgaggcgg gccattagac catatatgga caagcgcaac gaagccgccg
      301 acacaggcgc ctggacgaga aggatgaagc cgtgagcgtc gcattccgcc gcgagagcga
      361 cgaggagcat aaggagccga ggttcgagct gccgatcccg gtcggcccga acctggtgac
      421 cgcgaacggc ctcgccggga tcgaggcccg ggtagttcag ctcgaagcct cggtcgctgc
      481 cgaaaccgaa gaggaacccc tggcgatcgc gaagcgcgag cttcgctatt ggcgcacccg
      541 cctcgcgacc gctcagctgg ctccgccgcc gcccggggac gaagtcgcct tcggctcgcg
      601 ggtcgctttc cggcagaacg gcgcccttcg aagcatcgac atcgtgggcg acgacgaggc
      661 ggacccctcg gcgggccgga tctccttttc ggcgccgctg gcgcgcgccc tgatcggcgc
      721 ctgcgtcggc gaccggctcg atttcgccgg ccggccggag gcgctcgaag tggtcgagat
      781 cggtccgatc ccggactaaa gcgggatcgt ctcggggtga cccgctccgt cattgcgagc
      841 gaagcgaagc aatccagaag cggcttccga ggcgctggat tgccgcgtcg cttcgctcct
      901 cgcaatgacg gctcaaaggc gaaggacgct atttcgataa agcgaaatcg acccttatct
      961 ggacctcgcg ggtgctgatc ccggcctgca cgggaggggc cgaggttgcg acgacggggg
     1021 gcggagcggc ggcctgctcc atcatgaccg cgtcgccatc gtaatggcgg tagggcgagg
     1081 cgattccggc atcggtgatc gtcagcaccc gcgccacctt cagccccgcc gcctgggcat
     1141 aagcctcggc gcgtccgcga gcggcccggt aggccgccgc ataggccgag cgcccggcat
     1201 tttccggatc ggaaacgcgg aggttcggcc ccgagagcac gttggccccg gcttcggtga
     1261 tggcggccag agcttcgccc gcccgcctga tgtcgcggac cttgatctcg atgaggttgt
     1321 tggcctggaa gcggccgcgg ttggggccgt aatcgattcg ggccaaagcg atcgagcggg
     1381 tctggaggtc gtcctccttg accccgagcc gctgcagcgc cgccgcgacc ttgttgatcg
     1441 tggcgctgtt ccgggcgctc gcctcgcccg aggtcgcggc gatcgtgtcg actccgactg
     1501 tgaagcgggc ctcgtccggc ctggtgtcgg cccttcccga ggcggagacc tggaccagca
     1561 cttcgtctcg ggccaccccg cggggatcgg gcgcggcctt cgagcaggcg ccgagggcaa
     1621 gggccaggcc gagggccgaa aacaggtcgc gcatggcgtt actcccagtc gtttggcgcg
     1681 atataacatc acttcaccgc gagaggccag tccgggcccg aaacctcttg aaatatggcc
     1741 gaaatgtgtc tatctagctg ttatgcaaca acttatccgc ttcgcagcgt tcgcgctggc
     1801 cgccctcctt gtcgtcctca tggcttcacc gtcggccgcc caggagcggg ccgaggggca
     1861 gagcaaggcg gattatctcg cctggctgtc gcgcgatgcc ggcgcccggg cgcaggtcct
     1921 ctccttcaaa gccttcctcg aggccggcga cgtcgaggac gtcgtcccga cctggcaact
     1981 ggtgcgcacc gccagcaagt ggcgcgactg cggcggcccc cgcttcgagg tcgctccggt
     2041 cgccgaatgg gcgaacatct ccgccacgct cgacttcatc aaggccgagg tcgagccggt
     2101 gatcggcgac gtccagccgg tttcgggctt ccgcaacgcc gagctcaacc aatgctccgg
     2161 cggcgccaag gcgagcgccc accgccattt ctacgcgctc gacctggtcc cggccgacga
     2221 ggacgtctcc cgcgccgcgc tgatccgcga cgtctgccgg atccacggca atagtggccg
     2281 cgagttcggg atcgggctcg gcttctactc gggcatgcgc ttccacctcg actcgaaggg
     2341 ctatcgccgc tggggcgcca acggcagcgg cgcgaccagc ccctgcgcga cgggcaatta
     2401 cgcgtagagc gagttcgacc aaggtcgaat cgctccaggg tttttcaagc cgcgttttcc
     2461 gagccgctgg ctgttccagc cagctcggaa acgctccagc agcgacgatc gtccgccccg
     2521 caggggacag tcaccagcga ctgtcccctt ttttttgcgc cgggcggcag ggtcgcgccc
     2581 gcgctccggc tgccgcaaca tccggaacgg atgaacttga aggattattg taggattagt
     2641 tctggttttg ttctctcatg atgagcgaat cctccccgag tcggccgcca gatcagagaa
     2701 gagggcggcg tcgagtggaa ggttttctct ttgctccagg agaatgacca tgaaccgcac
     2761 ctacgacgtc gtcgcgatcc aaatgaccat ctggttcaat gtcgagggcg accacgacca
     2821 caatggcctg atctacgtcc tccggcgcaa cctgccgctg gtgaaatatc tgcgcgagct
     2881 cgccaagccg attcccgagc aggccgagct cgaccggctc cgcgacgccg ccaaggagat
     2941 cgcctccgaa ttccaggtgc cgttcccgac cacctccgag gaagcgaaga agccgcacga
     3001 attcgtccgt ccgctggtgc tgcgcgccca tcacggcgac gtcgtgacgg tgaacctgga
     3061 gaacgagatc cagggccggg cgatcggcat gcacctcgtc ggccccggct atgacgtgct
     3121 gaagcatgac ggctccgacg tcggcgccaa cccgcccagc ctcgcgcctc ccggcgggaa
     3181 gtacagctat gtgtggaccg cccagaacca gggcgtgttc ccgttccacg acggcggcga
     3241 ctattcgggc ggccaggacg gcaccaacgt ccacggactg ttcggagcct tcatcgtcga
     3301 gccggtcggg acgatctggc gcgatcccgt ctccggccag gattcgagcg tcgagggcgt
     3361 ccagctcgac ggcctctacc tcgacatcat cccgccggaa gccctgatcg cgaggcccct
     3421 cccggtgccg ccgacgatcg agaactacca gttcccggca ccggtcgaat atacggacta
     3481 cagccgcgag gcgcaccgcg agttcgtcgt cttcttccat gacgagcccg agttcgtgcc
     3541 gccgcacggc acccattatc ccgatccgtg caaccccgac ggcggcggcg gacatggcgg
     3601 acatggcggc gaccacgtcc cgccgatcat gccggtctcc tatcgcgccg agccgatggt
     3661 caacaaggag cagatcctgt tccgtctgct ggcccagggc cacgacttca agggccgccc
     3721 cgtgctcaac gaggagcagc accacagctc ctggatgttc ggcgatcccg cgaccccgat
     3781 cttcaaggcc tatatcggcg acccggtgcg gatccgattc gtccacgcgg cggtcaagga
     3841 gacccacgtc tttcacctcc acctctacga atggcatgcc gcgcccgagg accggaaatc
     3901 gccgcgaatc gacgcgatct cggtgagccc gcagaccggc catacgatcg agcccgtctg
     3961 gggcgcgggc aaccgccacc aggtggcggg cgacgtgatc tggcactgcc acctctaccc
     4021 gcacttccac gaaggcatgt ggggcatgtt ccggaccttc gagacgctcc aggaaggcag
     4081 tgacggcccg ctcctcgcgc agcaggactt cccgtacacg ggccgccgga tcggccgcta
     4141 cccgaacgac acgaggatcg agcggctgct tccgcttccc gggcgggttc cgccgccccg
     4201 gccgactccg aagcatcccg gctacccgct ctacatcccc ggcgagatgc agcagaagtc
     4261 gccgatcccg ccctggccga tgtcgacggc gatgccggtg gaatatgatt atcgccaggt
     4321 tccgacgggc ctcgaacgca acgcgttcaa cccgcagccg gtgccgggcg agatcttcac
     4381 ccgccgtccg ttcgcgctgg ggcagagcca ggagtggccg gaagggcaga ccatgcctgc
     4441 ggacaagccg gcctatcatc tgaacaacgc ccggatggtc cgtcacaatc tgacggtgaa
     4501 catggcccgg atcgactata acggccatgg ctggcacgac aagcacggcc atttctactc
     4561 tctcgccgac gggctcaagg ccaccagcga tcaaacgctg atccgcccgc cgaaggaacc
     4621 gctcttcttc cgcgcccgcc acggccagat cctcaacctg accctgacca accagctccc
     4681 gctcgagatc ccggagacgg agttcgacca cgccttcccg ccgtgcccgg cgcggccgtg
     4741 gcagggcgag tgcacgccgc acgtccacat ggtgaagttc gacccgatct gcgccgacgg
     4801 cgccagcgtc ggctggaact acatctcggg cccggtcgcc ggaaagacga tggcctaccg
     4861 ttggtggctg gaccaggagt tcgggacgat cttcttccac gaccatctgt tcgccaactt
     4921 ccggcagaag cacggcctgt tcggagcgct tatcgtcgag ccggccggcg cccgcttcct
     4981 ccacaacagg gaggaacggg agatcgtcag cggcgcccag gcgcggatcg acgtgcccgg
     5041 ggcgactccg aagtcgttcc gcgaattctg catggggctg caggacttca ttccgatgtg
     5101 ggacaagaac ggcgaggctc tcaacccgcc gccggctccg ggcggtcacg gcgaccaggg
     5161 gatcatggcg atcaactacc gcaacgaccc gatccacgag cggctgaagg acgggagcgg
     5221 cgcgttcatc gacccttcgc tctggttcac ctccgggccg ccctacaacc gcgacccgta
     5281 cacgccgcgg ttcgacaccc tggtgaccga cccgatctgg ttccgggtcg ttcagggatc
     5341 gcacgaggag cagcacagct tccagatcca cgggatgcgg tggcggcggt tccgcgacaa
     5401 cccggcttcg ccgctccgca accagcagag caccggaatc tcggaggcgt tcaccttcaa
     5461 gcaggaggag cgctatcgcg ccggcgacta tatgtacaag ctgtcgggcg cggacgacct
     5521 ctggctgggc tgctggggcg tggttcgggc gttcaattac gacgatgccg aacggccggc
     5581 ggcgctgacc aatcgagtgc ccctgggcac cgacgtgatg tcgagcgccg tccgtacgtt
     5641 ccacatcact gcggaacgga ggcggctcgt ctaccgcgat ccggagctcg tcgatccctt
     5701 cggactgatc taccggctga ccaggatcgt cggcccggac ggcagggata tccccgtgcg
     5761 ccagcaacag acgccgggcc gcgaagagcc gctgatcctt cgctgccgcc gaaacgagat
     5821 ggtgacgatc gtcctgacca acgggatccc ggcggatgcc gacatgcggc ccgaaccatt
     5881 cgccccgagc gttccggtag aggagttcga cgggcaatac cgtccggtgc ggccggtctc
     5941 caggcatgtc tcgatgcacg ccgatctcgt gcgctacgat ccgaccaact cggacggcgc
     6001 caccgttggc cgcaacccgc gccagtcagc ggcgccgggc cagaccgtca cctactattg
     6061 ggtgccgcat ccggcggacc tgccggagtc cggtgagccg atcggcccgg tgctcctcca
     6121 ggacatggcg gacttccgca accaccggca ccacggcctg atcggcgcac tgatcgtgga
     6181 accggcggac gcccggccgc tctacgtccc ggaggggaag aatacggggg acccggtgct
     6241 cgcgtcggac accgtagacg atgcccggcc cggggaagcc tgggacggtc cccgggcgac
     6301 ggtcatcgcc actgtgaacg gccggcagac gaagttcgag gaagcggtgc ttctgcttca
     6361 ggacggactt cgccacttca tccggggcaa cctcacgttg ccggtctcgg acgagccgcc
     6421 gacgccgggc gaggacgggg tcgaccatga ggaccagggc cagaaggggt tcaactatcg
     6481 ctcggagccg atcgagcggt acctcgacaa ccccaatccg gcgactccgg tcttccgggt
     6541 gccggagaat tccagcgtcc ggctccacct ggtcggcgcc ggcgacaagc cccgccagca
     6601 cagcttcacc gttcatggcg tggcctggcc ggagcacaga tacatgccca agatctcgcc
     6661 gagggtggct tcggaatcgg cgatctcgaa cggcacggcg aggaccttcg agttcaacgt
     6721 gggagcgccg gccgactatg cctaccggag cggagtgctc aaatgggccg tcccgcaggg
     6781 cctgtggggc ctgttccggg tcggctaaga ccggcactgc gccgcttgag gcgatcgggg
     6841 ggacagtcac gagtgactgt cccccctttt tttacgactg ttcgagtcga gtcggcgacc
     6901 tccgaaggct ccggccgacc gcgaaaagta ggtccgaaac ggatgatcct gtaggatatc
     6961 gcagagactg ttccgcttat gttcgaggca tgacgaacga atcggcccga cccggcggca
     7021 aggtaatcgc aaatgtcgtg aagctcccgc cggagctgtc gcttttgcag caactccgct
     7081 tgccgcgtcg gaacggtgca ccgggaaact acggtaaata cccttcgatt cggatcgaca
     7141 acgtaacaaa acagatcgtc gccaattcat tgacgatttc cgccaagctg ctgaaaagac
     7201 aacgttgctt gccgttgcgc agtgactcgt ttcctcggga acggcgcttc ggaacggagc
     7261 tcgattgggc gtcgcgcagg ggctgcgggg cctgttccgg gtgggcgcgt agaggaagcc
     7321 gggggacgga cccgtggccg tcccccgtgc ccgcccgggc gaagccaccc agtgcggccg
     7381 aaaggtccgc tgggtggctt ttgccgttgc ggggccgcga ggccctgccg atcaggatat
     7441 ggttcagtga agttcttcgt cccctacgcc gccgacgacg cccatgccga gcagatctgg
     7501 agcgacgccc gggccgacct gttcgacctc ggccttccca ccacccggcg ccggatctgg
     7561 gccctgtcgc tcgacgtccg caatcccggg cacctcctcc acatcggcgg cgaggtcccc
     7621 gacgattacg ggcctgccat gctcatcttc gaggcgagca acctcgacgt cttctatgtg
     7681 tgcacgccct ggaacggggt gctgggcggg gagcctttcg tcctcggcct cggcgagctc
     7741 gggcgggcga tcgatttcga ggaggtcgtc gagggcaatg cctgaggccg cgaggtgtgg
     7801 atccacgcgc tcgttcagga agccgctcag gcagctcccg ctatcgccgc tcctcgacct
     7861 ccttcacctc gaatcccacg atctttccgt cccaccggca ctgcaacgac cgcttccgcc
     7921 cttccccctc gacgctgccc cacacgacga gcttggcggg gctgcgatag acagtgtcga
     7981 tgatccgcac cgtcccatat tgcgcggcgc gcgcgctgca ggcggcccgg gcggcggggc
     8041 cgggatcctc cctgggcgcc ctgtccggcc ggtcggcggc ccggacggcg gcgccgacgg
     8101 cgctcgcggc gaggccggcc gcgcagccgc cgagcggaag ggcgccgagc gcgagtagaa
     8161 gcaggatcga tcggcgcatg tctgttcctt gcctgaagcc cggcatcccg gccggccggt
     8221 ccggattgga cttggagccg cgcccgcgtc aacgcctcga agcggaccgc gctccgtcgg
     8281 gcgggcccgc cggcctcccg tcatcctgaa cttgtttcag gatgcagggt acgagccgcg
     8341 ccgcatcgcc gagcgcttca ccttcacggc cgccagatcc tggtttttcc tgaaggaagg
     8401 cgcgaaggcg cgaaggcggc gatcgccgcg aagccgcccg gcacccgacc gctcccgcct
     8461 ttgcctcccc gcgcccctct gctagggcac ccgcaacccc tcagaaaagc tgaagatgac
     8521 tgatttaaat aaaattagaa atttttccat cattgctcac atcgatcacg gcaagagcac
     8581 gctcgccgac cggctgatcc agtcgacggg cgggctgacc gagcgcgaga tgaccagcca
     8641 ggtgctcgac aatatggaga tcgagcagga gcgcgggatc acgatcaagg cccagacggt
     8701 gaggctcgac tatcgcgcga aggacggcga gcattacgtc ctcaacctca tggacacgcc
     8761 cggccatgtc gacttcgcct acgaagtgtc gcgaagcctg gccgcctgcg agggcgccct
     8821 gctcgtcgtc gacgccgccc agggggtgga ggcgcagacc ctcgccaacg tctaccagtc
     8881 gatcgagcac gaccatgaga tcgtcccggt gatcaacaag atcgacctcc cggccgccga
     8941 cgtcgacaag gtcaggaagg agatagagga tatcgtcgga ctgcccgccg acgacgccgt
     9001 cctcacctcg gccaagaccg ggatcggcat cgccgaggtg ctcgaggcga tcgtcacccg
     9061 aattccgccg ccgaagggag accgggacgc cccgctcaag gcgatgctgg tcgacagctg
     9121 gtacgacccc tatctggggg tcgtcatcct ggtgcgggtc atcgacggct cgatccgcaa
     9181 gggccagaac gtctcgttca tggccgcggg caccacccac ctggtcgacc gcgtcggcgc
     9241 cttccggccc aagatcgagc agctccccga gctcggcccg ggcgagatcg gcttcatcac
     9301 cgcccagatc aaggacatca ccgaaacgcg tgtcggcgac accatcaccg acgcccgccg
     9361 tcccgccgcc gaggctctgc ccggcttcaa gctcgtccag ccggtcgtct tctgcggcct
     9421 gttcccggtc gacgccgcgg atttcgagaa attgcgcgag agcatctcca agctccgcct
     9481 caacgacgcg tcgttcagct tcgagatgga gacgtccgcg gctttgggct tcggcttccg
     9541 ctgcggcttc ctcggcctgc tccacctcga gatcatccag gagcggctga cccgcgaata
     9601 tgatctcgac ctcatcacga ccgcgccgag cgtggtctac ctcgtccacg tcagcaaatc
     9661 gaaggacgcg acttcgacgg gctcagcccg aacggttggg acggacgagg cggggctgaa
     9721 ggtg
//
LOCUS       DASWNU010000348         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_401941, whole genome shotgun sequence.
ACCESSION   DASWNU010000348 DASWNU010000000
VERSION     DASWNU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401941"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VGB57_07005"
     CDS             <1..160
                     /locus_tag="VGB57_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY0131136.1"
                     /translation="AVVVPLYGAPNDEAAVAAVGRRFPGRRVVGIQAPHALTGGGSFH
                     CISQQMPK"
     gene            complement(253..723)
                     /gene="argR"
                     /locus_tag="VGB57_07010"
     CDS             complement(253..723)
                     /gene="argR"
                     /locus_tag="VGB57_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEY0131137.1"
                     /translation="MTDARLRRQKAIADLIRSEPLGSQEEVTARLGAIGYSVTQATVS
                     RDLDQMGAVKVKRGGALSYALPDQAGANDWAAARLQRILAEWVLSVEAAGNLLVIRTP
                     PGSAHLVGLALDQARLDEVAGTICGDDTLFVALRDGIEAGIMADRFRKLSQEGA"
     gene            complement(911..1780)
                     /locus_tag="VGB57_07015"
     CDS             complement(911..1780)
                     /locus_tag="VGB57_07015"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY0131138.1"
                     /translation="MTRLLTVTDLGREGLDEILALSELPDLGLPLAGKGVALVFQKPS
                     ARTRNSMEIATVQLGGHPVYIQKEEVGLGTRESAEDVARTLACYHSVIAARVMDHEDL
                     EAMAAATGKPLLNMLSDRAHPLQALADLLTVRQLLGRLEGARIAYVGDGDNNVCRSLA
                     EACSMLEVELRIASPEGYGLGDAPDGVTQLADPAEAVEGVDIVYTDVWVSMGQDGESE
                     ARAKAFEPYRVDEALMEGCAGARFLHCLPARRGEEVSAAVIDGPRSAVWLQAENRMHT
                     ARGALAWLMGVRP"
     gene            complement(1872..2741)
                     /gene="argB"
                     /locus_tag="VGB57_07020"
     CDS             complement(1872..2741)
                     /gene="argB"
                     /locus_tag="VGB57_07020"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0131139.1"
                     /translation="MRSPTETASILVQALPYIQRFHGRSVVVKLGGAAIDAAIDRALA
                     QDVLLLRSVGVRCVLVHGGGPQVDAMMRRVGKEPEFRDGLRVTDAETLEIVRMVLVGK
                     INRDLVSTINREALEEPVAVGVSGEDAGLLTVTPRESSLGFVGDVSHVRASVLERLLD
                     EGLSPVVSTIGADETGQPYNVNADEAARAIAVAMAAEKIVYLTAAPGLLEDVDDEDSL
                     VPRLTSAELRGRIEHDSVSAGMIPKLRACADAVDAGVGTAHIIDGRVPHALLIELLTD
                     EGIGTMVKREKSW"
     gene            complement(2914..4074)
                     /gene="argJ"
                     /locus_tag="VGB57_07025"
     CDS             complement(2914..4074)
                     /gene="argJ"
                     /locus_tag="VGB57_07025"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY0131140.1"
                     /translation="MSVTAAEGFVAAGCHAGIKRRRFDMALVATDDRAPAAAAAVFTR
                     NKFVAPPVILSRRRLEENGGRAAAIIVNSGNANAGTGAAGLADAEAMGAAAAAALEVA
                     AQDVLVCSTGIIGTPLPMDVILAGTPKLARKLSTGGAHDAARGILTTDHRAKEVVVKG
                     STFTLGGMAKGCGMIAPDMATMLAFLTTDADVSADEMTRILREASDRTFNTLNVDGAT
                     STNDTAILLASARRGRPDLAEFADAVHRACEDLTLQMARDAEGMTKMVALAVTGAASD
                     AEARIAAKRIAENNLVKCSWYGGDPYWGRLLAAAGSAGIAFEAEKATVAYGGTIVSRG
                     GTNAEHDSEAVAAHMKGERIEIEVELGLGTGRAQVIGIDLGPGYIKENSRTS"
BASE COUNT          590 a         1468 c         1419 g          668 t
ORIGIN      
        1 ggccgtcgtg gttccgctct acggcgctcc gaacgacgag gcggcggtgg cggcggtcgg
       61 caggcgcttt ccaggccgga gggtcgttgg aatccaggct ccgcacgccc tgaccggcgg
      121 cggcagcttc cattgcatca gccagcagat gccgaaatga cccgtcgccc cagcgaaggc
      181 tggggccccg gcgggtgaag agcgcgaatg gcttcactcg ccgggatgcc agccttcgct
      241 ggcatgacgg cttcacgctc cctcctgcga aagcttccgg aagcggtcgg ccatgatgcc
      301 cgcttcgatg ccgtccctca gggccacgaa caatgtgtcg tcgccgcaga tcgtgcccgc
      361 gacttcgtcg agcctggcct ggtcgagggc gaggccgacg agatgcgcgg agccgggcgg
      421 ggtcctgatg acgagcagat tgccggccgc ctcgacggaa agcacccatt cggcgaggat
      481 gcgctggagg cgcgccgccg cccagtcgtt ggcgccggcc tgatcgggca gagcgtagct
      541 gagcgctccg ccgcgcttca ccttgaccgc tcccatctgg tcgaggtcgc gggacaccgt
      601 cgcctgggtg accgaatagc cgatcgcccc gagccgggcc gtcacctcct cctggctgcc
      661 gagcggctcg gagcggatca gatcggcgat cgccttctgc cgccggaggc gggcgtcggt
      721 catctcttgt cctccccatc ggagatgggg agggggacca cccgaagggt ggtggtgggg
      781 taattggcga ggccttcggt tttcttttcg gagcctcggg cttcagcgcc ggctacccct
      841 ccaccatgct tcgcatggtc cccttcccca cgagcttcgc tcgcggggag gatgaggttc
      901 gaccattccc tcacggcctc acccccatca gccaggccaa ggcgccccgc gccgtgtgca
      961 tccggttctc cgcctggagc cagacggcgc tgcgcggccc gtcgatcact gcggcgctca
     1021 cttcctcgcc gcgccgcgcc ggaaggcagt ggaggaagcg ggcgccggcg cagccctcca
     1081 tcagagcctc gtcgacacgg tagggctcga acgccttcgc ccgcgcctcc gactcgccgt
     1141 cctggcccat cgacacccag acgtcggtat agacgatgtc cacgccctcg accgcttcgg
     1201 cagggtctgc gagctgggtc acgccgtccg gcgcgtcgcc cagcccatag ccttcggggc
     1261 tcgcgatccg aagctcgacc tccagcatcg agcaggcttc ggcgagcgag cggcagacat
     1321 tgttgtcgcc gtcgccgaca taagcgatgc gcgcgccttc gagccggccg agcagctgcc
     1381 tgaccgtcag caggtcggcc agcgcctgaa gcggatgggc gcggtcggac agcatgttga
     1441 gcaagggctt gccggtcgcc gccgccatgg cctccagatc ctcatggtcc atcacccggg
     1501 ccgcgatcac gctatggtag caggcgagcg tccgggcgac gtcctccgcg ctttcgcggg
     1561 tgccgaggcc gacttcctcc ttctggatgt agaccgggtg gccgccgagc tggacggtgg
     1621 cgatctccat cgagttgcgg gtccgggcgg agggcttctg gaagaccagc gcgacgccct
     1681 tgccggcgag cggcagtccg aggtccggca gctcggacag ggcaaggatc tcgtccagcc
     1741 cctcgcgccc gaggtcggtg acggtgagga ggcgggtcat tcgcccatcc ccccgccgcg
     1801 ctcgaaaaca gggacagtcc ctctttttga agggcgcgga cgaaaaagag ggactgtccc
     1861 tgttttccgg gtcaccagct cttctcccgc ttgaccatgg tcccgatccc ttcgtcggtg
     1921 agaagctcga tcagcaaggc gtgcgggacc cgcccgtcga tgatgtgcgc cgtgccgacg
     1981 cccgcgtcga ccgcgtcggc gcacgcgcgg agcttcggga tcatccccgc gctgacgctg
     2041 tcatgctcga tccgtccgcg cagctcggcc gaggtcagcc ggggcacgag gctgtcctcg
     2101 tcgtccacgt cctccagaag gccgggcgcg gcggtcaggt agacgatctt ctccgccgcc
     2161 atcgccaccg cgatcgcccg ggccgcctcg tcggcgttga cgttgtaggg ctggccggtc
     2221 tcgtcggcgc cgatcgtcga gacgaccggg ctcagcccct cgtcgagcag ccgctcgagc
     2281 accgaagccc ggacatggct gacatctccg acgaagccca gcgacgattc gcgtggagtg
     2341 accgtgagga gccccgcatc ctcgcccgag actccgaccg cgaccggctc ctcgagcgcc
     2401 tcgcggttga tggtcgagac gaggtcgcgg ttgatcttgc cgaccagcac catccgcacg
     2461 atctcgaggg tctcggcgtc ggtcacccgc aggccgtcgc ggaactccgg ctccttgccg
     2521 acccggcgca tcatcgcatc gacctgcggc ccgccgccat ggacgagcac gcagcgcact
     2581 ccgacgctcc tcagcagcag cacgtcctgg gccagcgccc ggtcgatcgc cgcgtcgatc
     2641 gccgccccgc ccagcttcac caccaccgag cggccgtgga agcgctggat gtagggcaaa
     2701 gcctggacga ggatggaagc ggtctcggtg ggggacctca tcagacaaat cctccctgcc
     2761 gcgaagcggt ggggaggggg accacccgaa gggtggtgga ggggtagctg gcgcaggcct
     2821 cggcaattac ccctccaaca tgcttcgcat ggtccccctc cccaccgcct tcggcgatgg
     2881 ggaggataaa gttggcgagg ccagtgcctc tcctcatgac gtcctcgaat tctctttgat
     2941 gtagccgggg ccgaggtcga tcccgatcac ctgcgcccgg ccggtgccga ggccgagctc
     3001 gacctcgatc tcgatccgct cgcctttcat gtgcgcggcg acggcttcgg aatcgtgctc
     3061 ggcgttggtg ccgcccctcg acacgatcgt gcccccatag gcgacggtgg ctttctccgc
     3121 ctcgaaggcg attccggccg atccggccgc cgccagcagc cggccccaat agggatcgcc
     3181 gccataccag ctgcacttga ccagattgtt ctcggcgatg cgcttggccg cgatgcgcgc
     3241 ctcggcgtcg gaggcggcgc cggtgacggc gagcgccacc atcttggtca tcccctcggc
     3301 gtcgcgcgcc atctgcaggg tgaggtcctc gcaggccctg tgaaccgcgt cggcgaattc
     3361 cgccaggtcg ggccggccgc gccgcgccga ggcgagcagg atggcggtgt cgttggtcga
     3421 ggtggcgccg tcgacattga gggtgttgaa ggtacggtcg gaagcttcgc gcaggatcct
     3481 cgtcatctcg tccgcggaga cgtcggcatc ggtggtcagg aaggcgagca tcgtcgccat
     3541 gtcgggcgcg atcatcccgc agcccttggc cattccgccg agggtgaagg tcgagccctt
     3601 cacgaccacc tccttggccc ggtggtcggt ggtcaggatc ccgcgcgccg cgtcgtgcgc
     3661 tcccccggtc gacagcttcc tggcgagctt cggagtcccg gcgaggatga cgtccatcgg
     3721 caaaggggtg ccgatgatcc ccgtcgagca gaccagcacg tcctgtgccg ccacctcgag
     3781 cgccgcggcg gcggccgccc ccatcgcctc ggcgtcggcg aggcccgcgg cgccggtgcc
     3841 ggcattggcg ttgccggagt tgacgatgat cgccgccgcc cggccgccat tctcctcgag
     3901 gcggcggcgc gacaggatca cgggcggagc gacgaacttg ttccgggtga acaccgccgc
     3961 ggccgcggcc ggcgcgcggt cgtcggtggc gaccagggcc atgtcgaagc gccgccgctt
     4021 gattccggca tggcagcccg cggcgacgaa gccctcggcg gcggtgacgc tcatggatag
     4081 actcccgcga tcggcagtcc ggccgtctcg tcgaggccga gcatgaggtt ggcgcattgg
     4141 atcat
//
LOCUS       DASWNU010000349         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_91252, whole genome shotgun sequence.
ACCESSION   DASWNU010000349 DASWNU010000000
VERSION     DASWNU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91252"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..633
                     /locus_tag="VGB57_07030"
     CDS             49..633
                     /locus_tag="VGB57_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY0131141.1"
                     /translation="MKTYLYAFAAVAAIAAAAAPASAEGFRAEVHGGWDHVRSDGPGI
                     NNDDSDSGIVYGIGAGYDFSIAPKVELGVDLSADLSTMEECESSVVLANDRACLDAGR
                     DLAAAVRLGYKVSDRGTLYALAGYTNARFRFDYTTPAGVTTRDGRNLDGFRLGAGYQH
                     DFGKRMYGKVEYRYSNYEAGVTRHQALLGVGVKF"
     gene            complement(863..1000)
                     /locus_tag="VGB57_07035"
     CDS             complement(863..1000)
                     /locus_tag="VGB57_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131142.1"
                     /translation="MTDNKKKGDFSKNGKDDVGGDKKQRSDRNENSRTGSQNSGGGGR
                     D"
     gene            complement(1139..>1901)
                     /locus_tag="VGB57_07040"
     CDS             complement(1139..>1901)
                     /locus_tag="VGB57_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="spore coat biosynthesis protein F"
                     /protein_id="HEY0131143.1"
                     /translation="IAPLPPPRTDILNADQPRILVLAGRRSATLDPLAAKMGVSHKAL
                     VPVAGEAMVGRVLRIAAEPTVARLHSEGRLTAVEAEHHIVDSVVAASRTTGFPLILCT
                     ADNVLMSVEGFRSIADAGSRPGVDGVVMMAEKKDILAAHPDGQRRFYRFRGGAYSNCN
                     LFWLSGPQAFKAIESFRYGGQFAKRKRDAVRALGLTSLFLYVSGLVTLEGMFRHFSRR
                     FGVRIRALVAKDGRLAIDVDNERTHRVAEELLART"
BASE COUNT          295 a          668 c          625 g          313 t
ORIGIN      
        1 gaaagtctcc gcccggccgt tgccggagcg gtcagggagt agaatgaaat gaagacctat
       61 ctgtatgctt tcgccgccgt cgccgccatc gccgcagccg ccgcgcccgc ttccgccgaa
      121 ggtttccgcg ccgaagtgca tggcggctgg gatcacgtcc gttccgacgg ccccggcatc
      181 aacaacgacg acagcgacag cggcatcgtc tacggcatcg gcgccggcta cgatttctcg
      241 atcgccccga aggtcgagct cggagtcgac ctcagcgccg acctcagcac gatggaagag
      301 tgcgagagca gcgtcgtcct cgccaacgac cgcgcctgcc tcgacgccgg ccgcgatctc
      361 gccgcggcgg tccgcctcgg ctacaaggtg tcggaccgcg gcacgctcta cgcgctggcc
      421 ggctacacca acgcccgctt ccgcttcgac tatacgaccc cggcgggcgt gacgacccgc
      481 gacggccgta acctggacgg cttccgcctc ggcgccggct accagcacga cttcggcaag
      541 cgcatgtatg gcaaggtcga gtatcgctac tcgaactacg aggccggagt cacccgccac
      601 caggcgctgc tcggcgtcgg agtgaagttc taagtctccc ccgcccctcg ggagactcgg
      661 ccccggcggt tcatcccccc gccggggcct atttttgcct ccctatcgcg cagcgatggg
      721 gagggggacc gcctgaaagg cggtggaggg ggaatggccc aggaagcaga ggttctcacg
      781 agatcctccc gggtcctcgc ctcttacccc tccaccatgc ttcgcatggt ccccctcccc
      841 atgctgcgca tggggaggca aatcagtccc gaccgccgcc gccgctgttc tggcttccgg
      901 tgcggctgtt ctcgttgcga tcggagcgct gcttcttgtc gccgccgacg tcgtccttgc
      961 cgttcttgct gaaatcgccc ttcttcttgt tgtcggtcat gttgctctcc ttcaggctgg
     1021 agaacggccg ggccgggccg gggtttcgcc ccgtcccgtc atcccggcga aagccgggac
     1081 ctcgggagac ccgccggaac ttctcgtcct gaggtcccgg ctttcgccgg gatgacgatc
     1141 aggtcctcgc cagtaactcc tcggcgaccc ggtgcgtccg ctcgttgtcg acgtcgatgg
     1201 cgagccggcc gtccttcgcg accagcgcgc ggattcggac tccgaagcgg cgcgagaagt
     1261 ggcggaacat gccctcgagc gtcaccagcc cggagacgta aaggaacagc gaggtcagcc
     1321 cgagcgcccg gaccgcgtcg cgcttgcgct tggcgaactg ccccccgtaa cggaagctct
     1381 cgatcgcctt gaaggcctgc gggccgctca gccagaacag gttgcaattg gaataggcgc
     1441 cgccgcgaaa gcggtagaag cggcgctggc cgtcgggatg ggcggcgagg atgtccttct
     1501 tctccgccat catgacgacc ccgtcgaccc cgggcctgga acccgcgtcg gcgatggacc
     1561 ggaagccctc gacgctcatc aggacgttgt ccgcggtgca caggatgagc gggaagcccg
     1621 tggtccggct cgcagccacg acgctgtcga cgatatgatg ctcggcctcg accgcggtca
     1681 gccgcccctc ggaatggagc cgggcgacgg tcggctcggc cgcgatcctc agcactcggc
     1741 cgaccatcgc ctcgccggcg acggggacga gcgccttgtg gctgaccccc atcttcgcgg
     1801 cgagcggatc gagagtcgcc gaacggcgtc cggcgaggac gagaatccgg ggctggtcgg
     1861 cgttcaaaat gtcggtcctc ggcgggggaa ggggggcgat a
//
LOCUS       DASWNU010000350         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_376022, whole genome shotgun sequence.
ACCESSION   DASWNU010000350 DASWNU010000000
VERSION     DASWNU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376022"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /gene="ybeY"
                     /locus_tag="VGB57_07045"
     CDS             complement(<1..295)
                     /gene="ybeY"
                     /locus_tag="VGB57_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY0131144.1"
                     /translation="MILVEADVSEEWDSSTDWAQLARAAVRAAVAHGDVPDLLESGIT
                     AEVSVRFTSDEEVKGLNAAWRGKDKATNVLSFPMVEPEHLQSLARTEGGEALLG"
     gene            complement(411..1415)
                     /locus_tag="VGB57_07050"
     CDS             complement(411..1415)
                     /locus_tag="VGB57_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0131145.1"
                     /translation="MPPKKAPDAVAAAPRARLELEFEQPHLLGPLFGEFDRNLVAIED
                     RLGVYIAARGAKVQIEGEPEDAARARDVLLGLYNRLDEGQDIDAGMVDAIIAMSGQPT
                     LDGIITKDDVAEPPKVMIRTRKKTIVPRSKMQAVYMEALGRDEMIFALGPAGTGKTYL
                     AVAQAVQQLIGGSVDRLILSRPAVEAGERLGFLPGDMKEKVDPYLRPLYDALYDMLPA
                     EQVERRIASGEIEIAPIAFMRGRTLNDAFIILDEAQNTTPAQMKMFLTRFGMRSRMVI
                     CGDPLQVDLPEPGKSGLADAVAKVEGIPKIATIRFSAGDVVRHPLVGRIVEAYEGKAS
                     "
     gene            complement(1505..2240)
                     /locus_tag="VGB57_07055"
                     /pseudo
     CDS             complement(1505..2240)
                     /locus_tag="VGB57_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
BASE COUNT          337 a          759 c          757 g          387 t
ORIGIN      
        1 cgccgagcag agcctccccg ccctcggtgc gggcgagcga ctgaagatgc tccggctcga
       61 ccatcgggaa ggagaggaca ttggtcgcct tgtccttgcc gcgccaggct gcgttcaggc
      121 ccttcacctc ctcgtcggag gtgaaccgga ccgaaacttc ggcggtgatg ccgctttcga
      181 ggaggtccgg cacatcgccg tgcgccacgg cggcacggac cgccgccctg gcgagttgcg
      241 cccagtcggt gctactgtcc cactcttccg atacgtcggc ttcgacgagg atcatcggca
      301 cacccccgtc atcccggcga aagccgggac ctcggggagc cccgccgaga cttcgcggcc
      361 cggggtcccg gctttcgccg ggatgacggc aagaggaaag gccggccccc tcacgacgcc
      421 ttcccttcgt aagcctcgac gatccgcccc accaaaggat ggcgaaccac gtcgccggcg
      481 ctgaagcgga tcgtggcgat cttggggatg ccctcgacct tggcgaccgc gtcggccagg
      541 cccgatttgc cgggctcggg caggtcgacc tggagcggat cgccgcagat caccatccgg
      601 ctgcgcattc cgaagcgggt gaggaacatc ttcatctgcg ccggagtggt gttctgggcc
      661 tcgtcgagga tgatgaaggc gtcgttcagg gtgcgcccgc gcatgaacgc aatgggagcg
      721 atctcgatct ctccggacgc gatccgccgc tcgacctgct cggccggaag catgtcgtag
      781 agcgcgtcgt agagcgggcg cagataggga tcgaccttct ccttcatgtc gccgggaagg
      841 aagcccaggc gctcgcccgc ctcgaccgcc gggcgggaca ggatcagccg gtcgaccgag
      901 cctccgatca gctgctggac ggcctgggcg acggccagat aggtcttgcc ggtgcccgcg
      961 gggccgagcg cgaagatcat ctcgtcgcgg cccagcgcct ccatgtagac ggcctgcatc
     1021 ttggagcgcg ggacgatcgt cttcttgcgg gtgcggatca tcaccttggg cggctcggcc
     1081 acgtcgtcct tggtgatgat gccgtcgagc gtcggctggc ccgacatggc gatgatcgcg
     1141 tcgaccattc cggcgtcgat atcctgcccc tcgtcgagcc ggttgtagag gccgagcagg
     1201 acgtcgcggg cccgcgccgc gtcctcgggc tcgccctcga tctgaacctt ggccccgcgc
     1261 gcggcgatat agaccccgag ccggtcctcg atggcgacga ggttgcggtc gaactcgccg
     1321 aacaggggcc cgagcagatg cggctgctcg aactcgagct cgagccgggc gcgcggcgcc
     1381 gcggcgacgg cgtcgggggc ttttttcgga ggcattatgc cgtcgccccg gcgaaagtgc
     1441 ccgtcatccc ggcgaaagcc gggacctcgg ggagacccgc cgagacttct cgtcccgagg
     1501 tcccggcttt cgccgggatg acgatgcccg caaggctgtt cggcccggcc gaggcgatct
     1561 cgacctcgat caagtcaccg atctccgcct ccgtctcgac atggaccgac tgcagccaag
     1621 gcgttttgcc gatcttctgg ccaggccgtt tgccgggccg ctcgatcagc agcctggtgc
     1681 gagtgcccac gcccctgcgg ttgaactcca gctgctgctc gttgagcaga gcctgaagcc
     1741 gctgcagtct ctcgtccatc actgatcggg gcagttgatc ctccatggcc gccgccggtg
     1801 tgcccggccg cggcgaatat ttgaacgagt aagcctgtgc gtagttcacc gcccgaacga
     1861 tttcgagcgt tgcttcgagc tcggcctcgg tttcgcccgg aaagccgacg atgaaatcgc
     1921 ccgagatcgc gatgtccggc cgggccgacc gaacccgttc gatgagcctg agatagcttt
     1981 cggacgtgtg gcttcggttc atcgccttga gcacccgatc gcttcccgac tgaacgggaa
     2041 ggtggaggaa gggcataagg ccgccgacct ccgaatgcgc ccggatgagg ccttcggtca
     2101 tgtcgttggg atggctggtc gtatagcgga tccgttcgag gcccggcagc cggtcgaggg
     2161 cccggatgag gccgtccagc ccctgtgccc ggcccgcctc gtcctcgccg gtccaggcgt
     2221 tgacgttctg gccgagcagg
//
LOCUS       DASWNU010000351         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_13995, whole genome shotgun sequence.
ACCESSION   DASWNU010000351 DASWNU010000000
VERSION     DASWNU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13995"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGB57_07060"
     CDS             <1..235
                     /locus_tag="VGB57_07060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY0131146.1"
                     /translation="ALGGYSGDQRFFLAWAQGWRGKLRDEVARMILVSDPHSPYRWRV
                     DGTLRNIDEFYSAFDVKPGDRLYLAPGERVRVW"
     gene            411..1997
                     /locus_tag="VGB57_07065"
     CDS             411..1997
                     /locus_tag="VGB57_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0131147.1"
                     /translation="MRLAPLFALLLAACATTPEPPTAAERAERWWGDVSTLAAEDMEG
                     RLTGTAGYQRAADYVVSQLQEIGLEPAGTNGFFQPVAFEEQFVDSAGSSAALVAGGRA
                     TSLSIPGDIIIGRGDGRRPGRIEAPLVFVGYGLHMPEAGHDDFAGIDLKGKIAVVVSG
                     GPAEISGALKAHARRDRARLLSERGAAGLLSLTTAKAVEVPWSRAMAQAGQSGMYFAD
                     PVLRDVKTEFFNASFNTDSSEQLFKGSGRTFAEISALADASKPLPKFDLKPSLRATVA
                     MRHRPVTSSNVVARLPGSDSRLRAEHVVFSAHLDHLGVSTPINGDGLYNGAMDNAAGV
                     AGLLDIARSYKARRLKPKRSMLFVFVTAEEKGLLGSRYFALRPTVPKASMVANMNFDM
                     ALPIFPLKSVTALGADESSLGAMAAAVGQSMGLPLVPDPFPDRNSFVRSDQYSFIREG
                     VPALAFKFGFGPKDPEAEIERQWRSTRYHAPSDDLAQPVAKEDAIRLHDFVGELALRV
                     ANAPERPSWGQQSFFRRFAR"
     gene            1994..>2869
                     /locus_tag="VGB57_07070"
     CDS             1994..>2869
                     /locus_tag="VGB57_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131148.1"
                     /translation="MKALAALLLLLAAPAAAQKAAPLLTAAETEALASEISGASARRT
                     VQALSLHHRMRGSEGYRAAAELIRDRLRDYGLKEVDLISLPADGKIFYGTQRSRPAWN
                     ASFAELWEQRQEGGRWRDSERVASWADQPITLAQDSVGGRAEAELVDVGTGSSAADYA
                     GKTVRGKLVLVSGQPEAAAKLAVTERGAAGIISWAQNQRSAWWGEDESLIRWGHLSTW
                     EDPTFAFMVSPARARAWQARLAKGETVRLRAEVKAGRTPGAYLIPTAIIPGKDRSKEI
                     LFSCHLDHPSPGANDN"
BASE COUNT          430 a         1007 c          996 g          436 t
ORIGIN      
        1 ggccctcggc ggctattccg gcgaccagcg cttcttcctc gcctgggcgc agggctggcg
       61 cggcaagctc agggacgagg tggctcggat gatcctcgtc tccgatccgc actcgcctta
      121 tcgctggagg gtcgacggca ccttgcggaa catcgacgaa ttctattcgg ccttcgacgt
      181 gaagccgggg gacaggcttt atctggcgcc gggggagagg gtgagggtct ggtagctccc
      241 cgtcatcccg gcgaaagccg ggaccccagg acgagaagtc acggcctgtc tccccgaggt
      301 cccggctttc gccgcgatga cgggctaggc tgggaagacg ggctcaccgg ctggacaaga
      361 gcacggcggc gggacaaggg gaggggtcct tgccgccgcc ggagtagccg atgcgtctcg
      421 cccctctctt cgccctcctc ctcgccgcct gcgccacaac tcccgagcct ccgaccgccg
      481 ccgagcgggc cgagcgctgg tggggagacg tctcgaccct ggcggccgag gacatggaag
      541 ggcggctgac cgggaccgca ggctaccagc gtgccgccga ttatgtcgtg tcgcagctcc
      601 aggagatcgg cctcgagccc gccggaacga acggcttctt ccagccggtg gccttcgagg
      661 agcagttcgt cgactcggcg ggatcgagcg ccgcgctggt cgcgggcggg cgagcgactt
      721 cgctctccat ccccggcgac atcatcatcg gccgcggcga cggccggagg cccggacgga
      781 tcgaggcgcc tttggtcttc gtcggctatg gcctccatat gccggaggcc gggcatgacg
      841 atttcgccgg aatcgacctc aagggcaaga tcgcggtcgt cgtcagcggc ggccccgcgg
      901 agatttcggg ggcgctcaag gcgcacgccc ggcgggaccg ggcgaggttg ctctccgagc
      961 ggggcgcggc gggcctgctc tcgctgacca cggccaaggc ggttgaggtg ccctggtcgc
     1021 gcgccatggc ccaggccggc cagtccggga tgtatttcgc ggacccggtc ctgcgggacg
     1081 tgaagacgga gttcttcaac gccagcttca acaccgactc ctcggagcag ctcttcaaag
     1141 gctccggccg caccttcgcg gagatttcag cactggccga cgcctcgaag cccctgccca
     1201 aattcgacct gaagccgtcg ctgcgcgcca ccgtcgcgat gcggcatcgg cccgtcacct
     1261 cgtccaacgt cgtcgcccgg ctgcccggca gcgacagccg cctgagggcc gagcatgtcg
     1321 tcttctccgc ccatctcgac catctcggag tgagcacccc catcaacggc gacgggctct
     1381 acaacggcgc gatggacaat gccgcgggag tcgcgggcct cctcgacatc gcccgaagct
     1441 acaaggcgcg gcggctcaag ccgaagcgct cgatgctgtt cgtcttcgtc accgccgagg
     1501 agaagggcct gctcggctcg cgctatttcg cgctccgccc gaccgttccc aaggcctcga
     1561 tggtggccaa tatgaatttc gacatggcgc ttccgatctt cccgctgaag agcgtgacgg
     1621 ctctgggcgc cgacgaaagc agcctcggcg cgatggcggc ggcggtcggc cagtcgatgg
     1681 ggctgccgct ggtgccggac cccttcccgg accggaacag cttcgtccgc tcggaccagt
     1741 acagcttcat ccgcgagggc gttccggcgc tcgccttcaa gttcggcttc ggccccaagg
     1801 atccggaagc ggagatcgaa cggcaatggc gctcgacccg ctaccacgca ccatcggacg
     1861 acctcgccca gccggtcgcg aaggaggatg cgatcaggct gcacgatttc gtcggcgagc
     1921 ttgcacttag ggtcgccaac gctccggagc ggccttcgtg gggccagcaa agcttcttcc
     1981 ggcggttcgc ccggtgaagg ctctcgcggc gctcctcctg cttctcgcag ctccggcggc
     2041 ggctcagaag gccgcacccc tgctgaccgc cgccgagacg gaggcgctgg cgtcggagat
     2101 ttcgggcgcg tcggccaggc ggacggtgca agcgctgtcc ctccaccacc ggatgcgggg
     2161 ctcggaaggc tatcgcgccg cggccgagct gatccgcgac cggctccgcg actacggcct
     2221 caaggaggtg gacctcatct ccctgccggc ggacggcaag atcttctacg gcacgcagcg
     2281 ctcgcgcccg gcctggaacg ccagcttcgc cgaactgtgg gagcagcgcc aggagggcgg
     2341 gcgctggcgc gactccgaac gcgtcgcgtc ctgggccgac cagccgatca cgctcgccca
     2401 ggacagcgtc ggcggccgcg cggaggcgga gctggtcgac gtcgggacgg gcagctccgc
     2461 ggccgattat gccggcaaga cggtgcgtgg gaagctcgtg ctggtctccg gccagccgga
     2521 ggcggcggcg aagctcgcgg tgacggagcg gggcgcggcc ggtataatct cctgggccca
     2581 gaaccagcgc tcggcctggt ggggcgagga cgagagcctg atccgctggg gccatctcag
     2641 cacctgggag gacccgacct tcgccttcat ggtctcgccg gcccgggccc gcgcctggca
     2701 ggcacgactc gcgaagggcg agacggtgcg gctccgggcg gaggtgaagg cgggccgcac
     2761 gcccggcgcc tatctcatcc ccaccgcgat catccccggc aaggatcgtt cgaaggagat
     2821 cctcttctcc tgccacctcg accatccctc gccgggcgcc aacgacaat
//
LOCUS       DASWNU010000352         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_402027, whole genome shotgun sequence.
ACCESSION   DASWNU010000352 DASWNU010000000
VERSION     DASWNU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402027"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VGB57_07075"
     CDS             complement(<1..732)
                     /locus_tag="VGB57_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0131149.1"
                     /translation="MADQSAARLVAEEGEARRIFDLQHAASRQGASAGLELRRDRLER
                     LRAMIAGNEAALAEAISTDFGVRSRTETELLEIVPTLNAIRHARKHVAAWMRPERRRV
                     DPLFQPASARVRHEPLGVIGIISPWNYPLQLALSPLVDALAAGNRAMLKPSELTPAFS
                     ELLRRLVAERFDESEVAVITGGVEVGQAFASLPFDHLLFTGSTAVGRKVYQAAAANLV
                     PVTLELGGKSPTIICPGYDVAKAARS"
     gene            849..>1651
                     /locus_tag="VGB57_07080"
     CDS             849..>1651
                     /locus_tag="VGB57_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0131150.1"
                     /translation="MTIRIGIIGYGKIARDQHVPSIAADPRFELAAVVSPRAPVDEGV
                     PVFPSHEAMLAAMAGDLDAVAICTPPTVRVAVARAAFAAGLDVLLEKPPAATLGEIEE
                     IGRLGRESGQTLFTAWHSQHAAAVAPARAALAGKDVRALQIHWHEDVRKWHPGQEWIW
                     APGGFGIFDPGINALSIATRILPSRLFVREAVLSFPENKQTPIAARIAFEGEAGRFEA
                     DLDWRYTEGERWTIRVETGDGIAIELLDGGARMTVDGEPRPAASPGEYPS"
BASE COUNT          254 a          570 c          581 g          246 t
ORIGIN      
        1 gctccgggcc gccttggcga cgtcgtaacc ggggcagatt atggtcggcg acttgcctcc
       61 gagctccagg gtgacgggca cgagattggc ggctgcggcc tggtagacct tgcggccgac
      121 ggcggtcgag ccggtgaaca gcagatggtc gaagggcagc gaggcgaagg cctgcccgac
      181 ctcgaccccg ccggtgatca ccgccacctc cgactcgtcg aagcgttccg cgaccaggcg
      241 ccggagcaac tcggaaaatg cgggcgtcag ctcggagggc ttcagcatcg cccggttgcc
      301 ggcggcgagc gcgtcgacca gcggcgagag ggcgagctgg agcggatagt tccagggcga
      361 gatgatcccg atcacgccca gcggctcgtg ccgcacccgg gcgctggccg gctggaacag
      421 cggatcgacc cgccgccgct ccgggcgcat ccacgccgcc acgtgcttgc gggcatggcg
      481 gatcgcgttc agggtcggga cgatctcgag cagctcggtc tcggtgcgcg aacgcacccc
      541 gaaatccgtc gagatcgcct cggccaaagc ggcctcgttg ccggcgatca tggcccgcag
      601 ccgctcgagc cggtcccgcc gaagctccag gccggcgctg gcgccctgcc tggacgcggc
      661 atgctgaagg tcgaagatcc ggcgcgcctc gccttcttcc gcgacgagcc tggcggcgga
      721 ttgatcggcc atgctgattc ctcccgaaga gtcttcatag cctatccgcc ccggcctggc
      781 accttttggg ctttgctccg tccagccatc cgtccatcag gcgcgctgac gccagccagg
      841 aggttccagt gacgatccga atcggcatta tcggctacgg caagatcgcc agggaccagc
      901 atgtgccctc gatcgccgcc gatccgaggt tcgagctcgc cgcggtggtg agtccgcgcg
      961 cgccggtcga cgagggcgtc ccggtcttcc cgagccacga agcgatgctt gcggccatgg
     1021 ccggcgatct cgacgcggtc gcgatctgca cgccccccac ggtccgcgtc gccgtcgccc
     1081 gggccgcctt cgccgccggc ctcgacgtcc ttctcgaaaa gccgccggcc gcgaccctgg
     1141 gagagataga ggagataggg aggctggggc gggagtcggg acagacgctc ttcaccgcat
     1201 ggcactcgca gcatgccgcg gccgtggcgc ccgcccgggc ggcgctcgcc gggaaagatg
     1261 tgcgggccct tcagatccac tggcacgagg acgtgcgcaa atggcatccc gggcaggaat
     1321 ggatctgggc cccgggcggc ttcggaatct tcgatccggg cataaatgcc ctgtcgatcg
     1381 cgactcggat cctgccgtcg cggctgttcg tccgggaagc ggttctctcc ttccccgaga
     1441 acaagcagac tccgatcgcc gcccgcatcg ccttcgaagg cgaggcggga cgattcgaag
     1501 ccgacctgga ctggcgctat accgaaggcg agcgctggac gatccgggtc gagacggggg
     1561 acggcattgc tatcgagctt ctcgacggcg gggcccggat gacggtggac ggcgagccgc
     1621 ggccggcggc gagcccgggc gaatatccct c
//
LOCUS       DASWNU010000353         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_298458, whole genome shotgun sequence.
ACCESSION   DASWNU010000353 DASWNU010000000
VERSION     DASWNU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298458"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGB57_07085"
     CDS             <1..349
                     /locus_tag="VGB57_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131151.1"
                     /translation="DGQRTYLDVAYFGGQSVSLAEQMLALPPGRHSLRLIARSPNGIS
                     SGRLFWRLNCLPGGSQIGLLDMGRAGPDNRRFSADFTVPSGCPGQSLSLIAEPGDVAS
                     PVNLEIERMEIGR"
     gene            346..1722
                     /locus_tag="VGB57_07090"
     CDS             346..1722
                     /locus_tag="VGB57_07090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0131152.1"
                     /translation="MRGGFWTLAAAVYLAACLLLGGASAAGVAANAFLQLVGLGLIVA
                     LLWRRDFSLPDGARGPLWIGGALLLVGLISLIPLPAGLGSSPLRAEVVEAFRLIGMEP
                     PALSASLAPAWTLNSLLHLLPAAAMFLLVVRLPNEDRRILPTVLLAVAGASVVLGAFQ
                     LMGGPDSPLRFYEVTNRTSPVGFFSNANHEATLLLCALPLTAVIAGRMATRRSRSKRS
                     GGAIVSVSIAVFILAGLAISGSRAGYGLALPAAMASFLIYRRTVAGRVAAGWWGALAV
                     LALVFAYAALQGPLGKETLQADMAEAPSSRRVLASTTTEIIKDSFPLGTGLGTFSTVY
                     RRYEDPSKVTRQFANHAHNDYLEAVLELGAAGLLLIVAFLLWWGRRLFQAWTRDFEGA
                     ALARAGSVMVGIVLAHSIVDYPIRTAAIVAAFALGCALMIPPMPRRSPAKPAEDEASE
                     PLRHIEAV"
     gene            1754..2314
                     /gene="rfbC"
                     /locus_tag="VGB57_07095"
     CDS             1754..2314
                     /gene="rfbC"
                     /locus_tag="VGB57_07095"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY0131153.1"
                     /translation="MPQDVLIEQTPLAQLLVLTPRFFRDERGFFTESYNKRAFAEAVG
                     ADVEFVQDNQSRSAQGVLRGLHYQLPPRAQGKLVRVVEGEIFDVAVDLRSSSATSGRW
                     FGAILSAEKGNQMWIPPGFAHGFLTLSETADVLYKTSDYYAPDRERTIAWDDPGIAID
                     WPLSGRPLLSAKDADAPTLAQAETFA"
     gene            2311..>2812
                     /locus_tag="VGB57_07100"
     CDS             2311..>2812
                     /locus_tag="VGB57_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0131154.1"
                     /translation="MSADPLYVTRPFLPPLEEFLPLLAEIWNSRILTNQGPFHQRFER
                     ELCELFGVDNVSLVTNGMLALSAVIEAAGVGGEVITTPYSFVATTHAVRMGSLEPVFV
                     DIRRSDLNIDPDLIEAAITPRTSAIVAVHCYGNPCDVESIEAIAARHGLKVIYDAAHA
                     FGVRRNG"
BASE COUNT          397 a         1003 c          924 g          488 t
ORIGIN      
        1 ggacggccag cgcacctatc tcgacgtcgc ctatttcggc ggtcagagcg tgagcctcgc
       61 cgagcagatg ctggccctcc cgccggggcg ccacagcctg cgcctcatcg cccgaagccc
      121 gaacgggata agttcgggcc ggctcttctg gcggctgaac tgcctgccgg gcggaagcca
      181 gatcggcctg ctcgacatgg gccgcgccgg ccccgacaac cgccggttca gcgccgactt
      241 cacggtcccg tcgggctgcc cgggccagtc gctgagcctg atcgccgaac cgggcgacgt
      301 cgcgtctccc gtcaacctcg agatcgagcg catggagatc ggccggtgag gggtggcttc
      361 tggaccttgg cggcggccgt ttatcttgcc gcctgcctgc tgctcggcgg cgccagcgcg
      421 gcgggcgtcg cggccaacgc cttcctccag ctggtcggcc tcggcctgat cgtcgccctc
      481 ctgtggcggc gggatttctc actgcccgac ggcgcgagag gacccttgtg gatcggcggg
      541 gcattgctgc tggtcggcct gatcagcctc atcccgcttc ccgcaggcct cggatcgagc
      601 ccgctgcgcg ccgaggtggt cgaggcattc cggctgatcg ggatggagcc gcccgcgctc
      661 tccgcctccc tggcgccggc ctggaccttg aactcgctgc tccatctgct tccggcggcg
      721 gcgatgttcc tgctcgtggt tcggcttccc aacgaagacc ggcggatcct gccgacggtg
      781 ctgctggcgg tggccggcgc ctcggtggtg ctgggcgcct tccagctcat gggcgggccg
      841 gattccccgc ttcggttcta cgaggtcacc aaccggacct cgccggtcgg tttcttctcc
      901 aacgccaatc acgaggcgac gcttctgctt tgcgccctgc cgctgacggc ggtcatcgcc
      961 gggcggatgg cgacgcgccg atcccgctcc aagcgcagcg gcggagcgat cgtcagcgtc
     1021 tcgatcgcgg tcttcatcct cgccgggctc gccatcagcg ggtcgagagc cggctacggc
     1081 ctcgctttgc cggcggcgat ggccagcttc ctcatctacc gccgcaccgt cgccggacgg
     1141 gtggcggcgg gctggtgggg ggccctcgcc gtgctggccc tggtcttcgc ctatgccgcg
     1201 cttcaggggc cgctgggcaa ggagaccctc caggccgaca tggccgaagc gccgtccagc
     1261 cggcgagtcc tggcctcgac gacgacggaa atcatcaagg acagcttccc gctcggcacc
     1321 ggcctcggca ccttctcgac cgtctatcga cgctacgagg acccgagcaa ggttacccgg
     1381 cagttcgcca atcatgccca taacgactat ctggaagcgg tgctcgagct cggagcggcg
     1441 ggcctgctgc tgatcgtcgc attcctgctc tggtgggggc ggcggctctt ccaggcctgg
     1501 acccgggact tcgagggcgc cgccctggcc cgggccggct cggtgatggt cggaatcgtg
     1561 ctcgcccatt cgatcgtcga ctatccgatc cgaaccgcgg cgatcgtcgc ggccttcgct
     1621 ttgggctgcg cattgatgat tccgccgatg ccgcgacgaa gcccggccaa gcccgcggaa
     1681 gacgaagcgt cggagccgct gcgccatatc gaagccgtgt gactcctcca cacagccgcc
     1741 agacaccgaa ggaatgcccc aggacgtgct gatcgagcag acccctctcg cccaattgct
     1801 cgtgctgacg ccgcgtttct ttcgggatga gcgcggcttc ttcaccgaaa gctacaacaa
     1861 gcgggccttc gccgaggccg tcggcgcgga cgtcgagttc gtccaggaca accagagccg
     1921 ttcggcgcag ggcgtattgc gcggcctcca ctatcagctg ccgccgcgcg cgcagggcaa
     1981 attggtgagg gtggtcgagg gcgagatatt cgatgtcgcg gtggacctgc ggagcagctc
     2041 cgccacttcc ggccgctggt tcggcgccat cctctcggcc gagaagggca atcagatgtg
     2101 gataccgccg ggcttcgccc acggcttcct gacgctcagc gagacggcgg acgtgctcta
     2161 caagacctcg gattattacg cccccgatcg cgagcggacg atcgcctggg acgatcccgg
     2221 catcgccatc gactggccgc tgagcggacg gcccctgctc tcggccaagg acgccgacgc
     2281 gccgacgctg gcccaagcgg agaccttcgc atgagcgccg accctctcta cgtgacccgg
     2341 ccctttctgc cgcctctcga ggaattcctg cccctgctcg ccgagatatg gaactcgcgg
     2401 atcctgacca accaggggcc cttccaccag cgtttcgagc gcgagctgtg cgagctgttc
     2461 ggagtcgaca acgtctctct cgtcaccaac ggcatgctcg ctctgagcgc ggtcatagag
     2521 gccgccgggg tcggcggcga ggtgatcacg accccttact cgttcgtggc cacgacccat
     2581 gcggtgcgga tgggcagcct cgaaccggtg ttcgtggaca tccgccggtc ggacctcaac
     2641 atcgatcccg atttgatcga agcggcgatc acgccccgga cctcggcgat cgtcgccgtc
     2701 cattgctacg gcaatccctg cgacgtcgag tcgatcgagg cgatcgccgc ccggcacggc
     2761 ctcaaggtga tctacgacgc ggcccacgct ttcggggttc gccgcaacgg cc
//
LOCUS       DASWNU010000354         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_247257, whole genome shotgun sequence.
ACCESSION   DASWNU010000354 DASWNU010000000
VERSION     DASWNU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247257"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGB57_07105"
     CDS             <1..203
                     /locus_tag="VGB57_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0131155.1"
                     /translation="HAAARSFLHHQVRSMVGCLELVGRGKWAASDLEAALEAKDRAAL
                     GFNAPPHGLYFVEAIYPSPPLP"
     gene            complement(258..2189)
                     /locus_tag="VGB57_07110"
     CDS             complement(258..2189)
                     /locus_tag="VGB57_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HEY0131156.1"
                     /translation="MCGIAGIFHPDVPKPVDPARVRAMADTLAHRGPDGSGVWTGPGV
                     GFGHRRLSIIDLEGGVQPMVSQGGRLALTYNGEIYNFREVRAELEAKGHAFRTDSDTE
                     VILAAWAEWGPDCLTRFNGMFAFALYDAEDDCLFLARDRLGVKPLYLATLPDGALLFA
                     SELKGLLAHPLLRREISPQAVEDYLGLGYVPDDSCIVEGVSKLPAGHFLLIRRGRRVP
                     APARWWDVDLSSPSRASAASLEEELLHRLGEAVRSRMVADVPLGAFLSGGVDSSAVVA
                     LMAEASRSAVETCSIGFTEADHDETAYAQAVSDRFATNHRTRIVAAGDFSLIDTLADT
                     FDEPFADASALSTYRVSELARERVKVALSGDGADEAFAGYRRYRLFSAEEKVRGLLPQ
                     GARSLFGTLGSVYPKLDWAPQVFRAKTTFQALGRSSEEAYALAVGVTPPLARTGLYTP
                     ILRQGLQGHRAETRYVEAMRAAPANDPLSRAQYADLKNWLPGDILTKVDRTSMAVSLE
                     AREPLLDHRLVELAARLPANLRLRGGTGKYLMKRALRRHLPDEILHRRKQGFVTPISA
                     WFRGPLAAEAGAIARSPALAELGWFDPAAIARLAETHRSGRADSGRLLWQLLMLEKSL
                     QRVFGLGGQIPSPLRERAG"
     gene            complement(2194..3732)
                     /gene="xrtA"
                     /locus_tag="VGB57_07115"
     CDS             complement(2194..3732)
                     /gene="xrtA"
                     /locus_tag="VGB57_07115"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEY0131157.1"
                     /translation="MTAVLPLSEAKPLVAQGWRTHLYALAAVSASILLIFARDAADLA
                     AVWWNSSTFNHCLLIPPIVAWLVWQRLPELRQLTPSAWAPGLLLLSAGAAAWLLGDAG
                     GLALARHAGLVLMLQGAMIACLGKSVSRGLAFPLFYLLFLIPIGEEIVPLMQTLTARM
                     SMALLALSGVPAYIEGVFISTPTGYFEVAEACAGVKVLVAMIAYGALVANVCFRSPVR
                     RTAFMAAAIAIPVLANGVRAWGTIYIAHVTGNIEFAAGFDHIFYGWIFFAIVIVLIMA
                     AGWPFFDRKVADPWFDPAALQARPGTTRRLVQIAAAAVALAALPPIWSAAIASAGTRA
                     APADIALPEVAGWTRVHSDSGRPWEPRFAGADVLRIGRYRNAVGQEVDLAIAVFARQE
                     EGRELVGFGQGAAPPDGGWAWTSDAAPPPSGRAERIAANGALREVVSFYRVGNVLTGS
                     PAAVKLETIKVRLLGGPQRAVALLVSAPAPADEVSPRPAIDAFLAALGPIAPLADRAA
                     GVQD"
     gene            complement(3729..>3971)
                     /locus_tag="VGB57_07120"
     CDS             complement(3729..>3971)
                     /locus_tag="VGB57_07120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0131158.1"
                     /translation="PVVASSCAFEGIEASPGRDLLVADTARDQANAINTLLSDPVRAA
                     AIGAAARRRVEEAYRWDVRLAPLAGLLGLPERQAAA"
BASE COUNT          641 a         1192 c         1611 g          527 t
ORIGIN      
        1 tgcatgcggc ggcgcggtca ttcctccatc accaggtgcg gtcgatggtc gggtgcctcg
       61 agctggtcgg gcgaggaaag tgggcggctt cggacctgga ggccgcgctg gaggcgaagg
      121 accgggcggc gctcggcttc aacgcgccgc cgcacggcct ctatttcgta gaagcgatct
      181 atccttcgcc ccctctccct tgagggagag gatgagattc tcgggcggcc gggatttcct
      241 tcaggcgccg agcaccctca ccctgccctc tccctcaagg gagagggtat ttgcccgccg
      301 agcccgaaga cgcgctgaag gctcttctcc agcatcagca gctgccagag gaggcggccg
      361 ctgtcggcgc ggccggagcg gtgggtttcg gctaggcggg cgattgcggc ggggtcgaac
      421 cagccgagct cggcgagcgc cgggctgcgg gcgattgcgc cggcttcggc tgcgaggggg
      481 ccgcggaacc aggccgagat gggcgtgacg aagccctgct tgcggcggtg gaggatctcg
      541 tccgggaggt ggcgacggag cgcccgcttc attaaatact tgccggtgcc gccgcggagg
      601 cgaaggttgg caggaaggcg cgccgcaagc tcgacgaggc ggtggtccaa gaggggctca
      661 cgggcttcga ggctgacggc cattgaggtg cggtcgactt tggtgaggat gtcgccgggc
      721 agccagttct tgaggtcggc atattgggca cgggagagcg gatcgttggc gggcgccgcg
      781 cgcatcgcct cgacgtagcg cgtttcggcg cggtgaccct ggagaccttg cctgaggatc
      841 ggcgtgtaaa gccctgttct tgcgaggggc ggcgtgactc cgacggccag cgcataggct
      901 tcctcactcg agcggccaag ggcttggaac gtcgtctttg cgcggaacac ctgcggggcc
      961 cagtcgagct tcgggtagac cgaaccgagc gtgccgaaga ggcttcgggc gccctggggg
     1021 agaagccccc tcaccttctc ctcggccgag aagaggcggt agcggcggta gccggcaaag
     1081 gcctcgtccg ccccgtcgcc ggagagcgcg accttgactc tctcccgggc gagctcggag
     1141 acccggtagg tggagagggc ggaggcgtcg gcgaagggct cgtcgaaggt atcggcgagc
     1201 gtgtcgatca gggagaagtc gcctgcggca acgatgcggg tgcggtgatt ggtggcgaag
     1261 cggtcggaga cggcttgggc gtaggcggtc tcgtcatggt ccgcttcggt gaagccgatc
     1321 gagcaggtct ccaccgccga gcgactggct tcggccatga gggcgacgac agccgaactg
     1381 tcgacgcctc cggagaggaa ggcgccgagc ggcacatcgg cgaccatgcg ggagcggacc
     1441 gcctcgccga ggcggtggag gagctcttcc tccaggctgg cggcggaggc gcgggagggg
     1501 ctggacaaat ccacgtccca ccagcgtgcc ggcgccggca cgcggcggcc gcgccggatc
     1561 aggaggaagt ggcccgcggg gagcttggag acgccttcga cgatgcagga atcgtcgggg
     1621 acgtagccga ggccgagata atcctcgacc gcctgggggc tgatctcgcg ccggagcaat
     1681 gggtgggcga ggaggccctt gagctccgag gcgaagagga gcgccccatc ggggagcgtg
     1741 gcgaggtaga gcggcttcac gccaaggcgg tcgcgggcga ggaagaggca gtcgtcctcc
     1801 gcgtcataga gggcgaaggc gaacatgccg ttgaagcgcg tgaggcagtc gggcccccac
     1861 tctgcccagg cggcgaggat cacctcggtg tcgctgtcgg tgcggaaggc gtggcccttc
     1921 gcctcgagct cggcgcggac ctcgcggaaa ttgtagatct cgccattgta ggtgagggcg
     1981 aggcggccgc cttgggacac catcggctgg acgccgcctt cgaggtcgat gatcgagagg
     2041 cggcggtggc cgaagccgac gccggggccg gtccagacac cgctgccgtc cgggccgcga
     2101 tgggcgagcg tgtccgccat cgctcgcacc cgcgccggat cgacgggctt ggggacgtcg
     2161 gggtggaaga taccggcgat gccacacata gagtcagtcc tgaacgcccg cggcgcggtc
     2221 ggcaagcggc gcgatcgggc cgagggcggc gaggaaggcg tcgatcgccg ggcgcgggct
     2281 gacctcgtcg gcgggggccg gtgcggagac gagcaatgcg acggcgcgct gcgggccgcc
     2341 gaggaggcgc accttgatcg tctcgagctt cacggcggcg gggctgccgg tgaggacgtt
     2401 gccgacgcgg tagaagctga ccacttcccg cagagcgccg ttggcggcaa tgcgctcggc
     2461 gcggccggag gggggcggcg cggcgtcgga ggtccaggcc cagccgccgt cgggcggagc
     2521 ggcgccctgg ccgaagccga cgagctccct cccctcctcc tgccgcgcga agacggcgat
     2581 ggcgaggtcg acttcctggc cgacggcgtt gcggtagcgg ccgatgcgaa ggacgtcagc
     2641 gccggcgaaa cgcggctccc agggacggcc gctgtcggag tggacgcggg tccagcccgc
     2701 gacttcgggg agggcgatgt cggcgggggc ggcgcgggtg ccggcggagg cgattgcggc
     2761 ggaccagatc ggcgggaggg cggccagcgc cacggcggcg gcggcgatct ggacgaggcg
     2821 ccgcgtggtg ccggggcgcg cctgaagggc ggccggatcg aaccagggat cggcgacctt
     2881 gcggtcgaag aagggccagc ccgcggccat gatcaggacg atgacgatcg cgaagaagat
     2941 ccagccgtag aagatgtgat cgaagccggc ggcgaactcg atattgccgg tgacatgggc
     3001 gatgtagatc gtcccccagg cacgcacgcc gttggcgagc accgggatgg cgatcgccgc
     3061 cgccatgaag gcggtgcggc ggacggggga gcggaagcag acgttggcga ccagcgcccc
     3121 gtaagcgatc atcgcgacga ggaccttcac gcccgcgcag gcctcggcca cttcgaaata
     3181 gccggtgggc gtcgagatga agacgccctc aatgtaggcg gggacgccgg agagcgcgag
     3241 gagcgccatc gacatcctgg cggtcagcgt ctgcatcagc ggcacgatct cctccccgat
     3301 cgggatcagg aagaggaggt agaagagcgg gaaagcgagg ccgcgggaga ccgacttgcc
     3361 gaggcaggcg atcatcgccc cctggagcat caggacgagg ccggcatggc gcgcgagggc
     3421 gaggccgccg gcatctccga gcagccaagc ggctgcgccc gctgaaagga ggaggaggcc
     3481 cggagcccag gcggaggggg tgagctggcg aagctcgggc aggcgctgcc agacgagcca
     3541 ggcgacgatc ggggggatga ggaggcagtg gttgaaggtg gagctgttcc accagacggc
     3601 cgcgaggtcg gcggcgtctc gcgcgaagat caggagaatt gaggccgaga cggcggcgag
     3661 ggcgtagagg tgagtgcgcc agccttgggc aaccagcggc tttgcctcgg aaagggggag
     3721 aacggccgtc atgccgcggc ctgtcgctcg ggaaggccga ggaggccggc gagcggggcg
     3781 aggcggacgt cccagcgata ggcttcctcc acccggcgcc gggcggcggc gccgatggcg
     3841 gcggctcgaa cggggtcgga gaggagggtg ttgatggcgt tcgcctgatc gcgcgcagtg
     3901 tcggcgacga ggaggtcgcg gccgggggag gcttcaattc cttcgaaggc gcaagagctt
     3961 gccaccaccg g
//
LOCUS       DASWNU010000355         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_481710, whole genome shotgun sequence.
ACCESSION   DASWNU010000355 DASWNU010000000
VERSION     DASWNU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481710"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..978)
                     /locus_tag="VGB57_07125"
     CDS             complement(229..978)
                     /locus_tag="VGB57_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ImuA"
                     /protein_id="HEY0131159.1"
                     /translation="MPRSPARAERLAALRAEVRALESAGSERRLCLPFGVDAIDSRLA
                     GGGLPVASLSEAAGERPGPADQAAATLFLASLAARRPGIVLWALGSRDLFAPGLAGAG
                     LPPDRLLYAECARDEDVLAVMEEGLRHGGLAAVVGEVGRAAMPATRRLQLAAEEGGTL
                     ALMLRRWRGNGEDPLAPPSAAVTRWRLACAPSSPLPVAGIGRPRWRLSLARQRGGAPF
                     DLIMESPDAEGRLALPSEPQHRPGESERRAA"
     gene            1227..>2697
                     /locus_tag="VGB57_07130"
     CDS             1227..>2697
                     /locus_tag="VGB57_07130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0131160.1"
                     /translation="MLRRCLLALLLLVAAPVQAAGDSSWFYKNTDIPPDPAWTFGTLP
                     NGLRYAVRRNAVPEGQVSVRLRIDAGSLHEEEKERGWAHFVEHMAVRGTKNFGDGEVR
                     ETWQRLGASFGSDTNASTEPTQTVYMLDLPRADRASLDTSLRLLADMADTALFDPKIV
                     DTERGVVLSEYGRRTELAVKLREMMMPLFYGGLKYAQRDTIGTEATLKGADAAGLKAF
                     YERWYRPDRATLVMVGDADPRLMEELIAARFGGWKPSGPAPSEPDYGAPADVPQRTAA
                     IAYPGSPHFATLSWIRPYRRVPPSKAREQRNLARTLASRILNRRLEAKARGEAAFLNA
                     QVQAENSVNVADTTQMSVMAKEGRWQQALTEAYAILADALKAPPSRTEIDREMENIRT
                     AGRAAVQGDPTQRSQIRAGRLISAIDGNSVISTAQIQLALFEELAPSMTPEAVQAALK
                     DMFQGSGPRLAMLSPAPVAGLAQALAAAEAAAPAARQADR"
BASE COUNT          425 a          952 c          960 g          360 t
ORIGIN      
        1 ccagggcaag gccgaaggcc agtgcgaacc gtaaagcccc tctcccgctt ttcgcgggag
       61 aatggggcgc ctggctgcgt cccccagacg cagccttggc ggcgccccat gggcccaagc
      121 gaagcttggg aggtgagggt ctgtacaccg gactcggcaa gaccctcacc tcccaagcct
      181 tcggctcggg cccctcctct cccgcggaaa gcgggagagg aattatcgct acgccgccct
      241 tcgctcgctt tcacccggtc ggtgctgagg ttcggaaggt agagcgaggc gaccctcggc
      301 atcggggctc tccatgatca ggtcgaacgg cgctccgccg cgctggcggg cgagtgagag
      361 gcgccagcgg gggcggccga tccccgccac cggcagcggc gaggacgggg cgcaggcgag
      421 gcgccagcgg gtgacggcgg cggacggagg ggcgagcggg tcctcgccat tgccgcgcca
      481 gcgccggagc atgagggcga gcgttccgcc ttcctcggcg gcgagctgga ggcggcgggt
      541 ggcgggcatg gccgcccgcc ccacttcgcc gaccaccgcg gcgaggccgc cgtggcgaag
      601 cccttcctcc atcaccgcca gcacatcctc gtcgcgcgcg cattcggcgt agagcagccg
      661 gtccgggggg aggccggcac cggccaggcc gggcgcgaac aggtcgcggc tgcccagcgc
      721 ccacaggacg atcccggggc ggcgggcggc cagagaggcg aggaagagcg tcgcggcggc
      781 ctgatcggcc gggccgggcc gctcgcccgc cgcttcgctg agcgaagcga ccggaaggcc
      841 gccgccggcg agtcgggaat cgatggcgtc cactccgaag ggaagacata agcgccgttc
      901 cgagccggcg gattcgagcg ccctgacttc cgcgcgcagg gcggccaggc gttcggctcg
      961 ggcggggctc cggggcattg cgactcacga ctcatgcgtt ccgcttatgt tcccacctcg
     1021 ttcagcgaga gtcaagttgg ggggaggcct atctgcctcc ccatcgcaga tggggagggg
     1081 gaccaggcga agcctggtgg aggggtagaa ggcgccacgg cttgaggttc tcgcgggagc
     1141 cactaagtcc ggcgccaact acccctccac caccttcggt ggtccccctc cccgtcgctt
     1201 cgcgacaggg aggcaaaagg aattccatgc tccgccgctg tctcctcgct ctcctcctcc
     1261 tcgtcgccgc gccggtccag gccgcgggcg attcgagctg gttctacaag aacaccgaca
     1321 tcccgcccga tccggcctgg acgttcggca cccttcccaa cggcctgcgc tacgcggttc
     1381 ggcgcaacgc ggtgccggag gggcaggtgt cggtgcgcct gaggatcgac gccggctccc
     1441 tccacgagga ggaaaaggag cggggctggg cgcatttcgt cgagcatatg gcggtccggg
     1501 gcacgaagaa tttcggcgac ggggaagttc gcgagacctg gcagcggctt ggcgccagct
     1561 tcggaagcga caccaacgcc tcgaccgaac cgacccagac cgtctacatg ctcgaccttc
     1621 cgagggccga ccgggcctcg ctcgacacca gcctgcgcct gctcgccgac atggccgaca
     1681 ccgccctttt cgacccgaag atcgtcgata ccgagcgcgg agtcgtgctc tccgaatatg
     1741 gccgccggac cgagctcgcg gtgaagctgc gggagatgat gatgccgctc ttctacggcg
     1801 gcctcaaata tgcgcagcgc gacacgatcg gaaccgaggc gactctgaag ggcgccgacg
     1861 cggccggcct caaggctttt tacgagcgct ggtaccggcc cgaccgggcg actttggtga
     1921 tggtcggcga cgccgatccc aggctcatgg aggagttgat cgcggcgcgc ttcggcggct
     1981 ggaagccctc cggaccggcg ccgagcgagc ccgattacgg ggcgcccgcc gacgtgccgc
     2041 agcgaacggc ggcgatcgcc tatccgggct ccccgcattt cgccaccctg agctggatcc
     2101 ggccctaccg gcgggtgccg ccgagcaagg cgcgcgagca gaggaacctc gcccgaaccc
     2161 tcgccagccg gatcctcaac cgtcggctcg aggccaaggc gcggggcgag gccgccttcc
     2221 tcaacgccca ggtgcaggcc gaaaacagcg tcaacgtcgc cgataccacc cagatgtcgg
     2281 tgatggcgaa ggagggacgc tggcagcagg ctctgaccga ggcctacgcg atcctcgccg
     2341 atgcgctgaa ggcgccgccc agccggaccg agatcgaccg cgagatggag aatatccgaa
     2401 ccgccggccg ggccgccgtc cagggcgatc cgacccagcg ctcgcagatc cgggccgggc
     2461 ggctcatctc ggcgatcgac ggcaacagcg tcatctccac cgcccagatc cagctcgctt
     2521 tgttcgagga gctggcaccg tcgatgactc cggaagcggt gcaggcggcg ctgaaggaca
     2581 tgttccaggg ctcggggccg aggctggcca tgctgtcccc ggcgccggtg gcgggccttg
     2641 cccaggcgct cgccgccgcc gaggccgcgg ccccggcggc gcgccaggcc gacaggc
//
LOCUS       DASWNU010000356         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_64635, whole genome shotgun sequence.
ACCESSION   DASWNU010000356 DASWNU010000000
VERSION     DASWNU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64635"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..676
                     /locus_tag="VGB57_07135"
     CDS             299..676
                     /locus_tag="VGB57_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131161.1"
                     /translation="MKSPIPFAAAPLAALLALAACGNETIDGNADPAANAAAANNAAT
                     IEAPPMIQASRTYRCKDNSLLYADFYTNDTVQVRAAKDQPPTVLTAAAGQPPFTAEGY
                     SLSANEAQISYTAPGKGTQTCRA"
     gene            complement(755..1420)
                     /locus_tag="VGB57_07140"
     CDS             complement(755..1420)
                     /locus_tag="VGB57_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0131162.1"
                     /translation="MPRPARIVLPGIAHHVVQRGNRRQRLFFDDGDRRAYLKLLAAAL
                     ERHSTSCLGWCLMDNHVHLILLPVSADGLRAPLASVHTAYAQRVNRARELGGHLFQGR
                     FASYAMDDAHLMVAARYVENNPVAAGMVGRAEAWPWSSATAHVEGRGDGLTDVAALGR
                     HVPDWKAMLARGLEAGDEAERVELSLRTGRPLGSREWLRALAATYGIAVDPPARGRPR
                     RAR"
     gene            1893..4115
                     /gene="purL"
                     /locus_tag="VGB57_07145"
     CDS             1893..4115
                     /gene="purL"
                     /locus_tag="VGB57_07145"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY0131163.1"
                     /translation="MSESITPQVVAEHGLSHEEYERVLHALGREPNMTELGIFSVMWS
                     EHCSYKSSRIHLKKLPTEADWVICGPGENAGVIDIGDGQAAIFKMESHNHPSYIEPYQ
                     GAATGVGGILRDVFTMGARPVANLNALRFGRPDHPKMRHLIAGVVAGIGGYGNCVGVP
                     TVGGEVNFHRAYDGNILVNAMTVGVADQDKIFYSAAAGIGNPVVYVGSKTGRDGIHGA
                     TMASADFSEDSEEKRPTVQVGDPFTEKLLIEACLELMASDAIVAIQDMGAAGLTSSSV
                     EMASKGGVGLELDMNAVPCRETGMTPYEMMLSESQERMLMVLKPGREPEAEAIFRKWE
                     LDFAVIGRVTGTGHLVLKWNGETAADIPLAPLADDAPCYDRPWVPTPKPEPLADIPEC
                     ADVAADLMRLMASPDLASRRWVWEQYDHMVGANTVQRPGGDAALVRVHGTDKALAVTT
                     DVTPRYCFADPVEGGKQAIAETYRNLCAVGATPLATTDCMNFGNPQRPEIMGQFVGCI
                     EGMAEACRALDFPIVSGNVSLYNETKAEDGSGSAILPTPAIGGVGLLKDWRKGVGIAF
                     KGIGDVIVLIGTRRGHLGQSLWLREIHGREDGPPPPVDLAAERCAGEFVRAAIAGGAI
                     TACHDVADGGLAVALAEMALAGDVGAMINKLRPQSNAALFAEDQGLYVATICDNCLLT
                     FLARAHAAGVEVEAIGRTCGTRLIFELPEGDHCIPLEDLRRAHEGFFPKLMGSDAGLA
                     "
BASE COUNT          779 a         1567 c         1410 g          656 t
ORIGIN      
        1 ccggaacgcc gacctggttg ccgccggaga gctgagtctc gatcagattg gcggtgatcg
       61 gcgcctcgtt gctcgggctg gtgaagaccg cgccgagcag cacgagcagg aacacgaagg
      121 cgaggccggt gaggccgata cggacccgtt gcacgcgctt tccgatctcc cggccccaca
      181 atcttgcgta aagggtaact tacgccgcgg cctttgtcga accccgccgt cgctgttgcg
      241 aagcactcgc agaaaagccc ttgacgagcc atgctgcgat gccgaagtga ttgatatgat
      301 gaaaagccca atccccttcg ccgccgctcc cctcgcggcc ctcctcgccc tcgccgcctg
      361 cgggaacgag accatcgacg gcaatgccga tcccgctgcc aacgccgccg cggccaacaa
      421 tgccgcgacg atcgaggcgc cgccgatgat acaggccagc cgcacctacc ggtgcaagga
      481 caacagcctg ctctacgccg atttctacac caacgacacg gtccaggtgc gagccgccaa
      541 ggaccagccg ccgaccgtcc tcaccgccgc cgcaggccag ccgcccttca ccgccgaagg
      601 ctattctctg agcgccaacg aagcccagat cagctacacc gcccccggca agggcaccca
      661 gacctgcagg gcgtaaaaca gggacagtcc ctactttcgt aaaacaggga cagtccgtgt
      721 ttagcggatg gggaccgtcc ctttttttgc aggctcagcg agcgcggcgg ggacgtccgc
      781 gagcgggcgg gtcgacggcg atgccatagg tcgcggccaa ggctcgaagc cactcccggc
      841 tgcccagtgg ccggccggtg cgcagggaaa gttcgacccg ctcggcctcg tcgcccgcct
      901 cgagccctcg cgccagcatc gccttccagt cgggcacgtg ccgtcccagc gcggcgacgt
      961 ccgtgaggcc gtcgccgcgg ccctcgacat gagccgtcgc gctcgaccag ggccaggcct
     1021 cggcccgccc gaccatcccg gcggcgaccg ggttgttctc gacatagcgc gccgcaacca
     1081 tcaggtgcgc gtcgtccatg gcgtagctgg cgaaccggcc ctggaacaaa tggccgccaa
     1141 gctcgcgggc tcgattcacg cgctgcgcat aggctgtgtg gacactggcg agcggcgccc
     1201 gcaggccgtc ggccgagacg ggcaggagga tcaggtggac gtgattgtcc atcaggcacc
     1261 agcccaggca cgaagtggag tgacgctcaa gggcggcggc gagcagcttc agataggccc
     1321 ggcgatcgcc gtcgtcgaag aacaggcgct gccgccggtt gccgcgctgg acgacgtggt
     1381 gggcgatccc cggaagcacg atcctggccg gccgcggcat gggccctcct caatcaggga
     1441 cagtccctct ttccgccggc tcccgcgaag agggtaaatg cctgcttttt cataaccaat
     1501 gtatttctta ccttatccac cgctttcgat caatcgcgcc gcacgactga gtggctgccg
     1561 cactgccaaa aaagaggacc gtccccgttt atacggaaaa gagggactgt ccctgtttat
     1621 acggggcgta agccaaaccc gtcaccctga gcgccaacga ggccagatca gcgacaccgc
     1681 ccccggcaag ggcacccaga cctgcaaggc gtaagccgca cccgccgccc cggcgaaagc
     1741 cagggcctcg gcggaccctc tgactccgtc accccggcga aagccggggc ctcggggaga
     1801 cccaccgaga cttctcgacc tggggtcccg gcttccgccg ggatgacggt tactcctgac
     1861 aagcctcccg cgcgccgcta acaggcgctc gcatgagcga atccatcact ccgcaagtcg
     1921 tcgccgagca cggcctctcc cacgaggaat atgagcgcgt cctccacgcg ctcggccgcg
     1981 agcccaacat gaccgagctc ggcatcttct cggtgatgtg gtccgagcat tgctcgtaca
     2041 aatcgtcgcg catccacctg aagaagctgc ccacggaggc ggactgggtg atttgcggcc
     2101 ccggcgagaa tgccggagtc atcgacatcg gcgacggcca ggccgccatc ttcaagatgg
     2161 agagccacaa ccacccctcc tacatcgagc cctatcaggg cgccgcgacc ggagtcggcg
     2221 ggatccttcg cgacgtcttc acgatgggcg cccgcccggt cgcgaacctc aacgcgctcc
     2281 gcttcggccg ccccgaccac ccgaagatgc gccacctgat cgcgggcgtg gtcgccggca
     2341 tcggcggtta cggcaattgc gtcggagtcc ccaccgtcgg gggcgaggtg aatttccacc
     2401 gggcctatga cggcaacatc ctggtcaacg cgatgaccgt cggggtcgcg gatcaggaca
     2461 agatcttcta ttcggcggcg gccggcatcg gcaatccggt cgtctatgtc ggctcgaaga
     2521 cgggccgcga cgggatccac ggcgcgacca tggccagcgc cgacttctcc gaggacagcg
     2581 aggagaagcg ccccaccgtc caggtcggcg acccgttcac cgagaaattg ctgatcgaag
     2641 cctgcctcga gctgatggcg tccgacgcga tcgtcgccat ccaggacatg ggcgccgccg
     2701 gcctcacctc ctcctcggtc gaaatggcgt cgaagggcgg agtcgggctc gagctcgaca
     2761 tgaacgccgt cccctgccgc gagaccggga tgacgcccta cgagatgatg ctctccgaat
     2821 cgcaggaacg gatgctgatg gtcctgaagc ccggccgcga acccgaggcc gaggccatct
     2881 tccgcaagtg ggagctggat ttcgcggtca tcggccgggt caccgggacc gggcacctgg
     2941 tcctcaaatg gaatggcgag accgcggccg acatcccgct cgcaccgctc gccgacgacg
     3001 cgccctgcta cgaccggccc tgggtgccta ctccgaagcc ggagccgctg gcggacatac
     3061 ccgaatgcgc cgacgtcgcc gccgacctga tgagactgat ggcctcgccc gacctcgcca
     3121 gccgccgctg ggtctgggag caatatgacc atatggtcgg cgcgaatacg gtgcagcgcc
     3181 cgggcggcga cgccgcattg gtgcgggttc acggcaccga caaggcgctc gccgtcacca
     3241 ccgacgtcac cccgcgctat tgcttcgccg acccggtgga aggcggcaag caggcgatcg
     3301 cggagacgta tcgcaacctc tgcgcggtcg gagccacccc gctcgcgacc accgactgca
     3361 tgaatttcgg caacccgcag cgtcccgaga tcatgggcca gttcgtcggt tgcatcgaag
     3421 gcatggccga agcgtgccgg gcgctcgact tcccgatcgt ctccggcaac gtctccctct
     3481 ataacgagac caaggcggag gacggcagcg gcagcgccat cctccccact ccggcgatcg
     3541 gcggagtcgg tcttctcaag gactggcgca agggcgtggg catcgccttc aagggcatcg
     3601 gcgacgtcat cgtcctgatc ggcacccgcc gcggccatct cggccagtcg ctttggcttc
     3661 gcgagatcca cggccgcgag gacggcccgc cgccgccggt cgacctcgcc gccgagcgct
     3721 gcgccggcga gttcgtccgg gcggccatcg ccggaggcgc gatcaccgcg tgccacgacg
     3781 tggccgacgg cggcctcgcc gtcgcgctcg ccgaaatggc tttggccggc gatgtcgggg
     3841 cgatgatcaa caagctgagg ccccagagca acgccgccct cttcgccgag gaccagggcc
     3901 tctacgtcgc caccatctgc gacaattgcc tcctcacttt cctcgcccgg gcccatgccg
     3961 ccggagtcga ggtcgaggcg atcggccgga cctgcggcac ccgcctgatc ttcgagcttc
     4021 cagagggcga ccactgcatc cctctcgagg acctgcgccg agcccatgaa ggcttcttcc
     4081 ccaagctgat ggggagcgac gcggggctcg cgtaagtcca ccccgggacc gtccacgtca
     4141 ttcccgcgaa agcgggaatc ccgctttctt tcctgcaccg ccctcgctcg gagaaggcac
     4201 gagcgcgtga cggcgcgacc gagtataggg agaagcggga ttcccgcatt cgcgggaatg
     4261 acgggagggg cccatgtcaa cggccggtta ctcaggcacg ccgctggcgc gaaagctgtc
     4321 gctgaaggac gggcagcgcg tctggtggga tggagtgccg acgagcgttc agtcggagat
     4381 cgagcgcgag ggcctggtcc tggacctcct cg
//
LOCUS       DASWNU010000357         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_298540, whole genome shotgun sequence.
ACCESSION   DASWNU010000357 DASWNU010000000
VERSION     DASWNU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298540"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VGB57_07150"
     CDS             complement(<1..379)
                     /locus_tag="VGB57_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0131164.1"
                     /translation="MAEEERDDLAELLARRALTEDAARPDAVEKRHAAGGRTARENVE
                     DLVDPGSFVEYGRYAIAAQRGRREVEDLIARTPADGLIAGTARVNGDLFDPERAACAV
                     LSYDYTVLAGTQGALGHRKKDRLF"
     gene            complement(398..643)
                     /locus_tag="VGB57_07155"
     CDS             complement(398..643)
                     /locus_tag="VGB57_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131165.1"
                     /translation="MPLWLARKAAPHVWKRVPWAKVWVAALWLAKKGQDRIQQNLTKH
                     EQTEFWNLVKKSKGRPDKLSNRDRTRVKNLVGKAIRG"
     gene            complement(656..1093)
                     /locus_tag="VGB57_07160"
     CDS             complement(656..1093)
                     /locus_tag="VGB57_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY0131166.1"
                     /translation="MSSKPLFAAALVALSLGLAACGDSGSDSTSTEAETAPPASETTT
                     PGGAETTESEPAPSGEAAKAEKVDIIEFTYQPDPVVIQAGGKVTWQNQDAAPHTATAD
                     DDSWDTGTIEKGKIGSETFKEAGKFAYFCEVHPDMRGTVEVVE"
     gene            1175..>1796
                     /locus_tag="VGB57_07165"
     CDS             1175..>1796
                     /locus_tag="VGB57_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0131167.1"
                     /translation="MITGPRGRWVTVAIWLLIGAAGLLAHAHIDDVTAAGQSSFLPAD
                     SESTRALDALQHASSGGEDVPVVIVFERRGGLSKADVAAIGRIGDGLGRLGLAGATPI
                     LDPFSGGYRSDLAKVARVANGIGPISRDGEAALLVLAIDADDRGAIVDGVEGIRGYLD
                     EHERPGLEAFVTGPGGIAADLEAVAADAGRTLLVATLGLVLLLLLAV"
BASE COUNT          217 a          634 c          641 g          304 t
ORIGIN      
        1 cgaagaggcg gtccttcttg cggtggccga gggcgccctg ggtcccggcc agcaccgtgt
       61 agtcgtagga gaggaccgcg caggcggcgc gctcaggatc gaagaggtcg ccgttgacgc
      121 gggcggtgcc ggcgatcagc ccgtcggcgg gggtgcgggc gatcaggtcc tcgacctccc
      181 tgcggccccg ctgggcggcg atcgcgtagc ggccgtactc gacgaaggag ccggggtcga
      241 ccaggtcctc gacgttctcc cgcgccgtcc tcccgcccgc cgcgtggcgc ttctcgaccg
      301 cgtcgggccg cgccgcgtcc tcggtcagcg cgcggcgggc aagcagctcc gccaggtcgt
      361 ctcgctcctc ctcggccacg ggaggatctt tacgcgatca gccgcggatc gccttgccga
      421 cgagattctt cacccgggtc cggtcccggt tgctgagctt gtccggccgg cccttcgact
      481 tcttcactag gttccaaaac tcggtctgct cgtgcttggt caggttctgc tggatccggt
      541 cctggccctt cttggccagc cacagcgccg ccacccagac tttcgcccag ggaacccgct
      601 tccagacgtg aggcgcggct ttgcgcgcta gccacaaagg catcgtcccg atcccctact
      661 cgactacttc gacggtgccg cgcatgtcgg ggtggacctc gcagaagtag gcgaacttgc
      721 cggcctcctt gaaggtttcc gagccgatct tgcctttctc gatcgtgccg gtgtcccagc
      781 tgtcgtcgtc ggcggtcgcc gtgtgcggag cggcgtcctg gttctgccag gtcaccttgc
      841 cgccggcctg gatcacgacc gggtcgggct ggtaggtgaa ttcgatgatg tcgacttttt
      901 cggccttggc cgcctcgccg ctcggggcgg gttcgctttc ggtcgtttcg gcgccgccgg
      961 gagtggtcgt ttcgctcgcc gggggagcgg tttcggcctc ggtcgaggtt gagtcggagc
     1021 cgctgtcccc gcaggcggca aggccgaggg agagggcgac cagggcagcg gcgaacagcg
     1081 gcttcgagct catatcgcct gcctacccac ctcggccgga gaaatcacct gcaccgccgg
     1141 ggaatcagtc ggcggggcga tctaggctcc agccgtgatc accggtcccc gcgggcgctg
     1201 ggtgacggtc gcgatctggc tcctgatcgg ggcggccggc ctgctcgccc acgcccacat
     1261 cgacgacgtc accgccgccg ggcagagcag cttcctgccc gccgactcgg aatcgacgcg
     1321 ggcgctcgac gccctccagc acgcctcctc cgggggggaa gacgtgccgg tggtaatcgt
     1381 cttcgaacgg cggggcgggc tgagcaaggc ggacgtcgcc gcgatcgggc ggatcggcga
     1441 cggcctcggc cggctcgggc tggcgggggc gacgccgatc ctcgatccct tctcgggggg
     1501 ctaccgcagc gacctggcga aggtggcgcg ggtcgccaac gggatcggac cgatctcccg
     1561 cgacggcgaa gcggcgctgc tggtgctggc gatcgacgcc gacgaccgcg gcgcgatcgt
     1621 cgacggcgtc gaggggatcc gcggctacct ggacgaacac gaacggcccg ggttggaggc
     1681 cttcgtcacc ggccccgggg ggatcgccgc cgatctcgag gcggtcgccg ccgacgccgg
     1741 ccgcaccctg ctcgtcgcca ccctcggcct cgtcctcctc ctgctgctgg ccgtct
//
LOCUS       DASWNU010000358         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_91647, whole genome shotgun sequence.
ACCESSION   DASWNU010000358 DASWNU010000000
VERSION     DASWNU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91647"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /gene="paaC"
                     /locus_tag="VGB57_07170"
     CDS             complement(<1..675)
                     /gene="paaC"
                     /locus_tag="VGB57_07170"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY0131168.1"
                     /translation="MASLPPIDLPSNATGAGAFDVPALEEGAALFEYLLRLGDDSLIL
                     GQRLGEWCGHAPSVEVDLRLANMALDLIGQATHFLDRAGTVEGKGRDGDSLAFRRDVL
                     DYRNCWLVEQPNGDFARTMARQFLFSTWQKLLFRELTGSKEELIAGVAEKAVKEVAYH
                     AELAAEWVIRLGDGTEESRRRMADGLDWHWRFMPELFEMDAIATEAARRGIGADVTLF
                     REDYHAR"
     gene            complement(822..1103)
                     /gene="paaB"
                     /locus_tag="VGB57_07175"
     CDS             complement(822..1103)
                     /gene="paaB"
                     /locus_tag="VGB57_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEY0131169.1"
                     /translation="MNDWPLWEVFVRSRGGMAHRHVGSVHAPDAEIALRHARDTYTRR
                     MEGISLWVVPSASIVASDPGQDEAMFDPAADKVYRHPTFYDIPEGITHI"
     gene            complement(1198..2181)
                     /gene="paaA"
                     /locus_tag="VGB57_07180"
     CDS             complement(1198..2181)
                     /gene="paaA"
                     /locus_tag="VGB57_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY0131170.1"
                     /translation="MYTTELGKSKNVASTEDAEKLAAFEARVAADEFIEPKDWMPEAY
                     RRTLTRQISQHAHSEIVGMLPEGNWITRAPSLRRKAILLAKVQDEAGHGLYLYSAAET
                     LGTSRREMIEALHSGKAKYSTIFNYPTLTWADIGAIGWLVDGAAIMNQVPLQRTSYGP
                     YARAMVRVCKEESFHQRQGYEICMAMMAGTADQQRMMQDALNRWWWPSLMMFGPPDAD
                     SPNTAQSMRWRIKRDTNDELRQKFVDITVPQAEAIGLRVPDPALKWNEEAGHYDFGEV
                     DWEEFYAVVRGEGPVAKERMTARRQAWEDGAWVREAAAAYAEKHSRKQEAA"
BASE COUNT          327 a          724 c          783 g          391 t
ORIGIN      
        1 gcgggcgtga taatcttcgc ggaacagggt cacgtccgcg ccgatgccgc ggcgcgccgc
       61 ttcggtcgcg atcgcgtcca tctcgaagag ctcgggcatg aagcgccagt gccagtcgag
      121 cccgtccgcc atccggcggc ggctctcctc agtgccgtcg cccaggcgga tcacccattc
      181 ggcggcgagc tcggcatgat aggcgacctc cttcacggcc ttttccgcga ctccggcgat
      241 gagctcctcc ttcgagccgg tgagctcacg gaaaagcagc ttctgccagg tcgagaacag
      301 gaattgccga gccatcgtcc gggcgaagtc gccgttgggc tgctcgacca gccagcaatt
      361 gcgatagtcg agcacgtcgc ggcggaaggc gagggagtcg ccgtcgcggc ccttgccctc
      421 gaccgtgccg gcacggtcga ggaagtgggt ggcctggccg atcaggtcca gcgccatgtt
      481 ggcgaggcgg aggtcgacct cgaccgacgg cgcgtggccg caccattcgc cgagccgctg
      541 gccgaggatc aggctgtcgt cgccgaggcg gaggagatat tcgaacaggg cggcgccttc
      601 ctccagcgcc gggacgtcga aggcgccagc gccggtggcg ttggagggga ggtcgatggg
      661 ggggagggag gccatggcct aaaatcctcc cctgcatttg caggggaggg ggaccagccg
      721 aaggctggtg gaggggctct tggacgccgg agagcccctc caccgcgctt cgcgcggtcc
      781 ccctccccgt gaaatctcgg ggaggaattt aacggccccc ctcatatgtg ggtgattcct
      841 tcggggatgt cgtagaaggt cgggtggcgg tagaccttgt cggcggcggg atcgaacatg
      901 gcttcgtcct ggcccgggtc cgaggcgacg atcgacgccg agggaacgac ccacaggctg
      961 ataccctcca tccggcgggt gtaggtgtcg cgggcgtggc ggagcgcgat ctccgcatcg
     1021 ggggcgtgga cggagccgac gtggcggtgg gccattccgc ccctggagcg gacgaagacc
     1081 tcccagagcg gccagtcgtt cattgcgcgt cgtcccggcg gaagccggga cctcgggaag
     1141 acccgccgag acttcttgtc ccggggtccc ggctttcgcc gggatgacgg gtgaatgtca
     1201 tgccgcctcc tgcttgcggc tgtgcttctc cgcataagcc gccgccgcct cgcggaccca
     1261 ggcgccgtcc tcccaggcct ggcgccttgc cgtcatccgc tccttcgcga ccgggccttc
     1321 gccccggacg acggcgtaga attcctccca gtcgacctcg ccgaaatcgt aatggccggc
     1381 ctcctcgttc cacttcaaag cggggtcggg gacccggagg ccgatcgcct cggcctgggg
     1441 gacggtgatg tcgacgaact tctggcgaag ctcgtcattg gtgtcgcgct tgatccgcca
     1501 gcgcatcgac tgggcggtgt tcgggctatc ggcgtcgggc gggccgaaca tcatcagcga
     1561 cggccaccac cagcggttca aggcgtcctg catcatccgc tgctggtccg ccgtcccggc
     1621 catcatcgcc atgcagatct catagccctg gcgctggtgg aagctctctt ccttgcagac
     1681 ccggaccatg gcccgggcat aggggccgta ggaagtgcgc tggagcggca cctggttcat
     1741 gatcgcggcg ccgtcgacca gccagccgat cgccccgatg tccgcccagg tcagggtcgg
     1801 gtagttgaaa atggtgctgt acttggcctt gccgctgtgg agggcctcga tcatctcgcg
     1861 gcggctggtg ccgagagtct cggcggccga atagaggtag aggccgtggc ccgcctcatc
     1921 ctgcaccttg gcgagcagga tcgccttccg ccggagcgaa ggtgcgcgag tgatccaatt
     1981 gccttcgggg agcatcccga cgatctcgct atgggcgtgc tgcgagatct gccgggtgag
     2041 ggtgcgccgg taagcttcgg gcatccagtc cttgggctcg atgaactcgt cggcggcgac
     2101 ccgcgcctcg aaggcggcga gcttctccgc atcctcggtg gaagcgacgt tcttcgactt
     2161 gccgagctcg gtcgtgtaca tgctgttctc ctggcgcggg ccctagcagg atgcaccttt
     2221 tgcgg
//
LOCUS       DASWNU010000359         5925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_118257, whole genome shotgun sequence.
ACCESSION   DASWNU010000359 DASWNU010000000
VERSION     DASWNU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118257"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VGB57_07185"
     CDS             complement(<1..1253)
                     /locus_tag="VGB57_07185"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEY0131171.1"
                     /translation="MKLVVVESPAKAKTIEKYLGTGYRVLASYGHVRDLPPKDGSVDP
                     DQGFAMEWETYADKARQLKAITDEAKKADTLILATDPDREGEAISWHVQEVLRKRKAL
                     PADVERVTFNAITKATVTEAMKHPRALDEDLIDAYRARRALDYLVGFTLSPVLWRKLP
                     GAKSAGRVQSVALRLVVDREREIELFRAQEYWSVAALMESDGQEFLARLVRFKGEKLD
                     RLSIGDSGTAQAAKAAVEAGRFSVASVETKPLTRNPPPPFTTSTLQQEAARKLGFAAS
                     HTMRIAQALYEDGSITYMRTDGVQMAPEAISEARRAVANRFDAGYVPDKPRHYETKAK
                     NAQEAHEAIRPTDFGRDRAGSGDHGRQGGGGGGALHRRVGRDQAADAQPAATLHHLDP
                     PAGGGAQARLRRQSHDADRPGAVRGR"
     gene            complement(1312..1962)
                     /locus_tag="VGB57_07190"
     CDS             complement(1312..1962)
                     /locus_tag="VGB57_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131172.1"
                     /translation="MMPERESAGYLLAETVHLLRVNAGIALAALGAMTLLGMVSDVYP
                     DFAALAALASIPVNLVLQYEISRALLVHYDLVDGRARRRRLWALLGLNLLSGIAILFG
                     LLFLIVPGVYLLVRWWAAAPALIAEEADVTGSLDLSAEAVKGRFWHVFAAVSVVVWAP
                     AAAGVLASALVPEGEPLIASLLLNLSLNLCLIAGWHLAVAIYAGRQNYRNLAEVFA"
     gene            complement(2152..3738)
                     /locus_tag="VGB57_07195"
     CDS             complement(2152..3738)
                     /locus_tag="VGB57_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0131173.1"
                     /translation="MRHAAQLGIVSFSAIGLCLAAAGSFAPGAAAQGREPLCRRAAPL
                     PPWPPEARLNDQQQQRYRGHPAPPVLVEPAKGFGPAIAAPPAAPAPPEPMPAPPPPPP
                     PPPAAMEVGAESGEDSISVTGSRIGESRSATSLPSSAGDAVAESRIAHGFHRPPRPRP
                     PSGLLTAGDHDDLLNPELYATYAGNYLQRSNLRDLPRLDTRRVLTVAVQDGAGRPVPF
                     ARVTLTCADGNTLSLATVADGTAVFHPGLDRLGSSVRLSVPGAPGRRIEIAEGSGSQR
                     QSVTVRSGAVPVRKFDLLLAIDTTGSMGDEINYLKSELRTILTDLRRSHPGLDIRLGL
                     VAYKDEGDVYVTRTYPFVSDVNVMQANLQAQYAGGGGDYPEAMDLALARAVSQDWRAD
                     AVKSLLLVADAPPHDENIGKAWRAAEAARAKRIQIVPVAASGVGDVAEYAMRAMAAAT
                     QSRYIFLTDDSGIGNPHATPAVDCFLVTRLDRLVRRVLDSQISGRRVEPREGEVIRSV
                     GRYEGGTCILPPDYRRWEQY"
     gene            complement(3961..5052)
                     /gene="dprA"
                     /locus_tag="VGB57_07200"
     CDS             complement(3961..5052)
                     /gene="dprA"
                     /locus_tag="VGB57_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY0131174.1"
                     /translation="MPEASGDQIARLRLIRSENIGPITYFQLLARFGSAAAALEAIPD
                     LAARGGGRAPRLASRSAIEREMEQVERIGARYLFLGQGLYPPLLAELETAPPALIVKG
                     HPSLLDRPTAAMVGARNASAAACRFARSLAQALGAAGTVIVSGLARGIDSAAHDGSLD
                     SGTIAVVAGGIDIFYPPENEARQRAIAERGLVVSEQPPGVEPRSRHFPSRNRIIAGLA
                     YGTVVVEAAPRSGSLITARCAAECGREVMAVPGSPLDPRAQGCNQLIREGATLVQSAD
                     DVLEAIGPSRVRPFRQPERDYGIPEVVVEADDEAREAVIQMLNGTPVPVNELIRQAGL
                     PPAVVQTVLLELELAGRLERHAGGKVSLM"
     gene            complement(5045..5656)
                     /gene="plsY"
                     /locus_tag="VGB57_07205"
     CDS             complement(5045..5656)
                     /gene="plsY"
                     /locus_tag="VGB57_07205"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY0131175.1"
                     /translation="MQPIVEPILALLLGYALGSIPFGILLTGLTGAGDLRKIGSGNIG
                     ATNVLRTGRKGLAAATLLLDLAKGTAALLLAERLFPGTVQLAAIGAMVGHLWPVWLRF
                     AGGKGVATYVGVALGFVLTDGLAPWVPAAYAASWIGALLLTRYSSLGGLAAALVTPVA
                     AALAGRFDLALLFLGFTLLVFWKHRENIARLFAGTESRVGQGA"
     gene            5770..>5925
                     /locus_tag="VGB57_07210"
     CDS             5770..>5925
                     /locus_tag="VGB57_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY0131176.1"
                     /translation="MTSHRPLLVFDSGVGGLSVLGAIRSLLPAAPLVYAADSAGYPYG
                     TKRPAEIE"
BASE COUNT          949 a         1994 c         2129 g          853 t
ORIGIN      
        1 cgtcctcgta cagcgcctgg gcgatccgca tcgtgtgact ggcggcgaag cctagcttgc
       61 gcgccgcctc ctgctggagg gtcgaggtgg tgaagggtgg cggcgggttg cgcgtcagcg
      121 gcctggtctc gaccgacgcg acggtgaagc gccccgcctc caccgccgcc ttggcggcca
      181 tggtcgcccg agccggcccg gtcgcggccg aaatcggtcg ggcggatcgc ctcatgggcc
      241 tcctgggcgt tcttggcctt ggtctcgtaa tggcgcggct tgtccgggac gtagccggcg
      301 tcgaaacggt tcgccacggc gcggcgcgcc tccgagatcg cctcgggcgc catctggacc
      361 ccgtccgtcc gcatgtaggt gatcgagccg tcctcgtaca gcgcctgggc gatccgcatc
      421 gtgtgactgg cggcgaagcc tagcttgcgc gccgcctcct gctggagggt cgaggtggtg
      481 aagggcggcg gcgggttgcg ggtgagcggc ttggtctcga ccgaagcgac cgagaagcgg
      541 ccggcctcga ccgcggcctt ggcggcctgg gcggtgccgg aatcgccgat cgaaagacgg
      601 tcgagctttt cgcccttgaa ccggaccagc cgggcgagga actcctgccc gtccgactcc
      661 atcaaggcgg cgacgctcca atattcctgg gcccggaaca gctcgatctc gcgctcgcgg
      721 tcgacgacca ggcgaagcgc caccgactgc acccggccgg cggacttggc gcccggcagc
      781 ttgcgccaaa gcaccgggga gagggtgaag ccgaccaaat agtcgagggc gcggcgagcg
      841 cggtaggcgt cgatcagatc ctcatcgagg gcgcggggat gcttcatcgc ctcggtgacg
      901 gtcgccttgg tgatggcgtt gaaggtgacc cgctcgacgt cggcggggag cgccttgcgc
      961 ttacgaagca cctcctggac gtgccagctg atcgcctccc cttcccggtc cggatcggtg
     1021 gcgaggatca gggtgtccgc cttcttcgcc tcgtcggtga tcgccttcag ctggcgggcc
     1081 ttgtcggcat aggtctccca ttccatggcg aagccctggt ccggatcgac cgagccgtcc
     1141 ttgggcggaa gatcgcggac atggccgtag gaggcgagca cccggtagcc ggtgcccaga
     1201 tatttctcga tggttttcgc cttggcgggc gattcgacga cgacaagctt catggggatc
     1261 cttcacgcgt acgcgtatag ggtggggacc cttggtaagc gtcgtcaagc gctacgcgaa
     1321 cacctccgcc aggttccggt agttctggcg tccggcgtag atcgccaccg caaggtgcca
     1381 gccggcaatc aggcagagat tgagagaaag gttcaagagc aggctcgcga tcagaggctc
     1441 cccctcgggc accagcgcgg aggccagcac ccccgctgcc gcaggcgccc aaaccaccac
     1501 cgataccgcc gcgaagacat gccagaaccg ccctttgacc gcctccgcgc tgaggtccag
     1561 cgagccggtt acgtcggcct cctccgcgat cagggccgga gccgcggccc accagcgcac
     1621 gaggaggtac acgccgggga cgatgagaaa gagcaggccg aacaaaatcg cgattccgct
     1681 cagcaggttc agccccagca acgcccacag ccgccgccgg cgggcgcggc cgtcgacgag
     1741 gtcgtaatgg acgagcagcg cccggcttat ttcatattga agaacgaggt tcacagggat
     1801 ggaagcgaga gctgccagcg cggcgaaatc gggatacaca tcggaaacca tgcccagcag
     1861 ggtcatggcg cccagcgccg ccagcgcgat accggcattg acgcgcagca aatgcacggt
     1921 ttccgccaac aggtaacccg cgctttcccg ctccggcatc atggcgaccc atccagcatt
     1981 ccgcatactc gccccgccgg cgcgtaagcg tacagggtgg ggaccccggt ggagccgcgt
     2041 caagcgctct cgtcaccagc cgtcgtccca gcgaaagccg ggatcccagg gggtgaaagg
     2101 cgccgtggtc ttcgcccgct ggggccccgg ccttcgccgg gcgacggaaa ctcaatattg
     2161 ctcccagcgc cggtagtccg gcgggaggat gcaggtgccg ccttcgtagc ggccgaccga
     2221 gcggatcacc tcgccttccc tcggctcgac ccggcggccg gagatctggc tgtcgaggac
     2281 ccggcggacg aggcggtcga ggcgggtgac gaggaagcag tcgacggcgg gggtggcgtg
     2341 aggattgccg atgccgctgt cgtcggtgag aaagatgtag cgcgactggg tcgccgcggc
     2401 catcgcccgc atcgcatatt cggcgacgtc gccgactccc gacgcggcga cggggacgat
     2461 ctggatccgc ttggcgcggg cggcctcggc ggcgcgccag gcctttccga tattctcgtc
     2521 gtgcggcgga gcgtcggcga cgaggagcag cgacttgacc gcgtcggctc gccaatcctg
     2581 ggagaccgcc cgggcgagcg ccagatccat cgcctccgga tagtcgccgc cgcctccggc
     2641 atattgggcc tgcagattgg cctgcatgac gttgacgtcc gagacgaacg gataggtacg
     2701 ggtgacgtag acgtcccctt cgtccttgta ggcgacgagg ccgagccgga tgtcgagccc
     2761 gggatggctt cgcctgaggt cggtcaggat cgttcgaagc tcggatttca gatagttgat
     2821 ctcgtcgccc atgcttccgg tggtgtcgat cgccagcagc aggtcgaact tgcgcaccgg
     2881 aacggcgccg gagcggacgg tgacgctctg ccgctgcgag cctgagccct cggcgatctc
     2941 gatccggcgg cccggcgctc ccggaacgct gagacggacg ctgcttccga gccggtcgag
     3001 accgggatgg aagaccgcgg tgccgtcggc gacggtggcg agcgagagcg tgttgccgtc
     3061 ggcgcaggtc agggtcactc gcgcgaaggg cacgggccgt cccgcgccat cctgaacggc
     3121 gaccgtcagc acccggcgcg tgtccagcct cggcaggtcg cgaagattgc tgcgctggag
     3181 gtaattgcca gcataggtgg cgtagagctc cggattgagc aaatcgtcat ggtcgccggc
     3241 ggtcagcaga cccgatggcg ggcggggccg cggcggacgg tgaaatccgt gcgcgattcg
     3301 cgattcggcg acagcgtcgc cggcgctcga cgggagcgat gtcgccgacc ggctctcgcc
     3361 gatacggctg ccggtgacgg aaatactatc ctcgccgctc tcggcgccaa cctccatggc
     3421 cgccggaggc ggcggcgggg gcggaggcgg cgccggcatc ggctccggcg gggccggggc
     3481 agcgggcgga gccgcaatgg ccggcccgaa gcccttggcc ggctcgacca ggacgggggg
     3541 agcggggtgg ccgcggtagc gctgctgctg ctggtcgttc aacctcgcct cgggcggcca
     3601 gggcggcagg ggcgccgctc gccggcagag cggctcccgg ccctgcgccg cggcgcccgg
     3661 agcgaagctg ccggcggcgg cgaggcaaag accgatggcg ctgaagctga cgatcccgag
     3721 ctgggcggcg tgacgcatga cattctcccc tgtcgcgaat gttacaacgt ctcataaaac
     3781 agcagcgggc acctgaacgg aagctgacgc ggtttgcgcc agaaattcct ccccgcgatt
     3841 tcgcggggag ggggaccgcg cgaagcgcgg tggaggggtt ctttggcgtc agaaagaacc
     3901 cctccaccag gcttcgcctg gtccccctcc ccggcaaagg ccggggagga ttttgtgccc
     3961 tcacatcaag ctcacctttc ctccggcgtg gcgctccagc cgccccgcca gctccagctc
     4021 gagcagcacg gtctggacga cggcgggcgg caagcctgcc tggcggatga gctcgttgac
     4081 cggaaccgga gtgccgttga gcatctggat gaccgcctct cgcgcttcgt cgtcggcttc
     4141 cacgaccacc tccggtatcc cgtaatcccg ttcgggctgg cggaaagggc ggacccgcga
     4201 ggggccgatc gcctccagca cgtcgtcggc cgactggacg agggtggcgc cctcgcggat
     4261 cagctggttg cagccctggg cgcgggggtc gagcggcgag cccggcaccg ccatcacctc
     4321 gcggccgcac tcggcggcgc agcgcgcggt gatcagcgag cccgagcggg gcgccgcctc
     4381 gaccaccacc gtcccgtaag cgagtccggc gatgatccgg ttgcgcgagg ggaaatggcg
     4441 ggagcgcggc tcgacgcccg gcggctgctc ggaaacgacc aggccgcgtt cggcgatcgc
     4501 ccgctggcga gcctcgttct cgggcgggta gaagatgtcg atcccgccgg cgacgacggc
     4561 gatcgtgccg ctgtccagcg acccgtcatg cgccgccgag tcgatccccc gggcgaggcc
     4621 ggagacgatg accgtgcccg ccgcgccgag cgcctgggcc agcgagcgcg cgaagcggca
     4681 ggcggcggcc gaggcgttgc gcgctccgac catggccgcc gtcggcctgt cgagcaggct
     4741 tggatggccc ttgacgatca aagcgggcgg tgccgtctcc agctcggcca gaagcggagg
     4801 atagaggccc tggccgagga acaggtagcg ggcgccgatc cgctccacct gctccatctc
     4861 ccgctcgatc gcggatcggg acgcaagtcg gggcgcccgg ccgccgccgc gggcggccag
     4921 atcggggatc gcctcgagcg ccgcggcggc cgagccgaag cgggcgagca gctggaaata
     4981 ggtgatcgga ccgatattct cggagcggat cagccgcagc cgggcgatct gatcgcccga
     5041 cgcctcaggc accctggccg acccgcgact cggtcccggc gaagaggcgc gcgatattct
     5101 cccgatgctt ccagaaaacg aggagggtga agccgaggaa gagcaaggcg aggtcgaagc
     5161 ggccggccaa cgccgccgcg accggcgtga cgagagccgc ggccagcccg ccgagcgagg
     5221 aatagcgggt caggagcaga gcgccgatcc agctggcggc gtaagcggcg ggcacccagg
     5281 gggcgagtcc gtcggtgagc acgaaaccga gcgccacgcc gacataggtg gccaccccct
     5341 tccctcccgc gaagcgaagc cagaccggcc agaggtggcc gaccatcgct ccgatcgcgg
     5401 cgagctggac ggtgccggga aacagccgct cggccagaag cagagcggcg gtgcccttgg
     5461 ccaggtcgag caggagggtg gcggcggcga ggcccttgcg gcccgtcctc aggacgttgg
     5521 tcgcgccgat attgcccgag ccgatcttgc gaaggtcgcc ggcgccggtc agcccggtga
     5581 ggaggattcc gaaggggatc gaccccagcg cgtagccgag gagcaaagcc aggataggtt
     5641 caacgatcgg ttgcatgtcc acgacccctg cgcaaacttt agcaaatcgc aaagcggaag
     5701 caatactttc atcgaaagcg atgaaccgcg ccgccttgcc gccgtccgct ctgccgctaa
     5761 accgggcgcg tgaccagcca tcgtcccctc ctcgtcttcg attccggagt cggcggcctc
     5821 tccgtcctcg gagcgatccg gtcgctgctg cccgccgcgc ccctggtcta cgccgccgac
     5881 agcgccggct atccctacgg cacaaagcgg cccgcggaga tcgag
//
LOCUS       DASWNU010000360         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_39785, whole genome shotgun sequence.
ACCESSION   DASWNU010000360 DASWNU010000000
VERSION     DASWNU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39785"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGB57_07215"
     CDS             <1..294
                     /locus_tag="VGB57_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0131177.1"
                     /translation="AREAEERRGAEGYKQANHYGWGGPGDGSRSRDEMRALETLEIED
                     GDAPFESIKAAWRRLAKANHPDAAPGDADAAKRFQAVQAAYEVLRKSEERRAG"
     gene            413..1072
                     /locus_tag="VGB57_07220"
     CDS             413..1072
                     /locus_tag="VGB57_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131178.1"
                     /translation="MIFRTVALASFALLTACGIESEASAQAAAAASSPQAWIIGPIVR
                     GRNYSKGVPLHPTARRGGGWHIDIPPAPGSVHAATFRHGPLAGKRRIVMRYRIETARG
                     ARLLASSDGRSPSMITLYFQRRGDNWSGRGRFETYRWYATFATRSPLTAGDHVMIAPL
                     DGPWTAVETSKATTNPAAFRAALADADQVGFVLGGGDGFGHGISATGPARLIVTDFRV
                     E"
     gene            complement(1184..1621)
                     /locus_tag="VGB57_07225"
     CDS             complement(1184..1621)
                     /locus_tag="VGB57_07225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0131179.1"
                     /translation="MSRFGTDGGFDPELFTEFARKVGHGGALGILYVGHGADWVELGL
                     DWQEKLVGVPETGVLASGPIVSLMDMATSMAIWVKLGRFRPLATLDMRVDYLRPATPR
                     KRIVGRGECYGTTRSVGFVRGLAHDGDPADPVAHVTGTFMFTA"
     gene            complement(1618..2031)
                     /locus_tag="VGB57_07230"
     CDS             complement(1618..2031)
                     /locus_tag="VGB57_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY0131180.1"
                     /translation="MPSPSLAEVQADYELLEADDRYRLLIDLGRALEPMPDALKTDAT
                     LVRGCSASVWVYPVQRPDGRLHFLADSNAAITRGIVALVLLAVQDRRPEEIVATDVES
                     VLAPFDLKNQLSSNRTQGIPNMIALVRETAARLAQ"
BASE COUNT          373 a          820 c          808 g          344 t
ORIGIN      
        1 gcccgcgagg ccgaggagag gcgcggggcc gaaggctaca agcaggccaa tcattatggc
       61 tggggcggtc ccggcgacgg aagccgaagc cgcgacgaga tgcgcgctct ggaaaccctg
      121 gagatcgagg atggcgacgc gcccttcgaa tcgatcaagg ccgcctggcg ccgccttgcc
      181 aaggccaacc atcccgacgc cgctccgggc gacgccgacg cggcgaagcg cttccaggcg
      241 gtccaggccg cctatgaggt gctgaggaag tccgaggaga ggcgggcggg ctgaaaaatc
      301 ctcccctgct tttgcagggg agggggacca cccttcgggt ggtccccctc cccgagaaat
      361 ctcggggagg aattcagttc gtggacatga aggaacccgc atcatgcgat ccatgatctt
      421 ccgtaccgta gccctcgcgt ccttcgccct cctcaccgcc tgtgggatcg agtccgaagc
      481 cagcgcccag gccgccgccg ccgcgtccag tccgcaggcc tggatcatcg gcccgatcgt
      541 ccgcggccgc aattactcga aaggcgtgcc gcttcatccc accgctcggc ggggcggcgg
      601 ctggcatatc gacatcccgc cggcgcccgg cagcgtccat gccgcgacct tccgccacgg
      661 cccgctcgcc ggcaagcgcc ggatcgtcat gcgctaccgg atcgagaccg cccgcggcgc
      721 ccgcctgctg gcatcgagcg acggccgctc gccgtcgatg atcaccctct atttccagcg
      781 ccgcggcgac aattggagcg ggcgcggccg cttcgagacc taccgctggt atgcgacctt
      841 cgccacccga tcgccgctga ccgcgggcga ccatgtcatg atcgccccgc tcgacggccc
      901 ctggacggcc gtcgaaacca gcaaggcgac caccaacccc gccgccttcc gcgcggctct
      961 ggccgacgcc gaccaggtcg gcttcgtcct gggcggcggc gacggcttcg gccatggaat
     1021 ttccgccacc ggtccggccc gactgatcgt taccgacttc cgggtggagt gacttaccgt
     1081 catcccggcg aaagccggga cctcggggag acccgcggag acttcttgag atggcgaaaa
     1141 gagggactgt ccctctttat gccaaaagag ggactgtccc tctttatgcc gtgaacatga
     1201 aggtgcccgt cacgtgggcg acggggtcgg cgggatcgcc gtcatgggcg aggccgcgca
     1261 cgaagccgac gcttcgggtg gtgccgtagc attcgccgcg gccgacgata cgcttgcggg
     1321 gagtcgccgg gcgaagatag tcgacccgca tgtcgagagt cgccagaggc ctgaagcggc
     1381 cgagcttgac ccagatcgcc atgctcgtcg ccatgtccat caggctgacg atcggcccgg
     1441 aggcgagaac gcccgtctcc ggcactccga ccagtttctc ctgccagtcg aggccgagct
     1501 cgacccagtc ggctccgtgg ccgacgtaga ggatcccgag cgcgccgcca tggccgacct
     1561 tgcgggcgaa ctcggtgaac agctcgggat cgaagccgcc gtcggtgccg aagcggctca
     1621 ttgcgccagc cgggcggccg tctcgcggac gagggcgatc atgttgggga tgccctgggt
     1681 gcggttggag ctgagctggt tcttgaggtc gaacggcgcc agcaccgatt cgacgtcggt
     1741 cgcgacgatc tcctccggcc tccggtcctg aacggcgaga aggacgagcg cgacgatgcc
     1801 cctggtgatc gcggcgttgc tgtcggcgag gaaatggagc cgcccgtccg gccgctgcac
     1861 cggatagacc cagaccgagg ccgagcagcc gcgcaccagg gtggcgtcgg tcttgagcgc
     1921 gtccggcatc ggctcgagcg cccggccgag gtcgatcaga agccggtagc ggtcgtccgc
     1981 ctcgagcagc tcgtaatcgg cctggacctc ggcgagggaa ggggagggca tggggggccg
     2041 ctaccagagg cgggggggcg gcgcaaagcc tggccggttc aaattcctcc cccggttttc
     2101 ggggggaggg ggaccgcgcg cagcgcggtg gaggggttct tcgggctcgg caagaacccc
     2161 tccaccatgc ttcgcatggt ccccctcccc gagaaatccc ggggaggaat taaacgtcgc
     2221 ccccgctcgc gatcgcttcg agccgccgga cgcgagcgcg aagctcggcc agctccggcc
     2281 gcccgcccga gcccttgccg ccgccgccgc gcctggcgag ctcggttcgc ctcagctcca
     2341 gccag
//
LOCUS       DASWNU010000361         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_118333, whole genome shotgun sequence.
ACCESSION   DASWNU010000361 DASWNU010000000
VERSION     DASWNU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118333"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VGB57_07235"
     CDS             complement(<1..397)
                     /locus_tag="VGB57_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0131181.1"
                     /translation="MREVGLRIVTTGSCRHPEIATIEGGSWRPATFPALVALIVHPVE
                     GTILFDTGYDPAFLEATRRFPERLYRWLTPPEISSPVADRLAAEGVDATEVRHIVLSH
                     FHGDHVSGLSRFPNATVHCSKAGLSGVWGR"
     gene            complement(394..1347)
                     /locus_tag="VGB57_07240"
     CDS             complement(394..1347)
                     /locus_tag="VGB57_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0131182.1"
                     /translation="MVKIVVTGATGGIGRTLVPLVESLGLDVVASGRNAEAGRALEGP
                     RVRFLPLDLVDDDIAPLVAGAQVVLHLAARSSPWGPAAGFHADNVVATSRLVEAAARA
                     GVERFVHASTPAIFSERRHRLGLRNNDSPAARPANAYARTKLESERIVTGERRMATIV
                     LRPSAVLGPDDKSILPRLMRVLARGVLPVGNGGIALFHPTDVRDAARAFAEAALGTCT
                     GAANVAGREPVGIVEMAGALAQRLGVTLRVMRVPEPALHALAGAAELAGRAAGREPPL
                     TRYSVANLSWSRSFDLRETEALLGWSPAFGPLDALAHAVPR"
     gene            complement(1341..2333)
                     /locus_tag="VGB57_07245"
     CDS             complement(1341..2333)
                     /locus_tag="VGB57_07245"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY0131183.1"
                     /translation="MSNPTSFTLAGAGTFEPRNAVTSDVLDRRHGRASGTTLKLGISE
                     RRYASADETSSFMAAEAARRAVADAGWELGEVDALIGACGVMEQPIPGTAPLVQRRLG
                     LGESGIPAFDVNATCLSFLPALDHAVAGMAIKGWRRVLIFAADIASAALDHGDLEASA
                     IFGDGAAAVALEAGGTSRILASRLETYGDHSDLCRLEAGGTRLRPHDDLPGFLDRSRF
                     RMEGIALFKVTARRFPPFMARLFADAGLGPDDIGSIIPHQASGPALAHLGRALGGDPN
                     RIVDTFATLGNRIASSLPHTLAVARRENRLVPGSNSLLVGSSAGVSLGGTIIRW"
     gene            2424..>2756
                     /locus_tag="VGB57_07250"
     CDS             2424..>2756
                     /locus_tag="VGB57_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0131184.1"
                     /translation="MARRSDHSPEELKGMILAEGHQHLAEVGYSRFSAREVARRVGYA
                     VGTLYHVFGTLDRLMFEINTITFRQWADLLEHRLAEADGRRLDALVEGYFDFAIANRN
                     LWGAIWAHN"
BASE COUNT          463 a          941 c          963 g          389 t
ORIGIN      
        1 cccggcccca gacgccgctc agcccggcct tcgagcaatg caccgtcgca ttggggaagc
       61 gcgagaggcc cgaaacatgg tcgccgtgga aatgggaaag cacgatatgc cggacctcgg
      121 tcgcatccac gccctcggcg gccaggcggt ccgcgaccgg agacgagatt tcgggcggag
      181 tcagccagcg gtacaggcgc tcgggaaagc gccgggtcgc ttcgaggaac gccgggtcgt
      241 agccggtgtc gaacaggatc gtcccctcca cggggtgcac gatcagcgcc accagggccg
      301 ggaaggtcgc cggccgccat gagccgccct cgatggtggc gatctcggga tggcggcacg
      361 aaccggtcgt gacgatgcgc aggccgacct ccctcatcgc ggaaccgcgt gggcgagagc
      421 gtcgagcggg ccaaaggcgg gactccagcc gagcagcgct tccgtctccc gaaggtcgaa
      481 gcttcgcgac caggagagat tggccaccga atagcgggtg agcggcggct cgcggccggc
      541 ggctctgccc gcgagctccg ccgccccggc gagggcgtgc agggccggct ccgggacgcg
      601 cattacgcgc agcgtcacac ccagtcgttg cgcgagcgct cccgccatct cgacgatgcc
      661 taccggctcg cggccggcga cgttggcggc accggtgcag gtgccgagcg cggcctcggc
      721 gaaggcccgg gcagcgtcgc gcacatccgt cggatggaac agggcgattc cgccgtttcc
      781 gaccggaagc actccgcgcg caagcacccg catgagcctt ggaaggatcg acttgtcgtc
      841 ggggccgagg accgccgacg gccggagaac gatcgtcgcc atccggcgct cgcccgtgac
      901 gatccgctcc gactccagct tggtccgggc ataggcgttg gccgggcgcg ccgcgggact
      961 gtcgttgttg cgaaggccga ggcggtgccg gcgctcgctg aagatggcgg gggtcgaggc
     1021 gtggacgaag cgctccaccc cggcccgggc ggcggcctcg accagcctcg aggtcgcgac
     1081 gacattgtcg gcgtggaagc ccgcggccgg tccccacggg gacgagcggg ccgccaggtg
     1141 aagcacgacc tgcgcgccgg cgaccaaagg cgcaatgtcg tcgtcgacca gatccagcgg
     1201 caggaagcgg actcgcggac cttcgagagc tcggccggcc tcggcattgc ggccggacgc
     1261 gacgacatcc aggccgagcg actcgaccag cggcacgagc gtccggccga tccctccggt
     1321 ggcgccggtg acgacgatct tcaccaccgg atgatcgtcc cgcccagcga cacccccgcc
     1381 gaagagccga cgaggaggga attcgagccc ggaaccagcc gattctcgcg gcgagccacc
     1441 gcaagggtgt gcggaaggct cgaggcgatc ctgttgccca gcgtcgcgaa ggtatcgacg
     1501 atccggttcg ggtcgccccc gagcgcccgg ccgagatgcg ccagcgccgg ccccgaagcc
     1561 tggtgcggga tgatgctgcc gatatcgtcc gggcccaggc cggcgtccgc gaacagccgg
     1621 gccatgaagg gcgggaagcg ccgggccgtc accttgaaga gcgcgatacc ctccatccgg
     1681 aaccggctcc ggtcgaggaa gccgggcaga tcgtcgtgcg gcctcagccg ggtgccgccg
     1741 gcttccagcc tgcacaggtc gctgtgatcg ccataggtct ccagtcgcga agcgaggatc
     1801 cgcgaagtgc ctccggcctc cagcgccacg gcggcggccc cgtctccgaa aatggcggaa
     1861 gcttcgaggt cgccgtgatc gagcgccgcg gaggcgatgt ccgcggcgaa gatcagaact
     1921 cgccgccagc ctttgatcgc catcccggcg acggcatgat cgagggccgg caggaaggag
     1981 aggcaggtgg cgttgacgtc gaaggccggg attccggatt cgcccagccc gagacggcgc
     2041 tggacgagcg gcgccgtccc cggaatgggc tgctccatca ccccgcaggc gccgatcagc
     2101 gcgtccacct ctcccagctc ccagcccgcg tcggcgacgg cgcgccgtgc ggcctcggcg
     2161 gccatgaagc tgctcgtttc gtcggccgag gcgtagcgcc tctcgctgat cccgagcttc
     2221 agggtggttc cggacgcccg gccatgtcgc cggtccagga cgtcggaggt gaccgcgttc
     2281 ctgggctcga aggttccggc acctgccagc gtgaagctgg tcggattgct catgcgctgt
     2341 tccatgagaa ggatcgtatc atacaattat acactgtcca ataacaattt tacattgtct
     2401 aataagcctg ctacccatta atcatggcac ggcgaagcga ccacagcccc gaggagctca
     2461 agggcatgat cctcgccgag ggacatcagc atcttgcaga ggtaggttat tcccgcttct
     2521 cggcccgcga ggtcgcgcgc agggtgggtt acgcggtggg caccctatac catgttttcg
     2581 gaacgctcga ccggctaatg ttcgagatca acacgatcac cttccgccaa tgggccgacc
     2641 tgctggagca tcggctcgcc gaagcggacg gccggcggct cgacgccctg gtcgaaggct
     2701 atttcgattt cgccatcgcc aaccgcaatc tgtggggagc gatctgggcc cacaac
//
LOCUS       DASWNU010000362         8550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_247487, whole genome shotgun sequence.
ACCESSION   DASWNU010000362 DASWNU010000000
VERSION     DASWNU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247487"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3034)
                     /locus_tag="VGB57_07255"
     CDS             complement(<1..3034)
                     /locus_tag="VGB57_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0131185.1"
                     /translation="MALTVKSAFTCTASTGALVLAGLIAAAPAAAQTPVPEQPAQAPA
                     PTGDAGTAEETLSDGGAATTEAQQEAVGEEEIVITGFRASLQNALSQKRRSNQIIDAI
                     TAEDIADFPDANLAESIQRLPGVSIDRDNGEGRSITVRGLGGDFQMVRLNGADAQAVS
                     GNGGSDSGANRSRGFDFNTFASELFGGITVTKTTSAANDEGSLGAIIDLVTGRPLAFK
                     TDRIALGAEAEYRENGSTWNPRLTALFSKRLGDNFGILGSIAYQDQEQQIDRYQRSIG
                     AFEYVYRNSLIAGTTPPVFGFARPGGTGATFGSDPAAYALLSPTTIFPALPSIGRQEL
                     SYDRLGATLTAQWRPMDRTEIVVDGVYSRFRQDNNTYAITPVGLNRNGTNARAQQTGT
                     AGLRAPGTTNGNADRVALYPNCVPSASTDCGASLYGGAVVPGFLNSQNPNNLNPFDYY
                     NNPRSVGFVPTANQTGFYNELIGRPNTKVRQASVNAAGQAEYLVLDDVDWRSSADSQM
                     GQTSFKQGTINLTHEFSDNLRMDGTAGWSKSKFRGTGLFAEFNAIDRDGYTYDERGDA
                     IMPVFNPGFNVADPTQWSLVKGLSTIRYVNTLVDNEFKVGRLNFAWDLDESFTLRFGG
                     TYKQYDFDSDQGRRNQDIEAINPTLREAGLQITDLGQTVGFGQGLTVSQGTPTSFYAP
                     NLDSFRQYFQIDCNCINRWGDYRAVVDGRQRNSITEKDLSGFVQLDYKFDLFGRPLRG
                     DVGLRVARTRVTGRGNVGATDGVLGLPVTANNSYTDYLPSLNAVYEVAPDFLVRFAAS
                     KTMARPMLQNLTPGTTSFASGLNATTAPAVTVGNPYLSPFRSTNLDLSFERYFGRSGL
                     IGVAFFYKDLKSFPQQLAGEAPLDTVFEPEIYQQVVAAMTSPTLQAYTQAGGVWAIRQ
                     FQDAPGGTIKGLEVNVQSDFGFIGDMFRNFGITANYTYIDSKLTYLTSTVLNTTRTGT
                     SPTAQNTFAEGPFLNTSPHSFNATLYYENETWSARVSGA"
     gene            complement(3250..4617)
                     /locus_tag="VGB57_07260"
     CDS             complement(3250..4617)
                     /locus_tag="VGB57_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEY0131186.1"
                     /translation="MKTGSCVAAALFACLASPAWAQARVSFPERVCDVTRYGARGTRV
                     FLDTEAFQRAIDDCHAKGGGTVEVPRGEYLIGPIFLKSNIRLHLARWSELVGTSDEQP
                     YRMSKGLEAWESNSGWIALINIADSSNVAISGEGRIDGQGPAWWERWRAQARKAMKGG
                     STNRPRLVHAARSRNLLFEGIGLYNSPSFHLVVKDSEDVTVRRMRFVALAHSPNTDAI
                     DPVDTRNMLIEENFFDVGDDIVAIKSMKPNPARPDAAVENIVIRRNKGRSGRGICIGS
                     ETIGGVRGVLVEDNELNGAMYGIRIKTPRGRGGLVQDIVFRNNRMTDVETPMVFSSYY
                     EGAGYDETAVANRLASGGFVLGHQIYPPDTDPAQPYKPNATPWIRNVRVEGLTATGAE
                     RAGIVVGLPERAIEGLVFRNVRIEARRGLLVRNAAVDARGLRVVAGDRQPLVKERGAE
                     VRTRR"
     gene            complement(4687..5043)
                     /locus_tag="VGB57_07265"
     CDS             complement(4687..5043)
                     /locus_tag="VGB57_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131187.1"
                     /translation="MRWMDFLADGATYLVIAAALAAGVAASYGRELEAGRYPNGQWWI
                     RRLLITPLLVIAATAATELFGLSKSGAAFTAAMLSIGGYDVIKLIEARWLRRIGLIDG
                     EPRAPPVGRDPPPPTE"
     gene            complement(5040..5222)
                     /locus_tag="VGB57_07270"
     CDS             complement(5040..5222)
                     /locus_tag="VGB57_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131188.1"
                     /translation="MILDRIDDYLRSTKTSPSRLGRDAVGDPNFVMNLRDGRQPRPAT
                     LDRVLAFIEQAEERFR"
     gene            5306..5962
                     /locus_tag="VGB57_07275"
     CDS             5306..5962
                     /locus_tag="VGB57_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEY0131189.1"
                     /translation="MDANDPRIALERLLKERGEDFAGLSRLIGKNPAYIQQFIKRGTP
                     RKLDEDDRRTIARYLGVSEALLGGPQGSRSDDSAEGMIKVPRLDVGASAGHGAITDGE
                     VAVSHIAFDPKWLRQLCKGGTNALSFIRVQGDSMSPTLADGDDILVDGGDGADRLRDG
                     IYVLRRDDTLMVKRLAINPFAARATITSDNPAYPEWKDIELSTLAIIGRVVWAGRRLS
                     "
     gene            6147..6950
                     /locus_tag="VGB57_07280"
     CDS             6147..6950
                     /locus_tag="VGB57_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0131190.1"
                     /translation="MIYGYDPAPDRHARLLKAHRMAMDMLGAAEKAGLFRAGVTELEL
                     SRELQALGQAMFGKFKHWHKRIVRAGPNTLSTFGDSPPDLRVEADDMVFVDLGPMFES
                     WEADIGRTFVIGDDPGRMRLRDAIEATWYAGRDHFKKNRDSVTGADMYELALEAARSN
                     GYSFANWHAGHLIGNFPHEIVQGELSENYLHAGNRLPLAGPDRDGNPRSWIYEVHFID
                     PERGYGGFFEQWLELDDPAPASRRAELVAASGVEPPCAREDCAPETSSG"
     gene            7064..7561
                     /locus_tag="VGB57_07285"
     CDS             7064..7561
                     /locus_tag="VGB57_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0131191.1"
                     /translation="MAGQSRPGTARNEDDQVVVRLKALSDEAAHLASALASRPPEGKA
                     EGAPRRSGHPSPEEVLKLLIDVRRIRFRHFAESSFADPGWDILLDLMSARLSKRPVPV
                     SSACVAAGVPATSALRWVDQLAKSGLVQRLPDPSDRRRVLLELTEEGCRRMEVYLRAV
                     GRLLR"
     gene            7753..>8550
                     /locus_tag="VGB57_07290"
     CDS             7753..>8550
                     /locus_tag="VGB57_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0131192.1"
                     /translation="MDRTSEQRPPGEEQSRPADPALACFILLARFLGVPADPRQIGHD
                     RGKGEDPYPREDLSRIARKLALIGRIRRCEADQVHKLPLPAIAELGDGDAAILLKVED
                     ESDSPRVLVQLGDSDRPEVWSMAEFRERFGGRLLLMTARERMAGAARPFDISWFIPAL
                     VKYRKPLRDVLIASFFLQLMGLVSPIFFQLIIDKVLVHQSMTTLDVLAIGLAVILIWE
                     TGLSGLRNWLFAHSTNRVDAELSARMFRHLLNLPLSYFEARRVGDSIA"
BASE COUNT         1381 a         2713 c         2949 g         1507 t
ORIGIN      
        1 aggcccccga aacgcgcgcc gaccaggttt cgttctcata atagagggtc gcgttgaacg
       61 agtgcggcga ggtgttgagg aacggcccct cggcgaaggt gttctgcgcc gtcgggctgg
      121 tgcccgtccg ggtcgtgttg agaacggtgc tggtgaggta ggtcagcttc gaatcgatgt
      181 aggtgtaatt ggccgtaatc ccgaagttcc ggaacatgtc cccgatgaat ccgaagtccg
      241 actggacatt gacctcgagg cccttgatcg tcccgccggg agcgtcctgg aactgccgga
      301 tcgcccagac tccgccggcc tgggtatagg cctgcagggt cgggctggtc atcgcggcga
      361 cgacctgctg gtagatctcc ggctcgaaca cggtgtcgag cggcgcttcg ccggcgagct
      421 gctgcgggaa ggacttcaga tccttgtaga agaaggccac cccgataagg ccgctccgtc
      481 cgaaatagcg ctcgaagctg aggtcgagat tggtcgaccg gaacggactc agatagggat
      541 tgccgacggt caccgccggc gccgtggtcg cgttgaggcc gctcgcgaag ctcgtcgttc
      601 ccggcgtcag attctggagc atcggccggg ccatcgtctt cgacgcggcg aagcgcacca
      661 ggaagtccgg agcgacctca tagaccgcgt tgagcgacgg cagatagtcc gtgtagctgt
      721 tgttcgccgt caccggcagg ccgagcactc cgtcggtggc gccgacattg ccgcggccgg
      781 tgacccgggt gcgcgccacc cggagcccga cgtcgccgcg cagcgggcgg ccgaacaggt
      841 cgaacttata gtcgagctgg acgaagccgg agagatcctt ctcggtgatc gagttgcgct
      901 gccgcccgtc gaccactgcg cggtaatcgc cccagcggtt gatgcagttg cagtcgatct
      961 ggaaatattg gcggaaggag tcgaggttcg gggcgtagaa gctcgtcggg gtgccttgcg
     1021 agacggtcag gccctggccg aagccaaccg tctggccgag gtccgtgatc tggagcccgg
     1081 cttccctcag agtcgggttg atcgcctcga tgtcctggtt gcggcggccc tggtcgctgt
     1141 cgaaatcata ttgcttgtag gtgccgccga agcggagggt gaagctctcg tccaggtccc
     1201 aggcgaagtt gaggcggccg accttgaact cgttgtcgac cagggtgttg acgtagcgga
     1261 tcgtggacag gcccttgacc aggctccact gggtcggatc ggcgacgttg aagccggggt
     1321 tgaacaccgg catgatcgcg tcgccgcgct cgtcataggt gtagccgtcg cggtcgatgg
     1381 cgttgaactc ggcgaacagt ccggtgcccc ggaacttcga cttcgaccag ccggcggtcc
     1441 cgtccatccg taggttgtcg ctgaattcgt gggtgaggtt tatcgtgccc tgcttgaagc
     1501 tggtctggcc catctgggag tcggccgagc tgcgccagtc gacgtcgtcc agcaccagat
     1561 attcggcctg gccggcggcg ttgacgctgg cctggcggac cttggtgttc ggccggccga
     1621 tcagctcgtt gtagaatccg gtctggttgg cggtggggac gaagcccacc gagcgcgggt
     1681 tgttgtaata atcgaacgga ttgaggttgt tcggattctg gctgttgagg aagcccggaa
     1741 cgaccgctcc gccgtacagg gaggcgccgc aatcggtcga ggcgctcgga acgcagttcg
     1801 ggtagagcgc cacccggtcg gcattgccgt tggtcgtgcc cggcgcgcgc aggccggcgg
     1861 taccggtctg ctgcgcccgg gcgttggtgc cgttgcggtt gaggccgacg ggcgtgatcg
     1921 cgtaggtatt gttgtcctgc cggaagcgcg aatagactcc gtcgacgacg atctcggtcc
     1981 ggtccatcgg gcgccactgc gccgtgagcg tggcgccgag acgatcgtag gatagctcct
     2041 ggcggccgat cgagggcaag gccgggaaga tggtggtcgg gctgagcagg gcgtaagcgg
     2101 ccggatcgga gccgaaggtc gcgcccgttc cgccgggccg ggcgaagccg aagacgggcg
     2161 gggtcgtgcc ggcgatcagc gagttgcggt agacatattc gaacgctccg atgctgcgct
     2221 ggtagcggtc gatctgctgc tcctggtcct gataggcgat cgagcccaag atcccgaaat
     2281 tgtcgccgag acgcttcgag aagagcgccg tgaggcgcgg attccaggtg cttccattct
     2341 cgcgatattc ggcttccgcg ccgagcgcga tccggtcggt cttgaaggcg agggggcggc
     2401 cggtgacgag atcgatgatc gctccgagcg agccttcgtc gttggccgcc gaagtggtct
     2461 tggtgaccgt gattccgccg aacagttcgg aggcgaaggt gttgaagtcg aagccccgcg
     2521 agcggttcgc gcccgaatcg gagccgccgt tgccggacac cgcctgggcg tcggcgccgt
     2581 tcagccgcac catctggaag tcgccgccga gcccgcgcac cgtgatcgag cggccttcgc
     2641 cattgtcgcg gtcgatcgat acgccgggaa ggcgctggat cgattcggcg aggttcgcgt
     2701 ccgggaagtc ggcgatgtcc tccgccgtga tcgcgtcgat gatctggttc gagcggcgct
     2761 tctggctgag cgcgttctgg agcgaggctc ggaagccggt gatgacgatc tcttcctcgc
     2821 cgaccgcttc ctgctgggcc tcggtggtcg cggcgccgcc gtcggacagg gtctcctcgg
     2881 ccgtgccggc atcgccggtg ggagcgggag cctgcgccgg ctgctccggc accggggtct
     2941 gagcagcggc cggggcggcc gcgatcagcc ccgcgaggac cagcgcgccc gtggacgccg
     3001 tgcacgtgaa tgcggatttg acagtgagag ccaatcgaac ctccgtctcc aggagtcctc
     3061 cggtgttccg gctggacaga atagatgtgc caccggtgtc atccgctagg acggaagccg
     3121 atcatcggag cttcgcgtca ttggcgacgc gcctgccgtg ttcgattgcc cccaccgcaa
     3181 aaatgtcacc ggtgtcaaag ccttataacc tcggttctgc aggattcgca aggggctggt
     3241 cgacgggcgt cagcgccgcg tccgcacctc cgctccacgc tctttcacca gcggctgccg
     3301 atcgcccgca acgacccgca gtccccgcgc gtcgacggcc gcgttgcgga ccagcagccc
     3361 gcggcgggcc tcgattcgca cgttgcggaa caccaggccc tcgatcgccc gctcgggcag
     3421 gccgacgacg atgcccgcgc gctcggcccc ggtggcggtc aggccctcga cccgcacgtt
     3481 gcggatccag ggcgtggcat tcggcttgta gggctgcgcc ggatccgtgt cgggcgggta
     3541 gatctggtgg ccgaggacga agccccccga agcgaggcgg ttcgcgaccg ccgtctcgtc
     3601 gtagccggcg ccttcataat agctggaaaa gaccatcgga gtctcgacgt cggtcatccg
     3661 gttgttgcgg aagacgatat cctggacgag gccgccgcgg ccgcgcgggg tcttgatccg
     3721 gatgccgtac atcgccccat tgagctcgtt atcctcgacc agcacgccgc ggacgccgcc
     3781 gatcgtctcg ctgccgatgc agatgccgcg tcccgaacgg cccttgttgc ggcggatgac
     3841 gatgttctcg accgccgcgt ccggccgcgc cggattgggc ttcatcgatt tgatcgcgac
     3901 gatgtcgtcg ccgacgtcga agaaattctc ctcgatcagc atgttgcgag tgtcgaccgg
     3961 gtcgatcgcg tcggtgttgg gggaatgggc gagggcgacg aagcgcatcc gccgcaccgt
     4021 cacgtcctcg ctgtccttga cgacgagatg gaagctcggc gaattgtaga ggccgattcc
     4081 ctcgaacagc aggttccggc tgcgggcggc gtggacgagg cgcgggcggt tggtcgagcc
     4141 gcccttcatc gccttgcgcg cctgggcccg ccagcgctcc caccaggccg ggccctggcc
     4201 gtcgatccgg ccttcgcccg aaatggcgac gttgctcgaa tcggcgatgt tgatgagggc
     4261 gatccagccg gaattgctct cccaggcttc gaggcccttg ctcatccggt agggctgctc
     4321 gtcgcttgtc ccgaccagct cggaccagcg cgcgaggtgc agccggatgt tggatttgag
     4381 gaagatgggg ccgatcagat attcgccccg cggcacctcg accgttccgc cgcctttggc
     4441 gtgacaatcg tcgatcgcgc gctggaaggc ctcggtgtcg aggaagacgc gagtgccgcg
     4501 ggcgccgtag cgggtcacgt cgcagacccg ctcgggaaaa gagacccggg cctgcgccca
     4561 ggccggcgac gcgaggcagg cgaacaaggc ggcggcgacg catgatcctg ttttcatcct
     4621 gcctcccttg gcacagcggc gccgcgctag aagcgaaggg ccgtgaaagg caagcgggaa
     4681 gcggtgctat tcggtgggcg gtggagggtc gcgcccgacc gggggagcgc gcggctctcc
     4741 gtcgatcagg ccgatccggc gcagccagcg cgcttcgatg agcttgatga cgtcgtagcc
     4801 gccgatcgaa agcatcgcgg cggtgaaggc ggcgccgctt ttgctgaggc cgaaaagctc
     4861 ggtcgccgcg gtcgccgcga tgaccaggag gggcgtgatg agcagccgcc ggatccacca
     4921 ttggccgttc ggatagcggc ccgcctcgag ctcgcggccg taggatgccg ctactccggc
     4981 cgccagcgcc gcggcgatga cgaggtaggt cgcgccgtcg gccaggaaat ccatccatct
     5041 catcggaatc gttcctccgc ctgttcgatg aaggcgagga ctcggtcgag cgtggccggg
     5101 cgcggctgcc gtccgtcgcg caggttcatc acgaaattgg gatcgcccac cgcgtccctg
     5161 ccgagccgcg acggagaggt tttggtgctg cgaagataat cgtcaatgcg gtcgaggatc
     5221 atgaaaagct ccgaatcgtc ttattcctat cctcgcatga agattcctac ttgtctagga
     5281 gaattcctgc cactaaagag gggagatgga cgcgaacgat ccacgaatcg cgcttgagcg
     5341 cctcctcaag gaacgggggg aggattttgc cggcctgtcg cggctgatcg gcaagaatcc
     5401 ggcctacatc cagcagttca taaagcgcgg tacgccgcgc aagctggacg aggacgatcg
     5461 ccggaccatc gcccgctatc tcggggtcag cgaggcgctg ctgggtggcc cccaaggctc
     5521 ccgcagcgac gattccgcgg aggggatgat caaggtgccc cgcctggatg tcggcgcttc
     5581 ggccggccat ggcgcgatca ccgacggcga agtcgccgtc tcgcatattg ccttcgatcc
     5641 caagtggttg cgccagctct gcaagggcgg caccaacgct ctgtccttca tccgggtgca
     5701 gggcgattcg atgagcccca ccctggccga cggcgacgac atcctggtcg acggagggga
     5761 cggggccgac cggctccgcg atggaatcta cgttctgcgc cgggacgaca cgctgatggt
     5821 gaagcgcctt gcgatcaacc ccttcgcggc gcgggcgacg atcaccagcg acaatcccgc
     5881 ctatccggaa tggaaggata tcgagctgtc caccctcgcc atcatcggac gggtggtgtg
     5941 ggcgggccgc aggctcagct agcccctgcc ggcacgacgg cgcagcaggg ccggcacgaa
     6001 gaataggaaa cagcctgaga ccttggcggg gacggcggtt cgcgccgcga cggcgcctgt
     6061 cctcaaggat gctctcgcct tcccgggccc gatttcatac accccgacgg cggctgaccc
     6121 gctccaggct cggacatgaa ggcgatatga tctacggcta cgaccctgcc cccgaccggc
     6181 acgccaggct cctgaaggcc caccggatgg cgatggatat gcttggcgcg gcggagaaag
     6241 cgggcctgtt cagggcgggg gtgacggagc tggagctgag ccgggagctt caagcgctcg
     6301 gccaggcgat gttcggaaag ttcaagcact ggcacaaaag gatcgtcagg gccggaccga
     6361 acaccctttc gaccttcggc gacagcccgc ccgaccttcg ggtcgaggcc gacgacatgg
     6421 tgttcgtaga tctggggccg atgttcgaat cctgggaggc ggacatcggc cggacctttg
     6481 tgatcggcga cgatcccggc cggatgcggc tgcgggatgc gatcgaggcg acctggtatg
     6541 ccggccgaga ccatttcaag aagaaccgcg attccgtcac cggcgccgac atgtacgagc
     6601 tggccctcga ggcggctcgc tccaatggct acagcttcgc gaactggcat gcgggtcacc
     6661 tcatcggcaa cttcccgcac gagatcgtcc agggcgagct cagcgagaac tacctccatg
     6721 ccggcaaccg cctgccactg gccggtcccg accgcgacgg caatccgcgg agctggatct
     6781 acgaagtcca tttcatcgac ccggagcggg gctatggcgg cttcttcgag cagtggctcg
     6841 agctcgacga tcccgcgccc gcgtcccgtc gggccgagct tgttgcggcg tccggcgtcg
     6901 agccgccctg cgcaagagaa gattgcgccc ctgaaacaag ttcagggtga cgccccagcc
     6961 atattgtgcc actgtcccga acactggtat tgaccgtcgc cgcgcgggag tgggacgatt
     7021 tcgatcggtt ccgcgcgggg gcctcacggg gctgaggatc gacgtggcag ggcaatcgag
     7081 accggggacc gcccggaacg aggacgatca ggtcgtcgtg cgcctgaagg cgctgagcga
     7141 cgaagccgcg catctcgctt cggcgctcgc ctcgcggccg ccggagggca aggcggaagg
     7201 agcgccgcgg cgctccggac atccttctcc ggaagaggtg ctcaagctgc tgatcgatgt
     7261 gaggcggatc cgcttccgcc actttgccga atcctcgttt gccgaccccg gctgggatat
     7321 cctgctcgac ctgatgtcgg cgcgtctttc gaaacggccg gttccggtct cgagcgcctg
     7381 cgtggcggcc ggagtcccgg cgacgagcgc gctccgctgg gtcgaccaac tcgccaagtc
     7441 gggactggtc cagcgtttgc cggatccgag cgaccggcgg cgcgtcctgc tcgagctgac
     7501 ggaggaagga tgccgccgca tggaggtcta tttgcgggcc gtcggccggc tcctgcgctg
     7561 agaatatccg ccgatctggt gcgaatatct cccaatccga agtgctgtta gaaaaatggc
     7621 ccggcttttg gatcaattgc cgctggcttc atcttttttc tatatataac agggacttaa
     7681 tcctagcgcg ccgtctctga ttcgccccgc catcgtgggc gtccgaggcg cgacttgtaa
     7741 cagcggggaa acatggatcg caccagtgaa cagcgccctc cgggcgagga gcagtcgcgt
     7801 cccgccgatc cggcgctcgc ctgcttcatc cttctcgccc gctttctcgg ggttccggcc
     7861 gatccgaggc agatcggcca cgaccgcggc aagggcgagg atccctatcc gcgcgaggac
     7921 ctgtcgcgca tcgccaggaa gctggcgctg atcggacgga tccgccgctg cgaagccgat
     7981 caggtccaca agctgccgct gcccgcgatc gccgagctcg gcgacgggga cgcggccatc
     8041 ctcctcaagg tcgaggacga aagcgactcg ccgcgggtcc tggtccagct cggcgacagc
     8101 gatcgcccgg aagtgtggag catggcggag ttccgcgagc gcttcggcgg ccggctgctg
     8161 ctgatgacgg cgcgcgagcg catggccgga gcggcccgcc cgttcgacat cagctggttc
     8221 atccccgccc tggtcaaata tcgcaagccg ctcagggacg tgctgatcgc ctccttcttc
     8281 ctccagctga tggggctggt ctccccgatc ttcttccagc tcatcatcga caaggtgctc
     8341 gtccatcaat cgatgacgac gctcgacgtg ctcgccatcg gccttgccgt catattgatc
     8401 tgggaaaccg gcctttcggg ccttcggaac tggcttttcg cgcacagcac gaaccgggtg
     8461 gacgccgagc tcagcgcccg gatgttccgc catctgctca acctgccgct ctcctatttc
     8521 gaggcgaggc gggtcgggga cagcatcgcc
//
LOCUS       DASWNU010000363         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_273204, whole genome shotgun sequence.
ACCESSION   DASWNU010000363 DASWNU010000000
VERSION     DASWNU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273204"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1852
                     /locus_tag="VGB57_07295"
     CDS             38..1852
                     /locus_tag="VGB57_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY0131193.1"
                     /translation="MDRRTFIASSGAAALAATLPGAGLAQPSAGSGDAALNALFERIF
                     QQNVQRSPELATSLGLDKDANAGLKGRLSPRTLAERARVLAQTKEALAELAAVDRRTL
                     SPAAKLNLEVVTYSLTNQTTAPRRFGLDSAIRPYRIFQQGGAYFSVPDFLNTAHTIND
                     KDDSEAYLARLEAFAVALDQDSAMQRIEAARGYVAPAWSLDLTLGQMAKLRGQKADES
                     SIVQSLAKRAKARDIDGRWERRASRIVEERVYPALDRQMALIRELKPMSQPGDGIWRV
                     PHGDEIYAVALEQATTTRFSPAEVHRMGLQQVAEISAELDRILKSQGFTRGSVGERLA
                     ALNVTPSQVYPNTDDGRAELIAGLNESAKAMDARLPAAFATLPGAPLEIRRVPPEIQD
                     GASNGYYNRASLDGSRPAIYFINLKDTGDWPKYSLPALTYHEGNPGHHLQISLAQESK
                     DIPTLRKVGGFSAYSEGWALYAEQLADELGAYATPLERAGYLQSFLFRAARLVVDTGL
                     HSQKWTREKATDYLVATTGFARPRSQREVERYCTQAGQACSYKVGHMAWSRARAEAKR
                     ALGRRFDLKQFHEVLKDGAMPLTILERRIRERTAAARG"
     gene            complement(2007..2225)
                     /locus_tag="VGB57_07300"
     CDS             complement(2007..2225)
                     /locus_tag="VGB57_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131194.1"
                     /translation="MHPVRAFFTSLSMSLLVLTLSYQASAIPAALTPPMPMPRSDAWM
                     DHTSAVKKAKKGEDKDDMSELVPLLSAS"
BASE COUNT          421 a          820 c          848 g          361 t
ORIGIN      
        1 ccgcctaaga ccttggcagt caagcaggag cccaaccttg gatcgccgca ccttcatcgc
       61 ctcgtcgggc gccgccgccc tcgccgcaac ccttcccggt gccggcctcg cccagccgag
      121 cgccggctcc ggcgacgcgg cgctcaacgc cctgttcgag cgcatcttcc agcagaatgt
      181 gcagcgctcg cccgagctcg cgacctcgct cggcctcgac aaggatgcca atgcggggct
      241 gaaggggcgg ctcagcccgc ggacgctggc ggagcgggcc agggttcttg cgcagaccaa
      301 ggaagcgctg gccgagctgg cggcggtcga ccgcaggact ctctcgccgg cggcgaagct
      361 caacctcgag gtcgtcacct actcgctgac caaccagacg accgctccgc ggcgcttcgg
      421 cctcgacagc gcgatccggc cctaccggat cttccagcag ggcggcgcct atttctcggt
      481 gcccgatttc ctcaacaccg cgcacacgat caacgacaag gacgattccg aggcctatct
      541 ggcccggctc gaggcgttcg cggtggcgct cgaccaggac agcgcgatgc agcggatcga
      601 ggcggcgcgg ggctatgtcg ctccggcctg gtcgctcgat cttacgctcg gccagatggc
      661 caagctgcgc gggcagaagg ccgacgaaag cagcatcgtc cagtcgctgg cgaagcgcgc
      721 caaggccagg gatatcgacg gacgctggga gcggcgggcg tcccggatcg tcgaggagcg
      781 ggtctatccg gcgctcgacc gtcagatggc gctgatccgg gagctgaagc cgatgagcca
      841 gccgggcgac ggaatctggc gggtgccgca tggcgacgag atttacgcgg tcgcgctcga
      901 gcaggcgacg acgacccgct tcagccccgc cgaggtccac cggatggggc ttcagcaggt
      961 ggccgagatc agcgccgagc tcgacaggat cctgaagagc cagggcttca cccgcggaag
     1021 cgtcggcgag cggctggcgg cgctcaacgt cacgccctcc caggtctatc cgaacaccga
     1081 cgacggccgg gccgaactca tcgccggctt gaacgaaagc gccaaagcga tggacgcccg
     1141 gcttccggcg gccttcgcca ccctgccggg ggctccgctc gaaatccggc gggtgccgcc
     1201 cgagatccag gacggcgctt cgaacggcta ttacaaccgc gcctcgctgg acggttcgag
     1261 gccggcgatc tacttcatca acctcaagga taccggcgac tggccgaaat actcgcttcc
     1321 ggcgctcact tatcacgagg gcaatccggg ccatcacctc cagatcagcc tcgcccagga
     1381 atcgaaggac attccgacgc tgaggaaagt gggcggcttc agcgcctata gcgagggctg
     1441 ggcgctttat gccgagcagc tcgccgacga gctcggcgcc tatgcgactc cgctcgagcg
     1501 ggcgggctat ctccagtcct tcctgttccg ggcggcccgg ctggtcgtcg acaccggcct
     1561 ccattcgcag aaatggacgc gggagaaagc gaccgactat ctcgtcgcca ccaccggctt
     1621 cgcccggccg cgctcccagc gcgaggtcga gcgctactgc acgcaggcgg ggcaggcgtg
     1681 cagctacaag gtcggccaca tggcctggag ccgggcccgg gcggaggcga agcgtgcgct
     1741 cgggcgccgg ttcgacctca agcagttcca cgaggtcctg aaggacggag cgatgccgct
     1801 caccatcctc gagcggcgga taagggagcg gacggcggcg gcgagggggt aggcgcctca
     1861 gaatcctccc cggcctttgc cggggagggg gaccatgcga agcatggtgg aggggttctt
     1921 ttgggggctc gaagaacccc tccaccgaac ttcgttcggt ccccctcccc cggaaatccg
     1981 ggggaggaat taggttggcc cggctcttac gaagcgctga gcagcgggac cagctcggac
     2041 atgtcgtcct tgtcctcgcc cttcttcgcc ttcttgaccg ccgaggtgtg gtccatccag
     2101 gcgtcgctcc tgggcatggg catcggcggg gtgagggcgg ccgggatggc gctcgcctga
     2161 tagctcaagg tcagcaccag caggctcatc gaaagcgagg tgaagaaggc gcggaccgga
     2221 tgcatgatga aagtcgtccc ttggaagtct caggtcggat gcggctccgg ccagcgagcg
     2281 gagggggcaa aacgccgccg accggagccg catcgccgtt ctggcggcgg cccgtgtccg
     2341 ggcgatgccg cggaaggggc aagttggtgt caattgtgtc agggggcagg gaggaagtac
     2401 gtcatcccgg cgaaggccgg gatccatgaa cacaggggca ggcgattacc
//
LOCUS       DASWNU010000364         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_14493, whole genome shotgun sequence.
ACCESSION   DASWNU010000364 DASWNU010000000
VERSION     DASWNU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14493"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VGB57_07305"
     CDS             <1..1010
                     /locus_tag="VGB57_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0131195.1"
                     /translation="PSALDNRPLKFEEWEAMRPQTVHVSATPGPWEMTKAGGVFAEQV
                     IRPTGLIDPPVEVRPVTGTTANQVDDVIAEVKDTAAAGYRSLVTVLTKKMAEDLTEYM
                     HEQGVRVRYLHSDVDTMERIEIIRDLRLGGFDCLIGINLLREGLDIPECGLVAILDAD
                     KEGFLRSETSLIQTIGRAARNVDGKVILYADRVTGSMERAMAETNRRRAKQEQYNLEH
                     GITPESVKSNIKEILASPYEKDRVTIPVGVAEDSKPFLGSNFQATVRDLETRMRQAAA
                     DLEFETAARLRDELKRLKLLDLEFANDMVGSGGKVEREEQFAAGTPQAVTQAKRTGRR
                     R"
     gene            complement(1161..1601)
                     /locus_tag="VGB57_07310"
     CDS             complement(1161..1601)
                     /locus_tag="VGB57_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131196.1"
                     /translation="MRRSVFGTMAALGLGVGLGLAATSVAAQSTTDGAVDGRIEWDIR
                     VLGCDGAKWLARWEGDRIRTWRDTDASPELGRVAAEMHYRTWGGECWWAKWDPAASAE
                     GLFEHHNVRTQASHLDSILNYVDWDGREWTVRRKGENWVMTPRG"
BASE COUNT          342 a          680 c          652 g          265 t
ORIGIN      
        1 tgccctcggc cctcgacaac cgcccgctca agttcgagga gtgggaggcg atgcggcccc
       61 agacggtcca cgtctcggcc acgccgggcc cgtgggagat gaccaaggcc ggcggcgtct
      121 tcgccgagca ggtcatccgc cccaccggcc tgatcgaccc gccggtcgag gtgcgcccgg
      181 tcacgggaac caccgccaac caggtcgacg acgtcatcgc cgaggtgaag gatacagccg
      241 ccgccggcta ccgctccctg gtcaccgtcc tgaccaagaa gatggccgag gacctgaccg
      301 aatacatgca cgagcagggg gtgcgggtgc gctacctcca ctccgacgtc gacaccatgg
      361 agcggatcga gatcatccgc gacctgcggc tcggcggctt cgactgcctg atcggcatca
      421 acctgctccg ggaaggcctc gacatccccg agtgcggcct ggtcgccatc ctcgacgccg
      481 acaaggaggg cttcctgcgc tcggagacga gcctgatcca gaccatcggc cgcgccgccc
      541 gcaacgtcga cggcaaggtc atcctctacg ccgaccgggt caccggctcg atggagcggg
      601 ccatggccga gacaaaccgg cgccgcgcca agcaggaaca gtacaacctc gagcacggca
      661 tcacgcccga gagcgtcaag tcgaacatca aggagatcct tgccagcccc tacgagaagg
      721 accgcgtcac catccccgtg ggcgtggccg aggactccaa gccgttcctg ggctccaact
      781 tccaggcgac ggtcagggac ctggagaccc gcatgcgcca ggccgccgcc gacctggagt
      841 tcgaaaccgc cgcccgcctg agggacgagc tcaagcggct gaagctgctg gacctggagt
      901 tcgccaacga catggtgggg tcaggtggga aggtggagcg ggaggagcag ttcgcggcgg
      961 ggacgccgca ggcggtgacc caggcgaagc ggacggggcg gcggcggtag ggaccttcgc
     1021 ccaaaaggcc ctcttgatat ccgctcatcc cggcggaagc cggggcccag tctaaggcca
     1081 gaagcgcggt gctctccgca aagaactggg tcccggcttc cgccgggatg agcggggtaa
     1141 aaaatggcgc cgcgcaccgt tcacccccgc ggggtcatca cccagttctc gcccttgcgc
     1201 cgcacggtcc actcgcgccc gtcccagtcg acgtagttga ggatcgagtc caggtggctc
     1261 gcctgggtgc gcacgttgtg gtgctcgaac aggccctcgg ccgacgcggc cgggtcccat
     1321 ttggcccacc agcactcccc gccccaggtg cgatagtgca tctcggcggc gacccgcccg
     1381 agctcgggcg aggcgtcggt gtcgcgccag gtgcggatcc ggtcgccctc ccaccgcgcc
     1441 agccacttgg cgccgtcgca gccgagcacg cggatgtccc actcgatccg cccgtcgacc
     1501 gccccatcgg tggtggattg agccgccacg gacgtcgccg ccagccccag ccccacgcca
     1561 agtcccaagg ccgccatcgt cccgaacacg gagcgcctca cgcttcggtg gtcgtgctca
     1621 tcgccgatcc tcctgcagca agggcggagg taagcacgga tggggcgggg tggctgacga
     1681 tgcggagccg ccttgaactt cgagaccgcc gcccgcctgc gcgacgagct gaagcggctg
     1741 ttggacctgg agttcgccaa cgacatggtc ggcggcgggg gaaggtggag cgcgaggaaa
     1801 tgtacccggc agggactccg caggcggaga caatccaagc ggatggggcg gcggttaggt
     1861 gcccgcccta cggttcttgt gatatcgctc gctgcgaaac caacggagca tctaactcgg
     1921 cgtcgcccaa ggctttaaa
//
LOCUS       DASWNU010000365         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_507085, whole genome shotgun sequence.
ACCESSION   DASWNU010000365 DASWNU010000000
VERSION     DASWNU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507085"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VGB57_07315"
     CDS             complement(<1..584)
                     /locus_tag="VGB57_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEY0131197.1"
                     /translation="MYQYDEIKPGAGGGAAALAFGPQLRPRSFGSRAVPPPRSFDLVV
                     DLGSRIGSGTWFRGVFTCTALCGIALWLAPGFDPIPAASAKPLSDAQFEQARSLAISP
                     LALGADSGRRMAPTDAVEPLLDRPERPTVEMVATLGEGDGFVRALERAGVAPREADEV
                     AKLVAAAVPIDQIKAGTTMNIVLGKRPNRTVARPL"
     gene            853..1158
                     /locus_tag="VGB57_07320"
     CDS             853..1158
                     /locus_tag="VGB57_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0131198.1"
                     /translation="MTRKSTDKPGYVYIIASRRNGTIYIGVTSDLPSRIYRHKIGAVR
                     GFTRRYGCKILVWYQAFDQLDEARRRELQMKEWQRAWKLRVIEEMNPDWADLYESLF"
     gene            1277..>2313
                     /locus_tag="VGB57_07325"
     CDS             1277..>2313
                     /locus_tag="VGB57_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0131199.1"
                     /translation="MSDFARAPLTAILGPTNTGKTHLAVERMAAHSSGMIGFPLRLLA
                     REVYDRVVAMKGANRVALITGEEKIVPKDARWFLCTAESMPMERDFAFVALDEAQLGA
                     DPERGHVFTDRMLHARGREETMILGSETLKPMVRALLPKAEIIGRPRFSTLSYAGPSK
                     LSRLPPRSAIVAFSAEQVYAVAEMLRRMRGGAAVVMGALSPRTRNAQVAMYQAGEVDY
                     LVATDAIGMGLNMDVAHVAFAGLSKFDGRRQRRLTPAEMAQIAGRAGRHQKDGSFGIL
                     TIESEGNAEFRPEEIEAIEEHRFAPLEFLYWRESDLDFRSVEDLIRSLERWPVRREVR
                     AAPEAVDLSVL"
BASE COUNT          394 a          752 c          823 g          344 t
ORIGIN      
        1 agcggccggg cgacggttcg gttggggcgt ttgccgagca cgatgttcat cgtcgtgccg
       61 gccttgatct ggtcgatcgg cacggcggcg gcgacgagct tcgcaacctc gtccgcttcc
      121 ctgggggcga ccccggcgcg ctcgagcgcc cgcacgaagc cgtcgccttc gccgagagtc
      181 gcgaccatct cgacggtcgg ccgctcgggc cggtcgagca acggctcgac cgcgtcggtc
      241 ggcgccatcc ggcggccgct atcggcgccc agggcgagcg gcgagatggc gaggcttcgg
      301 gcctgctcga actgggcgtc ggaaagcggc ttggccgagg ctgccgggat cggatcgaag
      361 cccggcgcga gccacagggc gattccgcac aaggcggtgc aggtgaagac gccccggaac
      421 caggtgcccg aaccgatgcg cgagccgagg tcgacgacga gatcgaaact gcgcggcggc
      481 ggcacggcgc ggcttccgaa gctgcgcgga cggagctgcg gaccgaaggc gagcgcggcg
      541 gctccgccgc cggcaccggg cttgatctca tcatactggt acaaggcgga aaaacccccg
      601 cgacccgtga aacacccccc ggccgcggat cgaggccagg tggcgaggac tgtggcgaaa
      661 cggggttaaa gtccagtaaa ccgggtgaaa cccggcgcga ggttgcggcg aagcgtgccg
      721 cgggagccgg tgaagcgtga gcgcctgcgg gaactacggc ttcccggcga aggccgggac
      781 ccagagcggc ggcagagctg ggccccggcc ttcgcgggga gcaatgtgcg cttgtgcaga
      841 atcacgcaag atattacacg aaaatcgacg gataaaccgg gctatgtcta cattatcgcc
      901 agccggcgca acgggacgat ctacatcggc gtgaccagcg acctgccgtc gcggatctac
      961 cggcacaaga tcggtgcggt gcggggcttc acccgcagat acggctgcaa aatcctggtc
     1021 tggtatcagg cattcgacca actcgacgag gcgcggcgcc gcgaattgca gatgaaggag
     1081 tggcagcgcg cgtggaagct gcgcgtcatt gaggagatga acccggattg ggcagatttg
     1141 tacgagtccc tcttctagtt tcccggcgaa ggccgggatc cagctccgac ccagaactgg
     1201 gccccggcct tcgccgggga gcaaaggaaa atccaagtca ttgcgtccga ggcatgaaat
     1261 gacgatctag gctcccatga gcgatttcgc gcgggcgccg ctgacggcga tccttggacc
     1321 caccaacacc ggcaagaccc acctcgccgt cgagcggatg gcggcgcatt cgagcgggat
     1381 gatcggcttc ccgcttcggc ttctggcgcg cgaggtctac gaccgggtgg tggcgatgaa
     1441 gggcgcgaac cgggtcgcgc tcatcaccgg cgaggagaag atcgtcccaa aggacgcgcg
     1501 ctggttcctg tgcacggcgg aatcgatgcc gatggagcgc gacttcgcct tcgtcgccct
     1561 ggacgaggcc cagctcggcg ccgaccccga gcgcggccac gtcttcaccg accggatgct
     1621 tcacgcccgc ggccgcgagg agacgatgat cctcggctcg gaaaccttga agccgatggt
     1681 ccgagcgctt ctgccaaagg cggagatcat cggccggccg cgtttctcga ccctttccta
     1741 tgccgggccc tcgaagctct cgcggctgcc gccgcgctcg gcgatcgtcg ccttctcggc
     1801 cgagcaggtc tacgcggtcg ccgagatgct ccggcggatg cgcggcgggg cggcggtggt
     1861 gatgggggcg ctttcccctc gcacccgcaa cgcccaggta gcgatgtatc aggcgggcga
     1921 ggtcgattat ctcgtcgcca ccgacgcgat cggcatgggc ctcaacatgg acgtcgccca
     1981 tgtcgccttt gcgggccttt ccaagttcga cggcaggcgc cagcgccggc tgacgccggc
     2041 cgagatggcg cagatcgccg gccgcgccgg gcggcaccag aaggacggca gcttcggcat
     2101 cctgaccatc gagagcgaag gcaatgccga gtttcgcccg gaggagatcg aggcgatcga
     2161 ggagcatcgc ttcgcgccgc tggaattcct ctactggcgc gagagcgacc tggactttcg
     2221 ctcggtcgag gacctgatcc gctcgctcga gcggtggccg gtccgccgcg aggttcgcgc
     2281 cgccccggag gcggtggacc tctccgtgct gag
//
LOCUS       DASWNU010000366         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_14535, whole genome shotgun sequence.
ACCESSION   DASWNU010000366 DASWNU010000000
VERSION     DASWNU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14535"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2280)
                     /locus_tag="VGB57_07330"
     CDS             complement(<1..2280)
                     /locus_tag="VGB57_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0131200.1"
                     /translation="MRKFGLLGTSALGSFTVIGLSLALVSPAYAQTPGQEDDEAQVGQ
                     SEPEIESGTEADTGAQGENATAATETGESITITGSRIRRPNLDSAVPITSIGGEEFFE
                     TGQVSIGDVLNELPQLRSTFSQANSTRFLGTRGLNLLDLRGLGTQRTLVLQNGRRHVP
                     GDVLNNAVSPDVNTFPTDLIERVDLITGGGSSIYGSDAIAGTVNFILKSNYEGFQVRG
                     QSGISKYGDAANQYVSVLAGRNFAGDRGNIAVNLEYARQNDYYVNTRPIREVNGFIVV
                     DTDPAGSVNGSDGNPDRIFVRDTRATTPVGGSVFIRQAAGTPGAGLDPTGAAYSLANI
                     FNPDGSLSTQTGARIGLAPIGTFVGGNAPTGREGNLLALSPQLDRYSINVIGHFEFSP
                     AVVPFIEAKYVRSDAFGSQSGPFFSQGQTLADGINVPGFNDRSYAGAGTTAANTVVNR
                     EGIRLDNPFLSPAARAAIAAQLATAVNANTNPNTGGAIDNATTGTTNRTRALAQIADG
                     SFRFNLRKNFAEFGIRDEQFRRETYRAVVGVRGDFNDDWNYEISANYGEHRESNLIIG
                     NINRQRFVLANDAVVNPANGQIVCRSQIDPRFTGTDRAVPTPNPAQLAADVAACVPLN
                     PFGSGASSQAVRDYLLVDSQAEGKITQFVLSGFVAGDLSQLFELPGGAIGFSLGAEYR
                     RETVFYDLDDITQLGYAFYNAIPTLDPPSFKVKEVFGEISIPLLKDMPFFQELTITGS
                     GRISDYNTSAGTTYAYGGEI"
     gene            complement(2469..3650)
                     /locus_tag="VGB57_07335"
     CDS             complement(2469..3650)
                     /locus_tag="VGB57_07335"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0131201.1"
                     /translation="MPSSTDVVITAAKRTPVGSFLGAFASTPAHELGRIAILAALEQA
                     GVAPEEVSEVILGQVLTAAQGQNPARQASMAAGVPKEVPAWGVNQVCGSGLRAVALAS
                     QAIQTGDASIVIAGGQESMSLSAHAQHLRGGTKMGGLELIDTMIRDGLTDVFNGYHMG
                     ITAENLAEQYQVTRDDQDAFAAASQNKASKARSEGRFRDEIAAVTVKGRKGDTIVEND
                     EYIRDGVEQSALAGLRPAFRKDGTVTAGNASGLNDGAAALVVMSRGEAERRGARILAR
                     VASWASAGVDPSIMGIGPVPATQRALQKAGWSVGDLDLIEANEAFAAQALAVGKELGW
                     DPDKVNVNGGAIAIGHPIGASGARVLTTLLYEMGRRDSRKGLATLCIGGGMGIALCVE
                     R"
BASE COUNT          603 a         1178 c         1316 g          642 t
ORIGIN      
        1 aatctcgccg ccataagcgt aggtcgtgcc cgcggacgtg ttgtagtccg agatacggcc
       61 cgagccggtg atggtcagct cctggaagaa cggcatgtcc ttcagcagcg ggatcgagat
      121 ttctccgaac acttccttca ccttgaacga aggcggatcg agcgtcggga tggcgttgta
      181 gaaggcgtaa cccagctgag tgatgtcgtc gagatcgtag aagacggtct cgcgacgata
      241 ttcggcaccc agagagaagc cgatcgcgcc gccgggcagc tcgaaaagct ggctcaggtc
      301 gcctgcgacg aagccgctga ggacgaactg ggtgatcttg ccttcggcct gggagtcgac
      361 cagcagataa tcccgcaccg cctgcgagga agcccccgag ccgaacggat tgagcggaac
      421 gcaggccgcg acgtcggcgg cgagctgggc cgggttcggg gtcggcacgg cgcgatccgt
      481 gccggtgaag cgcggatcga tctgcgagcg gcagacgatc tggccgttgg ccggattgac
      541 cacggcatcg ttggccagga cgaagcgctg gcggttgatg ttgccgatga tcaggttcga
      601 ctcacgatgc tcgccgtagt tcgccgagat ctcgtagttc cagtcgtcgt tgaaatcgcc
      661 gcgcacaccg accaccgcgc gataggtctc gcggcggaac tgctcgtcgc ggatcccgaa
      721 ttcggcgaag ttcttgcgca ggttgaagcg gaaggagccg tccgcgatct gagcgagagc
      781 gcgagtccgg ttggtcgtcc cggtagtggc attgtcgatc gcgccgccgg tgttcgggtt
      841 ggtgttggca ttgaccgcgg tggcgagctg ggcggcaatg gccgcacgcg ccgccgggct
      901 gaggaagggg ttgtcgagcc ggatgccttc ccggttcacg acggtgttgg cggcggtggt
      961 ccccgctccg gcataggagc ggtcgttgaa gccgggaacg ttgattccgt cggcgagcgt
     1021 ctgtccctgc gagaagaacg ggccgctctg cgagccgaac gcatcggagc gcacgtattt
     1081 ggcctcgatg aacggaacga cggccggcga aaactcgaag tggccgatca cgttgatcga
     1141 gtagcggtcg agctgcggcg acagagccag gaggttgccc tcgcggccgg tcggagcgtt
     1201 gccgccgacg aacgtgccga tcggggccag gccgatgcgg gcaccggtct gcgtggacag
     1261 cgagccgtcg ggattgaaga tgttggccag cgagtaagcg gctccggtcg gatcgagccc
     1321 ggcacccggg gtgcccgccg cctgacggat gaagaccgag ccgccgaccg gagtcgtcgc
     1381 gcgggtgtcg cggacgaaga tacggtccgg gtttccgtcg gaaccgttca cgcttccggc
     1441 cgggtcggtg tcgacgacga tgaagccgtt gacctcccgg atcggccggg tgttgacgta
     1501 atagtcgttc tggcgggcgt attcgaggtt caccgcgatg ttgccgcgat cacccgcgaa
     1561 gttccggccg gcgaggaccg agacatactg gttggcggca tcgccatatt tgctgatgcc
     1621 ggactggccg cgtacctgga agccctcgta attgctcttc aggatgaagt tgaccgtgcc
     1681 ggcgatggcg tcggagccgt agatcgagga gccgccgccg gtgatcaggt cgacgcgctc
     1741 gatcaggtcc gtcgggaaag tgttcacgtc cggcgaaacg gcgttgttga gaacgtcgcc
     1801 cggaacgtgc cggcggccgt tctgaaggac gagagtgcgc tgcgtgccga gaccgcgaag
     1861 gtcgagcagg ttgaggccgc gggtgccgag gaaccgggtg gagttcgcct ggctgaaggt
     1921 gctgcgcagc tggggcagct cgttcaggac gtcgccgatg gaaacctggc cggtctcgaa
     1981 gaattcctcg ccgccgatgg acgtgatcgg aacggcggaa tcgaggttcg gacgacggat
     2041 gcgcgatccc gtgatggtga tcgattcgcc ggtctccgtg gcggccgtgg cgttctcgcc
     2101 ctgggcgccg gtgtcggctt cggtgccgga ttcgatctcg ggctcgctct ggccgacctg
     2161 ggcctcatcg tcttcctgac cgggggtctg cgcataggcg gggctcacaa gcgcgagcga
     2221 aagaccaata acagtaaagg agccgagcgc gctcgtgccc agcaggccaa attttctcat
     2281 cccgaatcat ccctcttgat cagccgcgct tgctgcgcgc ggcttctgga tgcacgcaga
     2341 gacgattcag cccccccttg caaggtcgag acagcggccg ttaatcgaaa gttcagcgag
     2401 tgtagccgtt ctgcaacata ccgcgataga ggccgtcaaa aaaagcaatt gcggagaagc
     2461 gcttggccct atcgctcgac gcagagcgcg atgcccatgc ctccgccgat gcacaaggtc
     2521 gccagaccct tcctcgagtc ccggcggccc atttcgtaaa gcagagtcgt cagaacccga
     2581 gctccgctcg cgccgatggg atggccgatg gcgatcgccc cgccgttgac gttgaccttg
     2641 tccggatccc agccgagctc cttgccgacc gccagcgcct gcgcggcgaa cgcctcgttg
     2701 gcctcgatca ggtcgagatc gccaaccgac cagcccgctt tctgcaacgc ccgctgcgtc
     2761 gccggcaccg ggccgatgcc catgatcgag gggtcgaccc cggccgaggc ccagctcgcg
     2821 acccgggcga ggatcctggc gccccggcgc tcggcttccc cgcggctcat caccaccaaa
     2881 gcggcggcgc cgtcgttcag gcccgaggcg ttgccggcgg tgaccgtgcc gtccttgcgg
     2941 aaggccgggc ggaggccggc gagcgcggac tgctccaccc cgtcgcgaat atattcgtca
     3001 ttctcgacga tcgtgtcgcc cttgcggccc ttcaccgtga cggcggcgat ctcgtcgcgg
     3061 aaccggcctt cggagcgggc cttcgacgcc ttgttctgcg aggcggcggc gaaggcgtcc
     3121 tgatcgtcgc gggtaacctg atactgctcg gccaggttct cggcggtgat gcccatgtga
     3181 tagccgttga agacgtcggt gaggccgtcc ctgatcatcg tgtcgatcag ctcgagcccg
     3241 cccatcttgg tgccgccgcg caggtgctgg gcgtgggcgg aaaggctcat gctctcctgg
     3301 ccgccggcga tgacgatgct ggcgtcgccg gtctggatcg cctggctcgc cagggccacc
     3361 gcgcgcagcc ccgagccgca cacctggttg accccccagg ccggaacctc cttcggcacc
     3421 cccgccgcca tcgatgcctg tcgggccgga ttctggccct gcgccgcggt cagcacctgg
     3481 ccgaggatga cctcgctcac ctcctccggc gcgactcccg cctgctccag cgccgccagg
     3541 atcgcgatcc ggccgagctc atgggccggg gtcgaggcga aggcgccgag gaaggagccc
     3601 acgggggtgc gcttggcggc ggtgatgacg acgtcggtgg atgagggcat gcgggactcc
     3661 tgggcaaagg ctcgtcatcc cggcgaaggc tgggatctcg gcgggtgaaa ggcgccgtgt
     3721 tcttcacccg ccggggccc
//
LOCUS       DASWNU010000367         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_223001, whole genome shotgun sequence.
ACCESSION   DASWNU010000367 DASWNU010000000
VERSION     DASWNU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223001"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VGB57_07340"
     CDS             complement(<1..1161)
                     /locus_tag="VGB57_07340"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0131202.1"
                     /translation="MYLPVAGLLLLFLYWAATHISFGGGGGAPAQPELGISNQQLANI
                     EERQNAPGSVADYKRLDERLQALMRGQQMVGLAVGVVENGEIRFLKGYGETVAGSGEA
                     VTTDTVFRWASLSKGVAGDMVALLADQGKLSLYEPVAKYSASLRLPAGNEQKATVADL
                     LSHRLGLFSHANDSKLEDGMDPRFLRASLAQLNAICSPGSCWAYQNVAYDAATEVVEK
                     VTGQSYADAVRERLFGPLGMTGASMTREALVTSKRWARPHVGGKNSKPVEVAEPYYRV
                     PAAGGVNSTIKDLTIWMQAQMGLKAGVLSPRVLEAVQTGRVRTPGELGRMRKYRERVR
                     TAAYGLGWRVYDYSGHRVVGHHGGVRGYRSLIMFDPALKSGVVALWNGSSSQP"
     gene            complement(1243..3303)
                     /locus_tag="VGB57_07345"
     CDS             complement(1243..3303)
                     /locus_tag="VGB57_07345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY0131203.1"
                     /translation="MIKYIALCGVAALSLAAASAPAQPAAGHAGHAAAKPHFGEWGFD
                     VAGMDRSVNPGDNFFNFANGRWAATTQIPADKAVWGGFIELDELSTQRTRAIIEDAAR
                     ANAPAGTVQRKVGDFYASLMDEAAIEAKGVAPLKPGLDQIAAIRTSSDLARAFGLLGQ
                     VGIGSPFGVQVDQDLKDNSRYSVYVGQGGLGLPDRDYYLDASNAKFVDAKAKYRTHIA
                     NMLRLAGISDPEGRAGRILDLETRIAQAHWTRVQNRQIENLYNPMTKAELATKMPGFD
                     WNAYLTAAGLGSVDRVVVAQPSALTGAAKLVASEPIDTWKDYLAFRTIAASAPMLPKA
                     FVEENFAFNGKVLSGTPQLRERWKRASGLVGNGMGEAVGQLYVARYFPPEAKAKADEL
                     VRNLIKAMDLRLQGLTWMAPETKAKARVKLAAFTPKIGYPDKWRDYSALEIRQGDALG
                     NAMRANQFEYRRNVAKIGQPIDRSEWGMSPQTVNAYANPLLNEIVFPAAILQPPFFDS
                     NADDAVNYGGIGAVIGHEISHHFDDQGRKFDSKGNLADWWTEKDIAEFKKLTDRMVAQ
                     YGAYEALPGAKVNGQLTLGENIADLAGLAIAYDAYQVSLGGKPAPVIGGYTGDQRFFL
                     GFGQVWRTKYRDALLQQLLTVDSHSPGPIRPLVVRNFDPWYKAFNVRDGKLYLSPEQR
                     IRIW"
BASE COUNT          550 a         1092 c         1193 g          591 t
ORIGIN      
        1 cggctggctg ctcgagccgt tccagagcgc caccaccccg gatttcagcg ccggatcgaa
       61 catgatcagc gagcgatagc ccctcactcc gccgtgatgg ccgacgaccc ggtggccgga
      121 ataatcgtag acccgccagc ccaggccgta ggccgcggtt cgaaccctct cccgatactt
      181 gcgcatccgg ccgagctcgc ccggcgttcg gacccgaccg gtctgcacgg cctcgagcac
      241 ccgcggcgaa aggactccgg ccttgaggcc catctgcgcc tgcatccaga tggtcaggtc
      301 cttgatggtc gaattgaccc cgcccgccgc cggcacccgg taataaggct cggcgacctc
      361 gaccggcttc gaattcttgc ccccgacatg ggggcgggcc cagcgtttcg aggtgaccag
      421 cgcctcgcgg gtcatgctcg ctccggtcat cccgaggggg ccgaagaggc gctcgcggac
      481 ggcatcggca tagctttggc cggtgacctt ctcgacgacc tcggtcgccg cgtcataggc
      541 gacgttctga taggcccagc agcttccggg cgagcagatc gcgttcagct gggcgaggct
      601 ggcgcgcagg aaacgcgggt ccatcccgtc ttccagcttg gaatcgttgg cgtggctgaa
      661 caggcccagg cggtgcgaga gaagatcggc gaccgtcgcc ttctgctcgt tgccggcggg
      721 caggcgaagc gacgccgaat atttggcgac cggttcgtag agcgagagct tgccctggtc
      781 cgcgagcaag gcgaccatgt ctcccgccac ccccttcgag agggaggccc agcggaagac
      841 ggtgtcggtg gtgaccgcct cgccgcttcc ggcgaccgtc tcgccatagc ccttgagaaa
      901 gcggatctcg ccattctcga cgactccgac ggcgaggccg accatctgct ggccgcgcat
      961 cagcgcctga aggcgctcgt cgaggcgctt gtagtcggcg acgctgccgg gcgcgttctg
     1021 gcgctcctcg atattggcga gctgctgatt ggagatgccg agctcgggct gcgccggggc
     1081 gccgccgccg ccgccgaagc tgatatgggt cgctgcccaa tagaggaaca agaggaggag
     1141 accggcgacg ggcaggtaca aaatcggccg cttcagcttt actctcccgc cctactcgcg
     1201 gccgggccgg cgacgacgcg ccggcccggt gcccgacaag ccctaccaga tcctgatacg
     1261 ctgttcaggc gacaaataaa gctttccgtc gcgaacgttg aacgccttgt accacgggtc
     1321 gaaattgcgg acgacgagcg ggcggatcgg gcccggcgag tggctgtcga cggtcagcag
     1381 ctgctggagc agggcgtcgc gatatttggt ccgccacacc tggccgaagc ccaggaagaa
     1441 gcgctggtcg ccggtatagc cgccgatcac cggcgccggc ttgccgccca gcgacacctg
     1501 ataggcgtcg taggcgatgg cgaggccggc gagatcggcg atgttctcgc cgagcgtgag
     1561 ctggccgttg accttcgccc ccggaagcgc ctcgtaggcg ccgtattggg cgaccatccg
     1621 gtcggtcagc ttcttgaact cggcgatgtc cttctcggtc caccagtcgg cgagattgcc
     1681 cttcgagtcg aacttgcggc cctgatcgtc gaaatggtgg ctgatctcgt ggccgatgac
     1741 ggcgccgatg ccgccgtaat tgaccgcgtc gtcggcattg gaatcgaaga agggcggctg
     1801 gaggatcgcc gccgggaaca cgatctcgtt gagaagcgga ttggcgtagg cgttcaccgt
     1861 ctggggcgac atgccccatt cggagcggtc gatcggctgg ccgatcttgg cgacgttgcg
     1921 gcgatattcg aactggttgg cgcgcatcgc gttgccgagc gcgtcgccct gccggatctc
     1981 gagcgcggaa taatcgcgcc acttgtccgg atagccgatc ttcggagtga aggcggcaag
     2041 cttgacccgc gccttggcct tggtttccgg ggccatccag gtcaggccct ggagcctcag
     2101 gtccatcgcc ttgatgaggt tgcggacgag ctcgtcggcc ttggccttgg cttccggcgg
     2161 gaagtagcgg gcgacgtaga gctggccgac cgcctcgccc atgccgttgc cgaccaggcc
     2221 ggaggcgcgc ttccagcgct cgcgaagctg cggagtgccg gacaggacct tgccgttgaa
     2281 ggcgaaattc tcttcgacga aggccttcgg aagcatcggc gccgaagctg cgatggtccg
     2341 gaaggcgaga taatccttcc aggtgtcgat cggctcggag gcgaccagtt tcgccgctcc
     2401 ggtgagggcg ctcggctggg cgaccacgac ccgatccacc gagccgaggc cggcggccgt
     2461 gagataggcg ttccagtcga agccgggcat cttcgtggcg agctcggcct tggtcatcgg
     2521 attgtagaga ttctcgatct gccggttctg gacccgggtc cagtgggcct gggcgatcct
     2581 ggtctcgagg tcgaggatcc gcccggctcg gccttccgga tcggaaatgc cggccagccg
     2641 aagcatgttg gcgatgtgcg tcctgtactt cgccttggcg tcgacgaact tggcgttcga
     2701 ggcatcgaga taataatcgc ggtcgggaag gccgaggccg ccctggccga catagaccga
     2761 gtagcggcta ttgtccttga ggtcctggtc gacctggacg ccgaagggcg agccgatacc
     2821 gacctgtccg agcaggccga aagcgcgggc gagatcggat gaggtgcgga tggcggcgat
     2881 ctggtcgagg ccgggcttga gcggtgcgac gcccttcgcc tcgatcgccg cttcgtccat
     2941 caagctggcg tagaagtcgc cgaccttgcg ctggacggtg cccgcggggg cgttcgccct
     3001 ggcggcgtcc tcgatgatgg cgcgggtgcg ctgggtggag agctcgtcga gctcgatgaa
     3061 gccgccccag acagccttgt cggccgggat ctgggtcgtc gcggcccagc ggccgttggc
     3121 gaagttgaag aaattgtcgc ccggattgac gctccggtcc atccccgcga cgtcgaagcc
     3181 ccactcgccg aaatgcggct tggcggcggc gtgcccggca tggccggcgg ccggctgggc
     3241 cggagcggag gcggccgcga ggctgagcgc ggccacgccg caaagcgcta tgtatttgat
     3301 catgtttgga attccccacc caatctggat taaggcgcca atagcggcca ccaacggatt
     3361 tgggaagggg gcgcgttgca aggccgtgat cccggcgaaa gccgggacct cgggacgaga
     3421 agtctc
//
LOCUS       DASWNU010000368         7922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_482208, whole genome shotgun sequence.
ACCESSION   DASWNU010000368 DASWNU010000000
VERSION     DASWNU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482208"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..672)
                     /locus_tag="VGB57_07350"
     CDS             complement(253..672)
                     /locus_tag="VGB57_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0131204.1"
                     /translation="MALPPFHLAFPVHDLDAARAFYGGLLGCPEGRSADDWIDFDFFG
                     HQIVAHLDRGMERRRHHNPVDGHDVPVPHFGAVLDLAAWQAMADKLKAAGAGFVIEPT
                     IRFRGQPGEQATMFFLDPSGNALELKAMADPENLFAK"
     gene            782..2311
                     /gene="lnt"
                     /locus_tag="VGB57_07355"
     CDS             782..2311
                     /gene="lnt"
                     /locus_tag="VGB57_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0131205.1"
                     /translation="MKPLALSFLAGLVGACGFEPIGLWPLVPMAFGLLLWLVRGAPGL
                     RSALARGWWFGVGHFVVGLNWIATAFTYQAEMPASLGYLAVVLLSLYLAVYPAAAAGL
                     AWRWGRKNDLRLVLVFAAAWIVTEWLRATLFTGFAWNPIGVALLATPLSWSARLVGTY
                     GLSGVTALLAGILLLLCLRRWRSAGAAAAGISILALAAFLAGGPAPGPAGPSLRIVQP
                     NIGQQDKWREGFQEENMDRLRRLSNWGGSEPRLLLWPEAAVTRPLENGLDDPGHIIEM
                     LELRRDVAAMLGEKDLLLTGGVTWRSADGRDVTSATNSVFAIDPAGRILARYDKAHLV
                     PYGEYLPMRPILSSLGLSRLAPGDVDFDPGPGARTLDLPVIGKAGFQLCYEIIFSGEV
                     VDRKNRPAFLFNPSNDAWFGAWGPPQHLAQARLRALEEGLPVLRSTPTGISAVIDPDG
                     RLLRSLPWRTAGIIDTRLPAPRAPTPFARLGNLLPLLFALLLVGLAMVAQRIAGRPKE
                     G"
     gene            2383..3594
                     /gene="metK"
                     /locus_tag="VGB57_07360"
     CDS             2383..3594
                     /gene="metK"
                     /locus_tag="VGB57_07360"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0131206.1"
                     /translation="MRSNYLFTSESVSEGHPDKVSDQISDAVVDLFLGKDPEARVACE
                     TLTTTQKVVLAGEIRGRGIMDEEGNWAPGVPEEIEAVVRETVKAIGYEQDGFHWETLD
                     FSNNLHPQSAHIAQGVDASGNKDEGAGDQGIMFGFACDETPDLMPATLYYSHKILERM
                     AADRHSGAAPFLEPDAKSQVTLRFENGEPVAATAIVVSTQHGKGYDQGEKEAELKAYV
                     RRVVADVLPAELLSDDTVYHINPTGSFEIGGPDGDAGLTGRKIIVDTYGGAAPHGGGA
                     FSGKDPTKVDRSAAYIARYLAKNIVAAGLARRCTIQLAYAIGVSAPLSLYVDTHGTGN
                     GADDAAIERAIMSIQDLGGLTPRAIRTHLGLNKPIYRKTAAYGHFGRTAEGDFFPWEK
                     TDLADRLKAAL"
     gene            complement(3728..4096)
                     /locus_tag="VGB57_07365"
     CDS             complement(3728..4096)
                     /locus_tag="VGB57_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131207.1"
                     /translation="MRYAIAAAALCCSAPAFATGGFLCSPAGGKEPAISLVIGHGIPG
                     GVVGAAIREDGRWRSTMQPGEGLVLDQWWIDRERTWVDLVHPETGAPEAQLRVRNQGF
                     GGTGTLVRKGRTYQVRCFQD"
     gene            4120..4800
                     /locus_tag="VGB57_07370"
     CDS             4120..4800
                     /locus_tag="VGB57_07370"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY0131208.1"
                     /translation="MNDPSTIRRLYGRRSGHKLRGGQEALLERLLPAVSVPEEGPVTS
                     EALFGDSRPLHFEIGFGSGEHLAYRADLLPDHGFVGAEPFLNGVVGALQHIRDQRLAN
                     VRLHMGDALDVLERVPDGVLTMVYLLHPDPWPKARHAKRRMVNPGPLDLIAAKLRPGG
                     EFRLGTDDPTYCRWATMVMNGRRDFEWLAESARDFLTRPGGWPETRYEAKARRKGHEV
                     WYFRYRRV"
     gene            4844..5728
                     /locus_tag="VGB57_07375"
     CDS             4844..5728
                     /locus_tag="VGB57_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131209.1"
                     /translation="MVLRRAALSEPDRRFIIARSGGCCNKCRTQVFRENEFAEKARIG
                     DDAHIWAYSAEGPRGGAPGAPDDRNARENIILLCKNCHAEVDQQPIKFSAAMLTALRE
                     SHYAWVDSCLGEIRAEKPRFHYILYLNVPRVDMYAVANSIALPRLEFGNARRFKDLGF
                     EAGRVMATYTHILNAEDLYAHQVGNDDDISCLRIGQYCFIAPANFRTVAIGKSGDLNV
                     AWASDRSTVYCRFADWTLVCLIDPRWITTSTAGSTLESGHARLCGVVRISRTDAEARK
                     VYASPLFLAQPGGSASDY"
     gene            complement(6048..7187)
                     /locus_tag="VGB57_07380"
     CDS             complement(6048..7187)
                     /locus_tag="VGB57_07380"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0131210.1"
                     /translation="MRSLIPLLLLAACSAGGEAAESNAEAATQAGGGRPFTVRQITIF
                     DEPWAMAVLPGPGQRALITEKKGKLKLLWSEVGRVGDVAGAPKVDYGGQGGLGDVVLH
                     PGFARNRLVYLSWVEAGQSGNRGAVVGRARFVEDGGRPRLEGLQVIYRQSPKLGGKGH
                     YGHRIAFGPDGKLYISNGDRQEFTPAQDMSGNLGKIVRLNDDGSVPADNPFASRGGVT
                     AQIWSLGHRNPLGIAFDASGRLWDIEMGPKGGDELNLVERGKNYGYPIVSNGDHYDGK
                     DIPDHRTRPEFAAPRVSWTPVISPGNMIFYSGSAFPAWRGDILVAGLSSKALIRVDVN
                     GANAREAERWDMGKRIREVEQGPGGSVYLLEDGEGGRLLKLTPAR"
     gene            7310..7888
                     /locus_tag="VGB57_07385"
     CDS             7310..7888
                     /locus_tag="VGB57_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131211.1"
                     /translation="MKLAAPLLAGLLAGCATPAVDEAPRDRFFGSLASLCGKAFEGRI
                     ASPPVEADSAFAGKRLVMHVRGCTKDEIRIPFHVGEDRSRTRVVSRAGTGLRLKHDHR
                     HTDGSEDELTRYGGDTVAAGTARRQEFPADDASKSLFTRENIPASTANVWAMETDGRM
                     FAYELRRAGRFFRVEFDLTRPVAPPPAPWGSG"
BASE COUNT         1256 a         2693 c         2634 g         1339 t
ORIGIN      
        1 caggaaagtc gcggttttca ggctgttccc catttgacat gccccttgtt gtttatcgcg
       61 tgtcgaggac aggtttgcgc aggcgatcac caacaaatgg ttaacgcggt ttacaccatc
      121 aaattcctcc cccggttttc cgggggaggg ggaccgcgcg aagcgtggtg gaggggttct
      181 tcagcaaggc ccctccacca ggcttcgcct ggtccccctc cccggcaaat gccggggagg
      241 aattcaagca ccttacttcg cgaacaaatt ctccggatcg gccatggcct tgagctcgag
      301 cgcgttgccg gacgggtcga ggaagaacat cgtcgcctgc tcgccgggct ggccgcggaa
      361 gcggatcgtc ggctcgatga cgaacccggc gccggcggcc ttcagcttgt ccgccatcgc
      421 ctgccacgca gccaagtcga ggacggcgcc gaaatgcggc accggcacgt cgtgcccgtc
      481 gaccgggtta tgatggcgcc gcctctccat tccacggtcg agatgggcga cgatctggtg
      541 accgaagaaa tcgaagtcga tccagtcgtc tgcgcttcgg ccttccgggc agccgagcag
      601 tccgccgtag aaagcgcggg cggcgtcgag atcgtggacc gggaaggcga ggtggaacgg
      661 gggcagggcc atgccgcttc caatgtcatt gcggaggatg tgacgcaatg tagcgggatg
      721 tggatttttc tggatgtttc tgggattgct tcgactctaa gctcctcgca atgacggggt
      781 cgtgaagccc ctcgctctct ccttcctcgc cggcctcgtc ggcgcctgcg gattcgagcc
      841 gatcgggctc tggccgctgg ttccgatggc gttcggcctc ctcctttggc tggtgcgtgg
      901 agcgccgggc ctgcgcagcg cgctcgcccg gggctggtgg ttcggagtcg ggcatttcgt
      961 ggtcggcctg aactggatcg ccaccgcctt cacctaccag gccgagatgc cggcgtcgct
     1021 cggctatctg gcggtcgtcc tgctctcgct ctacctcgcc gtctatccgg ccgccgcggc
     1081 cggcctggcc tggcgctggg gacgaaagaa cgaccttcgg ctggtcctgg tcttcgccgc
     1141 cgcctggatc gtcaccgaat ggctgagggc gaccctgttc accggcttcg cctggaatcc
     1201 gatcggggtg gccctcctcg cgacgcctct gtcctggtcg gcgcgccttg tcggcactta
     1261 cggcctttcc ggagtcacgg ccctgctcgc cggcattttg ctcctcctct gcctgcggcg
     1321 ctggcgctcg gccggggcgg cggcggccgg aatctcgata ctggccctgg ccgccttcct
     1381 cgccgggggc ccggcgccgg gccccgctgg accttcgctt cgaatcgtcc agcccaatat
     1441 cggccagcag gacaaatggc gggagggttt ccaggaagag aatatggatc ggctccggcg
     1501 cctctccaac tggggcggaa gcgagccgcg gctcctgctc tggccggaag cggcggtgac
     1561 gaggccgctc gagaacggcc tcgacgaccc cggccatatc atcgagatgc tggagcttcg
     1621 ccgcgacgtc gccgccatgc tcggcgagaa ggaccttctc ctcaccggcg gagtgacctg
     1681 gcgctcggcc gacgggcggg acgtgaccag cgccaccaac agcgtcttcg ccatcgaccc
     1741 ggcggggcgg atcctcgccc gctacgacaa ggcgcatctc gttccctatg gcgaatatct
     1801 gccgatgcgg ccgatcctct cctcgcttgg actgtcgcgc ctggcgccgg gcgacgtcga
     1861 tttcgatccg ggcccgggcg cccggacgct cgacctgccg gtcatcggca aggccgggtt
     1921 ccagctctgc tacgagatca tcttctcggg cgaggtggtc gaccgcaaga accgtcccgc
     1981 cttcctgttc aatccttcca acgacgcctg gttcggcgcc tgggggccgc cgcagcatct
     2041 cgcccaggcc cggctccggg cgctcgagga ggggcttccg gtccttcgct ccactccgac
     2101 cggcatctcc gcggtgatcg acccggacgg gcggctcctc agaagcctgc cgtggcggac
     2161 ggcggggatc atcgacacga ggctgccggc gccgagggcg ccgacgccct tcgcccggct
     2221 gggcaatctc ctcccgctgc tgttcgcact tcttttggtt ggtctggcga tggtcgcgca
     2281 gcgcattgca gggcggccaa aagagggcta gggagcgcac atataaggat atctttatat
     2341 ccttacgtcc cccttgagag ttcaggaagg ccggttctcc cgatgcgcag caattatctc
     2401 ttcacgtccg aatccgtgtc cgaaggccat cccgacaagg tgtccgacca gatttccgac
     2461 gccgtcgtcg acctcttcct cggcaaggac ccggaggcgc gggtcgcctg cgagaccctg
     2521 acgacgaccc agaaggtcgt ccttgccggc gagatccggg gccgcgggat catggacgag
     2581 gaaggcaatt gggcgccggg cgtgccggag gagatcgagg cagtcgtccg cgagacggtg
     2641 aaggcgatcg gctacgagca ggacgggttc cactgggaga ctctcgactt ctcgaacaac
     2701 ctccatcccc agtccgccca catcgcccag ggcgtcgacg cttcgggcaa caaggacgag
     2761 ggcgccggcg accagggcat catgttcggc ttcgcctgcg acgagacccc ggacctgatg
     2821 ccggcgaccc tctattacag ccacaagatc ctcgagcgca tggccgccga ccgccattcg
     2881 ggcgccgcgc ccttcctcga gcccgacgcc aagagccagg tcaccctgcg cttcgagaat
     2941 ggcgagccgg tcgccgcgac cgcgatcgtc gtctcgaccc agcacggcaa gggctacgac
     3001 cagggcgaga aggaggccga gctgaaggcc tatgtccgcc gggtcgtcgc cgacgtgctt
     3061 ccggccgagc tcctctcgga cgacaccgtc taccatatca accccacggg cagcttcgag
     3121 atcggcggac cggacggcga cgccggcctc accggccgca agatcatcgt cgacacctac
     3181 ggcggagcgg ccccccacgg cggcggcgcc ttctccggca aggacccgac caaggtcgac
     3241 cgttcggccg cctatatcgc gcgctacctc gccaagaaca tcgtcgccgc gggcctcgcg
     3301 cgccgctgca ctatccagct cgcttatgcg atcggagtgt cggcgccgct gtcgctctac
     3361 gtcgacaccc acggcaccgg caacggcgcc gacgacgccg cgatcgagcg ggcgatcatg
     3421 tcgatccagg atctgggcgg cctcaccccg cgcgcgatcc ggacccatct cggcctcaac
     3481 aagccgatct acaggaagac cgccgcctac ggccatttcg gccggaccgc ggagggcgat
     3541 ttcttcccgt gggagaagac cgacctggcc gaccggctga aggcggcgct ttaggcctgt
     3601 tggcgggtcg aggccccaaa ccgtcattcc ggcgaaggcc ggaatccagg aacgctggcg
     3661 gcttcaggct cgtgccgcgg tgttcatgga tcccggcctt cgccgggatg acgggatagg
     3721 gccccggcta gtcctggaag cagcgcactt gataggtgcg gcccttgcgg acgagggtgc
     3781 cggtcccgcc gaagccctgg ttgcggaccc ggagctgagc ctcgggggcg ccggtctccg
     3841 gatggacgag gtcgacccag gtccgctcgc ggtcgatcca ccactggtcg aggacgagcc
     3901 cttccccggg ctgcatcgtc gagcgccagc ggccgtcttc cctgatggcg gcgccgacga
     3961 ccccgccggg gatgccgtgg ccgatcacga ggctgatcgc cggttccttg ccgcccgcag
     4021 gcgagcacag gaacccgccg gtggcaaagg cgggcgcgga gcagcacaaa gctgcggcgg
     4081 cgatggcgta tctcatgggc cgaggataaa ggcctcgacg tgaacgatcc cagtaccatc
     4141 cgccgcctct acggccgccg ctccgggcac aagcttcgcg gcggccagga ggcgctgctc
     4201 gagcggctgc ttccggcggt gagcgttccg gaggaggggc cggtcacctc cgaagccttg
     4261 ttcggcgact cgcggccgct ccatttcgag atcggcttcg gctcgggcga gcatctcgcg
     4321 taccgcgccg accttctgcc cgaccacggc ttcgtcggcg ccgagccctt ccttaacggc
     4381 gtcgtcgggg cgctccagca tatccgcgac cagcggctgg ccaatgtccg cctgcacatg
     4441 ggcgacgcgc tcgacgtgct cgaacgggtg ccggacgggg tcttgaccat ggtctacctg
     4501 ctccatcccg atccctggcc gaaggcgcgc cacgccaagc ggcgaatggt caatccgggc
     4561 ccgctcgacc tcatcgccgc caagctcagg ccgggcggcg agttccgcct cggcaccgac
     4621 gatccgacct attgccgctg ggcgacgatg gtgatgaacg gccgccgcga cttcgaatgg
     4681 ctggccgaaa gcgccaggga cttcctcacc cgccccggcg gctggcccga gacccgctac
     4741 gaggccaagg cgaggcgcaa gggccacgaa gtctggtact tcaggtaccg gcgggtgtga
     4801 tgcgcattcg cgagattatg gcgcagaaaa ggcgaggtat tgaatggtcc taaggcgagc
     4861 ggccctgtcg gagcccgacc ggcggttcat catcgcgcgc tccggcggct gctgcaacaa
     4921 atgcaggacc caggttttcc gggagaatga attcgccgag aaagccagga tcggcgacga
     4981 tgctcacatc tgggcttatt ccgcggaagg gccacggggc ggtgcccccg gcgctcccga
     5041 tgaccggaac gctcgggaga atatcatcct tctgtgcaag aattgccacg ccgaggtcga
     5101 tcaacagccc atcaagtttt cggcggcaat gctcacggcg ttacgcgaaa gccattatgc
     5161 gtgggtggat tcctgccttg gtgagatccg ggctgagaaa ccccgcttcc actatatcct
     5221 ctatctcaat gtgcctcggg tagacatgta tgcggtcgcg aattcgattg ccctgccgcg
     5281 cctggagttc ggcaacgcgc gtcgtttcaa ggatctcggg ttcgaggccg gccgagtgat
     5341 ggccacctat acccatatcc tcaacgccga agacctttat gcccatcagg tagggaatga
     5401 tgacgacatt tcctgcctcc ggatcggcca atattgcttc atcgcaccgg ccaacttccg
     5461 taccgttgcg atcggcaaga gtggcgattt gaacgtcgcc tgggcgtcag acaggagcac
     5521 cgtctattgc cgattcgccg attggacact ggtctgcctg atcgacccca gatggatcac
     5581 gacgtccacg gcaggatcga ctcttgaatc tggacacgcg cggctgtgcg gagtcgttcg
     5641 gatcagccgg accgacgccg aggcccgaaa ggtctatgcc tcgccattgt ttctcgcgca
     5701 gccgggagga tccgccagtg actattgatc acgccatggc tcagaggcac ttcgccgcct
     5761 acgacaacgg ccaacccctc ggcgaagacg gaccggtctg gcacggcccg atcgaaaatt
     5821 gcgacgtctg ttcccggccg atgggatcgg aaatctacat gatcgatgga ccctcggaga
     5881 ggtcaccgcg gccgaggtgg ggtaatcttt gcgccacctg cgctttcaag cacagccccg
     5941 ccatagggtg gggaaaggcc cagctttaca agcgccacgg ctcgcaatgg cgtttgctgg
     6001 cgggtggtcc gccacccgac gaggattacg acctttaggg ggttggctca ccgcgccggc
     6061 gtcagcttga gcaggcggcc gccctcgccg tcctcgagca ggtagaccga gccgcccggc
     6121 ccctgctcga cctcgcggat gcgcttgccc atgtcccagc gctcggcctc gcgggcgttg
     6181 gcgccgttga cgtcgacccg gatcagggcc ttgctcgaca ggcccgcgac gaggatgtcg
     6241 ccgcgccagg cggggaaggc cgagcccgaa tagaagatca tgttgccggg cgagatgacc
     6301 ggagtccacg aaaccctggg cgccgcgaat tcggggcggg tccggtggtc gggaatgtcc
     6361 ttgccgtcat aatggtcgcc gttggagacg atcgggtagc cgtaattctt tccccgctcg
     6421 accaggttca gctcgtcgcc gcccttcggg cccatctcga tgtcccacag gcggcccgaa
     6481 gcgtcgaacg cgattccgag cgggttgcgg tgccccaatg accagatctg ggcggtgact
     6541 ccgccccgcg acgcgaacgg attgtcggcg gggacggagc cgtcgtcgtt cagccggacg
     6601 atctttccga gattgccgct catgtcctgg gccggggtga attcctgccg gtcgccgttg
     6661 gagatgtaga gcttgccgtc cggaccgaag gcgatgcggt ggccgtaatg gcctttgccg
     6721 cccagcttcg ggctctgccg gtaaatgact tgcagaccct cgagccgggg gcggccgcca
     6781 tcctcgacga agcgcgcgcg gccgacgacg gcgccgcgat tgccgctttg gcccgcctcg
     6841 acccagctca gatagaccag ccggttccgg gcgaacccgg gatgaaggac gacgtctccc
     6901 aagccgcctt ggccgccgta atcgaccttc ggagcgccgg cgacgtcgcc gacccggccg
     6961 acttcgctcc acagcaattt cagcttgccc ttcttctcgg tgatcaaggc gcgctggccg
     7021 gggccgggca ggaccgccat cgcccagggc tcgtcgaaga tggtgatctg gcggacggtg
     7081 aagggccggc cgcccccggc ctgcgtggcc gcttcggcat tgctctccgc cgcttcgccg
     7141 ccggcgctgc aggcggcgag gaggagaagg gggatgaggc tgcgcatcga tagactcccg
     7201 taagttgccg ccgccctagc ccgagcggcg gggcgcgggc aatcggcaag cgacggaatc
     7261 ccggcccctt ccgctggcgg gcggcgcccg ttcaggctag cgtcgcggca tgaagctcgc
     7321 cgcccccttg ctcgccggcc tgctcgccgg ctgcgcgacg ccagccgtcg acgaggcgcc
     7381 gcgggaccgt ttcttcggca gcctcgcctc gttgtgcgga aaggccttcg agggacggat
     7441 cgcgagtccc ccggtcgagg ccgattccgc tttcgccggc aagcggctgg tgatgcacgt
     7501 ccgaggctgc accaaggacg agatccggat ccccttccac gtcggcgagg accgttcgcg
     7561 gacccgggtc gtaagccgag cggggaccgg cctgcgcctc aagcacgacc atcgccacac
     7621 cgacggaagc gaggacgagc tcacccgata tggcggcgat acggtcgcag cgggaacggc
     7681 gcggaggcag gagttccccg ccgacgatgc ctccaagtcg ctgttcaccc gcgagaacat
     7741 ccccgcctcg acggcgaacg tgtgggcgat ggagacggac ggccggatgt tcgcctacga
     7801 gctgcgccgg gcgggacgct tcttccgggt cgagttcgac ctgacccggc cggtggcgcc
     7861 gccgccggcg ccgtggggct cgggctaaga aatcctcccc tgcatttgca ggggaggggg
     7921 ac
//
LOCUS       DASWNU010000369         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_299066, whole genome shotgun sequence.
ACCESSION   DASWNU010000369 DASWNU010000000
VERSION     DASWNU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299066"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGB57_07390"
     CDS             <1..340
                     /locus_tag="VGB57_07390"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEY0131212.1"
                     /translation="LAARRFPGREVADGVLIIIGGTLLLTPGFVTDVFGLFLLLPPTR
                     AIARRVLKRMTIGRFMVVGVGGGPGPFGGPPGGEDGRRSDRDYDFEGSAEEVPPQGDS
                     DPRLPRSDRS"
     gene            342..863
                     /locus_tag="VGB57_07395"
     CDS             342..863
                     /locus_tag="VGB57_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0131213.1"
                     /translation="MGDGERQTQRLLARRPTPEDRDAYHLHFMHPTVERWLRPPPLPP
                     FDTKVLDDLLEGDRAHWTDHGFGPWVLLDKESGEFAGRGGLAWTSVEGTAQVELPWSI
                     EPRLHRQGLATEAAIAAIEWARELQIEEVVALVLPANVASQRVARKTGFEESGEVPHA
                     GLPHLLFRLKPGD"
     gene            complement(916..1248)
                     /locus_tag="VGB57_07400"
     CDS             complement(916..1248)
                     /locus_tag="VGB57_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131214.1"
                     /translation="MNRSRAFKLLLLALALSLSAVPSASASAEQSAVCHTVAQSVTER
                     KNISCKQARKVATRAGQALKGFPECPGDDIRIWNGWRLTPDPAGVAIGTRFKKGDRSF
                     HLTGGGAC"
     gene            complement(1265..1624)
                     /locus_tag="VGB57_07405"
     CDS             complement(1265..1624)
                     /locus_tag="VGB57_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY0131215.1"
                     /translation="MPENPAATDQPAEVKADQHDETLTEEDVYEALEEVIDPELGLDF
                     VSLGLVYDVNIEGPEVYVTFTLTTPACPIGPQVSEQMREFVGDLPGVTAVHPKMIFDP
                     AWSPEMMTEDAKVALGF"
BASE COUNT          245 a          573 c          591 g          257 t
ORIGIN      
        1 gctggcggcg cggcgcttcc ccggccgcga ggtcgccgac ggggtgctaa tcatcatcgg
       61 cggcaccctg ctgctgacgc cgggcttcgt caccgacgtc ttcggcctct tcctgctgct
      121 gccgccgacg cgggcgatcg cccggcgggt gctgaaacgg atgacgatcg ggcgcttcat
      181 ggtcgtgggc gtcggcggcg gcccaggccc gttcggcggt ccgcccggcg gcgaggacgg
      241 gcggcgctcg gaccgtgact acgacttcga gggctccgcg gaagaggtgc cgccgcaggg
      301 cgactccgac ccccggctac cgcgctcgga ccggagctga gttgggggac ggggagcggc
      361 agacgcagcg cctcctcgcc cgccggccga cccccgagga ccgcgacgcc taccacctcc
      421 acttcatgca cccgacggtc gaacgctggc tgcgcccccc tcccctgccg cccttcgaca
      481 ccaaggtcct cgacgatctg ctcgagggcg accgcgccca ctggaccgac cacggcttcg
      541 gcccctgggt cctgctcgac aaggagagcg gcgagttcgc cggccgcggc ggcctcgcct
      601 ggacctcggt cgagggaacc gcccaggtcg agctcccctg gagcatcgag ccccgcctgc
      661 accgacaggg gctggcgacc gaggcagcga tcgctgccat cgagtgggcg cgggagctgc
      721 agatcgagga ggtggtcgcg ctggtcctcc ccgccaacgt cgcctcccag cgggtggccc
      781 gaaagacggg gttcgaagag agcggcgagg tgccccacgc cggcctgccg cacctgctgt
      841 tccggttgaa gccgggtgat tgacgcttcc cggtgccgcc atcccctccg gcgcaccgct
      901 acagccgcaa agcgatcagc aggcgccgcc gccggttagg tggaaagagc gatcgccctt
      961 cttgaagcga gtcccgatcg cgactccggc aggatcaggc gtcagtcgcc aaccgttcca
     1021 aatacggatg tcgtcgccgg gacactcggg gaatcccttg agcgcctgcc cggcccgcgt
     1081 cgcgactttg cgtgcttgtt tgcaggagat gttcttccgc tccgtaacgc tctgggcaac
     1141 ggtgtgacaa acggcggact gctcggccga tgcagaggcg ctcgggactg ccgacagcga
     1201 gagggccaaa gccaacagca ggagcttgaa ggcacggctg cgattcatgg cgccttaaac
     1261 cttcctagaa ccccagcgcg accttggcgt cctcggtcat catctccggg ctccaggcgg
     1321 ggtcgaagat catcttcggg tgcacggcgg tgacgccggg gaggtcgccg acgaactcgc
     1381 gcatctgctc ggagacctgg gggccgatcg ggcaggcggg ggtcgtcagg gtgaaggtga
     1441 cgtagacctc gggaccctcg atgttgacgt cgtagacgag gccgagggag acgaagtcga
     1501 ggccgagctc ggggtcgatg acctcctcga gcgcctcgta gacatcctcc tcggtgaggg
     1561 tttcgtcgtg ttggtcggcc ttgacctcgg ccggctggtc ggtggctgcg ggattctcgg
     1621 gcatcgccta atggtagccg ggcgctaaca cggctccgcg ggcggt
//
LOCUS       DASWNU010000370         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_376666, whole genome shotgun sequence.
ACCESSION   DASWNU010000370 DASWNU010000000
VERSION     DASWNU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376666"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1151)
                     /gene="pip"
                     /locus_tag="VGB57_07410"
     CDS             complement(204..1151)
                     /gene="pip"
                     /locus_tag="VGB57_07410"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY0131216.1"
                     /translation="MTELRTLYPPIQPYETGRLDVGDGHSLYWERCGTRGAKPVVFLH
                     GGPGAGCGPDHRRQFDPARYDILLFDQRGCGRSTPHASLEANTTWHLVDDIERLRELA
                     GHESWMVFGGSWGSTLALAYAETHPGRVTELVLRGIFTFRQTELHWLYLYGASEVFPD
                     KWRDFLAPIPEAERDDLVGAYQRRLTGDDPEARLAAAKAWSQWEAETVTLLPSPATIA
                     AHTSDDFAVAIARIENHYMANRGWLDEGQLIADAGRLKDIPAVIVQGRYDMCTPPSAA
                     WDLHEAWPEARLEIVHGGHLFNEPEVLDGLIRASDGFAG"
     gene            complement(1276..2649)
                     /locus_tag="VGB57_07415"
     CDS             complement(1276..2649)
                     /locus_tag="VGB57_07415"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY0131217.1"
                     /translation="MTTRTETARADPPIESRDDLLAPFAKGEKPQSAWRIGTEHEKFV
                     YRRSDHRAPSYDEPGGIRDLLDALTEFGWERVEEGGKVIALSGKDGNVSLEPAGQLEL
                     SGAALENLHQTCAEAGRHLDQVKQVGDKLGLGFLGLGMWPDKSRDELPRMPKGRYEIM
                     LRHMPRVGSLGLDMMLRTCTIQVNLDYSSEADMAQKFRVGLALQPLATALFANSPFTE
                     GKPNGFLSFRSHIWSDTDGARTGMLPFVFEDGFGYERYIDYALDVPMYFVYRDGRYID
                     AAGLSFRDFLKGELSVLPGEKPRLSDWTDHLSTAFPEVRLKSFLEMRGADGGRWSKIC
                     ALPALWVGLLYDRSALDAAWDEVRHWSLDERQRLRDEVPKLGLAARTPGGETLRELAG
                     RILAIAESGLNSRARFNGAGDSESGFLDPLREIVTRGTTPAEMLLERYRGDWQGDVGR
                     IYEEMSF"
     gene            complement(2738..>3373)
                     /locus_tag="VGB57_07420"
     CDS             complement(2738..>3373)
                     /locus_tag="VGB57_07420"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY0131218.1"
                     /translation="NVLRLGPGAGVKLFDDRTGEWLAEVAEAGKKRVTLAVTRHLRAR
                     EPVPDLWLLFAPIKRGRIDWLVEKATELGVARLVPVLTRRTVVERLNLDRLRAHIVEA
                     AEQCERTSLAELAEPQKLGALLAGWPGERILHFADEAGGEPFTAEPGPAAILIGPEGG
                     FTEEERAAIRALPQARPISLGPRILRADTAALAAVTLWMGAAGDWTASSRA"
BASE COUNT          523 a         1103 c         1207 g          540 t
ORIGIN      
        1 tcgccgcact tcaacgacca ggccgagcgc tacgcgaccg gcaacctacg ccaggtctat
       61 ttctatccgg agcagctgaa ggggcatacg gaaagggtgt accggccggg ccagtagccg
      121 tcattgcgag gagcgtagcg acgaagcaat ccagcgggcg tcggaggcag cactggattg
      181 cctcgcttcg ctcgcaatga cggctatccg gcgaacccgt cgcttgccct tatcagcccg
      241 tcgagcacct cgggctcgtt gaacagatgg ccgccgtgga cgatttcgag cctcgcctcg
      301 ggccaggctt cgtgcaggtc ccaggccgcg gagggcgggg tgcacatgtc gtagcggccc
      361 tgcacgatca ccgccggaat gtccttcagc cggccggcat cggcgatcag ctggccttcg
      421 tcgagccagc cgcgattggc catgtagtga ttctcgattc gggcgatggc gaccgcgaaa
      481 tcgtcgctgg tgtgggccgc gatcgtggcc gggctgggca gcagggtgac ggtctccgcc
      541 tcccactggc tccacgcctt ggcggccgcg agccgggcct ccggatcgtc gccggtgagg
      601 cggcgctgat aggccccgac cagatcgtcg cgctccgcct ccggtatcgg cgcgaggaaa
      661 tcccgccact tgtccggaaa gacttcggac gcgccataaa gatagagcca gtggagttcg
      721 gtctggcgga aggtgaagat gccgcgcagc acaagctcgg tgacccgccc gggatgggtc
      781 tcggcataag cgagggcgag cgtcgagccc caggagccgc cgaacaccat ccagctttcg
      841 tggcccgcca gctcccgcag ccgctcgatg tcgtcgacga gatgccaggt cgtattggcc
      901 tcgagggagg cgtggggggt ggagcggccg cagccgcgct ggtcgaagag gaggatgtcg
      961 tagcgggccg ggtcgaactg gcggcggtga tcggggccgc agccggcgcc ggggccgccg
     1021 tgaaggaaga cgaccggctt ggcgccgcgg gtgccgcagc gctcccaata gaggctgtgg
     1081 ccgtcgccga cgtcgaggcg gccggtttca tagggctgga tcggagggta gagggtgcgt
     1141 agctcggtca tttcggatca ctcctgcgcg acgtcctgag ccgtcattgc gaggagcgca
     1201 gcgacgaagc aatccaatac gccgcgtggg tcgtaagcag cgctggattg cttcgctacg
     1261 ctcgcaatga cggagtcaga agctcatttc ctcgtagatt cggccgacgt cgccctgcca
     1321 atcgccacga tagcgttcga gcagcatctc ggccggcgtg gtgcctcggg tgacgatctc
     1381 ccgcagaggg tcgagaaagc cgctctcgct gtcgccggcg ccgttgaacc gcgcgcgcga
     1441 attcaggccg ctttcggcga tggcaaggat tcgccccgcg agctcgcgca gcgtctcgcc
     1501 gccgggcgtg cgcgcggcga ggccgagctt cggcacctcg tcgcgaagcc gctggcgctc
     1561 gtcgagcgac cagtgcctga cctcgtccca ggcggcatcg agcgccgacc ggtcgtagag
     1621 cagcccgacc cagagcgcgg gcagggcgca gatcttgctc cagcggcctc cgtcggcgcc
     1681 gcgcatctcg aggaagctct tgagccgcac ctccggaaag gcggtggaga gatggtcggt
     1741 ccagtcggac aggcgcggct tctcgccggg aaggacggaa agctcgccct tgaggaagtc
     1801 gcggaaggag aggccggcgg cgtcgatgta gcggccgtcg cggtagacga agtacatcgg
     1861 cacatcgagc gcatagtcga tgtagcgttc gtagccgaag ccgtcctcga agacgaaggg
     1921 cagcatcccg gtgcgggcgc cgtccgtgtc ggaccagatg tggcttcgga atgacaggaa
     1981 gccgttgggc ttgccttcgg tgaagggcga gttggcgaag agggcggtgg cgagcggctg
     2041 cagggccagg ccgacccgga acttctgcgc catgtcggct tccgagctat agtccaggtt
     2101 aacctgtatg gtacaggtgc ggagcatcat gtcgagcccg aggcttccga cccgaggcat
     2161 gtggcggagc atgatctcgt agcggccctt gggcattcgc ggaagctcgt cgcggctctt
     2221 gtcgggccac attccgaggc cgaggaagcc gaggccgagc ttgtctccca cctgcttgac
     2281 ctggtcgaga tggcggcccg cctccgcgca ggtctggtgg aggttttcca gcgcggcgcc
     2341 cgaaagctcg agctgcccgg ccggctccag gctgacattg ccgtccttgc ccgacaaggc
     2401 gatgaccttg ccgccttcct cgaccctctc ccagccgaac tcggtcagag catcgaggag
     2461 gtcgcgaatc ccccccggct cgtcgtagga cggggctcgg tggtcggagc ggcggtagac
     2521 gaacttctca tgctcggtgc cgatccgcca ggcgctctgc ggcttttcgc ctttggcgaa
     2581 cggggccagc aggtcgtcgc gactttcgat cggcgggtcc gcgcgcgcgg tttcggtccg
     2641 ggtcgtcatc cccgccctct agctccggtc gcgaaaaccc gccagcctct tcccgttgcc
     2701 gaacgaaacc ggaatggatc aatgtcgtca ttccgggtca cgccctggaa gacgcggtcc
     2761 agtcgccggc agcgcccatc cacaaagtca cggccgcgag agccgcggtg tcggcgcgca
     2821 ggatgcgagg gccgaggctg atcggccggg cctgcggcaa agcgcggatc gccgcccgtt
     2881 cttcctcggt gaagccgcct tcggggccga tcaggatggc ggccgggccc ggctccgcgg
     2941 tgaacggctc gccccccgct tcgtcggcga agtgcaggat ccgctccccc ggccagccgg
     3001 cgagcagggc gccgagcttt tgcggctccg ccagctcggc gagcgaggtg cgttcgcact
     3061 gctcggcggc ctcgacgatg tgggcccgca gccgatcgag gttgagccgc tcgacgacgg
     3121 tccggcgggt gaggaccggg accagccggg cgactccgag ctcggtcgcc ttctcgacca
     3181 gccagtcgat ccggccgcgc ttgatcgggg cgaagagcag ccagaggtcc gggaccggct
     3241 cgcgggcgcg caggtgccgg gtaacggcga gtgtgacccg cttcttcccg gcctcggcga
     3301 cctcggcgag ccattctccg gtgcggtcgt cgaacagctt cacgccggcg cccgggccga
     3361 gccgaaggac gtt
//
LOCUS       DASWNU010000371         8772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_223102, whole genome shotgun sequence.
ACCESSION   DASWNU010000371 DASWNU010000000
VERSION     DASWNU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223102"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VGB57_07425"
     CDS             <1..616
                     /locus_tag="VGB57_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0131219.1"
                     /translation="VNRVALKDAEDNGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLP
                     LIEGTTVATKYGPMKTDHVLFIASGAFHIAKPSDLLPELQGRLPIRVELKALTEEDFV
                     RILSDTRASLTEQYIALIATEGVEVEFTDDGIRAIARIAAEVNGQVENIGARRLSTVL
                     EKLLEDVSFEAEERRGDRVVVDGPYVEAQLAEIARDTDLSKYVL"
     gene            complement(753..968)
                     /locus_tag="VGB57_07430"
     CDS             complement(753..968)
                     /locus_tag="VGB57_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131220.1"
                     /translation="MSNKHPSPDEQPVELRDDLDRDPGIGQSKAFFARDGKGGGHLVK
                     GDNTVEGDIENDGGDAGGVDPELGRDH"
     gene            1139..1450
                     /locus_tag="VGB57_07435"
     CDS             1139..1450
                     /locus_tag="VGB57_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY0131221.1"
                     /translation="MRKFTLAICAATMAVPVVPVLPTTAAFANHNGYYHGKTWRDSQG
                     RTRCRRANGTVGIIVGGAAGALVGRAIDGGRNRTTGTLLGAAAGALAGREVARSKRRC
                     R"
     gene            1662..2615
                     /locus_tag="VGB57_07440"
     CDS             1662..2615
                     /locus_tag="VGB57_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131222.1"
                     /translation="MSDRYDPMEEAFNDALEEAASGNGYEPRGFAADGFGNDGFGNDG
                     FGNDGFELEGFGPDGFGPEGFGDLGEYENEGDAFFGKVFKKFGGLVRQLPLKQLAQVG
                     ARVAGGALGGPVGAHLGGMAAGMLREDESELEGEGFDFEDESEGPLQEDEAEAFGLDS
                     FTDSLAESLATQAAESESEAEASALLGGVTIHIVSGAPVSVRRVSPALLRGANRLGRF
                     LRKRRRTRPLVKAIPKIQKTAVKMLADHARKGGKVTPATAAKAMARATVKTLGTPKAA
                     AKSLANNAVKSKRVGRAGTGAGTGAGAAASRPRSAIARAER"
     gene            2655..3560
                     /locus_tag="VGB57_07445"
     CDS             2655..3560
                     /locus_tag="VGB57_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131223.1"
                     /translation="MTYGSNGFGDPELEWESFGDLENEDESFNDLFGASDGEGINPFA
                     GGGALARMDTETAQMMMDVVAELASEAESEEEADAFLPIIAKLAPMALKAMAPLAKKA
                     FAKIAPKVSQSVFSAGRKMLQNFGQKGMAALPDIARGVARDSVQAVADGRDVTGDMVI
                     RSAAQHTLPFLQDPRQAQKALQRRRRPPQPRRPRVPPGYGRQQHQPYPSQPYDQPSYG
                     GETYGQSPYGPPAYAPPYGQSPYGQSPYGQSPHGQSPYGQSPYGQSPYGQSPYGQPAF
                     GQPDYPNGQSGDGQMPQPHYGQWGG"
     gene            3592..4929
                     /locus_tag="VGB57_07450"
     CDS             3592..4929
                     /locus_tag="VGB57_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131224.1"
                     /translation="MASTMVAIRAEGRPAPVPGGPGPAKARGSDPVSGFLRGQAVANA
                     GAALLLQPFAEGEFGRGAEGPSKAHLAEVNAMLSRMQRLIVENGGRMQQAIGAYAERP
                     GRKPIDEALRLKQEGGALISSIEHIRQFYWEIFGQRQTRIAPQLLAADRIALDCYQYV
                     YGGLGRARSIPSPAPLSYMESGTGPATFRRGVAMRKLLSLPNPFPLVKLPYSRLVTPW
                     TLGAIPHEIGHNLQSDLSLWLIVPKLINARLAAAGIPAADRGIWVRWTKETFADLIGV
                     LLIGPAFVESTMDVVGKAADSTGAFNPRGVHPTPYLRVRIGTHLLGKMGFTREARRID
                     EVWGRLYPDSVGDSIPASFRKNFPRSLPVVVDAIVGTPFTQLGGKTLRQVVCFRPQDQ
                     EVAVEAATRLAAGRDPGIVPERFLINAARIAFDRGLAAPGVIARHFYDALGRR"
     gene            4932..6089
                     /locus_tag="VGB57_07455"
     CDS             4932..6089
                     /locus_tag="VGB57_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131225.1"
                     /translation="MPDFNPSPAGGPDGALAWYNHSLAKVLGLNPADPTLPADLLSEL
                     NTAAQRKALAAVIDRRFKRHVDEVTGAVTMERVGDPLILIEPDEDSDPVIGGLATTYL
                     GAAQAAIAGIDPAQCIPGACAEQVAGLIRQIQANIATVTAEAPGRRNRYQMLLHLHEL
                     TDPNDGLLLRLGQVFGEGRNVPVDSVGWERAIGAIGLAKGALASFEMAIVELRAGDDE
                     PSLSEIAEIVGGCGAHVSTHARNVRTALRGAGIGGCELRGVELTIPTVQLSCEAFEMV
                     TLDQALQVLETEPARWAALIASGRRSGFDNVKRSAEAMLPMVNAINPTSILKTLGILA
                     ESEAQSEPREAFALALSYQLDRLRGYALSVLRMILREAPLQAPAAQANDHK"
     gene            6111..7235
                     /locus_tag="VGB57_07460"
     CDS             6111..7235
                     /locus_tag="VGB57_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131226.1"
                     /translation="MATREQYFTLLGQAAEMRSQMASLFANQRPHDVHARDILSNQLT
                     DLERQLGDMLGESTPVPPDKGLSALIGIGAEAGTGFGSVPAPIDVPSYDEAVFSERLF
                     AVGDLYYCYQLERLGIFRAVQKLQELFRAGKLRLGSGQGALELYRFDRKEVLRYTSKE
                     RMQAYRRVLGYGDAGPPPGARPNQAFHSLFSQFNLRVAQLFRDKRIAETFRPGAGASD
                     PGFGSIAMARRAGLDLRSNLKRASYGDVNVLTMELLQVVSSAFDILSAADIRHQFGTD
                     TGWDTLEEVLERYLGEAPVVSQRSRMAETGRAIILWLAQNFILTQGRTAFEALIQGIA
                     EMCEEWLTSAEALGAFKDQARTSGVLGNVVEFPSRRRSTA"
     gene            7361..7663
                     /locus_tag="VGB57_07465"
     CDS             7361..7663
                     /locus_tag="VGB57_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEY0131227.1"
                     /translation="MNLRSPRHRLFALERGDLDNSLDTSLSPREQEIARLVAIGYPNK
                     TIAGILDISPWTVATHVRRIFNKLNVHGRPAMIAELARAGFRFERRLSPARLPRPG"
     gene            complement(7672..>8772)
                     /locus_tag="VGB57_07470"
     CDS             complement(7672..>8772)
                     /locus_tag="VGB57_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY0131228.1"
                     /translation="SKEVGTLIQAMGTGIGRDDFNIEKLRYHKIVIMTDADVDGAHIR
                     TLLLTFFYRQMPEIIERGHLFIAQPPLYKVAKGRSEVYLKDNAKLDDYLVDAGIGLMR
                     LETEQGPRTGADLKDLADHARRVRSLMAYVPRRYDPGLIEALALNGALDPALPPAERS
                     QAIARAAAFLQASDESSTWTGEIGADGDYVLRRLWRGVTDHHVVDHKFVASAEARKLH
                     GLTAEQAATYASASRLVSMKTASAAAADEEEANLDSDDPHVKVEGVPDPGRAPDARGI
                     LVRRPTELLDAILAAGRKGLAIQRYKGLGEMNADQLWETTLDPDNRSLLRVEVAQADV
                     ADEIFTRLMGDVVEPRREFIQENALNVAKLDV"
BASE COUNT         1423 a         3066 c         2917 g         1366 t
ORIGIN      
        1 tgtcaaccgc gtcgccctga aggatgctga agacaacggc atcgtcttcc tcgacgagat
       61 cgacaagatc gcggtcagcg acgtgcgcgg cggctcggtc agccgcgaag gcgtgcagcg
      121 cgacctcctg cccttaatcg agggcaccac cgtcgccacc aaatatggtc cgatgaagac
      181 cgaccacgtc ctcttcatcg ccagcggcgc cttccatatc gccaagccgt cggacctgct
      241 gcccgagctc cagggccgcc tgccgatccg ggtcgagctg aaggcactga ccgaggagga
      301 cttcgtccgc atcctctccg acacccgcgc cagcctcacc gagcaatata tcgccctcat
      361 cgccaccgaa ggggtcgagg tcgagttcac cgacgacggc atccgcgcca tcgcccggat
      421 cgcggcggaa gtgaacggcc aggtcgagaa tatcggcgcc cgccgcctgt cgacggtgct
      481 cgagaaactg ctcgaggacg tcagcttcga agccgaggaa cgccgcggcg accgggtcgt
      541 ggtcgacgga ccctatgtgg aagcccagct ggccgagatc gcgcgcgata cggatctcag
      601 caaatacgtc ctctagcttg cctccccatg cgcagcatgg ggagggggac catccgcagg
      661 gtggtggacg gtagctggca gaagtctgcg ccaattaccc ctccaccatg cttcgcctgg
      721 tccccctccc catcttcgat ggggaggcaa aatcagtgat cgcggcccag ctccggatcg
      781 actccgccgg cgtcgccgcc gtcattctcg atatcgccct ccacggtgtt gtcccccttg
      841 accagatgcc cgccgccctt gccgtcgcgg gcgaagaagg ccttggactg gccgatgccg
      901 gggtcgcggt cgagatcgtc ccggagctcg accggctgct cgtcgggact cgggtgcttg
      961 ttgctcattc actgtctcct ctcgaccctc caacgcagca gaacggcgga cggttctgcg
     1021 cgcgcgatca agcgagcgga aatggcgctc atcggggatc gttgtcttag gttaatgaaa
     1081 ccgccaccac cctcgcccgt tcttggcacg agctttcacg gggaaaggaa cccaagtcat
     1141 gcgtaaattc accctcgcca tctgcgccgc caccatggcg gtcccggtcg tcccggtcct
     1201 cccgaccacc gcggccttcg ccaatcataa cggctattat cacggcaaga cctggcgcga
     1261 ctcgcagggc cgcacccgct gccgccgggc caacggcacg gtcgggatca tcgtcggcgg
     1321 cgccgccggc gctctcgtcg gccgggcgat cgacggcggc cgcaaccgca ccacgggtac
     1381 cctcctcggc gccgccgcgg gcgctctggc cggccgcgag gtcgcccgca gcaagcgccg
     1441 ctgccgctga tttggcaacc tgactgattg aggcggggcg tccttcgggg cgccccgttt
     1501 cgattccggc tccgcgagtc ggcggccggc cgacaggcgg tcaagtcctc ccgacgagct
     1561 accccggacg atccaccgcg ctttggcgac atgcgcggcc ccgcggctcc cccctacaag
     1621 ggcctctcgg cttcggccgc aggtcagatg ggagcaatgc gatgagcgac cgctacgatc
     1681 cgatggagga agccttcaac gacgcgctcg aggaagccgc gtcggggaac ggctacgagc
     1741 cgcggggctt cgccgccgac ggcttcggca atgacggctt cggcaatgac ggcttcggca
     1801 acgacgggtt cgagctggag ggtttcggcc cggacggctt cggccccgaa gggttcggcg
     1861 acctcggcga atatgagaat gagggcgacg ccttcttcgg caaggtcttc aagaaattcg
     1921 gagggctggt ccgtcagctc ccgctcaagc agctcgcgca ggtcggagcc cgggtcgcgg
     1981 gcggagcgct cggcggaccg gtcggagccc atctcggcgg aatggccgcc ggaatgcttc
     2041 gcgaggacga atccgagctc gaaggcgaag ggttcgactt cgaggacgag agcgaaggtc
     2101 cgctgcagga agatgaagcc gaggcgttcg gcctcgactc gttcaccgac agcctcgccg
     2161 agtccctcgc cacccaggcg gccgaaagcg agagcgaggc cgaggcgtcg gccctgctcg
     2221 ggggagtgac catccacatc gtcagcggcg ctccggtttc ggtccgcagg gtctcgccgg
     2281 cgctcctgag gggcgccaac cggctcggcc gcttcctccg caagcgccgc cgcacccggc
     2341 cgctggtgaa ggcgataccc aaaatccaga agaccgcggt caagatgctc gccgaccatg
     2401 cccgcaaggg cgggaaggtg actccggcga cggccgccaa ggccatggcc agggccacgg
     2461 tcaagaccct cgggacgccc aaggccgccg cgaagagcct cgccaacaac gcggtgaaga
     2521 gcaagcgggt cggccgcgcc ggcaccggcg ccggcaccgg cgccggcgcc gccgcctccc
     2581 gcccgcgcag cgcgatcgcc cgggccgagc gctgagctcc accccgcttg tcatccaggc
     2641 agaaaggtac ggccatgacc tacggcagca acggcttcgg cgatcccgag ctcgaatggg
     2701 aatcgttcgg agacctcgag aacgaggacg aatcgttcaa cgacctgttc ggggcgtcgg
     2761 acggggaggg catcaacccg ttcgccggcg gcggcgccct cgcccggatg gacaccgaga
     2821 ccgcgcagat gatgatggac gtcgtggccg agctcgcctc ggaggcggag agcgaggagg
     2881 aggcggacgc cttccttccg atcatcgcca agctggcgcc gatggcgctc aaggccatgg
     2941 cgccgctggc caagaaggcg ttcgccaaga tcgccccgaa ggtctcgcaa agcgtgttca
     3001 gcgccggccg caagatgctt cagaatttcg gccagaaggg catggcggcc ttgccggaca
     3061 tcgcccgcgg agtcgcccgc gacagcgtcc aggcggtcgc cgacggccgc gacgtcaccg
     3121 gcgacatggt gatccgctcg gccgctcagc ataccctgcc cttcctccag gatccgcgcc
     3181 aggcgcaaaa ggcgttgcag cggcggcggc ggcctccaca gccgcggcgt ccccgagtcc
     3241 cgccaggcta cggaaggcag cagcaccagc cctatccgtc tcaaccgtac gaccagccgt
     3301 cctacggcgg ggaaacgtac gggcagtcgc cttacggccc cccggcctac gcgccgcctt
     3361 acgggcaatc gccctatggt cagtcgcctt acggccagtc gcctcacggc cagtcgcctt
     3421 acggccagtc gccttacggg caatcgcctt acgggcaatc gccttatggc cagccggcct
     3481 tcggccagcc cgattatccg aacggccagt ccggcgacgg gcagatgccc cagccgcatt
     3541 acggccagtg gggcggctga gcgcccggcg ctcgggacag ggagaccggc catggcttcg
     3601 acgatggtag cgatccgggc ggaagggcgc ccagcccccg tgcccggcgg cccgggcccg
     3661 gcgaaggcga ggggcagcga tcccgtctcg gggttcctgc gcggccaggc ggtcgccaac
     3721 gccggcgccg cgctgcttct ccagccgttc gccgaaggcg agttcggccg gggcgccgag
     3781 ggcccgtcaa aggcccacct cgccgaagtt aacgcgatgc tcagccggat gcagcgcctc
     3841 atcgtcgaga atggcggccg gatgcagcag gcgatcgggg cctatgccga gcggccgggc
     3901 cgcaaaccga tcgacgaggc gcttcggctg aagcaggaag gcggcgccct gatctcgtcg
     3961 atcgagcata taaggcaatt ctactgggag atattcggcc agcggcagac ccggatcgcg
     4021 ccgcagctcc tcgccgccga ccggatcgcg ctcgattgct atcaatatgt ctatggcggc
     4081 ctcggccggg cccgctcgat cccctcgccg gcgccgcttt cctacatgga atccgggacc
     4141 ggaccggcga ccttccggcg cggcgtcgcc atgcgcaagc tgctcagcct tcccaacccc
     4201 ttcccgctgg tgaagctgcc ctacagccgc ctggtgaccc cctggaccct gggcgcgatc
     4261 ccgcacgaga tcggccacaa tctccagtcc gacctcagcc tgtggctgat cgtgcccaag
     4321 ctgatcaacg cccggctggc ggcggccggg atccccgccg ccgaccgcgg catctgggtg
     4381 cggtggacca aggagacgtt cgccgacttg atcggagtgc tcctgatcgg cccggccttc
     4441 gtcgaatcga ccatggacgt ggtcggcaag gcggccgatt cgaccggcgc cttcaacccg
     4501 cggggagtcc atcccacgcc ttacctgcgg gtgcgaatcg gcacccattt gctgggcaag
     4561 atgggcttca ccagggaggc ccggcgcatc gacgaggtct ggggccggct ctatcccgac
     4621 agcgtcggcg attcgatccc ggcctcgttc cgcaagaatt tcccgcgctc gctgccggtg
     4681 gtcgtcgacg cgatcgtcgg cacgccgttc acccagctcg gcggcaagac cctgcgccag
     4741 gtcgtctgct tccgacccca ggaccaggag gtggcggtcg aagccgcgac ccggctcgcc
     4801 gcgggccgag accccgggat cgtcccggag cggttcctga tcaatgccgc ccgcatcgcc
     4861 ttcgaccgcg gcctcgccgc gccgggagtg atcgcccgcc atttctacga cgcgcttgga
     4921 aggagatgag catgccggat ttcaatccct cgcccgccgg cggtcctgac ggcgccctcg
     4981 cctggtacaa tcacagcctg gccaaggtgc tcggcctcaa cccggccgac ccgaccctgc
     5041 ccgccgacct gctcagcgag ctgaatacgg ccgctcagcg caaggcgctc gccgcggtga
     5101 tcgaccggcg cttcaagcgc cacgtcgacg aagtcaccgg cgccgtcacg atggagaggg
     5161 tgggcgatcc gctcatcctc atcgagccgg acgaagattc cgatcccgtc atcggcggcc
     5221 tcgcgacgac ctatctgggc gccgcccagg cggcgatcgc cggcatcgac ccggcccagt
     5281 gcattccagg cgcctgcgcg gagcaggtgg ccggactgat ccggcagatc caggccaaca
     5341 tcgccaccgt caccgccgag gcgccgggac ggcgcaaccg ctatcagatg ctcctccatc
     5401 tgcatgagct caccgatccg aacgacggac ttctgctcag gctcggccag gtgttcggcg
     5461 agggccggaa cgtgccggtc gactcggtgg gctgggagcg ggcgatcggc gccatcggcc
     5521 tggccaaggg cgcgctcgcc agcttcgaga tggcgatcgt cgagcttcgc gccggggacg
     5581 acgagccgag cctctccgag atcgccgaga tcgtcggcgg ctgcggcgcc catgtctcga
     5641 cccatgcgcg caacgtgcgc acggcgttgc gcggcgcggg catcggcggg tgcgagctgc
     5701 gcggggtgga gctgacgatc ccgaccgtcc agctcagctg cgaagcgttc gaaatggtca
     5761 ctctcgacca ggcgcttcag gtgctcgaga ccgaaccggc ccgctgggcc gccctgatcg
     5821 cgagcggccg gcgctccggc ttcgacaacg tcaagcgttc ggccgaggcg atgctgccga
     5881 tggtcaacgc gatcaatccg acctcgatcc tgaagaccct cggaatcctc gccgaatccg
     5941 aggctcagtc ggagccgcgc gaggccttcg ctttggccct gtcctaccag ctggaccggc
     6001 tgcgcggcta cgctttgtcc gtcctcagga tgatcctgcg cgaagcgccg ctccaggcgc
     6061 cggccgcaca ggcgaacgat cacaaatagg ctcttaccgg gagacgagac atggctaccc
     6121 gcgaacaata tttcaccttg ctggggcagg cggcggagat gcgctcgcag atggcgtcgc
     6181 tgttcgccaa ccagcgcccg cacgacgtcc atgcgcgcga catcctctcc aaccagctga
     6241 ccgatctcga gcgccagctc ggcgacatgc tgggggaatc gactcccgtc ccgcccgaca
     6301 agggcctcag cgcgctgatc gggatcggcg cggaggcggg taccggcttc ggaagcgtgc
     6361 ccgcgccgat cgacgttccg tcctacgacg aggcggtgtt ctcggagcga ctgttcgccg
     6421 tcggcgacct ctattattgc taccagctcg aacgcctggg cattttccgc gcggtgcaga
     6481 agctccagga attgttccgc gcgggcaagc tcaggctcgg ctccggccag ggcgcgctcg
     6541 agctctaccg cttcgaccga aaggaggtgc tccgctacac cagcaaggag cgcatgcagg
     6601 cctatcgccg ggtgctcggc tacggcgacg cggggccgcc ccccggggcc cgtccgaacc
     6661 aggccttcca cagcctgttc agccagttca acctgcgagt cgcccagctc ttccgcgaca
     6721 agaggatcgc cgagaccttc cggccgggcg ccggcgcttc cgacccgggg ttcggaagca
     6781 tcgccatggc ccggcgggcc gggctcgacc tcaggagcaa tctgaagagg gcctcctacg
     6841 gcgacgtcaa cgtgctcacc atggagcttc ttcaggtggt ctcgtccgcc ttcgacatcc
     6901 tgagcgccgc cgacatccgc caccagttcg ggaccgacac cggctgggac actctcgaag
     6961 aggtgctcga gcgctatctc ggcgaagctc cggtcgtttc gcaacggtcg cgtatggccg
     7021 agacagggcg ggcgataatt ctctggctcg cccagaactt catcctgact caaggccgca
     7081 ccgcctttga ggcgctcatc caaggtatcg ccgagatgtg cgaggaatgg ctgaccagcg
     7141 ccgaggctct cggcgctttc aaggatcagg ctcgtacttc cggcgtcctt ggcaacgtcg
     7201 tcgaatttcc ttcgcggcgc cgctcgaccg cctgaaagcg ctggcaggaa ccccccgcct
     7261 gcgtataaag cattccgggg gcagaaaaag aagttgggcg gtgggccgaa gatactcttc
     7321 gggacggcca atcttctttt gctcgtccgt gggggtgcca atgaaccttc gttcgcctcg
     7381 tcaccgcctc ttcgcgctgg agcgcggtga tctcgacaat tctctcgaca cgtcgctcag
     7441 cccccgggaa caggaaatcg cccggctggt cgcgatcggc tatcccaaca agaccatagc
     7501 cggaattctc gacatcagcc cctggaccgt cgccacgcat gtccggcgca tcttcaacaa
     7561 gctcaacgtc cacggccggc cggcgatgat cgccgagctg gcccgggccg gcttccgctt
     7621 cgaacgacgg ctgagtccgg cccggctgcc gcggccgggc taggtcccaa atcagacgtc
     7681 gagtttggcg acgttgagcg cgttctcctg gatgaactcg cgacgcggct cgacgacgtc
     7741 gcccatcagg cgggtgaata tctcgtcggc gacgtcggcc tgggcgacct cgacgcggag
     7801 cagcgagcgg ttgtccgggt cgagcgtggt ttcccagagc tggtcggcgt tcatctcgcc
     7861 caagcccttg tagcgctgga tggcgaggcc cttgcggccg gcggcgagga tcgcgtcgag
     7921 caattcggtc ggccggcgga cgagaatccc gcgggcgtcg ggcgcccggc cgggatcggg
     7981 gactccttcg accttgacgt gcgggtcgtc gctgtcgaga ttggcctcct cttcgtccgc
     8041 ggcagcggcc gaggcggtct tcatcgagac caggcgggag gcgctggcgt aggtggcggc
     8101 ctgctcggcg gtgaggccgt gcagcttgcg cgcctcggcc gacgcgacga acttgtggtc
     8161 gacgacatga tggtcggtca ctccgcgcca gaggcggcga aggacatagt cgccgtcggc
     8221 gccgatctcg ccggtccagg tcgaggattc gtcgctggcc tgaaggaagg cggcggcgcg
     8281 ggcgatcgct tgcgagcgct cggccggcgg cagcgccgga tcgagggcac cgttcagagc
     8341 cagggcctcg atcaggccgg gatcgtatct gcgcgggaca taggccatca ggcttcgcac
     8401 ccggcgggca tggtcggcga ggtccttgag gtcggcgccg gtgcgcggcc cctgctccgt
     8461 ctcgagccgc atcaggccga tgcccgcgtc gaccagatag tcgtcgagct tggcattgtc
     8521 cttgagatag acttcggacc gccccttggc gaccttgtag agcggcggct gggcgatgaa
     8581 gaggtgcccg cgttcgatga tctcgggcat ctgccggtag aagaaggtca gcaggagcgt
     8641 gcggatatgg gcgccgtcga catcggcgtc cgtcatgatc acgatcttgt ggtagcgcag
     8701 tttctcgatg ttgaaatcgt cgcggccgat gccggtgccc atcgcctgga tgagggttcc
     8761 gacctccttg ga
//
LOCUS       DASWNU010000372         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_402617, whole genome shotgun sequence.
ACCESSION   DASWNU010000372 DASWNU010000000
VERSION     DASWNU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402617"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..626
                     /locus_tag="VGB57_07475"
     CDS             66..626
                     /locus_tag="VGB57_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY0131229.1"
                     /translation="MGKVINFEDRAVAKLRARVAAAEEANQDLIAFARGHSGAVASIH
                     EAVLAAIEADGFDHLIHIVTQEWPQILGLDAVAVALFAGDKGVRADASGLQFVDARVI
                     RRSLEGLDGVVLRGVGRGHPLFGPACELIRAEALVRLDSEPPLPSGLLALGQREAQGF
                     ETRHGSELLMFLGRVLTRSMGRWLIP"
     gene            611..1540
                     /locus_tag="VGB57_07480"
     CDS             611..1540
                     /locus_tag="VGB57_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY0131230.1"
                     /translation="MADPLSGHSASGTALRWQQHLAHDRRRSPHTVRAYAATAHRLIG
                     FLAGHLGGAVDGPALAGLEAKDLRAFLAHRRSEGLGNSSAARELSAVRSFLAFASAER
                     GGEPGLPRLKGPKRPRSVPRPVAPNDVLALAEEAGEEASEPWIAARDRAVLLLLYGSG
                     LRVAEALGLTGAALPLGEALSVTGKRAKTRIVPLLAPVRAAIEDYLSICPYAPSSGEP
                     LFRGARGGALNGEMIRRVVRRARARLGLSDRTTPHALRHSFATHLLGRGADLRSLQEL
                     LGHASLSSTQIYTAVDAAHLLDVYRNAHPRAQP"
     gene            complement(1668..2270)
                     /locus_tag="VGB57_07485"
     CDS             complement(1668..2270)
                     /locus_tag="VGB57_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0131231.1"
                     /translation="MGDWTIRLIDQTGYVGIFLLMFLETIFPPIPSEVIMPIAGARAA
                     QGPLTLWGVIASGTAGAMLGNLFWYALARVIGMKRFRPFIEKHGRWLTMDWDDVEKAE
                     KLFGRFGGTIVSLGRMLPTIRSVISIPAGLLSMRLKTFLIWSTLGTVAWTAILATAGW
                     GLGMAFEDVEKILGPLSTAIIALIVVAYVWRQITWRKRHP"
BASE COUNT          335 a          852 c          747 g          373 t
ORIGIN      
        1 ctcgtctccc gcgggctcga atttcgccat cgcttcttaa ccttgttcgg gttagcttcg
       61 gaccgatggg aaaggtgatc aacttcgagg atcgggcggt ggccaagctt cgcgcccggg
      121 tcgcggcggc ggaggaggcc aatcaggacc tcatcgcctt cgcccgcggc cattcgggcg
      181 ccgtcgcctc gatccacgag gcggtgctcg cggcgatcga ggccgacggg ttcgaccatc
      241 tcatccacat cgtcactcag gaatggcccc agatactcgg cctcgacgcc gtcgccgtcg
      301 ccctgttcgc cggcgacaag ggcgtccgcg ccgacgcctc cggacttcag ttcgtcgacg
      361 cccgggtcat ccgccgctcg ctcgaggggc tcgacggggt cgtccttcgc ggagtcggcc
      421 ggggccatcc cctgttcggg ccggcctgcg agctgatccg ggccgaggcc ctcgtccgcc
      481 tcgacagcga gccgcctctg ccgagcggcc tgctcgccct cggccagcgc gaggcgcagg
      541 ggttcgagac gcgccacggc tccgaattgc tcatgttcct cggccgcgtc ctgacccgct
      601 cgatgggccg gtggctgatc ccctgagcgg ccactctgcg tccggcacgg cgctgcgctg
      661 gcagcagcat ctcgcccacg accgccgccg ctctccccat accgtccgcg cttatgccgc
      721 caccgcccat cgcctgatcg gctttctcgc cggccatctc ggcggcgcgg tcgacgggcc
      781 cgccctcgcc ggcctcgagg cgaaggatct gcgcgccttc ctcgcccaca ggcggagcga
      841 agggcttggc aacagctcgg cggcgcgcga gctctccgcc gtccgttcct tcctcgcctt
      901 cgcctcggcc gagcggggcg gggagccggg tctgccccga ttgaagggcc cgaagcggcc
      961 gcgcagcgtt ccgaggccgg tcgcgcccaa cgacgtcctc gccctcgccg aggaagccgg
     1021 cgaggaggcc agcgagccct ggatcgcggc ccgcgaccgg gcggtgctcc tgctcctcta
     1081 cggctccggc cttcgggtcg ccgaggcgct cggcctgacc ggcgcggcac tgccgctcgg
     1141 ggaggcgctg agcgtcaccg gcaagagggc gaagacccgg atcgtgcccc tgctcgcgcc
     1201 ggtccgggcc gcgatcgagg attatctctc gatctgccct tatgccccgt cgagcggcga
     1261 gcccctcttc cgaggcgctc gcggcggggc cctgaacggc gagatgatcc gccgggtcgt
     1321 ccgccgcgcc cgcgcccgcc tcggcctctc ggaccggacc accccccacg cccttcgcca
     1381 cagcttcgcg acccacctgc tcggccgggg cgccgacctg cgctcgctcc aggagctgct
     1441 cggccacgcc agcctctcct cgacccagat ctacacggcc gtcgacgccg cccacctcct
     1501 cgacgtctac cgcaacgccc atccgagggc gcagccgtaa aagagggaca gtccctcttt
     1561 tacggacgat ggaaaagagg gactgtccct ctttacgctg acgatgatgg aaaagaggga
     1621 ctgtccctct ttacgctgga ttgcttcgct gcgctcgcaa tgacgggtta cggatgccgt
     1681 ttgcgccagg tgatctggcg ccagacgtag gcgacgacga tgagggcgat gatcgctgtc
     1741 gacagcgggc ccaaaatttt ctcgacgtct tcgaaggcca ttcccaggcc ccagcccgcg
     1801 gtcgccagga tcgccgtcca cgcgaccgtg ccgagggtcg accagatcag gaacgtcttg
     1861 aggcgcatgc tgagcaggcc cgccgggatc gagatgaccg agcggatggt gggaagcatc
     1921 cggcccaacg agacgatcgt tcctccgaag cggccgaaca gcttttcggc cttctcgaca
     1981 tcgtcccaat ccatcgtcag ccagcggccg tgcttctcga tgaagggacg gaagcgcttc
     2041 attccgatca cccgggcgag cgcgtaccag aagaggttgc cgagcatcgc gccggcggtg
     2101 ccgctcgcga tcacccccca caaggtgagc ggcccctgag ccgcccgtgc cccggcgatg
     2161 ggcatgatca cttccgaggg gatgggcggg aagatcgtct ccaggaacat cagcaggaag
     2221 atgccgacat agccggtctg gtcgatcagt cggatggtcc agtcgcccat ctcagcgaat
     2281 cttcgcttcg atcgcgtccc agatcat
//
LOCUS       DASWNU010000373         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_247845, whole genome shotgun sequence.
ACCESSION   DASWNU010000373 DASWNU010000000
VERSION     DASWNU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247845"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGB57_07490"
     CDS             complement(<1..477)
                     /locus_tag="VGB57_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0131232.1"
                     /translation="MKLTILPNGLKVASREMPALETAAVGLYAEAGSRHEPAPLNGIA
                     HLFEHMVFKGAGGRSAREISEAIEDVGGDLNASTERDATSFTASVLAEHVPLGVELIT
                     DLILNPHFVEADLEREKDVVLQELGEARDTPNDNIFDDLWSAAFAGQALGRSVLGEE"
     gene            596..1021
                     /locus_tag="VGB57_07495"
     CDS             596..1021
                     /locus_tag="VGB57_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131233.1"
                     /translation="MRRSGKAGVWAVAIGLLGIGAIPASAMWLQGPWVNGRPDIDTGD
                     MLFEGCMRPGVESSCKVIDASSQRTDGQGRPVGEPVISTYAVVAASPMPAYDRRLVVL
                     ARPSKGRARICGGEAVEIKNWYYKAGPPCPERDWSPFDN"
     gene            complement(967..1629)
                     /locus_tag="VGB57_07500"
     CDS             complement(967..1629)
                     /locus_tag="VGB57_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEY0131234.1"
                     /translation="MRFPLLPTILVGAAVATMIALGFWQLRRADEKTALQRQYERNAG
                     LPAMALPPAAVADETLLYRRATAFCLEVTAWRTTGGKAASGRGGTRFIAECRTGAEGP
                     GFVADMGVSPDPRAKPAWKGGEVAGRIVAEPRSGGLFERLTGKSPPPRPMIVSEAPAP
                     GLQASAPPTAEAPNNSLFYAFQWFFFAAAAAVIYGLALRRRRAQLSNGDQSRSGQGGP
                     AL"
     gene            complement(1629..1988)
                     /locus_tag="VGB57_07505"
     CDS             complement(1629..1988)
                     /locus_tag="VGB57_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY0131235.1"
                     /translation="MKEPTPSAVALRGLCPRCAAKSLYKGLAGFAPRCRACGLDFAGF
                     NVGDGPAAFLILIVGAVVTGLAITLELAAEPPFWVHVLLWLPLTAALVVGSLRIAKGL
                     LLALEYRHRAREGRISG"
     gene            complement(2117..>2733)
                     /locus_tag="VGB57_07510"
     CDS             complement(2117..>2733)
                     /locus_tag="VGB57_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0131236.1"
                     /translation="HAGDHTPVVQLHLRYGMILFIASEVMFFLAWFWAYFNAALFPPG
                     VESVGGTWPPKGMEVLDPFGYPLLNTLILLCSGTTVTWAHHALIHGDRNGLIKGLWAT
                     IGLGLLFTSIQAYEYAHAPFGFTDNIYTSTFFMATGFHGFHVIVGTIFLIVCLVRAYK
                     GHFTPRQHFGFEAAAWYWHFVDVVWLFLFICIYVWGGWGAEYHG"
BASE COUNT          475 a          861 c          987 g          410 t
ORIGIN      
        1 ttcctcgccc agcactgagc ggccgagcgc ctgcccggcg aaggccgcgg accagagatc
       61 gtcgaagatg ttgtcgttgg gggtgtcgcg cgcctccccc agctcctgca ggacgacgtc
      121 cttctcgcgc tccaggtcgg cctcgacgaa gtggggattc aggatcaggt cggtgatcag
      181 ctcgacgccc aggggcacgt gctcggcgag gacggaggcc gtgaagctgg tggcgtcgcg
      241 ctcggtcgag gcgttgaggt cgccgccgac atcctcgatc gcctcgctga tctcgcgcgc
      301 cgagcgtccg ccggcaccct tgaacaccat atgctcgaac agatgggcga tcccgttgag
      361 gggcgccggc tcgtgccgcg accccgcttc ggcgtagagc ccgacggcgg ccgtctcgag
      421 ggcgggcatc tcgcggcttg cgaccttgag gccgttggga aggatggtga gcttcattgg
      481 acgcgctcat ctagggggtt agcgccgacg cggcaacaat cgggaacgat gtccaaacga
      541 gttgcggcgt tcggacggcg gacttaagct tctttttttg aaggggggct ggcgaatgag
      601 gcgatcaggc aaagccggcg tctgggccgt agcaatcggg ctgctcggca tcggcgccat
      661 tccggcatcg gccatgtggc ttcaggggcc gtgggtcaac ggccgtcccg atatcgacac
      721 cggcgatatg ttgttcgagg gctgcatgcg gccgggcgtc gaatcgtcct gcaaggtgat
      781 cgacgcctcg tcccagcgca cggacgggca gggccgtccg gtcggcgagc ccgtgataag
      841 cacctacgcg gtggtcgcgg cttcgccgat gccggcttac gacaggcgtt tggtcgttct
      901 cgcgcggccg tccaaggggc gcgcgcgcat ctgcggcggc gaggccgtcg agatcaagaa
      961 ctggtattac aaggccggac cgccctgtcc ggagcgggat tggtcgccgt tcgacaattg
     1021 agcgcggcgg cggcgcagag cgagcccgta aatgacggcc gccgcggccg cgaagaagaa
     1081 ccactggaag gcgtagaaca ggctgttgtt tggggcctcg gcggtcggcg gcgcgctcgc
     1141 ctggagaccc ggcgccggcg cttcggaaac gatcatcggc cgcggcggcg gcgacttgcc
     1201 ggtgagacgc tcgaacaggc ccccggatcg aggctcggcg acgatcctgc cggccacttc
     1261 gccgcccttc caggcgggct tcgccctggg gtcgggagag acgcccatgt ccgcgacgaa
     1321 gcctggacct tcggctccgg tgcggcactc ggcgatgaag cgggttccgc cccgtccgga
     1381 cgcggccttg ccgcccgtcg tccgccacgc cgtcacctcg aggcagaaag cggtggcgcg
     1441 ccggtagagc agggtctcgt ccgccaccgc ggcgggcggc agagccatgg ccggaaggcc
     1501 cgcgttgcgc tcatattggc gttgcagcgc cgtcttctcg tcggcgcggc gcagctgcca
     1561 gaagccgagc gcgatcatcg tcgccacggc ggcgccgacg aggatggtgg ggaggagcgg
     1621 aaagcgcatc agccggagat ccgcccttcg cgggcgcggt ggcgatattc cagggcgagc
     1681 aggaggccct tggcgatccg aagcgagccg accaccaaag ccgccgtcag cggcagccag
     1741 agcaggacat ggacccagaa aggcggctcg gcggccagct cgagcgtgat cgccaggccg
     1801 gtgacgaccg ctccgacgat caggatcagg aacgcggcgg gcccgtcgcc gacgttgaag
     1861 ccggcgaagt cgagcccgca ggcccggcat ctgggcgcga agcccgccag ccccttgtac
     1921 agcgacttcg cggcgcagcg cggacacagg ccgcgcaagg cgacggcgga gggggtgggc
     1981 tccttcaccc tttcgtcatc ccggcggaag ccgggacctc aggtcgtaga gtcacagacc
     2041 ttctccccga ggtaccgggt ttcgggaccc catcgaagta ccacttcgat ggggtccctc
     2101 ccggtatgac ggaaaatcac ccgtgatatt cggcgcccca gccgccccag acgtagatgc
     2161 agatgaacag gaacagccac acgacgtcga cgaaatgcca ataccaggcc gccgcctcga
     2221 agccgaaatg ctggcgcggc gtaaaatggc ctttgtaggc gcggaccagg cagacgatca
     2281 ggaagatggt cccgacgatg acgtggaagc cgtggaaccc ggtcgccatg aagaaggtcg
     2341 aggtgtagat attgtccgtg aagccgaacg gcgcatgggc gtattcatag gcctggatgc
     2401 tggtgaacag caggccgagg ccgatcgtcg cccagagccc tttgatgagt ccgttacggt
     2461 cgccgtggat gagggcgtga tgggcccagg tcacggtcgt tcccgagcag agcaggatga
     2521 gcgtgttgag cagcgggtag ccgaacggat cgaggacctc catccccttg ggcggccagg
     2581 tgccgccgac cgattcgacc cctggcggga agagggcggc gttgaaatag gcccagaacc
     2641 aggcgaggaa gaacatcacc tccgaggcga tgaacaggat catcccgtag cgaaggtgga
     2701 gctggacgac cggagtatgg tcgccggcat gcg
//
LOCUS       DASWNU010000374         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_143141, whole genome shotgun sequence.
ACCESSION   DASWNU010000374 DASWNU010000000
VERSION     DASWNU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143141"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VGB57_07515"
     CDS             complement(<1..199)
                     /locus_tag="VGB57_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY0131237.1"
                     /translation="MSLESVRAWLAGHAPDLPLIEVEESTATVETAARALGVEPGRIA
                     KTLAVRAGGTTFLLVARGDARL"
     gene            complement(204..623)
                     /locus_tag="VGB57_07520"
     CDS             complement(204..623)
                     /locus_tag="VGB57_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY0131238.1"
                     /translation="MRQATGTLEFHTPRQGLLEITGDVTRWVSDQAMTDGLLTLFCRH
                     TSASLIIQENAAPEVRSDIEAWIARLAPEDPSAYAHRDEGPDDMPAHLRTILTQVQLA
                     VPLTGGRLALGTWQGIYLFEHRRRPHRRSLALHLIGE"
     gene            883..2289
                     /gene="argH"
                     /locus_tag="VGB57_07525"
     CDS             883..2289
                     /gene="argH"
                     /locus_tag="VGB57_07525"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0131239.1"
                     /translation="MWGGRFSGGPAALMREINASIGFDKRLWREDIAASLAHVAMLET
                     QGIIPEGDASAIAEGLERIEDEYARIGVREDPALEDIHMHVEARLAELIGPSAGRLHT
                     ARSRNDQVATDFRLWVRGAVDEADAALAAFQRVLVARAEEHVETAMPGFTHLQSAQPV
                     TLGHHLMAYYEMLARDRSRFAAARERLNESPLGAAALAGTGFPIDRDATAAALGFDRP
                     SANSLDAVSDRDFALDYLMAATQCALHLSRLAEELIIWASQPFGFAILPDAFSTGSSI
                     MPQKRNPDAAELVRGHSGRIAGAMVALVMTMKGLPLAYSKDMQDDKPPVFEAHDLLLL
                     SLQALAGMVEATRFDEARMRSAAEAGFATATDLADWLVREAGVPFREAHHIVGRAVAA
                     AEERGVGLAELELEAMRGIDPRIDERVFGVLTVDASVRSRTSYGGTAPERVREQIARA
                     KRALDLPPHAQHGEGDPA"
     gene            complement(2413..>2649)
                     /locus_tag="VGB57_07530"
     CDS             complement(2413..>2649)
                     /locus_tag="VGB57_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131240.1"
                     /translation="LAAWGQRNNFSNAFPAHGRDQDALIGNRAVPGATKVFRIPPGPA
                     GGGTVTGLPDFVRTKATQYLLMPSGQTLSRLVPL"
BASE COUNT          373 a          965 c          924 g          387 t
ORIGIN      
        1 cgagcctggc gtcgccgcga gcgacgagca ggaacgtcgt gccgcccgcc cggaccgcca
       61 gagtcttggc gatccgcccc ggctcgaccc ccagcgcccg ggccgccgtc tcgaccgtgg
      121 ccgtgctctc ctcgacctcg atcagcggca gatcgggcgc gtggccggcg agccaggctc
      181 ggacggattc gaggctcacc tcctcactcg ccgatcaggt ggagcgcgag gcttcgccgg
      241 tgcggccggc ggcgatgctc gaacaggtag atgccctgcc aggtgccgag cgccagccgc
      301 ccgccggtca gcgggacggc gagctggacc tgggtcagga tcgtcctcag atgggcgggc
      361 atgtcgtcgg ggccttcgtc gcggtgcgcg taggcggagg ggtcctccgg ggccagccgc
      421 gcgatccacg cctcgatatc cgatcggacc tctggcgcgg cattttcctg gatgatgagc
      481 gaggcggagg tgtggcggca gaagagagtg agcaggccgt cggtcatcgc ctggtcggag
      541 acccagcggg tgacgtcgcc ggtgatttcg agcaggccct ggcggggcgt gtggaattcg
      601 agggtgccgg tggcctggcg catggcgggg gattagcgcc tctgcctccc catcgccaga
      661 tggggagggg gaccatgcga agcacggtgg aggggtagcc gccgatgaat tcgaaattct
      721 cgcgcgaacc gctcgactcg gcgccaatta cccctccacc accttcggtg gtccccctcc
      781 ccatctgacg atggggaggc aaatcttggc cccgcccccg ctttgcccta tcgcctcgcc
      841 cgaaggagaa aagccgtcgc cgaggacaaa cccagcaacc tgatgtgggg cggccgcttc
      901 tccggcggtc cggcggcgct gatgcgcgag atcaacgcct cgatcggctt cgacaagagg
      961 ctgtggcgcg aggacatcgc cgcctcgctc gcccacgtcg cgatgctcga gacccagggg
     1021 ataatcccgg aaggcgacgc ctcggccatc gccgaggggc tggagcggat cgaggacgaa
     1081 tatgcgcgga tcggagtccg cgaggatccg gccctcgagg acatccacat gcatgtcgag
     1141 gcccggctgg ccgagctgat cggcccgtcc gccggccgcc tccacaccgc ccgctcgcgc
     1201 aacgaccagg tcgccaccga cttccgcctc tgggtccggg gcgccgtcga cgaggccgac
     1261 gcggcgctcg ccgccttcca gcgcgtgctg gtcgcccggg cggaggagca tgtcgagacg
     1321 gcgatgcccg gcttcaccca tctccagtcg gcgcagccgg tgacgctcgg ccaccatctc
     1381 atggcctatt acgagatgct ggcgcgcgac cgctcccgct tcgccgccgc gcgcgagcgg
     1441 ctcaacgaga gcccgctcgg cgccgccgcc ctcgccggca ccggcttccc gatcgaccgc
     1501 gacgccaccg ccgccgccct gggcttcgac cgcccctcgg ccaactcgct cgacgcggtc
     1561 tccgaccgcg acttcgcgct cgattacctg atggccgcga cccaatgcgc gctccatctc
     1621 tcccgcctcg ccgaggagct gatcatctgg gcgagccagc ccttcggctt cgccatcctg
     1681 cccgacgcgt tcagcaccgg ctcctcgatc atgcctcaga agcgcaaccc cgacgccgcc
     1741 gagctggtcc gcggccatag cgggcggatc gccggcgcga tggtcgccct ggtcatgacc
     1801 atgaagggcc tgccgctcgc ttattcgaag gacatgcagg acgacaagcc gcccgtcttc
     1861 gaggcccacg acctcctcct cctgtcgctc caggcgctcg ccgggatggt ggaggcgacc
     1921 cgcttcgacg aggcccgcat gcgctccgcg gcggaggccg gcttcgccac cgccaccgac
     1981 ctcgcggact ggctggtgcg cgaggcgggc gtcccgttcc gcgaggcgca tcatatcgtc
     2041 ggccgggccg tcgccgcggc ggaggagcgc ggagtcggtc tggcggagct cgagctggag
     2101 gcgatgcgcg ggatcgatcc gaggatcgac gagcgggtct tcggcgtcct caccgtcgac
     2161 gcctccgtcc ggagccggac cagctacggc ggcaccgctc cggagcgggt gcgggagcag
     2221 atcgcgaggg cgaagcgggc tctggatttg cctccccatg cgcagcatgg ggagggggac
     2281 cccgcgtagc ggggtggagg ggtaattggc gccgatccgt caggatcgcg ctcgaaccct
     2341 ccagctccgg gcctcttacc cctccaccac cctgcgggtg gtccccctcc ccatctggcg
     2401 atggggaggc agtcaaagcg gcaccagcct cgacagggtc tgcccgctcg gcatcagcag
     2461 atactgggtc gccttggtcc ggacgaaatc gggcaggccc gtcaccgtcc cgccgcccgc
     2521 cggccccggc gggatccgga acaccttcgt cgcccccggc accgccctgt tgccgatcag
     2581 cgcgtcctgg tcgcggccgt gggcggggaa ggcgttgctg aaattgttgc gctggcccca
     2641 ggcggcgag
//
LOCUS       DASWNU010000375         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_323809, whole genome shotgun sequence.
ACCESSION   DASWNU010000375 DASWNU010000000
VERSION     DASWNU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323809"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VGB57_07535"
     CDS             <1..989
                     /locus_tag="VGB57_07535"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0131241.1"
                     /translation="EKSTVAERIGELVMRILAEDLRPSRIITKESLENAIACVCASGG
                     STNGVLHLIALARELGIELTLDDFEQISRQTPLFADLKPGGRFVATDLYAAGGVPVIL
                     KRLAKAGLLHGEQITVTGRTIGEEAAAATEAPGQEVVRQVENPVKAEGGLAILRGNLA
                     PEGSVVKVAGTERRQQTGPARVFENEEECFRAVKDQKIKPGDVVVIRNEGPVGGPGMR
                     EMLQVTAAIVGEGLGESVAMVTDGRFSGATRGLMIGHVAPEAAKGGPIAAIREGDEIT
                     VDIDNRSLGVALSDEEIAERVADYESPQPLYDRGVMAKYAATVSSASEGAVT"
     gene            complement(1007..>1522)
                     /locus_tag="VGB57_07540"
     CDS             complement(1007..>1522)
                     /locus_tag="VGB57_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131242.1"
                     /translation="LISAAGVSFAEVGGRHVEAIGRIVKAATPFLVGDRRAWITRPRG
                     RIFAFGRLFRSPNRLRPELLAEQTRPGLQAPGFSDALESIWGYDTRERLTEIEIPTLV
                     VWGLNDHVVPLEGAIGYHRLIPHSRLEIFERTGHLPMLERPLRFNPMLEEFIESSQPL
                     RSKQDDDITVG"
BASE COUNT          245 a          520 c          549 g          208 t
ORIGIN      
        1 acgagaagag cacggtcgcc gagcggatcg gcgagctggt catgcgcatc ctcgccgagg
       61 acctgcgccc gagccggatc atcaccaagg agtcgctgga gaacgcgatc gcctgcgtct
      121 gcgcctcggg cggttccacc aacggcgtcc tgcacctgat cgcgctcgcc cgcgagctcg
      181 ggatcgagct gacgctcgac gacttcgagc agatctcccg ccagacgccg ctcttcgccg
      241 acctcaagcc cggcggccgc ttcgtcgcca ccgacctcta cgcggccggt ggcgtgccgg
      301 tgatcctcaa gcgcctggcc aaagccgggc tcctccacgg cgagcagatc accgtcaccg
      361 gcaggacgat cggtgaggag gccgcggcgg cgaccgaggc ccccggccag gaggtcgtgc
      421 gtcaggtcga gaacccggtc aaggccgaag gcggtctcgc gatcctgcgc ggcaacctcg
      481 ccccggaggg ctcggtggtc aaggtcgccg gcaccgagcg gcgtcagcag acgggcccgg
      541 cccgggtctt cgagaacgaa gaggaatgct tccgggcggt caaggaccag aagatcaagc
      601 ccggtgatgt cgtcgtcatc cgcaacgagg gcccggtcgg cggccccgga atgcgcgaga
      661 tgctgcaggt caccgcggcg atcgtcggcg aggggctggg cgagagcgtg gcgatggtca
      721 ccgacggccg tttctccggc gccacccgcg ggctgatgat cggccacgtc gcccccgagg
      781 cggcgaaggg cggcccgatc gcggcgatcc gcgagggcga cgagatcacg gtcgacatcg
      841 acaaccgcag cctcggcgtc gccctctcag acgaggagat cgccgagcgc gtcgccgact
      901 acgagtcgcc gcagccgctc tacgaccgcg gcgtgatggc gaagtacgcc gccacggttt
      961 cctccgcctc cgaaggcgct gtcacctaaa cgcgcggcgg cagccgctag ccgacggtga
     1021 tgtcgtcgtc ctgcttcgac cgcaggggct gcgaggactc gatgaactct tcgagcatcg
     1081 ggttgaagcg cagaggtcgc tcgagcatcg gcaggtggcc ggtgcgctcg aagatctcca
     1141 ggcgcgagtg ggggatcagg cggtggtacc cgattgcccc ttcgagcggg acgacgtggt
     1201 cgttgaggcc ccagaccacc agggtcggga tctcgatctc ggtcagcctc tcgcgggtgt
     1261 cgtagcccca gatcgattcg agggcatcgg agaagcccgg cgcctgcagg ccgggtcgcg
     1321 tctgctcggc cagcagctcc ggccgcagtc ggttgggact gcggaagaga cggccgaagg
     1381 cgaagatccg cccccgcggc cgggtgatcc aggcgcggcg gtcgccgacc aggaaagggg
     1441 tcgcggcctt gacgatccgg ccgattgctt ccacgtgcct gcccccgacc tcggcgaagc
     1501 tgactccggc ggcggagatg ag
//
LOCUS       DASWNU010000376         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_376916, whole genome shotgun sequence.
ACCESSION   DASWNU010000376 DASWNU010000000
VERSION     DASWNU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376916"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VGB57_07545"
     CDS             complement(<1..592)
                     /locus_tag="VGB57_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEY0131243.1"
                     /translation="MVQPLMPHATAAWLVDNTSLTFEQIAEFCGLHILEIQAIADDTA
                     ATKLTGRDPIRAGELNQDEIDRGQADPGYKLRILKEPDQTRRTKGPRYTPVSKRQDKP
                     DGIAWIIRNHPEISDGQIGKLIGTTRTTIAAIRDRSHWNIANIQPKDPVTLGLCSQRE
                     LDAIVAKAAKAAGIEPAPTDHRLAGDREALIEELRRE"
     gene            814..1260
                     /locus_tag="VGB57_07550"
     CDS             814..1260
                     /locus_tag="VGB57_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131244.1"
                     /translation="MRKLLIAGLVAAAQVLPAAHPAAAAELGHDRTATSSQISAFAGA
                     RLRVPLGGSREKPQAGLALTSTLRSGATGELRFARGAELGFSGRDSKLDLSLAGRPVS
                     SLAAGRPAPGGRKQGFSTAGWVAIGASAVIIVGGVLIFDYIGDQSE"
     gene            1269..1619
                     /locus_tag="VGB57_07555"
     CDS             1269..1619
                     /locus_tag="VGB57_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131245.1"
                     /translation="MKMRMVMIAALVAAQLSAAGPALAGELPREELATQPGGASAGGR
                     LREDVGRNIGDGQAQEPRPGTAAVSPAPEAAREEAPGKKRKSTGDKVLTGAAVILGVG
                     ALAVGGLLVALFVG"
     gene            complement(1632..>2179)
                     /locus_tag="VGB57_07560"
     CDS             complement(1632..>2179)
                     /locus_tag="VGB57_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0131246.1"
                     /translation="LGVPIERREYDIQKGETRTPQFLATVNPNGRIPVLQIGDRFIPE
                     SNAACFYLADGSALVPEDRFDRADMLRWMFFEQYNHEPNVATLRFWLAFVGEARLNDL
                     QRTLLPGKREAGEAALKLMDEHLAGRSFFVGGRLSLADIALYAYTHVAEEGGFDLARH
                     PAVERWLGRVAAEPGYLAMAS"
BASE COUNT          307 a          698 c          804 g          370 t
ORIGIN      
        1 gctcgcggcg aagctcttcg atcagggcct cgcggtcgcc ggcgaggcga tggtccgtcg
       61 gcgcgggctc gatgccggcc gccttggccg ccttggcgac gatcgcgtcg agctcgcgct
      121 gcgagcagag gccgagcgtg accggatcct tgggctggat gttggcgatg ttccagtggc
      181 tgcggtcccg gatcgcggcg atggtcgtgc gggtggttcc gatcagcttg ccgatctggc
      241 cgtccgagat ctccggatgg ttgcggatga tccaggcgat cccgtcgggc ttgtcctggc
      301 gcttcgatac cggcgtgtag cgcgggccct tggtgcgtcg ggtctggtcc ggctccttca
      361 ggatgcgcag cttgtagccg gggtcggcct ggccgcggtc gatctcgtcc tgattgagct
      421 cgccggcgcg gatcggatcg cgccccgtaa gcttggtcgc ggcggtgtcg tcggcgatcg
      481 cctggatctc gaggatgtgg aggccgcaga attcggcgat ctgctcgaag gtgagcgacg
      541 tgttgtcgac cagccaggcg gcggtcgcat ggggcatgag cggctgaacc acttgtcgtc
      601 tccaaaaaca agaagggccg ccccttcccg gagcggccgt tacgttgcca gcgatctagt
      661 ccagcgagcc gcgcagggca aggcgaatgc cgtctccggc gcccatcatc tttttttcga
      721 caccagcgca tccggggccg catctcccgg catcttgtgg agtgaagctc gacgaggttg
      781 ccgcgactct gcggcctttt ggggggatat cgaatgagaa agttactgat cgccgggctg
      841 gttgcggccg cccaggtcct gccggccgct catccggccg ctgccgcgga gctcggccac
      901 gaccggaccg ccacttccag tcaaatctcc gcctttgccg gagcccggct gcgggtcccg
      961 ctcggcggaa gccgtgagaa gccgcaagcc ggtcttgccc tgacctcgac cctgcgcagc
     1021 ggcgcgaccg gcgagcttcg cttcgccagg ggcgcggagc tcggcttcag cggcagggat
     1081 tcgaagctcg acctgagcct ggcgggccgc cccgtttcca gccttgccgc cgggcgaccg
     1141 gctccgggcg ggcggaagca gggattctcg accgccggct gggtcgcgat cggcgccagc
     1201 gccgtcatca tcgtcggcgg ggtgctgatc ttcgattaca tcggcgacca gagcgaatga
     1261 ggaggcaaat gaagatgagg atggtgatga tcgcggcgct ggtcgccgct cagctttcgg
     1321 ccgccggtcc ggcactggct ggcgaattgc cccgggagga attggcgacg cagccgggcg
     1381 gcgcctccgc gggggggcgc cttcgggagg atgtcgggcg caacatcggc gatgggcagg
     1441 cgcaggagcc tcgcccggga accgcggcgg tgtcgccggc ccccgaggcc gctcgggagg
     1501 aggcgcccgg gaagaagcgc aaaagcaccg gcgacaaggt gctgacgggc gccgccgtga
     1561 tcctgggggt cggcgccctg gccgtcgggg ggctgctcgt cgcgctcttc gtcggctgag
     1621 gcccgatccg cctacgatgc catggcgagg tagccgggtt cggccgccac tcttcccagc
     1681 cagcgctcga ccgcggggtg gcgggcaaga tcgaagccgc cctcctcggc cacatgggta
     1741 taggcgtaga gggcgatgtc ggcgagactg agtcgcccgc cgacgaagaa gctccggccg
     1801 gcgagatgct cgtccatcag cttgagcgcg gcctcaccgg cctcgcgctt gccgggcagc
     1861 agggttcgct ggagatcgtt cagccgcgcc tcgccgacga aggccagcca gaagcgcagg
     1921 gtcgcgacgt tgggctcgtg attatattgc tcgaagaaca tccagcgaag catgtcggcg
     1981 cggtcgaacc ggtcctcggg caccagcgcc gacccgtcgg cgagatagaa gcaggcagcg
     2041 ttgctttcgg ggatgaagcg gtccccgatc tggaggaccg gtattcgccc gttgggattg
     2101 accgtggcga ggaattgcgg ggtccgggtc tcgcccttct ggatgtcgta ttcccgccgc
     2161 tcgatcggga ccccgagca
//
LOCUS       DASWNU010000377         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_482451, whole genome shotgun sequence.
ACCESSION   DASWNU010000377 DASWNU010000000
VERSION     DASWNU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482451"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VGB57_07565"
     CDS             complement(<1..1156)
                     /locus_tag="VGB57_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEY0131247.1"
                     /translation="MLESEGRPLTFLEITEKSIAQNLLSHVGKTPELTMLSRLAAMAR
                     RTRDRKVVVTAKDTFALADWALPEDAEALALTGVMEAHPEDSLPPLRPVERHPEPRTD
                     NVRVAGRGSERKRRRDEEEEAGRGGRRRRFPPIPEVVFEILSESDQALRPDQILEQAR
                     TKELAAEVRAAEAAQAEAEVAWQTAIMSIPNLAHEQAPAGGEDDYTVLETVGTPRDFE
                     AEGFEPRDHVELGRILGAIDMERGAKVSGSRFYFLTGVGAQLELALVNLAMEQARAAG
                     FTQVVAPSLVRPRAMEGTGFLGQAADDVYRIEGEDLYLVGTSEVPMAAYHSDEILDAA
                     SLPRRYAAYSPCYRKEAGSYGKDTSGIIRVHWFDKVEMFTYTTVEESYAEH"
     gene            1287..>1756
                     /locus_tag="VGB57_07570"
     CDS             1287..>1756
                     /locus_tag="VGB57_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131248.1"
                     /translation="MIRFRIGHRWKREPAEPPHDSVSLELDGVNLLPGAVEEPLVEAV
                     PALVEAVAGLLAGGRRLAQVSLTEAHLELVLRRSGTDIELQVASLSRPARLLRPPLRL
                     DADELAEAARGCGQGFLEDLRKVAPRRLPAAVEQALERALRQLGEPVHFPEELRP"
BASE COUNT          254 a          592 c          629 g          281 t
ORIGIN      
        1 ggtgctccgc gtaggactcc tcgaccgtcg tgtaggtgaa catctcgacc ttgtcgaacc
       61 agtgcacccg gatgatgccg ctggtgtcct tgccgtagga gcccgcctcc ttgcggtagc
      121 aggggctgta ggccgcgtac cgccgcggca gcgacgccgc gtcgaggatc tcgtcgctgt
      181 ggtacgccgc catgggcact tcggaggtgc cgaccaggta caggtcctcc ccctcgatcc
      241 ggtagacgtc gtcggccgcc tgaccgagga agccggtgcc ctccatcgcc cgcggccgca
      301 ccagtgacgg cgcgacgacc tgggtgaagc cggcggcccg ggcctgctcc atcgcgaggt
      361 tgacgagcgc gagctccagc tgagccccga ctccggtgag gaagtagaac cgggagccgg
      421 agaccttcgc cccacgctcc atgtcgatcg ccccgaggat ccggccgagc tccacgtggt
      481 cccggggctc gaagccctcc gcctcgaagt cgcggggggt gccgacggtc tccaggacgg
      541 tgtagtcgtc ctcgccgccg gcgggcgcct gctcgtgcgc gaggttgggg atcgacatga
      601 tcgcggtctg ccaggccacc tcggcctcgg cctgtgcggc ctccgccgcc ctgacctcgg
      661 cggccagctc cttggtccgc gcctgctcca gaatctggtc cgggcgcagg gcctggtcgg
      721 actcgctgag gatctcgaac accacctccg gaatgggcgg gaagcggcgg cggcgtcctc
      781 cccgcccggc ctcctcctcc tcgtcccggc gacgcttgcg ctcgctgcca cggccggcca
      841 cccgcacgtt gtccgtgcgc ggctccgggt gacgctcgac cggccgcagg ggcggcaggc
      901 tgtcttctgg atgggcttcc atcacccccg tcagcgccag cgcctcggca tcctccggca
      961 acgcccaatc ggcgagggcg aaggtatcct tggcggtgac gaccaccttg cgatcgcgcg
     1021 tcctgcgagc catcgccgcg agccgcgaca acatcgtcag ctcgggcgtc ttgcccacgt
     1081 gggacagcag gttctgggcg atcgacttct cggtgatctc gaggaaggtg aggggacgtc
     1141 cttcgctctc aagcacgcgg agagcggcct cataaaaagt catcggcgaa ttccccaaca
     1201 aaatcgaacg gttacaaaaa tgtaggtccg tataggcggc ggatgctagc catcgctatt
     1261 ctggcttgtc aacgaatggg gaagggatga tccgctttcg catcggacat cggtggaagc
     1321 gcgaacccgc ggagccgccg cacgattccg tttcgctgga gctggacggg gtcaatctgc
     1381 tgccgggagc agtggaagaa ccgctcgtgg aagcggtgcc ggcgctcgtg gaggctgtgg
     1441 cggggttgct cgcgggaggg cggcggctgg cgcaggtgtc gctgacggag gcgcacctgg
     1501 agctggtgct gcgacgttca ggaacggaca tcgagctaca ggtggcgagc ctctcgaggc
     1561 cggcccggct gttgcgcccg ccgctgcggc tggacgcgga cgagctggcc gaggcggccc
     1621 gggggtgcgg ccagggtttc ctggaggacc tccgaaaggt ggccccccgg aggctaccgg
     1681 cggccgtcga gcaggcgctc gagcgggcgc tgaggcaatt gggggagccg gtgcacttcc
     1741 cggaggagct gcgccc
//
LOCUS       DASWNU010000378         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_351346, whole genome shotgun sequence.
ACCESSION   DASWNU010000378 DASWNU010000000
VERSION     DASWNU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351346"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGB57_07575"
     CDS             <1..430
                     /locus_tag="VGB57_07575"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339111.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY0131249.1"
                     /translation="GQRHRLGMAFNEMVASGELKAPVVIGRDHLDSGSVASPNRETEA
                     MRDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGYSQHAGMVIVADGTPEAERR
                     LERVLWNDPGTGVMRHADAGYEEAIDCAREQGLDLPMVGS"
     gene            519..2036
                     /gene="hutH"
                     /locus_tag="VGB57_07580"
     CDS             519..2036
                     /gene="hutH"
                     /locus_tag="VGB57_07580"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY0131250.1"
                     /translation="MIILRPGSVTLAELRSIWAGAPVQLAHDAWAAVDAAAACVGRIV
                     ASGRTVYGINTGFGLLAQTRIPGERLAELQRNLVMSHSCGLGEDLSRPVVRLAMATKA
                     IGLARGHSGVRREVVERLLALLEADALPCIPSQGSVGASGDLAPLGHLSAAMLGEGRI
                     DLRGTVMPAGEALRELGLEPLELGPKEGLALINGTQISTAVALDTLFAAERIFSSALI
                     SGALSLDALKGTDAAFDPRIHEARGQKGQIAVAAVLKRLIEGSEIRHSHDDCTRVQDP
                     YSFRCQPQVMGACLDLLRNVTESLRIEANAVTDNPLLFPETDDSLSGGNFHAEPVAFA
                     ADTLAMALCEVASISERRTAVLVDPKMSGLPPFLVRDSGVNSGFMIAQVTAAALVSEN
                     KSLAWPASVDTVPTSGNQEDHVSMATHAGTKARRIAGNSAGVVGIELLAAAQGCDFHA
                     PLKSSPALETARADIRRKVEHYEADRYLADDLRWAQDAVLAGSLSGEVEAELFGR"
     gene            2131..3696
                     /locus_tag="VGB57_07585"
     CDS             2131..3696
                     /locus_tag="VGB57_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0131251.1"
                     /translation="MIDLAQIPGPLELEGEVCVIGAGAAGISLTRRLLALGHGVVLLE
                     SGGLDYEAATARLNAGENSGEDYYELEDSRLRFFGGTTAIWGGRCAELDPVDLERRDY
                     VPHSGWPIGWQELERWYKEARPAFGLPEGRRGPDDLRSAGVKLPRFEALETPLWTFDR
                     RFNRFAWESCADLEADPRCQIATHATVTAIVPEGRGISRVEARSLGAGRLTVRAKAFV
                     LAAGGIENPRLLLASGLGNGRDQVGRYFMEHPHARGGRIVKGAAWPLLKAFGRRHRVA
                     GHDLAALIAPSEALQRREGILNTSLTIVARQPQGRRQFIGMKAYSGLKHNMAPTRRGR
                     ALWMATKKAAGWAQRHIDPARPWLLHKIGNLEVALLVRGEQAPNPASRVTLIGDKDST
                     GVPRVKLDWRLSELDVRSVAVLVETLGRELDRLGLGKVEPAEWLGSGEWKTDPLISSH
                     PIGGYHHMGTTRMGSDPATSVTDRDGRVHGIGNLWVAGSSLFPTGGWANPTLTIVALA
                     LRSAEMISRELKA"
BASE COUNT          555 a         1220 c         1382 g          580 t
ORIGIN      
        1 cggccagcgc caccggctcg gaatggcgtt caacgagatg gtcgcctcgg gcgaattgaa
       61 ggcgccggtg gtcatcggcc gcgaccatct cgattccgga tcggtggcga gccccaaccg
      121 ggagaccgag gcgatgaggg acggctccga cgccgtttcc gactggcccc tgctcaacgc
      181 cctcctcaac accgcctcgg gcgcgacctg ggtgtcgctc caccatggcg ggggagtcgg
      241 catgggctac tcccagcatg ccgggatggt gatcgtcgcc gacggcactc cggaagcgga
      301 gcggaggctg gagcgggtgt tgtggaacga cccggggacc ggagtgatgc gccatgccga
      361 tgcgggctac gaggaggcga tagattgcgc gcgggagcag gggctcgacc tgccgatggt
      421 gggatcgtga gcgtcatccc ggcggaagcc gggacctcgg ggagacccgc cgagacttct
      481 cgacctgagg tcccggcttt cgccgggatg acggttcgat gatcattctc cgtcccggct
      541 cggtaaccct cgccgagctt cgctcgatct gggccggtgc tcccgtccag ctcgcccacg
      601 acgcctgggc cgccgtcgac gccgccgccg cctgcgtcgg gcggatcgtg gcgagcgggc
      661 gaaccgtcta cggaatcaat accggcttcg gactgctcgc ccagacccgt attccgggcg
      721 agcggctggc ggagcttcag cgcaacctcg tcatgtcgca cagctgcggg ctcggcgagg
      781 acctcagccg gccggtggtt cggctggcga tggcgaccaa ggcgatcggg ctcgcccgcg
      841 gccattcggg cgtaaggcgc gaagtggtgg aacggctgct ggccctgctc gaggcggacg
      901 ccttaccgtg catcccgtcc cagggctcgg tcggcgcttc gggcgatctc gctccgctcg
      961 ggcatctcag cgcggcgatg ctcggcgagg ggcggatcga cctcaggggc acggtcatgc
     1021 cggccggcga agctttgcgc gagcttgggc tggagccgct ggagctcggc ccgaaggaag
     1081 ggctggctct gatcaacggc acccagattt ccaccgcggt ggcgctcgac accttgttcg
     1141 cggccgagcg gatattctct tcggcgttga tttcaggggc tttgtcgctc gacgccctga
     1201 agggcaccga tgccgccttc gacccgagga tccacgaggc gcggggccag aaggggcaga
     1261 tcgcggtggc ggcggtgctg aagcggctga tcgagggaag cgagatccgc cacagccacg
     1321 acgactgcac gagggtgcag gatccgtaca gcttccgctg ccagccgcag gtgatggggg
     1381 catgtctgga cttgttgagg aatgtcacgg aatcgcttcg aatcgaagcg aacgcggtca
     1441 ccgacaaccc tttgttgttc ccggagacgg acgactcgct gtcgggcggc aatttccacg
     1501 ccgagccggt ggccttcgcc gccgacaccc tggcgatggc gctgtgcgag gtcgcgagca
     1561 tctcggagcg gcggacggcg gttctggtag accccaagat gagcgggctg ccgcccttcc
     1621 tggtccggga cagcggcgtg aattcgggct tcatgatcgc ccaggtgacg gctgcggcgc
     1681 tggtttccga gaataagagc ctggcctggc cggcgagcgt cgacacggtg ccgacctcgg
     1741 gcaaccagga ggaccatgtc tcgatggcga cccacgccgg caccaaggcc cggcggatcg
     1801 ccggcaattc ggcgggggtg gtggggatcg aattgctggc ggcggcgcag ggctgcgact
     1861 tccatgcccc cctgaagagc tcgccggcgc tggagacggc gcgggcggat atccggcgca
     1921 aggtcgagca ttacgaagcg gaccgctatc ttgccgacga tctgcgctgg gcccaggacg
     1981 cggtcctggc cgggagcctg tcgggcgagg tcgaggcgga gttgttcgga aggtgagctc
     2041 tccccgtcat cccggcggag gccgggacct caggtcgagg agtcacggcg ggtctccccg
     2101 aggtcccggc tttcgccggg atgacggatt gtgatcgacc tcgcgcagat ccccggccct
     2161 ctcgagctgg agggcgaggt ctgcgtgatc ggcgcgggcg cggcggggat cagcctgacc
     2221 cggcggctgc tcgcgctggg gcatggcgtc gtgctgctgg agagcggcgg gctcgactat
     2281 gaggcggcga ccgcccggct caacgccggc gagaattcgg gcgaggatta ttacgagctc
     2341 gaggacagcc gcctgcgctt cttcggtgga accaccgcga tctggggcgg gcgctgcgcg
     2401 gagctcgatc cggtggacct cgagcggcgc gactatgtgc cgcattcggg atggccgatc
     2461 ggctggcagg agctggagcg ctggtacaag gaggcccgcc ccgccttcgg ccttccggaa
     2521 gggcggcggg ggccggacga cctgcgctcg gcgggcgtga agctgccgcg cttcgaggcg
     2581 ctcgaaaccc cgctctggac cttcgaccgc cgcttcaacc gcttcgcctg ggaaagctgc
     2641 gccgatctcg aggccgatcc cagatgccag atcgcgaccc acgcgacggt gacggcgatc
     2701 gtccccgagg gccggggcat ctcccgggtc gaggcgcgct cgctcggcgc cggccggctg
     2761 acggttcggg ccaaggcctt cgtgctggcc gcgggcggga tcgagaatcc gaggcttctg
     2821 ctcgcctccg gcctcggcaa cggccgcgac caggtcggcc gctatttcat ggagcatccg
     2881 catgcccgcg gcgggcgaat cgtcaagggc gcggcctggc ccctgctcaa ggcgttcggg
     2941 cggcggcacc gggtggccgg gcatgacctc gccgccctca tcgcgccgtc ggaggcgctg
     3001 cagcgccgcg agggcatcct caacacctcc ctgaccatcg tcgcccgcca gccgcagggc
     3061 cgccggcagt tcatcgggat gaaggcctat tccggcctca agcacaatat ggcgcccacc
     3121 aggcgagggc gggccttgtg gatggcgacc aagaaagccg ccggatgggc gcagcgccat
     3181 atcgacccgg cgcggccctg gcttctccac aagatcggca atctcgaagt cgccttgctg
     3241 gtgcgcggcg agcaggcgcc caatccggcg agccgggtga ccctcatcgg cgacaaggat
     3301 tcgaccggag tgccccgggt gaagctggac tggaggcttt cggagctgga cgtgaggagc
     3361 gtcgcggtgc tcgtcgagac gctcgggcgg gagctggacc ggctcgggct cggcaaggtc
     3421 gagccggccg agtggctcgg ctcgggcgaa tggaaaaccg atccgctcat ctcgtcgcac
     3481 ccgatcggcg gctatcatca tatggggacg acccgaatgg ggagcgatcc ggcgaccagc
     3541 gtcaccgatc gggacgggcg ggtgcacggc atcggcaatc tgtgggtggc gggctcgtcg
     3601 ctcttcccca ccggcggctg ggccaacccg accttgacca tcgtcgcttt ggcgctgcgc
     3661 agcgccgaga tgatttcccg ggagcttaag gcgtgacagc ggttgcgccg gtcggcgcca
     3721 atccgaccct actgtgg
//
LOCUS       DASWNU010000379         5588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_324156, whole genome shotgun sequence.
ACCESSION   DASWNU010000379 DASWNU010000000
VERSION     DASWNU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324156"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..4288
                     /locus_tag="VGB57_07590"
     CDS             125..4288
                     /locus_tag="VGB57_07590"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HEY0131252.1"
                     /translation="MVSISSNGGGATASFSLGENQNVATTVTATGSGAITYAISGGLD
                     ALSFTIDAQTGVLSFLSAPNFEAAGDSGANNVYDVEVTASDGTLSASQALAITITNVN
                     EAPVFTSSTSLVSPENSYAIALLAATDPDGDPLTFSTPNGEDWFRLHVTPSTGQLSFV
                     TAPNFEAPADYGQDGTYKIDVRVSDGTLFSTQTFSVTIGNVNEGAMISSNGAGATAAF
                     SVSENQLVATTVVGRDNPVGTTLAYAITGGNDSSRFTINAQTGVLSFVNAPNFESPHD
                     SNFNNVYDVIVSATDGTVTDTQALAITVTNVNEPISIISEGGGDTAAFSLLENGGGFT
                     VVTADQDAATPGFSIIGGADAARFQFLTAASKTGWFGMVSPPNYEAPTDSNGDNVYEL
                     IVQASDGLNTDVQTLYITITNAQEAVLITSNGAGSSASITVAEGQPAVTVVAATDPDN
                     QAVAYSISGGVDAARFAIDSATGALSFVAAPDYELPNDSGANRIYDVIVRASDGVTTD
                     TQALAISISNVIEPFAITTHGGGDTGALAVNEGALRTFTVATNEASDLTFSLSGADAA
                     RFTILGSVGGSRTISFAAPPNYEAPTDAGADNVYDVVVTVSGGGYSDSQALAISVGNI
                     DEVPVITSNGGGATAFFSRAENGSATVTTVMAVDAENAAVTYGISAGADAARFTIDSA
                     TGVLAFVATPDFETPTDAGGDNVYDVTVWASDGTYSDGQTITVTVTNVEEGGPAITSN
                     GGGTWASVSVAENSFAVATVAAAQTPPVNGPITYSIAGGADSARFAIDSATGALTFAT
                     APNFEVRSDVGANGVYDVTVRASDGVNADVQQLIVTVTNVNEAPVFTTGAVVAANENS
                     LVVGSIMTTDPENNPRSYSIVGGADSAKFTINSVNGTLSFVAAPNFELPTDSDLNNGY
                     EVVVRASDGTLATNQTVNVLVNNLNEAPIITSNGAGTNASLSIGENSTGVTTVVAADP
                     ENTAITYSISGGADSSKFTINASTGALAFVTAPNFESKTDVGANGVYDVIVRASDGFN
                     VDTQAIAITVTNVNEAPVITSNGGGATASVSLGENGSTVTVVASTDPEGTARTYSIAG
                     GADAARFTMSSNGILSFIAAPDFESPTDAGGNNVYDVVIRASDGALDDTQAMAVTVTN
                     LNEAPVITSDGAGTTGAVTVSENIALVTTVTSTDPENATRTYSISGGLDSAKFAINAS
                     TGVLSFVTAPNFESPTDTNADNQYFVTVRSSDGVFADTQTLTVTVANVNEAPVITSNG
                     GGASASVQLLENGLAVTTVQASDVDGPTGSYAIAGGADAALFSIDLATGALSFLAAPD
                     YETPADADGDNVYQVTVSASDGSLFDLQDLSVSVGDLFEGGGQAFPAPELSSGAHYPP
                     TPVMLEYLV"
     gene            4507..>5588
                     /locus_tag="VGB57_07595"
     CDS             4507..>5588
                     /locus_tag="VGB57_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131253.1"
                     /translation="MPTGTEGPDDFLITSIETYHGLGGDDIFNLFADIAFGTTPKFLR
                     VDGGSGFDTLRITASLVRELYVPFPPYQGSVWVNGSSAGGTVVDYGNIERIYVSSTAD
                     FRGYPIGQPKAGAWSTSDTIDEIHVLGVVGNHAISVFTGGSDDKIYLGPVGAGSAGHG
                     GSGNDLVDLSGVLAGSASAFGDEGDDILVGSAQSDTLDGGSGVDSMSGGGGDDIYIVD
                     NSGDTIVEAADAGLDTARVAAASYVLSDGVENLVATDSGPHNFTLNAGDNAVTGNDGA
                     DLLRLQQGGADSAAGHGGNDIFYLGGALDPTDALDGGSGTDTLVLQGDYRFGVLLGEN
                     ISSIENVSMLAGSNTAFGDPGTNLYE"
BASE COUNT         1037 a         2007 c         1649 g          895 t
ORIGIN      
        1 cgcaatatcc cgaagcatcg tcgacgcgat cctggaaagg cctcggatat ccgtattttc
       61 gtttaccgcg cataaaagaa cccggtgcta gcctcggtcg gagaataata acggggaaag
      121 ccaaatggtc tcgatttcat cgaatggcgg cggcgcgacc gcatctttct cgctcggcga
      181 gaatcagaac gtagcgacga cggtcaccgc cacgggcagc ggcgcaatca cttacgcgat
      241 ctcgggtggc ctcgacgccc tctcattcac catcgatgcg cagacaggcg ttctgagctt
      301 cctcagcgct cccaatttcg aggcggcggg cgattccggc gcgaacaacg tctacgacgt
      361 cgaggttacg gcttccgacg gaacgctgag cgccagccag gcgctggcga tcacgatcac
      421 caacgtcaac gaggcgccgg tcttcacgtc ctcgaccagc ctcgtctcac cggagaacag
      481 ctatgccata gccctcctcg ccgcgaccga tccggacggc gatccgctca ccttttcgac
      541 ccccaacggg gaagattggt tcagactgca cgtcaccccc tcgacggggc agctgagctt
      601 cgtgacggcg ccgaatttcg aggcgccggc cgattacggc caggacggaa cctacaagat
      661 cgacgtccgc gtcagcgacg gcacgctctt ttccactcag actttctcgg tcaccatcgg
      721 caacgtcaac gaaggggcga tgatctcgtc caacggcgcc ggcgcgaccg ccgccttctc
      781 ggtctcggaa aaccagctcg tcgcgacgac cgtggtcggt cgggacaatc cggtcggaac
      841 gacgctcgcc tacgccatca ccggcggcaa cgattcctcg cgcttcacga tcaacgccca
      901 gaccggagtc ctcagcttcg tcaacgcgcc caatttcgaa tcgccgcacg attcgaactt
      961 caacaacgtc tacgacgtca tcgtctccgc caccgacggc accgtcaccg acacgcaggc
     1021 gctcgccatc accgtcacca acgtcaacga gccgatctcg atcatctccg aaggcggcgg
     1081 cgataccgcc gctttctcgc tgctcgaaaa cggcggcggc ttcaccgtcg tcaccgccga
     1141 tcaggatgcc gccactccag gcttctcgat catcggcggc gccgacgccg cgcgcttcca
     1201 gttcctcacc gccgccagca agacgggctg gttcggcatg gtctccccgc ccaattacga
     1261 ggcgcccacc gactccaatg gcgacaatgt ctacgagttg atcgtccagg cctccgacgg
     1321 cctcaacacc gacgtccaga cgctctacat caccatcacc aatgcccagg aggcggtgct
     1381 catcacctcc aacggagccg gcagcagcgc cagcatcacc gtggccgagg gccagccggc
     1441 ggtgaccgtc gtcgcggcca ccgatccgga caaccaggcc gtcgcctaca gcatctccgg
     1501 cggagtcgac gccgcccgct tcgccatcga ctcggcgacc ggcgctctga gcttcgtcgc
     1561 cgcgcccgac tacgagcttc ccaacgattc cggagccaac cggatctacg acgtgatcgt
     1621 ccgggccagc gacggggtga ccaccgacac gcaggcgctc gcaatcagca tctccaacgt
     1681 gatcgagccc ttcgccatca ccacgcacgg cggcggcgat accggcgctc tggcggtcaa
     1741 cgagggggcc ctacggacct tcaccgttgc caccaacgaa gcttcggacc tgaccttcag
     1801 cctcagcggc gccgacgccg cgcgcttcac catcctcggg agcgtcggcg gaagccggac
     1861 gatcagcttc gccgctccgc ccaattacga ggcgcccacc gatgcggggg cggacaatgt
     1921 ctacgacgtc gtcgtcacgg tctccggggg cggctacagc gacagccagg cgctcgcgat
     1981 cagcgtcggc aacatcgacg aagtgccggt catcacctcg aacggcggcg gcgccaccgc
     2041 attcttctcc cgcgctgaga acggctccgc caccgtcacg accgtgatgg cggtcgatgc
     2101 cgaaaatgcc gccgtgacct acggcatctc cgccggcgcc gatgcggcca ggttcaccat
     2161 cgactccgcc accggcgtgc tcgccttcgt cgcgaccccg gacttcgaga cccccacgga
     2221 tgccgggggc gacaacgtct acgacgtcac cgtctgggcg agcgacggca cctattcgga
     2281 cgggcagacg atcaccgtca ccgtcaccaa cgtcgaagag ggcggcccgg ccatcacctc
     2341 gaacgggggc gggacctggg cctcggtctc ggtcgccgag aatagtttcg cggtcgccac
     2401 cgtcgcggcc gcccagaccc cgcccgtgaa cggcccgatc acctactcca tcgccggcgg
     2461 ggcggactcg gccaggttcg cgatcgactc cgcgacggga gcgctcacct tcgccaccgc
     2521 gcccaatttc gaggtccgct ccgacgtcgg cgccaacgga gtctacgacg tcaccgtgcg
     2581 cgccagcgac ggcgtgaacg cggacgtcca gcagctcatc gtcaccgtca ccaacgtcaa
     2641 cgaggcaccg gtcttcacca ctggcgcagt ggtggcggcc aacgagaaca gcctggtcgt
     2701 cggctccatc atgaccaccg atcccgagaa caacccgcgc agctattcga tcgtcggcgg
     2761 ggccgactcg gcgaaattca cgatcaattc ggtgaacggc acgctcagct tcgtcgccgc
     2821 gcccaatttc gagcttccca cggattcgga cctcaacaac ggctacgagg tggtcgtccg
     2881 cgccagcgac gggaccctgg ccacgaacca gactgtcaac gtcttggtca acaacctaaa
     2941 cgaggcgcca atcatcacct ccaacggcgc gggcacgaac gcctcgctct ccatcggcga
     3001 gaattcgacc ggggtgacga ccgtggtcgc cgccgatccc gagaacaccg ccatcaccta
     3061 cagcatctcg ggcggggccg actcgtccaa gttcaccatc aacgcctcca ccggcgccct
     3121 ggccttcgtc accgcgccca atttcgagtc gaaaaccgac gtcggcgcga acggggtcta
     3181 cgacgtcatc gtccgcgcca gcgacggctt caacgtcgat acgcaggcga tcgcaatcac
     3241 cgttaccaac gtcaacgagg cgccggtgat cacctcgaac ggcggcggcg ccaccgcgtc
     3301 cgtcagcctc ggcgagaacg gctcaaccgt caccgtcgtc gcctcgaccg atcccgaagg
     3361 gacggcgcgc acctattcga tcgccggggg cgccgatgcc gcgcggttca cgatgagcag
     3421 caacggcatc ctgagcttca tcgccgctcc cgatttcgag agcccgaccg acgccggcgg
     3481 caacaacgtc tacgacgtcg tgatccgggc gagcgacgga gcgctcgacg acacccaggc
     3541 gatggccgtc accgtcacca acctcaacga agcgccggtc atcacctcgg acggcgccgg
     3601 caccacgggc gccgtgaccg tgagcgagaa tatcgccctc gtcaccaccg tcacctcgac
     3661 cgacccggag aatgccaccc gcacctattc gatctcgggc ggcctcgatt cggcgaagtt
     3721 cgcgatcaac gcctcgacag gagttctcag cttcgtcacg gcgcccaatt tcgaaagccc
     3781 gacggacacc aatgccgaca accaatattt cgtcacggtg aggtcgagcg acggagtgtt
     3841 cgccgacacc cagaccctca ccgtcaccgt cgccaacgtg aacgaagcgc cggtcatcac
     3901 ctccaacggc ggcggcgcct ccgcgagcgt ccagctcctc gagaacggcc tcgccgtcac
     3961 caccgtccag gcgagcgacg tcgacggtcc gaccgggtcc tacgccattg ccggcggcgc
     4021 cgacgccgcc ttgttctcga tcgacctggc gaccggcgcc ctgagctttc tcgccgcgcc
     4081 cgattacgag accccggccg atgccgatgg cgacaacgtc tatcaggtga cggtgtcggc
     4141 gagcgacggg agccttttcg acctccagga cctgtcggtc tcggtcggcg acctgttcga
     4201 gggcgggggc caggccttcc cggcccccga attgtcgagc ggcgctcatt atccgcccac
     4261 tccggtgatg ctcgaatatt tggtctagtc ggtccgtttt ggcaggcgga agggggcagt
     4321 ccaggcgatt gtccccttcg tcagacgcca accgagcttc tcaagatacg gagattacat
     4381 ccgttttgcc cctaactccg cttaggacag gggcggggcc atgttactct cggcgtcgcg
     4441 cgatgaccgg gcggagccgg cgctgttccg ccgcgaccgc gttgtgcgac ggcaggagta
     4501 aagaccatgc ccaccggcac cgagggaccc gacgacttcc tgatcaccag catcgagacc
     4561 tatcatggtc ttggcggtga cgatatattc aacctctttg ccgacattgc cttcggaaca
     4621 acgcctaagt ttttgagggt cgacggggga tcgggcttcg acaccctccg aataaccgcc
     4681 tctcttgtcc gcgagctgta cgttcctttc cctccctatc agggatcggt atgggtaaat
     4741 ggctcgagcg ctggcggcac cgtcgttgat tacgggaaca tcgagcggat ctacgtctcc
     4801 agcactgccg attttcgcgg ctatccgatc gggcagccca aggccggcgc ctggtcgacg
     4861 agcgacacga tcgacgaaat ccacgtcctg ggtgtcgtcg gcaatcatgc gatcagcgtc
     4921 ttcaccggcg gcagcgacga caagatctat ctggggccgg tcggcgccgg atcggccggg
     4981 catggcggga gcgggaacga ccttgtcgac ctgagcggcg tcctggcggg ctccgcatcg
     5041 gctttcggcg acgaaggcga cgacattctg gtcggaagcg cccagtcgga cacgctcgac
     5101 ggcggctccg gagtcgattc gatgagcggc ggtggcggcg acgatatcta catagtcgat
     5161 aattccggcg acacgattgt cgaagccgcg gatgccggcc tcgacacggc gagggtcgcc
     5221 gccgcctcct acgtcctctc ggacggagtc gagaatctgg tcgcgaccga ttccgggcct
     5281 cataacttca cgctcaacgc cggcgacaac gcggtcaccg gcaacgacgg cgccgatctc
     5341 cttcgccttc agcaaggcgg cgccgactcc gccgccgggc atggcggcaa cgacatcttc
     5401 tacctcggcg gggcgctcga tccaacggac gccctcgacg gcgggagcgg cacggacacg
     5461 ctcgtcctcc agggcgatta ccgtttcggt gtgctgctcg gcgagaacat ctcgagcatc
     5521 gagaacgttt cgatgctcgc cgggagcaac accgccttcg gcgacccggg gacgaacctc
     5581 tacgagta
//
LOCUS       DASWNU010000380         4683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_299547, whole genome shotgun sequence.
ACCESSION   DASWNU010000380 DASWNU010000000
VERSION     DASWNU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299547"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGB57_07600"
     CDS             <1..461
                     /locus_tag="VGB57_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0131254.1"
                     /translation="AGSGYSWQDAFGTGDRAEVERHCAAIGAEHEWLGGDVLRVMEVR
                     PATAVHPATGEEIWFNQADGFHPSALGPATYAELLAQCGSEDRFRLNVSYGDGTPIAP
                     PDLAAVRQVIRRETRPHIWQSGDILMLDNLLAAHGRRPFSGERRIAVAMS"
     gene            632..889
                     /locus_tag="VGB57_07605"
     CDS             632..889
                     /locus_tag="VGB57_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131255.1"
                     /translation="MTLYFLHLRDGTDIVLDEEGSDHDSLASLSAEMLAAARDVMRED
                     VQHGRLDLRLRVDAETADGALARSLSFVEALEIVFPEPESA"
     gene            complement(900..1979)
                     /locus_tag="VGB57_07610"
     CDS             complement(900..1979)
                     /locus_tag="VGB57_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0131256.1"
                     /translation="MTRLGFIGTGWIGLDRMRAMLAIEGVEAVAICEPNPAMAAEALK
                     LAPDAALVTSLDEMLALAPDGVVIATPSALHAAQCLRVLEAGAAVFCQKPLGRNAAEV
                     EQVLAAARRADRLLGVDLSYRYTEGMRAVRELIGSGALGTVYAANLVFHNAYGPDKAW
                     FYDLELSGGGCVIDLGIHLVDLALWTLDFPQVTGVSSRLYSKGRPLEAGGIEDFASAT
                     LDLEDGATVQVACSWNLAAGSDAVIGAEFYGTEGGAAFRNVDGSFYDFTAERLNGTRR
                     ERLADPPDAWGGRAAAHWAQSLAQGSRWSGSTIGLSEAATAIDRIYGASGRGGTAGTG
                     PATAEASAPQQPCIAAADQAVPTLG"
     gene            complement(1988..2947)
                     /locus_tag="VGB57_07615"
     CDS             complement(1988..2947)
                     /locus_tag="VGB57_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0131257.1"
                     /translation="MMRAAVLTGPGRLRIEEVAKPSPGPGQVRIRLEGCGVCASNLTP
                     WAGPDWMTFPTEPGTLGHEGWGRIDAVGEGVEGIAEGVRVAALSGRAYAEYDLADAAG
                     IVELPSELDGLAFPGEPFGCAMNIFRRSGIEAGQTVAIVGIGFLGALLTRLSSDAGAH
                     VIAISRRPCSLALAKAMGAAETIAMDDHYAIIDQVRALTNGLFCDCVIEAVGKQWPLD
                     LAAELTRERGRLVVAGYHQDGPRQINMQLWNWRGFDVVNAHERDPAVSVRGMREAAAA
                     AAAGRIDPEPLLTHLYGLDGLGTALDATRDRPPGFVKALVTFP"
     gene            complement(2944..3477)
                     /locus_tag="VGB57_07620"
     CDS             complement(2944..3477)
                     /locus_tag="VGB57_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY0131258.1"
                     /translation="MSQGPTVPPPGPDSLNRALARNIAALRERERRDRSRTTFQERLA
                     EAITRFTGSMRFVYVHLALYGGWIVANLGWIPGFEPWDPTFVVLAMIASVEAIFLSTF
                     VLISQNRMAAEAERRATLDLQINLLAEHEVTRVITLVSAIADRLGVDSPAPDEEIQEL
                     KRDVAPEAVLDAIEEEK"
     gene            complement(3474..4520)
                     /locus_tag="VGB57_07625"
     CDS             complement(3474..4520)
                     /locus_tag="VGB57_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY0131259.1"
                     /translation="MKKPKQKIALVTGGAGFIGSHICDALIAEGARVVCLDSFLTGRR
                     ENIRHLEGESRFELVECDVIDPLPWRIRGGRIPFTHIYHLACAASPPHYQADPEHTMM
                     TNVVGTQSMLRLAEVNGARLLLTSTSEVYGDPEVHPQTEDYRGWVSCTGPRACYDEGK
                     RAAETLAFDYQRSGRCEVRVARIFNTYGPRMRPDDGRVVSNVICQALAGDDITLYGDG
                     SQTRSFCYVSDLVDGLLRLMESEKAVGFPVNLGNPNELTVEDLVHRVLAMTGSRSAIV
                     NRPLPEDDPRRRKPDIGRAAELLGWAPRVGLQEGLEATIAWFAGAGATAEPWHGIGRV
                     RPGFAETRLIQAAE"
BASE COUNT          715 a         1613 c         1634 g          721 t
ORIGIN      
        1 gcgcgggctc cggctattcc tggcaggatg ccttcggaac cggcgaccgg gcggaggtgg
       61 agcgccattg cgcggcgatc ggagcggagc atgaatggct cggcggcgat gtcctgcggg
      121 tgatggaggt caggcccgca acggccgtcc atccggcgac cggggaggag atttggttca
      181 atcaggccga cggtttccat ccaagcgctc tgggtcccgc cacttatgcc gagctcctcg
      241 cccagtgcgg cagcgaggac cggttccggc tgaacgtgag ctacggcgac ggaacgccga
      301 tcgcgccgcc cgatctcgcc gccgtcaggc aggtcatccg ccgcgaaacg cgcccccata
      361 tctggcagtc gggcgacatt ctcatgctcg acaatctgct ggcggcccac ggccggcggc
      421 ccttttcggg agaacggcgg atcgccgtcg cgatgagctg agctggaagc ccgccggccc
      481 gccgttcgac ggccggggcc gggcttcgat cgctctgccg ttcatcaagc atttcagatt
      541 tcaggcttac cgaccgagta tcgccgggca gttccaccgg gccccggcgg cggagagacg
      601 ccatgctccg gccccgaagg agtgccgtgc aatgaccctc tacttcctgc acctcaggga
      661 tggcaccgac attgtcctcg acgaggaagg ctccgatcac gacagcctgg ccagcctttc
      721 ggcggagatg cttgcggcgg cgcgcgacgt catgcgcgaa gacgttcagc acgggcggct
      781 ggaccttcgc ctccgggtgg acgcggaaac ggccgacggc gcactggccc gaagcctgtc
      841 ctttgttgaa gcgctggaga tcgtcttccc ggagccggag agcgcatagc tcgcctcggc
      901 tagccgaggg ttggcaccgc ctggtccgcg gccgcgatgc aaggctgttg cggcgccgaa
      961 gcttccgctg tcgcaggacc ggtcccggca gtgccgccgc ggccggacgc gccgtagatg
     1021 cggtcgatcg ccgtggccgc ctcgctcagg ccgatagtgc tgccagacca gcgcgaaccc
     1081 tgggcgagcg actgcgccca gtgcgcggcg gcgcggccgc cccaggcatc cggcgggtcc
     1141 gccagccgct cacggcgggt tccgttcagc cgctcggcgg tgaaatcgta gaaggagcca
     1201 tcgacgttcc ggaaagcggc gccaccctcc gttccgtaga attcggcgcc gatgaccgcg
     1261 tcgctcccgg cggccaggtt ccacgagcag gcgacctgga ccgtcgctcc gtcttccagg
     1321 tcgagcgtgg ccgaggcgaa atcctcgatc ccaccggcct cgagcggccg tcctttcgaa
     1381 tagagccgcg aggaaacgcc ggtcacctgg ggaaaatcga gggtccacaa ggcgaggtcg
     1441 acgaggtgga tgccaagatc gatcacgcac ccgccgcccg acagttcgag gtcgtagaac
     1501 caggccttgt ccgggccgta ggcattgtgg aagacgaggt tggcggcgta gaccgtcccc
     1561 agcgcgcccg acccgatcag ctcgcgaacc gcgcgcatcc cctccgtata ccggtaggac
     1621 aggtcgactc cgagcaggcg gtcggcccgg cgggcggcgg cgagcacctg ttccacctcg
     1681 gcggcgttgc ggccgagcgg cttctggcag aagacggcgg cacccgcctc cagcacccgc
     1741 aggcactgcg ccgcgtggag ggcgctggga gtggcgatca ccactccgtc cggcgccagc
     1801 gccagcattt catccagcga ggtcaccaga gccgcgtccg gcgccagctt cagcgcctcc
     1861 gccgccatcg ccggattggg ctcgcagatc gccaccgcct cgaccccctc gatggcgagc
     1921 atcgcccgca tccggtcgag gccgatccag ccggtgccga tgaagcccag acgagtcatc
     1981 ggccgggtca cgggaaggtc accagcgcct tgacgaagcc cggcggccgg tcgcgggtgg
     2041 cgtccagcgc cgtgccgagc ccgtcgaggc cgtaaaggtg ggtgagcagg ggctcgggat
     2101 cgatccgccc cgccgccgcc gccgccgccg cctcgcgcat gcctcgaacg ctcaccgccg
     2161 ggtcgcgctc gtgggcgttg accacgtcga agccgcgcca gttccaaagc tgcatgttga
     2221 tctggcgtgg gccatcctga tgatagcccg cgactaccag acgtccgcgc tcgcgtgtca
     2281 attcggcggc aaggtcaagt ggccattgtt tgccgacagc ttcgatgacg caatcgcaga
     2341 acaggccgtt ggtcaacgca cgcacctgat cgatgatggc gtaatgatcg tccatggcga
     2401 tggtctcggc ggcgcccatc gccttcgcca gggcaagcga gcaaggcctg cgagagatgg
     2461 cgatcacatg ggcgccggca tcgctcgaca gccgggtcag gagcgctccg aggaagccga
     2521 tgccgacgat ggcgaccgtc tggccggcct cgatcccgct tcggcggaag atgttcatcg
     2581 cgcatccgaa cggctcgccc gggaaggcga ggccgtcgag ctcggacggc agctcgacga
     2641 ttccggcggc gtcggcgagg tcgtattcgg cataggcgcg gccgctcagc gccgccaccc
     2701 ggacgccttc ggcgatgccc tcgacgcctt cgcccacggc atcgatccgc ccccagccct
     2761 cgtggccgag ggttccgggc tcggtcggaa aggtcatcca gtcgggcccg gcccaaggtg
     2821 tcaggttgga cgcgcagacg ccgcagcctt ccagccggat ccgcacctgt cccggaccgg
     2881 gcgacggctt cgccacctcc tcgatcctca gccggccggg gccggtcagc accgcggccc
     2941 gcatcatttc tcttcctcga tggcgtcgag cacggcctcc ggcgcgacgt cgcgcttcag
     3001 ctcctggatt tcctcgtccg gcgccgggct gtcgactccg agccggtccg cgatggcgga
     3061 gaccagcgtt atcacccggg tgacctcgtg ctcggcgagg aggttgatct ggaggtcgag
     3121 agtggcccgg cgctcggctt ccgccgccat ccggttctgg ctgatcagga cgaaggtcga
     3181 caggaagatc gcctcgaccg aagcgatcat cgcaagaacg acgaaggtgg gatcccacgg
     3241 ctcgaagccg ggaatccacc ccagattggc gacgatccag ccgccgtaaa gggcgaggtg
     3301 cacatagacg aagcgcatcg agccggtgaa gcgggtgatc gcctccgcga gccgctcctg
     3361 gaacgtcgtc cggctccggt cgcgccgctc gcgctcgcgc aacgccgcga tgttgcgggc
     3421 gagcgctcgg ttgaggctgt cgggacccgg cggcgggacg gtcgggccct gactcattcg
     3481 gcggcctgga tcagccgggt ctccgcaaac cccggccgga ctcggccgat gccgtgccac
     3541 ggctccgccg tggcgccggc accggcgaac caggcgatgg tcgcttccag cccttcctgc
     3601 aggccgacgc gcggcgccca gccgagcagc tcggccgccc ggcctatgtc gggcttgcgg
     3661 cggcgcggat cgtcctcggg caggggccgg ttgacgatcg ccgagcgcga gccggtcatc
     3721 gccagcacgc ggtgcaccag atcctcgacg gtgagctcgt tgggattgcc cagattgacc
     3781 gggaagccga ccgccttctc gctttccatc aggcggagca gcccgtcgac gaggtcgctg
     3841 acatagcaga agctgcgcgt ttgcgagccg tcgccgtaga gggtgatgtc gtcgccggcg
     3901 agcgcctggc agatgacgtt ggagacgacc cggccgtcgt ccggccgcat gcgggggccg
     3961 taggtgttga aaatgcgggc gacccggacc tcgcaacgcc cgctcctttg ataatcgaag
     4021 gccagggtct cggcggcgcg cttgccttcg tcgtagcagg cgcgcgggcc cgtgcagctc
     4081 acccagccgc gataatcctc ggtctgagga tggacttcgg ggtcgccgta gacctcgctc
     4141 gtggaggtga gaagcaggcg cgcgccgttg acctcggcca gccggagcat gctttgcgtt
     4201 ccgacgacat tggtcatcat cgtgtgttcg ggatcggcct ggtaatgcgg cggcgaagcc
     4261 gcgcaggcga ggtggtagat gtgggtgaag gggatccggc cgccgcggat ccgccagggc
     4321 agcgggtcga tcacgtcgca ttcgaccagc tcgaaccggc tctcgccttc gagatggcgg
     4381 atgttctcgc gccggccggt gaggaagctg tcgagacaga cgacccgcgc gccctccgcg
     4441 atcagcgcgt cgcaaatgtg ggaaccgatg aacccggcgc caccggtgac taaggcgatc
     4501 ttctgcttgg gtttcttcac tacgggctcc ttcgggtggc tcggttcggt aagatagcgc
     4561 tcgagctcgg cggcgcgatg ggcggaatcg tgcgcgccga ggacccgctt gcgcgcggcc
     4621 gcgccgatcg cggcatcgtc cgccttcgcc gtgagcctgg cgacgacctc gtcgctgccg
     4681 tcg
//
LOCUS       DASWNU010000381         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_92975, whole genome shotgun sequence.
ACCESSION   DASWNU010000381 DASWNU010000000
VERSION     DASWNU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92975"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..2009)
                     /locus_tag="VGB57_07630"
     CDS             complement(216..2009)
                     /locus_tag="VGB57_07630"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY0131260.1"
                     /translation="MSTYEARLAALREQLKADRLDGFVVPLTDEHMSEYVGSYAQRLA
                     WLTGFQGSAGAAAVLPQEAAIFTDGRYTLQVRDQVDGRHWSYQSVPETSIADWLRAHA
                     PDGGRIGYDPWLHTKDWVARAKEALAEKGAELVPVQRNPIDAVWADKPEPSKARLVVH
                     PDDFTGKSSAAKRQEMADWLHEQKADAAVLSALDSIAWTFNVRGEDVEHTPVALSFAL
                     VNDDGTADLFVASEKIGDDVRQHLGNGVRVHERHAFEPHLRSLAGKTVVADPERAVHA
                     IFEALEKAGARIVQKRDPAVLPKAIKNRAEIEGHRAAQARDGAALSRFLHWLSIEAPK
                     GNVDELIAVEKLQSFRQACGDLRDTSFDTISGAGPNGAIVHYRVSEETNRKLEMNSIY
                     LVDSGGQYPDGTTDVTRTIAVGEPTAEMRDRFTRVLKGHIAVARAVFPEGTRGSQLDS
                     FARQYLWQAGLDYAHGTGHGVGAYLAVHEGPQRISPAGSSQSGGDEPLRAGMFLSNEP
                     GYYKTGEYGIRVENLVLVEPREVEGAEKRMLGFETLTFAPIDRKLVEAGMLSAEERDW
                     LNAYHAKVLEVVGPQLEGEAKAWLEAGCAPL"
     gene            2077..>2766
                     /locus_tag="VGB57_07635"
     CDS             2077..>2766
                     /locus_tag="VGB57_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0131261.1"
                     /translation="MTLPAPPRAEPRPYSYERHGYTIEDPYAWLKDPGYPTVEDEEIL
                     AYLKAENAYFEAAMAPHSGLVETLFEEMKGRIEEDESSVPVRERDWLHWWAYRPGMQY
                     RTWYRRPVSGGGEQVIFDEPVEAEGKEYFRAGPIEFSPDGRLAATLVDDSGSERFHLR
                     IRDLATGKDIETVTDVAIGNAVWTSDSGGVVFTEVNENWRSYRARLHRLGEPVERDVT
                     LYEETEDKGFSV"
BASE COUNT          441 a          901 c          978 g          446 t
ORIGIN      
        1 tatggccgga cgggcggtgg gatgtcaagg taagtcatgg cgaagcggga cgaagagcgg
       61 gagaaacggc tggccgaggc gcttcgcgag aatctgcggc gccggaaggc gcaggcgcgc
      121 ggcgaggggg aacgtcatcc cggcgaaagc cgggacctcg gggaggccca ccgagacttc
      181 tcgtcacgag gtcccggctt tcgccgggat gacggctaaa gcggcgcgca gcccgcctcg
      241 agccaagcct tggcctcgcc ctcgagttgc ggcccgacca cctccagcac cttcgcatga
      301 taggcgttga gccagtcccg ctcctcggct gagagcatcc ccgcctcgac cagcttgcgg
      361 tcgatcggcg cgaaggtgag ggtctcgaag cccagcatcc tcttctccgc cccctcgacc
      421 tcgcggggct cgacgagcac cagattctcg acccgaatgc cgtattcgcc ggtcttgtaa
      481 tagcccggct cgttggacag gaacattccg gccctgagcg gctcgtcgcc gccggactgg
      541 ctcgatccgg ccggggagat gcgctgcggc ccctcgtgga cggcgaggta ggcgccgacg
      601 ccgtggccgg tgccgtgggc gtagtcgagg ccggcctgcc acagatactg gcgggcgaag
      661 ctgtcgagct ggcttccgcg cgtgccctcc gggaagacgg cccgggcgac ggcgatatgg
      721 cccttgagaa cccgggtgaa gcggtcgcgc atctccgccg tcggctcgcc gaccgcgatg
      781 gtgcgggtca cgtcggtggt tccgtcggga tattggccgc ccgaatcgac cagatagatg
      841 gagttcatct cgagcttgcg gttggtctcc tcgctcaccc gatagtggac gatggcgccg
      901 ttcgggccgg cgccggagat ggtgtcgaag ctggtgtcgc gaaggtcgcc gcaggcctgg
      961 cggaatgatt ggagcttctc gaccgcgatc agctcgtcca cattcccttt cggagcctcg
     1021 atcgaaagcc agtggaggaa ccggctcagg gcggcgccgt cgcgcgcctg ggcggcgcgg
     1081 tggccctcga tctcggcccg gttcttgatc gccttgggca ggacggcggg atcgcgcttc
     1141 tggacgatcc tggcccccgc tttctcgagc gcctcgaaga tggcgtgaac cgcccgctcc
     1201 ggatcggcga ccaccgtctt gccggcgagc gagcgcaggt gcggctcgaa ggcgtggcgc
     1261 tcgtggaccc gcactccgtt gccgagatgc tgccgaacgt cgtcgccgat cttctccgag
     1321 gcgacgaaaa ggtcggcggt gccgtcgtcg ttgaccagag cgaaggagag ggcgaccggc
     1381 gtgtgctcga cgtcctcgcc ccgcacgttg aaggtccagg cgatcgaatc gagcgccgag
     1441 agcacggccg cgtcggcctt ctgctcatga agccagtccg ccatctcctg gcgcttggcg
     1501 gcggaggatt tgccggtgaa atcgtcggga tggacgacca gccgcgcctt ggagggctcg
     1561 ggcttgtcgg cccagacggc gtcgatcggg ttgcgctgga ccgggaccag ctcggcgccc
     1621 ttctcggcca gcgcctcctt tgcccgggcc acccagtcct tggtgtggag ccagggatcg
     1681 tagccgatcc ggccgccgtc gggggcgtgc gcccttagcc agtcggcgat gctggtctcg
     1741 ggcaccgact ggtagctcca gtgccggccg tcgacctggt cgcggacctg gagcgtgtag
     1801 cgcccgtcgg tgaagatcgc cgcttcttgc ggaagcaccg ccgcggcgcc ggccgagccc
     1861 tggaagccgg tcagccaggc gaggcgctgg gcgtagctcc cgacatattc actcatatgt
     1921 tcgtccgtga gcggaacgac gaagccgtcg aggcggtccg ccttgagctg ctcgcgaagg
     1981 gcggcgagac gggcttcgta ggtcgacatg gcaaaatcct caggcgtcgc tattgggctg
     2041 tccgatagac cagcacgccg cgccacgaaa gagagcatga cccttccagc cccgccccgc
     2101 gccgaaccgc gcccttacag ttacgaacgc cacggctaca cgatcgagga tccttatgcc
     2161 tggctcaagg acccgggata tccgacggtc gaggacgagg agatcctcgc ctatctcaaa
     2221 gccgagaacg cctatttcga ggcggcgatg gcaccccatt ccggcctcgt cgaaaccttg
     2281 ttcgaggaga tgaagggccg gatcgaggag gacgaatcct cggtcccggt gcgggagcgc
     2341 gactggctcc attggtgggc atacaggccg gggatgcagt atcggacctg gtaccggagg
     2401 ccggtctcgg gcggcgggga gcaggtcatc ttcgacgagc cggtagaggc ggagggcaag
     2461 gaatatttcc gggccgggcc gatcgagttc agccccgacg gccgactcgc cgccaccctc
     2521 gtcgacgaca gcggctcgga gcggttccac cttcgaatcc gcgacctcgc gacgggcaag
     2581 gatatcgaga ccgtcaccga cgtcgcgatc ggcaacgcgg tctggaccag cgacagcggc
     2641 ggcgtggtct tcaccgaggt gaacgagaat tggcgcagct accgcgcccg tctgcaccgg
     2701 cttggcgagc ccgtcgagcg cgacgtcacg ctgtacgagg agaccgagga taagggcttc
     2761 tcggtc
//
LOCUS       DASWNU010000382        10326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_92976, whole genome shotgun sequence.
ACCESSION   DASWNU010000382 DASWNU010000000
VERSION     DASWNU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92976"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VGB57_07640"
     CDS             complement(<1..681)
                     /locus_tag="VGB57_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEY0131262.1"
                     /translation="MIVLLGFWTHALAALLYGALALWQLRRWNEDGGNRRLVAAFAVT
                     SAWAIFVALQGPHSLGAGIAEAARNFAFLGFMYAIVSRAADERVQQGVKLVYTVVAGV
                     IGLQLTLAGVTPQYAGTVVLHQALVSTSQVIGLTIAAGSLVLVHNLYGQAAHESRWGI
                     RLPMIALAGMWAYDLHLYTVAWLTRGPVEDLYAMRGAILSMLVPLFALASRRNSAWRM
                     QLSRAATFQ"
     gene            complement(678..2066)
                     /locus_tag="VGB57_07645"
     CDS             complement(678..2066)
                     /locus_tag="VGB57_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEY0131263.1"
                     /translation="MIRLFKHYVPHSVLMLGVIDFLLLFAAGEAGWLLRLWQIEGGVD
                     PNAQRLPHLLTFAIVLQLAMVGVGAYGVDALRSMRLAAARLVVSVSLGVLLLALVFFL
                     FPALTFWRSNLLYAMGFALVLLFIARLVFGRALGNEAFKRRILVLGAGPRAARLEALA
                     RTEGAGFAIAGYVGMNDGPQAVPHALNRDDIANLTAHVVRLRVAEVVLALEERRNALP
                     LADLLRIKTTGVHVNEFSSFIERETGRVDLDSVNPSWLIFSDGFSSGRRLSAAGKRMF
                     DIVVSGLILILFAPLILLTALAVKLESKGPAFFRQRRVGLYGQSFELVKLRSMREDAE
                     VGGKAVWAQKDDPRVTRVGSVIRKLRIDELPQAWTVLTGKMSLVGPRPERPQFVADLE
                     ARLPYYAERHVVKPGITGWAQINFPYGASVEDARQKLEYDLYYAKNYTPFLDLLILLQ
                     TVRVVLFPEGAR"
     gene            2581..3296
                     /locus_tag="VGB57_07650"
                     /pseudo
     CDS             2581..3296
                     /locus_tag="VGB57_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611329.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            3512..4147
                     /locus_tag="VGB57_07655"
     CDS             3512..4147
                     /locus_tag="VGB57_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0131264.1"
                     /translation="MRLYTRPIAPNALKVLIFMTEGGIEIETVDVGDLAPEDYARVSA
                     LRTVPVLETDGGLFITESLTICQYLDVSLPGPSLFGEGLEERTVVAMWERRAELMLMN
                     PAIEYGHHTQPMFAGRLAQFPDWARAHVAQSRKMLELMEARLAEVRYLAGERFTMADL
                     TAFLGWAGLVGWRAIEPRPGPALGRWLGEVGARPSMAPLRALAAQFDLPSV"
     gene            complement(4788..5384)
                     /locus_tag="VGB57_07660"
     CDS             complement(4788..5384)
                     /locus_tag="VGB57_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEY0131265.1"
                     /translation="MCNLYRQRSTENLGRLFDAVALDAPFEARPDIYPRYEALVVRKE
                     GGTRRLDCMTWGIVRTMPGKSGKPVKKAITTLRNLESPFWRSTLADPARRCLVPFTSF
                     AEPRPGKDPETGRPANWWFTVEGEEGCAFAGIWRMSEGSKRFAFLTCEPNPLVAPLHP
                     KAMPVILQREDYDAWLDGEAADACSLAQPFPSQLMRVA"
     gene            5482..5790
                     /locus_tag="VGB57_07665"
     CDS             5482..5790
                     /locus_tag="VGB57_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131266.1"
                     /translation="MDKRIAVTPSELARAIGVWLRVMPKPVWRALEKYQLAALEKRHD
                     PDREPPVHAAVADHIAAHFQRANWEITRPEPKDLGSPPPWRGMTDAGTTDARDDGTKP
                     "
     gene            6262..6906
                     /locus_tag="VGB57_07670"
     CDS             6262..6906
                     /locus_tag="VGB57_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0131267.1"
                     /translation="MAGRTLAARIGTGLLAGAALLVVGFEVADEPARAPAAAEAPAAP
                     TAPVIAASAPAPAAPHFTVRRILDSPGPLKMGEHHWDESGAPADGPIVITVDLEAQVL
                     SIFRGGYEIGASAILYGRDDKPTPLGAFPITQKKAKHVSNLYGSLMPYMMRMNDDGIS
                     IHGSDSVAPGFATHGCVAVPVAFAKKLFGAAKLGDVVIVTRGKRLEMGEAIVRT"
     gene            7169..8128
                     /locus_tag="VGB57_07675"
     CDS             7169..8128
                     /locus_tag="VGB57_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0131268.1"
                     /translation="MTERRPIGRSGLETPRLVLGGNVFDWTADERTSFALLDAFVDAG
                     GTMIDTADVYSAWVPGHQGGESETLIGEWLRRRGSKAGVAIASKVGMLHRHGGGKLEP
                     WRIEKAAEASLKRLGIETIDLYYAHQDDPDTPMEDVIEAFDRLVRAGKVRTLGASNFT
                     AERLGRALSLAREMGTSRYEVLQNEYHLLERGKLEGELQRLCLAETIGILPFYGLASG
                     YLTGKYRSESDYGKSIRGDRMAKYLEGRGPAILSLLDEIAAETGATQGQIALAWVAAQ
                     PAVAAPIASARTVDQLEELLGAMELVLSPAQLARLDEVSAATV"
     gene            8314..8493
                     /locus_tag="VGB57_07680"
     CDS             8314..8493
                     /locus_tag="VGB57_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131269.1"
                     /translation="MDSKLSEASSSKHKIEGLHDRLNDLLESLDKAELHGAAAYVSMA
                     LDSIQRDYPEIRSTR"
     gene            complement(8477..8950)
                     /locus_tag="VGB57_07685"
     CDS             complement(8477..8950)
                     /locus_tag="VGB57_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131270.1"
                     /translation="MMVAQPTGRHGPEDASHLATETERIILEIESRLHRLIETSRRES
                     GPAAQSSVLAAAAEACAARRRLYALFGRRLFCDPSWDILLELFVSTLEGRKVTVSTAC
                     MAACAPTTTALRHIAYLVQEALVVRRPHPADARSTYLELTPQAITRLTQYFNEST"
     gene            complement(8947..9573)
                     /locus_tag="VGB57_07690"
     CDS             complement(8947..9573)
                     /locus_tag="VGB57_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HEY0131271.1"
                     /translation="MIDLVLPESRLGFAAALMEMHHDRKQVFVDRFGWRLPAKGSWLE
                     VDQFDDDYAIYLLARSPEGRHQGSVRLLPSSRPHMLSTLFSHLCPAGVPSGDDCWEIS
                     RLVTCPADASGTTILRVHRLLALALVEFAQSNGIERYTLVAEAHRVPALLSVGWPVTP
                     LGLPTWFEGEQLQALQIDLPPGTLHEMRSRLRIEAPVLRIVEPRRRAA"
     gene            9691..>10326
                     /locus_tag="VGB57_07695"
     CDS             9691..>10326
                     /locus_tag="VGB57_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0131272.1"
                     /translation="MTAPFLPPIAYREKPPAIRREFPRVRGRDLESVPGKRGLPFFGI
                     LPEAVLDPLAFARRMHETYGPVHRFHACGNWNVQLVGPEANEFVLFDEARNFSAEGGW
                     RPVFGRHFEGGLLLRDGANHQWHRKLVATAFKQEQLQSYLDIFAGNNAKVLAEWSGRS
                     LDVYELAQQLSFANGYSAFLGRDPELATRTDMLAFRYLMRAAIAVVTLPLPG"
BASE COUNT         1818 a         3379 c         3481 g         1648 t
ORIGIN      
        1 ctggaaggtg gccgcccgcg acaattgcat ccgccacgcc gagttgcggc ggctggcgag
       61 cgcgaacagg ggcacgagca tcgacaggat cgcgccgcgc atggcgtaga gatcctcgac
      121 cgggccgcgg gtcagccagg cgacggtgta gaggtggagg tcgtaggccc acattccggc
      181 cagggcgatc atcggcagcc ggattcccca gcggctctcg tgcgccgcct ggccgtagag
      241 attgtggacg aggacgagcg atccggcggc gatggtgagg ccgatcacct gcgaggtgga
      301 gacgagcgcc tggtgaagca cgacggtgcc ggcatattgc ggagtcaccc ccgccagggt
      361 gagctggagc ccgatcacac cggcgacgac ggtgtagacg agcttgacgc cttgctgcac
      421 gcgttcgtcg gccgcccggc tgacgatcgc gtacatgaag ccgaggaaag cgaagttgcg
      481 ggccgcctcg gcgatcccgg ctccgaggct gtgcggcccc tgcagggcga cgaagatcgc
      541 ccaggccgag gtcaccgcga aagccgcgac caggcggcgg ttgccgccgt cctcgttcca
      601 ccgccgaagc tgccacaagg ccagcgcccc gtagagcaaa gcggcgagcg cgtgggtcca
      661 gaatccgagc aggacgatca tcgcgcgccc tccggaaaca gcaccacccg caccgtctgg
      721 aggaggatca ggaggtccag gaacggggtg tagttcttgg catagtataa atcgtattcg
      781 agcttctgcc tcgcgtcctc gacgctggcg ccgtagggga agttgatctg cgcccagccg
      841 gtgatcccgg gcttcacgac gtggcgctcg gcataataag gaagacgcgc ctcgaggtcg
      901 gcgacgaact ggggacgttc gggccgcggg cccacgaggc tcatcttgcc ggtcagcacc
      961 gtccaggcct gcggcagctc gtcgatcctc agctttcgga tcaccgagcc gacccgggtc
     1021 accctgggat cgtccttctg ggcccagacc gccttgccgc cgacctccgc gtcctcgcgc
     1081 atcgagcgca atttcaccag ctcgaagctc tgcccgtaaa ggccgacccg gcgctgacgg
     1141 aagaaggccg gccccttgct ctccagctta acggcgagcg cggtcaggag gatgagcggg
     1201 gcgaagagga tcaggatcag gcccgagacg acgatgtcga acatcctctt gcccgccgcc
     1261 gacagccgcc gccccgacga gaatccgtcc gagaagatca gccagctggg attgacgctg
     1321 tcgagatcga cccgccccgt ttcacgctcg atgaaggagg agaattcgtt gacatggact
     1381 ccggtcgtct tgatcctcag caggtcggcg agcggcaagg cgttgcgcct ctcctccagc
     1441 gccagcacca cctcggccac tcgaagcctg acgacatggg cggtgagatt ggcgatgtcg
     1501 tcgcggttca gggcgtgcgg caccgcctgt gggccgtcgt tcatcccgac atagccggcg
     1561 atcgcgaagc ccgccccttc cgtccgcgcc agcgcctcga gccgggccgc gcgcggcccg
     1621 gcgccgagga cgaggatgcg ccgcttgaag gcctcgttgc cgagcgcccg gccgaagacg
     1681 aggcgggcga tgaacagcag gaccagcgcg aagcccatcg cgtagagcag gttcgagcgc
     1741 cagaaggtca gcgccggaaa gagaaagaag accagggcga gcagaagcac tccgagcgag
     1801 accgacacca ccagccgggc cgccgccagc cgcatcgagc gaagcgcgtc gaccccgtag
     1861 gcgccgaccc cgaccatggc gagctggagc acgatcgcga aggtgagcag atgcggaagc
     1921 cgctgcgcgt tgggatcgac gccgccctcg atctgccaca ggcgcaacag ccaccccgcc
     1981 tccccggccg cgaacagcag caggaagtcg atcacgccca gcatcaggac gctgtggggg
     2041 acgtaatgct tgaacaacct gatcaagggg cgagcgcctc gcggagtgta agttctgccg
     2101 acagggttct agtgtcgctc aaattgacaa agaaagcgtg aaaagcgtcg gattggagtg
     2161 aggaggggcg cgcggagggc ttccgggaag ggcctcccgg gcgcgtggcc cgaatgcgaa
     2221 atgaccgatc gtgcggacgt cgaaccgcgc agccgacctg tccctcgcaa cgaaaaacga
     2281 cgaagcgaag gtcccggcgc tccaggcggt gccgcggagt acagccggcg atacgcggga
     2341 ttcggcgcgc acggtcgatc gggctgccat tgaagagccg accaggctcc aattgggtga
     2401 cagtcactag tgacagtcac tagtgactgt cactagggcc aatcggtgac agtcactagt
     2461 gacagtcacc gccccccagc gcctgcttga aaagtgacag tcactagggt ccggagccat
     2521 taacgggatt ccctttcgcg gtgggtcgtg gttcactgcc tttgagagga gggcggtgag
     2581 atgtctgact tcttttggtt ttcggatgcg caatgggcga ggatcgagcc gctgctgccg
     2641 accgacgtgc gcggcatgaa gcgcgtcgat gaccggcggg ttctgagcgg catcgtccat
     2701 gcgctcaagt ccggcgggcg ctgggcggat tgcgccgaca ttcatagtcc cgggaagacg
     2761 ctctacaacc ggttcgtgcg ctgggctgag cgcggcgtct gggagaggat cttcggcgcg
     2821 ctcgccagcg ccgaggaagc cgatcgattg ttcatcgatt ccagctgcat caaggcccac
     2881 cgctgcgccg gcggggcaaa agggggggcc tggctcatgg tatcggccgc accaaaggcg
     2941 gccgcaatac caagctccac gccgtctgcg acgccaaggg ccgccctctc gtcctcgtcc
     3001 tgacgcccgg caacacccac gactgcaagg tcgcccagct ctgcatagag gcgctgccgc
     3061 cttcggccgg gctcgtcgcc gacaagggct atgacagccg gctcttgcgc gaatggctcg
     3121 aagcgcgagg caccaccccg gtcatcccgc cgcgaagcta ccgcaaggtc caataggcct
     3181 gcgatcgcgc tgcctacagg caccgcaaca tcatcgagcg catgttctgc cgtctcaagg
     3241 actggcgacg catcgccacc cgctacgacc gaaagctcag caccttcgga ccctagtgac
     3301 tgtcactttt cgaggcgggc ctagtgggcc tagtgacagt cactagtgac agtcactttc
     3361 gacgaggttg cggtgacggc gtgcctagtg acagtcactt ttgagccttg agtgactgtc
     3421 actcacgccc gaagccaggc ccgttaccgc gactttgcac ctgttcgcgg acggcggccg
     3481 gccgtaccac tcccccaggg gaggaccgcc aatgcgcctg tacacacgcc cgatcgctcc
     3541 gaacgcgctc aaggtactca tcttcatgac ggaaggcggg atcgagatcg agacggtgga
     3601 tgtcggcgat ctggcgccgg aggattatgc ccgggtcagc gcgcttcgca ccgtgccggt
     3661 gctggagacg gacggcggcc tcttcatcac cgaatccctg accatctgcc agtatctcga
     3721 cgtctcgttg ccggggcctt cgctgttcgg cgagggcctt gaggagcgca ccgtcgtcgc
     3781 gatgtgggag cggcgggcgg agctgatgct gatgaacccg gcgatcgaat atgggcacca
     3841 tacccagccg atgttcgccg gccggctggc gcagtttccg gactgggcgc gggcgcatgt
     3901 cgcccagagc cggaagatgc tggagttgat ggaggcgcgg ctggcggagg tccgctacct
     3961 cgccggcgag cgcttcacca tggccgatct gaccgccttc ctgggctggg ccggcctggt
     4021 cggctggcgg gcgatcgagc ctcggcccgg gccagcgctc ggccgctggc tcggcgaggt
     4081 cggcgcccgg ccgagcatgg cgccgctccg cgcgctcgcc gcgcagttcg atctcccctc
     4141 ggtgtgaacg agcgcgagag cccggccctt cgaagctggt cgttgccggc gaagccggtg
     4201 gagcggttcc ccgggcgccc cggcgaaacc tttccaccgg cattcgcgcg gcagctcctt
     4261 ccttcgaacg aagcgaaaga aagttcattt ccgttcacga tgtcaaagag ccctgccagt
     4321 ccgcgactcg gcatgggaac aatataggaa cggcaagagc cgaaatctcc ttcacttaaa
     4381 tccgtttcgg aagttgcaga gccgatgcgg cgcccgggac gtgcgggacc aggcaccccg
     4441 atgcggattt gggatacaat tcggcctgcc ctattccagt cttgcggagg gcgggaatga
     4501 gcaggtggcg ccgtggccga tcgtatggat cgttttcggc tttggcatta tgatgttcgc
     4561 cacccgattg ttgggcgatt tggaggcgga gagctgaaat gaccttggcc ggtaatattg
     4621 cccccgtcgt cggtggcgtc ctgttctgcg ccatcgctta tgtctcggta cgggcggcgc
     4681 atcggaaagg ccgggatgca cctgtgcccg agcgaaaggc cgagcccgcc gagtaatccc
     4741 cgccgccttc gccgccgcaa agcgattcga ccttggtcga actcgctcta ggccacccgc
     4801 atcagctggc tcgggaaggg ttgggccagc gagcaggcgt cggccgcctc gccatccagc
     4861 caggcgtcgt aatcctcgcg ctggaggatg accggcatcg ccttggggtg gagcggggcg
     4921 acgagggggt tgggttcgca ggtgaggaag gcgaagcgtt tggagccctc gctcatccgc
     4981 cagatgccgg cgaaggcgca gccttcctcc ccttcgaccg tgaaccacca gttcgccgga
     5041 cggccggtct cgggatcctt gccggggcgg ggctcggcga agctggtgaa ggggacgagg
     5101 cagcgccggg cgggatcggc gagggtcgag cgccagaagg ggctctcgag gttgcggagg
     5161 gtggtgatcg cctttttgac cggcttgccg ctcttgcccg gcatcgtccg gacgatgccc
     5221 caggtcatgc agtcgagccg gcgcgtcccg ccctccttgc ggaccaccag ggcctcatat
     5281 ctgggataga tgtccggcct ggcttcgaag ggggcatcga gcgcaacggc gtcgaacagg
     5341 cggcccaggt tctcggtgct gcgctggcgg tagagattgc acatcaggga ggattaactt
     5401 ccccggcgac gaaggggaag cgtcgccgca tatgcaaaaa tcgattgcgc tccctagaga
     5461 ttgttcccta tttgttctcg catggacaag cgaatcgccg tcaccccttc cgagctcgcg
     5521 cgggcgatcg gagtctggct tcgggtcatg cccaagcctg tctggcgggc gctcgaaaaa
     5581 tatcagctcg cggcgctcga gaagcggcac gatcccgacc gggaaccgcc ggtccacgcc
     5641 gccgtcgccg atcatatcgc cgcccacttc cagagggcca attgggagat cacccggccc
     5701 gagccgaagg acctgggctc gcctcccccc tggcgcggga tgacggatgc cgggacgacg
     5761 gatgcgcggg atgacgggac gaagccgtaa gggggccgag cggcccgtgg aagcaaaccc
     5821 cccggccccc accgtgagag gaagccattc caaactctcg gtaaacctcc gatatcatac
     5881 tgatcgaggt taaagataga tgtaagttag ttggcctcgg caaaattttc gagcgattac
     5941 aacggggagg cgcatttctc ccgattcctg tcaggccgct tgcgaaacat ccacattcgg
     6001 atcaaccttc cttgccggcg ctttcaccaa agcgattcgg cgcgggcccg gcccgccacc
     6061 tggacatccg gcgctgcgcg gggggcgacg cggcgggagt cttcagggcg gccgggcccg
     6121 cttgcagccc cgggccccta acatttgccg gcccgccgtc ccgcgccggc acaggccgca
     6181 tcgggggctg gaagggcggg atcgcttgcg acattggccg cttttacagg gcctggacag
     6241 gtttcacgaa aggcgaaggc gatggcggga cggactttgg cggcgaggat cgggacgggc
     6301 ctgctggccg gcgcggcact gctggtcgtc ggcttcgagg tcgccgacga gcccgcccgg
     6361 gccccggcgg cggcggaggc ccccgcggcc cccaccgccc cggtcatcgc cgcctcggcg
     6421 ccggcgcccg ccgctcccca cttcacggtc cgtcggatcc tcgacagtcc cggccccctc
     6481 aagatgggcg agcatcattg ggacgagagc ggcgcccccg ccgacgggcc gatcgtgatc
     6541 accgtcgatc tcgaggcgca ggtcctctcc atcttccgcg gcggctatga gatcggcgcc
     6601 tccgcgatcc tctacgggcg cgacgacaag ccgactccgc tcggcgcctt cccgatcacc
     6661 cagaagaagg cgaagcacgt ctccaatctc tacggctcgc tcatgcccta catgatgcgg
     6721 atgaacgacg acggcatctc gatccacggc agcgacagcg ttgcccccgg cttcgccacc
     6781 cacggctgcg tcgccgtgcc ggtcgctttc gcgaagaagc tgttcggagc ggcgaagctc
     6841 ggcgacgtcg tcatcgtcac ccgcggcaag cggctggaga tgggcgaggc gatcgtccgg
     6901 acttgaaagc ggggatagtc gcgagcccgt catgctgagc tcgtttcagc atccagcgac
     6961 gagccgcctc gcgggcggag aactggaccc tgaaacgagt tcagggtgac gaagaactgg
     7021 cggtccgtcc gacctctgct acaccgccct cgatgagcgc cctggaatcg gcccgtcatc
     7081 ctttccggcg gcgccgggac cggctctggc cgctgtcgcg ctggaggcga ggcgccggcg
     7141 acggagggcg ggatgaagga ggacagtcat gactgaacgg cgacccatcg ggcgcagcgg
     7201 gctcgagacc ccgcgcctgg ttctgggcgg caacgtcttc gattggaccg ccgacgagcg
     7261 gacgagcttc gccttgctcg acgcgttcgt cgacgccggc gggacgatga tcgacaccgc
     7321 cgacgtctat tctgcctggg tgccggggca tcagggcggc gaatccgaga ctttgatcgg
     7381 cgagtggctg cggcggcggg gatcgaaagc gggcgtggcg atcgccagca aggtgggaat
     7441 gctccaccgc cacggcggcg gcaagctcga gccctggcga atcgaaaagg cggcggaagc
     7501 gtcgctgaag cgtctcggca tcgagacgat cgacctttat tacgcgcacc aggacgatcc
     7561 cgacacgccg atggaggacg tcatcgaggc gttcgaccgg ctggtcaggg ccggcaaggt
     7621 gcgcacgctc ggcgcctcca acttcaccgc ggagcgactc ggacgggcgc tgtcgctggc
     7681 tcgggaaatg ggaacgagcc gctacgaggt gctccagaac gaatatcatc tcctggagcg
     7741 cggcaagctc gagggcgagc tgcaacggct ctgcctcgcc gagactatcg ggatattgcc
     7801 cttctacggc ctcgcctcgg gctacctcac cggcaaatat cgaagcgaat cggattacgg
     7861 caaaagcatc cgcggcgacc ggatggcgaa atatctcgag ggccgcggcc cggcaattct
     7921 ctccctcctc gacgagatcg cggcggaaac cggggctacc cagggccaga tcgccctcgc
     7981 ctgggtcgcc gcccagccgg ccgtcgccgc ccccatcgcc agcgcgagga cagtcgatca
     8041 gctggaggag ctgctcggcg cgatggagct cgttctgtct cccgcccagc tggcgcggct
     8101 ggacgaagtg tcggcggcga ctgtctaaaa tcggaaactt tcgttcaaaa cggatcgact
     8161 agttgcgccg cccccctttt cgagcggctt acgttagcgt aaaggaggcg caggggcttc
     8221 tggcctggac gcgtgatggg acgcgtttcg ggcgaagggt gcgctgccag cgacgatctg
     8281 gaacagctgc gcaccgaaag gggaggccca tggatggact cgaagctatc ggaagcgagt
     8341 tcctcgaagc acaagatcga gggcctgcac gacaggctca acgatttgct cgagtcgctc
     8401 gacaaagcgg agctgcatgg cgcggccgcc tacgtctcga tggcgctcga ttcgatccag
     8461 cgcgactatc ccgagatcag gtcgactcgt tgaaatattg ggtcaggcgc gtgatcgcct
     8521 gcggcgtcag ctcgagatag gtgctgcgcg cgtcggccgg gtggggacgg cgtacgacca
     8581 gcgcttcctg aacgagatag gcgatgtggc gaagcgccgt cgtggtcgga gcgcaggccg
     8641 ccatgcacgc ggtcgaaacg gtgaccttgc ggccctccag cgtgctcacg aagagctcga
     8701 gcaatatgtc ccaggaggga tcgcagaaga gcctccgccc gaacagggcg tagagcctgc
     8761 ggcgagcggc gcaggcttcg gccgccgccg ccagcaccga ggattgcgcg gccggacccg
     8821 attcgcggcg cgaggtctcg atcagccggt gaaggcgcga ctcgatctcg agaatgatcc
     8881 gctcggtttc ggtggcgaga tggctcgcat cttccggccc atgcctgcct gtcggctgtg
     8941 cgaccatcac gcagctctcc gcctcggttc cactatgcgc agcaccggag cctctatccg
     9001 taagcggctg cgcatttcgt gcagtgtgcc cggcggcagg tcgatctgca aggcctgaag
     9061 ctgctcgcct tcaaaccagg tcggcaaccc cagcggagtc accggccagc ccaccgaaag
     9121 cagggccggg acccggtgcg cctcggcgac gagcgtatag cgctcgatgc cgttggactg
     9181 ggcgaactcg accagggcca aagcgaggag gcggtgcact cgaaggatgg tggtgccgga
     9241 ggcgtccgct gggcaggtca cgagacgcga tatctcccag cagtcgtcgc cgctcggaac
     9301 cccggccgga cagagatgac tgaagagagt gctcagcatg tgcgggcgtg aggacgggag
     9361 aagcctcact gacccctgat ggcggccttc cggcgatctg gcgagcaggt agatcgcata
     9421 atcatcgtcg aactgatcga cctccagcca gctacccttt gccggcagac gccatccgaa
     9481 ccgatctacg aacacctgct tccggtcatg gtgcatctcc atgagcgccg cggcaaagcc
     9541 gagacgactt tccggaagaa ccaaatcaat catgcttcag cctccgacgc cctctaagtc
     9601 cgccatcggc acgtcgaatc acaccccggt agttaccggg taggggcgcg ggtcccggcg
     9661 cgtgtaggtt ccccggccac aggagccctc gtgaccgcgc ctttcttgcc acccatcgcc
     9721 tatcgcgaga agccgccggc gatccgccgc gagttcccgc gcgtgcgcgg ccgggatctc
     9781 gagtcggttc cgggcaagcg cggcctgccc ttcttcggaa tcctgcccga ggcggtcctc
     9841 gacccgctcg ccttcgcccg gcggatgcac gagacctacg ggccggtcca ccgcttccac
     9901 gcctgcggca actggaacgt ccagctggtg gggccggagg cgaacgagtt cgtgctcttc
     9961 gacgaggccc gcaatttctc cgcggaaggg ggatggaggc ccgtcttcgg ccgccatttc
    10021 gagggcggcc tgctgcttcg cgacggcgcc aaccaccaat ggcaccggaa gctcgtcgcc
    10081 accgccttca agcaggagca gctgcaatcc tatctcgaca tcttcgccgg caacaacgcg
    10141 aaggtgctgg cggaatggtc cgggcggtcg ctcgacgtct acgagctcgc ccagcagctc
    10201 agcttcgcca acggctactc ggctttcctc ggacgcgatc ccgagctcgc gacgcgcacg
    10261 gacatgctcg ccttccgcta tttgatgagg gccgccatcg cggtcgtcac cctgcctctg
    10321 cccggt
//
LOCUS       DASWNU010000383         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_482634, whole genome shotgun sequence.
ACCESSION   DASWNU010000383 DASWNU010000000
VERSION     DASWNU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482634"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1726
                     /locus_tag="VGB57_07700"
     CDS             <1..>1726
                     /locus_tag="VGB57_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0131273.1"
                     /translation="GGNGIYGGDGDDRIDLTGAGYGPSAYGEAGDDYIIGGNSYERLD
                     GGEGNDRIEGNYGYDNLYGGAGNDIIDGGSFDDNLYGEAGNDTLYGAGDVDTIDGGDG
                     DDIIYGDFSLISSAGGISGGPRFETLRGGAGDDFVSGDEGDDRLYGDDGNDRLYGGTG
                     NDSLTGGAGADRMEGGEGNDTGYGGDGEDVILAGTGTDQLSGEGDNDRLYFGADFGGS
                     DLAAGGAGLDQLVLQGNYAALTLAAANIVSVETIRLLSAGDSSIVTAPGGSFSYELTA
                     ADSLVSGSNVLRVEAPGLAAGENLRFDGSAETSASFAVVGGAGADLIRTGAGNDSLEG
                     GGGDDTLLGGAGDDLLTGGDGNDSLNGEAGNDTLTGGAGADNLIGGDGNDVYILDSIA
                     HAVIEHPNEGVDEVRIGVPGYVLPANVENLTYTGGFETNLVANAGNNVVTGGDAREVY
                     YMQQGGDDIVRSFGGNDTFVFGATFNAADSIEAGAGTDGLILQGNYPALTFGTGTTSN
                     ISGVETISLAPGHFTDWGDTSGSFYDYNVTTLNGNVAAGAVLKFNGFYLRAGEDFTFN
                     GAAETDGKF"
BASE COUNT          316 a          553 c          591 g          266 t
ORIGIN      
        1 ggcggcaacg gcatttacgg cggcgacggc gacgatcgga tcgatcttac cggcgcgggc
       61 tatgggccaa gcgcctatgg ggaggcgggc gacgactata tcatcggcgg caacagctac
      121 gaacggctcg acggcggcga gggcaacgac cggatcgaag gcaattacgg ctacgataat
      181 ctttacggcg gagccggcaa cgacatcatc gacggcggca gcttcgacga caatctctat
      241 ggcgaggccg gtaacgacac tctctatggc gccggcgacg tcgacacgat cgacggcggc
      301 gatggcgacg acatcatcta cggcgatttc agtctgatct cctcggccgg cgggatctcc
      361 ggagggcccc ggttcgagac gttgcggggc ggcgccggcg acgacttcgt ctctggcgac
      421 gagggcgacg accggcttta cggcgatgac ggcaacgacc ggctctatgg cggcaccggc
      481 aacgattccc tgaccggcgg cgccggcgcc gaccggatgg agggcggcga gggtaacgac
      541 accggctatg gcggcgacgg cgaagacgtc atcctcgccg gcaccggcac cgaccagctc
      601 agcggggagg gcgataatga tcgcctctat ttcggggccg acttcggcgg atcggacctt
      661 gccgccggcg gagccggatt agaccagctc gtcctccagg ggaattatgc ggcactgact
      721 ctggctgccg ccaatatcgt ctcggtcgag accatccggc tgttgtcggc gggcgattcc
      781 agtatcgtaa ccgctccggg cggcagcttc tcctatgagc tgacggccgc cgattcgctc
      841 gtgtccggca gcaatgtgct gcgcgtcgag gcccccggcc tggcagctgg ggaaaatctg
      901 agattcgacg gttccgccga gaccagcgcg agtttcgcgg tcgtgggggg cgcgggcgcc
      961 gacctgatta ggacgggtgc gggcaacgac tcccttgagg gtggcggcgg agacgacacc
     1021 ctcctcggcg gtgcgggcga cgacctgctg accggcggcg acggcaacga ctcgctgaac
     1081 ggcgaggccg ggaacgacac tctcaccggc ggcgcggggg ccgacaatct catcggcgga
     1141 gatggcaacg acgtctatat cctcgactcc atcgcgcatg cggtgatcga gcacccgaac
     1201 gagggcgtcg acgaggttcg catcggcgtc ccgggctacg tcctccccgc caatgtcgag
     1261 aacctgacct ataccggcgg tttcgaaacc aacctcgtcg ccaacgcggg caacaatgtc
     1321 gtcaccggcg gcgacgcgcg cgaagtctat tacatgcagc agggcggcga cgacatcgtg
     1381 cgcagcttcg gcggcaacga cacgttcgtg ttcggggcga ccttcaacgc cgccgactcg
     1441 atcgaagccg gggccggcac cgacgggctg atcctgcagg gaaattatcc ggccctgacc
     1501 ttcggcacgg gcacgaccag caacatctcg ggcgtcgaga ccatctcgct cgcgcccggg
     1561 cacttcaccg actggggcga cacgtccgga agtttctacg actacaacgt cactacgctg
     1621 aacggaaacg tggccgccgg ggcggtgctg aagttcaacg gcttctacct gcgggcgggc
     1681 gaggatttca ccttcaacgg tgccgccgag accgacggca agttca
//
LOCUS       DASWNU010000384         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_482785, whole genome shotgun sequence.
ACCESSION   DASWNU010000384 DASWNU010000000
VERSION     DASWNU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482785"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..661)
                     /locus_tag="VGB57_07705"
     CDS             complement(74..661)
                     /locus_tag="VGB57_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0131274.1"
                     /translation="MAKGRASKAVLAADEAAAEAALPYQGSRAMEALGRFASLGDQPP
                     LRTLCAAVIAAGVAGGDRRLARAGVRMLVAHTLATLAKDFVKERVDRTRPRSRGEKGK
                     DHVPRPGRDSSKEETSFPSGHSAGAAAVAWAFARDYPEYADPAYAAGSALALAQIPRC
                     AHYPTDVGAGLAIGIAAEAVVDIGFGLVKEPEGPV"
     gene            complement(654..1352)
                     /locus_tag="VGB57_07710"
     CDS             complement(654..1352)
                     /locus_tag="VGB57_07710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0131275.1"
                     /translation="MNEQSMSCGGARPKAVLFDVDGTLVDTNDLHAVAWREAFLNFGL
                     DKPVEEIRWQIGKGGDNLIPSLFPDLGDRQREEIEAFRGDLFKRDYLPRATPFPGVRD
                     LFERLTAEGMKIVLASSSHSEEVDYHLSLIACADLVSATTSKDDVEKSKPCPDIFAAA
                     LGRVAPLGADEVAVVGDTPWDAKAAGKLGLRIVGFRSGGFPDEALAEAGACELYDGPG
                     DLLARFDHSVFGRG"
     gene            1532..1726
                     /locus_tag="VGB57_07715"
     CDS             1532..1726
                     /locus_tag="VGB57_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131276.1"
                     /translation="MQQALKGIFFIMPFLFGIGFIAPVTAELMTVAGIEAPLNFSRIG
                     FGLLFGGAWGLYATVKGSWL"
     gene            1723..>2184
                     /locus_tag="VGB57_07720"
     CDS             1723..>2184
                     /locus_tag="VGB57_07720"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0131277.1"
                     /translation="MRTQAAAPALDRSLSPTVDAPARQPAPASDDPEERARLLKQERD
                     VVARYIAGPDWTRYIYPAIALGGFALWVSLFPLAMAGIVPLWLGCLVSTVIATGGYVT
                     SHEAMHSNIARKGEKLRWLNELTGQVSTIPILFPFSMARIMHLQHHYHCNDP"
BASE COUNT          347 a          751 c          746 g          340 t
ORIGIN      
        1 gcggcgggcg agcaggcggc gaggaaggcg agaaaagcga ggggcaacag gcgcaaggcg
       61 caactcctaa tcttcagacg gggccttccg gctccttcac caggccgaag ccgatgtcca
      121 ctaccgcctc ggccgcgatg ccgatggcga gcccggcgcc gacgtcggtc ggataatggg
      181 cgcagcgcgg gatctgggcc agtgcgagcg cgctgccggc ggcataagcc gggtctgcat
      241 attccggata gtcccgagcg aaggcccagg cgacggccgc ggcccccgcg ctgtggccgg
      301 aggggaagct ggtctcctcc ttcgagctgt cgcggccggg cctgggcacg tgatccttgc
      361 ccttctcgcc cctggatcgc ggccgggtgc ggtcgacacg ctccttgacg aagtccttgg
      421 ccagggtggc gagggtatgc gcgaccagca tccgaacgcc ggcgcgggcg aggcggcggt
      481 cgccgccggc gactcccgcc gcgatcaccg ccgcgcaaag ggtgcgaagc ggcggctggt
      541 cgccgagcga ggcgaagcgg ccgagcgcct ccatcgcccg cgacccctga taaggcaaag
      601 ccgcctctgc ggcggcctcg tcggccgcaa gcacggcctt gctcgcccgc cccttagcca
      661 cggccgaaca ccgaatggtc gaagcgggcg agcaggtcgc cggggccgtc gtaaagctcg
      721 caggcgccgg cctcggccag ggcttcgtcg gggaagccgc cggagcggaa gccgacgatc
      781 ctgagaccga gcttcccggc cgccttggcg tcccagggcg tgtcgccgac cacggccacc
      841 tcgtccgcgc cgagcggcgc cacccggccg agcgccgccg cgaagatgtc cgggcagggc
      901 ttcgacttct cgacgtcgtc cttcgaggtc gtcgcgctca ccagatcggc gcaggcgatg
      961 aggctgagat gatagtcgac ctcctcggaa tgggaggagg aagcgaggac gatcttcatg
     1021 ccctccgcgg tcagccgctc gaacaggtcg cgcactcccg ggaagggcgt ggcgcgcggc
     1081 agatagtccc gcttgaagag gtcgccgcga aacgcctcta tctcctcgcg ctgccggtcg
     1141 cccaaatcgg ggaagagcga agggatgagg ttgtcgccgc ccttcccgat ctgccagcgg
     1201 atctcctcga ccggcttgtc gagaccgaag ttcaggaacg cttcgcgcca ggcgacggcg
     1261 tggagatcgt tggtgtcgac gagggtgccg tcgacgtcga acaggacggc cttcggacgg
     1321 gccccgccgc agctcatgga ttgctcattc atgtccgccc aacggccgag cggatcgcaa
     1381 tatccttcag ggttcgccca aaccctggcc caaatgcgga caggccgtcc cgccggccga
     1441 gatcgtggtt aacgccgcgt tgaccgtcat tgtcgccccg ccccgacttt gctatgtccg
     1501 ggcccagatc aaaaggggcg atccaggggt catgcagcaa gcgctgaagg gtattttctt
     1561 catcatgccg ttcctgttcg ggatcggctt catcgcgccg gtgacggccg agctgatgac
     1621 cgtggccggg atcgaggctc cgctgaactt ctcgcgcatc ggcttcgggc tgctcttcgg
     1681 gggggcttgg ggcctttacg cgactgtgaa ggggagctgg ctgtgaggac acaagccgcg
     1741 gccccggcgc tggaccggag cctttcgccg acggtcgacg cgcccgctcg ccagcccgct
     1801 cccgcgagcg acgatccgga agagcgcgcc cggctgctca agcaggaaag agacgtcgtc
     1861 gcccgctata tcgccggtcc cgactggacc cgatatatct accccgccat cgcgctcggc
     1921 ggcttcgccc tctgggtgtc cttgttcccg ctggcgatgg ccggcatcgt gcccctttgg
     1981 ctcggctgcc tggtctcgac cgtgattgcg accggaggct acgtcacctc ccacgaggcg
     2041 atgcacagca acatcgcccg caagggcgag aagctgcgct ggctcaacga gctgaccgga
     2101 caggtcagca ccatcccgat cctgttcccg ttcagcatgg ctcggatcat gcacctgcag
     2161 catcattatc actgcaacga tccg
//
LOCUS       DASWNU010000385         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_377515, whole genome shotgun sequence.
ACCESSION   DASWNU010000385 DASWNU010000000
VERSION     DASWNU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377515"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VGB57_07725"
     CDS             complement(<1..178)
                     /locus_tag="VGB57_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEY0131278.1"
                     /translation="MRNIGDIMRLAPVIPVLVIEDAADAGTIARALVAGGLRVLEVTL
                     RTPAALDAIAEMRKV"
     gene            complement(175..1146)
                     /gene="glk"
                     /locus_tag="VGB57_07730"
     CDS             complement(175..1146)
                     /gene="glk"
                     /locus_tag="VGB57_07730"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY0131279.1"
                     /translation="MTEIVAIDIGGTHARFALAQIEGGRVLRLGDVCTLETARHAGLQ
                     AAWEAFGAAVGPPLPNAAAIALAGSIQGEVVKLTNNPWAFRLDSLPSAVGIERYIMIN
                     DFGAVAHAVAQLGDEHFSHLCGPERPLPEEGVISIVGPGTGLGVAQLLRREGRYHVIE
                     TEGGHVDFAPLDGLEDRILAQLRLRHRRVSVERVASGMGLANLYEALAAIEGRDVRFR
                     DEKALWQAAMAGGDSLAAAAFDRFCLSLGAVAGDIALTQGAGAVVIGGGLGLRLAGHL
                     PRSGFRGRFIAKGRFGRRMEEMPVKIITYGQPGLFGAAAAFAAEHVE"
     gene            1244..2008
                     /locus_tag="VGB57_07735"
     CDS             1244..2008
                     /locus_tag="VGB57_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY0131280.1"
                     /translation="MADMKRDWTRLVTRAAVVLGVGAVLAALVAAVGSAAGAWGFGTG
                     LKALRYLFFAAAAGALLGLIGLVMARRRAKLMLANLLALVIALGFVLYLGNLVRIAKS
                     VPAIHDVATDLDDVPQFTRLKVRPDNLENIPDEGRPELKALPPEERWKALHRAHYGDV
                     RTLRLPAPPDQVAQRAAALARERGWEVAVAEGDRVEATATSTFFRFKDDVVVRVRPAP
                     GGGSLVDMRSISRVGGSDVGMNARRVRDFLADLQRG"
     gene            complement(2051..>2357)
                     /locus_tag="VGB57_07740"
     CDS             complement(2051..>2357)
                     /locus_tag="VGB57_07740"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEY0131281.1"
                     /translation="PQVENVVVDERGFPLRISAPDPRAFALHKLWLSERLDRDPLKRR
                     RDARQAAAVSAAVLRYLPQLRFDDEALNALPQALRARTSELLGKAAEFLSKLDPDPW"
BASE COUNT          367 a          792 c          862 g          336 t
ORIGIN      
        1 cgaccttgcg catctcggcg atggcgtcga gggcggcggg ggttcgaagg gtgacctcga
       61 gcaccctcag gccgcccgcg acgagggccc gggcgatcgt gccggcgtcg gcggcgtcct
      121 cgatcacgag caccgggatg accggcgcaa ggcgcatgat gtcgccgata ttcctcattc
      181 cacatgctcc gcggcgaaag cggcggccgc gccgaaaagg cccggctgcc cataggtgat
      241 gatcttgacc ggcatctcct ccatccgccg cccgaagcgg cccttggcga tgaagcggcc
      301 ccggaagccc gagcggggca ggtgcccggc caggcgaagg ccgaggccgc cgccgatgac
      361 gacggcccct gcgccctggg tgagcgcgat gtcgccggcg acggcgccga gcgacaggca
      421 gaagcggtcg aaagcggcgg cggcgaggct gtccccgccg gccatcgccg cctgccacag
      481 cgccttctcg tcgcggaagc ggacgtcgcg gccctcgatc gcggcgagcg cctcgtagag
      541 gttggcgagg cccatgcccg aagcgacccg ctcgaccgag acccggcgat ggcggagtcg
      601 aagctgggcg aggatgcggt cctcgaggcc gtcgagaggc gcgaaatcga catggccccc
      661 ctcggtctcg atgacgtgat agcggccttc gcggcggagc agctgggcga ccccgagacc
      721 ggtgcccggg ccgacgatgc tgatcacccc ctcctcgggc aaaggccgct ccggcccgca
      781 caggtggctg aaatgctcgt cgccgagctg ggcgacggcg tgggcgaccg cgccgaaatc
      841 gttgatcatg atgtagcgct cgatacccac cgcggagggg agggagtcga gccggaaggc
      901 ccaggggttg ttggtgagct tcaccacctc gccctggatc gagccggcga gggcgatcgc
      961 cgcggcattc gggaggggtg gtcccaccgc cgcgccgaaa gcctcccagg cagcctgaag
     1021 gccggcatgc cgcgccgtct cgagcgtgca gacgtcgccg aggcgcagaa cgcggccgcc
     1081 ttcgatctgg gccagagcga agcgggcgtg ggtgcctcca atgtcgatgg cgacgatctc
     1141 ggtcatgggc gcgcggtacc aagcctatat cgacccgcac tgcaaggctc ggcccttgca
     1201 gtgatcgccc tctcggcgca gcatggcggc aagggagggc gatatggcgg atatgaagag
     1261 ggactggacc cggctcgtca cgagggccgc ggtcgtgctc ggtgtcggcg cggtgctcgc
     1321 cgctttggtt gcggccgtcg gaagcgcggc gggggcatgg ggcttcggca ccgggctgaa
     1381 ggccttgcgc tacctcttct tcgcggccgc ggccggcgcg ctcctcgggc tgatcggcct
     1441 cgtcatggcg cggcggcggg cgaagctgat gctcgccaac cttctcgctc tggtgatcgc
     1501 tctcggcttc gtcctctatc tcggcaatct ggtgcggatc gcgaagagcg tcccggccat
     1561 ccacgacgtt gccaccgatc tcgacgacgt tccgcaattc acccgcctca aggtgcgccc
     1621 ggacaatctc gagaatatcc cggacgaggg cagaccggag ctcaaggcgc tgccgcccga
     1681 ggagcggtgg aaggccctcc accgggccca ttatggcgac gtccggacgc ttcgcctccc
     1741 ggcgcctccg gaccaggtgg cgcagcgcgc ggccgcgctt gcgcgggagc gcgggtggga
     1801 agtggctgtg gcggagggcg accgggtcga ggcgaccgcg acgagcacct tcttccgctt
     1861 caaggacgac gtcgtcgtcc gggtccgacc ggcgcccggc ggcggcagcc tggtcgacat
     1921 gcgctcgatc agccgggtcg gcggaagcga cgtgggaatg aacgccagga gggtacgcga
     1981 cttcctggcc gaccttcagc ggggctgagc gcaggatcgg ccgaccggcg gcatcggtag
     2041 cgtcagcgcc ttaccaaggg tccgggtcca gtttggacag gaactcggca gccttgccca
     2101 gaagctcgga agtccgtgca cgaagtgcct ggggaagggc attgagcgcc tcgtcgtcga
     2161 agcgcaattg cggcaagtaa cgcagcacgg cggccgagac agcggcggcc tgccgcgcgt
     2221 cgcgccgacg tttcaacgga tcccggtcca gccgttccga aagccagagc ttgtgtagcg
     2281 cgaaagccct tgggtccggc gccgatattc gaagtggaaa tccgcgctcg tccaccacca
     2341 cattctcgac ctgcggg
//
LOCUS       DASWNU010000386         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_274141, whole genome shotgun sequence.
ACCESSION   DASWNU010000386 DASWNU010000000
VERSION     DASWNU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274141"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGB57_07745"
     CDS             <1..346
                     /locus_tag="VGB57_07745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0131282.1"
                     /translation="DVRSWLAAADVVAAPLRIARGIQNKVLEAMAMARPVVASPAAFE
                     GIEAEPGRHLLVAAEAEAQAEAISGLLDDPERARAMGQGARRRMEQVYRWEARLAALG
                     GIVHIPPHKVAA"
     gene            343..1878
                     /gene="xrtA"
                     /locus_tag="VGB57_07750"
     CDS             343..1878
                     /gene="xrtA"
                     /locus_tag="VGB57_07750"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEY0131283.1"
                     /translation="MTAVLPIVRADPLACAWRSHLVALGAAAAAIGLLFARDAVDMAE
                     IWLTSTTFNHCALVLPIVAWLVWQRLPDLKALAPRAWAPGLALVGAGGAAWLLGEAGN
                     VALLRHLGLVLMLQGSTIACLGKAVARGIAFPLFYAFFLVPFGDQLVPPLQTLTAKMS
                     MALLALTGVPAHIEGIFISTPSGLFRVAEACSGVRFLVAMAALAALAANLCFRSWPRR
                     LAFVAFALAVSVLANGVRAFGTIYIAYKTDNEVAVGIDHLIYGWVFFAVVIALIVAVA
                     WRFFDRSPGARWFDPADLQAPGTEPGRPAGAAQMAAIAVALAAVAPLWSSAVAAAGTR
                     AVPMDPLPQVAGWTRLPAGPEWRPNYSGADRIRMARYRNASGQQVDLAIVVFARQSEG
                     RELVGYGQGAADEDGAWSWTGDSAPPAEGKAELIGSHGKVREVASFYRVGDIVTGSAT
                     RVKLETVRVRLLGGPQRAVAVLVAAEAPADGLSPRPAIDAFLHDLGPVAPLADRAAGL
                     AGD"
     gene            1882..>2623
                     /locus_tag="VGB57_07755"
     CDS             1882..>2623
                     /locus_tag="VGB57_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEY0131284.1"
                     /translation="MCGIAGLFYVSNPKPVDPKRVRTMTDTLAHRGPDGSGVWTAPGV
                     GLGHRRLSIIDLATGDQPMRSADGRLALSYNGEIYNFREVRGELEGFGHGFRTESDTE
                     VILAAWRQWGPDCLSRFNGMFAFALYDQDQDCLFLARDRLGVKPLYWTTLSDDALVFA
                     SELKGLLAHPLLRRSPSHRAIEDYLALGYVPDDSCMVAGVRKLAAGHYLLVRRGRVVP
                     KPVQWWDVDFSNPSRASAGALEEELVERL"
BASE COUNT          327 a          954 c          922 g          420 t
ORIGIN      
        1 cgacgtcagg tcctggctcg ccgccgcgga cgtcgtcgcc gccccgcttc gaatcgctcg
       61 gggaatccag aacaaggtgc tcgaggcgat ggccatggcc cgtcccgtgg tcgcctcgcc
      121 ggccgcgttc gaggggatcg aggccgagcc cggccgccac ctcctcgtcg ccgccgaggc
      181 cgaggcccag gccgaggcga tttccggcct cctcgacgat cctgagcggg cgcgggccat
      241 ggggcagggc gcccgccgcc ggatggagca ggtctaccgc tgggaagcgc ggctcgccgc
      301 gctcggcggc atcgtccaca ttccccccca caaggtggcg gcatgacggc ggtccttccg
      361 atcgtccggg cggatccgct cgcttgcgcc tggcgaagcc atctcgtcgc gctcggagcg
      421 gcggcggcgg ccatcgggct cctgttcgct cgcgatgccg tcgacatggc cgagatctgg
      481 ttgaccagca ccaccttcaa ccattgcgcg ctcgtccttc cgatcgtcgc ctggctggtc
      541 tggcagcgcc tgcccgacct caaggcgctg gcgccgaggg cctgggcccc ggggctcgca
      601 ctggtcggag cgggcggcgc ggcctggctt ctcggcgagg cgggcaatgt cgccctgctt
      661 cgccatctcg gcctcgtcct gatgcttcag ggcagcacga tcgcctgcct cggcaaggcg
      721 gtggcgaggg gaatcgcctt tccgctcttc tacgctttct tcctggtgcc gttcggcgac
      781 cagctggtgc cgccgctcca gaccctgacg gcgaagatga gcatggccct gctcgccctg
      841 accggggtcc ccgcgcatat cgagggcatc ttcatctcca cgccgagcgg gctgttccgg
      901 gtcgccgagg cctgctccgg cgtgaggttc ctggtcgcga tggcggctct ggccgcgctt
      961 gccgccaacc tctgcttccg ctcctggccg cggcgcctcg ccttcgtcgc cttcgcgctc
     1021 gcggtctccg tgctcgccaa cggcgtgagg gcgttcggga ccatctacat cgcgtacaag
     1081 accgacaacg aggtcgcggt cgggatcgac cacctcattt acggatgggt cttcttcgcc
     1141 gtcgtcatcg ccctcatcgt cgcggtggcc tggcgcttct tcgaccggtc gcccggcgcg
     1201 cgctggttcg acccggccga cctccaggcg cccggcaccg agccgggccg cccggccggc
     1261 gcggcgcaga tggccgcgat cgcggtcgcc ctcgcggcgg tggcgccgct ctggtccagc
     1321 gcggtcgccg ccgccgggac ccgggcggtg ccgatggacc cgcttcccca ggtcgccggc
     1381 tggacccggc ttcccgcggg gccggaatgg cggccgaatt attcgggcgc cgaccggatc
     1441 cggatggccc gttaccgcaa cgcctccggg cagcaggtcg atctcgcgat cgtcgtcttc
     1501 gcccggcaga gcgaagggcg cgagctggtc ggctacggcc agggcgccgc cgacgaggac
     1561 ggcgcctggt cgtggaccgg cgattcggcg ccgccggccg agggcaaggc ggaactgatc
     1621 ggatctcacg gcaaggtgcg cgaggtggcg agcttctacc gggtcggcga tatcgtcacg
     1681 ggaagcgcca caagggtgaa gctggagacg gtcagggtgc gcctgctcgg agggccgcag
     1741 cgggcggtgg cggtgctggt cgcggcagag gctccggccg acgggttgag cccgaggccg
     1801 gcgatcgacg ccttcctgca cgacctcggg ccggtcgcgc cccttgccga ccgggccgcc
     1861 gggctggcag gggactgagc catgtgcggc atcgccggtc ttttctacgt gagcaacccc
     1921 aagcccgtgg acccgaagag ggtgcggacg atgacggaca cgctcgccca tcgcgggccg
     1981 gacggaagcg gcgtgtggac cgctcccgga gtcggcctcg gccaccggcg gctgtcgatc
     2041 atcgacctag ccaccggcga ccagccaatg cgctccgccg acggcaggct tgcgctcagc
     2101 tacaatggcg aaatctacaa tttccgcgag gtgagggggg aactcgaggg cttcggccac
     2161 ggcttccgga ccgagagcga caccgaggtg atcctggccg cctggcggca atggggcccc
     2221 gactgcctct cccgcttcaa cggcatgttc gccttcgccc tctacgacca ggaccaggat
     2281 tgcctgttcc tcgcccgcga ccggctcgga gtgaagccgc tctactggac gacgctctcc
     2341 gacgatgccc tggtcttcgc ctccgagttg aagggccttc tcgcccatcc cctgctccgc
     2401 cgctcgccca gccaccgggc gatcgaggat tatctcgcct tgggctacgt cccggacgac
     2461 agctgcatgg tcgccggggt gaggaagctc gcggccggcc attacctgct cgtccgccgc
     2521 ggccgggtgg tgcccaagcc ggtccaatgg tgggacgtgg acttctccaa cccctcccgc
     2581 gcctccgccg gggcgctcga ggaggaactg gtcgagcggc ttc
//
LOCUS       DASWNU010000387         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_430410, whole genome shotgun sequence.
ACCESSION   DASWNU010000387 DASWNU010000000
VERSION     DASWNU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430410"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGB57_07760"
     CDS             <1..341
                     /locus_tag="VGB57_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0131285.1"
                     /translation="TSNTPALAQRVRSAWSRALPAGTVVPFAQESMGAEDFTYFVQPE
                     LGVAGFYFSVGGTPGAAFDAARAGGPPVPSHHSPLFRIDPERSIRLGTEAMTVAVLDL
                     LRPGGSAAGN"
     gene            364..933
                     /locus_tag="VGB57_07765"
     CDS             364..933
                     /locus_tag="VGB57_07765"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HEY0131286.1"
                     /translation="MKTRLILGAAALALAACGSSGGEKGGEGGNGSAAAAASGGSGGS
                     GGSASGVNLQPGEWEMKMEVVNVKVEGLPPGLAENMKSQSGGINRTCMTPEEAKAPKG
                     DIFAGDKSNNCKSEGFKWEGGRIEGKTTCSGQGGTGQTVMTMDGKYSAQSIDMTMKSE
                     TDMMGKPMTMEMRVSGKRVGECTAATKEG"
     gene            complement(1103..2203)
                     /locus_tag="VGB57_07770"
     CDS             complement(1103..2203)
                     /locus_tag="VGB57_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0131287.1"
                     /translation="MRTWLRRLGTLILFLAVGLCFAPSLIPPFLDRVYYEGPRSAHFD
                     GERFFNPTPGPASTAHGGPARFMNRFIGSNERAQWPEHVPVAPTVPPRRVEGSEMLVT
                     WIGHATVLVQTRGLNILADPIWSERASPFSFAGPKRVRAPGVRFEDLPKIDLVLVSHN
                     HYDHMDLPTLKRLWQRDRPAIVTSLGNDTILKGEGINAIALDWGGGWSVAPLCPPEAP
                     CGEIDVRVERNHHWGSRWGTDRNRALWSAFTVRLPGGNVFFAGDTGYGDGSWADEAAR
                     RGRIRLAVLPVGAYEPRDVMKANHIDPDEAVRIFEKLKPRTALGMHWGTFQLTFESID
                     DPPGRLAKALKARGIAPERFVTTEVGRSFRVP"
BASE COUNT          396 a          716 c          868 g          324 t
ORIGIN      
        1 cgaccagcaa cacgccggcg ctggcccagc gggtccgctc cgcctggagc cgggcgcttc
       61 cggcggggac ggtggtcccg ttcgctcagg agagcatggg cgccgaggac ttcacctatt
      121 tcgtccagcc cgagctcggc gtggccggct tctacttctc ggtcggcggc actccggggg
      181 cggcgttcga tgccgcccgg gcgggagggc cgccggtgcc gtcccatcat tcgcctttgt
      241 tcaggatcga cccggaacgg tcgatccggc tgggtaccga agcgatgacg gtcgcggtgc
      301 tcgacctgtt gaggccaggc gggagcgcgg ccgggaattg atccacaaga gggggtaaag
      361 ccaatgaaga ctcgactgat cctcggcgcc gcggcgctgg cgctcgcggc ctgcggaagc
      421 tccggcggag aaaaaggggg cgaaggcggc aacggctcgg ccgccgcggc ggcaagcggc
      481 gggagcggcg ggagcggcgg ctccgcttcg ggcgtgaacc ttcagcccgg cgaatgggaa
      541 atgaagatgg aggtggtcaa cgtgaaggtg gagggacttc cgccgggcct cgccgagaac
      601 atgaagagcc agtccggcgg catcaaccgc acctgcatga ccccggaaga ggccaaggcc
      661 ccgaagggcg atatcttcgc cggcgacaaa tcgaacaatt gcaagtccga aggcttcaaa
      721 tgggagggcg gccggatcga gggaaagacg acctgctccg gccagggcgg caccggccag
      781 acggtgatga ccatggacgg caagtatagc gcgcagagca tcgacatgac gatgaaatcg
      841 gaaaccgaca tgatgggcaa gccgatgacg atggagatgc gggtctccgg caaacgagtc
      901 ggcgagtgca cggcggccac caaggaaggc tgagcgacga tcggcttgcc cgtgcggctg
      961 gggcgcgcgt aaccaaatcc tccccgcgat ttcgcgggga gggggaccgc gcgaagcgcg
     1021 gtggaggggt tcttcggagt cggcaaggcc cctccaccag ccttcggctg gcccccctcc
     1081 cctgcaacgg caggggagga atttagggca ccctgaagct tcgcccgact tcggtcgtca
     1141 cgaacctttc cggcgcgatg ccgcgcgcct tgagcgcctt cgccaggcgc ccgggcggat
     1201 cgtcgatgct ctcgaaagtg agctggaagg tgccccagtg cattccaagc gccgtccgcg
     1261 gcttcagctt ctcgaatatc cgaaccgcct cgtccgggtc gatatgattg gccttcatga
     1321 cgtcgcgcgg ctcgtaggcg ccgacgggca ggacggcgag gcggatgcgg ccgcggcgcg
     1381 cggcctcgtc ggcccagctg ccgtcgccat agccggtgtc gccggcgaag aagacattgc
     1441 cgccaggaag cctgacggtg aaggcggacc agagggcgcg gttgcggtcg gtgccccagc
     1501 gcgagcccca atggtggttg cgctccaccc gaacgtcgat ctcgccgcac ggggcctcgg
     1561 gcgggcagag cggcgcgacc gaccagccgc cgccccagtc gagggcgatc gcgtttatgc
     1621 cttcgccttt gaggatcgtg tcgttgccca ggctggtgac gatggcgggg cggtcccgct
     1681 gccaaagccg cttcagcgtc ggcaggtcca tatggtcgta atggttgtgg ctgacgagga
     1741 cgaggtcgat cttcggcagg tcttcgaacc gcacccccgg ggcccggacg cgcttcggac
     1801 ccgcgaagga gaaaggcgag gcgcgctccg accagatcgg gtcggcgagg atgttgaggc
     1861 cgcgggtctg gaccaggacg gtggcgtggc cgatccaggt gacgagcatc tccgagccct
     1921 cgacccggcg cggcggaacg gtcggcgcga ccggtacatg ctccggccat tgcgcacgct
     1981 cgttcgagcc gatgaagcgg ttcatgaagc gggccgggcc gccatgggcc gtggaggcgg
     2041 ggcccggggt ggggttgaag aagcgctcgc cgtcgaaatg ggcggagcgg gggccttcat
     2101 aatagactcg gtcgaggaag ggcgggatca gcgacggcgc gaagcacagg ccgacggcga
     2161 ggaagaggat gagggtgccg aggcggcgga gccaggttcg catcgccgct tcctagccgc
     2221 cgccgaggcc ggggccaatc agcgcgcgtc gagggcgaag ccgggggccg gccgcgtctc
     2281 gacatgctcc tcatggtaga aatc
//
LOCUS       DASWNU010000388         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_119638, whole genome shotgun sequence.
ACCESSION   DASWNU010000388 DASWNU010000000
VERSION     DASWNU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119638"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2140
                     /locus_tag="VGB57_07775"
     CDS             <1..>2140
                     /locus_tag="VGB57_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0131288.1"
                     /translation="DHVSGGGSEDFVDYSMRYAGETGTIGVIVNLSAQAYSIHAGHAL
                     AGGSVAPNEAIDNWGGRDTLVGIESAVGGDLDDVMIGSDFQNNSFSGNGGNDRFVGGS
                     GSDRFFGGSGNDLVDYSAESGTGGIFVNLEGSGTLVLAPGGTSVSPGRAIDSFGAPDT
                     LAGIEQITTGGQADTVQGNGLDNRISLGAGSDVGYGGGGDDLIIGAEGDDRLYGGSGG
                     DRLEGGDGSDTLVGDPGADSIDAGAGDDSVQAGADNDIVRGGGDNDDLGGDDGDDQLF
                     GDSGNDNMRGGRGVDSFDGGSGGEALDLNSTLYGDKVTFFDQFATQSAVADLRTGIIS
                     NDGFGNGETMVNIESLGAGTAFADTFYGNDNRNGLLGSIGDFLHGFGGDDHIDLEGAV
                     ALIDGGDGVDSIRLRIHGALLLPNEAGFADYMAAMTSGWKVDLAAGTLRDGYGNSGNV
                     AGIENVTGSELADDLRGSALDNRVDGGGGADVLRLHDGGDDTVNGGTGNDNIFFIGSL
                     TAADVVNGGEGTDTLILQGAYGSLTLTANVTQIENISILGGNNTAFGGSSGNRHDYVL
                     TTHDSNFAAGIQARINGAALLAGEDFTFDGSAETDALFVVYGGKGRDTLTGGQGNDIF
                     FYAEERFASGDTVNGGAGYDGMFLRGNYTIDFNAPGYTGLFTNIENLTLSSATDERFA
                     RGGGTEFDYNLTLSDAIVNPGYQLTVSGAVL"
BASE COUNT          382 a          692 c          747 g          319 t
ORIGIN      
        1 gaccatgtca gcggcggcgg aagcgaggac ttcgtcgact attcgatgcg ctatgccggc
       61 gaaaccggga ccatcggcgt gatcgtcaac ctctcggccc aggcttattc gatccacgcc
      121 ggtcacgcgc tcgccggcgg ctcggtggcg cccaacgagg ccatcgacaa ttggggcggc
      181 cgcgatacgc tggtcgggat cgagagcgcc gtgggcggcg acctggacga cgtcatgatc
      241 ggcagcgact tccagaacaa cagtttctcg ggcaacggcg gcaacgaccg cttcgtcggg
      301 ggctcgggct ccgatagatt tttcggcggc agcggaaatg acctggtcga ttattcggcg
      361 gagtcgggga ccggcgggat cttcgtcaac ctggaaggaa gcggcaccct cgtcctcgcg
      421 ccgggcggga cgtccgtgtc gccggggcgg gcgatcgaca gcttcggcgc ccccgacacc
      481 ctcgcgggga tcgagcagat caccacgggc ggccaggccg atacggtcca gggcaacggc
      541 ctcgacaacc ggatctccct cggcgccggc agcgacgtcg ggtacggcgg gggcggagac
      601 gatctgatca tcggagcgga gggcgacgac cggctttacg gcggctccgg cggcgaccgg
      661 ctcgaagggg gcgacggctc cgacactctg gtcggcgacc cgggcgccga ttcgatcgac
      721 gcgggcgctg gcgacgattc cgtgcaggcg ggggcggata acgacattgt gcgcggcggc
      781 ggcgacaacg acgaccttgg cggcgacgac ggcgacgacc agctgttcgg ggattcgggc
      841 aacgacaaca tgcgcggcgg gcgaggcgtc gacagcttcg acggtggcag cggcggcgag
      901 gcgctggacc ttaactccac cctctacgga gacaaggtca ccttcttcga tcagttcgcg
      961 acccaaagcg cggtggcgga cctgaggacg gggatcatct cgaacgacgg cttcggcaat
     1021 ggcgagacga tggtcaacat cgagtcgctg ggcgccggca ccgccttcgc cgacaccttc
     1081 tacggcaacg acaatcgcaa cggcctgctc ggcagcatcg gcgacttcct ccacggattc
     1141 ggcggcgacg atcatatcga cctggagggc gccgtggcac tgatcgacgg cggcgacggg
     1201 gtcgactcga tcaggctccg gatccacggc gctttgctcc tcccgaacga agcgggcttc
     1261 gccgattaca tggccgccat gacgtcgggc tggaaggtgg acctcgccgc cggcactctg
     1321 cgcgacggct acggcaacag cggtaacgtc gccggaatcg agaatgtcac cggcagcgag
     1381 cttgctgacg accttcgagg aagcgcgctc gacaaccggg tcgacggcgg cggcggggcc
     1441 gacgtcctgc gcctgcacga cggcggcgac gacacggtga acggcggcac cggcaacgac
     1501 aacatcttct tcatcgggtc gctgacggcg gcggatgtgg tgaacggcgg cgagggcacc
     1561 gacacgctca tcctccaggg tgcttacggc tcactgacgc tgacggccaa cgtcacccag
     1621 atcgagaata tctcgatcct cggcggcaac aacacggcgt tcggcgggtc gagcggcaac
     1681 cgccacgatt acgttctgac cacccacgac tcgaacttcg ccgccggcat tcaggcgcgg
     1741 atcaacggcg ctgcgctgct ggcgggcgag gacttcacct tcgacggctc ggcggagacg
     1801 gatgcgctct tcgtcgtcta cggcggcaag ggcagggata cactgaccgg cgggcagggc
     1861 aacgacatct tcttctacgc cgaggagcgg ttcgcgagcg gggacacggt caacggcggc
     1921 gccggctatg acgggatgtt cctgaggggc aactacacga tcgacttcaa cgctccaggg
     1981 tacaccggcc tgttcaccaa catcgagaat ctgacgctct cctcggctac cgacgagcgt
     2041 ttcgcgcgcg gcggcggcac cgagttcgac tacaacctca ccctttccga cgcgatcgtg
     2101 aatccggggt accagctgac ggtgtcgggc gcggtgctga
//
LOCUS       DASWNU010000389         4309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_15593, whole genome shotgun sequence.
ACCESSION   DASWNU010000389 DASWNU010000000
VERSION     DASWNU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15593"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VGB57_07780"
     CDS             <1..1355
                     /locus_tag="VGB57_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0131289.1"
                     /translation="APSSKNRAAAGGLQIESQYYTLPNGLKVVLSRDPSVPTATIGVY
                     YGIGFRIEPRNRTGFAHLFEHLLFQGSEHAPKGTFIGSITNAGGALNGSTRFDYTNYY
                     EVVPSNALETVLWVEGDRMRGLDLTPAALANQQGVVANEVKVNVLNQPYGGWPWIDMP
                     MLANTNWHNSHNFYGELKDLEAATAADARTFFDSFYRPNNAVLVVAGDIDYGRTRAMI
                     DRYFRGIPKGPKVALPDIGEPRQTQEKFRVRPDPLAPRPAFTAAWHVPERGTKEWYAM
                     GLLDQILVQGQDSRLYQKLVRETGIAGGVQGGINIGLGNMYNYRGPMLWTVAFIHDPA
                     KSREEITAALNSVIEDVRTRPVAAAELERARTKIRSNLYNLADPSTRFGLVDLLAVGA
                     MWENDPNWVNRLEAGFNAVTPELLLATAKEYLRPTNRSILLVQPAPPAAAAPATGASK
                     "
     gene            1352..2779
                     /locus_tag="VGB57_07785"
     CDS             1352..2779
                     /locus_tag="VGB57_07785"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0131290.1"
                     /translation="MIRRVLLVLGAAALGLAAAPLAAQPKYEYPPMPAGGTPKPFAVP
                     ASESYRLANGLQVTLIPYGLVPKASVQMRVYAGSLNEGEDVGLAALAAQMLREGAAGR
                     SGADIAAAAAAMGGNLSVGAGAHETFFVLNVLSEHADDAVRLIADVGQRPDLPESEFE
                     RVRQSYARNVAVNKSQPQSAADAALAAAYYGPKHPYGRLFPTDAQLAAFTADDVRRFY
                     RSNFGAKRARLFVAGRFDKAAVKAAIEQAFGGWAAGPERLSLPPTPQPGPRVILVDRP
                     GAPQSTLRLAFPAPVAGAPEDIGFRVTNALLGGSFTSRITANIREQKGYTYSPYSSLS
                     HNPGETQWGFDADVTTDVTGAALKEVFGEIRRLQTEPVPDEEAAGMRTWMAGTFVLQN
                     ASPAGLIQSVSERDFHGLPASWLSDYVPNVLRVTGADMQRLAGRSLPLDKMTLVVVGD
                     LAKVEPQLKALPELQGVPFQRVTPF"
     gene            complement(2913..3479)
                     /gene="pgsA"
                     /locus_tag="VGB57_07790"
     CDS             complement(2913..3479)
                     /gene="pgsA"
                     /locus_tag="VGB57_07790"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY0131291.1"
                     /translation="MLTLPNILTLSRIFAVPLLVYLLWKPSWYDYAITFGLYCLVGIT
                     DYFDGYLARAQGTVSRLGVFLDPIADKIMVVGVIVMLVSTRQPNAETIIHGFHIVPAL
                     IILLREIAVSGLREFLAGIQVSLPVSRLAKWKTTLQLVALGALILGGAVPQFLWVHWV
                     GLASLWAAATLTLVTGWDYLRVGLKHMD"
     gene            3701..>4309
                     /locus_tag="VGB57_07795"
     CDS             3701..>4309
                     /locus_tag="VGB57_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0131292.1"
                     /translation="MQDSMHLLRKRRFLPLFVTQFLGAFNDNLFKTAMVILVTYYVYS
                     DPTKEATFNAVAGAVFILPFFLFSALAGQLADSTDKARIVRFVKTAEIVIMVFGAAGL
                     LLHNIPLMLAALFAMGIHSSFFGPIKYAVLPQHLRKDEVLGGTGLVEAGTYVAILMGT
                     IAGGLIKPPVAAVAVLAVALLGRIAGQQVPPAPPETDAPPLEM"
BASE COUNT          722 a         1544 c         1368 g          675 t
ORIGIN      
        1 cagcaccatc gtccaaaaac cgggccgccg ctggcggact gcagatcgag agccaatatt
       61 atacgctgcc caacggcctc aaggtcgttc tgtcgcgaga tccgtcggtg ccgacggcaa
      121 cgatcggcgt ctattacggc atcggcttcc gcatcgaacc gcgcaaccga accggttttg
      181 cccatttgtt cgagcatctt ctgttccagg gttccgagca tgcgccaaag ggcactttca
      241 tcgggtcgat caccaacgcc ggcggcgcgc tcaacggctc cacccgcttc gactacacca
      301 attattacga ggtggttccg tccaacgcgc tggagaccgt gctctgggtc gagggcgaca
      361 ggatgcgcgg gctcgacctg actccggctg cgctcgccaa ccagcagggc gtcgtcgcca
      421 acgaggtcaa ggtcaacgtc ctcaaccagc cctacggcgg ctggccgtgg atcgacatgc
      481 cgatgctggc caacaccaac tggcacaaca gccacaattt ctacggcgag ctcaaggacc
      541 tggaggccgc caccgccgcc gacgcgagga ccttcttcga cagcttctac cggcccaaca
      601 acgccgtcct cgtcgtcgcc ggcgacatcg attacgggcg cacccgggcg atgatcgacc
      661 gctatttccg gggcatcccg aaagggccga aagtcgccct gccggatatc ggcgagccga
      721 ggcagacgca ggagaagttc agggtcaggc ccgacccgct ggcgccgcgc cccgccttca
      781 ccgccgcctg gcacgtgccg gagcgcggga cgaaggaatg gtatgcgatg gggcttctcg
      841 accagatcct cgtccagggg caggacagcc ggctctacca gaagctggtg cgcgagaccg
      901 ggatcgccgg cggggtccag ggcgggatca acatcggcct cggcaacatg tacaattatc
      961 gaggcccgat gctgtggacg gtcgccttca tccacgatcc ggccaagtcg cgcgaggaga
     1021 tcaccgccgc cttgaacagc gtgatcgagg acgtccgcac ccggcccgtc gccgccgccg
     1081 aactggagcg ggcccggacc aagatccgct ccaacctcta caacctcgcc gatccctcga
     1141 cccgcttcgg gctcgtcgac ctgctcgcgg tcggagcgat gtgggagaac gatcccaatt
     1201 gggtcaaccg gctcgaggcc ggcttcaacg cggtgacccc ggagcttctg ctcgccaccg
     1261 cgaaggaata tctgcggccg accaaccgct ccatcctcct cgtccagccg gcccctccag
     1321 cggccgccgc ccccgccacc ggagcctcga aatgatccgc cgcgtcctcc tcgtcctcgg
     1381 cgccgccgct ctcggcctcg ccgccgcgcc gctcgccgcg cagccgaaat acgaatatcc
     1441 gccgatgccg gcgggcggca cgcccaagcc cttcgccgtc ccggcctcgg agagctaccg
     1501 gctcgccaac ggcctgcagg tcacgctcat cccttacggc ctggtgccca aggcgtcggt
     1561 ccagatgcgg gtctatgcgg gcagcctcaa cgagggcgag gacgtcggcc tcgcggcgct
     1621 cgcggcgcag atgcttcgcg agggcgccgc cgggcgctcc ggagcggaca tcgccgcggc
     1681 ggccgcggcg atgggcggca atctcagcgt cggagccggc gctcacgaga cgttcttcgt
     1741 cttgaacgtc ctgtccgaac atgccgacga cgcggtccgt ctgatcgcgg acgtcggcca
     1801 gcggccggac ctccccgagt ccgagttcga gcgggtcagg cagagctatg cccgcaacgt
     1861 cgccgtcaac aagtcgcagc cccagtcggc cgccgacgcc gccctcgccg cggcttatta
     1921 cggcccgaag catccctacg gccgcctctt cccgaccgac gcccagctcg cggccttcac
     1981 ggccgacgac gtgcgccgct tctaccgctc caatttcggg gcgaaaaggg cgcggctgtt
     2041 cgtcgccggc cgcttcgaca aggctgcggt caaggccgcg atcgagcagg ccttcggcgg
     2101 ctgggccgcg ggacccgagc ggctcagcct gccgccgact ccgcagccgg gccccagggt
     2161 gatcctcgtc gaccgcccgg gggcgccgca atcgacgctt cgcctcgcct tccccgcgcc
     2221 ggtcgccgga gcgcccgagg atatcggctt cagggtcacc aacgcccttc tcggcggctc
     2281 cttcacctcg cgcatcaccg ccaacatccg ggagcagaaa ggctatacct actcgcctta
     2341 ttccagtctg tcgcacaacc ccggcgagac ccagtggggg ttcgacgcgg acgtcaccac
     2401 cgacgtcacc ggagcggcgc tgaaggaagt gttcggcgag atcaggcgcc ttcagaccga
     2461 gccggtgccg gacgaggagg cggccgggat gcggacctgg atggcgggca ccttcgtcct
     2521 ccagaatgcg agcccggcgg gcctcatcca gtcggtgtcc gagcgcgact tccacggcct
     2581 gccggcaagc tggctgagcg actatgtgcc gaacgtgctt cgcgtcaccg gcgccgacat
     2641 gcagcgcctc gccggccgct cgctgccgct ggacaagatg accctcgtcg tcgtcggcga
     2701 cctcgccaag gtcgagccgc aactgaaggc gctgccggag cttcagggcg ttccgttcca
     2761 gcgggtgacg ccgttctgac ctgcctcccc atgcggaacg catggggagg gggaccaccg
     2821 aaggtggtgg aggggtagct ggcacaggcc tgcgccatta cccctccccc gctacgcggg
     2881 tccccctccc catctccgat ggggaggcaa gctcaatcca tatgcttcag gccgaccctt
     2941 agataatccc agccggtcac cagcgtaagc gtcgccgccg cccaaaggct ggcgaggccc
     3001 acccaatgca cccagaggaa ttgcggaacc gccccgccga ggatcagcgc tccgagcgcg
     3061 accagctgaa gcgtcgtctt ccacttggcc agcctgctga cgggcagtga cacctggatc
     3121 cccgcgagga actcgcgaag gcccgaaacc gcgatctcgc gaagcaggat gatcagggcc
     3181 ggaacgatgt gaaagccgtg gatgatcgtc tcggcgttgg gctggcgcgt cgagaccagc
     3241 atgacgatga ctcccacgac catgatcttg tcggcgatcg gatcgaggaa gactccgagc
     3301 ctcgacaccg tcccctgggc tcgggcgaga tagccgtcga aataatcggt gatcccgacg
     3361 aggcagtaga ggccgaaggt gatggcgtaa tcgtaccagc tcggcttcca cagcaggtag
     3421 acgagcagcg gcaccgcgaa tatccgcgac aaggtgagga tgttgggcag tgtcagcatc
     3481 ggctgactgc gtcccttagc gggtcggggc gcggctgtga acggcggacg cgcccgccgt
     3541 catcccggcg agagccggga gctcggggag accgccgaga cttctcgtca cgaggtcccg
     3601 gctttcgccg ggatgacggt agcgtttgac ccacgcgccg tagcggctat gaccgcccgc
     3661 gcatgtcccg acgcacccag accgcccgcg cctgagcgcc gtgcaggatt cgatgcacct
     3721 cttacgcaag cggcgcttcc tgccgctgtt cgtcacccag ttcctggggg cgttcaacga
     3781 caacctcttc aagacggcga tggtgatcct cgtcacctat tacgtctatt ccgacccgac
     3841 caaggaggcg acgttcaacg ccgtggcggg ggcggtcttc atcctccctt tcttcctctt
     3901 ctcggcgctg gccggccagc tcgccgattc gaccgacaag gcgcggatcg tccgattcgt
     3961 gaagacggcc gagatcgtca tcatggtctt cggagcggcc ggcctgctcc tccacaacat
     4021 cccgctgatg ctcgccgccc tgttcgcgat gggcattcat tcctccttct tcgggccgat
     4081 caaatatgcg gtgcttccgc agcacctgag gaaggacgag gtgctgggcg gcaccggcct
     4141 cgtcgaggcg ggcacctatg tcgccatcct gatggggacg atcgccggcg ggctgatcaa
     4201 gcctcccgtc gccgccgtcg ccgttctggc ggtcgccttg ctcgggcgga tcgccggcca
     4261 gcaggtgccg ccggcgccgc cggaaacgga cgctccgccg ctcgagatg
//
LOCUS       DASWNU010000390         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_403743, whole genome shotgun sequence.
ACCESSION   DASWNU010000390 DASWNU010000000
VERSION     DASWNU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403743"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VGB57_07800"
     CDS             <1..1395
                     /locus_tag="VGB57_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0131293.1"
                     /translation="PGDSVANLNASLRGYFYDYREIGTRTGPGLIDRVRRVTNDEDEW
                     IWEVGGDYELALAGGRLKVIGLARSDHDPFEQGLRTEFADGRPDTGSRFLNIADTQER
                     IARAEYRWKWGEGDWQISAEGAFNSLDNVTEFLELQPDGTFTEIELPGGTATVKEDRY
                     EVMASHGRPLSSKLTVQLAAGGEYSNLRQIGGGGLARAFWRPKGSFSAAWKANPRLDV
                     NFKLQRRVGQLNFGDFLATVNLTDDRENAGNVELVPTQSWEADLEATRNLGRYGTTTL
                     RLYGRLYDDIVDTIPIGLTGESRGNIDRASAYGAEWKTTFNFDPLGWKGARLDARFQA
                     QKTRVKDPLTGDYRSISSNLLHLIDLTLRHDIPGSDWAWGGNMNYGRYARDYRLTEVG
                     RLSEGPVWASAYVENKDVMGLTVRATVSNIFAATSMWDRTVYVGRRTGPIQQIERRDR
                     LIGPIFSFQVRGKF"
     gene            complement(1489..2502)
                     /locus_tag="VGB57_07805"
     CDS             complement(1489..2502)
                     /locus_tag="VGB57_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0131294.1"
                     /translation="MHELALPLLIGLIAIALAFDFLNGLHDAANSIATVVATRLLSPV
                     QAVVFAAFFNFAAYFLALAVPSLHKVADTIGKGLIDKDLITPAVVFGALIGAMFWNVV
                     TWLKGIPSSSSHALVGGLVGAGVAHAGLTGVQWTGLNKTLIAIVLSPMLGMMLAMLIM
                     LLTSWLAFRASARGAEGKFRALHLVSSAAYSVGHGLNDAQKTMGIITVLLYSTGYLTG
                     EFHVPHWVAISCYVAIGLGTLSGGWRIIKTMGTGITKLSQHQGFSASAGGSVMLFTAS
                     WLGIPVSTTHTITGSIIGAGVARRASAVRWGVAQNVVTAWVVTIPASATVGAIFYWLT
                     TLF"
BASE COUNT          475 a          830 c          846 g          380 t
ORIGIN      
        1 ccgggcgatt cggtcgccaa cctcaacgct tcgctgcgcg gctatttcta tgattatcgc
       61 gagatcggaa cccggaccgg gccgggcctg atcgaccggg tgaggcgggt cacgaacgac
      121 gaggacgaat ggatctggga agtcggcggc gactatgagc tggcgctcgc cggcggccgg
      181 ctcaaggtga taggcctcgc gcgctccgac cacgatcctt tcgagcaggg cctgcgcacc
      241 gagtttgccg acgggcgtcc ggacaccggc agccgcttcc tcaacatcgc cgacacccag
      301 gagcgcatcg cccgcgccga atatcgctgg aaatggggcg aaggcgactg gcagatctcg
      361 gcggaggggg cgttcaacag cctcgacaac gtgaccgagt tcctcgagct gcagcccgac
      421 ggcaccttca ccgagatcga gcttcccggc ggcaccgcga cggtcaagga ggaccgctat
      481 gaggtgatgg cgagccacgg ccgaccgctc tcgtcgaagc tcaccgtcca gctcgccgcc
      541 ggcggcgaat attcgaacct gcgccagatc ggcggcggcg gcctggcgcg ggccttctgg
      601 agacccaagg gctccttctc cgcggcctgg aaggccaatc cgcggctcga cgtcaacttc
      661 aagctccagc gccgggtagg ccagctcaat ttcggagact tcctcgccac cgtgaacctg
      721 accgacgacc gcgagaatgc cggcaacgtc gagctcgtcc cgacccagag ctgggaggcc
      781 gatctcgagg cgacccgcaa tctggggcgc tacggcacca ccaccctgag gctctacggc
      841 cgcctctacg acgatatcgt cgacaccatc ccgatcggcc ttaccggcga gtcgcgcggc
      901 aatatcgacc gcgccagcgc ctacggcgcc gagtggaaaa cgaccttcaa cttcgatcct
      961 ctgggctgga agggcgccag actcgatgcc cgcttccagg cccagaagac tcgcgtcaag
     1021 gacccgctga ccggcgacta tcggtcgatc agcagcaatc tcctgcattt aatcgacctc
     1081 accttgcgcc acgacatccc gggcagcgac tgggcctggg gcggcaacat gaattacggc
     1141 cgctacgcca gggactatcg cctcaccgag gtcgggcgcc tgtcggaggg accggtctgg
     1201 gccagcgcct atgtcgagaa caaggacgtg atgggcctca ccgtccgggc gaccgtcagc
     1261 aacatcttcg ccgcgaccag catgtgggac cggaccgtct acgtcggtcg ccggaccggc
     1321 cccatccagc agatcgagcg ccgcgaccgg ctgatcggcc cgatcttctc gttccaggtg
     1381 cgagggaagt tctgaccttc agcccgtcat tgcgaggagc gaagcgacga agcaatccag
     1441 cgggcctccg gggctgcgct ggattgcttc gcttcgctcg caatgacgtc agaacaaagt
     1501 cgtgagccag tagaagatcg cccccaccgt ggccgaggcg gggatggtga cgacccaggc
     1561 cgtgaccacg ttctgggcga cgccccagcg taccgccgag gccctgcggg caactccggc
     1621 gccgatgatc gagccggtga tcgtgtgggt ggtggaaacc ggtatgccga gccaggacgc
     1681 cgtgaacagc atcacggatc cgccggccga ggcgctgaag ccctggtgct gcgagagctt
     1741 ggtgattccc gtgcccatcg tcttgatgat ccgccagccg ccgctcaagg taccgaggcc
     1801 gatcgcgaca tagcagctga tcgccaccca atggggcacg tggaactccc cggtgagata
     1861 gccggtcgaa taaaggagga cggtgatgat ccccatcgtc ttctgggcgt cgttgaggcc
     1921 gtggccgacc gagtaagcgg cggaggagac gagatggagg gctcggaatt tgccctcagc
     1981 gccccgcgcg ctcgcgcgga acgccagcca gctcgtcagg agcatgatca gcatcgccag
     2041 catcatgccc agcatcggag acaggacgat cgcgatcagg gtcttgttga gcccggtcca
     2101 ctggaccccg gtcaggccgg catgggcgac tccggcgccg actaggccgc cgaccagcgc
     2161 atggctggac gaggagggga tgcccttcag ccaggtgacg acgttccaga acatcgcccc
     2221 gatcagagcc ccgaagacca cggcgggcgt gatcagatcc ttgtcgataa ggcccttgcc
     2281 gatcgtgtcc gcgaccttgt gaaggctcgg cacggcgagg gcgaggaagt aggcggcgaa
     2341 gttgaagaag gcggcgaaga cgaccgcctg caccgggctg agcagccggg tcgcgacgac
     2401 ggtcgcgatc gagttggccg cgtcgtggag cccgttcagg aaatcgaatg ccagggcgat
     2461 ggcgatgagg ccgatgagga gcggaagggc cagctcgtgc atggtcccgg tccctcaggc
     2521 gtggtcgatg a
//
LOCUS       DASWNU010000391         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_40981, whole genome shotgun sequence.
ACCESSION   DASWNU010000391 DASWNU010000000
VERSION     DASWNU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40981"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VGB57_07810"
     CDS             <1..1219
                     /locus_tag="VGB57_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131295.1"
                     /translation="LSPGSLLDASLGRRVHLRRTSRATGKVRETEAVVRSGAAGAVVL
                     ETPDGIESLRCDGLRSTLLYDGVPAGLSARPTLSVRIRSPRAGSATVTLSYIASGFDW
                     QADYVARLSPDGSKMDLFAWLTLANGDETGFREADTQAVAGRINRSSQAPARPQAPPL
                     VLSCWPAGTTTSDIEEQVFEKEEIMVTGSRAMMEMMMPLPEPAPAPPPPPAMMAQQEE
                     LGDLKLYRIPEPVTVAARSQKQVALLAREGVKVETVHRVDVYAPGPGGLRVNRILTTR
                     NTRAEGLGLPLPAGRVVLFGTGHARPILLGQGSVDDHAVGEEVEIVVGAATSVQAQAV
                     RISSGPDQAGEWELSVSNARPAPVRFEAQLHEGGMKLTSNTALGRRDGRPLWSVTIPA
                     NGRATLRYRLGKP"
     gene            complement(1216..1608)
                     /locus_tag="VGB57_07815"
     CDS             complement(1216..1608)
                     /locus_tag="VGB57_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0131296.1"
                     /translation="MSGERLGEERREPILRVVPRPGDINSNGHIFGGWVLSQMDIAAG
                     IVAARESDGPVATVAVDAMKFLAPILLHDLISVYAWVERIGRTSIAIRIDVIAIRDRG
                     ASEVKVTEGLFTFVALDENNRPRPVKRG"
     gene            complement(1605..1901)
                     /locus_tag="VGB57_07820"
     CDS             complement(1605..1901)
                     /locus_tag="VGB57_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131297.1"
                     /translation="MQSMPHHDPIPPKPEASALAWSRYKSMMKWMALASAIAAAIAVA
                     WLKATSDAMPWQMVLATIAGVGLSVLLGTALMGLVFLSDRTGHDDAASGREERS"
     gene            1962..2390
                     /locus_tag="VGB57_07825"
     CDS             1962..2390
                     /locus_tag="VGB57_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0131298.1"
                     /translation="MTIAAILDGKPRDIVSVEAGTSVRDAVALLAEKKIGAMPVLDGE
                     RVVGIMSERDVIYCLSSDGADMLDWPVSRVMTAPAITIESKVSVLAALSQMTKRRIRH
                     LPVVERERLIGFVSIGDLVAYRISMIEREAEAMRAYIQGA"
BASE COUNT          523 a         1034 c         1040 g          516 t
ORIGIN      
        1 gctctcgccg ggaagcctgc tcgatgcctc gctcggccgc cgagtgcacc tgcgccgcac
       61 ttcgcgggcg accggcaagg tgcgcgagac ggaagcggtg gtccgctcgg gcgccgccgg
      121 cgcggtcgtg ctggagaccc ctgacgggat cgaatccctg cgctgcgacg gccttcgctc
      181 caccctgctc tacgacgggg tgcccgcggg cctttccgcc aggccgaccc tctccgtccg
      241 aatccgcagc ccgcgcgcgg ggagcgcgac cgtcaccctc tcctacattg ccagcggctt
      301 cgactggcag gccgattatg tcgccaggct ctcccccgac ggctcgaaga tggacctgtt
      361 cgcctggctc accctcgcca atggcgacga gaccggcttc cgcgaggccg acacccaggc
      421 ggtcgccggt cggatcaacc gaagctccca ggctccggcc aggccgcagg cgccgccgct
      481 cgtgctcagc tgctggcccg ccggcaccac caccagcgac atcgaggagc aagtcttcga
      541 gaaggaggag atcatggtca ccggctcgcg ggccatgatg gagatgatga tgccgttgcc
      601 ggagcccgcc ccggctccgc cgccgccgcc cgcgatgatg gcgcagcagg aggagcttgg
      661 cgatctcaag ctctaccgga tcccggaacc ggtcacggtc gccgcccgaa gccagaagca
      721 ggtcgcgctg ctcgctcggg agggagtgaa ggtcgagacg gtccaccggg tcgacgtcta
      781 cgcccccggc cccggcggcc tccgggtcaa ccgaatcctc accactcgca atacccgcgc
      841 ggaaggactc ggcctgccgc tgccggccgg gcgggtggtt ctgttcggca ccgggcacgc
      901 acgcccgatc ctgctggggc aaggctcggt cgacgatcat gccgttggcg aggaagtcga
      961 gatcgtggtc ggcgccgcca cgtcggtgca ggcgcaggcg gtgaggatct cgtcgggacc
     1021 ggatcaggcc ggggaatggg agctctcggt gagcaacgcc cggccggcgc ctgtgcgatt
     1081 cgaggctcag ttgcacgagg gcgggatgaa gctgacctcg aacaccgccc tcggccgccg
     1141 cgacgggcgc ccgctgtggt cggtgaccat ccctgccaac ggtcgggcca ccctgcgcta
     1201 ccgcctcggc aaaccctagc cgcgcttcac cggccgcggc cggttgttct cgtcgagggc
     1261 gacgaaggtg aacagcccct ccgtgacctt cacctcgctc gccccgcggt cgcgaatcgc
     1321 gatgacgtcg atccggatgg cgatcgaggt ccggccgatc cgctcgaccc aggcatagac
     1381 cgagatgagg tcgtggagca ggatcggcgc gaggaacttc atcgcgtcga ccgccaccgt
     1441 cgccaccggc ccgtccgatt cgcgcgccgc gacgatcccg gcggcgatgt ccatctggga
     1501 gaggacccag ccgccgaaga tatggccgtt ggagttgatg tcgcccggcc tcgggaccac
     1561 ccgaaggatc ggctcgcgcc gttcctcgcc aagccgttct ccgctcacga cctctcctcc
     1621 cggccggacg cggcgtcgtc atggccggtc cggtcggaaa ggaagacgag gcccatcaag
     1681 gcggtgccga gcagcaccga caggccaacc ccggcgatcg tcgcgagcac catctgccag
     1741 ggcatggcgt cgctcgtcgc cttgagccag gcgacggcga tcgcggcggc gatcgccgag
     1801 gccaaagcca tccacttcat catgctcttg tagcgggacc aggcgagcgc ggacgcttcg
     1861 ggcttgggcg ggatgggatc gtggtggggc atgctttgca tttgggcctc tcccatggga
     1921 tgattcaacc cgagtcgggc gaaaaagagg agtgaaggcc catgaccatc gccgccattc
     1981 tcgacgggaa accgcgcgac atcgtcagcg tcgaggccgg caccagcgtg cgcgacgcgg
     2041 tggccctgct cgccgagaag aagatcggag cgatgccggt gctcgacggc gagcgggtgg
     2101 tcgggatcat gtctgagcgg gacgtcatct actgcctctc gagcgacggc gccgatatgc
     2161 tcgactggcc ggtgagccgg gtgatgacgg cgccggcgat caccatcgaa tcgaaggtgt
     2221 ccgtgctcgc cgctttgtcg caaatgacga agcggcggat ccgccatctc cccgtggtcg
     2281 agcgcgagag gctgatcggc ttcgtctcga tcggcgacct cgtcgcctac cggatcagca
     2341 tgatcgagcg ggaagccgaa gcgatgcgcg cctatatcca gggcgcgtga cacttttttg
     2401 gatattcgtc cgattcgggg ggttgaaagc ccggagaacc ttatctagat cagcccctcg
     2461 tcggcggcgg ccggcacctt cgggagctca agccgctgac atctttgaaa aaagtatcgc
     2521 gagggccttg aaggcgggat cagccccgac taaataggtt ctagaaggcg cggcccggca
     2581 acggatcgcg gtttttcgga aaccctccag cttcgcatcg gtgaccggct caggccgctc
     2641 tccggcgcga cggcgacgcc cggatctcgg tccggccagc cgaacagaag ttggaaagtt
     2701 tccgaaaaaa gccattgacg gaggcaggca gcctccatat atggcccttc accgacgggg
     2761 cggttttgcc cgacccgtct gcgtcttctc aatcggtagc ccggttgccc cgtaaaaggg
     2821 gagaggaaaa cgcggctctt tgacattgtc ggtttagatg aagggacatg tgggcggcgg
     2881 ccccggtcag cgacagcctc tgggtgtcgt tggtcggtta aagctaagcc gttccaaagg
     2941 tctttcctat gaagcttgct tcagggatcg accctacatg tctcgttaca tatccacagt
     3001 atatgtgaag tgcaggaacg gctcccagag atgccggcct cttcctcggg ttttcccccg
     3061 gggtggttgt cggtcattaa cttgagagtt tgattctggc tcagaacgaa cgc
//
LOCUS       DASWNU010000392         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_483034, whole genome shotgun sequence.
ACCESSION   DASWNU010000392 DASWNU010000000
VERSION     DASWNU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483034"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VGB57_07830"
     CDS             complement(<1..577)
                     /locus_tag="VGB57_07830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY0131299.1"
                     /translation="MSFELKLSVSPTARAMRLRVDRRTGEVVLTIPKRASQRKALAWA
                     DGHRAWIEARLAEVEPALPLAPGTDIPLYGRPHRIDWSPERPRTPRLEEGRLVAGGPE
                     EGLEARLLRWLRRHALDTLAAETAEFAAKAGVKVSRIGIGDPVSRWGSCSATGGIRYS
                     WRLILAPDRVRRATVAHEVAHRVHMNHGPEFH"
     gene            complement(567..992)
                     /locus_tag="VGB57_07835"
     CDS             complement(567..992)
                     /locus_tag="VGB57_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEY0131300.1"
                     /translation="MNFWEKPLAALDRGEWEALCDGCGKCCLHKLEDEDSGEIHATNV
                     ACRLLDRHSGRCTNYRGRKAYVPDCVRLTSSKVGALAWLPSTCAYRLRSEGKPLPDWH
                     YLVCGDREAVHRAGMSIRGWTVAEDEAGDLEHHLVEREL"
     gene            complement(989..1588)
                     /locus_tag="VGB57_07840"
     CDS             complement(989..1588)
                     /locus_tag="VGB57_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0131301.1"
                     /translation="MNETRLNRRRPLLFLALALALAACSGAGEEPPLKGAAIGGPFTL
                     TNQDGRQVTERDFAGRYRLIYFGFTHCPDVCPTDLAVIGQALRRFEESEPERAARVAP
                     IFVSVDPQRDSPAVLKEYVSAFHPRLIGLTGTPKQIADMTKSYGAYGAKDPAGPGGGY
                     NVNHSRLAELIGPDGKPIALLPYEKGAEAVAAELERWVK"
     gene            1648..>1958
                     /locus_tag="VGB57_07845"
     CDS             1648..>1958
                     /locus_tag="VGB57_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0131302.1"
                     /translation="MFNKFGRALAASMVLALAVPASGQQTFSDSFTFLKAVRERDGDK
                     VSDLVAQPGSVVINTRDRSNGEGALHIVTRGRDLSWLTFLLLKGARPDLQSSRGDTPL
                     TL"
BASE COUNT          287 a          679 c          685 g          307 t
ORIGIN      
        1 cgtggaattc gggaccgtgg ttcatatgga cccggtgggc gacttcgtgg gcgaccgtcg
       61 cccgcctcac ccggtcggga gcgaggatga ggcgccagct atagcggatc ccgcccgtcg
      121 ccgagcaact cccccagcgc gacacagggt cgccgatccc gatccggctg accttcacgc
      181 ccgccttggc cgcgaactcc gcggtctccg cggcgagcgt gtcgagcgcg tgccgcctca
      241 gccagcgcag cagccgcgcc tcgagccctt cctccggccc cccggcgacc aggcgcccct
      301 cctccagcct cggcgttcgc ggccgctcgg ggctccagtc gatccggtgc ggccggccgt
      361 agaggggaat gtcggtcccg ggcgcgagcg gcagggccgg ctcgacctcg gccagccggg
      421 cctcgatcca tgcgcggtgg ccgtccgccc aggccaaagc cttgcgttgc gacgcccgtt
      481 tgggaatggt caggaccacc tcgcccgtgc gccggtcgac ccgaagccgc atcgcccggg
      541 ccgtcggcga gacgctcagc ttcagctcaa agctcacgct cgaccagatg atgctcgaga
      601 tcgcccgctt cgtcctcggc caccgtccag ccgcggatcg acattccggc gcggtgcacg
      661 gcctcgcggt cgccgcagac gagataatgc cagtcgggca gcggcttgcc ctccgagcgc
      721 aggcggtagg cgcaggtcga cggcagccag gcgagcgctc cgaccttcga ggaggtcagc
      781 cggacgcaat cgggcacata ggccttgcgc ccgcggtaat tggtgcagcg gccgctgtga
      841 cggtcgagca gccggcaggc gacgttggtg gcgtggatct caccgctgtc ctcgtcctcg
      901 agcttgtgca ggcagcattt gccgcagccg tcgcacaagg cctcccattc gccgcggtcg
      961 agggccgcaa gtggcttctc ccagaaattc acttcaccca ccgctcgagc tcggcggcga
     1021 ccgcttcggc gcctttctcg taaggaagga gggcgatggg cttgccgtcg gggccgatca
     1081 gctccgcgag ccggctgtgg ttgacgttgt agccgccgcc cgggcccgcg gggtctttgg
     1141 ctccataggc gccgtagctt ttggtcatgt cggcgatctg cttcggggtt ccggtgaggc
     1201 cgatcaggcg ggggtggaag gctgagacat attccttgag caccgccggc gaatcgcgct
     1261 gcggatcgac gctgacgaag atcggcgcga cccgagcggc gcgctcgggt tcgctctcct
     1321 cgaatcggcg gagcgcctgg ccgatgacgg cgagatcggt cgggcagacg tcgggacaat
     1381 gggtgaagcc gaaatatatg agccggtagc ggccggcgaa atcccgctcg gtcacctggc
     1441 gcccgtcctg attggtcagg gtgaagggcc cgccgatcgc ggcgcccttg agcggcggct
     1501 cctcgccggc gcccgagcag gcggccaggg cgagcgccag cgcaaggaaa aggagcggcc
     1561 gacggcggtt caagcgggtt tcgttcatgg cggaagcagc cttgtcttgc tatagccgcg
     1621 ccgacaagtc gagacgggga agcgagagtg ttcaataagt tcggccgcgc gctggcggcg
     1681 tccatggtcc tggcgctcgc ggtcccggcg tcggggcagc agactttctc cgacagcttc
     1741 accttcctca aggcggtcag ggagcgcgac ggcgacaagg tgagcgatct ggtggcgcag
     1801 cccggttcgg tcgtcatcaa cacccgcgac cggagcaacg gcgaaggcgc gctccacatc
     1861 gtcacccgcg gccgcgacct ttcctggctt actttcctcc tcctcaaggg cgcccggccg
     1921 gacttgcaga gcagccgcgg cgacacgccg ctgactct
//
LOCUS       DASWNU010000393         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_377842, whole genome shotgun sequence.
ACCESSION   DASWNU010000393 DASWNU010000000
VERSION     DASWNU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377842"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VGB57_07850"
     CDS             complement(<1..349)
                     /locus_tag="VGB57_07850"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEY0131303.1"
                     /translation="MATNPTGTRVPLIQRVKNILTTPKTEWPVIDSEPDTIGGIYKNY
                     VMILAAIPPVCMLLGLLVFGMPYFAFPIGYLLAQAVISYVMALIGCYILALIIDALAP
                     TFGGTKDKVKAFKV"
     gene            479..>1667
                     /locus_tag="VGB57_07855"
     CDS             479..>1667
                     /locus_tag="VGB57_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEY0131304.1"
                     /translation="MISATLLALAAAASPAPKEVVAFPGAEGAGRLALGGRGGRVIRV
                     TSLEDSGPGSLRAAIEAKGPRTIVFDVGGTIRLKKPLVVRSGRVTVAGQTAPGGGITV
                     RDRVFEIAADDVVVRYVRARLGDESRVESDAFTISRGRRIVVDHVSASWSVDETLSAG
                     SPYKGPEDDLRDLTVQWSIISESMRKSVHAKGEHGYGSLVRGGHGAKMSFHHNLWAHH
                     AARMPRPGNYLTPAQDSVGAYYDFRSNVFYNWGGSHSGYNADSGEKASHAAYNFVDNY
                     YLRGPDSKKPVAFDERNPIARAWFAGNAMDGAIPKDPWSLVTGRTDREYRLAGPLAVG
                     KVERDPALRAYERVLAGAGASRVRDAVDLRVVASVRSRGGGQIDSQADVGGWPELAAG
                     SPWI"
BASE COUNT          278 a          533 c          585 g          271 t
ORIGIN      
        1 cgaccttgaa ggccttcacc ttgtccttgg tgccgccgaa ggtcggcgcc agggcgtcga
       61 tgatgagggc cagtatgtag cagccgatca gcgccatcac gtaggagatg accgcttgcg
      121 caaggagata gccgatcgga aaagcgaaat agggcattcc gaaaacgagc agtccgagca
      181 gcatgcaaac cggcgggatt gccgccagga tcatcacata attcttgtag atgccgccga
      241 tcgtgtccgg ctcggaatcg atgaccggcc attcggtctt cggagtcgtc aggatgttct
      301 tgacccgctg gatgagcggc acgcgggttc cggtcggatt ggtcgccatg cttcattccc
      361 ccttgtttcc ggcggaatga agcatggcgc ggtcggaaac tcaaccggca ggcgaagatc
      421 ggcttcccag gcgcatcttg gtcatgacac cggtatcaac ggatgctaag aggctggaat
      481 gattagcgcc accctcctcg ccctcgccgc cgccgcctct cccgctccaa aggaagtggt
      541 ggcctttccc ggggccgagg gggccgggcg gcttgctttg ggcgggcgcg gcgggcgtgt
      601 gatccgggtc accagcctcg aagatagcgg gcccggctcg cttcgggcgg cgatcgaggc
      661 gaaggggccg cgcaccatcg tgttcgacgt cggcggcacg atccggctca agaagccgct
      721 tgtcgtccgc agcggccggg tgacggtggc ggggcagacg gcgccgggcg gcggcatcac
      781 cgttcgcgac cgggtgttcg agatcgccgc ggacgacgtc gtcgtgcgct acgttcgcgc
      841 ccgacttggc gacgagagcc gggtggagag cgacgccttc acgatcagcc gcggccggcg
      901 gatcgtcgtc gaccacgtct cggcgagctg gtcggtcgac gagactcttt cggctgggtc
      961 cccctacaag ggccccgagg acgacctgcg cgacctcacc gtgcaatggt cgatcatctc
     1021 ggaatcgatg aggaagtcgg tgcacgccaa gggcgagcat ggctacggct cgctggtgcg
     1081 cggcggccat ggcgcgaaga tgagcttcca ccacaatctg tgggcccacc acgccgcccg
     1141 catgccgagg ccgggcaatt acctgacccc ggcgcaggac agcgtcggcg cctattacga
     1201 tttccgctcc aacgtcttct acaattgggg cggcagccac agcggctaca atgccgacag
     1261 cggcgagaag gcttcccacg cggcgtataa tttcgtcgac aattactatc tgcgcgggcc
     1321 ggactcgaag aagccggtcg ccttcgacga gcgcaatccg atcgcccgcg cctggttcgc
     1381 cggcaatgcg atggacgggg ccatcccgaa ggatccgtgg agcctcgtca ccggccggac
     1441 cgaccgcgaa tacaggctgg cgggaccgct cgcggtcggg aaggtcgagc gcgacccggc
     1501 gcttcgcgcc tacgagcggg tgctcgccgg cgccggcgcg tcgcgggtcc gtgacgccgt
     1561 ggatctgcgg gtggtcgcga gcgtgcgcag ccgcggcggg gggcagatcg acagccaggc
     1621 cgacgtcggc ggctggcccg agctggccgc tggttcgccc tggatcg
//
LOCUS       DASWNU010000394         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_377890, whole genome shotgun sequence.
ACCESSION   DASWNU010000394 DASWNU010000000
VERSION     DASWNU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377890"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VGB57_07860"
     CDS             complement(<1..274)
                     /locus_tag="VGB57_07860"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0131305.1"
                     /translation="MSRRAMITGVTGQDGSYLAELLLEKGYEVHGLVRRLSHDNHERL
                     FGIRDRITLHSGDLLDQRSLVTAMRAARPDEIYNLDAMSFVGASWTQ"
     gene            complement(320..1276)
                     /locus_tag="VGB57_07865"
     CDS             complement(320..1276)
                     /locus_tag="VGB57_07865"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0131306.1"
                     /translation="MITGITGQDGSYLAELLLEQGYEVHGLVRRPEDGLGPDLADLEQ
                     RLVLHRGDLRDGPSLSAALKEAQPDEVYNLAAVSRVPLSWERAVDSAEANALGVVRLL
                     DEIVAGGCEARFFQASSSEIFGGSGGVPQDESTPLAPRSPYGAAKAYGHLLVDTYRRR
                     YDLFACSGILYNHESPRRPEAFVSRRVTAGAAAVKLGLADDLQLGNLDARRDWGYAPE
                     YMEAAWLMLRAEEPEDFVIGSGRAHSVAELVAVAFAEVGLDSAPYLRRDPVAARPDDL
                     DYPLADPGKARERLGWEPRTGFEELVQIMVRADLERLTGRSG"
     gene            complement(1303..>1799)
                     /locus_tag="VGB57_07870"
     CDS             complement(1303..>1799)
                     /locus_tag="VGB57_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0131307.1"
                     /translation="KGDAVVIVMADGSDNPRDVVLYFRVLEAGYDCAFGSRFMPGATV
                     SDYPRLKLLINRVVNAGIRAIFRHGYNDTTNAFKAYRREVIETIQPLLSKHFNLTVEM
                     PLKAIVRGHSFAVVPTSWTNRTSGEAKLAMKEMGSRYLFIVLYVWLESTLSRGDYQVG
                     EPPA"
BASE COUNT          276 a          595 c          644 g          284 t
ORIGIN      
        1 gctgcgtcca ggacgctccc acgaaggaca tcgcgtcgag gttgtagatc tcgtccggcc
       61 gggccgcccg catcgcggtg accagggagc gctggtcgag caggtcgccg ctgtgcagcg
      121 tgatccgatc ccggatcccg aagagccgct cgtggttgtc gtgagaaagg cggcgaacga
      181 ggccgtggac ctcgtagccc ttttcgagca gcagttcagc caggtagctg ccgtcctggc
      241 cggtgacacc ggtgatcatc gcccgcctag acatctctcc ctccccgaga gtacgacagt
      301 tacaaaagct tggcgaactt tagccgctac gtccggtcag gcgttccaaa tcggccctga
      361 ccatgatctg gaccagctcc tcgaaaccgg tgcggggctc ccagcccagc ctctcccgcg
      421 ccttgccggg atcggcgagg ggatagtcga ggtcgtcggg cctcgccgcg acggggtcgc
      481 ggcgcaggta gggggcggaa tccagcccca cctccgcaaa ggccacggcg accagctcgg
      541 ccaccgaatg ggcccggccg ctgccgatga cgaagtcctc cggctcctcg gcccgcaaca
      601 tcagccaggc ggcttccatg tactcgggcg catagcccca gtcgcgcctg gcgtccaagt
      661 tgccgagttg gaggtcgtcg gccaggccga gcttgacggc cgccgccccg gcggtcaccc
      721 ggcgcgagac gaacgcctcg ggccggcggg ggctctcgtg gttgtagagg atcccggagc
      781 aggcgaagag gtcgtagcgg cgccgatagg tgtcgacgag caggtggccg taggccttcg
      841 ccgccccgta gggggagcgg ggcgcgagcg gcgtcgactc gtcctggggg actccgccgg
      901 agcccccgaa gatctcactc gaggaggcct ggaagaagcg cgcttcgcag ccgccggcga
      961 cgatctcgtc gagcaggcgg acgaccccga gggcgttggc ctcggcgctg tcgaccgccc
     1021 gctcccagga gagcgggacc cggctcaccg cggcgaggtt gtagacctcg tcgggctgcg
     1081 cttccttcag cgccgccgac agcgagggcc cgtcgcgcag gtcgccgcga tgcagcacca
     1141 ggcgctgttc gagatccgcg aggtcgggcc cgaggccgtc ctccgggcgg cggacgaggc
     1201 cgtggacctc gtagccctgc tccagcagca gctcagccag gtagcttccg tcctggcccg
     1261 tgataccggt gatcatggct cttctggcca cggttgggcg gcttatgcgg gcggctcgcc
     1321 gacctggtaa tcgccgcggc tgagggtgct ctcgagccag acgtagagga cgatgaagag
     1381 gtagcggctg cccatctctt tcatcgccag cttcgcctcg ccgctggtgc ggttggtcca
     1441 ggaggtgggg acgacggcga agctgtggcc gcggacgatc gccttcagcg gcatctcgac
     1501 ggtgaggttg aagtgtttgg agagcagcgg ctggatcgtc tcgatcacct cgcgccggta
     1561 ggccttgaag gcgttggtgg tgtcgttgta gccgtggcgg aagatcgcgc ggatgccggc
     1621 gttgaccacg cgattgatga gcagcttcag gcgcgggtag tcgctcaccg tcgcgcccgg
     1681 catgaagcgc gagccgaacg cgcagtcata gcccgcctcg agcacgcgga agtacaggac
     1741 gacgtcgcgc gggttgtccg agccgtcggc catgacgatg accaccgcgt cgcccttga
//
LOCUS       DASWNU010000395         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_169455, whole genome shotgun sequence.
ACCESSION   DASWNU010000395 DASWNU010000000
VERSION     DASWNU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169455"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..549)
                     /gene="rplQ"
                     /locus_tag="VGB57_07875"
     CDS             complement(127..549)
                     /gene="rplQ"
                     /locus_tag="VGB57_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY0131308.1"
                     /translation="MRHRNAGRKLQRTSSHRAALFRNMAAALIKHEQITTTVAKAKEL
                     RPYTEKLITLAKKGGLSNRRLAHARLLDDTQLQKLFDVLAPRYAGREGGYTRVVKAGI
                     RASDAAPIAIIELVDRDVSAKGQDSGPVMSDEYEAEAA"
     gene            795..1301
                     /locus_tag="VGB57_07880"
     CDS             795..1301
                     /locus_tag="VGB57_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131309.1"
                     /translation="MRIILSLAAGVGAMAITAAAPAKPGPEAFNGGGGQPGWSRGGGE
                     NAGGGLYFRHAPSGIRGYRGQGRQGRHGRFGRHGRDGYSSYGVFGIGGPAGAVDPWGN
                     GFFNGGGGRIRLHGDRPHYDYDRSYPYEWAPAAGGGEDREDAAGPGASERTPRCTFEN
                     GVRVCRGW"
     gene            complement(1318..2493)
                     /locus_tag="VGB57_07885"
     CDS             complement(1318..2493)
                     /locus_tag="VGB57_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0131310.1"
                     /translation="MPEAYIVAAARTAGGRRGGALRDWHPADMAAEILDSLIHRSGLD
                     PEAVEDVIMGCVGQAGEQAFHIGRNAVLASSLPTSVPAVTIDRQCGSSQQAVQFAAQA
                     VMSGTQDVVIAAGVESMSRVPMGLPVTLPMQHGIGTGPFSKRIQDRFGVPMFSQFVGA
                     EMIAEKYQFRKEELDAFALDSHLKAARATGAGAFDEEIVPLRIVDSEGNETLHTKDEG
                     IRADSTLESISSVKLLKEGGKITAANASQICDGASGVIVVSERALKEHNLTPLARIVN
                     LTVTGGDPVIMLEEVIPATRRALERSGMSIADIDLYEVNEAFAPVPLAWLREVGADPA
                     RLNVNGGAIALGHPLGASGTKLMTTLVHALRAKGRRFGLQTMCEGGGLANVTIVEAL"
BASE COUNT          356 a          817 c         1012 g          421 t
ORIGIN      
        1 gtcggggagg ttccgtctgt agccgtcatt gcgaggagcg cagcgacgcg taatccagcg
       61 ggcctcgaag gctgcactgg attgcttcgc ttcgctcgca atgacgggtg ggcgagcggc
      121 ccgtcgttac gcagcttcgg cttcgtactc gtcgctcatc accgggcccg aatcctggcc
      181 cttggccgag acgtcgcggt cgaccagctc gatgatggcg atcggggcgg cgtcggaggc
      241 tcggatgccg gccttgacga ccctcgtgta gccgccctcg cggcccgcgt agcgcggcgc
      301 caggacgtcg aagagcttct ggagctgggt gtcgtcgagc agtcgggcat gggcgaggcg
      361 gcggttggag aggccgccct tcttggcgag cgtgatcagc ttctccgtat agggacgaag
      421 ctccttcgcc ttggcgacgg tggtggtgat ctgctcgtgc ttgatgagcg cggcggccat
      481 gttgcggaac agggcggctc ggtggctcga ggtacgctgc agcttgcggc cggcgttgcg
      541 gtgacgcatg tcttcatcct tcgttcgtcc ggcgcccgtt tcaggtagca ccggccgggc
      601 ggccatgtgg gagaccgccc aaatcccaaa atgcgacggc ggggccgaag agcctcgccc
      661 gcgggggtcc ctcaagccga gacgcggcgt aaagtcaagg ctgcgggcgt gcgcaggagc
      721 gattcggcat gccgagggtt gacgtcatgg cgccgcggcg cccggccgcg gagcgtccaa
      781 caaggagggc gaggatgcgc atcatcttat ctctggcggc gggcgtcggt gcgatggcga
      841 tcacggcggc ggcgccggcg aagcccgggc cggaggcctt caacggcgga ggaggccagc
      901 ccggctggtc gaggggcgga ggcgagaatg cgggcggcgg cctctatttc cgccacgccc
      961 cgagcgggat caggggctat cgcgggcaag gccgccaggg ccgccacggc cggttcgggc
     1021 gccatgggcg ggacggctat tcctcctatg gcgtcttcgg tatcggcgga ccggccgggg
     1081 cggtcgatcc ctggggcaac ggtttcttca acggcggcgg cgggcggatc cgattgcacg
     1141 gcgaccggcc gcattacgat tacgaccgct cctaccctta tgaatgggct cccgccgccg
     1201 gcggtggcga ggatcgggag gacgcggccg ggccgggcgc gtcggagcgg acgccgcgct
     1261 gcacgttcga gaacggagtc cgggtctgcc ggggctggtg aggccgggcc tccgggctca
     1321 gagggcttcc acgatcgtca cattggccag cccgccgccc tcgcacatgg tctggaggcc
     1381 gaagcgcctg cccttcgccc tgagggcgtg gacgagggtg gtcatgagct tggtgccgga
     1441 ggcgccgagc gggtggccga gcgcgatggc gccgccgttg acgttcagcc gggccgggtc
     1501 ggcgcccacc tcgcgcagcc aggccaaagg gacgggcgcg aaggcttcgt tgacttcgta
     1561 gaggtcgatg tcggcgatgc tcatgcccga gcgttcgagg gcccggcggg tggcggggat
     1621 gacttcctcg agcatgatga cggggtcgcc gccggtcacc gtcaggttga cgatgcgggc
     1681 cagcggggtg aggttgtgct ccttcagcgc ccgctccgac acgacgatca cccctgaggc
     1741 cccgtcgcag atctgtgagg cgttggcggc ggtgatcttg ccgccttcct tgagcagctt
     1801 caccgaggag atgctctcca gggtggaatc ggcgcggatg ccctcatcct tcgtgtggag
     1861 ggtctcgttg ccctcggagt cgacgatcct gagcgggacg atctcctcgt cgaaggcgcc
     1921 ggccccggtg gcccgggcgg ccttcaagtg gctgtcgagg gcgaaggcgt ccagctcctc
     1981 cttccggaac tggtatttct cggcgatcat ctcggcgccg acgaactggc tgaacatcgg
     2041 caccccgaag cggtcctgga tccgcttcga aaaggggccg gtgccgatcc cgtgctgcat
     2101 cgggagggtg acggggaggc ccatcggaac ccggctcatc gactcgacgc cggcggcgat
     2161 caccacgtcc tgggtgccgc tcatcaccgc ctgggcggcg aattgcaccg cctgctggga
     2221 ggagccgcac tggcggtcga tggtgacggc cgggaccgag gtcgggaggg acgaggcgag
     2281 gaccgcgttg cggccgatgt ggaaggcctg ctcgccggcc tggccgacgc agcccatgat
     2341 cacgtcctcg acggcctcgg ggtcgagccc ggagcggtgg atgagcgagt cgaggatctc
     2401 ggcggccatg tcggcggggt gccagtcgcg aagggctccg cccctgcgcc cgccggcggt
     2461 gcgggcggcg gcgacgatat aggcttcggg catggaaggg ctcctgtcgt tcggggcgat
     2521 gtaggggcgc gggggagggt ggcgcaaggt cgtcatcccg gcggaagccg ggatctcggc
     2581 gagacccgcc gggacttctc gtcccg
//
LOCUS       DASWNU010000396         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_455213, whole genome shotgun sequence.
ACCESSION   DASWNU010000396 DASWNU010000000
VERSION     DASWNU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455213"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VGB57_07890"
     CDS             complement(<1..454)
                     /locus_tag="VGB57_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0131311.1"
                     /translation="MATAGSPEVEKARATVRLQQLSEGVGRIANVLAALSEHEAAAAA
                     MAAVGAVPNGEGDALDAGFIRSIIRVRRLRDHFFKSDLFADPAWDMLLDLMAARVERQ
                     RVAVSSLCIAAAVPPTTALRWIKGLCDQGLFVRVADPEDGRRVFIELSG"
     gene            complement(698..1393)
                     /locus_tag="VGB57_07895"
     CDS             complement(698..1393)
                     /locus_tag="VGB57_07895"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEY0131312.1"
                     /translation="MRKLGMIGGTSWFSTAMYYEQINKAVARAKGGLSSAPLVIESLD
                     LAPVAALELAGEWDKVGEIVAAAAARLEAAGAEGLILCSNTTHKVAPAVEAAVKVPLL
                     HIADVTAERLAADGVQKAGLIGTRFTMGEGFFRDRIAARGIALALPDPATAEQIDRII
                     FEELARGQVSRNSQRLLKTCVTEMGKARQQAVILGCTELVLLVDPGANVIPVYDTTAL
                     HAAAAARWILEEV"
     gene            complement(1587..>2580)
                     /locus_tag="VGB57_07900"
     CDS             complement(1587..>2580)
                     /locus_tag="VGB57_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY0131313.1"
                     /translation="GAVTFSGSVIAFLKLNGNMSGKPILLPLRHVINLAILAAIVGLV
                     GYFTTDQSAWIFVALTALAFLVGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTLQ
                     NSAMIITGALVGSSGAILSYIMCRAMNRSFISVIAGGFGGDTAIAGGGAASDRPWKRG
                     SAEDAAFLMSQAEQVIIVPGYGMAVAQAQHAVRELADQLKKEGVTVKYAIHPVAGRMP
                     GHMNVLLAEANVPYDEVFELEDINSEFARTDIAFVIGANDVTNPAAKTDKTSPIYGMP
                     VLDVEKAKTVLFIKRSMGGVGYAGVDNDLFYRDNTMMLLGDAKKMTEEIVKSLG"
BASE COUNT          426 a          886 c          829 g          439 t
ORIGIN      
        1 cgccggacag ctcgatgaac acgcgccggc cgtcctccgg gtcagcgacc cggacgaaca
       61 ggccctggtc gcacaggccc ttgatccatc gcagcgccgt cgtcggcggt accgccgcgg
      121 cgatgcacag gctcgacacg gcgacccgct gcctttccac ccgcgccgcc atcaggtcga
      181 gaagcatgtc ccaggcggga tcggcgaaca ggtcgctctt gaagaaatgg tcgcgaagcc
      241 gcctcacccg gatgatcgag cggatgaagc cggcgtcgag ggcgtcgccc tcgccgttcg
      301 gaaccgcgcc gaccgccgcc atcgcggctg ccgccgcctc atgctcggaa agggccgcga
      361 gcacgttcgc gatccgcccg actccctcac tcagttgctg cagccgcacc gtcgcccggg
      421 ccttttcgac ctcgggactc cctgccgtcg ccatcgtctc cccgatctgc ccgccagcgc
      481 cggagattgc cgccgttccg gagggattgc aggcgcacat tgatccattt tggaggagaa
      541 tggccgccag attgcgcgct aaatccggaa gacgaatatc ccggccgagc ctgaactctg
      601 cccgcgaccc ggaaacaggg cggacagggc ggcggctgga actgaggtta gtctccgaaa
      661 tgcgcctatc ctgtcggcac acttcctcca tttcggatta aacttcctcc aatatccagc
      721 gagcggcggc ggcggcgtgg agcgcggtcg tgtcgtagac cgggatgacg ttggcgccgg
      781 ggtcgacgag aagcaccaat tcggtgcagc cgaggatcac cgcctgttgc cgcgccttgc
      841 ccatctcggt gacgcaggtc ttgagcagcc gctgcgaatt gcggctcacc tgccccctcg
      901 ccaactcctc gaagatgatc cggtcgatct gctcggccgt cgccggatcg ggcagagcga
      961 gcgcaattcc ccgcgccgcg atccgatcgc ggaagaagcc ctcgcccatg gtgaagcggg
     1021 tcccgatcag gccggccttc tggactccgt cggcggcgag acgttcggcg gtgacgtcgg
     1081 cgatgtggag gagcggcacc ttgaccgccg cctcgaccgc gggcgccacc ttgtgggtgg
     1141 tgttgctgca caggatcagc ccttcggccc ctgccgcctc gagccgagcc gcggcggcgg
     1201 ccacgatctc gcccaccttg tcccattcgc cggcgagctc gagcgcggcg accggcgcca
     1261 gatcgaggct ttcgatgacc agcggcgccg agctcaggcc gcccttcgcc ctcgccaccg
     1321 ccttgttgat ctgctcgtaa tacatggcgg tcgagaacca gctcgtcccg ccgatcattc
     1381 ctagtttgcg cattcggcga cccatagccg cgaaggccgg ggaggaaagg gctttcggcc
     1441 catcgacttc acttatgcgc cgaagcgcgc agttcccccg tcattgcgag gagcgtagcg
     1501 acgaagcaat ccagcgggcg tcggaggccc ctctggattg cttcgcttcg ctcgcaatga
     1561 cggggtcggt cgctccggcc ggaaacctac cccagcgact tcacgatctc ttcggtcatc
     1621 ttcttggcgt cgcccagaag catcatcgtg ttgtcgcggt agaagaggtc gttgtcgacg
     1681 cccgcatagc cgaccccgcc catcgagcgc ttgatgaaca ggacggtctt ggccttctcc
     1741 acgtcgagca ccggcattcc gtagatgggc gaggtcttgt cggtcttggc ggccgggttg
     1801 gtgacgtcgt tggcgccgat gacgaaggcg atgtcggtcc tggcgaactc ggaattgatg
     1861 tcctcgagtt cgaacacctc gtcatagggg acgttggcct cggcgagcag cacgttcatg
     1921 tgccccggca tccggcccgc caccgggtgg atggcgtatt tgacggtgac gccttccttc
     1981 ttgagctggt cggcgagctc gcgaaccgcg tgctgggcct gggccaccgc catcccgtag
     2041 cccgggacga tgatcacctg ctcggcctgg ctcatcagga aggccgcgtc ctccgccgat
     2101 ccccgcttcc agggccggtc ggacgctgcg ccgccgccgg cgatcgccgt atcgccgccg
     2161 aagcctccgg cgatgaccga gatgaagctc cggttcatcg cccggcacat gatgtaggag
     2221 aggatcgcgc ccgacgatcc gaccagagct ccggtgatga tcatcgcgct gttctggagc
     2281 gtgaagccca tcgccgccgc cgcccagccc gaatagctgt tcagcatgga gaccacgacc
     2341 ggcatgtccg ccccgccgat cggaatgatc agaaggaagc cgacgaggaa ggcgagcgcg
     2401 gtcagggcca cgaatatcca ggcgctctgg tcggtcgtga aatagccgac caggccgacg
     2461 atcgcggcca ggatcgcgag gttgatgacg tgcctcaggg gcagcaggat cggcttgccc
     2521 gacatgttgc cgttcaattt caggaaagcg atcaccgagc ccgagaaggt caccgccccg
//
LOCUS       DASWNU010000397         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_169517, whole genome shotgun sequence.
ACCESSION   DASWNU010000397 DASWNU010000000
VERSION     DASWNU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169517"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..629)
                     /locus_tag="VGB57_07905"
     CDS             complement(51..629)
                     /locus_tag="VGB57_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131314.1"
                     /translation="MKSVCFALCIAAAAPALASAAPPAAAPVARGEDLDAEFQAMTQP
                     FMDFLNLITRRFPERECVRDRSLLNAEYGKIELSLQQMFVVRDDLRKDVREIAMRLAT
                     FQPLPAELRHRDIPLALPPKTESEKAMIARVVQARTALWKKLDALESRFPASAAALAS
                     KEMGFIITVDDRGWRPGSPGQVLNERKQCGSD"
     gene            complement(626..1918)
                     /gene="nuoF"
                     /locus_tag="VGB57_07910"
     CDS             complement(626..1918)
                     /gene="nuoF"
                     /locus_tag="VGB57_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY0131315.1"
                     /translation="MLEDKDRIFTNLYGFQDWGLKGAQARGDWDDTKTLMAIGEDAII
                     DEIKNSGLRGRGGAGFPTGMKWSFMPKAPTPGRPNFLVINADESEPGSCKDREIIRHD
                     PHKLIEGALIAGFAMRARAGYIYIRGEFIREAQTLEAAVQQAYDAGLLGKNAAKSGYD
                     FDLFVHRGAGAYICGEETAMLESLEGKKGMPRLKPPFPAGAGLYGCPTTVNNVESIAV
                     VPTILRRGASWFKGIGREKNEGTKLFQISGHVNKPCVVEDAMSVPFRELIDRHAGGIR
                     GGWDNLLAVIPGGSSVPLVPAEQIVDAPMDFDGLKDLGSGLGTAAIIVMDKSTDVVKA
                     ISRISYFYKHESCGQCTPCREGTGWMWRVMERLVEGNAEIHEIDTLWDVTKQVEGHTI
                     CALGDAAAWPIQGLIRHFRPELERRIAERRGRTLEAAE"
     gene            complement(1918..2058)
                     /locus_tag="VGB57_07915"
     CDS             complement(1918..2058)
                     /locus_tag="VGB57_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131316.1"
                     /translation="MEQLLPIVVGVIALVIALKVLKGMIKMVALVVIVALVAALYFGM
                     GS"
     gene            complement(2060..2407)
                     /locus_tag="VGB57_07920"
     CDS             complement(2060..2407)
                     /locus_tag="VGB57_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131317.1"
                     /translation="MISIGRFDLLGCTASAFAQDKAGQTLFLPLGKAGSAYAAQDPAR
                     MRARIWGVRLFCAAATLILLGWLALLASQRIDEDPGRWRFGYALGAALIVWFLAYGAW
                     AWLATRGLEKVGG"
     gene            complement(2404..3093)
                     /locus_tag="VGB57_07925"
     CDS             complement(2404..3093)
                     /locus_tag="VGB57_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY0131318.1"
                     /translation="MADILPTSIDLDEVRARWGAFAFTRENDRKAKLLVDRYPPGRQQ
                     SAIIPLLDLAQRQVGAETDTQGWLPIPVIEYVAAYLGMPYIRAYEVATFYTMFNLRPV
                     GRFHVQVCGTTPCMLRGSDDVFAACKAKGMKKGQTTADGLFTLSEVECLGACANAPMV
                     QINDDNYEDLTFETTSAILEALARGETPRPGPQGVNRRTSDPEGGVTSLPEMVGENYD
                     YRSRWGGERPA"
     gene            complement(3086..3310)
                     /locus_tag="VGB57_07930"
     CDS             complement(3086..3310)
                     /locus_tag="VGB57_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131319.1"
                     /translation="MADRRTLAMLALTVAVVAVGALYLFTPDDRADGTAVRTVGGIAA
                     ALVLLLLVALASGSSRGKRPTRDDDGRPDG"
     gene            complement(3303..3575)
                     /locus_tag="VGB57_07935"
     CDS             complement(3303..3575)
                     /locus_tag="VGB57_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131320.1"
                     /translation="MSQILLLAMFVLAPATVFAQLPVKARRYFLAAAVTALLAYAVLM
                     GPALGGGAFLVLPLALMGIIAAALLVEAVALARRLLGRKGRPAAHG"
     gene            complement(3572..>3922)
                     /locus_tag="VGB57_07940"
     CDS             complement(3572..>3922)
                     /locus_tag="VGB57_07940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127254.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY0131321.1"
                     /translation="FEQMPQGPIASLDRKVVPPKRAEMKQSMEALIHHFKLYTEGFHV
                     PAGEVYVATESPKGEFGIYLVADGTNKPYRVKIRPTGFSHLQAMDFMTRGHMLADVTA
                     ILGSLDIVFGEIDR"
BASE COUNT          646 a         1287 c         1348 g          641 t
ORIGIN      
        1 ggcgccctgc ggtacctcga cgtcaatgcc atcgactttg actttaggca tcagtccgac
       61 ccacattgtt tgcgttcatt gagaacctgg cctggactgc ccggccgcca gccgcgatcg
      121 tccacagtga tgatgaagcc catctccttg ctggccaaag cggcggcgct cgccggaaat
      181 ctggattcga gcgcgtccag cttcttccag agcgccgtcc gggcctggac gaccctggcg
      241 atcatcgcct tctcgctttc ggtcttcggg ggaagagcga gggggatgtc gcggtgacga
      301 agctcggcgg gaagcggctg gaaggtggcg aggcgcatgg cgatctcgcg aacgtccttg
      361 cgcaggtcgt cgcggaccac gaacatctgc tgaagcgaca gctcgatctt gccgtattcg
      421 gcgttgagga ggcttcggtc gcgaacgcac tcccgctccg ggaaccgccg ggtgatcagg
      481 ttgaggaagt ccatgaacgg ctgcgtcatg gcctggaatt cggcgtcgag atcctcgccc
      541 cgggcgaccg gcgcggctgc cggcggggcc gccgaagcca aagcgggcgc cgcggcggcg
      601 atgcagagcg cgaaacacac ggacttcatt ccgccgcctc cagggtccgg ccccggcgct
      661 cggcgatccg ccgctcgagc tccggccgga aatggcggat caggccctgg atgggccagg
      721 cggcggcgtc gcccagggcg cagatggtgt ggccctcgac ctgcttggtg acgtcccaga
      781 gggtgtcgat ctcgtggatc tcggcattgc cctcgaccag ccgctccatc acccgccaca
      841 tccagccggt gccctcgcgg cacggagtgc actggccgca gctctcatgc ttgtagaaat
      901 aggagatgcg gctgatcgcc ttcaccacgt cggtcgactt gtccatgacg atgatcgcgg
      961 cggtgccgag gccggagccg aggtccttca gcccgtcgaa atccatcggc gcgtcgacga
     1021 tctgctcggc cggcaccagg ggcaccgagg agcctccggg gatgacggcg aggagattgt
     1081 cccagccgcc gcggattccg ccggcatgcc ggtcgatcag ctcgcggaac ggcaccgaca
     1141 tggcgtcctc gacgacgcac ggcttgttca cgtggccgga gatctggaag agcttggtgc
     1201 cctcgttctt ctcgcgcccg atgcccttga accagctcgc cccccggcgg aggatggtcg
     1261 ggacgacggc gatggattcg acattgttga cggtggtcgg gcagccgtag aggccggccc
     1321 ccgccgggaa gggcggcttg aggcgcggca tgcccttctt gccttccagg ctctcgagca
     1381 tcgccgtctc ttcgccgcag atgtaggcgc cggcgccgcg gtggacgaag aggtcgaaat
     1441 cgtagccgga tttggccgcg ttcttgccca gcagccccgc atcataggcc tgctggaccg
     1501 ccgcctcgag cgtctgcgcc tcgcggatga actcgccgcg aatgtagatg tagccggcgc
     1561 gggcgcgcat cgcgaagccg gcgatcaggg cgccttcgat caacttgtgc ggatcgtggc
     1621 ggatgatctc ccggtccttg caggagccgg gctcggattc gtcggcgttg atgaccagga
     1681 aattgggccg cccgggggtc ggtgccttgg gcatgaacga ccatttcatg cccgtgggga
     1741 agccggcgcc gccgcgcccg cgaagccccg aattcttgat ctcgtcgatg atcgcgtctt
     1801 cgccgatcgc catcagcgtc ttggtgtcgt cccagtcgcc gcgcgcctgc gcgcccttca
     1861 gcccccagtc ctggaagccg tagaggttgg tgaagatccg atccttgtcc tcgagcatca
     1921 ggagcccatt ccgaaataaa gcgccgccac cagggcgacg atgacgacga gcgccaccat
     1981 cttgatcatg cccttcaaga ccttgagcgc gatgaccaga gcgatgactc cgacgacgat
     2041 cgggaggagc tgttccattt cagccgccca ccttttccag cccgcgagtc gccagccagg
     2101 cccaggcccc ataggccaga aaccagacga tcaaagcggc gcccagcgca tagccgaagc
     2161 gccagcggcc gggatcctcg tcgatcctct gcgatgccag aagggcaagc cagccaagca
     2221 ggatcaaggt cgcggccgcg cagaaaaggc gaactcccca gatgcgcgcc cgcatccgcg
     2281 ccgggtcttg cgcggcgtag gccgaccccg ccttccctag cggaaggaag agcgtttgac
     2341 cggccttatc ctgtgcgaac gcgctggccg tgcagcccag gaggtcgaag cgaccgatcg
     2401 agatcatgcc ggccgctccc cgccccagcg tgagcgataa tcgtaattct cgccgaccat
     2461 ctccggaagc gacgtcactc cgccctccgg atcgctggtc cgccggttga cgccctgtgg
     2521 ccccggcctc ggagtctcgc cgcgggcgag cgcctcaagg atcgcggacg tcgtctcgaa
     2581 ggtgaggtcc tcgtaattgt cgtcgttgat ctggaccatc ggagcgttgg cgcaggcgcc
     2641 gaggcattcg acctcggaga gggtgaagag gccgtccgcg gtggtctgcc ccttcttcat
     2701 tcccttcgcc ttgcaggcgg cgaagacgtc gtcggagccg cgaagcatgc agggcgtggt
     2761 gccgcagacc tggacgtgga agcggccgac cgggcgcagg ttgaacatcg tgtagaaggt
     2821 cgcgacctca taggcccgga tgtagggcat gccgagatag gcggcgacat attcgatcac
     2881 cgggatgggc agccatccct gggtgtccgt ctccgccccc acctggcgct gggcgaggtc
     2941 gagcaagggg ataatcgcgc tctgctgccg cccgggcggg tagcggtcga cgagcagctt
     3001 cgccttcctg tcgttctcgc gcgtgaaggc gaacgcgccc cagcgggcgc ggacctcgtc
     3061 gagatcgatg gaggtgggga ggatatcagc catccgggcg tccatcgtcg tcccgggtcg
     3121 ggcgcttgcc gcggctggag ccggaggcga gcgcgaccag cagcagcagg accagggccg
     3181 ccgcgatgcc gccgaccgtc cttaccgcgg tgccgtcggc ccggtcgtcg ggggtgaaca
     3241 ggtagagcgc accaaccgcc acgacggcga cggtcagcgc gagcatggcg agggtgcgtc
     3301 tgtcagccat gggccgcggg cctccccttc ctgccgagca gccgccgcgc gagtgcgacc
     3361 gcctcgacga gaagcgccgc ggcgatgatg cccatcaggg cgagcggaag gacgagaaaa
     3421 gctcccccgc cgagggccgg acccatcagc acggcgtagg cgaggagcgc ggtgacagcc
     3481 gcggcgagga aatatcgccg cgccttcacc ggaagctgcg cgaacaccgt cgccggcgcg
     3541 agcacgaaca tcgcgagcag caggatttgg ctcaccggtc gatctccccg aagacgatgt
     3601 cgagcgagcc gaggatggcg gtgacgtcgg cgagcatgtg gccgcgggtc atgaagtcca
     3661 tcgcctggag gtgcgagaag ccggtggggc ggatcttgac ccggtagggt ttgttggtgc
     3721 cgtcggcaac gaggtagatt ccgaactcgc ccttcgggct ctcggtggcg acgtagacct
     3781 cgcccgcggg gacgtggaag ccctcggtgt acagcttgaa gtggtggatg agcgcttcca
     3841 tcgattgctt catctcggcc cgcttgggcg gaacgacctt gcggtccagc gaagcgatcg
     3901 gtccctgcgg catctgctcg aa
//
LOCUS       DASWNU010000398         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_66233, whole genome shotgun sequence.
ACCESSION   DASWNU010000398 DASWNU010000000
VERSION     DASWNU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66233"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /gene="rplD"
                     /locus_tag="VGB57_07945"
     CDS             complement(<1..206)
                     /gene="rplD"
                     /locus_tag="VGB57_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY0131322.1"
                     /translation="MASPKAATLGKTTKTDLPELFSEPFHQSLVHEAARADLAARRRG
                     TASTLTRGEISMTTAKAWRQKGTGR"
     gene            complement(199..828)
                     /gene="rplC"
                     /locus_tag="VGB57_07950"
     CDS             complement(199..828)
                     /gene="rplC"
                     /locus_tag="VGB57_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY0131323.1"
                     /translation="MAAILGKKLGMTQVFQEDGRVVPVTVIEAGPCKVTAVRDAERDG
                     YRAVQLAFGEVKQGKLTKAEEGHLKKAGAAPMRHLVEFRDEDLGAEEGPKVGDDVTVE
                     GFEAGQKVKVAGISIGKGFQGGIKRHNFSRGPVSHGSHNVRAPGSIGASAFPARVFKG
                     MRMPGQMGNKRVTQKGLEIADVDAERNLLLIKGSVPGGRNAILEIRTDG"
     gene            complement(837..1148)
                     /gene="rpsJ"
                     /locus_tag="VGB57_07955"
     CDS             complement(837..1148)
                     /gene="rpsJ"
                     /locus_tag="VGB57_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY0131324.1"
                     /translation="MAAQKIRIRLKAYDHDAIDRAAKEIVETAERTGASVSGPVPLPT
                     EKNVYCVIRSPFKDKDSREHFEIRTHKRLIDILQPSPKTVDSLQRLDSLPAGVDIEIK
                     L"
     gene            complement(1306..>1820)
                     /locus_tag="VGB57_07960"
     CDS             complement(1306..>1820)
                     /locus_tag="VGB57_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY0131325.1"
                     /translation="GTVVTGKVEQGIVHTGDEVEIVGIKDTTKTTVTGVEMFRKLLDE
                     GQAGDNIGALLRGTKREEIERGQVLCKPGSITPHTKFKAEVYCLKKEEGGRHTPFFSG
                     YRPQFYFRTTDVTGIANLPEGTEMVMPGDNVEMTIELIQPIAMDPGLRFAIREGGRTV
                     GSGVVAEVIE"
BASE COUNT          235 a          597 c          624 g          364 t
ORIGIN      
        1 cggcccgtgc ccttctggcg ccacgccttg gcggttgtca tcgagatctc gccgcgggtg
       61 agcgtcgagg ccgtgccgcg gcggcgcgca gccaggtcgg cgcgtgcggc ctcgtgcacc
      121 agggactggt ggaagggctc ggagaagagc tccggcagat cggtcttggt ggtcttgccg
      181 agtgtggcgg ccttggggct agccatcggt ccgaatctcc agaatcgcgt tgcggccgcc
      241 ggggacggag cccttgatca gcagcaggtt gcgctcggcg tcgacgtcgg cgatctccaa
      301 gcccttctgg gtcacccgct tgttgcccat ctggcccggc atccgcatgc ctttgaagac
      361 gcgggcgggg aaggcgctgg cgccgatcga ccccggggcg cgaacgttgt gggaaccgtg
      421 gctgacgggg ccgcggctga agttgtggcg cttgatcccg ccctggaagc ccttgccgat
      481 cgagattccg gcgaccttga ccttctgccc ggcctcgaag ccctcgacgg tgacgtcgtc
      541 gccgaccttc gggccctcct cggcgccgag atcctcgtcg cggaactcga ccaggtggcg
      601 catcggcgcc gcgccggcct tcttcaggtg gccttcctcg gccttggtca gcttgccctg
      661 cttgacctcg ccgaaagcga gctggacggc gcggtagccg tcgcgctcgg cgtcgcggac
      721 cgcggtcact ttgcaggggc ccgcttcgat cacggtcacc ggaacgaccc ggccgtcctc
      781 ctggaagacc tgggtcatgc cgagcttctt gccgaggatc gccgccatca ggactcctag
      841 agcttgatct cgatgtcgac gcccgccggc agcgagtcga ggcgctgcag cgagtcgacg
      901 gttttcggcg aaggctggag gatgtcgatc agccgcttgt gggtgcggat ctcgaagtgc
      961 tcgcgcgagt ccttgtcctt gaacggggag cggatcacgc agtagacgtt tttctcggtc
     1021 ggcagcggca ccgggcccga gacgctggcg ccggtgcgct cggcggtctc gacgatctct
     1081 ttggcagccc gatctatggc gtcgtggtcg tacgccttca gtcggatccg aattttctgt
     1141 gccgcgatgg ttgccacttc gatacgtctc ttgtctttct cttcgcttta ccgcgcaaag
     1201 agggcggggc cccttttggt ggggcgccgc cccggtcgta tgtccttgca gttgtctccc
     1261 cgcccgaagg tggggttggg gcgggtggag gcccgccccg ggtcgctact cgattacctc
     1321 ggcgacgacc cccgagccga cggtgcggcc accctcgcgg atggcgaacc gcaggcccgg
     1381 atccatggcg atcggctgga tcagctcgat cgtcatctcg acgttgtcac ccggcatcac
     1441 catctccgtc ccctcgggga ggttggcgat cccggtcacg tcggtggtcc ggaaatagaa
     1501 ctgaggccgg tacccggaga agaacggagt gtggcgacca ccctcctcct tcttcaggca
     1561 gtagacctct gctttgaatt tggtgtgcgg cgtgatcgag ccgggcttgc agagaacctg
     1621 gccgcgctcg atctcctcgc gcttggtgcc gcggaggagg gcgccgatgt tgtcgcctgc
     1681 ctgcccctcg tcgaggagct tgcggaacat ctcgaccccg gtgaccgtgg ttttggtcgt
     1741 gtccttgatg cccacgatct cgacctcgtc gccggtgtgc acgatgcctt gctcgacctt
     1801 gccggtcacg acggtgccgc
//
LOCUS       DASWNU010000399         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_169559, whole genome shotgun sequence.
ACCESSION   DASWNU010000399 DASWNU010000000
VERSION     DASWNU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169559"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..627)
                     /locus_tag="VGB57_07965"
     CDS             complement(109..627)
                     /locus_tag="VGB57_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131326.1"
                     /translation="MRKLFLIALALAASPAFAQPPPEPVARAMAEIEAHRVGMQVGCP
                     IPVPALWNAPAWEQRSAAARRAAFEDCLSGVMQRERDRLAGLQERIAVLRAEAPEADW
                     SAADVALDLKWAELDALTGKLRTRDKWADTAVRVLDTFTGPGAPFGPRPQPYNRCAPF
                     RCDSSVSFPGIR"
     gene            769..1734
                     /locus_tag="VGB57_07970"
     CDS             769..1734
                     /locus_tag="VGB57_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0131327.1"
                     /translation="MDLTDPLWANARRLIASGGKAEAILLVHRRAAGGDPSAWFVLAC
                     WKWEGAVLPRDLGQARALFARAGEAGHSGAAACITNLLANGVAGERDWPGALARLRSE
                     ARSDPQRQAASRLLGKMRLTPEGDPPAIRPSRPLSLAPEVHLFPGLFTPAECDHLARA
                     AAPNLEPSFVVDEETGRAELDPVRTSDGCELHWFVEDPAVHALNRRLAAATGTSVDQG
                     EPLQILRYSVGQQYRRHLDAVPGLANQRVLTAIVYLNQGYEGGGTCFPRVGLTVEGRK
                     GDALVFRNASADGRVDPLSEHAGLPVTSGTKFIASRWIHQLPIAP"
     gene            1963..2550
                     /gene="petA"
                     /locus_tag="VGB57_07975"
     CDS             1963..2550
                     /gene="petA"
                     /locus_tag="VGB57_07975"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY0131328.1"
                     /translation="MATVEHSDGTLAAGPDSPSDASLRRRDFINIAAVSVAGVGAAVT
                     LYPLVNQMNPSADVLALASIEVDVSAIQPGQAIKTIFRKQPLFIRHLTPPEIAAGMQM
                     PGDLRDPQSLADRTAEGKPQWLITMGVCTHLGCVPLGAAEGENKGSYGGYFCPCHGSH
                     YDTAGRIRRGPAPKNLEVPAYVFRSDTVVAVGEEA"
     gene            2553..>3199
                     /locus_tag="VGB57_07980"
     CDS             2553..>3199
                     /locus_tag="VGB57_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY0131329.1"
                     /translation="MSFPWAEEYRPKNGFTRWIDQRLPLPRLVYGAVGGGYPVPRNLN
                     YFWNFGVLAGIALVIQIVTGIILAMHYVPYDQAAFRSVEHIMRDVNQGWLLRYLHANG
                     ASFFFIVTYIHIFRGLYYGSYKAPRELVWMLGLVILLLMMATAFMGYVLPWGQMSFWA
                     SKVITGLFGAIPLIGEPVQNWLLGGYAPDQPTLNRFFSLHYLLPFVIAGVVILHI"
BASE COUNT          469 a         1124 c         1090 g          516 t
ORIGIN      
        1 cattgcgagg agcggagcga cgaagcaatc cagcttctgt gggcatcgcg ggcgcgggct
       61 ggattgcttc gctgcgcccg caatgacggg gtctcccgtc tccgccccct accttattcc
      121 ggggaacgag acgctcgaat cgcagcgaaa gggggcgcag cgattatagg gctggggcct
      181 ggggccgaag ggggcgccgg ggccggtgaa ggtgtcgagg acccgcaccg ccgtgtcggc
      241 ccatttgtcc cgggtcctga gcttgcccgt caaggcgtcg agctcggccc atttcaaatc
      301 cagcgcgacg tccgccgccg accagtcggc ctccggggcc tcggcgcgaa ggacggcgat
      361 gcgctcctgc agcccggcga gccggtcccg ctcgcgctgc atgacgcccg acaggcaatc
      421 ctcgaacgcc gcccgccggg ccgcggcgga gcgctgctcc caggccgggg cgttccagag
      481 ggcgggcacc gggatcgggc agccgacctg catgccgacc cggtgcgcct cgatctcggc
      541 catggcccgg gcgaccggct cgggcggcgg ctgggcgaaa gcgggggagg cggcaagggc
      601 gagggcgatg aggaagagct tgcgcatgag cggcctccgt ttcggtgccg aaccgatagg
      661 cccggggcgg cttaacggca cttgaatgcc ggggggcgcc ggatctttcc accgcgcgcc
      721 ggcgcaaagt cgctccggga tgagcggcgg gcgtgataga gccgggccat ggacctcacc
      781 gatccattgt gggcgaatgc tcggcggctg atcgcctcgg gcggaaaggc cgaggcgatc
      841 ctgctcgtcc accggcgagc ggcgggcggc gatccctccg cctggttcgt cctggcctgc
      901 tggaagtggg agggggccgt ccttccccgc gacctcgggc aggcccgcgc cctcttcgcc
      961 cgggcgggcg aggcggggca ctcaggcgcg gcggcgtgca tcaccaatct gctcgccaac
     1021 ggagtcgcgg gggagcgcga ctggcccgga gccctcgcca ggcttcggtc ggaggcccgc
     1081 tcggacccgc agcggcaggc cgcctcgagg ctgctcggga agatgcggct gaccccggag
     1141 ggcgacccgc cggcgatccg gccgtcgcgg ccattgagcc tcgcccccga agtccacctg
     1201 ttcccgggcc tttttacccc ggcggaatgc gatcatctcg cccgggccgc ggcgccgaac
     1261 ctggagccct ccttcgtcgt cgacgaggag acgggccgtg ccgagctcga cccggtccgg
     1321 acgtcggacg gttgcgagct gcactggttc gtcgaggatc ccgcggtcca cgcgctcaac
     1381 cgccgcctcg ccgcggcaac cggaacgtcc gtggaccagg gcgagccgct ccagatcctc
     1441 cgctactcgg tcggccagca atatcgccgc cacctcgatg ccgtgccggg gctggccaac
     1501 cagcgggttc tgaccgccat cgtctacctc aaccagggct atgaaggcgg cgggacatgc
     1561 tttccaaggg tcggcctgac ggtcgagggg cgcaagggcg acgccctcgt cttccgcaac
     1621 gcttcggcgg acggccgggt cgatcccctg tcggagcatg ccgggctgcc ggtgacgagc
     1681 ggcaccaagt tcatcgccag ccgctggatc caccagcttc cgatcgcgcc ctgaggaagg
     1741 gccgcgcgca gatcgggagc cgtgcggccc gcttttcctc tgccggccgg gtccgttgcg
     1801 ccgacaagcg ccggcggctt taaaatcgct gttggcaaag cgctcctcac gcgtctatca
     1861 ggcgcgccaa gccgctcagg ggaggcttcc tgctccctta ggcgcggcgc tgcgcgggct
     1921 cttcctcccc gtgcattcct gttcgaagcg ctgaaggaca ttatggcgac ggttgaacac
     1981 agcgacggga cgctggccgc gggcccggac tccccgtccg acgcgagcct gaggcggcgc
     2041 gacttcatca acatcgcggc ggtgagcgtc gccggagtcg gggcggcggt cacgctctac
     2101 ccgctagtca accagatgaa cccgtcggcc gacgtgctcg ctctggcgtc gatcgaggtc
     2161 gacgtgtcgg cgatccagcc cggccaggcg ataaagacca tcttccgcaa gcagccgctc
     2221 ttcatccggc atctgacccc gcccgagatc gcggcgggaa tgcagatgcc gggcgacctt
     2281 cgcgaccccc agtcgctcgc cgatcggacc gccgagggca agccgcaatg gctgatcacc
     2341 atgggcgtgt gcacccatct cggctgcgtc ccgctgggcg ccgccgaggg cgagaacaag
     2401 ggctcctacg gcggctattt ttgcccctgc catggttccc attacgacac cgccggccga
     2461 atccgcagag gcccggcccc caagaatctc gaagtgccgg cctatgtctt caggtccgac
     2521 acggtcgtcg ccgtcggcga ggaggcatag aaatgagctt cccctgggca gaggaatatc
     2581 gtcccaagaa cggcttcacg cgctggatcg accagcgcct gccgcttccg cgtctcgtct
     2641 acggggcggt gggcggcggc tatccggtgc cgcgcaacct caattacttc tggaatttcg
     2701 gagttctggc cgggatcgcc ctggtcatcc agatcgtcac cgggatcatc ctggcgatgc
     2761 attacgtgcc ctacgaccag gccgccttcc ggtcggtcga gcacatcatg cgcgacgtca
     2821 accagggctg gctgctgcgc tacctccacg ccaacggcgc cagcttcttc ttcatcgtca
     2881 cctacatcca catcttccgg ggcctctatt acggctccta caaggctccg cgcgagctgg
     2941 tgtggatgct cgggctcgtc atcctgctgc tgatgatggc gacggcgttc atgggctacg
     3001 tgctgccctg ggggcagatg agcttctggg cttcgaaggt gatcaccggc ctcttcggag
     3061 ccattccgct gatcggcgag ccggtccaga actggctgct cggcggctac gcgcccgacc
     3121 agccgaccct gaaccgcttc ttctcgctcc actacctctt gccgttcgtg atcgccggag
     3181 tggtcatcct ccacatctg
//
LOCUS       DASWNU010000400         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_404119, whole genome shotgun sequence.
ACCESSION   DASWNU010000400 DASWNU010000000
VERSION     DASWNU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404119"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VGB57_07985"
     CDS             <1..930
                     /locus_tag="VGB57_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0131330.1"
                     /translation="LIGAEAGDVAIVPSVSYGMATAMANVPVAASQKIVTLADDFPSG
                     ILASRALAQRAEARVVTVARPGTGDWSAAILEAIDRDTALVVAPHTHWVCGTLFDLEA
                     IGRRCRSVGAALVLDTTQSTGALPLDLAAVDPDYLVATSYKWLMGPYSLGFLYVAPRR
                     QQGRPLEEAWIVRRNARDFRSLTDYDEGFAAGARRFDMGERANFALLPVAGAAIEQLL
                     DWGVADVAETLGAKTAAIEARLLERDVTGQAGRAPHFLSVRFPGGLPDGIEERLAAAD
                     VHVSLRGERMRITPHLYNDEADAERLFSALWPN"
     gene            958..>1941
                     /locus_tag="VGB57_07990"
     CDS             958..>1941
                     /locus_tag="VGB57_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0131331.1"
                     /translation="MRLKSLLLACSLVAAPAGAAEFTDSIKADYDKRLAAMFVDFHRN
                     PELSFKENRTARILAKALRAEGATVTEGVGGTGVVAVMRNGDGPTVLIRADMDGLPVE
                     EKSGVSYPSKARQVGIDGLEMPVMHACGHDVHITSLVGTARQLAAAKDRWRGTIVFIA
                     QPAEERVGGAKAMLADGLYTRFPKPDYALGFHVNSGMRTGTVSGADSLMYSSADSVDV
                     TVHGVGAHGAAPHTGKDPVYIGAQIVTALQSIVSREVGPLSPAVITVGSFHAGTKHNI
                     ISDRADLQITVRANDEATRRQLLTAIDRIAKGIARAHGVAESRLPEVKVSEG"
BASE COUNT          296 a          681 c          684 g          280 t
ORIGIN      
        1 ctgatcgggg ccgaggccgg cgacgtcgcc atcgtcccct cggtcagcta cggcatggcg
       61 acggcgatgg ccaacgtccc cgtggcggcg agccagaaga tcgtcaccct cgccgacgac
      121 ttcccgtccg ggatcctcgc ctcgcgggcg ctggcgcagc gggcggaggc gcgggtcgtg
      181 accgtcgctc ggcccgggac gggcgactgg agcgccgcca tcctcgaagc gatcgaccgc
      241 gacaccgctc tcgtcgtcgc gccgcatacg cattgggtgt gcgggaccct gttcgacctc
      301 gaggcgatcg ggcggcgctg ccgctcggtc ggggcggccc tggtgctcga cacgacccag
      361 tcgaccggcg ccctgccgct cgacctcgcc gcggtcgacc ccgactatct ggtcgcgacg
      421 tcctacaaat ggctgatggg gccctacagc ctcggcttcc tctacgtcgc tccccgccgt
      481 cagcaggggc ggccgctcga ggaggcgtgg atcgtccgcc gcaacgcccg ggatttccgg
      541 agcctcaccg attacgacga aggcttcgcc gccggagccc ggcgcttcga catgggcgag
      601 cgggccaatt tcgcgctgct gccggtggcg ggggcggcga tcgagcagct cctcgactgg
      661 ggggtcgccg acgtcgcgga gacgctcggc gcgaagacag cggcgatcga ggcccggcta
      721 ctcgagcgcg acgtgaccgg ccaggcgggg agggcgccgc atttcctctc cgtgcgattc
      781 ccgggcggcc tgccggacgg gatcgaggag aggctcgcgg ccgcggacgt ccatgtcagc
      841 ctgcgcggcg agcggatgcg gatcacgccg catctctaca acgacgaagc cgatgcggaa
      901 cggctgttct cggcgctctg gccgaattag gatggtgcga attcccggag gtcatgaatg
      961 cgtctgaagt ccctgttgct tgcctgctcc ctcgtcgccg ctcccgccgg cgcggcggag
     1021 ttcaccgact cgatcaaggc cgattacgac aagcgcctcg ccgcgatgtt cgtcgacttc
     1081 catcgcaatc cggagctcag cttcaaggag aaccgcaccg cgcggatcct cgccaaggcg
     1141 cttcgagccg agggcgcgac ggtgacggaa ggggtcggcg gcaccggcgt ggtcgccgtc
     1201 atgcgcaacg gcgacgggcc caccgtattg atccgggcgg acatggacgg cctgcccgtc
     1261 gaggagaaga gcggggtctc ctacccgtcc aaggcgcggc aggtcgggat cgacggactc
     1321 gagatgccgg tgatgcacgc ctgcggacac gacgttcaca tcacctcgct ggtcggaacc
     1381 gcgcgccagc tggcggcggc gaaggacagg tggcggggca cgatcgtctt catcgcgcaa
     1441 ccggccgagg agcgggtcgg cggcgccaag gcgatgctcg ccgacggcct ctacacgcgc
     1501 ttccccaagc ccgattacgc gctcggcttc cacgtcaatt cgggaatgcg caccggcacg
     1561 gtgagcggcg cggactcgct catgtacagc tcggccgaca gcgtcgacgt caccgtccac
     1621 ggcgtgggtg cgcacggagc ggcgccgcat accggcaagg acccggtcta catcggcgcc
     1681 cagatcgtca ccgccttgca gtcgatcgtc agccgcgagg tggggccgct gtcgcccgcc
     1741 gtcatcaccg tcggctcctt ccatgccgga acgaagcaca acatcatctc cgaccgcgcc
     1801 gacctccaga tcacggtccg ggccaatgac gaagcgacgc ggcggcagct gctgacggcg
     1861 atcgaccgga tcgccaaggg catcgcccgg gcccatgggg tcgccgagag ccggcttccg
     1921 gaggtcaagg tgtcggaagg c
//
LOCUS       DASWNU010000401         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_352615, whole genome shotgun sequence.
ACCESSION   DASWNU010000401 DASWNU010000000
VERSION     DASWNU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352615"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3314
                     /locus_tag="VGB57_07995"
     CDS             <1..3314
                     /locus_tag="VGB57_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0131332.1"
                     /translation="MKAQRLEALPAPAATRSAARPPAGILAQRHSTLGGGSMSAPPIL
                     DAGRPLEPATRSEMERGFGRDFGAVRVHDDARAHDNARSLGAVAYAAGNDIVFGQGHY
                     APQTAAGRALIAHELAHSVQQGGVQMKADGSLPVAADQRLEAEADRAAAAVTAGRAAP
                     TLSRIGAPAVFRQTDANAVSTDAGAKPAQGGAEEEVPGVGKVVVESELPTGAGVGATY
                     ASVVMPPLSVPAKGTGSWIQANYSQAAQGGSLAYKANVTGTKFEVITDPEPSYGDIWV
                     QKYGFKTMQELGAAVKAAAAKKPLASANAMTIVNGLAAGSLTNAKCDVDHIVEKQLGG
                     NNIPNNLQLLSKSVNRSSGSRIRPVLADLANKVRTAIRPQLEQIQLRFPIVTVEAEPT
                     KKDDYYLASCEVETLLRSSEIVGDPGVKAGADGTPVGLTALGKSVIAPIRGKGETAIG
                     EPASLLITGMRLEKYVRGTKGGADKVAASLSPKGLLPGPKPVALEAKPGAAPGPVAPP
                     PGSTAAPLEAPAEKGPAPTEWRQLNLPKNAAKDVQAHYPSLSNVAMKTLEIKEDGLHG
                     TAILTPSVAKFLGPIEIRFSPTTLEAVKDIDAAALKTPAQAFFRFTKGSLALTLAPSF
                     VPTGQLDFEIGPQGKPLMLGFVKARYEAGAFIASGELTPAGKIPAVDKAAGTVEYHSQ
                     KGWSGKLTAESSSIPGSTVTAELGFRPGKAGLEAYGKGGFSTTVKGSPLDLTLDWDGT
                     TVSYGGKVTVKDPMPLVKEVTLEGNYRGDQLKLTGTAPIVWKSIKANMSVTYRRKDGE
                     EGKFSGAATVEFPKEGKVGGSLDLNFDEAGNFWGKGQVSYQLTPQLRPTLTAEYTKAR
                     RLKMSGEVAIGDIALTKEWPAPGGAKKPIIKGVGVKFSVPTPVPFVTAYGEIKGSLGV
                     GYGVGPVMLKGVVLSGELYPLEDDPKPVVTLKGKFSLPAYGELYGTFGAYIGAEILFG
                     VAGAKGGIEVSPSLRISGEAGLAFEAGYASGGFSFAAEAAITAALTAKLKVDFAAEIY
                     ALGGLASYRWTYPLAAVSRQIGPQFKMTLGKIAYAPDGTITWPSLSQIKFEPESIDPM
                     KLVSDLMKEGKETKQ"
     gene            3311..3967
                     /locus_tag="VGB57_08000"
     CDS             3311..3967
                     /locus_tag="VGB57_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131333.1"
                     /translation="MSGFPGSPKTLKGGFVEMDSEGKTVLRTVAFQYNPMTLSRTLAL
                     RAAKTDSGDRLEGLRLVGPPAETMKIDIELDATDRLERPDDNPETVANGIAPELADLE
                     MMVSPGSADLEAANRLALTGTIEVLPLPSPMLLLVFGANRTLPVRINDFSIVEEEFDV
                     NLNPIRAKVSLGLRLLSIDDLAYGSKGAELFMVALKRREQLSRRKPPAIQSLGLKRAP
                     "
BASE COUNT          736 a         1359 c         1398 g          562 t
ORIGIN      
        1 caatgaaggc gcagcggctc gaggcgctcc cggcgccggc ggcgacccgg tccgcggcgc
       61 ggccgccggc gggaatcctg gcgcagcgcc attccacgct cggcggcggc agcatgagcg
      121 cgccgccgat cctcgatgcg gggcggccgc tcgagccggc gacccgaagc gagatggagc
      181 gcggcttcgg ccgggatttc ggcgcggtgc gagtccacga cgacgcccgc gcccacgaca
      241 atgcccgctc gctcggcgcc gttgcctatg ccgcgggcaa cgacatcgtc ttcggccaag
      301 gccattacgc cccccagacc gcggccggcc gggcgctgat cgcccatgag ctcgcccata
      361 gcgtccagca gggcggcgtg cagatgaagg cggacggctc gcttccggtc gccgccgacc
      421 agcggctcga agcggaggcg gaccgggcgg cggcggccgt aaccgccggc cgcgccgcgc
      481 cgaccctgtc gcggatcggc gcgcctgcgg tgtttcgcca gaccgacgcc aacgcggtct
      541 cgaccgacgc cggcgccaag cccgcccaag gcggtgccga ggaggaggtg cccggcgtcg
      601 gcaaggtggt ggtcgagagc gagctgccga cgggcgcagg cgtcggggcg acctatgcga
      661 gtgtcgtgat gccgccgctc tcggtgcccg ccaagggcac gggatcatgg atccaggcca
      721 attacagcca ggcggcgcag ggcggctctc tcgcctacaa ggcgaacgtc acgggcacca
      781 agttcgaggt catcactgat ccggaaccga gctacggcga tatctgggtc cagaaatacg
      841 gcttcaagac gatgcaggag ctcggcgcgg cggtgaaggc ggccgcggcc aaaaagcccc
      901 tcgcctcggc caatgcgatg acgatcgtca acggcctggc cgccggcagc ctgaccaacg
      961 ccaagtgcga cgtcgatcac atcgtcgaga agcagctggg cggcaacaac atccccaaca
     1021 acctccagct gctgagcaag agcgtcaacc gctcctccgg ctcgcggata cggccggtgc
     1081 tcgccgacct cgcgaacaag gtgcgcaccg cgattcgacc gcagctcgag cagatccagc
     1141 tgcgcttccc gattgtcacc gtcgaggcgg agccgaccaa gaaggacgac tattatctgg
     1201 cgagctgcga agtcgagacg ctgctccggt cctccgagat cgtcggcgat ccgggcgtca
     1261 aggcgggggc cgacggcacg cccgtaggcc tgaccgcact cggaaaatcg gtcatcgcgc
     1321 cgatcagggg caagggagaa acggcgatcg gggagccggc aagcctgctg atcaccggaa
     1381 tgcggctcga gaaatatgtg cgcgggacca aaggcggagc cgacaaggtg gcggcctcac
     1441 tctcccccaa ggggcttctg ccggggccga agccggtcgc gctcgaggcg aagccagggg
     1501 cggcgcctgg gccggtggcg cctccgcccg gatcgacggc ggcgccgctt gaagcgccgg
     1561 cagaaaaggg cccggcgccg accgaatggc gccagctcaa cctgcccaag aacgcggcca
     1621 aggacgtgca ggcgcactat ccctcgctca gcaatgtcgc gatgaagacc ctcgagatca
     1681 aggaggacgg cctgcacggg accgccatcc tcacaccttc ggtggcgaag ttcctcggcc
     1741 cgatcgagat acggttcagc ccgacgacgc tcgaggcggt caaggacatc gacgcggccg
     1801 cgctcaagac gcccgctcag gccttcttcc gcttcactaa agggtcgctc gcgctgacgc
     1861 tggcgcccag cttcgtcccg accgggcagc ttgatttcga gatcggcccg caggggaagc
     1921 cgttgatgct cggtttcgtc aaggcaaggt acgaagcggg cgccttcatc gcctcgggcg
     1981 agctgacgcc cgcgggcaag attccggcgg tcgacaaggc ggcgggcaca gtcgaatatc
     2041 attcgcagaa gggctggtcg gggaaactga ccgcggagag ctcttccatt cccggcagca
     2101 ccgtcaccgc cgagctcggc ttccgtccgg gcaaggccgg gctcgaagcc tatggcaagg
     2161 gaggcttcag cacgacggta aagggcagcc cgctcgatct tacactcgac tgggacggca
     2221 ccacggtcag ctatggcggc aaggtgacgg tgaaggatcc gatgccgctg gtgaaggagg
     2281 tcacgctcga ggggaactat cggggcgacc agctgaagct gaccggcacg gcgccgatcg
     2341 tgtggaagtc gatcaaggcc aatatgagtg tcacctaccg gcgcaaggac ggcgaggagg
     2401 gcaaattctc gggcgccgcc acggtcgagt tcccgaagga gggcaaggtc ggcggctcgc
     2461 tcgacctcaa tttcgacgag gccggcaact tctggggcaa gggccaggtg agctatcagc
     2521 tgacgccgca gctgcgaccg accctgaccg ccgaatatac caaggcccgc cggctcaaga
     2581 tgtcgggcga ggtcgcgatc ggcgacatcg cgctgaccaa ggaatggccg gcgccggggg
     2641 gcgccaagaa gcccatcatc aagggcgtcg gcgtcaagtt cagcgtcccc acaccggtgc
     2701 cgttcgtcac cgcctacggg gagatcaaag gctcgctcgg ggtcggctac ggggtcgggc
     2761 cggtgatgct gaagggggtg gtgctaagcg gcgagctcta cccgctcgag gacgatccca
     2821 agcccgtggt aacgctgaag ggcaagttca gcctgcccgc ctacggggag ctgtacggca
     2881 ccttcggggc ctatatcggg gccgagatcc tgttcggcgt cgccggcgcc aagggcggga
     2941 tcgaggtgtc gccgagtttg cggatcagcg gcgaggccgg cctggccttc gaggcgggct
     3001 atgcctcggg cggcttcagc ttcgccgccg aggccgcgat caccgccgcc ctcacggcga
     3061 agctcaaggt cgatttcgcc gccgaaatat acgccttggg cggtctcgcc tcctatcgct
     3121 ggacctatcc gctggcggcg gtctcccggc agatcgggcc gcagttcaag atgaccctcg
     3181 gcaagatcgc ctacgcgccc gacggcacca tcacctggcc gagcctctcc cagatcaagt
     3241 tcgagcccga gagcatcgat ccgatgaagc tcgtctcgga cctgatgaag gaaggcaagg
     3301 aaaccaagca atgagcggct tcccaggctc tcccaaaacg ctcaagggcg ggttcgtcga
     3361 gatggactcc gagggcaaga cggtgctgcg gaccgtcgcc ttccaatata atccgatgac
     3421 cctgagccgg acgctggcgc tgcgcgccgc gaagaccgat agcggcgacc ggctcgaagg
     3481 actgcggctg gtcggcccgc ccgccgagac gatgaagatc gacatcgagc tcgacgccac
     3541 cgaccgcctg gagcgtccgg acgacaatcc cgagaccgtc gccaacggca ttgcgccgga
     3601 gctggccgac ctcgagatga tggtcagtcc gggcagcgcc gacctcgagg cggccaaccg
     3661 gctcgccctc accggcacga tcgaggtgct gcccctgccc tcaccgatgc tgctcctcgt
     3721 cttcggagcg aaccggaccc ttccggtgag gatcaacgac ttctcgatcg tggaggagga
     3781 gttcgacgtg aacctcaacc cgatccgggc gaaggtctcg ctcgggctga ggctgctgtc
     3841 gatcgatgac ctcgcctacg gcagcaaggg cgcggagctg ttcatggtcg cgctcaagcg
     3901 gcgcgagcaa ttgtcccgcc gcaagccgcc cgcgatccag tcgcttggcc tgaagagggc
     3961 gccatgagcg acgatttcgc cttcaacagc cgttaccgcg gggtcaggct gcgcacccgg
     4021 acggccgccg acgggacggt cgagacctat gtcgg
//
LOCUS       DASWNU010000402         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_378111, whole genome shotgun sequence.
ACCESSION   DASWNU010000402 DASWNU010000000
VERSION     DASWNU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378111"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2162
                     /locus_tag="VGB57_08005"
     CDS             <1..2162
                     /locus_tag="VGB57_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0131334.1"
                     /translation="AVTITGAGWTAALRGGRLGTLLAGTAIGAGIGTMHYVGMAAVKL
                     PGRFHWDETMVAASLLVGIALSTAALAEHRRRPRLLPWRPALLFTLAICGLHFTAMAA
                     ASVHPDSRLAVPAAAMDSTVLTLAVVAMALVILAIGFMMVLFDRKLARNAIEEARRLR
                     TFADAAVEGLVVIDGERIVDANRSFLGLAGFETLGDAPERLSDLLIEVNLAGLSMAPD
                     APPVECRLLRADGESREVELLLRPLAWRGADLRILAVRDISERKEAAERIAHLAFHDS
                     LTGLPNRSVFADHLARTVAKADESADHVAVMCVDLDGFKAVNDIYGHPAGDALLIAAA
                     QRLRSAVRGHELVARLGGDEFAVVQSGGDQPGHAGLLAQRIVEALCEPFALGQDTVRV
                     SASVGVALFPADAADPESLVKNADMALYRAKADGRGTARFYEAAMDEALRRRRQLEAD
                     LGQAIGRGELDIHYQPLADLESGSILGFEALLRWNHAELGAIGPATFIPLAEESGLIL
                     KLGAWVLREACAEAARWTPALRLSVNLSPVQFTQGDLAAEVEAILAGTGLDPARLELE
                     VTEGLLIKDADRAIVILERLKALGVQISMDDFGTGYSSLSYFRMFPFDKVKIDQSFIQ
                     DMIGNPQARAIIRSVIGLGQGLGMPVVAEGVETEEQLEALRAEGCDQVQGYWISRPGP
                     IANFDHVVIERNSSAARKPAEAAAGGERRLNRGRSRR"
     gene            complement(2163..3023)
                     /locus_tag="VGB57_08010"
     CDS             complement(2163..3023)
                     /locus_tag="VGB57_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0131335.1"
                     /translation="MTEDPKPPFPDQPQPMPGLTQRMEPVPDHGEESYRGSRRLEGKK
                     AIVTGGDSGIGRAVAIAFAREGADLLISYLPQEEEDAQAVRALVEAAGRTCILFPGDI
                     ASADHCRALVARAAEAFGRIDILVNNAAHQDTFSDIEQIDEKEWDYTFAVNIHAMFYL
                     TKAAVAHMKPGSSIINTTSVQADDPSQELLAYASTKGAIQNFTGGLAQMLAERGIRAN
                     CVAPGPIWTPLIPATMPSEKVQSFGEQVPMKRPGQPRELAPVYVMLASDEASYISGAT
                     IAVTGGKPII"
BASE COUNT          455 a         1134 c         1086 g          522 t
ORIGIN      
        1 cggcggtcac gatcaccggc gccggctgga ccgccgccct gcgcggcggc cgcctcggca
       61 ccctcctcgc cgggacggcg atcggggccg gcatcggcac catgcattat gtcggaatgg
      121 cggcggtgaa gctgcccggg cgcttccact gggacgagac gatggtcgcg gcttcgctgc
      181 tcgtcgggat cgctctcagc accgccgctc tggccgagca ccggcgcagg ccccggctgc
      241 ttccgtggcg gccggccctg ctgttcaccc tcgccatctg cggccttcac ttcacggcca
      301 tggcggcggc ctccgtccat cccgattcgc ggctcgcggt gccggcggcg gcgatggatt
      361 cgaccgtcct gaccctcgcc gtggtggcga tggctctggt catcctcgcc atcggattca
      421 tgatggtgct gttcgaccgc aagctcgcca ggaacgcgat cgaggaagcg cggcggcttc
      481 gcaccttcgc cgacgccgcg gtcgaagggc tggtcgtgat cgatggcgag cggatcgtcg
      541 acgccaatcg cagcttcctc ggactggccg gcttcgaaac cctcggcgac gcgcccgagc
      601 ggttgtcgga cctgctcatc gaggtcaatc tcgccggcct ctccatggcc ccggacgctc
      661 cgccggtcga atgccgactg ctccgcgccg acggcgagag ccgggaagtc gagctgcttc
      721 ttcgcccgct cgcctggcgc ggcgccgatc ttcgaatcct cgccgtgcgc gacatttccg
      781 agcgcaagga ggcggccgag cggatcgccc atctcgcctt ccacgattcc ctgaccgggc
      841 tgcccaaccg ttccgtcttc gccgaccatc tcgcccgcac cgtcgccaag gcggacgaga
      901 gcgcagatca cgtcgccgtg atgtgcgtcg atctcgacgg cttcaaggcg gtcaacgaca
      961 tctacggcca cccggccggc gacgccctcc tgatcgcggc ggcgcagcgg cttcgctcgg
     1021 cggtgcgcgg ccacgagctg gtcgcgcgcc tcggcggcga cgaattcgcg gtggtccagt
     1081 cgggcggcga ccagcccggc catgccggcc tcctcgccca gcggatcgtc gaggctctct
     1141 gcgaaccgtt cgcgctgggc caggacacgg tccgcgtcag cgccagcgtc ggagtcgccc
     1201 tcttccccgc cgacgccgcc gaccccgaaa gcctggtcaa gaatgccgac atggcgctct
     1261 accgcgccaa ggccgacggc cgcggcaccg cccgcttcta cgaggcggcg atggacgagg
     1321 cgctgcgccg gcgccgccag ctcgaagcgg atctgggcca ggcgatcggc cgcggcgagc
     1381 tcgatatcca ctatcagccg ctcgccgatc tcgagagcgg ctcgatcctc ggcttcgagg
     1441 cgctgctgcg ctggaatcat gccgagctgg gagcgatcgg cccggccacc ttcatcccgc
     1501 tcgccgagga gagcggcctg atcctcaagc tcggcgcatg ggtgctgcgc gaagcctgcg
     1561 ccgaggcggc gcgctggact ccggccttga ggctctcggt caacctgtcg cccgtccagt
     1621 tcacccaggg cgacctcgcc gccgaggtcg aggcgatcct cgccggaacc ggcctggacc
     1681 cggcgcggct cgagctcgag gtcaccgagg ggctgctgat caaggacgcc gaccgggcga
     1741 tcgtcatcct cgagcggctg aaggcgctgg gcgtgcagat ctcgatggac gatttcggaa
     1801 ccggttactc gtcgctcagc tatttccgga tgttcccgtt cgacaaggtc aagatcgacc
     1861 agagcttcat ccaggacatg atcggcaacc cccaggcccg cgccatcatc cgctcggtga
     1921 tcggcctcgg ccaggggctc ggaatgcccg tcgtcgccga gggggtggag accgaagagc
     1981 agctcgaagc gctgcgcgcc gaaggctgcg accaggtgca gggctattgg atcagccgcc
     2041 cggggccgat cgccaatttc gaccacgtcg tgatcgagcg caactcgagc gcggcgcgga
     2101 agccggcaga ggcggctgcc gggggcgagc gacgcctcaa ccgcggtcgg tcgcggcggt
     2161 gatcagatga tcggcttgcc gccggtcacc gcgatcgtcg ctccggagat gtagctggcc
     2221 tcgtcgctcg ccagcatcac gtagacgggc gcaagctcgc gcggctgccc cggccgcttc
     2281 atcggcacct gctccccgaa gctctgcacc ttctcgctcg gcatcgtcgc cgggatgagc
     2341 ggagtccaga tggggcccgg agccacgcaa ttggcgcgga tcccgcgctc cgcaagcatc
     2401 tgcgccaggc cgccggtgaa gttctggatc gcgcccttgg tcgatgcata agcgagcagc
     2461 tcctggctcg gatcgtcggc ctggacggac gtcgtgttga tgatcgacga gcccggtttc
     2521 atgtgggcca ccgccgcctt ggtcaggtag aacatggcgt ggatgttgac cgcgaaggta
     2581 tagtcccact ccttctcgtc tatctgttcg atgtcggaga aggtgtcctg gtgcgccgcg
     2641 ttgttgacga ggatgtcgat ccgcccgaac gcctccgccg cccgagccac cagcgcccgg
     2701 caatggtcgg cgctcgctat gtcgccgggg aagaggatgc aggtgcgccc cgccgcctcg
     2761 accagcgccc gaacggcctg ggcgtcttcc tcctcctgcg gcaggtagga gatgagcagg
     2821 tccgccccct cgcgcgcgaa ggcgatcgcg acggcgcgcc cgatgccgct gtccccgccg
     2881 gtgacgatcg ccttcttgcc ctcgagccgc ctggagccgc gatagctctc ctcgccatgg
     2941 tccggcaccg gctccatccg ttgggtgagg cccggcatcg gctgaggctg gtccgggaag
     3001 ggaggcttgg gatcttcggt cacgtcgggc tccttgcagg ctggagcacc gctaaccgga
     3061 agcggacgat tgggttcccg cctctcaggt gaacgcgaca taggtgtcag caccgatttt
     3121 cgatgaacga tcatcggccg gcatgagcca agatccccct ctcccccgtg gggagaggta
     3181 gggtgagggg gttcggc
//
LOCUS       DASWNU010000403         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_404155, whole genome shotgun sequence.
ACCESSION   DASWNU010000403 DASWNU010000000
VERSION     DASWNU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404155"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VGB57_08015"
     CDS             <1..396
                     /locus_tag="VGB57_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HEY0131336.1"
                     /translation="GSLQTFADENDRRRLTPAAVKAVLRLVKAWDAGNAEGAALLGVS
                     ESTWDRMKSGRWDGVLSQDQLTRASALIGVFKGLHLLFADGMADRWPRLPNRGPIFDR
                     LSPIQAMIEGGIPRMLETRQYIDALRGGL"
     gene            383..1090
                     /locus_tag="VGB57_08020"
     CDS             383..1090
                     /locus_tag="VGB57_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY0131337.1"
                     /translation="MAASDAGDMDGLPLVREAFPRTVRLVASARLRAAVLEPLADGED
                     GLALLAEIEGATSGRLIAEERGSGGFSAEELVHGVPHAKFINASFAYAKPREPMRFSP
                     ADRGAWYAALAVETCIAEVGYHLTNALADANDFAAVVDYCEMLASMAGVFVDLRQAPD
                     HASLDPDRASGYPAGNAVAAAARAAGHNGIIYPSARQAGATCLAALWPNVVQSVVQGA
                     MYRLTWSGSPDFQVEPL"
     gene            complement(1218..>2081)
                     /locus_tag="VGB57_08025"
     CDS             complement(1218..>2081)
                     /locus_tag="VGB57_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0131338.1"
                     /translation="GVDEARERLREGVELPLKNPEAFRRLGIRPAKGFLLYGPPGTGK
                     TLLAKATAREAEANFIATKSSDLLSKWYGESEQQIARLFARARQVAPTVIFIDELDSL
                     VPARGGGLGEPQVTERVVNTILAEMDGLEDLGSVVVIGATNRPNLIDPALLRPGRFDE
                     LIYVAVPNEAGRRKILSIHTAKMPLAEDVDLDSFAARTDRFTGADLEDLVRRAGLFAL
                     RRSIDAVQVTGSDFEMALDETRASVTPEMEKDYEKIQESLKRDAMSSGAGGIGFISPG
                     MLTPRGPKGTE"
BASE COUNT          328 a          671 c          721 g          361 t
ORIGIN      
        1 gggtcgcttc agaccttcgc cgatgagaat gatcgccggc ggctgacccc ggcggctgtg
       61 aaggccgtcc tcaggctcgt taaggcctgg gatgccggaa acgccgaggg tgccgctttg
      121 cttggcgtgt ccgagagcac ctgggatcgc atgaaaagtg gccggtggga cggcgtgctg
      181 tcgcaggatc aactgactcg cgcctccgct ctgatcggcg tgttcaaggg gctgcacttg
      241 ctgttcgcgg acgggatggc ggatcgctgg ccgcggcttc ccaatagagg tccgattttc
      301 gaccgcctgt cgccgatcca ggcaatgatc gaagggggca ttccgcgaat gctcgagacc
      361 cgacaatata tagacgcgct gcgtggcggc ctctgacgcc ggcgatatgg acggcctccc
      421 gctcgtccga gaggcgtttc cacgaacggt gcggctggtc gccagcgcga gattgcgcgc
      481 cgccgtgctc gagccgctcg ccgacggcga ggacggactc gcattgctcg ccgaaatcga
      541 gggggcgacc agcggccggc tcatcgccga ggaaaggggc agcggagggt tcagcgcgga
      601 ggaactggtt cacggcgttc cccacgccaa gttcatcaat gcgagcttcg cttatgccaa
      661 gccgcgcgag ccaatgcgct tcagtccggc cgatcgcggc gcctggtatg ccgcgctcgc
      721 cgtcgaaacc tgcatcgccg aagtcggtta ccatctcacg aacgccctgg cggatgcgaa
      781 cgacttcgcg gcggtcgtcg actattgcga aatgctggcg agcatggccg gcgtcttcgt
      841 cgatctccgc caggccccgg accatgcctc tctcgatccc gatcgggcgt caggttatcc
      901 cgctggcaat gcggtcgccg ccgccgcgcg cgccgccggc cacaacggca tcatctatcc
      961 atcggcccga caagccgggg cgacgtgcct cgcggcgctt tggccgaatg tcgttcagtc
     1021 ggtggtccaa ggcgcaatgt atcggctgac ctggtccgga agcccggact ttcaggtcga
     1081 gccgctttga agacgtgccg gaaagtgccg gcatccgtca ccctgagctt gcttcagggc
     1141 caggtctcca tccgcccagc ggtctccgga ctggatcctg aaacaagttc aggatgacga
     1201 ggtggcgccg ccagccccta ttccgtcccc ttcggccccc gcggagtcag catgccgggc
     1261 gagatgaagc ctatgccgcc ggcgcccgag ctcatcgcgt cgcgcttcag gctttcctgg
     1321 atcttctcgt aatccttttc catctccggg gtgacggagg cgcgggtctc gtccagcgcc
     1381 atctcgaaat ccgagccggt gacctggacc gcatcgatcg agcggcgaag ggcgaagagg
     1441 ccggcccggc ggacgaggtc ctcgaggtcg gctccggtga agcggtcggt ccgggcggcg
     1501 aagctgtcga ggtcgacgtc ttcggcgagc ggcatcttcg cggtgtggat ggagaggatc
     1561 ttgcgccggc cggcctcgtt cgggacggcg acgtagatga gctcgtcgaa ccggccgggc
     1621 ctgagcaagg ccggatcgat caaattcggg cggttggtgg cgccgatgac gaccaccgag
     1681 ccgaggtcct cgagcccgtc catctcggcg aggatggtgt tgaccacccg ctcggtcacc
     1741 tgcggctcgc cgagaccgcc gccgcgggcg gggacgaggc tgtccagttc gtcgatgaag
     1801 atgacggtcg gggcgacctg gcgggcgcgg gcgaagaggc gggcgatctg ctgctcgctc
     1861 tcgccatacc atttggagag gaggtcggag gatttggtgg cgatgaagtt cgcctccgcc
     1921 tcgcgcgccg tagccttggc gagcagggtc ttgcccgtgc caggagggcc gtaaagcaag
     1981 aagcccttcg ccggccggat gccaagccgc cggaatgcct cggggttctt gagcggaagc
     2041 tcgacacctt cgcgaagccg ctcacgcgcc tcgtcgacgc c
//
LOCUS       DASWNU010000404         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_93711, whole genome shotgun sequence.
ACCESSION   DASWNU010000404 DASWNU010000000
VERSION     DASWNU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93711"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2240)
                     /locus_tag="VGB57_08030"
     CDS             complement(<1..>2240)
                     /locus_tag="VGB57_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0131339.1"
                     /translation="DWISDYSDGDDQLFGEDGDDQLWIDRTSAGLATNALLDGGIGND
                     RLGFRGFSVDDGGDRSHSSSRVTMNGGEGADYFYLSDANVAVIDGGPGDDHVAIDLFG
                     DYTVTLGAGRDLLELADPRNFFFTGRPVVVTDFVTGAGGDRLDLIDYVADLLFAWDPA
                     TNPFGTYLQIAQQGADVVLQIDRAGAFDADGFLTVLTFQNVAMDAFTAENLGGWASDG
                     SAVAGVTFPVTTGDDFIIGLSGNDVVVGSDGADRIRGGAGNDRLEGGEGLDIIVGEFG
                     DDEIIGGGGNDQLLDKHSGNDTLRGGAGNDLVYVQRESGAVSRVLLDGGDGDDYVSYI
                     AMVGSSAETILLGGGGNDNIVLNGDQGATIDAGPGDDGVLIYLSNGSFDMLLGPGADT
                     IEIGFDSQRDGIASFTVRDFQAGAGGDLFRLDPTFDGYWSNWDGVTSLFKTGHLQLVQ
                     QGADTLLQIDRDGGGDAFMDFVTFRSATVGGVSQNMRGSNLAPLLAPPTNGDDTIEGT
                     PGDDAIDGLNGADTIYGRAGNDRLSGGDGNDRLFGEEDNDSLSGGAGDDFLVGGIGHD
                     SLDGGAGADRAEGGAGDDTYFVDSAADVAFELPSEGTDLLVTRVSYVLGANIELMQAA
                     AIGGVEPLSLTGNALGNTIWATFGSNSIDGGAGADVMYGYGGNDVYFVDNAGDAAVEF
                     EGHGTDLVATSVNYVLGANIENMQARSIPGTDPLSLTGNALGNIIWGNFGDNSLDGGT
                     GADVMY"
BASE COUNT          378 a          749 c          744 g          369 t
ORIGIN      
        1 gtacatgacg tcggcgccgg tgccgccgtc gagcgaattg tcaccgaaat tgccccagat
       61 gatattgccg agcgcattgc cggtcagcga cagcgggtcc gtccccggga tggagcgcgc
      121 ctgcatgttc tcgatgttgg cgccgaggac gtagttgacg ctggtcgcga ccaggtcggt
      181 gccatggcct tcgaactcga ccgccgcatc gccggcattg tcgacgaaat agacgtcgtt
      241 gccgccatag ccgtacatca cgtccgcgcc ggcgccgccg tcaatcgaat tgcttccgaa
      301 agtcgcccag atcgtgttgc caagcgcatt gccggtgagc gacagcggct cgacgccccc
      361 gatggccgca gcctgcatca attcgatatt cgcgccgagg acgtagctca cacgggtgac
      421 gaggaggtcc gtgccttccg agggaagctc gaaggcgacg tccgccgccg aatcgacaaa
      481 ataagtgtcg tcgcccgcac cgccctcggc gcggtcggcg ccggcgccgc cgtcgaggga
      541 gtcgtggccg atgcccccga ccaggaagtc gtccccggcc ccgcctgaaa gcgaatcatt
      601 gtcctcttcg ccgaacaggc ggtcgttgcc gtcgccgccc gaaagacggt cgttgcccgc
      661 acggccgtag atggtgtcgg cgccgttgag gccgtcgatg gcgtcgtctc cgggcgtgcc
      721 ttcgatcgtg tcgtcgccgt tcgtcggcgg cgcgagcagg ggcgcgaggt tcgaccccct
      781 catgttctgg gagacgcctc cgaccgtggc gctgcgaaag gtcacgaaat ccatgaaagc
      841 gtctcccccg ccgtcgcggt cgatctggag caaagtatcg gcgccctgct ggaccagctg
      901 caaatggccg gtcttgaaga ggctggtgac gccgtcccag ttcgaccaat agccgtcgaa
      961 agttggatca agcctgaaca ggtcgcctcc cgcccccgcc tgaaagtcgc gcacggtgaa
     1021 cgaggcgatg ccgtcgcgct gcgaatcgaa gccgatctcg atcgtatccg cgcccgggcc
     1081 gagcagcatg tcgaagctgc cgttgctgag atagatgagc acgccgtcgt cgccggggcc
     1141 ggcgtcgatc gtggcaccct gatcgccgtt gaggacgatg ttgtcgttgc cgccccctcc
     1201 gagcaggatc gtctcggccg acgagccgac catggcgata tagctcacat agtcgtcacc
     1261 gtcgccacca tcgaggagga cgcggctgac cgcgcccgac tcgcgctgca cgtagaccag
     1321 atcgttgccg gcgccgccgc gcagcgtgtc gttgcccgaa tgcttgtcca acagctggtc
     1381 gttgccaccg ccgccgatga tctcgtcgtc gccaaactcg ccgacgatga tgtccaggcc
     1441 ctcgccgccc tcgagccggt cgttgccggc gccgccgcga atccggtcgg cgccgtccga
     1501 gccgacgacg acgtcgttgc ccgacaggcc gataatgaaa tcgtcgccgg tggtgaccgg
     1561 gaaggtcacg cccgccaccg ctgagccgtc gctcgcccaa ccgcccagat tctcggcggt
     1621 gaacgcatcc atggcgacgt tctggaaggt gagcacggtg aggaagccgt ccgcgtcgaa
     1681 ggcgcccgcg cggtcgatct gcagcacgac gtcggcgccc tgctgcgcaa tctgcaaata
     1741 ggtcccgaac gggttggtgg cggggtccca ggcgaacagg aggtcggcga cataatcgat
     1801 gaggtcgagc cggtcgccgc cggcgcccgt cacgaaatcg gtgacgacga cgggccgccc
     1861 ggtgaagaag aaattgcggg gatcggcgag ctcgagcaga tcgcggccgg cgccgagcgt
     1921 gacggtgtag tcgccgaaca ggtcgatggc gacgtgatcg tcgccggggc cgccgtcgat
     1981 gacggcgaca ttggcgtcgc tgagatagaa ataatcggcg ccctcgccgc cgttcatcgt
     2041 cacccggctc gaggaatggc tgcggtcgcc gccgtcgtcc acgctgaagc cgcggaagcc
     2101 gagccggtcg ttaccgatac cgccgtcgag gagcgcgttc gtggccaggc cggcactggt
     2161 ccgatcgatc cagagctggt cgtcgccgtc ctctccgaac agctgatcgt cgccgtcgga
     2221 atagtccgaa atccaatcgt
//
LOCUS       DASWNU010000405         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_483454, whole genome shotgun sequence.
ACCESSION   DASWNU010000405 DASWNU010000000
VERSION     DASWNU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483454"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1222
                     /locus_tag="VGB57_08035"
     CDS             278..1222
                     /locus_tag="VGB57_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131340.1"
                     /translation="MSFSFSDFSSSLEAGGAISPRDVLAIRRAVWPDGVVARAEADAL
                     FELHRLARLTSLEWCDFLIEAIADTVLNATEPRGYVDDDSAAWLIGQLGRDGQTIGAV
                     ELELVVKIVEKALNAPSSLKAWALAQIERCVLTGEGATRRDGDVRPGVVDEAEVDLLR
                     RLVLAPGGDGALVVSQSEAEMLWRIKDVTLGAANASGWLKLFVQAVGNHLMAYSSYRP
                     LERSEAARLETFMNDRRSSVLGFLSRMRRPDFDGAAKLLDGEESAEEHGSAVDAAAAV
                     TPTERAWLQFNVARDGKRDECEEALMRFLEEESGQTLP"
     gene            1368..>2632
                     /gene="rimO"
                     /locus_tag="VGB57_08040"
     CDS             1368..>2632
                     /gene="rimO"
                     /locus_tag="VGB57_08040"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0131341.1"
                     /translation="MATILPSPPKVGMVSLGCPKNLVDSERILSKLRSDGYQMSPDYA
                     GADVVLVNTCGFLDSAKEESLQAIGEAIAENGRVIVTGCMGKEAEVIRERFPEVLAVT
                     GAHQYEQVVGAVHVAAPMPPSAYLNLVPDGGLKLTPRHYSYLKISEGCNHRCSFCIIP
                     SLRGDLVSRRPDAILREAEKLVAAGTKELLVISQDTSAYGVDTRHASWPWKGGEVRAH
                     MTDLARELGRLGAWVRLHYVYPYPHVDRVIPLMAEGLVLPYLDIPFQHAAPSVLKAMK
                     RPANEAKVLERIRGWREICPDIAIRSTFVVGFPGETDSDFEYLMQWLEEAQLDRVGAF
                     RFEPVQGAAANSLPGAVPEEVKEERYARLMELTARISAARLQAKVGREIDVLIDLADG
                     EGGATGRSKADAPEIDGEVHLRDAGALKQG"
BASE COUNT          440 a          920 c          859 g          413 t
ORIGIN      
        1 gacgacaggt ccatcgccag gctgacgaag ccgagcgcga cgaccgagcg cgggagtgac
       61 gggcgggagg ccatgggggt cactatggcc gaccttcctt accgtcatgc tgaacttgtt
      121 cagcatccag ccttccaccg ccccggcggg cgacagttga cctgaaagga gttcaggttg
      181 acgagaaagc cggcactccg gcactcaccc ccttagcccg gacttaaccg ttccccttta
      241 tggcccgctg gcgaaaccac agccagcggg tgggaccatg agcttctcct tttccgattt
      301 ctcctcgtca ctcgaggcgg gcggcgcgat cagcccccgc gacgtgctgg cgatccgccg
      361 cgccgtctgg ccggacgggg tcgtcgcacg ggcggaggcc gatgccctct tcgagctcca
      421 ccggctggcc cgcctgacga gcctcgaatg gtgcgacttc ctcatcgagg cgatcgccga
      481 cacagttctc aacgccaccg aaccgcgcgg ctatgtcgac gacgactcgg cggcatggct
      541 gatcggccag ctcggccgcg acgggcaaac gatcggggcg gtcgagctcg agctcgtcgt
      601 caaaatcgtc gagaaggcgc tcaacgctcc ctcctcgctc aaggcctggg cgctcgccca
      661 gatagagcgt tgcgtcctca ccggcgaagg cgcgacgagg cgggacggcg acgtccggcc
      721 cggcgtcgtc gacgaagccg aggtggatct gctgcgccgc ctcgtcctcg cacccggcgg
      781 cgatggagcg ctcgtcgtca gccagagcga agccgagatg ctgtggcgga tcaaggacgt
      841 gaccctcggc gccgccaacg cttcgggctg gctaaagctg ttcgtccagg cggtcggcaa
      901 ccatctgatg gcttattcct cctaccgtcc gctcgagcgc tccgaagcgg cgcgcctcga
      961 aaccttcatg aacgaccgcc gaagcagcgt cctcggcttc ctctcccgaa tgcgccgccc
     1021 ggacttcgac ggcgcggcga agctcctcga tggggaggaa agcgccgaag agcatgggtc
     1081 cgccgtcgac gcggcggcgg cagtgacccc caccgaacgc gcctggctcc agttcaacgt
     1141 cgctcgggac ggcaaacggg acgaatgcga ggaggccctc atgcgcttcc tggaagaaga
     1201 aagcgggcag acgcttccct gaggccttcc gcgcattgtc cgtcgccccg gcgaaagccg
     1261 ggcctcagga caagaaggca cagcgggtct ccccgaggtc ccggctttcg ccgggatgac
     1321 gggaccctag gaaaatccgg ccggggccac tatctagcgc ccttcccatg gccaccatcc
     1381 tcccctcccc gcccaaggtc ggcatggttt cgctcggctg cccgaagaat ctcgtcgatt
     1441 cggagcgcat cctgtcgaag ctgcggtccg acggctacca gatgtcgccc gattatgccg
     1501 gcgccgacgt cgttctggtc aacacctgcg ggttcctcga cagcgccaag gaagagagcc
     1561 tgcaggcgat cggggaagcg attgccgaga acggccgggt catcgtcacc ggctgcatgg
     1621 gcaaggaggc ggaggtcatc cgggagcgct tccccgaggt gctcgccgtc accggcgccc
     1681 atcaatatga gcaggtcgtc ggcgcggtcc acgtcgccgc tccgatgccc ccttcggcct
     1741 atctgaactt ggtgccggat ggcgggctca agctcacgcc gcgccattat tcctatctca
     1801 agatctcgga aggctgcaat caccgctgca gcttctgcat catcccgagc cttcgcggcg
     1861 acctcgtcag ccgccgcccc gacgccatcc tccgcgaggc ggagaagctg gtggcggccg
     1921 ggacgaagga attgctggtc atcagccagg acacgtcggc ctacggcgtc gacacccggc
     1981 acgcctcctg gccctggaaa ggcggcgagg tccgcgccca tatgaccgat ctcgcccgcg
     2041 agctcggtcg cctcggggcc tgggtgaggc tccattacgt ctacccctac ccccacgtcg
     2101 accgggtgat cccgctgatg gccgaggggc tcgtcctccc ttatctcgac atcccgttcc
     2161 agcatgcggc gccgtcggtg ctgaaggcga tgaagcggcc ggccaatgag gcgaaggtgc
     2221 tcgagcggat ccgcggctgg cgggaaatct gccccgacat cgccatccgc tcgaccttcg
     2281 tcgtcggctt ccccggcgag accgactcgg atttcgaata tctgatgcag tggctggagg
     2341 aagcccagct cgaccgcgtc ggcgccttcc gcttcgagcc ggtccagggg gcggccgcca
     2401 acagccttcc cggcgcggtt ccggaggagg tcaaggaaga gcgctacgca aggctgatgg
     2461 agctgaccgc ccggatctcc gccgccaggc tgcaggccaa ggtcggccgc gagatcgacg
     2521 tcctcatcga tctcgccgac ggcgaaggcg gcgccaccgg ccgctccaag gctgacgcgc
     2581 cggagatcga cggcgaggtt cacctgcgcg acgcgggcgc gttgaagcag gg
//
LOCUS       DASWNU010000406         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_197982, whole genome shotgun sequence.
ACCESSION   DASWNU010000406 DASWNU010000000
VERSION     DASWNU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197982"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1435
                     /locus_tag="VGB57_08045"
     CDS             185..1435
                     /locus_tag="VGB57_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY0131342.1"
                     /translation="MILSRYERMVARRYLLPGKGEGFIFLVAGISLVAVALGVAALIV
                     VMSVMNGFRAELFDKIVGLNGHAVVQGYGGQLPDWRDIVREARATPGVTDALPLIEQP
                     LMVSHEGRVEGVLVRGMERSDILRNETLNGKVLAGSLEALRPGGGNVAIGARLAEALG
                     ANVGGQISLISPEGRTTPFGTVPRIVSYNVSAIFEVGVYDYDKSFVIMPIEDAQTLLL
                     MGDAVGMVEIQTADADRVGEILEPLRASVSGRAGVADWRSMNTALFEALQVERVAMFV
                     ILSIIILVAVFNILSSLIMLVRAKTRDIAILRTMGATRRGLMKVFVTVGVIIGAIGIL
                     AGLGLGFLLLYFRQPFVNFVQFLTGQNLWDPSIRFLTELPSKTDPFEVLAIVTMALVL
                     SFLATLYPAWKAASTDPVQVLRYE"
     gene            1428..2099
                     /locus_tag="VGB57_08050"
     CDS             1428..2099
                     /locus_tag="VGB57_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0131343.1"
                     /translation="MNEVLAVKGLRRSFRQGGVTIDVLRGIDLAVRPGEIVALLGPSG
                     SGKSTLLQAVGLLEGGFEGSIRIAGEEVAELGDGNRTRVRRDTLGFVYQFHHLLPDFT
                     ALENVVIPQLVKGASREQAEERSKSLLGTLGLAERVTHRPSKLSGGEQQRVAVARALA
                     NRPALVLADEPTGNLDEATADIVFAEFLRLVRDEGSAALVATHNERIAAKMDRVLRLH
                     EGVLE"
     gene            2198..3136
                     /locus_tag="VGB57_08055"
     CDS             2198..3136
                     /locus_tag="VGB57_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131344.1"
                     /translation="MRNLILLIAGTSALLGGCGWMKKKDPPAEKSKTELAGEQRERIR
                     KACASDATYARLKELVFDEAARIRNSDPRNLDPLAAAAVVRMEEPLVKSRDDELNVTV
                     CSGRFILELPPGAENAFDGKRRLDAEVEYAAQAAVDGSGLVYQMDGAEPIIYRLATFG
                     LNGSTTRLEAGGPKRLPPPPAGAEPVLMPPVAETAPSPEPRPAPVPPVRTAENSPAPA
                     PAARVTVRPSFNCRHGKTRTEKMICSSPRLAAQDRAMASLYYDLMAGADPATRSHLRR
                     SRDSFLGRRERCGSESCVNSAYASRMAELRNIGRGD"
     gene            3210..>3395
                     /locus_tag="VGB57_08060"
     CDS             3210..>3395
                     /locus_tag="VGB57_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY0131345.1"
                     /translation="MAGPYVPLRIFSSYTMLEGSIEPKAIAKQAKRLDFPAAALTDRN
                     GLYAAMAFTDACREAGVQ"
BASE COUNT          548 a         1155 c         1147 g          545 t
ORIGIN      
        1 gccggtgatc atggcgatct tgtcgacgag tctcataacc ttactccgtc attgcgagga
       61 gcgaagcgac gaagcaatcc agcgggcgga ggcgcagcac tggattgctt cgcttcgctc
      121 gcaatgacgg ccagatgagc cgggaccccg gccttcgccg gggtggcggc gtgggggagc
      181 cgtcatgatc ctctcgcgct acgaacggat ggtcgcccgg cgctatctgc tgcccggcaa
      241 gggggagggg ttcatcttcc tcgtcgccgg gatcagcctc gtcgccgtcg cactcggagt
      301 cgccgccctc atcgtggtga tgagcgtgat gaacggcttt cgggccgagc tgttcgacaa
      361 gatcgtaggc ctgaacggcc atgcggtggt tcagggctat ggcggtcagc ttcccgactg
      421 gcgcgacatc gtccgcgagg cgcgggcgac cccgggcgtg accgacgcgc tgccgctcat
      481 cgagcagccc ctgatggtga gccacgaagg ccgggtcgag ggcgtgctcg tccgcggcat
      541 ggagcggtcc gacatcctgc gcaacgagac cctcaacggc aaggtcctcg ccggctcgct
      601 cgaggcgctc cgcccggggg gggggaatgt cgcgatcggc gcccgcctcg ccgaggcgct
      661 cggcgccaat gtcggcggcc agatcagcct gatcagcccg gagggtcgaa cgactccgtt
      721 cgggacggtc ccgcgaatcg tctcctacaa cgtctcggcg atcttcgagg tcggggtcta
      781 cgattacgac aaatcgttcg tgatcatgcc gatcgaggat gcccagaccc ttctcctgat
      841 gggcgatgcg gtcggaatgg tcgaaatcca gaccgccgac gccgaccggg tcggcgagat
      901 actcgagcct ctgcgcgcca gcgtcagcgg gcgggccggg gtcgcggact ggcgctcgat
      961 gaacaccgcc cttttcgagg cgcttcaggt cgagagggtc gcgatgttcg tcatcctctc
     1021 gatcatcatc ctcgtcgcgg tgttcaacat cctttcgtcg ctgatcatgc tggtgagggc
     1081 caagacccgc gacatcgcga tccttcgcac catgggcgcc acccgccgcg gcctgatgaa
     1141 ggtgttcgtg acggtagggg tgatcatcgg agcgatcgga atcctcgccg ggctcggcct
     1201 cggcttcctg ctcctctatt tccgccagcc cttcgtgaac ttcgtccagt tcctcaccgg
     1261 ccagaatctg tgggacccgt cgatccgctt cctgaccgaa ttgccctcca agaccgatcc
     1321 gttcgaggtc ctcgccatcg tcaccatggc cctggtgctg agcttcctcg ccacgctcta
     1381 ccccgcctgg aaggcggcga gcacggatcc ggtgcaggtg ctccgctatg aatgaggtcc
     1441 tggcggtcaa agggctcagg cggagcttcc ggcagggcgg ggtgacgatc gacgtcctgc
     1501 gcggcatcga cctcgcggtg aggccgggcg agatcgtcgc gttgctcggg ccgtccggct
     1561 cgggcaagtc gaccctgctc caggcggtcg ggctgctcga gggcggcttc gaaggctcga
     1621 tccggatcgc cggcgaggaa gtggcggagc tgggcgacgg caaccgcacc cgggtgcgcc
     1681 gcgacacatt gggcttcgtc taccagttcc accacctgct ccccgatttc accgcgctcg
     1741 agaacgtcgt catcccgcag ctcgtcaaag gggcgagccg cgagcaggcg gaagagcggt
     1801 cgaaatcgct gctcggaact ctcggcctgg ccgagcgggt cacccaccgg ccgagcaaat
     1861 tgtcgggcgg cgagcagcag cgcgtcgccg tcgcccgggc cctggccaac cgccccgccc
     1921 tggtcctcgc cgacgagccc accggcaatc tcgacgaggc gaccgccgac atcgtcttcg
     1981 ccgagttcct ccgcctcgtc cgggacgaag gcagcgccgc cctcgtcgcc acccacaacg
     2041 agcgcatcgc ggcgaagatg gatcgggtgc tgaggcttca cgagggcgtg ctggagtaag
     2101 gggcaccccc ggtcgccata ttggatcgta ggggaaagat gccccccgtg ctgcgttggc
     2161 ggcgggtgag tggggcagct ggagcgattc gatggcgttg cgtaatctga ttctgctgat
     2221 cgcgggaacc tccgctttgc tcggcgggtg cggctggatg aagaagaagg acccgcccgc
     2281 ggagaagagc aagaccgagc tggccggcga gcagagggag cggatccgca aggcctgcgc
     2341 ctccgacgcg acctacgccc gactcaagga gctggtgttc gacgaggccg cccggatccg
     2401 caattccgac ccccgcaacc tcgacccgct cgccgccgcg gcggtggtgc gaatggagga
     2461 gccgctggtg aagagccgcg acgacgagct caacgtcacc gtctgcagcg ggcgattcat
     2521 cctcgagctc cctcccggcg cggaaaatgc gttcgacggc aagcgccgcc tcgatgccga
     2581 ggtcgaatat gcggcccagg cggcggtcga tggaagcggc ctcgtctacc agatggatgg
     2641 cgccgagccg atcatctacc gcctcgccac cttcggcctc aacggatcga cgaccaggct
     2701 cgaggcgggg ggcccgaagc gcctcccgcc gcccccggct ggggcggaac cggtgctcat
     2761 gccgcccgtc gccgaaaccg cgccgagccc cgaaccgcgc ccggcgccgg ttcccccggt
     2821 acgtacggcg gagaattcgc ccgcaccggc gcccgcggcg cgggtcaccg tacgtcccag
     2881 cttcaactgc cgccacggca agacccgaac cgagaagatg atctgctcgt cgccgcggct
     2941 cgccgcccag gaccgggcga tggcctcgct ttattacgac ctcatggccg gcgcggatcc
     3001 cgccaccaga agccatttgc gccgaagccg cgactccttc ctcggccgcc gcgagaggtg
     3061 cgggagcgag agctgcgtca actcggccta cgcttcgcga atggcggagc tgaggaacat
     3121 cggtcgcggg gactgacggg ggcgaatagg tgtggaaaac ccggcgctcg gcgcttccat
     3181 cgccgcccgg cagccatcat aacgagggga tggccggccc ctatgttccg cttcgaatct
     3241 tctccagcta caccatgctg gaaggctcga tcgagcccaa ggccattgcc aagcaggcga
     3301 aaaggctcga ctttccagcc gcggcgctga ccgaccggaa cggcctctac gctgcgatgg
     3361 cgttcaccga cgcctgccgg gaagcgggtg ttcag
//
LOCUS       DASWNU010000407         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_404323, whole genome shotgun sequence.
ACCESSION   DASWNU010000407 DASWNU010000000
VERSION     DASWNU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404323"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VGB57_08065"
     CDS             <1..1146
                     /locus_tag="VGB57_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0131346.1"
                     /translation="DGFADVPGARVVDLRTSTVMPGFIDMHVHMRGLDDRLQARLQAA
                     QRDYEDEAFTALINARKTLLAGFTTVRDLGNEPRMITTLRDVIEAGQFAGPTIVAAGR
                     GISVSGGHGDVNGLNRDLTEIGLARADNTCNGPDDCRRAVRRQISAGADVIKFAATGG
                     VLSNVAGGLNQQMMADEMRAIVETAKTFGRKVAAHAHGVDGINAAIEAGVDSIEHGTF
                     TDDRTFALYRRTGAYYVPTLVAPAAAVADGERGALTPAQFDKAKQASGNAERSFARAV
                     RERVNIAFGTDSGVAPHGLNAREFGLMVKNGMAPAAAIHSATVAAATLLGRADRIGTI
                     EAGKDADIVAVRGDPTADIRLLENVQFVMKHGRVHKLGGERQLTTAD"
     gene            complement(1217..1645)
                     /locus_tag="VGB57_08070"
     CDS             complement(1217..1645)
                     /locus_tag="VGB57_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131347.1"
                     /translation="MIRAKEHSMALDATTAIIGLAGVGIGAAATYLAAKRNNETNLAI
                     AREKLQTENKLELEASKAIRELLLLPEWQLRSFEAIRKHIRGFSDDELRQLLIASGAV
                     SFDERGTDRELWGLRERNSDRLSPSSRLPRLSVKGPSPCR"
     gene            complement(1704..>2455)
                     /locus_tag="VGB57_08075"
     CDS             complement(1704..>2455)
                     /locus_tag="VGB57_08075"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0131348.1"
                     /translation="LMAAVLAKGETVIENAAREPEITDLAKCLIAMGADIEGLRTDTL
                     TIRGKDRLHGATYAVMADRIEAGSYACAAGITGGSLELVGARKETMPSILSGLSDAGL
                     IVEDTTDGVRVSADGGLKPLSISTAPYPAFPTDMQAQFMAMLTLAEGTSLLTETIFEN
                     RYMHVPELNRMGAEIEVRGRSAVVRGVEGLIGAEVMATDLRASMSLVLAGLAAKGETQ
                     VHRVYHLDRGYERLEEKLQAVGADVERVSDG"
BASE COUNT          401 a          840 c          799 g          415 t
ORIGIN      
        1 gacggctttg ccgacgtgcc gggcgcccgc gtggtcgatc tgcgcacgtc caccgtcatg
       61 ccgggcttca tcgacatgca cgtccacatg cggggactag acgaccggct gcaggcgcgg
      121 ctgcaggcgg cgcagcgcga ctatgaggac gaggccttca ccgccctcat caacgctcgc
      181 aagaccctcc tcgccgggtt caccaccgtg cgcgacctcg gcaacgagcc gcggatgatc
      241 accaccctgc gcgacgtcat cgaggccggt cagttcgcag ggccgaccat cgtcgcggcg
      301 gggcgcggca tctccgtctc gggcggccat ggcgacgtca acgggctcaa ccgcgacctg
      361 accgaaatcg gactcgcccg cgccgacaac acctgcaacg gccccgacga ctgccgacgg
      421 gcagtgcgcc ggcagatctc cgccggagcg gacgtcatca agttcgccgc gaccggaggc
      481 gtgctgagca acgtcgccgg aggcctcaac cagcagatga tggcggacga gatgcgcgcc
      541 atcgtcgaga ccgcgaagac cttcggccgc aaggtcgcgg cccatgccca cggggtcgac
      601 gggatcaacg cggcgatcga ggcgggggtg gattcgatcg agcatggcac cttcaccgac
      661 gacaggacct tcgccctcta ccgccgaacc ggcgcctatt acgtgccgac cctggtcgct
      721 cccgccgccg ccgtcgcgga cggcgagcgg ggcgctctga ctccggccca gttcgacaag
      781 gcgaagcaag cctccggcaa cgccgagcgc agcttcgccc gggccgtgcg cgagcgggtc
      841 aacatcgcct tcggcaccga cagcggagtc gctccccacg gcctcaacgc cagggagttc
      901 gggctgatgg tgaagaacgg aatggccccc gccgcggcca tccattcggc caccgtcgcc
      961 gccgcgaccc tgctcggccg ggccgaccgg atcgggacga tcgaggcggg caaggacgcc
     1021 gacatcgtcg cggtgcgcgg cgatcccacc gccgacatcc ggctgctcga gaacgtgcag
     1081 ttcgtcatga agcacggccg ggtccacaag ctcggcggcg agcgccagct gaccacggcc
     1141 gactgatcct tctgtaggcg gcttcggtca gcggcgatcg ccgacgagcg ggtcattcag
     1201 gtttccgttg ccactactac cggcacggag aaggcccttt gacggaaagg cgtggtaaac
     1261 gcgacgaggg agacaggcga tcggagttcc tttctcgtag tccccaaagt tctctatcgg
     1321 tcccccgttc gtcaaatgac accgctcctg acgcgatcag aagttggcgc agttcgtcgt
     1381 cactaaaccc ccgaatatgc tttctgatgg cttcaaagct tctgagttgc cactcgggta
     1441 gaagcaacaa ttctcttatg gctttggaag cttctagctc aagcttgttc tccgtttgaa
     1501 gcttctcgcg cgcgatggcc agattggttt cgttgtttcg cttggcagcg aggtaggttg
     1561 cggcggctcc gatcccgacg cccgccagtc cgataatcgc cgtcgtcgca tccagagcca
     1621 tggaatgttc ctttgctctg atcacctacc atttcttgcg tttgaaatga agtcgactgg
     1681 caactcctct tgccggtacg cacctagccg tcgctgacgc gctcgacatc ggcgccgacc
     1741 gcctggagct tctcctcgag ccgctcgtag ccgcggtcga gatggtagac ccggtggacc
     1801 tgggtctcgc ccttcgcggc gaggccggcg aggacgaggc tcatcgaggc cctgaggtcg
     1861 gtcgccatca cctcggcccc gatcagcccc tcgaccccgc ggaccaccgc cgagcggccg
     1921 cgaacctcga tctcggcccc catccggttg agctcgggga cgtgcatgta gcggttctcg
     1981 aagatcgtct cggtcagcag gctggtgcct tccgcgaggg tcagcatggc catgaactgg
     2041 gcctgcatgt cggtcgggaa ggcgggatag ggcgcggtcg agatggaaag cggcttcaat
     2101 ccgccgtcgg cgctgacccg gactccgtcg gtcgtgtcct cgacgatcag cccggcatcg
     2161 ctgaggccgg agaggatgga gggcatcgtc tccttgcggg cgccgaccag ctcgagcgag
     2221 cccccggtga tcccggccgc gcaggcatag cttcccgcct cgatccggtc ggccatcacg
     2281 gcataggtgg cgccgtggag ccggtccttg ccgcggatgg tgagggtgtc ggtcctcagc
     2341 ccctctatgt cggcgcccat ggcgatcagg catttggcga ggtcggtgat ctcgggttcg
     2401 cgggccgcat tctcgatcac cgtctcgccc ttggcgagca cggcggccat caggg
//
LOCUS       DASWNU010000408         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_248987, whole genome shotgun sequence.
ACCESSION   DASWNU010000408 DASWNU010000000
VERSION     DASWNU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248987"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /gene="rfbC"
                     /locus_tag="VGB57_08080"
     CDS             <1..593
                     /gene="rfbC"
                     /locus_tag="VGB57_08080"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY0131349.1"
                     /translation="SSTIIRALSTGRPEAMEIVPTALAGVFELRLDVRADSRGRFKRH
                     YCEKTFAKAGLMTRVVQVNHSITLGRGSLRGLHFQRPPHMEDKLVSCTFGRVFDVAVD
                     LRRGSPTYLRWTGVELDESTAFHIPKGCAHGFQALSEEAHLVYLHSEYYAAEAEGGVR
                     YDDPAIGIDWPLEPTNLSERDRAFAPILEEFESLTV"
     gene            590..1819
                     /locus_tag="VGB57_08085"
     CDS             590..1819
                     /locus_tag="VGB57_08085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0131350.1"
                     /translation="MKCRHCGNGSFIPFADLQNCPPSNSMLTRESLAEPEVYYPLVVE
                     VCDVCFLAQVDEHKKAEDIFSAEYTYFSSFSSSWLAHCRRFAGEAIERFGLGPESKVI
                     EVASNDGYLLQYFKEAGVQVLGIEPTANTAAVAQAKGIPTIVDFFGERFARERLAREG
                     QGDLVCGNNVFAHVPDINDFTAGLKAALAPGGVVSLEFPHLLKLIEESQFDTIYHEHF
                     SYLSLAATRSIFEAQGLKLFDVQRLPTHGGSLRVLGAHSDDESKPVSAAVEALLLEER
                     EAGLAGREVYERFQARIEAIRADFLGFLLEQRRAGKKVAGYGAAAKGNTLLNYCGVKG
                     NELIGFVADLSPHKQGKFLPGSHIPVLSPEAIDERRPDFIVIFPWNLKAEVIGQLGHA
                     REWGAKFVVAVPELAVI"
     gene            1819..2691
                     /locus_tag="VGB57_08090"
     CDS             1819..2691
                     /locus_tag="VGB57_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0131351.1"
                     /translation="MARVLLTGARGFIGGAVLRRLRADGHEAILLSSSGGSEDGEWRH
                     LDLLQASETEIARLVEKERITHCVHAAWYTNHSDYLVSDVNRGWAQASLRLEAGFRAG
                     GGERFVGLGTCLEYDRQGTAGRLSELSSPLGPETLYARCKSSVFEGLSERGGDFAWAR
                     VFFVYGPGDRPGRLIPLIVEGLAKGETVAARFGGLRRDYVHVDDLAGQLSRMTFAPVQ
                     GAVNTGTGAAARVADIFILAGEIAGRPDLVEVNDLVPAGEAESIEADMERFRSEIGDP
                     GTRPLRQGLAELMA"
BASE COUNT          447 a          939 c          925 g          480 t
ORIGIN      
        1 ccagctcgac gattatacgc gcgctctcga ccgggcggcc tgaggccatg gagatcgttc
       61 ccaccgccct cgccggcgtc ttcgagctgc ggctggacgt ccgcgccgac agccgcgggc
      121 gtttcaagcg ccattattgc gaaaagacct tcgcgaaagc cgggctgatg acccgagttg
      181 tccaggtgaa ccactcgatc acgctgggtc gcggctcgct tcgcgggctg cacttccagc
      241 ggcctcccca catggaggac aagctggtga gctgcacgtt cggccgggtc ttcgacgttg
      301 cggtcgatct gcgccgcggc tcgcccacct acctgcgctg gaccggagtg gagctggacg
      361 aaagcacggc cttccatatc cccaaggggt gcgcccacgg cttccaggcg ctcagcgagg
      421 aagcgcatct agtctacctg cacagcgaat attacgccgc cgaagcggaa ggcggagtcc
      481 ggtacgacga tcccgcgatc ggcatcgact ggccgctgga gcccaccaac ctttccgagc
      541 gggatcgcgc cttcgctccg atcctcgaag agttcgaaag cctcaccgta tgaaatgccg
      601 gcattgcggc aacggaagct tcattccgtt cgccgatctc cagaattgtc ccccttcgaa
      661 ctcgatgctg acccgcgaga gcctggccga gcccgaagtc tattacccgc tcgtggtcga
      721 agtctgcgac gtctgcttcc ttgcccaggt ggacgagcat aagaaagccg aggacatatt
      781 cagcgccgaa tatacctatt tctcctcttt ctcctcctcg tggctcgccc attgccggcg
      841 cttcgccggc gaggcgatcg agcgcttcgg gctcggtccc gagtcgaagg tgatcgaggt
      901 cgcgtccaac gacggctacc tgctccaata tttcaaggaa gccggagtgc aggtgctcgg
      961 catcgagccg acggccaaca ccgccgcggt cgcgcaggcg aagggtatcc ccaccatcgt
     1021 cgacttcttc ggcgagcgct tcgcgcgcga gcggcttgcg cgggagggcc agggcgacct
     1081 cgtctgcggc aacaacgtct tcgcccacgt tcccgacatc aacgacttca cggccgggct
     1141 caaggcggcg ctcgccccgg gaggagttgt ctcgctggag tttccgcacc tcctcaagct
     1201 catcgaggaa tcccagttcg acaccatcta ccacgagcat ttctcctatt tgtcgctcgc
     1261 cgcgacccgc tcgatcttcg aggcccaggg actcaagctg ttcgacgttc agcggctccc
     1321 cacccatggc ggctcgctgc gagtcctcgg cgctcactcc gacgacgagt cgaagccggt
     1381 ctcggccgcg gtggaggcgt tgctcctgga ggagcgcgag gcgggacttg ccggccgcga
     1441 ggtctacgag cgcttccagg cgaggatcga ggcgatccgg gccgacttcc tcggcttcct
     1501 cctcgagcag cgccgcgccg gcaaaaaggt ggcgggatac ggggctgcgg ccaagggcaa
     1561 taccctgctc aactattgcg gcgtcaaagg caacgaactg atcgggttcg tcgccgacct
     1621 gtcgccgcac aagcagggca agttccttcc gggaagccac attccggtgc tttcacccga
     1681 ggccatcgac gagcggaggc cggacttcat cgtcatcttc ccgtggaatc tgaaggcgga
     1741 ggtgatcggg cagctcggcc atgcgcgcga gtggggcgcg aagttcgtcg tcgcggtgcc
     1801 cgaactcgcg gtgatctgat ggcgcgggtc cttctcaccg gggcgcgcgg gttcatcggc
     1861 ggcgcggtcc tgcgccggct gcgggcggac ggtcatgaag cgatcctgct gtcgagctca
     1921 ggcggaagcg aggacggcga gtggcggcat ctcgacctgc tccaggcaag cgagaccgag
     1981 atcgcacggc tggtcgagaa ggagcggatc acccactgcg tccatgcggc ctggtacacc
     2041 aatcattccg attatctcgt ctccgacgtc aaccgcggct gggcgcaggc gagcctcagg
     2101 ctggaagcgg gctttcgggc cggaggcggc gagcgcttcg tcggcctggg cacctgcctc
     2161 gaatatgacc ggcagggcac ggccggccgc ctttccgagc tctcgtctcc gctcgggccg
     2221 gagacgcttt acgcccgctg caagagctcg gtgttcgaag gcctttcgga gcgcggcggc
     2281 gacttcgcct gggcgcgggt gttcttcgtc tacgggcccg gcgaccgccc gggccggctg
     2341 atcccgctca tcgtcgaagg cctggccaaa ggggagacgg tcgcggcgag gttcggaggc
     2401 ctgcgccgcg actatgtcca cgtcgacgac ctcgccggcc aattgtcgcg gatgacgttc
     2461 gcgccggtcc agggggcggt caacaccggc acgggcgccg ccgccagggt ggccgacatc
     2521 ttcatcctcg ccggcgagat cgccggacgg ccggacctgg tggaggtaaa cgatctcgtc
     2581 ccggccggcg aggcggagtc gatcgaggcg gacatggaac gcttccgttc cgagatcggc
     2641 gacccgggca cccggccgct tcggcagggc ctggcggagc tgatggcatg aggccccttg
     2701 tcgacgcccg gatcggctat gggggctaca gccgcgattt cagccgcccc ggggaccggc
     2761 gccgcttctc cgcttatgcg cgccagcgtg g
//
LOCUS       DASWNU010000409         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_352872, whole genome shotgun sequence.
ACCESSION   DASWNU010000409 DASWNU010000000
VERSION     DASWNU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352872"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VGB57_08095"
     CDS             <1..1334
                     /locus_tag="VGB57_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEY0131352.1"
                     /translation="KHTLRQPCAVEPFPGARFGFLAANVVGPDGATLFPGYAIRSFGK
                     GRRQVKVAIIGMTLKETPTVVTPAGVAGLAFRDEADTVNALIPKLKAQGADAIVVVVH
                     QGVYSKLGYNDQSCGAIEGELRPILDRLDPAVDVVASGHTHHAYVCDDGRINPARPFL
                     LTSAASYGRLVTDINLTIDPAAGRVTSKRAENIVVQSEAFTGSRGETPLTDVVPRYAA
                     EPAVAALVDRYAKAAEPIAARAVGRLAGPALKTNNEHGESVLGNLIADAQLAATRGAA
                     NGNARIALMNPGGIRADLVPAADGGISFGQLFATQPFANMMVVKALTGRQLRAVLEQQ
                     FASGSNTAANPYILSPSHNLRFAFDLSKPEGQRIVLLTVDGQPVRDEAVYRVAANNFL
                     AGGGDNFTVFREGTDSTGGVQDLDALEAYIAAAGNLAPPALGRVENRTPRR"
     gene            complement(1432..2886)
                     /locus_tag="VGB57_08100"
     CDS             complement(1432..2886)
                     /locus_tag="VGB57_08100"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY0131353.1"
                     /translation="MSDLESIPAIIDRLCEMFDESVAALRGALARYLLRGERPDAEAR
                     ARGLFAYPELRIDYSFDQPPSFPPRAFGRLNQPGRYAISIARPRHFRKYLVEQLEYLV
                     RDYKVDISVGLSASEIPYPYVLDGTDDLRLDGAHAADLSRWFPATELSHIGDEVADGT
                     WDYSRHADRPLALFDGPRVDFSLARLRHYTGTPVEHTQRYLLFTNYVRYVDEFVRWAV
                     EELKREDSPYEALSAPGGVLVTRDTADPVAEVAAGNWRKVQMPAYHLIAANGGGITLV
                     NIGVGPSNAKTICDHLAVLRPEVWLMIGHCGGLRPSQTIGDYVLAHAYLRDDHVLDGT
                     LPVEIPIPPIAEIQQALYDAAAEVTGEEPQELKRRLRTGTVVTTDDRNWELRIQSSAL
                     RFNQSRAVAIDMESATVAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANAFYERAIS
                     QHLRIGIETLRILKNQGGNLHSRKLRSFDEPPFR"
BASE COUNT          482 a          991 c         1104 g          483 t
ORIGIN      
        1 agaagcacac gctgcgccag ccctgtgcgg tcgagccctt ccccggcgcc cgcttcggct
       61 tcctcgccgc caacgtcgtc gggcccgacg gtgccaccct cttccccggc tatgcgatcc
      121 gcagcttcgg caagggccgc cgccaggtga aggtcgcgat catcggaatg acgctgaagg
      181 aaaccccgac ggtggtgact cccgcgggag tcgcgggcct cgccttccgc gacgaagcgg
      241 acacggtgaa cgccctgatc ccgaagctca aggcgcaagg cgccgacgcg atcgtcgtcg
      301 tggtccacca gggcgtctat tccaagctcg gctataacga ccagagctgc ggggcgatcg
      361 agggcgagct gcgtccgatc ctcgaccggc tcgacccggc ggtggatgtg gtcgcctccg
      421 gccacaccca ccatgcctat gtctgcgacg atggccggat caatcccgcc aggccgttcc
      481 tgctgaccag cgcggcgagc tacggccggc tcgtcaccga catcaacctc accatcgatc
      541 ccgccgccgg cagggtgacg tcgaagcggg ccgagaacat cgtcgtccag agcgaggcct
      601 tcaccggctc gcgcggcgag actccgctga ccgacgtcgt gccgcgctac gccgccgagc
      661 ccgcggtggc ggccttggtc gaccgctatg cgaaggcggc cgagccgatc gccgcgcgcg
      721 ccgtggggcg gctcgccggg ccggccctga agaccaacaa cgagcatggc gagtccgtgc
      781 tcggcaacct catcgccgac gcccagctgg cggcgacccg gggcgcggcc aacggcaacg
      841 cccggatcgc tctcatgaac ccgggcggga tccgggccga tctggtgccg gccgcggacg
      901 gcgggatcag cttcggccag ctcttcgcca cccagccctt tgccaacatg atggtggtga
      961 aggccctcac gggacgtcag ctgcgggccg tgctcgagca gcagttcgcg agcggctcga
     1021 acaccgccgc caacccctac atcctctcgc cctcgcacaa tctgcgcttc gctttcgatc
     1081 tgtcgaagcc ggaggggcag cggatcgtgc tcctgacggt ggatggccag ccggttcgcg
     1141 acgaggcggt ctaccgggtg gcggcgaaca acttcctcgc cggcggcggc gacaatttca
     1201 ctgttttcag ggagggaacg gactcgaccg gcggagtgca ggatctcgac gccctcgaag
     1261 cctatatcgc cgcggcgggg aacctcgctc cgccggcgct tgggcgggtg gagaaccgga
     1321 ctccgaggcg ataagccccg ccccgtcatt gcgaggagcg aagcgacgaa gcaagccagc
     1381 gggcgtccga agcagcaccg gattgcttcg cttcgctcgc aatgacggac cctacctgaa
     1441 cggcggctcg tcgaagcttc ggagtttgcg gctgtgcaga ttgccgccct ggttcttgag
     1501 gatgcgcagg gtctcgatcc cgatcctcag atgctggctg atcgcccgct cgtagaaggc
     1561 gttggcctgg ccggggagct tgatctcgcc gtggagcggc ttgtcggaga cgcagagcag
     1621 ggtgccgtag gggacgcgga agcggtagcc ctgggcggcg acggtggcgg attccatgtc
     1681 gatggcgacg gcgcgcgact ggttgaagcg caacgcggag gactggatcc gaagctccca
     1741 gttgcggtcg tcggtggtga cgacggttcc ggtccggagg cgccgcttca gctcctgagg
     1801 ctcctccccg gtcacttccg cggcggcgtc gtagagggcc tgctggatct cggcgatggg
     1861 cgggatcggg atctcgaccg gaagggtgcc gtcgaggaca tggtcgtcgc gcagataagc
     1921 gtgggcgagg acatagtcgc cgatcgtctg gctcggcctc aggcccccgc aatggccgat
     1981 catcagccag acctcggggc ggaggacggc gagatggtcg cagatcgtct tggcgttgga
     2041 cgggccgact ccgatgttga cgagggtgat gccgccgcca ttggccgcga tcagatggta
     2101 ggccggcatc tgcaccttgc gccagttgcc ggcggcgact tcggcgacgg gatcggccgt
     2161 gtcgcgggtg accagcacgc ctcctggggc cgaaagcgcc tcgtaggggc tgtcctcgcg
     2221 cttcagctcc tcgaccgccc atcggacgaa ctcgtcgacg tagcggacgt agttggtgaa
     2281 gagcaggtag cgctgcgtgt gctcgaccgg ggtgccggta taatggcgca ggcgggcgag
     2341 gctgaagtcg accctggggc cgtcgaacaa ggcgagcggc cggtcggcgt ggcggctgta
     2401 gtcccaggtg ccgtccgcca cctcgtcgcc gatatgggag agctcggtcg ccgggaacca
     2461 gcggctgagg tcggcggcgt gggcgccgtc gagcctgagg tcgtcggtgc cgtcgaggac
     2521 gtagggataa ggaatctcgc tcgccgagag gccgaccgag atatccacct tatagtcgcg
     2581 gacgagatat tcgagctgct cgaccagata tttgcggaag tggcgcggcc gggcgatgct
     2641 gatcgcgtag cgcccgggct ggttgaggcg cccgaaggcg cgggggggga agctgggcgg
     2701 ctggtcgaag ctgtagtcga tgcgaagctc gggataggcg aacagccccc gcgcccgggc
     2761 ctctgcgtcc ggccgctcgc cgcgaagcag atagcgggcg agcgccccgc gcaaggccgc
     2821 cacggattcg tcgaacatct cgcaaagccg gtcgatgatc gcggggatgg attcgaggtc
     2881 gctcatctcc cctccccttc gcacggcttt gggtgcgggg ctagagggcc ggaagtgaca
     2941 gtctcgttga cagtctcgtt gacagtcacg gtgacagtta cggtgactgt caccgggggc
     3001 tcgtcaccgg gggctcggcg cagggctaat ccggtggggc cgtcattgcg aggagcgaag
//
LOCUS       DASWNU010000410         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_353120, whole genome shotgun sequence.
ACCESSION   DASWNU010000410 DASWNU010000000
VERSION     DASWNU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353120"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /gene="ftsH"
                     /locus_tag="VGB57_08105"
     CDS             <1..1615
                     /gene="ftsH"
                     /locus_tag="VGB57_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY0131354.1"
                     /translation="WLILLYQSLPFLLILGIAFFIMRQMQKNAGSGAMGFGRSKAKLL
                     TEKSGRVTFDDVAGIDESREELQEIVDFLKDPTKFARLGGKIPKGALLVGAPGTGKTL
                     LARAVAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGR
                     HRGAGLGNGNDEREQTLNQLLVEMDGFEANEGIIIIAATNRPDVLDPALLRPGRFDRQ
                     VVVPRPDIEGREKILAVHMKKVPLAPDVDPRTIARGTPGFSGADLANLVNEAALLAAR
                     KGKRLVAMSEFELAKDKVMMGAERKSMVMTEDEKKATAYHEAGHALVSLHVPGCDPLH
                     KVTIIPRGRALGVTWNLPERDRYSMSMKQMKARLALCFGGRIAEQLIYGQDELNTGAS
                     NDIQQATDMARSMVMEYGMSEKLGWLRYRDNQEEVFLGHSVARSQSVSEETARLIDSE
                     VRRLIEEAEAAARKVLTDNLDELHRLAGALLEFETLNGEEAKKAIKGEDIGREDPGSR
                     ATLIPASGTSIPSTRRPRGGIGGPAPQGA"
     gene            1785..>2034
                     /locus_tag="VGB57_08110"
     CDS             1785..>2034
                     /locus_tag="VGB57_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131355.1"
                     /translation="MKFKLLAAALLAVVPLGAAQAMDVATFLIKADALEKKGMLALMS
                     SDYKLLMGEIRSQGAALRAERLAAERAGRKGAYCPPAKQ"
BASE COUNT          362 a          686 c          675 g          311 t
ORIGIN      
        1 gtggctgatc ctgctctacc agtcgctgcc tttcctcctc atcctcggaa tcgccttctt
       61 catcatgcgc cagatgcaga agaacgcggg ctccggggcg atggggttcg gccgctccaa
      121 ggccaagttg ctgaccgaga aatcggggcg ggtgacgttc gacgacgtcg ccgggatcga
      181 cgagagtcga gaggaattgc aggagatcgt cgacttcctc aaggacccga ccaagttcgc
      241 ccggctgggc ggaaagatcc ccaagggggc gcttctcgtc ggcgctccgg gaaccggcaa
      301 gactctgctg gcgcgggccg tcgccggcga ggcgaacgtt cccttcttca ccatctcggg
      361 ctcggacttc gtcgagatgt tcgtcggcgt cggcgcgagc cgcgtccgcg acatgttcga
      421 gcaagccaag aaaaacgccc cctgcatcgt cttcatcgac gagatcgacg cggtcgggcg
      481 gcatcgcggc gcggggctcg gcaacggcaa tgacgagcgc gagcagacct tgaaccagct
      541 cctcgtcgag atggacggct tcgaggccaa tgaaggcatc atcatcatcg ccgcgaccaa
      601 ccggcccgac gtgctcgatc cggcgcttct gcgtcccggc cgcttcgacc gccaggtcgt
      661 cgtgccgagg cccgacatcg aggggcgcga gaagatcctc gccgtccaca tgaagaaggt
      721 gccgctcgcc cccgacgtcg atccgcgcac catcgcccgc ggaactccgg gattctccgg
      781 cgccgacctc gccaacctcg tcaacgaggc cgccctcctc gccgcccgca agggcaagcg
      841 cctcgtcgcc atgtcggaat tcgaactggc caaagacaag gtcatgatgg gcgccgaacg
      901 caagtccatg gtgatgaccg aggacgagaa gaaggcgacg gcctatcacg aggccggcca
      961 tgccctcgtc tcgctccacg tgccgggctg cgacccgctt cacaaggtga cgatcatccc
     1021 gcgcggccgg gcgctgggcg tgacctggaa cctgcccgag cgcgaccgtt actcgatgtc
     1081 gatgaagcag atgaaggccc gccttgctct ctgcttcggc ggccggatcg ccgagcagct
     1141 catctacggc caggacgaat tgaacaccgg cgcgtccaac gacatccagc aggccaccga
     1201 catggcccgc tcgatggtga tggaatatgg catgtccgag aagctcggct ggcttcgcta
     1261 ccgcgacaat caggaagaag tgttcctcgg ccacagcgtg gcacggagcc agagcgtctc
     1321 cgaggagacg gcgcgcctca tcgacagcga ggtccgccgg ctgatcgagg aggccgaggc
     1381 ggccgcccgc aaggtcctca ccgacaatct cgacgagctc caccgcctcg ccggcgcgct
     1441 gctcgaattc gagaccctga acggcgagga ggcgaagaag gcgatcaagg gcgaggatat
     1501 cggccgtgag gacccgggtt cgcgcgcgac gctcattcct gcctcgggta cgtccattcc
     1561 ctcgacccgg cgcccgcgcg gcggcatcgg cggaccggcg ccccaggggg cttaggcccg
     1621 gcggagcggt ggcgcccgtc atcgcggcga aagccggggc ctcgggacaa gaagactcgg
     1681 tgggtctccc gaggtcccgg catttgccgg atgacgggta gcgttcaacc ggcgttcagg
     1741 cgcccggcgc tcctttaccg gggcaatctc gaaggggaaa atcgttgaag ttcaagctgc
     1801 ttgccgccgc cttgctggcc gtggtcccgc tcggcgcggc ccaggcgatg gatgtcgcca
     1861 ccttcctcat caaagccgac gccctcgaga agaagggcat gctggccttg atgtcgagcg
     1921 actataaatt gctcatgggc gagatccgca gccagggcgc ggcgcttcgg gccgagcggc
     1981 tggcggcgga gcgggccggc cgcaagggcg cctattgccc gcccgccaag cagt
//
LOCUS       DASWNU010000411         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_431299, whole genome shotgun sequence.
ACCESSION   DASWNU010000411 DASWNU010000000
VERSION     DASWNU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431299"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..495)
                     /locus_tag="VGB57_08115"
     CDS             complement(199..495)
                     /locus_tag="VGB57_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEY0131356.1"
                     /translation="METRAPRPAQVIGPLGEPLTLDSLPPPSTTRWVVRRKAEVVAAV
                     NGGLLSVDEACARYGLTLEEFASWQRAVDRSGMPGLRVTRIQHYKSLYERQQKF"
     gene            complement(608..877)
                     /locus_tag="VGB57_08120"
     CDS             complement(608..877)
                     /locus_tag="VGB57_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131357.1"
                     /translation="MDLSFFRRAEQRFASRAPARLDFGMLVATLRTQRDSAPAASARS
                     AASAPAASCPDPIAEILLAGPAWREAVAAQRGCFNPAYARQEAVN"
     gene            1083..2198
                     /gene="mnmA"
                     /locus_tag="VGB57_08125"
     CDS             1083..2198
                     /gene="mnmA"
                     /locus_tag="VGB57_08125"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0131358.1"
                     /translation="MIADFQLDRPASEARIVVAMSGGVDSSVVAALAARTGAEVIGIT
                     LQLYDHGEAIGRSKTCCAGRDIYDARTVAERLGIAHYVFDYETRFRDSVIERFADEYA
                     RGRTPIPCVSCNQSVKFTDLLALARDLGADCLATGHYVRRVVGPRGPELHRAADPARD
                     QSYFLFATTREQLDFLRFPLGGLPKPAVREIARELGLIVADKPDSQDICFVPDGDYAS
                     VVKKVRPEAARPGEIVDMEGRRLGEHRGLIHFTVGQRRGLEIGGQAEPLYVVRLEPEA
                     RRVVVGPKEALAVRAARLSGLNWLGEEQSDGLAAKVRSMARPAPARFDGDEIRFEAPE
                     FGVAPGQAAVIYEGSRVLGGGWIEETLPFRAESLAAA"
     gene            complement(2117..3487)
                     /locus_tag="VGB57_08130"
     CDS             complement(2117..3487)
                     /locus_tag="VGB57_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY0131359.1"
                     /translation="MTATKDEPLRRDIGFVGSAFLSFNGMVGAGIFALPGTLYDRFGD
                     FSPFLFPLFGLLVLVVALPFARVAARHPVSGGPVVYAAAFGPAVAFQAGWVYYVARAT
                     ALAANLTVLVTYLAVLWPPLGDGPARAAIIVAVCGLLVWINIVGVKRAVQLVDALTLL
                     KAAPLLFVAVMGLVVAGGTIEAPSGVPPLSDLEAAALLILYAFVGFENSVTPAGETSN
                     PTRNIPRALIATIVGTALLYFVVQLSYVAVMEPGGGGDAPLVELGKALMGPAGGLLLV
                     AAAVFSLLGNISGGLTGTSRTSYAMGRDGLLPGWFGGVSARFATPANSIAFMGALIAL
                     LALSGSFVWLAVVSTLARMFVYSISIASLAKPVVPAKAGTSGQEVSAGLPEVPASAGM
                     TVWLMIVPAIAVCAWAAAQTAWPSWRMLLILVAAGTMLYAAARLSARKGSVSSIQPPP
                     STLDPS"
     gene            3702..>3894
                     /locus_tag="VGB57_08135"
     CDS             3702..>3894
                     /locus_tag="VGB57_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131360.1"
                     /translation="MRRLVTIDLPRLLSRRLPRPVTELAVGIGITAIFVGLRLAIAPL
                     VGDAAPFALSIIAVLLAALV"
BASE COUNT          644 a         1343 c         1355 g          552 t
ORIGIN      
        1 gggggtgatc aaggtgaagt cgagggattt ccattagtcc tccccatgcg aagcatgggg
       61 agggggacca tgtgcaacat ggtggagggg taaatggctc ggaatccgcg gatagagcgc
      121 gggacggaag aagcccgcgc cttctacccc tccaccccgc tacgcggggt ccccctagcc
      181 atctccgtgg ggaggatttt agaacttctg ctggcgctcg tagaggctct tgtaatgctg
      241 gatccgggtc acccgaaggc ccggcattcc ggagcggtcg acggcgcgct gccaggaggc
      301 gaactcctcc agcgtgaggc cgtagcgcgc gcaggcctcg tcgacgctga gcagcccgcc
      361 attgaccgcg gcgaccacct cggccttgcg gcgcacgacc cagcgggtcg tcgaaggcgg
      421 cggcagcgaa tcgagagtca gcggctcccc caaagggccg atgacctgcg ccgggcgcgg
      481 cgcccgggtc tcgatcgtgt cgtattcaag gacgttcacg gacattttat ctcctgcctc
      541 cctcagcccc gcttagcggc gggcgttgaa ggaaggctga aaccccaagg taaacgcggc
      601 ggtcatctca attgacggct tcttgccggg cataagccgg attgaagcag ccgcgctgcg
      661 ccgccaccgc ctcgcgccag gctggcccgg cgaggaggat ctcggcgatc ggatcgggac
      721 aagacgccgc cggcgccgaa gccgccgacc gagccgaagc cgccggcgcc gagtcgcgct
      781 gagtgcgcaa cgtggcgacc agcattccga aatccaggcg cgcgggcgcc cggctcgcaa
      841 acctctgctc ggcccgtcgg aagaagctca aatccatggc cgtccttcta gccgcgaggg
      901 tctaagacgc ggttaaaggc ccattaggac gggctttacc cgggttaatg ccccacggcg
      961 cctgctcccc ggcgaaggcc ggggcccagg ctaccggcgg tgcgactgga tcccggcctt
     1021 cgccgggaaa cattcgccta aagctgggct ttactccctg acgcccctat gtagccgcgg
     1081 tcatgatcgc cgatttccag ctcgaccggc cggccagcga ggccaggatc gtcgtcgcca
     1141 tgtcgggcgg agtcgacagc tcggtcgtcg ccgcgctggc ggcccggacg ggcgccgagg
     1201 tgatcgggat caccctccag ctctacgacc atggcgaggc gatcggccgc tccaagacct
     1261 gctgcgccgg ccgcgacatc tacgatgccc gcacggtcgc cgagcggctc ggcatcgccc
     1321 attacgtctt cgactacgag acccgcttcc gcgacagcgt gatcgagcgc ttcgccgacg
     1381 aatatgccag gggccgcacc ccgatcccct gcgtgtcgtg caaccagtcg gtcaagttca
     1441 ccgaccttct cgccctggcc cgcgacctgg gcgccgactg cctcgccacc ggccattacg
     1501 tccgccgggt cgtcgggccg cggggccccg agcttcaccg cgccgccgat cccgcccgcg
     1561 accagagcta tttcctgttc gcgaccaccc gcgagcagct cgatttcctg cgcttcccgc
     1621 tcggcggcct gcccaagccc gcggtgcgcg agatcgcccg agagctcggc ctcatcgtcg
     1681 ccgacaagcc ggacagccag gacatctgct tcgtgcccga cggcgattat gcctcggtcg
     1741 tgaagaaggt ccggcccgag gcggcgcggc ccggggagat cgtggacatg gagggccgcc
     1801 ggctcggcga gcatcgcggc ctcatccatt tcaccgtcgg ccagcgccgc gggctcgaga
     1861 tcggcggcca ggccgagccg ctctacgtcg tccggctcga gccggaggcg cggcgggtcg
     1921 tggtcggccc gaaggaggcg ctggcggtac gcgccgcccg cctctcgggc ctcaactggc
     1981 tcggcgagga gcaaagcgac ggactcgccg ccaaggtgcg gtcgatggcg cgcccggcgc
     2041 cggcccgctt tgacggcgac gagattcgtt tcgaggcgcc ggaattcgga gtagcgcccg
     2101 gtcaggcggc ggtgatctac gaggggtcga gggtgctcgg cggcggctgg atcgaggaga
     2161 cgctgccctt ccgggccgag agcctcgccg ccgcgtagag cattgtgccc gccgcgacga
     2221 ggatgagaag catccgccag ctcggccagg cggtctgggc ggcggcccac gcgcagacgg
     2281 cgatggcggg gacgatcatc aaccacaccg tcatcccggc ggaagccggg acctcgggga
     2341 gacccgccga gacttcttga cccgaggtcc cggctttcgc cgggacgacg ggcttggcca
     2401 aagacgcgat gctgatcgaa tagacgaaca tccgcgccag cgtcgacacc acggccagcc
     2461 agacgaagct gccgctgagc gcgagcaatg cgatcagggc gcccatgaac gcgatcgaat
     2521 tggccggagt ggcgaagcga gcgctgacac cgccgaacca gccgggcaga aggccgtccc
     2581 ggcccatggc gtagctcgtt cgcgacgtgc cggtcagccc gccgcttatg ttgccgagca
     2641 gcgagaagac cgccgctgcg acgagcagca gccctccggc cggccccatc agcgccttgc
     2701 cgagctcgac caggggcgcg tcgccgcccc cgccgggctc catcaccgcc acgtaggaga
     2761 gctggacgac gaagtagagc agcgccgtgc cgacgatggt ggcgatgagc gcgcgcggga
     2821 tgttgcgcgt agggttggac gtctcccccg ccggggtcac gctgttctcg aaaccgacga
     2881 aggcgtagag gatgagcagg gccgcagcct cgaggtcgga gagcggcggc acccccgacg
     2941 gagcctcgat cgtcccgccc gccacgacca ggcccatgac cgccacgaac agcaaaggcg
     3001 ccgccttgag cagggtcagg gcgtcgacaa gctgcaccgc gcgcttcacc ccgacgatat
     3061 tgatccagac gagcaggccg cagacggcga caatgatcgc agcccgcgcc gggccgtcgc
     3121 cgagcggcgg ccaaagcacg gcaaggtagg tgacgagcac cgtcaggttg gcggccagcg
     3181 cggtggcccg ggcgacataa tagacccatc ccgcctggaa ggcgacggcg gggccgaagg
     3241 cggcggcata gacgacgggc ccgccggaga cgggatgacg cgccgcaacc ctggcgaagg
     3301 gtagagcgac gacgaggacg agaaggccga acaagggaaa gaggaagggg ctgaagtcgc
     3361 cgaaccggtc gtagagcgtg cccggcaggg cgaagatgcc ggccccgacc attccattga
     3421 acgacaggaa ggcggagccg acgaagccga tgtcgcgccg gaggggttcg tccttggtcg
     3481 cggtcacaca tccccccgtg cctggttcgg gaacggttct gactgcgctg cccctccccc
     3541 gcaagggctt agtaattggt gccaggcacc gattttcgga cggcctgtgg ccgggccgcc
     3601 gcacttgccg cgcgatttgg ccggctgatt tcgatcagcg cggttgcggt ggccgctgcc
     3661 cgctctcctg tggtagcaac cccgcgggag ttgggggtag attgcgcagg ctcgtcacca
     3721 tcgatctgcc gcggcttttg tcgcgacggc tgccgaggcc ggtaacggag ctcgccgttg
     3781 gaatcggcat caccgcgatc ttcgtcggcc tgcgcctcgc gatcgcaccg ctggtcggcg
     3841 acgcggcgcc cttcgccctg tcgatcatcg ccgtcctgct cgcggccctg gtcg
//
LOCUS       DASWNU010000412         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_455769, whole genome shotgun sequence.
ACCESSION   DASWNU010000412 DASWNU010000000
VERSION     DASWNU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455769"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="VGB57_08140"
     CDS             <1..1669
                     /locus_tag="VGB57_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0131361.1"
                     /translation="GAKQRLLVPPGPNGSTHDLQGIDVQGAVAGAIAKAVTGAYLSSA
                     GNAFSTRTASMIVQEHFPPSAVTPGLESGPLFGVQFSSLPCSDLSARFGVPGPGALVG
                     PKRSPLGLAADPGGFPLYRNGVVIGGVGIMADGQYGFDPEVRDVDNDAEEAIALAATR
                     GYEAPVEIRAERVSVDGTLLRYSDADPGDFASGTVLPFAAINGTAGVLRTVRGYYDST
                     GGILAGAAYGTEASGVRPANPAEFSNSDAFVLTDGAGSNRFPIRGGTETGIAPLSGSD
                     VRALLEEAFTIMTAARAQIRRPLDSRAQVTISVVDTNGAVLGIVRSPDAPIFGIDVSL
                     QKARTAMFFSHPAAETQLLANPDADVRGFVAAQHAFFGSFAAFSGQIAWSARAIGNIH
                     RPYFPDGEVGRPPGPLSRAIAEFNPFSTGLQSALIEQDVRTHVGFILGGASDVPQQCG
                     SAAPGPDRTANGIQIFPGGVPIYRNGTLVGGIGVSGDGIDQDDMISFLGAHNGGVRTG
                     FFGNADPAIRSDQVVVRVGGNDVRLRYVGCPFAPFLGSDGQNVCQGR"
     gene            1792..>3201
                     /locus_tag="VGB57_08145"
     CDS             1792..>3201
                     /locus_tag="VGB57_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131362.1"
                     /translation="MLSAAVFMDSRFRGNDGSAQEQPAAEPGWWPGQSPPADRPAEQV
                     EDPEGRRRPGVPQHDLPDPVVQDNPGAIRSPPPEAFPADQIPVPDRWRLIESLGVVRE
                     RWWDPYNQNTLKGDRPLCVGEERPHSWGCRLQHALGLHGDDWFLAVNAVSDTVIEPRT
                     FPIPVGVQTTQRPGSLDVFGRSESLVLAQTFIVGAALIQGSTAYMPPHREYRVTLAVQ
                     ANYVDVPERRILSVRPSDPSHRLDYALGVQELFVDDHLRNVSDRFDFDSIRIGIQPFQ
                     FDFRGFLFNDNQLGVRLFGNRDNNRFQYNVAAVWRLEKDTNSGLNNILQWPRRDWILH
                     ANLFRQDFPFVGMTSQASVTLNVNRERGDVEVDDNGFPVRPALIGNLRGRNYEVAYLG
                     YAADGRIGRINLSAQLYGAFGWDRKSVFTGETARISSFFAAAEPSYDMNWIRLRGAAL
                     YASGDGDPYDNSHHGFDSIF"
BASE COUNT          486 a         1173 c         1021 g          521 t
ORIGIN      
        1 cggcgcgaaa cagcggctgc tcgtgccgcc ggggccgaac ggatcgaccc acgatctgca
       61 gggcatcgac gtccagggcg ccgtcgccgg ggcgatcgcc aaggcggtga ccggtgccta
      121 tctttcctcc gccggcaacg ccttttcgac gcgcacggcg agcatgatcg tccaggagca
      181 tttcccgccc tcagcggtca ccccgggact ggagagcggg cccttgttcg gagtccagtt
      241 cagctcgctg ccctgctccg atctcagcgc caggttcgga gtgccggggc ccggcgcact
      301 ggtcggcccg aagcgctctc cgctcggcct cgccgccgat cccgggggct tcccgctcta
      361 ccgcaacggg gtggtgatcg gcggcgtcgg gatcatggcg gacggccaat acggcttcga
      421 tcccgaggtc cgcgacgtcg acaatgacgc ggaggaagcg atcgccctcg ccgccacccg
      481 cggctacgaa gcccccgtcg agatccgcgc cgagcgggtc agcgtcgacg gcacgctgct
      541 gcgctacagc gacgccgacc cgggcgattt tgcgagcggc acggtcctgc ccttcgcggc
      601 gatcaacggc accgccggcg ttctgaggac ggtgcgcggt tattacgact caaccggcgg
      661 aatcctcgcc ggtgcggctt acgggacgga ggcatccggc gtccgccctg caaacccggc
      721 ggaattctcg aacagcgacg ccttcgtcct caccgacggc gccggcagca accgcttccc
      781 gatccgcggc ggcaccgaga cgggcatcgc tccgctgagc ggctccgatg tccgtgcctt
      841 gctcgaggag gctttcacga tcatgaccgc ggcgcgcgcg cagatccgcc gcccgctcga
      901 cagccgcgcc caggttacca tctcggtggt cgacaccaac ggggccgtgc tcggcatcgt
      961 ccgctccccc gacgcaccca tcttcggcat cgacgtctcg ctgcagaagg cccgcacggc
     1021 gatgttcttc agccaccccg ccgcggagac ccagcttctc gccaatcccg acgcggacgt
     1081 gcgcggcttc gtcgcggccc agcacgcctt cttcggcagc ttcgccgcgt tcagcgggca
     1141 gatcgcctgg tcagcccgcg caatcggcaa catccaccgc ccctatttcc ccgacggcga
     1201 ggtcgggcgc ccccccgggc cgctctctcg ggcgatcgcc gagttcaatc ccttctcgac
     1261 cgggctgcaa tcggcgctga tcgagcagga cgtgcgcacc catgtcggct tcatcctcgg
     1321 cggcgccagc gacgtcccgc agcaatgcgg cagcgccgcg ccggggccgg accgcaccgc
     1381 gaacggcatc cagatcttcc cgggcggcgt gcccatctac cgcaacggca ctttggtggg
     1441 cgggatcgga gtctcgggcg acggcatcga ccaggacgac atgatctcct tcctcggcgc
     1501 ccataatggc ggcgtcagga ccggcttctt cggcaatgcg gacccggcga tccgctccga
     1561 ccaggtcgtc gtccgcgtcg gcggcaacga cgtgcggctg cgatatgtcg gctgcccgtt
     1621 cgcgccgttc ctcggctccg acggccagaa tgtctgccag ggccgctgag ggtggcgctg
     1681 gcggctccct tcgtctccct actttcctcg gccggactgg gcgcggcgta cctgccgccc
     1741 tcaatccgtc atcccggact tgatccggga tccatgaaca ccgccggtgc catgctgtct
     1801 gccgcggtgt tcatggattc ccgctttcgc gggaatgacg ggtccgcgca ggagcagccg
     1861 gccgccgagc cgggctggtg gcccggacaa tcgcctcccg ccgatcgccc tgccgagcag
     1921 gtcgaggacc ctgaaggccg tcgtcgcccc ggcgtgccgc agcacgacct ccccgatccg
     1981 gtcgtgcagg acaatccagg cgcgatccgc tcgccgccgc ccgaagcctt tccggccgat
     2041 cagatcccag tcccagaccg ctggcggctg atcgagtcgc tcggcgtcgt gcgcgaacgc
     2101 tggtgggacc cctacaatca gaacacgctc aagggagaca ggccgctctg cgtcggcgag
     2161 gaaaggccgc acagctgggg ctgccgcctc cagcacgccc tcggcctgca tggcgacgac
     2221 tggtttctgg cggtgaacgc cgtctcggac acggtgatcg agccgcgaac cttcccgatc
     2281 ccggtaggcg tgcagacgac gcagcggccc ggcagcctcg acgtgttcgg gcgcagcgag
     2341 agcctcgtcc tcgcccagac cttcattgtc ggcgcggcgc tcatccaggg cagcacggcc
     2401 tacatgccgc cccaccgcga atatcgcgtc acgctcgccg tccaggccaa ttatgtcgac
     2461 gtccccgagc gccgcatcct ctcggtgcgg ccttcagacc ccagccaccg gctagactat
     2521 gccctcggcg tccaggagct gttcgtcgac gaccatctgc gcaacgtctc cgaccgcttc
     2581 gacttcgatt cgatccgaat cggcatccag cccttccagt tcgatttccg cggcttcctg
     2641 ttcaacgaca accagctcgg cgtgcgcctg ttcggaaacc gcgacaacaa tcgcttccag
     2701 tacaatgtcg ctgcggtctg gcggcttgag aaggacacca attccgggct caacaacatc
     2761 ctgcaatggc cgcgcaggga ctggatcctg cacgcgaacc tgttccgcca ggatttccca
     2821 ttcgtgggca tgacgagcca ggcgagcgtt acgttgaacg tcaaccgcga gcgcggggac
     2881 gtggaggtgg acgacaatgg ctttccggtg cggcccgcgc tgatcggcaa tttgcgcggt
     2941 cgcaattacg aggtcgccta tctcggctat gcggcggacg ggcggatcgg acggatcaac
     3001 ctctcggcgc agctctacgg tgcgttcggc tgggaccgga agagcgtctt caccggcgag
     3061 accgcgcgga tttcgagctt cttcgccgcg gcggagccga gctacgacat gaactggatt
     3121 cgcctgcgcg gcgcggcgct ctatgcgagc ggcgacggcg acccgtacga caacagccat
     3181 cacggcttcg actcgatctt c
//
LOCUS       DASWNU010000413         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_16520, whole genome shotgun sequence.
ACCESSION   DASWNU010000413 DASWNU010000000
VERSION     DASWNU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16520"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGB57_08150"
     CDS             complement(<1..192)
                     /locus_tag="VGB57_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEY0131363.1"
                     /translation="MTDTPEAAPEAAPAAKKEGTDWWAELKAIFWLILAVLAFHSFIA
                     KPFYIPSESMMPGLLKGDRL"
     gene            complement(210..626)
                     /gene="acpS"
                     /locus_tag="VGB57_08155"
     CDS             complement(210..626)
                     /gene="acpS"
                     /locus_tag="VGB57_08155"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY0131364.1"
                     /translation="MIIGIGSDLCNISRIENSLSRFGERFTNRVFTDAERARAEGRPL
                     TRAGTYAKRFAAKEAFSKAVGTGFKRGVFMRDIGVVNLPSGAPTLALTGGAKARLDAL
                     TPEGHLAQIHLTMTDDYPFAQAFVIITSSPLPRSPG"
     gene            complement(721..1452)
                     /locus_tag="VGB57_08160"
     CDS             complement(721..1452)
                     /locus_tag="VGB57_08160"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY0131365.1"
                     /translation="MTGPLRLGVNIDHVATIRNARGGDFPDPVRAALLAAEAGADGIT
                     AHLREDRRHIRDEDISKLMERLTIPLNLEMAATEEMLEIALRHRPHAACIVPEKREER
                     TTEGGLDAAGQDNHLMPFIERLSGANIRVSLFIEPDPRQIEAAIRLKAPVVELHTGRY
                     AHLEGEARAEELRRLADAAALASKNGIEPHAGHGLTFDNVQPVAAIPQLRELNIGHFL
                     VGEAIFTGLGESVGRMRSLMDEARG"
     gene            complement(1449..2033)
                     /gene="pyrE"
                     /locus_tag="VGB57_08165"
     CDS             complement(1449..2033)
                     /gene="pyrE"
                     /locus_tag="VGB57_08165"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0131366.1"
                     /translation="MDEEEILAEFRAAEALLEGHFILSSGLHSPRYLQCARVLMDPAR
                     ASRLAHAIATALPLEIRSRIEAVVSPAMGGIIIGHEMGRSLGLEAMFLERPAGTFEFR
                     RGFRLAPGQKVLLVEDVVTTGLSSREAMRAIEAAGAEVIAAASLVDRSNGLADLGVPF
                     FPLIRLDIPVYEPEALPSELAALAVEKPGSRKAA"
BASE COUNT          362 a          778 c          834 g          389 t
ORIGIN      
        1 cagccggtcg cccttgagaa gacccggcat catcgattcg gacgggatgt agaagggctt
       61 ggcgatgaag ctgtggaagg ccagcacggc caggatcagc cagaagatgg ccttgagctc
      121 cgcccaccag tcggtgccct cctttttcgc cgcgggcgcc gcctccgggg cagcttcggg
      181 cgtgtcggtc atggcggggt cgctgctgct caccctgggc tccttggcaa cgggctggag
      241 gtaatgatga cgaaggcctg cgcgaaagga tagtcgtccg tcatcgtcaa gtggatttga
      301 gcgaggtggc cctccggagt caaagcgtca agccttgcct tggcgccgcc ggtcaaggcg
      361 agggtcggcg ctcccgacgg caggttgacg actccgatgt cccgcatgaa cacgccgcgc
      421 ttgaaaccgg tgcccaccgc cttggaaaac gcttccttgg cggcgaagcg cttggcgtag
      481 gtgccggcgc gggtgagggg acggccttcg gccctggcgc gctccgcgtc ggtgaagacg
      541 cggttggtga agcgctcgcc gaagcgggag agcgaattct cgatccggga gatgttgcac
      601 aggtccgagc cgatgccgat gatcataggc cgtcaccccg gcgaaagccg gggcctcggg
      661 gagtcccgcg gagacttctc gtcctgaggt cccggctttc gccgggatga cggggctgcc
      721 tcatcccctc gcctcgtcca tcagcgaccg catccgcccc acgctttcgc caagcccggt
      781 gaagatcgct tcgccgacga ggaaatggcc gatgttgagc tccctcaatt gcgggatggc
      841 ggcgacgggc tggacgttgt cgaaggtgag gccgtggccg gcatggggct cgattccgtt
      901 cttcgaagcc agagcggcgg cgtcggcgag ccggcgcagc tcctcggcgc gtgcctcgcc
      961 ctcgagatgg gcgtagcggc cggtatggag ctcgacgacc ggcgccttca ggcggatcgc
     1021 cgcctcaatc tggcgcggat cgggctcgat gaacaggctg acccggatgt tggcgccgct
     1081 aaggcgctcg atgaagggca tcaggtgatt gtcctgcccg gcggcatcga ggccgccttc
     1141 ggtggtgcgc tcctcgcgct tctcggggac gatgcaggcg gcatgggggc ggtggcgaag
     1201 cgcgatctcg agcatctcct cggtcgcggc catttcgagg ttcaagggga tggtcagccg
     1261 ctccatcagc ttcgatatgt cctcgtcgcg aatgtggcgg cggtcctcgc gcagatgggc
     1321 ggtgatcccg tcggctccgg cctcggccgc gagcagggcg gcgcgcaccg gatcgggaaa
     1381 atcgccgccg cgcgcattcc tgatggtcgc gacatggtcg atgttgactc cgaggcgaag
     1441 ggggccggtc acgccgcctt gcggctcccg ggcttctcga ctgccagggc cgccagctcc
     1501 gacggaagcg cctccggctc gtagacgggg atgtcgagcc tgatcagcgg gaagaaaggc
     1561 acgccgagat cggcgaggcc attcgaccga tcgacgagcg aggcggcggc gatcacctcg
     1621 gcaccggccg cctcgatcgc gcgcatcgcc tcgcgcgagc ttaggccggt ggtgacgacg
     1681 tcctccacca gcagcacctt ttgacccggg gcgaggcgga agccgcggcg gaactcgaac
     1741 gtccccgccg gtcgctcgag gaacatcgcc tcgaggccga gggagcggcc catttcgtgg
     1801 ccgatgatga tcccgcccat cgccggggag accaccgcct cgatccggct ccgtatctcc
     1861 agcggcaagg cggtagcgat ggcgtgggcg aggcgcgagg cgcgcgcggg atccatcagc
     1921 actcgcgcgc attggaggta gcgcgggctg tgaaggcccg aggagaggat gaaatgcccc
     1981 tcgagcagag cctcggcggc tcggaattcg gcgaggatct cctcttcgtc cataggtcct
     2041 tttctttcgg ttggggccgg cggcgtgata ggccgcgaaa agggcggctt caaggcggtc
     2101 caagagcctt gaggcgcacg gacctcctgc ctataaggcc cgccaatccg ccgccccacc
     2161 tagcgaatcc gttccgcttt cgtcgtccag gtcccgaagg gtttagatga agactccgct
     2221 caacctgatc ctcgccgccg ccgccttcgc gctggctccg gccgccgcgc tctcgcaggc
     2281 ggccccggcc tccgccccgg cgcagacggc acaggctccc gcgcctgccg cccagtcgcc
     2341 ccgggactcg attagcgcca att
//
LOCUS       DASWNU010000414         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_275185, whole genome shotgun sequence.
ACCESSION   DASWNU010000414 DASWNU010000000
VERSION     DASWNU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275185"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VGB57_08170"
     CDS             <1..1148
                     /locus_tag="VGB57_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131367.1"
                     /translation="LAHASRALIGISREMIADAEAVRLTHNPAALVSALRRIEAGKDM
                     GALGDEHDSMLIVGATHGPLATHPTLEERAGALARTTGSLMLATAPRLDTRPLAGRRA
                     PGFGWRPDPALERIAVLAEAPRQRGLWGAFRSVRDPERNLMGLNRRAAAIIAGSLLGI
                     GILYQPSLAHPGAVAKIFDPEMLRPFAGMGATVARCVLAFGATDEELEKCEKDAAEGG
                     RLFEHIPLFAPKDGRPREYLTPRESEARRAAQKVARGCVSGRLAPDGSENLSMDIFRR
                     FGERAPSKSLELPPGPDRDAALVAYALDRLNLIEVSLDSFGEPGFAALRQAVAGDDHE
                     AVLELLRRRLEDREFRRRTGSNTSADIELLVSRPTGLRPCRAKRPTA"
     gene            complement(1236..2960)
                     /locus_tag="VGB57_08175"
     CDS             complement(1236..2960)
                     /locus_tag="VGB57_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY0131368.1"
                     /translation="MPKSLLAAVSAATLLLAGCASTGSEKASTAAQPSAWPAFSQSFI
                     EGYFRISPAFAVNQGKHEYDGQLPDWSETGLANTAEFLRSSIAAAQAFDERRLNKTER
                     FERDYLIARARGDLFWLETADQPRRNPAYYMGNGLDPSVYVTRPYAPAETRLRAFIAY
                     LKAVPRAARQIRANLRTPLPLSFINYGKAGFGGFAEYYPTDGKAAFASVADPELQSEL
                     DSSAREAARAMKGLADWLESERPNATQDFALGADKFARMLRDTEMVDIPLAELEAIGR
                     ADLKRNQDALRSSCARYAPGASLQACMNKMAANKPKGGAVQAARAQLAGLKRFIEEKD
                     LVSIPGTEEARVEESPPYNRQNFAYIDIPGPYERGLPSVYYIAPPDPSWPAEVQAGFV
                     QGEAALLFTSVHEVWPGHFLNFLHSNRSPFTFGRVFVGYAFAEGFAHYTEEMMWDAGL
                     RNGDLETHVGQISNALLRDCRYLSAIGMHTGRMTQEQSRQMFLSECYQDEGNARQQSA
                     RGTYDPAYLNYTMGKLLIRRLREDWTRTRGGRQAWKQFHDKFLSYGGPPIPLVRAQMM
                     GGEAKAVF"
BASE COUNT          508 a         1023 c         1115 g          523 t
ORIGIN      
        1 ggctggctca tgcatcgcga gcgctgatcg gcatatcgcg cgagatgatc gccgacgcag
       61 aggcggtgcg actgacccac aaccccgccg cgctcgtttc cgcccttcgc cggatcgagg
      121 cgggcaagga catgggcgcg ctcggcgacg agcatgactc gatgctgata gtcggggcaa
      181 cccatggccc gctggcgacc catccgaccc tcgaggaacg ggccggagcg ctggcccgga
      241 cgaccggatc gctcatgctc gctacggcgc cccggctcga cacgcggccg cttgcaggcc
      301 gccgtgcccc gggtttcggg tggcgacccg atccggcgct cgagcggatc gcggtcctcg
      361 ccgaagcgcc ccggcagcgc ggattatggg gcgcgtttcg gtccgtacgc gaccccgagc
      421 gcaacctgat ggggctcaat cggcgcgccg ccgcgataat cgcggggtcg ctgctgggaa
      481 tagggatcct gtatcagccc tcgctcgcgc atccgggcgc ggtcgcgaag atattcgatc
      541 cggaaatgct tcgtccgttc gcaggaatgg gcgcgaccgt ggcgcgctgc gtgctggctt
      601 tcggtgcgac cgacgaagag ctggaaaaat gcgagaaaga cgccgcggaa ggtggacgcc
      661 tgttcgagca tatcccgttg ttcgcgccga aggacgggag gccccgtgaa tatctgactc
      721 cgcgcgagag cgaagcgcgc agggccgcgc agaaggtcgc gcggggctgc gtctccggac
      781 gtctcgcccc cgacgggtcc gagaacctct cgatggacat cttccggcgc ttcggcgagc
      841 gtgcgccctc gaaatcgctc gagctgccgc ccgggcccga tcgcgacgcg gcgctggtcg
      901 cctatgcgct ggacaggctg aacctcatcg aagtcagctt ggactcgttc ggcgagcccg
      961 gtttcgccgc gctgcgccag gcggtcgccg gcgacgatca cgaggcggtc ctcgagctgt
     1021 tgcgccgacg gctggaggat cgcgaattca ggcggaggac gggctccaat accagcgccg
     1081 acatcgagct gctcgtttcg cggccgactg gcctcaggcc ctgccgggcg aagcgtccga
     1141 ccgcctagct gcgaccatcg cttcgcggcg tgacaggccg ctacggcccg acgagtcggg
     1201 cttcacggct ttcggaaacc ggtccgggtt ccggttcaga acaccgcctt cgcctcgccc
     1261 cccatcatct gcgcccgcac caacgggatc ggcgggccgc catagctcag gaacttgtcg
     1321 tggaattgct tccacgcctg acgcccgccc ctcgtccgcg tccaatcctc cctcagccgc
     1381 cggatcagaa gcttgcccat cgtgtaattg agataggccg gatcgtacgt cccccgcgcg
     1441 ctctgctgcc gggcattgcc ttcgtcctgg tagcattccg acaggaacat ctgccgcgac
     1501 tgctcctggg tcatcctccc cgtgtgcatc ccgattgccg acaaatagcg gcagtcgcgg
     1561 agcaaggcgt tggagatctg gccgacatgg gtctcgaggt cgccgttcct cagcccggcg
     1621 tcccacatca tctcctcggt ataatgggcg aagccttccg cgaaggcgta gccgacgaag
     1681 acccggccga aggtgaaggg cgagcggttg gaatggagga agttgaggaa atggcccggc
     1741 cagacctcgt gcaccgaggt gaagagcagg gccgcctcgc cctggacgaa ccccgcctgg
     1801 acctccgccg gccaggaagg atcgggcgga gcgatgtaat agacggacgg aaggccgcgc
     1861 tcatagggcc cgggaatgtc gatataggcg aaattctgcc ggttgtaggg cggcgattcc
     1921 tcgacccgcg cctcctccgt tccggggatg gagacgaggt ccttctcctc gatgaagcgt
     1981 ttcagcccgg cgagctgcgc tcgtgccgcc tggacggcgc cgcccttcgg cttgttggcg
     2041 gccatcttgt tcatgcaggc ctgcaggctc gccccgggcg cgtagcgggc gcagctggag
     2101 cgcagggcgt cctggttgcg cttgaggtcc gcccggccga tcgcctcgag ctcggcgaga
     2161 ggtatgtcga ccatctcggt gtcgcggagc atccgggcga atttgtcggc gccgagcgcg
     2221 aagtcctggg tggcattggg ccgctcggac tccagccagt cggccaggcc cttcatcgcc
     2281 cgggccgcct cccgagccga ggaatcgagc tccgactgca actcggggtc ggcgacggag
     2341 gcgaaggcgg ccttgccgtc cgtgggataa tattcggcga agcctccgaa gccggccttg
     2401 ccgtaattga tgaagctgag cggcagcgga gtcctgagat tggcccttat ctgccgcgcc
     2461 gcccggggca ccgccttcag ataggcgatg aaagccctca atcgggtctc ggccggcgca
     2521 taggggcggg tgacgtagac cgacggatcg agcccgttgc ccatgtaata agccgggttg
     2581 cggcggggct ggtcggccgt ctcgagccag aagaggtcgc cccgggcacg ggcgatcaga
     2641 tagtcgcgct cgaagcgctc ggtcttgttg aggcgcctct cgtcgaacgc ctgtgccgcc
     2701 gcgatcgacg agcgcaggaa ctcggcggta ttggcgaggc cggtctcgct ccagtcgggc
     2761 agctggccgt catattcgtg cttgccctgg ttgacggcga aggccgggct gatccggaaa
     2821 tagccctcga tgaagctttg cgagaaagcc ggccaggcgg agggctgcgc ggccgtagac
     2881 gccttttccg aaccggtgga agcgcagccg gcgagcagaa gggtggcagc ggaaacggcg
     2941 gcgagcaggg acttgggcat cggcgactcc tggattgccg tcgaggatta gcctgtcgcg
     3001 gtcatgatga cgaggtcgcc cgtcatcccg gcggaagccg ggacctcagg acgagaagtc
     3061 tcggcgggtc tccccgaggt cccggtgtaa ggggacaaga cattctagag tgcgggaggc
     3121 gggatttgct gggatttgcg gggatatgga gggatggact cggcgccgg
//
LOCUS       DASWNU010000415         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_145390, whole genome shotgun sequence.
ACCESSION   DASWNU010000415 DASWNU010000000
VERSION     DASWNU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145390"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /gene="rseP"
                     /locus_tag="VGB57_08180"
     CDS             complement(<1..1047)
                     /gene="rseP"
                     /locus_tag="VGB57_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY0131369.1"
                     /translation="MNETPGVFWTILSFLLVIGPLIFVHELGHYFVGRWFGVKADAFS
                     IGFGREIAGWTDKRGTRWKLAWLPLGGYVKFAGDMNAAGQPSPEWLALPAEERAKTFQ
                     AKPVWQRFLIVLAGPMTNFLAAILILTGFSIAYGVPRTPAVIGAFTEGSPAAAAGLRA
                     GDRIVRIDGKDIDRFEDILPVVALKIGGPLDLEVEREGRRFQVDVLPQVVVEKDRFGN
                     ENRRPVLGIGAAEQVLTPVSLPGAVAASVRQTWVVVTTTVKGLGQIVTGRRSVKEMGG
                     PLKIAKFSGEQASMGLLAFLWFVAVISINLGFINLLPIPMLDGGHLFFYGIEAVRRKP
                     LRPEAQEWAFRTGLA"
     gene            complement(1044..>1713)
                     /locus_tag="VGB57_08185"
     CDS             complement(1044..>1713)
                     /locus_tag="VGB57_08185"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0131370.1"
                     /translation="GISRIILTASGGPFRDKSLEEMRSATPAEAIAHPNWSMGAKISV
                     DSATLMNKGLELIEAFHLFPLPAEAFDIVIHPQSVVHSLVEYVDGSVLAQLGSPDMRI
                     PIAYTLAWPGRMATPCERLDLVRTATLSFVAPDPERFPALELARQALRDGGARPAVLN
                     AANEVAVGAFLGGAIGFLDIASIAAETLRCYAPAAPGSVEDVLQIDREARASAEAIVK
                     RVQR"
BASE COUNT          303 a          558 c          581 g          271 t
ORIGIN      
        1 agccaagccg gtcctgaacg cccattcctg tgcctccggc cgcaagggtt tccggcggac
       61 ggcctcgatc ccatagaaga acaggtggcc cccgtcgagc attgggattg gcaacaggtt
      121 gatgaatccc agattaattg aaatgacggc gacgaaccac aggaaggcga gaagccccat
      181 gctcgcctgc tcgccggaaa atttggcgat cttgagcggc ccgcccattt ccttgaccga
      241 gcggcgaccc gtaacgatct ggccgagtcc cttcaccgtc gtcgtcacca ccacccaggt
      301 ctgcctgacc gaagcggcga ccgcgcccgg gagcgacact ggagtcagca cctgctccgc
      361 ggctccgatg ccgaggacgg ggcggcggtt ctcgttgccg aaccggtcct tctcgacgac
      421 cacctgcggg aggacgtcga cctggaaccg gcggccctct ctttcgacct cgaggtcgag
      481 cggcccgccg atcttcagag cgaccacggg caggatgtcc tcgaaacggt cgatgtcctt
      541 gccgtcgatt cgaacgatcc ggtcgccggc cctgagtccg gccgcggccg cgggtgagcc
      601 ttccgtgaag gcgccgatca cggccggggt ccggggaacg ccgtaggcga tgctgaagcc
      661 ggtcaggatc aggatggcgg cgaggaaatt ggtcatcgga cccgcgagga cgatgaggaa
      721 gcgctgccag accggtttcg cctggaaggt cttcgcccgc tcctcggccg gcagcgccag
      781 ccattccggc gagggctggc cggccgcgtt catgtcgccg gcgaacttga cgtagccgcc
      841 cagcggaagc caggccagct tccagcgcgt gccgcgtttg tcggtccagc ccgctatctc
      901 ccttccgaag ccgatcgaga aggcatcggc cttgaccccg aaccagcgcc cgacgaaata
      961 atggccgagc tcgtgaacga agatcagcgg accgatgacg agcaggaagc tcaggatggt
     1021 ccagaagacg ccgggagtct cgttcaacgc tgtacccttt tcacgatagc ttcggcggag
     1081 gcgcgggcct cgcggtcgat ctggagcacg tcttcgaccg agccgggagc cgccggcgca
     1141 tagcatcgca gagtctcggc ggcgatggaa gcgatgtcga gaaagccgat tgcgccgccc
     1201 aggaaggcgc cgaccgcgac ttcgttggcg gcgttgagga ccgccggccg ggcgccgccg
     1261 tcgcgaagcg cctgccgggc gagctcgagc gctggaaagc gctccggatc cggagcgacg
     1321 aagctcaagg tcgcggtcct gaccaggtcg agccgctcgc acggagtcgc cattcgcccg
     1381 ggccaggcca gcgtataggc gatcgggatt cgcatgtccg gcgagcccaa ttgggcgagt
     1441 acggatccgt cgacatattc gaccaggctg tggaccaccg actgggggtg gatgacgatg
     1501 tcgaacgcct cggccggcag cggaaagagg tggaaggcct cgatcagctc cagccccttg
     1561 ttcatcaggg tcgcggaatc gaccgatatc ttcgcgccca tcgaccaatt ggggtgggcg
     1621 atcgcctcgg cgggggtggc ggagcgcatc tcctccaggc tcttgtcgcg gaagggcccg
     1681 ccgctggcgg tgaggatgat ccggcttatc cct
//
LOCUS       DASWNU010000416         4540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_275434, whole genome shotgun sequence.
ACCESSION   DASWNU010000416 DASWNU010000000
VERSION     DASWNU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275434"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VGB57_08190"
     CDS             complement(<1..450)
                     /locus_tag="VGB57_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0131371.1"
                     /translation="MKLGIYHGAALMLAGSLAAAAAVAQTVSDPGPAAPSARLNIPAN
                     PQFITEEDPNLRKATAIVNGTVITRTEVDHRLAIIRMAAGGQIPAEELPRLRQQVLRN
                     LVDEVLQIQAAEQADIKITKGEIDQYFARYAQGQFKMTPDQMAQYLRS"
     gene            complement(521..2749)
                     /gene="lptD"
                     /locus_tag="VGB57_08195"
     CDS             complement(521..2749)
                     /gene="lptD"
                     /locus_tag="VGB57_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY0131372.1"
                     /translation="MKKLNLICLTALPLLVAAPGAAFAQETGPVIDEGTSAPAADSRE
                     VAFSSDELSYDSEAQVVTASGDVRMNSEGNRLRAERVTWNRATDEVRAEGNVRVVNPG
                     GDVAYADSVVLTDALRDGIVQNMLLVLADGGRLAADRAERKDGYTTLYRAAYSPCAVV
                     NEKGCPKNPTWQINAVRVVHDPVKGRIRYEGASLNLLGARIAALPFLSHPDGSEGGGS
                     GVLVPDIRLSSSNGFELAVPYYLKLAPNRDATITPHVYTEVAPMLEGRYRQLTGLGAF
                     QVGAYVTYGSRIPITVPLTTLKDEGVRAYLEGNGRFQLGPLWTVTASGRYVTDRTFLR
                     RYDISRDTRLRSVIEAERITSDSYISIAGWAFQGLRVTDVAGQQPIALPAIDARWRLP
                     GKVWGGRVEVQANSLAILRTSGQDTQRAFAGARWDRRSLTPWGQEMLLTAYARGDLYH
                     SSDNLLTDTIIYRGEEGWHARAIGALAAEFRWPLVGPFMGGTQRLTPRLQLVASGATD
                     NLEVPNEDARAVDLEDSNLFALNRFPGHDRWENGPRVTYGVDWGLDLPGIAVRTTIGQ
                     SYRLDDKPSILPPGTGLSDRFSDYVGRTNVKVGRKLNLVHRFRIDKESLALRRNEVDA
                     VVGGRQTYATIGYLRLDRNIDPSIEDLRDREEIRLGGRVRFARYWSIFGSTVVDLTDA
                     KESPLSSADGYEPVRHRIGVSYDDDCLEIGVTWRRDYDRVGDIDRGNTFQFRVALKNL
                     GR"
     gene            3010..4488
                     /locus_tag="VGB57_08200"
     CDS             3010..4488
                     /locus_tag="VGB57_08200"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0131373.1"
                     /translation="MKISFASERPAGAYALALPVWSEDMVADRLGGFDPSARAVAARA
                     AEAQRFEREAASVAETFVAEGEGARRLLLIGLGDRRQDDALFEKVGGALTARLLTSGE
                     TRLVVDLTGLGLGGDAAARIGLGAAARSWRFDQYRTKLTKKQKPTLEEVVIVGGGDSA
                     SAWNDRAALLDGLSFTRELVTEPANILYPESFVERCRKRLEPLGVEFEVLGEDQMRAL
                     GMGALLGVAQGSVRPPRLLAMRWNGASDARARPVVLIGKGITFDTGGISIKQAAGMEA
                     MKWDMGGAGAVAGAMAALAGRKAKANVVAVCALAENMPDGNAQRPGDVVTSMSGQTIE
                     VINTDAEGRLVLCDAMTWAQRQYRPEVIVDLATLTGAMIISLGHEYAGLFSNDDGLAG
                     QLTAAGAASGDKLWRMPLGDPYDKLIDSPIADMKNVGPREGGSITAAVFLQRFVETGV
                     KWAHLDIAGMAWADKASHLYDKGATGYGVALLDRFIAANYEG"
BASE COUNT          700 a         1515 c         1584 g          741 t
ORIGIN      
        1 cgaccgcaga tattgcgcca tctggtcggg ggtcatcttg aactggccct gggcgtagcg
       61 cgcgaaatac tgatcgatct cgcccttcgt gatcttgatg tcggcctgct cggcggcctg
      121 gatctggagg acttcgtcga cgaggttgcg aagcacctgc tggcggagtc ggggaagctc
      181 ctccgccgga atctggccgc ccgccgccat cctgatgatc gcgagccggt gatcgacctc
      241 ggtccgcgtg atgacggtgc cgttgacgat cgccgtcgcc ttgcggaggt tgggatcttc
      301 ctcggtgatg aattgcgggt tggccgggat gttgagtctc gccgacggcg ccgccgggcc
      361 gggatcgctc accgtctgcg ccacggccgc cgcggcggcc agtgagccgg ccagcatgag
      421 cgccgccccg tgataaatgc cgagcttcat cattcctcca attaatgcag gccgccagcg
      481 atgcctgaaa tttgctgaac cccagcttga ccgggcatct ttagcgtccg agattcttca
      541 acgccacccg gaactggaag gtgttgccgc ggtcgatgtc gccgaccctg tcgtaatcgc
      601 ggcgccaggt cactccgatc tcgaggcaat cgtcgtcata gctgaccccg atccggtggc
      661 gaaccggctc atagccgtcg gccgaggaca gcgggctctc cttggcgtcg gtgaggtcga
      721 cgacggtcga gccgaagatc gaccagtagc gcgcgaagcg cacccggccg ccgagccgta
      781 tctcctcgcg gtcgcgcaga tcctcgatcg aggggtcgat attgcggtcg aggcggaggt
      841 agccgatcgt cgcataggtc tggcgccccc cgaccacggc atcgacctcg ttgcgcctca
      901 gcgccaggct ttccttgtcg atccggaagc ggtggacgag gttgagcttg cggccgacct
      961 tgacgttggt cctgccgaca taatcggaga agcggtcgga aagtccggtc ccgggaggaa
     1021 ggatgctcgg cttgtcgtcc aggcgatagc tctggccgat cgtcgtccgg accgcgattc
     1081 ccggaagatc gaggccccag tcgactccgt aggtgacccg cgggccgttc tcccagcggt
     1141 cgtggccggg gaagcggttg agggcgaaga ggttggaatc ctccagatcc accgcccgtg
     1201 cgtcttcgtt cgggacctcc agattgtcgg tcgcgcccga cgcgaccagc tggaggcggg
     1261 gagtgaggcg ctgggtgccg cccatgaacg gcccgaccag aggccagcga aactcggccg
     1321 cgagcgctcc gatcgcccgg gcatgccagc cttcctcgcc gcgatagatg atcgtgtcgg
     1381 tgagcagatt gtcgctgctg tggtagaggt cgccgcgcgc ataagcggtt agaagcatct
     1441 cctggcccca gggcgtgagg ctgcgccggt cccagcgcgc tccggcgaag gcgcgctggg
     1501 tgtcctggcc gctggtccgg aggatcgcga ggctgttcgc ctgcacctcc acccggccgc
     1561 cccagacctt gcccggcaac cgccagcgcg cgtcgatcgc gggcagggcg atcggttgct
     1621 gcccggcgac gtcggtgacc cgaaggccct ggaaggccca gcccgcgatc gagatgtagc
     1681 tgtcggaggt gatccgctcg gcctcgatca ccgagcgcag gcgggtgtcg cgcgatatgt
     1741 cgtagcggcg aaggaaggtg cggtcggtga cgtagcggcc cgacgcggtc acggtccaca
     1801 atggcccgag ctggaagcgg ccgttcccct cgagataggc gcgcacgcct tcgtccttga
     1861 gggtggtgag cggcacggtg atcggaattc gggacccgta ggtcacgtag gcgccgacct
     1921 gaaaggcgcc gagcccggtc agctggcggt agcggccctc cagcatcggc gccacctcgg
     1981 tgtagacgtg gggcgtgatc gtggcgtcgc ggttgggcgc cagcttcaga taatagggaa
     2041 ccgccagctc gaagccgttg ctcgagctca gccttatgtc gggcacgagc actccgcttc
     2101 cacccccctc gcttccgtcc gggtgcgaga ggaagggcaa ggcggcgatt cgggcgccga
     2161 gcaggttgag cgacgcgcct tcgtagcgga tccggccctt gacgggatcg tggacgaccc
     2221 ggacggcgtt gatctgccag gtcgggttct tcgggcagcc tttctcattc acgaccgcgc
     2281 agggcgagta ggcggcgcga tagagggtcg tgtagccatc cttgcgctcg gccctgtcgg
     2341 cggcgaggcg gccgccgtcg gcgagcacca ggagcatgtt ctggacgatt ccgtcgcgaa
     2401 gggcgtcggt cagcacgacg ctgtcggcat aagcgacgtc tccgcccgga ttgacgaccc
     2461 ggacgttgcc ctcggcgcgg acctcgtcgg tggcccggtt ccaggtcacc cgctcggcgc
     2521 gcagccggtt gccctcgctg ttcattcgga cgtcgccgct cgcggtcacg acctgagcct
     2581 cgctgtcgta gctgagctcg tcggagctga acgccacctc ccgcgaatcc gcggcgggcg
     2641 ccgaggtgcc ctcgtcgatg accgggccgg tctcctgcgc gaaggcggcg ccgggggcgg
     2701 cgaccagcag cggaagcgcg gtcaggcaga tgagattgag cttcttcact ttccccgccg
     2761 ccagagcacg cgcgaccctt tcgcgcctat cgcaccggaa cggcaaggcc gcaatgccgc
     2821 agggagaggc gggtggccgg gtataaccgt catcccggcg aaagccggga cctcatggcg
     2881 aaaaaggcgc gtttccgtcc tgaagtcccg gctttcgccg ggatgacgaa cggtcggctc
     2941 gccccccttt gcagcggcgg ggctaacgcc tagaggcctg aagtcaaagc caggagcaaa
     3001 gtgccgccca tgaagatcag cttcgccagt gaacgtcccg ccggcgccta tgcgctcgcc
     3061 cttccggtgt ggagcgagga catggtcgcc gatcgtctcg gcggcttcga tccctccgcc
     3121 cgtgccgtcg ccgcccgggc cgccgaggcc caacgcttcg agcgcgaggc cgcttcggtc
     3181 gcggagacct tcgtcgccga aggggagggg gcccggcgcc tgcttctgat cgggcttggc
     3241 gaccgccgtc aggacgacgc cctgttcgag aaagtcggcg gagcgctgac ggcgcggctc
     3301 ctgacctcgg gcgagacccg cctcgtcgtc gatcttaccg gcctcggcct cggcggcgac
     3361 gccgcggccc ggatcggcct cggcgcggct gcccgaagct ggcgcttcga tcaataccgg
     3421 accaagctca ccaagaagca gaagccgacc ctggaggaag tggtcatcgt cggcggcggc
     3481 gattccgcct ccgcctggaa cgaccgcgcc gccctgctcg acggtctctc cttcacccgc
     3541 gagctggtca ccgagcctgc caacatcctc taccccgaaa gcttcgtcga gcgctgccgc
     3601 aagcggctcg agcctctcgg ggtcgaattc gaggtgctgg gcgaagacca gatgcgggcg
     3661 ctcggaatgg gcgcgctgct cggagtggcc cagggctcgg tgcggccccc gcggctgctc
     3721 gctatgcgct ggaacggcgc ctccgacgcc cgcgcccggc cggtcgtcct gatcggcaag
     3781 ggcatcacct tcgacaccgg cggcatctcg atcaagcagg ctgcgggcat ggaggcgatg
     3841 aagtgggaca tgggcggcgc cggcgccgtc gccggcgcga tggcggcgct cgccggccgc
     3901 aaggccaagg cgaatgtggt cgcggtctgc gcgctcgccg agaacatgcc cgacggcaac
     3961 gcccagcgtc ccggcgacgt cgtcacgagc atgtcgggcc agacgatcga ggtgatcaac
     4021 accgacgccg agggccggct ggtcctgtgc gacgcgatga cctgggccca gcgccagtac
     4081 aggccggagg ttatcgtcga tctcgccacc ctcaccggag cgatgatcat ctctctcggc
     4141 cacgaatatg ccggcctgtt ctcgaacgac gacggccttg ccggccagct gacggcggcg
     4201 ggcgcggcga gcggcgacaa attgtggcgg atgccgctcg gcgatcccta cgacaagctg
     4261 atcgattcgc cgatcgccga catgaagaac gtcggcccgc gggaaggcgg ctcgatcacg
     4321 gcggcggtct tcctccagcg cttcgtcgag accggggtga aatgggccca tctcgacatt
     4381 gccggaatgg cctgggcgga caaggcgagc catctctacg acaagggcgc gaccggctac
     4441 ggagtcgcct tgctcgaccg cttcatcgcg gcgaattacg aaggatgatc cgtcatcccg
     4501 gcgcaagccg ggacctcggg gagacccgcc gtgcgttctt
//
LOCUS       DASWNU010000417         4356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_509120, whole genome shotgun sequence.
ACCESSION   DASWNU010000417 DASWNU010000000
VERSION     DASWNU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509120"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="egtD"
                     /locus_tag="VGB57_08205"
     CDS             <1..831
                     /gene="egtD"
                     /locus_tag="VGB57_08205"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY0131374.1"
                     /translation="ITGLPEYYLPRTETALLERRCPDVARIAGPGQAIVEFGSGASVK
                     TRILLAAAAPSAYVPIDISGDFLRDAAASLADDFPGLPVHPIEADFTRPIRLPEALAA
                     LPKLGFFPGSTIGNMIPATGVDLLRAMRLTLGTGAWLFIGFDRIKDPAELLRAYDDEA
                     GVTAEFNLNLLERINRELGGDIPVESFRHRAVWNDRLSRIEMHLQAARDIGFEVAGRH
                     FTMSAGETIHTENSHKYGERDARLLLLAGGWTPVEEWTDEGARFALVLARAEPERSAP
                     "
     gene            complement(915..1904)
                     /locus_tag="VGB57_08210"
     CDS             complement(915..1904)
                     /locus_tag="VGB57_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEY0131375.1"
                     /translation="MITMLLLAAAAPQPAQLKTFQDWTVGCDNGRACHAVALMPEDWR
                     DDALTMSVRRGPEAGAQPVVAFELGSDSNAASVSADGRRLAARLVGAEGETRVAPADT
                     AAVLAALRSAGQLRLHGADGKPLGMVSLKGASAALLYMDEQQRRIGTPTALVRRGTRA
                     AAAAVPPLPVVAAPPVSRARPLALSAAALAALRKKHGCTLDEVGGPEEAETAALGGGE
                     TLLLLACGSGAYNVSYVPFVVRRGRAELAAFDHRPGWWAEEGKPMLTNAGWDSKRGLL
                     SSFSKGRGLADCGTDSDYAWDGRAFRLVKQAEMDECRGSRDYITTWRARLVRR"
     gene            complement(1901..3169)
                     /locus_tag="VGB57_08215"
     CDS             complement(1901..3169)
                     /locus_tag="VGB57_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131376.1"
                     /translation="MGLISRRHWKKLVAQAGHVLDPLFKSILAFMLTLALSMGVISAL
                     SGIVAVATDYHPNYLFAYFLEVGILFIFLLATIAAVAIYFLGLWQNRHRIGRIALYIL
                     LGLGGLIIPTSAALLHQEHYLAAPQDYVLPAERSPYFKAVSRRLDRTALAEIERLTAV
                     RRRVAKLTAESGSSGVMLRFLDGVPSRRNCSRLDLGTDSGAICVRSYVEAAGKGNIIE
                     RFEVWADAGLDARPNTLDLVPLVSGTRFFCPMAEVFKESSGKVRAEIPVADFARCLGA
                     AEKAIGARIADAQAAMAHRKKYPAIPFSALFVDNAAALLGARYTVLEPVSPRAGLISV
                     AQSLLALAYFVLFASYVIETASKKLPKRAMIGTLGLNGGIMRSGLDGLSRPLAHVSRA
                     PILRRRWGLRVKPGKKTRWRRGRRKVSVPK"
     gene            3417..3719
                     /locus_tag="VGB57_08220"
     CDS             3417..3719
                     /locus_tag="VGB57_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0131377.1"
                     /translation="MLLALIQILLILLTVVWWIIVIQMILSWLVAFNVINTHNDFVRG
                     LMRGLDRFLEPVYRPIRKVLPDFGGLDFSPMVVLIVLLILDRLLRGVALDVAMSYR"
     gene            3716..>4356
                     /locus_tag="VGB57_08225"
     CDS             3716..>4356
                     /locus_tag="VGB57_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEY0131378.1"
                     /translation="MSAARIDGRAFAARLRERVARAVPAFAAAAGRAPGLAVVLVGDD
                     PASQVYVRSKGRATEEAGMAGFEHRLPDGVSQEALLALVDQLNADEAVDGILVQLPLP
                     AGIDDKAVIEAIDPAKDVDGFHPINAGRLAVGEEGLVPCTPLGCLMLLKDRLGDLSGL
                     EAVVIGRSNIVGKPMAQLLLMENCTVTVAHSRTRGLADVVRRADILVAAVGRA"
BASE COUNT          736 a         1507 c         1394 g          719 t
ORIGIN      
        1 atcaccgggc ttcccgaata ttatctcccc cgaaccgaaa ccgccctgct cgagcggcgc
       61 tgcccggacg tcgcgcggat cgccggcccc ggccaggcga tagtcgagtt cggctccggg
      121 gcctctgtca agacccggat ccttctcgcc gcggccgctc cgtccgctta tgtgccgatc
      181 gacataagcg gcgacttcct gcgcgacgcg gccgcctcgc tggccgacga ctttccgggc
      241 ttgcccgtcc acccgatcga ggcggatttc acccgcccga tccggcttcc cgaggcgttg
      301 gccgctctgc cgaagctcgg cttcttcccc ggctcgacca tcggcaacat gatccccgcg
      361 accggagtcg acctgctcag ggcgatgcgc ctgacgctcg gcaccggcgc ctggctgttt
      421 atcggcttcg accggatcaa ggatccggcc gagctgctgc gcgcctatga cgacgaggcc
      481 ggagtgacgg ccgagttcaa cctcaacctc ctcgagcgga tcaaccgtga gctgggcggc
      541 gacatcccgg tcgaatcctt ccgccaccgg gccgtctgga acgacaggct gagccggatc
      601 gagatgcatc tgcaggccgc gcgcgacatc gggttcgagg tcgccggccg gcacttcacg
      661 atgagcgcgg gcgagaccat ccacaccgaa aatagccaca aatatggcga acgcgacgcg
      721 aggctgctgc ttctcgcggg cggctggacc ccggtggagg agtggaccga cgagggggcg
      781 aggttcgcgc tggtgctggc ccgcgcggag ccggaacggt cggcgccctg atcctgtcct
      841 gtcatcccag cgggtgaaag gcgcctgcgc tcttcacccg ccggggcccc agcttcgctg
      901 gggcgacggc taccctacct gcgtaccagc cgtgcccgcc aggtcgtgat gtaatcgcgc
      961 gagccccggc actcgtccat ctcggcctgc ttcaccaggc ggaaggcgcg gccgtcccat
     1021 gcgtaatccg agtcggtgcc gcaatcggcg agcccgcggc ccttggagaa gctggacaga
     1081 aggccgcgtt tcgaatccca accggcattg gtgagcatcg gcttgccttc ctccgcccac
     1141 cagcctggcc ggtggtcgaa ggcggcgagc tcggcacggc cgcgcctcac gacgaaggga
     1201 acgtagctga cattataggc gcccgagccg caggcgagca gaagcaaggt ctcgccgccg
     1261 cccaaagcgg cggtctccgc ctcctccggg ccgccgacct cgtcgagcgt gcagccatgc
     1321 ttctttcgca gtgccgccag cgccgccgcg ctcaaggcca gcggcctcgc ccgtgaaacc
     1381 ggcggcgccg ccacgaccgg aagcggcggc accgcggcgg cggccgctcg cgtgccgcgc
     1441 cggacgaggg ccgtcggcgt gccgatccgg cgctgctgct cgtccatgta aagcaaggcg
     1501 gcgctcgcgc ccttgagcga gaccatcccc aggggcttgc cgtcggctcc gtgcaatcgc
     1561 agctgcccgg ccgagcgcag ggcggccaac accgccgcgg tgtcggccgg agcgacccgg
     1621 gtctcaccct cggccccgac cagacgcgcc gcgagcctcc tgccgtccgc gctcaccgac
     1681 gcggcgttgc tatccgagcc gagctcgaag gcgacgaccg gctgagcccc ggcttcgggc
     1741 ccgcgcctta cgctcatcgt cagggcgtcg tcgcgccaat cctcgggcat cagcgccacc
     1801 gcgtggcagg cgcggccgtt gtcgcatccg acggtccagt cctggaaggt cttgagctgg
     1861 gctggctgcg gggcggcggc ggcgaggagg agcatggtga tcatttcggc acgctaacct
     1921 tccggcggcc acggcgccag cgcgtcttct ttccgggttt cacgcgcaag ccccatcgac
     1981 ggcgcaaaat cggcgctctc gatacgtgag ccagcggccg cgataggccg tcgagccccg
     2041 accgcataat cccgccgttc agaccaaggg ttccaatcat cgcgcgcttc ggaagcttct
     2101 tggaggccgt ttcgatcacg taggaggcga aaagaacgaa ataagcgagc gccagcaacg
     2161 actgggcaac gctgatcagc ccggctctcg gcgaaaccgg ctccaggact gtatatcgcg
     2221 cacccagcag cgccgcggca ttgtcgacga acagggccga gaacggaatt gcgggatatt
     2281 tcttgcggtg tgccatggcg gcctgcgcgt ccgcgatcct ggcgccgatc gccttctcgg
     2341 ccgccccgag acagcgagcg aaatccgcga ccggaatttc agcccgcacc ttgcccgacg
     2401 actccttgaa cacctccgcc attggacaga aaaaccgggt gcccgaaacc aatggaacca
     2461 gatcaagggt attcggacgt gcatcgaggc cggcatccgc ccaaacttcg aagcgctcta
     2521 ttatatttcc tttcccagcg gcctccacat aggaccgtac gcaaatggcg ccgctatcgg
     2581 tgccgagatc gagtctcgag caattgcgcc tcgaggggac gccgtccagg aagcgcaaca
     2641 tcactcctga cgaacccgac tccgcggtca gcttcgccac ccttcgccgg acggcggtca
     2701 gccgttcgat ttccgccaaa gcggtccggt caagccttcg gctcacagcc ttgaaatagg
     2761 gcgaccgctc cgccggcagg acatagtcct gcggagcggc gagatagtgc tcctgatgca
     2821 acagggctgc gctcgtcggg atgatcaggc ctccgagacc gagtaagatg tagagtgcga
     2881 ttcgaccgat cctgtggcgg ttttgccaaa gacctaaaaa ataaattgct actgctgcta
     2941 tggttgcaag taaaaatatg aacaaaattc cgacttctag aaaatatgcg aaaagataat
     3001 taggatgata atcagtggcg acggcaacta tgccgctcag tgcactaatg actcccatag
     3061 atagcgccag tgtaagcatg aacgcgagaa tgctcttgaa cagtggatcg aggacgtgcc
     3121 ccgcctgtgc cacaagcttc ttccagtgac gtcgtgatat cagccccatc gtccggttcc
     3181 tccagcatgg cgcagtgtga catgtcatca ccggggagcg aagcctggcc gtccttggcg
     3241 atctcctcgg cgagccgatc cacgagggac ggggagcatc cggcaactgg aatccaggcg
     3301 ccccgttcga cagaggatcg cggataggac aaggggggtc ccctgccgga tcccagccat
     3361 tgcccccgcc gcgccgtctc cctacttagc cgggagcgac ccccggggag agaatgatgc
     3421 tgctggccct gatccagata ttgctgatcc tgctgacggt ggtctggtgg atcatcgtca
     3481 tccagatgat cctgagctgg ctggtcgcct tcaacgtcat caacacccat aacgatttcg
     3541 tgcgtgggct gatgcgcggc ctcgaccgct tcctggagcc tgtctaccgg ccgatccgca
     3601 aggtgctgcc cgatttcggc gggctcgatt tcagcccgat ggtcgtcctc atcgtcctcc
     3661 tcatcctcga ccggctactg cgcggagtcg ctctcgacgt tgcgatgagc taccggtgag
     3721 cgcggcgcgc atcgacggcc gcgccttcgc cgcgcggctg cgcgagcgcg tcgcccgggc
     3781 ggtccccgcc ttcgccgccg ccgccggccg ggctcccggc ctggccgtgg tcctggtcgg
     3841 ggacgatccg gcgagccagg tctatgtccg ttccaagggt cgggcgaccg aagaggcggg
     3901 catggccggc ttcgagcatc gcctgcccga cggcgtctcg caggaggcgc ttctggcgct
     3961 ggtcgaccag ctcaacgccg acgaggcggt cgacggaatc ctcgtccagc tgccgcttcc
     4021 ggccgggatc gacgacaagg cggtgatcga ggcgatcgac cccgccaagg acgtcgacgg
     4081 cttccatccg atcaatgccg ggaggctcgc ggtcggcgag gaggggctgg tcccctgcac
     4141 tccgctcggc tgcctgatgc tcctcaagga ccggctcggc gacctttcgg gtctcgaggc
     4201 ggtggtgatc ggccgctcca acatcgtcgg caagcccatg gcgcaattgc tgctgatgga
     4261 gaattgcacg gtcaccgtcg cccacagccg cacccgcggc ctcgccgacg tcgtgcgccg
     4321 tgccgacatc ctggtcgccg cggtggggcg tgcgga
//
LOCUS       DASWNU010000418         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_379256, whole genome shotgun sequence.
ACCESSION   DASWNU010000418 DASWNU010000000
VERSION     DASWNU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379256"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..984
                     /locus_tag="VGB57_08230"
     CDS             40..984
                     /locus_tag="VGB57_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0131379.1"
                     /translation="MTVYLPTLKQLQYLVTLQDTGHFGKAAESCFVTQSTLSASLREL
                     ESLIGVTLVERNRRVVGFTPLGARIAEKARRVLREAEELADMARAAGQPLTGDLRMGV
                     IPTIAPFLLPTMLPVLRREWPKLKLYLREETSQSACEALHRGQLDCVLLALPYACGDV
                     ASVPLFDDPLFVAFPRGEAPGGAEVEVAAIDENRLLLLEDGHCLKDHALSACNRPDLR
                     AHAAMMGTSLHTLVQMVDNGLGVTFIPSMAIEAGILEGTRVDARPLRSDHGFRRIALI
                     WRRSSPRESEFQLLASALSRIIGELSPGREASGPAAPP"
     gene            complement(941..1228)
                     /locus_tag="VGB57_08235"
     CDS             complement(941..1228)
                     /locus_tag="VGB57_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131380.1"
                     /translation="MQRHEVDSLDPARTYWVPSVIAPQRNWSGAPGCRKGARFLVDGR
                     TFRPSREEFQPFDSEFACLGWIMRHRAQLNRNLCGVRIKAVPLDRWLLGLD"
     gene            complement(1348..>1970)
                     /locus_tag="VGB57_08240"
     CDS             complement(1348..>1970)
                     /locus_tag="VGB57_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY0131381.1"
                     /translation="DAGFDIEVPFTGGRGDASQDWTDAESFAVMEPAADGFRNYLKTK
                     HSVRTEELLLDRASLLGLSVPEMTVLLGGLRVLGANHQDRPEGVFTTRKGQLTNDFFV
                     NLLDNDTFWQVVDESGDEEFIGYDRGAMKENGQEKWRATRTDLIFGANSQLRAVAEVY
                     AEKGHEEKFVHDFISAWTKVMNADRFDLPQNPDSVEPIGIAEPAAG"
BASE COUNT          299 a          688 c          642 g          341 t
ORIGIN      
        1 tcaaggcatt tagaacgatc aaattgatcg ggaaaagcga tgacagtcta tctgcccacc
       61 ctcaagcagc tccagtatct cgtgacgctc caggacaccg gccatttcgg caaggcggcg
      121 gagagctgct tcgtcaccca gtcgaccctg tccgccagcc tgcgcgagct cgaatccctg
      181 atcggggtca ccctggtcga gcgcaaccgc cgggtcgtcg gcttcacccc gctcggcgcc
      241 cggatcgcgg agaaggcgcg gcgagtcctt cgcgaggccg aggagctggc cgacatggcg
      301 cgggcggcgg gccagccgct caccggggat ctgaggatgg gagtcatccc gaccatcgcc
      361 cccttcctcc tgccgacgat gcttccggtg ctccgccgcg aatggcccaa gctcaagctc
      421 taccttcgcg aggagaccag ccagtccgcc tgcgaggcgc ttcaccgggg ccagctcgac
      481 tgcgtccttc tggcgttgcc ctacgcctgc ggcgacgtcg cttcggtccc gctgttcgac
      541 gatccgctct tcgtcgcctt tccccgcggc gaggcacccg gcggggctga ggtcgaggtg
      601 gcggcgatcg acgagaaccg gcttctgctc ctggaggacg gccactgcct caaggaccat
      661 gcgctgtcgg cctgcaaccg gcccgacctg cgggcgcatg cggcgatgat gggcacgtcg
      721 ctccacaccc tcgtccagat ggtcgacaac gggctcggag tgaccttcat cccatcgatg
      781 gcgatcgagg ccgggatact cgagggcact cgggtcgacg ccaggccgct ccgctccgac
      841 cacggcttcc gccgcatcgc cctgatctgg cgccgctcaa gcccgcgcga aagcgagttc
      901 cagctgctcg cctcggcgct gagccggatc atcggcgagc tcagtccagg ccgagaagcc
      961 agcggtccag cggcaccgcc ttgatcctca ctccgcacag atttcggttg agctgcgcgc
     1021 ggtggcgcat gatccagccc aggcaggcga actcgctgtc gaacggttgg aattcctccc
     1081 ggctcggccg gaacgtccgg ccgtcgacca ggaagcgggc gcccttgcgg caaccggggg
     1141 cgccggacca gttccgttga ggcgcgatga cggacggcac ccaataggtg cgggctggat
     1201 ccaggctatc gacttcgtgt cgctgcatcg ggcctccgcc ttcctcccgc aaccgtcatt
     1261 gcgagcgtag cgaagcaatc cagcggcgca agcgtcgggg actggattgc ttcgccgctt
     1321 cgctcctcgc aatgacggcc cggaccccta tccggccgcc ggctcggcga ttccgatcgg
     1381 ctcgacgctg tccggattct gcggcaggtc gaagcgatcc gcgttcatca ccttggtcca
     1441 ggcggagatg aagtcgtgca cgaacttctc ctcgtggccc ttctcggcat agacctcggc
     1501 gacggcgcgg agctggctgt tggcgccgaa gatgaggtcg gtgcgggtcg ccctccactt
     1561 ctcctgccca ttctccttca tggcgccgcg gtcgtagccg atgaactcct cgtcgccgct
     1621 ctcgtcgacc acctgccaga aggtgtcgtt gtcgagcagg ttgacgaaga agtcgttggt
     1681 gagctggccc ttgcgggtgg tgaagacgcc ctccggtctg tcctggtggt tggcgccgag
     1741 cacgcgcagg ccgccgagga gcaccgtcat ctcgggcacg gacaagccca gcagcgaggc
     1801 tcggtcgagc agcagctcct cggtccggac gctgtgcttg gtcttcaaat agttgcggaa
     1861 gccgtccgcc gccggctcca tgacggcgaa gctctcggca tcggtccagt cctggctggc
     1921 gtcgccgcgg ccgccggtga acggcacctc gatgtcgaag ccggcgtcgc
//
LOCUS       DASWNU010000419         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_170515, whole genome shotgun sequence.
ACCESSION   DASWNU010000419 DASWNU010000000
VERSION     DASWNU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170515"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VGB57_08245"
     CDS             complement(<1..927)
                     /locus_tag="VGB57_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEY0131382.1"
                     /translation="MTHYTPIMAAAVRAADLSDAAERGRIDAYVAEHPESVLFHRPQW
                     SQAVERGCGQRSHYLVAERPGGVLVGCLPLTDVRSALFGRALVSTGFGTGGGVLADDA
                     PAAGALLGAAGELADRIGCATLELRGGEFPGDYAVRDDVYVGFAMDLPQGEAAIFKSI
                     KRRHRGVRRARERGLGVRVGAAPRDRSDFFLVYGESMRNLGSPVFPPRLFEAMLDLFG
                     EDADILTISEGDSPVASVLNFYFKGIVHPYWGGGTTAARDCFASELMYFEVMCHAARR
                     GCTRFDFGRSKVGTGNYSFKMNWGMDPEPLRYG"
     gene            complement(924..1769)
                     /locus_tag="VGB57_08250"
     CDS             complement(924..1769)
                     /locus_tag="VGB57_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HEY0131383.1"
                     /translation="MLNALSVDVEDWFQVGAFEAVIRRDQWDGLEQRVERNAEAVLDL
                     FAEARVKATFFTLGWVAERHPALIRRAAAQGHEIASHGWDHQRVFNLGEEGFRADLRR
                     AHDAISKAAGVEPKGYRAPSFSIDARTPWAHRILAEEGYAYSSSVAPIAHDHYGWREA
                     PRFAHRPLADRDLIELPVTTVELAGRRMAAGGGGFFRLLPYRFSHWAIDRVNKEDRRP
                     AVFYFHPWEIDPGQPRVAGAPLKSRLRHYSRLSAMRPKLLKLFKAHTWGRTDAVVEGE
                     RERLS"
     gene            complement(1923..2939)
                     /locus_tag="VGB57_08255"
     CDS             complement(1923..2939)
                     /locus_tag="VGB57_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0131384.1"
                     /translation="MYTDHYGLSERPFQLTPDARYWYESRTHKKAMAYLGYGLAQGEG
                     FIVVTGEIGAGKSTLVAHLMATIDRERLNAISLVSTQVEGDDMLRLAAQGLGLETGGV
                     AKAQLLDRIEHRLREEAGRGKRTLLVVDEAQNLPVSALEELRMLSNFTSQGRALIQIF
                     LLGQPEFRDRVADEDSLEQLRQRVIASHHLEAMEANEVEPYAVHRLAVAGWQGRPGFE
                     PDAFAALYRHTGGVPRKLNQLATRLLLYAGVERLETIGGNDVEAVAADMAADRPASAS
                     KRVLPLRPPSATAEPAADASLDRRIAAIEARLDEQEAAVRRVLTVLLDWVENESQPRR
                     SNAA"
     gene            complement(2953..>3453)
                     /locus_tag="VGB57_08260"
     CDS             complement(2953..>3453)
                     /locus_tag="VGB57_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131385.1"
                     /translation="PSDGSLTGNGCVFGNDPGTGRCFGDAFQSIANFNFRNRGANVQY
                     SAVRGPWTMGLGAGYANRKYLAPDSSIFALNGVTDQSFSLQGNLGRRLTRSSGYDIDA
                     YAAWFDSGIADSDASFGAGLTGRYYRNVFRDRLQANIAAGLYTTQAGEFDASYGSILV
                     GLRYTF"
BASE COUNT          564 a         1117 c         1224 g          548 t
ORIGIN      
        1 gccgtagcgc aggggctccg gatccatgcc ccagttcatc ttgaagctgt aattgccggt
       61 gccgaccttg gaacggccga aatcgaagcg ggtgcagccc cgccgggcgg cgtggcacat
      121 cacctcgaaa tacatgagct cgctggcgaa gcagtcccgc gccgccgtcg ttccgccgcc
      181 ccaataggga tggacgatgc ccttgaagta gaaattgagc acgctcgcga ccgggctgtc
      241 gccttcggag atggtgagga tgtccgcatc ctcgccgaac aggtcgagca tcgcttcgaa
      301 caggcggggc gggaagacgg gcgagccgag gttgcgcatc gattcgccgt agacgaggaa
      361 gaaatcggac cggtcccggg gggccgcgcc cactcggact ccgagcccgc gctcccgcgc
      421 ccgcctgact ccgcggtggc ggcgcttgat cgatttgaag atcgccgcct cgccctgcgg
      481 caaatccatg gcgaagccga catagacgtc gtcccggacc gcataatcgc ccggaaactc
      541 tcctccgcga agctcgaggg tggcgcagcc gatccggtcg gcgagctctc cggcggcgcc
      601 cagtagcgct ccggcggcgg gcgcatcgtc ggcgaggacc ccgccaccgg tgccgaagcc
      661 ggtcgagacc agggcccggc cgaacagcgc cgaacggacg tcggtgagcg gaaggcagcc
      721 gacgaggacg ccgccgggac gttcggcgac cagataatgg cttcgctggc cgcagccccg
      781 ctcgaccgcc tggctccact gcggacggtg gaaaaggacg gattccggat gctcggcgac
      841 ataggcgtcg atccggccgc gctcggcggc atcggacaaa tcggcggcgc gcaccgcggc
      901 cgccatgatc ggagtgtagt gggtcacgac agcctctccc gctcgccctc gacgacggcg
      961 tcggttcggc cccaggtatg agccttgaac aatttcagaa gcttgggacg cattgccgag
     1021 agacggctgt aatggcgaag ccgggatttg agcggcgctc cggcgacccg gggctggccc
     1081 ggatcgatct cccaggggtg gaaatagaag acggctgggc ggcggtcctc cttgttcacc
     1141 cggtcgatcg cccagtggga gaaacggtaa gggagaagcc ggaagaagcc cccgcctccc
     1201 gccgccatcc gccgtccggc gagctcgacg gtggtcaccg gaagctcgat caggtcgcga
     1261 tccgcgagcg gccggtgggc gaagcgcggc gcttcgcgcc agccataatg gtcgtgggcg
     1321 atcggggcga cgctggagga ataggcatag ccttcctcgg cgaggatccg gtgcgcccag
     1381 ggcgttcggg cgtcgatcga gaagctgggc gcgcggtagc ccttcggctc gacccccgcg
     1441 gctttcgaaa tggcgtcgtg ggcgcggcgc agatcggccc ggaacccctc ctcgccgagg
     1501 ttgaagaccc gctggtggtc ccagccgtgg cttgcgatct catggccttg tgcggcggcg
     1561 cggcggatca gggcggggtg gcgctcggcg acccagccca gggtgaagaa ggtcgccttg
     1621 accctggcct cggcgaacag gtcgagcacg gcctcggcat tgcgctcgac gcgctgctcc
     1681 agcccgtccc actggtcgcg gcggatgacc gcctcgaacg cgccgacctg gaaccagtcc
     1741 tcgacgtcca cggaaagggc gttcagcatc gtattgcctt cccactaaaa tcctccccag
     1801 catttgctgg ggagggggac cgcgcgcagc gcggtggagg agttctaggc gttggaagaa
     1861 cccctccacc atgcttcgca tggtccccct ccccgcgaaa gcgcggggag gattttagaa
     1921 tctcaagcgg cattgcttcg gcgcggctgg ctttcgttct cgacccagtc gaggagcacg
     1981 gtcaagaccc ggcgcaccgc cgcttcctgc tcgtcgaggc gcgcctcgat ggccgcgatc
     2041 cggcggtcca gcgaggcgtc ggccgccggt tcggccgtgg ccgagggcgg gcgcagggga
     2101 aggacccgct tggaggcgct ggccggacgg tcggccgcca tgtccgccgc caccgcctcg
     2161 acatcatttc cgccgatcgt ctcaagccgt tcgactccgg cataaaggag cagtcgggtg
     2221 gcgagctggt tgagcttgcg cggaacgcct ccggtgtgcc ggtagagcgc ggcgaaggcg
     2281 tcgggctcga agcccggccg gccctgccaa ccggccacgg cgaggcgatg gacggcgtag
     2341 ggctcgacct cattggcctc catcgcctcc agatggtggg aggcgatgac ccgctggcga
     2401 agctgctcga ggctgtcctc gtcggcgacg cgatcgcgga actccggctg tcctagcagg
     2461 aaaatctgaa tgagcgcacg gccttggctg gtgaagttcg aaagcatccg aagttcttcg
     2521 agggccgaga ccggaaggtt ctgggcttcg tcgacgacca gcagggtgcg cttgcccctt
     2581 cccgcttcct cgcgaagcct gtgctcgatc cgatcgagca gctgcgcctt ggccactccg
     2641 cccgtctcga gtccgaggcc ctgagcggcg agacgaagca tgtcgtcccc ttcgacctgg
     2701 gtcgagacga ggctgatcgc gttaagccgc tcgcggtcga tggtggccat cagatgggcg
     2761 acgagagtgg acttgccggc gccgatctcg ccggtgacga cgatgaagcc ctcgccctgg
     2821 gcgaggccgt agccgagata ggccatcgcc tttttatggg tgcggctctc ataccagtag
     2881 cgcgcgtccg gcgtcagctg gaaggggcgt tccgaaaggc cgtaatggtc ggtgtacatc
     2941 gcttgacgtc cctcagaaag tatagcgcag gccgaccagg atcgagccgt agctggcgtc
     3001 gaattccccg gcctgggtgg tgtagaggcc ggcggcgatg ttcgcctgga gccggtcccg
     3061 gaagacgttg cggtaatagc gccccgtaag cccggcgccg aagctggcgt cgctatcggc
     3121 gatgccgctg tcgaaccagg cggcataggc gtcgatgtcg tagcccgagc ttcgggtcag
     3181 ccggcggccg agattgccct gcagcgagaa gctctggtcg gtgaccccgt tcagggcgaa
     3241 gatgctgctg tcgggcgcga gatatttgcg gttggcgtag ccggcgccca gccccatcgt
     3301 ccagggtccg cggaccgcgg aatactggac gttggcgccg cggttgcgga agttgaaatt
     3361 ggcgatcgac tgaaaggcgt cgccgaagca gcgtccggtg ccgggatcgt ttccgaacac
     3421 gcagccgttg ccggtgaggc tgccatcgga cgg
//
LOCUS       DASWNU010000420         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_456321, whole genome shotgun sequence.
ACCESSION   DASWNU010000420 DASWNU010000000
VERSION     DASWNU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456321"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="VGB57_08265"
     CDS             <1..1569
                     /locus_tag="VGB57_08265"
                     /inference="COORDINATES: protein motif:HMM:NF017541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HEY0131386.1"
                     /translation="TKEDAMANKGLFASAVARLLAPPADTFNRELSPAYAYRPEHKLA
                     QLAATGTLQDAFYADAETQLAEVLEAAGAVDPLFAARAAVFARSRGAMKDVPALLTAW
                     LTVADPDLAVRIFPRTIDNGRMLRNFVQIMRSGAVGRRSLGSRPKRLVQQWLERASMR
                     SLMQAATGNDPSLADIVRMVHPRPADEARKAFYGWLIGKPWDVAALPAEIAGFEAWKR
                     DPSLPLPEVPFEWLTAFELSAGQWAELASRMGWQALRMNLNTLARKGAFAVEGVTEKV
                     AARLADPAALSKARVLPYQLMVALGSVDEGVPLKVQAALETALEASLANVPAFEGRVV
                     VAPDVSGSMSSPVTGYRKDASSKVRCIDVAALVAAAVLRRNREAMLLPFEVGVVPLKL
                     DPFARVAANAAKLASIGGGGTNVSAPLALLNAQSAQVDLVVIVSDNESWVDSTRHGAT
                     ATMNEWNRLKKRCPAAKLVCIDLQPYGTTQAKSRRDILNVGGFSDAVFETLSRFANGD
                     GERDWVAEVNEMEV"
BASE COUNT          271 a          582 c          606 g          273 t
ORIGIN      
        1 acgaaggaag atgccatggc caacaagggc ctttttgcct ccgcggtggc caggctcctg
       61 gctccgcccg cggacacttt caaccgggag ctttccccgg cttacgctta caggccggag
      121 cacaagctcg cccagctcgc cgcgaccggc accctccagg acgcgttcta cgcggacgcg
      181 gagactcagc tggccgaggt gctggaggcg gccggggcgg tcgacccgct cttcgccgcc
      241 agggccgccg tcttcgcccg ctcgcgcggc gcgatgaagg acgtgccggc cctgctgacg
      301 gcctggctga cggtcgcgga tccggacctc gccgtccgga tcttcccgcg gacgatcgac
      361 aacggacgga tgctgcgcaa cttcgtgcag atcatgcgct ccggagcggt cgggcgcagg
      421 tcgctcggct cccggcccaa aaggctcgtc cagcagtggc tcgagcgggc gtcgatgcgg
      481 tctctgatgc aggccgcgac gggcaacgat ccgtcgctcg ccgacatcgt ccggatggtc
      541 caccccaggc ccgccgacga ggcccgcaag gccttctacg gctggctgat cggcaagccc
      601 tgggacgtcg ccgcccttcc ggccgagatc gccgggttcg aggcgtggaa gcgggacccc
      661 tcgcttcccc tgcccgaagt gccgttcgag tggctgaccg ccttcgagct gtcggccggg
      721 caatgggcgg agctcgcctc ccgcatgggc tggcaggctc tgcgcatgaa cctcaacacg
      781 ctggcgcgca aaggcgcctt cgctgtcgag ggggtgaccg aaaaggtcgc ggctcggctc
      841 gcggatccgg cggcgctgtc gaaggcgcgg gtcctcccgt accagctgat ggtggcgctc
      901 ggatctgtgg acgagggcgt tccgctcaag gtccaggcgg cgctcgagac ggcgctcgaa
      961 gcctcgctcg cgaacgttcc ggcgttcgag ggccgggtag tcgtcgcccc cgacgtatcg
     1021 gggtcgatgt cttcgccggt gacgggctat cgcaaggacg cctcgtcgaa ggtcaggtgc
     1081 atcgatgtcg cggccttggt cgcggcggcg gtgctccgcc gaaaccggga ggcgatgctg
     1141 ctgccgttcg aggtcggggt cgtgccgctg aagctcgatc cgttcgcccg ggtggcagcg
     1201 aacgctgcca aactggcgtc gatcggcggc ggcgggacca acgtctcggc tccgctcgcc
     1261 cttctcaatg cgcagagcgc gcaggtggat ctggtggtga tcgtctcgga caacgaatcc
     1321 tgggtcgatt ccacccgcca cggagccacg gcgacgatga acgagtggaa caggctgaag
     1381 aaaaggtgcc cggcggcgaa gctcgtctgc atcgatctcc agccctacgg gaccacgcag
     1441 gcgaagagcc ggcgcgacat tctcaacgtc gggggcttct ccgacgcggt gttcgagacg
     1501 ctctcccgct tcgccaacgg cgacggggag cgcgactggg tcgccgaagt gaacgaaatg
     1561 gaggtgtaag aaacaaggcc gtggcgaatg ccggcgggac tacatccaca ctcagtgtcg
     1621 ccggttcaaa tccggccggg cccccgggcc cgtagctcag cggttagagc atgagactgt
     1681 cccgcctgaa cctcgtcgcc acggccttca tacgaaagga tgaatcgcgg cg
//
LOCUS       DASWNU010000421         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_249916, whole genome shotgun sequence.
ACCESSION   DASWNU010000421 DASWNU010000000
VERSION     DASWNU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249916"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..>1529
                     /gene="lpdA"
                     /locus_tag="VGB57_08270"
     CDS             163..>1529
                     /gene="lpdA"
                     /locus_tag="VGB57_08270"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0131387.1"
                     /translation="MTDANFDVIVIGAGPGGYVAAIRAAQLGLKTACVESRETLGGTC
                     LNVGCIPSKALLHASELFEEANHGTLAKWGVKVGAVELDLDTMHAGRREAVGGLTKGI
                     EFLFKKNKVTWLKGQGRFTSPTALDVAGTSYTAKNFVIATGSSVTPLPGVEVDQQRIV
                     DSTGALELAEVPKHLVVIGGGVIGLELGSVWRRLGAKVTVVEYLDQILPGMDGEVRKE
                     AAKLFKKQGFDIRTSTRVTGAETTGEGVRLTVEPAKGGEAQTIEADVVLVSIGRRPNT
                     EGLGLEAAGIAPNKGGQIEVDHDFRTAVPGIYAIGDVVPGPMLAHKAEDEGVAVAEIL
                     AGQVGIVNHEVIPSVVYTYPEIAAVGKTEEELKQAGVEIKVGKFPMMANSRAKTNRDA
                     DGFVKVIADARSDRVLGVHIVASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEA
                     LKEAA"
BASE COUNT          287 a          472 c          537 g          233 t
ORIGIN      
        1 gtccccgccg cccgcgatca tctatatccg cttcgaaccg caggacgttg cggacatcat
       61 tccacgcttg ttggccgttc ttgagccgga ggcgttgcag gaccatctga cggtgatcgg
      121 cgatacagcg gatcgccgac gcccgtttcc aaggacagac gaatgactga tgccaatttc
      181 gacgtgatcg tgatcggggc ggggcccggc ggatatgtgg ctgcgatccg ggccgcccag
      241 ctggggctga agacggcttg cgtcgaaagc cgggagacgc tcggcggcac ctgcctcaac
      301 gtcggctgca ttccgtcgaa ggcgctgctg cacgcttccg agctgttcga ggaagcgaac
      361 cacggcacgc tggccaaatg gggggtcaag gtcggcgcgg tcgagctcga tctcgacacc
      421 atgcatgccg ggcggcgcga agcggtcggc gggctgacca agggcatcga attcctgttc
      481 aagaagaaca aggtgacctg gctgaagggg caggggcgat tcacctcgcc gaccgcgctc
      541 gacgttgccg ggacaagcta cacggcgaag aacttcgtca tcgccaccgg ctcttccgtg
      601 acgccgctcc ccggagtgga ggtcgaccag caaaggatcg tcgattcgac gggcgcgctc
      661 gagctggccg aggtgccgaa gcatctcgtc gtgatcggcg gcggcgtgat cgggctcgag
      721 ctgggctcgg tgtggcgccg gctcggggcg aaggtgacgg tggtcgaata tctcgaccag
      781 atcctgcctg gcatggacgg cgaggtccgc aaggaagcgg cgaaattgtt caagaagcag
      841 ggcttcgaca tccgcacgtc gaccagggtc acgggcgccg aaacgactgg cgagggcgtc
      901 cgcctgaccg tcgagccggc gaagggcggc gaggcgcaga cgatcgaggc ggacgtggtt
      961 ttggtctcga tcggccgccg cccgaacacg gagggactcg gcctcgaagc ggccggaatc
     1021 gcgcccaaca aaggtggcca gatcgaggtc gatcacgatt tccgcaccgc ggtccccggc
     1081 atctacgcga tcggcgacgt ggttccgggg ccgatgctcg cccacaaggc cgaggacgaa
     1141 ggcgtcgccg tcgccgagat cctcgccggg caggtcggca tcgtcaatca cgaggtgatc
     1201 ccgagcgtgg tctacaccta tccggagatc gccgcggtcg ggaagaccga ggaggagctc
     1261 aagcaggccg gagtcgagat caaggtcggc aagttcccga tgatggcgaa cagccgcgcc
     1321 aagaccaacc gcgacgccga cggcttcgtc aaggtgatcg ccgacgcgcg ctcggaccgg
     1381 gtgctcggag tccatatcgt cgcctcggtc gccggcacga tgatcgcgca ggccgcgcag
     1441 gcgatggaat tcggagcaac atcggaagat atcgcctaca cctgccacgc gcatccgact
     1501 cattcggagg cgctcaagga ggcggcgat
//
LOCUS       DASWNU010000422         6621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_67690, whole genome shotgun sequence.
ACCESSION   DASWNU010000422 DASWNU010000000
VERSION     DASWNU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67690"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1429
                     /locus_tag="VGB57_08275"
     CDS             26..1429
                     /locus_tag="VGB57_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0131388.1"
                     /translation="MAGEGKPFWRCIVIDDDQDILLSARLLLRDLFEEVLTFQAPEDA
                     MAAVEGDGADVVLLDANFGRGATNAAEGFHWLGEILAKDPQAVVVMITAHGGVNIAVE
                     AMKRGATDFVSKPWSNERLLATVRTAASLRTSRQAAVTERKRAVAISAPAAGGETPLL
                     GQSAPMRRIYSLIERAGPTEANVLILGENGTGKELVARELHGKSRRSGNVFVSVDLGA
                     VAENLFESELFGHVKGAFTDARGDRIGRLQAADGGTLFLDEVGNLPLHLQPKLLTALE
                     QRQVTPVGANKPVSIDVRVVAATNLPPERLADESLFRPDLLFRLNTVEIELPPLRERR
                     EDIPVLVDHYVALYAKKYARPARQLPPEVMAALVAYDWPGNVRALRHAAERAVILAGN
                     GAFTFEDFSLTRTTAPRQAATSTPPALGADLNLERAEKQMVEQALKRNAYNISLAAQE
                     LGLTRASLYRRMDKHGL"
     gene            1582..2922
                     /locus_tag="VGB57_08280"
     CDS             1582..2922
                     /locus_tag="VGB57_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0131389.1"
                     /translation="MGFRSRFVAGLGWRAAMLLGTVYLFVATLGVEGLGVARLLAAGL
                     CLWALYELWRYIQRTNMELARFLEAVRLGDLSQSFAHRADGSGFTDIGQALDQGIRNL
                     RDERLRLTESNRFCEAVLDDAPAPLLTVDTEGRVELANKAARRLFVRHHGVRIEDYRI
                     YGETFATALGDDAIGKPRLVPLMLDELPQTAMVSAASVHRLGGMVRVVAVQPIQGELN
                     AIEIAAQSDLIRVLTHEIMNSMTPVTSLAQSAAGLMVEADRGDDPLVSDARAAVETLA
                     RRADGVMHFVESYRQISRTPVVRRRHFEVLPWARELESLFRAGEGAQGVALSLDVAPD
                     RLTLDADPDLMCQVLINLLRNASEAARDHAEAPAVSLSLAAVAGGRVQIEVADNGAGV
                     PEDLRQDVFLPFFTTKAKGTGVGLSLARQVVLAHRGSIGLGTSDSGGALFRILL"
     gene            complement(2952..3452)
                     /locus_tag="VGB57_08285"
     CDS             complement(2952..3452)
                     /locus_tag="VGB57_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0131390.1"
                     /translation="MLSQRTRYTIRALLHLADRYGEGPVQLSEIAARQNIPPKFLTVM
                     LSQMIREKLVASRRGRDGGYWLAKPPGEISYGSIVRLTRGSLGLLPCASRYRHETCKN
                     CITEDKCRLHRVMLMVRDETARILDGLSLADAIPEDAFADGSFDTGRHPGESRDPGET
                     RGGLPT"
     gene            complement(3581..5512)
                     /locus_tag="VGB57_08290"
     CDS             complement(3581..5512)
                     /locus_tag="VGB57_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY0131391.1"
                     /translation="MTAIAGFWSLDQRPDAASRCERMLKSQQIYGPQAPAMASDGPIA
                     LGQRLFGLFPEERRRQRVASGAGGATLLVADARLDNRDDLGEALGIPATEARELNDAG
                     LMLRALERWDEAAVERFQGDFAFAFWDSRRQRLLLGRDFMGQRPLHFARGNGFFAFAS
                     MPKGLHALPEIVSAPDREAVAGFLALMPEDGSETFFRGIHKVRLGEIVAVTQSGLNAT
                     RWWRPSLEPLRLKRPEDYAEALREQLDRAVAVRLRGADSAVAAHLSGGLDSSAVAATA
                     ARLMAPAGGRVTAFTGVPREDYDSSEVKNAFCDESPYAAEVAAMYPNMDHVIVRNGGS
                     PIADLDRYFFVYERPVLNLCNSVWMHRILEVARDRGLRILLSGNRGNTSFSYDGMPLL
                     TQLLSSGRFIRLARESWALVRNGTRVGSVAAQTIGPFLPIPLWHAIGRMRGKGRTLAD
                     YTSISPDAVQALRIAERAAERGLDFSYRPRRDPLETRLWSLRRVDAGNYNKGHLAGWG
                     IDIRDPTADRRLVEFCLTVPADQYLRHGVRRALARTAFADRLPARIVNETRKGYQAAD
                     WHEGLTAAREELGGEIGRIEACEEAGTTVNPAVMRELAEAMPAEGWHKPGATRKYRLG
                     LLRGVSAGHFIRKAAGSNQ"
     gene            complement(5509..5787)
                     /locus_tag="VGB57_08295"
     CDS             complement(5509..5787)
                     /locus_tag="VGB57_08295"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEY0131392.1"
                     /translation="MGEEYLVTRRGEMIETEVDGELVALHIDNGSCYGFNSTATRIWA
                     MIEQPKLISELTGELVREYDVDAATCEAEVRELLDELAADGLVEIRPA"
BASE COUNT         1007 a         2315 c         2233 g         1066 t
ORIGIN      
        1 cggacgggct tgtaggagga tatacgtggc tggggagggt aagccgttct ggcggtgcat
       61 cgtcatcgac gacgatcagg acatcctgct gtccgcacgc cttctgctcc gcgatctttt
      121 cgaagaggtc ctcaccttcc aggcgcccga ggatgcgatg gcggcggtcg agggggacgg
      181 ggccgacgtc gtcctgctcg acgcgaattt cggccggggc gcgaccaacg ccgccgaggg
      241 cttccactgg ctcggcgaga tattggcgaa ggacccgcag gccgtcgtgg tgatgatcac
      301 cgcccatggc ggggtcaaca tcgcggtcga ggcgatgaag cgcggcgcca ccgacttcgt
      361 ttccaagccc tggtcgaacg aacgcctgct cgccaccgtc cggaccgccg cttcccttcg
      421 cacctcgcgc caggcggcgg tcaccgaacg caagcgcgcc gtcgccatct ccgctccggc
      481 ggccgggggc gagacccccc tgctcggcca atccgcgccg atgcgccgca tctattcgct
      541 gatcgagcgc gccggcccga ccgaggcaaa cgtcctgatc ctcggcgaga acggcaccgg
      601 caaggagctg gtcgcgcgcg agctgcacgg caagtcgcgg cgctcgggca acgtcttcgt
      661 ctccgtggac ctgggggcgg tcgccgagaa tctgttcgaa tccgaactgt tcggccacgt
      721 caaaggcgcc ttcaccgacg cccgcggcga ccgcatcgga cggcttcagg cggcggacgg
      781 cggcaccctc ttcctcgacg aggtcggcaa cctcccgctc cacctccagc ccaagcttct
      841 gaccgcgctc gagcagcgcc aggtcactcc ggtcggggcc aacaagccgg tgtcgatcga
      901 cgtccgggtc gtcgccgcga ccaaccttcc gcccgagcgg ctggccgacg agagcctgtt
      961 ccgcccggac cttctgttcc gcctcaatac ggtcgagatc gagcttccgc cgctgcgcga
     1021 gcgccgcgag gacatccccg tcctcgtcga ccattatgtc gccctctacg ccaagaaata
     1081 tgccaggccg gcgcggcagc ttccgcccga ggtgatggcg gccctggtcg cctacgactg
     1141 gcccggcaac gtccgcgcgc tccgccacgc cgccgagcgc gccgtcatcc tcgcgggcaa
     1201 cggcgccttc accttcgagg atttctccct cacccggacg accgcgcccc gccaggcggc
     1261 gaccagcact ccgccggctc tgggcgccga cctcaacctc gagcgcgccg aaaagcagat
     1321 ggtcgagcag gccctgaagc gtaacgccta caacatctcg ctggccgcgc aggagctcgg
     1381 cctgacccga gcctcgctct accgccgaat ggacaagcat gggctttgag ggagctctaa
     1441 atcctccccg acatttgtcg gggaggggga ccgcgcgaag cgcggtggag gggttcttcg
     1501 gactgggcaa ggcccctcca ccacccttcg ggcggtcccc ctcccctgca aaggcagggg
     1561 aggattttag agggatcgat catgggcttt aggagccgtt tcgtcgccgg cctcggctgg
     1621 cgggcggcga tgctgctggg cacagtctat ctgttcgtcg ccactctggg agtggaaggg
     1681 ctcggggtcg cccgcctgct cgccgcgggc ctctgtctgt gggcgctcta cgagctgtgg
     1741 cgctacatcc agcgcaccaa catggagctc gcccgcttcc tcgaggcggt gcggctcggc
     1801 gacctgtcgc aaagcttcgc gcatcgcgcg gacggaagcg gcttcaccga tatcggacag
     1861 gcgctcgacc aggggatccg caatctgcgc gacgagcggc tgcgcctcac cgaatcgaac
     1921 cgtttctgcg aggcggtgct cgacgacgcc ccggcgccgc tcctcaccgt cgacacggaa
     1981 ggccgggtcg agctcgccaa caaggccgcg cgacggctgt tcgtccgcca ccacggagtc
     2041 cgaatcgagg attaccgtat ctatggcgag accttcgcca ccgctcttgg cgacgacgcc
     2101 atcggcaagc cgcgcctcgt ccccttgatg ctggacgagc ttccgcagac ggcgatggtc
     2161 agcgccgcgt cggtccatcg cctcggcggg atggtgaggg tcgtcgccgt ccagccgatc
     2221 cagggcgagc tgaacgcgat cgagatcgcc gcccagagcg acctcatccg ggtcctcacc
     2281 cacgagatca tgaacagcat gactccggtg acctcgctcg cccagagcgc ggccggactg
     2341 atggtggagg ccgaccgcgg cgacgatccg ctcgtttccg acgcccgggc ggcggtcgag
     2401 actctggccc ggcgcgccga cggagtcatg catttcgtcg agagctaccg ccagatcagc
     2461 cgcacccccg tcgtgcgccg ccggcatttc gaggtcctgc cctgggcgag ggagctcgaa
     2521 tccctgttcc gggccggcga aggcgcgcag ggcgtggccc tgtcgctcga cgtcgccccg
     2581 gaccggctga cgctcgacgc cgatcccgac ctcatgtgcc aggtactgat caacctgctg
     2641 cgcaatgcct ccgaggcggc ccgagaccat gcggaggcgc cggccgtctc cttatccctc
     2701 gccgcggtcg cgggcgggcg ggtccagatc gaggtggccg acaacggcgc cggagtgccg
     2761 gaggacctcc gccaggacgt cttcctcccc ttcttcacga ccaaggccaa agggacgggg
     2821 gtgggcctca gcctcgcccg ccaggtcgtc ctcgcccatc gcggctcgat cggcctcggg
     2881 acaagcgaca gcggcggggc gctgttcagg atattgctat aagccccgtc atcccggcga
     2941 aagccgggac ctcaggtcgg aaggcctccg cgggtctccc cggggtcccg gctttcgccg
     3001 ggatgacgac ccgtatcgaa agacccgtcg gcgaaggcat cctccgggat cgcatccgcc
     3061 aggctgagcc cgtcgaggat ccgcgccgtc tcgtcgcgga ccatcagcat cacccggtgg
     3121 agccggcact tgtcctcggt gatgcaattc ttgcaggttt cgtggcggta tcggctggcg
     3181 cagggaagca ggcccagcga gccccgggtg agccggacga tcgagccgta gctgatctcg
     3241 cccggcggct tggccagcca atagccgccg tcgcggccgc gccggctggc gaccagcttc
     3301 tcgcggatca tctgcgagag catcacggtc aggaatttgg gcgggatgtt ctggcgcgcg
     3361 gcgatctcgc tcagctggac cggcccttcg ccgtagcgat cggcgaggtg gaggagcgcg
     3421 cggattgtat agcgcgttcg ttgagaaagc attcccgcct catgaaccgt cccatggctc
     3481 ttggcaaccg gccccacccg tcatccccgc gaaagccggg acctgaggcc gagaggtcgc
     3541 cgcgggtctc cccgggatcc cggctttcgc cgggacgacg ttactggttc gagcctgccg
     3601 cctttcggat gaaatggccg gcggagacgc cccgcaacag gccgagccgg tatttccggg
     3661 tcgcgccggg cttgtgccag ccttccgccg gcatcgcctc ggccagctcg cgcatcacgg
     3721 cggggttgac cgtggtcccg gcctcctcgc aggcctcgat ccgcccgatc tcgccgccga
     3781 gctcctcgcg cgcggcggtg aggccttcgt gccagtccgc ggcctgatag cccttgcggg
     3841 tttcgttgac gatcctggcc ggcaggcggt cggcgaaggc ggttcgggcc agcgcccggc
     3901 ggactccatg gcgcagatac tggtccgccg gcacggtcag gcagaattcg acgaggcggc
     3961 ggtcggcggt cgggtcgcgg atgtcgatgc cccagccggc gagatgcccc ttgttgtaat
     4021 tgccggcgtc gacccgacga agcgaccaca ggcgagtctc gagcggatcg cggcggggcc
     4081 tgtagctgaa gtcgagcccc cgctcggcgg cccgctcggc gatccgcaag gcctggacgg
     4141 cgtcggggct gatcgacgta taatcggcga gcgtccggcc cttgccgcgc atccggccga
     4201 tcgcgtgcca cagcggtatc ggaaggaagg gtccgatcgt ctgcgccgcg acgcttccga
     4261 ctcgagtgcc gttgcggacc agcgcccagg attcccgggc aagcctgatg aaccgcccgc
     4321 tcgacagcaa ttgggtgagc aggggcatcc cgtcatagct gaagctcgta ttgccgcgat
     4381 tgccgctgag caggatccgg agcccgcgat cgcgcgccac ctccaggatc cggtgcatcc
     4441 acaccgagtt gcacaggttg aggacgggcc gttcgtagac gaagaagtag cggtcgaggt
     4501 cggcgatggg ggagccgccg ttgcggacga tcacatgatc catgttgggg tacatggcgg
     4561 cgacctcggc ggcatagggg ctttcgtcgc agaaggcgtt cttcacctcg ctcgaatcat
     4621 agtcttcgcg cggcaccccg gtgaaggcgg tgacccggcc cccggcgggc gccatcagcc
     4681 gggccgcggt cgcggcgacg gcgctgctgt cgaggccgcc gctcaggtgg gccgcgaccg
     4741 cggagtcggc gccgcgcagg cgaacggcga ccgcccggtc gagctgctcg cgaagcgcct
     4801 cggcataatc ttcgggccgc ttcaggcgga gcggctccag ggacggccgc caccagcgcg
     4861 tggcgttgag gccgctctgg gtgacggcga cgatctcgcc cagccggacc ttgtggattc
     4921 cccggaaaaa ggtctccgac ccgtcctcgg gcatgagggc gaggaagccg gccaccgcct
     4981 cccggtcggg cgccgacact atctcgggaa gggcgtgcag ccccttgggc atcgaggcga
     5041 aggcgaagaa gccgtttccg cgcgcgaaat ggagcggccg ctgccccatg aaatcgcggc
     5101 cgagaagcag gcgctggcgg cgcgaatccc agaaggcgaa ggcgaaatcg ccctgaaagc
     5161 gctcgaccgc cgcctcgtcc caacgctcga gcgcgcgaag catcagcccg gcgtcgttca
     5221 gctcccgggc ctccgtcgcc ggaatgccga gcgcctcgcc cagatcgtcg cgattatcga
     5281 gtcgggcgtc ggcgaccagc agggtcgcgc cgccggcccc ggaggcgacg cgttgcctgc
     5341 gccgctcctc ggggaacagg ccgaacagcc gctggccgag cgcgattggg ccgtcgctcg
     5401 ccatggcggg cgcctgcggt ccgtaaatct gctgcgactt gagcatccgc tcgcaacgcg
     5461 aggcggcgtc gggacgttga tccaggctcc aaaaacccgc gatcgcggtc atgcaggccg
     5521 gatttccacc agcccgtcgg cggcgagctc gtcgagcagc tccctgacct cggcctcgca
     5581 ggtcgctgcg tcgacgtcat attcgcgcac cagctcgccg gtcagctcgg agatcagctt
     5641 cggctgctcg atcatcgccc agatgcgggt cgcggtgctg ttgaagccgt agcagctgcc
     5701 attgtcgata tggagcgcga ccagctcgcc gtcgacctcg gtctcgatca tctctccgcg
     5761 gcgagtgacc agatattctt cgcccaccct tgcctccttg cgcgtggtgc cgcttcctgg
     5821 cgggcgacgc gggggaaccc gcgcctcgct cggcggcctg cttgtctgtg tggcgctcct
     5881 ttaccggcga agcgggcggg cgcgcaactc gcaaacccgg atttgtttgg attcccgcga
     5941 gttgcacttc gacccgccga ggactggaac cgttccatcc gcgcggagtc tgtttgacag
     6001 ctcgggcggt tctcagttac ggtccgcgct tcggataagg acagggtgac ataatgaccg
     6061 ttgtgcgtga acttcatgaa gtgaccgaag gaacccccac cctgcgcgag tggattcgcc
     6121 cgtcggttca gcagcttgcg gccgggagcg ccgaggattc ctcgggccct gccacggacg
     6181 ccatcttcaa cccctcctga atcgggtcgc cgccttgaaa tgaccgccga ctgattcgcg
     6241 gcggcatttc ttcgtccgcg gcagagccgg gcgccctcgc ggcagccgga gcatgaaaaa
     6301 ggggcgcggc caccggccgc gccccttttt tttcgcctta tcctggagcg gcgtacgcca
     6361 gatttccttc gtgccttcga aggcgaggcg gcggcggcct gataaatcct cccctgcatt
     6421 cgcaggggag ggggaccacc cgaagggtgg tggaggggcc ctttcggagc ctgaggaacc
     6481 cctccaccag gcttcgcctg gtccccctcc ccgcgaaatc gcggggagga atttagccta
     6541 aaggatgaag tcgctgctcg tgatcgggtc cggcggattg atcaccagca ccacctcgta
     6601 gtcggacact ccgtcgccgt t
//
LOCUS       DASWNU010000423         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_301413, whole genome shotgun sequence.
ACCESSION   DASWNU010000423 DASWNU010000000
VERSION     DASWNU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301413"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VGB57_08300"
     CDS             <1..1047
                     /locus_tag="VGB57_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEY0131393.1"
                     /translation="IEGIDFYRTPTPAPAPSPLREWREIKALEARLDSLAEEWKPDQL
                     HVHSPVLNALAALPVAKRRGLPLIYEIRAFWEDAAVGNGTGREGSARYRLIRMFETWA
                     ARRADAVAVICEGLRRDLVARGIPAEKIIVAPNGVDLTLFGNPLAADLKFARKLGLAG
                     ADVVGFIGSFYDYEGLDDLIAAMPLLLEKRPKAHLLLVGGGPMEEALKAQAEASPARD
                     RIHFIGRVPHHEVDRYYGLIDILAYPRKAMRLTELVTPLKPLEAMAQRKLVVASNVGG
                     HRELIEDGVTGTLFRAGDPEALTEALDTMFANRARWDERRDTAQAFVRRDRNWSSNIS
                     SYIPAYQKLTGKAV"
     gene            1047..1931
                     /locus_tag="VGB57_08305"
     CDS             1047..1931
                     /locus_tag="VGB57_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131394.1"
                     /translation="MPKLSFLSARAFDAGLAALAALSAGFVAFAMPESLFAGLVETSR
                     LPRLVAAAEPPLGETARLAAAGAAALLTFAAVWALMAALGRVPARRRPEAEPEVEAPK
                     LRRADAHPDAPARRPLLARDLGEPLDLEEFPETAIDEEGPASAEAEPRPLPAFMVPQA
                     PEPEPVAEAEPEPEYGEAEPQWAEPDPEWHSEEPEWARAEPAPEPQLEAGREFEPVPI
                     SQLAAQLPQADGEESDQSLSQLVNRIEFGLSRKRQALPAAAELPTAAEEAPVEQEKVG
                     HRLRSAINDLQKIASSGG"
     gene            complement(1937..2224)
                     /locus_tag="VGB57_08310"
     CDS             complement(1937..2224)
                     /locus_tag="VGB57_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131395.1"
                     /translation="MQAIRVQLVHFAGFALEEIRTRRWASVTFQGARHELAFRLEGEG
                     AEAAAARFLSSLDARNFALRGHLVADVSLVAQERRPGFARIRLDALTVEER"
     gene            complement(2245..2421)
                     /locus_tag="VGB57_08315"
     CDS             complement(2245..2421)
                     /locus_tag="VGB57_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131396.1"
                     /translation="MPPTRFGMEVARDPQLVFQMKQGRAVRPPMEAKILAYLDRAEQE
                     WEASPPVRRRRRRL"
     gene            2787..>4488
                     /locus_tag="VGB57_08320"
     CDS             2787..>4488
                     /locus_tag="VGB57_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0131397.1"
                     /translation="MADEERQPTRLQVATMRPEDSGRGLARLPRRLMAQLELAEGDVI
                     EITGKRATPARAVGPYTEDEGLDIIRLDGLQRANAEIGSGDFVTIRKVETKAAQRVVF
                     APAQKNLRLHGSAQALKRSFGMKPLTAGDVVATTGQQQIQRSDIPPELRQMLNAPAYA
                     LQEIRLTVVSTVPKGVVHIDANTEVELRPEYAEATETRRADVTYDDLGGIGTTIDQLR
                     EMVELPLRYPEIFERLGVDPPKGVLLYGPPGTGKTRLARAVANESDASFFHIAGPEIM
                     GSAYGESERRLREVFEEAAKASPAIIFIDEIDSIAPKRGQVQGETEKRLVAQLLTLLD
                     GLEPRQNLVVIAATNRPEALDEALRRPGRFDREIVIGVPDEGGRREILAIHTRGMPLD
                     GGVDIKELARRTFGFVGADLAALTREAAIEAVRRMMPQIKLDEGTIPQEVLDNLSVKG
                     SDFEEALKRVQPSAMREVMVQAPTIRWDDIGGVDEARERLREGVELPLKSPEAFKRMG
                     IRPAKGFLLYGPPGTGKTLLAKAAAREAEANFIATKSSDLLSKWYGESEQQIARLFAR
                     A"
BASE COUNT          755 a         1601 c         1492 g          640 t
ORIGIN      
        1 atcgagggga tcgacttcta ccggactccg acgcccgcgc cggcgccctc cccgttgcgc
       61 gaatggcggg agatcaaggc gctcgaagcg cggctcgact cgcttgcgga ggaatggaag
      121 ccggaccagc tccacgtcca ctcacccgtg ctcaacgcac tggccgccct gcccgtggcg
      181 aagcgcaggg gccttcccct catctacgag atccgggcct tctgggagga cgcggcggtc
      241 ggcaacggga cggggcgcga aggctcggcc cgctaccggc tcatccggat gttcgaaacc
      301 tgggcggcgc gccgggccga cgcggtcgcc gtgatctgcg aggggctgag gcgcgacctg
      361 gttgcccgag ggattccggc cgagaagatc atcgtcgccc ctaacggggt cgatcttacc
      421 ctgttcggca acccgctcgc cgcggatctc aaattcgccc gcaagctcgg cctcgccggc
      481 gccgacgtgg tcggcttcat cggctccttc tacgattatg agggactcga cgatctcatc
      541 gcggcgatgc cgctgcttct ggagaagcgc cccaaggcgc atctcctcct cgtcggcggc
      601 ggccccatgg aggaggcgct gaaggcgcag gccgaagcct cgcccgcgcg ggatcggatc
      661 cacttcatcg gccgggtgcc ccaccacgag gtcgaccgct attacgggct gatcgatatc
      721 ctcgcttatc cgcgcaaggc gatgcgcctc acggagctcg tgactcccct gaagccgctc
      781 gaggctatgg cgcagcgcaa gctggtcgtc gcctccaacg tcggcggcca tcgcgagctg
      841 atcgaggacg gggttaccgg aaccctgttc cgggccgggg accccgaggc gctcaccgaa
      901 gcgctcgaca cgatgttcgc caatcgcgcc cgctgggacg aaaggcgcga cacggcgcaa
      961 gccttcgtcc ggcgggatcg taactggtcg tcaaatattt ccagttacat acccgcttac
     1021 cagaagctca cgggcaaggc ggtctgatgc cgaaactctc ctttctaagc gcacgcgcat
     1081 tcgacgcggg gctcgcggcg ctggcggcgc tttccgccgg cttcgtcgct ttcgccatgc
     1141 cggaatcgct ctttgcgggc ctcgtcgaga ccagccggct tccccggctc gtcgccgcgg
     1201 ccgagccgcc gctgggcgag acggcgcggc tggccgcggc cggagcggcc gcactgctca
     1261 ccttcgccgc cgtgtgggcg ctgatggcgg cgctcggccg agtgccggcg aggcgcaggc
     1321 ccgaggccga acccgaggtc gaagccccga agctgcggcg ggccgacgcc catcccgacg
     1381 cgccggcccg gcgccctctg ctcgcccgcg atctcggcga gcccctcgat ctggaggagt
     1441 tcccggaaac cgcgatcgac gaggaggggc ccgcttccgc cgaggccgag ccgcggccgc
     1501 tgccggcctt catggtcccc caggcgccgg aacccgagcc ggtcgccgaa gcggaaccgg
     1561 agcctgagta tggcgaggcc gaaccgcaat gggccgaacc cgatcccgaa tggcactcgg
     1621 aggagccgga atgggcccgg gccgaaccgg cgccggagcc gcagctcgaa gccggcaggg
     1681 aattcgagcc ggtcccgatc tcccagctgg cggcgcaatt gccgcaagcc gatggcgagg
     1741 aatccgacca gtcgctgtcg cagctggtca accggatcga attcggactg tcgcgcaagc
     1801 gccaggccct ccctgccgcg gcggagctgc cgacggcggc ggaggaggcc ccggtcgagc
     1861 aggaaaaggt cggccaccgc ctgcgcagcg cgataaacga tcttcagaag atcgcttcca
     1921 gcggcggctg aggcgctcac ctttcctcta ccgtgagagc gtcgaggcgg atccgggcga
     1981 agcccggccg ccgctcttgc gccaccagcg acacatccgc gacaagatgg ccccgcaggg
     2041 cgaagttgcg ggcgtcgagc gagctcagga accgagcggc cgccgcctcg gcgccctcgc
     2101 cctccagccg gaaggcgagc tcgtggcggg cgccctggaa ggtgacgctg gcccaccggc
     2161 gggtccggat ctcctcgagc gcgaagccgg caaagtggac gagctggaca cggatggcct
     2221 gcaaaaacgc gctcgccgcg tcgatcagag gcggcggcgg cggcggcgga ccgggggcga
     2281 agcttcccat tcctgctcgg cccgatccag ataagcgagg atcttcgcct ccatcggcgg
     2341 gcgcacggcc cggccctgtt tcatttgaaa gacgagctga gggtcgcgcg ccacttccat
     2401 cccgaaccgg gtcgggggca ttcgggtgcg cttgagaaaa tgctctatcc ttcgtcgcaa
     2461 agaccatttc accagcactc ccctgcgaat cgctgttcgt gatatgttct attctctatt
     2521 caaatttcct atttgtctag gaactttcct accaaaatgg atgaatttcg aggcggcgga
     2581 aaatggggcg ccgacggccc cgcccgcaat gcgaacgcgt ttcagcctcc ggccacgaac
     2641 gcctcgcgcg cggaggatcg gccttcgcga agcgctccgg gtgccgcggg cggcaagggc
     2701 tttttttgcc gccgcggaac ccgccggggt ggcagagccg ttaccccaac ctatctcccc
     2761 atgacaggag cgaagggacc agcccgatgg cggacgagga aaggcagccg acgaggctgc
     2821 aggttgcgac gatgcggccg gaggacagcg gccgcggcct cgcccggctg ccgcggcggc
     2881 tcatggccca gctcgagctc gccgagggcg acgtcatcga gatcaccggc aaacgggcca
     2941 cgcccgcccg cgccgtcggc ccctatacgg aggacgaagg gctcgacatc atccgcctcg
     3001 acggcctcca gcgcgccaat gccgagatcg gctcgggcga cttcgtcacc atccggaagg
     3061 tcgagaccaa ggccgcccag cgcgtcgtct tcgccccggc gcagaagaat ctgcgtctcc
     3121 acggctccgc ccaggcgctg aagcgaagct tcgggatgaa gccgctcacc gccggcgacg
     3181 tcgtcgccac caccggccag cagcagatcc agcgaagcga catcccgccc gagctgaggc
     3241 agatgctcaa cgcccccgcc tacgcccttc aggagatccg gctgacggtg gtctccaccg
     3301 tccccaaggg cgtcgtccat atcgacgcga ataccgaggt cgagctccgc cccgaatatg
     3361 ccgaggcgac cgagacgagg cgcgccgacg tcacctatga cgatctcggc ggaattggaa
     3421 cgaccatcga ccagcttcgc gagatggtcg agctgccctt gcgctatccc gagatattcg
     3481 agcggctcgg agtcgatccg cccaagggcg tcctgctcta cggcccgccc ggcaccggca
     3541 agacccgcct cgcccgcgcc gtcgccaacg aaagcgacgc cagcttcttc cacatcgccg
     3601 ggcccgagat catgggatcg gcctatggcg agagcgagcg ccggctccgc gaggtgttcg
     3661 aggaagcggc caaggcctcg ccggcgatca tcttcatcga cgagatcgac tcgatcgcgc
     3721 caaagcgcgg ccaggtccag ggcgagaccg agaagcgcct cgtcgcccag ctactcaccc
     3781 tcctcgatgg gctcgagccg cgccagaacc tcgtcgtcat cgccgccacc aaccgccccg
     3841 aggcgctcga cgaagcgctt cgccggccgg gacgcttcga ccgcgagatc gtcatcgggg
     3901 tccccgacga aggcggtcgc cgcgagatcc tcgccatcca cacccgcggc atgccgctcg
     3961 acggcggggt cgacatcaag gagctggccc gccgcacctt cggcttcgtc ggcgccgacc
     4021 tcgccgctct gacccgcgag gcggcgatcg aggcggtccg gcggatgatg ccgcagatca
     4081 agctcgacga gggcaccatc ccccaggagg tgctcgacaa tctatcggtc aagggcagcg
     4141 acttcgagga ggccctgaag cgggtccagc cctcggcgat gcgcgaggtc atggtccagg
     4201 cgcccaccat tcgctgggac gatatcggcg gagtcgacga ggcgcgggag cggctgcgcg
     4261 agggcgtcga gcttccgctc aagagccccg aggccttcaa gcgcatgggc atacgcccgg
     4321 ccaagggctt cctcctctac gggcctccgg gaaccggcaa gactcttctg gccaaggcgg
     4381 cggcgcgcga ggccgaggcc aactttatcg ccaccaaatc ctccgacctg ctgtcgaaat
     4441 ggtatggcga gagcgagcag cagatcgctc gcctgttcgc ccgcgccc
//
LOCUS       DASWNU010000424         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_94539, whole genome shotgun sequence.
ACCESSION   DASWNU010000424 DASWNU010000000
VERSION     DASWNU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94539"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VGB57_08325"
     CDS             complement(<1..954)
                     /locus_tag="VGB57_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEY0131398.1"
                     /translation="MIDTLFSTNPAPRWPDELERIAAIARRAPFDPRAIAFVAELSGR
                     ILQSPVGREFPELAALAHWFRPASLQRMRDDQRASAGTQSLRPRGSVFIIAPANVDVL
                     FIYGWMLSLLAGNATAARVSQKPSAARDAFLGLVRELADRHRDVLEDSLLISYPHDHK
                     TTALLSGWCDLRLIWGGDNTVTAIRSIALKPTAIEGAFADRFSMAAFDAGSMAGADEG
                     ALTELARRFANDTMWFGQQACSSPRVVFWLGSDRQVAAAQDRFWPLFAQAAQRFEDEP
                     AAMMARVTDLFAMAAEGVIDHAEGDYSQLPARGRGVGPSGAV"
     gene            complement(951..2039)
                     /locus_tag="VGB57_08330"
     CDS             complement(951..2039)
                     /locus_tag="VGB57_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HEY0131399.1"
                     /translation="MTDAATALDERPFSIPSERKTAGLLEGLNALSRHHAQGSEPYRR
                     IVDAMFPASLPASALHELPWLPVQIFKRLELASVPRDRIVRTLTSSGTSGQLPSRILL
                     DRETANAQTKALVRISGDFLGKTRRPMVIIDDPSFLQDRSKFNARAAGILGFSTLGRD
                     HFYLLDSALRPDWDALDAYLDKQGDTPLLLFGFTFIVWQQFVEAAKAEGVSIRFPSGS
                     ALIHGGGWKRLEDRRVSEAAFKDALAATFGIERVHNYYGMVEQVGSIYFECENGRLHA
                     PAYSDVLIRDPKTLQPLPNGQTGLVQVLSLLPHSYPGHSLLTEDLGTVLGEDDCACGR
                     LGRYFKVHGRLKNVEMRGCSDTRSAPAS"
BASE COUNT          351 a          696 c          753 g          320 t
ORIGIN      
        1 cacggccccg ctcggcccca ccccgcggcc gcgcgccgga agctgggaat aatcgccctc
       61 ggcatggtcg atgacccctt cggcggccat ggcgaacaga tcggtgaccc gggccatcat
      121 cgccgccggc tcgtcttcga aacgctgggc ggcctgggcg aacaggggcc agaatcggtc
      181 ctgcgccgcc gcgacctgcc ggtcgctacc cagccagaac acgacccgcg gcgaggagca
      241 ggcctgctgg ccgaaccaca tcgtgtcgtt ggcgaagcgg cgggccagct cggtcagcgc
      301 cccctcgtcg gcaccggcca tggatccggc gtcgaacgcc gccatcgaaa agcggtcggc
      361 gaacgcgccc tcgatcgcgg tcggcttcag cgcgatgctc cggatcgcgg tcaccgtatt
      421 gtcgccgccc cagatgaggc gcagatcgca ccagccggac agaagcgccg tcgtcttgtg
      481 atcgtgcggg tagctgatca gcaggctgtc ctcgagaacg tcccggtgcc gatcggccag
      541 ctcccgcacc agccccagga aggcgtcgcg ggcggcgctc ggcttctgcg agacccgggc
      601 cgcggtggcg ttgccggcga gcagcgacag catccatccg tagatgaaga ggacgtcgac
      661 attggcgggc gcgatgatga acaccgaacc gcgcgggcga agcgactggg tgccggcgga
      721 ggcgcgctga tcgtcgcgca tccgctgcag gctcgccggc cggaaccagt gggccaaagc
      781 ggcaagctcc ggaaactcgc ggccgaccgg cgactgcagg atcctgcccg acaattcggc
      841 gacgaaggcg atcgcccggg gatcgaacgg cgcccgccgc gctattgcgg caatccgttc
      901 cagctcgtcc ggccagcgcg gcgccgggtt ggtggagaag agcgtgtcga tcatgaagcc
      961 ggcgcgctcc gggtgtcgct gcagcctcgc atctcgacat tcttcagccg cccgtgaacc
     1021 ttgaagtagc ggccgagccg gccgcaggcg cagtcgtctt cgccaaggac cgtccccaga
     1081 tcctccgtca gcaggctgtg gccgggatag gaatggggca gcaggctcag cacctggacg
     1141 aggccggtct gcccgttcgg gagcggctgg agcgttttgg ggtcgcggat cagcacgtcc
     1201 gaataagccg gcgcgtgcag ccggccgttc tcgcattcga aatagattga gccgacctgc
     1261 tcgaccatgc cgtaataatt gtggacccgc tcgattccga acgtcgcggc gagggcgtcc
     1321 ttgaacgcag cttccgagac ccgccggtcc tccagccttt tccacccgcc accgtggatc
     1381 agcgcgcttc ccgacgggaa gcggatcgag accccttcgg ccttcgccgc ctcgacgaac
     1441 tgctgccaga cgatgaaggt gaagccgaac agcaggagcg gggtgtctcc ctgcttgtcg
     1501 agataggcgt cgagcgcgtc ccagtcgggc cgaagcgccg agtcgagcag gtagaaatgg
     1561 tcgcggccga gggtcgagaa gccgaggatt ccggcggccc gggcgttgaa cttgctccgg
     1621 tcctgaagga aggaagggtc gtcgatgatg accatcgggc ggcgggtttt gccgaggaaa
     1681 tcgccggaga tgcgcaccag cgccttggtc tgggcattgg cggtctcccg gtcgaggagg
     1741 atccgcgacg gcaactggcc gctggttccg ctcgaggtca gggtgcggac gatccggtcg
     1801 cgcgggacgc tggccaattc cagccgcttg aagatctgca ccggcagcca cggcagctcg
     1861 tgcagcgccg aagctgggag gctggccgga aacatcgcgt cgacgatccg acgatagggc
     1921 tcggatccct gcgcgtggtg cctcgacaga gcgttgaggc cttcgagcag cccggccgtc
     1981 ttgcgctcgc tcgggatgga gaaagggcgc tcgtcgagcg cggtggcggc gtcggtcacg
     2041 gggactctcg gtcgttcgag cggagggcga gcgagcgata gtcgattttc ccgttcgccg
     2101 tcagcgggaa agtggcgatc
//
LOCUS       DASWNU010000425         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_405454, whole genome shotgun sequence.
ACCESSION   DASWNU010000425 DASWNU010000000
VERSION     DASWNU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405454"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VGB57_08335"
     CDS             complement(<1..873)
                     /locus_tag="VGB57_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0131400.1"
                     /translation="MKKLSLLWATTALVMPVTAFAQSTGSQEVEEEIVITGSRNNDGV
                     DGIVVPDSTKAKGVITQELISRQGAGQTILNTINLLPSVNFTQSDPYGSAGGNIRIRG
                     FDGNRISLTFDGLPLNDTGNYAIFSNQQLDPELIEQVNVNFGATDVDSPTASAAGGTI
                     NYRTIIPTRDFGARVAASWGEDEYHRLFGIVQTGELTSFGTRAFLSASFAKNDKFKGP
                     GQIYKQQYNARIYQPLGGDDFISIAGHWNQNRNNFYRNPSINDLRTLFGAARFPASAS
                     ATPENPLVVDGFSGV"
     gene            complement(1040..1675)
                     /locus_tag="VGB57_08340"
     CDS             complement(1040..1675)
                     /locus_tag="VGB57_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY0131401.1"
                     /translation="MPRSLFAYSIFYGGMVCIAGVLANKQVALGPLGVEAGIFAFLLL
                     VVASSAVAELFGRETANRLVLIGFAPLIFSILLAIFVLKLPAAHDMPADRLAAFTTIM
                     GGTPRIWIAGIIAYGVSQFLNVTIFSALRGKGEGRMVWLRAGIAAVLSQIVDTLLFIT
                     ISFYGLFPIADLLLGQMLAKVVLSIVLVPPLVSLAVAFGRRLDGPLSAGRT"
     gene            1856..>2659
                     /locus_tag="VGB57_08345"
     CDS             1856..>2659
                     /locus_tag="VGB57_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY0131402.1"
                     /translation="MTDTRLDVLAIGDAVVDVIARAEDSFLAEERLVKGSMQVLDADG
                     ATRLYSKMSQARETSGGSAANTVAGVAALGGRAGFVGQVAADQLGEIFIHDIRALGVE
                     FVTPPKKDGLPTGRCLILVTPDAQRTMNTFRGAAHQLTVDALDAEQIRGSAILYLEAY
                     LWRSEGPRAAMDEAMRIAREAGRKVAFTLSDIACIGPHRREIVAMLDSGAIDLLFANE
                     MEIKVLADLDDMASAIAAIQDKVRVLVVTCGAEGALAVEGGRRVSVPVAR"
BASE COUNT          479 a          825 c          932 g          423 t
ORIGIN      
        1 gacgccgctg aagccgtcga cgacgagcgg attttcggga gtcgcggaag cgctggccgg
       61 gaacctggcg gcgccgaaca acgtccggag atcattgatc gacgggttgc gatagaaatt
      121 gttgcggttc tggttccagt ggcccgcgat cgagatgaag tcgtcgccgc cgagcggctg
      181 gtagatgcgg gcgttatact gctgcttgta gatttggccc ggacccttga acttgtcgtt
      241 cttggcaaag ctggcggaca ggaaggcgcg ggtgccgaag ctggtcagct ccccggtctg
      301 aacgattccg aacaggcggt gatattcgtc ctcgccccag gaagcggcaa cgcgcgctcc
      361 gaagtcgcgg gtcgggatga tcgtgcgata gttgatggtg ccgccggccg ccgaggcggt
      421 cgggctgtcg acgtcggtgg cgccgaagtt gacgttcacc tgctcgatca gctccggatc
      481 cagctgctgg ttcgagaaga tcgcgtaatt gccggtgtcg ttcaggggca ggccgtcgaa
      541 ggtcagcgag atccggttgc cgtcgaagcc gcggatgcgg atattgccgc cggccgagcc
      601 gtagggatcc gattgggtga agttgacgct gggcagcaga ttgatcgtgt tgaggatggt
      661 ctggccggcg ccctgccgcg agatcagctc ctgggtgatg acgcccttcg ccttggtcga
      721 atcgggaacg acgatgccgt cgacaccgtc gttgttgcgg gagccggtga tgacgatctc
      781 ttcctcgacc tcctgcgaac cggtggactg agcgaaggcc gttaccggca tgacgagcgc
      841 cgtggtcgcc caaagcagcg agagcttctt catggtagct aattcccctc ggaggccgaa
      901 tcgcctcgtt cccggcgcct gctatagccg caatggggca aatcgaagac tcaaactgca
      961 cccgccggga aaaaagttat catgggttgc agcgctgccg caccgctacc taagtatctg
     1021 attacattag tggaacaaat caggttcgac cggcgctgag aggcccgtcg aggcgccgcc
     1081 cgaaggccac ggcaagcgag acgagcggcg gcaccaggac gatggagagc accaccttcg
     1141 ccagcatctg gccgagcagc agatcggcga tcgggaacag tccgtagaag gatatggtga
     1201 tgaagagcag ggtgtcgacg atctgcgaaa gcaccgcggc gatgcccgcg cggagccaga
     1261 ccattcgccc ctcccccttg ccgcgaaggg cgctgaagat ggtgacgttg aggaattggg
     1321 agaccccgta ggcgatgatc cccgcgatcc agatgcgggg agtgccgccc atgatcgtgg
     1381 tgaaggcggc gagccggtcc gccggcatgt cgtgcgcggc cggcagcttg agcacgaaga
     1441 tcgcgagcag gatcgagaag atgagcggcg cgaagccgat caggacgagc cggttcgccg
     1501 tctcccgccc gaacagctcg gcgaccgcgc tcgaggcgac gacgaggagc aggaaggcga
     1561 agattccggc ctccacgccc agcgggccga gcgcgacctg cttgttggcg aggacgcccg
     1621 cgatgcacac catgccgccg tagaagatcg aataagcgaa cagggagcgg ggcatgcctc
     1681 ctcccggcgc tccagaggag gcggcggagg cggaggcggc cgcggcggct gggacttctg
     1741 tggtcatggg ccgtgcctaa cggctttcgc ggccagcaaa aagggctttc cgtcgagcca
     1801 ttctccggcc atctaggcgg cccttcctga tccagatcac gaaagtccga gctccttgac
     1861 cgatacccgc ctcgacgtgc ttgcgatcgg cgacgccgtg gtcgacgtca tcgcccgggc
     1921 ggaggacagc ttcctcgccg aggaaagact ggtcaaaggc tcgatgcagg tgctcgacgc
     1981 cgacggagcg acgaggctct actcgaagat gagccaggcg cgcgagacga gcggaggctc
     2041 ggccgcgaac acggtggcgg gcgtggcggc gctcggcggc cgggccgggt tcgtcggcca
     2101 ggtcgccgcc gaccagctgg gcgagatctt catccacgac atccgcgctc tgggagtcga
     2161 gttcgtcact ccgccgaaga aggacggact ccccaccggc cgctgcctca tcctcgtcac
     2221 gcccgacgcc cagcggacga tgaacacctt ccggggcgcc gcccaccagc tcaccgtcga
     2281 cgcgctcgac gccgagcaga tcaggggctc ggcgatcctc tacctcgaag cctatctgtg
     2341 gcgctcggaa gggccgcggg cggcgatgga cgaggcgatg cggatcgccc gcgaggccgg
     2401 ccgcaaggtc gccttcaccc tctccgacat cgcctgcatc ggcccgcacc gacgggagat
     2461 cgtggcgatg ctggacagcg gcgccatcga cctcctcttc gccaacgaga tggagatcaa
     2521 ggttctcgcc gacctggacg acatggcgtc ggccatcgcc gccatccagg acaaggtgcg
     2581 ggtgctggtc gtgacctgcg gcgccgaagg cgcgcttgcg gtcgagggcg gccgccgggt
     2641 cagcgtgccg gtcgcccgg
//
LOCUS       DASWNU010000426         8335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_94584, whole genome shotgun sequence.
ACCESSION   DASWNU010000426 DASWNU010000000
VERSION     DASWNU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94584"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VGB57_08350"
     CDS             complement(<1..400)
                     /locus_tag="VGB57_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131403.1"
                     /translation="MTTNKKCPVDIEYGDLQGNVLTAYGRLGFPYGRFGVLTVHDAAA
                     GRAFVERLRPRVTTALRWPSRAKAITEGRKTVERPPVTINLAFTFRGLLALGVPTRTL
                     RGMPDEYIDGMAARCAILGDDTPENPKSGWD"
     gene            complement(397..1341)
                     /locus_tag="VGB57_08355"
     CDS             complement(397..1341)
                     /locus_tag="VGB57_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131404.1"
                     /translation="MPNFDGGHYFYTGLAPINRTAVPGPDGYMTAPSHIVRQALQTLP
                     NFSQVPGSSRTSPFARSESTHFARFAVIDDPAFNGRDPGNAIRQGLTGVDLLAHQPVD
                     YLSRPWLLFGADFDASDGKDSTRDEWAEDLWRHMSSELRAVFGHCTDFDAVTDGKGFA
                     RYLARAQVPTTMSFNDYYIDKLNLPTLGITRLLVQIAVPVVLFLVAAWLIDREAEVGW
                     WLWALAALLGLGVGVWAAYRLIMSRGAAPFPPAPGGEGRLETVLKGLYLQQRFTQFAT
                     DHQLDTPEQLHAAFGTFLADHDPADSSKPTQAPGVLKS"
     gene            complement(1341..2030)
                     /locus_tag="VGB57_08360"
     CDS             complement(1341..2030)
                     /locus_tag="VGB57_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131405.1"
                     /translation="MSNVAGKAYAMNVVTPMRPARTWINTLIFMFARAQPEKLGGLLG
                     LSIIHFARWVMIRRDNWPDFGQPGEKLENDYMLFLSNFNGTWDQYIDAFSDGLPNGLD
                     MFWYSSTKYPHSIPISPFKNYIRANQIDTDYYYNATPGSAQRDVKAGLRVRRMLLALA
                     GEHERLDPRAFAEAYRKALVQVQNDLGAQGEAPVASNDTRDADINRQPYVDKAWPAAI
                     LETEAMTGRAV"
     gene            complement(2120..3340)
                     /locus_tag="VGB57_08365"
     CDS             complement(2120..3340)
                     /locus_tag="VGB57_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY0131406.1"
                     /translation="MSSNELVLRRPIMARVPELRHWLASAVLATMAGLYLVAGLSLAW
                     LDPASATPNARLAGTERGIPGLGIPPQPEPLQFRDVAPQDAVAINAAVPVSTLPNPAA
                     RPFGAAFATPADRLRAVECLTAAVYYEAAIESTDGQRAVAQVVLNRARHPAYPRTVCG
                     VVFQGQERATGCQFTFTCDGALRRTPSAAGWARARRVAEEALAGKVYAPVGWATHYHT
                     NWVVPYWSSSLTKLGNVGTHIFYRWEGGWGKPAAFRFSPGRVEPQIAKMRSLTSDPTS
                     LADAPTIDDAAALAAAEAAAAAAAATAAAEVGTKPPGSIDSFTRAVLRRYEPPNAEAS
                     TAAVQVRSGKPLSASTHWALSGVPAKGETGAAAKPPAPPAKPAAAAEAAPKCLDGVRR
                     VLSGKAVSEKTPGC"
     gene            complement(3486..4634)
                     /gene="tgt"
                     /locus_tag="VGB57_08370"
     CDS             complement(3486..4634)
                     /gene="tgt"
                     /locus_tag="VGB57_08370"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0131407.1"
                     /translation="MAARPRFAFTISAIDGAARTGTISMRRGEIRTPAFMPVGTAATV
                     KAMKPADVRAAGADIILGNTYHLMLRPGAERVARLGGLHDFMGWERPILTDSGGYQVM
                     SLSDLAKVTEEGVAFKSHLDGSRHMISPERSMEIQRLLGSDIVMAFDQLVPTTSTRDE
                     QAAAMDRSMRWARRSREAFDSGEEHASWAALFGIQQGALDEGLRRASAEALTDIGFDG
                     YAVGGLAVGEGQEAMFGCLDFAPGQLPADRPRYLMGVGKPDDIVGAVERGIDMFDCVL
                     PTRSGRTGQAFTRGGPVNIRNARFAEDSGPLDSACPCPVCATWSRAYLHHLVRSGEIL
                     GAMLMTEHNIWFYQGLMSDLRAAIQERRLAAFAAGFRALYRSSRAPEG"
     gene            complement(4755..5159)
                     /locus_tag="VGB57_08375"
     CDS             complement(4755..5159)
                     /locus_tag="VGB57_08375"
                     /inference="COORDINATES: protein motif:HMM:NF039318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6006 family protein"
                     /protein_id="HEY0131408.1"
                     /translation="MSIKLLATAALFAAALAASAPAAASQVAGAWYMGDWRCTLDGRP
                     TRIVWDLVNVDHGSHHGDVGTSVAGVERRGRFWDRGSWANLSLGRATATTVHFKHADG
                     NNWFLRRISATQARGNSTWNGRPYPLSCTKAR"
     gene            complement(5256..5591)
                     /locus_tag="VGB57_08380"
     CDS             complement(5256..5591)
                     /locus_tag="VGB57_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0131409.1"
                     /translation="MQRVFEALSSTVRRKILAYLAHADLTAGEIAARFEMSKSAVSQH
                     LGILENAGLVASEKKGQFVHYRLVPDSLANTLNTYLQQVCPVSGPLKKESAGLATKKV
                     AGADGVEEA"
     gene            5679..6620
                     /locus_tag="VGB57_08385"
     CDS             5679..6620
                     /locus_tag="VGB57_08385"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY0131410.1"
                     /translation="MSESDPHAVNSLRAGAADPSSGRVRWDPVHSLWNGVMLAAALTL
                     GPLTFSPSAFLVFALSTGAGLLLGHSVGFHRRLIHGSFDCPPWLERVLVWFGTSVGMS
                     GPFWMIRTHDLRDWAQRQPHCHDYLAHRRPMLVDAWWQIHCRLELDRPPSFDLGRIGR
                     DPFYRFLERTWMLQQAPIALALFWLGGWSWVVWGVCARVAASVTGHWFVGHLAHRRGP
                     QSWLVDGAGVQAHDVPWAAIPTMGEAWHNNHHAFPGSARIGLYPGQADWGWLFIRLLE
                     RLGLAWNIRTPETLAARAALTAAPPRYRPGSKRLASR"
     gene            complement(6697..7620)
                     /locus_tag="VGB57_08390"
     CDS             complement(6697..7620)
                     /locus_tag="VGB57_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131411.1"
                     /translation="MNDRRDNGTPVDGRPRKPRIEVLQRGPAAAPGPRSTQDPNSNDI
                     DGASTAAPGAPAARSSWTAHQPPQAETASAGRRGGLSRSPTTLLILLACIVIAGFLLY
                     TYLSSERGAEYSDKGTEMSSADADLVGEPRSDSDAATAQQPAEPPAVASVDDGAAPEP
                     EPIASSAPQPAPASASAPEDQPAAADRPESPPAARTVVEDGNSGASDTVRAFYSALSA
                     GDGRSAAQLVVPAKRRSGPLSAGALTRYYSSFRRPLRVRRVDPLDANTVRVAYDYVLA
                     DGRVCRGQAAVDVVESEGRSLVSGIRTRGPC"
     gene            complement(7617..8297)
                     /locus_tag="VGB57_08395"
     CDS             complement(7617..8297)
                     /locus_tag="VGB57_08395"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEY0131412.1"
                     /translation="MRAQVSLVHADVYDRSVASLQALRTVDLTPEERSACEGKLATGE
                     YLLLAQPRSGEGPDNIISVLERVAAEPLRETHSRAPSQGTGTAGSGPSVIAEERIPLV
                     EEELQVGTREVVRGGARVRTRVEEVPVAQEIELMSEFVRVASRPASRPVSEQELEHGG
                     LLRDRVIEIAQVREEAVVSKEVVVREEVVVTKTTERRVEHIHESVRRTVVETEELGNE
                     AAGGRGQP"
BASE COUNT         1311 a         2819 c         2814 g         1391 t
ORIGIN      
        1 gatcccaacc cgacttcgga ttttcgggcg tgtcgtcgcc gaggatcgcg catcgcgccg
       61 ccattccgtc gatatactcg tcgggcatgc cgcgcaaagt ccgggtcgga acgcccagcg
      121 cgagaaggcc gcggaaggtg aaggcgaggt tgatcgtcac cggcggccgc tcgaccgtct
      181 tgcgtccctc cgtgatcgcc ttggcgcgcg agggccagcg cagcgccgtc gtgacccggg
      241 gcctgagccg ctcgacgaac gcccgccccg cggcggcgtc gtgcaccgtc aggactccga
      301 aacggccata ggggaagccg agcctgccgt aggcggtgag gacattgccc tggagatcgc
      361 catattcgat gtcgacgggg catttcttgt tcgtggtcat gatttgagca cccccggcgc
      421 ctgggtcggc ttgctgctgt cggccgggtc gtgatcggcg aggaaggtgc cgaacgcagc
      481 gtgcagctgc tccggcgtgt cgagttgatg atcggtcgcg aactgggtga accgttgctg
      541 caggtagagg cccttgagca ccgtctcgag cctgccttcg ccgccgggcg ccggcgggaa
      601 gggagcggcc ccgcggctca tgatcaggcg gtaggctgcc cagacgccga cgcccaggcc
      661 caacagggcc gcaagggccc aaagccacca gccaacctcg gcctcgcggt cgatcagcca
      721 ggcagcgacc aggaacagca ccaccggcac cgcgatctgg acgaggaggc gagtgatgcc
      781 aagcgtcggc aggttgagct tgtcgatata ataatcgttg aacgacatgg tggtcgggac
      841 ctgggcccga gcaagatagc gggcgaagcc cttcccgtcc gtgactgcgt cgaagtccgt
      901 gcaatggccg aacaccgctc gaagttcgga gctcatgtgc cgccagagat cctcggccca
      961 ttcgtcgcgc gtggagtcct tgccgtcgga agcgtcgaag tcggctccga acagaagcca
     1021 cggccgggac agataatcga ccggctggtg ggcgagcagg tcgactccgg tcagcccctg
     1081 cctgatcgcg ttcccggggt cgcggccgtt gaacgcggga tcgtcgatga cggcgaagcg
     1141 ggcgaaatgg gtgctctcgg agcgggcgaa cgggctggtt cgggacgatc ccggcacctg
     1201 gctgaaattg ggaagcgtct ggagcgcctg gcggacgata tggctgggcg ccgtcatgta
     1261 gccgtccggc ccgggcaccg ccgtccggtt gatcggggcg agaccggtat agaaataatg
     1321 gccaccgtcg aaattgggca tcagaccgcc ctccccgtca tcgcttcggt ctcgaggatc
     1381 gcggccggcc aggctttgtc aacataaggc tggcggttga tgtcggcgtc cctggtgtcg
     1441 ttggaggcga cgggcgcttc gccctgggca ccgaggtcgt tctgcacctg gacgagcgcc
     1501 ttgcgatagg cctcggcgaa ggcccgggga tcgagcctct catgctcccc ggccaaagcg
     1561 aggagcatcc gcctgaccct cagccccgcc ttgacgtccc gctgagcgga gccgggcgtc
     1621 gcgttgtaat aataatcggt gtcgatctgg ttggcgcgga tgtagttctt gaacgggctg
     1681 atcggaatgg aatgcggata tttggtgctc gaataccaga acatgtcgag cccgttcggc
     1741 agtccgtccg agaaggcgtc gatatattga tcccaggtgc cgttgaagtt cgacaggaag
     1801 agcatgtaat cgttttcgag cttctccccg ggctggccga aatccggcca gttgtcgcgc
     1861 ctgatcatca cccagcgggc gaagtggatg atcgagagtc cgagcagccc gcccagcttc
     1921 tcgggctggg cgcgggcgaa catgaagatc agagtgttga tccaggtgcg cgcgggacgc
     1981 atcggcgtca ccacgttcat cgcatatgct ttgccggcta cgttcgacat ggcgccccct
     2041 ccacgatgtg cgaagggtga gccgaaaagg gcttcaggtc aaagccaatt gcatccgaag
     2101 cagcaggttg ccgacttgat cagcagcccg gggtcttctc cgagaccgcc ttgccgctca
     2161 gaacccggcg aaccccgtcg aggcacttcg gagccgcttc ggccgccgcc gccggcttgg
     2221 cgggcggtgc cggcggcttg gcggcggcgc ccgtctcgcc cttggccgga accccggaga
     2281 gcgcccagtg cgtgctcgcc gagagcggct tgccggagcg gacctgaacc gcggcggtcg
     2341 aagcctcggc gttcggcggc tcgtagcggc gaagcacggc gcgggtgaag ctgtcgatgc
     2401 tgcccggggg cttggtcccg acctccgcgg cggcggtggc tgcggcggcg gcggcggcag
     2461 cctccgccgc ggccaaagcg gcggcgtcgt cgatcgtcgg agcgtcggcg aggctggtcg
     2521 ggtcggaagt caggctgcgc atcttggcga tctgaggctc gacccggccg gggctgaagc
     2581 ggaatgcggc cggtttgccc cagccgcctt cccagcggta gaagatgtgg gtgccgacat
     2641 tgccgagctt ggtgaggctc gagctccaat agggcacgac ccagttggtg tgataatgag
     2701 tcgcccagcc caccggcgca tagaccttgc cggcgagcgc ttcctcggcg acccggcgcg
     2761 cgcgcgccca gcccgccgcc gacggcgttc gacgaagcgc gccgtcgcag gtgaaggtga
     2821 actggcaccc ggtcgcccgc tcctggccct ggaagaccac gccgcagacg gtgcgcggat
     2881 aggccggatg gcgggcccgg ttcaggacca cctgggcgac cgcgcgctga ccgtcggtcg
     2941 attcgatggc ggcttcgtaa tagacggcgg cggtcaggca ttcgaccgcg cgcaggcgat
     3001 cggcgggcgt cgcgaaggcg gcgccgaacg gccgggcggc gggattgggg agggtcgaga
     3061 ccggtaccgc ggcgttgatc gccaccgcgt cctgaggcgc gacgtcgcgg aactggagcg
     3121 gctcaggctg gggcggaatg cccaggcccg gaatccctcg ctcggtcccc gcgagacgcg
     3181 cgttcggggt cgcgctcgcc gggtcgagcc aggcgaggct gagcccggcc accagataca
     3241 ggccggccat ggtcgccagg acggcggacg cgagccagtg cctcagctcc gggacccggg
     3301 ccatgatcgg acggcgcagg acgagttcgt tcgacgacat caactactcc ggaaaggacg
     3361 ggcgcactat tgcgaccgcc gatccatggc aagcgcgagg ccgctcaacc ggtccctcgt
     3421 cgcgccgaag atcctggaat tccaccggcc gctctctacc ccagatgggg ataaatctca
     3481 aattttcaac cttcgggggc tcggctggac cggtagagag cgcggaaacc ggcggcgaaa
     3541 gcggcgagtc ggcgctcctg tatggccgct cgcaaatcgc tcataagtcc ttggtaaaac
     3601 cagatattgt gctccgtcat cagcatcgcc cccaatatct ctccggaacg gacgaggtgg
     3661 tggagatagg ctcgcgacca tgtcgcgcag accgggcagg ggcaggccga atcgagcggg
     3721 ccggaatcct cggcgaagcg ggcgtttcgg atgttgacgg ggccgccccg ggtgaaggcc
     3781 tggccggtcc ggcccgagcg ggttggaaga acgcagtcga acatgtcgat cccccgctcc
     3841 accgctccga cgatgtcgtc gggcttgccc actcccatca aatagcgcgg ccgatccgcc
     3901 gggagctgcc ccggcgcgaa atcgaggcag ccgaacatcg cctcctgccc ctcccccacc
     3961 gccaggccgc cgaccgcata gccgtcaaag ccgatatcgg tgagggcctc ggccgaagcc
     4021 cggcgcagcc cttcgtcgag cgcgccctgc tggatcccga acaaggcggc ccacgaggca
     4081 tgctcttcgc cggagtcgaa cgcctcccgc gatcgccgag cccagcgcat cgaacgatcc
     4141 atcgccgccg cctgctcgtc ccgcgtcgat gtggtcggca cgagctggtc gaaagccatg
     4201 acgatgtcgg agccgagaag cctctggatc tccatcgacc gctccgggct gatcatgtgc
     4261 cggctgccgt ccaggtggct tttgaacgcc acgccctctt ccgtgacctt ggccaggtcc
     4321 gacaggctca tcacctgata gccgccgctg tcggtcagga tcggccgctc ccagcccatg
     4381 aaatcgtgca gcccgcccag cctcgctacc cgctccgccc cgggccggag catgaggtgg
     4441 taggtattgc ccaggatgat gtcggcgccc gcggcgcgga cgtccgccgg cttcatcgcc
     4501 ttgacggtcg cggcggtccc caccggcatg aaggccgggg tccggatctc gccccggcgc
     4561 atcgagatcg tgccggtgcg tgcggcgccg tctatggcgg agatcgtgaa ggcgaagcgg
     4621 ggacgtgcgg ccatggccgc gcgctcctac accatcgccc tcctcccttc acccctctcc
     4681 ctccagggga gagggacagg cgcgaagcgc cagggtgagg gcaccccgat ccagcagccg
     4741 gactcaaccc gggctcagcg cgccttcgtg caggaaagcg ggtatggccg cccgttccag
     4801 gtcgaattgc ccctggcctg ggtcgccgag atccgccgca gaaaccagtt gttgccgtcg
     4861 gcatgcttga agtggacggt ggtcgcggtg gcacggccga ggctcagatt ggcccacgat
     4921 ccccgatccc agaaccgccc ccgccgctcc actccggcga cgctggtgcc gacatcgccg
     4981 tggtggctgc catggtcgac gttcaccagg tcccagacga tccgcgtcgg ccgtccgtcg
     5041 agcgtgcacc gccagtcgcc catgtaccag gcgccagcca cctggctcgc ggccgctgga
     5101 gcgcttgccg cgagggccgc ggcgaacaag gctgccgtcg ccaaaagctt gatgctcatg
     5161 atcatcctcc ctcggccctc gggcccaagc cctgtcctta cccgaaacga cccgaggaga
     5221 gtaggcggct ttgtcgtgga aacaggaggc ccggctcagg cttcttccac gccgtcggct
     5281 ccggcaacct tcttcgtagc gaggccggcg ctctccttct tgagcgggcc tgaaacgggg
     5341 cagacctgct ggagataggt gttgagggtg ttggcgaggc tgtccggcac cagccggtaa
     5401 tggacgaact gccccttctt ctcgctcgcc accagtccgg cattttcaag aattccgaga
     5461 tgctgggaca ccgcggattt cgacatttca aagcgcgccg cgatctcgcc cgcggtgagg
     5521 tcggcatggg ccagataggc gaggatcttg cgcctgacgg tggatgagag ggcttcgaag
     5581 acgcgttgca tggaggagga gttaagcagt tgcttaagca cttgcaaggc tgacgagata
     5641 ggagttaagt gatgacttaa ttgcttagga gcttcagcat gtccgagtcg gacccccatg
     5701 ccgtgaattc cctccgcgcc ggcgccgccg atccctcgtc tggacgggtt cgctgggacc
     5761 ctgtccactc gctgtggaac ggcgtcatgc tcgcggcggc gctgacgctc ggcccgctca
     5821 ccttctcccc gtccgccttc ctggtcttcg ccctgtccac gggggccggg ctgctcctcg
     5881 gccattcggt gggcttccac cggcggctga tccacggcag cttcgattgc ccgccgtggc
     5941 tggagcgcgt gctcgtctgg ttcggaacga gcgtcgggat gagcgggcct ttctggatga
     6001 tccgcaccca cgacctgcgc gactgggcgc agcgccagcc gcattgccac gattacctcg
     6061 cccaccggag accgatgctc gtcgacgcct ggtggcagat ccactgccgc ctggagctcg
     6121 atcgtccgcc ttcattcgat ctgggccgga tcggccgcga ccccttctac cgcttcctcg
     6181 agcggacctg gatgctccag caggcgccga tcgccctcgc cttgttctgg ctcggaggct
     6241 ggtcctgggt ggtctggggc gtctgcgcgc gggtcgccgc ctccgtcacc gggcattggt
     6301 tcgtcggcca cctcgcccat cgccgcggcc cgcaaagctg gctggtggac ggcgcgggag
     6361 tccaggccca cgacgtcccc tgggccgcga tcccgacgat gggggaggcg tggcacaaca
     6421 accaccacgc cttccccggc tccgccagga tcggccttta ccccggccag gccgactggg
     6481 gctggctgtt catccgcctg ctggagcgcc tcggcctcgc ctggaacatc cgcaccccgg
     6541 agaccctggc ggctcgagcg gcgctcactg cggcgccgcc gcgctaccgg ccgggcagca
     6601 aaaggctggc atcgcgatga tcgcatggcc gtgcccggtt cgggcaggct tggtgccagg
     6661 caggtagctg gcacagtggc ggaatacgat ttgattttag cagggcccgc gcgttctgat
     6721 accgcttacc aggctcctgc cctcactttc gaccacgtcg actgccgcct gtccgcgaca
     6781 aacccgcccg tcggccagca cataatcata cgccactcga accgtgttgg cgtccagagg
     6841 gtccacgcgc cgcacccgca gcgggcgacg gaacgaggag taatagcggg tcagcgcccc
     6901 cgcggagagc ggcccgctcc gccgcttcgc aggtacgacc aactgcgcgg ctgacctgcc
     6961 gtctccggcg ctcagagccg agtaaaaggc gcgaacggtg tccgaggctc cggaattgcc
     7021 gtcttcgacc acggttctgg cggccggagg agattccggc ctgtcggcgg cggccggctg
     7081 atcttccggc gcggaggcac tcgccggtgc cggctggggt gctgacgaag caataggttc
     7141 gggttcgggc gccgctccat cgtcgacgct tgcgacggct ggcggttcgg ccggctgttg
     7201 ggccgttgcc gcgtcggaat cgctccgggg ctctcccaca agatccgcat ctgccgagct
     7261 catttccgtc cccttgtcgg aatactccgc accacgttcg ctcgaaagat aagtgtacag
     7321 caggaagccg gcgatcacga tgcaggccag gagaatcagc agcgttgtcg gcgaacgcga
     7381 cagccctccg cgcctccccg ccgaggccgt ctcggcctgc ggaggctggt gggcagtcca
     7441 ggaggaccga gccgccggtg ctcccggcgc ggcggtgctc gctccgtcga tgtcgttgct
     7501 gttgggatct tgtgtggacc ggggccccgg agccgctgcc gggccccgct gcagcacctc
     7561 gatgcggggc ttgcggggcc gcccatcgac gggcgtgcca ttgtcgcgac ggtcgttcat
     7621 ggctgccctc gtccgcccgc ggcctcgttg ccaagttcct ccgtttcaac cacggtgcgg
     7681 cgaacgcttt cgtggatatg ctcgacacgg cgctccgtag tcttggtcac cacgacttcc
     7741 tcgcgaacga cgacctcctt gctcacaacg gcctcttccc ggacctgtgc aatctcgatc
     7801 acccggtcgc gaagcaggcc gccatgttcc aattcctgct cgctgactgg ccgcgatgcg
     7861 gggcggctcg cgacgcgcac gaactcgctc atcaactcga tttcctgcgc aaccggaacc
     7921 tcttccaccc gggtccggac tctcgccccg ccacgaacga cctcgcgggt tcctacctgc
     7981 agctcctcct cgaccagcgg aatccgctcc tcggcaatga ccgaagggcc gcttcccgca
     8041 gttccggtcc cctgtgaagg tgcgcgggag tgggtctctc gaagcggctc ggcggctacg
     8101 cgctcaagga ccgaaattat attatccggg ccctccccgc ttcggggctg ggcaaggagc
     8161 agatattctc cggtggccag cttgccctcg caagccgatc gctcctccgg tgtaagatcg
     8221 acagttcgca gggcctggag gctcgcaacc gagcgatcgt aaacgtcggc atggacaagc
     8281 gaaacctgag ccctcaaagc ctgcagggcc cgggcggcct cctcgcgcga atcgt
//
LOCUS       DASWNU010000427         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_301557, whole genome shotgun sequence.
ACCESSION   DASWNU010000427 DASWNU010000000
VERSION     DASWNU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301557"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VGB57_08400"
     CDS             complement(<1..360)
                     /locus_tag="VGB57_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0131413.1"
                     /translation="MRAFQHEEHNFSLSGLMARALLVVAAALTVAGCASRGGSVPYEP
                     TGFQAPDTQGQVVANASHPIGALDTISVSVFQVESLSGDYKVDAAGKIDYPLLGSVQA
                     QGRTTQELRQQMAAALSQ"
     gene            627..1406
                     /locus_tag="VGB57_08405"
     CDS             627..1406
                     /locus_tag="VGB57_08405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0131414.1"
                     /translation="MLSRLFSKSRTSRGRSGPPGSRAYAIGDVHGRLDLLVDLLARIE
                     EDNARRGPAKTWLVFLGDLVDRGPDSRGVVDLLVSRPPGFARNVFLKGNHEEFFLGVL
                     GGDDSSVQHWLAYGGTECAQSYGLTTGWMLNATPAAIMERLIRQVPESHVRFLDEMHD
                     SFRFGDYLFVHAGIRPGVALDKQVSRDLRWIREGFLDDRSDHGVVVVHGHTIVERAEE
                     HPNRIAVDTGAYRSGTLTAIGLEGEERWFIEARETGAAAAA"
     gene            1448..2443
                     /locus_tag="VGB57_08410"
     CDS             1448..2443
                     /locus_tag="VGB57_08410"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0131415.1"
                     /translation="MKILVTGAAGFIGHHLGARLLARGDEVVGIDNFNDYYSVALKRD
                     REAKLRRDFGGFDLLEVDFADEAALDRALDGIEIDRIVHLGAQAGVRYSIENPRAYVQ
                     SNLVGHVNMLEIARHRGLSHMVYASSSSVYGGNDTLPFRVEDRVDHPLSLYAATKKAD
                     ELMSETYAHLYRLPQTGLRFFTVYGPWGRPDMAMWLFTKAIFAGEPIQVFGEGNMRRD
                     FTYVDDIVTGVVAALDNPPPDDGEIKAGGSRGPHRLYNIGNHRSEELTRMIGLIEQAC
                     GREAEKILMPMQPGDVRDTYADISAIQNDLGFAPATSIDEGVPRFVEWYRAYHGV"
     gene            complement(2450..>2816)
                     /locus_tag="VGB57_08415"
     CDS             complement(2450..>2816)
                     /locus_tag="VGB57_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131416.1"
                     /translation="DAYRHLAPHLPARSVRPGGDGKVRLMLGGVSSALPLSAIYVLEP
                     PRSGGESSVERLSGPSAVGALAEHLYGSRWIRPVGDEDLAFCARLARKVPVFALSRPW
                     ALAGLLETADLVRSRAPAG"
BASE COUNT          479 a          997 c          899 g          441 t
ORIGIN      
        1 ctgcgacaaa gcggcggcca tctgctgacg cagttcctgc gtcgtacgac cctgcgcctg
       61 gaccgagccg agcagaggat aatcgatctt gccggcggca tcgaccttgt agtcgcccga
      121 gagcgactcg acctggaaga ccgaaacgct gatcgtgtcg agcgcgccga tcgggtgcga
      181 ggcattggcc accacctgcc cctgcgtgtc gggtgcctgg aagcccgtcg gctcgtaggg
      241 aacggagccg ccgcggctgg cgcagcccgc gaccgtcaga gcggcggcga ccaccagcag
      301 cgcgcgggcc atcagcccac tgagggaaaa gttatgctct tcatgctgaa acgctcgcat
      361 gttcatcttt ctccggtcgg gggcgacaag ccctctacac aatccatggc ttccgtggaa
      421 cagcccatgc gccgattcag cgcacacccc tcctcgcgga aacggagccc gcgccgcccc
      481 tcaaggcgag ctgcctttat ctcgttgcgc cgcttagctt atttctccac cgccgtccag
      541 agtccgacgt tttcccgggc taaaagcttg caactgcggc gaaacaccaa atatcggcgc
      601 ccctaacccc gctggagttg acgtcagtgt tgagtcgcct gttttcgaag agccggacgt
      661 ccagggggcg aagcggcccg cccggcagcc gcgcttatgc gatcggcgac gtccacggcc
      721 ggctcgacct cctcgtcgac ctgctcgccc ggatcgaaga ggacaatgcg cggcgcgggc
      781 ccgccaagac ctggctggtc ttcctcggcg acctggtcga ccgaggtccc gacagccgcg
      841 gcgtcgtcga cctcctcgtc agccgcccgc ccggtttcgc ccgcaacgtc ttcctcaagg
      901 gcaatcatga ggaattcttc ctcggagtgc tcggcggaga cgattcgagc gtccagcact
      961 ggctcgccta cggcggcacc gaatgcgccc agagctacgg cctcaccacc ggctggatgc
     1021 tgaacgcgac tccggccgcg atcatggagc ggctgatccg gcaggtgccc gaaagccacg
     1081 tccgctttct cgacgagatg cacgacagct tccgcttcgg cgactatctg ttcgtccatg
     1141 ccgggatccg tccgggggtg gccctcgaca agcaggtcag ccgcgacctt cgctggatcc
     1201 gggagggttt cctggacgac cggagcgatc atggcgtcgt cgtcgtccac ggccacacga
     1261 tcgtcgagcg ggccgaggag catcccaacc gcatcgccgt cgataccggc gcctatcgct
     1321 cgggcaccct gaccgcgatc ggccttgagg gcgaggagcg ctggttcatc gaggcgcgcg
     1381 agacgggggc cgccgccgcg gcttgagtgg cttggcaggg tggcggcccg ccgataaggc
     1441 cccagccatg aaaatcctcg tgaccggcgc ggccggattc atcggccatc acctcggcgc
     1501 tcggcttctc gcccgcggcg acgaggtggt cggtatcgac aatttcaacg attattacag
     1561 cgtcgccctg aagcgcgacc gcgaggcgaa gcttcgccgc gactttggcg gcttcgacct
     1621 cctcgaggtc gacttcgccg acgaggcggc tttggaccgg gcgctcgacg ggatcgagat
     1681 cgaccggatc gtccacctcg gtgcccaggc cggagtccgc tattcgatcg aaaatccccg
     1741 cgcttacgtc cagtccaacc tggtcggcca cgtcaacatg ctggagatcg cccgccaccg
     1801 cggcctctcg cacatggtct acgcctcctc ctcctcggtc tacggcggca acgacacatt
     1861 gcccttccgg gtcgaggacc gggtcgatca cccgctctcg ctctacgccg ccaccaagaa
     1921 agcggacgag ctgatgagcg agacctacgc ccatctctac cggctgccgc agacgggcct
     1981 cagattcttc accgtctacg gcccctgggg acggccggac atggcgatgt ggctgttcac
     2041 caaagcgatc ttcgccggcg agccgatcca ggtcttcggc gagggcaata tgcggcgcga
     2101 cttcacctat gtcgacgaca tcgtcaccgg ggtcgtcgcc gccctcgaca acccgccgcc
     2161 cgacgacggc gagatcaagg cgggcggcag ccggggcccc caccgcctct acaatatcgg
     2221 caatcaccgc tccgaggagt tgacccgtat gatcggcctg atcgagcagg cctgcggccg
     2281 cgaggcggag aagatcctga tgccgatgca gccgggcgac gtgcgcgaca cctatgccga
     2341 cataagcgcg atccagaacg acctcggctt cgcgcccgcg acctcgatcg acgaaggcgt
     2401 tccgcgcttc gtcgagtggt acagggccta tcacggcgtc tgatccgcgc taccctgccg
     2461 gggcgcggct ccgaacgagg tccgccgttt ccagcaggcc ggccagcgcc cagggcctcg
     2521 acaaagcgaa gaccggaacc ttgcgggcga gccgggcgca gaaggcgaga tcctcgtcgc
     2581 cgacgggacg gatccagcgg cttccgtaga gatgctcggc gagggcgccg acggccgacg
     2641 gccccgaaag ccgctccacc gaggactctc cgccgctccg gggcggctcg agcacgtaga
     2701 tcgccgaaag cggcaacgcc gaggagactc cgcccagcat cagccggacc ttgccgtcgc
     2761 cgcccgggcg cacggaccgg gcgggcagat gcggcgccag atgccggtag gcgtcg
//
LOCUS       DASWNU010000428         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_379658, whole genome shotgun sequence.
ACCESSION   DASWNU010000428 DASWNU010000000
VERSION     DASWNU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379658"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VGB57_08420"
     CDS             <1..686
                     /locus_tag="VGB57_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0131417.1"
                     /translation="KPEYEGWIEAAGYVKAKDLFTYDLEVTAPFPPIVERIVQAGEKN
                     PRIRIRRTVKKQFEAEARLLLGILNDAWSDNWGFVPLTEAEIAYAGRKLKPIVLEDMI
                     LVAEYDGEPVAFMMGLPDINQFLRDLDGSLFPFGWAKLLWRLRRMRPAGGRVPLMGVV
                     KRLHATRLASQLAFMMIEQIRQAGTGHYGISRAEVGWILEDNGPMISIAQAIESDVNR
                     IYRIYEKPL"
     gene            complement(868..1146)
                     /locus_tag="VGB57_08425"
     CDS             complement(868..1146)
                     /locus_tag="VGB57_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0131418.1"
                     /translation="MINLIIALVIGGIIGWLASIVMRTNAQQGILLNIVVGIVGSLLG
                     SFLLGRFFGAEGSIMSGNALDGGTLLAAFIGALILLAIVNLFRRGSVR"
     gene            complement(1247..1870)
                     /locus_tag="VGB57_08430"
     CDS             complement(1247..1870)
                     /locus_tag="VGB57_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY0131419.1"
                     /translation="MGIAAEDLRNSLDALAGREPRIAAALAAAGYPEPRISEPGYQTL
                     LRAIVGQQVSVASAASLWAKLTAAVGDPPDTEVLLAATDETHPGAGQSRQKASYARSL
                     AEEVVSGRLDFAKLPADDEEAIAELVRVKGIGRWTAEVYLLFAEGRTDVWPAGDLAVQ
                     IETGRIMGLDGKPSENRVRELAEAWRPHRGAFAIFAWHHRRNSAAPV"
     gene            1918..2244
                     /locus_tag="VGB57_08435"
     CDS             1918..2244
                     /locus_tag="VGB57_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0131420.1"
                     /translation="MAKLTIINRAGAEKTIEGKDGWSVMENIRDSGFDELLALCGGCC
                     SCATCHVHVDPEWLPALPPMGVDEDDLLDTTDHRVESSRLSCQLKFGPELDGLRVQIA
                     PDDSPF"
     gene            complement(2284..3042)
                     /locus_tag="VGB57_08440"
     CDS             complement(2284..3042)
                     /locus_tag="VGB57_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131421.1"
                     /translation="MSFMRFAFVAAAALTLSSCILSPGKFVSTLHIARDRSFTFTYVG
                     EVILLDPASAMQQGMQDGLAGAEPPVDGEMGDDEVGMIGNTVGTLEGTGNMVGPPAAP
                     AAETAQKIAEAKAIAEALSKEVGYRSVEYLGKNKFRVDYSMTGKLDRSFVYPVNMDAK
                     SIIPWLVVEVRKDRTARFQAIAFGDQDMNMGSAAKPDAGPRERDGTFTFTTDAELVMQ
                     NNEEGMAPGPGKKVVWRVTPTSDTVPTAVVRFAM"
     gene            complement(3047..>3583)
                     /locus_tag="VGB57_08445"
     CDS             complement(3047..>3583)
                     /locus_tag="VGB57_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0131422.1"
                     /translation="QGLVAEVERLDDLLLADILQDAPERRPLLVLDQVTDPHNVGAIL
                     RSAVAFDALALVTQDRHAPPESGALAKAASGALETVPWVRVVNLARALDEMAEAGFWR
                     IGLTGDSEMTLAEAVGPARVALVLGAEGEGMRQNTEAHCDALARLPISGRMESLNVSN
                     AAAVALYAVATHGGNSGG"
BASE COUNT          591 a         1250 c         1167 g          575 t
ORIGIN      
        1 acaagccgga atatgaaggc tggatcgagg ccgccggcta cgtcaaggcc aaggacctct
       61 tcacctacga cctggaggtc accgccccct tcccgcctat cgtcgagcgc atcgtccagg
      121 ccggcgagaa gaacccgcgg atccggatcc gccggaccgt caagaagcag ttcgaggccg
      181 aagcccgcct gctgctcggg atcctcaacg acgcatggtc cgacaattgg ggcttcgtcc
      241 cgctcaccga ggccgagatc gcctatgccg ggcgcaagct gaagccgatc gttctggagg
      301 acatgatcct ggtcgccgaa tatgacggcg agcccgtcgc cttcatgatg ggtctgcccg
      361 acatcaacca gttcctccgc gacctcgacg gaagcctctt ccccttcggc tgggcgaaat
      421 tgctgtggcg gcttcggcgg atgcggccgg cgggaggccg ggtgccgctg atgggcgtgg
      481 tcaagcgcct ccacgccacc cgcctcgcca gccagctcgc cttcatgatg atcgagcaga
      541 tacggcaggc cgggaccggc cattacggaa tcagccgggc cgaggtcggc tggatcctcg
      601 aggataacgg cccgatgatc tcgatcgccc aggcgatcga aagcgacgtc aaccggatct
      661 accggatcta cgagaagccg ctctaaagcg aggcgccccc gtcatcccgg cgaaagccgg
      721 gacctcggga caagaagtca cggcgggtct cgccgaggtt ccggctttcg ccggaatgac
      781 ggggacttgc cgattttccc cgccgcacgc gaaaaggccc gcgggatcgc tcccgcgggc
      841 cttcgctctc aagccgtcag gcccgcttca gcggacgctg ccccggcgga agaggttgac
      901 gatggcgaga aggatgaggg caccgatgaa ggcggcgaga agggtgccgc cgtcgagggc
      961 attgccgctc atgatgctgc cctcggcgcc gaaaaagcgc ccgagcagga agctgccgag
     1021 cagcgagccg acgattccga cgacgatgtt gagaaggatg ccctgctgcg cattggtgcg
     1081 catgacgatg ctggccagcc agccgataat accgccgatg acgagagcga tgatgagatt
     1141 gatcatgagg ctaactccta ctgaactccc ggcccctgtt ttggcccgcg gatcgcagtc
     1201 tagacgcgcc gccgaaaggg atgttccgtg tccggttcga gatgtctcac accggcgccg
     1261 ccgaattgcg cctgtggtgc caggcgaaga tggcgaaggc gccgcggtgg gggcgccagg
     1321 cttcggcgag ctcgcgcacc cgattttcgc tcggtttgcc gtcgaggccc atgatccgcc
     1381 cggtctcgat ctggacggcg aggtcgccgg ccggccagac gtcggtgcgc ccttccgcga
     1441 agagcagata gacctccgcc gtccagcgcc cgatcccctt cacccgcacc agctcggcga
     1501 tcgcttcctc gtcgtcggcc ggcagcttcg cgaaatcgag ccgccccgac accacctcct
     1561 cggcgaggct ccgggcatag gaggccttct gccgcgactg tccggccccc gggtgcgttt
     1621 cgtccgttgc ggcgagcagg acctcggtat cgggcggatc gccgaccgcg gcggtgagct
     1681 tcgcccacag gctcgccgcc gacgccaccg acacctgctg gccgacgatc gcccgaagca
     1741 gggtctggta gcccggctcg ctgatccgcg gctccggata gccggccgcc gccagagcgg
     1801 cggcgatcct cggctcgcgc ccggcaagcg cgtccagcga attgcgcaag tcttcggccg
     1861 cgatccccac ttttgctccc ttgtcttcgc gccccgcgcc tagcatagcc ccggcccatg
     1921 gcgaagctca ccatcatcaa ccgcgccgga gcggaaaaga ccatcgaggg caaggacggc
     1981 tggagcgtca tggagaatat ccgcgactcc ggcttcgacg aattgctcgc tctgtgcggc
     2041 ggctgctgca gctgcgccac ctgccatgtc catgtcgatc cggaatggct gccggctttg
     2101 ccgccgatgg gcgtggacga ggacgacctg ctcgacacca ccgaccaccg ggtcgagagc
     2161 agccgcctct cctgccagct caagttcggt ccggaactcg acggcctcag ggtccagatc
     2221 gcccccgacg attcaccctt ctgagcgatc tgcctccctg ccgcgaagcg gtgggacggc
     2281 agatcacatc gcgaaccgca ccacggcggt cggcacggtg tcgctggtcg gagtgacccg
     2341 ccagaccacc ttcttcccag gtccgggcgc catgccttcc tcgttattct gcatgaccag
     2401 ctcggcgtcg gtggtgaagg tgaaggtgcc gtcgcgctcc ctgggcccgg catcgggctt
     2461 ggcggcgctg cccatgttca tgtcctggtc gccgaaggcg atcgcctgga agcgcgcggt
     2521 gcggtccttg cggacctcga ccaccagcca ggggatgatc gacttggcgt ccatgttgac
     2581 cggatagacg aagcttcggt cgagcttgcc ggtcatcgaa taatcgaccc ggaacttgtt
     2641 cttgccgaga tattcgacgg agcggtagcc gacctccttc gacaaggctt cggcgatggc
     2701 cttggcttca gcgatcttct gggcggtctc ggcggccggc gcggcgggcg ggccgaccat
     2761 gttcccggta ccttcgaggg taccgacggt gttgccgatc atgccgacct cgtcgtcgcc
     2821 catctcgccg tcgaccgggg gctccgctcc ggcaaggccg tcctgcatcc cctgctgcat
     2881 cgccgacgcc ggatcgagca ggatgacctc gccgacatag gtgaaggtga agctccggtc
     2941 cctggcgatg tggagcgtcg agacgaactt gcccgggctc aatatgcagg acgaaagcgt
     3001 gagcgcggcc gcggccacga acgcgaagcg catgaacgac atggaattac ccccccgaat
     3061 tgcccccgtg tgtcgccacc gcgtatagcg cgaccgcggc ggcgttggaa acgttgaggc
     3121 tttccatccg gccgctgatc ggaagccggg ccagagcgtc gcaatgcgcc tcggtattct
     3181 gccgcatccc ctccccttcg gcgccgagca ccagcgccac ccgcgccggc ccgaccgcct
     3241 cggccagggt catctcggaa tcgccggtga ggccgatccg ccagaagccc gcctcggcca
     3301 tctcgtcgag ggcccgcgcc agattgacca cccgcaccca gggcaccgtc tccagggccc
     3361 cgctcgccgc cttggccaga gcccccgatt cgggcggagc gtgccggtcc tgggtgacca
     3421 aagcgagggc gtcgaacgcc accgccgagc gcaggatcgc cccgacattg tgcgggtcgg
     3481 tgacctggtc caggacgagc agcggccggc gctccggggc gtcctggaga atgtcggcga
     3541 gcagcaggtc gtccagccgt tccacctcgg cgaccagccc ctg
//
LOCUS       DASWNU010000429         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_405665, whole genome shotgun sequence.
ACCESSION   DASWNU010000429 DASWNU010000000
VERSION     DASWNU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405665"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VGB57_08450"
     CDS             <1..1196
                     /locus_tag="VGB57_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY0131423.1"
                     /translation="FAAGADSPLLADFRKKVGALDAPADVKARLIADASAALSGPFRR
                     AVGEMLATLDAIEPKSTGNSGAWSLPNGAEYYASRLRAQTTTDLSADAIHQIGLDEVA
                     RIHREMEAVKAQVGFKGTLQAFFQHIKTGAQFKYPNTDAGRQQYLKEANAFIAQVMAK
                     SPQWFSRLPRAPLEVRAVETWRQETASVAFYNRPSPDGSRPGIFYVNLADMNQVLKPQ
                     IEGIAYHEGAPGHHFQIALQQELEGVPKFRRFGGYGAYSEGWGLYAERLGREMGFYQD
                     PYSLFGHLSLELWRAVRLVTDTGLHSKRWTREQAIRYFQDNSLLSERDIVKEVERYIN
                     NPGQATSYKIGQLKIVELRERARTALGERFDIRDFHAAVLENGPLPLDILEQQVEAYI
                     AAKKG"
BASE COUNT          293 a          515 c          511 g          234 t
ORIGIN      
        1 cgttcgcggc gggggcggat tcgcctctgc tcgcggactt ccgcaagaag gtgggtgccc
       61 tcgacgcccc ggccgacgtc aaggcgcggc tgatcgccga cgcctcggcg gcgctttccg
      121 ggccgttccg ccgggcggtc ggcgagatgc tggcgactct cgatgcgatc gagccgaagt
      181 cgaccggcaa cagcggcgcc tggagccttc ccaacggggc cgaatattat gcctcgaggc
      241 ttcgcgccca gaccaccacc gacctctccg ccgacgcgat ccaccagatc gggctcgacg
      301 aggtggcccg gatccaccgc gagatggagg cggtgaaggc gcaggtcggg ttcaagggca
      361 cgctccaggc cttcttccag cacatcaaga ccggcgcgca gttcaaatat ccgaacaccg
      421 acgccgggcg gcagcaatat ctgaaggagg ccaacgcctt catcgcccag gtgatggcga
      481 agtcgcccca gtggttcagc cgcctgccca gggcgccgct cgaggtccgc gcggtggaga
      541 cgtggcggca ggagacggcc tcggtcgcct tctacaaccg gccttcgccc gacggctcga
      601 ggccgggcat cttctacgtc aacctcgccg acatgaacca ggtcctgaag ccccagatcg
      661 agggcatcgc ctatcatgag ggagcgcccg gccatcattt ccagatcgcg ctccagcagg
      721 agctggaggg cgttcccaag ttccgccgct tcggcggcta cggcgcctac agcgagggct
      781 ggggcctcta tgccgagcgg ctgggccggg agatgggctt ctaccaggac ccctattcgt
      841 tgttcgggca cctctcgctc gagctgtggc gcgccgtgcg tctcgtcacc gacaccggcc
      901 tccattccaa gcgctggacc cgcgagcagg cgatccgtta tttccaggac aattcgctcc
      961 tgtccgagcg cgacatcgtc aaggaggtcg agcgctacat caacaatccc ggccaggcga
     1021 cgagctacaa gatcggccag ctcaagatcg tcgagcttcg cgagcgggcc aggacggcgc
     1081 tcggcgagcg cttcgacatt cgcgatttcc acgccgcggt gctcgagaac ggcccgctgc
     1141 cgctcgacat attggagcag caggtcgagg cctatatcgc ggcgaagaag gggtgaccga
     1201 gcggaaccac tccgtcattg cgagcggagc gaagcaatcc agcgcggcgt ttgtcgcacg
     1261 cgccgaagct ggattgcttc gtcgcttcgc tcctcgcaat gacgggcttt aaggagagac
     1321 ggacatggcc aagggtcaga agaagagcaa caaggaagcc aggaagccga agaaggaggt
     1381 caagaagacc atcgccgcca acccttcgag caagggcgtg gtggcgaagg actgatggcc
     1441 gagcccttcg cccgccaccg ccagccctgg aagcaggagg agatccagaa gctccacctg
     1501 ctcgccggca agggcatgtc gctgaaggcg gtcgccaagg cattgacccg gac
//
LOCUS       DASWNU010000430         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_16981, whole genome shotgun sequence.
ACCESSION   DASWNU010000430 DASWNU010000000
VERSION     DASWNU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16981"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VGB57_08455"
     CDS             complement(<1..1576)
                     /locus_tag="VGB57_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131424.1"
                     /translation="MVFTREVARDEKIFTNGGDDLIGVVNGADEADGGSGFDTLFVNY
                     VDATGPVVSGALSGNLTDGYSGIVTSPGRSVAYSGIERLQLIGGAFNDDLRGGAGIDT
                     FNGNGGADTMAGAGGNDIYFVENAGDVVVELAGEGTDEIRTFLAVQNLAGTNVENMRA
                     MSDIAHEFRGNESNNIVTGAAGRDTVYLQQGGNDRVSGLAGNDTFFYGATFTTADTVL
                     GGDGIDGLILQGAYAMTLGTGTTSNITGVETISLAPGHFTDWGDTAGQFYDYSFTTLN
                     GNVAAGAVLKFNGFYLRAGEDFTFNGAAETDGKFILLAGQGVDNLTGGNGNDIFAFGP
                     DGRFGSTDVANGGAGYDSVYLRGDYALDFTAASFGTMTSIESITLGSAAEIEYTAGGD
                     GELDYDLVWDDAMLADNGSITVNGSGLGANETMAFDASDEAGGAFRMFAGAAADTLTG
                     GGGGDLLYGGLGADTLRGNSGADTYRYQASAESTAGAGNYDSILGFTHDVDKIDLSVI
                     DANTARAGNDAFVFIGT"
BASE COUNT          268 a          549 c          545 g          302 t
ORIGIN      
        1 cggtgccgat gaagacgaag gcgtcgttgc ccgcccgggc ggtgttcgcg tcgatcacgc
       61 tgaggtcgat cttgtcgaca tcgtgcgtga agccgaggat gctgtcgtaa ttgccggcac
      121 ccgccgtcga ttccgcgctc gcctggtagc gataggtatc cgccccgcta ttgccgcgca
      181 gcgtatcggc gccgaggccc ccgtagagga ggtcgccccc gcctccgccg gtgagggtgt
      241 cggccgccgc gccggcgaac atgcggaagg cgccgccggc ttcgtcggag gcgtcgaacg
      301 ccatcgtttc gttcgcgccg aggccgctgc cgttaacggt gatcgaaccg ttatcggcga
      361 gcatcgcatc gtcccagacg agatcgtaat cgagctcgcc gtcgccgcct gcggtatatt
      421 cgatctcggc tgcagagccc aaagtgatgc tctcgatgct cgtcatcgtc ccgaagctcg
      481 cggccgtgaa atcgagcgca taatcgccgc gcagatagac cgaatcgtaa cccgcgccgc
      541 cgttcgccac atcggtgctt ccgaagcggc cgtctgggcc gaaggcgaag atgtcgttgc
      601 cgttgccgcc ggtgagattg tcgacgccct ggccggcgag aagaatgaac ttgccgtcgg
      661 tctcggccgc gccgttgaag gtgaaatcct cccccgcgcg caaatagaag ccgttgaact
      721 tcagcacggc tccagcggcg acgttgccgt tcagagtcgt gaagctgtag tcgtagaact
      781 gcccggccgt gtcgccccag tcggtgaagt ggcccggcgc cagcgagatc gtctcgacgc
      841 cggtgatgtt gctggtggtg cccgtgccga gggtcatggc ataggcgccc tgcaggatca
      901 gcccgtctat gccgtcgccg ccgaggaccg tgtcggcggt ggtgaaggtg gcgccgtaga
      961 aaaaggtgtc gttgccggcg aggccgctca cccggtcgtt cccgccctgc tgaagataga
     1021 cggtgtcccg acccgccgcg ccggtgacga tgttgttgct ctcgttgccg cgaaactcgt
     1081 gagcgatgtc cgacatcgcg cgcatattct cgacattggt gccggccagg ttctggacgg
     1141 caaggaaggt gcggatttcg tccgtcccct cccccgcgag ctcgacgacg acgtcgccgg
     1201 cattctcgac gaaatagatg tcgtttccgc cggcgccggc catggtgtcg gcgccgccat
     1261 tgccgttgaa cgtgtcgatg ccggcgccgc cgcgcaggtc gtcgttgaac gccccgccga
     1321 tcagctgcag gcgctcgatg ccgctatagg cgacgctccg gccggggctg gtgactatgc
     1381 cgctatagcc gtcggtcaaa ttgcccgaca aagcgcccga gacgaccggg cccgtcgcgt
     1441 cgacataatt gacgaacaat gtgtcgaacc cgctaccgcc atcggcctcg tcggccccgt
     1501 tgacgacgcc gatcaagtcg tcgcccccgt tggtgaagat cttctcgtcg cgcgcgacct
     1561 cccgtgtgaa gaccacatcc gctccggaac cagtccggag cgtgcccaca tattcgaagt
     1621 ttcggaaccg gtccgccgga aggagaggca gccgcaggtc cgcg
//
LOCUS       DASWNU010000431         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_146057, whole genome shotgun sequence.
ACCESSION   DASWNU010000431 DASWNU010000000
VERSION     DASWNU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146057"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2381)
                     /locus_tag="VGB57_08460"
     CDS             complement(<1..>2381)
                     /locus_tag="VGB57_08460"
                     /inference="COORDINATES: protein motif:HMM:NF045168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131425.1"
                     /translation="SATPHDGHDALVPGHPLHDRIAELAGAAGESGIRLLAVETMRRA
                     SSDPGPGRDHFGFLDGLSQPVMVDAPNPAKRDEVRRGEIFRGYVNDRGDPPVRDPLLD
                     NGTFLVIRKLRQRPGLDAFAEAEAKRLRPKHPGITAAWLLAKMMGRDPDGIALTNPSV
                     GTGNDFDYELDKGERCPLQAHIRRTNPRTREHSRPTPRILRRGLSYGPRQGSAEEGDR
                     GIFFMAYNASIAEQFEVIQRWVNGGNSTGVASCQSDPLMGPAVQGDSRTFRFLSDAGK
                     AVRVDMPKPFVELQWGTYLFVPSIDALKALAAGIPAPATRAEAGNPALRKPDGATIVA
                     SLLALAAQGEEGRRAAAAAWKTHLEDFGEKDPGEKARGPAVWAAIRDEYDGAIRVPYP
                     DHENGGFPRDTVLVASKAMVMRVFQNADGHYSMAGQMKRMRQSFGEIFLGEDDTPSYH
                     AVADPVNAEIMKIDEATAFDIGYRNAAARLEQLLAIFCMLTPGSAGQPRSGQLDLRRE
                     FIMHALGRVCHECFDIPDVIPVSIPPEQATYVELGGWAWGPPGAHPTGGERMPRCPGD
                     YLATSRYCFYPDPVPAVQQYGQKHGQALFEAVWSQLTHLRATGGYPKGLISNAIAPVF
                     TDDAEFARTLIGVMTGFLPPADGSLRWTLYDWLEEKTLWRLQQRYVAATGGAYDRARV
                     ELRGPLVRAMQKRPSPDMLWRTASTAHHLGPVHVQPDDKIIIGIVSAMAEDSERGLED
                     VYPVFGGNRRSNPHPLHACPASRFGMGVMMGILAALFDKVRIEALPAPMLVRI"
BASE COUNT          373 a          780 c          834 g          394 t
ORIGIN      
        1 gatcctgacg agcattggcg cgggcagggc ctcgatacgg accttgtcga acagcgccgc
       61 gagtatgccc atcatcaccc ccatcccgaa gcgcgaggcc gggcaggcgt gaagcggatg
      121 cgggttgctt ctgcgatttc cgccgaagac cgggtagacg tcctcgagac cgcgctcgct
      181 gtcctccgcc atggcggaga cgatgccgat gatgatcttg tcatcgggct ggacgtggac
      241 cgggccgaga tggtgcgcgg tcgaggcggt gcgccacagc atgtccggcg aaggccgctt
      301 ctgcatcgcc cggacgagcg gcccgcgcag ttcgacgcgg gcacggtcgt aggcgccgcc
      361 cgtcgcggca acgtagcgct gctgaagccg ccacagcgtt ttctcttcga gccagtcgta
      421 gagcgtccat cgcaggctgc cgtccgcggg cggcaggaaa ccggtcatga caccgatcaa
      481 ggtccgggcg aactcggcgt cgtcggtgaa gaccggcgcg atcgcgttcg agatcaaccc
      541 cttcgggtat ccgcccgtgg cgcggagatg cgtgagctgc gaccacactg cctcgaacag
      601 cgcctgcccg tgcttctgcc catattgctg aaccgcgggc accgggtccg gatagaagca
      661 gtagcgggag gtcgccagat agtcgccggg gcagcgcggc atccgctcgc cccccgtcgg
      721 atgcgcgcct ggtggacccc aggcccaccc gcccagttcg acataggtgg cctgctccgg
      781 cgggatggag acggggatga cgtcggggat gtcgaaacat tcgtgacaga cgcgtccgag
      841 cgcatgcatg atgaactcgc ggcgaaggtc gagctggccg gagcgaggct gccccgcgct
      901 gcccggggtc agcatacaga atatggcgag cagctgttcg aggcgggccg ccgcattgcg
      961 atagccgatg tcgaacgccg tcgcctcgtc gatcttcatg atctcggcgt tcaccgggtc
     1021 cgccaccgcg tgataggagg gggtgtcgtc ctcgccgagg aagatctcgc cgaacgattg
     1081 ccgcatccgc ttcatctgac cggccatcga gtaatggccg tcggcattct ggaagacgcg
     1141 catcaccatc gccttgctcg cgacgaggac ggtgtcgcgc ggaaacccgc cattttcgtg
     1201 atcgggatag gggacgcgga tggcgccgtc atattcgtcc cttattgccg cccaaaccgc
     1261 cggcccgcgc gctttctcgc ccgggtcctt ctcgccgaaa tcctcgagat gcgtcttcca
     1321 ggccgccgcc gccgcccgcc gtccctcttc tccctgggcc gcgagcgcga gcagggaagc
     1381 tacgatcgtc gctccatcgg gcttgcgaag cgccggattg ccggcctcgg ccctggtcgc
     1441 gggcgccggg attccggccg cgagcgcctt gagggcgtcg atcgagggca cgaacagata
     1501 agtgccccat tgcagctcga cgaacggctt cggcatgtcg acgcgcacgg ccttgccggc
     1561 atcggagagg aagcggaagg tgcggctgtc cccttgcacg gcaggcccca tcaggggatc
     1621 gctctggcac gaagcgacgc cggtgctgtt gccgccgttg acccagcgct gaatgacctc
     1681 gaactgctcg gcgatgcttg cattgtaggc catgaagaag atgccgcggt cgccttcctc
     1741 ggcggagccc tgccgggggc cgtaggaaag gccgcggcgg agaattcgcg gcgtcggccg
     1801 gctatgctcc ctggtgcgcg gattggtgcg gcggatatgc gcctgcagcg ggcacctttc
     1861 gcccttgtcc aactcataat cgaagtcgtt gccggtgccg accgacggat tggtcagcgc
     1921 aatcccgtcg gggtcgcggc ccatcatctt ggccagaagc caggccgcgg tgataccggg
     1981 atgcttgggc cggagccgtt tcgcctccgc ctcggcgaag gcatcaaggc ccggcctctg
     2041 gcgcagcttg cgaatgacga gaaacgtccc gttgtcgaga agcgggtctc gcacgggcgg
     2101 atcgccgcgg tcgttgacat agccgcggaa aatctcgccg cgccgtacct cgtcgcgttt
     2161 ggcggggttg ggcgcatcga ccatcaccgg ctggctcagg ccgtcgagga agccgaaatg
     2221 atcgcggccg ggaccggggt ccgagctcgc ccggcgcatg gtctcgacgg cgaggaggcg
     2281 gattcccgat tccccggcgg ccccggcgag ctcggcgatg cgatcgtgga gcgggtgtcc
     2341 ggggaccagg gcatcatggc cgtcgtgcgg cgtggcggag c
//
LOCUS       DASWNU010000432         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_379827, whole genome shotgun sequence.
ACCESSION   DASWNU010000432 DASWNU010000000
VERSION     DASWNU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379827"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VGB57_08465"
     CDS             complement(<1..1372)
                     /locus_tag="VGB57_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0131426.1"
                     /translation="MRPDVLNPLFAEVEVLKGIGPALAKPLKRLGLNRVVDILFHLPV
                     SWIERKKVDVLDMADAGGVVTIVVTPVDYRQGGARSPFRVRATDRPGNYLSLTYFHNP
                     GWAKKQFPLGVPKVVSGRMEMYGQELQMVHPDYVLEPNEALDLPEREPVYPLSEGLTN
                     RRMADLAGQALGRAPELGEWIEASLLAARGWPSWQEALASVHRSAAVGPARQRLAYDE
                     VFANQLALMLVRASSKRRRGVPLKGDGRLRDALKLPYAATGAQRRAVAEIEGDLQQET
                     PMLRLLQGDVGSGKTLVALMAMLTAVEAGTQGALLAPTEILARQHFDTLSRQLAGLPV
                     NVAILTGREKGRARESTLMGLANGSIDILVGTHAIFQQAVVYKRLGLAVVDEQHRFGV
                     AQRMMLSQKAERPPHLLVMTATPIPRTLTLTHYGEMDVSRLDEMPPGRQPVETRVLSL
                     EKLPDVT"
     gene            1695..>1855
                     /locus_tag="VGB57_08470"
     CDS             1695..>1855
                     /locus_tag="VGB57_08470"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY0131427.1"
                     /translation="MKTFAYRLTAIHRRLDEEIRGELRRRWPDSIRLLRLKTLRLAVK
                     DRLHARMRRG"
BASE COUNT          290 a          627 c          636 g          302 t
ORIGIN      
        1 cggtgacgtc cggcagcttc tcgaggctca gaacccgggt ctcgaccggc tggcgacccg
       61 gcggcatctc gtccagccgg ctgacgtcca tctcgccata atgggtgagc gtaagggtgc
      121 gcggaatcgg cgtcgccgtc atgacgagga ggtgcggcgg gcgctcggcc ttttgagaga
      181 gcatcattcg ctgcgcgacg ccgaagcggt gctgctcgtc gacgacggcg aggccgagcc
      241 tcttgtagac gacggcctgc tggaagatgg cgtgagtgcc gacgaggatg tcgatcgacc
      301 cgttggcgag gcccatcagg gtcgattcgc gcgcccgccc cttctcccgc ccggtcagga
      361 tggcgacgtt gaccggcagc ccggccagct ggcgcgaaag agtgtcgaaa tgctggcggg
      421 cgaggatttc ggtcggggcc agcaaagccc cctgggttcc agcctcgacg gcggtgagca
      481 tcgccatcag agcgaccagg gtcttgcccg agccgacgtc gccctggagc agccggagca
      541 tcggcgtctc ctgctggaga tcgccctcga tctcggcgac ggcgcggcgc tgggcgccgg
      601 tggcggcata tgggagcttg agggcgtcgc gaagccggcc gtcgcccttg agcggaactc
      661 cgcggcggcg cttcgacgag gcccggacca gcatcagggc gagctggttg gcgaacacct
      721 cgtcataagc cagccgctgt cgggccggcc cgacggccgc gcttcggtga acgctcgcaa
      781 gcgcctcctg ccatgacggc cagccgcgcg cggcgagcag gctggcctcg atccattcgc
      841 cgagctccgg agcccggccg agggcctgtc cggcgagatc ggccatgcgc cggttggtga
      901 gcccttccga cagcggatag accggctcgc gctcgggcag gtccagcgcc tcgttcggct
      961 cgagcacata gtccggatgg accatctgaa gctcctgccc gtacatctcc atccggccgg
     1021 agacgacctt cgggaccccg agcgggaact gcttcttcgc ccatcccgga ttgtggaagt
     1081 aggtgaggct gagataattg cccgggcggt cggtcgcccg gacccggaac gggcttcgag
     1141 cgccgccctg gcgataatcg accggagtga cgacgatggt gacgaccccg ccggcgtcgg
     1201 ccatgtcgag cacgtcgacc ttcttgcgct cgatccagct caccggaagg tggaagagga
     1261 tgtcgaccac ccggttcagg ccgagccgct tcaagggctt ggcgagcgcc gggccgatgc
     1321 ccttcagcac ctccacttcg gcgaagagcg ggttgaggac gtcggggcgc atgtcgggtt
     1381 gttagcgcgg cgacccactt tcgtcatgcc ggacttgatc cggcatccct gaacacggcc
     1441 gtgcctccag ctcgagccgc cgtgttcatg gattgcggcc ttcgccggaa cgacgggaaa
     1501 atcgtcatct tgatcctgcc tccatcccga actaaatcca cccctgcgga ccgggcccct
     1561 ctggcgggcg tcctcctgga cgaccgtgat gtcggaccgc atcgggctga gcccggtgca
     1621 gttccacggc ccatcgcccc ctgcatcgca agctccgtcc gatttcgatg atcgatcgat
     1681 tttggagaga ggctatgaaa accttcgcct accgcctgac cgccatccac cgccgcctcg
     1741 acgaggagat caggggcgag ctgcgtcggc gctggcccga cagcatcagg ctgcttcggc
     1801 tcaagacgct gcggctggcc gtcaaggacc ggctccacgc ccggatgcgg cgcgg
//
LOCUS       DASWNU010000433         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_250621, whole genome shotgun sequence.
ACCESSION   DASWNU010000433 DASWNU010000000
VERSION     DASWNU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250621"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGB57_08475"
     CDS             complement(<1..488)
                     /locus_tag="VGB57_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0131428.1"
                     /translation="MRNIDHFIGGGAIASGTRHGDVFDPNNGGVQARVRLGTADDLAR
                     AVANAEEALPAWAATNPQRRARVMFKYKELVEKNMEELAHLLSSEHGKVIADAKGDIQ
                     RGLEVIEFACGIPHALKGEYTQGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMFG
                     VA"
     gene            581..1588
                     /locus_tag="VGB57_08480"
     CDS             581..1588
                     /locus_tag="VGB57_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0131429.1"
                     /translation="MKLGSLKGGRDGRLAVVSRDLAWCADATHIAPTLQAALDDWERI
                     APQLELLATDLEHGAIPRERFHEREALAPLPRAYQWADGSAYVNHVQLVRKARGAEMP
                     ESFWTDPLMYQGASDEMLPPRSPIPLADEDWGCDLEAEVVVVTEDVPQGVSREEALGR
                     VRLIGLVNDVSLRNLIPAELAKGFGFLQSKPASALSPVLVTPDELGERWQDGKLHGAL
                     KVDLNGQPFGRADAGVDMTFDFGTLIAHLARTRNLGAGTIVGSGTVSNRDEGGGPGRP
                     IDQGGLGYSCIAEVRTVETILEGKPKTGFLRHGDTVRLWMDDERGHAIFGTIEQQVLP
                     G"
BASE COUNT          287 a          552 c          610 g          272 t
ORIGIN      
        1 atggcgactc cgaacatcca catcgggatc atcgccggga agttgaacgg ggtgatgccg
       61 gcgacgaccc ccaatggctg gcgcatcgaa tagacgtcga tccccggacc ggcgccttgg
      121 gtatattcgc ccttgagggc atgggggatg ccgcaggcga actcgatcac ttcgaggccg
      181 cgctggatgt cgcccttggc gtcggcgatg accttgccat gctcggagga gaggaggtgc
      241 gccagctcct ccatattctt ctcgaccagc tccttatatt tgaacatcac gcgggcgcgg
      301 cgctgcgggt tggtcgccgc ccaggcgggg agcgcttcct cggcgttggc gacggcgcga
      361 gcgaggtcgt cggccgtgcc gaggcggacg cgagcctgca ctccgccatt gttcggatcg
      421 aacacgtcgc cgtggcgcgt gccggacgca atggcgccgc cgccgatgaa atggtcgata
      481 ttgcgcatgg aaaatcgcct ctgggtctgt ctgccgggat tgacgctggc cctagccaag
      541 ccgcagggca aattccagtt gtgacttggc cggagagagc atgaaactcg gttccttgaa
      601 gggcggccgc gacgggcggc tggcggtggt gagccgggac ctcgcctggt gcgcggacgc
      661 gactcatatc gcgccgactc tgcaggcggc cctcgacgat tgggagcgga tcgcgcccca
      721 gctcgagctg ctggccaccg atctcgagca tggcgccatc ccgcgcgagc gcttccacga
      781 gcgcgaggcg cttgcgcctt tgccgcgcgc ctatcagtgg gcggacggct cggcctatgt
      841 gaaccacgtc cagctcgtcc ggaaggcgcg gggcgcggag atgccggaga gcttctggac
      901 cgatcctttg atgtatcaag gcgcatcgga cgagatgctg ccgccgcgaa gcccgatccc
      961 gctcgccgac gaagattggg gctgcgacct ggaggcggaa gtagtcgtgg tcacggaaga
     1021 cgttccccaa ggcgtctcgc gcgaggaggc gctggggcgc gtccggctga tcggcctggt
     1081 caacgacgtg tcgctgagga acctgatccc ggccgagctc gccaagggct tcggcttcct
     1141 ccagtccaag cccgcatcgg cgctgtcgcc ggtgctggtg acgcccgacg agctcggcga
     1201 gcgctggcag gacggtaagc tccatggagc gctgaaggtg gatctcaacg gccagccctt
     1261 cggccgcgcc gacgcgggcg tcgacatgac cttcgatttc ggtactctga tcgcccacct
     1321 cgccaggacc cggaacctcg gggccggcac gatcgtcggc tccggcaccg tatccaaccg
     1381 cgacgagggc ggcggcccgg gcaggccgat cgaccaggga gggctcggct atagctgcat
     1441 cgccgaggtg cgaacggtcg agacgatcct cgaaggcaag ccgaagaccg gcttcctccg
     1501 ccacggcgac accgtccgct tatggatgga cgacgagcgg gggcatgcga tattcggcac
     1561 gatcgagcag caggtgctcc ccggctgacg ggggccgcgc cccggcgccg cctccattgg
     1621 agcgaagggc gggcccgccc ggccctattc gaaatgggaa aaacgtggat cggcaaaaga
     1681 tcgaggcgcg agtccgggaa ttatcggcca gcggcggcga a
//
LOCUS       DASWNU010000434         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_94871, whole genome shotgun sequence.
ACCESSION   DASWNU010000434 DASWNU010000000
VERSION     DASWNU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94871"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VGB57_08485"
     CDS             complement(<1..539)
                     /locus_tag="VGB57_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY0131430.1"
                     /translation="MILAARLKPGETLGDRIMKVDHAGEHGGICIYRAQILLARWRAP
                     EMVGELEGFLAHERRHRLRFGAELSRRGARRCRSYFLCGLGGFVLGAATGLAGRQAIA
                     ATTVAIERVVIRHMHEQAEALHGLDAQAVAALRDVIADEQSHHDLSEARLDARGPWPK
                     LINPIVAASTEAVIWLGLRV"
     gene            1104..1493
                     /locus_tag="VGB57_08490"
     CDS             1104..1493
                     /locus_tag="VGB57_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131431.1"
                     /translation="MRKSTIRTALASAAILALSAPAIAQEDLAERANELAEQANDVQA
                     QAGALANEASSAAAEREGRNDRDGADGAAADAANATGVGDGHDDRDDGDSGKWGLLGL
                     LGLAGLLGLRKRPDHHVRVDPLRDNRP"
     gene            1942..2832
                     /locus_tag="VGB57_08495"
     CDS             1942..2832
                     /locus_tag="VGB57_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0131432.1"
                     /translation="MSSPPAAAPPHSLRGAALFLLGLLLFACMDTTTKYLAERYDVPL
                     IVAVRYIVNCLLMVVLLAPVHGRRLVETRRTHMVVARALCLAAASLLVGLALQRMPVA
                     ETTAITFLAPMLVVLMAGPLLGERIGAIGWAAALGGFAGVLLVVRPGTGLESAGIAFA
                     LAAVGANAGYQTLSRLLASTERTLALLFYTALIGAIGFGIAAPFFWGGPAPDAFRILL
                     FLSLGVYGGLGHFLFTAAYRHAPASILAPMTYFQLVWAALLGWLVFGHVPDGASAAGM
                     AVIAASGLAIALKPRDPEPA"
     gene            complement(2920..>3844)
                     /locus_tag="VGB57_08500"
     CDS             complement(2920..>3844)
                     /locus_tag="VGB57_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY0131433.1"
                     /translation="HSGAQGFVHSARAAEKLRGPEHHFAWCDELAKWKQGDSTWDNLQ
                     MGMRLGERPRLIVTTTPRATALVRRVKELEGTVETIGRTAENVHSAEAFRQWAAETYG
                     GSRLGRQELDGELFEEVDGALWTRDTIEKARLEGAPPRWKRIVVGVDPPVTAEGDACG
                     IVVCALGENDVACVLADCSVAGERPEGWARAVARSAEAWGADRVVAEKNQGGEMVESV
                     MRNAGVGLPVKLVHAKGSKAARAEPVAALFETGRAKLAGRFPELEDELAGLTHGGGYQ
                     GPGRSPDRADAMVWAMSELVRPRPEPRIRSF"
BASE COUNT          535 a         1512 c         1208 g          589 t
ORIGIN      
        1 acccgcaggc cgagccagat cactgcttcg gtggaagccg cgacgatcgg gttgatcagc
       61 ttcggccaag ggccgcgcgc atcgagccgc gcctcggaaa ggtcgtgatg cgactgctcg
      121 tcggcgatga cgtcgcgaag cgcggccacc gcctgcgcat ccagtccgtg gagcgcctcg
      181 gcctgctcgt gcatgtggcg aataaccacc cgctcgatcg cgaccgtggt cgccgcgatc
      241 gcctggcgcc cggccagccc tgtcgccgct ccgagaacga agccgcccag cccgcacagg
      301 aagtagctcc ggcaccgcct cgcgccgcgg cggctcagct ccgccccgaa gcgcagccgg
      361 tgccggcgct catgggccaa gaagccctcg agttcaccga ccatttcggg cgcccgccac
      421 cgcgccagca ggatctgcgc acggtatatg cagatcccac cgtgctcgcc ggcatggtcg
      481 accttcatga tccggtcgcc aagcgtctct ccgggcttca gtcgggcggc cagaatcatc
      541 ctcgccgtcc tagaccggcc gcgccggcag gcaaatcgct tcctccctcc gcgctcgatg
      601 tccgcttcgg ccagtcgccg gcgactgccc ccctcctgga gccagacgtc gggccggacc
      661 gccggccgtt gccgggtccc ggtcgggcaa cgcataacga ccggtcgcaa atcggcggcc
      721 tctgccggca gacggtcgtg acacaggagg cgttcgccca ttggtcgctc gctctcctcc
      781 acttggccaa cggccgaagc ggcgacgccg cggccgaata tcggcgggcc gcgcaactcc
      841 acccccgcga atatgcggca acaccgaccg aagctcggct ggccaccgag atagcccaac
      901 cccgcttccg tttccttcgc gacaatcagg ccttccaacg ggccttgccc gcattcctgg
      961 cggccctggc cgctccggcg cctgcgccat ggcgccggga ctggacagcg catcgacctc
     1021 ctttcgcctc cggaacagtc ccggaccttc gcccgttcag gatccgcccg gtgcgtacat
     1081 cgggtgcaac agggagaaga ttcatgcgca aatcgacgat ccggaccgct ctcgcgtccg
     1141 ccgccattct cgccctctcg gcgccggcca tcgctcagga agacctggcc gagcgggcga
     1201 acgagctcgc cgagcaggcc aacgacgtcc aggcccaggc cggagcgctc gccaacgagg
     1261 cttcgagcgc cgcggccgag cgcgaaggtc gtaacgaccg cgacggcgcc gacggggccg
     1321 cggccgatgc cgccaacgcc acgggcgtcg gcgacggcca tgacgaccgc gacgacggcg
     1381 acagcggcaa atggggcctg ctcgggctgc tcggcctcgc gggactgctc ggcctaagga
     1441 agcggcccga ccaccacgtc cgagtcgacc cgctccgcga caatcggccg taaggtcgga
     1501 aaggggggac agtcaccggg atcggtcgga tcggcgactc gtcgaggagg aggggggagt
     1561 cagggccggc acccgtgctt gcggatgccg gccccgcccc cgttacgcaa ctggaggggc
     1621 ccgtccgggc cggcgctcga catacatcgt cgaaaagccg cgccggccgt ggagggcgag
     1681 cgggtctctg cacgattggc gatgatgagc gtctgaacga gcctgacagt cgccgttcac
     1741 ctgatgcccc gagcacggga ggcgcggccg gcgcggccgg ggcgaaaggg ccgggggtcg
     1801 cgtaaatcgc ccccggcccc cgctgccgaa ggaactgaat cctcggtggc tgggcgggcc
     1861 ttaaccctgt tgggcgcgga agcaaagcgc catgttttca acgggaaagg accccgggcg
     1921 cggcttcttt gttatgcaat gatgtccagt cccccggcgg ccgcgccccc tcattcgctt
     1981 cgcggcgcgg cgctcttcct gctcggcctg ctccttttcg cctgcatgga cacgacgacc
     2041 aaatatctgg ccgagcgcta cgacgtgccg ctgatcgtcg ccgtccgcta catcgtcaat
     2101 tgcctgctga tggtcgtgct cctggccccc gtccacggcc gccgcctggt cgagacccgg
     2161 cgcacccata tggtcgtcgc ccgcgccctg tgcctcgccg ccgcgtcgct tctggtcggc
     2221 ctcgcgcttc agcgcatgcc ggtcgccgag accaccgcga tcacctttct cgcgccgatg
     2281 ctcgtcgtcc ttatggcggg gccgctgctg ggcgagcgga tcggggcgat cggctgggcg
     2341 gcggcgctgg gcggcttcgc gggcgtgctg ctcgtcgtcc ggcccggcac cgggctggag
     2401 tcagcgggga tcgccttcgc cctcgccgcg gtcggcgcca acgccggcta tcagacgctc
     2461 tcgaggcttc tcgcctcgac cgagcggacc ctcgccctgc tcttctacac cgccctcatc
     2521 ggagcgatcg gcttcgggat cgccgcgccc ttcttctggg gcggccccgc ccctgacgcc
     2581 ttccggatcc tgctcttcct cagcctcggc gtctacggag gcctcggcca cttcctcttc
     2641 accgcggcct atcgccacgc ccccgcctcg atcctcgccc cgatgaccta tttccagctc
     2701 gtctgggcgg ccctgctggg ctggctggtc ttcggccacg tccccgatgg ggcgagcgcc
     2761 gccggaatgg ccgtgatcgc cgcctccggc ctcgccatcg ccctgaagcc gcgcgacccg
     2821 gagcccgcct agagcctttt cgagcgcact cgaacggcgg cggcctggaa aacgcggctc
     2881 ggaaaatcct ggagcgagcc gacctcggtc gaactcgctc tagaagctcc tgatccgggg
     2941 ctccggccgc ggcctgacga gctcgctcat cgcccacacc atcgcgtcgg cacggtcggg
     3001 cgagcgcccc ggcccctgat agccgccgcc atgggtcagc cccgccagct cgtcctcgag
     3061 ctccggaaag cgccccgcga gcttcgcccg ccccgtctcg aacaaggccg cgaccggctc
     3121 cgcccgggcg gccttgctcc ccttggcatg gaccagcttc accggaagcc cgacgccggc
     3181 gttgcgcatc acgctctcga ccatctcccc gccctggttc ttctcggcca ccacccggtc
     3241 cgcgccccac gcctccgccg accgggccac cgcccgggcc cacccttccg gccgctcccc
     3301 ggcgacgctg caatcggcga gcacgcaggc cacgtcgttc tcgccgagcg cgcagacgac
     3361 gatcccgcag gcatcccctt ccgccgtcac cggcggatcc actccgacga cgatccgctt
     3421 ccaccgcggc ggcgcacctt cgagcctcgc cttctcgatc gtgtcgcggg tccacagcgc
     3481 gccgtcgacc tcctcgaaca gctccccgtc cagctcctgc cgcccgagcc tcgagccgcc
     3541 gtaggtctcc gccgcccact gccggaaagc ctcggccgaa tggacattct ccgccgtccg
     3601 cccgatcgtc tcgaccgtcc cctcgagctc cttcacccgt cggaccagag ccgtcgcccg
     3661 gggcgtcgtg gtgacgatca gcctcggccg ctccccgagc cgcatcccca tctgcagatt
     3721 gtcccaggtc gaatcgccct gtttccattt cgccagctcg tcgcaccagg cgaaatgatg
     3781 ttccggtcct cgcaatttct ccgcagcccg agccgaatgg acgaagcctt gcgctcccga
     3841 atgg
//
LOCUS       DASWNU010000435         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_379892, whole genome shotgun sequence.
ACCESSION   DASWNU010000435 DASWNU010000000
VERSION     DASWNU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379892"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VGB57_08505"
     CDS             <1..320
                     /locus_tag="VGB57_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEY0131434.1"
                     /translation="LGYPTANVALGPYLRPRFGIYAVRGRLGDGRVVGGAANLGIRPT
                     FEPPRELLEPYFFGFDGDLYDRTIEVELISFLRPEARFESLEALAGQMAADCDEARRR
                     LAE"
     gene            317..1285
                     /locus_tag="VGB57_08510"
     CDS             317..1285
                     /locus_tag="VGB57_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY0131435.1"
                     /translation="MKIWRWAGAGIATFAVAVSLINASWIAGRSQKPLILVAHRGLAQ
                     QFDRAGVGNETCTATRIRPSDHDFIENTLRSMSNARHFGADAIELDVHPTRDGQMVVF
                     HDWTLDCRTNGKGPVRKHTLAELKRLDVGYGYTTDGGRTFPLRGKGVGGMPTVEEALR
                     ETPRMRLFFNFKSKDPRDADALVAAFRRAGVEIDGKFAFYGHPRVTARMKQLVPGAWT
                     FWKGDMKACLTDYVKFGWTSFIPKSCHNTTVAVPLNYQWAVWGWPKRFIARMESVGTR
                     VILMGPYENGEIAGIERAEQLGKVPRDFRGYLWIEDFYNVGRALEN"
     gene            1415..>2834
                     /locus_tag="VGB57_08515"
     CDS             1415..>2834
                     /locus_tag="VGB57_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0131436.1"
                     /translation="MSEKPDYRDTVFLPKTDFPMKAGLAAKEPAILARWAEIGLYKRL
                     REARAGRTRFILHDGPPYANGDIHMGHAMNKILKDMVVRSQSLLGKDAPYVPGWDCHG
                     LPIEWKIEEQYRKKKLDKDAVPPQEFRAECRAYAEKWVNIQSEQFQRLGVMGEWDDPY
                     LTMKFEAEAKIVEELLKFAESGQLYRGAKPVMWSPVEKTALAEAEVEYEEIVSTQIDV
                     GFEIVESPIEELVGALAVIWTTTPWTIPVNQAIAYGPEIDYWLARFDHDAGTFTVLVA
                     RDLIESFGGRVGHPVVVKIGTDGGTVTPLKSLKGSELAGTIARHPMHELGGFFARPRP
                     FLPGEFVTTEQGTGLVHMSPDHGEDDFLLCKAHGIDPVFAVDGAGFYRNDWEWLGGLG
                     SVINTKFNAPDGPICTGLREAGGLLAASADFRHSYPHSWRSKAKIIFRCTPQWFIPMD
                     KSPSPSRGEGQRSQATQGEGAEA"
BASE COUNT          522 a          933 c          918 g          461 t
ORIGIN      
        1 agttgggcta tccgaccgcc aacgtcgcgc tcggccccta tctgcgcccg cgcttcggga
       61 tctacgcggt tcgcggccgg ctcggggacg ggcgggtggt gggcggcgcc gccaatctcg
      121 gcatccgccc gaccttcgag cctcccaggg agctgctcga gccctatttc ttcggcttcg
      181 acggcgatct ctacgaccgg acgatcgagg tcgagctgat ctccttcctt cgccccgagg
      241 cgaggttcga aagcctggaa gccctcgccg gccagatggc ggccgattgc gacgaagctc
      301 gaaggaggct cgcggaatga agatctggcg atgggcgggc gccgggatcg cgaccttcgc
      361 ggtcgcggtg tcgctgatca acgcctcgtg gatcgccggg cggtcgcaaa agccgctgat
      421 cctcgtcgcc catcgcggac tcgcccagca attcgaccgg gccggggtcg gcaacgagac
      481 atgcaccgcc acccggatcc gtccgtccga ccatgatttc atcgagaaca cgctccgctc
      541 catgtccaac gcccgccatt tcggcgcgga tgcgatcgag ctcgacgtcc atcccaccag
      601 agacgggcag atggtcgtct tccacgactg gacgctcgac tgccgaacga acggcaaggg
      661 accggtccgc aagcacacgc tcgccgagct caagcggctc gatgtcggct acggctatac
      721 gacggacggc ggcaggacct ttcccctgag gggcaagggc gtcggcggga tgccgacggt
      781 cgaggaagcg ctgcgcgaga ctccgcggat gcggttgttc ttcaacttca agagcaagga
      841 cccgagggac gccgacgccc tcgtcgccgc cttccggcga gccggagtcg agatcgacgg
      901 caagttcgcc ttttacggcc acccccgggt caccgctcga atgaagcagc tggtgcccgg
      961 cgcctggacc ttctggaaag gcgatatgaa ggcgtgcctc accgattacg tgaagttcgg
     1021 ctggacgagc ttcatcccca aaagctgcca caacacgacg gtggcggtgc cgctcaacta
     1081 ccaatgggcg gtctggggct ggcccaagcg cttcatcgcc cggatggaga gcgtcggaac
     1141 cagggtgatc ctgatgggac cttatgagaa tggcgagatc gccggaatcg agcgcgccga
     1201 gcagctcggc aaggtgccga gggacttccg cggctatctg tggatcgagg atttctacaa
     1261 cgtgggacgg gcgctggaga attgaggctt ggcgtcgtcc cggcggaagc cgggacctcg
     1321 gggagacccg ccgagactat ttgtcctgag gtcccggctt tcgccgggat gacgaaactt
     1381 cccattcccc gccttcctcg ctaatccccc gccaatgtcc gaaaaacccg attaccgaga
     1441 cacggtcttc ttgcccaaga ccgacttccc gatgaaagcc ggcctcgcgg cgaaggagcc
     1501 ggccatcctc gcgcgctggg ccgagatcgg gctctacaag cgcctgcgcg aggcgcgcgc
     1561 gggccggacc cgcttcatcc tccacgacgg cccgccctac gccaatggcg acatccacat
     1621 gggccacgcc atgaacaaga tcctcaagga catggtggtt cgttcccagt ccctgctcgg
     1681 caaggacgcg ccctacgtgc ccggctggga ctgccacggc ctccccatcg agtggaagat
     1741 cgaggagcaa taccggaaga agaagctgga taaagatgca gtgcctccgc aggaattccg
     1801 cgcggaatgc agggcttatg cagaaaagtg ggtaaatata cagtccgagc agttccagcg
     1861 gctcggagtc atgggcgagt gggacgaccc ctacctgacc atgaaattcg aggccgaggc
     1921 caagatcgtc gaggagctct tgaagttcgc cgagagcggc cagctctatc gcggcgccaa
     1981 gccggtgatg tggtcgccgg tcgagaagac ggccctggcc gaggccgagg tggaatatga
     2041 ggagatcgtc tcgacccaga tcgatgtcgg tttcgagatc gtcgagagcc cgatcgagga
     2101 gctggtcggc gccttagcgg tcatctggac gacgacgcct tggacgattc cggtaaacca
     2161 ggcgatcgcc tacggccctg aaatcgatta ttggctcgcc cgcttcgacc acgacgccgg
     2221 cactttcacg gtgcttgtcg cacgcgacct gatcgagagc ttcggcggcc gcgtcggcca
     2281 cccggtggtg gtcaagatcg gcaccgacgg cggcaccgta acgccgctca agagcttgaa
     2341 gggctcggag ctcgccggaa cgatcgcccg ccacccgatg cacgagctgg gcggcttctt
     2401 cgcccggccc cgccccttcc tgcccggcga gttcgtcacc accgaacagg gcaccggcct
     2461 cgtccatatg tcgcccgacc atggcgagga cgatttcctg ctctgcaagg cgcacggcat
     2521 cgatcccgtc ttcgcggtcg acggcgccgg cttctaccgg aacgactggg aatggctcgg
     2581 cgggctcggt tcggtgatca acaccaagtt caacgcgccc gacgggccga tctgcaccgg
     2641 ccttcgcgag gcgggcgggt tgctggcggc gtccgccgac ttcaggcaca gctacccgca
     2701 cagctggcgc tccaaggcca agatcatctt ccgctgcact ccccagtggt tcatcccgat
     2761 ggacaaaagt ccctctccct caaggggaga gggacagcgg agccaagcga cacagggtga
     2821 gggcgcggag gccg
//
LOCUS       DASWNU010000436         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_146197, whole genome shotgun sequence.
ACCESSION   DASWNU010000436 DASWNU010000000
VERSION     DASWNU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146197"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGB57_08520"
     CDS             <1..459
                     /locus_tag="VGB57_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0131437.1"
                     /translation="TVDRWEAHMATNLRAPALLSQAFAARARGAGLIVNLLDSKLAQP
                     NPDFFSYTVSKFGLAGLTELCARAWAGRGIRVCAIAPSVTLVSGAQQNFDSVHGLNAL
                     GRGVRVDEIVGALRFIVATPTLTGQTLVLDGGQRFLGLPRDVQFLEPDDE"
     gene            452..853
                     /locus_tag="VGB57_08525"
     CDS             452..853
                     /locus_tag="VGB57_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY0131438.1"
                     /translation="MNEAVKLDGLVPERLAPRSRKIVLDDYCVATDIGFHEFEVGVPQ
                     RLLVTVEVWVDEASFPADDQPGGAWDYDFLRTEIGALIEGRRFNLQETVARAIYDLVA
                     ARRGVTGLRVATRKPDIYPDCAGVGVELTSY"
     gene            complement(858..1715)
                     /locus_tag="VGB57_08530"
     CDS             complement(858..1715)
                     /locus_tag="VGB57_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131439.1"
                     /translation="MAGRLTFYKRPLAAALIAAAAVPAESQAPTPSLTPGLTYADLAD
                     LALAAPVAAHVRVAEAAAVKPERAPGLKPGMARFYVQAGLVSLIRSPQSLPARLSYVV
                     DLPRDSKGKAPKLRRGGEFLLLAAPVPGKPGELQLAAADAQIAFTPERASTLRSIIRE
                     ASSASPPPRIAGIGRAFHVPGAIRGESETQIFLQTAGGTPVSLTILRRPGETPHWAVA
                     LSEIVDEAAEPPAPGTLLWYRLACGLPRALPRQSLAEADAPGAAAIQADYRLVLDRLG
                     PCVRARGRV"
     gene            1973..2653
                     /locus_tag="VGB57_08535"
     CDS             1973..2653
                     /locus_tag="VGB57_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0131440.1"
                     /translation="MAYADNRMSRSRMTAIFIVALLHALLGYALITGLAYNVVKKVAA
                     DLKTFNVEEEPPPPEEKPPPPPPDQPQTPPPVVTPPPIVRTNTPPPPMVTTNVAPPPV
                     ITYTAPPAPRPAPPAPPPPPPPPPKKVTPARAKANLGSLFSDEDYPASAVRSGESGAT
                     GFRLEVGANGRVTNCTVTASSGSSALDSTTCRLLRSRARFTPATDSTGSTTSDSVSGR
                     IVWRLPAD"
     gene            2711..3532
                     /locus_tag="VGB57_08540"
     CDS             2711..3532
                     /locus_tag="VGB57_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0131441.1"
                     /translation="MATPAAGAAHGGENPYGLMAALEQGGAIAWATFIILVIMSAGSW
                     YVLFVKLFEQQKIINQGRRARTSFWQSANLREGASKLEKNSAYRQIVDDGILALEQHD
                     KLTDPIDQHDWMLNSLARSQGLISSRLSSGLAFLATVGSTAPFVGLFGTVVGIYRALV
                     KIGAAGQASIDTVAGPVGEALIMTALGLIVAVPAVMAYNWLMRRNKAVLEELARFSND
                     LHGYMMSGGTVRPAVAAARTGSTGAASASPGAASPGGTTATGSATGTTTGSPKKL"
BASE COUNT          587 a         1247 c         1243 g          569 t
ORIGIN      
        1 accgtcgacc ggtgggaggc gcatatggcg acgaaccttc gggcgccggc cttgctctcg
       61 caggcgttcg ccgcccgggc gcgaggcgcg ggcctgatcg tcaacctgct cgattcgaag
      121 ctggcccagc ccaatcccga tttcttcagc tatacggttt ccaagttcgg gcttgccggc
      181 ctcaccgagc tttgcgcccg ggcctgggcc gggcgaggca taagggtctg cgcgatcgcg
      241 ccctcggtga ccctcgtctc cggcgcccag cagaatttcg actcggtgca cggcctcaac
      301 gccctggggc ggggagtgcg ggtcgatgag atcgtcggag cgctccgctt catcgttgca
      361 acgccgactc tcaccgggca gacgctcgtc ctcgacggcg gccagcgctt tctcggcctc
      421 ccccgcgacg tgcaattcct ggagccggac gatgaatgag gcggtgaagc tcgacggcct
      481 cgtgcccgaa cgactcgcgc cgaggagccg caagatcgtc ctcgacgatt attgcgtcgc
      541 gaccgacatc ggcttccatg agttcgaggt cggggtgccg cagcggcttc tcgtcaccgt
      601 cgaggtctgg gtggacgagg ccagcttccc ggccgacgac cagcccggcg gcgcctggga
      661 ctacgacttc ctgcgcaccg agataggcgc tctgatcgaa ggccgccggt tcaacctgca
      721 ggagacggtg gcccgggcga tctacgatct cgtcgcggct cggcgcgggg tcacgggact
      781 gcgggtggcg acccgcaagc ccgacatcta tccggactgc gccggcgtgg gggtggagct
      841 cacgtcctac tgagggctca gacccgcccc ctcgccctca cgcacggccc cagccgatcg
      901 agcaccagcc ggtaatccgc ctggatcgcc gccgcgcccg gcgcatcggc ctcggcgagg
      961 ctctgcctcg gcagggcgcg cgggaggccg caggccaggc ggtaccagag cagggtgccc
     1021 ggcgccggcg gctcggccgc ctcgtcgacg atctcgctca gggcaacggc ccagtgggga
     1081 gtctcgccgg gccgccgcaa tatggtcagc gacaccgggg tgccgccggc ggtctgaagg
     1141 aagatctgcg tctcgctctc gccgcgaatc gctccgggca cgtggaaggc gcggccgatt
     1201 ccggcaattc gcggcggcgg cgaggcggag ctcgcctcgc ggatgatcga gcgcagggtc
     1261 gaggcgcgct ccggcgtgaa ggcgatctgg gcgtcggcgg cggccagctg aagctcgccc
     1321 ggcttgccgg gcacgggcgc ggccagaagg aggaattcgc cgcccctgcg cagcttgggc
     1381 gccttgccct tcgagtcgcg cggaagatcg acgacgtagc tgagccgcgc cggaagcgac
     1441 tgcggcgaac gtatcagcga gaccagcccc gcctggacgt agaatcgggc catgcccggc
     1501 ttcaggccgg gcgcgcgctc gggcttgacc gcggcggcct cggcgacgcg gacgtgggcg
     1561 gcgaccggcg cagcgagcgc gaggtcggcg agatcggcgt aggtcagacc tggggtaagg
     1621 ctcggggtcg gcgcctgcga ttcggccggg accgccgcgg ccgcgatgag ggccgccgcg
     1681 agcggccgtt tataaaaagt taacctgcct gccactgttc cgcctccgta agtttatacc
     1741 ccgtttccca tatgcttaga gggatgaacg gcggatttcg gccattttag gcgcaagcgc
     1801 cttgcattca caggaggatt gcgtcctcct tagtctcacg atagaggaac ctcctccact
     1861 ggatgatatg ttgatacatc gcatatcgga tggagccgtt ggggatcagc cgcgttgttg
     1921 acgatgcgcg ggtctccggc agagagacga agagagggag tgtggctgat taatggcata
     1981 tgctgacaac aggatgagcc ggagccgcat gacggcgatc ttcatcgtcg ccctgctcca
     2041 cgccctcctt ggctacgcac tcatcaccgg cttggcctat aatgtggtca agaaggtggc
     2101 ggccgatctc aagactttca acgtggagga ggagccgccg ccccccgagg agaagccgcc
     2161 gcccccgccg ccggaccagc cgcagactcc gccgccggtg gtgactccgc cgccgatcgt
     2221 ccggaccaac acgccgccgc cgccgatggt gacgacgaac gtggccccgc cgccggtgat
     2281 cacctacacg gcgcctccgg ctccgcggcc ggcgccgccg gcaccgccgc cgccgccgcc
     2341 gccgccgccg aagaaggtga cgcccgcccg ggcgaaggcg aatctcggtt cgctcttctc
     2401 ggacgaggat tatccggctt cggccgtccg ctccggggaa tcgggcgcga ccggcttccg
     2461 gctcgaggtt ggcgcgaacg gccgggtcac caactgcacg gtgacggcgt cgagcggctc
     2521 gtccgccctg gattcgacca cttgtcgact gttgagatcg cgcgcccgtt tcacgcccgc
     2581 gaccgacagt accggatcga cgacgagcga ctcggtcagc ggccggatcg tctggcgtct
     2641 gccggccgat tgaggacgat cgaccggaac atacgatttt caaaatctat ctgagaggaa
     2701 agtagagacc atggccaccc cagcagcagg cgcagcccac ggcggcgaaa acccctatgg
     2761 cctgatggcc gcactcgagc agggcggagc gatcgcctgg gcgaccttca tcatcctcgt
     2821 gatcatgtcg gccggttcct ggtacgtgct gttcgtcaag ctgttcgagc agcagaagat
     2881 catcaaccag ggccgccgcg cccggaccag cttctggcag tcggcgaacc tgcgcgaggg
     2941 cgcttccaag ctcgagaaga acagcgccta ccgccagatc gtcgacgacg gcatcctcgc
     3001 tctggagcag catgacaagc tgaccgatcc aatcgatcag cacgactgga tgctcaactc
     3061 gctggcgcgc agccagggcc tgatcagctc acgcctgtcg agcggcctcg ccttcctcgc
     3121 caccgtcggc tcgaccgctc cgttcgtcgg cctgttcgga accgtcgtcg gcatctaccg
     3181 cgcgctggtg aagatcggcg ccgccggtca ggcctcgatc gacaccgtcg ccggcccggt
     3241 cggcgaggct ctgatcatga ccgcgctcgg cctgatcgtc gccgttccgg ccgtgatggc
     3301 ctataactgg ctgatgcgcc gcaacaaggc ggtgctcgag gagctcgctc gcttctcgaa
     3361 cgacctccac ggctacatga tgtcgggcgg tacggttcgt ccggcggttg cggcagcgcg
     3421 gaccggatcg accggagctg catcggcgag cccaggagca gcgagccccg gcggcaccac
     3481 tgccaccggc agcgcaactg gcacgaccac cggcagcccg aagaagcttt gagccttcgg
     3541 cggcgggcgg aggctgctcc gcccgccgct cgatggtccc cggttgcaag cagaagatag
     3601 gaaactgata ctagggccat gagcctcggc ccaggggaag gcagcg
//
LOCUS       DASWNU010000437         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_509982, whole genome shotgun sequence.
ACCESSION   DASWNU010000437 DASWNU010000000
VERSION     DASWNU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509982"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VGB57_08545"
     CDS             complement(<1..682)
                     /locus_tag="VGB57_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0131442.1"
                     /translation="MADKDRIDLRPQDHDPLQSSTLRAGPSGDTRESLFSFLDRLSQA
                     ASKSTDADQILTITTSLTAEHLGITNCAYADMDEDEDGFTIRGDWHAPGSPSIVGHYS
                     LADFGALAVRELKAGRPLIINDNLREIAPHEARTFQDIGIAATICMPLVKAGRLSALM
                     AIHDKVPHFWSAYELELIREVTERSWAHVERIRAEAELRASHERFRAAVQATRGVLWT
                     NDAEGRMHG"
     gene            1182..>2612
                     /locus_tag="VGB57_08550"
     CDS             1182..>2612
                     /locus_tag="VGB57_08550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0131443.1"
                     /translation="MAASGAPLFLLSFRHRDELTATAERAGWLAIAARRADNAESRFI
                     ASGASVAVVDARGALPEGKEAVRSLADPAEANCSALLVLLSRGDSDALDSLYADGATH
                     FLVSPFSDSQFLAALQYAHRHAERVGGRRPLRRGEPEGQKGKPGSASWRWTPGSPSIE
                     LSPALARRAGLGEEAGQRISLMELFRKLDPDGRKAARGAIDRLLSTGRSTAFAHTDGE
                     GEGARLAHHVRVEGAKDGGAVVGRTESIVPLEGAAWQSRDPMTGVRDGRAARAWIQRQ
                     LKAEGQGQPALVVLLLAVSRFDTINAAFGRPTGDAVLQAAARRIERLADSDGRRRLVA
                     RIAGAEFAILLAAPTSLGEGRFLAGQLVEAIGRPFMSGDHVITLGSRVGVVASEGDED
                     AASLLRRASAALAEAKSSEAGPVKVVDAGAASDSALGDQLEIDLRRALDQDEIEILFQ
                     PQVSIANRRIIGAEALARWRHPEFGEL"
BASE COUNT          384 a          914 c          906 g          408 t
ORIGIN      
        1 caccgtgcat ccgcccctcg gcatcgttgg tccagagcac tccgcgcgtt gcctggaccg
       61 ccgcccggaa acgctcatgg ctggcgcgca gctcggcttc cgcgcggatc cgctcgacat
      121 gcgcccacga tcgctcggta acctcgcgga tgagttcgag ctcgtaggcg gaccaaaaat
      181 gggggacctt gtcgtggatc gccatcaacg cgctcagccg cccggccttc acgaggggca
      241 tgcagatcgt cgcggcgatg ccgatgtctt ggaacgtcct ggcctcgtgc ggcgcgatct
      301 cgcgcagatt gtcgttgatg atcagcggcc gccccgcctt gagctcccgc acggccagcg
      361 cgccgaagtc cgcgaggctg taatggccca cgatcgaagg ggagcccggc gcgtgccaat
      421 cgccccttat ggtgaagccg tcctcgtcct cgtccatgtc cgcataggcg cagttggtta
      481 tgcccagatg ctcggccgtg aggctggtcg ttatcgtcag gatctggtct gcgtcggtcg
      541 atttggacgc ggcctggctc agccggtcca ggaagctgaa cagcgattcg cgcgtgtctc
      601 cgctggggcc tgctcggaga gtcgagcttt gcagcggatc gtgatcctgg ggccgaaggt
      661 cgatacgatc cttgtcagcc aaatcaccca ctccgcagcc gtaccccgcc ccgtcggcgt
      721 acgatatcaa gcgttgggcc gtctaccggt tcccgaattg ccctggccgc ccctcgcgcc
      781 ggtcgctcag cactgctcgc agataaggcg cgacggctgg cacaaagccg gccggcgccg
      841 ggtcacgtgc ccgcattcga gctgcatggc gacggagcct cggccgcacg gagtgcgtgc
      901 ggtcacggca cggcttcgcc ggtcgcgggg acgcagcagc gtctcctctt cggatatcat
      961 gtcaattccc cctgctttcc gtaaaaacgg ctcgcaatca aagctgtagc aagctttcct
     1021 gccggcacca aggtggtttg tcgccaattt ggaccggagt gactcaatcg ggcccgtccc
     1081 gccccggcac ctcggccgat ctaaaccgat tgttgaggtc aagcgcccag agtagagcga
     1141 tggggttcga agcgccgtcg acggggaagg gagagggagg catggccgcg tccggcgcac
     1201 cgctcttcct cttgtccttc cgccatcggg acgagctcac cgccaccgcc gagcgggccg
     1261 ggtggctggc gatcgcggcc cggcgggcgg acaatgccga aagccgcttc atcgcctcgg
     1321 gagcgtcggt ggcggtggtc gacgcccgcg gcgcccttcc ggagggcaag gaggcggttc
     1381 gaagcctcgc cgatcccgcc gaggccaatt gctcggcctt gctggtcctg ctctcgcgcg
     1441 gcgattccga cgcgctcgac tcgctctatg cggacggcgc cacccatttc ctggtcagcc
     1501 cgttcagcga ctcccagttc cttgcagcgc ttcaatatgc ccaccgccat gccgagcggg
     1561 tcggcggccg ccggccgctt cgccgaggcg agccggaagg gcagaagggc aagccgggaa
     1621 gcgcctcgtg gcgctggacg cccggctcgc cctcgatcga gctgagcccg gcgctggccc
     1681 gccgagccgg cctcggcgag gaagccgggc agcggatcag cctgatggag ctgttccgaa
     1741 agctcgaccc cgacggccgc aaggcggcgc ggggcgcgat cgaccggttg ctctccaccg
     1801 gccggtcgac ggctttcgcc cacaccgacg gggagggcga aggcgcccgt ctcgcccacc
     1861 acgtaagggt cgagggcgcc aaggacgggg gggccgtggt cggccgaacc gagtcgatcg
     1921 tcccgctgga aggcgcggcc tggcagtcgc gcgatccgat gacgggggtg cgcgacgggc
     1981 gcgccgcccg cgcctggatc cagaggcagc tcaaggcgga ggggcagggg cagccggccc
     2041 tcgtcgtgct tctgctggcg gtgagccgct tcgacacgat caacgccgcc ttcggccgcc
     2101 cgaccgggga cgccgtcctc caggccgccg cccggaggat cgagcggctc gccgattcgg
     2161 acgggcgccg ccgcctcgtc gcccgaatcg ccggggccga gttcgcaatc ctcctggccg
     2221 cgcccaccag cctcggcgag ggacggttcc tggccgggca actggtcgag gcgatcggtc
     2281 gcccgttcat gtctggagac catgtcatca ccctcggcag ccgggtcggg gtggtcgcct
     2341 ccgaagggga cgaggacgcc gcgagccttc tgcggcgggc gagcgcggct ttggccgagg
     2401 ccaagtcgag cgaggcgggg ccggtcaagg tcgtcgacgc cggcgcggcc agcgacagcg
     2461 ctctcggcga ccagctcgag atcgacctca ggcgagcgct cgaccaggac gagatcgaga
     2521 tcctcttcca gccgcaggtc tcgatcgcca accgtcgaat catcggcgcc gaagcgctcg
     2581 cccgctggcg ccatccggag ttcggcgagc tc
//
LOCUS       DASWNU010000438         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_146258, whole genome shotgun sequence.
ACCESSION   DASWNU010000438 DASWNU010000000
VERSION     DASWNU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146258"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VGB57_08555"
     CDS             <1..1408
                     /locus_tag="VGB57_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0131444.1"
                     /translation="AVEGLFEQVRSNLDDAQAMAMYDPVTALPNRLHFRREAERILST
                     RDSGAALLFIDLDGFKEVNDRLGHAQGDQVLAMVANRLRVVLKAEVADGTLAPPLLAR
                     LAGDEFTMLLPEVSTRDEAERIAARALAALAEPFKSPVGSVLIGGSIGIALAPEHGSD
                     LAGLMKAADIAMYHAKASGRSRLCSYEPSLARASEERSALEASVRRAVERHELELVFE
                     PRLCLRTGAITAAEAQIRWNGAGGSRLLSGEESILQETGLAVRLGEWTLDAVSGALER
                     WRAAGLDHRLCFPVATRQFERLDFIERLRAALARAGAPPWGVEIELNEAAASGCDKWV
                     MAELERLRLEGVEVCVSDFGSGRARLSGFASLPMNRVRLDSGIVADIDRSERARSVAA
                     SLIHLVHGLGCEAVAAGAERQDQVEVLRALGCDSLQGFPGVAPMPEDAFLAWAEAQDC
                     ARSLAHDVYASAPLTMRA"
     gene            complement(1375..2493)
                     /gene="dapE"
                     /locus_tag="VGB57_08560"
     CDS             complement(1375..2493)
                     /gene="dapE"
                     /locus_tag="VGB57_08560"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY0131445.1"
                     /translation="MTLAIDPVELASELIACPSVTPAGGQAFDTLEAALRPLGFEVHR
                     FVSGGEVENLFATRGTGSPHFGFAGHLDVVPPGEGWSGDPFRPEIRGGLLYGRGAVDM
                     KGGIAAFVAAAAQVPEHEGTLSLLITGDEEGPAIHGTRAIMAWMAERGIRPDMILIGE
                     PTSEARLGDTVKIGRRGSVNMWINVPGVQGHVAYPHRADNPVPKLARAIAALESLHLD
                     DGSDSFQPSNLEVTAVDTDTKATNLIPGSARIQLNIRFNNLHRGEDLVALVRRTAEAH
                     APGASVEAMISGEAFLTPAGPLYELVTRAILEETGVEPVLSTSGGTSDGRFLIELCPV
                     VDFGLPNATMHKLDEAASAEDIRALARIYARIVRGALA"
     gene            2545..3036
                     /locus_tag="VGB57_08565"
     CDS             2545..3036
                     /locus_tag="VGB57_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0131446.1"
                     /translation="MPKLDLDAFPQINTTGYPSPWREEMAKRLYRRLGPASGLSDFGV
                     SHVTLEPGGISSQRHWHEGEDEFVVMLEGEAVLEEEGGETAIRAGDCAAFPKGVANGH
                     RLVNRGNSPCVFIAVGKWATTDCHYPDIDLHLDGATSRFTRKDGTAYCPPSSRRKPGP
                     QGV"
BASE COUNT          444 a         1091 c         1040 g          473 t
ORIGIN      
        1 cgccgtcgag ggattgttcg agcaggtccg ctccaacctg gacgacgccc aggcgatggc
       61 gatgtacgat ccggtgaccg cgcttcccaa ccgcctccac ttccgccgcg aggcggagcg
      121 gatcctgtcc acccgcgaca gcggcgcagc cttgctgttc atcgacctcg acggcttcaa
      181 ggaggtcaac gaccgcctcg gccacgccca gggcgaccag gtcctcgcaa tggtcgccaa
      241 ccgccttcgg gtcgtgctca aggccgaagt cgccgacggc accctggcgc cgcctctgct
      301 cgcccggctc gccggcgacg agttcaccat gctccttccc gaagtctcca cccgcgacga
      361 ggctgagcgg atcgcggcgc gggcgctggc agcgctcgcc gagccgttca aatcgccggt
      421 cggatccgtc ctcatcggcg gctcgatcgg catcgcgctg gcgcccgagc acggctccga
      481 tctcgccggg ctgatgaagg cggcggacat cgccatgtac cacgccaagg cctccggccg
      541 ctcgcgcctg tgcagctacg agccgagcct cgcccgggcc tcggaggaac gcagcgcgct
      601 cgaggcttcg gtccgccgcg ccgtcgagcg gcatgagctg gagctcgtct tcgagccccg
      661 cctgtgcctt cgtaccggag ccatcaccgc cgccgaagcg cagatacgct ggaacggcgc
      721 gggcggaagc cggctcctct ccggcgagga gtcgatactc caggaaaccg gtctcgccgt
      781 tcggctcggc gaatggactc tcgacgccgt gtcgggggcg ctcgaacgct ggcgcgccgc
      841 cggcctcgac caccgcctct gcttcccggt cgccacccgc cagttcgagc ggctcgattt
      901 catcgagcgt ctgcgcgccg ccttggcccg ggcgggtgcc ccgccctggg gagtcgagat
      961 cgagctcaac gaagccgcgg cttcaggatg cgacaaatgg gtgatggcgg agctcgagcg
     1021 gctgcgcctg gagggagtcg aggtctgcgt cagcgacttc gggtccggcc gggcgcgcct
     1081 ctccggcttc gccagcctgc cgatgaaccg ggttcgcctg gacagcggga tcgtcgccga
     1141 catcgaccgc tcggagcggg cgaggagcgt cgccgcctcg ctcatccacc tcgtccacgg
     1201 ccttggttgc gaggcggtgg ccgccggcgc ggagcggcag gaccaggtcg aggtgcttcg
     1261 cgccttgggc tgcgacagcc tgcagggctt cccgggcgtc gctccgatgc ccgaggacgc
     1321 cttcctggcc tgggccgagg cgcaggattg cgcccgaagc ctcgcccatg acgtctacgc
     1381 cagcgcgccc ctgacgatgc gggcgtagat ccgggcgagg gctcggatgt cctcggccga
     1441 agccgcctcg tccagcttgt gcatggtggc gttgggaaga ccgaagtcga cgaccgggca
     1501 gagctcgatc aggaagcggc cgtcggacgt gccgccgctg gtcgacagga ccggttccac
     1561 ccccgtctcc tctagaatcg cgcgagtcac gagctcgtag agcggccctg ctggagtgag
     1621 aaaggcctcg cccgagatca tcgcctcgac gctcgctccg ggtgcatgcg cttccgccgt
     1681 ccgccgaacc agagcgacga gatcctcgcc ccggtgaagg ttgttgaagc ggatgttgag
     1741 ctggatccgg gcgcttccgg ggatgaggtt ggtcgccttg gtgtccgtgt cgaccgcggt
     1801 cacctcgagg ttggaaggct ggaacgagtc gctcccgtcg tcgaggtgga gcgactccag
     1861 cgcggcgatc gctctcgcca gcttcgggac cggattgtcg gcccggtggg gataagcgac
     1921 gtgcccctga acgcccggga cattgatcca catgttgacg gagccgcggc ggccgatctt
     1981 gaccgtgtct ccgagccggg cctccgaggt cggctctccg atcaggatca tgtcgggccg
     2041 gatgccacgc tccgccatcc acgccatgat ggcgcgcgtg ccgtggatcg ccgggccttc
     2101 ctcgtcgccg gtgatgagca ggctgagcgt gccttcatgc tcgggcactt gcgccgcggc
     2161 ggcgacgaag gcggcgattc cgcccttcat gtcgaccgct ccgcgcccgt agagcagccc
     2221 cccccgtatc tccgggcgga acggatcccc gctccagcct tcgcccgggg gcacgacgtc
     2281 gaggtggccg gcgaagccga aatgggggct gcccgtgccg cgcgtggcga acagattctc
     2341 gacctctccg ccgctgacga agcggtggac ctcgaaaccg agcggcctca gcgccgcttc
     2401 cagcgtgtcg aacgcttggc cgccggcggg cgtgacgctt gggcaggcga tcagctccga
     2461 cgcgagctca accgggtcga ttgcaagggt catgcggctt cgcctagcaa gggggcgtcc
     2521 gcttgcccag ccaaaggccc ccgcatgccc aagctcgacc tcgacgcctt cccgcagatc
     2581 aacacgaccg gctatccctc gccctggcgc gaggagatgg cgaagcgcct ttaccgccgt
     2641 ctcggcccgg cctcgggcct gtccgacttc ggggtcagcc acgtcaccct cgagcccggc
     2701 ggcatctcca gccagcgcca ctggcacgaa ggcgaggacg agttcgtcgt catgctcgaa
     2761 ggcgaggcgg tgcttgagga ggaagggggc gagacagcga ttcgggcagg cgactgcgcc
     2821 gcctttccca aaggggtcgc caacggccat cgcctggtca atcgcggcaa ctcgccgtgc
     2881 gtcttcatcg ccgtcggcaa gtgggccacg accgattgcc attaccccga catcgatctc
     2941 cacctggacg gcgccaccag ccgcttcacc cgcaaggacg gtacggctta ttgtcccccg
     3001 tcatcccggc gcaagccggg accccagggg gtgtagaacg ccggcgcc
//
LOCUS       DASWNU010000439         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_146316, whole genome shotgun sequence.
ACCESSION   DASWNU010000439 DASWNU010000000
VERSION     DASWNU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146316"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /gene="thiC"
                     /locus_tag="VGB57_08570"
     CDS             complement(<1..1473)
                     /gene="thiC"
                     /locus_tag="VGB57_08570"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY0131447.1"
                     /translation="MAETGTIPTGERAGSRKVYVAGELFPDIRVPFREVAVHPSANEP
                     PVTIYDSSGPYTDPTAVIDIKRGLPLVKSSWQLDRGDIAPVLNPREVKPEDNGHAAGK
                     HLAPRFDTSRHKVFKGVPGRPVTQYEYANAGIITPEMEYVAIRENLRREQTAPCIRDG
                     EDFGASIPDFVTPEFVRQEIARGRAIIPHNINHPEVEPMIIGRNFLVKINANIGNSAV
                     LSSVDDEVDKLVWATRWGADNVMDLSTGRNIHNIRDWIIRNSSVPIGTVPIYQALEKV
                     NGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVRLPFVPLTARRVTGIVSRGGSIMAK
                     WCLSHHRESFLYEHFEDICDIMRAYDVSFSLGDGLRPGSIADANDEAQFAELRTLGEL
                     TKIAWAKGCQVMIEGPGHVPMHKIKANMDKQLATCGEAPFYTLGPLTTDIAPGYDHIT
                     SAIGAAMIGWFGTAMLCYVTPKEHLGLPDRQDVKDGVITYK"
     regulatory      complement(1605..1713)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1766..2521)
                     /locus_tag="VGB57_08575"
     CDS             complement(1766..2521)
                     /locus_tag="VGB57_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HEY0131448.1"
                     /translation="MVGLAVLFLLMQGGPDIGVEALGPSTYRLTITVENETDPVSVSR
                     LLIPKARALCGDQGYELGHYSFTATQEVPSLEAPRGGPGSLTLVQDMTCGRTAEVAPA
                     ALATPITDAEATALQPGVLALTEQYFAALDTGAFAEAFAMADEAMTGGQSLEDWTADA
                     RAQQTSVGALTARQVGRVTWYSNPPGTEPGIYVAVDFVADGAVQDLCGYLVWRRSIDG
                     SGFRLTRQEIVEIPHDLSTEALAAVRRQYCILL"
BASE COUNT          400 a          836 c          968 g          463 t
ORIGIN      
        1 cttgtaggtg atgacgccgt ccttgacgtc ctgacggtcg ggcaggccga ggtgctcctt
       61 gggcgtgacg tagcagagca tggccgtgcc gaaccagccg atcatggccg cgccgatggc
      121 gctggtgatg tggtcgtagc cgggcgcgat gtcggtggtc agcggcccca aggtatagaa
      181 cggcgcctcc ccacacgtcg ccagctgctt gtccatgttg gccttgatct tgtgcatcgg
      241 cacgtggccg gggccctcga tcatcacctg gcagcccttg gcccaggcga tcttggtgag
      301 ttcccccaac gtgcgcagct cggcgaactg ggcctcgtca ttggcgtcgg cgatggagcc
      361 ggggcgcagg ccgtcgccga ggctgaacga cacgtcgtag gcccgcatga tgtcgcagat
      421 gtcctcgaag tgctcgtaga ggaaggactc gcggtggtgg ctgaggcacc acttggccat
      481 gatggagccg cctcggctga cgatgccggt gacccttctt gccgtcagcg ggacgaacgg
      541 gagacgcacg ccggcgtgga tggtgaagta gtcgacgccc tgctcggcct gttcgatcag
      601 ggtgtcgcgg tagacctccc aggtcaggtc ctcggcgacg ccgttcacct tctccagcgc
      661 ctgatagatc ggcaccgtcc cgatggggac gctggagttc ctgatgatcc agtcgcggat
      721 gttgtggatg ttgcggcccg tcgacaggtc catgacgttg tcggcgcccc agcgggtggc
      781 ccagaccagc ttgtcgacct cgtcgtcgac ggagctgagg accgccgagt tgccgatgtt
      841 ggcgttgatc ttcaccagga agttgcggcc gatgatcatc ggctcgacct cggggtggtt
      901 gatgttgtgc gggatgatgg cgcggccgcg ggcgatctcc tgacgcacga actcgggggt
      961 gacgaagtcc ggaatgctgg cgccgaagtc ctccccatca cgaatgcagg gcgcggtctg
     1021 ttcgcggcgc agattctcgc ggatggcgac gtattccatc tcgggggtga tgatcccggc
     1081 gttcgcgtac tcgtactggg tgaccgggcg gccggggacg cccttgaaca ccttgtggcg
     1141 gctggtgtcg aagcgggggg ccaggtgctt gccggcggcg tggccgttgt cctcgggctt
     1201 gacctcgcgg gggttcagga cgggggcgat gtcgccgcgg tcgagctgcc acgacgactt
     1261 caccagcggc aggccgcgct tgatgtcgat gacggcggtc gggtcggtgt aggggccgga
     1321 gctgtcgtag atcgtcaccg gcggctcgtt ggccgagggg tggacggcga cctcgcggaa
     1381 ggggacgcgg atgtcgggga agagctcgcc ggcgacatag accttgcggc tgccggcgcg
     1441 ctcgccggtg gggatggtcc cggtctcggc catcacctga tcacgcgcgt ccttcagcgc
     1501 cagatcgacg gccggctcgg cgggcagggt gacggcgatg ttcatggcgg cggcctcagg
     1561 ttgagggcca cgcgcggcgg aaagccggtt ttcgggcgga gatggagcgc cgcccgttcc
     1621 catcccttcg ccggcatgac ccggatcagg ttcgacgggt caggcgatcg cgccaatctc
     1681 agcggcctag gcgccgcccc ccggagaacg gccggagact acgcctcacg agaaggacgt
     1741 gtcgagcgtg gcaggcgcgc gctggtcaaa gaaggatgca gtactggcgc cggaccgccg
     1801 cgagcgcctc ggtgctcaga tcatgtggga tctcgacaat ttcctgccgt gtgagacgga
     1861 atccggagcc gtcgatgctg cgccgccaga cgaggtagcc gcacaaatcc tgcacggcgc
     1921 catccgccac gaaatctacc gcgacgtaga tcccaggttc ggtcccgggc gggttggaat
     1981 accaggtcac ccggccaacc tgccgggcgg tgagggcccc gactgaggtt tgctgcgccc
     2041 gagcgtccgc ggtccagtct tcgagcgact gccctccggt catggcttca tccgccatcg
     2101 cgaaggcctc ggcgaaggct ccggtgtcca gggccgcgaa atattgctcg gttaacgcca
     2161 ggaccccggg ctgcagcgcc gtcgcctcgg cgtcggtgat cggcgtcgcg agcgccgcgg
     2221 gcgcgacctc ggcggtccgg ccgcaggtca tgtcctggac gagtgtgagc gatcccggtc
     2281 cgcccctcgg ggcctcgagg ctcgggacct cctgggtcgc cgtgaagctg taatgcccga
     2341 gttcgtagcc ctggtcgccg cacagcgccc gcgccttggg gatcagcagg cgggatacgg
     2401 agacgggatc ggtttcgttc tcgacggtga tcgtcaggcg ataggtcgac ggacccagcg
     2461 cctcgacgcc aatgtccggc ccgccctgca tgaggagaaa tagaaccgcc agtccgacca
     2521 tcgaaacatc cccttcgatc gagcaggagt gagtcggcag ggagcgtgag ccgagtgcgc
     2581 cccaaaagct accgcttcag cgtctccgcg tacgcgaggc ccgccgcgtc atgctgtcgg
     2641 ccggcggcct ggatggcctg gaagcgg
//
LOCUS       DASWNU010000440         6838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_485659, whole genome shotgun sequence.
ACCESSION   DASWNU010000440 DASWNU010000000
VERSION     DASWNU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485659"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGB57_08580"
     CDS             complement(<1..221)
                     /locus_tag="VGB57_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131449.1"
                     /translation="MRIQESLVGAAAAVALLSTTACTTNAEGERRVSTKGAIGGVVGA
                     VAGYFGGDLIGGRRDRTEKILGAGIGAVAG"
     gene            348..1679
                     /locus_tag="VGB57_08585"
     CDS             348..1679
                     /locus_tag="VGB57_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0131450.1"
                     /translation="MPPAPPLPPFPWFDVVIILMLIALNGVFAMAEMAIVSSRKPRLH
                     AMAKSGRWGAQTALDLASDPGRFLSTVQIGITLIGILAGAYSGASLGGPVGARLAPLG
                     LAPETAATLGFVLVIFVTTYASLVIGELVPKQFALRSPEPIAAIIAPPMLWTARATAP
                     LVWLLDRTSALIFHLLRMRRDNSDKVTAEELHLVVAEAHHAGVLEEYERAIISGVVRL
                     ADRPAREVMTPRTDVDWIDAQADAEEIRRTLVGIPHSRIPVADGSVDNLIGIVQARDL
                     VAALIEGRPLDLRALSREAPVVPDQMDAMDALAVLRGSETRMAFVHDEYGHFEGIVTS
                     ADLLNAISGVLAGGFEGGPEAPLVEREDGSWWVSGSLSADALSDRLGIGLPEDRDYAT
                     TAGFALSVLKHIPEVGERFAFGGWQFEIVDMDGFKIDKLLASEVVRKRRGG"
     gene            complement(1813..2298)
                     /locus_tag="VGB57_08590"
     CDS             complement(1813..2298)
                     /locus_tag="VGB57_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131451.1"
                     /translation="MWTVAQDNLILIVVVLLIGLAVALWAFRKSGSRADLTSDRRTDG
                     PEGNGLQDEFAAATQDVAGEILGVDAHPDIPGPSGPPDNLRLLKGVGPKMVAQLNEFG
                     ITRFDQLAGLTETEIALLDKRMGAFEGRIARDRLVEQACYLERGDKDGFEAKFGKLGG
                     A"
     gene            complement(2322..2756)
                     /locus_tag="VGB57_08595"
     CDS             complement(2322..2756)
                     /locus_tag="VGB57_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131452.1"
                     /translation="MPPVLKATLGLLLALAAAWIWHAPLGHGERLIERLEAEARREVA
                     KAELPGMKVNLSRSPLARTAILSGPANDLQREGLGSQKGVADYARDVEGISEVRWADE
                     PGAGRRVMPLLVETLAAAALAYLIGLGLGWLLRGRRRHEDFD"
     gene            complement(2793..4403)
                     /locus_tag="VGB57_08600"
     CDS             complement(2793..4403)
                     /locus_tag="VGB57_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0131453.1"
                     /translation="MRHFLVLTLATALAACASHAPPEPEPLPSPPEPVMMSPPPPPPV
                     PMPMEPMQSFAGPSSLAAMSPEQRERYQALQANPVKRVAEEPVSTFSVDVDTGSYANV
                     RRFLTQGQMPPADSVRVEEMLNYFRYDYPLPSDRSRPFSVRTDVSATPWNPDSRLLRI
                     GLRGYDLDRRERPAANLVFLVDTSGSMNEPDKLPLVKAALSLLADQLQPQDRVSIVAY
                     AGSAGLVLEPTADPAKVKAALSQFEAGGSTAGGQGLELAYATARATRIADGINRIILA
                     TDGDFNVGISDTRQILDMVRRNRDDGITLTTLGFGQGNYNEAMMEQVADAGNGNYAYI
                     DSAMEARKVLDQELSSTLFTIARDVKVQVEFNPAIVSEYRLIGYENRLLREEDFNNDA
                     VDAGDIGAGHMVTALYEIVPVGSRGWLGERRYEANRPQGPARPGGELAFVQLRYKLPN
                     EENSRLIQQPIPASAIAAARPPTGDMAFAAAVAAYGQKLKGDERLGRFGWEQIGALAG
                     PAETYWRQEFLQLIGLAAARQPAPAATQ"
     gene            complement(4507..5535)
                     /locus_tag="VGB57_08605"
     CDS             complement(4507..5535)
                     /locus_tag="VGB57_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131454.1"
                     /translation="MAAAPDFAAITTDAAWLPNRYDESQDLVHFVRLTREEHRAVTFI
                     TDEYIPAEAPRLVLRRSDVAAAAGPPAPMHFIFHSAYCCSTVLARAVDIEGVSMGLKE
                     PLILIDLVGWRRRGADPRQLAQRLDEVLTLLARPFAPGEAVVVKPSNVLNGVAPAMLG
                     LRPRSRAILLYAPLPDFLRSIARKGLWGRRWVRELFIGQLRDGMVDLGLGEQHYLDLT
                     DLQVAAAGWLAQHGAFAGMVERFGAARVRTLDSATLLARPGDAMTALSDLFGLGLDPP
                     AVERILAGPAFTSHSKSNAGFSAADRLAEQRDAAETHADEIEKVAVWAEAVAAAAGLS
                     MQLKAPLL"
     gene            complement(5535..6818)
                     /gene="purD"
                     /locus_tag="VGB57_08610"
     CDS             complement(5535..6818)
                     /gene="purD"
                     /locus_tag="VGB57_08610"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0131455.1"
                     /translation="MNVLLLGSGGREHALAWRLAQSPLLGKLFAAPGNPGIAEFAQIV
                     AIDPADHRATVDFCLKNSVELIVVGPEAPLVDGLADNLRTMGFCVFGPNRVPAQLEGS
                     KGFTKDLCARHGIPTARYVRASDRLAAEAALEDFTAPLVVKADGLAAGKGVVICATRE
                     EAQAALDTMFDGSFGAAGSAIVIEEFLEGEEASFFALTDGIAIVPFGSAQDHKRVGDG
                     DTGPNTGGMGAYSPAAVLTPELEARAMDEIVRPTVEALAARGTPYSGILYAGLMLTDE
                     GPKLIEYNVRFGDPECQVLMMRLESDLLELMYAVARGRLADSEPPRFSAGAALTVVMA
                     ANGYPGPPETGGAIEGLDRAGGEDVRVFHAGTALDGDRLVARGGRVLNVTAAGGSVAE
                     ARDRAYEAVGRIGFPSGFCRSDIGWREIARSGGTG"
BASE COUNT         1040 a         2355 c         2367 g         1076 t
ORIGIN      
        1 ccggcgacgg cgccgatgcc cgcgccgagt atcttctcgg tgcggtcgcg ccgtccgccg
       61 atcaggtcgc cgccgaaata gccggcgacg gcgccgacga ctccgccgat cgcgcccttg
      121 gtcgagaccc ggcgctcgcc ctcggcgttg gtggtgcagg ccgtggtgga gagaagggcg
      181 acggccgcgg ccgcgcccac aaggctctcc tggatacgca tacagattcc tttcccgcga
      241 gggactaatc gaacgagcga tcaaccgcct gcccgagcgg cttgttccat gtggggtacg
      301 aagcgcttgt cgcggagaaa gcttcccggc taagagccat gggcgccatg ccgcccgccc
      361 cccccttgcc accattccct tggttcgacg tcgtcatcat cctgatgctg atcgcgctca
      421 acggcgtgtt cgcgatggcg gagatggcga tcgtctcgtc gcgcaagccc cggctccatg
      481 cgatggccaa gtccgggcga tggggcgccc agacggcgct cgacctcgcg tccgatcccg
      541 gccgcttcct ttccacggtc cagatcggga tcaccctgat cgggatcctc gccggcgctt
      601 attcgggcgc cagcctcggc gggccggtag gcgctcgcct ggcgccgctc ggcctcgctc
      661 cggaaaccgc ggcgaccctc ggcttcgtcc tcgtcatctt cgtcaccacc tatgcatcgc
      721 tggtgatcgg cgagctggtg cccaagcagt tcgcgctgcg ttcgcccgag ccgatcgccg
      781 ccatcattgc gccgcccatg ctctggaccg ccagggcgac ggcgccgctc gtctggctgc
      841 tcgaccgcac cagcgccctc atcttccacc tcctgaggat gcggcgcgac aattccgaca
      901 aggtgaccgc ggaggagctt catctggtgg tcgcggaggc gcaccacgcg ggcgtgctcg
      961 aggaatatga gcgcgccatc atctcgggcg tcgttcgcct cgccgaccgg ccggcgcggg
     1021 aggtgatgac ccctcgcacc gacgtcgact ggatcgacgc ccaggctgac gccgaggaga
     1081 tccgccggac cctggtcgga atcccgcaca gccggatccc ggtcgcggac ggctcggtcg
     1141 acaacctcat cgggatcgtc caggcgcgcg acctcgtcgc cgccctcatc gaaggtcgcc
     1201 cgctcgacct gcgcgcgctc agccgcgagg ctccggtcgt tccggaccag atggatgcga
     1261 tggacgcgct cgcggtcctt cgcggaagcg agacaaggat ggccttcgtc catgacgaat
     1321 acggccattt cgaaggcatc gtgacctccg ccgatctgct caacgccatt tccggcgtgc
     1381 tcgccggcgg cttcgagggc gggccggaag cgcccctggt cgagcgcgag gacggcagct
     1441 ggtgggtctc cggctcgctc tccgccgacg ctttgtccga ccggctcggg atcggcctgc
     1501 ccgaagatcg cgactatgcg acgacggcgg gcttcgcttt gtccgtcctg aaacacatcc
     1561 cggaggtggg cgagcgcttc gccttcggcg gctggcagtt cgagatcgtc gacatggacg
     1621 gcttcaagat cgacaaattg ctggccagcg aagtggtgag gaagaggcgc gggggatgaa
     1681 atccttaatc ctcccccgga tttccggggg agggggacca tgcgaagcat ggtggagggg
     1741 ccttgccgac tccggagaac ccctccaccg cgcttcgcgc ggtccccctc cccgagaaat
     1801 ctcggggagg aattacgcgc cgcccaattt cccgaacttc gcctcgaagc cgtccttgtc
     1861 gccgcgctcc agatagcagg cctgctcgac cagccggtcg cgggctatcc tcccttcgaa
     1921 ggcgcccatg cgcttgtcga gcaaggcgat ctcggtctcg gtcaggccgg ccagctggtc
     1981 gaagcgggtg atcccgaatt cgttcaattg cgcgaccatc ttgggcccga cgcccttcag
     2041 gaggcggagg ttgtccggcg gtccgctcgg gccgggaatg tcgggatggg cgtcgacccc
     2101 caatatctcg ccggcgacgt cctgggtggc ggcggcgaac tcgtcctgaa ggccgttgcc
     2161 ctccggcccg tcggtgcggc ggtccgacgt gaggtccgcc cggctcccgc tcttgcggaa
     2221 cgcccagagc gcgaccgcga gcccgatcag caggacgacg acgatcagga tgagattgtc
     2281 ttgggcgacg gtccacatat tcggtcaggt ccttcttcgt gtcagtcgaa atcctcgtgc
     2341 cggcggcggc cccggaggag ccagccgagg ccgaggccga tgagataggc gagcgcggcg
     2401 gcggccaggg tctcgacgag cagcggcatg accctccgcc cggcgccggg ctcgtccgcc
     2461 cagcgcacct ccgaaatacc ctcgacgtcg cgggcatagt cggcgacgcc cttctggctg
     2521 ccgaggccct cgcgctggag atcgttggcg ggaccgctca ggatcgcggt gcgggcgagc
     2581 gggctccggg agaggttcac tttcatgccg ggcagctccg ctttcgcgac ctcccgcctg
     2641 gcctcggcct cgagccgttc gatcagccgt tcgccgtggc cgagcggcgc gtgccagatc
     2701 cacgccgcgg ccagcgccag gaggagaccg agggtcgctt tgaggacggg cggcatgacc
     2761 ctcccttttc ccgaaatcgc gcgccgcgca agctattgag tcgccgccgg cgccggctgc
     2821 cgggcggcgg cgaggccgat cagctgcagg aattcctggc gccagtaagt ctccgccggc
     2881 ccggcgagcg cgcctatctg ctcccagccg aagcggccga gccgctcgtc ccccttgagc
     2941 ttctggccgt aggcggcgac cgccgccgcg aacgccatgt cgcccgtcgg aggccgggcg
     3001 gcggcgatcg ccgacgcggg tatcggctgc tggatcaggc gcgaattctc ctcgttcggc
     3061 agtttgtagc ggagctggac gaaggcgagc tcgccgccgg gacgggcggg tccttgcggc
     3121 cggttggcct cgtagcggcg ctcgccgagc cagccccggg atccgacggg cacgatctcg
     3181 tacaaggcgg tgaccatgtg cccggcgccg atatcgcccg catcgacggc gtcgttgttg
     3241 aaatcctcct cgcgcagaag ccggttctca tagccgatca acctgtattc ggagacgatc
     3301 gccgggttga actcgacctg gaccttgacg tccctggcga tggtgaagag ggtcgagctc
     3361 agctcctggt cgagcacctt ccgggcttcc atggcactgt cgatataggc gtaattgccg
     3421 ttcccggcgt cggcgacctg ctccatcatc gcttcgttgt aattgccctg tccgaagccg
     3481 agcgtcgtca gggtgattcc gtcgtcgcgg ttgcggcgga ccatgtccaa tatctgccgg
     3541 gtgtccgaga ttccgacgtt gaagtcgccg tcggtggcga ggatgatccg gttgatgccg
     3601 tcggcgatcc gggtggcccg ggcggtggca taggcgagct ccaggccctg gccgccggcg
     3661 gtggagccgc cggcctcgaa ctgagacaaa gccgccttga ccttggcggg atcggcagtg
     3721 ggctcgagaa caaggccggc cgagccggca taagcgacga tcgagacccg gtcctgcggc
     3781 tgtaactggt cggcgagcag cgacagggcc gccttgacca gaggcagctt gtccggctcg
     3841 ttcatcgagc cggaggtgtc gaccaggaag acgaggttcg ccgccggccg ttcccgccgg
     3901 tccagatcgt agccgcgcag gccgatccga agcaggcggc tgtcggggtt ccacggcgtg
     3961 gccgagacat cggttcggac cgagaagggc cggcttcggt cgctcggcag cggataatcg
     4021 tagcggaagt aattgagcat ctcctcgacc cggaccgagt cggccggcgg catctgcccc
     4081 tgggtcagga atcggcggac gttggcgtag ctgcccgtgt cgacgtcgac ggaaaaggtg
     4141 ctgaccggct cctcggcgac ccgcttgacc gggttggcct gaagcgcctg atagcgttcc
     4201 ctctgctccg ggctcatcgc ggcgaggctc gaaggtccgg cgaagctctg cattggctcc
     4261 atcggcatcg ggaccggcgg aggcggtggc ggcgacatca ttaccggctc gggcggcgaa
     4321 ggcagcggtt cgggctccgg cggagcgtgg gaagcgcagg cggccagtgc cgtggccagg
     4381 gtcaaaacca ggaaatgtct catccgcccc ctccccgata ggagaggcca gcctagcaac
     4441 tcgccggcgc agggcaagcg tcggcgctga cttgcccctc tcccgcgaaa aaccgggaaa
     4501 ggggtgttag agcagcggcg ccttcagctg catcgacagc ccggcggccg cagcgaccgc
     4561 ttccgcccag accgcgactt tctcgatctc gtccgcgtgc gtctccgcgg cgtcgcgctg
     4621 ctcggcgagg cggtcggcgg cgctgaagcc ggcgttggac ttggaatggc tggtgaaggc
     4681 cggcccggcg aggatccgtt cgaccgccgg cgggtcgagg ccgaggccga acaggtcgga
     4741 cagcgcggtc atcgcgtcgc ccggacgagc caacaaggtg gcgctgtcga gggtccggac
     4801 ccgagccgcc ccgaaccgct cgaccatccc ggcgaaggcg ccatgctgcg ccagccagcc
     4861 cgccgccgcg acctggagat cggtgagatc gagataatgc tgctcgccta ggccgagatc
     4921 gaccattccg tcgcgcagct ggccgatgaa cagctcgcgc acccagcgcc gtccccacag
     4981 ccccttgcgc gcgatcgagc gcaggaaatc gggcagcggc gcatagagca ggatcgccct
     5041 agatcgcggc ctcagcccga gcatggccgg ggcgacgccg ttcaggacgt tggagggttt
     5101 cacaacgacg gcctcgcccg gcgcgaatgg ccgggcgagc aaggtcagta cctcgtcgag
     5161 tctctgggca agctggcgag ggtcggcgcc gcggcgcctc cagccgacca gatcgatgag
     5221 gatgaggggt tccttgaggc ccatcgagac gccttcgatg tcgacggcgc gggccaggac
     5281 ggtcgagcag caataagccg aatggaagat gaaatgcatc ggcgcgggcg gccctgccgc
     5341 ggcggcgacg tcgcttcgcc tgagcacgag cctcggcgct tcggcgggga tatattcgtc
     5401 ggtgatgaag gtgacggcgc gatgctcctc gcgggtcagc cggacgaagt ggaccagatc
     5461 ctggctttcg tcgtagcggt tgggaagcca ggccgcgtcg gtggtgatcg cggcgaagtc
     5521 gggcgccgcc gccatcagcc ggtcccgccg gagcgggcga tctcccgcca gccgatgtcg
     5581 ctgcggcaga aaccggacgg aaagccgatc cgccccaccg cctcgtaagc ccggtcgcgc
     5641 gcctcggcga ccgagccgcc ggccgccgtg acgttgagga cccggccgcc gcgcgccacc
     5701 agccggtccc cgtccagcgc agtgccggcg tggaagaccc tgacatcttc cccgccggcc
     5761 cggtcgagtc cctcgatcgc tccccccgtc tcgggcgggc cgggatagcc gttcgccgcc
     5821 atcaccacgg tcagggccgc cccggccgag aagcggggcg gctcggagtc cgcgagccgg
     5881 ccgcgggcga ccgcgtacat cagctccaga aggtcgcttt ccagccgcat catcagcacc
     5941 tggcattcgg ggtcgccgaa gcgaacgtta tattcgatca gcttggggcc ctcatccgtc
     6001 agcatcaggc ccgcatagag gattcccgaa taaggcgtgc cccgggcggc gagggcctcg
     6061 acggtggggc ggacgatctc gtccatcgcc cgggcctcca gctcgggagt cagaaccgcc
     6121 gccgggctgt aggcgcccat tccgccggtg ttcgggccgg tatcgccgtc gccgacgcgc
     6181 ttatggtcct gggcggagcc gaaggggacg atcgcaatgc cgtcggtgag cgcgaagaag
     6241 ctcgcttcct ccccttcgag gaactcctcg atgacgatcg cgctcccggc cgcgccgaag
     6301 ctgccgtcga acatcgtgtc gagcgccgcc tgcgcttcct cccgggtcgc gcagatgacg
     6361 acgcccttgc cggcggcgag gccgtccgcc ttgacgacga gcggggcggt gaaatcctcg
     6421 agcgcggcct cggcggcgag cctgtcggac gcccggacgt agcgcgcggt cgggatgcca
     6481 tggcgggcgc aaagatcctt ggtgaagccc tttgacccct ccagctgcgc cggcacccgg
     6541 ttcgggccga agacgcaaaa gcccatcgtc cggagattgt ccgccagccc gtcgaccaga
     6601 ggcgcttccg ggccgacgac gatcagctcg accgaattct tcaggcagaa atcgaccgtg
     6661 gcgcggtggt ctgccgggtc gatcgccact atctgggcga actcggcgat gccgggattg
     6721 ccgggcgcgg caaacagctt gcccagaagc ggcgattgcg ccagccgcca ggcgagcgca
     6781 tgctcgcgac ccccggagcc aagcagcagg acattcatgg aaagcgacta ccctcctc
//
LOCUS       DASWNU010000441         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_406202, whole genome shotgun sequence.
ACCESSION   DASWNU010000441 DASWNU010000000
VERSION     DASWNU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406202"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1272
                     /locus_tag="VGB57_08615"
     CDS             205..1272
                     /locus_tag="VGB57_08615"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEY0131456.1"
                     /translation="MPVPRSGLLPALALTGAALALGGGASAQTPKIARPRPAPPGPST
                     MPALPPAAIDEALAIGGDDIDARKVETRLSVEVRVNGRGPYQFVVDSGADTSVVGLRI
                     ARDLQLPLGTPVTLNNMTDRNIVDRVRVESLSLGPSIIRNLQLPALREADLGGAGMIG
                     IDALARQRLMMDFEKRLIRVEDASRPAKSQPGDIVVTARLQRGQLILTEVKAGRVTLD
                     AVIDTGSQITIGNKALRDRLLRRRIGRFKTVTATGVTGKTVQLELALVGELRLGSVIL
                     RNVPMAFADVPPFEVFGLSDKPALLLGTDLLETFRRISLDFRARKVRFQLRRCRQGIA
                     ISTSPSASLTRLSASGQACAR"
     gene            complement(1291..>1522)
                     /locus_tag="VGB57_08620"
     CDS             complement(1291..>1522)
                     /locus_tag="VGB57_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131457.1"
                     /translation="PEDARTENRVVNRTAAVDALLEVDWIRGARPKTSRPAPPPGDRA
                     AAMAWWHDEVRGLNPLTDPNLPEAEVLRLLGD"
BASE COUNT          223 a          541 c          537 g          221 t
ORIGIN      
        1 gccgccggag cagcgcgaag agaccgcgcg gtccggccgg cgggtcgcgg agcgatcccg
       61 tccgacgtca cggggccgcc cccgtgacgg ccgcacccgg cggttggggt cacgcaaagc
      121 cgcgaaggcg caaagaggcc ctttgcatgg cgaatgtcgg gggtgcgttc gcctaacccg
      181 caagttccag gtgaaaggaa aagcttgccc gttccccgct ccggcctcct tcccgccctg
      241 gcgctgacag gcgcggcgct cgccctcggc ggcggagcct cggcgcagac gcccaagatc
      301 gccaggccca ggcccgctcc gccggggccc tcgaccatgc ccgcgctgcc gccggcggcc
      361 atcgacgagg cgctcgccat cggcggcgac gacatcgacg cgcgcaaggt cgagacccgg
      421 ctcagcgtcg aggtccgggt gaacgggcgg ggaccctatc agttcgtcgt cgacagcggc
      481 gccgacacgt cggtggtggg cctcaggatc gcgcgcgacc tgcagctgcc gctcgggact
      541 ccggtgaccc tgaacaacat gaccgaccgg aacatcgtcg accgggtgag ggtggagtcg
      601 ctgagcctcg ggccgagcat catcaggaac ctgcagctcc ccgcgcttcg cgaggccgac
      661 ctcggcggcg ccgggatgat cgggatcgac gcgctcgccc ggcagcggct gatgatggat
      721 ttcgaaaagc gcctgatcag ggtcgaggac gcctccaggc ccgccaagtc ccagcccggc
      781 gacatcgtcg tcacggcccg gcttcagcgc ggccagctga tcctgaccga ggtgaaggcc
      841 ggccgggtga cgctcgacgc ggtgatcgat accggctccc agatcacgat cggcaataaa
      901 gcgcttcgcg acaggctcct gcggcgaagg atcggcaggt tcaagacggt caccgcgacc
      961 ggggtgaccg gcaagacggt ccagctcgag ctcgctttgg tgggggagct ccggctcggc
     1021 tcggtgatcc ttcgcaacgt gccgatggcc ttcgccgacg ttccgccgtt cgaggtgttc
     1081 ggactttccg acaagccggc cctgcttctg ggcaccgacc tgctggagac cttccgccgc
     1141 atctcgctcg atttcagggc gcgcaaggtg cgcttccagc ttcggcggtg ccggcagggg
     1201 atcgcgatca gcacctcccc ttccgcgtcc ctgacccgcc tgtccgccag cggccaggcc
     1261 tgcgcgagat gaggcggacc ctcctgcccc tcagtcgccg agtagtcgaa ggacctccgc
     1321 ctcgggcagg ttggggtccg taagcgggtt caggccgcga acctcgtcat gccaccaggc
     1381 cattgcggcg gctcgatcgc ccggcggcgg tgcgggacgg gacgttttcg ggcgggcgcc
     1441 gcggatccag tcgacctcga gcagggcgtc gaccgcggcg gtccggttga cgacacggtt
     1501 ctcggtgcgc gcgtcctccg gc
//
LOCUS       DASWNU010000442         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_457614, whole genome shotgun sequence.
ACCESSION   DASWNU010000442 DASWNU010000000
VERSION     DASWNU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457614"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1012
                     /locus_tag="VGB57_08625"
     CDS             92..1012
                     /locus_tag="VGB57_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131458.1"
                     /translation="MTEPPASPPQAQPELMKWPALLERKRPEPDATIAYGEDQMQKVD
                     LWLPAGAGPHPTVMMVHGGCWQTEIADRRIMNWIADDLRKRGIAVWNIDYRGVDRSGG
                     GYPGTFVDAAAAADALRQHAPRYKLDISPLVAVGHSAGGHLALWLAARRAASRRPASP
                     LVPRGSPLIGADPLPIATVVSLGGLPDLELAATPPGSGCGTEVIEKLVGPPTLSSRSV
                     YADTSIPRLAPLGVRQVLINGLQDRIIPTAYAEDYASKMRAAGDKVKVRMIDATGHVE
                     LIAPESRAWAATVEEIEAALHRHSGGGRKP"
     gene            1080..2303
                     /gene="kynU"
                     /locus_tag="VGB57_08630"
     CDS             1080..2303
                     /gene="kynU"
                     /locus_tag="VGB57_08630"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEY0131459.1"
                     /translation="MTSLEQARALDEADPLRPFRDRFLLPEGLIYLDGNSLGALSKAA
                     VGRQRAMVEEEWGTELIRSWNTRGWIEAPQRVGAKIAPLIGAKPNEVIVADSTSVNLF
                     KLIVAAAALAPGRHQLLSEAGNFHTDLHIAAGAAELAGLRLDVVGRDSLDAAIGPDTN
                     LLLLTHVHFKAGFRFDMAGVTARAKAAGALTLWDLSHSAGAVPLDLNGDGAELAVGCG
                     YKYLNGGPGAPAFLYVAESLQDRLMPPLRGWMGHAEPFAFTDDYRPAPGIDRFLVGTP
                     PMLSLAALESGVESFEGADMAALWAKSIALFDLFADLVQAHCPELDCISPRDPERRGS
                     HISFRHPHAFELCQALIADGVIGDFRAPDVVRFGLTPLYLGYEDIWRAVERMRGILDS
                     GRWRDPRFAVRGKVT"
     gene            2409..4427
                     /locus_tag="VGB57_08635"
     CDS             2409..4427
                     /locus_tag="VGB57_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0131460.1"
                     /translation="MNAKSLLLAACAAMTVAALPAIAQTNVPLIPREKIFGNPTKVAG
                     RLSPDGKWLSWIAPRDGVLNIHVAPAANPAAVRALTSETKRPIRQYFWSPDSRQILFI
                     NDAGGDENFLLYGVDVTSGEQRTLTPFEKTRVRIINISNEVTDRILVGVNNRDPKWHD
                     VHSLDMRTGKLTPVLTNTGSYANFIADEQLNIRIAAKSRPDGGTDYYRVADNAVEAQP
                     FEQVTLEDAQTTQPVGFTADGKTLYWLDSRGRDTAAVVGQDVATGRKTVIAENARADI
                     GGAMTNPRTGRLEAYAVNYLKNEWVPLGGAVKSDLDFLKSRLKGEISVTSRTEADDKW
                     IVAVDPVTAPSSAHLYDRKAKTLTKLYTSRPELEGAPLAAMHPVEIKTRDGLNMVSYL
                     TLPPGADPDGDGKPSRAVPLVLVVHGGPWGRDSYGYDGTAQWLANRGYAVLSPNFRAS
                     TGFGKKFISAGDLQWGKKMQDDLNDAVDWAVGRGITTSDKVAIMGGSYGGYAVLAGLA
                     FTPDKFACGVDIVGPSNLATLLGTIPPYWEALRAQFYKRMGDPNTPEGKAYQREVSPL
                     YSADKIRRPLLIGQGANDPRVNVAESQQIVDALKAKNIPVTYVVFPDEGHGFARPQNN
                     IAFNAVAENFLARCLGGRAEPIGEALKPSSAQVPHGAEFAPGLAEALK"
BASE COUNT          768 a         1642 c         1455 g          711 t
ORIGIN      
        1 cgcgtttgcc agttagggcg gctgccgcta ccccgccccg atgcgccccg cccttgccct
       61 tgccctcctt acggcgctag ccgggtgctc ggtgaccgag cctcccgcct ctccgcccca
      121 agctcagccc gagctgatga aatggcccgc cttgctcgag cgcaagcgcc cggagccgga
      181 cgccacgatc gcctatggcg aagaccagat gcagaaggtc gacctctggc tccccgccgg
      241 cgccggcccg cacccgactg tgatgatggt tcacgggggc tgctggcaga ccgagatcgc
      301 cgaccggcgg atcatgaact ggatcgccga cgacctgcgc aagcgcggaa tcgccgtctg
      361 gaacatcgat tatcgcggag tcgaccggag cggcggcggc tatcccggca ccttcgtcga
      421 tgctgccgcg gcggcggacg cgctgaggca gcacgccccg cgctacaagc tcgacatctc
      481 gccgctcgtc gcggtcggcc attcggcggg cgggcatctc gccttgtggc tggcggcgcg
      541 gcgcgcggcg agccggcgtc cggcgagtcc gctcgtcccg cgcggcagcc cgctcatcgg
      601 cgccgatcca ctgcccatcg ccacggtggt cagcctcggc ggacttccgg acctcgagct
      661 cgccgccacg cctccgggca gcggctgcgg aaccgaggtg atcgagaaat tggtcggtcc
      721 gccgaccctg agcagccgaa gcgtctacgc cgacacgtcc attccccgtc tcgccccgct
      781 cggagtccgc caggtgctga tcaacggact ccaggaccgg atcatcccga ccgcttatgc
      841 ggaggattat gcgtcgaaga tgcgggccgc cggcgacaag gtgaaggtgc ggatgatcga
      901 cgctaccggc catgtcgagc tgatcgcgcc cgagtcccgg gcctgggccg cgacggtcga
      961 ggagatcgag gcggcattgc accgtcattc cggcggaggc cggaagccat gaacaccgcc
     1021 ggtggaatta cagacacgcc ggtgttcatg gaccccggcc ttcgcccggg tgacgagggg
     1081 tgaccagtct cgagcaagcc cgcgccctgg acgaggccga tccgcttcgc cccttccgcg
     1141 accgcttcct cctccccgaa ggcctcatct acctcgacgg caattcgctc ggcgctctct
     1201 ccaaagccgc ggtaggccgg cagcgggcga tggtcgagga ggaatggggc accgagctga
     1261 tccgaagctg gaacacgcgc ggctggatcg aggcgccgca gcgggtcggc gcgaagatcg
     1321 cccccctgat cggagccaag ccgaacgagg tcatcgtcgc cgattcgacc tcggtgaacc
     1381 tgttcaagct gatcgtcgcc gccgccgctt tggcacctgg ccgtcaccaa ctcctctccg
     1441 aggccggcaa tttccacacc gatctccata tcgccgccgg agcggccgag ctggccgggc
     1501 ttcgcctcga cgtcgtcggg cgcgactcgc tcgacgccgc gatcgggccg gacaccaacc
     1561 tgctcctgct cacccacgtc catttcaagg ccggcttccg cttcgacatg gcgggggtga
     1621 ccgcccgggc gaaggctgcg ggggcgctga ccttgtggga cctcagccac agcgccggcg
     1681 ccgtgccgct cgacctcaac ggcgacgggg ccgagctcgc cgtcggctgc ggctacaaat
     1741 atctcaacgg aggccccggc gctccggcct tcctctacgt cgccgaatcc ctgcaggacc
     1801 ggctgatgcc gcccctgcgc ggctggatgg gccatgccga gccgttcgcc ttcaccgacg
     1861 attatcggcc ggcgcccggc atcgatcgtt tcctggtcgg caccccgccg atgctcagcc
     1921 tcgccgcgct cgaaagcgga gtagagagct tcgagggcgc ggacatggcg gcgctctggg
     1981 ccaagtccat cgccctgttc gatctgttcg cggatctcgt ccaggcccac tgcccggagc
     2041 tcgactgcat ctcgccccgg gacccggagc gccgcggcag ccatatcagc ttccgccatc
     2101 cccacgcttt cgagctttgc caggccctga tcgccgacgg cgtgatcggc gacttccgag
     2161 caccggacgt ggtccgcttt ggcctcactc cgctttacct cggctatgag gatatttggc
     2221 gggcggtgga gcggatgcgg ggaatcctcg actccggccg ctggcgcgat ccgcgcttcg
     2281 ccgtgcgcgg gaaggtgacg taatttgcct ccctccccac ggcttcgacg cagggaggca
     2341 aaatgcactt gtgtgttagt atcataatac actatgatcg gacgaaatcc aaccgtgaga
     2401 ggtgcccgat gaatgccaag tccctgctgc ttgccgcctg tgctgccatg accgtcgcgg
     2461 cccttcccgc gatcgcccag acgaacgtgc cgctcatccc ccgcgagaag atcttcggca
     2521 acccgaccaa ggtcgcgggc cggctctctc ccgacggcaa atggctgtcg tggatcgcgc
     2581 cgcgcgacgg cgtgctcaac atccacgtcg ccccggccgc caatccggcc gcggtgcggg
     2641 cccttaccag cgaaacgaag cgccccattc gccaatattt ctggtctccg gacagccggc
     2701 agatattgtt catcaacgat gccggcggcg acgagaattt cctcctctac ggagtcgacg
     2761 tgacctccgg cgagcaacgg acgcttaccc ccttcgaaaa gacccgggtg cggatcatca
     2821 acatctccaa cgaggtgacc gaccgaatcc tcgtcggagt caacaaccgc gaccccaagt
     2881 ggcacgacgt ccacagcctc gacatgagga ccggcaagct cactccggtc ctgaccaaca
     2941 ccggcagcta cgccaacttc atcgccgacg agcagctcaa catccggatc gccgccaagt
     3001 cgcggcccga cggcggcacc gattattacc gggtcgccga caatgccgtc gaagcgcagc
     3061 ccttcgagca ggtgacgctg gaggatgccc agaccaccca gccggtcggc ttcaccgccg
     3121 acggcaagac gctctactgg ctcgacagcc gcgggcgcga cacggcagcc gtcgtcggcc
     3181 aggatgtcgc caccggccgc aagacggtga tcgccgaaaa cgcgagggcc gatatcggcg
     3241 gcgccatgac caatccgcgg accggacggc tcgaggccta tgcggtcaat tacctcaaga
     3301 acgagtgggt gccgctcggc ggggccgtca aatccgacct cgacttcctg aagagccgcc
     3361 tcaagggcga gatcagcgtc acttcccgca ccgaggccga cgacaagtgg atcgtcgcgg
     3421 tcgacccggt gaccgcgccc tcgagcgccc atctgtacga ccgcaaggcg aagacgctca
     3481 ccaagctcta caccagccgg ccggagctcg aaggcgcccc gctcgccgcg atgcacccgg
     3541 ttgagatcaa gacccgcgac ggcctcaaca tggtttccta tctcaccttg ccgccggggg
     3601 ccgacccgga cggcgacggc aagcccagcc gggcggtgcc gctggtgctg gtcgtccatg
     3661 gcggaccgtg gggccgggac agctacggct atgacgggac cgcccagtgg ctggccaatc
     3721 gcggctatgc cgtgctctcg cccaatttcc gggcctcgac cggcttcggc aagaagttca
     3781 tctcggccgg cgacctgcaa tggggcaaga agatgcagga cgatctgaac gacgccgtcg
     3841 actgggcggt cggccggggg atcacgactt ccgacaaggt cgcgatcatg ggcggctcct
     3901 atggcggata cgcggtgctc gccggcctcg ccttcacgcc cgacaagttc gcctgcgggg
     3961 tggacatcgt cggcccgtcc aacctcgcca ccctgctcgg caccatcccg ccttattggg
     4021 aggcgctgag ggcgcaattc tacaagagga tgggcgatcc gaacacgccc gagggcaagg
     4081 cctatcagcg ggaagtctcg ccgctctaca gcgccgacaa gatccgccgg ccgctgctca
     4141 tcggccaggg cgccaacgat ccgagggtca atgtcgccga atcgcagcag atcgtcgacg
     4201 ctctcaaggc caagaacatc ccagtcacct acgtcgtctt ccccgacgag ggccacggct
     4261 tcgcccggcc gcagaataat atcgccttca acgcggtggc ggagaatttc ctggccaggt
     4321 gcctgggcgg ccgggccgag ccgatcggcg aggcgctcaa gccgtcgagc gcgcaggtgc
     4381 cgcacggcgc cgagttcgcg ccggggctgg cggaggcttt gaagtagtta atcctccccg
     4441 cgatttcgcg gggaggggga ccaggcgaag cctggtggag gggttcttcg aactgaaagg
     4501 cccctccacc gcgcttcgcg cggtccccct cccctgcgaa ggcaggggag gatttatatt
     4561 cccacgtcct cccact
//
LOCUS       DASWNU010000443         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_199982, whole genome shotgun sequence.
ACCESSION   DASWNU010000443 DASWNU010000000
VERSION     DASWNU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199982"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VGB57_08640"
     CDS             complement(<1..978)
                     /locus_tag="VGB57_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0131461.1"
                     /translation="MTVTIRPVEDAADRKAFVDLAYVLNKDDPNWVPPLKTEVHGLID
                     PRTNPWFEHGDARFFLAERNGEPVGRISAQVDELVIRHMGAGTGQWGMFEAADSEVAA
                     ELIHRAEAWLRGRDMTRSMGPFSLSIWDEPGLLIKGHDHPPMVMMGHNKAEYEAWVEA
                     AGYVKAKDLFTYDLEVTAPFPPIVERIVRAGEKNPRIGIRRTVKKNFEAEARLLLGIL
                     NDAWSDNWGFIPLTEAEIAYAGKKLKPIVLEDMILVAEYDGEPVAFMMALPDINQFLK
                     DLDGSLFPFGWAKLLWRLRRMRPTGGRVPLMGVVKRLHSTRLASQLAFMM"
     gene            1054..1746
                     /locus_tag="VGB57_08645"
     CDS             1054..1746
                     /locus_tag="VGB57_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131462.1"
                     /translation="MKTARDKRRPRWGTLLMEAPAAARLLAAQLRSARVQEDVGGGRA
                     VMVIPAFLANDVPTIQLRRTLRACGFQAYGWKQGFNLGARSGKFERLLRRIDIIAAET
                     RGPVALVGWSLGGLYARELAKRRPGTVSMVVTLGTPFSQGLRSNNAWKLYEAINDHDV
                     DHPPVPIAPDVKPPVRTVAMWSPLDGIVAPASAAGEDGERDRRISLDCRHNEMVSDPG
                     ALRAIVAALAEA"
BASE COUNT          299 a          553 c          648 g          288 t
ORIGIN      
        1 catcatgaag gcgagctggc tcgcgaggcg ggtggagtgg aggcgcttga cgacccccat
       61 cagcgggacc cggccgcccg tcggacgcat ccggcggagc cgccagagca gcttcgccca
      121 gccgaagggg aagaggcttc cgtcgagatc cttgaggaac tggttgatgt cgggaagcgc
      181 catcatgaag gcgaccggct cgccgtcata ttcggcgacg aggatcatgt cctcgaggac
      241 gatcggctta agcttcttgc cggcataagc gatctcggcc tccgtgagcg ggatgaagcc
      301 ccaattgtcc gaccaggcgt cattcaagat gccgaggagc aggcgcgcct ccgcctcgaa
      361 attcttcttc accgtacggc ggatgccgat cctcgggttc ttctcgccgg cccggacgat
      421 gcgctcgacg atgggcggga acggggccgt gacctcgaga tcgtaggtga agagatcctt
      481 ggccttgacg tagccggcgg cctcgaccca ggcttcgtac tcggccttgt tgtggcccat
      541 catcaccatc ggcggatggt cgtggccctt gatcaggagg ccgggctcgt cccagatgga
      601 gaggctgaac gggcccatcg accgggtcat gtcgcggccc ctgagccacg cttcggcgcg
      661 atggatgagc tcggccgcga cctccgagtc ggcggcctcg aacatgcccc actggccggt
      721 gccggccccc atgtgccgga tcacgagctc gtcgacctgg gcggagatcc ggccgaccgg
      781 ctccccgttg cgttcggcca ggaagaagcg ggcgtcgcca tgctcgaacc aggggttggt
      841 gcgcgggtcg atgaggccgt ggacctcggt cttgagcggg gggacccagt tcgggtcgtc
      901 tttgttgagg acgtaagcga ggtcgacgaa ggccttgcgg tcggcggcgt cctcgacggg
      961 gcggatcgtg acggtcatgg gacgaaccta gcgaaccgga ccgggcgacg atagtagctg
     1021 ctgccgcatg gccgcaacgg cggaaggaat gcagtgaaga cggctcgaga taaacgacgt
     1081 ccccgctggg gcacgctcct gatggaggcg ccggccgcgg ctcggctgct ggcggcgcag
     1141 ctcaggagcg cgcgggtaca agaggatgtc ggtggcgggc gggcggtgat ggtcattccg
     1201 gcctttctcg ccaacgacgt gccgacgatt cagctgcggc gaacgctgcg cgcgtgcggc
     1261 ttccaggcct atggatggaa acaggggttc aatctgggcg ctcggagtgg aaagttcgag
     1321 cggctgctga ggcggatcga catcatcgcg gcggaaaccc gagggccggt ggcgctggtc
     1381 ggatggagcc tcggcggtct ttatgcacgg gagctcgcga agaggcggcc cgggacggtg
     1441 tccatggtcg tgaccctcgg aacccctttc tcccagggcc tgcgctccaa caatgcctgg
     1501 aagctctacg aggcgatcaa cgaccacgac gtcgaccacc cgcccgtgcc gatcgctccg
     1561 gacgtgaagc ctccggttcg aaccgtggcc atgtggtcgc cgctggacgg aatcgtcgct
     1621 ccggccagcg ccgcggggga agacggggaa cgggaccggc gcatctcgct cgactgccgg
     1681 cacaatgaga tggtcagcga tccaggcgcg cttcgggcga tcgtcgcggc gctcgcggag
     1741 gcctgaaggg acaagacctg cgccgcgttg cggcaaacgc ctcggcaa
//
LOCUS       DASWNU010000444         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_122072, whole genome shotgun sequence.
ACCESSION   DASWNU010000444 DASWNU010000000
VERSION     DASWNU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122072"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /gene="rplU"
                     /locus_tag="VGB57_08650"
     CDS             complement(<1..412)
                     /gene="rplU"
                     /locus_tag="VGB57_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0131463.1"
                     /translation="MFAVVRTGGKQYRVAAGDKIVVEKLAGEAGDKIQLGDILLAGEG
                     ADLKSTDGLTVSAEIIAQAKADKVIVFKKRRRHNYRRKKGHRQQHTILRITAIGDEKA
                     EQPKKAKAEKPADEASVATAAAPAETPAAAAPTAE"
     gene            complement(616..1002)
                     /gene="hspQ"
                     /locus_tag="VGB57_08655"
     CDS             complement(616..1002)
                     /gene="hspQ"
                     /locus_tag="VGB57_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEY0131464.1"
                     /translation="MMERSDKLFAGNGAAAPLAPPVAHARFAIGDVVRHRLFDFRGVI
                     FDVDPVFANSDEWYEAIPEDIRPPKDQPFYHLLAENGESSYTAYVSQQNLLADESDEP
                     VDHPAISGLFDTISRGRYRLKREHRH"
     gene            1139..1228
                     /locus_tag="VGB57_08660"
     tRNA            1139..1228
                     /locus_tag="VGB57_08660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1173..1175,aa:Ser,seq:cga)
     gene            complement(1282..1845)
                     /locus_tag="VGB57_08665"
     CDS             complement(1282..1845)
                     /locus_tag="VGB57_08665"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0131465.1"
                     /translation="MSPRAKCAASTRDTMLASARRRFLEESYENVGLRDIARDVGVDV
                     ALVGRYFGSKENLFHEVLRGMRCDWLDAEISAEELPAFLAAMVLRKEEAEDREDLDRL
                     LIILRSASSPKASELVRIAFREDVIEPVARLLPGPDAERRVAVAFSILTGTTVMRTFM
                     DVEPLLACCQHGLEQKLTRLLREALSN"
     gene            2020..3216
                     /locus_tag="VGB57_08670"
     CDS             2020..3216
                     /locus_tag="VGB57_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0131466.1"
                     /translation="MPAIRSALRGLLPFLLALSLAGCGDAEGQAGPPPAMPVTVAAPL
                     VQDVVDWDEFVGRFEAIQSVEVKPRATGFLQAVHFQDGQYVRAGQLLFTIDPRQSQAA
                     LAQAQAQLARAQATLANARTELARSRALAAQQAASTEEVEQRQAAVRAGEADIAAARA
                     AIRSQQLNVGFTRVTAPISGRVSERRVDPGNSVSADQTVLTTIVSTSPLHFAFEGSEA
                     LLLKYQRQNSGARAGTPIRIRLQDEATYVHAGSLDFVDTVVNTGAGTVRARAVVANPA
                     GFLKPGMFGHARLASSQPYRAMLVPDSAIVTDAARRVVYVVSPQGLVLARPVVLGPLL
                     GNLRVVRSGIAPQDRVIINGIQRARPGQKVVPKPGRIAPGGGPEPLPAAPASPPSSIA
                     TPAGAL"
     gene            3235..>3570
                     /locus_tag="VGB57_08675"
     CDS             3235..>3570
                     /locus_tag="VGB57_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0131467.1"
                     /translation="MNISRFFIERPIFAAVIAIFITLIGAFAYPQLSLSQYPEIAPPT
                     ITINTAYPGASSETMAETVAAPIEQEVNGVEGMLYMSSSSTNGAAGITVTFEPGTDLD
                     AAQVLVQNRV"
BASE COUNT          542 a         1226 c         1205 g          597 t
ORIGIN      
        1 cctcggcggt cggggcggca gcggcgggag tctcggccgg agcggcagcg gtggcgacgg
       61 aggcctcgtc ggcgggcttt tcagccttcg ccttcttcgg ctgctcggcc ttctcgtcgc
      121 cgatcgcggt gatcctcagg atcgtgtgct gctggcgatg gcccttcttg cgacggtaat
      181 tgtgccggcg gcgcttcttg aagacgatga ccttgtcggc cttggcctgg gcgatgatct
      241 ccgccgagac ggtgagcccg tccgtcgact tgaggtcggc accctctccg gcgagcagga
      301 tgtcgcccag ctggatttta tcccccgcct ctccggcgag cttctcgacg acgatcttgt
      361 ctccggcggc gacgcgatac tgcttgccgc ccgtgcgcac gactgcgaac atggcttttt
      421 ccatcatact aatgctgtcc gcacaaatcg tcggctgcgg ccgaaccccc gcgcgggaac
      481 cgggctgcta ccgaagccgg cccggagtgt caacgtctct cgtcaccccg gcggagggcc
      541 ggggtcccag catagaccgg catagagcct ttcacccgct ggaccccggc tttcgccggg
      601 atgacggctt tgccgtcagt gccgatgctc gcgcttcagg cggtaccggc ccctggagat
      661 agtgtcgaag aggccggata tggccgggtg gtcgacgggc tcgtcgctct cgtcggcgag
      721 cagattctgc tggctgacgt aggcggtgta ggaggattcg ccgttctcgg cgagcagatg
      781 gtagaagggc tggtccttgg gcgggcgaat gtcctccggg atcgcttcat accattcgtc
      841 tgagttggcg aagaccgggt cgacgtcgaa gatgactccg cggaagtcga acaaccggtg
      901 ccggaccacg tcgccgatgg cgaagcgggc atgcgccacc ggcggcgcca gcggcgcggc
      961 cgctccgttg ccggcgaata atttgtcgga ccgttccatc ataaccccta tgtaaggcct
     1021 ggcgggcgac aggcaagcgg ggtgagggag tccgacacct tggcaagccg gaacgcttgg
     1081 gctaagcgcc cgccctgtcg accgggtccc gcttgccagc ggggcgaatc ctcaatgcgg
     1141 agaggtgccg gagtggtcga tcggggcggt ctcgaaaacc gttgagcccg taagggttcc
     1201 gagggttcga atccctccct ctccgccatt ttcttcatcg gcccagactg gaaacgcgcc
     1261 cggcggatcg cgggctgtcg ctcaattcga gagggcttct ctcagcagcc gggtgagctt
     1321 ctgctcgagg ccgtgctggc agcacgccag taggggctcg acgtccatga aggtgcgcat
     1381 gacggtggtt ccggtcagga tactgaacgc gacggcgacc cgccgttcgg cgtccgggcc
     1441 gggaaggagg cgcgcgaccg gctcgatcac gtcctcgcgg aaggcgatcc tgaccagctc
     1501 ggaggctttc ggcgaggagg ccgagcgaag gatgatcaat aggcggtcga gatcctcgcg
     1561 gtcttccgct tcctccttgc gaagaaccat cgcggcgagg aaggcgggga gctcctcggc
     1621 ggagatttcg gcgtcgagcc agtcgcagcg cattccgcgc aggacctcgt ggaagagatt
     1681 ctccttcgat ccgaaatagc ggccgaccag ggcgacatcg accccgacgt cgcgggcgat
     1741 gtcgcggagc ccgacattct cgtagctctc ctcgaggaat cggcggcgcg cggaggcgag
     1801 catcgtgtcg cgggtggaag cggcgcattt cgcgcggggg gacattctta cactccttgg
     1861 tctcaggcgg aattgtccgg aacggatgca ttgtcaacaa gtgtagacaa aatatgtcca
     1921 cagccgttga catagcctga acggctactt aggtcccctc ccgccgggcg gccgaggagc
     1981 ggtggcccag cggatgacac cccgcccgaa agcttgaaga tgcccgcaat ccgatccgcc
     2041 ctgcgcggcc tgctcccgtt cctcctcgcg ctctctctcg ccggttgcgg cgatgccgag
     2101 ggccaggcgg ggccgccgcc ggcgatgccg gtgacggtcg ccgcgccgct cgtccaggac
     2161 gtcgtcgact gggacgagtt cgtcggccgg ttcgaagcga tccagagcgt cgaggtgaag
     2221 ccgcgcgcga ccggcttcct ccaggcggtg cacttccagg acggccaata tgtccgggcc
     2281 gggcagcttc tcttcacgat cgacccgagg cagtcccagg ccgctctcgc ccaggcgcag
     2341 gcccagctcg cccgggccca ggcgaccctc gccaatgccc ggaccgagct cgcccgctcg
     2401 cgtgccctgg cggcccagca ggcggcgagc accgaggagg tcgagcagcg ccaggccgcc
     2461 gtccgcgccg gcgaggccga catcgccgcg gcgcgggccg cgatccgaag ccagcagctc
     2521 aacgtcggct tcacccgggt gacggcgccg atctctggcc gggtgtcgga gcgccgggtc
     2581 gatccgggca acagcgtctc ggccgaccag acggtgctca ccactatcgt ctcgaccagc
     2641 ccgctccact tcgccttcga gggatcggaa gccctgcttc tcaaatatca gcgccagaat
     2701 tcgggcgccc gcgccggcac cccgatccgg atccgcctcc aggacgaggc cacctacgtc
     2761 catgccggga gcctcgattt cgtcgatacg gtggtgaaca ccggcgccgg caccgtccgc
     2821 gcccgggcgg tcgtcgccaa tcccgccggt ttcctcaagc ccgggatgtt cggccacgcg
     2881 cggctcgctt cgtcgcagcc ctaccgggcg atgctggtcc ccgacagcgc gatcgtcacc
     2941 gatgcggcgc gccgggtcgt ctacgtcgtg agcccgcagg ggctggtgct ggcccggccg
     3001 gtggtgctcg ggccgctgct cggcaacctt cgggtggtgc gcagcgggat cgcgccgcag
     3061 gaccgggtga tcatcaacgg cattcagcgc gcccggcccg gccagaaggt cgttcccaag
     3121 cccggccgga tagcgcccgg aggcggcccc gagccgctgc ccgccgcccc cgcctcgccc
     3181 ccgtcgtcga tcgcgacgcc ggccggcgcc ctctaaggcg aagcgacaga ccccatgaac
     3241 atttcgcgct tcttcatcga gcggccgatc ttcgccgcgg tgatcgcaat cttcatcacc
     3301 ctgatcggcg ccttcgctta tccgcagctc tcgctctcgc aatatcccga gatcgcgccg
     3361 ccgacgatca ccatcaacac cgcctatccg ggcgcgtcat ccgagacgat ggccgagacg
     3421 gtggccgcgc cgatcgagca ggaggtgaac ggggtcgagg gcatgctcta catgtcctcc
     3481 tcttccacca acggagcggc ggggatcacc gtcaccttcg agccgggcac cgatctcgac
     3541 gccgcccagg tgctggtcca gaaccgggtg
//
LOCUS       DASWNU010000445         6837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_122187, whole genome shotgun sequence.
ACCESSION   DASWNU010000445 DASWNU010000000
VERSION     DASWNU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122187"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1666
                     /locus_tag="VGB57_08680"
     CDS             71..1666
                     /locus_tag="VGB57_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0131468.1"
                     /translation="MATAVSGDYVIDAKRDRLVIFASTLGTVFEWYDFFIYGTLAAVI
                     GKQFFPAQSETAQFLLALASFGVGFGVRPLGAVLFGYLGDRLGRKYTFLVTITLMGVA
                     TAAVGLLPGYAAIGMAAPALLVFLRVLQGLALGGEYGGAAIYVAEHAPRHKRGFYTSF
                     IQAGVIGGFLLSVAVVLVSELFVDKGEWEAWGWRIPFLFSVVLLAISLWVRLKLKESP
                     VFQAMKDKGEIARNPLKETFASRSNVKLVLVALFGIAAGLTVIWYTAQFQAMYFLQNS
                     LRIEDTAARAMIGAAAAFSMLWFILFGWLSDRVGRKGPIVIGYALTIVLMFPLFHWMA
                     SAANPELSAAMRKNPVVVTGSDCTYAPFAKVQPTQCGKLLDALSKKGIAYSTVEAPAG
                     ASPGVTIGGVAVDASDPASLDFGLRIAGYKLEKSAPTFAQALQVILAIDLIGLCSGMT
                     FGPVAALLVELFPARIRYTSMSIPYHIGTGYFGGFLPFISQYIVAKTGDPFAGLWYTI
                     GVVAMALVVTLFWLPETAGKELE"
     gene            1794..2840
                     /locus_tag="VGB57_08685"
     CDS             1794..2840
                     /locus_tag="VGB57_08685"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0131469.1"
                     /translation="MPEPHSPLRLLLDGEALVENWRWLRRQSAGADCGAAVKADGYGL
                     GAREIVIRLANEGCRDFFVATWREAEALLEALGPASVSVLHGLAGEDLAHAAGSPARP
                     VLNTPLQVERWKSQGRGRPCDVMIDTGINRLGLSPAQVGEGILDGLEIDILMSHLASA
                     DEDVAMNERQRADFAGLAARVPAKRRSLANSAGICLGADYAFDLTRPGLALYGGIPRR
                     EAAGHIRQVVRVEAQIIQRRRVEAGGSVGYNSTWRATAPAELAILNLGYADGYLRGFS
                     GTGRARLPSGSAPVVGRVSMDLTAILVDEAPELEEGDWVELDYGLPSASAQSGLSQYE
                     LLTTLGGRHARVWK"
     gene            2958..3851
                     /locus_tag="VGB57_08690"
     CDS             2958..3851
                     /locus_tag="VGB57_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0131470.1"
                     /translation="MARIAHLSDVHFGCQDDKVVDGANAWLQEQQPDLIIVSGDFTQR
                     ARVEQFKQASTWLNKLRAAGWPVLAVPGNHDIPLYDVATRFAAPLNRYKKYISNDLCP
                     WFENDEVAVLGINTARSLTIKDGRINRDQMKIIEERFAAVAPGKTKILVTHHPLFSMP
                     IGEGTELTEAVGRHEDAVTAVCRAGVNIALAGHFHRTYAEAARKMVEKAGGALVIQAG
                     TATSKRLRNNELQSFNWIHAHRDNDVELQVIAWDGSGFRRGSHVRYVYDGENWNSKTV
                     PHDSPKESAAGQMAALPGAAA"
     gene            complement(3989..4978)
                     /locus_tag="VGB57_08695"
     CDS             complement(3989..4978)
                     /locus_tag="VGB57_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0131471.1"
                     /translation="MAPSGTIDRVEALLAARRFDEAARLLLAAAGAGETAAVAELAHW
                     RIVGNLIRRDLPEARRLLGVAAAKGDDNSAFLHAGFLAGGFGGADDWPGALAALRGLA
                     KRRPRAAAQLRLIEAMELDGDGFPARPIETRRLGETPYVVAAPAFMTEAECLYLRNEG
                     EPDLQTSAVVDPATGRMVPHPVRSADSAAFGVYAEDLVVNALNRRIAALSGTRLDQAE
                     PLQLLRYRPGGEYKPHMDALPAEANQRVLTVLVYLSDDYEGGETRFPHTGLSFRGRAG
                     DALMFRNAGADGRPDPLSLHAGQPVTKGTKYLASRWIRAARFTFPPPRPILDL"
     gene            5105..5956
                     /locus_tag="VGB57_08700"
     CDS             5105..5956
                     /locus_tag="VGB57_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0131472.1"
                     /translation="MTIVKGLTRASALALGLLAAVSCAPSPRAVAAAEADLHADTAIA
                     AAVGSPTRTEANRARDAWRHPRETLGFFGVEPDDTIVEIWPGGGWYTEILAPLVRENG
                     TYYAAANGNGLNGAKAMLARDPALYGSMRLASFPTGRPGADQPAVPAGTADVVLTFRN
                     VHNWIMPDQPFAEEAFRQMFAMLKPGGTLGVVEHRLPESADPAREKSTGYIKVSTVRR
                     LAETAGFRLVASSEINANPRDTKDHPGGVWSLPPSLRGGDKDRERFLAIGESDRMTLR
                     FVKPGRP"
     gene            6070..6294
                     /locus_tag="VGB57_08705"
     CDS             6070..6294
                     /locus_tag="VGB57_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131473.1"
                     /translation="MTDSQILTLNERVKGISGYLFNLSAVLVATAVARMWVKGDVDFA
                     ALIWVAGGAILLWLGWSILYLLEPENEEKA"
     gene            6294..6449
                     /locus_tag="VGB57_08710"
     CDS             6294..6449
                     /locus_tag="VGB57_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131474.1"
                     /translation="MHTLSDWAPLIGAGFVAMNALFLLWLSVRQAHSGERKAAKAARE
                     AGRHGKA"
     gene            complement(6464..>6837)
                     /locus_tag="VGB57_08715"
     CDS             complement(6464..>6837)
                     /locus_tag="VGB57_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0131475.1"
                     /translation="NAYSAEVLPKIKWRISITGRNVARAHPAGVKIAFGTDAGVSKHG
                     RNADEFELMVKHGMPAAAAIQAATVNAADLLGLASEVGSVEPGKSADLIAVEGDPLAD
                     VTVLKRVGFVMARGRVVKEER"
BASE COUNT         1076 a         2323 c         2315 g         1123 t
ORIGIN      
        1 aggaattaga gccgcttgcc cgccccctcc cgccccgatt acacaggcgc aggcagggca
       61 gggggcttga atggcgacgg cggtatccgg cgattatgtg atcgacgcga agcgggaccg
      121 gctggtcatc ttcgcctcga cgctcggcac agtcttcgaa tggtatgatt tcttcatcta
      181 cgggaccctc gccgcggtca tcggcaagca attcttcccg gcccagagcg agaccgccca
      241 gttcctgctc gccctcgcca gcttcggagt cggcttcgga gtccgtccgc tcggagcggt
      301 cctgttcggc tatctcggcg acagattggg gcgcaaatac accttcctcg tcaccatcac
      361 cctgatgggc gtggccaccg ccgcggtcgg cctgctgccc ggctatgccg cgatcggaat
      421 ggcggcgccc gctttgctcg tcttcctgag ggtgttgcag gggctggcgc tgggcgggga
      481 atatggcggc gcggcgatct acgtcgccga gcatgcgccg cgccacaagc gcggcttcta
      541 caccagcttc atccaggccg gagtgatcgg cggcttcctg ctctcggtcg ccgtcgtcct
      601 ggtctccgag ctgttcgtcg acaagggcga gtgggaagcg tggggctggc gcattccctt
      661 cctcttctcg gtcgtgcttc tcgccatctc cttgtgggtt cgcctgaagc tcaaggagag
      721 cccggtcttc caggcgatga aggataaagg cgagatcgcc aggaatccgc tcaaggagac
      781 cttcgcgagc cgctccaacg tcaagctcgt cctcgtcgct ttgttcggga tcgccgccgg
      841 cctcaccgtc atctggtaca ccgcccagtt ccaggcgatg tacttcctcc agaacagcct
      901 gaggatcgag gacaccgccg cccgtgcgat gatcggcgcc gccgcggcct tttcgatgct
      961 gtggttcatc ctgttcggct ggctgtccga ccgggtcggc cggaaggggc cgatcgtgat
     1021 cggctacgcg ctcaccatcg tcctcatgtt ccccttgttc cactggatgg cctcggccgc
     1081 caatccggag ctgtcggcgg cgatgcgcaa gaatcccgtg gtcgtcaccg gcagtgactg
     1141 cacctacgcg cccttcgcca aggtccagcc gacgcagtgc ggcaaattgc tcgacgccct
     1201 gtcgaagaag ggcatcgcct attcgaccgt cgaggcgccc gcgggcgctt cgccgggagt
     1261 gacgatcggc ggagtcgccg tcgacgcgtc cgatccggcg tcgctcgatt tcggcctcag
     1321 gatcgccggc tacaagctgg agaaaagcgc gcccaccttc gcccaggcgc tccaggtgat
     1381 cctcgccatc gacctcatcg gcctgtgctc cgggatgacc ttcggtccgg tggcggccct
     1441 gctcgtcgag cttttcccgg cgcggatccg ctacacgtcc atgtcgatcc cctatcacat
     1501 aggcaccggc tatttcggcg gcttcctgcc cttcatcagc cagtatatcg tcgccaagac
     1561 cggcgatccg ttcgcgggcc tgtggtacac gatcggagtg gtggcgatgg cgctggtggt
     1621 gacgttgttc tggcttcccg agacggcggg gaaggagctg gaatgacgcg tcgccccagc
     1681 gaaggctggg gccccggcgg gtgaaaagcg cgggcgcctt tcaccccccg ggatcccagc
     1741 cttcgccggg atgacgggtt gctccggcct cgcccgggac gtatccgcct ctcgtgcccg
     1801 agccccattc cccgcttcgc ctgctgctcg acggcgaggc cctggtcgag aactggcgct
     1861 ggctgcggcg gcaaagcgcc ggcgccgact gcggggcggc ggtcaaggcc gacggttacg
     1921 gcctcggcgc ccgcgaaatc gtcattcggc tcgccaacga aggctgccgc gacttcttcg
     1981 tcgccacctg gcgcgaggcg gaggccttgc tcgaagcgtt ggggccggcg tccgtctcgg
     2041 tgcttcacgg cctcgccggg gaagaccttg cccacgccgc cggctcgccc gcccgtccgg
     2101 tgctcaacac cccgctccag gtcgagcgct ggaagtcgca gggaaggggg cgtccctgcg
     2161 acgtcatgat cgacaccgga atcaaccgcc tcgggctaag cccggcgcag gtgggggagg
     2221 ggatactcga cgggctcgag atcgacatat tgatgagcca cctcgcctcg gccgacgagg
     2281 atgtggcgat gaacgagcgc cagcgcgccg acttcgccgg gctcgccgcc cgggtgccgg
     2341 cgaagcgccg aagcctcgcc aacagcgcgg ggatttgcct gggcgccgat tacgccttcg
     2401 atctcacccg gcccggcctc gccctctacg gcggaatccc gaggcgcgag gcggcgggcc
     2461 atatccgcca ggtggtccgg gtcgaggcgc agatcatcca gcgccgcagg gtcgaggcgg
     2521 gcggctcggt cggctacaat tcgacctggc gggcgaccgc gccggccgag ctcgccatcc
     2581 tcaacctcgg ctatgccgac ggctatctgc gcggcttctc cggaaccggc cgggcgcgac
     2641 tgccgagcgg ctccgcgccc gtcgtcggcc gggtctcgat ggacctcacc gccatcctcg
     2701 tcgacgaggc gcccgagctg gaggaaggcg attgggtcga gctcgattac ggccttccgt
     2761 cggcgtcggc gcagtcgggt ttgtcgcaat atgaattgct gacgaccttg gggggaaggc
     2821 atgcgcgggt ctggaagtaa ccggtcattg cgaggagcga agcgacgaag caatccaatc
     2881 gcgggctccg agccgccgga ttgcctcgct ttgctcgcaa tgacgaaact ctgacgaagg
     2941 aggcgggttc accccccatg gcccgtatcg cgcacctctc cgacgtccat ttcggctgcc
     3001 aggacgacaa ggtcgtcgac ggcgccaacg cctggctcca ggagcagcag ccggatctga
     3061 tcatcgtctc gggcgacttc acccagcggg cccgggtcga gcagttcaag caggcctcga
     3121 cctggctcaa caagctgagg gccgcgggct ggcccgtgct cgcggtcccc ggcaatcacg
     3181 acatcccgct ctacgacgtc gccacccgct tcgccgcccc gctcaatcgc tacaagaaat
     3241 atatctccaa cgatctgtgc ccctggttcg agaatgacga ggtggcggtg ctcggcataa
     3301 acaccgcccg ctcgctcacc atcaaggacg gccggatcaa ccgcgaccag atgaagatca
     3361 tcgaggagcg tttcgccgcg gtggcgcccg gcaagacgaa gatcctagtc acccatcacc
     3421 cgctcttctc gatgccgatc ggcgagggga cggagctgac cgaggcggtc ggccggcacg
     3481 aggacgcggt gacggcggtg tgccgggccg gggtcaacat cgctctcgcc ggccatttcc
     3541 accggaccta tgccgaagcg gcgcgcaaga tggtcgagaa agccggaggc gccctcgtca
     3601 tccaggccgg cacggcgacc tcgaagcggc ttcgcaacaa cgagctgcag agcttcaact
     3661 ggatccacgc ccatcgcgac aacgacgtcg agctccaggt gatcgcctgg gacggcagcg
     3721 gctttcgccg cggaagccac gtccgctacg tctatgacgg cgagaactgg aattcgaaga
     3781 cggtcccgca cgacagcccg aaggagtcgg cggcggggca gatggcggct ttgccggggg
     3841 cggcggcgta accaaatcct cccctgcctt tgcaggggag gggcaccacc cgaagggtgg
     3901 tggaggggcc ttgctgagtt agaagaaccc ctccaccgcg cttcgcgcgg tccccctccc
     3961 cgcgaaatcg cggggagggt ttttgtctct acaggtccaa aatcggccgc ggcggcggga
     4021 aagtgaacct ggccgctcga atccagcgcg acgccagata tttggtcccc ttggtgaccg
     4081 gctgcccggc gtggaggctg agcgggtcgg ggcggccgtc cgctccggcg ttgcggaaca
     4141 tcagtgcgtc gcccgcccgg ccgcggaagg acaggccggt gtgcgggaag cgcgtctcgc
     4201 cgccttcata atcgtcggaa agatagacca gcacggtcag gacgcgctgg ttggcctcgg
     4261 ccggaagcgc gtccatgtgg ggcttatatt cgccgcccgg ccgatagcga agcagctgca
     4321 gcggctcggc ctggtcgagc cgggtcccgc tcagcgccgc gatcctccgg ttgagcgcgt
     4381 tgactacgag gtcctcggca tagaccccga acgccgccga gtcggccgag cgcaccggat
     4441 gcggcaccat tcgcccggtg gccggatcga cgaccgccga ggtctgcagg tccggctccc
     4501 cctcattcct caggtagagg cactcggcct cggtcatgaa tgccggggcg gcgacgacat
     4561 aaggcgtctc gccgaggcgc cgggtctcga tcggccgggc cggaaagccg tcgccgtcca
     4621 gctccatcgc ctcgatcagt cgaagctgcg ccgccgctcg aggccgacgc ttcgccagcc
     4681 ctcgaagcgc cgcgagggcg ccgggccagt cgtcggcacc gccgaagccg ccggcgagga
     4741 agccggcgtg gaggaaggcc gaattgtcgt cgcctttggc ggccgcgact ccgagcagcc
     4801 gcctcgcttc ggggagatcg cggcggatga ggttgccgac gatccgccaa tgggcgagct
     4861 cggccaccgc cgcggtctcg ccggcgccgg ccgcggcgag gagcagccgc gccgcttcgt
     4921 caaagcgccg agccgcgagc agcgcctcca cccgatcgat cgttccactg ggagccatgc
     4981 gcgttcccta ggcgcgcgcg ccggataaaa aaagcgcgag gcttcgcggg agcggacgag
     5041 ttgcgcggcc gtctccgctc cgccatgagc ggccgggcag gatgtggaaa aatcggagag
     5101 agcaatgacc atcgtaaaag gcctcaccag ggcgagcgcc ctcgcgctcg ggctcctcgc
     5161 cgcagtctcc tgcgcgcctt cgccgagagc ggtcgcggcg gccgaggccg atctccatgc
     5221 cgacacggcg atcgcggcgg cggtcggctc gccgacccga accgaagcga accgggcccg
     5281 cgacgcctgg cgccacccgc gcgagacgct cggcttcttc ggagtggagc ccgacgacac
     5341 gatcgtcgag atctggcccg gcggcggctg gtataccgag atactggcgc cgctggtgcg
     5401 cgagaacggc acttattatg ccgcggccaa cggcaacggc ctgaatggcg ccaaggcgat
     5461 gctggcaagg gatccagccc tctacggcag catgcgcctg gcctccttcc ccaccggccg
     5521 cccgggcgcg gaccagccgg cggtgccggc cgggacggcc gatgtcgtgc tcaccttccg
     5581 caacgtccac aattggatca tgccggacca gcccttcgcc gaggaggcgt tccggcagat
     5641 gttcgcgatg ctgaagccgg gcgggaccct gggcgtggtc gagcaccggc ttccggaaag
     5701 cgcggatccg gcgcgcgaga agtcgaccgg ctacatcaag gtctcgacgg ttcggcgcct
     5761 ggccgagacg gccggcttcc gcctcgtcgc ttcgagcgag atcaacgcca atccgcgcga
     5821 taccaaggac catccgggcg gagtctggtc gcttccgccg agccttcgag gcggcgacaa
     5881 ggatcgcgag cgcttcctcg ccatcggcga gagcgaccgg atgactttga ggttcgtcaa
     5941 gccggggcga ccttaggacg agaagtctcg gcgggtctgc ccgaggtccc ggctttcgcc
     6001 gggatgacgg ggaggggagc cagcctatcc gaattttagg tgtacggaac gccgggctag
     6061 accggcgtca tgaccgattc gcagatcctg acgctgaacg agcgggtgaa agggatttcg
     6121 ggctatttgt tcaacctgtc ggcggtactg gtcgcaactg cggtggcccg catgtgggtg
     6181 aagggcgatg tggatttcgc ggcattgata tgggtcgcag gcggggccat cttgctgtgg
     6241 ctgggctgga gcatattgta cctgctcgaa cccgagaatg aggagaaggc ctgatgcaca
     6301 cgctcagcga ctgggcacct cttatcggtg ccggctttgt cgccatgaat gccctcttcc
     6361 tgctctggct ctcggtccgt caggctcatt cgggcgagcg caaggcggcc aaggcggcac
     6421 gcgaggctgg acggcacggc aaggcctaag agagccagct gacctacctc tcctccttca
     6481 ccacccgccc gcgcgccatg acgaagccga cccgcttcag caccgtcacg tcggcgagcg
     6541 ggtcgccctc cacggcgatc aggtcggcgc tcttgccggg ctcgacggac ccgacttccg
     6601 aagcgaggcc gagcaggtcg gcggcgttga cggtggcggc ctggatggcc gcggcggcgg
     6661 gcattccgtg cttcaccatc agctcgaact cgtcggcgtt gcggccgtgc ttcgacactc
     6721 cggcgtcggt tccgaaggcg atcttcacgc ccgccggatg cgccctggcg acgttccggc
     6781 cggtgatcga tatccgccat ttgatcttgg gcaggacctc cgccgaatag gcgtttg
//
LOCUS       DASWNU010000446         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_327781, whole genome shotgun sequence.
ACCESSION   DASWNU010000446 DASWNU010000000
VERSION     DASWNU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327781"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGB57_08720"
     CDS             <1..435
                     /locus_tag="VGB57_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0131476.1"
                     /translation="VYVGAVAVLFLFVVMMLDVDFATLRAGVARYWPFGFGLALVLLV
                     EIFFAAMAWNAGALGAGVRGAPTDPNVPNIEALGRVLYTRYLYVFEGAGLVLLVAMIG
                     AIVLTHRTRSGLRPQNVSDQNRRRPEDAVVNMRPGVGEGMKL"
     gene            530..835
                     /gene="nuoK"
                     /locus_tag="VGB57_08725"
     CDS             530..835
                     /gene="nuoK"
                     /locus_tag="VGB57_08725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY0131477.1"
                     /translation="MIGLGHYLAVSAILFVLGVFGIFLNRKNIIVILMAIELILLAVN
                     INLVAFSAYLGDMVGQIFAMFVLTVAAGEAAIGLAILVIYFRGRGTIAVDDINQMKG"
     gene            951..>2020
                     /gene="nuoL"
                     /locus_tag="VGB57_08730"
     CDS             951..>2020
                     /gene="nuoL"
                     /locus_tag="VGB57_08730"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0131478.1"
                     /translation="MTVQLIVFLPLLAAIVAGLGNRMLGNTVARLITTGALFAACALS
                     WPIFLGFVAGHGESQLYPVLSWIHSGTMRVDWALRVDALTSVMLVVVTTVSALVHLYS
                     WGYMAEDDSQPRFFAYLSLFTFAMLMLVTADNLVQMFFGWEGVGLASYLLIGFWYHKP
                     SANAAALKAFVVNRVGDFGFSLGIFATFLVFGTVSIPEILAASPGMAGSTVGFLGQRF
                     DTMTLICILLFIGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVCRLS
                     PMFETSETALTVVTVIGAATAIFAATVGTVQNDIKRVIAYSTCSQLGYMFFAAGVGAY
                     GAAMFHLFTHAFFKALLFLGAG"
BASE COUNT          283 a          697 c          636 g          404 t
ORIGIN      
        1 gtctacgtcg gagcggtcgc ggtgctgttc ctgttcgtgg tgatgatgct ggacgtcgat
       61 ttcgccaccc tgcgcgccgg agtggcgcgc tactggccgt tcggtttcgg cctcgccctg
      121 gtccttctcg tcgagatatt cttcgccgcc atggcgtgga acgcgggagc gctcggcgcc
      181 ggcgtccggg gcgctccgac cgatccgaac gtccccaaca tcgaggcgct ggggcgggtc
      241 ctctacaccc gctacctcta cgtcttcgaa ggcgccgggc tggtcctgct ggtggcgatg
      301 atcggagcga tcgtcctcac ccaccgcacc cgcagcgggt tgaggccgca gaacgtgagc
      361 gaccagaacc gccgccggcc cgaagatgcg gtggtcaaca tgcggccggg cgtgggtgag
      421 gggatgaagc tgtgagccag ttcgtcatcc cggcgaaagc cgggacctca ggtcggaaag
      481 tctcgacggg tctccccgag gccccggcgt tcgccggggt gacgggatca tgatcggcct
      541 tggccattac ctcgccgtca gcgcgatcct gttcgtgctc ggggtgttcg gaatcttcct
      601 caaccgcaag aacatcatcg tcatcctgat ggcgatcgag ctcatcctgc tcgcggtcaa
      661 catcaacctc gtcgccttct ccgcctatct cggcgacatg gtcggccaga tcttcgccat
      721 gttcgtgctc accgtcgccg ccggcgaagc ggcgatcggc ctcgccatcc tcgtcatcta
      781 cttccgaggc cgcggcacga tcgcggtcga cgacatcaac cagatgaagg ggtgaccgct
      841 ttgccgccgt cattgcgagg agcgtcagcg acgaagcaac ccagtgggcg gatgcgccgc
      901 actggattgc ttcgcttcgc tcgcaatgac ggggaggggg ccgcgacgtc atgaccgtcc
      961 agctcatcgt cttcctgccg ctgctggcgg cgatcgtcgc cgggctcggc aaccggatgc
     1021 tcggcaacac cgtcgccagg ctgatcacca cgggcgcgct cttcgccgcc tgcgccctgt
     1081 cctggccgat cttcctcggc ttcgtggcag gccacggcga aagtcagctc tacccggtcc
     1141 tgtcgtggat ccattccggg acgatgaggg tcgactgggc gctgagagtc gacgctctga
     1201 cctcggtgat gctggtggtg gtgacgacgg tctccgcgct cgtccacctc tactcctggg
     1261 gctatatggc ggaggacgac agccagcccc gcttcttcgc ctatctgtcg ctcttcacat
     1321 tcgcgatgct catgctggtg acggccgaca acctcgtcca gatgttcttc ggctgggagg
     1381 gggtcggcct cgcttcctat ctcctcatcg gcttctggta ccacaagcct tcggccaatg
     1441 ccgcggcgct caaggccttc gtcgtcaacc gggtcggcga tttcggcttc agcctgggaa
     1501 tcttcgccac cttcctggtc ttcggcacgg tctccatccc cgagatcctc gccgcctcgc
     1561 ccggaatggc gggctcgacc gtcggcttcc tcggccagcg cttcgacacg atgacgctca
     1621 tctgcatcct cctgttcatc ggagcgatgg gcaaatcggc gcagctcggc ctccacacct
     1681 ggcttcccga cgcgatggag ggcccgaccc cggtctccgc cctcatccac gccgcgacga
     1741 tggtcacggc gggcgtgttc atggtctgcc gcctctcgcc catgttcgag accagcgaga
     1801 ccgcgctgac cgtcgtcacc gtgatcggcg cggcgacggc gatcttcgcg gccaccgtcg
     1861 gcacggtgca gaacgatatc aagcgggtga tcgcctattc gacctgctcg cagctgggct
     1921 acatgttctt cgcggcgggc gtgggcgcct acggggcggc gatgttccac ctgttcaccc
     1981 atgccttctt caaggccctg ctgttcctgg gcgccggcag
//
LOCUS       DASWNU010000447         4842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_172004, whole genome shotgun sequence.
ACCESSION   DASWNU010000447 DASWNU010000000
VERSION     DASWNU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172004"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VGB57_08735"
     CDS             complement(<1..254)
                     /locus_tag="VGB57_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0131479.1"
                     /translation="MPRQITAFSNPLVKDVRALREKKGRRRQGMFLAEGLRILTEARE
                     AGRLPAILFFAAGPSHPLLETLIAEVEAAHGDVIETNADIL"
     gene            complement(337..609)
                     /locus_tag="VGB57_08740"
     CDS             complement(337..609)
                     /locus_tag="VGB57_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY0131480.1"
                     /translation="MSVASRTVEMRNKRGLHARASARFVTMAAGLPAGVEVEKDGSRV
                     CGTSIMGLMMLGAAFGDCVTIHTEGEGAEEALEKLVALVEDRFGEE"
     gene            complement(606..1010)
                     /locus_tag="VGB57_08745"
     CDS             complement(606..1010)
                     /locus_tag="VGB57_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0131481.1"
                     /translation="MIGLVLVTHGRLAAEFVRAMEHVVGPQERIEAICIGPEDDMEGR
                     RNDIAEAIAKVDDGSGVIILTDLFGGTPSNLAISLMKSEKIEVIAGVNLPMLIRLEGA
                     RKLLDVKAAVAAAREAGRKYISVASEILGETV"
     gene            complement(1010..1948)
                     /gene="rapZ"
                     /locus_tag="VGB57_08750"
     CDS             complement(1010..1948)
                     /gene="rapZ"
                     /locus_tag="VGB57_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0131482.1"
                     /translation="MTATAAPQRILLVTGLSGAGKSTALRTLEDLGWEVVDNLPLSLL
                     EDLLETPLGKGNSRRRRPLAIGVDSRTRDFDAKRIVQQIKALSSEQRVPVETLYFDCA
                     GSELLRRYSETRRRHPLAPDRPATDGIAAERELMEPLRRWADHVVDTTDTAPNALQQQ
                     IRQRFSSGAIAPTLSVMSFGFARGIPRNADNVFDLRFLRNPYWVDSLRDLTGLDPAVG
                     DYISGDEAYGEALTKIEDLILTLLPRYGAEGKSYVTMAFGCTGGRHRSVHVAERVAAR
                     LREAGFSPTVEHRDLASLPREGVEGGGGGKPQDWEV"
     gene            complement(1945..2376)
                     /locus_tag="VGB57_08755"
     CDS             complement(1945..2376)
                     /locus_tag="VGB57_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0131483.1"
                     /translation="MSLLADETVHASCVAIGGRGILLAGRSGAGKSDLALRLIDRGAA
                     LVSDDYTELRCVGGTLLARAPATIVGKIELRGVGIIALEPAADVPVCLYADLGSTPER
                     LPEPATIGLAGVDIPFVALAALEPSAALKLEYALVHFGLPL"
     gene            complement(2373..3881)
                     /locus_tag="VGB57_08760"
     CDS             complement(2373..3881)
                     /locus_tag="VGB57_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0131484.1"
                     /translation="MLLHRILAVNVFALAVLAGSIFYLDSFRGRLTTGAVTGAETQVG
                     IIADSLALAEPGEKTALLHRLGARSRTRLRVFGPDGALQMDSWHGAPPTYELRDPANE
                     PWRKNVARLLDNLFDAVAGKPPASPLPLPEPDRIDGWSEAVAASRAPRATNMLRRAPE
                     GTLFISAASRIPASGDVLLLTRNARDIRSIVRDERRTLLIVVLVTLIMSVLLSRFLAR
                     TIALPLRRLARAAHQVRLGRAREVSVPTLPERRDEIGLLARALSDMTQSLRYRIDATE
                     AFAADVTHELKNPLASLRSAVDSLDKIEDPALRRQLLEIVRSDVVRLDRLIVDIAEAS
                     RLDAELSRARFEPVDLGHLLESLLDLWRPRAAERQVRVAFARPRVGSAVVLGDESRLA
                     RAIDNVVDNAISFSPPDGLVEIGAARFGGEVVIAVEDQGPGVPEEARASIFRRFHSVR
                     PEADGFGRHSGLGLAIAKATVDGHDGRIEVESRHDGRSGARFVMHFPEAPSQ"
     gene            complement(3919..4629)
                     /locus_tag="VGB57_08765"
     CDS             complement(3919..4629)
                     /locus_tag="VGB57_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0131485.1"
                     /translation="MPVTIALVDDDRNILTSVSIALQAEGFATRVYSDGEAALKALVD
                     NPPDLAVLDVKMPKMDGMELLRRLRERLDLPVIFLTSKDEEIDEALGLALGADDYVTK
                     PFSQRLLIARIRAILRRAEARAAPADDAQAEAETPMIVRGRLAMDPARHRVSWDGRDV
                     TLTVTEFMILEALAQRPGVVKSRNQLMDVAYQDDIYVDDRTIDSHIKRLRRKFRAADP
                     EFKGIETLYGVGYRFAEE"
BASE COUNT          794 a         1650 c         1663 g          735 t
ORIGIN      
        1 aggatgtcgg cattggtttc gatcacgtcg ccatgcgcgg cctcgacctc ggcgatcagg
       61 gtctcgagca gggggtggga ggggcccgcg gcgaagaaca ggatggcggg gagccggccg
      121 gcctcgcgcg cctcggtcag gatgcgaagc ccctcggcga ggaacatgcc ctgccgccgc
      181 cggcccttct tttcgcgaag ggcgcggacg tccttgacga gggggttgga gaaagcggtg
      241 atctggcgcg gcatcagttc cccggcgaag gccggggccc agacggcgcc ggagctgggc
      301 cccggccttc gccggggaac aaggtgaagc gtcccatcac tcctcgccga agcgatcctc
      361 gaccagggcg accagcttct ccagcgcctc ctcggcgccc tcgccctccg tgtggatggt
      421 gacgcaatcg ccgaatgccg ccccaagcat catcaggccc atgatcgagg tgccgcacac
      481 ccggctgccg tccttctcga cctcgacgcc ggcagggagc ccggcggcca tggtcacgaa
      541 cctggcgctg gcgcgggcgt ggaggccgcg cttgttcctc atctcgaccg tccggctggc
      601 cacgctcaga cggtctcccc gagaatctcc gaggcgaccg agatatattt gcgccccgcc
      661 tcgcgggcgg cggcgaccgc ggccttgacg tcgagcaatt tgcgggcgcc ctcgagccgg
      721 atcagcatcg gcagattgac cccggcgatg acctcgatct tctccgactt catcagcgag
      781 atggcgaggt tggagggcgt gccgccgaac aggtcggtca ggatgatgac cccgcttccg
      841 tcgtcgacct tggcgatcgc ctcggcgatg tcgttgcggc ggccctccat atcgtcctcg
      901 gggccgatgc agatggcctc gatccgctcc tgcggaccca cgacatgctc catcgcccga
      961 acgaactcag ccgcaagccg gccatgggtc accaaaacca gaccaatcat cagacttccc
     1021 aatcctgcgg cttgcccccg ccgccgcctt cgaccccctc gcgcgggagc gaggcgaggt
     1081 cgcgatgctc gaccgtgggc gaaaatcccg cctcgcgcaa ccgggcagcg acgcgctcgg
     1141 cgacgtggac cgagcggtgt cttcccccgg tacagccgaa cgccatcgtc acataggatt
     1201 tcccttccgc gccgtagcgc ggaagcaggg taaggatcag gtcctcgatc ttcgtgagcg
     1261 cctcgccata ggcctcgtcg ccggagatat agtcccccac ggcgggatcg agaccggtga
     1321 gatcgcgaag cgaatccacc caatagggat tcctgaggaa cctcagatcg aacacattgt
     1381 cggcattgcg cggaatgccg cgggcgaagc cgaacgacat gacggacagg gtcggcgcga
     1441 tcgcccccga gctgaagcgc tggcggatct gctgctggag cgcgttgggc gcggtgtcgg
     1501 tggtgtcgac gacgtggtcg gcccagcgcc tcagcggctc catcagctcg cgctccgcgg
     1561 cgatcccgtc ggtggccggg cggtccgggg cgagcgggtg gcgccggcga gtctccgaat
     1621 agcggcgaag gagctccgat ccggcgcagt cgaaatagag cgtttcgaca ggcacccgct
     1681 gctcgctcga aagcgccttg atctgctgga cgattcgctt ggcgtcgaag tcgcgggtgc
     1741 ggctgtcgac tccgatggcg agcggccgcc gccgccgcga attgcccttg ccgagcggag
     1801 tttcgagcaa atcctcgagc agggacaaag gcaaattgtc gacgacttcc cagccgagat
     1861 cctcgagcgt cctcagcgcg gtcgacttgc cggcgccgga caggccggtg acgagcagga
     1921 tacgctgcgg ggccgcggtg gcggtcaaag gggaagtccg aaatgcacga gcgcatattc
     1981 tagcttgagc gcggccgacg gctcaagcgc cgcgagcgcg acgaacggga tatccacacc
     2041 ggcgagcccg atcgtcgcgg gttccggcaa ccgctccggg gtcgagccga gatccgcata
     2101 gaggcagacc ggaacgtccg ccgcaggttc cagcgcgatt atcccaacgc ccctcagctc
     2161 gatcttgccg acgatcgtcg ccggggcccg tgccagaaga gtcccgccga cgcagcgaag
     2221 ctcggtataa tcgtcgctga ccagcgccgc cccgcggtcg atcaggcgca gggcgaggtc
     2281 cgatttgccc gcgcccgacc ggcccgcgag caggattccg cggccgccga tcgccacgca
     2341 ggacgcatgg accgtctcgt cggcaagcag gctcattggg acggcgcctc cgggaaatgc
     2401 atgacgaagc gggcgccgct ccggccgtcg tggcggctct ccacctcgat ccgcccgtca
     2461 tggccgtcga cggtcgcctt ggcgatggcg aggccgaggc cggaatgccg gccgaagccg
     2521 tccgcctcgg gtcggacgct gtggaagcgg cggaagatcg acgccctcgc ttcctccgga
     2581 acgcccggcc cctggtcctc gacggcgatc acgacctcgc cgccgaatcg agccgcgccg
     2641 atctcgacca ggccgtccgg cggggagaag gagatggcgt tgtcgacgac attgtcgatc
     2701 gcccgggcga gcctcgattc gtcgccgagc acgaccgcgc ttccgacccg cggccgggcg
     2761 aaagcgaccc ggacctgccg ctccgccgcc cgcggccgcc acaggtcgag cagggattcg
     2821 agaaggtggc cgaggtcgac cggctcgaag cgggcccggc tcagctcggc gtcgagccgc
     2881 gaggcttcgg cgatgtcgac gatcagcctg tcgaggcgga cgacgtcgct tcgaacgatc
     2941 tcgagaagct ggcggcgaag cgccggatcc tcgatcttgt ccaggctgtc gacggcggag
     3001 cggagcgagg cgagcgggtt cttcagctca tgggtgacgt cggcggcgaa agcctcggtg
     3061 gcgtcgatcc ggtaacgcag cgactgagtc atgtcgctca gcgccctggc gagcaggccg
     3121 atctcgtcgc ggcgctccgg gagggtgggg accgacactt cccgggcgcg gccgaggcgg
     3181 acctggtggg cggcgcgggc aaggcggcga agcggcaggg caatggtcct tgccaggaaa
     3241 cgcgagagca gcaccgacat gatcagggtg accagcacca cgatcagcaa ggtgcgccgc
     3301 tcgtcgcgaa cgatgctcct gatgtcccgc gcgttgcggg tgaggagaag cacgtcgccc
     3361 gaagccggaa tcctggacgc ggcggagatg aacaaggtcc cttccggcgc ccggcgcagc
     3421 atattggtgg cgcggggcgc ccgcgaagcc gcgaccgctt cgctccagcc gtcgatccgg
     3481 tccggctccg gcagtggcaa aggcgaggcc ggcggcttgc ccgcgacggc gtcgaacaga
     3541 ttgtcgagca gccgcgcgac attcttgcgc cacggctcat tcgccggatc gcgcagctcg
     3601 taggtcggcg gcgcgccgtg ccagctgtcc atctgcaacg cgccgtcggg accgaacacg
     3661 cgcagccggg tgcgcgagcg cgcgccgagg cgatgaagca gagccgtctt ctcccccggc
     3721 tcggcgagcg cgagcgaatc ggcgatgatc ccgacctggg tctccgcccc ggtcaccgcg
     3781 cccgtggtca gccgcccgcg gaagctgtcg agatagaaga tgcttccggc gaggacggcg
     3841 agggcgaaga cgttgacggc gaggatgcgg tggaggagca tccagcgtcc cgaccatttg
     3901 aggtcgaggt ccttctcgtc attcctcggc aaaacggtag cccacgccgt agagcgtctc
     3961 gatccccttg aactcgggat ccgccgcccg gaacttgcgc ctcagccgct tgatgtggct
     4021 gtcgatggtg cggtcgtcga catagatgtc gtcctgatag gcgacgtcca tcagctggtt
     4081 gcgcgatttg accactccgg gccgctgtgc gagcgcctcg aggatcatga actcggtcac
     4141 cgtcagggtg acgtcgcgcc cgtcccagga aacccgatgc cgcgccgggt ccatggctaa
     4201 ccgcccccgg acgatcatcg gagtttccgc ctccgcctgc gcatcgtccg ccggcgccgc
     4261 ccgcgcctcc gcgcgacgca gaatcgctcg gattcgagct atcaggagcc tctgactaaa
     4321 cggtttcgtg acataatcgt ccgcgccgag cgccagcccc agcgcctcgt cgatctcctc
     4381 gtccttcgag gtgaggaata tcaccggcag gtcgagccgc tcgcgaaggc ggcggagcag
     4441 ctccattccg tccatcttcg gcatcttgac gtcgagcacg gcgagatcgg gcgggttgtc
     4501 gaccagggcc ttgagcgccg cctcgccgtc cgaatagacg cgggtggcga agccctcggc
     4561 ctggagcgcg atcgagacgg aggtcaggat gttgcggtcg tcgtcgacca gggcgatggt
     4621 gaccggcatt ctttcccttt tgccccagtt gcaggccccc tttagccgct gagcgtcgtc
     4681 attgcgagga gcgtagcgac gaagcaatcc agcgggcgat agcgctgcac tggattgctt
     4741 cgcttcgctc gcaatgacgg acgcgactct acccgaccac cgccccgatc ccccagacga
     4801 tcccgaccat gatcggaacc ccggcgaggt cgagcaggaa gc
//
LOCUS       DASWNU010000448         4943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_510531, whole genome shotgun sequence.
ACCESSION   DASWNU010000448 DASWNU010000000
VERSION     DASWNU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510531"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..320
                     /locus_tag="VGB57_08770"
     tRNA            243..320
                     /locus_tag="VGB57_08770"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Trp,seq:cca)
     gene            707..904
                     /gene="secE"
                     /locus_tag="VGB57_08775"
     CDS             707..904
                     /gene="secE"
                     /locus_tag="VGB57_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY0131486.1"
                     /translation="MARVNPGEFLRQVRAETGKVSWPTRKETVTTGIMVLIMTALLGI
                     FFFGVDSAFAAIVTALLGLIG"
     gene            939..1478
                     /gene="nusG"
                     /locus_tag="VGB57_08780"
     CDS             939..1478
                     /gene="nusG"
                     /locus_tag="VGB57_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY0131487.1"
                     /translation="MSRWYIIHAYSGFENKVRDAIQSEANRLGLATLVEAVEVPTEKV
                     TEVRRGKKVTSDRKFFPGYVLAKLAMNDDVYHLVKNTPKVTGFLGSSGKPQPITDAEA
                     ARILNTKDEAAAAAPKTKINVDYEIGDQVKVLDGPFASFNGVVEELDFDRGRVKVGVS
                     IFGRATPVELEFEQVELVK"
     gene            1669..1890
                     /locus_tag="VGB57_08785"
     CDS             1669..1890
                     /locus_tag="VGB57_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131488.1"
                     /translation="MNMGPETLRANEWFKAVSAIATNLGSALVAAAFGRWFLVGFDLF
                     VFQWLVAGPMVIWSGLHVLTRLEVDDAAG"
     gene            complement(2165..4072)
                     /locus_tag="VGB57_08790"
     CDS             complement(2165..4072)
                     /locus_tag="VGB57_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0131489.1"
                     /translation="MLRSIGVTAILLASAAPALAQRANENPVEAADDAFGTSVGNERV
                     GIYAPQSARGFSPVQAGNVRINGLYFDYQTDLNSRLIAASNVRVGLTAQGYPFPAPTG
                     VADFSLRLPGSQAVASTVVGIGPFGGPRLEIDAQLPVTETLGLAAGVGVNREEQYYGG
                     HKRVLTAAAVARWRPAEKVEIIPFWSMQDTSGDEGQPIILTAGAYLPPRIERRRYFGP
                     EWAENESRGYNYGLLSTVGLGDWTLRGGAFRSVSHLSRNFNVLFQNASRDGEADREVI
                     YEEGRRSASTSGELRLTRQLIDGDRLHLFHLMTRGRMLDRHIGGSQRFSFGRGRIDEP
                     IVAPEPAFSPGRQTTDEVRQVTAGLGYEGRWRDVGELSLGIQRTSYEKAVVRPSGALP
                     VSKSSPWLFNGTLSVIASPRLVFYAGYSKGLEESPVAPEIAVNRGEAPPAIVTEQMDA
                     GLRYAITPSLRLVAGVFDVRKPYFALDPGRVFRELGERRNRGVEVSLAGQITPRLNVV
                     LGAVFLDATVSGDAVDLGLIGRRPIASVGRTVTAAVNWNLPWVKGLSLDLAYEGTSDR
                     IANAANSLVIPARYALALGGRYRFDLSGKPATFRAQWASVNDAYGFNNVSEGFYYNLP
                     RRFQMSLTVDM"
     gene            4205..>4943
                     /locus_tag="VGB57_08795"
     CDS             4205..>4943
                     /locus_tag="VGB57_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEY0131490.1"
                     /translation="MTEHLGYRHHWIFCVAALAVWLALGWPVLQNLGTATPMRGAPVP
                     ALWLVAFALFGAAVLGAMVLKLRPGLQWTLLAAQIAAVAAMAILVPWAMMSVFLIITA
                     WQVAMETGPARALGWVAFQTLAVIAALAQALNPDMCWILGLSLALQLFFAYTAQTARA
                     LARTNRELRAAQAIIANNVRSAERLRISRELHDAWGHELTALGLQLEIASHVTGPGRA
                     NDHVMQAKGLARALLGKVRDVVATLREA"
BASE COUNT          799 a         1657 c         1659 g          828 t
ORIGIN      
        1 tcccggcttt cgccgggatg acggcaggcg ccgtcccgaa aattgaagga ttaatccggt
       61 gcttttcctg ttctactcct gttcctcggc gactcgccag ctcttcccga ctggcgcgcc
      121 cgccggggag ggcgcggtgc cggcggacca ctttccctcc gccggtgccg cccctctctc
      181 gagcccgggc ggccttgcgc cttcgccccc ttctggctaa tgacccagcg cttccggcgc
      241 ctaggagtgt agctcagttt ggttagagca tcggtctcca aaaccgaggg tcgtgggttc
      301 gaatccctcc actcctgcca cgccgaagcg aggcgcgcag cgtagcagcg cgccgctgcc
      361 cgaagggcac gcgaagccgc cggccagcgc gcgaagcgcg actgacgtca cgcgattcac
      421 tcgcgtgacg gctgcggtct ccggggtgta gcccggagcc tctcaccctc tgtcattccg
      481 gcgaaagccg aatccatgaa cacggacgtg cgaaattccg tcccgccgtg ttcatggatc
      541 ccggatcaag tccgggatga cgctctgccg cccccaccgc ccttgccatc gccccttgcc
      601 ctcgctatct cgcgagcctc gcatgcatcg gcaaagctcg gctgaccggc cccccggtcg
      661 agagcggctg cccgaaaggc gctgaaccga aaaggacgaa ggcgaagtgg caagagtcaa
      721 tcccggcgaa ttcctccgcc aggtcagggc cgagaccggc aaggtctcct ggccgacccg
      781 caaggagacg gtgacgacgg gaatcatggt cctgatcatg accgcgctgc tcgggatctt
      841 cttcttcggg gtcgacagcg ccttcgccgc aatcgtcacc gccctgctcg gcctcatcgg
      901 ctagcccgcc ggcccgccag accaaggtaa gaaaatccat gtcccgctgg tacatcatcc
      961 acgcttattc gggcttcgag aacaaggtcc gcgacgccat ccagtccgag gccaaccggc
     1021 tcggcctcgc caccctggtg gaggcggtcg aggtcccgac ggagaaggtg accgaggtcc
     1081 gccgcggcaa gaaggtgacg agcgaccgca agttcttccc cggctacgtg ctcgccaagc
     1141 tggcgatgaa cgacgacgtc taccacctcg tcaagaacac gcccaaggtc accggcttcc
     1201 tcggctcgag cggcaagccg cagccgatca ccgacgccga ggccgcccgg atcctcaaca
     1261 ccaaggacga agccgccgcc gccgcgccca agaccaagat caacgtcgac tatgagatcg
     1321 gcgatcaggt caaggtgctg gacgggccgt tcgccagctt caacggagtc gtcgaggagc
     1381 tcgatttcga ccgcggcagg gtcaaggtcg gagtctccat tttcggccgc gccacccccg
     1441 tcgagctcga gttcgagcag gtcgagctgg tgaaatgatc ctgtcatgct gacttgtggg
     1501 ttgagccgct cgggcgggcg accggctgga ccctgaacca agttcagggt gacgaagtag
     1561 gtacaggagg ccggggcgag agctccggct ttttttggtt cacgcgaagg acggcaagat
     1621 acggatcact caaaaacggc tagaagttga gtcgaattgc ggtaaccgat gaacatgggt
     1681 ccggagacgc taagggcgaa tgaatggttc aaggctgtgt cggccatcgc cacgaatctc
     1741 ggctccgcac tcgtcgcggc cgcgttcggc cggtggttcc tggtcggctt cgacttgttc
     1801 gtcttccagt ggctggtagc cgggcctatg gtgatctggt cgggcctgca cgttctgacc
     1861 cggttggagg tggatgatgc cgctggatga gatattgagg aattccgcac cggcgatcgg
     1921 gggccttctc gttgccgcgg cggctctggc ctgcgcgcga ttcggccgag ggccgcgggc
     1981 tcggtcgcgc gagacggcgg cctcggtcca tcatcccgcc gaatagacgc gcactcaaag
     2041 gacgaagccc gccgcgccct tttcgcctgg gctcacggga cagtcacatc tttgagcctc
     2101 ggccaggctg ccctgacaat gaaaatccat actgccccgc ttagatactg ccctcaccag
     2161 cccctcacat gtccacggtc aggctcatct ggaagcgacg ggggaggttg taatagaagc
     2221 cttcgctgac gttgttgaag ccgtaggcgt cgttcaccga ggcccattgc gcccggaacg
     2281 tcgccggctt gccggacagg tcgaagcggt agcggccgcc gagcgccaga gcgtagcgcg
     2341 ccgggatgac caggctgttc gcggcattgg ctatcctgtc cgacgtgcct tcgtaggcga
     2401 ggtcgagcga caggcccttc acccagggca ggttccagtt gaccgcggcg gtgacggttc
     2461 ggccgaccga agcgatcggc cgccgtccga tcaggcccag atccaccgca tccccgctca
     2521 ccgtcgcgtc gaggaagacg gcgccgagaa cgacgttgag ccgaggcgtg atctggccgg
     2581 cgagcgacac ctcgactccg cggttgcgcc tttcgccgag ctcgcggaag acccggccgg
     2641 gatcgagcgc gaaatagggc ttgcgaacgt cgaacacgcc ggcgaccagc cgaagcgacg
     2701 gggtgatggc gtagcgcaag ccggcgtcca tctgctcggt gacgatggcg ggcggcgctt
     2761 cgccgcgatt gacggcgatc tcgggggcga ccgggctttc ctccagcccc ttgctgtagc
     2821 cggcgtagaa gacgagcctg gggctggcga tgacggagag ggtgccgttg aacagccacg
     2881 ggctggattt tgagacgggc agggcgccgg acggccggac cacggccttt tcataggaag
     2941 tgcgctggat tcccaggctg agttcgccga cgtcccgcca ccggccttcg tagccgaggc
     3001 ccgcggtgac ctggcgcacc tcgtcggtcg tctgccggcc gggcgagaaa gccggctcgg
     3061 gcgcgacgat cggctcgtcg atccggccgc ggccgaagct gaagcgctgg ctgccgccga
     3121 tatggcggtc cagcatccgg ccgcgagtca tcaggtggaa gaggtggagg cggtccccgt
     3181 cgatcaactg ccgggtgagg cgcagctcgc ccgaggtgga agcggatcgg cgcccctcct
     3241 cgtagatgac ctcccggtcc gcctcgccgt cgcgggaggc gttctggaac aggacgttga
     3301 agttgcgcga gaggtgcgag acggagcgga aggcgccgcc gcgaagggtc cagtcgccca
     3361 ggccgacggt ggagagaagg ccgtaattgt aaccccggga ttcgttctcc gcccattcgg
     3421 ggccgaaata gcgccgccgc tcgatccggg gcgggagata ggcgcccgcg gtgaggatga
     3481 tcggctgccc ctcgtcgccg ctggtgtcct gcatgctcca gaaggggatg atctcgacct
     3541 tctccgccgg gcgccagcgc gccacggcgg cggcggtgag cacgcgcttg tggccgccat
     3601 aatattgctc ctcccggttg actccgacgc cggcggccag ccccagcgtc tccgtgactg
     3661 gaagctgggc gtcgatctcc aggcggggcc ccccgaaggg gccgatgccg acgaccgtgc
     3721 tggcgacggc ctgcgatccc ggcaggcgca gcgaaaagtc ggccactccg gtcggagccg
     3781 ggaacggata gccctgcgcg gtgaggccga cccggacgtt gcttgccgcg atcaggcgtg
     3841 agttgaggtc cgtctgataa tcgaaataga ggccgttgat ccggacgttg ccggcctgca
     3901 cggggctgaa gccgcgcgcg ctctgcgggg catagatgcc cacgcgttcg ttgccgacgc
     3961 tggtgccgaa agcgtcgtcg gcggcctcca ccggattttc gttcgcgcgc tgggccaaag
     4021 ccggcgccgc ggaagcgagc agaatggcgg taacgccgat ggaccgtagc attttctacc
     4081 tcccgcactt cggtcctcgt catctgcccc tccgctccga tccggataag tgccggaggt
     4141 cgcacccccg aaccgtgacc ttcggcctat ggtaaggcgg cccgcgctgt ggcagcattt
     4201 cccaatgacg gagcatctcg gataccggca tcactggatc ttctgcgtcg cggcgctggc
     4261 cgtgtggctg gccctgggtt ggccggtcct ccagaatctc gggaccgcaa cgccgatgcg
     4321 gggggctccc gtgccggcgc tttggcttgt cgccttcgcg ctcttcggag cggccgtcct
     4381 cggcgccatg gtcctgaagc tgcgcccggg cctccaatgg acgttgctgg ccgcccagat
     4441 cgccgcggtc gcggcgatgg cgatcctcgt tccatgggcg atgatgtcgg tcttcctcat
     4501 catcaccgcg tggcaggtgg cgatggagac cgggcccgcc agggcgctgg gctgggtcgc
     4561 cttccagacg ctggccgtca tcgccgcgct cgctcaggct ctcaatccag acatgtgctg
     4621 gatcctcggc ctgtccttgg cgcttcagct gttcttcgcc tacaccgccc agacggcgcg
     4681 cgccctcgcc cggacgaacc gggagctcag ggccgcacag gccatcatcg ccaataatgt
     4741 gcggagcgcc gagcgcctgc ggatctcgcg cgagcttcac gacgcctggg gccacgagct
     4801 caccgcgctc gggcttcagc tcgagatcgc gagccacgtg accgggcccg gacgggcgaa
     4861 cgaccatgtg atgcaggcga agggcctcgc ccgagccctt ctcggcaagg tccgcgacgt
     4921 cgtcgccacc ctgcgcgagg ccg
//
LOCUS       DASWNU010000449         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_486174, whole genome shotgun sequence.
ACCESSION   DASWNU010000449 DASWNU010000000
VERSION     DASWNU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486174"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1234)
                     /locus_tag="VGB57_08800"
     CDS             complement(197..1234)
                     /locus_tag="VGB57_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0131491.1"
                     /translation="MKKSYLAAAAALALAACGQQSGGGTQGQQIQIVGSSTVYPFTTA
                     VAEQFQRTHPGMSAIVESTGTGAGMKLFCAGVGDQHPDMTNASRRMKASEFQDCSKNG
                     VRRVIEMPVGIDGLALIEGKNGANMSLTVRDVYAALAANPFGKPNTAKTWKDVNPALP
                     AVAIQVYGPPPTSGTRDSLAELILEKGCESDPAMKALKDSDKDRHKELCTKIREGDGV
                     YVEAGENDNLLVQKVAANPGAIGILGYSFLEENADKVKGIPLGGVAPTAQTISSLTYP
                     GARLLYLYAKGEHGDVIPGMREFLAEYAKAWAAGGYLERRGLIPSPADVQAKGTEAAT
                     KLAPMTADGLK"
BASE COUNT          228 a          496 c          506 g          295 t
ORIGIN      
        1 gacgaagctg acgccgatga cggcggacgg gctgaagtga gaaagaaggc tcccaattcc
       61 tcccccgggt ttccggggga gggggaccgc gcgaagcgcg gtggaggggt tcttcggagt
      121 cagaaggccc ctccaccacc cttcgggtgg tccccctccc ctgcaaatgc aggggaggat
      181 ttaggttcgc tcttgctcac ttcagcccgt cagccgtcat cggcgccagc ttcgtcgcgg
      241 cttccgtgcc cttcgcctgg acatcggcgg gcgaggggat gaggccgcgg cgctcgagat
      301 agccgcccgc ggcccaggcc ttggcatatt cggcgagaaa ctcgcgcatg cccggaatga
      361 cgtccccatg ctcgcccttg gcgtagaggt agagcagccg ggcgccggga taggttaggc
      421 tggagatggt ctgggcggtc ggagcgactc cgccgagcgg gatgcccttc accttgtcgg
      481 cattctcctc gaggaaggaa tagccgagga ttccgatggc gccgggattg gccgcgacct
      541 tctggacgag cagattgtcg ttctcgcccg cctccacgta gacgccgtcg ccctcgcgga
      601 tcttggtgca caattccttg tgccggtcct tgtcgctgtc cttcagggcc ttcatcgccg
      661 ggtcgctctc gcaacccttc tcgaggatca gctcggcgag gctgtcgcgg gtgccggagg
      721 tcggcggcgg accgtagacc tggatcgcca ccgccggcag ggccggattg acgtccttcc
      781 acgtcttggc ggtattgggc ttgccgaacg ggttggccgc gagcgccgcg tagacgtcgc
      841 ggacggtcag gctcatgttg gcgccgttct tcccctcgat cagggcgagg ccgtcgatcc
      901 cgaccggcat ttcgatgacc cgcctgaccc cgttcttcga gcaatcctga aattcggagg
      961 ccttcatccg ccgcgaggcg ttggtcatgt ccggatgctg gtcgccgact ccggcgcaga
     1021 acagcttcat tccggcgccc gtcccggtcg attcgacgat cgcgctcatc cccggatggg
     1081 tgcgctggaa ctgctccgcg accgcggtcg tgaacggata gacggtcgac gagccgacga
     1141 tctggatctg ctggccctgg gttcccccgc cggactgctg accgcaggcg gcgagcgcca
     1201 aggcggccgc ggcggccaaa tagcttttct tcattcgtgc ctccatcgga ccccgattaa
     1261 cgggccccga ggacctccct aggtcggttc ggcgtaagtc ctgtgactct tgcgtgaccg
     1321 tttcatgaca agggcccggt cattccgggc ttgacccgga atccatgatc accgtgggtg
     1381 accagcctta caccgctgtg ttcatggatt ccggccttcg ccggaatgac gatccggaac
     1441 gcgtggcagc gagacgagca cgatcgtccc ttcgccgagc ttgcttcgga tgtcgaggcg
     1501 gccgcgatgg cggcttacga tatgc
//
LOCUS       DASWNU010000450         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_433732, whole genome shotgun sequence.
ACCESSION   DASWNU010000450 DASWNU010000000
VERSION     DASWNU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433732"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="rplM"
                     /locus_tag="VGB57_08805"
     CDS             complement(<1..331)
                     /gene="rplM"
                     /locus_tag="VGB57_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY0131492.1"
                     /translation="MKALMKTTKSAKPAEVEKKWHLIDADGLVVGRLASIIANILRGK
                     HKPSFTPHVDCGDHVVVVNADKVRFTGKKLADKIYYRHTGYAGGIKGVTAGKVLEGRF
                     PERVLEKA"
     gene            555..1376
                     /locus_tag="VGB57_08810"
     CDS             555..1376
                     /locus_tag="VGB57_08810"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0131493.1"
                     /translation="MTDLARPRLAMAALIGIFVAWAPAGAAPATGDPKEIRARAERGD
                     AGGAYELGLYYSEGPEEARDVPEALRWFRKAAEAGNAPAMTELGHHYRLGREVAVDGA
                     EAARWYRRAAKAGHGPAMFSLGLIHTLGQLVKRDDAEALRWFRRGAEAGHASAMNSVG
                     VMYSNGQGVARDHVQALRWYKRAAEAGDAGGMVNLGARHARGEGTTTDFAEAMRWYLR
                     AAEAGNATAMHNIGFMYAEGQSVARDEAEAKRWYRRAADAGHEGSRMLLDGPGLD"
     gene            complement(1379..1708)
                     /gene="cutA"
                     /locus_tag="VGB57_08815"
     CDS             complement(1379..1708)
                     /gene="cutA"
                     /locus_tag="VGB57_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY0131494.1"
                     /translation="MSPIVTVYATFASDEEARRIARTLVEERLAACANILGACLSIYR
                     WQGEVEEAEEVAALFKTRAGRAEALIARLTELHSYDVPAAVVWPIPEASPAYASWVAA
                     ETPDGGE"
     gene            complement(1705..2787)
                     /locus_tag="VGB57_08820"
     CDS             complement(1705..2787)
                     /locus_tag="VGB57_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0131495.1"
                     /translation="MASPAVARPAMPRAVALWLLAVAALVFLMVVVGGITRLTESGLS
                     IVRWEPLSGTLPPIGEEAWAAEFAAYRQSPQYQLVNSGMSLEDFKNIYFWEYVHRLLG
                     RIIGLAFALPLAWFAWKRAVPRGYGWKLVALLALGGLQGAIGWWMVASGLVDRPDVSH
                     IRLAVHLLMALAIFGATLWLALDLLGPGGEHGAGAARMPTAAIWMLSLLFLQFLFGAY
                     VAGLDAGYAYSSWPKMGDQWFPADAPLLEPFLRNFADNPIMVQFVHRWLAFAVAAAAL
                     VLARNAWRAGHPRAAGALAGAVTVQILLGIFTLLSGVELWIAASHQGMAVLLLAAALV
                     AAHRLGAGAAPAALPAAPGGLRGEPA"
     gene            2934..3272
                     /locus_tag="VGB57_08825"
     CDS             2934..3272
                     /locus_tag="VGB57_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEY0131496.1"
                     /translation="MDRVAIALSGLCLVHCLASAIFVAMLASAGGLLLDPIIHEVGLT
                     FAIALGMLALGRGVVDHGFMMPSAVGGLGLGVMAGSLTLHGDAMGTVYTVLGVAILAL
                     GHDLNRRAVI"
     gene            3412..>3818
                     /locus_tag="VGB57_08830"
     CDS             3412..>3818
                     /locus_tag="VGB57_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0131497.1"
                     /translation="MAHHHHHEAHEGASLMQAAQSSMEKSGEQWTQMRSAVFEALAGF
                     DRPASAYDIADKVSQSQGRRVAANSVYRILDLFVGANLARRVESANAYVANDHPDCLH
                     DCIFLICDTCGQTTHIDDDKLASGVRAAAVQAG"
BASE COUNT          626 a         1249 c         1359 g          584 t
ORIGIN      
        1 ccgccttctc gagcacccgc tccgggaagc ggccctcgag caccttgccg gcggtgacac
       61 ccttgattcc gccggcatag ccggtgtggc ggtaatagat cttgtcggcc agcttcttgc
      121 cggtgaacct gaccttgtcg gcgttgacga cgacgacatg gtcgccgcaa tcgacgtgcg
      181 gagtgaagct cggcttatgc ttgccgcgca ggatgttggc gatgatcgag gcgaggcggc
      241 cgacgaccag cccgtccgca tcgatcagat gccatttctt ctccacctcg gccggcttgg
      301 ccgacttggt ggtcttcatc agcgccttca tggcctgaat ctcccttgga gggtgtgacc
      361 cgtctgaaaa tgaaccgcgc cgcgtcccgg gggagcggcg cctgaagcgg gcaaatggca
      421 gcggggaccc tcaaagtcaa ggaaaagctg gggtttggcg tgaggtatct caataccctg
      481 acaggcgccg ggcgtcttgc gcgcgcgcgt gaagcaggta gacttccctt ccgtcccggt
      541 gggggagcaa tgcaatgact gacctggcaa ggccgcggct ggcgatggcc gcacttatcg
      601 gcattttcgt ggcctgggcc ccggccggcg cggccccggc gaccggggac cccaaggaaa
      661 tccgcgcccg tgccgagcgt ggcgatgccg gcggggcgta cgagctcggc ctctactatt
      721 cggagggccc ggaagaggcg cgcgacgtcc cggaagcgtt gcgctggttc cggaaggccg
      781 ccgaggccgg gaatgctccg gcgatgaccg agctcggtca ccattatcgg ctgggccgcg
      841 aagtggcggt cgacggggcg gaggccgcgc gttggtaccg gcgcgcggcc aaggccggcc
      901 atggtccggc gatgttcagc ctcggcctga tccatacgct cggtcagctg gtgaagcgcg
      961 acgatgcaga agcgctgcgc tggttccgcc gcggggccga ggccggccat gcttcggcca
     1021 tgaacagcgt cggcgtaatg tactcgaacg gccagggcgt ggcacgcgac cacgtccagg
     1081 ccctgcgctg gtacaagcgg gcggccgagg cgggggatgc cgggggcatg gtcaatctcg
     1141 gtgcccgtca tgcgaggggc gagggcacga cgaccgactt cgcggaggcg atgcgctggt
     1201 atctgcgggc ggcggaggcg ggcaatgcca ccgcgatgca caatatcggt ttcatgtatg
     1261 ccgagggtca gagcgtggcc cgggacgagg ccgaggcgaa gcgttggtac cggcgggcgg
     1321 ccgacgcggg ccacgaggga tcgaggatgc tgctcgatgg gcccggactc gactgagctt
     1381 attctccgcc gtccggagtc tccgccgcca cccagctcgc ataggcgggc gaggcctccg
     1441 ggattggcca gacaaccgcc gccggcacat cgtagctgtg cagctcggta aggcgggcga
     1501 tcagcgcctc ggcccgtccg gcccgggtct tgaacagggc cgcgacctct tccgcctcct
     1561 cgacctcgcc ttgccagcgg tagatggaga ggcaggcgcc cagaatgttg gcgcaggcgg
     1621 cgagccgctc ctcgaccagg gtgcgggcga tgcgtcgcgc ctcctcgtcg gaggcgaagg
     1681 tggcgtagac ggtgacgata gggctcatgc cggctctcct cgcaggccgc cgggcgcggc
     1741 agggagggcc gccggcgccg ctcccgcgcc caggcggtgg gccgcgacga gcgcggccgc
     1801 cagaagcagc accgccatgc cctggtgcga ggcggcgatc cacagctcga cgcccgagag
     1861 caaggtgaag attccgagca ggatctgcac cgtcaccgcg ccggccaggg cgccggcggc
     1921 gcgcgggtgc cccgcgcgcc acgcgttgcg ggccaggacg agggccgccg ccgcgacggc
     1981 gaaggcgagc cagcggtgga cgaactggac catgatcggg ttgtcggcga agttcctcag
     2041 gaagggctcg agcagaggcg cgtcggccgg gaaccactgg tcgcccatct tcggccagct
     2101 cgaataagcg tagccggcgt cgaggccggc gacataggcg ccgaacagga attgcaggaa
     2161 gagcagcgac agcatccaga tcgccgccgt cggcatccgc gccgccccgg cgccgtgctc
     2221 gccgccgggg ccgagcaggt cgagcgcgag ccacagggtc gctccgaaga tcgccagcgc
     2281 catgagcagg tggacggcga ggcggatatg gctgacgtcg ggcctgtcga ccaggcccga
     2341 ggcgaccatc caccagccga tcgcgccttg cagcccgccc agggcgagaa gggcgacgag
     2401 cttccaccca tagcctcgcg ggacggcgcg cttccaggcg aaccacgcca agggcaaagc
     2461 gaaggcgagg ccgatgatcc gcccgagcag gcgatggaca tattcccaga aatagatgtt
     2521 cttgaagtcc tcgaggctca tgccgctgtt gacgagctga tattgcgggc tctgcctgta
     2581 ggcggcgaac tcggcggccc aggcctcttc gccgatcggc ggcaaggtgc ccgagagcgg
     2641 ctcccaccgt acgatcgaca gcccggattc ggtcagccgg gtgatcccgc cgacgaccac
     2701 catgaggaag acgagggcgg cgacggcgag cagccacagg gcgacggcgc ggggcatggc
     2761 ggggcgggcg acggcgggac tggccatgcc gcgcgatatg gtcgccgaac cgttgaaagc
     2821 caatgcaact tttttcggaa tgttatgttg tatctttacg gctacgatat tataccaagt
     2881 gccgtcagga gaacggcgag tccatgttcg cgaaggtggg aggaaagggg tcgatggacc
     2941 gggtggcgat cgcgctgtcc gggctttgcc tcgtccattg cctggcgagc gcgatcttcg
     3001 tcgccatgct cgcctccgcc ggcgggcttt tgctcgatcc gatcatccac gaagtcggtc
     3061 tgaccttcgc catcgcgctc ggcatgctcg ccctcggccg cggagtcgtc gatcacggct
     3121 tcatgatgcc atccgcggtg ggaggcctcg gcctgggcgt catggcgggc tcgctcaccc
     3181 tgcatgggga cgccatgggg acggtctaca cggtcctcgg agtcgccatc ctcgcgctcg
     3241 gccacgatct caaccgccga gcggtgatct gaccgtttaa gcgcgtcatt gcgaggagcg
     3301 aagcgacgcg gcaacccaag gggcctccgc ggctgcgctg gattgcttcg cttcgctcgc
     3361 aatgacgggc tgtcccttgt tccggctccg caaaagcctt accatgcgcc catggcgcat
     3421 caccatcacc acgaggcgca cgagggcgct tccctcatgc aggccgcgca aagctcgatg
     3481 gagaaatcgg gcgagcagtg gacccagatg cggtcggccg tcttcgaggc cctggccggc
     3541 ttcgatcggc ccgcgtcagc ctacgacatc gccgacaagg tcagccagtc gcagggccgg
     3601 cgggtggcgg cgaacagcgt ctaccggatc ctcgacctct tcgtcggcgc caatctcgcc
     3661 cgtcgggtgg agagcgccaa cgcctatgtc gccaacgacc atccggactg cctgcacgac
     3721 tgcatcttcc tcatttgcga cacctgcggc cagaccacac atatcgacga cgacaagctg
     3781 gcgagcggcg tccgcgctgc ggccgtccag gcgggttt
//
LOCUS       DASWNU010000451         4061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_380664, whole genome shotgun sequence.
ACCESSION   DASWNU010000451 DASWNU010000000
VERSION     DASWNU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380664"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..2294
                     /locus_tag="VGB57_08835"
     CDS             168..2294
                     /locus_tag="VGB57_08835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY0131498.1"
                     /translation="MKNSLAFAASLLALAAAAPALAGDRHDCIDESCTMVSLIDAAQD
                     QAGSAGSASVDAQRFGSWGIDLAGMDRSVKPGDDFFAYVNGTWARTTPIPADRSSYGA
                     FAVLRDLSEARLRQLVESYPAGDPATGGDQAKVAALYRGFMDEEAAERQDAVPLLSRI
                     APIYQARTRDDIARLMGRSIGGFGSTFFGSGVSDDARNPDQYALYIGQSGLGLGDRDL
                     YLDAKFKPQKDRYLAYVTQMLEMAGWPAAKESAAEVVALETRIAEAHWTRAESRNRDK
                     TYNPMTVAQLESQAPGFPWRTYWQAAGLDRTERAVVSQVTAVPKLARIFAETDLQTLK
                     AWQMFHTADDMSPLLSKRFVDAQFEFRSKFLNGQPQQRDRWKRGIAFAENAMGEAIGR
                     DYVALYFTPDAKAKMDRLVTDLRAAMKARLERLQWMGPETKVEALKKLENFGLKIGYP
                     EKWRDYSALQIVPGDLVGNYERAGRFEWDYDRGRVELPVDKAEWSMSPQTVNAYYSSV
                     KNEIVFPAAILQPPFFDPDADPAVNYGAIGGVIAHEITHGFDDQGRKSDWAGVLRDWW
                     KPEDAAKFETQAARLGAQYAAYEFPNLPGMHINPRVAMGENIADLGGVHLGLEAYRNF
                     LGGKPGPVLDGFTPEQRVFMAWAQVWRTLWRDDALRQQIINGPHSPGEIRAFAPLRNA
                     DEWYKAFDVRPGDKHYIPPEQRARIW"
     gene            2392..3084
                     /locus_tag="VGB57_08840"
     CDS             2392..3084
                     /locus_tag="VGB57_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131499.1"
                     /translation="MNSISPSQMILGVAAIFLAWSIADALVRGEWRSRGHRYRRRDQP
                     VDYWFNLLFSIAGLAMLAWLAIDTQEPESSRAWQVAPAFFLGIPAAFWLVRALQTGSF
                     GRGGIDRAEKPRRFWLLVLGLVAVLALAGWTLTRPKAPEQATFYDKVGPVLASVREQL
                     PNRGPGEFYNVRVDSTTRFICGAVRRPDGKMLRFFGTGGRGRPEAIVESDRSPGFGAS
                     YARLCGGEPILP"
     gene            complement(3045..3758)
                     /locus_tag="VGB57_08845"
     CDS             complement(3045..3758)
                     /locus_tag="VGB57_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131500.1"
                     /translation="MIPRVLIDSAPIPGGGGELRLIQRGEDFWIMLGTNALMSTRLRG
                     SEEALATLVCERMTGRPRPALLIGGLGMGFTLRAALGALGRDAQVAVSELVPAIISWA
                     RGPMAHVHGGSLDDPRVRIVENDVGDLIAAARSSYDSILLDVDNGPDALSRDTNDRLY
                     DLDGLRRARSALKPGGILAIWSAAPDPAFSRRLSKAGFAVEEVSVRAHAGRKGAKHMI
                     WLATRGSQGRMGSPPQRRA"
     gene            3850..>4061
                     /locus_tag="VGB57_08850"
     CDS             3850..>4061
                     /locus_tag="VGB57_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY0131501.1"
                     /translation="MSVAFRRESDEEHKEPRFELPIPVGPNLVTANGLAQAQARVEAL
                     EAFVAAERDEARLEESKRELRYWRTRL"
BASE COUNT          664 a         1377 c         1373 g          647 t
ORIGIN      
        1 acccgtcccg gcgcgggtcc ggacttccgg aattggtgcc tggcaccgat ttataagtct
       61 ttgttttcca acccgcgccg ccgggtttgc gctccgatgc gccacgcact atttgaagga
      121 caggcgcctc aggcgcttca atcccgaaga atccggagac tcgcgtcttg aagaacagcc
      181 tcgccttcgc cgcctcgctg ctggcgctcg ccgccgccgc tcccgccctc gccggcgacc
      241 gccacgattg tatcgacgag agctgcacga tggtgtcgct gattgacgcc gcccaggatc
      301 aggccggcag cgccggctcc gcctcggtgg acgcgcagcg tttcggaagc tgggggatcg
      361 acctcgccgg aatggaccgc tcggtgaagc ccggcgacga cttcttcgct tatgtgaacg
      421 gcacctgggc caggaccacg cccatcccgg ccgaccgctc ctcctacggc gccttcgccg
      481 tcctgcgcga cctctccgag gctcggcttc gccagctcgt cgagagctat ccggcgggcg
      541 acccggccac cggcggcgac caggcgaagg tcgcggcgct ctatcgcggc ttcatggacg
      601 aggaagcggc cgagcgtcag gacgcggtcc cgctcctcag ccggatcgcg cccatctatc
      661 aggccaggac gcgcgacgac atcgcccggc tcatgggccg ctcgatcggc ggtttcggaa
      721 gcaccttctt cggctccgga gtcagcgacg acgccaggaa tcccgatcaa tatgcgctct
      781 acataggcca gtcgggcctc gggctgggcg accgcgacct ctatctggac gccaagttca
      841 agccgcagaa ggaccgttac ctcgcttacg tgacccagat gctggagatg gccggctggc
      901 cggcggcgaa ggaatcggcg gccgaggtcg tggcgctcga gacccggatc gcggaggcgc
      961 actggacccg cgccgagagc cgaaaccgcg acaagaccta caatccgatg accgtggcgc
     1021 agctcgagag ccaggcgccc ggcttcccgt ggcggaccta ttggcaggcc gcgggcctcg
     1081 atcgcaccga gcgcgccgtg gtcagccagg tcacggccgt ccccaagctc gccaggattt
     1141 tcgcggagac ggacctccag accttgaagg cctggcagat gttccacacc gcggacgaca
     1201 tgtcgccctt gctctcgaag cggttcgtgg acgcccagtt cgagttccgc tcgaagttcc
     1261 tgaacggcca gccgcagcag cgcgaccgct ggaagcgcgg catcgccttc gccgagaacg
     1321 cgatgggcga ggcgatcggg cgcgattatg tcgccctcta cttcacgccc gacgccaagg
     1381 cgaagatgga ccggctggtg acggaccttc gagccgcgat gaaggcccgg ctcgagcggc
     1441 tccagtggat gggccccgag accaaggtcg aagccttgaa gaagctcgag aatttcgggc
     1501 tgaagatcgg ctatccggag aaatggcgcg attattcggc gctccagatc gtccccggcg
     1561 acctcgtcgg caattacgag cgcgccggcc gcttcgagtg ggactatgac cggggccggg
     1621 tcgagcttcc ggtcgacaag gcggagtgga gcatgagccc gcagacggtg aacgcttatt
     1681 attcgtcggt gaagaacgag atcgtcttcc cggccgcgat cctccagccg cccttcttcg
     1741 atcccgacgc cgatcccgcc gtcaattacg gagcgatcgg cggagtcatc gcccatgaga
     1801 tcacccacgg cttcgacgac cagggccgca agtcggactg ggcgggcgtg ctgcgcgact
     1861 ggtggaagcc cgaggacgcg gcgaagttcg agacgcaggc ggcccggctg ggcgcgcaat
     1921 atgccgccta cgaattcccg aacctgcccg gaatgcacat caatccgcgg gtcgcgatgg
     1981 gcgagaacat agccgatctg ggcggagtcc atctcggtct cgaagcctat cgcaatttcc
     2041 tcggcggcaa gccggggccg gtgctcgacg gcttcacgcc cgagcagcgc gtgttcatgg
     2101 cctgggcgca ggtgtggcgg acgctgtggc gcgacgatgc tctcaggcag cagatcatca
     2161 acggcccgca cagccccggc gagatccgcg ccttcgcgcc gctccgcaac gcggacgaat
     2221 ggtacaaggc gttcgacgtc cggcccggcg acaagcacta catcccgccc gagcaacgcg
     2281 cccggatctg gtgaccggac ttcgagtcgg cggagcggtg ggcccggttt gccatcgacg
     2341 ttgaaatagc caagaaggga acagaaccga cactcttcca ggctgccgct catgaattcg
     2401 atttccccct cccagatgat cctgggggtc gctgccatct tcctggcctg gtctatcgct
     2461 gatgccttgg ttcggggtga atggcggagt cggggacatc ggtatcggcg gcgggaccag
     2521 ccggtcgatt attggttcaa tcttctgttc tccatcgcgg gcctggcgat gctggcctgg
     2581 ctcgccatcg atacgcagga gcccgaatcg tcgcgcgcat ggcaagtcgc gcccgccttc
     2641 ttcttgggga ttccggcggc tttctggctg gtgcgcgcgt tgcagaccgg gtctttcggc
     2701 cggggcggaa tcgaccgggc cgagaagccg cggcggttct ggctgctggt cctcggcctc
     2761 gttgccgtcc ttgccttagc gggctggacc ctcaccaggc cgaaggcgcc ggagcaggcc
     2821 acattctacg acaaggtggg gcccgtgctc gcctcggtgc gagagcaatt gccgaaccgc
     2881 ggtccgggcg agttctacaa tgtgagggtg gactcgacga cgcgcttcat ttgcggagcg
     2941 gtgcggcgcc cggacgggaa gatgcttcga ttcttcggaa ccggcggtcg gggccggccc
     3001 gaagcgatcg tggaaagcga ccgctcgccg ggcttcggcg cttcctatgc ccgcctttgc
     3061 ggcggcgagc ccatcctgcc ctgagagccc cgagtcgcca gccagatcat gtgcttggcg
     3121 cccttgcgcc cggcatgggc acggacgctc acctcctcca ccgcgaagcc ggccttgctc
     3181 agccggcggc tgaaggcagg gtcgggcgcc gccgaccaga tggcgaggat cccccccggc
     3241 ttcagcgccg accgcgcccg gcgcagcccg tccaggtcgt agaggcggtc gttggtgtcg
     3301 cggctgagcg cgtccgggcc gttgtcgacg tcgagcagga tcgagtcgta gctggagcgg
     3361 gccgccgcga tcagatcgcc gacgtcgttc tcgacgatcc gcacccgggg atcgtccagg
     3421 ctgccgccat ggacatgggc catcggcccc cgcgcccagc ttatgatcgc cggcaccagc
     3481 tcggacaccg ccacctgggc gtcgcggccc agggcgccca gggcggcgcg aagggtgaag
     3541 cccatcccga gcccgccgat caggagcgcc ggccgcggcc gtcccgtcat gcgctcgcag
     3601 acgagggtgg cgagagcctc ctccgatccg cgcaggcggg tgctcatcag cgcgttggtg
     3661 ccgagcatga tccagaaatc ctcgccgcgc tggatcagcc ggagctcgcc gccgccgccg
     3721 gggattgggg cgctgtcgat cagaacgcgg gggatcatcg cccgccccct tagaccatgt
     3781 gtggacaagc gcaacgaagg ctccgacacc ggcgttggtc gaacggatca cggaaaaaag
     3841 gatcgagcct tgagcgtcgc gttccgccgc gagagcgatg aagagcataa ggagccgagg
     3901 ttcgagcttc cgatccccgt cggcccgaac ctggtcaccg cgaacggcct cgcgcaggcg
     3961 caggcccggg tcgaagcgct cgaggccttc gtggccgccg aacgggacga agcgaggctg
     4021 gaggagtcga agcgggagct tcgctactgg cgcacccgcc t
//
LOCUS       DASWNU010000452         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_95661, whole genome shotgun sequence.
ACCESSION   DASWNU010000452 DASWNU010000000
VERSION     DASWNU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95661"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1583
                     /locus_tag="VGB57_08855"
     CDS             135..1583
                     /locus_tag="VGB57_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0131502.1"
                     /translation="MKRFRSGTALALLALLSSCGGGGGGGGGNPAPPVAGPTPTPPPP
                     PPTASACSLTARKEWAASVLKEWYLFPETLPANLNPAAYSTVEDYIDALTATARAQRR
                     DRFFTFLTSIQEDNAFFSSGSSAGFGVRFSYDTGQNRVFVAEAYEGAPALAAGIDRGT
                     ELLAIGTSAGNLQLVSALMASGGPQAVSNALGPSTAGTTRVLRISNASGTREVSVSKT
                     DYTLTPVSNRYGAKIIDDGGRRAGYVNLRTFIDTADPALRNAFASFKAAGVTDIIVDV
                     RYNGGGLVSIANLIGDLMGADRSPSDVLSFTTFRPEKSSNNSTRFFQPKPQSIAPTRV
                     AFIGTTGSASASELIINAFIPYLHNRAALVGTNTYGKPVGQIALDQAECDDRLRVIAF
                     KTENAARQGDYYNGLASVVEASCAAGDDIAHQLGDPAETSTRAALDFLAGRVCTPISA
                     SAGGITAQSAGKREPLVPQRPSVVQREAPGTF"
BASE COUNT          283 a          628 c          541 g          284 t
ORIGIN      
        1 ccgattccag ccgggccccg ggcgtgacgg aggcccttgg tagccgccgc cgcgctccgc
       61 gcaaaatata agctgactcg aggctctgac ttcgctaaat agttgaagaa tctgtagagc
      121 ttaggggatt ggggatgaag cgttttcggt ctggaacggc actcgctctg cttgcgctgc
      181 tgtcgtcttg cggcggcggt ggaggcggcg gcggaggcaa ccccgcgccg cccgttgctg
      241 gaccgacgcc cacgcccccg ccgcccccgc cgacggcttc ggcctgctcc ctgaccgccc
      301 ggaaggagtg ggccgcctcg gtgctgaaag aatggtatct gttccccgag accctgcccg
      361 cgaacctcaa ccccgccgct tattccaccg tcgaggatta tatcgatgcc ctgaccgcga
      421 ccgcgcgggc ccagcgccgg gaccgcttct tcaccttcct gacctcgatc caggaggaca
      481 acgccttctt ctcctccgga tccagcgcgg gcttcggagt gcgcttctcc tacgacaccg
      541 gccagaaccg ggtcttcgtc gccgaagctt atgaaggcgc tccggcgctc gcggctggga
      601 tcgaccgggg caccgagctc cttgcgatcg gcaccagcgc cggcaatttg cagctggtca
      661 gcgcgctcat ggcctcgggg ggtccccagg ccgtcagcaa cgcgctcggg ccgtcgaccg
      721 cgggcacgac ccgggtgctt cggatcagca acgcttcggg cactcgcgag gtcagcgtct
      781 ccaagaccga ttacacgctg accccggtat cgaaccgcta cggagcgaag atcatcgacg
      841 atggcggccg cagggccggc tacgtcaatt tgcgcacctt catcgacacc gccgatccgg
      901 cgcttcgcaa cgccttcgcc agcttcaagg cggcgggagt caccgacatc atcgtcgacg
      961 tccgctacaa tggcggcggc ctcgtctcga tcgccaacct gatcggcgac ctgatgggcg
     1021 ccgaccggtc gcccagcgac gtgctcagct tcaccacctt ccggccggag aaatcctcga
     1081 acaactcgac ccgcttcttc cagccgaagc cgcaatccat cgctccgacc cgggtcgcct
     1141 tcatcggcac caccggctcg gcctcggcga gcgagctgat catcaacgcc ttcatcccct
     1201 atctgcacaa tcgggcggcg ctggtcggaa ccaacaccta cggcaagccg gtcggccaga
     1261 tcgctctcga ccaggcggag tgcgacgacc ggcttcgggt gatcgccttc aagaccgaga
     1321 atgcggcgcg tcagggcgat tattataacg gccttgcaag cgtggtggag gcgagctgcg
     1381 ccgccggcga cgacatcgcg caccagctcg gcgacccggc ggagacgtcg acccgagcgg
     1441 ccctggactt cctggcaggc cgggtctgca ctccgatcag cgcgtccgcc ggcgggatca
     1501 ccgcccagtc ggcgggcaag cgcgagccgc tggttccgca gcgcccgagc gtcgtccagc
     1561 gcgaggcgcc cggcaccttc tagaacgtcg ggcttcagat cctcatccct cggcctcgaa
     1621 gcccgaagcg cccggccgga agtaccgaag tttcggcttc ccggagaccc aggcgtcttc
     1681 cgcccctgtt cagggcggag gacatcgtga agggcgcgtt cgctcattcc gccatg
//
LOCUS       DASWNU010000453         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_486286, whole genome shotgun sequence.
ACCESSION   DASWNU010000453 DASWNU010000000
VERSION     DASWNU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486286"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGB57_08860"
     CDS             <1..452
                     /locus_tag="VGB57_08860"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515608.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and pyruvate from
                     2-dehydro-3-deoxy-D-galactonate 6-phosphate; functions in
                     galactonate metabolism; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY0131503.1"
                     /translation="ASLGDRALIGAGTVLDPAQIEQVKDAGGRLIVSPSTDPAVIRAA
                     VAAGLISSPGYFTPSEAFTAIEAGAHALKLFPAEGATPALLKAQKAVLPKEVPVIVVG
                     GVTPDSMRSWRDAGADGFGLGSGLYKPGQSPAETADKARAYIAGLAA"
     gene            452..1381
                     /locus_tag="VGB57_08865"
     CDS             452..1381
                     /locus_tag="VGB57_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0131504.1"
                     /translation="MRPLRIAIIGFGKIAADQHVPSIAANPRFELAGCSSRSGVGPAP
                     QFTDWRQMLDQVEGIEAVAITTPPSPRYEIARECIERGLHTLLEKPPTDTLSEIEDLA
                     CLAEGREVTLFTTWHAQHNPAVAAAAKALAGKRIAAMDILWHEDVRKWHPGQRWIWET
                     GGFGVFDPGINAFSIATKIFPGTLFVERAELSYPEDAQTPIAAEIEFASADADGQLRC
                     SLDWRRTEGEEWTITIRTAEGTEVRLENGGSLLSIDGERQDFESPGEYPDIYARFVEL
                     IDERRSEVDVVPLRLVADCLLVGSRHMVEPAPA"
     gene            1432..>1847
                     /locus_tag="VGB57_08870"
     CDS             1432..>1847
                     /locus_tag="VGB57_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0131505.1"
                     /translation="MDLAVTDELVRTDQAFAGNRLLATFPAASRALIEPYSTVVDLDL
                     GSVVHAAGQDVITTFFPFGTTMISLCRDLSDGRTIEVASIGQEGAVGGIVSCGHAPAF
                     ARAEVIVAGPALRVPMDKLEETKLSSGHNRNLFCRFS"
BASE COUNT          300 a          653 c          604 g          290 t
ORIGIN      
        1 ccgccagcct cggcgaccgc gccctgatcg gcgccggcac ggtcctcgat ccggcgcaaa
       61 ttgaacaagt gaaagacgcc ggcgggcggc tgatcgtctc gccgagcacc gatccggcgg
      121 taatcagagc ggccgtggcg gcgggcctca tctcgagccc gggctatttc actccctccg
      181 aagcgttcac ggcgatcgag gccggcgccc acgcgctcaa gctgttcccc gccgaaggtg
      241 caacgcccgc cctgctgaag gcccagaagg ccgtgttgcc gaaggaggtg ccagtgattg
      301 ttgtcggcgg cgtcaccccg gactccatgc gctcgtggcg cgatgccggc gccgacggct
      361 ttggccttgg ctcgggcctg tacaagccag gccagtcgcc ggccgagacc gcagacaagg
      421 cccgcgccta catcgccggg ctggccgcct gatgcgcccg ctccggatcg ccatcatcgg
      481 cttcggcaag atcgccgccg accagcacgt gccgtcgatc gccgccaatc cgcgcttcga
      541 gctcgccggc tgctcgagcc gctcgggagt cgggccggcg ccgcaattca ccgattggcg
      601 ccagatgctc gaccaggtcg aaggcatcga ggcggtggcg atcacgacgc cgccttcacc
      661 ccgctacgag atcgcccgcg aatgcatcga gcgcggcctg cacacgctgc tcgaaaagcc
      721 gccgaccgac acgctgagcg aaatcgagga cctcgcctgc ctcgccgaag gccgcgaggt
      781 gactctcttc accacctggc acgcgcagca caatccggcg gtcgccgcgg ccgccaaggc
      841 gctcgccggc aagcgcatcg ccgcaatgga catcctgtgg cacgaggatg tccgcaaatg
      901 gcacccgggc cagcgctgga tctgggagac gggcggcttt ggagtcttcg atcccggcat
      961 caacgccttc tcgatcgcca ccaagatctt ccccggcacc ctcttcgtcg agcgcgccga
     1021 gctgtcctat cccgaggacg cgcagacgcc gatcgcggcc gagatcgagt ttgccagcgc
     1081 cgacgccgac gggcagctgc gctgcagcct cgactggcgc cggaccgagg gcgaggaatg
     1141 gaccatcacc atccgcacgg ccgaggggac cgaagttcga ctcgagaacg gcggctcgct
     1201 gttgtcgatc gacggtgagc gccaggactt cgagagcccg ggcgaatatc ccgacatcta
     1261 cgcccggttc gtcgagctga tcgacgagcg ccgaagcgag gtcgacgtcg tgccgctgcg
     1321 cctggtcgcc gattgcctgc tggtcggcag ccgccacatg gtcgaacccg ctcccgcgtg
     1381 ataagttgag ggacgccgcg agagtcgtgc cctcgctggg ggagtgagtt catggacctt
     1441 gcagtgacgg atgagctggt gcggaccgac caggcattcg ccggcaaccg gctgctggcg
     1501 acctttccgg ccgcatcccg agcgctgatc gagccgtatt cgacggtcgt cgatctcgac
     1561 ctcggctccg ttgtccacgc cgccggtcag gatgtcatca ccaccttctt cccgttcggg
     1621 acgacgatga tctctctgtg ccgggatctc agcgacggcc gcacgatcga ggtcgcgtca
     1681 atcggccagg agggagctgt cggcggaatc gtcagctgcg gccacgcccc ggcgttcgcc
     1741 cgcgccgaag ttattgtcgc cggtcctgct ctgcgggtgc cgatggacaa gctcgaggaa
     1801 acgaagctga gctcgggcca caaccgcaac ttgttctgcc gcttttc
//
LOCUS       DASWNU010000454         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_147352, whole genome shotgun sequence.
ACCESSION   DASWNU010000454 DASWNU010000000
VERSION     DASWNU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147352"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2491)
                     /locus_tag="VGB57_08875"
     CDS             complement(<1..2491)
                     /locus_tag="VGB57_08875"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY0131506.1"
                     /translation="MNETHSEEAVKDALARARTYSPFLKLQLERFPDMEEALEAGRVE
                     EAVARAADAGSGAPDLAAALRRERSALAAALAVADLAGAIPLERLMETLSDLADRALG
                     RALAAAFAERTPDDVPLGFAIIALGKHGSRELNYSSDVDLLFLYDPATLPLKPREEAG
                     QGALRIGNRLVELLQKRDSEGYVFRVDLRLRPSPGATPIALPVDAAISYYESSALPWE
                     RAAFVRARQVAGDEKVGRYFLDALHPFVWRRSLDFGAIGEVQAITRRIRDHYSQGQVF
                     GPGFDVKRGRGGIREIEFFAQIHQLIHGGREPGLRAPATLDALAALKVAGRIPADAAD
                     DLADAYRLLRTVEHRLQMVDDRQTHSLPADAAALDNVARLHGLGSGDALLGLLEAPVS
                     RCGELYDGLAGHGEDRVPLNPEALESHLRHSGFDDAVSARLRIEGWRGGKARSLRTAA
                     AGEAFEAMLPVLIEAFGRAPDPMRAMNRFDDVVTKLPSGVNFYRLLEARPGLTDHLGT
                     ILSHAPALAEQLGRRPQLLDGLVDASAFEPAPTVEELVRDFSRSEREGEDYQLVLDRV
                     RRRVNERRFALGVQLIVAHSDPIEAAEGYSRIAEAAINVLARAATAEFEARHGRVNGS
                     ELLVMGLGRLGGEALTHASDLDLVYLFSGTHEAESDGPRPLRATDYFNRLAPRVTAAL
                     SVPTASGPLYDVDTRLRPSGTDGMLAVSLESFALYQSEQAWTWEHMALTRARPVFGSA
                     AARAELQAVIDETLRRPRDPARLVADAVAMRTEMAAHKTARRRFDIKLGPGGLVDLEF
                     AVHTLQLKHQIGLHPHLENALEALAAAGLVSD"
     gene            2531..2752
                     /locus_tag="VGB57_08880"
     CDS             2531..2752
                     /locus_tag="VGB57_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131507.1"
                     /translation="MKPQRSLRVLTIVNTLNPRLEAMVHASSRRFVKVAPPTVHAGVG
                     NALREAFAMDGEVRSLAMFEELLVRLDRA"
     gene            complement(2870..>3418)
                     /locus_tag="VGB57_08885"
     CDS             complement(2870..>3418)
                     /locus_tag="VGB57_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0131508.1"
                     /translation="PHLRAFGRSLSGSRDLADDLVQETLLKAWAARKRFQAGTNMRAW
                     TFIILRNLFLSQMRRARFKGEWDEITASKMLAAPASQDRHVELTDMQRALLHLPQPQR
                     EALILVGAGGFAYEEAAEICGCAVGTIKSRVARGRVALEALLTDGKLPSRREHENEGG
                     RSALQSIMSEVDELSRDRDPGL"
BASE COUNT          536 a         1168 c         1195 g          519 t
ORIGIN      
        1 cgtccgagac caggccggca gccgcgagcg cctcgagcgc gttctcgaga tgggggtgga
       61 gcccgatttg gtgcttgagc tggagcgtgt ggacggcgaa ttcgaggtcg accagcccgc
      121 ccgggccgag cttgatgtcg aaccgcctcc ttgccgtctt gtgcgcggcc atctcggtcc
      181 gcatcgccac cgcgtcggcg accagccggg ccgggtcgcg cggcctgcgc agcgtctcgt
      241 cgatcaccgc ctgaagctcg gcgcgcgccg ccgcggagcc gaagacgggg cgggcccggg
      301 tgagcgccat atgctcccag gtccaggcct gctcgctctg gtagagcgcg aagctttcga
      361 ggctgaccgc gagcatcccg tcggtgccgg aggggcgaag cctcgtgtcg acgtcgtaga
      421 gcggccccga agcggtcgga acgctcaaag cggcggtgac gcgcggcgcc agccggttga
      481 aataatcggt ggcgcgcaac ggccggggcc cgtccgattc cgcttcgtgg gtgccgctga
      541 acagatagac gagatcgagg tcggaggcat gggtcagagc ctcgccgccg agccgcccga
      601 gccccatgac cagaagctcg gagccgttca cccgtccgtg ccgggcctcg aactcggcgg
      661 tggcggcgcg cgccaggacg ttgatcgcgg cttccgcgat ccgcgaatag ccttcggcgg
      721 cttcgatcgg atcggaatgg gcgacgatca gctgcacgcc cagcgcgaac cggcgttcgt
      781 tgacccggcg ccggacccgg tcgaggacga gctgataatc ctccccctcg cgctcggagc
      841 gggagaaatc gcgaaccagc tcctcgaccg tcggcgccgg ctcgaaagcg gaggcgtcga
      901 cgagcccgtc gagcagctgc ggccggcggc cgagctgctc ggccagagcg ggggcgtggc
      961 tgaggatggt tccgagatgg tcggtgaggc cggggcgggc ctcgagcagc cggtagaaat
     1021 tcactccgct gggaagcttg gtgacgacgt cgtcgaaccg gttcatcgcc cgcatcggat
     1081 cgggcgcccg gccgaaagcc tcgatcagca ccggaagcat cgcctcgaag gcttcgccgg
     1141 cggcggcggt gcgcagggat cgggccttgc cgccccgcca gccctcgatc cgaagccggg
     1201 ccgagaccgc gtcgtcgaaa ccggagtggc gcaaatgcga ttccagcgct tccgggttca
     1261 acggaaccct gtcctcgcca tggccggcga ggccgtcata caactctccg cagcggctca
     1321 ccggcgcttc cagcagcccg agcaaggcgt cgcccgagcc gaggccgtga agccgggcga
     1381 cattgtccag cgccgccgcg tcggccggca agctatgggt ctgccgatcg tcgaccatct
     1441 gcagccggtg ctcgacggtt cgaagcagcc ggtaggcgtc ggcgaggtcg tcggcggcgt
     1501 cggcggggat acgaccggcc actttcagcg ccgccaaggc gtcgagcgtc gccggcgccc
     1561 tcagccccgg ctcccgaccg ccgtggatga gctggtggat ctgggcgaag aactcgatct
     1621 cgcggattcc gccgcggccg cgcttgacgt cgaagccggg gccgaaaacc tgcccctgcg
     1681 aataatggtc gcggatccgg cgggtgatcg cctggacctc gccgatcgct ccgaaatcga
     1741 gcgagcgccg ccacacgaag ggatggagcg catcgaggaa atagcggccc accttctcgt
     1801 ccccggcgac ctgtcgggcc cgaacgaacg ccgcccgctc ccagggcaga gcggaggatt
     1861 cgtagtagga aatggcggcg tcgaccggca aggcgatcgg agtcgccccg ggcgagggtc
     1921 ggagccgcag gtcgacccgg aaaacatagc cttccgaatc ccgtttctgg agcaattcga
     1981 cgagccggtt gccgatcctc agcgccccct gccccgcctc ctcgcgcggc ttgagcggca
     2041 gggtcgccgg atcgtagagg aagaggagat cgacgtcgga agaataattg agctcgcggc
     2101 tgccatgctt gccgagcgct atgattgcga agcccagcgg cacgtcgtcc ggagtccgct
     2161 ccgcgaaggc ggccgccagc gcccggccca gcgcccggtc ggccaggtcc gagagagtct
     2221 ccatcagccg ctccagcggg atcgccccgg ccaggtccgc caccgccagg gccgccgcga
     2281 gcgcgctccg ctcgcgcctg agcgcggcgg cgagatccgg ggccccggaa ccggcgtcgg
     2341 ccgctcgggc caccgcttct tcgacccggc ccgcctcaag cgcctcttcc atatcgggaa
     2401 agcgttcgag ctggagcttg aggaatggcg agtaggtccg cgcccgggcg agcgcgtcct
     2461 taaccgcttc ttcactatgt gtttcattca tacaacagcg cagatcagtt ccgttgaggg
     2521 cgcgcaacgg ttgaaacccc agcgttcgct gcgcgttctg accattgtga atacgctcaa
     2581 tccgagatta gaagccatgg ttcatgcttc ctcaaggcgc ttcgtgaagg tcgcgccgcc
     2641 gacggtgcat gccggcgtcg gcaacgccct gcgcgaggcg ttcgccatgg atggcgaagt
     2701 ccgttccctc gcgatgttcg aagagcttct ggtccgtctg gatcgggcgt aaccgcaagg
     2761 cgggattgac cgtcatcccg gcgaaagccg ggacctcgca caattagtct caatgggtct
     2821 cgccgaggcc ccggctttcg ccggggtgac gctacggccc accttcgcct taaagccccg
     2881 gatcccgatc ccggctgagt tcgtccactt cgctcatgat gctctgcagg gcggagcggc
     2941 cgccttcgtt ctcatgctcg cggcgcgacg ggagcttgcc gtcggtgagc agcgcctcga
     3001 gcgcgacccg gccgcgggcg acccggctct tgatcgtccc gaccgcgcag ccgcagatct
     3061 cggccgcctc ctcgtaggcg aagccgccgg cgccgaccag gatcagggcc tcgcgctgcg
     3121 gctgcggcag gtggagcagc gcccgctgca tgtcggtcag ctcgacatgg cggtcctggc
     3181 tcgccggcgc ggcgagcatc ttcgacgcgg tgatctcgtc ccactcgccc ttgaagcgcg
     3241 cccggcgcat ctggctgaga aacaaattgc gcaggatgat gaaggtccag gcgcgcatgt
     3301 tggtgccggc ctggaagcgc ttgcgggccg cccaggcctt gagaagcgtt tcctgaacga
     3361 gatcgtccgc aaggtcgcgg ctgcccgaga gcgatcgtcc gaaggcgcgc agatgggg
//
LOCUS       DASWNU010000455         8679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_43656, whole genome shotgun sequence.
ACCESSION   DASWNU010000455 DASWNU010000000
VERSION     DASWNU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43656"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..301)
                     /locus_tag="VGB57_08890"
     CDS             complement(23..301)
                     /locus_tag="VGB57_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131509.1"
                     /translation="MRKTTALAVSLVFASATIFGSAASAGYGPPRDPACDQRAANNCA
                     SNWQALGWPNYELCVGGQQCLECPPNYGYLCPNPDIYNEYATEPNQPW"
     gene            complement(502..804)
                     /locus_tag="VGB57_08895"
     CDS             complement(502..804)
                     /locus_tag="VGB57_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131510.1"
                     /translation="MLKRLKTAALAASLTLAGGAIFGTAASAQYTYPYPYLSSPCWDQ
                     VMSDCEVKWQAWGLTSYSDCGPMEACQVCPGTDGNNCPTINWVDNNEVSRHDSHGH"
     gene            1155..1814
                     /gene="rpe"
                     /locus_tag="VGB57_08900"
     CDS             1155..1814
                     /gene="rpe"
                     /locus_tag="VGB57_08900"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0131511.1"
                     /translation="MQQPVRIAPSILSADFARLGEEVRAVDAAGADWIHVDVLDGHFV
                     PNLTIGPAVVKALRPHSARPFDVHLMISPVDPFLQAFAEAGADIITVHPEAGPHLHRT
                     LQAIRALGKRAGVALNPATPAKMLDYVLEEVDLVLVMSVNPGFGGQSFIDSQLRKIEA
                     VRKQIDKQGLAVDLEVDGGIDSGTARRAIDAGADVLVAGTATFRGGPQHYAANIESLR
                     R"
     gene            1811..3550
                     /locus_tag="VGB57_08905"
     CDS             1811..3550
                     /locus_tag="VGB57_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEY0131512.1"
                     /translation="MSLLEKIDPPEAGEIEPGKRLIRVGGGHGASLGEQLSSLIHRLS
                     WRTPFHALRLRGRHPLKLLAVPKDPVAGDKAVGEKILRGRFVRRGEEFAVESLDFADP
                     ALPADLSDYLQSFEWLRDLAAAATRERGSKLAEKLVQKWLAAHSETVTEPAWRADLWG
                     RRILFWTAYAPYILSSRDSAYRAVLLNTLSRGARHLDKAAEKARPGLARITAWAGVIA
                     AALVVQGGSARLGRGEAGLLRALAASLHDDGGLVSRSPDEQLMLVELLAQLRAVYYAG
                     DREMPEAVADALSGAVGALLGVTLGDEALSSWQGGNMASKRRLAAAVEGSGVRSRPLR
                     QPRGWGYQRLEAKSTVAVFDAAPPPPTRAFAGGCASTLAFELSEGVNRLIVNCGGPGP
                     AAGALPDDLVRALRSSAAHSTLTLGDRNSTAVHDDGSLGKGVAQVELVRDETAGVVMV
                     EASHDGYVRRFGLVHQRQLTLAADGRELRGQDLLTLQGRRRRAEPIPYAVRFHLHPDV
                     EAATTADGQGALLRVRGRTVWQFRCRGGRLAIEDSLWIDGDARLRETSQLVVGGESPS
                     DGTAIAWIFRRAG"
     gene            3695..5275
                     /gene="purH"
                     /locus_tag="VGB57_08910"
     CDS             3695..5275
                     /gene="purH"
                     /locus_tag="VGB57_08910"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0131513.1"
                     /translation="MKINRALLSVSDKTGLVELAEALAARGVELISTGGTARALREAG
                     LEVKDVSDLTAFPEMMDGRVKTLHPKVHGGLLAVRDDPDHVAAMAEHGIGAIDLVVVN
                     LYPFAQTVARGAGRDEIIENIDIGGPSMVRSAAKNHRFVAICVDPDDYPQLVATLQNN
                     DGTTSLEFRKYLAAKAFAATAAYDSAIAQWFAFADQGQMFPDTLPLTLKRGDELRYGE
                     NPHQKAALYLPAGPHSRGIAQAGQVQGKALSYNNLNDADAALELVSEFRDRGPACVIV
                     KHANPCGVATGASLIEAYEAAFACDTVSAFGGIIAVNRPIDEAAAEAMTQIFTEVVIA
                     PDADAAARAVFARKKNLRLLLTGELPDPARPGLTLKSIAGGYLLQSRDNGRVGREQLK
                     VVTSRQPTERELADCLFAWTVAKHVKSNAIVYAKDGATAGIGAGQMNRLESARIAAWK
                     ACDSAEKAGWPEPKTIGSAVASDAFFPFADGLLAAVEAGATAVIQPGGSIRDDEVIAA
                     ADEAGLAMVFTGMRHFRH"
     gene            complement(5272..6858)
                     /locus_tag="VGB57_08915"
     CDS             complement(5272..6858)
                     /locus_tag="VGB57_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0131514.1"
                     /translation="MSTVPPGAGEDIAALAKGGRTNFFGFLIRLAARLPFLFIAGRLY
                     GPEALGAFAYAVIVMEFAAQLATLGLKRGLAQQLSTTDQPHACVVTDAILAGFIASAF
                     FTTILMVFPQAMFPAGNIHGADWLLPMVIFGIAGADIALAALAYRHDIGATVRARAVV
                     EPWTISIAAGVCVLIFPAAEDGTRSRDGLIIAYVISVTAGMVASLWPLYRTYGLPQNW
                     KPNPAKLLYVARLNIPLAAADAIEWGSRRLDIAILGLFVAPYWVGVYYVGQQVASLPQ
                     KLKTSFDPILAPVITQKLAEGDRKAVAKQVRQVGFWIIAAQAGIALALGLPGDAVMGM
                     VGAAFVGGTGALSFLLAAEVVAATAAVSEAALVYVARHRNLMISALMIFVQAALTLLF
                     IQSVKLLPIRPENVQAYQAASAAAALMLALGMASILKARLLGRLLDAPVQGWRWPLVW
                     AAAAAVIVGQFVNALPKSLYWAELAFGIPAILATFGIVVWWKGFTHDDRVLFRMKKGE
                     TPELPPPPGVTPPGAAPPPL"
     gene            complement(6855..>8679)
                     /gene="polA"
                     /locus_tag="VGB57_08920"
     CDS             complement(6855..>8679)
                     /gene="polA"
                     /locus_tag="VGB57_08920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY0131515.1"
                     /translation="RSLYVTVTDEAELDRWIAEATRNGLVAVDTETDGYDCVTAKLVG
                     ISLATECGKACYIPLEHGGHDLLSERPDQIAAATALAKLKPLLEDPAVLKVGHNFKFD
                     WIVFDRRGIKVAPVDDSLVMSFNLDAGGLNSHSMDDLAKKHLDHDCIAFKDVCGSGQK
                     QIKFNQVPLDRATEYAAEDADVTLRLWTRFKTRLPHERVARIYEMVDRPLVPVVARME
                     REGVKVDREELNKLSQEFQTQITALEERICGEAGCQFTIGSPQQLGEVLFGQMGLKGG
                     RKGKSGTWSTDVNELERLSRDGVPIASLVLEWRQLSKLKSTYTDALQQQINKETGRVH
                     TSYSLSGAQTGRLSSTDPNLQNIPIRTEIGRRIRDAFVAEPGYVMLAADYSQIELRLA
                     AHMADVPQLKAAFAEGADIHSLTAQELFGEVTRDTRASAKTINFAILYGISRWGLAGR
                     LSITADEAQAMIDRYFERFPGIRDYIHSTLEGVREAGFTTTLFGRKTHFPMIRSKTQH
                     ERQGAERAAVNAPIQGTSADIIKRAMVRMCPALEREGLGSVRMLMQVHDELVFEVPEG
                     EVEQASAVIRRVMERAAEPAVTLSVPLGVDIGTGPNWGAAH"
BASE COUNT         1369 a         2978 c         2945 g         1387 t
ORIGIN      
        1 cggggagagg gacatatcgt ccctaccagg gctggttcgg ctccgtggca tattcgttgt
       61 agatgtcggg attggggcag agatagccgt agttgggagg gcattcgagg cactgctggc
      121 ctccgacgca aagctcgtaa ttcggccagc ccaacgcctg ccagttgctg gcgcaattgt
      181 tcgcggcccg ctggtcgcac gccgggtccc tcggaggacc gtatccggcc gacgccgccg
      241 agccgaaaat cgtagcgctc gcgaacacca gcgacaccgc cagcgccgtc gtcttcctca
      301 tgctgagttt cctggacatt cctcattctc ccacggttgc cgtaaccccc gcgcgactcg
      361 ctcgctcgga tcggcccgga gccggaacga taggctcgtg ctcgggttcg aacgacgatc
      421 attgggtccg aaaccgttca atcgacccgc ccgggactcg cgcggggccc ggcgggcccc
      481 gcctaagtcg aaggctcccg ctcagtgccc gtggctgtcg tgcctggaca cctcgttgtt
      541 atccacccag ttgatcgtgg ggcaattatt gccgtcggtg cccgggcaaa cctggcaggc
      601 ctccatcggg ccgcaatccg aatagctcgt caaaccccag gcctgccatt tcacctcgca
      661 atcgctcatg acctgatccc agcatggcga cgacaggtac ggataagggt aggtatattg
      721 ggccgaggcc gccgtaccga atatcgcgcc gccggcgagc gtcagggacg ctgccagcgc
      781 cgccgtcttg agtctcttca acatctcatt ctcccacggt gccggaaccc tcgcccgact
      841 cgctcggaac gatcggccgg cgcggcaacg atacgcctcc gctccgcccc gcacgacgag
      901 cattgggtcc gaaacagtcc caatcgggcg gccccgccct gtcccgcgcc ctgcagggga
      961 gagggagggg cccgcctgcc gaaggcaggt gggagggtga gggcctgagc agcgaggccg
     1021 tgccctcacc ccggcgagct tcgctcgcct gtccctctcc cctacaggga gagggactta
     1081 atgcccctca acaaagcggg gataagtcgc gccctttcct tgccgcgcga ctcgggcagg
     1141 gttaacgttc cggcgtgcag cagcccgtcc gcatcgcccc ctccatcctg tcggccgatt
     1201 tcgcccggct cggcgaggag gtgcgcgccg tcgatgcggc cggcgccgac tggatccatg
     1261 tcgacgtgtt ggacgggcat ttcgtgccca acctcaccat cgggccggcg gtggtgaagg
     1321 cgctccgccc gcacagcgcc aggccgttcg acgtccatct gatgatctcg ccggtcgatc
     1381 ccttcctcca ggcgttcgcg gaggcggggg cggacatcat caccgtccat cccgaggcgg
     1441 ggccgcatct ccaccggacg ctccaggcga tccgggcgct cggcaagcgc gctggagtag
     1501 cgctcaatcc ggcgacgccc gccaagatgc tcgattacgt gctcgaggag gtcgacctgg
     1561 tgctggtgat gagcgtcaat ccgggctttg gcgggcaaag cttcatcgac agccagctgc
     1621 gcaagatcga ggcggtgagg aagcagatcg acaagcaggg cctcgccgtc gatctcgagg
     1681 tggacggcgg catcgattcc ggcaccgcgc gccgcgcgat cgacgccgga gccgacgtcc
     1741 tggtcgccgg gacggcgacg ttccgcggcg gtccgcaaca ttatgcggcg aacatcgaga
     1801 gtcttcggcg atgagcctgc tcgagaagat cgatccgccc gaggccggcg agatcgagcc
     1861 gggcaagagg ctgatccggg tcggcggcgg ccacggcgcc tcgctcggcg agcagctctc
     1921 ctcgcttatc caccgccttt cctggcggac cccgttccac gctttgcgcc tgcgcggccg
     1981 ccatccgctc aaattgctcg ccgtacccaa ggatccggtg gccggggaca aagcggtggg
     2041 cgagaagatc ctccgcggcc gcttcgttcg ccgcggcgag gagttcgccg tcgaaagcct
     2101 ggatttcgcc gatcccgctt tgccagccga cctctccgat tatctgcaga gcttcgaatg
     2161 gcttcgcgat ctcgccgccg ccgcgacccg cgagcgcggc tcgaagctcg ccgagaagct
     2221 cgtccagaaa tggctggccg cgcattcgga gacggtgacc gagcccgcct ggcgcgccga
     2281 cctgtggggc cgccggatcc tcttctggac cgcctacgcg ccctacatcc tttcgagccg
     2341 cgactccgct tatcgcgcag tcctgctcaa cacgctgtcg agaggcgcgc gccatctcga
     2401 caaggcggcg gagaaggcgc gtcccggcct cgcccggatc accgcctggg cgggcgtgat
     2461 cgccgcggcg ctcgtcgtcc agggcggctc cgcccgcctc ggccgcggcg aagcggggtt
     2521 gctcagggcg ctcgccgcct cgctccacga cgatggcggc ctcgtcagcc gctcgcccga
     2581 cgagcagctc atgctggtcg agctgctcgc ccagcttcgt gccgtttatt atgcgggcga
     2641 tcgcgagatg cccgaagccg tggccgacgc tttgtccggc gcggtggggg ctctgctcgg
     2701 agtcacgctg ggcgacgagg cgctgtcgag ctggcagggc gggaacatgg cttcgaagcg
     2761 ccggctcgcg gccgcggtcg agggcagcgg cgtccgctcc cgtccgctcc gccagccgcg
     2821 cggctggggc taccagcggc tcgaagcgaa gagcacggtc gccgtgttcg acgcggcgcc
     2881 gccgccgccg acccgggcct tcgccggcgg ctgcgcctcg accctggcct tcgagctgtc
     2941 ggaaggggtg aaccggctca tcgtcaactg cggtggcccg ggccccgcgg ccggcgccct
     3001 gccggacgat ttggtccggg cgctgaggag cagcgccgcc cattcgaccc tcacgctcgg
     3061 cgaccgcaat tcgaccgcgg tccatgacga cggaagcctc ggcaagggcg tcgcgcaggt
     3121 cgagctggtt cgcgacgaaa cggccggagt ggtcatggtc gaggcgagcc acgacggcta
     3181 cgtccgccgc ttcggactcg tccaccagcg ccagctcacc ctcgccgccg acggacgcga
     3241 gcttcgcggc caggaccttc tcaccctcca gggccgacgt cgccgggccg agccgatccc
     3301 ttacgcggtc cgcttccacc tccatccgga cgtagaggcg gcgaccacgg ccgacggcca
     3361 gggcgccctg ctccgggtcc gcggccgcac cgtctggcag ttccgctgcc gcggcggccg
     3421 gctcgccatc gaggacagcc tctggatcga cggcgatgcc cggcttcgcg agacgtctca
     3481 gctggtcgtc ggcggggaga gcccgtccga cggcaccgcc atcgcctgga tcttccgccg
     3541 cgcgggctag gcatcaccga aggagttttt ccgtcattgc gaggagcgaa gcgacgaagc
     3601 aatccagtcc cggcgtccga gccgctggat tgcttcgctt cgctcgcaat gacggttttt
     3661 gcatgacccc gggagcgcaa aattgcagga ccaagtgaag ataaaccgcg ccctgctgtc
     3721 ggtgtcggac aagaccggcc tcgtcgagct cgccgaagcg ctcgccgccc gcggagtcga
     3781 gctgatctcg acgggcggca ccgcccgggc gctgcgcgag gcggggctgg aggtgaagga
     3841 cgtctccgac ctcaccgcct tccccgagat gatggacggg cgggtcaaga ccctccatcc
     3901 caaggtccat ggcggcctcc tcgccgttcg cgacgatccg gatcatgtcg cggcgatggc
     3961 cgagcacggg atcggcgcga tcgacctcgt cgtcgtcaac ctctacccgt tcgcccagac
     4021 cgtggcccgc ggcgcggggc gcgacgagat tatcgagaat atcgacatag gcggtccctc
     4081 gatggtgcgc tcggccgcca agaaccatcg attcgtggcg atttgtgtcg atcccgacga
     4141 ctatcctcag cttgtggcaa ctttgcagaa caatgacggc acgacgtcat tggagttccg
     4201 caagtatctg gcagccaagg cttttgcggc gacggcggcc tacgattcgg cgatcgccca
     4261 gtggttcgcc ttcgccgatc aggggcaaat gtttcccgac accctccccc tcaccctgaa
     4321 gcggggcgac gagctgcgct acggcgagaa cccccaccag aaggctgcac tctatctgcc
     4381 ggcggggccc cacagccgcg gcatcgctca agccgggcag gtgcagggca aggcgctcag
     4441 ctacaacaat ctcaacgacg ccgacgccgc gctcgagctc gtttcggagt tcagggaccg
     4501 cggcccggcc tgcgtcatcg tcaagcacgc caatccctgc ggcgtcgcga ccggagcgag
     4561 cctcatcgag gcctatgagg ccgccttcgc ctgcgacacc gtctcggcct tcggcgggat
     4621 catcgccgtc aaccggccga tcgacgaagc ggcggccgag gcgatgaccc agatcttcac
     4681 cgaggtggtg atcgcgcccg atgccgacgc ggcggcccgg gcggtcttcg cccgaaagaa
     4741 gaatctccgg ctcctcctca ccggcgagct tccggatccc gcccggccgg gtctgacctt
     4801 gaagtcgatc gccggcggct acctgctcca gtcgcgcgac aacggccggg tcgggcgcga
     4861 acagctcaag gtcgtcacca gtcggcagcc caccgagcgg gagctcgctg actgcctgtt
     4921 cgcctggacg gtggccaagc acgtcaagtc caacgccatc gtctatgcga aggacggcgc
     4981 caccgccggc attggcgcgg gccagatgaa ccggctcgag tcggcgcgga tcgccgcctg
     5041 gaaggcgtgc gactcggcgg agaaagcggg ttggcccgag ccgaagacga ttggctcggc
     5101 ggttgcctcc gacgccttct tccccttcgc cgacggtctg ctggcggcgg tcgaggcggg
     5161 cgcgacggcg gtgatccagc ccggcggctc gatccgcgac gacgaggtga tcgcggccgc
     5221 ggacgaagcc gggctcgcca tggtcttcac cggcatgcgc catttccgcc actagagggg
     5281 tggcggagcg gctccgggcg gagtcacccc gggcggcggc ggaagctcgg gcgtctcacc
     5341 cttcttcatc cggaaaagca cccggtcgtc atgggtgaag cccttccacc agacgacgat
     5401 gccgaaggtt gcgagaatcg ccgggattcc gaacgccagc tccgcccaat aaagcgactt
     5461 gggtaaggcg ttgacgaact ggccgacgat caccgcggcg gcggccgccc agaccagcgg
     5521 ccagcgccag ccctgcaccg gcgcatcgag cagcctgccg agcagcctcg ccttgaggat
     5581 ggaggccatc ccgagcgcca gcatcagcgc cgccgccgcg ctggcggcct ggtaggcctg
     5641 gacgttctcc ggccggatcg gcagcagctt gacgctttgg atgaagagga gggtgagcgc
     5701 cgcctggacg aagatcatca gcgccgagat catcagattg cggtgccggg cgacgtagac
     5761 gagggccgct tccgagaccg ccgccgtggc cgcgacgact tcggcggcga gcaggaagga
     5821 cagcgccccg gtgccgccga cgaacgcggc gccgaccatt cccatcaccg cgtcgcccgg
     5881 caggccgagg gcaagagcga tcccggcctg tgcagcgatg atccagaagc cgacctgccg
     5941 cacctgcttg gccaccgcct tgcggtcccc ttcggccagc ttctgggtga tgacgggagc
     6001 gaggatcgga tcgaagctgg tcttcagctt ctgcggaagc gaggcgacct gctgaccgac
     6061 ataatagacc cccacccaat aaggcgcgac gaacaggccg aggatcgcga tgtcgaggcg
     6121 gcgcgagccc cattcgatgg cgtcggcggc ggcgagcggg atgttgaggc gagcgacgta
     6181 gaggagcttg gccggattgg gcttccagtt ctggggcagg ccgtaggtcc ggtagagcgg
     6241 ccaaagcgat gccaccatcc ccgcggtcac cgagatcacg taggcgatga tcaggccgtc
     6301 gcgggaacgg gtgccgtcct ccgcggcggg gaagatcagg acgcacacgc ccgcggcgat
     6361 gctgatcgtc cacggctcga cgacggctcg ggcccgtacc gtcgcgccta tgtcgtgacg
     6421 ataggccagc gcggccagcg cgatgtcggc gccggcgata ccgaagatca ccatcggaag
     6481 cagccagtcg gccccgtgga tgttgccggc cgggaacatc gcctgcggaa agaccatgag
     6541 aatggtggtg aagaaggcgg aggcgatgaa gccggcgagg atcgcgtcgg tgacgacgca
     6601 ggcgtgcggc tggtcggtgg tcgagagctg ctgggcgagg ccgcgcttga ggccgagcgt
     6661 cgcaagctgc gccgcgaact ccatgacgat gacggcatag gcgaaggcgc cgagcgcttc
     6721 cgggccgtag agccggccgg cgatgaacag gaagggcaac cgagcggcga ggcggatcag
     6781 gaaaccgaag aagttggtcc tcccgccctt ggcgagggcg gcgatatcct ctcccgcccc
     6841 gggcgggacg gtgctcaatg ggcggcgccc caattcggcc cggtgccgat gtcgactccg
     6901 agcgggacgc tgagcgtcac ggcaggctcc gccgcccgct ccatcacccg gcggatgacc
     6961 gcgctcgctt gctcgacctc gccttcgggc acttcgaaga cgagctcgtc gtggacctgc
     7021 atcagcatcc gcaccgatcc cagcccctcg cgctccagcg ccgggcacat gcgaaccatc
     7081 gcccgcttga tgatgtcggc gctggtgccc tggatgggcg cgtttaccgc ggcgcgctcc
     7141 gccccctggc gctcgtgctg ggtcttgctt cggatcatcg ggaaatgcgt cttgcggccg
     7201 aacagggtgg tggtgaagcc tgcctcccgc acgccctcca gcgtcgaatg aatatagtcg
     7261 cggatcccgg ggaagcgctc gaaatagcgg tcgatcatcg cctgggcctc gtcggcggtg
     7321 atgctcagcc ggcccgccag gccccagcgc gagatcccgt agaggatggc gaagttgatc
     7381 gtcttggcgc tggccctggt gtcgcgagtg acctccccga acagctcctg ggcggtcagg
     7441 ctgtggatgt cggccccttc cgcgaaggcg gccttgagct gaggcacgtc ggccatgtgg
     7501 gcggcgagtc gaagctcgat ctggctgtag tcggcggcga gcatgacata gcccggctcg
     7561 gcgacgaagg cgtcgcggat ccggcggccg atctcggttc ggatcgggat gttctgaaga
     7621 ttcgggtcgg tggaggagag ccggccggtc tgggcgcccg acagcgaata gctggtgtgg
     7681 acccgccccg tctccttgtt gatctgctgc tgaagcgcgt ccgtataggt cgacttgagc
     7741 ttggagagct gccgccattc gagcaccagg ctggcgatcg gcactccgtc gcgcgaaagc
     7801 cgctcgagct cgttgacgtc cgtggaccag gtgccggact tgcccttgcg gccgcccttc
     7861 aaccccatct ggccgaacag cacctcgccc agctgctgcg ggctgccgat ggtgaactgg
     7921 caaccggcct cgccgcagat cctttcctcc agggcagtga tctgcgtctg aaattcctgg
     7981 ctgagcttgt tcagctcctc ccggtcgacc ttgacgccct cgcgctccat ccgggccacg
     8041 accggcacca gcggccggtc gaccatctcg tagattcgcg ccacccgctc gtgcggcagg
     8101 cgggtcttga agcgagtcca tagccggagc gtcacgtccg cgtcctcggc ggcatattcc
     8161 gtggcccggt cgagcgggac ctggttgaac ttgatctgct tctggcccga tccgcagacg
     8221 tccttgaagg cgatgcagtc gtggtcgaga tgcttcttcg ccagatcgtc catcgaatgg
     8281 ctgttgagcc cgccggcatc gaggttgaag ctcatcacca ggctgtcgtc gaccggggcg
     8341 accttgatcc cgcggcggtc gaagacgatc cagtcgaact tgaaattgtg gccgaccttg
     8401 aggaccgcgg ggtcttccag aagcggcttc agcttcgcca gcgccgtcgc ggccgcaatc
     8461 tgatccggcc gctccgacag cagatcgtgg ccgccatgct cgagcgggat gtagcaggcc
     8521 ttgccgcatt cggtggcgag gctgatcccg accagcttgg cggtgacgca gtcatagccg
     8581 tcggtctcgg tgtcgacggc gaccaggccg ttgcgggtcg cttcggcgat ccagcggtcg
     8641 agttcagctt cgtcggtcac ggtgacgtag agcgaacgg
//
LOCUS       DASWNU010000456         4305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_69526, whole genome shotgun sequence.
ACCESSION   DASWNU010000456 DASWNU010000000
VERSION     DASWNU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69526"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VGB57_08925"
     CDS             complement(<1..333)
                     /locus_tag="VGB57_08925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0131516.1"
                     /translation="MKTLLPPGWPRPSGFSNGISAKGRMVFTAGIIGWDETGTIVAPD
                     LAGQLRQILLNTLAILAEDGAGPEHIVRMTWYIVDIDEYRLGLAGIGAAYKEVIGRHY
                     PAMAVVEVG"
     gene            complement(330..1484)
                     /locus_tag="VGB57_08930"
     CDS             complement(330..1484)
                     /locus_tag="VGB57_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0131517.1"
                     /translation="MADRAFLDWPFFDESHRELAKRLERWCEAELSDDEPEDLDQACR
                     DLVRKLGDGGWLRHCLPDQAGRLDVRSLALIRETLARHSGLADFAFAMQGLGSGTISL
                     FGSDAQKAAYLPAVARGEKIAAFALTEPASGSDVASIETRAEEDGDGWILNGAKTYIS
                     NGGIADYYILFARTGEAPGAKGISAFIVDRDTHGLDDSERIEVIAPHPLATLRFADAR
                     LPSAALLGERGRGFAQAMATLDVFRTTVGAAALGFARRALDEATRRALERRLGSGTLA
                     DNAITQSKLAEMVLDVETSALLVYRAAWLRDVRGQRNSREAALAKLHATDSAQSVIDK
                     AVQMFGGLGVTKGVPVERLYREIRALRIYEGASEVQKVVIARAHLAEYRN"
     gene            complement(1484..2293)
                     /locus_tag="VGB57_08935"
     CDS             complement(1484..2293)
                     /locus_tag="VGB57_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY0131518.1"
                     /translation="MNPETFAPEHFLWDYEDCVATITLNRPERKNPLTFESYAELRDT
                     FRALVYARGVKAVVVAGAGGNFSSGGDVHEIIGPLTRMDMPALLDFTRMTGDLVKAMR
                     ACPQPIVAALDGICVGAGAIVAMASDLRLATPEAKTAFLFTRVGLAGCDMGACAILPR
                     IIGQGRAAELLYTGRVMTAQEGESWGFHNRLVAREALLGEAHSLARTLAAGPTFAHGI
                     TKTQLNQEWNVSLETAIEMEAQAQAICMATGDFRRAFEAFAAKRTPEFKGD"
     gene            complement(2290..3030)
                     /locus_tag="VGB57_08940"
     CDS             complement(2290..3030)
                     /locus_tag="VGB57_08940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0131519.1"
                     /translation="MSRLKGHHAVITGGGTGIGAAIARSLGAEGAKLTLVGRRRDKLE
                     EVASGSFDALVAPADVTVRVEVDRAFALAREAQGPITILVNNAGAAVGIPFAKVTPEV
                     WRGIMAVNLDGMFHCCQAALPDLLAAPSGRIVTIASMAGLHGFAYASPYIAAKHGAVG
                     LTRALAAEYAQKDLRVNAVCPGFVDTDMTVRSVSNITAKTGRSEDDARAELARLNPSG
                     RLISPEEVAAAVLELVLSERNGEAVEIA"
     gene            complement(3158..>4305)
                     /locus_tag="VGB57_08945"
     CDS             complement(3158..>4305)
                     /locus_tag="VGB57_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127564.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEY0131520.1"
                     /translation="SRALGVAVNDPAPPMFAPYRLRELSLDNRIVVSPMAMYSAEEGT
                     PNDFHLVHYGTRAQGGAGLVYTEMTCVSAEGRITPGCAGLYAAEHVEAWKRIVDFVHS
                     NTPAKFCLQLGHSGPKGSTKVGWEGYDVPLETGNWPLMAASDVPWSERNQTPRPMTRA
                     DMAEVREQFVAATLQGLEAGFDMVELHAAHGYLLSSFITPLTNRRDDEYGGSLDNRLR
                     FPLEVFRAMRAAWPADRPMSVRISANDWMGDQGVTPDEAVLIGRAFAEAGADLIDVSA
                     GQTWADCAPVYGRMFQTPFADQIRNEGRVATMAVGNIYESDHVNSILAAGRADLVALA
                     RPHLVDPMWTLRAAAAADYRGIHVPPQYLGGMSQLARNLAREAELKA"
BASE COUNT          643 a         1410 c         1561 g          691 t
ORIGIN      
        1 ccctacctcg accaccgcca tcgcggggta gtggcgtcct atcacctcct tataggcggc
       61 gccgatcccg gcgaggccca ggcggtattc gtcgatgtcg acgatatacc aggtcatccg
      121 gacgatatgc tccgggcccg cgccgtcctc ggcgaggatg gcgagggtgt tgagaaggat
      181 ctggcggagc tggccggcga ggtcgggcgc gacgatcgtc ccggtttcgt cccagccgat
      241 gatcccggcg gtgaacacca tccggccctt ggctgagatg ccgttggaaa agccgctcgg
      301 ccgaggccag ccggggggaa gcagggtctt caattccgat actccgcgag atgggcgcgg
      361 gcgatcacca ctttctgcac ttcggacgcg ccctcgtaaa tgcgcagcgc cctgatctcg
      421 cggtagagcc gctcgaccgg aacgcccttc gtcactccga ggccgccgaa catctgcacc
      481 gccttgtcga tgacgctctg ggcgctgtcg gtggcgtgga gcttggcgag cgccgcctcg
      541 cggctgttgc gctgccccct gacgtcgcgg agccaggcgg cccggtagac gagcagggcc
      601 gacgtctcga cgtccagcac catctcggcg agcttcgact gggtgatggc gttatcggcc
      661 agcgtgccgc tcccgagccg ccgctccagc gcccgccggg tcgcttcgtc gagcgctcgc
      721 cgggcgaagc cgagggccgc ggcgccgacg gtggtgcgga agacgtcgag ggtcgccatc
      781 gcctgcgcga agccgcggcc gcgctcgccg agcagggcgg cggacgggag ccgggcgtcg
      841 gcgaagcgca gcgtggcgag cgggtggggc gcgatcacct cgatccgttc gctgtcgtcc
      901 agcccatgcg tatcgcggtc gacgatgaag gcggaaatgc ctttcgctcc gggcgcctcg
      961 ccggtgcggg cgaagaggat gtaataatcg gcgatgccgc cgttggagat gtaggtcttg
     1021 gcgccattca gaatccagcc gtcgccgtct tcttccgccc tggtttcgat ggaggcgacg
     1081 tccgagccgg atgccggctc ggtgagagcg aaagcggcga tcttctcgcc gcgggcgacg
     1141 gcgggaaggt aggcggcctt ctgggcgtcc gatccgaaca ggctgatcgt ccccgagccg
     1201 agcccctgca tggcgaaggc gaagtcggcg aggccggaat ggcgggccag ggtctcgcgg
     1261 atgagggcca gcgaacggac gtcgagccgg ccggcttggt cggggaggca atggcgaagc
     1321 cagccgccgt cgccgagctt tcgaacgagg tcgcggcagg cctggtcgag gtcttcgggc
     1381 tcatcgtcgg aaagctcggc ttcgcaccag cgctccagcc gcttcgccag ctcgcggtgg
     1441 ctctcgtcga agaaaggcca gtcgaggaag gcgcggtccg ccatcaatcg cccttgaact
     1501 cgggtgtccg cttggccgcg aaggcttcga aggcgcgcct gaagtctccc gtcgccatgc
     1561 agatggcctg ggcctgggcc tccatctcga tcgccgtctc gaggctgacg ttccattcct
     1621 ggttgagctg ggtcttggtg attccatgcg cgaaggtcgg gcctgcggcg agggtgcggg
     1681 cgagggaatg ggcctcgccg agcagggctt cgcgggccac gagccggttg tggaagcccc
     1741 agctttcgcc ctcctgcgcg gtcatgaccc ggccggtgta gagcaattcc gccgcccgcc
     1801 cctggccgat tatccgcggc aggattgcgc aggcgcccat gtcgcagccg gcgagtccga
     1861 cccgggtgaa aaggaaggcg gtcttggcct ccggcgtggc gaggcgaagg tctgacgcca
     1921 tggccacgat cgccccggcg ccgacgcaga tgccgtcgag cgcggcgacg atcggctgcg
     1981 ggcaggcgcg catcgccttg acgaggtcgc ccgtcatccg ggtgaagtcg agcagggccg
     2041 gcatgtccat ccgagtcagc gggccgatga tctcgtggac gtcgccgccc gagctgaaat
     2101 tgcctccggc gccggcgacc accaccgctt tcaccccacg ggcatagacc agcgcccgga
     2161 aggtatcgcg aagctcggca tagctttcga aggtgagcgg gttcttccgc tccggccggt
     2221 tcagggtgat cgtggcgacg cagtcttcat aatcccacag gaaatgttcg ggcgcgaagg
     2281 tttccgggtt catgcgatct ccactgcctc gccgttgcgc tccgagagga ccagctcgag
     2341 caccgccgct gcaacctctt cgggagagat caggcgcccg gaggggttca gccgcgccag
     2401 ctccgcccgg gcatcgtcct cgctccggcc ggtcttggcg gtgatgttcg agacggaccg
     2461 gacggtcatg tcggtgtcga cgaagcccgg gcagaccgcg ttcacccgca agtccttctg
     2521 cgcatattct gcggccaggg cccgggtgag accgaccgcg ccgtgcttgg cggcgatata
     2581 gggcgaagcg taagcgaaac cgtgcaggcc ggccatcgag gcgatggtga cgatccggcc
     2641 ggaaggcgcg gcgagcaggt cgggcaaagc ggcctggcag caatggaaca tcccgtcgag
     2701 attgaccgcc atgataccgc gccagacttc gggcgtgacc ttggcgaagg gaatccccac
     2761 cgccgctccg gcattgttga cgaggatggt gatcggcccc tgcgcctcac gggcgagggc
     2821 aaaggcgcgg tcgacttcga cgcgcacggt gacgtcggcg ggcgcgacca gcgcgtcgaa
     2881 gctgccggag gcgacctcct ccagcttgtc gcggcggcgg ccgaccaatg tcagcttcgc
     2941 gccctcggcg cccaatgatc gggcaatggc ggcgccgatg ccggtgccgc cgccggtgat
     3001 gacggcgtgg tggcctttca gtcgactcac ctgccctcac cctcccaccg cttcgcggcg
     3061 ggcccctccc tctcccctgc agggagaggg gttgttgcgt ccctctccct gcaggggaga
     3121 gggacaggcg cgaagcgcca gggtgagggc agggcggtca cgccttcagc tccgcctcgc
     3181 gcgccaaatt gcgcgccagc tggctcattc cgccgagata ttgaggcggg acatggattc
     3241 cgcggtagtc cgccgccgcg gcggcgcgca gggtccacat cgggtcgacg aggtggggcc
     3301 gggcgagggc gacgaggtcg gctcggccgg cggcgaggat cgagttgacg tggtcgctct
     3361 cgtagatgtt gccgaccgcc atcgtcgcga cccgtccctc gttgcgaatc tggtcggcga
     3421 agggggtctg gaacatccgc ccgtagacgg gggcgcaatc ggcccaggtc tgccccgccg
     3481 aaacgtcgat caggtcggcg ccggcctcgg cgaaggctcg gccgatcagg accgcctcgt
     3541 cgggggtgac cccctggtcg cccatccagt cgttggcgga gattcgaacc gacatcggcc
     3601 tgtccgccgg ccaggcggcc cgcatcgccc ggaagacttc gagcgggaag cggaggcggt
     3661 tgtccaggct gccgccatat tcgtcgtcgc gcctgttggt caggggggtg atgaagctgg
     3721 agaggagata gccgtgggcg gcgtggagct cgaccatgtc gaaccccgct tccaggccct
     3781 gcagcgtcgc cgcgacgaac tgctcgcgca cttcggccat gtcggcgcgg gtcatcggcc
     3841 ggggtgtctg gttccgctcc gaccagggca cgtccgacgc agccatgagc ggccagttgc
     3901 cggtctcgag cgggacgtca tagccttccc agccgacttt ggtgctgccc ttggggccgg
     3961 aatggccgag ctggaggcag aatttcgcgg gcgtattgga gtggacgaag tcgacgatgc
     4021 gcttccatgc ctcgacatgc tccgcagcgt agaggccggc gcagccgggg gtgatccgtc
     4081 cttcggcgga gacgcaggtc atctccgtgt agacgaggcc ggcgccgccc tgggcccggg
     4141 tgccgtaatg gaccaggtgg aagtcgttcg gagtcccctc ctcggccgaa tacatggcca
     4201 tgggcgagac gacgatccgg ttgtcgagac tgagctcgcg gaggcggtag ggcgcgaaca
     4261 tgggcggcgc gggatcgttg accgccacgc ccagcgccct ggact
//
LOCUS       DASWNU010000457         6985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_172592, whole genome shotgun sequence.
ACCESSION   DASWNU010000457 DASWNU010000000
VERSION     DASWNU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172592"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGB57_08950"
     CDS             <1..300
                     /locus_tag="VGB57_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HEY0131521.1"
                     /translation="EMEQALAGTGIDEPALPLYANVTAAPVEEPDEIRRLLVEQVTGM
                     VRWRESVAAMAEAGVDHFVEFGGKVLGPMVKRIAPDSKVTSVVTMDDIDALVKEI"
     gene            425..1165
                     /gene="fabG"
                     /locus_tag="VGB57_08955"
     CDS             425..1165
                     /gene="fabG"
                     /locus_tag="VGB57_08955"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY0131522.1"
                     /translation="MFDLTGMTALVTGASGGIGSAIAEALAGQGARLALSGSNLEKLE
                     AFRAGLGGDHVALAANLSDSAAVDALVPRAVEALDGKLDILVNNAGVTRDNLAMRMKD
                     EEWSDVIRINLEAAFRLARAALKPMMRARHGRIISITSVVGATGNPGQANYAASKAGL
                     VGMSKALAQEVASRNITVNCIAPGFIRSAMTDALPDAQKEALLGRIPAGKLGEGNDVA
                     AAAVYLASREAGYVTGQTLHVNGGMAML"
     gene            1265..1501
                     /locus_tag="VGB57_08960"
     CDS             1265..1501
                     /locus_tag="VGB57_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0131523.1"
                     /translation="MSETADRVKKIVVEHLGVEAEKVTEEASFIDDLGADSLDIVELV
                     MAFEEEFGVEIPDDAAEKISTVKDAIDYIEAHKA"
     gene            1605..2864
                     /gene="fabF"
                     /locus_tag="VGB57_08965"
     CDS             1605..2864
                     /gene="fabF"
                     /locus_tag="VGB57_08965"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY0131524.1"
                     /translation="MRRVVVTGLGMVSPLGPDVETSWKNILASKSGATRITRFDPTDY
                     VCQIACEVKPPEHEYGFDPAKRVDHKVQRQVDPFIIYGLDAAGQALEDAGLTDMTEEE
                     RYRVGVSIGSGIGGLPGIEKESLVLHEKGPRRVSPHFVHGRLINLISGQVSIKYGLMG
                     PNHAVVTACSTGAHSIGDAARMVALDDADVMVAGGAESALCPLGIAGFAQARALSTQY
                     NDQPEKASRPYDKDRDGFVMGEGAGVVILEEYERAKKRGAKIYAEVIGYGMSGDAYHV
                     TAPHPEGAGGYRAMEVAMKRSGLDLSDIDYINAHGTSTPLGDELELNAVRRLFGNAVG
                     KLSMSSTKSAIGHLLGGAGAVEAIFCILAMRDQIVPPTLNLDNPSEGTAGVDLVPHVA
                     KERHVKAVLNNSFGFGGTNASLVMRAV"
     gene            2872..3837
                     /gene="mltG"
                     /locus_tag="VGB57_08970"
     CDS             2872..3837
                     /gene="mltG"
                     /locus_tag="VGB57_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0131525.1"
                     /translation="MKKLLALVLLVVAGLAVGLWFIQGWSGAGPSRQPTPVVIAPGTS
                     MVAAANQLEKAGVIASAERFLLQAKLFGGGESIKAGEYEIPARASHSQVLSLLQSAKT
                     LQRMVTIPEGLPSVLVHERLMKAQALTGDVPVPEEGSVLPNSYSYQRGEARSAVMKRM
                     QAAMERTLAELWPKRSAATVVKTPAEAVTLASIVEKETGKASERRMIAGVYSNRIRQG
                     MKLDADPTVIYPITKGKPLGRRILRSELNANNGYNTYAMAGLPAGPIANPGKESIAAV
                     LNPAETQALYFVADGTGGHVFANTLAEHNANVSKWFAIRRARGEM"
     gene            complement(4013..4903)
                     /locus_tag="VGB57_08975"
     CDS             complement(4013..4903)
                     /locus_tag="VGB57_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY0131526.1"
                     /translation="MARPRNGASSSSTDRRISLDGPSVRLDPRLNAWRSDIADIALAG
                     RLFAPHYASPVLRACGLIPAPLLREPADGAEAVSELLPGEGFAMLDITAGWAWGYCLA
                     DHRVGYVEAIELADPLEPTHIVIEAQAPIQSGSDPLAATLSHLPMGSRLHGEVRGASL
                     EFEGGFVPLAYLRPVGEHEEDPVAVAQRLLGAPYRPGGRTCHGVDCSGLVQLALQLCG
                     IDAPRDTGEQRGLGEPLPEDAPLKRGDLLFCEDHVGMMVDDRMAIQVSWEARKVAVEP
                     FSCAKAPGSDALIERRRLAL"
     gene            complement(4863..5207)
                     /locus_tag="VGB57_08980"
     CDS             complement(4863..5207)
                     /locus_tag="VGB57_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0131527.1"
                     /translation="MQTLISYVRSGQPDLTNRQMALLLLVYLTPGPHTVRGLAHILGV
                     SKPVITRALNTLGTLGYLRRVRDEADRRNVFVAQTSIGQEFLERFERNIEQQESSNRR
                     GAAKERGILLQH"
     gene            complement(5339..6730)
                     /locus_tag="VGB57_08985"
     CDS             complement(5339..6730)
                     /locus_tag="VGB57_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY0131528.1"
                     /translation="MTDLSTLLRPDKGQPATALQLVDKKGFDSWLKARPARVRQAVMA
                     QGFRGEGYQLAILPGEQAEWSAVLGVANVDALSPWCLAKAAESLPEGTYRVDGRGPGQ
                     AALGWLLAQYRFERYKKPERQTGPRVLLTPEPARTEEIVLTAEAVNLVRDLVNLPAGD
                     LGPAELEAAVREVAADCGAEVAVTSGAALDEGYPMIAAVGRGASADRAPRLVELEWGD
                     RRHPRIAIVGKGVCFDSGGLDIKPASGMRIMKKDMGGGAHALALARLVMKSRLPVRLH
                     LLIPAVENSVSGAAFRPGDVLRSRKGLTVEVDNTDAEGRLILGDALARAAEGEPELIL
                     DFATLTGAARTALGPDLPALFANDEALAGALLAGGESAVDPVWRMPLWAPYEDMLSSD
                     IADIANSGDSPMAGAVTAALFLKKFVPEGLPWAHFDTYAWRGSAKPGRPKGGDAFGLR
                     AAWAALSYRYNHS"
     gene            complement(6733..>6985)
                     /locus_tag="VGB57_08990"
     CDS             complement(6733..>6985)
                     /locus_tag="VGB57_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131529.1"
                     /translation="AGFDEILRATAFKRDEVTTDLLCVEIELGNGESIELNEDVIGFD
                     EWLRRVDALPGVDPDWRGKVTQPPFARNETVLFTRQGQG"
BASE COUNT         1203 a         2333 c         2374 g         1075 t
ORIGIN      
        1 gagatggagc aggcgctggc cggcaccggg atcgacgaac cggccctgcc gctctacgcc
       61 aacgtcaccg cggctccggt ggaggaaccg gacgagatcc gccgcctcct cgtcgagcag
      121 gtgaccggca tggtccgctg gcgcgagagc gtcgccgcca tggccgaggc cggagtcgac
      181 catttcgtcg aattcggagg caaggtgctc ggccccatgg tcaagcgcat cgcgccggat
      241 tcgaaggtga ccagcgtcgt cacgatggac gacatcgacg ccctggtgaa ggaaatctga
      301 ggcgggagcg ggaggggagg ccgtcaccct gaacttgttt cagggtccag ttctccgccc
      361 gcaccgcggc tcgtcgttgg atgctgaaac aagttcagca tgacggccct ggacggagga
      421 aagaatgttc gatctcacag gaatgaccgc cctcgtcacc ggcgcctccg gcgggatcgg
      481 ttcggcgatt gccgaggcgc tggcggggca gggcgcgcgg ctggcgctgt caggatcgaa
      541 cttggagaag ctggaagcct ttcgcgcagg gctcggcggg gatcatgtcg cgctggcggc
      601 caacctctcc gattccgctg cagtggatgc gctcgtgccg cgggcggtcg aggcgcttga
      661 cgggaagctc gatatcctcg tcaacaatgc cggcgtgacc cgcgacaacc tcgccatgcg
      721 gatgaaggac gaggaatggt cggacgtcat ccgaatcaat cttgaagccg ccttccggct
      781 ggcgcgggcg gcgctcaagc cgatgatgcg cgcccgccac ggccggatca tctcgatcac
      841 ctcggtggtc ggagccacgg gcaacccggg ccaggccaat tacgccgcgt caaaggcggg
      901 cttggtcgga atgtcgaagg cgctcgccca ggaagtggcg agccgcaaca tcaccgtgaa
      961 ctgcatcgct cccggcttca tccgctcggc gatgaccgac gccttgcccg acgcccagaa
     1021 ggaggcgctg ctcggccgga tcccggccgg caagctcggc gaggggaacg acgttgcggc
     1081 ggccgccgtt tacctcgcca gccgtgaggc cggctacgtc acgggacaga ccctccacgt
     1141 caacggcggc atggcgatgc tgtgacggta gaggaatgat ccgtttcaga tgccgcccct
     1201 tgccagcccc cgcagggagc ggctagggca tcgcaaaact acgactagga agaagggcac
     1261 acacatgagc gagaccgccg atcgcgtgaa gaaaatcgtc gtcgagcatc tcggcgtcga
     1321 agccgagaag gtcaccgagg aagcgagctt catcgacgat ctcggcgccg acagcctcga
     1381 catcgtcgag ctggtcatgg ccttcgagga agagttcggc gtcgagatcc ccgacgacgc
     1441 ggctgagaag atcagcacgg tcaaggacgc gatcgactat atcgaggccc acaaggcctg
     1501 acggccttgt ccgtcggccc ggccgtgacc gagccgcttg acatgaacgg ctccccgccc
     1561 aaggcggggg gccgttcgtc ttttcgccct ttgaggagct tggcatgcgc cgcgtagtcg
     1621 tcaccggtct cggaatggtg tccccgctcg gacccgacgt agagaccagc tggaagaaca
     1681 tcctggcgtc caaaagcggc gcgacccgca tcacccgctt cgatccgacc gactatgtct
     1741 gccagatcgc ctgcgaggtg aagccgccgg agcacgaata tggcttcgat ccggccaaaa
     1801 gggtcgacca caaggtgcag cgccaggtcg atccgttcat catctacggc ctggacgccg
     1861 ccggccaggc gctcgaggat gccggcctca ccgacatgac cgaggaagag cgttaccgcg
     1921 tcggagtgtc gatcggctcg ggcatcggcg ggcttccggg catcgagaag gaatcgctcg
     1981 tcctccacga gaagggaccg cgccgggtca gcccgcattt cgtccacggc cggctgatca
     2041 acctcatctc cggccaggtc tcgatcaaat acggactgat ggggcccaac catgccgtcg
     2101 tcaccgcctg ctcgaccggc gcccattcga tcggcgacgc cgcccggatg gtcgctctgg
     2161 acgatgctga cgtgatggtc gcgggcggcg ccgagagcgc gctctgcccg ctcgggatcg
     2221 ccggcttcgc ccaggcgcgg gcgctttcga cccaatataa cgaccagccg gaaaaggctt
     2281 cgcgccccta cgacaaggac cgcgacggtt tcgtcatggg cgagggcgcg ggagtcgtca
     2341 tcctcgagga atatgagcgg gcgaagaagc gcggcgccaa gatatacgcg gaggtgatcg
     2401 gctacggcat gtcgggcgac gcctatcatg tcaccgcccc gcacccggaa ggcgccggcg
     2461 gctatcgcgc gatggaagtg gcgatgaaac gctccggcct cgacctttcc gacatcgact
     2521 atatcaacgc tcacggcacc tcgaccccgc tcggcgacga gctcgagctg aacgcggtgc
     2581 gccggctgtt cggcaacgcg gtcggcaagc tgtcgatgag ctcgaccaag tcggcgatcg
     2641 gccatctcct cggcggcgcc ggagcggtcg aggcgatttt ctgcatcctc gccatgcgcg
     2701 accagatcgt cccgcccacc ctcaacctcg acaatccgag cgagggcacg gcgggagtcg
     2761 acctcgtgcc ccacgtcgcc aaggagcgcc acgtcaaggc ggtgctgaac aacagcttcg
     2821 gcttcggcgg gaccaatgca agcctggtga tgcgggccgt ctgacgacgc gatgaagaag
     2881 ctcctcgcgc tcgtcctgct ggtcgtggcg ggtctcgccg tcggcctctg gttcatccag
     2941 ggctggagcg gggcggggcc gtcgcgccag ccgacgccgg tggtgatcgc gccgggcacg
     3001 agcatggtgg cggcggccaa ccagctcgag aaggccgggg tgatcgcttc ggccgagcgc
     3061 ttcctgctcc aggccaaatt gttcggcggc ggcgagtcga tcaaggcggg cgaatatgag
     3121 atccccgccc gcgccagcca ctcgcaggtc ctgtccctcc tgcagagcgc gaagaccctc
     3181 cagcggatgg tcacgatccc cgaaggcctg ccgtccgtgc tcgtccacga gcggctcatg
     3241 aaggcgcagg cgctgaccgg cgatgtgccg gtgcccgagg agggcagcgt ccttcccaac
     3301 agctacagct accagcgcgg cgaggcgcgc tcggcggtga tgaagcggat gcaggcggcg
     3361 atggagcgga ccctggccga gctttggccg aagcgaagcg cggcgaccgt ggtcaagacc
     3421 ccggccgagg cggtcaccct cgcctcgatc gtcgagaagg agaccggcaa ggcttcggag
     3481 cgccggatga tcgcgggcgt ctattcgaac cggatccggc aggggatgaa gctcgatgcc
     3541 gatccgaccg tcatctaccc gatcacgaaa ggcaagccgc tcggccgccg catccttcgt
     3601 tccgagctta acgcgaataa cggctacaac acctatgcca tggccggcct tccggcggga
     3661 ccgatcgcca atcccggcaa ggaatcgatc gccgcggtcc tgaacccggc cgagacacag
     3721 gcgctctact tcgtcgccga cggtaccggc ggccacgtct tcgccaacac cctcgccgag
     3781 cacaatgcca acgtcagcaa atggttcgcg atcaggcggg cgagggggga gatgtgatct
     3841 tggcctccct gccgcgaagc ggtggggagg gggaccacac gaagggtggt ggaggggtaa
     3901 ctggcgcagg gttgtgcggt tctcgcggaa tccgcccagg tcagcgcctt ctacccctcc
     3961 accacccttc gggtggtcgc ccctccccat ggccccgcca tggggaggca agtcaaagcg
     4021 ccagcctgcg acgttcgatc agcgcgtcgc tccccggcgc cttcgcgcag ctgaagggct
     4081 ctaccgccac cttccgcgct tcccagctga cctggatcgc catccggtcg tcgaccatca
     4141 ttccgacatg gtcctcgcag aagaggaggt cgccgcgctt cagcggcgcg tcctcgggca
     4201 gcggctcgcc cagcccgcgt tgctcgcctg tgtcgcgggg cgcgtcgatg ccacagagct
     4261 gaagcgcgag ctggacgagg cccgagcaat ccacgccgtg gcaggtgcgt ccgccggggc
     4321 ggtagggggc tccgagcagg cgctgggcga cggccaccgg atcctcctca tgctcgccga
     4381 ccgggcgcag ataggcgagg ggcacgaagc cgccttcgaa ctcgagcgag gcgccacgaa
     4441 cctcgccgtg caggcgcgag cccatcggca gatgggagag ggtcgccgcg agcgggtccg
     4501 atcccgactg gatcggcgcc tgcgcctcga tcacgatatg ggtgggctcg agcgggtcgg
     4561 cgagctcgat cgcctcgaca tagccgaccc ggtgatcggc caggcaatag ccccaggccc
     4621 agccggcggt gatgtcgagc atcgcgaagc cttcgccagg aagaagctcc gagaccgctt
     4681 cggcgccgtc ggctggctcc cgaagcaggg gagccggaat cagcccgcag gcgcgaagca
     4741 ctggggacgc gtaatgcggc gcgaagagtc ggccggcgag cgcgatgtcg gcgatgtcgc
     4801 ttcgccaggc gttgagtcgc ggatcgagcc ggaccgacgg tccgtcgagc gagatcctcc
     4861 ggtcagtgct ggaggaggat gccccgttcc ttggccgcgc cacggcggtt cgagctttcc
     4921 tgctgctcga tattgcgttc gaagcgctca aggaactcct gccctatgct ggtctgggcg
     4981 acgaagacgt tgcggcggtc cgcctcgtcg cgcacccggc gcagatagcc gagcgtgccc
     5041 agcgtattca aggcgcgcgt gatcaccggc ttggacacgc ccaatatatg ggcgaggccg
     5101 cggaccgtat ggggccccgg ggtcaggtag accagcagaa gcagggccat ctggcgattg
     5161 gtgaggtcgg gctggcctga ccgcacgtag ctgatcagag tctgcatcca gctcctgaga
     5221 gcgcgctctg acactggctt gtctccaaat ttccaggcgg cttgttccgg gtcctaaacc
     5281 agtcagcacg gttttcgttt cgttaaatat tgagaattcg acgtaaaagc cgcgcagcct
     5341 aactatggtt ataacgatag gagagtgcag cccaggcggc gcgcagcccg aaagcgtcgc
     5401 cgcccttcgg ccttccgggc ttcgccgagc cccgccaggc ataagtgtcg aaatgggccc
     5461 agggcaggcc ttcgggcacg aatttcttta ggaacagcgc cgcggtgacg gccccggcca
     5521 tgggtgagtc gcccgaattg gcgatgtcgg cgatgtccga ggagagcatg tcttcgtagg
     5581 gcgcccacag cggcatccgc cagaccgggt ccaccgccga ctcgccgccg gcgagcagcg
     5641 caccggccag cgcctcgtca ttggcgaaca gcgccggaag gtccggaccg agcgccgtcc
     5701 gcgccgcccc ggtcagtgtg gcgaagtcga ggatcagctc gggctcgcct tccgccgccc
     5761 tggcgagggc gtcgccgaga atgaggcgcc cttcggcgtc ggtattgtcc acttcgacgg
     5821 tcaggccctt gcggctcctc agcacgtcgc cgggacggaa ggcggcgccg gagacgctgt
     5881 tctcgaccgc cgggatgagg agatggagcc gaaccgggag gcggctcttc atcaccagcc
     5941 gcgccagggc cagagcgtgg gcgccgccgc ccatgtcctt cttcatgatc cgcatgccgg
     6001 aggccggctt gatgtcgagc ccgcccgaat cgaagcagac tcccttgccg acgatggcga
     6061 tccgcggatg gcggcggtcg ccccattcca gctcgacgag gcgcggcgcc ctgtccgccg
     6121 aagcgccgcg gccgacggcg gcgatcatcg gatagccttc gtcgagagcg gcgcccgaag
     6181 tgacggcgac ctcggcgccg cagtccgccg caacctcccg aaccgccgcc tcgagctcgg
     6241 cgggtccgag gtcgccggcc ggaaggttga cgaggtcgcg cacgagattg acggcctcgg
     6301 cggtgaggac gatctcttcg gtccgcgccg gctccggggt gagcagcact cgtgggccgg
     6361 tctgccgctc cggtttcttg tagcgctcga acctgtactg ggcgagcagc cagcccaaag
     6421 cggcctggcc gggcccgcgg ccgtcgaccc ggtatgtgcc ctcgggcagg ctctccgccg
     6481 ccttggccag gcaccagggg ctgagcgcgt cgacattggc gaccccgagc acggccgacc
     6541 attcggcctg ctccccgggc aggatggcga gctgatagcc ctcgccccgg aaaccctgcg
     6601 ccatgaccgc ctgccgaacc cgcgccggcc gagccttcag ccaggagtcg aagcccttct
     6661 tgtcgacgag ctggagcgcg gtcgcgggct ggcccttgtc aggccggagg agagtggaga
     6721 ggtcagtcat cttcaaccct ggccttgacg tgtgaacagc actgtttcgt tgcgggcaaa
     6781 gggcggctgg gtcaccttgc ctcgccaatc ggggtcgacg ccggggagcg catcgacgcg
     6841 ccgcagccac tcgtcaaagc caatgacatc ctcattcagc tcgatacttt caccgttgcc
     6901 caattcgatt tccacgcaaa gcagatcagt cgtcacttca tcccgcttga aggccgtcgc
     6961 gcgcaggatt tcgtcgaaac cggcg
//
LOCUS       DASWNU010000458         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_458534, whole genome shotgun sequence.
ACCESSION   DASWNU010000458 DASWNU010000000
VERSION     DASWNU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458534"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VGB57_08995"
     CDS             complement(<1..1256)
                     /locus_tag="VGB57_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131530.1"
                     /translation="MKQATFQTLTKRSWLGIAAVASVSLLSGAAAVQGEPRTSIHPYL
                     EVQQVATMDFDRGEVLTYTGVGGGIDASVATRRVQATISANYQHRVSWDDKMSDNDVV
                     SGLAAVHVDAVPGVLALDAGAMAARTHADIRLPVPAARTIDSANVAEIYSAYAGPTLT
                     THAGPVAIGASYRLGYVKVDDHSLAGTGLPPGAPRVDRYSSSTVHNATISFGMEPGRL
                     PFGWTVGGGYVREDMNRLDSNFEAKYVRGDVVVPVSPTLAVTGGVGYETMQGSQQDFL
                     RGPGGLPALTPGGNLIPDPSKPRLETIDQDGVMWDAGVIWRPSPRTELQARGGWRYGG
                     ESFTASLSHKINSRSALNASIYDSVSSFGRLLIADLNGLPTKFKAPSNGSLTGNGCVF
                     GNDPGTGRCFGDAFQSIANFNFRNRGA"
     gene            complement(1253..1972)
                     /locus_tag="VGB57_09000"
     CDS             complement(1253..1972)
                     /locus_tag="VGB57_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEY0131531.1"
                     /translation="MNRKSLGTRGFIVPDSPAGPLAEEFRIVKRQLLLGMGAGSPVSA
                     DKRRTILVCSAQPDEGKTFCAVNLALSLAGERDMEVLLVDADFPKPEVLSILGLEAQG
                     AGFVDALADPSLDPERLVIRTDIGGLSVMPAGRYENDVPELLGSDRTARVLARLTEGR
                     PKRIVIFDSPPALMASAAAALAAHVGQLLLVVRADQTNEADLKEAAGLLSGCPQISLL
                     LNGTGFAATGRKFGSYYGYGQ"
     gene            complement(2239..>2974)
                     /locus_tag="VGB57_09005"
     CDS             complement(2239..>2974)
                     /locus_tag="VGB57_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY0131532.1"
                     /translation="AALEAQLAQSAAQGWTDSHPDVVRLRNQIARLKPLAAREPTGAG
                     TMPNPSYVSLRAMVAEKEGQVNAAATRKAQLQSDLAQLGTRQASEPGVAAEQERLNRD
                     YDVLKKQYDKLLEDRDQVRLRTDVRSQTDAVKFKVIDPPSQPSVPLTPNRPLLLTLIL
                     FVGLAAGAGAAYVLGQIQTTFPTQTRLAEATGLPVLGSIGEVRTETAKAKHRQRLIWL
                     GGGAGALGAAWAVLLAVEFWQRSTVA"
BASE COUNT          427 a          974 c         1083 g          490 t
ORIGIN      
        1 gcgccgcggt tgcggaagtt gaagttggcg atcgactgga aggcgtcccc gaagcagcgg
       61 ccggtgccgg ggtcgtttcc gaagacgcat ccgttacccg tcaggctgcc attagacgga
      121 gctttaaatt tcgtcggcaa accattgaga tcggctatca aaagtctccc gaagctgctc
      181 acgctgtcat agatgctggc gttgagcgcc gagcggctgt tgatcttgtg cgagagcgag
      241 gcggtgaagc tctcgccgcc gtagcgccag ccgccccggg cctggagctc ggtgcgcggg
      301 ctcgggcgcc agatgacccc cgcgtcccac atcactccgt cctggtcgat cgtctcgaga
      361 cgcggcttcg agggatcggg gatgagattg ccgccgggcg tgagcgccgg caggccgccc
      421 ggcccgcgca ggaaatcctg ctgcgagccc tgcatcgtct cgtagccgac tccgccggtg
      481 acggcgaggg tcggtgaaac gggcacgacg acgtcgccgc ggacatattt ggcctcgaag
      541 ttggaatcga gccggttcat gtcctcgcgc acgtagccgc cgccgacggt ccagccgaag
      601 ggcagccggc cgggctccat cccgaagctg atcgtcgcat tgtggacggt gctgctcgaa
      661 tagcggtcga cccgcggagc gcccggcgga aggccggtgc cggcgaggct gtggtcgtcg
      721 accttgacgt agccgaggcg gtagctggcg ccgatcgcga ccggcccggc atgggtggtg
      781 agggtcgggc cggcataggc gctgtagatt tcggcgacgt tggcgctgtc gatggtccgc
      841 gccgccggca ccggcagcct gatgtcggca tgggtgcgcg ccgccatggc gccggcatcg
      901 agggcgagca ctccgggaac ggcgtcgaca tggaccgcgg cgaggccgct gaccacgtca
      961 ttgtcgctca tcttgtcgtc ccagctcacc cggtgctggt aattggcgct gatcgtggcc
     1021 tggacccgcc gggtcgcgac ggaggcgtcg atgccgccgc cgacgcccgt gtaggtgagc
     1081 acctcgccac ggtcgaaatc catggtcgcg acctgctgga cttccagata gggatggatg
     1141 ctggttcggg gctcgccctg gaccgcggcc gctccggaga gcaggctgac gctcgcgacg
     1201 gctgcgatgc cgagccaact acgctttgta agcgtttgga aagtagcctg tttcattggc
     1261 cgtatccata ataggagccg aatttccggc ccgtggcggc gaagcccgtg ccgttgagca
     1321 aaaggctgat ctgggggcag cccgagagca ggccggcggc ctccttcagg tccgcttcgt
     1381 tggtctggtc ggccctcacg accagcagca gctggccgac atgggccgcc agcgcggcgg
     1441 ccgcgctggc catcaaggcc ggcggggaat cgaagatcac gatgcgcttg ggccggcctt
     1501 cggtcagtcg cgccagcacc cgggcggtcc ggtccgagcc gagcagctcg ggaacgtcgt
     1561 tctcgtagcg gccggccggc atgaccgaga ggccgccgat gtcggtgcgg atgacgagcc
     1621 gctcgggatc gagcgacgga tcggccagag cgtcgacgaa gccggcgccc tgcgcctcga
     1681 ggccgaggat cgagagcact tccggcttgg ggaagtcggc gtcgacgaga agcacctcca
     1741 tgtcgcgctc gccggccagc gacaaggcca ggttgacggc gcagaaggtc ttgccctcgt
     1801 cgggctgggc cgagcagacg aggatcgtcc ggcgcttgtc ggcgctgacc ggactgcccg
     1861 cgcccattcc gagcaggagc tggcgcttga cgatccggaa ctcctcggcg agcgggcccg
     1921 ccgggctgtc gggcacgatg aagccccgtg tcccgagcga cttgcggttc acctcggctt
     1981 tcgtttcggt taccgcgggc aggaaagtcc ggcttgcctc tgaagctccc cggcgaaggc
     2041 ccgggtccag ctcttccgcg gcacttggcc ccggccttcg ccggggagca gcctcgggtt
     2101 cgagcggtgg ctcttcgacg cggggcgagg gcgcagcttg ggccgtcgtc ggcgaaccga
     2161 agccgtaaag ctccgccgcc cgctccagaa gcgagcccga gtggctgagg gggctgtgct
     2221 tgttcatctc gctctccctc acgcgaccgt gctgcgctgc cagaattcga ccgcgagcag
     2281 cacggcccag gccgctccga gcgctcccgc gccgccgccc agccagatca gccgctgccg
     2341 gtgcttcgcc ttcgccgttt cggtacgcac ctcgccgatc gagccgagca ccggcagccc
     2401 ggtcgcttcg gcgagccggg tctgggtcgg gaaggtggtc tggatctgcc ccagcacgta
     2461 ggccgcgccc gcgccggcgg cgaggccgac gaacaggatc agggtgagca gcagcggccg
     2521 gttgggagtc aacggaacgc tcggctggct ggggggatcg atgaccttga acttgaccgc
     2581 gtcggtctgg ctccggacat cggtcctgag gcgcacctgg tcgcggtcct cgagcaattt
     2641 gtcatattgc ttcttcagca cgtcgtaatc gcggttgagc cgctcctgct ccgcggcgac
     2701 tcccggctcc gaggcctggc gggtgccgag ctgggcgagg tcggactgga gctgcgcctt
     2761 gcgggtcgcg gcggcgttca cctggccttc cttctcggcg accatcgcgc gcagggatac
     2821 gtaggagggg ttgggcatgg tcccggcgcc ggtcggctcg cgcgccgcga gcggcttgag
     2881 cctggcgatc tggttgcgaa gcctgaccac gtcgggatga gaatcggtcc agccctgggc
     2941 tgcggactgg gcgagctggg cctcgagcgc cgcg
//
LOCUS       DASWNU010000459         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_458567, whole genome shotgun sequence.
ACCESSION   DASWNU010000459 DASWNU010000000
VERSION     DASWNU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458567"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1687
                     /locus_tag="VGB57_09010"
     CDS             353..1687
                     /locus_tag="VGB57_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0131533.1"
                     /translation="MSAEFTQVMVAERIGLFDSDGSLEANCRELAALLANDSVRVARR
                     FWSHLAGAPEVNAAFSEAEMDENVRKILPYMAAKYTDVRGQAWADMVGGYVLDAQSTH
                     VSLTTLLSSIGAAAEEGHDILVDKLSGDPERLGRLARCLTKATLLEADMFAAHFSLLQ
                     GRAERESRRAHGKGFTDDVVSAVERTASDSRMLRAQASEASASARGMLGKTSEVAAAA
                     EQSALAMRDAAETAAGLIRAIEDARREVELAGEVATRAGDQASDAARVSQALSGHVEA
                     IESILGLIRDIAGQTNLLALNATIEAARAGDAGRGFAVVAQEVKSLAAQTARATDDIT
                     MKITAIQQATRQTVEANGSIQKTVEDVQVSAERIREAMEVQAQTVTMITAAVDETALA
                     ADSMSATIAAIREDTENVAREIDGVTSNFGHVDEEMGRFKAAAAEFVKRFAA"
     gene            1841..>2238
                     /locus_tag="VGB57_09015"
     CDS             1841..>2238
                     /locus_tag="VGB57_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131534.1"
                     /translation="MADAIRHRDGQVLQKLTDEQVDHRLGAYNPDGEFERELGWLWAQ
                     AGDLIEAAVVASGGETAARRTRESFTRPVDSGWIHEVARLGFDIYARKTSVPREMDRR
                     ARLVASLCAGFRARFEDEAGFCRALEALQRL"
BASE COUNT          371 a          807 c          741 g          319 t
ORIGIN      
        1 catgctcttt ctcccgaccc gcacccaacg ccaagccttt gagattggtt cacttattaa
       61 ccatcttcct gagtcgggcg cctctcaacc ccgaagtcgc accaaccccc tctcccgctt
      121 ttcgcgggag aggaggggcc caagccgaag gcttgggagg tgagggtctc gccgagtccg
      181 gtgtacagac cctctcctcc caccgcttcg cggcggcccc tccactcccg cgaaaagcgg
      241 gagagggtga gaaggctccg gttcggcgcc cacccccttc cctaaggcga tgttaaccct
      301 ttgccgccag aaccggcgga taacctcagg cgcgggcgtg gaaggcatag taatgagcgc
      361 ggaattcacc caggtgatgg tggcggagcg gatcgggctg ttcgattcgg acggcagcct
      421 cgaggccaat tgccgcgagc tcgccgccct gctcgccaac gattcggtcc gggtcgcccg
      481 ccgcttctgg agccatctcg ccggggcgcc ggaggtcaat gccgctttct ccgaagccga
      541 gatggacgag aatgtccgca agatcctccc ttacatggcc gccaaatata ccgacgtccg
      601 gggccaggcc tgggccgaca tggtcggcgg ctatgtcctc gacgcgcaat cgacccatgt
      661 ctcgctgacc accctcctct cctcgatcgg cgccgccgcc gaggaagggc acgacatcct
      721 ggtcgacaag ctctcgggcg accccgagcg gctcggccgg ctcgcccgct gcctcaccaa
      781 ggcgaccttg ctcgaggcgg acatgttcgc cgcccatttc agccttctcc agggccgcgc
      841 cgagcgcgaa agccggcggg cccatggcaa gggcttcacc gacgacgtgg tcagcgcggt
      901 cgagcgcacc gcctccgaca gccggatgct ccgagcccag gcctcggaag cttcggcctc
      961 ggcccgcggg atgctcggca agacctccga ggtcgccgcc gcggccgagc aatcggcgct
     1021 ggcgatgcgc gacgccgccg agaccgccgc cggcctcatc cgggcgatcg aggatgcccg
     1081 ccgcgaggtc gagctggccg gcgaggtcgc cacccgcgcc ggcgaccagg cctcggacgc
     1141 cgcccgggtc agccaggctc tgtcgggcca tgtcgaggcc atcgaatcga tcctcggcct
     1201 gatccgcgac atcgccggcc agaccaacct cctcgccttg aacgccacca tcgaggcggc
     1261 ccgggccggc gacgccggcc ggggcttcgc cgtcgtcgcc caggaggtga agtcgctcgc
     1321 cgcgcagacg gcgcgcgcca ccgacgacat cacgatgaag atcacggcga tccagcaggc
     1381 cacccgccag acggtcgagg ccaacggctc gatccagaag acggtcgagg acgtccaggt
     1441 ttcggccgag cgcatccgcg aggcgatgga ggtccaggcc cagaccgtga ccatgatcac
     1501 cgccgcggtc gacgagaccg cgctcgccgc ggacagcatg tcggcgacca tcgcggcgat
     1561 ccgggaggac accgagaacg tcgcccgcga gatcgacggg gtgacgagca atttcggcca
     1621 tgtcgacgag gagatggggc gcttcaaggc cgccgcggcc gagttcgtga agcgcttcgc
     1681 cgcgtaaccg tcatcctggc gaaagccggg accccgggtc gagaagtctc ggcgggtctc
     1741 cccgaggtcc cggcttccgc cgggatgacg gagggtccta aaccttcctt taccgcgacg
     1801 atgatagggc tgtgccgagc aacctcgcat cggagcaccc atggccgacg caatccgcca
     1861 ccgggacgga caagtcttgc agaagctgac cgacgaacag gtcgaccacc gcctcggcgc
     1921 ctacaatccg gacggcgagt tcgaacggga gctcggctgg ctctgggccc aggcgggcga
     1981 cctgatcgag gcggcggttg tggccagcgg cggcgaaacc gcggccaggc gcacgcgcga
     2041 gagcttcacc cgcccggtcg actcgggctg gatccacgaa gtcgcccggc tgggcttcga
     2101 tatctacgct cggaaaacat cggtgccccg ggaaatggac cggcgggccc ggctcgtcgc
     2161 ctcgctctgc gccggcttcc gcgcccgctt cgaggacgag gccggtttct gccgggcgct
     2221 cgaggcgctc cagcggct
//
LOCUS       DASWNU010000460         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_43906, whole genome shotgun sequence.
ACCESSION   DASWNU010000460 DASWNU010000000
VERSION     DASWNU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43906"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..584)
                     /locus_tag="VGB57_09020"
     CDS             complement(327..584)
                     /locus_tag="VGB57_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131535.1"
                     /translation="MLKKALRLFVIKTRLEAWAIIYALAVGGVTRGLQYTVQYPGTGG
                     WLLFAACTGAVFMGGAKILDATRGPSHRRATLQRLVARARA"
     gene            complement(830..1603)
                     /gene="xth"
                     /locus_tag="VGB57_09025"
     CDS             complement(830..1603)
                     /gene="xth"
                     /locus_tag="VGB57_09025"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0131536.1"
                     /translation="MRIATFNVNGVNGRLAVLLEWLAEAKPDAVCLQELKAPDEKFPI
                     AAIEKAGYGAIWHGQQRWNGVAILGRDCEPVETCRGLPGDTGDVQSRYIEAAVCGVLI
                     GGLYAPNGNPHPGSKFDYKLAWNERLIDRAAELIGLGEPVVLAGDYNIIPTPTAIDAY
                     KPERWTDDALMQPEPQAQYRRLVGQGWTDALRHFHPDEPVFTFWDYWRNAFARNAGIR
                     IDHLLLNPPAAQRLKAAGVDREARAKEKASDHAPTWVEL"
BASE COUNT          317 a          595 c          645 g          342 t
ORIGIN      
        1 ccgccgacag gacgaccgcc aggaccgccg ccgccgcatt ctcgctgagc ttccgatagt
       61 tgaacatttt cctgctcctt gaaccttgtt gctcccccgc ccgaagcggt tgcagacatg
      121 attacaaggg ccgtgccaag tacgggaaat ggcggaaaac cgcgaaaaat ggggcggccg
      181 gcaccggcag acgggattta tcgccaccca ggtttgggaa attacgccaa cggttggggc
      241 gccgtcatcc cggcgaaagc cgggacctca ggtcgatgag tctcggcggg tctccccgag
      301 gtcccggctt tcgccggaat gacggatcag gccctcgccc gcgccaccag gcgctggagg
      361 gtggcgcggc ggtggctggg cccgcgggtc gcgtcgagga tcttggcgcc ccccatgaag
      421 accgcgccgg tgcaggcggc gaagaggagc cagccgccgg tgccgggata ttggaccgta
      481 tattggaggc ccctggtcac cccgccgacg gcgagcgcgt agatgatcgc ccaggcttcg
      541 agccgggtct tgatcacgaa aagtcttaag gccttcttca gcatgtccgc taatcagcaa
      601 gatgcgggcc agcggcggaa aaccgcggtt tttgggaatg ggtgggggtg gaagtgtaaa
      661 cgacggcgac gatttcctcc tttgattgct ccccggcatt tgccggggac ggcccgcgct
      721 aagcgcggcg gaggggttct tgaaaacagg gacagtccct ctttttctcg cgccgaaaag
      781 agggactgtc cctgattaca gctgcgccga aaagagggac tgtccctgat tacagctcca
      841 cccaggtcgg cgcgtgatcg ctggccttct ccttcgccct cgcctcccgg tcgactcccg
      901 cagccttcag ccgttgggcc gccggcgggt tgagaaggag atggtcgatc cggataccgg
      961 cgttgcgggc gaaggcgttg cgccaatagt cccagaaggt gaagacgggc tcgtcggggt
     1021 ggaaatggcg aagcgcgtcg gtccagccct ggccgacgag ccggcgatat tgggcctggg
     1081 gctcgggctg catcagagcg tcgtccgtcc accgctcggg cttgtaggcg tcgatggccg
     1141 tcggggtcgg gatgatgttg tagtcgccgg cgagaacgac cggctcgccg aggccgatca
     1201 gctccgccgc ccggtcgatc agccgctcgt tccaggcgag cttataatcg aatttggacc
     1261 cgggatgcgg gttgccgttc ggggcgtaga ggccgccgat cagcaccccg cacaccgccg
     1321 cctcgatgta gcggctctgg acgtcgccgg tgtctccggg aaggcctcgg caggtctcga
     1381 ccggctcgca gtcgcggccg aggatcgcga ccccgttcca gcgctgctgg ccgtgccaga
     1441 tggcgccgta gccggccttc tcgatcgcgg cgatcgggaa cttctcgtcg ggcgccttca
     1501 attcctgaag gcagaccgcg tccggcttcg cctccgccag ccattcgagc agcacggcaa
     1561 gccggccgtt gacgccgttg acgttgaaag tggcgatccg catcccgtcc gaaagcgtac
     1621 gctcgcgacc ggttccgaac gcgagcaaga gcggtcaacc cggccggaat ggcggatttc
     1681 cgccaagtct ttacgtggcg cggattttgc ttacgattac atttgtgatc gactcgacac
     1741 ctttttacct gatgcgcgac catggcgagg gcgagcgcct gccgctgaga cacggctgcg
     1801 tcgttctttc gaggctcgag ggcggccgca gcatggtccg ggcgctcgcc ccttcggtca
     1861 agttcgtgct gagcggcgag gaagtgtaca agatcgacg
//
LOCUS       DASWNU010000461         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_252079, whole genome shotgun sequence.
ACCESSION   DASWNU010000461 DASWNU010000000
VERSION     DASWNU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252079"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..141
                     /locus_tag="VGB57_09030"
     tRNA            67..141
                     /locus_tag="VGB57_09030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Thr,seq:ggt)
     gene            114..245
                     /locus_tag="VGB57_09035"
     CDS             114..245
                     /locus_tag="VGB57_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131537.1"
                     /translation="MSSILTGSTISSPNGDRPDPTADSAVAAHGWTMKSLLVIVSGA"
     gene            complement(381..2555)
                     /locus_tag="VGB57_09040"
     CDS             complement(381..2555)
                     /locus_tag="VGB57_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0131538.1"
                     /translation="MAIRTVGPNSTYPSIAAAMADSGPGDTIQLEAGYSNETATILHS
                     GMIVTGEASSTGIVLNLGTGIATVTLEGTAPINVNDASDGNGIVGNDGDNLVTVTAGA
                     DSASGGGGEDRLFVDYRLATGAVTGTLANVAEAGGGGRLVTINGGFEHLTIWTGSGAD
                     TITTGAGDDDIRTGEGAGTVTAGEGFNTIIGGSGADTITAGSGGNYVNAGDGANTITT
                     GDGEDEILTGTGADTIVSGGADDRITVTGGADSADAGAGSDLLIIDYSAAVTAVTGGV
                     TSGNLAAGYSGHIADLAGATLDFVGVETMYVTTGSGNDTVTTGGGNDRLVGGDGSDRF
                     FLHLGGDDFASGGTGNDIFLLGAAMTSADRIEGGDGTDTLVLQGDYAEGLVLSANVIE
                     IENVSFLAGSNTALGEPGGNRFDYALTTDDSNFAAGVQARINGSALLAGEDLTFDGSA
                     ETDARFVVYGGRGMDDLTGGAGNDIFFFAEGGRFAEGDTVDGGGGYDGLFLRGNYTID
                     FTQEGYAGALASVENLTVTGAGDERYARGGGTEFDYSITWDGDLLAGGLIMTVNGSTL
                     GAEESLAFNGSEETDGSFRLFGGFGNDVLTGGSGADTILGGARGDTLTGGAGNDVFRY
                     DKVEESNSTERDGIQDFNAGDVIDLSRIDANTLVAGNQAFSFIGGAAFSGTAGELRFE
                     NISLGGPVWMVQGDTNGDGVSDYEVVLVINPPDPITSSDFIL"
     gene            complement(2817..3323)
                     /locus_tag="VGB57_09045"
     CDS             complement(2817..3323)
                     /locus_tag="VGB57_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131539.1"
                     /translation="MIVRKAAVAALVVVAAAPFAASAQLSSYATETMSGQRLVAPPGA
                     MEVHVSRDQVPAGTTIAVHMHFWPRYVLVESGEVQVTLLDGGPMETFTAGETIVEPLE
                     KWHSGLVTKDAVLVAVEQTPPGGCNTIHPPAPAQPAVAARPAANGKPAVPARPAVPAM
                     SNDRCPRP"
     gene            complement(3382..3705)
                     /locus_tag="VGB57_09050"
     CDS             complement(3382..3705)
                     /locus_tag="VGB57_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0131540.1"
                     /translation="MSFTAELSTAPDDDGRRAARADVVLGAGLRQRGAHAVTVQVLDL
                     SITGFRAATHLELMNGTDIWLKLPGLESLHSRVVWMKGHLLGCEFVRPLHPAVLDMIV
                     RNAPR"
     gene            complement(3715..>4010)
                     /locus_tag="VGB57_09055"
     CDS             complement(3715..>4010)
                     /locus_tag="VGB57_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131541.1"
                     /translation="ALVECQTVPPVGTELMFSRGSTSIPARVAWTGADRVGLEFAYMI
                     DEHEVLVQLKRTTSDQNQPRFRRPRLFGEQMSQHEKQIARLWGASVGIAIPGE"
BASE COUNT          619 a         1314 c         1364 g          713 t
ORIGIN      
        1 catagcgccg cctctcccgc gggtcatcaa gctgccgctt cgatgaaaag ccccattggc
       61 gagtatgctg ccgtagctca gtggtagagc gcatccttgg taaggctgag gtcgtgagtt
      121 caatcctcac cggcagcacc atctcttctc cgaacggcga ccggccggat cccaccgcgg
      181 attcggccgt cgcggcccat ggctggacga tgaagtcgct gctcgtgatc gtatccggcg
      241 cgtagctccc gtcattccgg cgaaagccgg gacctcgggg agacccgccg agacttcttg
      301 tcctgaggtt ccggctttcg ggacgacgaa gggagagacg gccgcgcgag cagccgtccc
      361 cctttctcgt caggtgcagc ctaaaggatg aagtcgctgc tcgtgatcgg gtccggcgga
      421 ttgatcacca gcaccacctc gtagtcggac actccgtcgc cgttggtgtc gccctggacc
      481 atccagaccg gaccgcccag cgagatgttc tcgaagcgga gctctccggc cgttccgctg
      541 aaggcggcgc cgccgatgaa gctgaacgcc tggttgcccg cgacgagggt gttggcgtcg
      601 atcctcgaca ggtcgatcac gtcgccggcg ttgaagtcct ggatcccgtc gcgctccgtg
      661 gagttcgatt cctccacctt gtcatagcgg aagacgtcgt tgcccgcccc gccggtgagg
      721 gtgtcgcccc tcgccccgcc caggatcgtg tcggcgcccg agccgccggt cagaacgtcg
      781 ttcccgaagc cgccgaagag ccggaagctt ccgtccgtct cctccgaacc gttgaaggcc
      841 aggctttcct cggcccccag agtgcttccg ttgaccgtca ttatcaggcc gccggcgagc
      901 aggtcgccgt cccaggtgat ggagtaatcg aactcggtgc cgccgccgcg ggcgtaacgc
      961 tcgtcgccgg ccccggtgac cgtgaggttc tcgacgctgg cgagggcccc ggcatagcct
     1021 tcctgggtga agtcgatcgt gtaattgccg cgcaggaaga ggccgtcata gccgccgccg
     1081 ccatcgaccg tgtcgccctc ggcgaagcgg ccgccctcgg cgaagaagaa gatgtcgttg
     1141 ccggcgccgc cggtcaggtc gtccatgccc cggcccccgt agacgacgaa cctggcgtcg
     1201 gtttcagccg agccgtcgaa ggtcagatcc tcgccggcga gcaatgcgga accgttgatc
     1261 cgggcctgga cgcccgccgc gaaattcgaa tcatcggtgg tcagcgcgta atcgaagcgg
     1321 ttgccgcccg gctcgcccaa agcggtgttg ctgcccgcga ggaacgagac gttctcgatc
     1381 tcgatcacat tggcgctcag cacgagtccc tcggcataat cgccctgcag gaccagggta
     1441 tcggttccgt caccgccctc gatccggtcg gccgaagtca tggccgctcc aagcaggaaa
     1501 atatcgttgc cggtccctcc ggaagcaaaa tcgtcgccgc cgaggtgaag gaagaaacgg
     1561 tcgctgccgt cgccgcccac cagcctgtcg ttgccgccgc cggtggtgac cgtgtcgttg
     1621 cccgacccgg tggtgacgta catggtttcg acgccgacga aatcgagcgt tgcgccggcg
     1681 aggtcggcga tgtgcccgga atagccggcc gcgaggttgc cgctggtcac tccgccggtg
     1741 acggcggtga cggcggcgga atagtcgatg atgagcaggt cgctgccggc gccggcatcg
     1801 gcgctgtccg caccgcccgt caccgtgatc cggtcgtcgg cgccgccgct gacgatcgtg
     1861 tcggcgccgg tgccggtgag gatctcgtcc tcgccgtcgc cggtggtgat ggtattggcg
     1921 ccgtcgccgg cgttgacata attgccgccg ctcccggcgg tgatcgtgtc ggcgccgctg
     1981 cctccgatta tggtgttgaa gccctcgccc gcggtgaccg tcccggcccc ttcgccggtt
     2041 cggatatcgt cgtcgccggc tccggtcgtg atggtgtcgg cgcccgagcc ggtccagatg
     2101 gtgagatgct cgaagccgcc gttgatcgtc accagcctgc cgccgccgcc ggcctcggcg
     2161 acgttggcca gagtgccggt gaccgcgccc gtggcgagcc gatagtcgac gaacaggcgg
     2221 tcctcgccgc ctccgccgct cgccgaatcg gcgccggcgg tgacggtgac gagattgtcg
     2281 ccgtcattgc cgacgatgcc gttgccgtcg gaggcatcgt tgacgttgat gggcgcggtg
     2341 ccctcgagcg tcaccgtggc gatgcccgtg cccaggttca ggacgatgcc ggtcgagctc
     2401 gcctcgccgg tcacgatcat cccgctgtgg aggatggtcg cggtctcgtt cgaatagccc
     2461 gcctcgagct ggatggtgtc gcccgggccg gaatcggcca tggcggcggc gatggacgga
     2521 taggtgctgt tgggaccgac ggtgcggata gccatgaaat gtctcctggc ggagcgccag
     2581 gaagctgcat catcccggat cgcaaacgcg ttgtatgcac ggaaaacgcc gtggcgccat
     2641 tgcaggaata agtctctgac cgatcaagaa aaagatgatc tcagatgtag aaaagggcta
     2701 ggcgcaggcg ccctaacaaa caactaatta ttttcagata taattccaga agtctttgga
     2761 acttttccaa ataatcctgt cggggcctct atcctgcttt tgcccggcta tcgtgcttat
     2821 gggcgcgggc atctgtcgtt ggacatggcc ggaaccgccg gcctggcggg cacggccggc
     2881 ttgccgttcg cggccggcct ggcggccacg gccggctggg ccggcgcggg cgggtggatc
     2941 gtgttgcagc ctcccggcgg ggtctgctcg acggcgacca gcaccgcgtc tttcgtgacc
     3001 agtccggaat gccatttttc gagtggctcg acgatcgtct cgccggctgt gaaagtctcc
     3061 atgggacctc cgtcgaggag ggtgacctgg acctcgccgc tctcgacgag gacgtagcgg
     3121 ggccagaaat gcatgtgcac ggcgatcgtc gtcccggcgg ggacctggtc ccggctaacg
     3181 tgcacctcca tcgcgcccgg cggcgccacg agccgctggc cggacatggt ttcggtcgca
     3241 tagctggaca attgggccga ggccgcgaac ggcgcggcgg cgacgacgac gagcgcggcc
     3301 acggccgctt ttcttacgat catttcggtt ctcccccact gattgggaga caatgcgcct
     3361 gtcggggctt ggcgtccacg cttaacgagg agcgttccgg acgatcatgt cgaggaccgc
     3421 cgggtggagc gggcggacga actcgcagcc gagcagatgg cccttcatcc agacgacgcg
     3481 ggaatggagc gattcgagcc cgggcagctt cagccagatg tcggtgccgt tcatcagctc
     3541 gagatgggtg gcggcgcgga agccggtgat ggagaggtcg aggacctgga ccgtgaccgc
     3601 gtgggcgccg cgctgccgga ggccggcgcc gagcacgacg tcggcccggg cggcgcggcg
     3661 gccgtcatcg tccggagcgg tcgaaagctc ggcggtgaag ctcatcggcg ggcctcactc
     3721 ccccgggatg gcgatgccca ccgaggcacc ccacaggcgg gcgatctgct tctcatgctg
     3781 gctcatctgc tcgccgaaca ggcgcggtcg gcggaagcgg ggctggttct ggtcgctcgt
     3841 ggtgcgcttc aattgcacca gcacctcatg ctcgtcgatc atgtaggcga attcgaggcc
     3901 gacccggtcc gcgcccgtcc aggccaccct ggccgggatg ctggtcgagc cgcgactgaa
     3961 catgagctcg gttccgaccg gcggcaccgt ctggcattcg accagggcgc
//
LOCUS       DASWNU010000462         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_43963, whole genome shotgun sequence.
ACCESSION   DASWNU010000462 DASWNU010000000
VERSION     DASWNU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43963"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGB57_09060"
     CDS             <1..379
                     /locus_tag="VGB57_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0131542.1"
                     /translation="EAIAWFTGKLGFTLVEDSRQPEQDKRWVLIAPPGAPRGSASILL
                     ARASTPEQAAFIGDQAGGRVFLFLRTDDFDRDHAAMRGKGVAFVREPMQAPYGKVAVF
                     EDLYGNLWDLVQFADRGLGASPL"
     gene            494..1306
                     /locus_tag="VGB57_09065"
     CDS             494..1306
                     /locus_tag="VGB57_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0131543.1"
                     /translation="MNPNKVLWEKGDFTRIAGTMRESGEALVAALGIGPGLNMLDLGC
                     GDGTTALPAARAGADVLGVDIAANLVAAGRARAASAGLANLRFEEGDASNLIELADRR
                     FDLVLSMFGAMFAPRPFDVAREMVRVTRPGGRIVMGNWIPGDPTLVARILKISAAFAP
                     PPPEGFVSPVAWGVEEHVRERFAAAGVAPEDIAFERCTWLFRHSGPPAEFLDMFRHYY
                     GPTMNAFDAAAKDGREDKLQAELDDLFEAQNRAGPERTEIPATYLKVTVTKA"
     gene            1393..2148
                     /locus_tag="VGB57_09070"
     CDS             1393..2148
                     /locus_tag="VGB57_09070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY0131544.1"
                     /translation="MAEADAFPLRLRNAEEYARLIDDDVHVFGKGHRCRTDKRGRPTP
                     KGRSLFEIRVDATGGFVPLWEQNRILRYRFDARSLNRFEDPEALAARIRQLFGEGLAA
                     WGDSAPIRFAERAETCDFEFVVRNADDCDDTGCVLASTFFPDQGQHEFVIYPEMFNQT
                     REEQVETMVHEIGHIFGLRHFFADVAEDAWPSELFGEDSRFSIMNYGANSYLTNADRA
                     DLKALYGAVWSGRLDNVNGTPIRQVRPYSSLRP"
     gene            2198..2599
                     /locus_tag="VGB57_09075"
     CDS             2198..2599
                     /locus_tag="VGB57_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131545.1"
                     /translation="MSLWSWIIALAALLFSIGCFCLFVLSATRTPVPDKVVEDAAKRA
                     EVRSLSPAALGALVKALADAFSKVGPSALALIGAILFLLLAGEASEVFHLTDAPEKAQ
                     PTKPRAGEQNRSAGSTAGNESREADPAANGQ"
BASE COUNT          454 a         1046 c          942 g          451 t
ORIGIN      
        1 cgaggcgatc gcctggttca ccggcaagct cggcttcacc ctcgtcgagg acagccgtca
       61 gcccgagcag gacaaacgct gggttctgat cgcgccgccc ggcgctccgc gaggctcggc
      121 ctcgatcctt ctcgcccgag cttccacccc cgaacaggcg gcgttcatcg gcgaccaggc
      181 gggcgggcga gtcttcctgt tccttcgcac cgacgatttc gaccgcgacc acgcggcgat
      241 gcgcggcaag ggcgtcgcct tcgtccgcga gccgatgcag gcgccctacg gcaaggtcgc
      301 ggtgttcgag gacctttacg gcaatctgtg ggatctcgtg caattcgccg atcggggcct
      361 tggcgcctcc cccctgtaac aatcgcaacc cctttgccgt cgccgcgaca tcggaccggc
      421 acaggctgag tccaggttcg gcctcttgcg cggcccccgg ccggcccccg atctcattgg
      481 aaggaaagcg gcgatgaacc cgaacaaggt cctttgggaa aaaggcgact tcacccggat
      541 cgccggcacc atgcgcgaga gcggcgaggc gctggtcgcc gcgctcggca tcggacccgg
      601 cctcaacatg ctcgatctcg gctgcggcga cggcaccacc gcgctcccgg ccgcccgcgc
      661 cggagccgac gtgctcggag tcgacatcgc cgccaatctc gtcgccgcgg gcagggcgcg
      721 cgccgcctcg gccggcctcg ccaatcttcg tttcgaggag ggcgacgcgt ccaatctcat
      781 cgagctcgcc gaccgccgct tcgatctcgt cctcagcatg ttcggagcga tgttcgcccc
      841 gcgccccttc gacgtggcgc gggagatggt gcgggtgacc cgccccggcg gccggatcgt
      901 catgggcaat tggatcccgg gcgatcccac cctcgtcgcc cggatcctga agatcagcgc
      961 cgctttcgca ccgccgccgc cggaaggctt cgtcagcccg gtcgcctggg gcgtcgagga
     1021 gcatgtccgc gaacgcttcg ccgccgccgg agtcgctccg gaggatatcg ccttcgagcg
     1081 ctgcacctgg ctgttccgcc actccggccc gccggccgag ttcctcgaca tgttccgcca
     1141 ttattacggc ccgaccatga acgccttcga cgccgcggcg aaggacggcc gcgaggataa
     1201 gctccaggcc gagctcgatg atctgttcga ggcgcagaac cgggcggggc cggagcgcac
     1261 cgaaatcccg gccacctacc tcaaggtgac ggtcacgaaa gcctgatcgg ggccgctggg
     1321 cgtgatccgc gtcacgctcg gcgggagccg acgggcttat ccaggtgggc gacgctcacc
     1381 aggagaccgg tcatggccga agccgacgcc ttccctttac gccttcgcaa cgccgaggaa
     1441 tatgcgcgcc tgatcgacga cgacgtccat gtcttcggca aggggcaccg ctgccgtacc
     1501 gacaagcgcg gccggccgac cccgaagggc cgttcgctgt tcgagatccg ggtcgacgcg
     1561 accggcggct tcgtcccgct ctgggagcag aacaggatcc tgcgctaccg cttcgacgcg
     1621 cgttcgctca accggttcga ggatcccgaa gccctggcgg ccaggatcag gcaattgttc
     1681 ggcgaaggcc tcgccgcctg gggggactcg gcgccgatcc gcttcgccga gcgggccgaa
     1741 acctgcgatt tcgagttcgt cgtccgcaac gccgacgatt gcgacgacac cggctgcgtc
     1801 ctcgccagca ccttcttccc ggaccagggc cagcacgagt tcgtcatcta cccggaaatg
     1861 ttcaaccaga cccgcgagga gcaggtcgag accatggtcc acgaaatcgg ccatatcttc
     1921 gggcttcgcc acttcttcgc cgacgtcgcg gaggacgcct ggccgtcgga gctgttcggc
     1981 gaggacagca ggttctcgat catgaattac ggcgcgaaca gctatctcac caatgccgat
     2041 cgagcggatt tgaaagctct ctacggcgcg gtctggagcg gtcggctgga caatgtgaac
     2101 ggcacgccga ttcgccaggt gcggccctac agctccctca ggccgtgagc ctgtcccatc
     2161 ccgctgcttg caacgtcttt gaaagaaggg gagcgtcatg agtctatggt cgtggatcat
     2221 cgcactggcc gcattgctgt tctcgatcgg ctgcttctgc ctgttcgtcc tcagcgccac
     2281 ccggacgccg gtacccgaca aggtcgtcga ggacgccgcc aagcgggccg aggtccgaag
     2341 cttgagcccg gcggcgctcg gcgccctggt gaaggctttg gccgacgcct tttcgaaggt
     2401 gggaccctcc gccctggcgc tgatcggagc catcctgttt ctccttctcg ccggtgaagc
     2461 gagcgaggtc ttccatctca ccgacgcgcc ggagaaggcc cagccgacca agcccagggc
     2521 cggggagcaa aaccggtcgg cgggctcgac cgccgggaac gagagccgcg aagcggatcc
     2581 ggccgccaac gggcaatgaa ttcggcccgc gcggccgggt ccggcggccc ggccgcgcca
     2641 cgcccgttcc gggccccgcc acgtccgtgc aacgagcggc cgcgagcccg cccttgacgc
     2701 gaggcccacg cggtgggacc cagcggacgg ggaggacggc cgacatgcgc atcctgggac
     2761 tgatcgccgc gacatcgact ctgctcgccg cctgcgggag ccgcgactcc ggccctgtcg
     2821 ccgagcaggg caacgaaacc cagccgtccg ccgccaatgc cgcggctccc gtcgcggcca
     2881 gtcccgccgc cga
//
LOCUS       DASWNU010000463         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_96090, whole genome shotgun sequence.
ACCESSION   DASWNU010000463 DASWNU010000000
VERSION     DASWNU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96090"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1049
                     /locus_tag="VGB57_09080"
     CDS             108..1049
                     /locus_tag="VGB57_09080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0131546.1"
                     /translation="MPELPNPIYWSIPGFVGLLLLEFWVARRQRRRLHEGKDTLTSLG
                     LGFGSLLAGILSAGLVAAVSIWVYRFHLIDIGYAWWAFVLCFLLDDLAYYAFHRSAHR
                     VRWFWASHVVHHSSERYNFSTALRQTWTGFLSLNFLFRLPLFLIGFPPAMIFFCVGLN
                     LVYQYWIHTETIRRMPRWFEAVMNTPAHHRVHHAVNPRYLDRNYAGVFIIWDRMFGSF
                     EPERDEDPPRYGIVKPLGSYNLFWAALHEWVEMGRDAWRAPGLKAKLAYLVREPGWSH
                     DGSRETSAMIRARWREGSVIPAEAGTSSGEKLGANSR"
     gene            complement(1079..1990)
                     /locus_tag="VGB57_09085"
     CDS             complement(1079..1990)
                     /locus_tag="VGB57_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0131547.1"
                     /translation="MEVALVAEGLEFPEGPVTMADGSVIVAEIKGRRITRVLADGSKE
                     VLAETGGGPNGLAIGPDGALWIADNGGAFSWHEEMGLTIPGPTPPDHEGGSIRRFDFA
                     SGRLETVYESCDGRRLIGPNDLVFDWEGGLWFTDHGCTTADGRRFGALCYASADGSRI
                     TRQRDGLLAPNGVGLSPDGRTVYVADTWLQRLWAFDVETPGVLAAPSPIAPGRVVANL
                     PGYQLLDSLAVEADGRVCVATLVNGGITAIEPDGAIEHFPVPDPICTNICFGGDDMRD
                     AWITASGTGRLYKTRWPRPGLKLAFNA"
     gene            complement(1990..>2728)
                     /locus_tag="VGB57_09090"
     CDS             complement(1990..>2728)
                     /locus_tag="VGB57_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0131548.1"
                     /translation="IAVAKAAADTVKRVHQELGGKSPFIVLEDADVGKAVTAAMRHML
                     TNSGQSCNAPTRLLVPKARMEEAKEAARAVAEKTKVGDPKGDANLGPVVSDVQWTKIQ
                     GLIERGVSEGATLVVGGPGKPDGLERGYYVRPTVFGDVDNDMAIAREEIFGPVMSIIG
                     YEDEEQAVEIANDTPYGLAAYVHSSDTGHARKVAARLRAGQVNINGAEVDFMAPFGGY
                     KQSGNGREWGDHAFEEFLETKAILGHA"
BASE COUNT          392 a          923 c          939 g          474 t
ORIGIN      
        1 tcattgcgag gagcgcagcg acgcggcgac cccagcttcg agcgccagcg ccgctggatt
       61 gcttcgctcc gctcgcaatg acgaaggttc cgcgataggc tccacccgtg cccgagcttc
      121 ccaaccccat ctactggtcg atcccgggct tcgtcgggct gctgctgctc gaattctggg
      181 tggcgaggcg gcagcgaagg cggctgcacg aggggaagga caccctcacc tcgctcggtc
      241 tcggcttcgg cagccttctg gccgggattc tgtccgccgg gctggtcgcg gccgtctcga
      301 tctgggtcta ccgcttccac ctgatcgaca tcggctatgc ctggtgggcc ttcgtcctgt
      361 gcttcctgct cgacgacctc gcttattacg ccttccatcg ctcggcgcac cgggtgcgct
      421 ggttctgggc aagccatgtc gtccaccact cgtccgagcg ctacaatttc tcgaccgcgc
      481 tgaggcagac ctggaccggc ttcctcagcc tcaatttcct gttccgcctg ccgctgttcc
      541 tgatcggctt cccgccggcg atgatattct tctgcgtcgg gctgaacctc gtctaccaat
      601 attggatcca cacggagacg atccggcgga tgccgcgctg gttcgaggcg gtgatgaaca
      661 cgcccgccca ccaccgggtg caccacgccg tcaacccgcg ctacctggac cgcaattatg
      721 ccggcgtctt cataatctgg gacaggatgt tcggaagctt cgagccggag cgggacgagg
      781 acccgccgcg ctacggcatc gtgaagccgc tgggctccta caacctcttc tgggcggcgc
      841 ttcacgaatg ggtggagatg ggccgcgacg cctggcgcgc gccggggctg aaggcgaagc
      901 tggcctatct cgtccgcgaa cccggctgga gccacgacgg ctcgcgcgag accagcgcca
      961 tgatcagggc tcggtggcgg gaaggctccg tcatcccggc ggaagccggg acctcatcgg
     1021 gtgaaaagct cggtgccaac tctcgctgag gtcccggctt tcgccgggat gacgggcgtc
     1081 aggcgttgaa cgccagcttc agccccggcc tcggccacct cgtcttgtag agcctgccgg
     1141 tgcccgacgc ggtgatccag gcgtcgcgca tgtcgtcgcc gccgaagcag atgttggtgc
     1201 agatcgggtc ggggaccggg aaatgctcga tcgctccgtc cggctcgatc gcggtgatcc
     1261 cgccgttgac gagggtggcg acgcagaccc ggccgtcggc ttcgacggcc agactgtcga
     1321 ggagctggta accgggcaga ttggcgacca cccggcccgg cgcgatcggc gacggcgcgg
     1381 cgagaacgcc gggcgtctcg acgtcgaagg cccagagccg ctggagccag gtgtcggcga
     1441 cgtagacggt ccggccgtcg ggcgagaggc cgactccgtt cggggcgagc agcccgtcgc
     1501 gctgacgggt gatgcgcgag ccgtcggccg aggcgtagca aagcgctccg aagcgccggc
     1561 catccgcggt cgtgcagcca tggtcggtga accagaggcc gccctcccag tcgaagacga
     1621 ggtcgttggg gccgatcagg cggcggccgt cgcagctctc gtagaccgtc tcaagccggc
     1681 cgcttgcgaa gtcgaagcgg cggatcgagc cgccctcatg gtccggcgga gtagggccgg
     1741 ggatggtgag gcccatttct tcatgccacg aaaaggcgcc gccattgtcg gcgatccaca
     1801 gcgctccgtc ggggccgatg gcgagtccgt tggggcctcc gccggtctcg gccagcacct
     1861 ccttcgaacc gtcggcgagc acccgggtga tccgccggcc cttgatctcg gcgacgatga
     1921 cggagccgtc cgccatggtc accgggcctt cgggaaactc gagcccctcg gcgacgagcg
     1981 cgacctccat caggcgtggc cgaggatcgc cttcgtctcg aggaattcct cgaaggcgtg
     2041 gtcgccccat tcgcggccgt tgcccgactg cttgtagccg ccgaagggcg ccatgaaatc
     2101 gacctcggcg ccattgatgt tgacctggcc ggcgcggagc cgggccgcga ccttgcgggc
     2161 atggccggtg tcggaggaat ggacgtaggc ggcgaggccg tagggtgtgt cgttggcgat
     2221 ctcgaccgcc tgctcctcgt cctcatagcc gatgatcgac atgaccgggc cgaatatctc
     2281 ctcgcgggcg atggccatgt cgttgtcgac gtcgccgaag acggtcgggc ggacgtaata
     2341 gccgcgctcc agcccgtcgg gcttgccggg gccgcccacg acgagggtgg cgccctcgga
     2401 caccccgcgc tcgatcaggc cctggatctt ggtccactgg acgtccgaga cgaccgggcc
     2461 gagattggcg tcgcctttgg gatcgccgac cttggtcttc tcggcgacgg ccctcgcggc
     2521 ttccttcgcc tcctccatcc gcgccttggg gacgaggagg cgggtggggg cgttgcagga
     2581 ctggccggag ttggtcagca tgtggcgcat cgccgcggtc accgccttgc cgacgtcggc
     2641 atcctcgagg acgatgaagg gcgacttgcc gccgagctcc tggtggaccc gcttcaccgt
     2701 gtccgcggcc gccttggcga ctgcgatt
//
LOCUS       DASWNU010000464         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_434644, whole genome shotgun sequence.
ACCESSION   DASWNU010000464 DASWNU010000000
VERSION     DASWNU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434644"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1503)
                     /locus_tag="VGB57_09095"
     CDS             complement(112..1503)
                     /locus_tag="VGB57_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0131549.1"
                     /translation="MIRKLFTAASLLALAACTTVGPNYESPAPAAPAQIGFVGASSPA
                     FTGDEPPGRWWSLLRDPVLDGLVEQALTANTDLRVAAANLAQARAVLREARAGRLPTT
                     NLSGGASFANEPGPGGADTIFDVGLDVGYQLDLFGRIRRATEASRADVEAVQAAFDLT
                     RITVAAETARAYSDACNFGRQLEVARDSVRIQERTFDLTRRRVEGGRGTALDTAQAGS
                     LLEQTRAQLPTLEGERRAALYRLSVLTGRPPAEFPQAVAACTTPPALTQPIPVGNGAS
                     LLARRPDIRRAERELAASTARIGVATAELYPDISLGLSAGSTAGSLGGLASGSGLRFG
                     LGPLISWTFPNRAVARSRIAQAEAATQAALARFDGSWLGALEETESALTRYANELDRV
                     ATLRRARANSAEAARIARLRYEAGREGFQLVLEAERSLAQTEAALAQAEAQRSTHLIS
                     VFLALGGGWQQSA"
     gene            complement(1500..>4482)
                     /locus_tag="VGB57_09100"
     CDS             complement(1500..>4482)
                     /locus_tag="VGB57_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0131550.1"
                     /translation="SSSSTNGAAGITVTFEPGTNLDAAQVLVQNRVALAEPRLPEQVR
                     QIGVTVNKASTGFLMIVALTSTDPNLDVDYIGNFANTNLRDRLLRLEGVGGVQVFGGG
                     NYAMRIWIDPDRAAARNLTAGEIVTALRTQNVQVAGGALGEPPYANGNPAFQLPIEVQ
                     GRLNDPAQFADVVIKTDASGAITRVRDVGRVELGSQEYGIRGSFTGKRGVALAIIQQP
                     GSNALDAAELVLNEVKEASKTFPPGIQYSIPYNPTEYVAASVEGVQETLIEAILLVVL
                     VVMIFLQTWRAAIIPILAIPVALIGTFAIQLALGFSINSLSLFALVLAVGIVVDDAIV
                     VVEAVEKHIRGGLTPREAAHRTMREVSGALIAIGLVLTAVFIPTAFVSGIPGIFYRQF
                     AVTIAAASLISLVVSLTLSPAMAALLLKPHKEHHSEGNRFTRPLRWAAGKFNAGFDWL
                     SDRYGRFTARVVRKSAIMLAVYSGLLALTGVTLTSTPTGFIPAQDQGVLIGVVQLPPG
                     SSLERTSEVLDRAYQIVRKTHGVVDAATFAGLDGASFSQSSNAGTMFIKLSDWADRSG
                     NALSADELSKTLTMALGGAIEEANVFIIAPPAVPGLGTGNGFTMMIQDRTGAGYQALE
                     GATFAMMGAAAQKQEVTQVFSLFNTGSPRVQADVDRDRAQLLGVQPAQVYEALGTYLG
                     STYVNDFNLLGRTFRVTAQAEPTARDDLSDIGRLQVRSATGAMVPLSSVATLRNDSGP
                     SRVVRYNLFPAVELQGAAAPGVSSGDALRVMDELAGQALPQGMSYEWTGLALQEQSAG
                     SSSTVIFLLAVVFVFLVLAAQYEALTLPLAVILIVPMCILAALAGVLLRGMDNNILTQ
                     VGLVVLVALAAKNAILIVEFAKQAEEEHGMDRFDAAVSAASSRLRPILMTSFAFIFGV
                     IPLAIAVGPGAEMRQALGTAVSFGMLGVTIFGLIFTPVFYVVARGLGRFLPRKPEKET
                     IYPTSYGGPARDPLQGDGATS"
BASE COUNT          735 a         1462 c         1614 g          671 t
ORIGIN      
        1 gctgcatcgg cctgaaacgt gaatcttgat ctcgactatc taatagaccg tgattcaaag
       61 ccatcggtga aagcgcgaaa cgcttccacc tcagacgatc acgctctcgc ctcaggcgct
      121 ttgctgccag ccgccgccga gggcgaggaa gaccgagatc aggtgggtgg agcgctgagc
      181 ttccgcctgg gcgagcgcgg cttcggtctg ggcgagcgag cgttccgcct cgaggacgag
      241 ctgaaaccct tcccggcccg cttcgtacct gagccgggcg atcctggcgg cttccgcgct
      301 gttggcgcgg gcacggcgca gggtcgccac ccggtccagc tcgttggcgt agcgggtgag
      361 cgcgctctcc gtctcctcga gagcgccgag ccagcttcca tcgaagcggg caagcgccgc
      421 ctgggttgcg gcctcggcct gggcgatccg gctgcgcgcc accgccctgt tgggaaacgt
      481 ccagctgatc agcggcccaa gcccgaagcg aaggcccgag ccggaggcga gcccgccgag
      541 cgagccggcg gtcgagccgg cggacaggcc gaggctgatg tcgggataga gctcggccgt
      601 ggcgactccg atgcgcgctg tggaggcggc gagctcgcgc tcggcgcggc ggatgtcggg
      661 ccgcctggca aggagcgagg cgccgtttcc gaccggaatc ggctgagtca aagccggagg
      721 cgtggtgcac gcggcgaccg cctgcgggaa ttcggcgggt ggacggccgg tcagcaccga
      781 gagccgatag agggccgcgc gccgttctcc ctccagcgtc ggaagctggg cccgggtctg
      841 ctcgagcagc gagcccgcct gggcggtgtc gagcgcggtg ccgcgcccgc cttcgacccg
      901 cctgcgggtg aggtcgaagg tccgttcctg gatccggacg ctgtcgcgcg cgacttcgag
      961 ctgtcggccg aagttgcagg cgtcggaata agcccgtgcc gtctccgcgg cgacggtgat
     1021 ccgggtgaga tcgaaggcgg cctgcaccgc ttcgacatcg gctcggctgg cttcggtggc
     1081 gcggcggatg cgcccgaaca ggtcgagctg gtaacccacg tccagcccga cgtcgaagat
     1141 cgtgtcggcg ccgccggggc cgggctcgtt cgcgaagctc gctccgcccg aaagattggt
     1201 ggtcggaagg cgccctgccc tggcctcccg aagcacggcg cgcgcctggg ccaggttcgc
     1261 ggcggccacg cgcaggtcgg tattggccgt cagggcctgc tcgaccaggc cgtcgagcac
     1321 cggatcccga agcaggctcc accagcggcc gggcggctcg tccccggtga aggcgggcga
     1381 ggaggcgccg acgaagccga tctgcgccgg cgcggcgggc gcgggcgatt cgtaattcgg
     1441 cccgacggtg gtgcaggcgg cgagcgcgag caggctggcg gcggtaaaga gcttgcggat
     1501 catgacgtgg ctccgtcgcc ctgcagcggg tcgcgcgcgg ggccgccgta ggacgtgggg
     1561 tagattgtct ccttctccgg cttcctggga aggaagcggc cgagcccgcg ggcgacgacg
     1621 tagaagaccg gcgtgaagat gagcccgaag atcgtcactc ccagcatccc gaagctgacc
     1681 gcggtgccca gtgcctgccg catctcggcg cccgggccga ccgcgatggc gagcgggatg
     1741 actccgaaga tgaaggcgaa gctggtcatc aggatcggcc tgaggcgcga gctcgcggcg
     1801 cttaccgcgg cgtcgaagcg gtccatgcca tgctcctcct ccgcctgctt cgcgaactcg
     1861 acgatcagga tggcgttctt ggcggcgagc gcgaccagca cgacgagacc gacctgcgtg
     1921 aggatgttgt tgtccatccc cctcagaagc acgcccgcga gcgcggcgag gatgcacatt
     1981 ggaacgatca ggatcaccgc cagaggcagg gtcagggcct cgtactgggc ggccagcacc
     2041 aggaagacga agaccaccgc cagaaggaag atgacggtcg agctgcttcc cgccgactgt
     2101 tcctgcaagg caaggccggt ccactcgtag ctcatgccct gcggaagcgc ctggccggcg
     2161 agctcgtcca tcacgcgcag ggcgtcgccc gaggaaacgc cgggcgctgc cgcgccctga
     2221 agctccaccg cggggaacag attgtagcgc accacccgtg aggggccgct gtcgttgcgc
     2281 aaggtggcga ccgaggacag aggcaccatc gcgccggtcg ccgagcgcac ctgaagccgt
     2341 ccgatgtcgg aaagatcgtc gcgggcggtc ggctccgcct gcgccgttac ccgaaaggtt
     2401 cggccgagca ggttgaaatc gttgacgtag gtagagccca gataggtgcc gagcgcctcg
     2461 tagacctgcg ccggctgcac tcccaggagc tgcgcccgat cccggtccac atccgcctgc
     2521 acgcgcgggg agccggtgtt gaacagggag aagacctggg tcacctcctg tttctgggcg
     2581 gccgcgccca tcatcgcgaa ggtcgcgccc tcgagcgcct ggtagcccgc gccggtgcgg
     2641 tcctggatca tcatggtaaa gccgttgccg gtgccgaggc ccggcacggc gggcggcgcg
     2701 atgatgaaga cgtttgcttc ctcgatcgcc ccgcccagcg ccatggtcag ggtcttggac
     2761 aattcatcgg cggaaagggc gttgccgctg cggtcggccc agtcgctgag cttgatgaac
     2821 atcgtgccgg cgttggacga ttgcgagaag ctcgccccgt cgagcccggc gaaagtggcc
     2881 gcgtcgacca ccccgtgggt cttgcggacg atctggtagg cccggtcgag cacttcgctg
     2941 gtccgctcta gcgaggagcc gggcggaagc tggaccactc cgatcagaac gccctggtcc
     3001 tgagcgggga tgaagccggt cggggtggag gtgagagtca ctccggtcag cgccagcagg
     3061 ccggaataga cggcgagcat gatcgcggac ttcctcacca cccgagcggt gaagcgcccg
     3121 tagcggtccg agagccagtc gaagccggca ttgaacttgc ccgccgccca gcgcaacggc
     3181 ctggtgaagc ggttgccctc gctgtgatgc tccttgtggg gcttgaggag caaggcggcc
     3241 atggccggag acagcgtcag cgatacgacc agcgagatga gcgaggccgc cgcgatcgtc
     3301 accgcgaact gccggtagaa gatgccggga atgcccgaga cgaaggcggt ggggatgaac
     3361 accgcggtca gcaccagccc gatcgcgatc agggcgcccg acacttcgcg catggtccgg
     3421 tgcgccgcct cgcgcggggt gaggccgcct cggatatgct tctccaccgc ctcgaccacc
     3481 acgatggcgt cgtcgacgac gatccccacg gccagcacga gcgcgaacag agagagcgag
     3541 ttgatcgaga agccgagcgc gagctggatc gcgaaggtgc cgatcagggc gaccgggatg
     3601 gcgaggatcg ggatgatcgc cgcccgccag gtctggagga agatcatgac gacgaggacg
     3661 acgaggagga tcgcctcgat cagcgtctcc tgaacgccct ccaccgaggc ggcgacatat
     3721 tcggtcggat tgtaggggat cgaatattgg atgccgggcg ggaaggtctt cgaagcctcc
     3781 ttgacctcgt tcagcaccag ctcggccgcg tcgagcgcgt tcgagcccgg ctgctggatg
     3841 atcgccagag ccactccgcg cttgccggtg aaggagccgc gaatgccata ttcctggctt
     3901 ccgagctcga cccggccgac gtcgcggacc cgggtgatgg caccgctcgc atcggtcttg
     3961 atgacgacat cggcgaactg cgccgggtcg ttcaaccgtc cctgcacttc gatgggaagc
     4021 tggaaggcgg ggttgccgtt cgcataaggc ggctcgccga gcgctccgcc cgcgacctgg
     4081 acgttctggg tgcggagcgc cgtgacgatc tcgccggcgg tgaggttgcg cgccgcggcg
     4141 cgatccggat cgatccagat ccgcatcgca taattgccgc cgccgaacac ctgcactccg
     4201 cccacgcctt cgagccggag cagccggtcg cgaaggttcg tgttggcgaa attgccgata
     4261 taatcgacgt cgaggttcgg atcggtcgag gtcagcgcca cgatcatcag gaacccggtc
     4321 gacgccttgt tcaccgtcac tccgatctgg cgcacctgct cgggcaggcg cggctcggcg
     4381 agggcgacgc gattctggac cagcacctgg gcggcatcca ggttggtgcc cggctcgaac
     4441 gtgaccgtga tccccgccgc gccgttggtg gacgaggaag ac
//
LOCUS       DASWNU010000465         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_44045, whole genome shotgun sequence.
ACCESSION   DASWNU010000465 DASWNU010000000
VERSION     DASWNU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44045"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="VGB57_09105"
     CDS             complement(<1..1904)
                     /locus_tag="VGB57_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0131551.1"
                     /translation="MNVQAPRDFIDRRQLLSALRSFRRGDFTVRLNEDLPGEDGEIAR
                     LFNETVSLNEQMTDELGRLSQVVGKEGKISQRGRVKAATGGWESAIRSVNELIEDMVQ
                     PTAEVARVIGAVAKGDLSQSMTVEIDGRPLRGEFLRIGKIVNTMVEQLASFASEVTRV
                     AREVGTEGKLGGQANVKGVAGTWKDLTDNVNAMATNLTGQVRNIAEVTTAVASGDLSK
                     KITVEVKGEILELKNTINTMVDQLNGFASEVTRVAREVGSEGKLGGQAQVEGVAGTWA
                     DLTDSVNLMAGNLTGQVRNIAEVTTAVAKGDLSKKITVDVKGEILELKNTINVMVDQL
                     NSFASEVTRVAREVGTEGKLGGQAQVPGVGGTWKDLTDNVNAMAANLTGQVRNIAEVT
                     TAVALGDLSKKITVDVKGEILELKNTINTMVDQLNSFASEVTRVAREVGTEGKLGGQA
                     QVRGIAGTWADLTDNVNLMAANLTGQVRNIADVTTAVARGDLSKKITVDVKGEILELK
                     NTINVMVDQLNGFASEVTRVAREVGTEGKLGGQAQVPGVGGTWKDLTDNVNLMATNLT
                     NQVRGIADVVTAVAQGNLKRKLTVDAKGEIAALAETINFMIETLSTFGDQVTNMAREV
                     GIEGRLGGQAR"
     gene            2131..2502
                     /locus_tag="VGB57_09110"
     CDS             2131..2502
                     /locus_tag="VGB57_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131552.1"
                     /translation="MPSDEDVSTFIRSTFRSVWALELLCFLRQDRSRALSHAEMVAGL
                     RGSDLVVTQSVDSLTAAGLVVGEGGGSARYNPASGELDRLVERAAALYARSPDAVRRT
                     IVAAANPSITAFADAFKLRKD"
     gene            2507..2779
                     /locus_tag="VGB57_09115"
     CDS             2507..2779
                     /locus_tag="VGB57_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY0131553.1"
                     /translation="MATWFPASVYLLCFMTSAACAILLTRSYRRTGARLLFWSGLCFL
                     FLAANNLVVIFDLLLLPEVDFRLARYLLALAALGVLLFGFIWDLED"
     gene            2780..3043
                     /locus_tag="VGB57_09120"
     CDS             2780..3043
                     /locus_tag="VGB57_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY0131554.1"
                     /translation="METLFDFLSGAITAGFAVAGFFFLRFWKRTRESLFLAFAAAFWL
                     LGLGQALLTFTDIPVEERSWLFLLRLAAFSLILVSIWRKNRRA"
     gene            3040..>3933
                     /locus_tag="VGB57_09125"
     CDS             3040..>3933
                     /locus_tag="VGB57_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY0131555.1"
                     /translation="MIERVSLASARRIALAAQGFGRPRPGAVAPGDLRRTVARLGLHQ
                     IDSVNVLVRAHYLPAFSRLGPYDRGLIDRAAWGPRRERRLFEYWAHEASLLPLEMHPL
                     LRWRMARAERGEIGWGSLKAYAGERRAEAEAILSRIRAEGPLAASDFEEGRGRSGWWE
                     WGDTKRALEWLFWAGLITTATRRGSFERVYDLAERVIPAAVLALPTPVEAEAKRALVA
                     KAAGALGIATETELRDYFRLKPGAARQAVAELVKEGVLRPVAVEGWIRPAFLHRDARR
                     PRRIRGQALLAPFDPLVWERER"
BASE COUNT          535 a         1321 c         1326 g          751 t
ORIGIN      
        1 cgggcctggc cgccgagccg cccttcgatg ccgacctcgc gggccatgtt ggtgacctgg
       61 tcgccgaaag tcgacaaagt ctcgatcatg aagttgatcg tctcggcaag cgcggcgatc
      121 tcgcccttgg cgtccacggt cagcttccgc ttgagattgc cctgcgccac ggcggtcacc
      181 acgtcggcga tgccgcgcac ctggttggtg aggttcgtcg ccatcaggtt gacgttgtcg
      241 gtcaaatcct tccacgtgcc gccgacgccc ggcacctgcg cctggccgcc caatttgccc
      301 tcggtgccga cctccctcgc gacgcgcgtc acctccgagg cgaagccgtt cagctgatcg
      361 accatgacgt tgatcgtgtt tttgagctcg aggatctcgc ccttcacgtc gaccgtgatc
      421 ttcttggaaa gatcgcccct cgccacggcg gtggtgacgt cggcgatgtt cctgacctgg
      481 ccggtcaggt tcgccgccat caggttcaca ttgtcggtga ggtcggccca ggtgccggcg
      541 atgccgcgca cctgcgcctg gccgcccagt ttgccttcgg tgccgacctc cctcgccacg
      601 cgggtcacct cggaggcgaa ggagttgagc tgatcgacca tcgtgttgat cgtgttcttc
      661 aattcgagaa tctcgccctt cacgtcgacg gtgatcttct tggaaaggtc acccagcgcc
      721 accgccgtcg tcacctcggc gatgttcctg acctggccgg tgaggttggc ggccatggcg
      781 ttcacattgt cggtgaggtc cttccaggtg ccgcccacgc ccggcacctg cgcctggccg
      841 ccgagcttgc cttcagtgcc gacctcgcgg gcgacgcgcg tcacctcgga ggcgaaggaa
      901 ttgagctgat cgaccatcac gttgatcgtg ttcttgagct ccaggatctc gcccttcacg
      961 tcgaccgtga tcttcttgga aaggtcgccc ttggccaccg cggtcgtcac ctcggcgatg
     1021 ttgcggacct ggccggtgag gttgccggcc atcaggttca ccgaatcggt aaggtcggcc
     1081 caggtgccgg cgacgccctc gacctgcgcc tggccgccga gcttgccttc cgagcccacc
     1141 tcgcgggcga cgcgggtcac ctccgaggcg aagccgttca gctggtccac catggtgttg
     1201 atggtgttct tgagctcgag gatctcgccc ttcacctcga cggtgatctt cttggacaag
     1261 tcgcccgagg ccacggcggt cgtcacctcg gcgatgttgc ggacctggcc cgtgaggttg
     1321 gtcgccatgg cattgacgtt gtcggtgagg tccttccagg tgccggcgac gcccttcaca
     1381 ttggcctggc cgccgagctt gccctcggtt ccgacctcgc gcgccacgcg ggtcacctcg
     1441 gaggcgaagg aggcgagctg ctccaccatg gtgttcacga tcttgccgat gcggaggaac
     1501 tcgcccctca gcggccggcc gtcgatctcg accgtcatcg actgcgaaag gtcgcccttg
     1561 gccaccgcgc cgatgactcg cgcgacctcg gcggtcggct ggaccatgtc ctcgatcagc
     1621 tcgttgaccg agcggatggc ggattcccag ccccccgtcg ccgccttgac ccggccgcgc
     1681 tggctgatct tgccttcctt gccgaccacc tgggagaggc ggccgagctc gtcggtcatc
     1741 tgctcgttca aggacacggt ctcgttgaac agccgcgcaa tctcgccgtc ctcgccggga
     1801 aggtcctcgt tgaggcggac ggtgaagtcg ccgcggcgga agctgcgcag ggcactgagg
     1861 agctggcggc gatcgatgaa atcgcgcggg gcctgtacgt tcaactcaac actccttcgt
     1921 gcgtcttccc cccgcccgat tttcagtgac aggcccccgc ccgttccggc aacatagccg
     1981 aaggatgaaa aaatccaccc cagaaatttg caaagcgtgc gattaaccag tcagccgggt
     2041 tgtcatggcg gcgagggcgg atagaaggcg gcgcgggcgg ggacaatttc tgacgcgcca
     2101 tggcggcgcg gcgaggatcg actgcctgca atgccctccg acgaggatgt ctccactttc
     2161 atccgctcca ccttccgttc ggtctgggca ctggaattgc tgtgcttcct caggcaggac
     2221 cgaagccgcg ccctctcgca cgcggagatg gttgcgggat tgagggggag cgatctggtg
     2281 gtcacgcaga gcgtggactc gctgacggcg gcggggctgg tggtcggcga gggcggcggc
     2341 tccgcccgct acaatccggc gtccggggag ctcgaccggc tggtcgagcg ggcggccgcg
     2401 ctctacgccc gaagtcccga cgcggtgcgg cgaacgatcg tcgccgccgc caatccgtcc
     2461 atcaccgcct tcgccgacgc cttcaagctc aggaaggatt gaggcgatgg cgacctggtt
     2521 cccggcttcg gtctatctgc tctgcttcat gaccagcgcg gcctgcgcaa ttctcctcac
     2581 gcgcagctat cgccgcaccg gcgcccgcct gctcttctgg agcgggctgt gcttcctgtt
     2641 cctcgccgcc aacaacctgg tggtgatatt cgacctgctt ctcctcccgg aggtcgattt
     2701 caggctggcc cgatacctgc tcgcgctggc ggcgctgggc gtgcttctgt tcggcttcat
     2761 ctgggacctg gaggactaga tggagacttt gttcgacttc ctctccgggg cgatcaccgc
     2821 gggcttcgcc gtcgccggct tcttcttcct gcgcttctgg aagcggaccc gtgaaagcct
     2881 gttcctcgcc ttcgcagcgg ctttctggct gctcggcctc ggccaggcgc ttctgacctt
     2941 caccgacatc ccggtcgagg agcgaagctg gctgttcctg ttgcggctgg ccgctttctc
     3001 gctgatcctg gtctcgatct ggaggaagaa ccggcgggcg tgatcgagcg cgtttcgctt
     3061 gcttcggcgc ggcgcatcgc actggcggcg cagggcttcg gccggccccg gcccggcgcg
     3121 gtggcacccg gcgacctgcg ccggacggtg gcccggctcg gcctccacca gatcgacagc
     3181 gtcaacgtgc tggtcagggc gcattacctt ccggctttct ccaggctcgg gccctacgat
     3241 cgcggcttga tcgaccgcgc cgcctggggg ccgaggcggg agcggcggct gttcgaatat
     3301 tgggcgcatg aagcctcgct cctgccgctg gagatgcatc cgctgctgcg ctggcggatg
     3361 gcgcgcgccg agcggggcga gatcggttgg ggcagcctca aggcttatgc cggcgagcgg
     3421 cgggccgagg cggaggccat cctctcccgg atccgggcgg aggggccgct cgcggcctcg
     3481 gatttcgagg agggcagggg ccggagcggc tggtgggaat ggggagatac caagcgcgcc
     3541 ttggaatggc tgttctgggc cggcctgatc accacggcca ctcggcgggg aagcttcgag
     3601 cgggtctacg accttgccga gcgggtgatc cccgccgcgg tcctggcgct gccgacgccg
     3661 gtggaggcgg aggcgaagcg ggcgctggtc gccaaagcgg cgggggccct cggaatcgcc
     3721 acggagacgg agctgcggga ctatttccgg ctgaagcccg gggccgccag gcaggccgtg
     3781 gcggagctgg tgaaggaggg agtcctgcgg ccggtggcgg tcgagggctg gattagaccc
     3841 gcttttctcc accgcgacgc ccgccgcccg cgccggatcc gcggccaggc tttgctggcg
     3901 ccgttcgacc ccctggtctg ggagcgcgaa cga
//
LOCUS       DASWNU010000466         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_96124, whole genome shotgun sequence.
ACCESSION   DASWNU010000466 DASWNU010000000
VERSION     DASWNU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96124"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VGB57_09130"
     CDS             <1..786
                     /locus_tag="VGB57_09130"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0131556.1"
                     /translation="VFGWSLPFEPDLLPPPIRDALEQSGTPSSEGALCRSRVRVASVE
                     DRLFLHSAYPTGAADSVFFGPDSYRYVRFLEAELPRLGPVRRLVDIGAGSGVGAIMAA
                     GMLPGASLTATDINPLALRFARINARHAGVELETVETSGLEAVDGPVDMVIANPPFVA
                     DPARRLYRDGGDMHGARLSLDWSLAAARRIEPGGTILLYTGSAIVDGRDGLEEALRRQ
                     LPALGCDVAYREIDPDIFGELLSEPGYEAVERIAAVGAVIRKA"
     gene            940..1155
                     /gene="rpsU"
                     /locus_tag="VGB57_09135"
     CDS             940..1155
                     /gene="rpsU"
                     /locus_tag="VGB57_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY0131557.1"
                     /translation="MQIIVRDNNVDQALRALKKKLQREGVYREMKLRRHYEKPSEKRA
                     RERAAAIRRARKLERKRAERDGGGAAR"
     gene            1241..1852
                     /locus_tag="VGB57_09140"
     CDS             1241..1852
                     /locus_tag="VGB57_09140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0131558.1"
                     /translation="MSVTAVPLRPVKKGSVAKLWIALALLAALAAAFAWWGTSAFQPV
                     RTATGVSIQTLAAGSGARITDQDVVALHYKLHARSLDAPVVEDSRAGGQPFVTTTQGV
                     YPGFGEGLQHMRPGGSYVLTLPPGTHVPGPIPPGAPFTAKDNLVFEIDVLQVEPGAGP
                     RYMEMQRMQQMQQMQQMQQMQGGAPGGPEGVPPPQGAPPEGAR"
     gene            complement(2027..3091)
                     /locus_tag="VGB57_09145"
     CDS             complement(2027..3091)
                     /locus_tag="VGB57_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0131559.1"
                     /translation="MRDPLIRHELKRASVWFGLALAIAAIIFLAQPLLLIFAGIVLAS
                     MLDGGTRLLGRVLPIGRGWRLAIVTITAVAFIVWVFYFAGTELTGQAERLREVITAQA
                     NRVLSWGNGLGIVQGGVGVEQIGGQLMGTLGRLGSAVSSALGVVASVVMVLVIGIFIA
                     VEPRLYQRGFAWMLPLGSRERFYSTAENMGFTLRRLMAGRLVGMAVEGIGTWILLALG
                     GVPMAALLGILTGLLAFIPNIGAIVSGVLIVLAGFSVSMNAGLWAIAVYFIVQTVDGY
                     LIVPYVARKTVDLAPALVLGAQLLFGALFGLMGLMLADPIVAMIKVALEQKSRDDEEE
                     DGDSAPGPAGQGPARLSPSP"
     gene            complement(3208..4065)
                     /gene="lepB"
                     /locus_tag="VGB57_09150"
     CDS             complement(3208..4065)
                     /gene="lepB"
                     /locus_tag="VGB57_09150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0131560.1"
                     /translation="MPPSEAAPEAEAKKEGTDWWGEIKAIFWLILAVLAFHSFIAKPF
                     YIPSESMMPGLLKGDRLVVSKYPYGWSWVSPSFHVLPPMGGRLFGRMPERGDIVILTP
                     PGKTSDYIKRVIGLPGDTLEMQGGILYINGREVKRERRAPVMVEVDANVPCELDQYGE
                     HRLQGADGKLHCRLPVFRETLPNGRSYDTIDMGLSDVDNFARVVVPPGHVWVQGDNRD
                     KSADSRVSLAGDGLGGAVPWENIGGRAEFITFSLDGSSQYLNPISWITAMRGGRAGTS
                     LRPEEGKAP"
BASE COUNT          675 a         1382 c         1415 g          603 t
ORIGIN      
        1 gtcttcggct ggagccttcc gttcgagccc gacctgctgc cgccgccgat aagggacgcg
       61 ctcgagcaaa gcggaacccc gtcgagcgag ggggcgctgt gcaggagccg ggtgcgggtc
      121 gcttcggtgg aggaccgatt gttcctccac tccgcttacc cgacaggggc agccgattcc
      181 gtcttcttcg gccccgacag ctaccgctac gtccgcttcc tggaggccga gctgccccgc
      241 ctcggcccgg ttcgccggct cgtcgacatc ggcgcgggca gcggagtcgg ggcgatcatg
      301 gcggcaggga tgttgcccgg cgcctcgctc accgccaccg acatcaaccc gctcgccttg
      361 cgcttcgccc gaatcaacgc ccgccacgcc ggcgtggagc tcgaaacggt cgagacgagc
      421 ggcctcgagg cggtcgacgg accggtggac atggtcatcg ccaacccgcc tttcgtcgcc
      481 gatccggcca ggcgcctcta tcgcgacggc ggcgacatgc acggcgcccg cctctcgctc
      541 gactggtcac tcgccgccgc ccgccggatc gagcccgggg gaacgatcct cctttatacg
      601 ggaagcgcga tcgtggacgg gcgcgacggt ctggaggagg cgctgcgccg gcagctgccg
      661 gcgctcggct gcgacgtggc atatcgggaa atcgatccgg acattttcgg cgagctgctg
      721 agcgaaccgg gctatgaggc ggtcgagcgg atcgccgcgg tcggagccgt catccgcaag
      781 gcgtgacttg aatcccgccg ccgcccctgc taacggcccc ggcgtgacgg cgcgggagga
      841 gtctcgcgac gaagcgattt ttatgctatc ggcgtttccg caatgggcga cctgagaggt
      901 tgccgggaaa tgcttttcga cggagagtga cgggccttta tgcaaatcat cgttcgcgac
      961 aacaatgtcg accaggcgct gcgggcgctc aagaagaagc tgcagcgtga gggcgtctat
     1021 cgcgagatga agcttcgccg tcactacgag aagccgtcgg agaagcgcgc ccgcgagcgc
     1081 gccgccgcga tccgccgcgc ccgcaagctc gagcgcaagc gcgccgagcg cgacggcggc
     1141 ggggccgccc gctagacgcg gcgactcggc cgggaaaccg gcgcttgctt cacgcgaggg
     1201 cggcgatgta tcgccgccct tcgcatttca ggggatgatc atgtccgtca cagccgtacc
     1261 gcttcgtccc gtgaagaagg gctcggtcgc caagctctgg atcgccctcg ccctgctcgc
     1321 cgccctcgcc gcggctttcg cctggtgggg cacgagcgcc ttccagccgg tgaggaccgc
     1381 gaccggagtc tcgatccaga cgctcgcggc cgggtccggc gccaggatca ccgaccagga
     1441 cgtcgtcgcg ctccactaca aattgcacgc caggagcctc gacgctccgg tcgtcgagga
     1501 cagccgggcg ggcggccagc ccttcgtcac caccacccag ggcgtctatc ccggtttcgg
     1561 cgagggcctc cagcacatgc gccccggcgg ctcctacgtc ctcacccttc cgcccgggac
     1621 ccacgtcccc gggccgatcc cgccgggcgc gcccttcacg gcgaaggaca atctcgtctt
     1681 cgagatcgac gtcctccagg tcgagccggg cgccggcccg cgctacatgg agatgcagcg
     1741 catgcagcag atgcagcaga tgcagcagat gcagcagatg cagggcggcg ccccgggcgg
     1801 accggagggc gtccccccgc cgcagggcgc accgccggag ggcgctcgct gaactaaatt
     1861 cctccccgcg atttcgcggg gagggggacc gcgcgaagcg cggtggaggg gttctttgga
     1921 gtcagcaagg cccctccacc aggcttcgcc tggtccccct cccctgcaaa tgcaggggag
     1981 gaatttatag gtcgtgcccg gccgcgagct ggaccctgaa acgagttcag ggtgacgggg
     2041 agaggcgcgc aggaccctgg cccgccggac ccggtgccga gtcgccatcc tcttcctcgt
     2101 cgtcgcggga cttttgttcg agggcgacct tgatcatggc gacgatggga tcggcgagca
     2161 tcaggcccat cagtccgaac agggcgccga acaggagctg ggcgcccagc acgagggcgg
     2221 gcgcgaggtc gacggtcttg cgggcgacgt aggggacgat cagatagccg tcgacggtct
     2281 ggacgatgaa gtagacggcg atcgcccaaa ggcccgcatt catgctgacg ctgaagccgg
     2341 cgaggacgat gaggacgccc gagacgatcg ctccgatgtt ggggatgaag gcgagcaggc
     2401 cggtgaggat tccgagcagg gccgccatcg gcactccgcc cagggcgagc aggatccagg
     2461 tgccgatccc ctcgaccgcc atcccgacca gacgcccggc catcagccgc cgaagggtga
     2521 agcccatatt ctccgcggtg ctgtagaagc gctcgcggct gccgagcgga agcatccagg
     2581 cgaacccgcg ctggtagagg cggggctcga cggcgatgaa gatgccgatg acgaggacca
     2641 tcacgaccga ggcgacgact cccagcgcgc tcgacaccgc ggagccgagc cggcccaggg
     2701 tgcccatcag ctgcccgccg atttgctcga cgcccactcc gccctggacg atcccgaggc
     2761 cgttgcccca ggagagcacg cggttggcct gagcggtgat gacctcgcga agccgttcgg
     2821 cctggccggt caactcggtg ccggcgaaat agaagaccca gacgatgaag gcgacggccg
     2881 tgatcgtcac gatggcgagg cgccagccgc ggccgatcgg aagcacccgg ccgagcagcc
     2941 gggtgccgcc gtcgagcatc gaggcaagga cgattccggc gaagatcaga agcaggggct
     3001 gggcgaggaa gatgatcgcc gcgatggcga gggcgagtcc gaaccagacg cttgcgcgct
     3061 tcagctcatg gcggatgagc gggtcgcgca tctccgcggg gccgggctgc tcgacgtggc
     3121 gttcatgatg atcgggatcg gacgaagccc gggcgatcgc tggagcgtcg gagtgccggc
     3181 ccggcgagga tggcgccccc tcctcgctca cggcgccttg ccctcctccg gccggagcga
     3241 ggtgccggcg cggccgccgc gcatcgccgt gatccagctg atcgggttca gatattgcga
     3301 ggatccgtcg agggagaagg tgatgaactc ggcccggccg ccgatattct cccagggcac
     3361 ggcgccgccc agcccgtcgc ccgccagcga cacccggctg tccgccgact tgtcgcgatt
     3421 gtcgccctgc acccagacat ggccgggagg aacgacgacc ctggcgaaat tgtcgacgtc
     3481 ggacaggccc atgtcgatcg tgtcgtagct gcgcccgttc ggcagggtct cgcggaagac
     3541 gggaagccgg caatgaagct tgccgtcggc accctgaagc ctgtgctcgc catattggtc
     3601 aagctcgcag ggcacgttgg cgtccacctc gaccatcacg ggagcccgcc gctcgcgctt
     3661 cacctcgcgg ccgttgatgt agagaatgcc cccctgcatc tcgagcgtgt cgccgggaag
     3721 tccgatgacc cgcttgatat agtccgacgt cttgcccggc ggggtgagga tgacgatgtc
     3781 gccgcgctcc ggcatccgcc cgaacaggcg tccgcccatc ggcggcagga cgtggaagct
     3841 gggcgagacc caggaccagc catagggata tttgctgacg acgagccggt cgcccttgag
     3901 aagcccgggc atcatcgatt ccgacgggat gtagaagggc ttggcgatga agctgtggaa
     3961 ggcgagcacg gccaggatca gccagaagat cgccttgatc tcgccccacc aatccgtgcc
     4021 ctccttcttc gcttccgctt cgggcgcggc ttcggaaggg ggaatgtcgt cggtc
//
LOCUS       DASWNU010000467         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_172954, whole genome shotgun sequence.
ACCESSION   DASWNU010000467 DASWNU010000000
VERSION     DASWNU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172954"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGB57_09155"
     CDS             complement(<1..375)
                     /locus_tag="VGB57_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0131561.1"
                     /translation="MSSDRPQQNRLAGIGLRVAAATCFAFMAMLVKLGHEAGVGTVEM
                     AFYRFAFGLPPLLLWIAASRNFGAWRTRRPLAHVWRAAAGLTTLILSFSALALLPLAE
                     STTIGFAAPLFSVALSALILGES"
     gene            495..1208
                     /gene="ccmC"
                     /locus_tag="VGB57_09160"
     CDS             495..1208
                     /gene="ccmC"
                     /locus_tag="VGB57_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEY0131562.1"
                     /translation="MHILANPTRFLRLARPLTGWLGWSGALLIALGLGIGLFVAPPDY
                     LQGDTVRIMYVHVPAAWLGMAGWAGIAVASLMQIVWRHPLAAVAARALALPGAVFTFI
                     CLATGSLWGRPAWGTWWEWDGRMTSMLVLLFLYVGFIALADAERERGGEGRLAAIFGL
                     VGAINLPVIHYSVIWWRTLHQGQSIGLGRSSIDSSMLLPLFVSAAGFSLLFGAIVLMR
                     MRAALARQKVEARMRRMAA"
     gene            1205..1336
                     /locus_tag="VGB57_09165"
     CDS             1205..1336
                     /locus_tag="VGB57_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY0131563.1"
                     /translation="MSHWPFIFGAYAVALLGTLGLTGWSLAAMRRAERAVDALKGER"
     gene            1333..1773
                     /gene="ccmE"
                     /locus_tag="VGB57_09170"
     CDS             1333..1773
                     /gene="ccmE"
                     /locus_tag="VGB57_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY0131564.1"
                     /translation="MKAKNQRLVLLVLAIAAVLGAVLLAMSALKDQAAYFYAPGDVAR
                     EGPPVGRAVRIGGMVQAGSLTRAPDGVTIRFTVKDETPHTIPVRFTGIVPDLFKENSG
                     VVAEGRFQAGGLFVADNILAKHDENYMPPQLDPKKGEHKSDSLK"
BASE COUNT          332 a          728 c          730 g          366 t
ORIGIN      
        1 cgactcgccg aggatcaggg ccgaaagcgc gaccgagaag agcggcgccg cgaagccgat
       61 cgtcgtcgac tccgccagcg gcagcagagc gagcgcgctg aagctgagga tcagggtcgt
      121 cagccccgcc gccgcccgcc agacatgcgc cagaggccgc cgggtccgcc aggcgccgaa
      181 attgcggctc gccgcgatcc acagcagcag cggcggcagc ccgaaggcga agcggtagaa
      241 ggccatctcg accgttccca ccccggcctc gtggccgagc ttcaccagca tcgccatgaa
      301 ggcgaagcag gtggcggccg ccactcgcag gccgatgccg gcgagccggt tctgctgggg
      361 acggtcggag gacatggact cgtcattgcg aggagcggag cgacgaagca atccagcgcg
      421 gcttcagacg cgcaccggat tgcttcgctt cactcgcaat gacgggtcgc ccacgctaca
      481 tacgccccgg gcaaatgcat attctcgcga atccgacacg cttcctgcgg ctggcgcggc
      541 cgcttaccgg ctggctcggc tggagcgggg cgttgctcat tgccttgggg ctcggcatcg
      601 gcctgttcgt agcaccgccc gattacctcc agggcgacac cgtccggatc atgtacgtcc
      661 atgttccggc cgcctggctc ggaatggccg gctgggcggg aatcgccgtg gcgagcctga
      721 tgcagatcgt ctggcgccac ccgcttgccg ccgttgccgc gcgggcgctt gccttgccgg
      781 gcgcggtctt taccttcatc tgcctcgcca ccggctcgct ttggggaagg cccgcctggg
      841 gcacctggtg ggaatgggac gggcggatga cgtcgatgct cgtcctcctc ttcctctatg
      901 tcggcttcat cgctctcgcc gacgccgagc gcgagcgggg cggggaggga cgtctcgccg
      961 ccattttcgg cctggtcgga gcgatcaacc tgccggtcat ccattattcg gtgatatggt
     1021 ggcgcactct ccaccagggg cagagcatcg ggctcgggcg ctcgagcatc gatagctcga
     1081 tgctgttgcc cttgttcgtt tcggccgcgg gcttctccct tcttttcggg gcgatcgtgc
     1141 tgatgcggat gcgggccgcg ctcgcccggc agaaggtcga ggccaggatg cgcaggatgg
     1201 cggcatgagc cactggccgt tcatcttcgg cgcctacgcc gtcgccctcc tcggaacgct
     1261 cggcctgacc gggtggagcc tcgcagcgat gcgccgagcc gagcgcgccg tcgacgcctt
     1321 gaagggcgag cgatgaaggc gaagaaccag cgcctggtcc tgctcgtcct cgccatcgcc
     1381 gccgtgctcg gcgccgtgct gctcgccatg tcggcgttga aggatcaggc cgcctatttc
     1441 tacgcccccg gcgacgtcgc gcgcgaagga cctcccgtcg gccgcgccgt ccgcatcggc
     1501 ggaatggttc aggccggctc tctgactcgt gcgcccgatg gggtcacgat ccgcttcacg
     1561 gtcaaggacg agactccgca caccatcccc gtgcgcttca ccggcatcgt ccccgacctg
     1621 ttcaaggaga atagcggagt cgtcgccgag ggccgctttc aggccggggg cctgttcgtc
     1681 gccgacaata tcctcgccaa gcatgacgaa aattacatgc cgccccagct cgacccgaaa
     1741 aagggcgagc ataagagcga cagcctcaaa tgatcgccga gctgggactg gcggcgttgt
     1801 ggctcgcggc ggcgctgtcg ctgctgcaat tgggcatggg gattgcctct ctttgcctcc
     1861 ccgcgagcga agctcggggg gagggggacc atgcgcggca tggtggaggg ggcctgttgc
     1921 agcgcgggtg tgcctgaaga ctcgccccct cagtcggctt cgccgacagc tcccccaaag
     1981 gggagcatcc aggagcaggt ggttgcggcg caaggtgcgt ccttcgagac gcccttcggg
     2041 ctcctcagga tgacgagtag gtgtggcgcg caaacttttc atcatggtga gggcgggcga
     2101 agcccgtgtc tcgaaccacg cacgccgtcg atccagccag accttcctcc cccaaa
//
LOCUS       DASWNU010000468         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_355552, whole genome shotgun sequence.
ACCESSION   DASWNU010000468 DASWNU010000000
VERSION     DASWNU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355552"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="VGB57_09175"
     CDS             <1..1739
                     /locus_tag="VGB57_09175"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0131565.1"
                     /translation="KRSPLDRRVLAEALAALDGMRPKSIGIDILLDQPQAEDPQLLAT
                     LRNMRTPTSIAYADPAGNEDQIGYEQHQFLRAFLAEARTDRVRPASIRMEPDVEDGVI
                     RRWPERRGNLPPLLANAMTAAHPEFGHHSGSIDFLLPADPERPVFASFAIDTLPLLGD
                     AIRDKIEGRHVLIGGNIKDQDDYETPMSRRTGRWMKGVEVHAHMLAQQLDGRLRAPVR
                     PAVLWAIAVALVLAGGLTSLVELGGWRMALALAVQLALIAALPFALQQWGMDTYAVPA
                     FGWGLGWAFAFAAAGTAARAVGSEQRRFAQAALGKYLPADVAAEILKDPERLTLSGEK
                     RQIYALFSDLEGFTKLSHAISPEQLSGLLNRYLEMLSGVVLSHGGTIDKFVGDAIVAF
                     WGAPIAREDDADRAALAAVALYEAGEAFRRSAGADMPAIGCTRVGLHHGEAVVGNFGG
                     EGRIQYTALGDAMNTASRLESANKQLHSTILVSAEAKGRTSLDIFRPLGRVVLNGRAT
                     PVEVWEPVPHMPAQERARLDGLWRAFDSGDAEALETLAAIASGGEDAALADFVYRLRQ
                     AGPGGHFVLGSK"
     gene            1720..2424
                     /locus_tag="VGB57_09180"
     CDS             1720..2424
                     /locus_tag="VGB57_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131566.1"
                     /translation="MSWGPSKAFARPLLAGAFLLTASAALADILVVRSTGPSARSFAP
                     GKRLPETARISLRPGDQLIVLDGRGTRVIRGPGTFTAGVPTVARLDSGPASQRRARIG
                     AVRGVETGELRPPSIWHVDVAKSSNVCVADPAKVTLWRADPGKGATLTIAGQAGRTKQ
                     LGWQPGSSTLAWPSDLPVAEGVEYRLSGGNAAPSTIRFRTMPAAPAGLEDMAQTLIRN
                     KCEAQLDLFIDTVKLP"
     gene            complement(2564..>3220)
                     /locus_tag="VGB57_09185"
     CDS             complement(2564..>3220)
                     /locus_tag="VGB57_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY0131567.1"
                     /translation="LDDAETLFHEFGHAIHYFISNVYYPSFGGTPRDFVEYPSQVNEN
                     WLLTQPVLSRFARHYQTKQPMPASLIQKIEKASTFNQGFATAEYLSSAIVDMMLHMDP
                     NGVVDPNAFEAQALAKIGMPKELVMRHRLPQFNHLFSSDAYSAGYYSYLWSETMDADT
                     WAAFEEAGNPFDKATADRFRRTLLSTGNETDRAEAYRAFRGRDPDVKALMRKRGFPTG
                     "
BASE COUNT          515 a         1103 c         1080 g          522 t
ORIGIN      
        1 agaagcgctc gccgctcgac cgccgggtcc tcgccgaagc cctagcagcg ctggacggga
       61 tgcggcccaa gtcgatcggg atcgatatcc tgctggatca gccgcaggcc gaggatccgc
      121 agctcctcgc caccctcagg aacatgcgga cgcccacttc gatcgcttat gcggacccgg
      181 ccggaaacga ggatcagatc ggctacgagc agcatcaatt cctgcgcgcc ttcctcgccg
      241 aggcgcggac cgaccgggtt cggccggcca gcatcaggat ggagcccgac gtcgaggacg
      301 gagtcattcg ccgctggccc gagcgcaggg gcaatctccc gcccctgctc gccaatgcca
      361 tgaccgcggc ccacccggaa ttcgggcatc attcggggag catcgacttc ctgctgcccg
      421 ccgatccgga gcgtcccgtc ttcgccagct tcgcgatcga cactttaccg ctgctcggcg
      481 acgcgatccg cgacaagatc gagggccgac acgtcctgat cggcggcaac atcaaggacc
      541 aggacgatta cgagactccg atgagccgcc ggaccggccg ctggatgaag ggcgtggagg
      601 tccacgccca catgctcgcc cagcagctcg acggtcggct ccgcgccccg gtgcggccgg
      661 cggtcctgtg ggcgatcgcc gtcgccctgg tgctcgccgg cgggctgacc agcctcgtcg
      721 agcttggcgg ctggcggatg gcgctggcgc tggcggtgca gctcgcgctc atcgccgcct
      781 tgcccttcgc cctgcagcaa tggggcatgg acacttacgc cgtgcccgct ttcggctggg
      841 ggctgggctg ggccttcgct ttcgccgccg cggggacggc ggcgcgcgcc gtggggtccg
      901 agcagcgccg gttcgcgcag gcggctttgg gcaaatatct tccggccgac gtcgccgccg
      961 agatattgaa ggacccggag cggctgaccc tgagcggcga aaaaaggcag atctacgcgc
     1021 tcttctccga tctcgagggc ttcacgaagc tcagccatgc aatcagcccg gagcaattgt
     1081 cgggccttct caaccgctat ctcgaaatgc tgagcggcgt cgtcctgagc catggcggaa
     1141 ccatcgacaa gttcgtcggc gacgccatcg tcgccttctg gggcgcgccg atcgcgaggg
     1201 aagacgatgc cgaccgggcc gccctagccg ccgtcgcttt gtatgaggcg ggagaggcgt
     1261 tccggcgcag cgccggcgcg gacatgcccg ccatcggctg cacccgcgtc ggacttcatc
     1321 atggcgaggc ggtagtcggc aatttcggcg gcgagggccg gatccagtat accgcgctcg
     1381 gcgacgccat gaacacggcg tcgcggctgg aatcggcaaa caagcagctc cactccacga
     1441 tcctggtcag cgccgaggcg aaggggcgga cgagcctcga catcttccgt ccgctcggcc
     1501 gggtcgtcct caacggccgg gcgactccgg tcgaggtctg ggagccggtt ccgcacatgc
     1561 cggcgcagga gagagcccgg ctcgacgggc tctggcgggc gttcgattca ggcgatgccg
     1621 aagcgctgga aaccctggcg gcgatcgcct ccgggggaga ggatgccgct ctcgccgatt
     1681 tcgtgtatcg tctccgccag gcaggtccgg gaggtcattt tgtcttgggg tccaagtaaa
     1741 gcgttcgcgc gcccgctgct cgcgggcgcc ttcctcctca ccgccagcgc cgccctggcc
     1801 gacatcctcg tcgtccgctc caccggcccg tccgcaagga gcttcgcgcc tggcaagcgc
     1861 cttccggaaa ccgcacggat cagcctcagg cccggcgacc agctgatcgt cctggacggc
     1921 cgcggcaccc gggtcattcg cggccccggc actttcaccg ccggcgttcc caccgtcgcc
     1981 cgactcgact caggacccgc cagccagcgc cgcgcccgaa tcggcgccgt ccggggagtg
     2041 gagacgggcg agcttcggcc gccgagcatc tggcatgtgg acgtcgcgaa gagctccaac
     2101 gtctgcgtcg ccgatccggc caaggtgacc ctgtggcggg ccgatcccgg caaaggtgcg
     2161 actttgacta tcgccggcca ggcaggccgg acgaagcagc taggctggca gcccggctcc
     2221 agcaccctgg cctggccatc cgatctcccg gtggccgaag gggtcgaata ccgcctctcc
     2281 ggcggcaatg cggcgccgtc gacgatccgc ttccgaacca tgcccgccgc gcccgccggc
     2341 ctcgaggaca tggcccagac ccttatccgc aataagtgcg aagctcagct cgacctcttc
     2401 attgacacgg tgaagctgcc gtaaccttgc ctccccatgc gaagcatggg gagggggacc
     2461 accggaggtg gtggaggggt catctgacgc ggcttgcgcc agctacccct ccaccccgct
     2521 acgcggggtc cccctcccca tctggcgatg gggaggcaag atactacccg gtcgggaaac
     2581 cccgcttgcg catgagggcc ttcacgtcgg gatcgcggcc gcggaaggcg cggtaggctt
     2641 cggcgcggtc ggtctcgttg ccggtcgaga gcagggtccg gcggaagcgg tccgccgtcg
     2701 ccttgtcgaa cggattgcca gcctcttcga acgccgccca ggtgtcggcg tccatcgtct
     2761 ccgaccagag gtaggaataa tagccggccg agtaagcgtc ggacgagaag aggtggttga
     2821 actgcggcag acggtgccgc atcaccagct ccttcggcat tccgatcttc gccagcgcct
     2881 gggcctcgaa cgcgttcgga tcgacgaccc cgttcgggtc catgtggagc atcatgtcga
     2941 cgatcgccga cgacagatat tccgccgtgg cgaagccctg gttgaaggtc gaggccttct
     3001 cgatcttctg gatcagcgat gccggcatcg gctgcttcgt ctggtagtgc ctggcgaagc
     3061 ggctgagcac cggctgggtc agaagccaat tctcgttcac ctggctcgga tattcgacga
     3121 agtcgcgggg agtcccgccg aagctcggat aatagacgtt cgagatgaag tagtggatgg
     3181 cgtggccgaa ttcgtggaaa agggtctcgg catcgtcgag
//
LOCUS       DASWNU010000469         5204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_458834, whole genome shotgun sequence.
ACCESSION   DASWNU010000469 DASWNU010000000
VERSION     DASWNU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458834"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..861
                     /locus_tag="VGB57_09190"
     CDS             325..861
                     /locus_tag="VGB57_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY0131568.1"
                     /translation="MAAKALSFDVGDYVVYPKHGVGRVIELQSTDIAGMQLELYVLRF
                     EKEKMTLRVPTNKAESVGMRKLSSDKTMQEALATLKGKPKVKRTMWSRRAQEYEAKIN
                     SGDLVSIAEVTRDLFRADDQPEQSYSERQIFEAASSRLARELAAMEQVDEPAALVKIL
                     EILNKAAAVYNREKAAAV"
     gene            1040..1753
                     /locus_tag="VGB57_09195"
     CDS             1040..1753
                     /locus_tag="VGB57_09195"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY0131569.1"
                     /translation="MRGAILIPVLMLGACSMGEASESKGQAGSRAFQVGAFDKISLQG
                     SPNVVVTVGGAPSVRAEGDGKLIEKLEVTVENGTLRIGYKKGDWSFGWGKDHAPVTVH
                     VSVPSLTGAELAGSGDMKIDKVEGGDFAGEIAGSGEIELASLRARNASFAIAGSGSVT
                     ANGTAETADFSIAGSGDVRAAGLQVKRARVSIAGSGNVETKATESANVDIMGSGDVLV
                     TGGARCSVNKMGPGDVRCG"
     gene            1869..2570
                     /locus_tag="VGB57_09200"
     CDS             1869..2570
                     /locus_tag="VGB57_09200"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY0131570.1"
                     /translation="MIRILPLALLAFAAAVPAGAAERSYSITDFDRVQVDGPYRVTLT
                     TGRSSAARAEGSTEALDHVSVDVQGGTLRVRRNRSAWGGYPGGGAGPVTIALTTRDLR
                     NAAVVGSGSLDIDRARGLRVDLSVSGSGRLTVGSVEADNLIVGLLGGGRITVAGRARQ
                     LKATLQGSGDFAASGLSTDDAQIASDTAGSVAVAVTRTARVNATGSGDVEIIGSPACT
                     VQARGSGQVLCGSAR"
     gene            2603..4150
                     /locus_tag="VGB57_09205"
     CDS             2603..4150
                     /locus_tag="VGB57_09205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0131571.1"
                     /translation="MTARSIAFPLLGSWAVSAGGLRPGPLLLLLALPVLAALPSGYQG
                     GGGDDWHYLQAARCWNASGGWLTGGACLPTDHWQVRWPLIAPMALAIGWLGESRASVQ
                     LVPLLYALGALLLFSSLVERLFGRGAALAGGAVLLFTPAFAMTMVQPTINIVEIAFLL
                     GFGAAALTALESGRARWAFAAGLSLALAIASRETAVMTGLVAAGAGLFLPAARRRLGI
                     AAAAGAAVPILLEILLFAVRTGDPLRRAALALGHVAIPSKEFVGKAGPGGLPLFNPEV
                     IGGWVPACGIDLHWAVNGPVNLIVHPHYGLTLLATFVLAIACGRSSLSDPQTRRRLVH
                     LAWAGGLIAFLLIYGLAIDPKPRMFLLPLCAVAAICGALASRAWRDGRRALVLAFALL
                     LPAKAILFLLSGPDFAPAERLAAQWLAARPGEVAIPETTRRNLALVPAAAAARSDDAA
                     PYVLDIRWSACSEAPLGAVVEEYGFGASEPRFLPPASPGLTMCLYRRAPRAGWVQFES
                     VGDSHGG"
     gene            4194..5142
                     /locus_tag="VGB57_09210"
                     /pseudo
     CDS             4194..5142
                     /locus_tag="VGB57_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          798 a         1755 c         1808 g          843 t
ORIGIN      
        1 ggtggctggg gcggcgcggc cgctctcccg tccgtcatcc cggcgaaagc cgggatctcg
       61 gtgagacccg ccgtgacttt agcggcctga ggtcccggct ttcgccggga tgacggtctt
      121 gtgtcgcatc cattaccctg cgcggtcaaa agggtagctt ttccgcacct tttcggttat
      181 aaggtgtcca tcgaagcgga tgcgacgcgc attccgcaac cggtggtcac aagaagaatc
      241 gccgcacatc gcagtttggg tacgtccctg gggcggatgg agtccggcgc ggatgcggct
      301 gttttccaat ggaaggtcga ataaatggca gcgaaagcgt tgagcttcga cgttggcgat
      361 tacgttgttt accccaagca cggcgtgggc cgggttatcg aactgcagag cacggacatc
      421 gccggaatgc agctcgagct ctacgtgctt cggttcgaga aggaaaagat gaccctgcgc
      481 gttccgacca acaaggcgga aagcgtcggc atgcgcaaat tgtcgtccga caagacgatg
      541 caggaggcgc tcgccaccct caagggcaag cccaaggtga agcgcaccat gtggtcgcgc
      601 cgcgcccagg aatatgaagc gaagatcaac tcgggcgacc tcgtgtcgat cgccgaggtg
      661 acccgcgatc tgttccgcgc cgacgaccag cccgagcaga gctattcgga gcggcagatc
      721 ttcgaggccg cctcgagccg cctcgcccgc gagctggccg cgatggagca ggtcgacgag
      781 ccggccgcgc tggtgaagat cctcgagatc ctcaacaagg ccgcggccgt ctacaaccgg
      841 gagaaagcgg cggcggttta gccgtcgccc cagcgaaggc tggggcccca gtgggtgaag
      901 ggcgcggcgc ttttcacacc ctgggtcccg gcctccgccg ggatgacgaa ggcggtccgg
      961 cgacgggccg cctgttttgt tgcgcacgca ttccgccacg tgtattagaa acataataca
     1021 gcgtgaggag gagatggaga tgcgcggtgc gattctgatt ccggtgctga tgctcggcgc
     1081 ctgctcgatg ggcgaggcca gcgagagcaa gggccaggcg ggaagccgcg ctttccaggt
     1141 cggcgccttc gacaagattt cgctccaggg gtcgccgaac gtcgtcgtga cggtcggcgg
     1201 cgccccgtcg gtccgcgctg agggcgacgg caagctcatc gagaagctcg aggtgacggt
     1261 cgagaacggg accctccgga tcggctacaa gaagggcgat tggtcgttcg gctggggcaa
     1321 ggatcacgcc ccggtcaccg tccacgtctc ggtgccctcg ctgaccggcg cggaattggc
     1381 tggctcgggc gacatgaaga tcgacaaggt cgagggcggc gacttcgccg gcgagatcgc
     1441 gggatcgggc gagatcgagc tcgccagcct gcgcgcccgc aacgccagct tcgccatcgc
     1501 cggttccggc agcgtgacgg cgaacgggac ggccgagacc gcggacttct cgatcgccgg
     1561 ctcgggcgac gtccgcgccg ccggcctcca ggtgaagcgg gccagggtgt cgatcgccgg
     1621 ctcgggcaat gtcgagacca aggcgaccga gagcgccaat gtcgacatca tgggctcggg
     1681 cgacgtgctc gtcaccggcg gcgcccggtg cagcgtcaac aagatgggcc cgggcgacgt
     1741 gcgctgcggg tagaccgtat cccggcgaag gccgggaccc agtcttaccg gcgccgccgc
     1801 tgggccccgg ccttcgccgg ggaacgggcg gttatcggcc gttaaccatt ttccggcagg
     1861 gtccggccat gatcaggatc ctgccgctcg ccctcctcgc tttcgccgcc gccgtcccgg
     1921 cgggcgccgc cgagcgaagc tattccatca ccgacttcga ccgggtgcag gtcgacgggc
     1981 cctatcgggt caccctgacc accggacgaa gctccgccgc ccgggccgag ggatcgaccg
     2041 aggcgctgga ccatgtctcg gtggacgtcc agggcggcac cctcagggtc aggcgcaacc
     2101 gttcggcctg gggcggctat cccggcggcg gggccgggcc ggtcacgatc gccctgacca
     2161 cccgcgacct gcgcaacgcc gcagtggtcg gatcgggcag cctcgacatc gaccgggcga
     2221 ggggccttcg ggtcgacctc tccgtgtccg gaagcgggcg gctgacggtg ggctcggtcg
     2281 aggcggacaa cctcatcgtc ggactgctcg gcgggggccg aatcacagtc gccgggcgcg
     2341 ccaggcagct caaggcgact ctccagggca gcggcgactt cgccgcctcc ggattgagca
     2401 cggacgacgc ccagatcgcg tccgacacgg ccggaagcgt cgccgtggcc gtgacccgga
     2461 ccgccagggt gaatgcgacc ggatccggcg acgtcgagat catcggaagc ccggcctgca
     2521 ccgtgcaggc cagggggtcc ggccaggtac tctgcggcag cgcccgctag cctgacctgc
     2581 gccgaatgag aactcagcgg aagtgacggc gcggtcgatc gcctttccat tgctcggttc
     2641 ctgggccgtg tccgcgggcg ggcttcgtcc gggccctttg ctgctcctgc tcgccctccc
     2701 ggtgctcgcg gcgcttccca gcggctatca gggcggcggc ggcgacgatt ggcattacct
     2761 ccaggcggcg cgctgctgga acgcttccgg tggctggctg accggcggcg cctgccttcc
     2821 gaccgatcac tggcaggtgc gctggccgct gatcgcgccg atggcgctgg cgataggctg
     2881 gcttggcgaa agccgggcca gcgtccagct ggtgcccctc ctctacgcct tgggcgcact
     2941 gctcctcttt tcgtcattgg tcgaacggct gttcggccgc ggcgccgctt tggccggcgg
     3001 tgcggtcctg ctgttcacgc ccgccttcgc gatgaccatg gtgcagccca cgatcaacat
     3061 cgtcgagatc gccttcctgc tcggattcgg ggccgctgcc ctcaccgcgc tggaaagcgg
     3121 ccgcgcgcga tgggcgttcg cggcgggcct ctcgctcgcc ctcgccatcg cctctcgcga
     3181 gaccgccgtg atgacgggcc tggtcgcggc cggagcgggg ctgttccttc cggcggcaag
     3241 gcgaaggctc ggaatcgcgg ccgcggccgg agccgccgtt ccgatcctgc tcgagattct
     3301 tctcttcgcg gttcgcaccg gcgatccgct gaggcgcgcc gccctcgctt tgggccacgt
     3361 cgcgatcccg tcgaaggaat tcgtcggcaa ggccgggccc ggcggtctgc cgctgttcaa
     3421 tccggaggtc atcggcggct gggtcccggc gtgcgggatc gacctccatt gggccgtgaa
     3481 cgggccggtc aacctcatcg tccatcccca ttacggcctc accctgctgg ccacctttgt
     3541 gcttgcgata gcctgcggcc gatcgagcct gagcgacccg cagacccggc gccgcctcgt
     3601 ccatctggcc tgggccggcg ggctgatcgc cttccttctc atctacgggc tcgccatcga
     3661 cccgaagcct cgaatgttcc tgcttccgct ctgcgcggtg gcggcgatct gcggagcgct
     3721 cgcctcccgc gcgtggaggg acggacggcg cgcgctggtg ctggccttcg ccttgctgtt
     3781 gccggcgaag gcgattctct tcctcctctc gggccctgat ttcgcgcccg ccgagcggct
     3841 cgcggcgcaa tggctcgccg cccgtccggg cgaggtcgcg attcccgaga cgacccggcg
     3901 caacctggcg ctggtcccgg ccgccgccgc cgcccgcagc gacgatgccg cgccctatgt
     3961 cctcgatatt cgctggagcg cctgcagcga ggcgcctttg ggagcggtgg tcgaggaata
     4021 cggtttcggc gccagcgaac cgcgcttcct gcctccggcc agccccgggc tgaccatgtg
     4081 cctctaccgc cgggccccta gagcgggctg ggttcagttt gaatcggtgg gggattccca
     4141 cggcggctaa gttgtgattc aagctgaggg ctggcgcgag gaggccagcc cttatgcctc
     4201 gagctcattc tcaggatttg cgggatcggg tgatcgatgc ggtggtggag ggcggcgaga
     4261 gccgtcggtc ggcggcgcga cgttttgggg tgagcgaggc gtcggcggtg aagtggcttc
     4321 agcgagtgac ccggtttggc gaccggcgct gcggacgcat gggcggctat cggcgctcga
     4381 agctggagcc gcatcgcgag tggctgctgg cggcgatcgc ggccgagccg gacctgacgc
     4441 tggcggcgct gtgcgaaagg ctggcggcgg atcgggacct caagaccgac accggcacgc
     4501 tgtcgcgctt cttcgccagc gagggcatca gcttcaaaaa aaagcgtgct gcccagcgag
     4561 caggaccggc gggacgtggc gcggcgccgg gcgagctgga agcactacca ggaccggctt
     4621 gaccctcggc gcctcgtctt catcgacgag acctgggcca agaccaacat gctgcgcacc
     4681 cacggccgct gccggcgggg cgagcgcctg agggccaagg cgccgcacgg ccactggaag
     4741 acgatgacct tcatcgcggc gctccgccac gaccggatcg acgcgccgtg cgtcctcgac
     4801 ggaccgatca acggcatcag ctttaccgcc taggtcgagc agatgctgat cccgacgctc
     4861 aagcccggcg acatcgtcat cctcgacaat ctcggcagcc ataaaggcga agccgtgcgc
     4921 aaggccgtcc gagccgccgg cgccaggctc ctcttcctgc cgccctacag ccccgacctc
     4981 aatccaatcg agcaggtctt cgcgaagctc aagaccctgc tgaggaaagc cgccgagcga
     5041 accgtcgagg caacctggcg ccgcatcgga accctcctcg accgcttcac cccagaagaa
     5101 tgcgccaact acatcgctaa tgccggctat gcatcaatct aaatgcagaa tgctctagat
     5161 cagggccagc gccgaaagct tgagtgcgag ccgcggggga agga
//
LOCUS       DASWNU010000470         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_355651, whole genome shotgun sequence.
ACCESSION   DASWNU010000470 DASWNU010000000
VERSION     DASWNU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355651"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGB57_09215"
     CDS             complement(<1..552)
                     /locus_tag="VGB57_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0131572.1"
                     /translation="MKLLVPVKRVIDYNVKPRVKADGTGVDLANVKMSMNPFDEIAVE
                     EAIRLKEKGVATEIVAVSIGPAKAQETLRTALAMGADRAILVTTEDAVEPLAVAKILA
                     KIAAEEQPGMVILGKQAIDDDSNQTGQMLAALLGWAQGTFASKVEVVGERVSVTREVD
                     GGLETLSLKLPAIVTTDLRLNEPR"
     gene            complement(669..1868)
                     /gene="sucC"
                     /locus_tag="VGB57_09220"
     CDS             complement(669..1868)
                     /gene="sucC"
                     /locus_tag="VGB57_09220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY0131573.1"
                     /translation="MNVHEYQAKELLAKQGIPVPAGYAALSVEGAVEAAEKLPGPLWI
                     VKAQIHAGGRGKGKFRELGSEAKGGVRLARSIDEVRLNAAEMLGKTLVTIQTGAEGKQ
                     VNRLYVTDGVDIAREYYLALLVDRGSGRIAMVASTEGGMDIETVAHDTPEKITTVTID
                     PAQGFMPHHGRALAFALGLEGEQNKQGQKLARQLYDAFVALDCAMLEINPLVATEDGK
                     MLVLDAKMSFDSNAEFRHPEYEALRDESEEDPMEVEASKFDLAYIKLDGNIGCMVNGA
                     GLAMATMDIIKLNGAEPANFCDVGGGASKEKVTAAFKIILSDPAVKGILVNIFGGIMR
                     CDIIADGIIAAAKEVDLKVPLVVRLEGTNVEEGKRILADSGLAIVPANDLGDAARKIV
                     AAVKEAA"
     gene            1960..2367
                     /locus_tag="VGB57_09225"
     CDS             1960..2367
                     /locus_tag="VGB57_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131574.1"
                     /translation="MPTTIFDWIVRTALLAMAGLVTLSILGAIATMTNDPGTGFAGGD
                     RPLVEAGPAAEREAAQAPPQPDKDGNLAAAGPAGGEGGTIVAARPAPEDKVARWLEAI
                     AYALLAIAGIGALAVLMLWSLVRELRRIGRQAP"
     gene            complement(2469..>2860)
                     /locus_tag="VGB57_09230"
     CDS             complement(2469..>2860)
                     /locus_tag="VGB57_09230"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEY0131575.1"
                     /translation="ESSSRPIFLGGDLAAFWRIGGARVAKLGARGGFSHPFTGSALPD
                     ALRTSLALARQGDLSGMALHDLFEAEAAALWKRREFHRSVNRLLLDKGAPALGALYDL
                     DAALLERFFAEEVGMFDRRKILAAVGG"
BASE COUNT          424 a          968 c          959 g          509 t
ORIGIN      
        1 gcgcggctcg ttcagccgga ggtcggtggt gacgattgcc ggaagcttga ggctcagcgt
       61 ctcgaggccg ccgtcgacct cacgcgtcac cgagacacgc tcgcctacga cttcgacctt
      121 cgaggcgaag gtgccctgcg cccagccgag caaggcggcg agcatctggc cggtctggtt
      181 ggaatcgtcg tcgatcgcct gcttgccgag gatcaccatc ccgggctgct cctcggcggc
      241 gatcttcgcg aggatcttgg ccacggcgag cggctcgacc gcgtcttcgg tggtgacgag
      301 gatcgcccgg tcggcgccca tggcgagcgc ggtcctcaag gtctcctggg ccttggccgg
      361 cccgatcgag acggcgacga tctcggtcgc gacgcccttc tccttgagcc ggatcgcctc
      421 ctcgaccgcg atctcgtcga acgggttcat gctcatcttg acgttggcga ggtcgacgcc
      481 ggtcccgtcc gccttgaccc gcggcttgac gttatagtcg atcacccgtt tcacggggac
      541 gagaagtttc attccttcac ccttctcctt ttccgtcacc ccagcgaagg ctggggtccc
      601 ggggagtgaa gagcacggcg ctttcacccg ctggggcccc agccttcgct ggggcgacgt
      661 tccaaatcct aagccgcttc cttcaccgcc gcgacgatct tcctcgccgc gtcgccgagg
      721 tcgttggcgg ggacgatggc gaggccggag tcggcgagga tgcgcttgcc ctcctcgacg
      781 ttggtgccct cgagccgaac caccagcggc actttcagat ccacttcctt cgccgccgcg
      841 atgattccgt cggcgatgat gtcgcagcgc atgatcccgc cgaagatgtt gacgaggatg
      901 cccttcaccg ccggatcgga gaggatgatc ttgaacgccg ccgtcacctt ctccttggag
      961 gcgccgccgc cgacgtcgca gaaattggcg ggctcggcgc cgttcagctt gatgatgtcc
     1021 atcgtcgcca tggcgaggcc ggcgccgttc accatgcagc cgatatttcc gtccagcttg
     1081 atgtaggcga ggtcgaactt cgacgcctcg acctccatcg gatcctcctc gctctcgtcg
     1141 cgaagcgcct catattcggg atggcggaac tcggcgttgg agtcgaagct catcttggcg
     1201 tcgaggacga gcatcttgcc gtcctcggtc gcgaccagcg ggttgatctc gagcatcgcg
     1261 caatcgagcg cgacgaaggc gtcgtagagc tggcgggcga gcttctggcc ctgcttgttc
     1321 tgctctccct ccagcccgag cgcgaatgcg agggcacggc cgtggtgtgg catgaagccc
     1381 tgggcggggt cgatcgtgac ggtggtgatc ttctccggcg tgtcgtgcgc gaccgtctcg
     1441 atgtccattc cgccctcggt agaggcgacc atcgcgatcc gcccgctgcc gcggtcgacg
     1501 agcagggcga gataatattc cctggcgatg tcgaccccgt cggtgacgta gagccggttg
     1561 acctgcttgc cctcggcgcc ggtctggatg gtgacgaggg tcttgccgag catctccgcg
     1621 gcgttcaatc gcacctcgtc gatcgaccgg gcgagccgta cgccgccctt ggcttcggag
     1681 ccgagctccc tgaacttgcc cttgccgcga ccgccggcat ggatctgcgc cttgacgatc
     1741 cagagcggcc ctgggagctt ctccgccgcc tcgacggctc cttccaccga aagcgccgcg
     1801 tagccggcgg gaacgggaat gccctgcttg gcgagcaatt ccttggcctg atattcatgg
     1861 acgttcatgg ccgcgcccta aagcacaggg gcgaagcgat tccaagccgc gtgctcaggc
     1921 cttggaacat cgcccgcgtc agccgattgg aagcgcgtca tgccgaccac gatcttcgac
     1981 tggatagtgc gcaccgcgct gctggcgatg gcgggcctgg tcactctctc gatcctcggc
     2041 gccatcgcca ccatgaccaa cgatccgggg accggcttcg ccggcggcga ccggccgctt
     2101 gtcgaggcgg gcccggcggc ggagcgcgaa gcggcgcagg ctccgccgca accggacaaa
     2161 gacgggaatt tggctgccgc cgggccggcc ggaggcgagg gcggcacgat cgtcgcggct
     2221 cgccccgctc cggaggacaa ggtcgcgcgt tggctggagg cgattgccta cgcccttctg
     2281 gcgatcgccg ggatcggcgc gctcgcggtg ctgatgctgt ggagcctggt gcgagagctt
     2341 cggagaatag gtcgccaggc gccttgatgc atcacgtcgc cccggcgaag gctggggccc
     2401 ggggggtgaa gaactcggcg cttatcaccc gccgggatcc cagccttcgc tgggatgacg
     2461 ggtcccgctc agcctcccac ggccgccagt atcttgcgcc ggtcgaacat cccgacctcc
     2521 tcggcaaaga aacgttcgag cagggcggcg tcgaggtcgt agagggcgcc cagcgccggg
     2581 gcccccttgt cgagcaggag ccggttgacg cttcggtgga attcgcggcg cttccacagg
     2641 gccgcggctt ccgcttcgaa caggtcgtgg agcgccatcc cggaaaggtc gccctgccga
     2701 gccaaagcga ggctggtccg aagagcgtcg ggaagcgcgg agccggtgaa agggtgcgag
     2761 aagccccccc gcgctccgag cttcgccacc cgggctccgc cgatccgcca gaaggccgcc
     2821 aggtcccctc ccaggaagat cggccgcgag gacgattcgg
//
LOCUS       DASWNU010000471         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_227844, whole genome shotgun sequence.
ACCESSION   DASWNU010000471 DASWNU010000000
VERSION     DASWNU010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227844"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3844)
                     /locus_tag="VGB57_09235"
     CDS             complement(<1..>3844)
                     /locus_tag="VGB57_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131576.1"
                     /translation="KTGGEIFVSNPDPNFPAERQFNADSDVAVLASGTIVITWTKAVG
                     YGVDVYAQALTPAGVETGPEFRVHAHTDAYQQSPSITALASGGYVVIWEDGLEYEGGL
                     LGQAFDSSGAPAGNLFRVTTTVWGDQGYAAITALPGGGFVAVWDNYDTDYGTHSTRGQ
                     IFTGAGVKVGPEFRVSADSEWMQYPAAIDDLPGGGFVVTWYDRGVQYGDSSEGAIMAQ
                     VFDSAGAKVGNPFLVNSETSGNQNMPAVVALASGGFAVAWSSDSAGGYDVKAQIFMPL
                     TGAPTDLALSPGAVLEASISNYAVATFSHNGGVNSPMTYTLLADSTDGGFRIEGNRLV
                     VADNRRLDHETAPTASLTVRVTDVNGNSYQEALSVVIADSANEERYSAGQDFTADTST
                     TGGQGMAAIGALASGGYVMVWTDRGSDGSGGVVRAQMFGADGAKAGMEIGAPAAWDHG
                     GLATAGLASGGFVVVWKDFSPTGGDSSGSSIKGRRFDGSGAQAGGEFLVNTTTDYYQE
                     EPSVAALSSGGFVVTWTDNSPRATGAYDSDIRAQIYDSAGAKVGGEFVVNSTTFYEQT
                     DPVVASLPGGGFVIAWSDYPSHGVSAQRFDAAGNKVGGEILVGSSPWAGPNPAIAVLA
                     SGGFVISWYGDQLVAQIFDSTGAKSGAAFVVSEIGYGHSPTISALPSGGFVLAWQEGG
                     GAQADVRAQRFDSAGARIGGEFVVPSQSEGSRFGAVSTTLSSGSFVVGWTESGAVSAV
                     EGRVFSLIQPDNSGDDILTGDSNANLLDGGAGNDQIHGLAGNDDLRGGDGDDILDGGA
                     GNDVVSGGYGDDTAYVSGGTTSADTGTETVDLGFNLSGPGGGEEAGTDHLIVDYSNIT
                     EAVQFAHFGPSHGPAGASATIQIGGVERLSFSGVGRLTLTTGSGGDLVYAIGRDDIIS
                     TGAGNDIIHAFDSASGGRHRIDGGTGIDVAFRVNWSDLEVAVVLDLNDPQGVTIGSGA
                     GERYLRGIEAVVGFTSGSGDDVVVLDSAGALGNLVSTGLGSDTVVVFGGDTYVSTGQV
                     AVNLGAGPGTDELFADFSAATESVYFQSEYSGGTVSIGGVQRHGYSGAERLFLQTGSA
                     DDTVDGREGGTVAYGFSTGAGDDLLYGGELDDVLQGGIGNDRLEGGAGHDRLYGDGGN
                     DQLLGGAGNDWLSVEEAGTVTADGGGDSDTLTIVYVEATTAITMSAPAADPNGGLAGT
                     LGDGVGRSVTYGGIEQFVVLTGSGNDIVGGGAGDDYFNLGGGSDLLYYTGGTDYADGG
                     EGIDGIFGVYGTGGVGIVW"
BASE COUNT          592 a         1328 c         1294 g          630 t
ORIGIN      
        1 tccagacgat cccgacccct ccggtgccgt acaccccgaa aataccgtcg atcccctcgc
       61 cgccgtccgc ataatcggtg ccgccggtat aatataagag atcgctgccg ccgccgaggt
      121 tgaaataatc gtccccggcc ccgccgccga cgatgtcgtt gcccgacccg gtcaggacga
      181 cgaattgctc gatcccgcca taggtcacgc ttcggccgac gccgtcgccg agcgtgccgg
      241 caaggccgcc gttcgggtcg gcggcgggcg cgctcatcgt gatcgcggtc gtcgcctcaa
      301 catatacgat ggtcagagtg tcggaatcgc cgccgccgtc ggccgtgacg gttccggcct
      361 cctcgacgct cagccagtcg ttgccggcgc cgccgagaag ctggtcgttg ccgccgtcgc
      421 catagagccg gtcgtggccg gcgccgccct cgagcctgtc gttgccgatg ccgccctgaa
      481 ggacgtcgtc aagctctccg ccgtaaagca ggtcgtctcc cgcccctgtc gagaagccgt
      541 aggcgacagt gccgccctcg cgcccgtcga ccgtgtcgtc ggccgagccg gtctgaagga
      601 agaggcgctc ggcgcccgaa taaccgtgcc tctgcacgcc gccgatcgaa accgtgccgc
      661 ctgaatattc ggactggaag taaaccgatt cggtggccgc gctgaaatcg gcgaacaatt
      721 cgtcggtccc ggggcccgcg cccagattga ccgccacctg ccccgtcgaa acgtaggtgt
      781 cgccgccgaa gacgacgacc gtgtcgctgc cgagtccggt ggacaccaga ttgcccagcg
      841 ccccggccga atcgaggacg acgacgtcgt cgccgctgcc gctggtgaag ccgacgacgg
      901 cctcgattcc gcgcaggtaa cgctcgcccg cgcccgatcc tatcgtcacg ccctgcggat
      961 cgttgaggtc gagcacgacc gccacctcga ggtcgctcca gttcacccgg aacgccacgt
     1021 cgatcccggt cccgccgtcg atccggtgcc gcccgccgga ggcgctgtcg aaagcgtgga
     1081 tgatgtcgtt gccggccccg gtggagatga tgtcgtcgcg gccgatggcg tagacgaggt
     1141 cgccgcccga gccggtggtc agggtcagcc gcccgacccc gctgaagctc agccgttcga
     1201 ctccgccgat ctggatcgtg gcggaggcgc cggcgggacc gtggctcggg ccgaagtggg
     1261 cgaactggac ggcctcggtg atgttggaat agtcgacgat gagatggtcg gtgcccgcct
     1321 cctcgccgcc gccggggccg gacaggttga atccgaggtc gaccgtctcg gtcccggtgt
     1381 cggccgaggt ggtgccgccg ctgacatagg cggtgtcgtc gccatagccc ccgctcacca
     1441 cgtcgttgcc ggcaccgccg tcgagtatgt cgtcgccgtc gccgccgcga agatcgtcgt
     1501 tgccggcgag gccgtggatc tggtcgttgc cggcgccgcc gtcgagaagg ttggcgttgg
     1561 agtcgccggt caggatgtcg tcgccgctgt tgtcgggctg gatgagcgag aagacgcgcc
     1621 cttcgaccgc gctcacggcc ccggactcgg tccagccgac gacgaagctc ccggaggaga
     1681 gggtcgtcga aaccgctccg aaccggctgc cctcggactg cgatgggacg acgaactccc
     1741 cgccgatcct ggcgcccgcg gagtcgaagc gctgcgcgcg aacgtctgcc tgggctccgc
     1801 caccttcctg ccaggcgagg acgaacccgc ccgacggcag ggcgctgatc gtgggtgagt
     1861 ggccgtaccc gatctcgctc acgacgaacg ccgcacccga tttcgcaccg gtggaatcga
     1921 agatttgggc cacgagctgg tcgccgtacc aactgatcac gaacccgccc gaggcgagca
     1981 ccgctatcgc cgggttcggg ccggcccaag gcgatgatcc caccaggatc tcgccgccga
     2041 ccttgttgcc ggcggcatcg aaccgctggg cggagacgcc gtgactggga taatcggacc
     2101 atgcgatgac gaacccgccg cccggcaggg aagcgacgac cggatcggtc tgttcgtaga
     2161 aggtggtgct gttgaccacg aattccccgc cgaccttcgc acccgccgag tcgtagatct
     2221 gcgcccggat atcgctgtcg taagcgccgg tggcgcgagg gctgttgtcg gtccaggtga
     2281 cgacgaagcc gccggaggaa agcgccgcca ccgacggctc ctcctggtag tagtcagtgg
     2341 tggtgttgac caggaactct ccgcccgcct gcgcccccga gccgtcgaac ctccgcccct
     2401 tgatgctcga accgctggag tcgccgccgg tcgggctgaa atccttccag acgacgacga
     2461 agcctcccga tgcgagtccg gccgttgcga gacctccatg gtcccaggcc gcgggggcgc
     2521 cgatctccat acccgccttg gcgccgtcgg cgccgaacat ctgggccctg acgaccccgc
     2581 cgctgccgtc gctgccccga tccgtccaga ccatgacata accgcccgag gcgagcgctc
     2641 cgatcgccgc catgccttgg ccgccggtcg tggaggtatc ggcggtgaag tcctgccccg
     2701 cgctgtaccg ctcttcgttt gccgagtcgg cgatgacgac gctgagcgcc tcctggtagc
     2761 tgttgccgtt cacgtccgtg accctcacgg tcaggctggc ggtgggcgcc gtctcgtgat
     2821 cgagccggcg gttgtcggcg accacgagcc tgttgccttc gatccggaag ccgccatcgg
     2881 tcgaatcggc gaggagcgtg taggtcatgg ggctgttgac gccaccattg tgcgagaagg
     2941 tcgccaccgc ataattggag atgctggctt cgagcacggc gcccggcgac aaggcgaggt
     3001 cggtgggcgc gccggtcagg ggcatgaata tctgcgcctt gacgtcatag cctccggcag
     3061 agtcgctcga ccaggcgacc gcgaagccgc ccgaggcgag ggcgacgacg gccggcatgt
     3121 tctggtttcc gcttgtctcg ctgttgacga ggaatgggtt gcccaccttc gctccggcgg
     3181 agtcgaagac ctgcgccatg atcgcgccct cggatgagtc gccgtattgg acgccgcggt
     3241 cgtaccaggt gacgacgaag ccgccgcccg gaaggtcgtc gatcgcggcc ggatactgca
     3301 tccactcgct gtccgcgctg actcggaatt ccggccccac cttcacgccc gcaccggtga
     3361 agatctggcc ccgggtggag tgagtcccgt aatcggtgtc gtaattgtcc cagacggcga
     3421 cgaagccgcc gcccggcaaa gccgtgatgg cggcgtaccc ctggtcgccc cagacggtgg
     3481 tggtcacccg gaacaggttg cccgccggcg ctccgctgga atcgaacgcc tgcccgagga
     3541 gtccgccctc atattcgaga ccgtcttccc agatgacgac atagccgccg gaagcgaggg
     3601 cggtgatgga gggtgactgc tgataggcgt cggtgtgcgc atggacccgg aactcgggtc
     3661 cggtttcgac gcctgcggga gtcagggcct gggcgtagac gtcgaccccg tagccgaccg
     3721 ccttggtcca ggtgatcacg atcgtgccgg aggcgagcac ggcgacgtcg ctgtcggcgt
     3781 tgaactgccg ctcggcgggg aaattcgggt ccgggttgga aacgaagatc tccccgcccg
     3841 tctt
//
LOCUS       DASWNU010000472         5963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_122946, whole genome shotgun sequence.
ACCESSION   DASWNU010000472 DASWNU010000000
VERSION     DASWNU010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122946"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2168)
                     /locus_tag="VGB57_09240"
     CDS             complement(<1..2168)
                     /locus_tag="VGB57_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0131577.1"
                     /translation="MLKKTSAPDLDALRLLEERLRWLSAWTIHNANHLRTSPDGLKVG
                     GHQASCASMTAIMAALYFHALGPNDKVAVKPHAGPVLHAVHYLLGSQSREQLENFRGF
                     GGMQSYPSRTKDRIPVDFSTGSVGLGVAVTAFASLVQDYLVAHGDLAPADTGRMVALM
                     GDAELDEGNIYECLIEAYKHDIRNCWWIVDYNRQSLDATTAERMFRRFDEIFASCGWR
                     VVTLKYGKALEAAFAEPGGEALRNWLEECGNADYAALTYLGGAAWRERLLKDSPETKP
                     LLDARDDEALAALMTNLAGHDMASLVEAFDSARDDVPTLFIAYTIKGFGLPFAGHKDN
                     HAGLMNPGQVAALRSAMGIAEGEEWEPWAGLGGNAAEGVRALVEDSRIARAKRERPWH
                     QVEVPAIPAPAGEEQSTQAAFGRILLDLSRAGGGLADRIVTTSPDVTVSTNLGAWVNQ
                     RGLFRRQDLADVFAAAKIPSAQKWGATDAGQHIELGIAENNLFLMLAALGLAGDLFGT
                     RLFPIGTVYDPFIARGLDALNYACYQDARFLLVATPSGVTLGPEGGAHQSINPPLIAL
                     GQPGLRHYEPAYVDELALLMEEGFRLMQAPDGDSLYLRLTTRSIPQIERADDSWKDGA
                     LAGGYWLREPGPGAEAALVYSGAIAPEALAAWEALKYDVPGLGLLAITSPDLLHRGWS
                     ERRSARWGGRGAQPSHVEKLLAPLAPGAGLVTLLDGAPAAL"
     gene            complement(2224..2715)
                     /locus_tag="VGB57_09245"
     CDS             complement(2224..2715)
                     /locus_tag="VGB57_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131578.1"
                     /translation="MPSPPVIAFTPVPLRPRADGWSPDLQLRFIVALSRGLAPGEAAR
                     SVGKNRQNAYALRKRPGAESFAAAWDSVVAYVQHVRAQGRLPRPAPVASSPAQPSFRR
                     PRGAEAEAIAERGAEAMKRAPTEVAARRALDDMLDALYGPKSDNSDNDGSGKKPPMGS
                     RNL"
     gene            complement(2719..2991)
                     /locus_tag="VGB57_09250"
     CDS             complement(2719..2991)
                     /locus_tag="VGB57_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131579.1"
                     /translation="MSRFPFLHFIPVPVRPRRDGWTPVLQIRFVIGIARGLSPARAAA
                     RIGKTRQSALNLRKHPEGRSFAAAWESARRFADEARAGKDAPPGPV"
     gene            complement(3166..4788)
                     /locus_tag="VGB57_09255"
     CDS             complement(3166..4788)
                     /locus_tag="VGB57_09255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY0131580.1"
                     /translation="MTLDLDRRLLLKAGTLGLGALAAPGAAQIASRVGFTHGVASGEP
                     RQRSVMLWSRHVPIRGDSSRLGWEVTEPGKRRVVAGGPVLATAGSDWCVKVVAEGLEP
                     GRWYDYRFLNLDGAAAATGRTRTLPEGRTPRFTLGIFSCANLAFGHFNAYAHAAERRD
                     LDLLVHLGDYYYEYHSGKYPSAREAVAGRLLDPVHEAVALADYRLRLAAYRSDPDLRR
                     LHASAPMVMMWDDHETANDSWSGGAENHDPAKEGPWSARKAAARRAYREWLPVSDNDW
                     ESYEIGDLATLFRPETRLTARSRPLQFGEALRRGEDLKASLARFRDGPWRDPERTMLG
                     GEQESWLAGALKRSTGQGTRWQLLAQQTIMGSFALPLEARTWLRSDSPEEVKSVTAIG
                     AAASEVGLPLNLDAWDGYPAARDRLLRSALDADSNLVVLSGDSHNGWAFDLDLAGTPA
                     GAEFAGMSVTSPGLEAYTPGVAPAEVARALRARNPALKWAELQRRGYMTLTLTRERAT
                     GEWLSLDTVRTRSTKLAARHSMSVARGANRFA"
     gene            complement(4952..>5963)
                     /locus_tag="VGB57_09260"
     CDS             complement(4952..>5963)
                     /locus_tag="VGB57_09260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0131581.1"
                     /translation="RRQGERREAGAPASPGEAKGFNPQPDPPKNKGPVSAGDLVGLNP
                     QPEPPGLERLKILASPAVRARARDLGIDLAQVKTTADRIRHSDLDAWLLYNGGGVSRS
                     AGQRRQDETVKVVGLRRKIAENMQEAKRRIPHFTLVEEYDVTALEQTRSMMNSDRGGN
                     PKLTLLPFLITAMARTLGDYPQINTRYDDDENLLHRSGAVHMGMATQTPNGLMVPVIR
                     GADRLSIWDIAREILRLSEAARTGKASRDELSGSTITISSLGPMGGIASTPVINRPEV
                     AILAVNKVEEKPVVIEGELEIRKRMNLSLSCDHRVVDGWDAANFMQDLKKLVENPLKL
                     LS"
BASE COUNT          880 a         1897 c         2245 g          941 t
ORIGIN      
        1 agagcggccg gcgcgccatc gagcagggtg accagcccgg ccccgggcgc aagcggcgcg
       61 aggagcttct cgacgtgaga cggctgagcc cccctgccgc cccagcgcgc gctccgccgc
      121 tcgctccagc cgcggtggag aagatcgggc gaggtgattg cgaggaggcc gaggcccggc
      181 acgtcgtact tcaacgcttc ccatgcggcg agcgcttccg gggcgatcgc gcccgaatag
      241 accagagcgg cttcggcgcc ggggccgggc tcgcgcagcc agtagccgcc ggcgagcgcg
      301 ccgtctttcc agctgtcgtc cgcgcgctcg atctggggga tgctgcgggt ggtgaggcgc
      361 aggtagaggc tgtccccgtc gggcgcctgc atcaggcgga agccttcctc catcagcaac
      421 gcaagttcgt cgacataggc cggttcgtag tggcgaaggc cgggctggcc gagggcgatc
      481 aagggcgggt tgatcgactg gtgggcgccg ccctcgggcc cgagggtgac acccgacgga
      541 gtcgcgacga gcaggaagcg ggcgtcctgg tagcaggcgt aattgagggc atcgaggccg
      601 cgggcgatga acgggtcgta gacggtgccg atcgggaaca gccgggttcc gaacaggtcg
      661 ccggcaagac ccagggcagc gagcatcagg aacagattgt tctcggcgat gccgagctcg
      721 atatgctggc cggcgtccgt ggcgccccat ttctgggccg aggggatctt ggcggcggcg
      781 aagacgtcgg ccaggtcctg gcggcggaac aggccgcgct ggttcaccca ggcgccgaga
      841 ttggtcgaga cggtgacgtc gggcgaggtg gtgacgatcc ggtcggcgag cccgccgccg
      901 gcgcggctga ggtcgagcag gatccggccg aacgccgcct gggtcgactg ctcctcgccg
      961 gccggcgcgg gaatggccgg gacctcgacc tggtgccagg gccgctcgcg cttcgccctc
     1021 gcgatccggc tgtcctcgac cagggcccgc acgccttccg ccgcatttcc gccgagcccg
     1081 gcccagggct cccattcctc cccttccgcg atccccatcg ccgagcgcag ggcggcgacc
     1141 tggccggggt tcatcaggcc ggcatgattg tccttgtggc cggcgaaggg caggccgaag
     1201 cccttgatcg tgtaggcgat gaagagggtc ggcacgtcgt cccgcgcgga gtcgaacgcc
     1261 tcgacgaggc tcgccatgtc gtggccggcg aggttggtca tcagggcggc gagcgcctcg
     1321 tcgtcgcggg cgtcgaggag cggcttcgtc tcgggcgagt ccttcagcaa tcgctcccgc
     1381 caggcggcgc cgccgagata ggtgagcgcg gcgtaatcgg cattgccgca ctcttcgagc
     1441 cagttcctga gcgcttcgcc gcccggctcg gcgaacgccg cctccagcgc cttgccgtat
     1501 ttcagcgtga ccacccgcca gccgcaactc gcgaagatct cgtcgaagcg gcggaacatc
     1561 cgctcggcgg tggtcgcatc cagggactgg cggttgtagt cgacgatcca ccagcaattg
     1621 cggatgtcgt gcttgtaggc ctcgatcagg cattcgtaga tattgccctc gtcgagctcg
     1681 gcatcgccca tcagagcgac catccgcccg gtgtcggccg gcgccagatc gccatgggcg
     1741 acgagatagt cctggacgag gctggcgaag gcggtgaccg ccaccccgag gccgaccgag
     1801 cctgtcgaga aatcgaccgg gatccggtcc ttggtccggc tcggatagct ctgcattccg
     1861 ccgaagcctc ggaaattctc gagctgctcg cggctctggc tgccgagcag ataatggacg
     1921 gcgtggagca ccggcccggc gtggggcttg accgcgacct tgtcgttggg gccgagcgcg
     1981 tgaaaataga gcgccgccat gatcgccgtc atcgaggcgc agctcgcctg gtggccgccg
     2041 accttgaggc cgtcggggct cgtccgaagg tggttggcgt tgtggatcgt ccaggcggaa
     2101 agccagcgca gccgctcttc gagcaggcgg agggcgtcga gatcgggggc ggaggtcttc
     2161 ttcagcatgg cgggcgcctt agcgggcgag gcggtgaaaa gcgacaagag cgacacttgg
     2221 ggttcataag tttcggcttc ccataggagg cttttttccg ctgccgtcat tgtcgctgtt
     2281 gtcgctcttg gggccgtaca aggcgtcgag catgtcatcg agggcgcgtc gggcagcgac
     2341 ctccgtgggc gcccgcttca tcgcctccgc gccgcgttcg gcaatggctt cggcttcagc
     2401 ccctcgcggc cgcctgaagc tcggctgcgc aggcgaagag gccaccgggg cgggccgcgg
     2461 cagccgaccc tgcgcccgaa cgtgctgcac gtatgccacg acggaatccc aggcggcggc
     2521 gaagctctcc gcgcccggcc gcttcctcaa ggcgtaggcg ttctgccggt ttttccccac
     2581 cgaccgcgcc gcctcccccg gagcgaggcc acgcgacaga gcgacgatga agcgaagctg
     2641 caggtcgggc gaccagccgt cggcgcgcgg gcggagcggc actggggtga aggcgatgac
     2701 ggggggcgag ggcatcgctc aaacgggccc cggaggagcg tccttaccgg cgcgagcctc
     2761 atccgcgaac cgacgcgcgg attcccacgc ggccgcgaaa ctccttccct ccgggtgctt
     2821 gcgcaggttg agggccgatt gcctggtctt gccgatgcgg gcggcggccc tggccgggct
     2881 caggccccgg gcgatgccga tgacgaagcg gatctggagc accggcgtcc agccgtcgcg
     2941 gcgggggcgg accgggacgg ggatgaagtg gaggaagggg aagcgggaca tgatcggagg
     3001 gtagcatagt cagaacaaaa agagaacagt tcacttgaat cctccccgcg acttcgcggg
     3061 gagggggacc gcgcgaagcg cggtggaggg gttctttcgg tctctggaag acccctccac
     3121 catgcttcgc atggtccccc tcccccggaa atccggggga ggaatttatg cgaagcggtt
     3181 ggcgcctctg gcgacgctca tcgaatggcg tgccgccaat ttcgtggagc gggtccggac
     3241 cgtgtcgagc gagagccatt cgccggtggc gcgctcgcgg gtgagggtga gggtcatgta
     3301 gccgcggcgc tggagctcgg cccatttgag agccgggttg cgggccctga gggcgcgcgc
     3361 cacctcggcg ggggcgactc cgggcgtata ggcttcgagg ccgggcgaag tgacgctcat
     3421 tccggcgaat tcggcgccgg cgggggtgcc cgcgaggtcg aggtcgaagg cccagccatt
     3481 gtggctgtcg ccggagagga cgacgaggtt cgaatcggcg tcgagggcgg agcggagcag
     3541 gcggtcgcgg gcggcgggat agccgtccca ggcgtcgagg ttgagcggca ggccgacttc
     3601 ggacgcggcg gcgcctatcg cggtcacgct cttcacctcc tcgggcgagt cggagcggag
     3661 ccaggtccgg gcctcgagcg gcaaagcgaa gcttcccatg atcgtctgct gggcgaggag
     3721 ctgccagcgg gtcccctgcc cggtcgagcg cttcagggcg ccggccagcc aggattcctg
     3781 ctcgccgccc agcatcgtcc gctccggatc gcgccagggg ccgtcgcgga agcgcgccag
     3841 cgaagccttg aggtcctcgc cgcgccgaag cgcctccccg aactggagcg ggcggctgcg
     3901 cgccgtcagc cgggtctccg gcctgaacag ggtggcgaga tcgccgatct cgtagctttc
     3961 ccaatcattg tcggagaccg gcagccattc gcgataggcg cgccgggcgg cggccttgcg
     4021 cgcgctccac ggaccctcct tcgcggggtc gtgattctcg gcgccgcccg accaggagtc
     4081 gttggcggtc tcgtgatcgt cccacatcat aaccatcggc gcggaggcgt ggaggcgccg
     4141 gagatcggga tcggagcggt aggcggcgag gcgaagacga tagtcggcga gcgcgaccgc
     4201 ctcgtgcacg ggatccagaa gtcggccggc cacggcctcc cgggcggacg ggtatttgcc
     4261 gctgtgatat tcgtaataat agtcgccgag atggacgagc agatcgaggt cgcggcgttc
     4321 ggcggcgtgg gcgtaggcgt tgaaatggcc gaaggcgagg ttggcgcagg agaagattcc
     4381 gagggtgaaa cggggggtgc ggccctcggg aagggtgcgg gttcggccgg tggcggcggc
     4441 ggcgccgtcg agattgagga aacggtaatc gtaccagcgg cccggctcca gcccctccgc
     4501 gaccaccttg acgcaccagt cgctccccgc cgtggccagg actgggcctc cggcgacgac
     4561 tcggcgtttg ccgggctcgg tgacctccca gccgagccgc gacgaatcgc cgcgtatcgg
     4621 gacgtgacgg ctccacagca tcaccgagcg ctgccgcggc tcgccgctcg cgaccccgtg
     4681 ggtgaagccc acccgggagg cgatctgcgc agcgccgggt gcggcgagcg cgccaaggcc
     4741 gagggtgccg gccttgagca ggaggcggcg gtcgaggtcg agggtcattc gggagttgtg
     4801 acgggggaat ggggggattg gaaggtggaa ttccctctca cgtcattccg gcgaaggccg
     4861 gaatccatga acgcggccga acgaatcctc gcccctccgt gttcctggat cccggatcga
     4921 gtccgggatg acgggtagcg cagaccgccg atcagctgag taatttcagc gggttttcca
     4981 ccagcttctt caggtcctgc atgaaattgg ccgcgtccca gccgtcgacc acgcggtggt
     5041 cgcaggagag ggacaggttc atgcgcttcc tgatctcgag ctcgccctcg atcacgaccg
     5101 gcttctcctc gaccttgttg acggcgagga tcgcgacttc cgggcggttg atgaccgggg
     5161 tcgaggcgat tccacccatc ggcccgaggc tggagatggt gatggtcgag ccggacagct
     5221 cgtcgcggct cgccttgccg gtgcgggcgg cttcggagag gcggaggatc tcgcgggcga
     5281 tgtcccagat ggaaagccgg tcggcgcccc ggatgacggg gaccatgagg ccgttcgggg
     5341 tctgggtcgc catgcccatg tggacggcgc cggagcggtg gagcaggttc tcgtcgtcgt
     5401 cgtagcgggt gttgatctgc ggatagtcgc cgagggtcct ggccatggcg gtgatcagga
     5461 agggaagcag ggtgagcttc gggttgccgc cgcggtcgga gttcatcatc gagcgggtct
     5521 gctcgagcgc ggtgacgtca tattcctcga ccagggtgaa atgcgggatc cggcgcttgg
     5581 cctcctgcat attctccgcg atcttgcgtc tgaggccgac gaccttgacg gtctcgtcct
     5641 ggcggcgctg gccggcggag cgcgagacgc cgccgccatt gtagagcagc caggcgtcga
     5701 ggtccgagtg acggatgcga tcggcggtcg tcttcacctg ggcgagatcg atgccgaggt
     5761 cgcgggctcg ggctcggacg gccggggagg cgaggatctt cagcctctcc agccccggcg
     5821 gctcgggctg ggggttgagg ccgaccaggt cgcccgcgga aaccgggccc ttgttcttgg
     5881 gcggatcggg ttgcgggttg aagcccttgg cttcgccggg gctggcgggc gcgccggctt
     5941 ccctccgctc cccctgccgg cgg
//
LOCUS       DASWNU010000473         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_200874, whole genome shotgun sequence.
ACCESSION   DASWNU010000473 DASWNU010000000
VERSION     DASWNU010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200874"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="VGB57_09265"
     CDS             complement(<1..1348)
                     /locus_tag="VGB57_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0131582.1"
                     /translation="MLGDLPDLIARRAELTPDSVALEELATGRRLTYAALDERAARAA
                     ALLEARGVGDGDRVAILCRNRLAFFEALFACARLGAILVPLNWRMPPAELDPLLAHCG
                     PRLLLHGGEDSAAVRRLAAPPPAIDLDGDYEALVEASAPSARRGRWPADAIWYLLYTS
                     GTTGRPKAVIYSYKMALANLVNIGTAIGLGASDTTLSFLPNFHTAGINLHALPTLLQG
                     GRVLLLPGFDADSVVELLEARRVDTFFAVPAVYQALVDHPRFAAAPLGHVRHWGCGGA
                     PLPDVLARRCRDLGLRVCNGMGMTETGPTAFLAAPADAWDRIGSVGKPQLLVSVRIVD
                     EDGRDVADDEVGDLLFGGPAVTPGYWNDPEATRSAFTADGWLKSGDLARRDPDGFYWV
                     AGRRKEMFISGGENVYPAEVENVLAGHPAVTEAAVVAAPHSLWGEIGCAFLRLAEGR"
     gene            complement(1348..1971)
                     /locus_tag="VGB57_09270"
     CDS             complement(1348..1971)
                     /locus_tag="VGB57_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0131583.1"
                     /translation="MATIAANGDKEPRTERGRKTLRRLIEAAAAEFGERGFHEAAITG
                     ITRRAGVALGTFYTYFQSKEEVFRALVLDMSRATRAHVAEAVKGAPDRLAAERLGLEA
                     FIAFTRRHPELYRIIEEAQFVAHDVYREHYLSFADAYRRNLAGARERGEIADGGHGAR
                     EDEARGWALIGMSVFLGMRYGLWDEDLSPADVADIAIELVSEGLRKR"
     gene            complement(2038..2517)
                     /locus_tag="VGB57_09275"
     CDS             complement(2038..2517)
                     /locus_tag="VGB57_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY0131584.1"
                     /translation="MDQTIPVSTIAHLIQLAIAPVFLLAGIAGILNVLAQRLARVVDR
                     GRSLERDFASFDEATRERASEELLIIIRRMRIVNLAITACTASALFVCLVVAILFVAD
                     LAQIAFARPIAVLFIIAMGLLIVGLTFFLAEIRMAARFVSIRHDLLPLPGRERPTGE"
     gene            complement(2624..>3035)
                     /locus_tag="VGB57_09280"
     CDS             complement(2624..>3035)
                     /locus_tag="VGB57_09280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEY0131585.1"
                     /translation="RYSLLVERFASVGEQALVRPPFYCDHGYNISLGSGAFLNYNCII
                     LDVEPIVIGEGTAIGPAVQILASDHPRDPRLRRQKVRFGRPVTIGANVWIGGGAIILP
                     GVTIGDDAIVGAGSIVTRDVAAGVTVVGSPAREK"
BASE COUNT          509 a         1027 c         1064 g          435 t
ORIGIN      
        1 gccgcccctc cgcgagcctc aggaaggcgc atccgatttc cccccagagc gaatgcggcg
       61 cggcgacgac cgccgcctcc gttaccgccg gatggccggc gagcacattc tcgacctcgg
      121 ccggatagac gttctccccg cccgagatga acatctcctt ccggcgcccg gcgacccagt
      181 agaagccgtc cggatcgcgg cgggcgagat cgcccgattt caaccagccg tcggcggtga
      241 aggccgagcg ggtcgcctcc gggtcgttcc aatagcccgg agtcaccgcc ggcccgccga
      301 acagcagatc gccgacctcg tcgtccgcaa cgtcgcggcc gtcctcgtcg acgatccgga
      361 ccgagaccag gagctggggc ttgccgacgg agccgatccg gtcccatgcg tccgccggcg
      421 cggcgaggaa tgcggtcggg ccggtctcgg tcatccccat gccgttgcag accctgaggc
      481 cgaggtcgcg gcagcggcgg gcgagcacgt cgggcagcgg cgctccgccg cagccccagt
      541 gacggacgtg gccgaggggg gccgcggcga agcggggatg gtcgaccagc gcctggtaga
      601 cggcaggcac ggcgaagaag gtgtcgaccc tccgcgcctc gagaagctcc accaccgaat
      661 cggcgtcgaa gcccggcagg agcaggaccc ggccgccctg gagcagggtc ggcaaagcgt
      721 ggaggttgat cccggcggtg tggaagttgg gaaggaagct cagcgtggtg tccgacgctc
      781 cgaggccgat ggcggtgccg atgttgacca ggttcgcgag cgccatctta tagctgtaga
      841 tcacggcctt gggccgtccc gtcgtccccg acgtatagag cagataccag attgcgtcgg
      901 ccggccagcg gccgcgccgg gcgctcggcg ccgaggcttc gaccagcgcc tcatagtcgc
      961 cgtccagatc gatcgccgga ggcggcgcgg caagacggcg caccgccgcc gagtcctcgc
     1021 cgccgtgaag gagcaaccgg gggccgcaat gggcgagcaa agggtcgagc tcggccgggg
     1081 gcatccgcca gttgagcgga accaggatcg cgcccagccg ggcgcaggcg aacaaggcct
     1141 cgaagaaggc gaggcggtta cggcagagga tggcgacccg gtcgccgtcg cccactccgc
     1201 gagcctcgag caaggcggcg gcgcgggcgg cgcgctcgtc gagcgccgca taagtgaggc
     1261 ggcggccggt cgccagctcc tcgagggcca ccgaatccgg agtgagctcg gcgcggcggg
     1321 cgatcagatc gggcaggtcg ccgagcatca ccgcttcctc agcccctccg agaccagctc
     1381 gatggcgatg tcggcgacat cggcggggct cagatcctcg tcccacaggc cgtagcgcat
     1441 gccgaggaac acgctcatcc cgatgagggc ccagccgcgg gcctcgtcct cgcgcgcgcc
     1501 atgcccgccg tcggcgatct cgccgcgctc ccgggcgccg gccaaattgc gccgataggc
     1561 gtccgcgaag ctcagataat gctcgcgata gacgtcatgg gcgacgaact gcgcctcctc
     1621 gatgatccgg tacagctcgg gatgccttcg ggtgaaggcg atgaacgcct cgagcccgag
     1681 ccgttccgcc gcgagccgat ccggggcgcc cttgaccgcc tcggcgacat gggcgcgggt
     1741 cgcccggctc atgtcgagga cgagggcccg gaagacctcc tccttgctct ggaaataggt
     1801 gtagaaggta ccgagcgcga ctccggcgcg gcgggtgatg ccggtgatcg ccgcttcgtg
     1861 gaagccgcgc tcgccgaact cggccgcggc ggcctcgatc aggcgccgaa gcgtcttgcg
     1921 cccgcgctcc gtgcgcggct ccttgtcgcc attggcggca atcgttgcca tagcctctcc
     1981 ccggcccgcg aagttgaacc tgatttcacc tttgcgcaag ccggccgctc atccggccta
     2041 ttcccctgtc gggcgctccc gccccggcag cggcagcagg tcgtggcgga tgctgacgaa
     2101 gcgggcggcc atgcggatct cggcgaggaa gaaggtgagg ccgacgatca gcagtcccat
     2161 ggcgatgatg aaaagcacgg cgatcggccg cgcgaaggcg atctgcgcca ggtcggcgac
     2221 gaacaggatc gcgaccacga ggcagacgaa cagggcgctc gcggtgcagg cggtgatggc
     2281 gaggttcacg atccgcatcc gccggatgat gatgagcagc tcctcgctgg cgcgctcccg
     2341 ggtcgcttcg tcgaacgagg cgaagtcgcg ctcgaggctt cggcctcggt cgaccacccg
     2401 ggcaagacgc tgggcgagca cgttcaatat gccggcgatc ccggcgagca ggaagacggg
     2461 cgcgatcgcc agctggatga ggtgggcgat ggtcgagacg gggatggtct gatccatgtc
     2521 cgggccacac gctcccagcc cgcgagtgtc aaggctccgc gtagcggatc agctccgacc
     2581 gcgggctcgc gctgaatgtc tgcttccggc ccaaagcgga ccttcacttt tcccgcgcgg
     2641 ggcttccgac gacggttacg ccggcggcga catctcgagt gacaatgctc ccggcgccca
     2701 cgattgcatc gtctccgatc gtgacgcctg gcaggatgat cgcaccgcca cctatccaga
     2761 cattcgcgcc gatcgtcacg ggtcgaccga agcgcacttt ctgtcgtcgc aaacgggggt
     2821 cccgcgggtg atcggaggcg aggatctgaa cagccggacc gatggctgtc ccttcgccga
     2881 tgactatcgg ctcaacgtca aggatgatgc aattatagtt caggaaggct ccggacccca
     2941 gcgagatgtt gtatccatga tcgcaataga acggtgggcg tacaagggct tgctcgccga
     3001 ccgaagcgaa gcgctcgact agcaacgagt atctt
//
LOCUS       DASWNU010000474         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_252234, whole genome shotgun sequence.
ACCESSION   DASWNU010000474 DASWNU010000000
VERSION     DASWNU010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252234"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..793)
                     /locus_tag="VGB57_09285"
     CDS             complement(185..793)
                     /locus_tag="VGB57_09285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY0131586.1"
                     /translation="MCNLYSNKVAPDAIARTFEQLGMVLSFPEGLPNLQPREEIRITD
                     TAPIVRRRFAGAGEGGAELVQRRWSWAGPGGKPVYNFRSDGREFGNSKAGGRCLIVTD
                     GFYEFTTPKAPRADKRKDRWLFTMPGRDWFCIAGLWRTVGSGEAFTMLTTEPGPDVAP
                     YHHRQVAVLAHESWSAWLDGSGSALELLGPCPAGMLEVARVG"
     gene            880..955
                     /locus_tag="VGB57_09290"
     tRNA            880..955
                     /locus_tag="VGB57_09290"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:914..916,aa:Glu,seq:ctc)
     gene            1137..1541
                     /locus_tag="VGB57_09295"
     CDS             1137..1541
                     /locus_tag="VGB57_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131587.1"
                     /translation="MTDRALGTKHLEMLQAAISRLAGNAFLIKGWSIALATAVAGFAV
                     KDGASRVALVGIVPIALFWCLDSYYLSLERAFRALFDSTRAQVLADLPPTLDMTPSLA
                     PLAVVANAFRPAVILVHLPAQILLLAAAFAGS"
     gene            complement(1564..2169)
                     /locus_tag="VGB57_09300"
     CDS             complement(1564..2169)
                     /locus_tag="VGB57_09300"
                     /inference="COORDINATES: protein motif:HMM:NF036730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131588.1"
                     /translation="MKAIFLSASVPDPRRDPIYYETADLVAIRDCVRALATVVLPHAA
                     LFWGGHPAITPLIRVVAENAGIKAPDRIRLFQSAWFSDVLPADNAEFERYELTPRQAT
                     RDSSLHTMRQRMISAAAFDAGIFIGGMEGVEQEFAMFVKDHGKARLLPIASTGGAARL
                     IYEKEKRRLDLPRALEEDYAYPSLFRKLLDLPFEPSGGPSR"
     gene            complement(2166..3326)
                     /locus_tag="VGB57_09305"
     CDS             complement(2166..3326)
                     /locus_tag="VGB57_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY0131589.1"
                     /translation="MAEDALYQIVLLGPGCARHKDVFIAELTERLAEIGGDLEGALRV
                     LGASEIPALRKTAPSVAVYFGGGGAPTDEAAVGELIGMAVPILPVVDAVEDYAAKVPP
                     ALHPINGMAVAWDDPDFAAAINIALENLSLLRRRRRLFLSYFRKDSSVAAHQLRVAFD
                     DRGFDTFLDTSSVPKGDDFQSVLWHRLLDSDVMIVLDTADFLTSRWTRQELAQASAMS
                     VGMLRVVWPGVERVREAELAAHLYLDPADFLGDRLTDAAAERIIQATEVLRARCIAAR
                     HTNLVVEFCEEAERIGVHTAVQPERFILAQRGGGQRVAAIPAVGVPDAQLYHDAAGRF
                     PVAGERADAAVLIYDHRGMLPAWIQFLDWLDDHLPVKGLRVTETAAKLGGLL"
     gene            3654..4319
                     /locus_tag="VGB57_09310"
     CDS             3654..4319
                     /locus_tag="VGB57_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131590.1"
                     /translation="MLIALNSPSAPGTGPVLASVHLALDAVTPATVTVSYETLPGNQP
                     KLYGNFLAIWQSTVIPWSAPPLKRQALAPGGQSGATTLSGLGIQRKPYIVGYGVGAEI
                     TSIAASLALHPDREAVVLATTIEVPAITPDSLVIHYRTLPGYRPLTAGNWIGLWQGQA
                     SPFYSGDPIARAVPDHDFSEGYIAMDGLVLTFGTTYTLVYFAGAGKTEAAAILTFTTA
                     SRS"
BASE COUNT          774 a         1465 c         1419 g          729 t
ORIGIN      
        1 gcggtttcac aatgtcaaag agccgcgacc cggatctccg agtcgctgac cagtatggta
       61 ggaaaagtcc tacaagtcaa gaacaatatg agaacatccc gtcatcccgg cgaaagccgg
      121 gaccacgggg agacccgccg agccttctcg tgccgaggtc ccggctttcg ccgggatgac
      181 ggaattatcc gaccctcgcc acctccagca tccccgccgg gcacggcccg agcagctcga
      241 gcgccgagcc gctgccgtcg agccacgcgc tccagctctc atgggcgagc accgcgacct
      301 ggcggtggtg gtagggcgcg acgtccggcc ccggctcggt ggtcagcatg gtgaaggctt
      361 ccccggaacc gacggtccgc cacaggccgg cgatgcagaa ccagtcccgg cccggcatgg
      421 tgaacagcca acggtccttg cgcttgtcgg cgcgcggcgc cttcggggtg gtgaattcgt
      481 agaagccgtc ggtcacgatc aggcagcggc cgcccgcttt cgaattgccg aactcgcggc
      541 cgtcggagcg gaaattatag accggcttgc cgcccgggcc cgcccagctc caccgccgct
      601 ggaccagctc cgcgccccct tcccccgccc cggcgaagcg ccggcgcacg atcggagcgg
      661 tgtcggtgat tcggatctcc tcgcggggct ggaggttggg aaggccctcg gggaagctca
      721 ggaccattcc cagctgctcg aaggtcctgg cgatcgcgtc cggggcgacc ttgttgctgt
      781 agaggttgca catgcggccg cctcctagga ggccggaccc gactccgcca gcttgacagc
      841 gaatgccgct cccccgatat gcccccctcg acgcgcgacg gccccttcgt ctagcggtcc
      901 aggacgtcgc cctctcacgg cgaaaacacg ggttcgagtc ccgtaggggt caccagcaaa
      961 atcgattgcg gccgccgcgg gcagctgctg cgaacgccgg ctccggctta ccgacaagga
     1021 tgcgcggccg aggagctcga agacaggctg gcgtcggcca tcgagcctca ctaccctcgt
     1081 cggctccaga gcggggccgg ggtaggcgag cgccggaaga cgtcgatgga gcgaaaatga
     1141 ccgatcgggc gcttggaacg aagcatttgg aaatgcttca ggcggcgata agccgcctcg
     1201 ccggaaatgc cttcctgatc aaggggtgga gcatcgccct ggctacggcg gtcgcgggct
     1261 tcgcggtcaa ggacggcgct tcccgggtcg cactcgtcgg catcgtcccg atagctttgt
     1321 tctggtgcct ggactcatac tacctgtcgc tcgaacgcgc gtttcgcgcc ctgttcgaca
     1381 gcaccagggc tcaggtgctg gccgacctgc cgcccacgct cgacatgacg ccgagcctgg
     1441 cgccgctcgc cgtcgtcgcg aatgccttcc ggcccgcggt gatcctcgtc catctgcccg
     1501 cgcaaatcct cctgctcgcc gccgctttcg ccggcagttg aggccggctt gggggcacag
     1561 gggtcaccgc gacggcccgc ccgaaggttc gaacggcagg tcgagtagct ttcggaacag
     1621 ggaggggtaa gcgtaatcct cctccagcgc ccggggcaga tcgagccgcc gcttctcctt
     1681 ttcgtagatc aggcgggccg ctccgcccgt cgacgcgatc gggagcagcc gggccttgcc
     1741 gtgatctttg acgaacatcg cgaattcctg ctccacgcct tccatcccgc cgatgaagat
     1801 gccggcatcg aaggcggccg cggaaatcat cctttgccgc atggtgtgca ggctggagtc
     1861 ccgggtggct tgccgcggag tcagctcgta tctttcgaac tcggcattgt cggcgggcag
     1921 gacgtcggaa aaccaggcgg attggaagag gcggatccgg tccggcgctt tgatcccggc
     1981 attttccgca accacccgga tcaacggcgt gatcgcggga tggccgcccc agaacaaggc
     2041 cgcatggggc aggacgaccg tcgccagggc acggacgcag tcgcgaatcg ccaccagatc
     2101 ggcggtctcg taatatattg ggtcgcgcct cggatcgggg acgctggccg aaaggaagat
     2161 agccttcata gcaggccgcc gagcttggcc gccgtctcgg tgactcgaag ccctttcacg
     2221 ggaagatggt cgtcgagcca gtcgaggaac tggatccagg cgggaagcat gccgcgatgg
     2281 tcgtagatca ggaccgccgc gtcggcgcgc tcgccggcga ccgggaagcg tccggcggcg
     2341 tcgtgataga gctgggcgtc cggtacgccg accgccggga tcgccgccac gcgttggccg
     2401 ccaccgcgct gggcgaggat gaaacgctcc ggctggacgg ccgtgtgcac gccgattcgc
     2461 tcggcttctt cgcaaaactc cacgaccagg ttggtgtgcc gggcggcgat gcatcgggct
     2521 ctcaggacct cggtggcctg gatgatccgc tccgccgcgg catcggtgag ccggtcgccc
     2581 aggaagtcgg ccggatcgag atagagatgg gccgcgagct cggcctcccg aacgcgttcg
     2641 acgcccggcc agacgacccg aagcatgccc acgctcatcg ccgaagcctg ggccagctcc
     2701 tgccgggtcc agcggctggt gagaaaatcc gccgtgtcga gcacgatcat gacgtcgctg
     2761 tcgagcagcc ggtgccataa taccgactga aaatcgtcac cctttggcac cgagctcgtg
     2821 tcgaggaacg tatcgaagcc gcggtcgtcg aacgccaccc ggagctggtg cgcggcaacg
     2881 ctcgaatcct ttctgaaata gctcaggaaa agccgccgcc ttcgccggag cagggaaaga
     2941 ttttcgagcg cgatgttgat cgccgcggcg aagtcggggt cgtcccaggc gaccgccatc
     3001 ccgttgatcg gatgaagcgc cggaggcacc ttggcggcat agtcctccac cgcatcgacg
     3061 acaggaagga tggggacggc cattccgatg agctcgccga cggcggcctc gtcggtgggc
     3121 gcgccgccgc cgccgaaata gacggccacc gacggcgccg tcttgcgcag ggccgggatt
     3181 tcggaagcgc cgaggactcg aagggcgccc tcgagatcgc cgccgatttc ggcgaggcgt
     3241 tcggtcagct cggcgatgaa cacgtccttg tgcctcgcgc accccggccc gagaagtacg
     3301 atctgataga gggcatcctc tgccattgcc ttattccaca gaccggagat gccgacggtc
     3361 ggtcattgcg cgatccacgc cttcagcagt tcggcaccga aaacctggtg ctcccgtccg
     3421 aagcagggat caagcctgtt ccgccgacaa tctggtcctg gaggtagcac accgcgcatc
     3481 gccaataatc cggcagtcac gtaaataatg atacttagat cgcgagaatc gctggaatct
     3541 tcagcattgc cgcaggaagt taagtccgaa tcggttcttg tttcggataa gtcgagttgt
     3601 aatcccgtac ctgttggtta atgaatgtta attgggaggg ggcgtaaccg ggtatgctga
     3661 tcgcgctcaa ttcgccttcc gcacccggga cagggccggt actcgcctcg gtccacctcg
     3721 cgctcgacgc cgtcacgccg gcgacagtca ccgtcagtta cgagacgctg ccgggcaacc
     3781 agccgaagct ctacggcaat ttcctggcga tctggcagtc gacggtcatc ccctggtccg
     3841 ccccgccgct gaaacgccag gcgcttgccc ccggcgggca gagcggagca acgaccctga
     3901 gcggcctcgg gatacagcgg aagccctata tcgtcggtta tggcgtcggc gcggagatca
     3961 ccagcatcgc cgcgagcctg gcgctccatc cggaccggga ggcggtggtc ctggcgacga
     4021 ccatcgaggt gcccgcgatc actccggaca gcctcgtcat ccattatcgc accctgcccg
     4081 gctatcgccc gctgaccgcg ggcaactgga tcggcctctg gcagggacaa gcctctccct
     4141 tctactccgg cgacccgatc gcccgggcag ttccagacca cgatttctcg gagggctaca
     4201 tcgcgatgga cggcctcgtc ctgacgttcg ggacgaccta cactttggtc tacttcgcgg
     4261 gcgccgggaa aaccgaagcc gccgccatcc ttacctttac caccgcctcg agaagctgac
     4321 gaaacctcag atagaaaggg agatttgcca tggtcactcg tacccgatcc caggacggcg
     4381 caaaagc
//
LOCUS       DASWNU010000475         5074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_70259, whole genome shotgun sequence.
ACCESSION   DASWNU010000475 DASWNU010000000
VERSION     DASWNU010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70259"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGB57_09315"
     CDS             <1..399
                     /locus_tag="VGB57_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEY0131591.1"
                     /translation="IGHDAPGGTSRQVVRIILADRAAGNYLGKVAVARDAQKSDGSQS
                     VKAMLVEAGPTANLKPELEIFADDVKCAHGATVGELDRDALFYLASRGLPEADARALL
                     TRAFVADAIDRAGEAAVREALHEDAEAWLG"
     gene            399..1628
                     /locus_tag="VGB57_09320"
     CDS             399..1628
                     /locus_tag="VGB57_09320"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY0131592.1"
                     /translation="MDIAVSARPLDHVADFPAIPEGWSYLDTAATAQKPRAVIEAIER
                     GYSETYATVHRGVYRRSSEMTLAFEAARRRVAGFIGAPSPNEIVFVRGATEGINLVAQ
                     SAFENRDSPYFLRPGDRIMLSMLEHHSNIVPWQLVAARTGAEVDVAPLTEDGRIDLDA
                     MSAMLTERHKIVALAHVSNVLGSVLDVPRAAKLAHSAGALLLLDGCQAVPRMPVDVAG
                     LGCDFYVFSGHKLYGPTGVGVLWGRTELLAAMPPWQGGGAMIDKVSFEGTTFAPPPAR
                     FEAGTPHIVGVLGLEAAVGYVERIGPEAIGAHERALVREAREALSSLNSVRLFGPDDS
                     AGIVSFAFEGVHPHDIGTILDESRVAIRAGHHCAQPLMAHLGVDATARASFGVYNGPR
                     DIAALVRGLERVRRIFG"
     gene            1625..2068
                     /locus_tag="VGB57_09325"
     CDS             1625..2068
                     /locus_tag="VGB57_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEY0131593.1"
                     /translation="MNEERRIEVEEVDAATSPPRARVSDAFERKRDYLEGFLSKQKPA
                     EPEGQLYESVIEALKDIYDPEIPVNIYDLGLIYDVEITADNHALVKMTLTTPNCPVAE
                     SMPGEVELRVGSVPGIGHAEVELVWDPPWDPQRMSDEAKLELGML"
     gene            2065..2430
                     /locus_tag="VGB57_09330"
     CDS             2065..2430
                     /locus_tag="VGB57_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0131594.1"
                     /translation="MSETATAPRRARRAALTLTPAAEARVAALMARAPDGTLGVKLST
                     PRRGCSGLAYSVDYVAEASPVDERIDTPAGPFFIDGASILYLIGSTMDWQEDDFAAGF
                     VFQNPNAKGQCGCGESFTV"
     gene            2623..2892
                     /locus_tag="VGB57_09335"
     CDS             2623..2892
                     /locus_tag="VGB57_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEY0131595.1"
                     /translation="MNDRDFLLQVQGYGLMTAEVAYYMPDHPGLIQLFVWQQYDVAPR
                     FPVLHRFLDHWRTDIEAVLHSVRIAHQHLIRPAEWRAVDGVISIN"
     gene            3016..3984
                     /locus_tag="VGB57_09340"
     CDS             3016..3984
                     /locus_tag="VGB57_09340"
                     /inference="COORDINATES: protein motif:HMM:NF025772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4424 domain-containing protein"
                     /protein_id="HEY0131596.1"
                     /translation="MKAIVPVLLAALALAAPAAANDSTAEHAAGGLVLTRSGDIDMVS
                     EDLFVSAGEVRVRYVFRNRGPREIRTIVAFPMPDRDLGEEREMDVAYPSGFETRVDGR
                     RVTMKVERKAMVGGVDRTALLAALGVATDAGSEALDRLKPADQERLVKLGVAGRDEYD
                     AGKGWERHLAPAWTVKESWFWEQVFPAGRDLVVEHRYVPGTGGSVDAALGFPEFRASP
                     EGRAMIRDYCVEPAFLAGLDRLRRRSPATPEQRIGYILTTGGNWRSPIGDFRLVVDKG
                     APANLVSFCGDGVRKISPTSFEMRKKNWRPDRDLRILIVQAQPPGE"
     gene            3987..4379
                     /locus_tag="VGB57_09345"
     CDS             3987..4379
                     /locus_tag="VGB57_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEY0131597.1"
                     /translation="MIGQAAHPDALARLMEPVATALEIFGVLVIVAGIAWATARFLLR
                     DLRSGDAVKAYERYRANLGRGILLGLEILVGADIVATVTAPLTFRSVGLLGLIVLIRT
                     FLSFSLETEIEGRWPWRRRGTPGEADSR"
     gene            complement(4481..>5074)
                     /locus_tag="VGB57_09350"
     CDS             complement(4481..>5074)
                     /locus_tag="VGB57_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HEY0131598.1"
                     /translation="FSVAADRRVGRARLSLGATRLDEQSTVFGARFSPLIGVGGAASW
                     FADAGATVALGRGWDASIGYRLGWTDVPAAAGLAQGGRLATDAWSVDLARSGLLRSGD
                     RFAVRLMQPLRVRSGGLDLSVPVSYSYDTLTAAYESRFFNLAPTGREIDIEAAYGFDL
                     FAGAGHLGANAFARRHPGNLAAMDADLGAAIRFTLGF"
BASE COUNT          811 a         1759 c         1717 g          787 t
ORIGIN      
        1 atcggccatg acgcgccggg cgggacgagc cggcaggtcg tgcgcataat tctagcggat
       61 cgcgcggcgg gcaattatct cggcaaggta gcggtcgcgc gcgacgcaca gaagagcgac
      121 ggctcgcaat cggtgaaggc aatgctggtc gaggcggggc cgaccgcgaa cctaaagccc
      181 gagctggaaa tcttcgccga cgacgtcaaa tgcgctcacg gcgccacggt cggcgagctc
      241 gaccgggacg cccttttcta cctggcgagc cggggcttgc ccgaggcgga tgccagggcg
      301 ctgctcaccc gagccttcgt cgccgacgcg atcgaccgcg ccggcgaagc ggcggtgcgc
      361 gaggcgctgc acgaggatgc cgaggcctgg ctgggatgat ggacatcgcc gtctccgccc
      421 gccccctcga ccatgtcgcc gacttcccgg cgatccccga gggctggtcc tatctcgaca
      481 cggccgcgac ggcgcagaag ccgagggcgg tgatcgaggc gatcgagcgc ggctattccg
      541 aaacctatgc gaccgtccat cgcggcgtct accggcgctc gtcggaaatg accctggcgt
      601 tcgaggcggc gcggcgccgg gtggccggct tcatcggagc gccttcgccc aacgagatag
      661 tattcgtccg cggtgccacc gaggggatca acctcgtcgc ccaaagcgcc ttcgaaaaca
      721 gggacagtcc ctattttctg aggccggggg accggatcat gctctcgatg ctcgagcatc
      781 attcgaacat cgtcccgtgg cagctcgtcg ccgcgaggac cggcgcggag gtcgacgtcg
      841 ctccgcttac cgaggacggc cgaatcgacc tcgacgctat gtcggcgatg ctgaccgagc
      901 ggcacaagat cgtcgccctc gcccatgtct cgaacgtgct cggctccgtc ctcgacgtac
      961 cgcgcgccgc gaagctggcg cattccgccg gcgcactgct ccttctcgac ggctgccagg
     1021 cggttccgcg gatgccggtc gacgtcgccg ggctcggctg cgacttctac gtcttttccg
     1081 gccacaagct ctacggcccg accggggtcg gagtcctgtg gggccggacc gagctgctcg
     1141 cggccatgcc gccctggcag ggcggcggag cgatgatcga caaggtcagc ttcgagggca
     1201 cgactttcgc cccgccgccg gcccgcttcg aggccgggac cccccatatc gtcggagtgc
     1261 tcgggctcga ggcggcggtc ggctatgtcg agcggatcgg cccggaagcg atcggcgccc
     1321 atgaacgggc gctggtgcgc gaggctcgcg aggccctgtc gtcgctgaac agcgtccggc
     1381 tgttcgggcc ggacgattcc gccggaatcg tcagcttcgc attcgaaggg gtgcatccgc
     1441 acgacatcgg caccatattg gacgagagcc gggtggcgat ccgcgccggc catcattgcg
     1501 cccagccgct gatggcccat ctgggcgtgg acgcgacggc acgggcgagc ttcggagtct
     1561 ataacggccc acgcgatata gcggcgctgg ttcggggcct ggaacgggtg agaaggatct
     1621 tcgggtgaac gaggagcgca ggatcgaggt cgaggaggtg gacgcggcga cgtcccctcc
     1681 cagggcgcgc gtctccgatg cgttcgagcg caagcgcgac tatctcgaag gcttcctttc
     1741 gaagcagaag ccggccgagc cggaaggcca gctgtacgag agcgtgatcg aggcgctgaa
     1801 ggacatttac gaccccgaaa tcccggtcaa catctacgat ctcggcctca tctacgatgt
     1861 cgagatcacc gcggacaatc acgcgctcgt caagatgacc ctgaccactc cgaattgccc
     1921 ggtcgccgaa tccatgcccg gcgaggtcga gcttcgggtc ggctcggttc ccgggatcgg
     1981 ccatgccgag gtcgagctgg tctgggaccc gccctgggac ccgcagagga tgagcgacga
     2041 agccaagctc gaactgggaa tgctatgagc gagaccgcca ccgccccccg ccgcgcccgc
     2101 cgcgccgcgc ttaccctcac tccggccgcc gaggctcggg tcgcggcctt gatggcgcgg
     2161 gcgccggacg gcacgctcgg ggtcaagctg tcgactcccc ggcgcggctg ttccggcctc
     2221 gcttattcgg tcgactatgt cgccgaggcc agcccggtcg acgagcgcat cgacaccccg
     2281 gccggcccct tcttcatcga cggcgcctcg atcctctacc tgatcggatc gacgatggac
     2341 tggcaggagg acgatttcgc cgccggcttc gtcttccaga atcccaacgc caagggccag
     2401 tgcggctgcg gcgagagctt caccgtctga gctcctgcgt catcccgggc ttgacccggg
     2461 atccatgaac acggcggttc acgtcacatt cggcggtgtt catggattcc ggctttcgcc
     2521 ggaatgacga gagcgggtgc ggacggcggc atccgctaac atggttagtt tgccgttaag
     2581 catgttgcgc tatgctgccc ccaacaaggt tcggagggcg cgatgaacga ccgcgatttc
     2641 ctgctccagg tccagggcta cggcctgatg acggccgagg tcgcctatta catgccggac
     2701 catcccgggc tcatccagct cttcgtctgg cagcaatatg acgtcgcccc gcgcttcccg
     2761 gtcctccacc gcttcctcga ccactggcgc accgacatcg aagcggtcct ccactccgtc
     2821 cggatcgccc accagcatct catccgcccc gccgaatggc gcgccgtgga cggcgtgatc
     2881 tcgatcaact gacgccgaag gcgtcatccc ggcgaaggcc gggatcccgg cccatctggc
     2941 agagcctttt tccgcgctga ggccccggct ttcgccgggg tgacggctgc gctgccgcgc
     3001 ggtaagctcc ggcccgtgaa ggcaatcgtc cccgtcctcc tcgccgccct cgcgctcgcc
     3061 gcgcccgccg ccgcgaacga cagcaccgcc gaacatgcgg cgggcgggct cgttcttacc
     3121 cgaagcgggg acatcgacat ggtctccgaa gacctgttcg tctctgcggg cgaggttcgg
     3181 gtgcgctatg tcttccgcaa ccgtggcccg cgcgaaatcc ggacgatcgt cgccttcccg
     3241 atgccggacc gggacctcgg cgaggaacgg gagatggacg tcgcctaccc ctccggcttc
     3301 gagacccggg tggacgggcg gcgggtgacg atgaaggtgg agcgcaaggc gatggtcggc
     3361 ggggtcgacc gcaccgcgct tctggccgcg ctcggagtcg cgaccgacgc tgggagcgag
     3421 gcgctggacc ggctgaagcc ggctgaccag gaaaggctgg tcaagctcgg cgtcgccggg
     3481 cgcgacgaat atgatgccgg caaggggtgg gagcgccacc tcgccccggc ctggacggtg
     3541 aaggagagct ggttctggga gcaggtcttc ccggccggcc gcgacctcgt cgtcgagcat
     3601 cgctacgtgc cgggcaccgg cggcagcgtc gacgcggcgc tcggctttcc cgaattccgc
     3661 gccagccccg aaggccgggc catgatccgc gactattgcg tcgagcccgc cttcctcgcc
     3721 ggcctcgaca ggctgcgccg ccgaagcccg gccactcccg agcagcggat cggctacatc
     3781 ctcaccaccg gcggcaactg gcgctcgccg atcggcgatt tccggctggt ggtcgacaag
     3841 ggcgcaccgg ccaacctcgt cagcttctgc ggcgacggcg tccgcaagat ctctccgaca
     3901 agcttcgaga tgcggaagaa gaactggcgt cccgaccggg atctcaggat cctcatcgtc
     3961 caggcccagc ctccgggaga atgaacatga tcggacaggc cgctcacccc gacgccctcg
     4021 cccgactgat ggagccggtc gcgaccgcgc tcgaaatatt cggggtcctc gtcatcgtcg
     4081 ccggaatcgc ctgggcgacg gcccgcttcc tgcttcgcga ccttcgctcg ggcgacgcgg
     4141 tgaaggccta tgagcgctac cgcgccaatc tcggccgggg aatcctgctc gggctcgaga
     4201 tactggtcgg cgccgacatc gtcgccaccg tcacagcgcc gttgaccttc cggagcgtcg
     4261 gcctgctcgg cctcatcgtc ctcatccgaa ccttcctcag cttctcgctc gagaccgaga
     4321 tcgaaggccg ctggccctgg cgccgccgag gaactccggg agaggcggat tcgcgctgag
     4381 gtcgcccgtc attgcgagcg aagcgaagca atccagcggc tcaggcgccg gacactggat
     4441 tgcttcgtcg ctgcgctcct cgcaatgacg gttcgcaggt ctaaaagccc agggtaaacc
     4501 tgatcgccgc ccccagatcg gcgtccatcg ccgccaaatt gccgggatgg cggcgggcga
     4561 aggcgttcgc gccgaggtgg ccggcgcccg cgaacaggtc gaagccataa gccgcctcga
     4621 tgtcgatctc gcggcccgtg ggcgcgaggt tgaagaagcg gctttcatag gcggcggtca
     4681 gcgtatcgta gctgtaggag acgggcacgc tgaggtccag gccgccggaa cggactcgaa
     4741 gcggctgcat cagccgaacc gcgaagcggt cgccggagcg caggaggccg ctcctggcga
     4801 ggtcgaccga ccaggcgtcg gtggcgaggc gcccgccctg ggcaaggccc gcggcggcgg
     4861 gaacatcggt ccagcccagg cgatagccta tcgacgcgtc ccagccgcgg cccagcgcca
     4921 cggtcgctcc ggcatcggcg aaccagctgg ccgctccgcc gactccgatg agcggggaga
     4981 aacgggctcc gaacacggtc gactgctcgt cgaggcgggt ggcgcccagg ctcagcctcg
     5041 cccggcccac ccggcggtcg gctgcgaccg aaaa
//
LOCUS       DASWNU010000476         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_123138, whole genome shotgun sequence.
ACCESSION   DASWNU010000476 DASWNU010000000
VERSION     DASWNU010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123138"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VGB57_09355"
     CDS             <1..511
                     /locus_tag="VGB57_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0131599.1"
                     /translation="AQFESFVHPAVIIATVPLAVAGGGIGLAVMGQTLNLYSQVGLVM
                     LVGLAAKNGILIVEFANQLRDQGRDIAEAIREASARRLRPILMTSIATVAGAVPLMLA
                     SGAGAAARTAIGVVIVFGVSISTLITLFLIPLLYSKLARYTGSPLAVTRKLEEQLSHP
                     AGAQPAPAE"
     gene            640..1194
                     /locus_tag="VGB57_09360"
     CDS             640..1194
                     /locus_tag="VGB57_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY0131600.1"
                     /translation="MTALASRSQLRMSFLRYALFTVPLVLLLGTISGRIANSGYGNVW
                     FDALQKPAIMPPGWMFGAAWTILYILLGFAVALILHARGARGRGLALSLFVLQLLLNY
                     AWSPIFFAYHEVGAAFWTILAMIVISAVTAFLFWRIRRSAGLLMLPYLAWLCFAATLT
                     WQIGVLNPGAAELAPGGSTTDIAL"
     gene            1218..1517
                     /locus_tag="VGB57_09365"
     CDS             1218..1517
                     /locus_tag="VGB57_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY0131601.1"
                     /translation="MQSENRLFDDFVKVMNGAAGTIAGMGREAENAFRDRMRDWVGGL
                     DMVSREEFDAMKAIAIAAREESVALKRRVEALEAAAAATPKREPGSEAGTGPVGQ"
     gene            1612..2118
                     /locus_tag="VGB57_09370"
     CDS             1612..2118
                     /locus_tag="VGB57_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY0131602.1"
                     /translation="MNVDEFEMEREPGGPIDMLEHYFSAHGWTAERGEDEIVANFQGS
                     WAQYELRAVWRDEDHVLQFLALPDIRVAQDKRAAVYETIGLINEQLWIGHFELWASSG
                     LVLFRHATLLEGEEGGVLTLQHAETLVEAAIEECERFYPVFQFVLWAGKTPQEAIAAA
                     LIETQGEA"
     gene            2236..3051
                     /locus_tag="VGB57_09375"
     CDS             2236..3051
                     /locus_tag="VGB57_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEY0131603.1"
                     /translation="MTTLTLPGPLFLIGCGNMAGAMLEGWLAAGADPRLITVVRPSGR
                     APAPGIRTLTALPEDEVPALVLLGVKPQKLAEVAPAVAPALERETILVSILAGTTLAA
                     LRGFFPVPRTIVKAMPNLPVSLRKGVIELHADGAGCEAGKAEVERLMRALGHVEWFDD
                     EALFAVASALTAAAPAFLYRFLDALAAAGEELGLPAEQSARLAATMAEGASALAIGSG
                     AAPRDLARRVASPGGTTEAGLAVLDSEEGLKALVLRTLDASRRRGQEMAGLTS"
     gene            3163..3450
                     /locus_tag="VGB57_09380"
     CDS             3163..3450
                     /locus_tag="VGB57_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131604.1"
                     /translation="MRMFVLAATLAAAVALPATAASARHNDHYGYNNRGEVRQELREC
                     RRELRRADSRWEYRREQRECRRELREARRDQYRDGRRYGYRHDDRRYRYRY"
BASE COUNT          548 a         1296 c         1206 g          589 t
ORIGIN      
        1 cgcccagttc gaaagcttcg tccatccggc ggtcatcatc gccaccgtgc cgctggcagt
       61 ggcgggcggc ggcatcggcc tggccgtgat gggccagacg ctcaacctct acagccaagt
      121 cggcctggtc atgctggtcg ggctcgccgc caagaacggg atcctcatcg tcgagttcgc
      181 caaccagctt cgcgaccagg gccgcgacat cgccgaggcg atacgcgagg cctcggcccg
      241 gcgcctgcgc ccgatcctga tgacctcgat cgccaccgtc gccggcgccg tgccgctgat
      301 gctggccagc ggggccggcg ccgccgcccg gaccgcgatc ggagtcgtca tcgtcttcgg
      361 agtctcgatc tcgaccctca tcacgctctt cctgatcccg ctcctctatt cgaagctcgc
      421 ccgctataca ggctcgccgc tggcggtgac gaggaagctc gaggagcaat tgagccatcc
      481 ggccggcgct cagccggcgc ccgcggaatg agtcggcgtc atcccggcga aggctgggat
      541 cccagcgggt gaaaggcgcc tgcgttcttc tcccgctggg gccccagcct gcgcaggggc
      601 gacgggatcg ggcccgggtg gctcattgag ggagtcgaca tgaccgcact ggcctccagg
      661 tcccagctca ggatgtcgtt cctgcgctac gctttgttca ccgtgccgct ggtgctcctg
      721 ctcgggacca tttccgggcg gatcgccaat tccggctacg gcaacgtctg gttcgacgca
      781 ctccagaagc cggcgatcat gccgcccggt tggatgttcg gcgccgcctg gacgatcctc
      841 tacatcctcc tcggcttcgc ggtggcgctg atcctccacg cccgcggcgc ccgcggccgc
      901 ggcctcgcgc tcagcctgtt cgtcctccag ctcctcctca actacgcctg gtcgcccatc
      961 ttcttcgcct atcacgaggt cggcgccgcc ttctggacga tcctcgcgat gatcgtcatc
     1021 tcggccgtca ccgccttcct gttctggcgg atccgacgct ccgcgggcct tctgatgctg
     1081 ccctatctcg cctggctttg cttcgcggcg acgctcacct ggcagatcgg agtcctgaac
     1141 ccgggggcgg ccgagcttgc gccgggcggc tcgactaccg atatcgccct ctaaggcact
     1201 cgaaaggagg acttgcgatg cagtccgaaa accgtctgtt cgacgatttc gtcaaggtca
     1261 tgaacggcgc cgccggcacc atcgcgggaa tgggccgcga ggccgagaac gcgttccgtg
     1321 atcggatgcg cgactgggtc ggcggcctcg acatggtctc gcgcgaggag ttcgacgcga
     1381 tgaaggcgat cgccatcgcc gcccgagagg agagcgtcgc gctgaagcgc cgggtcgagg
     1441 cgctcgaggc cgcggcggcg gcaacgccga agcgcgagcc gggctcagag gccggtacgg
     1501 ggcctgtggg ccaataatcc acaacatatt gtggtcgcct gatctgcgtt cttgcgccgg
     1561 cgttcgcgcg caggcagttt cggggaaaca gggcggccgg aaagctgagt gatgaacgtg
     1621 gacgaattcg agatggagcg cgagccgggc ggcccgatcg acatgctcga acattatttc
     1681 agcgcgcacg gctggaccgc cgagcgcggc gaggacgaga tcgtcgccaa tttccagggc
     1741 agctgggcgc aatatgagct tcgcgcggtc tggcgggacg aggatcatgt cctccagttc
     1801 ctcgcccttc ccgacattcg cgtggcgcag gacaagcggg cggccgtgta cgagacgatc
     1861 ggcctcatca acgagcaatt atggatcggc catttcgagc tttgggcctc gtccggcctc
     1921 gtcctcttcc gccacgccac cctgctcgaa ggcgaggaag gcggagtcct caccctccag
     1981 cacgccgaga ccctggtcga ggcggcgatc gaggaatgcg agcgcttcta ccccgtcttc
     2041 cagttcgtcc tctgggccgg caagacgccc caggaagcca tcgccgcagc gctcatcgag
     2101 acccagggcg aggcgtagcg gaaggcggag ggttctccgt cattgcgagc gtagcgaagc
     2161 aatccattgc cgcggccggg cgaaaccgct gaagctggat tgcttcgtcg ctccgctcct
     2221 cgcaatgacg gagccatgac cactctcacc ctccccggac cgctcttcct gatcggctgc
     2281 ggcaacatgg ccggggcgat gctcgaaggc tggctcgccg ccggggccga ccctcgactg
     2341 atcaccgtcg tccgccccag cggccgcgcc cccgcgcccg gaatccgaac cctcaccgcc
     2401 ttgccggagg acgaggttcc ggcgctggtc ctgctcggag tgaagcccca gaagctcgcg
     2461 gaggtcgccc ccgccgtcgc gccggcgctc gagcgggaga cgatcctcgt ctcgatcctc
     2521 gcgggcacca ccctggccgc cctgcgcggc ttcttcccgg tcccgcggac gatcgtcaaa
     2581 gccatgccga accttcccgt cagcctgcgc aagggggtca tcgagctgca tgccgatggc
     2641 gccggctgcg aggccggcaa ggccgaggtc gagcggctga tgcgcgcgct cggccatgtc
     2701 gaatggttcg acgacgaggc gctcttcgcc gtcgccagcg ccctcaccgc cgccgccccg
     2761 gccttcctct accgcttcct cgatgcgctt gcggccgcag gcgaggagct gggcctgccc
     2821 gcggagcagt cggcacgcct cgccgccacg atggccgaag gggcgagcgc tctcgcgatc
     2881 gggtccggcg cggctccgag ggatctcgcc cgccgggtcg ccagccccgg cggcacgacc
     2941 gaagcggggc tcgccgtgct ggactccgaa gaggggctca aggcgctggt gcttcggacg
     3001 ctggacgcct cgcgccggcg cggccaggaa atggccggcc tcacatccta aacccccgat
     3061 gaacgcggcg tagtttgggg ttcatgcaac gcggcatatc ttccgctcgt ttccaccagc
     3121 gcccgcgatc cgcgcgggcg ctccagtcga ggagagacga gtatgcgtat gtttgtactg
     3181 gccgcgaccc tcgcggccgc ggtcgcgctt ccggcgacgg ccgcttcggc gcgtcacaac
     3241 gaccattatg gctacaacaa tcgcggcgaa gtccgccagg agctgcgcga gtgccggcgc
     3301 gaattgcgcc gcgccgacag ccgctgggag tatcggcgcg agcagcgcga gtgccgccgg
     3361 gagctgcgcg aagcgcgccg cgaccagtat cgcgacggcc gccgctacgg ctatcgccac
     3421 gacgatcgac gctaccgtta ccgctactag gcggcgaatg tcccaccggg cggcgtgatg
     3481 ccgcccggtg gacgcgcgaa ggcccgatgc cctcgaccgt catccgcacg atcgaatatc
     3541 gcccggagat gggcgagctg gaagtgacgt tcaccaccgg acgccgctac ctgttccacg
     3601 acgtcccggc cgaagccgcc gaggccttcg ggcaggcac
//
LOCUS       DASWNU010000477         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_407738, whole genome shotgun sequence.
ACCESSION   DASWNU010000477 DASWNU010000000
VERSION     DASWNU010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407738"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..821
                     /locus_tag="VGB57_09385"
     CDS             270..821
                     /locus_tag="VGB57_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY0131605.1"
                     /translation="MSRFTPIAAALALAAAPAAFAAARTPMVGGAAMYPSRNIVENAA
                     QSKSHTTLVAAVKAAGLVDTLAGAGPFTVFAPTNAAFGKLPAGTVDTLLKPENKPALQ
                     SVLTYHVVPGRVSAAQLTAKIKAGGGMAKLATVQGGTLTARLVGGKVVLTDEKGGRAT
                     VTQADVFQSNGVIHVTDAVSLPR"
     gene            complement(989..1816)
                     /locus_tag="VGB57_09390"
     CDS             complement(989..1816)
                     /locus_tag="VGB57_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0131606.1"
                     /translation="MSAAVFFAILFAAALHAGWNALVKTGLDRFSSILFLGIVQGGIA
                     LLLLPFFGLPARAAWPWVLAGSALHSGYKLALIRAYGHGDLSQIYPLSRGTAPLIVAV
                     AGALFLGEKMDLARSAAVVAIAAGIMMMAGRGGLTRAGLFWALVTAGFTAAYTLADGV
                     GARIAGSPSAFILAMIVLDGLIMLAWGLAARGRAGIAAMLPEWRPGVAAGAMSLGAYW
                     IAIWAFTQAPLAMVAALRETSVLFAMLIAAVLLKERPTPRRWAAAALIVAGVVMMRV"
BASE COUNT          355 a          792 c          660 g          279 t
ORIGIN      
        1 cggggctgta gcctgcctcc ccatcgccag atggggaggg ggaccacccg aagggtggtg
       61 gaggggtaac tggcgcggaa gccgaagatt tggccaggag cctctgaagc cgagccgact
      121 acccctccac catgcttcgc atggtccccc tccccacggc ttcgccgcag ggaggcagag
      181 aaaggctcct acatcactag gtccgtatcg ctcctctacc cggcggcgcc tagtgggctg
      241 gacctccccc gtttccaatg aggtccccca tgtccagatt caccccgatc gccgccgccc
      301 tcgccctggc cgccgcgccg gccgccttcg ccgccgccag gactccgatg gtcggcggcg
      361 ccgccatgta tccgtcgcgc aacatcgtcg agaacgccgc ccagtcgaag tcgcacacca
      421 ccctcgtcgc cgcggtcaag gccgccggcc tcgtcgacac gctcgccggc gccggtccgt
      481 tcaccgtctt cgcccccacc aacgccgcct tcggcaagct ccccgccggc accgtcgaca
      541 ccctgctcaa gcccgagaac aagccggcgc tccagtccgt cctcacctat catgtcgtcc
      601 cggggcgggt cagcgccgcc cagctgacgg ccaagatcaa ggcgggcggc ggaatggcga
      661 agctcgccac cgtccagggc ggcaccctca ccgcccggct ggtcggcggc aaggtcgtcc
      721 tcaccgacga gaaaggcggc cgcgccaccg tcacccaggc cgacgtcttc cagtccaacg
      781 gcgtcatcca cgtcaccgac gccgtctccc tcccgcgctg aaccctgcgc gcgggacggg
      841 ccgcccccgc tccgccccaa aggggtgggg gcggccctac ctcattcgtc attccggaag
      901 gccggaatcc atgaacaccg aagtcgcctt gttgcctcga tccgtgttca tggatcccgg
      961 atcaagtccg ggatgacggc ttgcggcgtc acacccgcat catcaccacc cccgcgacga
     1021 tcagggcggc ggccgcccag cgccgcgggg tcggcctctc cttgagcagg acggccgcga
     1081 tcagcatcgc gaacaggacg ctcgtctccc tcagggccgc gaccatcgcc agcggcgcct
     1141 gggtgaaggc ccagatcgcg atccaatagg cgcccagcga catcgccccg gccgccacgc
     1201 ccggccgcca ttccggaagc atcgccgcga tcccggcccg tccgcgggcg gcgaggcccc
     1261 aggcgagcat gatcaggccg tcgaggacga tcatcgccag gatgaaggcg gaaggcgagc
     1321 cggcgatcct cgcaccgact ccgtcggcca gggtgtaggc ggcggtgaag ccggccgtga
     1381 ccagagccca gaagaggccg gcgcgggtga gcccgccccg ccccgccatc atcatgattc
     1441 ccgcagcgat cgcgaccacc gccgcgctcc tcgccaggtc catcttctcg ccgaggaaca
     1501 atgctccggc gacggccacg atcagaggcg cggtgccgcg cgagagcgga tagatctggc
     1561 tgagatcgcc atggccgtag gcgcggatga gggcgagctt atagcctgag tgaagcgccg
     1621 accccgccag cacccacggc caggcggccc gcgccggcag cccgaagaaa ggcagaagga
     1681 ggagcgcgat tcctccctgc acgatgccga ggaacaggat cgaggagaag cggtcgagcc
     1741 cggtcttgac gagggcgttc caccccgcgt ggagcgcggc ggcgaacagg atcgcgaaga
     1801 aaacggcggc gctcattcga ccgtcatccc ggactggatc cgggatccat gaacacggat
     1861 cgcatcatat caggcacccc cggtgttcat ggattccagc ttccgccgga atgacggtaa
     1921 tagcccttta gccggccggt gctacaaagc gccgccatgg cgagccccac cgtccagctc
     1981 ttctacaacc gcacctccgg cggccattgc ccgcgccggc tcgcggcgct tcgggccggg
     2041 ttcgaatcga aaggcgcgaa ggtcctgctc tccgaatgcg gcccgg
//
LOCUS       DASWNU010000478         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_511710, whole genome shotgun sequence.
ACCESSION   DASWNU010000478 DASWNU010000000
VERSION     DASWNU010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511710"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1511
                     /locus_tag="VGB57_09395"
     CDS             111..1511
                     /locus_tag="VGB57_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0131607.1"
                     /translation="MADGETALLLARAQFAFTVSFHFIFPAFSIGLASYLAVLEGLWL
                     WTGKGRFLTLFRYWLKIFAIAFAMGVVSGIVMSYQFGTNWSVFSDRAGPIVGPLMAYE
                     VLTAFFLEAGFLGVMLFGLERVGKKLHFLATLMVAAGTAMSAFWILSVNSWMQTPTGW
                     AMNAQGQFVPAGSWLDIIFNPSFPYRLVHTVLAAYLTTALVVGAVGAWHLIKGRDNKA
                     ARTSFSMAMWMAALVAPVQIFVGDLHGLNTLEHQPAKIMAMEGHFESYEKGAPLILIG
                     HPDRAAEKTRVLIEVPKLSSIILKHDPDAPLAGLKTIPKSDRPPAEIVFWSFRIMVGL
                     GLLMFALGLWSLLARLRKRLYDWRWLHRFAVAMGPAGFVAVIAGWVTTEVGRQPWVIY
                     GLLRTKDAVSPIASPGVTGSLIAFVLVYFAVFGAGTMYILKLMAKAPEEGEGEEPRTP
                     IRTAGITPAAGERRGG"
     gene            1684..2682
                     /gene="cydB"
                     /locus_tag="VGB57_09400"
     CDS             1684..2682
                     /gene="cydB"
                     /locus_tag="VGB57_09400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0131608.1"
                     /translation="MVDLTIVWAFLIAFAVAAYVVMDGFDLGIGILFPLFPVGKERDG
                     AMNAIAPVWDGNETWLVLGGGGLLAAFPLAYAIILPALYAPLIVMLLGLVFRGVAFEF
                     RWRDAGHRRWWDSGFFLGSLAATFAQGVTLGALIQGITVEGRAYAGGWWEWLSPFSLL
                     TGASLVAGYALLGAGWLIWKSEGPLQDRARRRARLLLPALLAAIGAVSLYTPFVEPKY
                     FERWFQMPGLFVAVPMPLLVAAAAFFGWRSLGRGRDWAPFVWSLALFGLTLAGLAISI
                     WPDVIPGRVSIWQAASPHSSQLFMLVGALVLIPLILAYTAWSYWVFRGKVGEEGYH"
     gene            2684..2779
                     /locus_tag="VGB57_09405"
     CDS             2684..2779
                     /locus_tag="VGB57_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HEY0131609.1"
                     /translation="MAKRLAWFVGLWAAGVASVASVAYLLRWWLL"
     gene            2788..3135
                     /locus_tag="VGB57_09410"
     CDS             2788..3135
                     /locus_tag="VGB57_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY0131610.1"
                     /translation="MLVAGAALAGLGVALGAFGAHALKASLPPQALGWWDIAVQYQMW
                     HALGLILVGVLPQPRLGLPAALLGGGTLLFSGSLYAMALTGERWLGAVTPLGGAAMIA
                     GWALLAWRLRSAA"
     gene            complement(3149..3670)
                     /locus_tag="VGB57_09415"
     CDS             complement(3149..3670)
                     /locus_tag="VGB57_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0131611.1"
                     /translation="MAGEAGGDDGAASILIRAFETADSAAVDAVALEAFEQYRGVYDD
                     WRTLEQAVGRTSAMAADAELLIAEVDGRVAGAVAYCPPFSEPRADFFESRWPLIRMLV
                     VAPSARGRGLGRSLTDACIERARRDGAASIALHTSPAMEVALALYLRMGFRLERRVPD
                     RFGVPYAVYLLDL"
     gene            complement(3794..>4421)
                     /locus_tag="VGB57_09420"
     CDS             complement(3794..>4421)
                     /locus_tag="VGB57_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131612.1"
                     /translation="LAAFALFAGIYALGWFGLLIATRFVMPAVLMLQIGLGALFLALV
                     DDWRTLPRSAQLGLFTFALLCVGVSVVLTRAQLMAEQEKFAREGNAYTAALALTRDIP
                     DSRPVAAYDVSAWPIVATGQRVVSVPWPEPMIDGLAERQEAAERLFDPTLSRDERLRL
                     ARRWGATTLIMDKKGPLRRKMPKFMLERLQAQSVRHAEAGRFIRFDLE"
BASE COUNT          601 a         1473 c         1554 g          793 t
ORIGIN      
        1 cgcgggttcc cctccccatc ttcgatgggg aggcaaagag atgcagcgaa cgcatctgca
       61 tcagcctatt tcgcagcgcg gcggccctcg gcttatgcta tgcgcgcgcc atggccgacg
      121 gcgaaaccgc cctgctcctc gcccgcgcgc aattcgcctt cacggtctcg ttccatttca
      181 tcttcccggc tttctcgatc gggctggcga gctatctcgc cgtactcgaa gggctgtggc
      241 tgtggaccgg gaaggggcgc ttcctcaccc tgttccgcta ctggctgaag atcttcgcca
      301 tcgccttcgc gatgggcgtc gtctcgggga tcgtcatgtc ctaccagttc gggaccaact
      361 ggtcggtctt ctccgaccgg gccgggccga tcgtcggacc gctcatggcc tatgaggtgc
      421 tgaccgcctt cttcctcgag gcggggttcc tgggggtgat gctgttcgga ctggagcggg
      481 tcggcaagaa gctccacttc ctggcgaccc tgatggtggc ggcggggacg gcgatgtccg
      541 ctttctggat cctgagcgtg aacagctgga tgcagacgcc caccggctgg gcgatgaacg
      601 cccagggcca gttcgttccg gccggaagct ggctcgacat catcttcaat cccagcttcc
      661 cctaccggct cgtccatacg gtgcttgcgg cctacctgac gaccgcgctc gtcgtcggag
      721 cggtgggggc gtggcatttg atcaagggcc gcgacaacaa ggcggcgcgg accagcttct
      781 cgatggcgat gtggatggcg gcgctggtgg cgccggtgca gattttcgtc ggcgacctgc
      841 acgggctcaa cacgctcgag caccagcccg ccaagatcat ggcgatggaa ggccatttcg
      901 aaagctacga gaagggggcg ccgctgatcc tgatcggtca tcccgatcgg gcggcggaaa
      961 agacccgggt tctgatcgag gtgcccaaat tgtcctcgat catcctcaag cacgatcccg
     1021 acgccccgct cgccgggctg aagacgatcc cgaagtcgga ccggccgccg gcggagatcg
     1081 tcttctggtc gttccggatc atggtcgggc tcggcctcct catgttcgcg ctcgggctgt
     1141 ggagcctgct cgcccggctg cgtaagcggc tctacgactg gcgctggctt caccgtttcg
     1201 cggtggcgat ggggccggcg gggttcgtcg cggtgatcgc gggctgggtg acgaccgagg
     1261 tcgggcggca gccgtgggtg atctacggcc tgctgaggac gaaggatgcg gtctcgccca
     1321 tcgcctcgcc cggggtgacg gggtcgctga tcgccttcgt cctggtctat ttcgcggtgt
     1381 tcggggcggg gacgatgtac atcctgaagc tgatggcaaa ggcgcccgag gaaggcgagg
     1441 gggaggagcc gaggactccg atccggacgg cggggatcac gccggcggcg ggcgagagga
     1501 ggggcggatg aggggttgct ttgcctcccc gcgcgaagcg gggggagggg gaccacacga
     1561 agtgtggtgg aggggtaact gccgtggctt aggcggttcc cgcgctaacc ttgcagatcc
     1621 gcgccatcta cccctccacc atgcttcgca tggtccccct ccccatctgc gatggggagg
     1681 caaatagtgg acctcacaat cgtctgggcg ttcctcatcg cgttcgcggt tgccgcttat
     1741 gtggtgatgg acgggttcga cctcggcatc gggatcctct tccccctctt cccggtgggc
     1801 aaggagcggg acggggcgat gaacgcgatc gctccggtgt gggacggcaa cgagacctgg
     1861 ctggtgctcg gcggcggcgg cctgctcgcg gcctttccgc tcgcttatgc gatcatcctg
     1921 cccgcgctct acgcgccgct catcgtcatg ctgctcgggc tcgtcttccg cggagtggcc
     1981 ttcgagttcc gctggcgcga cgccggccat cgccggtggt gggattcggg cttcttcctc
     2041 ggctcgctgg ccgcgacctt cgcccagggc gtgactttgg gagcgctgat ccagggcatc
     2101 accgtcgagg gccgcgccta tgccggcgga tggtgggagt ggctcagccc gttcagcctc
     2161 ctgaccgggg cgagcctcgt cgcgggctac gcactgctcg gcgccggctg gctgatctgg
     2221 aagtcggaag ggccgctgca ggaccgggcg aggcgccggg cgcgtctgct ccttcccgcc
     2281 ttgctcgccg cgatcggagc ggtcagcctc tacaccccgt tcgtcgagcc caaatatttc
     2341 gagcgctggt tccaaatgcc cggactcttc gtcgccgttc cgatgccgct cctggtcgcc
     2401 gccgccgcct ttttcggctg gcgaagcctc gggcgcgggc gggactgggc gccgttcgtc
     2461 tggtcgctgg cgttgttcgg gctcaccctc gcgggcctcg ccatctcgat ctggccggac
     2521 gtgatccccg ggcgggtcag catctggcag gccgcctccc ctcattccag ccagctcttc
     2581 atgctggtcg gagccctcgt cctcatcccg ctcatcctcg cctacaccgc ctggagctat
     2641 tgggtgttcc gcggcaaggt cggggaggag ggctatcact gaggtggcga agcgcctcgc
     2701 ctggttcgtc gggctgtggg ccgcgggcgt ggcgtcggtc gcgtcggtcg cctacctgct
     2761 tcgctggtgg ctcctttgag ccggcccgtg ctggtcgccg gagcggccct cgccggtctc
     2821 ggagtcgccc tcggcgcctt cggggcgcat gcgctgaaag cctcgctccc gccccaggcg
     2881 ctcggctggt gggacatagc ggtccagtat cagatgtggc acgcgctcgg gctgatcctg
     2941 gtcggagtgc tgccgcagcc ccgcctcggc cttcccgccg cgctgctcgg cggcggcacc
     3001 ttgctcttct ccggctcgct ctacgcgatg gcgctcacgg gcgagcgctg gctcggcgcc
     3061 gtcactccgc tgggaggcgc cgcgatgatc gccggctggg ctctcctcgc ctggcggctc
     3121 cggtccgccg cttgattccg caacgacgtc acaggtccag caggtagacg gcgtagggga
     3181 cgccgaagcg gtcggggacc cggcgctcga gcctgaaccc catgcgcaga tagagcgcca
     3241 gcgccacctc catagccggg ctcgtatgga gggcgatcga ggcggcgccg tcgcggcggg
     3301 cgcgctcgat gcaggcgtcg gtcaggctcc gcccgaggcc tcggccgcgg gccgaggggg
     3361 cgacgaccag cattcggatc agcggccagc gcgattcgaa gaagtccgct ctcggctcgg
     3421 aaaagggcgg gcaataagcc accgctccgg cgactcgtcc gtccacctcg gcgatcagga
     3481 gctcggcatc cgccgccatg gcggacgtgc ggcccaccgc ctgctccagc gtccgccagt
     3541 catcgtagac gccgcgatat tgttcgaacg cttcgagggc gacggcgtcg accgccgctg
     3601 agtcggccgt ctcgaaggca cggatcagga tcgacgccgc tccgtcatcg ccgcccgctt
     3661 cacccgccat catcctctcc gattcccaga gcctcggccc ggtatgaggc cttggcccgc
     3721 ggggtggaag tgctggagcg ggtgttgcgg tagcctcatc ctccccacga gctccgctcg
     3781 tggggaggat tatctattcc agatcgaagc ggatgaagcg gccggcttcg gcgtggcgca
     3841 ccgactgcgc ttggaggcgc tccagcatga acttgggcat tttccggcgc agcgggcctt
     3901 tcttgtccat gatcagcgtc gtcgcgcccc agcggcgggc gaggcggagc cgctcgtctc
     3961 ggctcagcgt ggggtcgaac aggcgctcgg cggcctcctg gcgctccgcc aggccgtcga
     4021 tcatcggctc gggccacggc accgagacga ctcgctggcc ggtggcgacg atcggccagg
     4081 cggagacgtc gtaggccgcg accggccggg aatcgggaat gtcgcgggtg agggccagag
     4141 cggccgtata ggcattgcct tccctggcga atttctcctg ctcggccatg agctgggccc
     4201 gcgtcaagac gacgctcacg cccacgcaga gcaaagcgaa ggtgaagagg ccgagctggg
     4261 cggacctcgg cagggtccgc cagtcgtcga ccagggcgag gaacagggcg ccgagcccga
     4321 tctggagcat cagcaccgcg ggcatgacga agcgggtggc gatgagcagc ccgaaccagc
     4381 ccagggcgta gattccggcg aacaaagcga aggcggcgag c
//
LOCUS       DASWNU010000479         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_96553, whole genome shotgun sequence.
ACCESSION   DASWNU010000479 DASWNU010000000
VERSION     DASWNU010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96553"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /gene="mutS"
                     /locus_tag="VGB57_09425"
     CDS             complement(<1..1854)
                     /gene="mutS"
                     /locus_tag="VGB57_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0131613.1"
                     /translation="MATQAKTGTAPTPMMAQYLALKAEASDCLLFYRMGDFFELFFDD
                     AKAAAQCLDIALTARGEHEGEPIPMCGVPIHAAEAYLARLIRGGFRVAIAEQTENPAE
                     AKKRGAKSVVNRAIVRVVTAGTLTEEALLDSRASNWLVAVARTGERFGLAAADVSTGR
                     FELGSIAPTALDAELARLGAAEIVAAEPLANLPVTQRKGGFDSLAAERRLKERFGVAT
                     LDGFGAFDRAALCAAGGLLSYLDEVAKESLPFLRPPVARASGDHMMIDAATRESLELT
                     QAATGGRAGSLLHAVDRTVTGAGARLLAADLGAPLLDKAAIEARLDLVASFESDSGLR
                     ETLRRQLKALPDIGRALGRLVAGRGGPRDLGQLRDGLNETRRLHARLAALPEAPPLLA
                     SLLPALTGHGELTDLLDRALVPSPPIDSAQGGYIAEGYDAALDDLRAAGGEGRRVIAG
                     LEARYRQQTGIATLKIRHNGVLGFHIEVPARHADPLMAVDSGFTHRQTLAGVVRFNAP
                     DLHEQALRVGQAGAHALAAEAAHLEELTQAALARAEPIAAAADALARLDVASALAERA
                     VEGGWSRPRFAGHNCFDIEGGRHPVVEDSLRASGARFVANDCGLSEEERLWL"
     gene            2052..>3997
                     /locus_tag="VGB57_09430"
     CDS             2052..>3997
                     /locus_tag="VGB57_09430"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY0131614.1"
                     /translation="MSEGSSVKFSEQEALDFHTQGGRPGKIEIIASKPMATQRDLSLA
                     YSPGVAVPVRAIAEDPSKAYDYTAKGNLVAVISNGTAILGLGNLGALASKPVMEGKAV
                     LFKRFADVDAIDLELDTEDTDAFINAVALMGPSFGGINLEDIAAPACFIIEQTLRERM
                     NIPVFHDDQHGTAIITAAGLINACHLTGRDLRDIKVVVNGAGAAAIACTELIKAMGVR
                     HDHVVMCDRKGVIWQGRPEGMDQWKSAHAVITDARTLHEALVGADVFLGLSAAGALKP
                     EMVKEMAPRPIIFAMANPDPEISPPDAKAARPDAIIATGRSDYPNQVNNVLGFPFIFR
                     GALDVRATTINDEMKIAAAEALAELAREAVPEEVAAAYGGKAHSFGPDYIIPAPFDPR
                     LMEIVPAAVARAAMASGVAQIRIDDMDAYRSRLKSRLNPTTSVLSLAYEAAKAEPKRV
                     LFAEGDEDVVLRAAIAFRDGGYGIPVLVGRDDVHDRLRELGVDDPESFELLNSRTYGR
                     VPEMVDYLYRRMQRRGYLQREIERMVNQDRNTFAALLLALGEGDALITGVTRPYAQSL
                     RQMQRVLDPKAGMTPFGLHVLVGQSHTTFIADTTVTERPSGEQLADIAVHTAQVARRM
                     GHEPRVAFLSYSNFGDPEGTYLER"
BASE COUNT          641 a         1366 c         1427 g          563 t
ORIGIN      
        1 cagccacagc cgctcttcct ccgagaggcc gcaatcgttg gcgacgaagc gggcgccgga
       61 ggcgcgcagc gaatcctcga ccacgggatg gcggccgccc tcgatgtcga agcaattgtg
      121 accggcgaag cgggggcgcg accagccgcc ttcgaccgcg cgctcggcga gcgcggaggc
      181 gacgtcgagc cgggcgaggg cgtcggcggc ggccgcgatc ggctcggccc gggcgagcgc
      241 cgcttgcgtg agctcctcca ggtgggcggc ttcggcggcg agggcgtggg ctccggcctg
      301 gccgaccctc agcgcctgct cgtgaaggtc gggggcgttg aatcggacca cgccggccag
      361 tgtctggcga tgagtgaagc ccgaatcgac cgccatgagc gggtcggcat gtcgggccgg
      421 cacctcgata tggaagccga gcaccccgtt gtggcggatc ttgagggtcg cgatcccggt
      481 ctgctggcgg tagcgggcct ccagcccggc gatcacccgg cgtccctcgc cgcccgcggc
      541 ccgcaggtcg tccagagcgg cgtcatagcc ctccgcgata tagccgccct gggccgagtc
      601 gatcggcggc gaggggacga gcgcccggtc gagaaggtcg gtgagctcgc catggcccgt
      661 cagggcgggc aaaagcgagg cgagcagggg tggagcctcg ggtagggcgg cgaggcgggc
      721 gtggagccgg cgggtctcgt tcagcccgtc gcgaagctgg ccgaggtcgc gcgggccgcc
      781 gcgcccggcg accaggcggc cgagggcacg gccgatgtcg gggagcgcct tgagctggcg
      841 cctcagggtc tcgcgcaggc cggaatcgct ttcgaacgaa gcgacgaggt cgagccgggc
      901 ctcgatcgcc gctttgtcga gcagaggcgc tccgaggtcg gcggcgagga gccgggcgcc
      961 ggcgccggtg acggtgcggt cgacggcatg gagaaggctt ccggcccgcc cgccggttgc
     1021 cgcctgggtc agctcgaggc tctcgcgggt cgccgcgtcg atcatcatat gatcgcccga
     1081 ggcccgggcc accggcgggc gaaggaaggg gagcgactcc ttcgcgacct cgtcgagata
     1141 ggagagcagg ccgccggccg cgcacaaggc ggcgcggtcg aacgcaccga agccgtccag
     1201 ggtggcgact ccgaacctct ccttcagccg gcgctcggcc gcgaggctgt cgaatccgcc
     1261 cttccgctgg gtgacgggca aattggcgag cggctcggcg gccacgatct cggcagcgcc
     1321 gagccgggca agctcggcgt cgagggcggt gggggcgatg gagcccagct cgaaccggcc
     1381 ggtcgagacg tcggcggcgg cgaggccgaa gcgctcgccg gtacgcgcca ccgcgaccaa
     1441 ccagttggag gcccgggaat cgagcagggc ctcctcggtc agggtgccgg cggtgacgac
     1501 ccggacgatg gcgcggttga ccaccgactt ggccccccgc ttcttcgctt ccgccggatt
     1561 ctcggtctgc tcggcgatgg cgacccggaa gccgccgcgg atcagccggg cgagataggc
     1621 ttcggcggcg tggatcggca ctccgcacat cgggatcggc tcgccctcgt gctcgccgcg
     1681 cgcggtgagc gcgatgtcga ggcattgggc cgccgccttg gcgtcatcga agaacaattc
     1741 gaagaagtcg cccatccggt agaagagcag gcaatcggaa gcctcggcct tgagcgcgag
     1801 atattgcgcc atcatcgggg tcggggcggt tccggtcttc gcctgggtgg ccatcgggcg
     1861 agggatagag ggttggcggg cgggggggaa ttgctgaatt cgtcatcccg gcgaaagccg
     1921 gggccccggg gagacccacc gagacttctc gacctaggac cccggctttc gccggggcga
     1981 cggaaccgca ctgcgcttgc cgcgctccgc cctcaggtcc tacagacccc ccaaatcagg
     2041 gagagagccg catgagcgaa ggcagcagcg tcaaattctc cgagcaggag gcgctcgact
     2101 tccataccca gggcggccgg cccgggaaga tcgagatcat cgcctccaag ccgatggcga
     2161 cgcagcgcga cctcagcctc gcttattcgc cgggggtcgc cgttccggtg cgggccatcg
     2221 cggaagatcc ctccaaggcc tatgattata ccgccaaggg caatctcgtc gccgtcatct
     2281 cgaatgggac cgccatcctc ggcctcggca atctcggggc gctcgcctcg aagccggtga
     2341 tggaaggcaa ggcggtgctg ttcaagcgct tcgccgacgt cgacgccatc gatctcgagc
     2401 tcgataccga ggataccgac gccttcatca acgcggtcgc gctgatgggg ccgagcttcg
     2461 gcgggatcaa tctcgaggac atcgccgccc cggcctgctt catcatcgag cagacgttgc
     2521 gcgagcggat gaacatcccg gtcttccacg acgaccagca cggcaccgcc atcatcaccg
     2581 ccgccgggct gatcaacgcc tgccacctca ccggccgtga cttgagggac atcaaggtgg
     2641 tggtgaacgg cgccggggcg gccgcgatcg cctgcaccga gcttatcaag gcgatgggcg
     2701 ttcgccacga ccatgtcgtc atgtgcgacc gcaagggcgt catctggcag gggcggccgg
     2761 agggcatgga ccagtggaaa tcggcccatg cggtgatcac cgacgcccgg accctgcacg
     2821 aggccctggt cggcgccgac gtcttcctcg gcctctccgc ggccggcgcc ctgaagcccg
     2881 agatggtgaa ggagatggcg ccgaggccga tcatcttcgc aatggccaat cccgatcccg
     2941 agatcagccc gcctgacgcc aaggcggcac ggccggacgc catcatcgcc accggccgct
     3001 ccgattatcc caaccaggtc aacaacgtgc tcggcttccc cttcatcttc cggggcgcgc
     3061 tcgatgtaag ggcgacgacg atcaacgacg agatgaagat cgccgccgcc gaggccctgg
     3121 ctgagctcgc ccgcgaggcc gtgcccgagg aggtcgccgc ggcctatggc ggcaaggcgc
     3181 acagcttcgg ccccgattac atcatccccg cgcccttcga cccgcggctg atggagatcg
     3241 tgcccgcggc ggtggccagg gcggcgatgg cgagtggagt cgcccagatc cggatcgacg
     3301 acatggacgc ctatcgcagc cggctgaagt cgaggctcaa cccgaccacg tcggtgctca
     3361 gcctcgccta cgaggccgcg aaggcggaac cgaagagggt gctgttcgcc gagggcgacg
     3421 aggacgtggt gctgcgcgcg gccatcgcct tccgcgacgg cggctacgga atcccagtcc
     3481 tggtcggccg cgacgacgtc cacgaccggc tccgtgagct tggagtcgac gatccggaaa
     3541 gcttcgagct tctcaacagc cgcacctacg gccgggtgcc ggagatggtc gactatctct
     3601 acaggcgcat gcagcggcgc ggctatctcc agcgcgagat cgagcggatg gtgaaccagg
     3661 accgcaacac cttcgcggcg cttctgctcg cactgggcga gggcgacgcc ctgatcaccg
     3721 gagtcacccg cccctatgcg cagagcctga ggcagatgca gcgggtgctc gaccccaagg
     3781 cgggaatgac ccccttcggc cttcacgtcc tcgtcggcca gtcgcacacc accttcatcg
     3841 ccgacaccac ggtcaccgag aggccgagcg gcgagcagct cgccgacatt gccgtccata
     3901 ccgcccaggt cgcccgccgg atgggccacg agccgcgggt ggccttcctc tcctattcga
     3961 acttcgggga ccccgagggc acctatctgg agcggat
//
LOCUS       DASWNU010000480         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_435182, whole genome shotgun sequence.
ACCESSION   DASWNU010000480 DASWNU010000000
VERSION     DASWNU010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435182"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGB57_09435"
     CDS             <1..385
                     /locus_tag="VGB57_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0131615.1"
                     /translation="LGAAAGRRTGLDSKTIVAALAEREKVGSTGYGGGVAIPHGRIEG
                     LKSVFGYFARLTAPIDFQSVDNLPVDLVFLILSPPDAGADHLKALASVSRVLRDRGTV
                     AKLRGARSRDALFALLSGVESRNAA"
     gene            375..836
                     /locus_tag="VGB57_09440"
     CDS             375..836
                     /locus_tag="VGB57_09440"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0131616.1"
                     /translation="MPPEADAGAAAHYRALESLYRHAPVNGLFESRLEIVEKGFARIR
                     FEVREQLFHAAGAAHGTLYFKMMDDAAFYACNSLVTDRFLLTTAFNLLFTRPLRAGPV
                     TAEGRWASGKRRVLVGEARLIDSEGELAASGTGTFMRSHIPLSGLPGYVAG"
     gene            836..1177
                     /locus_tag="VGB57_09445"
     CDS             836..1177
                     /locus_tag="VGB57_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEY0131617.1"
                     /translation="MEPRVAASVLATALIRKAEGEGGFGAVLAKGDAAAGSILVILLE
                     KGVNPRLFERLLQPDGRYAWQESGSQRIENPNEVPGFIARLRRFDPDSWLIELDIPSA
                     ERFAAEMNTLN"
     gene            1348..1977
                     /locus_tag="VGB57_09450"
     CDS             1348..1977
                     /locus_tag="VGB57_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY0131618.1"
                     /translation="MNRLFRAASFAAAAFIFAFGAPSLAWELTGTPLTAETSGQPALG
                     APPPIVSAPPVAAVEPAPAAPAEAERPGRRALAELVADHSSTQTADVQHECLAGAVYF
                     EAKGEPLHGQLSVAEVILNRARSGRFPASACGVVKQRGQFSFVRGGRFPAIARTSLAW
                     KRAVAIAHIAVQDLADGPAPKALFFHAKRVSPRWRLTRVAAVGNHIFYR"
BASE COUNT          325 a          757 c          700 g          362 t
ORIGIN      
        1 gctcggcgcc gccgcggggc ggcgaaccgg gctcgattcc aagaccatcg tcgccgctct
       61 ggccgaacgg gagaaagtgg gctccaccgg ctacggcgga ggcgtggcca tcccgcacgg
      121 ccggatcgag ggcctgaaaa gcgtcttcgg ctatttcgcc cgtctgacgg cgccgatcga
      181 cttccagtcg gtggacaatc tgccggtcga cctcgtcttc ctgatcctgt cgccgcccga
      241 cgccggggcg gaccacctca aggcgctggc gtcggtcagc cgggtgcttc gcgatcgggg
      301 cacggtggcc aagctccgcg gcgcccgctc gcgcgacgcc ctgttcgctt tgctttccgg
      361 agtcgaaagc cgcaatgccg cctgaggcgg acgcgggcgc ggcggcccat taccgggcgc
      421 tcgaatcgct ttatcgccac gccccggtca acggactgtt cgaatcgcgg ctggagatcg
      481 tcgagaaggg gttcgcccgg atccggttcg aggtgcggga gcagctcttc cacgccgccg
      541 gggcggccca tggcacgctc tatttcaaga tgatggacga tgcggccttc tacgcctgca
      601 acagcctcgt caccgaccgc ttccttctca ccaccgcctt caacctgctc ttcacccgtc
      661 ccctgcgggc gggcccggtg accgcggagg gacgctgggc gagcgggaag cggcgggtgc
      721 tggtcggcga ggcccgactc atcgattcgg agggcgagct cgccgcgagc ggcaccggca
      781 ccttcatgcg ctcccacatc ccgctcagcg ggctacccgg ctacgtcgcc ggctgatgga
      841 gccgcgagtc gccgcttcgg ttctggcgac cgccctgatt cgcaaggcgg agggcgaagg
      901 cgggttcgga gcggtcctcg ccaagggcga cgccgccgca ggctcgatcc tggtcatcct
      961 gctggaaaaa ggcgttaatc cgaggctgtt cgagcgcctc ctgcaaccgg acggccgcta
     1021 tgcttggcaa gaaagcggaa gccaacgcat tgaaaatcca aacgaagttc ccggcttcat
     1081 cgcgcggttg aggcgcttcg acccggattc gtggttaatt gaactggata tcccgtctgc
     1141 cgaacggttc gccgctgaaa tgaacaccct gaattgactc cgctgcaccg cacaacctag
     1201 ccgggcctgg tccttcgaga gcggggggcc gcaacgaacc cggggtgatt gggagggggc
     1261 ggccacgcga gacggaaggt ggcccgggat gcattctttt atcacgcatc cggcttgggg
     1321 ctgctcgccg cactgatttg agatcgaatg aaccgtttgt ttcgcgccgc gtccttcgcg
     1381 gctgccgcct tcatattcgc cttcggcgcc ccgtctttgg cctgggaact gaccgggact
     1441 ccccttaccg ccgaaacttc cggacagccc gccctcggcg ctcctccccc gatcgtttcc
     1501 gcgccgccgg tagccgcggt cgagcccgca cccgccgccc ctgccgaagc cgaaaggccg
     1561 ggacggcgcg ccctcgcgga gctggtcgcc gatcattcga gcacccaaac cgccgacgtc
     1621 cagcacgaat gcctcgccgg cgccgtctat ttcgaggcga agggcgagcc gcttcacggc
     1681 cagctcagcg tcgccgaggt gatcctcaac cgcgcccgct ccggccgatt cccggcctcg
     1741 gcctgcggcg tcgtcaagca gcgcggccaa ttctccttcg tccgaggcgg ccgcttcccg
     1801 gccatcgccc ggacctcgct cgcctggaag cgcgcggtcg ccatcgccca tatcgcggtc
     1861 caggacctcg cggacggacc ggcgcccaag gcgctcttct tccacgccaa gcgagtgtcg
     1921 ccgcggtgga ggctcacgcg cgtcgcggcc gtcgggaatc acatcttcta ccgctaggtt
     1981 cttaaatcct ccccggcctt tgccggggag ggggaccatg cgaagcatgg tggaggggtt
     2041 ctttcggagt cagcagaacc cctccaccgc gcttcgcgcg gtccccctcc cccggaaagc
     2101 cgggggagga attaagtgga tgcgggctcg cgcgaatgta aatg
//
LOCUS       DASWNU010000481         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_459232, whole genome shotgun sequence.
ACCESSION   DASWNU010000481 DASWNU010000000
VERSION     DASWNU010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459232"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VGB57_09455"
     CDS             complement(<1..389)
                     /locus_tag="VGB57_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0131619.1"
                     /translation="MKRPTVQAPRKLVTALDVGSSKVSALIAERADDGRLKILGTGQR
                     QSQGVKRGFIADMEKTEGAIREAVEQAERIAATNIEDVFVGFSAGGLVSTVASVELQI
                     GGRRIEKADIDQLLGIGRESISSDGRM"
     gene            complement(389..1318)
                     /locus_tag="VGB57_09460"
     CDS             complement(389..1318)
                     /locus_tag="VGB57_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY0131620.1"
                     /translation="MSKAIARGSQARSKARAPARGKGQRGRAAPPAPALPDAVRRLSW
                     WIFLGMMIALAGALLWALRIPQMVGGTAGESVGEMGFALKHVEIKGATHVRKIDVYNI
                     AFDQPSSAMPLVDLEATRQRLLQFGWVRDARVSRRLPDTLVVDIVERRPAAIWQHNQR
                     LALIDMDGVVLEAVRLDAMPDLPLLIGPAANLHASELARLTQAAPNLRPAMAGASWVG
                     GRRWDLRFQTGEVLALPEGEAAALKALTKFASMDQSVQLLGKGYVRIDMRDSKRMLIR
                     LPGDPGSTIPDIVPEAPPPAPAAGGDVDTARTI"
     gene            complement(1315..2235)
                     /locus_tag="VGB57_09465"
     CDS             complement(1315..2235)
                     /locus_tag="VGB57_09465"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0131621.1"
                     /translation="MTNPLHIAVLMGGWSAEREVSLTSGSGVADALESLGHKVTRIDM
                     GRDVAERLREASPDVVFNALHGTPGEDGTVQGLMDLMGLKYTHSGVETSVIAIDKELT
                     KMVLVPRGIRMPAGTMVESESLYEADPMARPYVLKPVNEGSSVGVAIVTEGGNYGDPI
                     GRDSEGPWRHFERLLAEPFIRGRELTVAVLGDEALAITELQPKSGFYDYDAKYTEGMT
                     QHICPAEVPEEIAAAAMKMAIDGHRALGCRGCSRADFRWDDEQGLAGLYLLEINTQPG
                     MTPLSLVPEQARYRGMSYAELVQKIVEEAL"
BASE COUNT          338 a          837 c          788 g          387 t
ORIGIN      
        1 atcatccgcc cgtccgagct gatcgattcc cggccgatcc cgagcagctg gtcgatgtcc
       61 gccttctcga tccggcgccc gccgatctgg agctcgacgc tggccacggt gctgaccaga
      121 ccgccggccg agaagccgac gaagacgtcc tcgatgttgg tcgccgcgat ccgctcggcc
      181 tgctcgaccg cctcgcggat cgcgccttcg gtcttctcca tgtcggcgat gaagccgcgc
      241 ttcacgccct ggctctggcg ctggccggtg ccgaggatct tgagccgccc gtcgtcggcg
      301 cgctcggcga tcagcgccga caccttcgac gatccgacgt cgagggcggt gacgagcttg
      361 cgcggggcct ggacggtcgg gcgcttcatc agatcgtccg ggcggtgtcg acgtcgccgc
      421 ctgccgccgg agccggcgga ggggcctcgg ggacgatgtc ggggatcgtg cttccggggt
      481 cgccgggcag gcggatcagc atcctcttgg aatcccgcat gtcgatccgg acatagccct
      541 tgccgagcaa ttgcaccgac tggtccatgc tcgcgaactt ggtcagcgcc ttgagcgcgg
      601 ccgcttcgcc ttccggcaga gcgagcacct cgccggtctg gaagcgcagg tcccagcggc
      661 ggccgccgac ccagctcgcg ccggccatcg ccggcctgag gttgggcgcc gcctgggtca
      721 ggcgcgccag ctcggaggcg tgaaggttgg cggcggggcc gatcagaagc ggcagatcgg
      781 gcatggcgtc gagacggacc gcctccagca cgaccccgtc catatcgatg agggcgaggc
      841 gctgattgtg ctgccagatg gccgccggcc gccgctcgac gatgtcgacg acgagcgtgt
      901 cgggaagccg cctcgagacg cgggcgtcgc gcacccagcc gaactggagc agccgctgcc
      961 gggtcgcttc aagatcgacc agcggcatcg ccgaggaagg ctggtcgaag gcgatattgt
     1021 agacgtcgat cttgcggaca tgggtcgccc ccttgatctc cacgtgcttc agggcgaagc
     1081 ccatctcgcc gacgctctca cccgcggttc cgccgaccat ctgcggaatc ctcagcgccc
     1141 agagcagggc gccggccaag gcgatcatca tgcccaggaa tatccaccag ctgagccggc
     1201 ggaccgcatc gggaagcgcc ggggccggcg gcgcggcgcg gccgcgctgg cccttgccgc
     1261 gcgccggcgc cctggccttg ctccgcgcct gcgagccgcg cgcgatcgcc ttgctcataa
     1321 cgcttcctcc acgatcttct ggaccagctc ggcatagctc atgccgcgat agcgggcctg
     1381 ctcgggaacc aggctcaagg gcgtcatgcc cggctgggtg ttgatctcga gcagatagag
     1441 gccggccaat ccctgctcgt cgtcccagcg gaaatcggcg cgcgagcagc cgcggcagcc
     1501 gagggcccga tggccgtcta tcgccatctt catggcggcc gccgcgatct cctcgggaac
     1561 ctccgccggg cagatatgct gtgtcatccc ttcggtatat ttggcgtcgt aatcgtagaa
     1621 gcccgacttg ggctgaagct ccgtgatggc gagcgcctcg tcgcccagca ccgccaccgt
     1681 cagctcgcgg ccgcggatga agggctcggc gagcagccgc tcgaaatgcc gccacggccc
     1741 ttcgctgtcg cggccgatcg ggtcgccgta attgccgccc tcggtgacga tcgccactcc
     1801 gaccgacgag ccctcgttga ccggcttcag cacatagggc cgggccatcg ggtcggcctc
     1861 gtagaggctc tcgctctcca ccatcgtccc cgccggcatc cttatgccgc gcgggacgag
     1921 caccatcttg gtcagctcct tgtcgatggc gatgaccgag gtctcgactc cgctatgggt
     1981 atatttgagg cccatcaggt ccatcaggcc ctggaccgtc ccgtcctcgc caggagtccc
     2041 gtgaagcgcg ttgaagacga cgtccggcga ggcctcgcga agccgctccg cgacgtcgcg
     2101 gcccatgtcg atccgggtga ccttgtggcc gaggctctcg agcgcgtcgg cgactcccga
     2161 gccgctggtc agcgacactt cccgctcggc ggaccagccg cccatgagga cggcgatgtg
     2221 gagggggttg gtcacagtcc gtcaccccgg cgaaagccgg ggcctcgggg agacccgccg
     2281 agacttctcg tcctgaggtc ccggctttcg ccgggatgac gggtgctcat cacttctcca
     2341 ctcccaccct
//
LOCUS       DASWNU010000482         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_278328, whole genome shotgun sequence.
ACCESSION   DASWNU010000482 DASWNU010000000
VERSION     DASWNU010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278328"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..468
                     /locus_tag="VGB57_09470"
     CDS             289..468
                     /locus_tag="VGB57_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131622.1"
                     /translation="MAEPFARHRQPWKQEEIQKLHLLAGKGMSLKAVAKALTRTEESV
                     KERAKQDGLKISKLR"
     gene            501..791
                     /locus_tag="VGB57_09475"
     CDS             501..791
                     /locus_tag="VGB57_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEY0131623.1"
                     /translation="MADTPPDRLSLNPRSPFFDGELLRRGVGIRFKGKERSDVEEYSV
                     SEGWIRVAAGKSMDRYGQPMTIKLKGEVEPFFQDSKAAEDEAAGDAGDETEA"
     gene            complement(788..1090)
                     /locus_tag="VGB57_09480"
     CDS             complement(788..1090)
                     /locus_tag="VGB57_09480"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0131624.1"
                     /translation="MTRKVPLEPYIADVLMRDLVGHDRRPSAFILYLWLWRMSRGEGR
                     PRIGASLQTLATATGLSKSAVQAAARHLAARRLVTATRTGPTEPPAYQVHEPWRRA"
     gene            1169..1534
                     /locus_tag="VGB57_09485"
     CDS             1169..1534
                     /locus_tag="VGB57_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0131625.1"
                     /translation="MITHVKFVSIPSRDQDRALDFWTSRVGFTVLSDQPFSEAQRWIE
                     LSVGNSDTRLVLFTPDGQEDRIGSFFNGALACDDVEATYRQLSERGVEFVSPPQKQPW
                     GTFATFRDPDGNQFVLSSR"
     gene            complement(1574..>2057)
                     /locus_tag="VGB57_09490"
     CDS             complement(1574..>2057)
                     /locus_tag="VGB57_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0131626.1"
                     /translation="PLDAWKDEVRALWPATAPFLDQIDSPDRLTFARYAHRTLGEPAG
                     PGLIHIGDSWHSTSPQLGQGANMALLDAWALATALEEEDGLEPALRRAVSLRRSHVRL
                     YQALSAVFTPAYQSDSRVLPWIRDRIVPPFGRLWPATRIQAEMVAGTFGGPMERLGLR
                     "
BASE COUNT          379 a          641 c          730 g          307 t
ORIGIN      
        1 tcacgaaaag tcccgccggg ctggaccggc cgccttatta accgtcatcc cggcgaaggc
       61 cgggatcaat gaacacggaa ggtcgagttc tcgctccgcc gtgttcatgg attccggcct
      121 tcgccggaat gacgtatgag aatgacgtat gaaggtgtcg atcacaggag agacggacat
      181 ggccaagggt cagaagaaaa ccaacaagga agccaggaag cccaagaagg aggtcaagaa
      241 gaccatcgcc gccaacccgt ccagcaaggg cgtcgtggcg aaggattgat ggccgagcct
      301 ttcgcccgcc accgccagcc ctggaagcag gaggagatcc agaagctcca cctgctcgcc
      361 ggcaagggca tgtcgctgaa ggcggtcgcc aaggcattga cccggaccga ggagtcggtg
      421 aaggagcgcg ccaaacagga cgggctcaag atcagcaaat tgcgctgatc gaggcccgcg
      481 cccttcacga agagaccatc atggccgaca cgccccccga ccgcctttcg ctcaatccgc
      541 gcagcccctt cttcgacgga gagcttcttc gccggggcgt gggcatccgc ttcaagggca
      601 aggagcgcag cgacgtcgag gaatatagcg tctcggaagg ctggatccgg gtcgccgcgg
      661 gcaagagcat ggaccgttac ggccagccga tgacgatcaa gttgaagggc gaggtcgagc
      721 ccttcttcca ggattcgaag gcggcggagg atgaggcggc cggggacgcc ggcgacgaga
      781 cggaagccta agcgcgccgc cagggctcgt ggacctggta ggccggaggc tccgtaggtc
      841 cggtgcgcgt ggccgtcacc agccgccgtg cagccaggtg gcgcgctgcc gcctgcaccg
      901 ccgatttgga gaggccggtg gcggtggcaa gcgtctgcag gctggcgccg atccgcggcc
      961 ggccctcgcc tcggctcatt cgccatagcc agagatagag gatgaaggcg ctcgggcggc
     1021 gatcgtggcc gacaaggtcg cgcatcagaa cgtcggcgat gtagggttcg agcggcacct
     1081 tccgggtcat acccgctact atagtgttta acgtacgcct tgtcgcggtg ggtcactgcg
     1141 ggcgagggga gggcttcacg gaggccgcat gatcacccac gtcaagttcg tcagcatccc
     1201 gagccgcgac caggatcgag cgctcgactt ctggaccagc cgggtcggct tcacggtcct
     1261 cagcgaccag ccgttcagcg aggcgcagcg gtggatcgag ctcagcgtcg gcaacagcga
     1321 cacccggctc gtcctgttca ccccggacgg gcaggaggac cggatcggat cctttttcaa
     1381 cggcgcgctc gcctgcgacg acgtcgaggc gacttaccgg cagctgagcg agcgcggggt
     1441 cgagttcgtc tcgccgccgc agaagcagcc ttggggtacc ttcgcgacgt tcagggatcc
     1501 ggacggcaac cagttcgtgc tgagctcgcg ctgaaattcc ccctcctgct tttccctaac
     1561 ccgtcatgct cgctcaacga agcccgagcc gctccatcgg gcctccgaac gtgcccgcca
     1621 ccatttccgc ctggatccgg gtcgccggcc agaggcggcc gaagggcggg acgattcggt
     1681 cgcggatcca cggcaggacg cggctgtccg actggtaggc gggggtgaac acagcggaga
     1741 gcgcctgata gagccggacg tgggagcggc gaagcgacac ggcgcggcga agcgcgggct
     1801 cgaggccgtc ttcctcttcg agcgccgtcg ccagcgccca ggcgtcgagc agggccatgt
     1861 tggcgccctg gcccagttgc gggctcgtgg aatgccagga atcgccgatg tggatgaggc
     1921 cgggcccggc gggttcgccg agggtgcggt gggcgtagcg ggcgaaggtg aggcgatccg
     1981 ggctgtcgat ttggtcgagg aaaggggcgg tggcgggcca gagcgcccgg acctcgtcct
     2041 tccaggcatc gagcggg
//
LOCUS       DASWNU010000483         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_304240, whole genome shotgun sequence.
ACCESSION   DASWNU010000483 DASWNU010000000
VERSION     DASWNU010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304240"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGB57_09495"
     CDS             complement(<1..280)
                     /locus_tag="VGB57_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131627.1"
                     /translation="MADQKSEGGTAGTGEPEGSAASGVEQRGGGDDRANQLGGGEGTG
                     AGMTGGGSATGDASEMRSEGGGGGGNGLGGPDPGSPGGMGGAGVAGATG"
     gene            complement(1439..1828)
                     /locus_tag="VGB57_09500"
     CDS             complement(1439..1828)
                     /locus_tag="VGB57_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131628.1"
                     /translation="MAKGDKKAKKDKVKIKVPKRIAGVKVSKAVRQAGKRALKLAEQP
                     AVSETVAAALLAAAAALRDPPATKRAAGAAADSAGEAGQEAIRLGDSLRKMAIDMARR
                     TLDAWESADPRPASAPHADKSGDSDRG"
     gene            complement(1842..>2141)
                     /locus_tag="VGB57_09505"
     CDS             complement(1842..>2141)
                     /locus_tag="VGB57_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131629.1"
                     /translation="VLLTTYDIQPGEIRLRAGVPVRLRFVNTTNQGLTFSAGGFFRDS
                     QLRRRDSGLVKGGSIAVPPLSTRTVVLVPKAGRYKATGANLLHRLLGMSGRIIVE"
BASE COUNT          251 a          809 c          695 g          386 t
ORIGIN      
        1 cgccggtcgc gcccgccact ccggcgccgc ccattccgcc cggcgagccc gggtcgggtc
       61 cgccgaggcc gttgccgccg ccgccgccgc cttcgcttcg catctcgctg gcatcgccgg
      121 tcgccgaacc gccgccggtc attcccgccc cggtcccttc gccgccgccg agctggttgg
      181 cgcgatcgtc gccgccgccg cgctgctcga ccccgctcgc ggccgagcct tcgggttcgc
      241 cggtgccggc ggtgccgcct tcgctctttt ggtcagccat gtcgttcgct cctctcgttc
      301 gaggcgccaa cgagcccgcc ttggggcggt tcctgttccc gcgaccgccg cctgctctcc
      361 ttcggcatcg ccatgccgac ccgaaggccg aaaatcgttc gcacaaggca cgaacgggct
      421 gcggcggcga agccgcctcg aatgtctgcc ggatcggaag gaaggctgcg cgccggcacg
      481 cgcggacccc tccgtgcctt cgtgcctttg tgcgaaaaaa tgcagcgttc ggccgaggcc
      541 cggcgctcgt cattaaccgc ctggaatcat aatgggggcc ggtgtcgcca ccggccccca
      601 cttgtcccag ctttgactgc cgtcgctggg tggcgaggcc gtctctcgct gggcggcttt
      661 ggatcgcgtc gcgtacacgt aggcccgcgc cgctctcccc cggatcccgg agcgaggccg
      721 taaagcctcg ttcggcatcc ggtgggtctc gggagaggcc gtgaggcccc ccgccgatcc
      781 ccgtcgggtc gcccgggcgg aacttgcatc ccgccgtcgc ttcccttccg gatccggccc
      841 ggccgaagcc gcgccgtctc ctcgggctcg catcgaggcc gaagcctctc cggtcgcccg
      901 tttcgaaaaa ccctgggccg aagccttccc gttcttcgag gaacctcccg aggcgtaagc
      961 ccctggaaat ccctttcgga tcgcggcgcc gcccgaaggc cttgcagctc tcccggagcg
     1021 cctctcgaag gccgaagcct cttcgaggaa ttccgttcgg gtcgcggctt gggcctgagc
     1081 ccttccccgt ttcccgccgg ctccagccgc ggggcgattc ccgcggcctt cgccggtccg
     1141 gcgggccttc cggctcgtaa agagcctttc ggcgtcgcct gggaggtttc gtgaaatccc
     1201 gcctcgcatc ggcttgcgct tatgcgggcg cggcttcgtc cgcctccctc gccttccgct
     1261 tcggggcgcc agcgtccttg ggggagcgac ggcgccttcg gcctggggca tcgcggcctt
     1321 cggtttgacg gtccgtttcc agtccgccgc ccttccggcc acgtcatgaa gctgtcagct
     1381 ttgcccgatt cgggaaaggc gaatccgcct gtggataatg aggataacgg gggtaagttt
     1441 aacctcgatc agaatcgccg ctcttgtcgg cgtggggcgc ggacgccggc ctgggatcgg
     1501 ccgattccca ggcgtcgaga gtccgccggg ccatgtcgat cgccatcttg cgaagcgaat
     1561 cgccgagccg gatcgcctcc tgcccggcct cgccggccga gtccgccgcg gcgccggccg
     1621 cacgcttcgt cgcgggcgga tcgcgcaggg ccgcggccgc ggcgagcagg gccgccgcca
     1681 ccgtctcgct caccgccggc tgctcggcga gcttcagggc cctcttgccg gcctgccgca
     1741 ccgccttcga caccttgacg cccgcgattc gcttggggac cttgatcttg accttgtcct
     1801 tcttcgcttt cttgtcgccc ttggccatct cttggaagct cctattcgac gatgatccgg
     1861 ccgctcatcc cgagcagccg gtggaggaga ttggcgccgg tcgccttgta gcgcccggcc
     1921 ttcggcacca gcacgacggt ccgggtcgac agcggcggca ccgcgatgct cccgcctttg
     1981 accagccccg aatcgcgccg ccgaagctgc gagtcgcgga agaagccccc ggccgagaag
     2041 gtgaggccct gattggtggt gttgacgaag cggagccgga ccggaacgcc cgccctcagc
     2101 ctgatctccc ccggctggat gtcgtatgtc gtcaggagaa c
//
LOCUS       DASWNU010000484         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_356133, whole genome shotgun sequence.
ACCESSION   DASWNU010000484 DASWNU010000000
VERSION     DASWNU010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356133"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1705
                     /locus_tag="VGB57_09510"
     CDS             239..1705
                     /locus_tag="VGB57_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0131630.1"
                     /translation="MTASSFRAWRSGIWLVLLLAGLSPAEAAKSRAPDLPKPAIPERF
                     NAADRYPAATVPAASLADEAWWKIFNDPVLDGFVERARKSNSSIFEAAARLEKSQALL
                     RGAAAARNPQLGGNGGASQRNGPLINESGQSGRLIYPNAILSYEIDLLGRLSKAKRAA
                     RLDAAASADLLRHARLLVEADTVQTYFEISALDEERSILRKAADDQSASLKILEERER
                     NGLATRLAVNRARAEVATLRSDIDDLDRRRAELVSGLALLMGEAPSDIAIAGKLGTPP
                     IIPSDIPSGVIARRPDISAGLRSVEAARARVGAAKVSWLPSMSLTGAQGFASPSLGSL
                     LSSATQNLGLGFLFSIPGLDGGRHKARVRSARADLSLASAEYSTQLLTALRDVDNQLS
                     AVRILGDRAGTLRSIADDDENLATMLQARFANGLISQLDYVDGLRVILEHRRTAVQVG
                     IARYVATIGLIRALGGGWGAAQPAPDRLAKSDPVQPSR"
     gene            1758..>2407
                     /locus_tag="VGB57_09515"
     CDS             1758..>2407
                     /locus_tag="VGB57_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0131631.1"
                     /translation="MADDLETFASFARDLAEAARRETLPGCRSGLAGVENKLSEGFDP
                     VTQYDRNSEAAIRRLIETRFPDHGIAGEELPERPAAGRWSWSLDPIDGTRAYICGLPS
                     WTTLIALLDEGEPVLGLVDVPRLDELYLGHSGSARMFGAGGEQRRLAASACRSVGEAR
                     LSTTDPYLFQGSEAEGFERLRRSARLTRYGLDAYGYALVAAGTLDIVAESGLKPHD"
BASE COUNT          375 a          840 c          811 g          381 t
ORIGIN      
        1 tcgcgccggt ctctccagcc gttgcggcgg ccgttgcggc ggcagcaccg acgtctatgc
       61 cgatcgttgc ggcggctcgg gccgttgcgg cggcagcagc gtctactact aagtagtagg
      121 taaatggcgt caggctgttc acgcagaccg aagcgccatc gtcaaagtgt agccatccag
      181 cttcggctgg gtggctatac ttctcagttc cgcgtcactt cgtgaagaac agcaaggtat
      241 gacggcttcg agcttcaggg catggcgttc agggatctgg ctggtcctgc tgctggctgg
      301 cttgagcccg gccgaggcgg ccaagtcccg cgctcccgac ctgcccaagc ccgccatccc
      361 cgagcgcttc aacgccgccg accgctatcc ggccgcgacc gtcccggcag cgagcctcgc
      421 cgacgaagcc tggtggaaaa tcttcaacga tcccgtgctc gacggtttcg tcgagagggc
      481 ccgaaagagc aacagcagca tcttcgaggc cgcggcgcgc ctcgagaagt cgcaagcatt
      541 gctgcgcggc gcggccgccg cacgaaaccc gcagctcggc ggtaacggcg gcgcgtcgca
      601 gcgaaacggg ccgctgatca acgagtccgg ccagagcggc cgcctgatct acccgaacgc
      661 catcctcagt tacgagatcg acctgctcgg acggctttcg aaggcgaaaa gggcggcgcg
      721 tctcgatgcc gcggcgagcg cggacctgtt gcgccacgcc cggctgctcg tcgaagcgga
      781 cacggttcaa acctatttcg aaatctccgc gctggacgaa gagcgctcga tcctgcgcaa
      841 ggcggcggac gatcaaagcg cgtcgctgaa gatcctcgag gagcgcgagc gcaacggcct
      901 ggcgacgagg ctggccgtga accgcgcccg cgccgaggtc gcgacgctcc gctccgacat
      961 cgacgatttg gaccgccgcc gcgccgagct ggtcagcggc ctcgccttgc ttatgggcga
     1021 ggcgccgtcg gacatcgcca ttgccgggaa gctcggcact ccgccgatca tcccgtccga
     1081 tatcccgagc ggcgtcatag cccggcgccc ggacatttcg gcgggccttc gaagcgtcga
     1141 agccgcccgc gcccgggtgg gggccgccaa ggtgtcctgg ctgcccagca tgagcctgac
     1201 cggcgcccag ggcttcgctt cgccctccct ggggagcctg ctcagctcgg cgacccagaa
     1261 tctcgggctc ggcttcctct tctccatccc ggggctcgac ggcggccgtc acaaggctcg
     1321 ggtgcgctcc gcccgggccg acctttcgct cgcttcggcc gagtacagca cccagcttct
     1381 gacggcgctt cgcgatgtcg acaaccagct cagcgcggtg cggatactgg gcgaccgggc
     1441 cgggaccctt cgctcgatcg ccgacgacga cgagaatttg gcgacgatgc tgcaggcgcg
     1501 cttcgctaac gggctgatca gccagctcga ttatgtcgac ggccttcggg tcatcctcga
     1561 gcaccgccgg accgcggttc aggtcggcat cgcgcgctac gtcgcgacca tcggcctgat
     1621 ccgcgccctc ggaggaggat ggggcgcggc gcagcccgcc cccgatcgtc tggcgaaaag
     1681 cgatcccgtc cagccgagcc gctgattcgt cggcagcccg cgttgacgcc gccgccgcgc
     1741 cttgtacggc ggatgtcatg gccgacgatc tggagacctt cgcaagcttc gctcgggacc
     1801 tcgcggaagc ggcaaggcgg gagacgcttc ccggctgccg atcgggtctc gcgggcgtcg
     1861 agaacaagct gagcgagggc ttcgatccgg tgacgcaata tgaccggaat tcggaagcgg
     1921 cgatccgccg gctcatcgag acccggtttc cggaccacgg cattgccggc gaggagttgc
     1981 cggagcggcc ggcggccggg cgttggtcct ggtcgctcga tccgatcgac ggcacgcgcg
     2041 cgtatatctg cggcctgccg agctggacca ccctgatcgc gctgctcgac gagggcgagc
     2101 cggtcctcgg actggtcgac gttccaaggc tcgacgagct ctacctggga cattcgggga
     2161 gcgcccggat gttcggggcc ggcggagagc aacggcgcct ggccgccagc gcgtgccgga
     2221 gcgtcggtga ggcgcggctg tcgacgaccg atccctacct cttccagggc tccgaagccg
     2281 aaggtttcga gcggcttcgg cgctcggcac gcctcacccg ctacgggctc gacgcctacg
     2341 gctatgcgct ggtcgccgcc ggcaccctcg acatcgtcgc cgaatcgggc ctgaagcccc
     2401 acgatta
//
LOCUS       DASWNU010000485         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_512097, whole genome shotgun sequence.
ACCESSION   DASWNU010000485 DASWNU010000000
VERSION     DASWNU010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512097"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1449
                     /locus_tag="VGB57_09520"
     CDS             166..1449
                     /locus_tag="VGB57_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEY0131632.1"
                     /translation="MNDVNRILMGIAGILVILGIAFLLSSNKRAIRLRVVGAAFALQA
                     GIAVLVLYTPWGRRAIGGMANGVSNLLGYANKGTEFLFGPAANNPLANTFAIAALPVI
                     VFFASLVAILYYLGIMQRIVRWVGGAIGWVTGISRVESLASAANIFVGQSESPLVVRP
                     YLAALTPAQLFTVMVVGMAGVAGTILAAYAALLGATALPYLLAAAFMSAPGGIVMAKI
                     MMPDEPRKDELPLEDGTERPASDDKVDVAETFEEGEQPANIIMAAAQGAQTGVKLAVA
                     VGAMVLAFVALVALANGLLGGVGGWFGYPELSFQRIVGTIFQPIMFLIGVPWNEAGTA
                     GGLFGTKLVLNEFVAFIDLGALPAGTLSPRSVAIVTFALCGFANFSSIAIQMAVTGGL
                     APNQRPMIARLGIKALIAGSLANLMSAALAGLLLP"
     gene            1560..2510
                     /locus_tag="VGB57_09525"
     CDS             1560..2510
                     /locus_tag="VGB57_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY0131633.1"
                     /translation="MATLLEPRPLASVSLTESDSNPEAFAQEVGRSFEEYGFAIIADH
                     GIPEDLIRRAEEKAKAFFALPDEVKRRYHVPGGGGARGYTPFGIETAKGHKAHDLKEF
                     WHVGRELPEGHPFRGPMPDNVWPEEVASFHDTLLELYDTLDRTGVKILRAVARYLGIE
                     EDYLVDSVRDGNSVLRLLHYPPIEGEPGSHIRAGAHEDINTITLLLGAEEAGLELLTR
                     EGQWIPVSPNPGELVVNIGDMLQRLTNGRLRSTSHRVVNPPRERFGHSRYSMPFFLHF
                     RSDFVIEALPGTVPAGEQPKWPTCTADEYLQERLREIKLA"
     gene            complement(2678..3127)
                     /locus_tag="VGB57_09530"
     CDS             complement(2678..3127)
                     /locus_tag="VGB57_09530"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0131634.1"
                     /translation="MTDLPDPLVERARAAALGAYAPYSRFSVGCAIESVDGEIAVGAN
                     MENACYRLGVCAEIAALTAAQQAFGLGKIARIAVAGGHVESGALAGEGVCTPCGGCRQ
                     AIYEAACLSGSDIEILCANGGGGKVDVTRIYELLPKGFGPESLKDAG"
     gene            3211..>3514
                     /locus_tag="VGB57_09535"
     CDS             3211..>3514
                     /locus_tag="VGB57_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEY0131635.1"
                     /translation="MRLATAAVLLALGGCATTEPRGAGPVEVKLIAFNDFHGNLQPPK
                     ASIDHPAPGPEAVRVPAGGAAYLASAIEALRAGNPNSLVVSAGDMIGASPLVSALFL"
BASE COUNT          558 a         1236 c         1140 g          580 t
ORIGIN      
        1 gaaaaagccg ccgaaaccag gatggtctgg taaccctcgc cgtcattgcg agcggagcga
       61 agcaatccag tgcggcctcg gacgaaggct ggattgcttc gtcgcttcgc tcctcgcaat
      121 gacggaggcg gggcgttact gctgagcgga acacgggggg aagcaatgaa cgacgtcaac
      181 cggatcctga tgggaatcgc cgggatcctg gtcatcctcg ggatcgcctt cctgctatcg
      241 tcgaacaagc gggcgatccg gctccgggtc gtcggcgccg ccttcgcgct tcaggcggga
      301 atcgccgtcc tcgtcctcta tacgccctgg gggcgccggg cgatcggggg catggcgaac
      361 ggagtctcaa acctgctcgg ctatgccaac aagggcaccg aattcctgtt cgggccggcc
      421 gcgaacaatc ccctagccaa caccttcgcc atcgccgctt tgccggtgat cgtcttcttc
      481 gcctcgctgg tcgccatcct ctattatctc ggcatcatgc agcggatcgt ccgctgggtc
      541 ggcggcgcga tcggctgggt gaccgggatc agccgggtcg aatcgctggc ctcggctgcc
      601 aacatcttcg tcggccagag cgaatccccg ctcgtcgtcc gcccctatct cgccgcgctc
      661 acgccggcgc agctcttcac cgtcatggtc gtcgggatgg cgggcgtcgc cggcacgatc
      721 ctcgcggcct atgccgccct gctgggggcg accgccctcc cctacctcct cgcggccgcc
      781 ttcatgtcgg cgcccggcgg catcgtgatg gccaagatca tgatgccgga cgagccgcgc
      841 aaggacgagc ttccgctcga ggacggcacg gagcggccgg cgagcgacga caaggtcgac
      901 gtcgccgaga cgttcgagga aggcgagcag cccgccaaca tcatcatggc cgccgcgcag
      961 ggcgcccaga ccggggtcaa gctggcggtc gcggtcggag cgatggtcct cgccttcgtc
     1021 gccctggtgg cgctcgccaa cggcctgctg ggaggggtcg gcggctggtt cggctatccc
     1081 gagctcagct tccagcggat cgtaggcacg atcttccagc cgatcatgtt cctgatcgga
     1141 gtgccctgga acgaggcggg gacggccggc ggcctgttcg gcaccaagct ggtgctgaac
     1201 gagttcgtcg ccttcatcga tctcggcgcc cttccggccg ggactctgag cccgcgaagc
     1261 gtcgctatcg tcaccttcgc gctttgcggc ttcgccaatt tctcgtcgat cgcgatccag
     1321 atggcggtga ccggcggcct cgcccccaac cagcggccga tgatcgccag gctcgggatc
     1381 aaggcgctga tcgcgggctc gctggccaat ctgatgagcg cggcgctggc ggggctgctg
     1441 ctgccctgac accgtcattg cgagcgaagc gaagcaatcc agtgcggcgc ctcctcccgc
     1501 tggattgcct cgtcgcttcg ctcctcgcaa tgacgggtcg ttgcgggtaa aagcccccca
     1561 tggcaacgct cctagaaccc cgcccgctcg cctccgtctc gctgaccgag tccgactcga
     1621 accctgaagc ttttgcccag gaagtgggcc gaagcttcga ggaatatggc ttcgcgatca
     1681 ttgccgacca cggcatcccc gaggacctaa tccgccgggc ggaggagaag gcgaaggcct
     1741 tcttcgccct ccccgacgaa gtgaagcgcc gctaccacgt ccccggcggc ggcggcgcgc
     1801 gcggctacac gcccttcggg atcgagacgg ccaagggcca caaggcgcac gacctcaagg
     1861 aattctggca tgtcgggcgc gagctgcccg aaggccaccc cttccgcggg ccgatgcccg
     1921 acaacgtctg gccggaggaa gtggcgagct tccacgacac cttgctcgag ctctacgaca
     1981 cgctcgaccg caccggcgtg aagatcctca gggccgtcgc ccgctatctc ggcatcgagg
     2041 aggattatct cgtcgactcg gtccgcgacg gcaattcggt gcttcgcctc ctccattacc
     2101 ccccgatcga gggcgagccg ggaagccata tccgagccgg cgcccacgag gacatcaaca
     2161 ccatcaccct tctgctcggc gccgaggaag cggggctgga gctcctcacc cgggaagggc
     2221 aatggattcc cgtgtcgcct aatccgggcg agctcgtcgt caacatcggg gacatgctcc
     2281 agcggctgac caacgggcgg ctgcgctcaa cctcgcaccg ggtcgtcaac ccgccgcgcg
     2341 agcgattcgg ccattcgcgt tactcgatgc ccttcttcct tcacttccgc tcggacttcg
     2401 tcatcgaggc cctgcccggc acggtcccgg ccggcgagca gccgaaatgg ccgacctgca
     2461 cggccgacga atatctccag gagcggctga gggagatcaa gctggcctga tttgcctccc
     2521 catcgaagat ggggggggga ccaggcggag cctggtggag gggtagatgg cgcaggcttc
     2581 cgccgtcgtg cgcatgaacc tctgggtcca cggcagttac ccctccacca cgcttcgcgt
     2641 ggtccccctc cccaccgctt cgcggcaggg aggcagatca gcccgcgtcc ttcaacgact
     2701 ccggcccgaa acccttcggt agcagctcgt agatcctggt cacgtccacc ttgccgccac
     2761 ccccattggc gcagagtatc tcgatgtccg accccgacag gcaggccgcc tcgtaaatcg
     2821 cctggcggca gccgccgcac ggcgtgcaga cgccctcccc ggccagagca ccgctttcga
     2881 catggccgcc cgccaccgcg atccgggcga tcttgccgag cccgaaggcc tgctgggccg
     2941 cggtcagagc ggcgatctcg gcgcagaccc cgagccggta gcaggcattc tccatattgg
     3001 cgccgaccgc gatctcgcca tcgaccgatt cgatcgcgca gccgacgctg aagcggctgt
     3061 agggggcata ggcgccaagc gctgcggccc gggcgcgctc gacgaggggg tcgggaaggt
     3121 ctgtcatgcg catcatccat aaagccattg gcgattcgcg ccagaccatg gcacgaaccc
     3181 cgcgtcttgg cggagaatga gatgacgagg atgaggttgg cgacggcggc ggtcctgctg
     3241 gcgctggggg gttgcgcgac gacggagccg cgcggggcgg gcccggtcga ggtgaagctg
     3301 atcgccttca acgacttcca cggcaatctt cagccgccca aggccagcat cgaccatccg
     3361 gcgccggggc ctgaagcggt ccgggtgccg gccggcggcg ccgcctatct cgcctcggcc
     3421 atcgaggcgc ttcgcgccgg caaccccaac agcctcgtcg tttcggcggg cgacatgatc
     3481 ggcgcttcgc cgctcgtgtc ggccctgttc ctgg
//
LOCUS       DASWNU010000486         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_381890, whole genome shotgun sequence.
ACCESSION   DASWNU010000486 DASWNU010000000
VERSION     DASWNU010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381890"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VGB57_09540"
     CDS             <1..713
                     /locus_tag="VGB57_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HEY0131636.1"
                     /translation="KLGFDDSAILPAGEESSRHERMSAERERIGPVNLIAERELAELD
                     ESRTASQAEREELGLAINRLRGSIGSLNREGRARLLTAFETVDCHFRSLFTTLFEGGQ
                     AHLELVESDDPLEAGLEIMAQPPGKRLSTLTLLSGGEQALTAIALIFALFLTNPAPIC
                     VLDEVDAPLDDANIERFCDLLDRMVNETETRYLIVTHNAVTMSRMHRLFGVTMIEKGV
                     SRLVSVDLGGAERLLAAE"
     gene            752..1813
                     /locus_tag="VGB57_09545"
     CDS             752..1813
                     /locus_tag="VGB57_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131637.1"
                     /translation="MADVKAGLLAAGASGTIYSLVMPESEQAFAAVVGEEDFLDRYRE
                     PWTQKQDAMHETYFEQWLRWSAPVVALRGEDFPFRYPTAGASEAIFKLIAEFASERRA
                     AGGEPAVHMFEGEYEGFAAYADSLKVRIVRHDRGAWSEVPARLDSNAQFWISQPSAID
                     GMVWPHFEAFATAIAEAGRGAQLVPDLTYVGSVARDYRIALGSPAIPAVVISHSKPFG
                     GYYHRVGGVLARREHPSLFGNKWFKNLLSLAWGTEMMRRHGIFDLPRKYRPVQEEAAR
                     RVTALLDVDGLAAADVLLLATAPAKAGLPPLLASVLRGSPGEQVVRLCLTPTMTCLID
                     PAMAPATAPGLEAGWSGRG"
     gene            1810..2709
                     /locus_tag="VGB57_09550"
     CDS             1810..2709
                     /locus_tag="VGB57_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131638.1"
                     /translation="MKPESIELLAEPADIHDFVMSLWSDGPIRRSHASGGSVKRLVDR
                     FARLPRFFFRASDQGLEWTHFSTWWGGILLCDYAKPAIRDLRYLHEIHHAATLPYIPN
                     GNVPTFEAKAFRNEREASTATEMAIYLEFPELRPLTFDHPIFMDRFLFPDGDFGRPDA
                     RLIERWRSERDIVFQELMYERTRAILADESEVDPDDPQIVWLRRYGEQGANWVRVWSR
                     RFQCVEDSMIRLREDGARGGRKDAARRHLDWLLSPEIAEGTDVPFRTEAAGFRASFDA
                     LIHVYDEAMVQARQIPVKGKGDI"
BASE COUNT          465 a          967 c          954 g          450 t
ORIGIN      
        1 agaagctcgg tttcgacgat agcgccatcc tccccgccgg cgaggaatcc agccgccatg
       61 agcggatgtc ggcggagcgc gagcggatcg gcccggtcaa cctcatcgcc gagcgcgagc
      121 ttgccgagct cgacgagagc cgcaccgcct cgcaggccga gcgcgaggag ctcggccttg
      181 cgatcaaccg gctgcgcggc tcgatcggaa gcctcaaccg cgagggccgg gcccgactgc
      241 tcaccgcctt cgaaaccgtg gactgccatt tccggagcct gttcaccacc ctcttcgaag
      301 gcggccaggc gcatctcgag ctggtcgaat cggacgatcc gctcgaggcc gggctcgaga
      361 tcatggccca gccgccgggc aagcgcctct ccaccttgac cctcctgtcg ggcggcgagc
      421 aggcgctgac cgcgatcgcc ctcatcttcg ccctcttcct caccaacccg gcgccgatct
      481 gcgtcctcga cgaggtcgac gcgcccttgg acgacgccaa tatcgagcgt ttctgcgacc
      541 tgctcgaccg gatggtgaac gagaccgaga cccgctatct gatcgtcacc cacaatgcgg
      601 tgacgatgag ccggatgcac cgcctgttcg gggtgaccat gatcgagaag ggggtgagcc
      661 gcctggtcag cgtcgatctc ggcggcgccg agcgcctgct cgcggcggag tgatggccgg
      721 gcgccggccg gcggaccttc ctggctacgc catggccgac gtcaaggcgg ggctgctggc
      781 ggcgggggct tcgggcacga tctactcgct ggtcatgccg gaatcggagc aggcgttcgc
      841 cgccgttgtc ggcgaggagg atttcctcga ccgctaccgc gagccctgga cccagaagca
      901 ggatgccatg cacgaaacct atttcgagca atggctgcgc tggtccgccc ccgtcgtcgc
      961 cctgcgaggc gaggacttcc ccttccgcta cccgaccgcc ggggcgagcg aggcgatctt
     1021 caagctcatc gccgaattcg cctccgagcg ccgggcggcc ggaggcgagc cggccgtcca
     1081 catgttcgaa ggcgaatatg aaggcttcgc cgcttatgcg gactcgctca aggtgcggat
     1141 cgtgcggcac gaccgcggcg cctggagcga ggtcccggcg aggctggact cgaacgccca
     1201 gttctggatc agccagccct cggcgataga cgggatggtt tggccccatt tcgaggcctt
     1261 tgcgacggca atcgcggagg cggggcgcgg ggcgcagctg gtccccgacc tcacttacgt
     1321 cggaagcgtc gcccgcgact atcggatcgc gctaggttca cccgccattc cggcggtggt
     1381 gatcagccac agcaagccgt tcggtggcta ttatcaccgt gtcggcggag tgctggcgcg
     1441 gcgtgagcat ccctccctgt tcggcaacaa atggttcaag aacctgctgt cgctggcctg
     1501 gggtaccgag atgatgcgcc gccacggaat cttcgacctg ccccgcaaat accgcccggt
     1561 ccaggaggag gcggcgcggc gggtgaccgc cctgctggac gtcgacggcc tcgccgccgc
     1621 cgacgtgctg ctgctcgcca ccgcccccgc caaggccggg ctgccgcccc tgctcgccag
     1681 cgtgctgagg ggcagcccgg gagagcaggt ggtacggctg tgcctgactc cgaccatgac
     1741 ctgcctcatc gatcccgcca tggccccggc gacggcgccc ggcctcgagg ccgggtggag
     1801 cggccgcgga tgaagccgga gagcatcgag ctgctggccg agcccgccga catccacgat
     1861 ttcgtgatga gcctgtggag cgacggcccc attcgccgct cccacgcctc cggaggctcg
     1921 gtgaagcgcc tggtcgaccg gttcgcgcgc ctgccccgct tcttcttccg ggcctcggac
     1981 caagggctcg aatggaccca tttctcgacc tggtggggcg gaatcctgct gtgcgactac
     2041 gccaaaccgg cgattcgcga ccttcgctac ctccacgaaa tccaccatgc cgcgaccctg
     2101 ccctacatcc cgaacggcaa cgtcccgacc ttcgaggcca aggcgttccg caacgaacgc
     2161 gaggcgagca ccgccaccga gatggcgatc tatctggaat tccccgaatt gcggccgctg
     2221 accttcgacc atccgatctt catggatcgc ttcctcttcc cggacggcga tttcggccgg
     2281 cccgatgcgc gcctgatcga gcgctggcga agcgagcggg acatcgtctt ccaggagctg
     2341 atgtacgagc gcacccgggc catcctcgcc gacgagtcgg aggtggaccc ggacgatccg
     2401 cagatcgtct ggcttcggcg ctatggggag cagggcgcca attgggtcag ggtctggtcg
     2461 cggcgcttcc agtgcgtcga ggattcgatg atccggcttc gcgaggacgg cgcgaggggc
     2521 ggccgcaagg acgccgcccg acgccatctc gactggctgc tctcgcccga aatcgccgaa
     2581 gggactgacg tacccttccg gaccgaggcg gccgggttca gggcgagctt cgacgctctg
     2641 atccacgtct acgacgaagc catggtccag gcgcgccaga ttccggtaaa gggcaaaggg
     2701 gatatatgag cacgaagcgg cggaatgttt tgcctcccca tcgaaaggtg gggaggggga
     2761 ccatgcgaag catggtggag gggtaaatgg cgagggaagc ggagggtcaa gcgggaaccg
     2821 ccgacatcgg agcagt
//
LOCUS       DASWNU010000487         6821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_96829, whole genome shotgun sequence.
ACCESSION   DASWNU010000487 DASWNU010000000
VERSION     DASWNU010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96829"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1852)
                     /locus_tag="VGB57_09555"
     CDS             complement(41..1852)
                     /locus_tag="VGB57_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HEY0131639.1"
                     /translation="MRSSVSKARAAAVDPTSAWAEAAVRGDFVVGESVRYSAERHLRD
                     LRDAESRGYFWRPELAQRALNFFPSLFTITDGPAEGKPFTLIPYQTFCVGSLMGWVNA
                     EGRWRFRSGYIETGKGQAKSPMMAGLGLYAMGWCGFNRSQVYSIAANKQTSMVLFKDA
                     TAMCRAPVPGYDEDETLEGLGHVVIRGEGDNAWKIEHPGSQSFFLPLADGKQQSGPRP
                     RMVLADEIHEFSTEAQLNIWARAIDKIAGSAMMVLGSNTPAKSQHVGSSISNVAQAVA
                     RGDVKNDTAFAFVARIDKADRDTVFENEACWRKSLPALGITYPITNIREAVETARTRP
                     STASSVKRLYFGIDDGAVDFWINEADWAAVQSEIGDQKVAELRGCSCWLSLDLSKKND
                     LTALTATWIDDDGHLWSKTWYWTAREGLAERAAEDLAPYEDWIEEGFLAAVGGATIDY
                     TFVAEQVKQLCSDHDVQELVFDPAKMGDFETACDDIGFPAWRFKSPDEPAGTGLKMVS
                     HAQGTKVVFQDRQHCMPRSIEKLEDRILNRTITIDSSPVTYSCAANAVLDEDGMKNRA
                     FNKKRSRGRIDGVVTIAMGAGAADNPIATKKFKYTGI"
     gene            complement(1827..2339)
                     /locus_tag="VGB57_09560"
     CDS             complement(1827..2339)
                     /locus_tag="VGB57_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEY0131640.1"
                     /translation="MARAPAAKLSGAAAAAPRKGKGTKAPPRRKPVEDPRQEGEPDWK
                     QLLPDVGERAAARAHWQAIAGEMRERETFSVANSHALLRLVLAYLVYDRCSVIVAVDG
                     LVTAPNPENPKSIARLSIHYKAMAEAEKTAERLEAQLGLSPGRRSRVGKVTKKRERRA
                     DADAFLGQQG"
     gene            complement(2780..3139)
                     /locus_tag="VGB57_09565"
     CDS             complement(2780..3139)
                     /locus_tag="VGB57_09565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY0131641.1"
                     /translation="MADGAGRARRPWARAEGTPDKRKRGRAGQRDRQRRLARTGGLCE
                     HCLDEGRTTLASVVNHKIPLARGGEDVDENTENLCGPCDVAETARQFGLAQPIQGRGI
                     GEDGRPTSPDHPWNRRL"
     gene            complement(3314..3598)
                     /locus_tag="VGB57_09570"
     CDS             complement(3314..3598)
                     /locus_tag="VGB57_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131642.1"
                     /translation="MKLPSMRCWRDFLFGLLNNMNETAWHHEDRAFCPVLWSAPLGLA
                     VVMPRAAMLGPGEFYDVAPKLPALPGVERKASSWGRLNGELVAVDYGWRG"
     gene            complement(3655..4173)
                     /locus_tag="VGB57_09575"
     CDS             complement(3655..4173)
                     /locus_tag="VGB57_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEY0131643.1"
                     /translation="MTVRVPRTVGQATAMLERYAAITGELARAEERRSRLIVRANAAA
                     DVITAPLVTEAGAILLKLEGWWKTAGPDLAGKRKSIELGGCMIGSRASKPKLVHGFES
                     DDKAVEALRGTRHGKQTTRLKYSLDRPATLKLLQLKGGTAEAIAELGFSIDQGERFFV
                     QRAEQAGTIGAA"
     gene            complement(4170..4391)
                     /locus_tag="VGB57_09580"
     CDS             complement(4170..4391)
                     /locus_tag="VGB57_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131644.1"
                     /translation="MDKPLDIVMGEPLDIDALRRSASRTDSPNAVVCRKWLARVALEI
                     AEGRAAKAQLAMHAGMTHVIDSLQSGAQR"
     gene            complement(4391..4702)
                     /locus_tag="VGB57_09585"
     CDS             complement(4391..4702)
                     /locus_tag="VGB57_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131645.1"
                     /translation="MSTHSAPPGGRRRPAAPAAAPAEIRSGQGRRPTIYEGLRALGSR
                     FDQLQWMASQDSRSQRDHDNIVDEAEAIAGDLRALVRGRSGPEAVNPPLWDNGRGRAA
                     W"
     gene            complement(4870..5547)
                     /locus_tag="VGB57_09590"
     CDS             complement(4870..5547)
                     /locus_tag="VGB57_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131646.1"
                     /translation="MANRPALRPAPTGKRRKNDRRPELQRAIEQHQEANRILWARRHP
                     EAAAAERSLRKGRAELLDLWSHKNAGTPETHDHASRCNQGALARLYENGTISGEQLAA
                     GVEIAQVAEKIGADVSVRTASLETRVDVTRMGDGTFYERLGQVRREVAYTRWRGQVEG
                     PIGAVLDMIVGEPVGFTVVAKRYRIHNRKAKRLLLEALDLWSRILGQVAKDIDAATLA
                     AAQAAIL"
     gene            complement(5655..6068)
                     /locus_tag="VGB57_09595"
     CDS             complement(5655..6068)
                     /locus_tag="VGB57_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131647.1"
                     /translation="MRYSRAMGEALPIDPTGNRRLKPTMRSRKLQALDFIKRYFAEWG
                     HSPSLDEIGAALEVSKQRASELVQQLCDEDQIQRVVGKRRGIVLIDRGEQLSEADVLL
                     RIKQLGWSVNASEGTVGRTLTQTGLPDVPELDYKG"
     gene            complement(6138..6548)
                     /locus_tag="VGB57_09600"
     CDS             complement(6138..6548)
                     /locus_tag="VGB57_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131648.1"
                     /translation="MAVTSIYRDTNVTRLSLGKSLDGQFKMIIHNDAWSAEENKLYEL
                     YLLVDDKLFSGRGLGLSGGTLLLNLKPEVMQAIIKGARLAPALKIDDDNERVLEAMKL
                     TGSAGAMARVATCVAAAAEANRRAERFPSDPFKR"
BASE COUNT          995 a         2350 c         2230 g         1246 t
ORIGIN      
        1 gcccgcagcg cggcgcgagc acggttcatc atccccatgc tcagatccct gtatatttga
       61 atttcttggt ggcgatcggg ttgtcggccg ccccggcgcc catcgcgatc gtcacgactc
      121 cgtcgatccg gccgcgcgag cgtttcttgt tgaaggcccg gttcttcatg ccgtcttcgt
      181 cgaggaccgc attggccgcg cagctgtagg tcacgggcga gctgtcgatc gtgatcgtcc
      241 ggttgaggat cctatcctcg agcttctcga tcgagcgcgg catgcaatgc tgccggtcct
      301 ggaaaacgac cttcgtgccc tgagcatggg agaccatctt caggccggtg ccggccggct
      361 cgtccggtga cttaaaccgc caggccggaa agccgatgtc gtcgcaggcg gtctcgaagt
      421 cgcccatctt ggccgggtcg aagaccagct cctggacgtc gtggtcgctg cagagctgct
      481 tcacctgctc ggcgacgaag gtatagtcga tcgtggcgcc gcccaccgcg gcgaggaagc
      541 cttcctcgat ccaatcctca tagggcgcca gatcctcggc ggcgcgctct gccaggcctt
      601 cccgcgctgt ccagtaccaa gtcttcgacc agaggtgccc gtcgtcgtcg atccaggtcg
      661 ccgtgagcgc cgtgaggtcg ttcttcttcg acaggtcgag gctcagccag cagctgcagc
      721 cccggagctc cgcgaccttc tggtcgccga tctcgctttg gaccgcggcc cagtcggcct
      781 cgttgatcca gaagtcgacg gcgccgtcgt cgattccgaa gtagagccgt ttgaccgagg
      841 aagccgttga cggccgggtc ctggcggtct cgaccgcctc gcggatgttt gtgatcggat
      901 aggtgatccc gagcgcggga agggacttcc tccagcacgc ctcgttttca aaaaccgtat
      961 cgcgatcggc tttgtcgatc cgggctacga aggcaaaggc cgtgtcgttc ttgacgtcgc
     1021 ccctcgccac ggcctgggcg acgttggaaa tggaggatcc gacgtgctgt gacttcgccg
     1081 gcgtattgct cccgagcacc atcatggcgc tgccggcgat cttgtcgatc gctcgcgccc
     1141 atatgttgag ctgggcctcg gtcgagaatt cgtggatctc gtccgccagc accatgcgcg
     1201 gccgaggccc ggattgctgc ttcccgtcag cgagcggcag gaagaaggat tgcgagcccg
     1261 gatgctcgat cttccaggcg ttatcgcctt cgccgcggat gacgacgtgc ccgagcccct
     1321 ctagggtctc gtcctcgtca taccccggca cgggcgcccg gcacatcgcc gtcgcgtcct
     1381 tgaagaggac catcgacgtc tgtttgttcg ccgcgatcga atagacctgc gagcggttaa
     1441 acccgcacca gcccatcgcg tagaggccga ggcctgccat catcggcgac ttggcctggc
     1501 ccttgcccgt ctcgatgtag ccggaacgga agcgccagcg cccctccgcg ttgacccagc
     1561 ccattagcga gccgacgcag aatgtctgat acgggatcag ggtgaacggc ttgccttcgg
     1621 cgggaccgtc agtgatcgtg aatagcgacg ggaagaagtt gagcgcgcgc tgggcgagct
     1681 ccggacgcca gaaataccct cgcgactccg cgtctcgaag gtcccgaagg tgccgctcgg
     1741 cggaatagcg gaccgattcc ccgacgacga agtcaccgcg gaccgctgcc tcggcccagg
     1801 ccgaggtcgg gtcgaccgcg gccgctctag ccttgctgac cgaggaacgc atcggcatcg
     1861 gccctgcgct cccgcttctt cgtgaccttc ccgacccggc tgcgccggcc tggcgagagg
     1921 cccagctgcg cctcgaggcg ctctgcagtc ttctcggcct cggccatcgc tttgtagtgg
     1981 atcgacagtc gagcgatcga cttcgggttc tccgggttgg gcgctgtcac cagcccgtca
     2041 accgcgacga tgactgagca gcgatcatag accaggtacg cgaggaccag gcgcagcagc
     2101 gcgtggctat tcgcaactga gaacgtctcc cgctcgcgca tctccccggc gatcgcctgc
     2161 caatgcgccc tcgccgccgc ccgttctccg acgtcgggga gcagctgctt ccaatcgggc
     2221 tcgccctcct ggcgtggatc ctcgaccggc ttccgcctgg gcggcgcctt cgtccccttc
     2281 cccttgcggg gcgccgcggc cgcggcgccg ctcagctttg ccgcgggcgc tcgagccaca
     2341 gctcaggccg cgcgacggtg cttgtggcgc acccgcctcg aggtatgcag ccggttcgtc
     2401 cggaggcgcc gcctggtcgg cgggttcttc gaccggcgat cgaccggcgc cgcctgggac
     2461 tcgaggcgcc gaggccggcg ctgaccgcgc cgcgtttcgt cgtcgacctc gggaatcggg
     2521 gcgaccggcg ccgcggcggc cacggcggac aggcaaacgc tggccgccaa ggcccaacct
     2581 atgttaacac tctgcatcaa ccaccccccc caacttttca gtttcaaatc gctcgcggtg
     2641 cgtatggccc cactgcgcgg tgtccggttt ggccgacccc agggattgac cctccccccc
     2701 ctgcccctca ggggcacggc ggggttagag aggacggtct caggggtggc cggctctgcc
     2761 ccgccggggc acggcggggt tagagacgtc ggttccaggg gtggtcgggg ctggtcggcc
     2821 ggccatcctc gccgatgccg cggccctgga tgggctgggc caggccgaac tggcgcgccg
     2881 tctccgcgac gtcgcatgga ccgcataggt tctcggtgtt ctcgtcgacg tcctcgccgc
     2941 cccgggccag tggtatcttg tggttgacga cgctggccag cgtcgtgcgg ccctcatcga
     3001 ggcaatgctc gcagaggccg ccggtccggg ccagccggcg ctggcgatcg cgctggccgg
     3061 ctcggccgcg cttgcgcttg tccggcgtgc cctcggcccg agcccatggt ctccgggccc
     3121 ggccggcgcc gtcagccacg aggacggcgc accgtggagt agagcgagaa gaagccgaat
     3181 cgccgcggtt cgaggggccg attacagggg cagccgggat ccgcgcagcg gccgcccggc
     3241 acgttcgatc cgaacggact ggtcccatcg tcgccggcgt ccatgactag gctccgcgct
     3301 gcatcggcat ggatcagcct cgccacccgt agtcgacggc gacgagctcg ccgttcagcc
     3361 tgccccagct gctcgccttg cgctcgactc cgggcagcgc cgggagctta ggcgcgacgt
     3421 cgtagaactc gcctgggccg agcatggccg cccgcggcat tacgaccgcc agtccaagag
     3481 gcgccgacca caggacaggg cagaaggccc gatcctcgtg gtgccacgcc gtttcgttca
     3541 tattgttgag caggccgaac aggaaatcgc gccagcacct catcgaggga agcttcacgg
     3601 catatcgcct cgtgaggaga accaagcggt tcctcccctc ggtgacgacc ctcatcaggc
     3661 tgccccgatc gtgccggcct gctcggcccg ttgcacgaag aagcgctcgc cctggtcgat
     3721 cgagaagccg agctcggcga tcgcctcggc cgtcccgccc ttcagctgca gcagcttcag
     3781 cgtggctggt cgatcgagcg aatacttgag ccgggtggtc tgcttcccgt ggcgggtgcc
     3841 ccgcagcgcc tcgaccgcct tgtcgtcgct ctcgaatcca tggaccagct tgggcttcga
     3901 ggccctgctg ccgatcatgc acccgccgag ctcgatcgac ttgcgcttgc cggcgaggtc
     3961 cgggcctgcc gtcttccacc agccctcgag cttcagcaag atcgccccgg cctcggtgac
     4021 gaggggggcg gtgatcacgt cggccgcggc gttggcacga acaatgagcc ggctgcggcg
     4081 ttcctcagcg cgtgccagct cgccggtgat cgcggcgtag cgctcgagca tcgccgtcgc
     4141 ctgaccgacg gtacggggca cgcgcaccgt cacctttgcg ctcctgactg cagcgaatcg
     4201 atgacgtggg tcattcctgc gtgcatcgcg agctgggctt tggcggctcg gccttcagcg
     4261 atctccagcg ccacgcgggc gagccatttt ctgcagacga cggcgttcgg cgaatcggtg
     4321 cgggaggccg aacggcgcag tgcgtcgatg tcgagcggct cgcccatcac gatgtccagc
     4381 ggcttgtcca tcaccaggcg gccctgccgc ggccattgtc ccagaggggc gggttgacgg
     4441 cctcgggccc ggagcggccg cggaccagcg cgcgcaggtc gcccgcgatg gcttcggcct
     4501 cgtcgacgat gttgtcgtgg tcgcgctgcg agcgggaatc ctggctggcc atccattgca
     4561 gctggtcgaa gcggctgccg agggcacgca ggccctcgta gatggtgggc cgccgcccct
     4621 ggccggaccg gatctcggca ggggcggcgg cgggggcggc gggcctacgg cggccgcccg
     4681 ggggcgcgga gtgtgtggac atcgctgcgc tcccctcaaa cgcgaaaggc ccgccggggg
     4741 ggtggcgggc ctttcgaggt ttccatctct gcgtcgggcg cagttgtggc ggggtcgttt
     4801 tgaccggttc cgtggccgtt ttgcagtcgg cttttcgtca agttgacgaa aaccggacct
     4861 ttgccgcccc tagaggatcg ccgcttgggc ggcggccagg gtggctgcat cgatgtcctt
     4921 cgccacctgg cctaggatcc gcgaccacaa gtccaatgcc tcgaggagga gccgcttagc
     4981 cttccggttg tggatccggt atcgtttggc cacgacggtg aagccgaccg gctcgcccac
     5041 gatcatgtcc aggacggcgc cgatcggccc ctctacctgg ccccgccagc gcgtataggc
     5101 gacctcccgc cgaacttggc cgaggcgctc gtagaaggtg ccgtcgccca ttctggtcac
     5161 gtcgactcgg gtttccaggc tggcggtgcg gacggagaca tcggcgccga tcttctcggc
     5221 gacctgggcg atctcgaccc cggcggcgag ctgctcgccg ctgatcgtcc cgttctcgta
     5281 gagccgggcc agagcgccct ggttgcagcg ggaagcatgg tcgtgggttt ccggcgtgcc
     5341 ggcattctta tggctccaca ggtcgagcag ttcggcgcgc cccttccgga gcgaccgctc
     5401 ggccgccgcg gcctcggggt ggcggcgggc ccacaggatc ctgttggcct cctggtgctg
     5461 ttcgatcgcc cgctgcagct ccggccgccg atcgttcttg cggcgcttgc cggttggcgc
     5521 cggccgaagc gcgggtcgat tggccattcg ccgcctctcc gcgctctccc gcacccgaac
     5581 aaggcgcgcc tcgatcatgt ctgtcatgta accccgccca ggaaaccctg gcgcttcccc
     5641 ccggaagcgc cgtctcagcc cttgtaatcg agctccggca cgtcgggcag tccggtttgc
     5701 gtcaaggtcc gtcccaccgt cccctcgctt gcgttcacgc tccagcccag ctgcttgatc
     5761 ctcagcagca cgtccgcctc gctcagctgc tcgccgcgat cgatgagcac gatgccccgc
     5821 ctcttgccga cgacgcgctg gatctggtct tcgtcgcaga gttgctggac cagctcggag
     5881 gcgcgctgct tgctgacctc gagcgcggcg ccgatctcgt ccaggcttgg cgaatgcccc
     5941 cattcggcga agtatcgctt gatgaagtcg agcgcctgca gcttgcggct gcgcatcgtc
     6001 ggcttgaggc gacggttccc cgtcggatcg atgggaagag cttcgcccat ggcgcgactg
     6061 taacgcatca gcgatgaatg ggaacatagc aagactgagt tcagaagatt gtaggacttg
     6121 ccctcttcag tcctcagtca gcgcttgaat gggtccgacg ggaatcgctc ggcccgccga
     6181 ttggcctcgg cggccgccgc gacgcacgtc gccactcgcg ccatcgcccc ggcgctgccg
     6241 gtcagcttca tggcctcgag caccctctca ttgtcgtcgt caatcttcag ggccggagcc
     6301 agccgagctc ccttgatgat cgcttgcatg acctcaggtt tgagattgag gagaagcgtg
     6361 ccgccgctga ggcccaatcc tcggcccgaa aacaacttgt cgtccaccag caaatagagc
     6421 tcgtataact tattctcctc ggcactccag gcgtcgttat gaattatcat tttgaattgg
     6481 ccgtccaggc tttttccgag tgataggcgg gtaacgttgg tgtcgcggta aattgatgtg
     6541 accgccatgc agccgtcttc actttcctca atggaccatg catccgggct aggacgtgct
     6601 gccacgacgc tagatgcaag acttgagatc aagatcaacg cccgtgcgca actacgcact
     6661 cgagagcggc gaggcatcac catcggctcc cagcagcttc tcggcgctga ccagcgtcac
     6721 cgcccgccgg tgcggcgcca gccgccggaa aatcagcagt agctcgacct cgtcggccga
     6781 caatggcttg ttgcggtgga ccagaaacgc cgaccttcac c
//
LOCUS       DASWNU010000488         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_201402, whole genome shotgun sequence.
ACCESSION   DASWNU010000488 DASWNU010000000
VERSION     DASWNU010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201402"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..>1908)
                     /locus_tag="VGB57_09605"
     CDS             complement(223..>1908)
                     /locus_tag="VGB57_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY0131649.1"
                     /translation="LEELKIMKLDVLGLGMLGCMRRAFDLLRAHKGIDMNLWSDVLQE
                     DDPAVFAMIQKADTIGTFQIESRAQMSMLPRMKPKEFYDIVIQVAIVRPGPIQGDMVH
                     PYLRRREGKEKVEFPSPELKAVLGKTLGVPLFQEQAMNVAIVGAGFSPSDADRLRRGM
                     ATFKSTGGVSRFEDQMIEGMVARGYDRDFAERTFKQIEGFGSYGFPESHAASFAKIAY
                     ASSWMKCHHPDVFCAAILNAQPMGFYQPAQLVRDAQNHGVEVRPVCINESRWDTRMEE
                     GEGEEPSSRRRPGSQSGKGAALLTPRDPGFRRGDDLLPLRLGLRMVRGLSNLHAARIL
                     AARLDAPFASIEDVWRRSGVPVAALEKLADADAFASLGLDRRQALWRVRGLGEAPLPL
                     FAAADALGEEPEVALTPLTGGREVVEDYRTVQLSLRAHPLAFLREALGPKVATCAGLA
                     RLKDGSRVEVAGLVLIRQRPGKGNVTFITLEDETGIANVILWQRKFEAQRRIVMSAAM
                     IAVKGVMQKEGEVIHVVADRLEDLTPLLHRVGAMDAPAAAAAPVIKVKSRDFH"
BASE COUNT          308 a          644 c          651 g          305 t
ORIGIN      
        1 tcccccaccg ctacgcgccc gcggacggcg gcaagggcgt cccccccgac ccgcgcaagg
       61 cgaaggcaag ggaggtgatc aaggtgaaga cgagagactt tcattgagtc gaggaatcaa
      121 cccaagcaag tcattgcgag cgaagcgaag caatccagtg cagcctcgga ggcccgctgg
      181 attgcttcgt cgcttcgctc ctcgcaatga cggccctctt cctcaatgga agtccctcga
      241 cttcaccttg atcacgggcg cggcggcggc ggctggcgcg tccatcgcgc ctacccggtg
      301 cagcagcggc gtcagatcct cgagccggtc ggcgacgacg tggatgacct cgccttcctt
      361 ctgcatcacc cccttcaccg cgatcatcgc cgccgacatc acgatccgcc tctgcgcctc
      421 gaacttgcgc tgccacagga tgacgttggc gatccccgtt tcgtcctcca gggtgatgaa
      481 ggtgacgttg cccttgccgg gccgctggcg gatgaggacg aggcccgcca cctcgacccg
      541 gctgccgtcc ttgaggcggg cgaggccggc gcaggtcgcc accttcggcc ccagagcctc
      601 gcgcaggaag gcgagcggat gggcgcggag cgagagctgg acggtccggt agtcctcgac
      661 cacctcccgg ccgccggtga gcggagtcag ggccacttcg ggctcctcgc cgagggcgtc
      721 ggccgccgcg aacaaaggca agggcgcctc gcccagcccg cgcacccgcc acagagcctg
      781 gcgccggtcg agcccgagcg aggcgaaggc gtcggcgtcg gcgagcttct cgagcgccgc
      841 cacgggcact ccggagcggc gccagacgtc ctcgatcgaa gcgaaggggg cgtcgaggcg
      901 ggcggcgagg atccgggcgg cgtggaggtt ggagaggccc cggaccatgc gcagcccgag
      961 gcggaggggg agcaaatcgt caccccggcg aaagccgggg tcccgggggg tgaggagcgc
     1021 ggcgcctttt ccgctctggg atcccggcct tcgccgggat gacggttctt ccccctcccc
     1081 ctcctccatc ctggtatccc accggctctc gttgatgcac accgggcgca cctcgacccc
     1141 gtggttctgg gcgtcgcgga ccaattgggc gggctggtag aagcccatcg gctgggcgtt
     1201 caaaatggcc gcgcagaaga cgtcggggtg gtggcatttc atccagctcg acgcgtaggc
     1261 gatcttggcg aagctcgccg cgtggctttc cgggaagccg tagcttccga agccctcgat
     1321 ctgcttgaag gtgcgctcgg cgaagtcgcg gtcgtagccg cgcgccacca tgccctcgat
     1381 catctggtcc tcgaagcggc tgaccccgcc ggtcgacttg aaggtcgcca tcccgcggcg
     1441 aaggcggtcg gcgtcggagg gcgagaagcc ggcgccgacg atggcgacgt tcatcgcctg
     1501 ctcctggaag agcggcactc cgagcgtctt gccgagcacg gccttgagct ccggagaggg
     1561 gaattcgacc ttctccttgc cctcgcggcg gcggagatag ggatggacca tgtcgccctg
     1621 gatcggaccg ggacggacga tcgcgacctg aatgacgatg tcgtagaatt ccttgggctt
     1681 catccggggc agcatcgaca tctgggcccg gctctcgatc tggaaggtgc cgatcgtgtc
     1741 ggccttctgg atcatcgcga agaccgccgg atcgtcctcc tggaggacgt cagaccacag
     1801 gttcatgtcg atccccttgt gggcccgaag aagatcgaag gcccggcgca tgcagcccag
     1861 cattccgagg ccgagcacgt cgagcttcat gatcttcaat tcctcgag
//
LOCUS       DASWNU010000489         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_329666, whole genome shotgun sequence.
ACCESSION   DASWNU010000489 DASWNU010000000
VERSION     DASWNU010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329666"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..568)
                     /gene="dksA"
                     /locus_tag="VGB57_09610"
     CDS             complement(113..568)
                     /gene="dksA"
                     /locus_tag="VGB57_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY0131650.1"
                     /translation="MATALSDVYGSVYALGGVPESYRPSANEEFMNPVQLAYFRQKLL
                     GWKDEILRESQGTMASLKSGPIREADVTDRASSETDWAIELRTRDRQRKLISKIDSAL
                     RRIDTGEYGFCEVTGEPISLARLEARPIATMTVEAQERHERNERITRDD"
     gene            complement(1316..1765)
                     /locus_tag="VGB57_09615"
     CDS             complement(1316..1765)
                     /locus_tag="VGB57_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131651.1"
                     /translation="MLVLLEVLAEVLAEVEELVEVLVPPPKLDDDEVVDTLPLDEVVE
                     TPPVEVDVEPPLVEVDPPLVLVETPLLVDTPLLVELPPVDVLVDAVKMALPLEPPKKP
                     PLKKPPPKPPLPPKPPLPPITVTPPLPPVLPARGGSGGRGIGIDEPG"
     gene            complement(2132..2575)
                     /locus_tag="VGB57_09620"
     CDS             complement(2132..2575)
                     /locus_tag="VGB57_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment family protein"
                     /protein_id="HEY0131652.1"
                     /translation="MQVPHGSFVVVADGKKMLFFRNEGDSEYPKLEVERKREQENPAD
                     RDQRTDKPGRTFDASGGAGRSAYQETDFHQLEEERFAAETAELLKKRALRNDFDSLII
                     VAPPKTLGELRKHYHKEVEKRLSGEIAKDLTGHPVTEIEKIIQAE"
     gene            2666..3430
                     /gene="surE"
                     /locus_tag="VGB57_09625"
     CDS             2666..3430
                     /gene="surE"
                     /locus_tag="VGB57_09625"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0131653.1"
                     /translation="MRILLTNDDGVNASGLKVLEGIARRFSDDIWIVAPTEEQSGAGH
                     SLTLSRPVRLRKLGERRYCVTGTPTDAVMMAVAHLMKDCPPDLVLSGVNRGANLGEDV
                     TYSGTVSAAMEGAMAGFPAIALSQAYAKEGMGDSVPFAAAEAWAERVLRPLIEGGIPP
                     HTLVNINFPALPSGAVKGVRVAPQGRHDYARLRIVQRTDPRGYDYFWFGLAPMVETPG
                     HSTDLEVVADGYVAVTPLHFDLTHGASLDRLAELYG"
     gene            3466..>4208
                     /locus_tag="VGB57_09630"
     CDS             3466..>4208
                     /locus_tag="VGB57_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY0131654.1"
                     /translation="MRRRGRLPVWAQLAWRAALVVGLLAFTIGLHWFERDGLKDTHDG
                     HISFADVVYFTMISITTTGYGDIVPVAPHTRLYDALVVTPIRIFFVLIFIGTAYTFVF
                     RRTWDKWRMKRLQRTLCDHVVVAGFGTSGSEAVDELIARGTKPDEIVVIDAVEDALER
                     AESLGCAIVDADATRDKTLQAVHIQRARAMIVTAGRDDTSILITLTARHLAPDLTISV
                     AVRNEDNELLARQAGATTVINPVSFAGLLL"
BASE COUNT          671 a         1488 c         1297 g          752 t
ORIGIN      
        1 cccagcgaag gcagggaccc cagcgggtga agggctcggc ggatttcacc ccacggagcc
       61 cagccttcgc tggggtcacg cgccggccgg aagagcccgc ttccggcctt cgtcaatcgt
      121 cgcgggtgat gcgctcgttg cgctcgtggc gctcctgggc ctcgaccgtc atcgtcgcga
      181 tcggccgggc ctcgagacgc gccagcgaga tcggctcgcc ggtcacttcg caaaagccgt
      241 attcgccggt gtcgattcgg cggagcgccg agtcgatctt cgagatgagc ttgcgctgcc
      301 ggtcgcgggt cctgagctcg atcgcccagt cggtctccga ggaagcgcgg tcggtgacgt
      361 ccgcctcgcg gatcgggccg ctcttcagcg acgccatcgt cccttgcgat tcgcgcagga
      421 tctcgtcctt ccagccgagc agcttctgcc ggaaataggc gagctgcacg ggattcatga
      481 actcttcgtt ggcactgggc cgatagcttt cgggcactcc gcccaaagca tagaccgagc
      541 cgtaaacgtc actcaaagcc gtcgccataa cccctccaca tccttcacgc tcccgccatg
      601 cggtcacgca aacctcctgc agctccgctt ccgagtcgtc cggaaatgtc ggagcttgcc
      661 ccagcgcgcc catacgcttc gcaaaggagg agtacaagtc actcaaatag cgcttaaaaa
      721 ctcacccgga ttcggacagg ggtagtgact tttttgcccg tttcggcgcc tcgccccgat
      781 tcgccggcgc cggctccgaa aatcgcgaat cgggatggcc gcggcgccgt taaccatgtt
      841 cgtccgatcc gtcccgtcgc ctattcgttc acggcgcgtc cgggtctgtc ctcaaatggg
      901 acggaagtcg cccgaacggc catttggcgg gcattccgag gggtcttcgt ggttaacgcg
      961 cttgcactta aagctttcct ttgcatctgt gcatgtagcc cgaggctgaa gccgatcttc
     1021 caccaggact ttcggcgacg catcggaacc tgaatataag gtgcgctgtc atgtcggtta
     1081 gaatggctct cgctaaactc tgcgcctgca cttgcggcgg cgcgatcatc ggtggcggcg
     1141 cagtccatgt cgccgagaac ccgccccagc ggccggcctt cgtcaagcag gccaaaatgg
     1201 tcaagcaggc cccgcggcgc cacgcccggg tcaaggcgat gaagcgctac aagagggtgc
     1261 gcacggcctg cgtcgtaaag accccgggca cgaccaccac gacccggacc atcacctatc
     1321 ccggttcgtc gatgccgatc cccctgcccc cgcttccgcc cctggctgga agcaccggcg
     1381 gcagcggcgg agtgaccgtg atcggcggca gcggcggctt cggcggcagc ggcggcttcg
     1441 gcggaggctt cttcagcggc ggcttcttcg gcggttcgag cggcaacgcc atcttcacgg
     1501 cgtcgaccag cacgtcgacc ggcggaagct cgacgagcag cggcgtgtcg accagcagcg
     1561 gcgtctcaac gagcaccagc ggcggatcga cctccaccag cggcggctcc acgtcgacct
     1621 cgacgggcgg agtctcgacc acttcgtcga gcggcaacgt gtcgaccacc tcgtcgtcgt
     1681 cgagcttcgg cggcggcacc agcacctcga ccagctcctc gacctcggcc agcacctccg
     1741 ccagcacttc gagcagcacc agcacgtcga ccagcgcctc gagctcgggc agcagctcga
     1801 cctcgagcgg cggccacgac gttccggcgc cgccgatgat ccttctcttc ggagcggcgg
     1861 ccgcggccct ggtcgcccgc cgcaggattg cccgcgccgg agactagccc ggccggacaa
     1921 cgaaaaggcc gcggggtccc ttcgccccgc ggccttttcc acgtctggag gagatgggac
     1981 ggatcggcgc gtcgcgccaa tccgtcaccc cggcgaaagc cggggcctca ggtcggaaaa
     2041 gctcggcggg tctctccgag gtcccggctt tcgggttccc atcaaagtac tactttgatg
     2101 gggtcctccc gggatgacga ttgagccccg cctattccgc ctgaatgatt ttctcgatct
     2161 ccgtgaccgg atggccggtc aggtccttgg cgatctcgcc cgacaggcgc ttctcgacct
     2221 ccttgtgata atgcttcctg agctcgccga gagtcttggg cggggcgacg atgatcaggg
     2281 aatcgaagtc gttgcgaagc gcccgcttct tcagcagctc ggccgtctcg gccgcgaagc
     2341 gctcctcctc gagctggtgg aaatcggttt cctgataggc gcttcgcccc gccccgcccg
     2401 aggcgtcgaa ggtgcgcccg ggcttgtcgg tcctctggtc ccggtcggcg ggattttcct
     2461 gctcgcgctt ccgctcgacc tcgagcttgg gatattcgga atcgccctcg ttgcggaaga
     2521 acagcatctt cttgccgtcg gcaacgacca cgaaactacc gtgcggaacc tgcatcgctt
     2581 tctccttctt cgcccgacca actcccgaat cgcggcgatg gttgccacgc ttgcgccgcc
     2641 gccgccgccg ctttagctcg gtgcgatgcg catcctcctc accaacgacg acggagtgaa
     2701 tgccagcggc ctcaaggtgc tggaggggat cgcgcgccgc ttttccgacg acatctggat
     2761 cgtcgccccg accgaggagc aatcgggcgc gggccattcg ctcaccctct cccgtccggt
     2821 acgcctgagg aagctcggcg agcgccgcta ctgcgtcacc ggaacgccca ccgacgcggt
     2881 gatgatggcg gtcgcgcatc tgatgaagga ctgcccgccc gacctcgtcc tctccggagt
     2941 gaatcgcggc gccaatctcg gcgaggacgt cacttattcg ggcaccgtct cggcggcgat
     3001 ggaaggcgcg atggccggct ttccggcgat cgctctcagc caggcctatg ccaaggaagg
     3061 catgggcgac tcggtgccct tcgccgccgc cgaagcctgg gcggaacggg tgcttcggcc
     3121 tctgatcgaa ggcggcattc cgccgcatac cctcgtcaac atcaatttcc ccgctttgcc
     3181 gtcgggcgcc gtaaaggggg tgagggtcgc gccccagggc cggcacgatt atgcccggct
     3241 tcgaatcgtc cagcgcaccg acccgcgtgg ctacgattat ttctggttcg gcctagctcc
     3301 gatggtcgag acaccgggcc attcaaccga tctcgaagtg gtcgccgacg gctatgtcgc
     3361 cgtcactccg cttcatttcg acctgaccca cggcgcgtcg ctcgatcgcc tggcggagct
     3421 ttacggctga tgcgccggcg ccaccgcggt cccgacgaga tcaacatgcg ccgccgtggc
     3481 cgactgccgg tctgggcgca gctcgcctgg cgggcggccc tcgtcgtcgg gctgctcgcc
     3541 ttcaccatcg gcctgcactg gttcgagcgc gacggcctca aggatacgca cgacggccat
     3601 atcagcttcg ccgacgtcgt ctatttcacg atgatcagca tcaccacgac cggctatggc
     3661 gacatcgttc cggtcgctcc gcacacccgc ctctacgacg ctctggtggt gaccccgatc
     3721 cggatcttct tcgtcctcat cttcatcggc accgcctaca cgttcgtgtt caggcgcacc
     3781 tgggacaaat ggcgtatgaa aaggcttcag cggactttgt gcgatcatgt cgtcgtcgcc
     3841 ggcttcggca ccagcggctc ggaagcggtg gacgagctca tcgcccgcgg aaccaagccg
     3901 gacgagatcg tcgtcatcga cgcggtcgag gatgcgctcg agcgggccga atcgctgggc
     3961 tgcgcgatcg tcgacgccga cgcgacccgc gacaagaccc tccaggccgt ccacatccag
     4021 cgggcccggg cgatgatcgt caccgccggc cgcgacgata cctcgatcct catcaccctc
     4081 accgcccgcc acctcgcgcc cgacctgacg atcagcgtcg cggtcaggaa cgaggacaac
     4141 gaactgctcg cccgccaggc cggcgccacc acggtgatca acccggtgag cttcgccggc
     4201 ctgctgct
//
LOCUS       DASWNU010000490         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_459551, whole genome shotgun sequence.
ACCESSION   DASWNU010000490 DASWNU010000000
VERSION     DASWNU010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459551"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGB57_09635"
     CDS             complement(<1..460)
                     /locus_tag="VGB57_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0131655.1"
                     /translation="MNQAHANHETHGSLTTRAALASVTTALFLLVLKGYAAWTTGSIA
                     MLGSLADTGLDLLASLITLYGVRVAAAPADQEHRFGHGKAEALAALAQVGIIAFSALG
                     IGWRAVDRLIRGAPTEEAEYGIAVSVVAILTTFALLAYQRFVIRRTGSVAI"
     gene            complement(457..1035)
                     /gene="pdxH"
                     /locus_tag="VGB57_09640"
     CDS             complement(457..1035)
                     /gene="pdxH"
                     /locus_tag="VGB57_09640"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY0131656.1"
                     /translation="MAIDPHALFEEWLAEARLGEPNDPTAMALATADADGRPSVRMVL
                     MKGHDERGFVFYTNFDSRKGEELAANPAAGLLFHWKSLRRQVRVEGPVEPVSDEEADA
                     YFATRGRDSQLGAWASDQSRPLDARATFEARYEEVRRRFEGGDVPRPPRWGGWRVAPE
                     RIEFWSDRAHRLHERRLFTRAGEGWSEGLLYP"
     gene            1274..2245
                     /locus_tag="VGB57_09645"
     CDS             1274..2245
                     /locus_tag="VGB57_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY0131657.1"
                     /translation="MADLYSQLGVKRDSGEAEIKKAYRKLAKELHPDKNKGNPNASER
                     FSKVTRAYDILTDKDKRARYDRGELDEDGNPRAPFGYGGGPRPGEGPGGAGFGRTPGP
                     DFGGGEEIDLSDLFDGLFSGYAGQRRGAPGGFGGFGGFGTRRPAPPPQKGADTAYRLP
                     ISFEDAAALKEQRVTLAGGKTVSIKLPAGVEEGTKIRLAGQGQAGPGGNGDAIVAISI
                     KPHRFFTRDGDNIRLDLPVSLDEAVLGAKVKVPTVDGPVMVSVPKGSSSGKTLRLKGR
                     GFTGKNGVRGDQLVTLMVEIPDDAELARFVEGWSRKGLGNPRAGLGV"
     gene            2342..3271
                     /locus_tag="VGB57_09650"
     CDS             2342..3271
                     /locus_tag="VGB57_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0131658.1"
                     /translation="MKPGQYPFEVIKRVAIGTYNDGFIHAGNLAYLALMTLFPFVIVA
                     AAIARLFGQTEGGMQAVVALFQTMPPDVADVLRKPINDVLQARSGNLLWLGALVGLWT
                     TGSFIETIRDILRRAYGVTLGAPFWEYRLASIGMIFASVMMMLVAFSATILLSSVTQF
                     IERVIPRADDVAGMLALSRVVPGLVLFGSIYLIFVSLTPRRYRNRQFRKWPGALLVTL
                     WWIATTALLPVVLAGLGGYDLTYGSLAGVIIALLFFFVIGLGVVIGAQLNAALAEPPE
                     PTVQEAEVAVAEAALEEAAAAELEAVAEGETKA"
     gene            3268..>4050
                     /gene="fabI"
                     /locus_tag="VGB57_09655"
     CDS             3268..>4050
                     /gene="fabI"
                     /locus_tag="VGB57_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0131659.1"
                     /translation="MTGLMAGKRGLIMGLANDKSLAWGISKRLAEHGAELAFSYQGEA
                     LQKRVGPLAASVGSDFLIECDVADMADLDRLFEQLKTRWETIDFVVHAIGFSDKNELR
                     GKYVDTSLDNFLMSMNISAYSFTAVARRAREMMPDGGALLTLTYYGAEKVIPHYNVMG
                     IAKAALETSVKYLAMDLGPESIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPLRR
                     NVTIEDVGGAGLYLLSDLASGVTGEIHHVDAGYNVVGMKAEDA"
BASE COUNT          667 a         1364 c         1365 g          654 t
ORIGIN      
        1 ggatcgcgac cgatccggtg cggcggatga cgaagcgctg gtaggcgaga agcgcgaagg
       61 tggtgaggat cgcgaccacc gagacggcga tgccgtactc cgcttcctcg gtcggtgcgc
      121 cacggatcag ccggtcgacc gcgcgccagc cgatgccgag cgccgagaaa gcgatgattc
      181 cgacctgggc cagcgccgcc agcgcctccg ccttgccgtg gccgaagcgg tgctcctggt
      241 cggcgggcgc cgcggccacc cggactccgt agagcgtgat cagcgaggcg agcaggtcga
      301 gcccggtatc ggcgagcgag ccgagcatgg cgatggagcc ggtggtccag gccgcatagc
      361 ccttcagcac aagcaggaag agggccgtgg tgacgctggc cagcgccgcc cgggtggtca
      421 gcgacccgtg cgtttcgtga ttcgcgtgag cctggttcac gggtagagca gcccctcgct
      481 ccagccttcg ccggcgcggg tgaaaagacg gcgctcgtgc aggcggtggg cgcggtcgct
      541 ccagaactcg atccgctccg gcgccacccg ccagccgccc cagcgcggcg gccgaggcac
      601 gtcgccgccc tcgaaccggc gccggacctc ttcgtagcgc gcttcgaagg tcgcccgggc
      661 gtcgagcggt cgcgattggt ccgacgccca ggcgccgagc tgcgaatcgc ggcctcgagt
      721 ggcgaaatag gcgtcggcct cctcgtcgct gaccggctcg accggtcctt cgacacgcac
      781 ctggcggcgc agcgacttcc agtggaagag caggcccgca gcggggttgg cggcaagctc
      841 ctcccccttg cggctgtcga aattggtgta gaagacgaag ccgcgctcgt cgtggccctt
      901 catcagcacc atccgcaccg aggggcggcc atcggcatcg gccgtggcga gcgccatggc
      961 cgtcggatcg ttcggctcgc ccagccgcgc ctcggcgagc cattcctcga acaatgcgtg
     1021 ggggtcgatg gccatcgccg cctcttgccg tgtgagaggc tatggcacaa gcgtacgccc
     1081 acttccgtca tcccggcgaa ggccgggatc catgaacacg tatggacgag ttaaatcgcg
     1141 gcggtgttca tcgattccgg ccttcgccgg aatgacgggg aggttgcacc cttccggtgc
     1201 gttacccatc tgacacagcg tatcgacaac gcaacagtgg acatttgcgg cggtctggag
     1261 aacgagcagc aggatggcgg acctctattc acagctgggc gtgaagcgcg actcgggcga
     1321 ggccgagatc aagaaggcct atcgcaagct cgccaaggag cttcatcccg acaagaacaa
     1381 gggcaatccc aacgcctcgg agcgcttctc caaggtaacg cgcgcctatg acatcctcac
     1441 cgacaaggac aagcgggccc gctacgaccg cggcgagctg gacgaggacg gcaatccgcg
     1501 cgcgcccttc ggctatggcg gcgggccccg tccgggcgag ggtccgggcg gagcgggctt
     1561 cggccggacg ccgggccccg atttcggcgg cggcgaggag atcgacctgt ccgacctgtt
     1621 cgacggcctg ttcagcggct atgccggcca gcgccgcggc gcgcccggcg gcttcggcgg
     1681 gttcggcggg ttcggcaccc gacgccccgc gcctcctccc cagaaagggg cggataccgc
     1741 ctatcgtctg ccgatcagct tcgaggacgc cgcggccctc aaggagcagc gggtcaccct
     1801 cgccggcggc aagacggtgt cgatcaagct tccggccggc gtagaggaag ggaccaagat
     1861 ccgcctcgcc ggccagggcc aggccgggcc cggcggcaat ggcgacgcga tcgtcgccat
     1921 ctcgatcaag ccgcaccgct tcttcacccg ggacggcgac aatatccggc tcgatctgcc
     1981 cgtctcgctc gacgaggccg tgctcggcgc caaggtcaag gtgccgaccg tcgacgggcc
     2041 ggtgatggtg agcgtcccca agggatcttc gtccggcaag acgcttcgcc tcaagggccg
     2101 gggcttcacg ggcaagaacg gcgtccgggg cgaccagctg gtgacgttga tggtggaaat
     2161 ccccgacgat gccgagctcg cgcgcttcgt cgagggctgg agccggaagg gcctaggaaa
     2221 cccgcgggcg gggctgggcg tctagccctt cgtgcaaacg acaatatccc ccgaatcgcc
     2281 ggaagccagg cgtaagcgcc tcgccaggac caaggcccgc ttcggcaaga tccgtcacgg
     2341 aatgaagccg ggccaatatc cgttcgaagt gatcaagcgg gtcgccatcg gcacttacaa
     2401 cgacggcttc atccacgccg gcaaccttgc ctatctggcg ctgatgactc tgtttccctt
     2461 cgtcatcgtg gcggccgcga tcgctcgcct gttcgggcag acggaagggg gcatgcaggc
     2521 tgtcgtcgcg ctgttccaga cgatgccgcc cgacgtggcg gacgtgctca ggaagccgat
     2581 caacgacgtg ctccaggcgc gttcgggcaa tctcctctgg ctcggcgccc tggtcgggtt
     2641 gtggaccacc ggcagcttca tcgagacgat ccgggatatc cttcgccgcg cctacggggt
     2701 gacgctcggc gcgcccttct gggaatatcg cctggcctcg atcggaatga tcttcgcgtc
     2761 ggtcatgatg atgctcgtcg cgttcagcgc caccatcctt ctttccagcg tcacccaatt
     2821 catcgagcgg gtgattccgc gggcggacga cgtggcgggg atgctcgctt tgtcgcgggt
     2881 ggtgccgggg ctggtcctgt tcggctccat ctacctcatc ttcgtctccc tgactccgcg
     2941 gcgctaccgc aaccgccagt tccgcaagtg gcccggggca ctgctcgtta cattgtggtg
     3001 gatcgccacc accgccttgc tgccggtggt gctggccggc ctcggcggct acgatctcac
     3061 ctacggcagc ctcgccggag tcatcatcgc cctgcttttc ttcttcgtga taggcctcgg
     3121 cgtggttatc ggcgctcagc tcaatgcggc gctggcggaa cctccggagc ctacggtaca
     3181 ggaagccgag gtggccgtgg cggaggcggc cctggaggag gcggcggccg ccgagctgga
     3241 agcggtcgcg gaaggggaga ctaaggcatg accgggttga tggcgggcaa gcgcgggctg
     3301 atcatgggcc tcgccaacga taagtcgctc gcctggggga tttcgaaacg gctggccgag
     3361 cacggcgccg agctcgcttt ctcctatcag ggcgaggcgc ttcagaagcg ggtgggcccg
     3421 ctcgccgcct cggtgggctc cgatttcctg atcgaatgcg acgtcgccga catggccgat
     3481 ctggaccggc tgttcgagca gctcaagact cgctgggaga cgatcgactt cgtcgtccac
     3541 gccatcggct tctccgacaa gaacgagctt cgcggcaaat atgtcgatac cagcctcgac
     3601 aatttcctga tgtcgatgaa catctccgcc tacagcttca ccgccgtcgc ccgccgcgcc
     3661 cgcgagatga tgcccgatgg cggagcgctc ctcaccctga cctattacgg cgccgagaag
     3721 gtcatccccc attacaacgt catgggcatc gccaaggcgg cgctggagac cagcgtcaag
     3781 tatctcgcca tggacctcgg ccccgagagc atccgggtca acgccatctc cgcgggcccg
     3841 atcaagacgc tcgcggcaag cggcatcggc gacttccgct acatcctgaa gtggaatgaa
     3901 tataacagcc cgctgcgccg caacgtcacc atcgaggacg tcggcggcgc cggcctctac
     3961 ctcctctccg acctcgcctc gggcgtcacc ggcgagatcc accacgtcga cgccggctac
     4021 aacgtggtgg ggatgaaggc ggaggacgcg
//
LOCUS       DASWNU010000491         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_329702, whole genome shotgun sequence.
ACCESSION   DASWNU010000491 DASWNU010000000
VERSION     DASWNU010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329702"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..866)
                     /locus_tag="VGB57_09660"
     CDS             complement(183..866)
                     /locus_tag="VGB57_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY0131660.1"
                     /translation="MRIAVLEAGRPPGDLAARFGTYPDMYRQMLGLSEPLPAYDVAAG
                     ELPERPEAHDAYIVSGSPAGVYEPLPWIAGLLAFLRQARGRARLVGICFGHQAMAQAF
                     GGRVAKSDKGWGVGLHESAVVAREPWMDAALSVSIPASHQDQVVEQPPGTAVTVASGF
                     TPIAGLAWRDHPSVSFQFHPEFSPAFAKALLETRRERVPDADSALASLDRFNDNERVG
                     KWIAKFLAN"
     gene            complement(955..2220)
                     /locus_tag="VGB57_09665"
     CDS             complement(955..2220)
                     /locus_tag="VGB57_09665"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY0131661.1"
                     /translation="MTEAPRKVVLAYSGGLDTSIILKWLQTEYRAEVVTFTADLGQGE
                     EVEPARRKAELLGIKPENIFIEDVREEFVRDYVFPMFRANTVYEGQYLLGTSIARPLI
                     AKRQIEIARAVGADAVCHGATGKGNDQVRFELGYYALEPDIRVIAPWREWEYASREQL
                     IDFAEKNQIPIAKDKRGESPFSIDANLLHSSSEGKVLEDPSVEAPEYVHQRTISPEDA
                     PDAPTVFTIDFAAGDPVAVDGEALSPAALLTRLNQLGHDNGIGRLDLVENRFVGMKSR
                     GVYETPGGTILLAAHRGIESITLDGGAMHLKDEIMPRYAKLIYNGFWFSPEREMLQAL
                     IDKSQEHVTGRVTMKLYKGNATVIGRESPLALYDQDLVTFEEGSSSYSQRDAAGFIRL
                     NALRLRTLATRDRRAAEAAGSHPGESRDR"
BASE COUNT          382 a          803 c          770 g          400 t
ORIGIN      
        1 agcagcaagc cgaccagtcc gccccgcgtg agccttgtga ccgccccgtt gaagaggtga
       61 attcgtgcca tggtgtcccc cgaccccgcc ggccaactcg ccgagtctcc ggccatggac
      121 tagacaagtc accggcatcg ggagtcaagc aagaggcggc gcggcgatcc tgggttccgc
      181 cgctagttcg ccaggaactt cgcgatccac ttgccgacac gctcattgtc gttgaaccgg
      241 tcgagcgagg ccagggcgct gtccgcgtcc ggcacccgct cccggcgcgt ctccagcagc
      301 gccttggcga aggcgggcga gaattccggg tgaaactgga aggagacgga cggatggtcc
      361 cgccacgcca gcccggcgat cggggtaaag ccgctcgcca ccgtcaccgc cgtgccgggg
      421 ggctgctcga ccacctggtc ctggtgcgag gcggggatcg agaccgaaag cgccgcgtcc
      481 atccacggct cgcgcgccac caccgcagat tcgtggaggc cgacgcccca gcccttgtcc
      541 gacttggcga cgcggccgcc gaacgcctgc gccatcgcct ggtgaccgaa gcagatgccg
      601 accagccggg cgcgcccgcg cgcctgccgg aggaatgcga gcaggccggc gatccacggc
      661 agcggctcgt acacgcccgc cggcgagccg ctgacgatat aggcatcatg cgcctccggc
      721 cgctccggca gctcgccagc ggcgacgtcg taggccggca gcggctcgct tagcccgagc
      781 atctgccggt acatgtccgg ataggtcccg aagcgcgcgg cgagatcgcc cggcggccgg
      841 cctgcttcca gaaccgcgat cctcattcca ccactcttga gtcaccccgg cgaaagcctg
      901 ggcccatgaa caccgcaggc ggtcgaatct gcgccaagtc tgcggccagg cggctcatcg
      961 gtcccggctt tcgccgggat gactgcccgc cgcttccgcc gccctccggt ccctggtcgc
     1021 aagcgtgcgc agccgcaggg cgttgaggcg gatgaagccg gccgcgtcgc gctggctgta
     1081 gctcgacgag ccttcctcga aggtaaccaa gtcctggtcg tagagcgcaa gcgggctctc
     1141 ccggccgatc accgtcgcat tgcccttgta gagcttcatc gtcacccgtc cggtgacatg
     1201 ctcctggctc ttgtcgatca gcgcctggag catctcgcgc tccggcgaga accagaagcc
     1261 gttatagatg agcttggcgt agcgcggcat gatctcgtcc ttgagatgca tggcgccgcc
     1321 atccaaggtg atgctctcga tgccgcggtg cgccgccagc aggatggtgc cgccgggggt
     1381 ctcgtagacg ccgcgcgact tcatgccgac gaagcggttc tcgaccaggt ccaggcggcc
     1441 gatgccgttg tcgtgaccga gctggttgag gcgggtgagg agggcggcgg gcgacagggc
     1501 ctcgccgtcc accgccaccg ggtcgccagc cgcgaagtcg atcgtgaaca cagtcggcgc
     1561 gtccggcgcg tcctcgggcg agatggtgcg ctggtggaca tattccggcg cctcgaccga
     1621 cgggtcctcc agcacctttc cctcgctcga cgagtggagc aggttcgcgt cgatggagaa
     1681 ggggctctcg ccgcgtttgt ccttggctat cgggatctgg ttcttctcgg caaagtcgat
     1741 cagctgctca cggctggcat attcccattc gcgccagggc gcgatcaccc ggatgtccgg
     1801 ctccagcgca taataaccga gctcgaaccg cacctggtcg ttgcccttgc cggtggcgcc
     1861 atgacagacc gcgtcggcgc cgaccgcccg tgcgatctcg atctggcgct tggcgatgag
     1921 cggtcgggcg atcgaggtgc ccagcagata ttgtccctcg tagaccgtgt tcgcccggaa
     1981 catcgggaag acatagtcgc ggacgaactc ctcgcgcaca tcttcgatga agatattctc
     2041 cggcttgatc ccgagcaatt cggccttgcg ccgcgccggc tcgacctcct caccctggcc
     2101 gagatcggcg gtgaaggtga cgacctcggc gcgatattcg gtctgcagcc atttgaggat
     2161 gatcgaggtg tcgagcccgc ccgaataagc gaggacgacc ttcctgggtg cttcagtcat
     2221 gtttcagtcc cgaattctaa gaacccgctc accctgagct tgtcgaaggg ctgtccttct
     2281 cctcggaagg aagggcaggg cttcgacagg ctcagcctga gcggtggtgg gtaacaagcc
     2341 tcatgcataa acctt
//
LOCUS       DASWNU010000492         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_304446, whole genome shotgun sequence.
ACCESSION   DASWNU010000492 DASWNU010000000
VERSION     DASWNU010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304446"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGB57_09670"
     CDS             complement(<1..365)
                     /locus_tag="VGB57_09670"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0131662.1"
                     /translation="MSDARELLGVRLSSPDKVLYEEQGITKSELADYYIAVAARMLPH
                     VAGRPVTLVRCPTGAEKKCFYQRHAASGVLSQLKEVTVPGFDEPYLYIEDEAGLVAAV
                     QMGTLELHPWGVTVERPDRP"
     gene            complement(362..1402)
                     /locus_tag="VGB57_09675"
     CDS             complement(362..1402)
                     /locus_tag="VGB57_09675"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0131663.1"
                     /translation="MPAFANLPLRPTIPPMEAKLVEALPEGEGWHHEPKWDGFRVLVF
                     RDGEDVELQSKAEKPLGRYFPELVEAVRAIPRERFVVDGEMVIPVNGELSFDALQMRL
                     HPAESRIRKLARDTPARIILFDCLALDDRPLVAEPLGTRRQALEEFHGAAGGDLMLSP
                     YTRDRAVAEGWLAGSGVALDGVIAKRADAPYSPGERTMLKVKRLRTADCVVGGFRYAT
                     GKREVGSLLLGLYDDQGKLNHVGFTSAIPAAERPALTRRLEALVEAPGFTGSAPGGPS
                     RWSTERSTEWQPLRPELVVEVRFDHAGANRFRHGTGFLRWRPDKAPRQCRMEQLYSPT
                     PLAAIGDLGGGA"
     gene            1569..>2456
                     /locus_tag="VGB57_09680"
     CDS             1569..>2456
                     /locus_tag="VGB57_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131664.1"
                     /translation="MEQDKGIFPTFFLSGFECSTFIWKDQGRRDLCEETQHWSHADED
                     YRMLHDLGIGVAREGIPWPLVDRGGTLDFSCIDPFLDAQRRHDILPIWDLCHYGYPDD
                     LDPFGADFPLRFAAYAKAAAEYVVAGAHHGPHFFTPINEITFFGYMAGEWAWAAPFGK
                     DKETRRRFTLALCEADIAAVKAIRSAVPEARMVHIDPLILVVPPRDRPDLADEAWRET
                     YDDAFFAFDVIGGLRHPEAGGAPELLDIVGFNNYSFGQMEYRPDGPHAALEPGDPRIR
                     PLCDLIEEGWKRYHRPVIVA"
BASE COUNT          383 a          880 c          824 g          369 t
ORIGIN      
        1 gggcggtccg ggcgctcgac tgtaacgccc catggatgga gctccagcgt ccccatctgc
       61 acggccgcga ccagccccgc ttcgtcttcg atatagagat aaggctcgtc gaagccgggg
      121 accgtgacct ccttcaactg cgacagcacg cccgaggcgg cgtggcgctg gtagaagcac
      181 ttcttctccg ctccggtcgg gcagcgcacc aaggtcaccg gccggccggc gacgtgcggc
      241 agcatccggg ccgcgacggc gatataataa tcggcaagct cgctcttggt gatgccctgc
      301 tcctcgtaga gcaccttgtc ggggctgctg agccggactc cgagcagctc gcgcgcatcg
      361 ctcacgcccc gcctcccaaa tcgccgatcg ccgccagcgg agtcggcgaa tagagctgct
      421 ccatccggca ctgccgcggc gccttgtcgg gacgccagcg caggaagccg gtgccgtggc
      481 ggaagcggtt cgcgccggca tggtcgaagc ggacctcgac caccagctcg ggccgcagcg
      541 gctgccactc ggtcgaacgc tcggtcgacc agcggctcgg gccgcccggg gccgagccgg
      601 tgaagcccgg cgcctcgacc agggcctcga gccgcctggt cagcgccggc cgctcggcgg
      661 cggggatggc ggaggtgaag ccgacatggt tgagcttgcc ctggtcgtca tagaggccca
      721 gcaacagcga gccgacctcg cgctttcctg tagcgtagcg gaagcccccc accacgcagt
      781 ccgcagtgcg caggcgcttg accttcagca tcgtgcgctc gcccggcgaa taaggagcat
      841 cggcgcgctt ggcgatcacc ccgtcgagcg cgacgcccga tccggcgagc cagccctctg
      901 ccaccgcgcg gtcccgcgtg tacggcgaca gcatcagatc gccccccgcc gccccgtgga
      961 attcctcgag cgcctgccgc cgggtgccga gcggctcggc cacgagcggg cgatcgtcaa
     1021 gcgccaggca gtcgaacagg atgatccgcg ccggcgtgtc ccgcgccagc ttgcggatgc
     1081 ggctctcggc cgggtggagc cgcatctgca gcgcgtcgaa gctgagctcg ccgttcaccg
     1141 ggatcaccat ctccccgtcg accacgaaac gctcgcgggg gatcgcccgc accgcctcga
     1201 ccagctccgg gaaatagcgg ccgagcggct tctcggcctt ggactggagt tcgacatcct
     1261 cgccgtcgcg gaagacgagg acccggaacc cgtcccattt gggctcgtgg tgccagccct
     1321 ccccttccgg cagcgcctcg accagcttgg cctccatcgg ggggatggtg gggcgcaggg
     1381 ggagattggc gaaagcgggc acgggatcga aacgacgcga gcggagccgc tgttcccgcc
     1441 gccgcgagtc cagcgaagag cgccgggcgg ccagagggga cccggacccc gggctgccgg
     1501 aacctgcctt ctcccccgcc aggtcccttg caacggaccc ccgggatgca gccgcgcgta
     1561 gtgaggggat ggaacaggac aaaggcatct tcccgacctt cttcctctcc gggttcgaat
     1621 gctcgacctt catctggaag gaccaggggc ggcgggacct gtgcgaggag actcagcact
     1681 ggagccatgc cgacgaggat taccggatgc tccacgatct gggcatcggc gtcgcccgcg
     1741 agggcatacc ctggccgctc gtcgatcgcg gcggaacgct ggacttcagc tgcatcgacc
     1801 cgttcctcga tgcacagcgg cgtcacgaca tcctgccgat ctgggacctg tgccattacg
     1861 gctatcccga cgacctcgat ccgttcggcg ccgacttccc gctccgcttc gccgcttatg
     1921 ccaaggcggc ggccgaatat gtggtcgccg gggcgcatca cggcccgcac ttcttcaccc
     1981 cgatcaacga gatcaccttc ttcggctaca tggccgggga atgggcctgg gccgctccgt
     2041 tcggcaagga taaggagacg cggcgccgct tcaccttggc gttgtgcgag gccgacatcg
     2101 cggcggtgaa ggcgatccgg tcggccgtgc ccgaagcgcg catggtccat atcgatcctc
     2161 tcatcctggt cgttccgccc agggaccggc cggacctggc cgacgaggcg tggcgcgaga
     2221 cctatgacga cgccttcttc gccttcgacg tgatcggcgg cctgcgccac ccggaggcgg
     2281 gcggagcgcc cgagctgctc gatatcgtcg gcttcaacaa ttacagcttc ggccagatgg
     2341 aatatcgccc cgacgggccg cacgcggcgc tggagcccgg cgacccgcgg atccggccgc
     2401 tctgcgacct catcgaggaa ggctggaagc gctaccaccg gccggtgatc gtcgcc
//
LOCUS       DASWNU010000493         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_97219, whole genome shotgun sequence.
ACCESSION   DASWNU010000493 DASWNU010000000
VERSION     DASWNU010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97219"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..772
                     /locus_tag="VGB57_09685"
     CDS             134..772
                     /locus_tag="VGB57_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0131665.1"
                     /translation="MEETKAEEKKRNVTICTVLKHPLRVRILEVLNEGPRSPSQFVEE
                     GLIPKEHYSTYQQALSLASYHFRELEKEGCLEVVESIPRRGAVEHVYRGMSPQVFFSD
                     AEFEAMSLETRRQLTRISLQGLIARADRAISTETFDARSDRHLTWVPMQLDERGWSEV
                     IAALAATFGELTQIRHDAKDRLAASGEEVVPATVAMLGFESPPPPPLTRSDD"
     gene            812..>1775
                     /locus_tag="VGB57_09690"
     CDS             812..>1775
                     /locus_tag="VGB57_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131666.1"
                     /translation="MEERARFPEVAAKAGHYESFYVKACQPEGGKGVWIRHTVHKRPG
                     AEPNASIWFCLFDREAEGPRATKVTVPASRLDTPAGSWIRVADAEIGPGRAEGSVETE
                     ALSAAWSFTFAGNAEPCKYLPADWLYKAPLPKTKFLAPYPNARFQGRLVIDDEPFELS
                     GWPGMIGHNWGSEHAERWVWLEGTGFADAPDTYFDAGAARVRLGSRVSPWIPSGMLML
                     DGEAHRLGGLGAIRAATIEERPAVCTFLLPGKDIVVRGRVSAPEKDFVGWVYADPQGP
                     EHHTVNCSVADLELTIERRGFPPRQLTLPGGGAYELGMHATPGQP"
BASE COUNT          285 a          638 c          591 g          261 t
ORIGIN      
        1 gcgatgtgtc cgttcctatt acttcgaccg gtctaagtaa agggctctag ttggccattt
       61 tcccgctctg tccggtcatt tatgccctaa ggtgatcgct gccgtggagc cgactaatcg
      121 aaagggccgg gggatggagg agaccaaggc agaggagaag aaacgcaacg tgacgatctg
      181 caccgtgctc aagcacccgc tgcgggtccg catcctcgaa gtcctcaacg aaggcccgcg
      241 cagccccagc cagttcgtcg aggaagggct gatcccgaaa gagcactact ccacctacca
      301 gcaggccctc tcgctcgcct cctaccactt ccgcgagctg gagaaagaag gctgcctgga
      361 ggtggtcgaa tcgattccgc gccgcggcgc cgtcgagcac gtctaccgcg ggatgtcccc
      421 ccaggtcttc ttcagcgatg ccgaattcga agcgatgtcg ctggagacgc gccgccagct
      481 cacgcggatc tccctccagg ggctgatcgc gcgggccgac cgggctatca gcaccgagac
      541 cttcgacgcg cgctcggatc ggcacctgac ctgggtgccg atgcagctcg acgagcgggg
      601 ttggagcgaa gtgatcgccg ccctcgccgc gaccttcggc gaactgacgc agatccgcca
      661 cgacgccaaa gatcgcctcg ccgcctccgg ggaggaggtg gtgccggcca ccgtcgcgat
      721 gctcggcttc gaatcgcccc caccaccgcc gttgacgcgc agcgacgatt agctggtccc
      781 tcccccgggt cgccggggct agagttcacg catggaggaa cgggcgcggt ttcctgaggt
      841 ggcggcgaag gccggccact acgagagctt ctacgtcaag gcctgccaac cggagggcgg
      901 caaaggggtc tggatccgcc acaccgtgca caagcggccg ggggcggagc cgaacgcctc
      961 gatttggttc tgcctcttcg accgcgaggc cgagggaccg cgagcgacga aggtcacggt
     1021 gcccgcctcg cggctcgaca cccccgccgg ctcctggatc cgcgtcgccg acgccgagat
     1081 cgggccgggc cgcgccgagg ggtcggtcga gaccgaggcg ctcagcgccg cctggtcgtt
     1141 caccttcgcc ggcaacgccg agccctgcaa gtacctgccg gccgactggc tctacaaggc
     1201 gccgttgccg aagacgaagt tcctggcgcc ctacccgaac gcccgcttcc agggacggct
     1261 ggtgatcgac gacgaaccgt tcgagctctc gggctggcca gggatgatcg gccacaactg
     1321 gggcagcgag cacgccgagc gctgggtctg gctcgagggc accggcttcg ccgacgcgcc
     1381 cgacacctac ttcgacgccg gcgccgcccg cgtccgcctc ggctcccgcg tcagcccctg
     1441 gatcccctcg gggatgctga tgctcgacgg ggaagcgcac cggctcggcg gcctcggggc
     1501 gatccgcgcc gccaccatcg aggagcggcc cgcggtctgc acctttctcc tgcccggcaa
     1561 ggacatcgtc gtccgcggtc gcgtctcggc gccggagaag gacttcgtcg gctgggtcta
     1621 cgccgacccg cagggccccg agcaccacac cgtcaactgc tcggtcgccg acctcgagct
     1681 gacgatcgag cgccgcgggt tcccgccccg ccagctgacg ctgcccggcg gcggcgccta
     1741 cgagctgggg atgcacgcga ccccgggcca gccgg
//
LOCUS       DASWNU010000494         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_459723, whole genome shotgun sequence.
ACCESSION   DASWNU010000494 DASWNU010000000
VERSION     DASWNU010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459723"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VGB57_09695"
     CDS             complement(<1..310)
                     /locus_tag="VGB57_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131667.1"
                     /translation="MVRTTRVALLTTLSSLALAASPALAQTAATAEDLAAAQDQAAAE
                     ADAQPAPDSTIVITGTRRTDRTVADSPVPIDVIGAEAIANTGQPETNKILNDLVPSFN
                     F"
     gene            486..806
                     /locus_tag="VGB57_09700"
     CDS             486..806
                     /locus_tag="VGB57_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY0131668.1"
                     /translation="MSTPLTVDLPHKLGAQEARRRIERNVGKLTDHIPGGAQVSSRWT
                     GDRLDLDVGAMGQQVATQIEIQETVVRMTVMLPPALAFFKGMIEPLIRSQGAAMLEDK
                     SGKA"
     gene            complement(912..1271)
                     /locus_tag="VGB57_09705"
     CDS             complement(912..1271)
                     /locus_tag="VGB57_09705"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEY0131669.1"
                     /translation="MKILLPLLAAAVAAPAIAAPAPRPAEVSIPFANHRHAIRNFEAP
                     NDQIVYLEDRQRRWYRADLGHCVGLKWAHSIGYDTRGSINLDSFGAILVDGQRCPIVS
                     LTRSEGPPRKAKKAKGA"
     gene            1378..2679
                     /locus_tag="VGB57_09710"
     CDS             1378..2679
                     /locus_tag="VGB57_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0131670.1"
                     /translation="MRHVAIIGSGPAGYYTAEALQKLYGDEIRIDMIDRLPVPYGLIR
                     SGVAPDHQSIKAVYRRYEKVALSANVRFVGNVCVGPDVTVAELVGLYDAVVLATGAPN
                     DRPLDLPGSELPGVVGSAAFVGWYNGHPDFADLDPPLDVAAAAVIGNGNVALDVARIL
                     AKTRAEFAGSDIVGRALDALEGSTIRTIHVLGRRGPHQIAMTPKELGELGHLEAAAPR
                     VDLADFPPQADDALLEPGMRKSVTHLRDFANLAQDKARTIDFDFFAMPVAIEGEGRVE
                     RLIVERTELTDDLRSVGTGERYSIECGLVISCIGYQTPPIEGVPYEHGRGRFANRDGV
                     IGGGLYCVGWARRGPSGTIGTNRPDGFEVAETIHSACPPGDGGRTGGPGLDTLLHARG
                     VEPVAFRDWQRIEAAETGRAREGSPREKFTSIAEMIAALGR"
     gene            3036..3112
                     /locus_tag="VGB57_09715"
     tRNA            3036..3112
                     /locus_tag="VGB57_09715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3070..3072,aa:Pro,seq:cgg)
     gene            complement(3127..>4428)
                     /locus_tag="VGB57_09720"
     CDS             complement(3127..>4428)
                     /locus_tag="VGB57_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0131671.1"
                     /translation="VFSKRLEPEFAEEGRDPVAILDYIAATVDRPGFATTSPRFMAYI
                     PGGGLFHSALGDLLAAASNKYSGFASASPGAVRLENATTQWLARVVGYPDGAAGTLTS
                     GGSLANLVAIVAARDALDPDGGGAVYHSRFAHHCVDKALHIAGRGRAPRRPIGTDSGC
                     RMSPGALEEALRGDLAAGVRPWLVVASAGTVDTGAVDPLAEIAAICRRYGAWFHVDGA
                     YGGLFALCDEGRQKLSGIEQSDSVAVDPHKTLFLPYGTGAVLVREGGHLLRSFSASAD
                     YLQPLGESEVGPSPADMSPELTRHFRAMRLWLPLQIAGLGAFRAAQAEKLALARYLHA
                     RLAGLDGFDPGPPPDLSVVAFRYHPESGDCDQFNERLLQRVQSEGRVMLSGTRIDGRF
                     TLRCAILSFRTHIEHVDEVVEALLRAVAQLEPEPGEPRTGA"
BASE COUNT          697 a         1500 c         1569 g          662 t
ORIGIN      
        1 ggaagttgaa ggaggggacg agatcgttga ggatcttgtt ggtctcgggc tggccggtat
       61 tggcgatcgc ctcggccccg atcacgtcga tcggcaccgg gctgtcggcg acggtccggt
      121 cggtgcggcg ggtgccggtg atgacaatgg tcgaatccgg cgcgggctgg gcatccgcct
      181 cggcggcggc ctggtcctgg gcggcggcga gatcctccgc ggtggccgcg gtctgggcga
      241 gagcggggct cgcggcgagg gcgagcgagg aaagagtcgt cagaagtgcg acccgcgtcg
      301 tgcgaaccat ttgaattccc cccgaatgca gcgccgccgc cgccaagcct tcttagctta
      361 tgtcaatccg ggcggcagga aagggttttc cgctgcaatg caaaaatcac acggccactg
      421 tgcccgccgg accacagcgc gaaaagcctt ggaggagagc gccccgcgcc gctaagggcg
      481 cctgcatgag cacgcctttg accgtcgacc ttccccacaa gcttggagcc caggaggcgc
      541 ggcgccggat cgagcgcaac gtcggcaagc tgaccgacca tataccgggc ggagcccagg
      601 tgagctcgcg ctggaccggc gaccggctcg acctcgacgt cggagcgatg ggccagcagg
      661 tcgccaccca gatcgagatc caggagacgg tggtgcggat gacggtgatg cttccgccgg
      721 ccctcgcctt cttcaaggga atgatcgagc cgctgatccg aagccagggc gcggcgatgc
      781 tcgaggacaa gagcggcaag gcgtaggccg tcgagggcct ccaaccgtca ttgcgagcga
      841 agcgaggcaa tccagtgcgg cctccggggc ccgccggatt gcttcgtcgc ttcgctcctc
      901 gcaatgacga gctacgcgcc cttggccttc ttcgccttgc gcggcgggcc ttcgctccgg
      961 gtcagcgaaa cgatcgggca gcgctgcccg tcgaccagga tcgccccgaa gctgtcgagg
     1021 ttgatcgagc cgcgcgtgtc gtagccgatc gaatgggccc atttcaggcc gacgcaatgg
     1081 ccgaggtccg cccggtacca gcgcctctgc cggtcctcga gatagacgat ctggtcgttg
     1141 ggagcctcga agttgcggat cgcgtggcgg tggttggcga acgggatgct gacttcggca
     1201 ggcctggggg caggcgcggc gattgcgggg gccgctacgg cggcggcgag gagggggagg
     1261 agaatcttca tggcgctcta atgcaacggc cgggctgaac cggcgatgac gaaggcggct
     1321 tgcctcagcc cggcacggcg ctagcatcga gtccacattt gggagggctc gggcaagatg
     1381 cggcacgtgg cgatcatcgg ctccggcccg gcgggctatt acaccgccga ggcgctgcag
     1441 aagctctacg gcgacgaaat ccggatcgac atgatcgacc ggctgcccgt cccctacgga
     1501 ctgatccgct ccggggtcgc ccccgaccac cagtcgatca aggcggtcta ccggcgctac
     1561 gagaaggtgg cgctgtcggc caacgtccgc ttcgtcggca acgtctgcgt cggtcccgac
     1621 gtgacggtgg ccgagctcgt cgggctttac gacgcggtcg tcctcgccac cggggcgccg
     1681 aacgaccggc cgctcgatct tcccgggagc gagcttcccg gcgtggtcgg ttcggccgcc
     1741 ttcgtcggct ggtataacgg ccatcccgat ttcgccgatc tcgacccgcc gcttgacgtc
     1801 gccgccgccg ccgtgatcgg caacggcaac gtcgccctcg acgtcgcccg gatcctggcc
     1861 aagacccggg cggagttcgc cggctccgac atcgtcggcc gcgccctcga cgccctggag
     1921 ggaagcacga tccggacgat ccacgtgctc ggccggcgtg ggccccacca gatcgccatg
     1981 acccccaagg agctgggcga actgggccat ctcgaggccg ccgccccccg ggtcgacctc
     2041 gccgacttcc cgccgcaggc cgacgacgct ttgctcgagc cgggcatgcg caagtcggtc
     2101 acccatttgc gcgacttcgc caacctggcg caggacaagg ccaggacgat cgacttcgac
     2161 ttcttcgcaa tgccggtggc gatcgagggc gagggccggg tcgagcgcct gatcgtcgag
     2221 cgcaccgagc tcaccgacga cctgcgctcg gtcggcaccg gcgagcgcta ttcgatcgaa
     2281 tgcggcctgg tgatcagctg catcggctac cagaccccgc cgatcgaggg cgtcccctac
     2341 gagcatggcc ggggccgctt cgccaatcgg gacggggtga tcggaggcgg cctctattgc
     2401 gtcggctggg cccggcgcgg gccttcgggc acgatcggca ccaaccggcc cgacggcttc
     2461 gaggtcgccg agacgatcca ctcggcctgc ccgcccggcg acggcggcag gacgggcggg
     2521 cccggcctcg acaccctgct ccacgcccgg ggagtcgagc cggtcgcctt ccgcgactgg
     2581 cagcgcatcg aggcggccga gaccggccgc gcccgcgagg gcagcccccg cgagaagttc
     2641 accagcatcg ccgagatgat cgccgcgctg gggcggtagc cttccgcggc tgcggcatcc
     2701 ccgtggattt atctcacaat gtcaaagagc ccctgccgat ccgcgatccg gccagacgaa
     2761 caatatagga acatctagtc catatatctc ctacagataa gtccgttccg gaagttgcag
     2821 cccgccccgg gtcccggctg ccgcgtaacc ccggactcga tccggggtcc atggacgccg
     2881 ccgaccgagc caggctcgcc gccgtgttca tggatggccg gatcgggccc ggcatgacga
     2941 aggatggccg gccgggacga ggcgcttgcg ctcgccccaa ccctccctta ttgagcgcgg
     3001 cctgtctccc gggcggcctt agagccggat ccggtcgggg agtagctcag cctggtagag
     3061 cactgtcttc gggaggcagg ggccggaggt tcgaatcctc tctccccgac caatttttcc
     3121 ttacggttag gcgccggtcc gcggttcgcc gggttccggc tcgagctggg ctacggcgcg
     3181 cagcagcgcc tcgacgacct cgtcgacatg ctcgatatgg gttcggaagc tgaggatggc
     3241 gcagcgcagg gtgaagcggc cgtcgatccg ggtgccgctg agcatcaccc ggccttccga
     3301 ctgcacgcgc tggagcaggc gctcgttgaa ctggtcgcaa tcgccgctct ccgggtggta
     3361 gcggaaggcg acgacggaga gatcgggcgg tggccccgga tcgaagccgt cgagcccggc
     3421 gagccgggcg tggaggtagc gcgccagagc gagcttttcg gcctgggccg cccggaaggc
     3481 gccgaggccg gcgatctgaa gcggcagcca caggcgcatc gcccggaaat gccgggtaag
     3541 ctcgggcgac atatccgccg gcgaaggccc gacctccgat tcgccgagcg gctggagata
     3601 gtcggcgctg gcgctgaacg agcgcagcag gtgtccgccc tcgcggacca ggacggctcc
     3661 ggttccgtag ggcaggaaca gggtcttgtg cggatcgacg gcgacgctgt ccgactgctc
     3721 gatcccgctc agcttttgcc gcccttcgtc gcaaagggcg aacagcccgc cataagctcc
     3781 gtcgacatga aaccaggcgc cgtagcggcg gcagatcgcg gcgatctcgg cgagaggatc
     3841 gacggctccc gtgtccaccg ttccggccga ggcgacgacg agccagggcc ggaccccggc
     3901 ggcgaggtcg ccccggagcg cctcctccag cgccccgggc gacatccggc agccggagtc
     3961 ggtgccgatc ggccgcctcg gcgccctccc ccgcccggcg atgtgaagcg ccttgtcgac
     4021 gcaatgatgg gcgaagcggc tgtggtagac cgctccgccg ccgtcgggat cgagcgcgtc
     4081 gcgggcggcg acgatggcca ccagattggc gaggcttccg ccggaggtga gggtgccggc
     4141 ggcgccgtcc ggatagccga cgaccctcgc cagccactgg gtggtggcat tttccagtcg
     4201 gaccgcgccc gggctggccg aggcgaatcc ggaatatttg ttggacgcgg cggcaagcag
     4261 atccccgagc gccgaatgga agaggccgcc gcccgggatg taggccatga agcgcggcga
     4321 ggtggtcgcg aagcccggcc ggtcgactgt cgcggcgata tagtcgagaa ttgccaccgg
     4381 atcccgcccc tcctccgcga attcgggctc cagccgcttc gagaagac
//
LOCUS       DASWNU010000495         5514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_45203, whole genome shotgun sequence.
ACCESSION   DASWNU010000495 DASWNU010000000
VERSION     DASWNU010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45203"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..874
                     /locus_tag="VGB57_09725"
     CDS             197..874
                     /locus_tag="VGB57_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY0131672.1"
                     /translation="MLPSPLTSIDCWIFDLDNSLYPASANLFELIDIRMGEYIAKRLG
                     CTMAEARIIQKGFFRDHGTTLAGLMKEHGVEPRDFLDYVHDIDFARLTADPRVVAALD
                     RLPGRKFVFTNGDESYARRVLDRLGLANAFDGVHDIHAMDYVPKPDPRAYAAMCQRHR
                     IEPTSALFAEDMARNLVPAKQLGMTTVWVDNGSEQASHDADPAFIDYRITDIGDWLSE
                     ITGGEPA"
     gene            871..1692
                     /gene="dapD"
                     /locus_tag="VGB57_09730"
     CDS             871..1692
                     /gene="dapD"
                     /locus_tag="VGB57_09730"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0131673.1"
                     /translation="MTEALRTVIEDAWERRESLGGEEAEVKQAVEAVIALLDSGEARV
                     AEKGEGGWTVNQWLKKAVLLSFRLTPMEAIGGGPGGSHWWDKVPSKFAEWGPDRFAGA
                     GFRAVPGAIVRRGAFIGKGAVLMPSFVNIGARVGEGTMVDTWATVGSCAQIGRNVHIS
                     GGTGIGGVLEPLQAGPVIIEDDCFIGARSEVAEGVIVEQGAVLSMGVFLGASTKIVDR
                     ATGEIFMGRVPSYSVVVPGTLPGKDGGPGLACAVIVKRVDARTRSKTSINELLRD"
     gene            complement(1720..2334)
                     /locus_tag="VGB57_09735"
     CDS             complement(1720..2334)
                     /locus_tag="VGB57_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0131674.1"
                     /translation="MRPILPLLLLLAACGDGGEPANATGPDQPGWSGPVEAQPTGRLD
                     RSNAGKTAPATPFEDPEGAPTSLADFRGRPVLLNLWATWCAPCIAEMPTLDRLAAREA
                     DRLHVLTVSQDLDGREKVEAFFARQGYRNLDTWLDPQMALMTGLGADTLPTTILYDAR
                     GREVWRVVGMEDWESGRAALLLREAATPAKAGASSGESLGAGSP"
     gene            2426..2677
                     /locus_tag="VGB57_09740"
     CDS             2426..2677
                     /locus_tag="VGB57_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131675.1"
                     /translation="MRILLLLPAFALLAACGNLEPLQPKAGAKDGGGMPAKPAMAARA
                     PTTDELLTPPPQARPARQDDGLRRSEEREDDPFDLPPTG"
     gene            2712..3569
                     /gene="galU"
                     /locus_tag="VGB57_09745"
     CDS             2712..3569
                     /gene="galU"
                     /locus_tag="VGB57_09745"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY0131676.1"
                     /translation="MKKVRKAVFPVAGLGTRFLPATKAVPKEMLTVVDRPLIQYAVEE
                     ALEAGIEQMIFVTGRGKTALEDHFDIAYELEATMRGRGKSLDVLNSTRMPPGSVVAVR
                     QQEPLGLGHAVWCAREIVGDEPFAVFLPDEVMVGRPNCTRQLVDAYEKVGGNVVAVLE
                     VPPEETHKYGVVDPGARDGKLTEIKGLVEKPAPGTAPSNLMLPGRYVLQPEIMEALGK
                     VGRGAGGEIQLTDALAAMIGKQPFHGLTFDGERHDCGHTLGFVIANLALALKRDDVAP
                     ALRAYLAGL"
     gene            3570..4526
                     /locus_tag="VGB57_09750"
     CDS             3570..4526
                     /locus_tag="VGB57_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY0131677.1"
                     /translation="MSCAAILVGDGAVRTVTSEEAALYAGPGFIWVHIDAGTDEELGL
                     LRGNDIPDVAANALVATETRPRCDRIEEGAIVNLRGPAAEELDDSDRLVSIRMWVRRG
                     KVNSVTRRPLAATAAVAAQMEAGRILDPGDLVAAFARAISKQLDPEVAQLGDTLDDYE
                     SELDDSRALYRLRTAITRIRSDAIAYRRFVAPNRDALVTLAELDFLWLAEEDRLHIRE
                     AADRFARMTEELEAVRERAALLHEQLTDLRGEQMDSRSLVISIVAFIFLPLTFITGLL
                     GMNVEGIPYANEPWAFWGVVALCILIGVLVLAWFMWRHWLRR"
     gene            complement(4618..5040)
                     /gene="ribH"
                     /locus_tag="VGB57_09755"
     CDS             complement(4618..5040)
                     /gene="ribH"
                     /locus_tag="VGB57_09755"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0131678.1"
                     /translation="MARLLIVEARFYEHLNDLLLAGARAAIEAAGHEHETVTVPGALE
                     VPAAIALGAETERYDGFVALGVVIRGETYHFEIVAGESARGIMALTLDGLAIGNGILT
                     VENEAQALARARPDEKDKGGEAAKAALAMMKLRERFSS"
     gene            complement(5043..>5514)
                     /locus_tag="VGB57_09760"
     CDS             complement(5043..>5514)
                     /locus_tag="VGB57_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0131679.1"
                     /translation="EASFESRWGGTWTARSFLNKALGTEQIALVKGKIDPSKPTLVRM
                     HQLSLFADAFGEEGGRGGMLRGAMKIIAEEGSGVIVVLNRAMPDALSAALNLRSGKAA
                     KGPDELRDYGGGAQILSALGVHDMVLLTNTHHTPVALGGYGLAIVGERPIPREE"
BASE COUNT          842 a         1975 c         1815 g          882 t
ORIGIN      
        1 ccgccgcctc cgccgccgca gcccgaaagg acgagcgcgg ccgcgcaggc ggtcaggagc
       61 agggcattgc gagtgagcct ggcgttcatc cccggcctcc agctaactgg ttcaaatttg
      121 ggagttttcc agttcctatc ctattccgcc gatgctttca atttgctgaa gaccggatgc
      181 gccctatgag gcgcccatgc tgccctcccc gctcacatcg atcgactgct ggatcttcga
      241 cctcgacaac agcctctatc cggcctcggc caatttgttc gagctgatcg atatccgcat
      301 gggcgagtat atcgccaaaa ggctcggctg caccatggcc gaggcgcgaa tcatccagaa
      361 aggtttcttt cgggaccacg gcaccacctt ggccgggttg atgaaggagc atggagtcga
      421 gccgcgcgac ttcctcgatt acgttcatga catcgacttc gcccggctca ccgcagaccc
      481 gagggtggtg gcggcgctcg accggctgcc gggccgaaag ttcgtcttca ccaacggcga
      541 cgaatcctat gcccggcggg tgctcgaccg gctcggcctc gccaacgcct tcgacggagt
      601 ccatgacatc cacgccatgg actatgtccc caagcccgat ccgcgcgctt atgcggcgat
      661 gtgccagcgc caccggatcg agccgacgag cgccctcttc gccgaggaca tggcgaggaa
      721 cctggttccg gccaagcagc tcggaatgac caccgtctgg gtcgacaacg gctcggagca
      781 ggccagccac gacgccgatc ccgcctttat cgactatcgc attaccgata tcggcgactg
      841 gctgagcgag atcacgggag gagagccggc atgaccgagg cgttgaggac cgtgatcgag
      901 gacgcctggg agcggcgcga atcgctgggc ggcgaggaag ccgaagtgaa gcaggccgta
      961 gaggccgtga tcgcattgct cgattcggga gaggctcggg tcgccgagaa gggcgagggc
     1021 ggctggaccg tcaaccaatg gctcaagaaa gccgttctcc tctccttccg gctgaccccg
     1081 atggaggcga tcggaggcgg tccgggcggc tcccattggt gggacaaggt gccctccaag
     1141 ttcgccgaat gggggcccga ccgcttcgcc ggcgccggct tccgcgccgt ccccggcgcc
     1201 atcgtccggc gcggcgcctt catcggcaag ggcgcggtcc tgatgccgag cttcgtcaac
     1261 atcggcgccc gggtcggcga aggcacgatg gtcgacacct gggcgacggt cgggagctgc
     1321 gcccagatcg gccgcaacgt ccacatctcc ggcggcaccg gtatcggcgg agtgctcgag
     1381 ccgctccagg ccgggcccgt catcatcgag gacgattgct tcatcggcgc ccgctccgag
     1441 gtcgccgagg gcgtgatcgt cgagcaaggc gcggttctct cgatgggcgt cttcctcggc
     1501 gcctcgacca agatcgtcga ccgggcgacc ggcgaaatct tcatgggccg cgtcccgagc
     1561 tattcggtgg tcgtccccgg cacgctgccc ggcaaggacg gcggtccagg cctcgcctgc
     1621 gccgtcatcg tcaagcgggt cgacgcccgc acccgctcca agaccagcat caacgagctg
     1681 cttagggatt agcccgtcat cccggcgcaa cccgggacct cagggagatc cagcgccgag
     1741 gctttcaccc gacgaggccc cggctttcgc cggggtggcg gcttccctga gcagcagcgc
     1801 cgccctgccg ctctcccaat cctccattcc gactacccgc cagacctcgc gcccccgggc
     1861 gtcgtagagg atcgtcgtcg gcagtgtgtc ggccccgagg ccggtcatca gcgccatttg
     1921 cgggtcgagc caagtatcga ggttacgata gccctgcctg gcgaagaagg cctcgacctt
     1981 ctcccggccg tccaggtcct ggctgacggt cagcacgtgg agccggtccg cctcccgggc
     2041 cgcaagccgg tcgagcgtcg gcatttcagc gatgcagggg gcgcaccagg tcgcccagag
     2101 attgagaagc accggtctgc cgcggaagtc ggcgaggctc gtcggcgcgc cttccggatc
     2161 ctcgaacggc gtcgcgggcg ccgttttccc ggcattcgag cgatcgagcc gccccgtcgg
     2221 ctgcgcttcc accggcccgc tccagcccgg ctggtccggg ccggtggcgt tggccggttc
     2281 cccgccgtca ccgcaggcgg ccaggagcag gagcaggggc aggatcggcc gcatcgccgc
     2341 cgactaggtt gccgccgtcc gcgcggcaag ggcgcggatt cgccttggcc gtccccgccc
     2401 gcctgcgcta tggcggcgcc tcaccatgcg catcctgctc ctcctccccg ccttcgcctt
     2461 gctcgccgcc tgcggcaatt tggagccgct ccagcccaag gccggcgcca aggacggggg
     2521 gggcatgccg gcgaagcccg caatggcggc ccgggccccg accaccgacg agcttctgac
     2581 cccgccgccc caggcccgtc ccgcccgcca ggacgacggg cttcgccgct ccgaagagcg
     2641 cgaggacgat cccttcgacc ttcccccgac cggctgacgc gccggcgccc gcacaatctt
     2701 cggagaccaa gttgaagaaa gtcagaaagg cggtcttccc cgtcgccggc ctcggtacgc
     2761 gattcctgcc cgccaccaag gccgtgccca aggagatgct gaccgtcgtc gaccggccgc
     2821 tcatccaata tgcggtggag gaggcgctgg aggcggggat cgagcagatg atcttcgtca
     2881 ccggccgcgg caagaccgcg ctcgaggacc atttcgacat cgcctacgag ctcgaggcga
     2941 ccatgcgcgg ccgcggcaaa tcgctcgacg tcctgaacag cacccggatg ccgcccgggt
     3001 cggtggtcgc ggtgcgccag caggagccgc tcggactcgg ccatgccgtc tggtgcgccc
     3061 gcgagatcgt cggcgacgag ccgttcgcgg tgttcctgcc cgacgaggtg atggtcggtc
     3121 gcccgaactg cacccgccag ttggtcgacg cttacgagaa ggtcggaggc aacgtcgtcg
     3181 ccgtgctcga ggtgccgccc gaggagaccc acaaatacgg agtcgtcgat cccggcgccc
     3241 gcgacggcaa gctcaccgag atcaaggggc tggtcgagaa gccggcgccc gggacggcgc
     3301 cttcgaacct gatgcttccc ggccgctacg tcctccagcc cgagatcatg gaggcgctcg
     3361 gcaaggtcgg gcgcggcgcc ggcggcgaga tccagctcac cgacgcgctt gcggcgatga
     3421 tcggcaagca gcccttccac ggcctcacct tcgacggcga acggcacgat tgcggccaca
     3481 cattgggctt cgtcatcgcc aacctcgccc tggcgctgaa gcgggacgac gtcgctccgg
     3541 cgctgcgcgc ctatctggcc ggcctttagg tgagctgcgc ggcgatcctg gtcggcgatg
     3601 gagccgtccg gacggtcacc tccgaggaag ccgccctcta tgccggcccc ggcttcatct
     3661 gggtccatat cgacgccggc accgacgagg agctgggcct gcttcgcggc aacgacattc
     3721 cggacgtcgc cgccaacgcc ctggtcgcga ccgagacccg cccgcgctgc gaccggatcg
     3781 aggaaggggc gatcgtgaac cttcgggggc cggccgccga ggagctggac gattccgacc
     3841 ggctggtctc gatccggatg tgggtgaggc ggggcaaggt caacagcgtc acccgccggc
     3901 ctctggccgc caccgcggcc gtggcggcgc agatggaggc ggggagaatc ctcgatccgg
     3961 gcgacctcgt cgccgccttc gctcgcgcca tttcgaagca gctcgatccc gaagtcgccc
     4021 agctcggcga cacgctcgac gattacgaga gcgagctcga cgacagccgc gccctctacc
     4081 ggctgcgcac cgcgatcacc cggatccgct ccgacgccat cgcctatcgc cgcttcgtcg
     4141 cgcccaatcg cgacgccctg gtcaccctcg ccgagctcga cttcctgtgg ctggccgaag
     4201 aggaccggct ccacatccgc gaggcggcgg atcgcttcgc ccggatgacc gaggaactgg
     4261 aagcggtgcg cgagcgcgcc gccctcctcc acgagcagct caccgacctg cgcggcgagc
     4321 agatggattc gcgctcgctg gtcatctcga tcgtcgcttt catcttcctg ccgctgacct
     4381 tcatcaccgg cctgctcgga atgaacgtcg agggcatccc ttatgcgaat gagccctggg
     4441 ccttctgggg cgtcgtcgcc ttgtgcatcc tgatcggcgt gctggtgctc gcctggttca
     4501 tgtggcggca ctggcttcgg cgctaaaata ttcccgtcat cccggcgaaa gccgggacct
     4561 caggccgaga agtctcggcg ggtctccccg aggtcccggc tttcgccggg atgacggcta
     4621 gctcgaaaac cgctccctca gcttcatcat cgccagcgcc gccttcgccg cctcgccgcc
     4681 tttgtccttc tcgtcgggcc gggcgcgggc cagggcctgg gcctcgttct cgaccgtcaa
     4741 tatgccgttg ccgatcgcca gcccatcgag ggtcagcgcc atgatcccgc gcgcgctttc
     4801 gccggcgacg atctcgaaat gataggtctc gccgcggatc accacgccca gggcgacgaa
     4861 gccgtcatag cgctccgtct cggcccccag agcgatcgcg gccggcactt cgagcgcccc
     4921 gggcacggtg acggtctcat gctcgtgccc ggccgcctcg atcgccgcgc gcgcgcctgc
     4981 caggagcagg tcgttgagat gttcgtagaa gcgcgcctcg acgatcagca accgggccat
     5041 gcctattcct cccgcgggat cggccgctcg ccgacgatgg cgaggccgta gccgccgagc
     5101 gcgaccggcg tgtggtgggt gttggtcagg agcaccatgt cgtggacccc gagcgccgag
     5161 aggatctggg cgccgccgcc atagtcgcga agctcgtccg ggcccttcgc cgccttgccg
     5221 gagcgcaggt tgagcgcggc ggacagggcg tccggcatcg cgcggttgag gacgacgatg
     5281 actccgcttc cttcctcggc gatgatcttc atcgcccccc gaagcatccc gccccgaccg
     5341 ccttcttcgc cgaacgcgtc ggcgaagagg ctgagctggt gcatcctgac cagggtcggc
     5401 ttcgacggat cgatcttccc tttgacgagg gcgatctgct cggtgccgag cgccttgttg
     5461 aggaagctcc tggcggtcca ggtgccgccc cagcggctct cgaagctcgc ctcc
//
LOCUS       DASWNU010000496         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_97243, whole genome shotgun sequence.
ACCESSION   DASWNU010000496 DASWNU010000000
VERSION     DASWNU010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97243"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGB57_09765"
     CDS             <1..255
                     /locus_tag="VGB57_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131680.1"
                     /translation="WCELKTGVDRVNLGLSQVEEAGGKGGATLTFGVEDIEAAKAELD
                     SLGIRQDGAILEIPGMVRLLTFYDPDHNALMLYEDLQGAG"
     gene            513..1475
                     /gene="mdh"
                     /locus_tag="VGB57_09770"
     CDS             513..1475
                     /gene="mdh"
                     /locus_tag="VGB57_09770"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY0131681.1"
                     /translation="MARTKIALIGAGMIGGTLAHLAAIREMGDIVLVDVAEGIPQGKA
                     LDLAQAGPVEGFDSKLTGTQDYADIKGADVCIVTAGVARKPGMSRDDLLGINLKVMKA
                     VGEGIKAHAPDSFVICVTNPLDAMVWALREFSGLPHQRVVGMAGVLDSARFRHFLAEE
                     FKVSVEDVTAFVLGGHGDTMVPLPANSAVAGIPVPDLIRMGWSTQERIDSIVQRTRSG
                     GGEIVGLLKTGSAYYAPATAALAMAESFLKDKKRLLPAAAYLSGQYGVDDLYVGVPVV
                     IGAGGVERIVEIELDESAKANFQVSVEAVKELLEACRGIDESLR"
     gene            1484..2020
                     /locus_tag="VGB57_09775"
     CDS             1484..2020
                     /locus_tag="VGB57_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131682.1"
                     /translation="MGWPLLLLLAPATAEPPKASASAELAEIWISACLDGQLRLSPDR
                     IREVGAGGVEAYWQPERRQRNAHYYKILKPTGAGLIITDYDPPTHQGFKSTCELVSRG
                     KDLKALWPRVASALGGKSVQHIRDMDIYTVDNPAEGYRIEVRSWFLSIGHYTDKKVWE
                     ARARSKKPTAQAEDSIFE"
     gene            2017..2601
                     /locus_tag="VGB57_09780"
     CDS             2017..2601
                     /locus_tag="VGB57_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131683.1"
                     /translation="MKPALPSLAIPAALFLIAAPAAAAEREMLDAFKSACARTGDVGA
                     MKADAAASGWKEMAEETEPRIARLVKLGRDAVEADSKVSGATFRRALGGRDVFLIVSR
                     YEDKSGYWGVGCRLYDFDAAAPLKAATLEAWMGKPPTGVQEPAPGLTKRLWEPSGWRY
                     GITVEVAHVPQNHPLGKTYGLSGNILTAQAIGGF"
     gene            2602..>2725
                     /locus_tag="VGB57_09785"
     CDS             2602..>2725
                     /locus_tag="VGB57_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0131684.1"
                     /translation="MSILVNSSTKVITQGMTGETGTFHTEQALAYGTRMVAGVTP"
BASE COUNT          468 a          895 c          904 g          458 t
ORIGIN      
        1 tggtgcgagc tcaagaccgg cgtcgaccgc gtcaatctgg gactctcgca ggtcgaggag
       61 gccggcggca agggcggcgc gaccctgacc ttcggagtcg aggacatcga ggcggcgaag
      121 gcggagctcg attcgctggg aatccggcag gacggagcga tcctggaaat accggggatg
      181 gtccggctgc tcaccttcta cgatcccgat cacaatgcgc tgatgctcta cgaggatctg
      241 cagggggcgg ggtgatgtaa ttcctccccg cgttttcgcg gggaggggga ccgcgcgaag
      301 cggggtggag gggttcttca gagtcagcag gacctctcca ccatgcttcg catggtcccc
      361 ctccccggca aatgccgggg aggattttcc tttctttccc tcccccaccc ggttgctccc
      421 tcccgccttc gggtctaagc ggacgccaca tttggcggcg tcctgccgcc ggttccgctt
      481 cgactgacga aagaaaggct ggagcagccc ttatggctcg aacgaaaatc gcgctcatcg
      541 gcgccggcat gatcggcggc acactggccc acctcgcggc gatccgcgaa atgggcgata
      601 tcgtcctcgt cgacgtcgcg gagggcattc cgcagggcaa ggcgctcgac ctcgcccagg
      661 ccggcccggt cgaaggcttc gactcgaagc tcaccggcac ccaggattat gccgacatca
      721 agggcgccga cgtctgcatc gtcaccgccg gggtcgcccg caagccgggc atgagccgcg
      781 acgatctgct cgggatcaat ctcaaggtga tgaaggcggt cggcgagggc atcaaggcgc
      841 acgcgcccga cagcttcgtc atctgcgtca ccaacccgct cgacgcgatg gtctgggcgc
      901 ttcgggagtt ttccggactt ccgcaccagc gcgtcgtcgg aatggcgggc gtgctggatt
      961 cggcccgctt ccgccatttc ctcgccgagg agttcaaggt ctcggtcgag gacgtcaccg
     1021 ccttcgtgct cggcggccat ggcgacacga tggtgccgct gccggctaat tccgcggtgg
     1081 cgggaatccc cgttcccgac ctcatcagga tgggctggtc gacccaggag cggatcgact
     1141 ccatcgtcca gcgcacccgc tcgggcggcg gcgagatcgt cggattgctc aagaccggct
     1201 cggcttatta cgccccggcg acggcggcgc tggcgatggc cgagagcttt ctcaaggaca
     1261 agaagcgcct cctccccgcc gccgcctacc tctcaggcca gtacggcgtc gacgacctct
     1321 acgtcggagt gccggtggtg atcggcgccg ggggggtcga gcggatcgtc gagatcgagc
     1381 tggacgaaag cgccaaggcg aacttccagg tttcggtcga ggcggtgaag gaactgctcg
     1441 aggcctgccg cggcatcgac gaaagcctcc gctaggcccg tcgatggggt ggccgctact
     1501 cctccttctt gcgccggcga cggcggagcc tccaaaggca tccgcttcgg cggagcttgc
     1561 ggagatctgg atctcggcat gcctcgacgg ccagctgagg ctctccccgg accgaatccg
     1621 ggaggtgggt gctggcgggg tcgaggccta ttggcagccg gagcgccgcc agcgtaacgc
     1681 ccactattac aagattctaa agccgacggg agcggggctg atcatcaccg actacgatcc
     1741 ccccacccac cagggattca agagcacttg cgaactggta agtcgcggta aagacctcaa
     1801 ggcgctgtgg ccccgcgtcg cgagcgcgct cggcggcaag tccgtccagc acatccgcga
     1861 catggacatc tacacggtgg ataatccggc cgaggggtac aggattgaag ttcgctcctg
     1921 gttcctgagc atcgggcatt atacggacaa gaaagtatgg gaggcccgcg cgcgatcgaa
     1981 gaagccaacg gcccaggcag aggattctat cttcgaatga agcctgccct gccttccctc
     2041 gccatcccgg cggccctgtt ccttatcgct gcgccggcgg ctgccgccga gcgcgagatg
     2101 ctcgacgcct tcaagtcggc ctgcgcccgc accggcgatg tgggggcaat gaaggccgac
     2161 gcggccgcct cgggctggaa ggaaatggcc gaggaaacgg agcctcggat cgcccgtctg
     2221 gtcaagctcg gccgcgacgc cgtcgaagcc gattccaagg tgagcggtgc caccttccgc
     2281 cgcgccctgg gcgggcgcga cgtcttcctc atcgtctcgc gttacgagga taagtccggt
     2341 tattgggggg tcggctgtcg gctctacgat ttcgacgccg ccgcgccctt gaaggctgcg
     2401 actttggagg cctggatggg caagccgccg actggcgtcc aggagccggc cccgggcctt
     2461 acgaagcgac tgtgggagcc gtccggctgg cgctacggga tcaccgtcga ggttgcccac
     2521 gttccccaga accacccgct cggaaaaact tacggcctca gcggcaacat cctcaccgcc
     2581 caggccattg gaggcttttg aatgtcgatc ctcgtcaaca gctcgaccaa ggtcatcacc
     2641 caggggatga ccggcgagac cggcaccttc cacaccgagc aggcgctcgc ctacgggacc
     2701 aggatggtcg ccggagtgac tccgg
//
LOCUS       DASWNU010000497         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_149943, whole genome shotgun sequence.
ACCESSION   DASWNU010000497 DASWNU010000000
VERSION     DASWNU010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149943"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1040
                     /gene="epsC"
                     /locus_tag="VGB57_09790"
     CDS             345..1040
                     /gene="epsC"
                     /locus_tag="VGB57_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEY0131685.1"
                     /translation="MLPGLFAYLDSVKARDPAPRTRWEVLLYPGVWALGFHRLAHWLY
                     KGELYFLARLVNHISRFLTAIFIHPCASVGRNFFIDHGFVVIGETATIGNDVTIYQQV
                     TLGGTNPTDGVAGKRHPTISDQVIIGSGAQVLGPIVVGPRARIGANAVVTKDVPEGAT
                     MIGIPARSTLLSAEQSSSGNFLPYGTPCSEQFDPATQKLEILQCEVESLRKRLAELIE
                     ERDGQSRKGRGRA"
     gene            1040..1378
                     /locus_tag="VGB57_09795"
     CDS             1040..1378
                     /locus_tag="VGB57_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEY0131686.1"
                     /translation="MGNVLPFPGASSPQIGFTRAELTRIIDLYGRMVAAGLWRDYAVE
                     FGRDGAAFWAFRRTAERPEYRIEKKPGLRNRQGMWALVNEAGMVVRRGDELGPVLAPL
                     ERRLMKAVNG"
     gene            complement(1516..>2410)
                     /locus_tag="VGB57_09800"
     CDS             complement(1516..>2410)
                     /locus_tag="VGB57_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131687.1"
                     /translation="AVGWIGHAIGPNIGGDGPSPFAGLFVAATAWGLAEFFTRKRRMA
                     LPSILLLLAFTGGVLLTAFVTLVLAIGELAFEDNEQLFALVIAASAAVAAGATWLHWR
                     RFRVPITVAVGAATLVGVVLALIAYAVGDVPQLENALLASALVLGLGVFLFAMWWDSS
                     DRARETRRSDVAFWLHLLSAPLIVHPIFSLLGLTDGDATIGEAMVVLGVYVLLGLTAL
                     AIDRRALLVSALAYVLFALISLFREFGAVELNIALAAFVIGSGLLLLSAFWHSVRRSI
                     VRPLPSGLQARLPVTDRAVAA"
BASE COUNT          421 a          802 c          833 g          354 t
ORIGIN      
        1 ccttcgtcac gatgctcgga gtcgagcggg cccggcggca gtcgaagctc ctcgtcgacc
       61 aagcggtcga gcatctgcag gatttcgggc gcgattcgga cctgctcgcg gcgatcgcga
      121 ggttcgcggt gagccgggat cattagcctc cagtcctccc cgagaaatct cggggagggg
      181 accgggcgac gcctggtgga ggggttcttc ggagttagaa aggcccctcc accatgcttc
      241 gcatggtccc cctcccctgc aaatacaggg gaggatttta gactctctgg cattttgccc
      301 caacctctct taccttgccc gccaaggagc agtggggagt agttatgctg ccggggcttt
      361 tcgcctatct cgactcagtg aaggcccgcg atccggcgcc ccggacccgc tgggaagtgc
      421 ttctctatcc cggcgtctgg gcgctgggtt tccatcgcct cgcccactgg ctgtacaagg
      481 gcgagctcta tttcctcgcc cgcctcgtca accatatcag ccgcttcctc accgcgatct
      541 tcatccatcc ctgcgcctcg gtgggccgga atttcttcat cgaccacggc ttcgtcgtga
      601 tcggcgagac tgcgacgatc ggcaacgacg tcaccatcta ccagcaggtc accttgggcg
      661 gcaccaaccc gaccgacggg gtcgccggca agcgccaccc gacgatcagc gaccaggtca
      721 tcatcggctc gggcgcccag gtgctcgggc cgatcgtcgt cggcccgcgc gcccggatcg
      781 gcgccaatgc ggtggtcacc aaggacgtgc ccgaaggcgc gaccatgatc ggcattccgg
      841 cgcgctccac cctgctcagc gccgagcaga gcagctccgg caatttcctt ccctacggga
      901 cgccctgctc cgagcagttc gatccggcca cccagaagct cgaaatcctc cagtgcgagg
      961 tcgagagcct gaggaagcgg ctcgccgagc tgatcgagga gcgggacggc cagtccagga
     1021 aggggcgcgg ccgcgcctga tgggcaacgt cctccctttt ccgggagcct cttcgccgca
     1081 gatcggcttc acccgggccg agctgacgcg gatcatcgac ctgtacggtc ggatggtggc
     1141 ggcggggctg tggcgcgatt acgcggtcga gttcggtcgc gacggagccg ccttctgggc
     1201 cttccggcga accgccgagc ggcccgaata caggatcgag aagaagcccg gccttcgcaa
     1261 ccgccagggc atgtgggcgc tggtcaacga ggccgggatg gtggttcgcc gcggcgacga
     1321 gctcggcccg gtgcttgcgc cgctcgagcg gcggctgatg aaagcggtca acgggtaata
     1381 tttgcctccc catcgaagat ggggaggggg accccgcgta gcggggtgga ggggtaatgg
     1441 cgcaggtctg tgccagctac ccctccatcg ccttcggcgg tccccctccc catgctgcgc
     1501 atggggaggc agaagtcagg ccgctaccgc cctgtctgtc accggcagcc ttgcctggag
     1561 acccgacggc agcgggcgga cgatcgagcg gcggactgag tgccagaagg cggagagaag
     1621 cagcagcccg gagccgatca cgaaggcggc gagggcgatg ttcagctcca ccgctccgaa
     1681 ctcgcggaac aggctgatca gagcgaacag cacgtaggcg agcgccgaga ccagcagcgc
     1741 ccgccggtcg atcgcaagcg cggtcaggcc gagcaggaca tagactccga gcaccaccat
     1801 ggcctcgccg atcgtcgcat cgccgtcggt gaggccgagc agcgagaaga tcgggtggac
     1861 gatcagcggc gccgacagca gatggagcca gaaggcaacg tccgagcgcc gggtctcgcg
     1921 ggcgcggtcg gaggaatccc accacatggc gaagaggaag accccgaggc cgaggacgag
     1981 ggcgctcgcc agcaaggcgt tctcgagctg cggcacgtcg ccgaccgcat aggcgatcag
     2041 cgcgagcacg actccgacga gcgtcgccgc gccgaccgcg accgtgatcg ggacccggaa
     2101 ccggcgccag tgaagccagg tcgcgcccgc ggcgaccgcg gccgaagcgg cgatgaccag
     2161 cgcgaacagc tgttcgttgt cctcgaaggc cagctcgccg attgcgagga cgagcgtgac
     2221 gaaggcggtg agcagcaccc cgccggtgaa ggcgaggaga agcaggatgc tgggaagggc
     2281 catgcgccgc ttccgggtga agaattcggc gaggccccaa gcggtggcgg cgacgaacag
     2341 gccggcgaag ggcgacggtc cgtcgccgcc gatgttcggc ccgatcgcgt ggccgatcca
     2401 gccgaccgcg
//
LOCUS       DASWNU010000498         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_19513, whole genome shotgun sequence.
ACCESSION   DASWNU010000498 DASWNU010000000
VERSION     DASWNU010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19513"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGB57_09805"
     CDS             complement(<1..194)
                     /locus_tag="VGB57_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0131688.1"
                     /translation="MALILCSPHNPTGTVPSRERLEAIAASAARHGSWVLADEIHAPL
                     TLPGAEHVPFLTVSEDAAARG"
     gene            complement(185..661)
                     /locus_tag="VGB57_09810"
     CDS             complement(185..661)
                     /locus_tag="VGB57_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0131689.1"
                     /translation="MNLSPDLTDLSLERLRRRRSAKWRQYPDDVLPAWVAEMDFPLAP
                     PVKQALREAIEVDDLGYPDPQDLGAAFAEFAERRHGWRPEPASVSPAPDVVGAITSVL
                     RALASPGDRVVVNTPVYHPFFAVIEEAGCELAEAPLVEGGLDPEAIDREFAAGPWR"
     gene            858..1136
                     /locus_tag="VGB57_09815"
     CDS             858..1136
                     /locus_tag="VGB57_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0131690.1"
                     /translation="MPRRDEPQIGLSRAIRQLRSEVHLSQEALGLRAGIHPTWISHME
                     SGRVNPTWGNVRRIALGLRVPLPELAALAEDLEAKHSTAPRSGEIGGR"
     gene            1242..>1846
                     /locus_tag="VGB57_09820"
     CDS             1242..>1846
                     /locus_tag="VGB57_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0131691.1"
                     /translation="MLRPVQKKLAFAIFLPVLLLLAGCGSSDDDTSTTASSCKPGQLP
                     TMQQGVLTIATDQPAYPPYFEDEDPTNGEGFESAVAYAIADQLGYPAEKVKWTVEPFN
                     SSYAPGPKGFDFDVNQISISPVREKAVDFSTPYYTANQAVVALKGSEAAAAKSLDELK
                     ETQIGVQIGTTSLDAVEAEIEPSEQPEVFNNSGDVVTALKN"
BASE COUNT          314 a          643 c          623 g          266 t
ORIGIN      
        1 ccgcgcgccg ccgcgtcctc cgacaccgtg aggaagggca cgtgctcggc acccggcagg
       61 gtgagcgggg cgtggatctc gtcggcgaga acccaggagc cgtgccgcgc cgccgaggcg
      121 gcgatcgcct ccagccgctc ccggctcggc acggtcccgg tcgggttgtg ggggctgcag
      181 aggatcagcg ccacggcccc gccgcgaact cgcggtcgat cgcctccggg tcgagcccgc
      241 cttcgaccag gggagcttcc gcgagttcgc agcccgcctc ctcgatcacg gcgaagaagg
      301 ggtggtagac gggcgtgttg acgacgacgc ggtcgccggg gctcgcgagc gcccgcagga
      361 ccgaggtgat cgcgccgacg acgtcggggg cgggggagac cgacgccggc tccggcctcc
      421 acccgtgccg gcgctcggcg aactcggcga aggccgcgcc caggtcttgc ggatcgggat
      481 agccgaggtc atcgacctcg atcgcctccc gcagcgcctg cttgaccggc ggcgccaacg
      541 ggaagtccat ctccgccacc caggccggca gcacgtcgtc ggggtactgc ctccacttgg
      601 cgctgcgccg ccgacgcagc cgctcgagcg agagatcggt gaggtcgggg gagaggttca
      661 tgcgccgccg aagtatctcc ggcggctcct ctcgcggctg atctgcccta tctcctagcg
      721 ggggcatatg cgaacatatg ttcgtgttcg tgatcgcgag agaccgcccc cttcaaccgg
      781 gtcctcgccc gcgacacagt cgtgagttga ggctgcctct atggacgcgg gggcgggagg
      841 cgcattccct tccctaaatg ccgcgacgtg acgaacccca gatcggcctg agcagggcaa
      901 ttcggcagct tcgcagtgaa gtccacctga gccaggaagc gctggggctg cgcgccggca
      961 tccacccgac ctggatctcg cacatggagt ccggccgggt caaccccacc tggggcaacg
     1021 ttcgccggat cgcgctcggc ctgcgggtgc cgctgcccga gctggcggcg ctggccgagg
     1081 atctcgaggc caaacactcg accgcccccc ggtccgggga gatcgggggg cggtagaaac
     1141 aaccttgggg tggttgtggt caactctata cccaatagta cctagatgag acacctatgg
     1201 ccgccattct ttgcggccgc ccgctccgat aggctcgccg cgtgcttcgc cccgtccaga
     1261 agaagctcgc cttcgcgatc ttcctgccgg tcctgctcct cctcgccggc tgcggcagca
     1321 gcgacgacga cacgagcacg acggcgagca gctgcaagcc gggccagttg ccgaccatgc
     1381 agcagggcgt gttgacgatc gccaccgatc agcccgccta cccgccttac ttcgaagacg
     1441 aagacccgac caacggcgaa ggcttcgaga gcgcggtcgc ctacgcgatc gccgaccagc
     1501 tcggctaccc ggcggagaag gtgaaatgga cggtggagcc gttcaactcc tcctatgcgc
     1561 cagggcccaa gggcttcgac ttcgacgtca accagatctc gatctcgccg gtgcgcgaaa
     1621 aagccgtcga cttctcgacc ccctactaca ccgccaacca ggcggtggtg gcgctgaagg
     1681 gatccgaggc cgccgcggcg aaatcgctgg acgagctgaa ggagacccag atcggggtcc
     1741 agatcggcac caccagcctc gacgcggtcg aagcggaaat cgaaccgagc gagcagccgg
     1801 aagtcttcaa caactcgggc gacgtcgtca cggcgctgaa aaacga
//
LOCUS       DASWNU010000499         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_304696, whole genome shotgun sequence.
ACCESSION   DASWNU010000499 DASWNU010000000
VERSION     DASWNU010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304696"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGB57_09825"
     CDS             complement(<1..281)
                     /locus_tag="VGB57_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEY0131692.1"
                     /translation="MSNIPTAAKRAVIHRMVMPSHTCPYGLKALHLLKRSGYEVEDRH
                     LTTREATDAFKAEHGVATTPQIFIAGRRIGGYDDLRRFLGKRVADPKAV"
     gene            complement(369..1250)
                     /locus_tag="VGB57_09830"
     CDS             complement(369..1250)
                     /locus_tag="VGB57_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131693.1"
                     /translation="MEEPSPRSSRLALAGALLAAIAVGGAGFLLGRSTSERPPAAIVA
                     APASAAPEPVAEPVVSGVLGRADLIALAAAAADAAAAGREPGPEIAQADGRRFELRLP
                     FGCTGPAVEDSAAGMRWRYDSEAQALRISVEPVAWAAQDWWPAGGAGDVEAIEGFWVA
                     RPWTSSEACPRGGDRAAVAGTEAVTLPGQTLAVGQIFLAGGGRGGRRDGKPYEAVVKV
                     AQDRLDSTGGFRLRISGRVARAGNAGPVRCRQPAGPEQRPICLVSVVMDEVAVENPAS
                     GGTLATWSLGTGKTPET"
     gene            1376..2713
                     /locus_tag="VGB57_09835"
     CDS             1376..2713
                     /locus_tag="VGB57_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0131694.1"
                     /translation="MNIDQILTLLVLAAVVAALIWDKVRPDVVALTGAAILLMTGVVR
                     PVEVQGAFASPAIIALASLFVIAYALELSGLLDRAIQLGVALCRRAGAAGIWILMAAI
                     GIVSCFLNSTPIVVLGAPVVRDIALTLKLSPKRYLMPLSYIAVLTGCCTLIGTSTNLL
                     VDDMARIAGQPRFGIFEITPVGLPMAIVGGLYLFFFSGRLMRRSLEEPMPRASPPRDD
                     VQAARAAMDDVHAGGIQVGHAEVFAEPRPFQPVKAMIAATIFVGAIVVAALNIAPIAA
                     SAFAGAVLLILLKVITADEAYGGLKPDILILIAGMVVIGIAMEESGLAGAATRAMLGS
                     LHGISPLVALILLYGATMILTELLSNATVAVLVTPIAVALAESLAVSPRPFLVAVMMA
                     GSAAFATPFGYQTNVIVYQMGGYRYMDFVRVGLPLNLITFATAIIAISLFFPF"
BASE COUNT          410 a         1027 c          934 g          487 t
ORIGIN      
        1 ctcaccgcct tgggatcggc gacgcgtttg ccgaggaagc ggcgaagatc gtcgtaaccg
       61 ccgatgcgcc ttccggcgat gaagatctgc ggcgtggtgg cgaccccatg ctccgcctta
      121 aacgcgtccg tcgcctcgcg cgtcgtcagg tggcgatcct cgacctcgta gccggagcgc
      181 ttcagaagat gcagcgcctt gagcccatag gggcaggtgt gggacggcat caccatccgg
      241 tggatcaccg cccttttcgc cgcggttgga atgttgctca tgtccgacat ctcacgtctc
      301 ctgtgaactt gccggtcctc atatagggtc cgtaccgtgg tacggagtca aggggtcgct
      361 gcccgaagtt atgtctccgg cgtcttgccg gtcccgaggc tccaggtggc caaggtcccg
      421 ccgctggccg gattctcgac cgcgacctcg tccatcacga cgctgacgag gcagatcggc
      481 cgctgctcgg ggcctgcggg ctgccggcac cggacggggc cggcattccc ggcgcgagcg
      541 acccgcccgc tgattcgaag gcgaaagccg ccggttgagt cgagccggtc ctgcgcgacc
      601 ttgaccacgg cctcataggg tttcccgtcg cgcctgccgc cgcgcccgcc gccggccagg
      661 aaaatctgcc ccaccgccaa tgtctgtccc ggcaaggtca ccgcctccgt tccggcaacg
      721 gccgcgcggt cgcctcctcg cgggcaggct tcgctcgatg tccaggggcg cgcgacccag
      781 aagccctcga tcgcctcgac gtcgccggcg ccaccggcgg gccaccaatc ctgagcggcc
      841 caggcgacgg gctcgacgga gatcctcagc gcctgggcct ctgaatcgta gcgccagcgc
      901 atccccgcgg cactgtcctc gacggccgga cccgtgcagc cgaacggcag ccgaagctcg
      961 aaccggcggc cgtcggcctg cgcgatctcg gggccgggtt cgcgacccgc ggccgccgcg
     1021 tcggcggccg cggcggcaag cgcgatcagg tcggcccggc cgagaacccc gctcacgacg
     1081 ggttccgcca ccggctccgg agccgcgctg gcgggggcag ccacgatcgc cgcgggcggg
     1141 cgctccgagg tggagcgtcc cagcaggaaa cccgcccctc ccaccgcgat cgcggccaaa
     1201 agggcgccgg cgagcgcgag tcggctgctc cgtgggcttg gctcctccat gggcacttga
     1261 taggcaaatg gcgccgaggc ctccagacct ttggaatcgc caggactgcc gcgacgcagg
     1321 aaggtccgat gcccgatcgc cctagccatt atccgatcgg gcgggcggcc tgccgatgaa
     1381 catcgaccag attctgacgc tcctcgtcct ggccgcggtg gtggcggctt tgatctggga
     1441 caaggtgcgg ccggacgtcg tcgctctgac cggggccgca atcctcctga tgaccggggt
     1501 cgtgcgcccg gtcgaggttc agggggcctt cgcaagcccg gccatcatcg cgctcgcctc
     1561 gctgttcgtg atcgcctacg cgctcgagct ctcggggctg ctcgatcgcg cgatccagct
     1621 cggggtcgcg ttgtgcaggc gcgccggggc ggccgggatc tggattctga tggccgccat
     1681 cggaatcgtt tcctgcttcc tcaacagcac gcctatcgtc gtgctcggag cgccggtggt
     1741 tcgcgacatc gcactcaccc tgaagctgtc gcccaagcgc tatctgatgc cgctctccta
     1801 tatcgccgtg ctcaccggct gctgcaccct gatcggcacc tcgaccaacc tgctggtcga
     1861 cgacatggcg cggatcgccg gccagccccg cttcgggatc ttcgagatca cgcccgtcgg
     1921 cctgccgatg gcgatcgtcg gcggtctcta cctgttcttc ttcagcggca ggctgatgcg
     1981 gcgaagcctg gaagagccga tgccgcgggc ttcgccgccc cgggacgacg tccaggccgc
     2041 cagggcggcg atggacgacg tccatgccgg cggaattcag gtgggccatg ccgaggtctt
     2101 cgccgagccc cggccgttcc agccggtcaa ggcgatgatc gcggcgacca tcttcgtcgg
     2161 cgccatcgtc gtcgctgcgt tgaacatcgc tccgatcgcc gcctcggcct ttgccggcgc
     2221 ggtgctgctc atattgctca aggtcatcac cgccgacgaa gcctatggcg gattgaagcc
     2281 cgacatcctg atcctcatcg ccggaatggt cgtgatcgga atcgcgatgg aggaaagcgg
     2341 gctcgccgga gcggccaccc gggcgatgct cggatcgctg cacgggatca gcccgctggt
     2401 cgcgctcatc ctcctctatg gcgcgacgat gatcctgacc gagttgctgt ccaacgcgac
     2461 cgtcgcggtg ctggtgactc cgatcgcggt cgcgctcgcc gaaagcctcg ccgtcagccc
     2521 acggccgttc ctggtcgccg tgatgatggc gggcagcgcc gccttcgcca cgcccttcgg
     2581 ctatcagacc aacgtcatcg tctaccagat gggcggctat cgctacatgg acttcgtccg
     2641 ggtgggcctg ccgctcaacc tgatcacctt cgcgaccgcc atcatcgcga tcagcctgtt
     2701 cttccccttc tgacccccgg cacgagcaag atcggtcggg caagcgccat actagcccct
     2761 tctatcccta gcgctcgtaa tcgagccggg ggcaagccgg agccggatca acggaaacgc
     2821 ccgcccttcg tcgtcgacgg gcttgcgggc gaccagtt
//
LOCUS       DASWNU010000500         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_279060, whole genome shotgun sequence.
ACCESSION   DASWNU010000500 DASWNU010000000
VERSION     DASWNU010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279060"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /gene="prsK"
                     /locus_tag="VGB57_09840"
     CDS             <1..1561
                     /gene="prsK"
                     /locus_tag="VGB57_09840"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEY0131695.1"
                     /translation="WASDLHLSTVGWLTRGPVDDLYAMRGAILAMLVPLFALASRRNA
                     RWRMRLSRAATFQSLSLIAILGYLILMMTASRALEVVGGNWVRIGQVGLVFAITVAAV
                     ILLPSGRMRAWSRVIVAKHFFEHRYDYREEWLRFTRTVGGDAPLGERIIKAIADIAQS
                     PSGLLLVPEGGRFSVSARWNWRDDLPEASADDAGLIRFVEASGHVLDFTGLSSGLLRT
                     DDAEVRVPAPLAGLDSLWAGVPLIHGDRLVGLVLLEHPLVRRPLDWEDFDLFRTVGIQ
                     AASYLAEAHTQDALADARRFDEFNRRFAFIIHDIKNLVSQLSLVVRNAERHADKPEFR
                     EDMIATLHSSVKKMNDLLARLHRGGANVEAEAVRAIPVGRAVSAVAEVKRRVHPVEVE
                     GDPTLAAVADPVRLEQALAHLVQNAIDASPGGAPVRMVCGRRGGEAAIEIADRGEGMS
                     ADFIRTRLFQPFASTKECGFGVGAFEARSLIAAMGGRIEVDSREGEGSRFTLFLPLGE
                     TSDLEIGRLRA"
     gene            1558..>2830
                     /gene="prsR"
                     /locus_tag="VGB57_09845"
     CDS             1558..>2830
                     /gene="prsR"
                     /locus_tag="VGB57_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEY0131696.1"
                     /translation="MSVDLRAKLLIVEDDEGLQRQLRWAYDDYEVLVASNRDAAINLL
                     RSEEPPVVTLDLGLPPDPNGVTEGFATLETILSLKPETKVIVASGHGARESALRAIAS
                     GAYDFYQKPVDIDQLGLIVRRAFQLHGIETENRKLEERAGEDQTVLGTMITSAPEMLK
                     VARTIERVANTGVSVMLLGASGTGKELLARGLHQASNRSGGGFVAINCAAIPENLLEA
                     ELFGYEKGAFTGAIKTTEGKIELAHGGTLFLDEVGDIPLPLQVKLLRFIQERVIERIG
                     GRKPIAVDTRIVSATHQDLPQMIKAGSFREDLYYRLAEIVVRIPSLAERHGDSVLLAK
                     HFLHRFAREMNPQVKGLSPAALAAIDEWPWPGNVRELENRMKRAVIMADGKLVTAEDL
                     DLEARTEAALPVNLKSARELADRRAIRQALAR"
BASE COUNT          453 a          954 c          979 g          444 t
ORIGIN      
        1 gtgggcctcc gatctccacc tctccacggt gggctggctg acccgggggc cggtcgacga
       61 cctctatgcc atgcgcggcg cgatcctcgc gatgctcgtg cccctgttcg ccctcgccag
      121 ccgccgcaac gcgcgctggc ggatgcgatt gtcgcgcgcc gccaccttcc agtcgctctc
      181 gctgatcgcg atcctgggct atctgatcct gatgatgacc gcgagccggg cgctcgaggt
      241 ggtcggcggg aactgggtcc ggatcggcca ggtcggcctc gtcttcgcga tcacggtcgc
      301 ggcggtgatc ctgcttccgt ccgggcggat gcgggcctgg tcgcgagtga tcgtcgccaa
      361 gcatttcttc gagcaccgct acgattatcg cgaggaatgg cttcgcttca cccgcaccgt
      421 cggcggcgac gccccgctcg gcgagcggat catcaaggcg atcgcggaca tcgcccaatc
      481 tccctcgggc ctgctgctcg ttccggaagg ggggcggttc agcgtctccg cccggtggaa
      541 ctggcgcgac gacctgcccg aggccagcgc cgacgacgcc gggctgatcc ggttcgtcga
      601 agccagcggc catgtgctgg acttcacggg cctgagctcg ggcctgctga ggaccgacga
      661 cgccgaggtg cgggtgccgg cgccgctggc gggactggac agcctttggg ccggagtgcc
      721 gctcatccac ggcgaccgcc tcgtcggcct ggtgctgctc gagcatccgc tcgtccggcg
      781 gccgctcgac tgggaggatt tcgacctgtt ccggacggtc gggatccagg ccgcgagcta
      841 tctggccgaa gcgcataccc aggacgcgct cgccgacgcc cggcgcttcg acgagttcaa
      901 ccggcgcttc gccttcatca tccacgacat caagaatctg gtcagccagc tcagcctcgt
      961 cgttcgaaac gccgagcggc acgcggacaa gccggagttc cgcgaggaca tgatcgccac
     1021 gctccacagc tcggtcaaga agatgaacga cctcctcgcg cgactccatc ggggcggcgc
     1081 caatgtcgaa gcggaggcgg tgcgggcgat cccggtcggg cgggcggttt cggcggtggc
     1141 ggaagtgaaa aggcgggtgc atccggttga ggtcgaaggc gatccgacgc ttgccgccgt
     1201 ggccgatccg gtccggctgg agcaggcgct cgcccatctc gtccagaacg ccatcgacgc
     1261 cagccccggc ggagcgcccg tcagaatggt ttgcggccgg cggggcgggg aagcggcgat
     1321 cgagatcgcc gaccgcggcg aaggcatgtc ggccgacttc atccgaaccc gcctcttcca
     1381 gcctttcgcc tcgaccaagg agtgcggctt cggagtcggc gccttcgagg cgcgaagcct
     1441 gatcgcggcc atgggcgggc ggatcgaggt cgacagccgc gaaggcgagg ggagccgctt
     1501 cacgctgttt ttaccgctgg gtgagaccag tgatttagag atcggacggt tgagggcatg
     1561 agcgtggact tgagggcgaa actgctgatc gtcgaggacg acgaggggct gcagcggcag
     1621 cttcgctggg cctatgacga ttatgaggtg ctggtcgcct ccaaccggga cgccgcgatc
     1681 aacctgctgc gctccgagga gccgcccgtc gttacccttg acctgggact gccgccggat
     1741 cccaacggag tcaccgaagg cttcgccacg ctggagacga tcctgtcgct caagccggag
     1801 accaaggtga tcgtcgcctc ggggcatggc gcccgcgaga gcgcgctccg ggcgatcgcg
     1861 tcgggcgcct acgatttcta ccagaagccg gtcgatatcg accagctcgg actgatcgtg
     1921 cgccgggcgt tccagctgca cggcatcgag accgagaacc gcaagctcga ggagcgggcg
     1981 ggcgaggacc agacggtgct cggcaccatg atcacctcgg cccccgagat gctgaaggtg
     2041 gcgcggacga tcgagcgggt cgccaatacc ggcgtttcgg tgatgctgct gggcgcgagc
     2101 ggcaccggca aggagctgct cgcccgaggt ctccaccagg ccagcaaccg ctccggcggc
     2161 ggcttcgtcg cgatcaactg cgcggcgatc ccggagaatc tgctcgaagc cgaattgttc
     2221 ggctacgaga agggcgcctt caccggcgcg atcaagacca ccgagggcaa gatcgagctc
     2281 gcccatggcg gaacgctctt cctcgacgag gtcggcgaca tcccgcttcc gctccaggtc
     2341 aagcttctgc gcttcatcca ggaacgggtg atcgagcgga tcggcggccg caagccgatc
     2401 gcggtcgaca ccaggatcgt cagcgcgacc caccaggacc tgccccagat gatcaaggcg
     2461 ggcagcttcc gcgaggatct ctattaccgg ctcgccgaga tcgtcgtccg gatcccgagc
     2521 ctggccgagc ggcacggcga ttcggtgctt ctcgccaagc atttccttca cagattcgcc
     2581 cgcgagatga acccgcaggt caaggggctc agcccggcgg cgctggcggc catcgacgaa
     2641 tggccgtggc cgggcaacgt ccgcgagctc gagaaccgga tgaagcgcgc cgtcatcatg
     2701 gccgacggca agctggtcac cgccgaggac ctcgatctcg aggcccggac cgaggcggcg
     2761 cttccggtca acctcaaatc ggcgcgcgag ctcgccgacc gccgggcgat ccgccaggcg
     2821 ctggcccgga
//
LOCUS       DASWNU010000501         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_459941, whole genome shotgun sequence.
ACCESSION   DASWNU010000501 DASWNU010000000
VERSION     DASWNU010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459941"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..759)
                     /locus_tag="VGB57_09850"
     CDS             complement(100..759)
                     /locus_tag="VGB57_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0131697.1"
                     /translation="MPVAISPVPGLEAPRDCPLCPRLVGFREALREEHPDWWNSPVPH
                     FGDPGAWLAICGLAPGKQGANRTGRPFTGDFAGLLLYETLLKFGLAEGRYEERPDDGL
                     RLTGVAIVNSVKCVPPQNKPTPDEIATCTRTYFQPSLAALSGVRVMVALGQIAHNAAV
                     RAVGGRLSAYRFGHLAEHRLPDGRLLIDSYHCSRYNQNTNRLTREMFEEVFARALELR
                     S"
     gene            783..1253
                     /gene="folK"
                     /locus_tag="VGB57_09855"
     CDS             783..1253
                     /gene="folK"
                     /locus_tag="VGB57_09855"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0131698.1"
                     /translation="MALTSYAIALGSNRRGRHGSPAATVRAAAEAIGAERMSKVRLTP
                     ALGPAGRGFANAVALVPSELDPPALLARLKQVERDFGRRGGRRWGPRILDLDIILWSQ
                     GAWAGDGLIVPHPEFRRRAFVLEPLAEIAPGWRDPITGATVRQLLFRLGRRSEA"
     gene            complement(1343..2176)
                     /locus_tag="VGB57_09860"
     CDS             complement(1343..2176)
                     /locus_tag="VGB57_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0131699.1"
                     /translation="MRRSFVTPEGVDLQLELGAAGARAGAFLLDAMMIVTILVLATIA
                     LAFLALASKAEWVIILWLLGFFVLRNGWFSLFEMGGRGATPGKRLMGLRVVARDGARL
                     TGGAVIARNAMREIEVFLPLSFLAEQTAQGTADAFLVLFSLVWSGIFLFFPLFNRDRL
                     RIGDLIAGTWVVRTAGASLGADLVHEAERSRRTFTEAALNLYGVYELQTLEDVLRAGR
                     DDAIATVARTIRDKAGIEEWGNDREFLSDYYAALCARLESGLMVGRRRENKFEQALTR
                     R"
     gene            complement(2196..3233)
                     /locus_tag="VGB57_09865"
     CDS             complement(2196..3233)
                     /locus_tag="VGB57_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY0131700.1"
                     /translation="MSAPASAIVESRTVVVNASRFRAAHEADWERLERLVSLMEKRSI
                     RALSDDDILALPLLYRTTLSSLAVARDTSLDRGLVVYLEQLSTRAYFQIYGVQTSVWR
                     QLGHFFARGWPEAVQSLWRETLVCVFLTLASAVVAFLLVRGDPSWFFGIIPESLAGGR
                     DPSASAEALSKTLYDRQNDMLATFATYLFTHNSQVAIFAFALGFAFAVPTILLILYNG
                     LMLGAFFAVFAAKGLAFNLGGWLAIHGTTELFAIAIAGAAGLRIGMAIAFPGRETRTE
                     SAVRAGRSAGLAMAGTVLMLAVAGLLEGIGRQTIVDDFSRYAIGAAMLAGWLTYFYMP
                     RKGRDAPVVAQ"
     gene            complement(3230..4540)
                     /locus_tag="VGB57_09870"
     CDS             complement(3230..4540)
                     /locus_tag="VGB57_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0131701.1"
                     /translation="MIYPTRMAVLAAAAGVPFTLVVAAAMPERWYGGLAWPVAVLLLT
                     LVDAFTAVRHGEAKLDAPATAYVGSTVEALVTVTLRGDAAPAEAEVAVAANPLVGLED
                     DGRLWIALEQGRGAAPLLLAMARRGTARLERLWLRWRGPLGFAWQQRQIELDRAILIL
                     PDVRPVQEHGAQVFQRHALQGLIAQINRGDGTDFDALVEYRSGMDRRAIDWKQSARHL
                     KLHAKEFRSERNNQIVFAIDGGRQMSEPVAGLPRVDRAVSAMLLTGWVALKLGDRVAL
                     HAFDSRPRIASGLISGSAAFPELQRLAASIDYSGEETNYTFALTTLATRLTRRSMIVL
                     FTEFTDLTSADFMVRAARRMVETHLLLVVVLRDEELEAIADAMPARPEDVTRAVTAAA
                     LIRDRRLALTRLQHLGVHIIESEYDRVGERLVAGYVDLKRRNLL"
     gene            complement(4537..>4946)
                     /locus_tag="VGB57_09875"
     CDS             complement(4537..>4946)
                     /locus_tag="VGB57_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY0131702.1"
                     /translation="RIIATHGARQSAMEPVQWGVERKADSAAIGAAVATVGEVKLVDE
                     VIDYISALIRGTRDVADLETGASPRAGAMLAGAARARAAIDGRDFVIPDDVKTLAPAL
                     LRHRLILSPAAEINGRVVEDVVTDIIETIEAPR"
BASE COUNT          821 a         1677 c         1751 g          697 t
ORIGIN      
        1 cacgcaggcg gaagaggaac agcgcgcctt cggcctcggc ttcggcgagc agcggttcga
       61 acaaggcctg cgaatattcg agcgggcgga tctccacggt cacgagcgca gctccagcgc
      121 cctggcaaac acttcctcga acatctcccg ggtcagccgg ttggtattct ggttgtagcg
      181 cgagcaatgg tagctatcga tgagcaggcg gccgtcgggc aggcggtgct ccgcgaggtg
      241 gccgaagcga taggcggaga gccggccgcc gaccgcccgg accgcggcat tgtgagcgat
      301 ctggccgagc gcgaccatca ccctcacccc ggacagtgcc gccagcgacg gctggaaata
      361 ggttcgggtg caggtggcga tctcgtcggg cgtcggcttg ttctgcgggg ggacacattt
      421 gaccgaattg acgatggcca ctccggtgag ccgaagcccg tcgtcgggac gttcctcgta
      481 ccgtccctcg gcgaggccga acttgagcag ggtctcgtag aggaggaggc ccgcgaaatc
      541 cccagtgaag ggccgcccgg tgcggttggc gccctgcttg ccgggggcga ggccgcaaat
      601 cgccagccag gcgccgggat cgccgaaatg cgggacgggg gaattccacc agtccggatg
      661 ctcctcgcgc agcgcctcgc ggaaacccac gaggcgcggg cagagcgggc agtcgcgcgg
      721 ggcttcgagc ccggggacgg gagagatggc aacgggcatc gccagcgtct agggcggccg
      781 cgatggcgct gacaagctac gcgatcgccc tcggctccaa ccgccggggc cgccacggtt
      841 cgccggccgc caccgtgcgg gcggcggcgg aagcgatcgg agcggagcgc atgtcgaagg
      901 ttcggctcac ccccgccctc ggtccggcag gccgcggctt cgccaatgcc gtcgcgctcg
      961 tcccgagcga gctcgacccc cctgccctgc tcgcccgcct caagcaggtg gagcgcgatt
     1021 tcggccggcg cggcggccgg cgctggggcc cccggatcct cgacctcgac ataatcctgt
     1081 ggtcgcaagg cgcctgggcg ggagacggcc tcatcgtccc ccatcccgaa ttccggcgcc
     1141 gcgctttcgt gctcgagcca ctcgccgaga tcgcgcccgg ctggcgcgac ccgatcaccg
     1201 gcgccaccgt ccggcaactg ctctttcggc tgggacggcg atccgaggcc taaccgtcat
     1261 cccggcgcaa gccgggacct caggacaaga agtcacggcg ggtctccacg aggtcccggc
     1321 ttccgccggg atgacgggca ggtcagcgcc gcgtgagcgc ctgctcgaac ttgttctccc
     1381 ggcgccggcc gaccatcagc ccgctttcga ggcgggcgca gagcgccgcg taataatcgg
     1441 acaggaattc gcggtcgttg ccccactctt cgatccctgc tttgtcgcgg atcgttcggg
     1501 cgacggtggc gatcgcgtcg tcgcggccgg cgcgcaggac gtcctcgagc gtctggagct
     1561 catagactcc gtagaggttg agcgccgcct cggtgaaggt ccgccgcgac cgctcggcct
     1621 cgtggacgag atcggcaccg agcgaggctc cggccgtcct caccacccag gtgccggcga
     1681 tgaggtcgcc gatccggagc cggtcgcggt tgaagagcgg aaagaacagg aagatgccgc
     1741 tccacaccag ggagaagagt accaggaagg cgtcggcggt gccctgggcg gtctgttcag
     1801 cgaggaagga caagggcagg aagacctcga tctcgcgcat cgcgttgcgg gcgatcaccg
     1861 ctccgccggt gagccgtgcg ccgtcccggg cgacgacccg aaggcccatc agcctcttgc
     1921 cgggagtggc gccgcggccg cccatctcga acagcgagaa ccagccgttg cgaaggacga
     1981 agaagccgag cagccacagg atgatgaccc attccgcctt cgacgcgagc gccagaaagg
     2041 ccaaggcgat cgtcgccagg accaggatgg tgacgatcat catcgcgtcg aggaggaagg
     2101 ccccggcgcg ggcgccggcg gcgccgagct cgagctgaag gtcgactcct tcgggcgtga
     2161 cgaagctgcg ccgcatcgag gccggaaggg cctccttatt gcgcgacgac gggcgcatcg
     2221 cgtcccttcc gcggcatgta gaaataggtc agccagcccg ccagcatcgc ggcgccgatc
     2281 gcgtagcggg aaaaatcgtc gacgatcgtc tggcggccga tcccctcgag cagcccggcc
     2341 accgccagca tcagcaccgt tccggccatt gcgagccccg ccgagcggcc cgctctgacg
     2401 gcggattcgg ttcgggtctc ccgtcctgga aaggcgatcg ccattccgat ccgaagcccc
     2461 gcggcgccgg cgatggcgat ggcgaacaat tcggtcgtgc cgtgaatcgc cagccagccg
     2521 ccgaggttga aagcgagccc cttcgccgcg aagaccgcga aaaaggcgcc gagcatcagg
     2581 ccgttgtaga ggatgaggag gatggtcggc accgcgaagg cgaagccgag cgcgaaggcg
     2641 aagatcgcga cctggctgtt atgggtgaac agataggtgg cgaaggtggc gagcatgtcg
     2701 ttctggcgat cgtagagcgt cttgctcagc gcctcggcgc tggccgacgg atcgcgtccc
     2761 cccgcgagcg attccgggat gatcccgaag aaccagctcg gatcgccgcg caccagcagg
     2821 aaggcgacga cggcgctcgc cagggtgaga aagacgcaga ccagggtctc gcgccaaagg
     2881 ctctgaaccg cctccggcca gccgcgggcg aagaaatggc ccagctggcg ccagacggag
     2941 gtctggactc cgtagatctg gaaataggcg cgggtgctga gctgctcgag atagacgaca
     3001 aggccgcggt cgagcgaggt gtcgcgcgcc accgcgagcg aggacaaggt ggtccggtag
     3061 agcaaaggca gagcgaggat gtcgtcgtcg gagagtgccc ggatcgacct cttctccatc
     3121 aggctcacca gccgctcgag ccgctcccaa tccgcctcgt gcgcggcccg gaaacggctg
     3181 gcgttgacca ccaccgtccg gctttcgacg atcgccgaag ccggagcgct cacagcaaat
     3241 tcctccgctt gagatcgaca tagccggcga ccagccgctc gccgacccga tcatattcgc
     3301 tttcgatgat atggactccg agatgctgaa gccgggtcag agcgagccgg cggtcccgga
     3361 tgagcgccgc cgccgtgacc gcccgcgtga cgtcttcggg cctggccggc atcgcgtcgg
     3421 cgatcgcctc cagctcctcg tcgcggagga cgacgacgag caggagatgg gtctcgacca
     3481 ttcgcctggc cgcgcggacc atgaagtcgg ccgaggtgag atcggtgaat tcggtgaaca
     3541 ggacgatcat cgaccggcgg gtgagtcggg tggcgagagt ggtcagagcg aaggtgtagt
     3601 tggtctcctc gccgctgtag tcgatgctcg cggcgagccg ctggagctcc gggaaggcgg
     3661 ccgaaccgga gatcagcccg ctggcgattc ggggcctcga atcgaaggcg tgaagagcga
     3721 cccggtcgcc gagcttgagc gcgacccagc cggtgagaag catcgccgag accgcccggt
     3781 cgacccgcgg cagccccgcc accggctccg acatctgccg cccgccgtcg atcgcgaaga
     3841 cgatctggtt gttgcgctcg ctgcggaatt ccttggcgtg gagcttcaga tgccgggccg
     3901 attgcttcca gtcgatcgcc cggcggtcca tgcccgagcg atattcgacg agggcgtcga
     3961 aatcggttcc gtcgccgcgg ttgatctggg cgatcaggcc ctgcagcgcg tggcgctgga
     4021 agacctgggc gccatgctcc tgcaccggcc ggacgtcggg caggatcagg atggccctgt
     4081 cgagctctat ctgcctttgc tgccaggcga agccgagcgg cccgcgccag cggagccaca
     4141 atcgctccag ccgggcggtg ccgcgccggg ccatggccag gagcagcggc gccgcccccc
     4201 tgccctgctc gagcgcgatc cacagccgcc cgtcatcctc gagaccgaca agggggttgg
     4261 cggcgaccgc cacctcggcc tcggccggag ccgcgtctcc cctgagagtg actgtcacca
     4321 gcgcctcgac ggtcgagccg acataggcgg tggcgggagc gtccagcttc gcctcgccat
     4381 ggcggacggc ggtgaaggcg tcgaccaggg tcagaagcag caccgccacc ggccaggcga
     4441 ggccgccata ccagcgctcc ggcatggccg cggcgacgac cagggtgaac ggcaccccgg
     4501 ccgcggccgc gagcaccgcc attcgggtcg gatagatcaa cgcggcgcct cgatcgtctc
     4561 gatgatatcg gtcaccacgt cctcgacgac ccggccgttg atctcggcgg ccggcgacag
     4621 gatcaggcgg tggcggagca gcgccggggc gagggtcttg acgtcgtcgg ggatgacgaa
     4681 gtcgcggccg tcgatcgcgg cccgggcccg ggcggcgccg gcgagcatcg ccccggcgcg
     4741 cgggcttgcc ccggtctcga gatcggcgac gtcgcgggtg ccccggatca gggcggagat
     4801 atagtcgatc acctcgtcga ccagcttcac ttcgcccacc gtcgccaccg ccgctccgat
     4861 cgcggcggaa tccgccttgc gctcgacgcc ccattgcacc ggctccatcg ccgactggcg
     4921 ggcgccgtgg gtggcgatga tccgcc
//
LOCUS       DASWNU010000502         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_97474, whole genome shotgun sequence.
ACCESSION   DASWNU010000502 DASWNU010000000
VERSION     DASWNU010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97474"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGB57_09880"
     CDS             complement(<1..185)
                     /locus_tag="VGB57_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY0131703.1"
                     /translation="MRDDTRNDSTPAKLYAGLIGATLLVAGIIGFFYSSAFGSPGEVD
                     AVFGILDVNGWHNLVHIA"
     gene            complement(260..817)
                     /locus_tag="VGB57_09885"
     CDS             complement(260..817)
                     /locus_tag="VGB57_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131704.1"
                     /translation="MFEREAEVLVRLSRALGLPEWVPEPYGLAIRFPDAYGPDAHQDF
                     LLVTSASPPGGRHTLLPARGFCDLPYSTVLPYRLRGEMALIGARAPAPGPGPKLGDLR
                     ERTVGGLEFELAIAGITGAWKPVARLALGERLPPEQTEHLHFDPTNTGGGLELAGLLN
                     RLRGPAYSASQEGREDAYKRFGLPH"
     gene            complement(967..>1606)
                     /locus_tag="VGB57_09890"
     CDS             complement(967..>1606)
                     /locus_tag="VGB57_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY0131705.1"
                     /translation="PQLPEGFVFDSELDPAAHSGFVDALYVSLVNLTSLGYGDISPGT
                     SLLQLLGPLETIFGLGLLTAGISWLISIYNAISRRDSLAHEVHLAKETEDRLGEKLAD
                     ADPELLESLLAGFAEQLIRVRRDLIHFPITHYFRTEDEERALAGLLPFLGSLVDEAGG
                     EERPHSLRVRAEILRMAIDDFAETLRVRLRLPGESTAATLEHYQSEHGPLRS"
BASE COUNT          261 a          546 c          577 g          222 t
ORIGIN      
        1 gcgatgtgga cgaggttgtg ccagccgttg acgtcgagga tgccgaacac cgcgtcgact
       61 tcgccgggac tgccgaaggc ggagctgtag aagaagccga tgatcccggc gaccaacagg
      121 gtggcgccga tcaagccggc atagagcttg gcgggggtgg aatcgttgcg agtgtcgtca
      181 cgcatgtatt gggctcctcc taagagagct ggttggacga gaactcctac gtagagctac
      241 cccagacaga tcacctcaat cagtgaggaa gtccaaaccg tttgtaggcg tcctcgcggc
      301 cttcctggct ggcgctgtaa gcgggtccgc gcaggcggtt gagcaggccg gccaactcca
      361 gcccgccgcc ggtgttggtg gggtcgaagt ggaggtgctc ggtctgctcc ggcggcagcc
      421 gctcccccag cgccagccgc gccaccggct tccaggcgcc ggtgatcccg gcgatcgcga
      481 gctcgaactc gaggccgccg acggtgcgct cgcgcaggtc tcccaacttc ggccccgggc
      541 cgggggccgg cgcccgcgcc ccgatcaacg ccatctctcc ccgcaggcgg tatggaagga
      601 ccgtcgagta ggggaggtcg cagaagccgc gagcggggag cagggtgtgg cggccgccgg
      661 gcggcgaggc cgaggtgacc aggaggaagt cctggtgggc atcgggcccg taggcgtccg
      721 ggaaccggat cgccagccca tagggctccg gcacccactc cggcaggccg agggcacgcg
      781 agagccgcac cagcacctcg gcctcccttt cgaacacggc cgcgccgcag ccggtccctc
      841 cctgtggcgt caggcgggcg gagaacccca ccccgaacgg gtggaaccca cgcttgcgcc
      901 gcagcttcga gagcgtccga taagggttca ctccgcctga ctacccgcac cgctcgaagc
      961 ggaagctcag gagcgcaggg ggccgtgctc ggactggtag tgctcgagcg tggctgcggt
     1021 gctctcgccc ggcagccgca gccgcacccg cagcgtctcg gcgaagtcgt cgatcgccat
     1081 ccgcaggatc tcggcccgca cccgcagcga gtgcggccgc tcctctcccc ccgcctcgtc
     1141 caccagcgag ccgaggaacg gcagcagccc ggccagcgcc cgctcctcgt cctcggtgcg
     1201 gaagtagtgg gtgatcggga agtggatgag gtcgcggcgg acgcggatca gctgctcggc
     1261 gaatccggcc agcagcgact ccagcagctc cggatcggcg tcggccagct tctcccccag
     1321 gcggtcctcg gtctccttcg ccaggtggac ctcgtgggcc agcgaatcgc ggcgggagat
     1381 cgcgttgtag atcgagatca gccaggagat gcccgcggtg agcaggccga gcccgaagat
     1441 cgtctccagc ggccccagca gctgcagcag cgacgtcccg ggcgagatgt cgccgtatcc
     1501 gaggctggtg aggttcacca gcgagacgta gagggcgtcg acgaagcccg agtgcgccgc
     1561 cgggtcgagc tcggagtcga agacgaagcc ctcgggcagc tgcggc
//
LOCUS       DASWNU010000503         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_45511, whole genome shotgun sequence.
ACCESSION   DASWNU010000503 DASWNU010000000
VERSION     DASWNU010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45511"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1054)
                     /gene="hemE"
                     /locus_tag="VGB57_09895"
     CDS             complement(29..1054)
                     /gene="hemE"
                     /locus_tag="VGB57_09895"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0131706.1"
                     /translation="MAVADKPLLSVLSGRKAEKTPLWLMRQAGRYLPEYRALRADKGG
                     FLDLVQDPDAAAEVTLQPIRRFGFDGAILFSDILMVPFALGQDLRFEAGEGPRLSPPL
                     VDSALAGLDSAPGRLEPVYRTVEKVAQCLPEATTFLGFAGSPWTVATYMVAGQGSKDQ
                     GEARRLAYGDPAAFQAIVDTISGLTVDYLSRQVESGVEAVQLFDSWAGSLSPAQFERW
                     VIAPTAALVAAFKARHPETPVIGFPKGAGGKLAAYALETGVDAIGLDETVDPVWADSV
                     LPPGMPVQGNLDPLALIAGGQALETAVGRILSSLAGRPHIFNLGHGILPDTPIQHVSD
                     LLALVRQ"
     gene            1367..2209
                     /locus_tag="VGB57_09900"
     CDS             1367..2209
                     /locus_tag="VGB57_09900"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY0131707.1"
                     /translation="MTTLHLHLLSDSTGETLEVIATACLAQFEGVDVRRHLWPMVRSE
                     GHLDRVLDDVERRPGLVLYTLVNKTLRRDLEQKTRRRGIHAVAALDPVTHALSVVLNR
                     EALGRPGRQHVLDAAYFARVDAIQFTIAHDDGIGSDNWEEADIVLAGVSRTSKTPTSI
                     YLANRGYKVANIPLVPEAPPPPSLFSLEHPLVVGLTTNPDRLVQIRRNRLLALNQAPE
                     TDYVDIEAVNAELAFARRLFADNDWPVIDVTRRSIEETAFAIVKLCNERMDAAGAPGG
                     CKST"
BASE COUNT          387 a          760 c          699 g          380 t
ORIGIN      
        1 tgaagagccg ttgcgaaatc atcggtgatc actggcgcac cagggcgagc agatcggaaa
       61 cgtgctggat cggcgtgtcc ggcaagatgc catgccccag gttgaagata tgcggtctcc
      121 ccgcaaggct agataggatg cgtcccacgg cggtttcaag cgcctggccg ccggcgatca
      181 gggccagcgg gtcgagattg ccctggacgg gcatgcccgg cggcaggacg gagtccgccc
      241 agaccgggtc gaccgtctcg tcgagaccga tcgcgtccac gccggtttcg agcgcataag
      301 cggcgagctt gccgccggcg cccttgggaa agccgatgac gggcgtttcc ggatgcctgg
      361 ccttgaaggc ggcgacgagc gccgcggtcg gcgcgatcac ccagcgctcg aactgcgccg
      421 gcgaaaggct tccggcccag ctgtcgaaca attgcaccgc ctcgacccca ctctccacct
      481 ggcgcgagag atagtcgacg gtgaggccgg aaatggtgtc cacgatcgcc tggaaggccg
      541 ctggatcgcc gtaggcgagc cttcgcgcct ccccctggtc cttgctgccc tggcccgcga
      601 ccatgtaggt ggcgacggtc caggggctgc ccgcgaaacc gaggaaagtc gtagcttccg
      661 ggagacactg tgcgactttc tccacagtcc ggtagacggg ctccagacgc cccggggccg
      721 agtcgagccc ggcaagcgcc gagtcgacca gaggcggcga caggcgcgga ccctcgccgg
      781 cctcgaagcg cagatcctgg cccaaagcga agggcaccat gaggatgtcg gagaagagga
      841 tcgccccgtc gaagccgaag cgccggatcg gctggagagt cacctcggcc gcggcgtccg
      901 ggtcctggac gaggtcgagg aagccgcctt tgtccgccct caacgccctg tattcgggca
      961 ggtagcggcc ggcctggcgc atcagccaaa gcggagtttt ctcagctttt cggccgctta
     1021 gcacgctgag cagaggcttg tcggcgacgg ccatggacaa atgaggctcc gctctactct
     1081 ctataagatt cttatataaa gagagtctgt tgtaggttgt aggaaggcgc gtcgcggggt
     1141 ttattggctt cgcccgattt tgcaaacagc ttgactctca acgcatggcg gaagcccgcg
     1201 gattcgcgat atccatcccc gctatccaca gcttgcaggt aggaactttt tcacctttgc
     1261 gactctgtgg acgaaatccg gattgcgggg gcaaaccgtt cgcttgcaac gctcccgagg
     1321 cttaagctag cgattcccac atgcttatcc acaggcggtt tcagggttga ccacgctcca
     1381 cctccatctc ctgtcggatt cgaccggtga gactctggag gtcatcgcca ccgcctgcct
     1441 cgcccagttc gagggcgtcg acgtccgccg ccacctgtgg ccgatggtgc gctccgaagg
     1501 ccatctcgat cgggtcctcg acgacgtcga gcggcggccg ggcctcgtcc tctacacgct
     1561 ggtgaacaag actctccggc gcgatctcga gcagaagacg cgccggcgcg ggatccatgc
     1621 cgtcgccgcc ctcgatcccg tcacgcacgc gctatccgtc gtcctcaatc gcgaagccct
     1681 gggccggccg ggccgccagc acgtgctcga tgccgcctat ttcgcgcggg tcgacgcgat
     1741 ccagttcacc atcgcccatg acgacggcat cggctccgac aattgggagg aggcggacat
     1801 cgtcctcgcc ggggtctcgc gcacgtccaa gaccccgacc tccatctatc tcgccaatcg
     1861 cggctacaag gtcgccaata tcccgctcgt tccggaagcg ccgccgccgc cttcgctctt
     1921 ctcgctcgag catccgctcg tcgtcggcct caccaccaat ccggaccggc tggtccagat
     1981 ccgccgcaac cgcctgctcg cgctcaacca ggcgcccgag acggactatg tcgacatcga
     2041 ggcggtcaat gccgagctcg ccttcgcccg acgcctgttc gccgacaacg actggccggt
     2101 gatcgacgtc acccgccgct cgatcgagga gacggcgttc gccatcgtca aattgtgcaa
     2161 cgagcggatg gacgccgccg gcgcccccgg cggctgcaaa agcacgtgaa gctcctgctc
     2221 gcctcg
//
LOCUS       DASWNU010000504         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_512732, whole genome shotgun sequence.
ACCESSION   DASWNU010000504 DASWNU010000000
VERSION     DASWNU010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512732"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VGB57_09905"
     CDS             complement(<1..1493)
                     /locus_tag="VGB57_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131708.1"
                     /translation="MKLAWLALLLCAPAAARAEWHEASSRHFLVYSEGSAQSVREFAT
                     KLEKFDKAMRLRLGFQDEDLGPGNRVTVFVVDNMAAVQRLARARGSGIAGFYSGRAGT
                     SVAVVPRTSGSGRVTDLDPATILLHEYSHHLMLQNVAAAYPVWFREGFAEFYSTARFE
                     KDGTIGFGAAANHRAWGLVGMSPLPIELLFDPTRRKLSQAEWESTIYGRGWLLTHYLT
                     FDRTRKGQLSAYLNAIQSGKKSLEAAQSAFGDLKVLDRELKTYLNRRTIPYLPMKAEL
                     LTIQPVTLRTLRPGEAAIMDLRMRSRVGVTKEDAARVARDMRKAAAAWPDDPTVQAAL
                     AEAEFDAGDLSAAEAAAARAIAADPKNVDALTYRAMVAFERAKASTADGEAVWKAVRG
                     LIGKANRADANDPRPLILYYRSFGEQGVAPTRLAVDGLLQAFALAPQDGGLRMNSARQ
                     LLVDGKAADARAALDPIAYDPHGGALGQAVAAIVAAIDKDGAGAALRS"
     gene            complement(1589..3115)
                     /locus_tag="VGB57_09910"
     CDS             complement(1589..3115)
                     /locus_tag="VGB57_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131709.1"
                     /translation="MDRLLKFVWLTLLLLAPQAAQAEWRAAETRHFTFYSESGDRGIE
                     KLAARLESYDKLMRMATGISDEAEPVKVRIYEVDSLREVEQALGLSDSGIAGFYDSNI
                     LGPFAVTPRETLNDLHFTPALVLHHEYAHHFMLQYFPSIYPPWYVEGFAELIGSSKVL
                     ADGKIGYGMPARHRGGQIAVDWVNVADLLLKPPHKLRFLDLYGQGWALTHFLTFSKER
                     SPQMRAYLEALRAGKPAEEAAKAFGDLKALDREARRYVTTGAFDYRPVPVEIARPAIK
                     SLRVLSPGEAASIPTVIALRDDELSLYRKAGAREREAKLRAANLERIRESARRFARDP
                     FVLHLLAAAEFAAGNHSASEAAADRLLVLQPGHARGMALKSLNMARAAGALQGAARRA
                     RAAEARALATRANRADPDDTLPLLAFYESFHLAGEKPSKEAVNVLAAVVATQPGDTKA
                     RQLLVDQLAADARWALAIDTLRPIANSPHESPRREAAREQLAKLEAELAKAGAAAPSS
                     "
     gene            complement(3184..>4224)
                     /gene="priA"
                     /locus_tag="VGB57_09915"
     CDS             complement(3184..>4224)
                     /gene="priA"
                     /locus_tag="VGB57_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0131710.1"
                     /translation="AELPDIEAIDLIAEPPPRGRWIAPRLVSEIKETLERGEQALLFL
                     NRRGYAPLTLCRACGHRFQCPNCTAWMVEHRLVHRLACHHCGHMMPPPQACPECGEEG
                     SLVPVGPGVERIADEVAALFPDARTAVVTSDTIWSPAKAAEFVGRMEAGEIDIVVGTQ
                     LVTKGYHFPNLTLVGVVDADLGLQGGDLRAAERTFQQIAQVAGRAGRGEKPGRVLVQT
                     HEPSAAVIEALVSGDSESFYAAETQSRREAAMPPFGRLAGIVISSEDPGEATEAAKLI
                     GRAAPRVENMAVFGPAPAPLAMLRGRHRQRLLVHAARSVDMQEVIRDWLGKLSWPRGV
                     RVTVDVDPYSFL"
BASE COUNT          635 a         1449 c         1471 g          669 t
ORIGIN      
        1 gagcggagcg cggcgccagc gccgtccttg tcgatggccg ctacgatcgc tgcgaccgcc
       61 tgtcccagcg cgccgccatg cgggtcatag gcgatcgggt caagcgccgc ccgagcgtcc
      121 gcggccttgc cgtcgacaag cagctggcgg gcggagttca tcctcaggcc tccgtcctgc
      181 ggagccaggg cgaacgcctg aagcagtccg tcgaccgcga gcctggtcgg cgcgacgccc
      241 tgctcgccga agcttcgata gtaaaggatg agcggcctcg gatcgttcgc atcggcgcgg
      301 ttggccttgc cgatcaggcc gcgcacggcc ttccagacgg cctcgccgtc cgcggtggag
      361 gccttggcgc gctcgaaggc caccatggcg cggtaggtca aggcgtcgac attcttcggg
      421 tcggccgcga tcgcccgggc ggcggccgcc tccgcggcgc tgagatcgcc cgcgtcgaat
      481 tcggcttccg cgagcgcggc ctggacggtg gggtcgtccg gccaggccgc cgccgccttg
      541 cgcatgtcgc gcgcgactcg ggccgcatct tccttggtca ctccgactcg ggagcgcatt
      601 ctgaggtcca tgatcgccgc ctccccgggc cgcaacgtcc tcagggtcac cggctggatc
      661 gtcagcagct ccgccttcat cggcagatag gggatggtgc gacggttgag ataggtcttc
      721 agctcccggt cgagcacctt gaggtcgccg aaggccgatt gcgccgcctc gaggctcttc
      781 ttgccgctct ggatggcgtt cagataggcg gagagctgcc ccttccgggt ccggtcgaac
      841 gtcagatagt gggtgaggag ccagccgcgc ccatagatgg tcgactccca ttccgcctgg
      901 ctgagcttgc ggcgggtcgg atcgaacaga agctcgatcg gcagcgggct catcccgacg
      961 aggccccagg cgcggtggtt ggccgcggcg ccgaagccga tcgtcccgtc cttctcgaac
     1021 ctcgcggtgg agtagaattc ggcgaagcct tcgcggaacc agaccggata ggcggcggcc
     1081 acgttctgga gcatcaggtg atgcgaatat tcgtggagca ggatggtcgc cggatcgaga
     1141 tcggtcactc gtccagaccc gctggtcctg gggacgaccg ccaccgaggt gccggcgcgg
     1201 ccgctgtaga agccggcaat gccggacccc cgcgcccggg cgaggcgctg caccgcggcc
     1261 atattgtcga ccacgaacac cgtcacccga ttgccgggtc ccagatcctc gtcctggaaa
     1321 ccgaggcgaa ggcgcatcgc cttgtcgaac ttctcgagct tcgtcgcgaa ctcccggacc
     1381 gactgcgcgc tgccttcgga atagacgagg aaatgcctgc tgctcgcctc gtgccactcg
     1441 gcccgagccg cggccggggc gcaaagcagc agggccagcc aggccagttt catcagccgg
     1501 ttcatcatcc ccaccgtttt tgttttgcga agcagaagct tagccgaacc ggcggagaat
     1561 ggcaaagtcc ctcccggcgc gcgcgtcatc aggaggacgg cgccgcggcg ccggccttgg
     1621 cgagctccgc ttccagcttc gccagctgct cccgggccgc ctcgcgcctc ggcgattcgt
     1681 ggggtgagtt ggcgatcggc cggagcgtgt cgatggccag cgcccaccgg gcatcggcgg
     1741 cgagctggtc gaccagcagc tggcgcgcct tggtatcgcc cggctgcgtc gcgacgaccg
     1801 cggcgaggac gttgacggcc tctttcgacg gcttctcgcc ggcgaggtgg aagctctcgt
     1861 agaaggcgag caggggcagc gtgtcgtcgg gatcggcgcg gttcgccctg gtcgccaggg
     1921 cgcgcgcttc cgcggccctc gcccttcggg cggcgccctg cagggcgccc gcggcgcgtg
     1981 ccatgttcaa cgacttcagc gccatcccgc gcgcgtgccc cggctgcaga acgagcagcc
     2041 ggtcggccgc cgcctcggag gccgagtgat tgccggcggc gaattcggcc gcggcgagca
     2101 gatggagcac gaacgggtct ctcgcgaacc ggcgggcgct ctcccggatc cgctccaggt
     2161 tcgccgcccg gagcttcgcc tcgcgctcgc gtgcgccagc cttgcggtag agcgaaagct
     2221 cgtcgtcgcg cagggcgatc acggtcggga tcgaggccgc ttcccccggg ctcagcaccc
     2281 ggaggctctt gatggcgggg cgggcgatct cgaccgggac cgggcgatag tcgaacgcgc
     2341 cggtcgtgac atagcgccgc gcctcccggt cgagcgcctt tagatcgccg aacgccttcg
     2401 ccgcctcttc agccggcttg cccgcccgca aggcctccag ataggccctc atctgcggcg
     2461 accgctcctt ggagaaggta aggaagtggg tcagcgccca gccttggccg tagagatcga
     2521 ggaagcgaag cttgtggggc ggcttcagaa gcaggtcggc gacgttgacc cagtcgaccg
     2581 cgatctggcc gccgcgatgc ctggccggca tgccgtagcc gatcttcccg tcggcaagga
     2641 ccttgctcga tccgatcagc tcggcgaagc cctcgacgta ccagggcgga tagatggacg
     2701 ggaaatattg aagcatgaaa tgatgggcat attcgtggtg caggacgagc gcgggggtga
     2761 agtggagatc gttcaacgtc tccctcggag tgaccgcgaa cgggccgagg atgttgctgt
     2821 cgtagaatcc cgcgatgccg ctgtcgctga gcccgagcgc ctgctcgacc tcgcgcaggg
     2881 agtcgacctc gtagatgcgc accttgaccg gctcggcctc gtcggagatg ccggtcgcca
     2941 tccgcatcag cttgtcgtag ctttccagcc gcgccgccag cttctcgata ccgcggtccc
     3001 cgctctcgct gtagaaggtg aaatgccggg tctcggcggc ccgccactcg gcctgtgccg
     3061 cctgcggagc cagcaggagc aacgtgagcc agacgaattt caacagccga tccatcgttc
     3121 atcccgcctt gaatcgtttt cgcggaccaa gcttagtgcg ctgaaggcat ttgaaaagcc
     3181 gtctcagagg aagctgtagg gatcgacgtc gaccgtcacc cgaaccccgc gcggccagct
     3241 cagcttgccg agccagtcgc ggatcacctc ctgcatgtcg acggagcggg cggcatggac
     3301 gagaaggcgc tggcggtggc gcccgcgaag catggcgagc ggcgccgggg ccgggccgaa
     3361 cacggccatg ttctccaccc gcggcgccgc ccggccgatc agcttcgccg cctccgtcgc
     3421 ctcgcccgga tcctcggacg agatgacgat cccggcgagc cggccgaaag gcggcatcgc
     3481 cgcctcgcgc ctcgactggg tctcggcggc gtagaagctt tcggaatcgc cgctcaccag
     3541 ggcctcgatc acggccgccg aaggctcgtg ggtctggacc agcacccggc ccggcttctc
     3601 gccgcgcccg gcacgcccgg cgacctgggc gatctgctgg aaggtgcgct cggccgcgcg
     3661 caggtcgccg ccctgaaggc cgaggtccgc gtcgaccact ccgaccaggg tgaggttggg
     3721 gaaatgatag cccttggtga cgagctgggt tcccacgacg atgtcgatct cgcccgcctc
     3781 catccggccc acgaactccg ccgccttggc cggcgaccag atcgtgtcgg aggtgacgac
     3841 ggcggtgcgc gcgtcgggga acagggccgc gacctcgtcg gcgatccgct cgacgcccgg
     3901 gccgacgggc acaaggctcc cttcctcgcc gcattcggga caggcctgcg gcggcggcat
     3961 catatgcccg caatggtggc aggcgaggcg gtggacgagc cggtgctcga ccatccaggc
     4021 cgtgcaattc gggcattgga agcgatgccc gcaggcgcgg cagagggtga gcggtgcgta
     4081 gccgcgccga ttgagaaaga gcaaagcctg ttcgccgcgc tccagcgtct ccttgatctc
     4141 ggagaccaga cgcggcgcga tccagcggcc gcgtggcggc ggctcggcga tcagatcgat
     4201 cgcctcgatg tccggaagct cggc
//
LOCUS       DASWNU010000505         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_228868, whole genome shotgun sequence.
ACCESSION   DASWNU010000505 DASWNU010000000
VERSION     DASWNU010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228868"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..711
                     /locus_tag="VGB57_09920"
     CDS             127..711
                     /locus_tag="VGB57_09920"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0131711.1"
                     /translation="MAVPSPSPSDKPALRKKALADRRAYAFSLDPAVRAELELKLAEI
                     VLPRLIGAKIVAAYHPLKDEISPYPILERLAAGQRALLPWFLDRDARMLWREAPAVEP
                     SPWGVLQPAASAEALAPDIVLVPLVAADRAGRRIGHGKGHYDRALSHLREGGAVTTIG
                     LAWEDQILDAPIPADPWDEPLDAIATPREWIQCR"
     gene            702..920
                     /locus_tag="VGB57_09925"
     CDS             702..920
                     /locus_tag="VGB57_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEY0131712.1"
                     /translation="MPVARPSWRKPAGIGLIVMLIVVWAALAVTAFGWIEHLPWPVHA
                     LYFLAAGIVWILPLKPLLRWMETGRWRA"
     gene            complement(957..1033)
                     /locus_tag="VGB57_09930"
     tRNA            complement(957..1033)
                     /locus_tag="VGB57_09930"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(997..999),aa:Asp,seq:gtc)
     gene            complement(1092..>1652)
                     /locus_tag="VGB57_09935"
     CDS             complement(1092..>1652)
                     /locus_tag="VGB57_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0131713.1"
                     /translation="SSFDGAMTTARAIQQMVDATSTYLGTITMVNVAMGAGVALILYF
                     IGMPTPLMWGGIVAVLNYIPYLGPIASALLLAVGGLIVFADPWYAMLPALSFIALHLV
                     EANLLTPAIIGHRLTINPLLILVALSFWAWVWGTTGALLAVPLLIILKTVLDAAGKPD
                     IAGFLFEEGTLVHEHHDELHGGASRE"
BASE COUNT          266 a          557 c          577 g          252 t
ORIGIN      
        1 cggtacgagc tttagaacat ccctgggcga taagcatcct cgggggctat ccctggtcgg
       61 gccctggtcc ggcacatatg gcccccacct gacgttacgc gtcagaggat attcgagcaa
      121 acggccatgg cggtcccgtc accctcccct tccgacaagc cggcgcttcg caagaaggcg
      181 cttgcggacc ggcgcgctta tgcgttctcg ctcgatccgg cggttcgggc cgaactcgaa
      241 ctgaagctgg cggagatcgt cctgccccgg ctgatcggcg cgaagatcgt cgcggcctat
      301 cacccgctga aggacgagat cagcccctat cccatcctcg agcgcctggc ggcaggccag
      361 cgcgccctgt tgccgtggtt cctcgaccgc gacgcacgga tgctgtggcg cgaggcgccg
      421 gcggtcgagc cgagcccctg gggcgtgctc cagccggcgg cttcggccga agcgctggcg
      481 cccgatatcg tcctggtccc gctcgtcgcc gccgaccgcg ccggccgccg catcggccac
      541 ggcaagggcc attacgaccg cgccctctcg catcttcgcg aaggcggcgc ggtcacgacc
      601 atcggcctcg cctgggagga ccagattctg gacgcgccga tcccggccga tccgtgggac
      661 gagccgctcg acgcgatcgc gaccccgagg gagtggatcc agtgccggta gcgcgcccgt
      721 cgtggcgcaa gccggccggg atcggcctaa tcgtcatgct catcgtggtc tgggcggcgc
      781 tcgccgtcac cgccttcggc tggatcgagc acctgccctg gccggtccac gcgctctatt
      841 tcctggcggc cggcatcgtc tggatactgc cgctgaaacc gctgctgcgc tggatggaga
      901 caggccgctg gcgcgcctga cgcggcgcgg gaaggggctg cgttgatggc ggaaaatggc
      961 gcgagtgacg gggctcgaac ccgcgacctc cggcgtgaca ggccggcgct ctaaccaact
     1021 gagctacacc cgcttccggg aagcgggcga gttagtggag ggtccggaat ctgtcaagca
     1081 agagcgccgg cctattcccg gctcgcaccg ccgtggagct cgtcgtgatg ctcgtggacg
     1141 agcgtcccct cctcgaacag gaagccggca atgtcgggct tgccggcggc gtccagcacg
     1201 gtcttgagga tgatcagcag cggcaccgcg agcagggccc cggtcgtccc ccagacccac
     1261 gcccagaagc tcagggcgac gaggatgagc aaggggttga tggtgaggcg gtggccgatg
     1321 atcgccgggg tcagcagatt cgcctcgacc aggtgcaggg cgatgaagct caaggcgggc
     1381 agcatcgcgt accaggggtc ggcgaagacg atcaggccgc cgaccgcgag cagcagggcc
     1441 gaggcgatcg gcccgagata ggggatgtag ttgagcaccg cgacgatgcc gccccacatc
     1501 agcggcgtcg gcatgccgat gaagtagagg atcagcgcga cgccggcgcc catcgcgacg
     1561 ttcaccattg tgatcgtgcc gaggtaggtg gaggtcgcat ccaccatctg ctggatcgca
     1621 cgggcggtgg tcatcgcccc gtcgaagctc ga
//
LOCUS       DASWNU010000506         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_436097, whole genome shotgun sequence.
ACCESSION   DASWNU010000506 DASWNU010000000
VERSION     DASWNU010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436097"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VGB57_09940"
     CDS             complement(<1..316)
                     /locus_tag="VGB57_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131714.1"
                     /translation="MAYPWRQSDWSGATPAAAASPQRLWTLAWVFVAILVSLLFVQDL
                     AATQFTPWPSIRGNAIWGRDFANVYTSGTLALEGRLGLLYDLKAYSAYQDALFDGALR
                     HHN"
     gene            589..>1749
                     /locus_tag="VGB57_09945"
     CDS             589..>1749
                     /locus_tag="VGB57_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131715.1"
                     /translation="MTSTPAERESASARRPRAWWEHPAVVLAAVLACGIPLLWPDVPP
                     LVDLPGHMGRYHVQMEIGGSPILQAFYDFKWALIGNLGVDLLVVPLAALIGLEPAVKL
                     ITIAIPMLTAAGFLWVAREVHGRVPPTAYFALPLTYSFPFLFGFLNFALSMALAFLAF
                     ALWLRLARLGRLKLRAGLFVPISILLWVCHAFGWGTLGVMAFSAELVRQVDRKRPIWK
                     AGFNTGLHCLALAPPMLLMLLWRSGAVGGETSVWFNWRLKKNWILMALRDRWEYWDYA
                     ALAVIGLVLAAGLLWWKRLEYSRNLAASAIFLAIVYVCLPRIVFGSNYADMRLAPFVI
                     AAAVIAIRYRPAWAGKAGGVFAALGLLFAGARLAGNTISFWMYDRVYDRELAA"
BASE COUNT          245 a          576 c          588 g          340 t
ORIGIN      
        1 aattgtggtg gcgaagcgcg ccgtcgaaca gcgcgtcctg ataggcgcta taggccttga
       61 ggtcgtagag caggccgagc cggccttcga gggcgagggt gccggacgtg tagacgttgg
      121 cgaagtcgcg gccccagatg gcgttgcccc tgatcgaggg ccagggggtg aattgggtcg
      181 ccgcgagatc ctggacgaaa agcaaggaga cgaggatcgc cacgaagacc caggcgagcg
      241 tccacaggcg ctgcggtgat gcggcagcgg cgggcgtcgc cccgctccag tcgctctgtc
      301 tccagggata agccattcgc gcctccttac ccgcagggct aggagaacag ggttaacgaa
      361 gggttatctt ctacttgcct ccctggcgcc gcaggcgacg gggagggggg ccaccgaagg
      421 tggtggaggg gtagctgtcg tgtattccga ggctctcgcg agaaccgact ctgtgcgccg
      481 gatcacccct ccaccatgct tcgcatggtc cccctcccca ccgcttcgcg gcagggaggc
      541 aagatataag ttgaatttcg ccgccggagg agggaggcac cggtcccgat gacgtccacg
      601 ccggccgaaa gagagagcgc cagcgcccgc cggccgcgcg cctggtggga gcatcccgcc
      661 gtcgtgctcg ccgccgtgct ggcctgcggg atccctttgc tctggccgga cgtgccgcct
      721 ctggtcgacc tgccgggtca catgggccgc taccacgtcc agatggagat cggcggctcg
      781 ccgatcctcc aggcctttta cgatttcaaa tgggctctga tcggcaatct cggggtcgat
      841 ctgctggtcg tgccgctcgc cgccctgatc gggctcgagc cggcggtgaa gctgatcacc
      901 atcgccatcc cgatgctgac ggcggccggg ttcctctggg tggcgcgcga ggtgcacggg
      961 cgggtgccgc cgaccgccta tttcgcgctt ccgctcacct acagcttccc cttcctgttc
     1021 ggcttcctca atttcgccct ttcgatggcg ctcgccttcc tcgccttcgc cttgtggctg
     1081 aggcttgcgc ggctcggtcg gctcaagctt cgcgccggcc tgttcgttcc gatctcgatc
     1141 ctgctctggg tctgccacgc ctttggctgg gggaccctgg gggtgatggc cttctcggcc
     1201 gagctggtcc gccaggtcga ccgcaaacgg ccgatctgga aggcgggatt caatacgggc
     1261 cttcactgcc ttgcgcttgc gccgcccatg cttctgatgc tgctgtggcg aagcggagcg
     1321 gtcggcggcg agacctccgt ctggttcaac tggaggctca agaagaactg gatcctgatg
     1381 gcgcttcgcg accggtggga atattgggac tatgcggcgc ttgcggtgat cggcctcgtc
     1441 ctcgccgccg gcctgctgtg gtggaagaga ctcgaatatt cgcgcaacct cgcggcctcg
     1501 gccatcttcc tcgccatcgt ctacgtctgc ctgccgcgaa tcgtcttcgg ctccaattac
     1561 gccgatatgc ggctcgcccc cttcgttatc gccgcggctg tgatcgcgat ccgctatcgc
     1621 cccgcctggg cgggcaaggc gggaggggtc ttcgcggccc tggggctgct cttcgccggc
     1681 gcccgcctcg cgggcaacac gataagcttc tggatgtacg accgggtcta tgaccgcgag
     1741 ctcgccgcc
//
LOCUS       DASWNU010000507         6507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_279429, whole genome shotgun sequence.
ACCESSION   DASWNU010000507 DASWNU010000000
VERSION     DASWNU010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279429"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="hutI"
                     /locus_tag="VGB57_09950"
     CDS             complement(<1..961)
                     /gene="hutI"
                     /locus_tag="VGB57_09950"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY0131716.1"
                     /translation="MDPAVPGAFGAIEDGAIGIQDGRIVRVGRRIDLAGYRAKAIEPQ
                     GGAWVTPGLIDCHTHLVFGGNRSAEFEQRLNGASYEEIARSGGGIVSSVKSTRFASLE
                     ELVEQARPRLRALMRGGVTTVEIKSGYGLDVPSEMKMLQAAKALGESEAVRVIPTLLA
                     LHALPPEHKESREEFVRLAIDEMLPAVAEAGLAKAVDAYCEGIGFTAEEVRSLFEAAD
                     EHGLRVKLHAEQLSNLQGAALAAEFGALSADHLEHADEAGIGAMARAGMVAVLLPGAF
                     YALRETKKPPVDLLRRHGVAMAIATDCNPGTSPVVSPTLMLSMA"
     gene            1040..1909
                     /locus_tag="VGB57_09955"
     CDS             1040..1909
                     /locus_tag="VGB57_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY0131717.1"
                     /translation="MSWKSIADLIGSEGQVAILGAPMESGSVTPGRCDLAPETLRRAL
                     RRFSTYNVETGAELFFPIRDLGDVPVQGVSPAEGFAPIRDAAAASVAEHRLTLLIGGN
                     NAVTRPAVHALGLPLAKVGLITLDAHFDLRDTAGGPMNGNPIRCLIEDGLPGSNICQI
                     GLAPFANTARMHSDAVAFGIGVYTMADCLERGMLAVLNHVLARFVHLEALAVDFDIDV
                     IERSQCPGAPGARPGGMPASMFFSAARRLAAEPRVKLVDLTEFDPSLDVSDITALTAG
                     RWVCETLAGFAGR"
     gene            complement(2077..3297)
                     /locus_tag="VGB57_09960"
     CDS             complement(2077..3297)
                     /locus_tag="VGB57_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0131718.1"
                     /translation="MTHHDVVIVGAGHAGAQAAIQLRQLGFAGSIALIGAEAEPPYER
                     PPLSKEYLAGEKEFERMLARPAAFWAERGIELVLGTTIAAVDPEARTLSAEGDVRFGY
                     GTLIWAAGGDPRRLGCEGGDLEGVHLVRRKTDVDSIRAELDGVRRVAVIGGGYIGLET
                     AAVLRKLGKEVVLLEALDRVLARVAGPEISEFYAAEHRAQGVDVRLGAAVASILGESG
                     RVAAVRLQDGATIEAEMAIVGIGVDAAVAPLLAAGADGANGVDVDAHCRTSLPDVYAI
                     GDCAAHENRFAGGLRIRLESVQNAHDQAATAAKAITGVAQPYEAVPWFWSNQYDLRLQ
                     TVGLSVGHDRTVVRGRREERSFSVLYLLAGRVIALDCVNSTRDYVQGRKLVLERRAIE
                     PERLADTTVQLKEL"
     gene            complement(3442..5103)
                     /locus_tag="VGB57_09965"
     CDS             complement(3442..5103)
                     /locus_tag="VGB57_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HEY0131719.1"
                     /translation="MAIYAGVVLAALLILGLIITRLYRRATKEIGFVRTGFGGEKVVI
                     NGGALVLPVLHETMPVNLNTVRLAVERKNVDALITLDRLRIDVKAEFYVRVRPDRESI
                     AIAAQTLGLRTMHPENLKELIEGKFVDALRSVAAGMTMNQLHEQRADFVQKVQQVSAA
                     DLAMNGLELESVSLTGLDQTSIEHFNANNAFDAEGLTKLTEQIELRRKTRNDIEQETR
                     VQIEAKNLEAARKSFEIGRDTEFARLEQEREVEVRRAAQNAEVAQQQAERQREADEAK
                     ITAKRQVDSAQIEANKVVEQARIAQTQAVEIARQEQQIAIQNKSREESQAKAEADTAR
                     AAAVAAEEKVGTARETEIAERVKRIELIDASREAERSAIGIKVQADAEKQAAADRAEA
                     MRLGAEGEAEAEKLRAEASRVRFEVEAAGQRAINEAANLLSSDQISLQTKMALLKVLP
                     EVVREAAKPMEAIDSIKIVQVEGLSGGSNAGANGGGSGGEGNLASSAVAAALRYRAQA
                     PIVDGLMKELGFDGSTLENLVAGAGNGVAAPAAAEPLVAIVQPKD"
     gene            complement(5114..5767)
                     /locus_tag="VGB57_09970"
     CDS             complement(5114..5767)
                     /locus_tag="VGB57_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HEY0131720.1"
                     /translation="MLEIIAAPANLPFSVALLVMLMIGAVEALGLGASAVHLDAHADV
                     DGGGDLLGWLGIGRVPLLMLLVVFLALFGLAGLAIQQFAGPLPLWIAVPGAVGAALPL
                     TGLGARGLARIMPGDETTAVSLDSLVGRRGTITIGTARRGSPAQARVADIHGQPHYVM
                     VEPYDDDHAIGEGETVRLDRRDGHIFIAVGRTDRLESSFEALPTPGWPVSTSSIGRD"
     gene            5906..6394
                     /locus_tag="VGB57_09975"
     CDS             5906..6394
                     /locus_tag="VGB57_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0131721.1"
                     /translation="MATEAAALTQDFKQAMRRVASTVNVITVCVGGEPMGITATAMSS
                     LSLDPPSLLICINRTASLHSPLEDVSHFCVNVLHRSQEDIAQMFADRSQQALRFASGW
                     HVDCDRPPRLADAQAAIMCRRIRHFPYGTHSIFVGEVEEVAVRADVDPLVYVDGRYGG
                     AG"
BASE COUNT          942 a         2290 c         2188 g         1087 t
ORIGIN      
        1 aggccatgct cagcatcagg gtcggcgaga cgacggggga ggtgcccggg ttgcagtcgg
       61 tcgcgatcgc catcgccacg ccgtggcggc gcagcaggtc caccggcggc ttcttcgttt
      121 cgcggagcgc atagaaggct ccggggagaa ggacggcgac catcccggcg cgggccatgg
      181 ctccgatgcc cgcctcgtcg gcatgttcga gatggtcggc cgaaagcgct ccgaactcgg
      241 ccgccagcgc cgcgccctgg agattggaaa gctgctcggc gtggagcttg accctgaggc
      301 catgctcgtc cgccgcctcg aacagcgagc gcacctcctc ggcggtgaat ccgatcccct
      361 cgcaataggc gtcgaccgcc ttcgccagcc cggcctccgc gaccgccggg agcatctcgt
      421 cgatggcgag gcggacgaac tcctcgcggc tttccttatg ctcgggcgga agcgcgtgaa
      481 gggcgagcag ggtcgggatc acccggaccg cctcgctctc ccccagcgcc ttcgccgcct
      541 ggagcatctt catttcgctc ggaacgtcga ggccgtagcc cgacttgatc tcgaccgtcg
      601 tcaccccgcc cctcatcagg gcccgaagcc gcggccgggc ctgctcgacc agctcttcga
      661 gcgaagcgaa tcgggtcgac ttgaccgagg agacgatccc gccgccggag cgggcgatct
      721 cctcgtagct cgcgccgttc agcctttgct cgaactccgc cgagcggttg cctccgaaga
      781 cgaggtgggt gtggcagtcg atcagtcccg gggtcaccca ggcgccccct tgcggttcga
      841 tcgccttcgc gcggtagccg gcgaggtcga tccgccgccc cacccggacg atccgcccgt
      901 cctggattcc gatcgccccg tcctcgatcg ccccgaaagc gcccggcacg gccgggtcca
      961 tcgtcgcgac atggcaatcg gttagcagtc ggtcccacat ggctcgcttc ttaacctttg
     1021 cccgattaag ggcaacgcca tgagctggaa atccatcgct gacttgatcg gcagcgaggg
     1081 ccaggtcgcg atccttggcg cgcccatgga atcgggttcg gtgaccccgg gccgttgcga
     1141 cctcgccccc gaaaccttgc gccgcgccct caggcgcttc tcgacctaca atgtcgagac
     1201 gggcgccgag ctgttcttcc cgattcgcga cctcggcgac gttccagtcc agggcgtcag
     1261 cccggccgaa ggcttcgcgc cgatccgcga cgccgcggcg gcttcggtcg cggagcaccg
     1321 cctgaccctc ctgatcggcg gcaacaacgc ggtgacccgc cccgcggtgc acgcgctcgg
     1381 ccttccgctc gccaaggtcg ggctgatcac gctcgacgcc catttcgacc ttcgcgatac
     1441 ggccggcggt ccgatgaacg gcaatccgat tcgctgcctg atcgaggacg gccttcccgg
     1501 ctccaacatc tgccagatcg gcctcgcgcc tttcgccaac acggcgagga tgcactccga
     1561 cgccgtggct ttcgggatcg gagtctacac gatggcggac tgcctggagc gcgggatgct
     1621 cgcggtgctc aaccacgtcc tcgcccgctt cgtccatctc gaggcgctcg ccgtcgattt
     1681 cgacatcgac gtcatcgagc ggagccaatg ccccggcgcc cccggtgccc gccccggcgg
     1741 aatgccggct tcgatgttct tctccgccgc gcgccggctg gcggccgaac ccagggtgaa
     1801 gctggtcgac ctcaccgagt tcgacccgag cctcgacgtc agcgacatca cggcgctgac
     1861 cgccggccgg tgggtgtgcg agacactggc ggggttcgcg gggcggtgat ttgcctcgcc
     1921 atgcgaagca tggggagggg gaccccgcga agcggggtgg aggggtaact cgcgcaggat
     1981 tgagaggatc atgcgcgcac ccgcaaggac ccgcgccctc tacccctcca ccaccctacg
     2041 ggtggtcccc ctccccatct gcgatgggga ggcaaatcaa agctccttca gctgaaccgt
     2101 cgtatccgcc agtcgctccg gctcgatcgc ccgccgctcc agcaccagct tcctgccctg
     2161 aacatagtcg cgggtagagt tgacgcagtc gagggcgatg acccggccgg cgagcaaata
     2221 gaggacggag aagctgcgct cctcgcggcg gccgcgtacc accgtgcggt catggccgac
     2281 ggacaggccg accgtctgaa gccgcaaatc atattgattg gaccagaacc aggggacggc
     2341 ctcgtagggc tgcgcaacgc cagtgatcgc cttcgccgcc gtcgccgcct ggtcgtgcgc
     2401 gttctgcacc gattcgaggc ggatcctgag gccgccggcg aatcggttct catgggcggc
     2461 gcaatcgccg atcgcataga cgtcgggaag gctggtccgg caatgagcgt cgacgtcgac
     2521 tccgttcgcg ccgtcggcgc ccgctgcgag cagcggcgcc acggcggcgt cgactccgat
     2581 cccgacgatc gccatctcgg cctcgatcgt ggctccgtcc tggagccgga ccgccgcgac
     2641 ccggcccgat tctccgagga tggaggcgac ggcggcgccg agccggacgt cgaccccctg
     2701 tgcccggtgc tcggccgcgt agaattccga aatctccggt cccgccacgc gcgccagcac
     2761 ccggtccagc gcctccagca gcaccacttc cttgcccagc ttgcgaagca ccgccgcagt
     2821 ctcgagcccg atatagccgc cgccgatgac cgcgacccgc ctcaccccgt ccagctcggc
     2881 gcggatcgaa tcgacgtcgg tcttgcgccg tacgagatgg actccttcca ggtcgccgcc
     2941 ctcgcagccc agccggcgcg ggtcgccccc cgccgcccag atcagggtgc cgtagccgaa
     3001 gcgcacgtcg ccctcggcgc tcagcgtcct ggcctccgga tcgacggcgg cgatcgtggt
     3061 tccgaggacg agctcgatgc cccgctccgc ccagaacgcc gcgggacggg cgagcatccg
     3121 ctcgaactcc ttttcgccgg cgagatattc cttggagagg ggcggccgct cgtagggtgg
     3181 ctcagcttcg gcgccgatca gggcgatgga gcccgcgaag ccgagctgcc gaagctggat
     3241 cgcggcctgg gcgccggcat gtccggcgcc gacgatgacg acgtcgtggt gggtcatggc
     3301 gtttcctaaa ttcctccgcg acatttgtcg gggaggggga ccgcgcgaag cgcggtggag
     3361 gggttcttcg gactcagcag gcccctccac catgcttcgc atggtccccc tccccggcca
     3421 atgccgggga ggaatttcag atcagtcctt cggctggacg attgcgacga gcggctcggc
     3481 ggccgcaggc gcggccacgc cgttgccggc gccggcgacg aggttctcga gggtcgagcc
     3541 gtcgaagccg agctccttca tcaggccgtc gacgatcgga gcctgggcgc ggtaacgaag
     3601 cgccgcggcg acggcgctgg aggcaaggtt gccttcgccg cccgagcctc cgccattggc
     3661 gcccgcattg gagccgcccg aaagcccctc cacctggacg atcttgatcg aatcgatcgc
     3721 ctccatcggc ttggccgcct cgcgcaccac ctcgggcagc accttgagga gcgccatctt
     3781 ggtctggagc gagatctggt cggaggagag caggttcgcc gcctcgttga tcgcgcgctg
     3841 gccggcggcc tcgacctcga agcgcacccg cgaggcttcg gcgcgaagct tctcggcctc
     3901 ggcctcgcct tccgcgccga ggcgcatggc ctcggcccgg tcggccgcgg cctgcttctc
     3961 ggcatcggcc tggaccttga tcccgatcgc gcttcgctcg gcttcgcggc tggcgtcgat
     4021 cagctcgatc cgcttcacgc gctccgcgat ctcggtctcg cgcgcggttc cgaccttctc
     4081 ctcggccgcg acggcggcgg cgcgggcggt gtccgcctcg gccttggcct ggctttcctc
     4141 gcggctcttg ttctggatcg cgatctgctg ctcctggcgg gcgatctcga ccgcctgggt
     4201 ctgggcgatc ctggcctgct cgacgacctt gttggcctcg atctgggcgc tgtcgacctg
     4261 ccgcttggcg gtgatcttcg cctcgtcggc ctcgcgctgc cgctcggcct gctgctgggc
     4321 gacctcggca ttctgggccg cgcgccgcac ttcgacctcg cgctcctgct ccaatcgggc
     4381 gaattcggtg tcgcggccga tctcgaagct cttgcgcgcc gcctcgaggt tcttcgcctc
     4441 gatctggacc cttgtctcct gctcgatgtc gttcctcgtc ttgcggcgaa gctcgatctg
     4501 ttcggtgagc ttggtgaggc cctcggcgtc gaaggcgttg ttggcgttga aatgctcgat
     4561 cgaggtctgg tcgaggccgg tcagcgacac cgattcgagc tcgaggccgt tcatcgccag
     4621 gtcggcggcg gagacctgct gcaccttctg gacgaaatcg gcgcgctgct cgtggagctg
     4681 gttcatcgtc atgcccgccg cgaccgagcg gagcgcgtcg acgaacttgc cttcgatcag
     4741 ctccttcaga ttctccgggt gcatggtgcg gaggcccagc gtctgggcgg caatggcgat
     4801 cgactcgcgg tcgggacgga cccggacgta gaattccgcc ttcacgtcga tgcgcaggcg
     4861 atcgagcgtg atcagggcgt cgacattctt gcgttcgacg gcgagacgga cggtgttgag
     4921 gttgaccggc atcgtctcgt ggaggaccgg gaggacgagg gcgccgccgt tgatgacgac
     4981 cttctcgccg ccgaagccgg tgcggacgaa gccgatctcc ttggtggccc ggcggtagag
     5041 gcgggtgatg atcagcccca gaatgagcag ggcggccagc acgacccccg catagatggc
     5101 catctgaacc atgtcagtct ctcccgattg aagatgttga aaccggccag cccggcgtcg
     5161 gcaacgcttc gaagctggac tcgaggcggt cggttctgcc gaccgcgatg aagatgtgcc
     5221 cgtcgcggcg gtcgagacgg actgtctcgc cctcgccgat agcgtggtcg tcgtcgtagg
     5281 gctcgaccat cacataatgg ggctggccgt ggatgtcggc gacccgcgcc tgggcgggcg
     5341 agcctctccg ggcggtgccg atcgtgatcg tgccccgcct tccgacgaga ctgtcgaggc
     5401 tgaccgcggt cgtctcgtcg cccggcatga tccgggcgag gccccgggcg ccgaggccgg
     5461 tgagcgggag cgcggccccg acggcgccgg gaaccgcgat ccacaagggc agcggtccgg
     5521 cgaattgctg gatggcgagc ccggcgaggc cgaacagggc gaggaacacg accagcagca
     5581 tcagcagcgg cacccggccg atcccgagcc agccgagcag atcgccgccg ccgtccacgt
     5641 cggcatgggc gtcgagatgg accgccgacg cgccgaggcc gagcgcctcc accgcgccga
     5701 tcatcagcat gaccagcagc gccaccgaaa atggcaggtt cgccggagcg gcgatgattt
     5761 cgagcattcc cccgtcccct tgagtcgctg ataatcgcag ttcagctatt ttcctagcaa
     5821 attccatggt ttgcccgggc ttccgaggct tggcggcggg ctaagccatt gccagcgcgg
     5881 cgcttcccgg ccatgggcgg gcgccatggc gactgaagct gcggccctca cccaggactt
     5941 caagcaggcg atgcgccgag tcgcctccac cgtgaacgtc atcaccgtct gcgtcggcgg
     6001 cgagccgatg gggatcacgg cgacggccat gtcgtcgctt tcgctcgacc ctcccagcct
     6061 gctcatctgc atcaaccgca ccgcgtcgct gcacagcccg ctggaggacg tctcgcattt
     6121 ctgcgtcaac gtgcttcacc ggagccagga ggatatcgcg caaatgttcg ccgaccggag
     6181 ccagcaggcg ttgcgcttcg cctcgggctg gcacgtcgac tgcgaccgac cgcccaggct
     6241 cgccgacgct caggcggcga tcatgtgccg gcggatccgg catttcccct acgggaccca
     6301 ctccatcttc gtcggcgagg tcgaggaggt ggcggtgagg gcggacgtgg acccgctggt
     6361 ctatgtcgac gggcggtacg gcggggcggg ctgatttgcc tccctgcggc gaagccgtgg
     6421 ggagggggac cacccgaagg gtggtggagg ggtagctgcc atagcgtcag tggatcgagc
     6481 gcgagacggc agaagcccgc gccagct
//
LOCUS       DASWNU010000508         6036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_488328, whole genome shotgun sequence.
ACCESSION   DASWNU010000508 DASWNU010000000
VERSION     DASWNU010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488328"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGB57_09980"
     CDS             <1..234
                     /locus_tag="VGB57_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131722.1"
                     /translation="ALKINAAARVLGATLNAGETAGLTLDPARHVYLVAVGGAIEVNG
                     RRAEPRDGVAVTGESKLEIKALDDAEIVLVDAR"
     gene            695..2005
                     /locus_tag="VGB57_09985"
     CDS             695..2005
                     /locus_tag="VGB57_09985"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0131723.1"
                     /translation="MNKYVVPAALAGCAALLTLPTMAAANMQSAPVRPAPPGTETSPP
                     AGPTPPPLRKAPTRPAPPTVPAGPVAPQPGKTFSASRFGDNLRNALDPVVVGWAYSIG
                     KEGAPVKADGDGFARTKVDGLRKHSADQRQNIASVSKTFTAVSTLQLLHKLGLTPQAQ
                     VSPFLKGHKLGPNMTDLTFSDVMRHCTSIDSGIFNSVDLLKYETVLSYVEKGVTKRPG
                     PGKERPCKYDNMNFALLRVLTGQMWEKAGADLMAESLGMYDQLGEMPLGDPNLIGNWN
                     AYTYIAYMQKNVLGPIGVKGALCSDKSAVQTLYYSAQGDKPGNKSFPNNDWTRACGSG
                     GWYLSANDMTNFMSHLMYTEKLLPKALRQEMFARGYGINPADTTWGTLYKKDGSVGAG
                     DGGLQACMMHLPNNYDVSVVVNSGSVPSMKLCDLMKGAYEKAWS"
     gene            2133..2681
                     /locus_tag="VGB57_09990"
     CDS             2133..2681
                     /locus_tag="VGB57_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0131724.1"
                     /translation="MRTIIAGAAVILLAACGSSGDGNGTLAEANSSGNAAAAALENKV
                     EQSAATPLQKEQALALMKQRHENYEKIGDAMKAISRELKGDSPNLGNVRAGAATIAQL
                     APQVATWFPAGTGPDVGKTEARAAIWEKPEDFAAKAKDFQEAAAAFETAARGSDLAAI
                     RSAQANLGKSCKACHDLYREEE"
     gene            2668..3342
                     /locus_tag="VGB57_09995"
     CDS             2668..3342
                     /locus_tag="VGB57_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY0131725.1"
                     /translation="MKKSDSEPGAPVWDLPVRLFHWSVVAFIGLSWWSAENGFDRVHF
                     WSGYALLFLLVFRILWGFAGSSTARFATFVSGPAAVAAYLRHGRQSQAGHSPIGALSV
                     LAMLLALLVQIASGLIQIDEDDFVEGPLSGLVGYDTALLSHDVHEASFYVLLVLIALH
                     LLAIAYYRLIRGRSLIRPMVTGRAVLPEGASPMTPAAKGRLAACAAAALILTAAVIGG
                     GGALAG"
     gene            complement(3373..3957)
                     /locus_tag="VGB57_10000"
     CDS             complement(3373..3957)
                     /locus_tag="VGB57_10000"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEY0131726.1"
                     /translation="MRFKTLFPFALFSVVAAQTAPAAETVALPRFDGIELKGGGSVVV
                     RQGAVQRVTLVEGSTSHTRIRVEPRARGGPDRLVIDACKVQCPQNYRLRIEIVTPDVS
                     AVAVDGGGRIDVATGFSPRRHVSAAVNGGGQVDLRALPALDVAAAVNGGGHLKVTATA
                     SLAAAVNGGGAILYWGDPQVSSSIRGGGAVTKGR"
     gene            complement(4211..5407)
                     /locus_tag="VGB57_10005"
     CDS             complement(4211..5407)
                     /locus_tag="VGB57_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131727.1"
                     /translation="MSLLCRLGLHRPRGIPRWNDGFYFATCERCGRDLVRTVFQRWHV
                     PKGYRVVWSDRAPASRPEVALIPEESERSNSGVPQAPPEIARGSDAESAGAGSPPGPV
                     PGRPEAPLGPSASAELPGQAPGAPAGPADERLAAGAPLPPAGPDRGRLPIQEVLAQLN
                     AEDAANRVRETPPTPQSPARYRRPAWDFMEDDPFEEDSRRGLGGSRTGEGASATSTHP
                     PEARQEEAAAPSRRAGGLPERWRALRSAVRNFSSGPAEPRPMLVIGLALGLAVAVATA
                     LAFYSPGSPQRPPAVSGRVEGAGTGERSDHFEASAPAISPSEAESTAPREGVEDPAGE
                     AVAYVAASLLSCREAPVLQARRIRNLARGQEVRVLGYDGEWASLAFRGGQCWAQAQYL
                     SPVPPL"
     gene            complement(5631..>6036)
                     /locus_tag="VGB57_10010"
     CDS             complement(5631..>6036)
                     /locus_tag="VGB57_10010"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0131728.1"
                     /translation="SIIGSGIFVDGNIEADVDLRIRGKVTGDVRCATLVLGGQGEIRG
                     NVVAERVRLSGLVEGAIETGDLSLEDTARVTGDITYSRIKMVKGAVVQGRMSHMAREP
                     AAEEEPVQAKEAPTAQKPPPPPGSGRKAVYIE"
BASE COUNT          943 a         2130 c         2030 g          933 t
ORIGIN      
        1 gccctgaaga tcaacgccgc cgcccgggtc ctcggagcga ccctgaacgc gggcgagacc
       61 gccgggctta ccctcgatcc ggcgcgccac gtctacctcg tcgccgtcgg cggagcgatc
      121 gaggtcaacg gccgccgcgc cgagccgagg gacggagtcg ccgtcaccgg cgagtcgaag
      181 ctggagatca aggcgctcga cgacgcggag atcgtcctcg tcgacgcccg ctgacacgaa
      241 taccctcccc ggcgtcggcc ggggaggatt ttgctaccga ctcgcttccc cgccgcaggg
      301 cgttccgccg gttgcggccc tcgcgccatc gaggtcatcg atcgcatcat gatcggcgga
      361 tcgccccccg cggccaaagc ggcggggccg gtgtcgcgga acagatgccg ccggacgggc
      421 tcggggatcg atccctccgg cgcgatcgga tgctcggcga gcagggcctc gtagcgggcg
      481 ttgtcggcct cgtcggcccg aaagctgtcc gaccctttcg agcagccagg ttggaatgca
      541 acattttgtg caaccttcgt gcaacggttg cgcatcgcgc cgagcgccgt tgcaactcct
      601 ccgccgggcc cgccgctcct aaaacgccgg cggggcattt ctccggcgcg accgctcaga
      661 gacgcggttc ggccggattc aaaagggtat gatcatgaac aaatatgtcg ttccggcggc
      721 gctcgccgga tgcgcggcct tgctgacgct tccgaccatg gcggccgcga acatgcagtc
      781 cgccccggtc cgtccggcac ctccgggcac ggagacgagt ccgccggccg gcccgactcc
      841 gccgcccctg agaaaggcgc cgacgagacc ggccccgccg accgtcccgg ccggacccgt
      901 cgccccccag ccgggcaaga ccttcagcgc cagccggttc ggcgacaatc tgcgcaacgc
      961 cctggatccg gtcgtcgtcg gctgggctta ttcgatcggc aaggaaggcg ccccggtgaa
     1021 ggcggacggc gacggcttcg cccgcaccaa ggtggacggc ctgcgcaagc attcggccga
     1081 ccagcgccag aatatcgcga gcgtgtcgaa gacgttcacg gccgtctcga ccctgcagct
     1141 gctccacaag ctgggcttga ccccccaggc gcaggtctcc cccttcctga agggccacaa
     1201 gctcggcccg aacatgaccg acctcacctt cagcgacgtg atgcgccact gcaccagcat
     1261 cgacagcggc atcttcaact ccgtcgacct tctgaaatat gagacggtcc tgtcctatgt
     1321 cgaaaaggga gtcacgaagc ggcccggccc cggcaaggag cggccctgca aatatgacaa
     1381 catgaacttc gccctgttgc gggtgctgac cgggcagatg tgggaaaagg ccggcgccga
     1441 cctcatggcc gaatcgctgg gcatgtatga ccagctcggc gagatgccgc tgggcgatcc
     1501 gaacctgatc ggcaactgga acgcctatac ctatatcgcc tacatgcaga agaacgtgct
     1561 cggccccatc ggggtcaagg gcgcgctgtg cagcgacaag tccgcggttc agacgctcta
     1621 ctacagcgct cagggtgaca agccgggcaa caagtccttc ccgaacaacg actggacccg
     1681 cgcctgcggc agcggcggct ggtatctgtc cgccaacgac atgaccaatt tcatgtccca
     1741 tctcatgtac accgaaaagc tactgcccaa ggcgttgcgc caggaaatgt tcgcccgggg
     1801 atatgggatc aatcccgcgg acacgacctg gggcacgctc tacaagaagg acggttcggt
     1861 cggtgccggg gacggcgggc tccaggcctg catgatgcac ctgcccaaca attacgacgt
     1921 gtcggtggtg gtcaactccg gcagcgtccc ttccatgaag ctgtgcgacc tgatgaaggg
     1981 agcctatgag aaggcctggt cgtagccgac gctgaaaaat gggggcggcc ttcccgggcc
     2041 gccccccatc ggttcgcggc ccgttcgccg ttgcgccgat gtcccgcact ttgttacgtg
     2101 gcccctgtcc catgcgaagc aggagaagtg cgttgagaac gattattgcc ggtgccgccg
     2161 tgattctcct ggccgcatgc ggctcgtccg gggacgggaa cgggacgttg gcggaggcca
     2221 attcgagcgg caacgccgcc gccgccgcgc tcgagaacaa ggtcgagcaa agcgccgcca
     2281 cgcccttgca gaaggagcag gccctggcgc tgatgaagca gcgccacgaa aattacgaga
     2341 agatcggcga tgcgatgaag gcgatctcac gcgagctgaa gggcgacagc cccaatctcg
     2401 gcaacgtgcg cgccggagcg gcgaccatcg cccagcttgc tccgcaggtc gccacctggt
     2461 tccccgcggg aaccggcccc gacgtcggca agacggaggc gcgcgccgcc atctgggaga
     2521 agcccgagga tttcgcggcc aaggcgaagg atttccagga ggccgccgcg gcattcgaga
     2581 cggcggcccg aggctccgat ctcgccgcga tccggtcggc ccaggccaac ctcggcaaga
     2641 gctgcaaggc ctgccacgat ctgtatcgtg aagaagagtg attccgagcc gggcgcgccg
     2701 gtctgggacc tgcccgtccg cctcttccac tggtcggtcg tcgcctttat cgggttaagc
     2761 tggtggtcgg ccgaaaacgg cttcgaccgg gtccatttct ggtccggcta cgctttgctg
     2821 ttcctgctcg tcttccggat cctgtggggc ttcgccggga gctcgacggc gcggttcgcg
     2881 accttcgtca gtggaccggc ggcggtcgcc gcctatctgc gccatggccg gcaaagccag
     2941 gcgggtcatt cgccgatcgg ggcgctgagc gtcctcgcca tgctactggc gctgctcgtc
     3001 cagatcgcga gcggcctgat ccagatcgac gaggacgact tcgtcgaagg gccgctttcc
     3061 ggactggtcg gctacgacac ggcattgctt tcgcacgacg tgcatgaggc aagcttctac
     3121 gtcctgctgg tgctgatcgc gctgcatctt ctcgcgatcg cttattatcg tctgatccgc
     3181 ggccgctcgc tgatccggcc gatggtgacg gggcgggcgg tgcttccgga gggcgcgtcg
     3241 ccgatgacac cggcggcaaa gggccgattg gccgcctgcg ccgccgcggc cttgatcctg
     3301 actgcagccg tgatcggagg cggcggggcc ctcgcgggct gagcccgccg cgtgcggagg
     3361 ggcgcttcgc ggctaccgcc ccttcgtcac ggcgcccccg ccgcggatcg agctcgagac
     3421 ctgcgggtcg ccccaataga ggatcgctcc gccgccattg accgcggccg cgaggcttgc
     3481 ggtcgcagtg accttcagat gcccgccgcc gttgaccgcc gcggcgacgt cgagcgccgg
     3541 aagcgctcga aggtcgacct gcccgccgcc gttcaccgcc gccgagacgt gccgccgcgg
     3601 ggaaaagccg gtcgccacgt cgatccgccc gccgccgtcg accgccacgg cggagacatc
     3661 gggcgtgacg atctcgatcc gaagccggta attttgcggg cactggacct tgcaggcgtc
     3721 gatcacgagc cggtcgggtc cgccccgcgc gcgcggctcg acccggattc gggtgtggct
     3781 cgtgctgccc tcgaccaaag tgacccgctg caccgccccc tggcggacga cgacgcttcc
     3841 gccgcccttg agctcgatgc cgtcgaaccg gggcagcgcc accgtctcag cggcgggcgc
     3901 ggtctgggcg gcgacgacac tgaagagggc gaaagggaac agggtcttga agcgcatggg
     3961 cgatctcctg gccggacaga tgcgacaagc gtagcaaggc ccgtgccagg acccgcgcag
     4021 agggagacgg cgagatcggg cgcgatcggc atccgtcggc ggacaccatt cgtccgtcag
     4081 cggacacaag tccctggctt ccccgtgcgt cgatttcgaa gtcacgctcg acaagaacgg
     4141 gggtcacgat ctggagcggc cggccccggg ctcggcctgc tcgccgcgag atatcgtttt
     4201 gtccccaacc ttaaagcggc gggaccggcg agagatactg ggcctgggcc cagcactgcc
     4261 ccccgcgaaa ggcgaggctg gcccactcgc cgtcatagcc cagcacgcgt acttcctggc
     4321 cccgcgcgag gttacggatc cggcgcgcct gcaggacagg ggcttcgcgg cagctgagca
     4381 ggctggcggc aacgtaggcg accgcctccc cggccgggtc ctccacgccc tcgcgcggcg
     4441 ccgtcgattc ggcttcgctt ggagaaatgg cgggagcgga ggcttcgaag tgatcggacc
     4501 gctctcccgt tccggcgcct tcgaccctgc cggacaccgc cggcgggcgc tgcggcgagc
     4561 ccggcgaata aaaagcgagc gcggtggcca ccgcgaccgc cagccccagc gccagcccga
     4621 tgacgagcat cggcctcggc tccgccgggc cggacgagaa attccgaacc gcggatcgaa
     4681 gcgcgcgcca ccgctcgggt aagccgccgg cccggcgcga aggcgcggcc gcttcctcct
     4741 gccgcgcttc gggcgggtgc gtcgacgtcg cggatgcgcc ctccccggtc cggctgcctc
     4801 cgaggccgcg gcgcgagtcc tcttcgaacg gatcgtcctc catgaagtcc caggccgggc
     4861 gccggtaccg ggccggactc tgcggggtcg gcggcgtctc acgcacccgg ttcgcagcgt
     4921 cctcggcgtt gagttgggcc aggacctcct ggatcggaag cctgcccctg tccggtccgg
     4981 cgggcggaag aggcgccccg gccgcaaggc gctcgtctgc cggtcccgcc ggcgcgccgg
     5041 gcgcctgccc gggaagctcc gccgacgcgg acgggccgag cggcgcttcg ggacggcccg
     5101 gaacgggtcc gggcggggag ccggcgccgg ccgattcggc atcggaccca cgggcgatct
     5161 ccggcggggc ctgcggcact cccgaattcg aacgctcgct ttcttcgggg atcagggcga
     5221 cctcgggacg gctggccggg gcgcgatcgc tccacacgac cctgtagccc ttgggtacgt
     5281 gccagcgctg aaaaaccgtc ctcaccaggt cgcggccgca ccgttcgcag gtcgcgaagt
     5341 agaagccgtc gttccagcgc gggatcccgc ggggccggtg aaggcccaac cggcacagga
     5401 ggctcacgtc cgcccgccac cgtcggaacc gcgagcgccg atcgaagccg cgcacggtcc
     5461 gccgaacccg ggggtgctct gcctcgacca ttttcccgcg cggatcatgt cttcgttcct
     5521 agcctcacct cgttgccccc ggcgccttcg acggcgccac ctggtgtccg agttccgggg
     5581 aattcgcctt gccgccgtcg aatgcacgat gcgcggcgat tcttccacgc ttactctata
     5641 tagacggcct tccggccgct ccccggtggg ggcggcggct tctgtgcagt cggcgcctct
     5701 ttcgcctgta ccggctcttc ctcggccgca ggctcgcgtg ccatatggct catccggccc
     5761 tggaccaccg cccctttcac catcttgatc cggctgtagg tgatgtcccc tgtgactcgg
     5821 gcggtgtcct cgagggagag gtctccggtt tcgatagcgc cctccaccag gccggagagc
     5881 cgcacccgtt cggcaacgac gttgccccgg atctcgccct gcccgccgag gacgagggtg
     5941 gcgcagcgga cgtcgccggt gaccttgccc cggatccgaa ggtcgacgtc cgcctcgata
     6001 ttgccgtcga cgaagatgcc gctgccgatg atggaa
//
LOCUS       DASWNU010000509         6254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_124176, whole genome shotgun sequence.
ACCESSION   DASWNU010000509 DASWNU010000000
VERSION     DASWNU010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124176"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VGB57_10015"
     CDS             <1..408
                     /locus_tag="VGB57_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131729.1"
                     /translation="VGPEKNIAKPESFAFDESGNLYIADNRDNILYLLRTDGSLHRVI
                     SDRPGFAPESLSFVRGALFITDSDNGKLFRYTPDDGLNVVAIFGGSLANIQGIAADEG
                     GALYLSVQSDLKGRKGYILKLSRKGQSPPPTSP"
     gene            535..1383
                     /locus_tag="VGB57_10020"
     CDS             535..1383
                     /locus_tag="VGB57_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0131730.1"
                     /translation="MSQDLPFDRRLRRLRRDRAALRFAEADYLHRLVADELIERLDFV
                     KRDFRRALDLGCAGGYLSRRLRERGLDVTSCDSGGAFARAAGGVQADEDRLPFADGAF
                     DLVVSAGALDSVNDLPGALILTRRALRPDGLFLGAFAGAGSLARLKRAMLAADAVGGG
                     AAARVHPQIDVRAAGDLLARAGFALPVVDAQPVDVRFGSLMDLIRDLRAMGATNILAA
                     RSRVPLGRTGLAAAIADFDTDGDKTTERFEILHLLGWSPSPDQPQPARRGSGKVSLAE
                     ALKPRT"
     gene            complement(1459..1842)
                     /locus_tag="VGB57_10025"
     CDS             complement(1459..1842)
                     /locus_tag="VGB57_10025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEY0131731.1"
                     /translation="MVAAALVDGGRRVLLQRRAPGRAMAGLWEFPGGKVEAGERPEAA
                     LARELEEELGIRVEAHSLSPAAFASADNDGRHMLLLLYLCREWQGEPRPLDATALKWV
                     RPDEMGALPMPPADEPLIPLLDRLI"
     gene            1953..2129
                     /locus_tag="VGB57_10030"
     CDS             1953..2129
                     /locus_tag="VGB57_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0131732.1"
                     /translation="MRLFFAKLVRDIKAASAVEYALICALIVLAMIAGLNAVAGRTTG
                     MWNDVSENVSDAVG"
     gene            2258..2422
                     /locus_tag="VGB57_10035"
     CDS             2258..2422
                     /locus_tag="VGB57_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0131733.1"
                     /translation="MKLIRKIFKNEEGATAIEYGLIAALIAVAAIAAMQNIGTELGET
                     FNAVADNMAV"
     gene            complement(2497..3966)
                     /locus_tag="VGB57_10040"
     CDS             complement(2497..3966)
                     /locus_tag="VGB57_10040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0131734.1"
                     /translation="MKMKALFALSVSLAAIASVGGNAPATAQSKTRSISQSTAATAAK
                     QHPQIVEEFGGEESSARGAYIKQVGARVTAQSNIAGGGSAFRITTLNSPVMNAFAVPG
                     GYLYVTRQLVGLANDEAELAFVLGHEAGHIAARHSSERKRAGIFSQILAVGLGVVTGS
                     SQLGNLAGQVTQSVVLSYSRKQELESDSLGVRYLAAAGYDPAASASLLNSLGAATSLE
                     ARAVGRDDERSTPSWARSHPLSADRVTNATRAASATGRAGTGLRNRDIFLARIDGIMV
                     DDDPRQGVIDGRTFTHPDLRLRFAVPNGYGMQNGARAVSIVGQSGQAQFGGGRFTGDL
                     GAYIGQVFQSIAGQQAQIGYGQPRGTTINGIPAAYSTARVNTQSGAVDLSVVAYRWDS
                     DTAYHFAMITPAGSGLGPFESMVGSLNRIGASEAAAIRPKIIDVVTVGPRDSVATLAA
                     RMAYNDNKEERFRVLNGLASNGALRPGQKVKIVVYGSRV"
     gene            complement(4208..5149)
                     /locus_tag="VGB57_10045"
     CDS             complement(4208..5149)
                     /locus_tag="VGB57_10045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY0131735.1"
                     /translation="MPTFLDFEKAIAELDNRIEELRETAEDSDLNIDAEIGRLDAKSE
                     RMLRETYARLTPWQKTQVARHPDRPHFKDYVAALAQDFIPLAGDRNFGEDAAIVGGLG
                     RLGGRKVMLIGHEKGDDTASRLTHNFGMAKPEGYRKAIRLMELADRFELPVVTLVDTP
                     GAFPGVQAEERGQAEAIARSTEACLALGVPMVAAIVGEGGSGGAIAIATANRVLMLEH
                     SVYAVISPEGCASILWRTAEKAADAAEAMRVTAQDLKALGVIDRIVPEPVGGAHRSPA
                     AAIAALGEAVAEELDGLAALSRGEVRADRRRKYLAIA"
     gene            complement(5164..6084)
                     /locus_tag="VGB57_10050"
     CDS             complement(5164..6084)
                     /locus_tag="VGB57_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEY0131736.1"
                     /translation="MSGDREDGQLIAAFLEMLAAEAGAALNTRLAYERDLRGATELLG
                     GRLSSAGPEALKRLSEAWMPLKRTTVARKAAALRRFYGFLHDEGLRADDPSAALPRPA
                     GGRPLPRILGHEAVGALFAELERRRAEAPGASALRLSALVELLYGSGMRATELVSLPR
                     HAVHPDRPFLILRGKGEKERLVPISDRARAAVAEYAALVPRDSLWLFPSGKSHLSRVR
                     LYQLLKELAAAAGVPPERVSPHVLRHAFATHLLEGGADLRALQMLLGHADIATTQIYT
                     HVDSRRLIDLVNSRHPLADAGRVDAKARRA"
BASE COUNT          964 a         2131 c         2176 g          983 t
ORIGIN      
        1 gtcggcccgg aaaagaacat cgccaagccg gagagcttcg ccttcgacga atcgggcaac
       61 ctctacatcg ccgacaatcg cgacaacata ctctacctgc tgaggaccga cgggagcctc
      121 caccgggtca tctccgaccg gcccggattt gcgcccgagt ccctcagctt cgtccgcggc
      181 gccctgttca tcaccgacag cgataacggc aagctgttcc gctacacgcc cgacgacggc
      241 ctgaacgtcg tcgcgatctt cggcggaagc ctcgccaaca tccagggcat cgcggctgac
      301 gagggcggcg ccctctacct ttcggttcaa agcgacctca aggggcggaa gggctatatt
      361 ctgaagcttt cgcgcaaggg tcagtcccca ccgccgacgt caccctgaac tggtttcagg
      421 gtccggttcc ccgcccgcga ggcggctcgt cgctggatgc cgaaacaagt tcagcatgac
      481 gaatggggta acgggaccct ttcgcgtcga ccgccgccgc tctaacgcga ccccgtgagc
      541 caggatcttc ccttcgaccg gcggctgcgg cggttgcggc gagatcgggc cgcgctccgc
      601 ttcgccgagg ccgactacct ccaccggctc gtcgccgacg agctgatcga gcggctcgat
      661 ttcgtcaaac gggacttccg ccgggccctc gatctgggct gcgccggcgg ctatctgagc
      721 cggcggctgc gggagcgggg gctcgacgtg acgtcctgcg attccggcgg cgccttcgcc
      781 cgggcggcgg gcggggtcca ggccgacgag gaccgcctcc ccttcgccga cggcgccttc
      841 gacctcgtcg tctccgccgg agccctcgac agcgtcaacg atctgccggg cgcactgatt
      901 ctgacccgcc gcgcgctgag gccggacgga ctgttcctcg gcgccttcgc cggcgccggg
      961 agcctggccc ggctcaagcg ggcgatgctc gcggccgacg ccgtgggcgg cggcgccgct
     1021 gcgcgggtgc acccgcagat cgacgtccgc gccgccggcg acctgctcgc ccgggcgggc
     1081 ttcgctctgc ccgtggtcga cgcccagccg gtcgacgtgc gcttcggttc gctgatggac
     1141 ctgatccgcg acctgagggc gatgggggcg accaacatcc tcgccgcccg ctcccgtgtg
     1201 ccgctgggac gcactgggct cgccgccgcg atcgccgatt tcgacacgga cggagacaag
     1261 acgacggagc ggttcgagat ccttcacctg ctcggctgga gcccctcccc cgaccagccc
     1321 cagccggcgc ggcgcggcag cggcaaggtg tcgctggccg aggcgctgaa gccgcgcact
     1381 tgagttcccc ggcgaaggcc ggggcccagc gacaaggctt gagcgactgg accccggcct
     1441 tcgccgggga gcacccccct agatcagccg gtccagaagc gggatcagag gctcgtcggc
     1501 cggcggcatc ggcagagcgc ccatttcgtc gggtcgaacc catttgagcg cggtggcgtc
     1561 gagggggcgt ggctcgcctt gccattcgcg gcacagatag aggagcagaa gcatgtggcg
     1621 gccgtcattg tcggcgctcg cgaaggcggc cggactaagc gagtgcgcct cgacacggat
     1681 tccgagctct tcctcgagct cgcgcgccaa agccgcctcc ggccgctccc ccgcctcgac
     1741 cttgccgccg ggaaattccc acagcccggc catggcccgg ccgggcgcgc gccgctgaag
     1801 gaggacccgg cgcccgccgt cgaccagcgc cgccgccacc accgtgagtg tcggaatttt
     1861 ttgcagattc gaggccagtg gaaggactcc gttaacgcaa ctcacctatc caaagcggat
     1921 tgggcgggat gccggatgtg ggaacaaggc cgatgcgact cttctttgca aagctggtcc
     1981 gcgacatcaa ggccgcgagc gcggtcgaat atgcgctgat ctgtgccctc atcgtcctgg
     2041 ccatgatcgc cggcctcaac gccgtcgccg gccgcaccac gggaatgtgg aacgacgttt
     2101 ccgaaaacgt cagcgatgcg gtcggctgat ttacgcaaaa gccgcaagtc gataaccttt
     2161 tggaaacttc cttcgaataa tcagacagtg gccgcccaaa tggacagggg gggtaggaag
     2221 tgtggacttc caaagctttg gaacaggaga ccggaacatg aaactcattc gcaaaatctt
     2281 caagaacgaa gagggtgcca ccgccatcga gtatggcctg atcgccgccc tgatcgccgt
     2341 cgccgccatt gccgccatgc agaacatcgg caccgagctc ggcgagactt tcaacgccgt
     2401 cgccgacaac atggcggtct aatcgcctga acagcgacct agtaagagga ggcggcggaa
     2461 cccaggttcc gccgcctctt tctttgcgtg ccggctctag acgcggctgc cgtagaccac
     2521 gatcttgacc ttctggcccg gcctcaaggc gccgttcgag gcgaggccgt tcaagacccg
     2581 gaagcgctcc tccttattgt cgttgtaagc catgcgcgcg gcgagcgtcg ccacggagtc
     2641 gcgcgggccg accgtcacca cgtcgatgat tttcgggcgg atcgccgccg cctcggaagc
     2701 gccgatccgg ttgagcgatc cgaccatcga ctcgaacggg ccgagcccgc ttccggccgg
     2761 ggtgatcatc gcgaaatgat aagccgtgtc cgaatcccag cgataggcga cgacgctgag
     2821 atcgactgcc cccgactggg tgttgacccg tgcggtcgaa taagcggcgg ggatgccgtt
     2881 gatcgtcgtc ccgcgcggct gcccgtagcc gatctgcgcc tgctggccgg cgatcgactg
     2941 gaagacctgg ccgatatagg cgccgagatc gccggtgaag cggccgccgc cgaactgcgc
     3001 ctgcccgctc tgcccgacga tgctgaccgc tcgagcgccg ttctgcattc cgtagccgtt
     3061 cggaacggcg aagcgaagcc tcaggtccgg atgggtgaag gtgcgcccgt cgatcacgcc
     3121 ctgcctggga tcgtcgtcga ccatgattcc gtcgatccgg gcgaggaaga tgtcgcggtt
     3181 cctgaggccc gtgccggcgc ggccggtggc gctcgcggcc ctggtcgcgt tggtgacccg
     3241 gtcggcgctg agcgggtggc ttcgagccca gctgggcgtc gaacgctcgt cgtcgcgacc
     3301 gaccgccctc gcctcgagcg aggtggcggc gcccagcgaa ttgagcaagc tggccgaggc
     3361 ggcgggatcg tagccggcag cggccaggta gcgcaccccg agactgtcgg actccagctc
     3421 ctgcttgcgc gaatagctca gcacgacgct ttgggtaacc tggccggcga gattgccgag
     3481 ctggctcgat ccggtgacga ctccgaggcc gaccgccagg atctggctga agattccggc
     3541 gcgcttgcgc tcggagctgt gccgggcggc gatatggccc gcctcgtggc cgaggacgaa
     3601 ggcgagctcc gcctcgtcat tggcgagccc gaccagctgc ctcgtgacgt agagatagcc
     3661 gcccggaacc gcgaaggcgt tcatcaccgg cgagttcagg gtggtgatcc tgaaagcgct
     3721 gccgccgccg gcgatgttgc tctgagcggt gacccgcgct ccgacctgct tgatgtaggc
     3781 gccgcgcgcg ctgctctcct cgccgccgaa ttcctcgacg atctggggat gctgcttggc
     3841 cgcggtggcg gccgtgctct gggaaatgct tcgggtcttg ctctgcgccg tggcgggcgc
     3901 gttgccgccg acggaggcga tggcggcgag cgagacggac agagcgaaca gcgccttcat
     3961 cttcatggcg gatactcctc acacaggacg attcgtcgcc gtaaggaacg ggggaaggag
     4021 ggcggggttc ctgcccgctt gcctccccgc ggcgaagccg tgggaagggg gaccatgcga
     4081 agcatggtgg aggggtgacc ttgctcagac cgagacgtgc tcgcgaggga ccgcgcaggc
     4141 ctgcgccatt acccctccac cacccttcgg gtggtccccc tccccatctg cgatggggag
     4201 gcagaaacta agcgatggcc agatacttcc ggcggcgatc cgcccgcacc tcgccgcgcg
     4261 acagcgccgc cagcccgtcc agctcctccg cgaccgcctc gcccagcgcc gcgatcgccg
     4321 cggcagggct ccggtgggcg ccgccgaccg gctcggggac gatccggtcg atgactccca
     4381 gggccttcag atcctgagcc gtcacccgca tcgcctcggc ggcgtcggcc gccttttcgg
     4441 cggtgcgcca gaggatggac gcgcagcctt cgggcgagat gaccgcatag acgctgtgct
     4501 cgagcatcag caccctgttg gcggtggcga tggcgatggc gccgcccgag cccccttccc
     4561 cgacgatcgc cgcgaccatc ggaaccccca aagcgaggca ggcctcggtc gagcgggcga
     4621 tcgcctcggc ctggccgcgc tcctcggcct ggactccggg aaaagcgccg ggcgtgtcga
     4681 ccagggtgac gactgggagt tcgaagcggt cggcgagctc catcagccgg atcgccttgc
     4741 gatagccttc gggcttggcc attccgaaat tgtgggtgag ccggctggcg gtatcgtcgc
     4801 ctttctcgtg gccgatcagc atgaccttgc ggccgcccag ccggccgagc ccgccgacga
     4861 tggcggcgtc ctcgccgaag ttgcggtcgc cggccagggg gatgaaatcc tgggccagag
     4921 cggcgacata atccttgaaa tgcggccggt ccggatggcg cgcgacctgg gtcttctgcc
     4981 agggggtgag ccgggcatag gtctcgcgca gcatccgctc cgacttcgcg tccagccggc
     5041 cgatctcggc atcgatattg aggtccgaat cctcggccgt ctcgcgcagc tcctcgatgc
     5101 ggttgtcgag ctcggcgatc gctttttcga aatcgaggaa ggtgggcatg gtcgggcgcg
     5161 gggttaggct cgccgcgcct ttgcgtcaac gcgcccggcg tcggcgaggg ggtggcggct
     5221 gttgaccagg tcgatcaggc ggcggctgtc gacatgggtg tagatctggg tcgtcgcgat
     5281 gtctgcgtgg ccgagcagca tctggagggc gcgaaggtcg gcgccgcctt ccagcaaatg
     5341 ggtggcgaag gcgtggcgga ggacgtgggg gctgacccgc tcgggcggca cccctgcggc
     5401 cgcggcgagc tccttcagaa gctgatagag ccggacccgg ctgagatggc tcttgccgga
     5461 cgggaagagc cagaggctgt cgcgcggaac cagggccgca tattcggcca ccgcggcccg
     5521 ggcgcggtcc gagatcggga cgagccgctc cttttcgccc ttgcccctga ggatgaggaa
     5581 gggcctgtcc gggtggacgg cgtggcgggg aagcgagacc agctcggtgg cgcgcattcc
     5641 cgagccgtag agcaattcga cgagcgcgct cagccgaagc gcggacgcgc ccggcgcctc
     5701 agcgcggcgg cgttcgagct cggcgaacag ggcgccgacc gcttcgtgac cgaggatcct
     5761 gggcagcggc cgcccgccgg cggggcgggg caaggcggcg gaaggatcgt cggcgcgaag
     5821 cccctcgtcg tgaaggaagc cgtagaagcg gcggagcgcg gcggccttgc gcgccaccgt
     5881 ggtccgcttg agcggcatcc aggcttcgga aaggcgcttc agggcctcgg gcccggcgct
     5941 ggaaagcctg ccgccgagca gctcggtggc gccgcgcaaa tcgcgttcgt aggccaggcg
     6001 ggtgttgagc gcggcgccgg cctccgccgc gagcatctcg aggaaggcgg cgatcagctg
     6061 cccgtcctcg cgatccccgc tcacagccgg gtcatcgcct cggccgcgat catccgcgcc
     6121 tcatattcga gccccacttc cctcagcgcc cggacgatat ggtagagatg ctcggggggc
     6181 actccgcgcc agctcgcggt ctgcatcccg attcccgcga ggagggcgac ggtgccgggc
     6241 tggtcggccc gggc
//
LOCUS       DASWNU010000510         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_71663, whole genome shotgun sequence.
ACCESSION   DASWNU010000510 DASWNU010000000
VERSION     DASWNU010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71663"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..639)
                     /locus_tag="VGB57_10055"
     CDS             complement(112..639)
                     /locus_tag="VGB57_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131737.1"
                     /translation="MQEDPPHEPPQDDQPQEPPLEDTDFEDGRDHGGPPEYDADGYTT
                     SDFHSVVQKQILNATYETLVETKMHWALVEPFLHAAREVCIGDFRRTGSLRIHGIAAE
                     GDGWAGAEEAYLSLSIADQDDGHEWLSETWWLSDLVLAGGDPARVREAARALERTVGK
                     LDAWLAAQGGPGEVG"
     gene            complement(713..1369)
                     /locus_tag="VGB57_10060"
     CDS             complement(713..1369)
                     /locus_tag="VGB57_10060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0131738.1"
                     /translation="MIEAVLFDVDGTLVDSNDLHAAAWQEAFRHFGIVLPYERIRRQI
                     GKGGDNLIPSLLPADVAERLQEEIEAFRSELFRSDYLPKARPFPGAADLLKRLRDLDA
                     KLVLATSSRQEELDFHIDKLRCRELLTGTTTRDDVEHSKPCPDIFEAALEKARPLNGG
                     LAVVVGDTPWDMKAAAKLGVPAVAVRCGGFPDGELSGAAAIYDGPPELIEAYPGWLPR
                     "
     gene            complement(1366..>1703)
                     /locus_tag="VGB57_10065"
     CDS             complement(1366..>1703)
                     /locus_tag="VGB57_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY0131739.1"
                     /translation="FGWLGATAGFGLYVSNFGNYNATYGSLGAVIVFLTWLYLSAYIV
                     LLGAELNSELEERAAAQPVRHEAPPREPTARPAATVAPALGEVAWKSAAYGLALTLLG
                     RPRRIRSAA"
BASE COUNT          260 a          576 c          598 g          269 t
ORIGIN      
        1 cgacaattgc atccagctct acgagcgcgg catgacgggc cgtaaggact aagaccgact
       61 tcgcgtctca cccgttcgcg gggaagaggc gggctcgggc gaggcggccg atcagccgac
      121 ttcgcccggg cctccttgcg cggccagcca agcgtcgagc ttcccgacgg ttcgctcgag
      181 ggcgcgtgcc gcctcgcgca cccgggccgg atcgccgccg gcaagcacga ggtccgagag
      241 ccaccaggtc tcggaaagcc attcgtggcc gtcgtcctgg tcggcgatcg acaggctgag
      301 ataggcttcc tcggcccccg cccacccatc gccctcagcc gcgatgccgt ggatccgcag
      361 gcttccggtg cgacggaaat cgccgatgca gacttcgcgc gcggcgtgga ggaacggctc
      421 gaccagcgcc cagtgcatct tggtctcgac cagcgtctcg taggtcgcgt tcaggatctg
      481 cttctggacg acgctgtgaa agtcggacgt cgtatagccg tccgcatcat attcgggcgg
      541 gcccccgtga tcgcgtccgt cctcgaaatc cgtgtcttcg aggggcggtt cctggggctg
      601 gtcgtcctgg ggcggctcgt gcggcgggtc ctcttgcatc acttcctccg ggcgggtgac
      661 gctgcgaagc agcttctccg gcggccgtcc acccggaaat cgcggcgggg gttcagcgcg
      721 gcagccagcc gggataggcc tcgatcagtt cgggcggccc gtcgtagatc gccgccgccc
      781 cgctcaattc cccatcgggg aagcccccgc aacggactgc caccgcgggc accccgagct
      841 tcgcggcggc cttcatgtcc cacggcgtat cgccgaccac gacggcgagc ccgccgttga
      901 ggggacgcgc cttttccagc gccgcttcga aaatgtcggg gcagggcttg ctgtgctcga
      961 cgtcgtcgcg cgtcgtcgtc ccggtgagca gctcgcggca acggagcttg tcgatgtgga
     1021 aatcgagttc ctcctgcctg gacgaagtcg cgaggaccag cttcgcatcc agatcgcgca
     1081 gccgcttgag caggtcggcg gcgccgggga agggccgcgc cttgggcaga tagtcgctcc
     1141 ggaacagctc gctgcggaag gcctcgatct cctcctgcag ccgctcggcg acgtccgccg
     1201 ggagcagcga cgggatgagg ttgtcgccgc ccttgccgat ttggcggcga atccgctcgt
     1261 agggaaggac gatgccgaaa tggcggaagg cttcctgcca cgccgcggca tggaggtcgt
     1321 tggagtcgac gagggtgccg tcgacgtcga agaggacggc ctcgatcatg ccgcgctcct
     1381 gatgcggcgc ggccggccga gcagggtcag ggccagcccg taggccgccg atttccaggc
     1441 aacctcgccg agcgcaggag cgacggtcgc tgccgggcgg gcggtcggct cccggggagg
     1501 cgcctcgtgc ctgacgggct gcgccgccgc gcgctcttcc agctccgaat tgagctcggc
     1561 accgagcagc acgatatagg cggacaggta cagccaggtc aggaacacga tcaccgcgcc
     1621 gagcgagccg taggtcgcgt tgtaatttcc gaaattggag acgtagaggc cgaagcccgc
     1681 cgtggcgccg agccagccga aag
//
LOCUS       DASWNU010000511         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_71735, whole genome shotgun sequence.
ACCESSION   DASWNU010000511 DASWNU010000000
VERSION     DASWNU010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71735"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VGB57_10070"
     CDS             complement(<1..1029)
                     /locus_tag="VGB57_10070"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY0131740.1"
                     /translation="MADAPARTELKVTAGPIRGSRKIHVGPLGVAMREIHLEPSSGEP
                     PLRVYDCSGPYTDPQSTIDILRGLPELRRDWIRSRGDVEEVLQREVRPEDNGQLGPDR
                     SGGVQPFPNVRKTVLRARSGANVSQMHYARQGIVTPEMEYVAVRENLGRDPLAAHALA
                     QARDGQDFGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANI
                     GNSAVASDVAAEVDKMVWSIRWGADTVMDLSTGRNIHDTREWIIRNSPVPIGTVPIYQ
                     ALEKVGGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVRLPYIPMTAKRVTGIVSRGG
                     SIMAKWCLA"
     regulatory      complement(1059..1167)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1431..1739
                     /locus_tag="VGB57_10075"
     CDS             1431..1739
                     /locus_tag="VGB57_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131741.1"
                     /translation="MHLIEILLPLADNEGKPFAPELRSAVRDELVEHFGGVTAFTRSP
                     AEGLWKEGGGEPDRDDIVILEVMADWLDRGWWRVYRTELERRFRQDEIVVRAREVELL
                     "
     gene            1793..>2195
                     /locus_tag="VGB57_10080"
     CDS             1793..>2195
                     /locus_tag="VGB57_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0131742.1"
                     /translation="MGSKPIRPAQRKVLLLGSYAPSLINFRGPLIAAMVERGHEVFAA
                     APGIDSDIAAKLVGLGATPVPVVLGRTSVNPVATWRSGRQLRETVKRIAPDVMIAYTI
                     KPVVLGAMAAQAARVPFFAAMITGMGYAFLGG"
BASE COUNT          314 a          711 c          793 g          377 t
ORIGIN      
        1 ggcgaggcac catttggcca tgatcgagcc gccgcggctg acgatgccgg tgacgcgctt
       61 cgccgtcatc gggatgtagg gcaggcgcac gccggcgtgg atggtgaaat agtcgacgcc
      121 ctgctcggcc tgctcgatca gggtgtcgcg gtagatctcc caggtgaggt cctcggccac
      181 cccgccgacc ttctccagcg cctggtagat cgggacggtg ccgatcggga cggggctgtt
      241 gcggatgatc cattcgcggg tgtcgtggat gttgcggccg gtggagaggt ccatcaccgt
      301 gtcggcgccc cagcggatcg accagaccat cttgtcgacc tcggcggcga cgtcggaggc
      361 gacggcgctg ttgccgatat tggcgttgat cttgacgagg aagttgcggc cgatcgccat
      421 cggctcgctc tcgggatggt tgatgttgga ggggatgatg gcccggcccc tcgccacctc
      481 gtcgcggacg aattcggggg tgacgtaatc ggggatggag gctccgaaat cctggccgtc
      541 cctcgcctgc gcgagggcat gcgcggctaa tgggtcgcgg cccagattct cgcgcaccgc
      601 gacatattcc atctcgggcg tgacgatccc ctgccgggca tagtgcatct ggctgacgtt
      661 cgcgcccgag cgcgctcgaa gcaccgtctt gcggacgttg ggaaagggct ggactccgcc
      721 cgaacggtcg gggccgagct ggccgttatc ctccggcctg acctcgcgct ggagcacctc
      781 ctcgacgtcg ccgcggctcc ggatccagtc gcgcctcagc tcggggaggc cgcgcaggat
      841 gtcgatggtg gattgcggat cggtataggg gccggagcaa tcgtagaccc tgagcggcgg
      901 ctccccggag ctcggctcga ggtggatctc gcgcatcgcc acgccgagcg gtccgacgtg
      961 gatcttgcgg gagccgcgga tcgggccggc ggtgactttg agttcggtgc gggcaggtgc
     1021 gtcggccatg agtcgctctc caaatgcgga cggagagcgg gactgccgcg ctccctccct
     1081 acgccggtgt cagccggatc aggttcgacg ggtcggaggc tgctgcctcc ctctcaaccg
     1141 cgctgagcgg tcccccgggg atgggctcgt gtaggccgtt cctcggcggg gcgaaagcgg
     1201 gaatccggca cctcgtcatc ccggcggaag ccgggacctc ggggaggccc gccgcgactc
     1261 ttcccgcccg gggtcccggc tttcgccggg atgacggaag gcgctccagc cttcgattcg
     1321 ccggctggtc tcgcgctcct cgaaatcgac gctgtcgctc gaagcctaaa cccggttagg
     1381 gccgatgccg cccggatgaa ccaccggcgg gctgcggcgt tcgctccatc gtgcatctca
     1441 tcgaaatcct cctgcccctt gccgacaatg agggaaagcc gttcgcgccg gagctgcgct
     1501 cggccgtgcg cgacgagctg gtcgagcatt tcggcggggt caccgccttc acccgaagcc
     1561 ccgccgaggg gctgtggaag gagggcggcg gcgaaccgga ccgcgacgac atcgtcatcc
     1621 tcgaagtgat ggcggactgg ctcgaccgcg gctggtggcg cgtctaccgg accgagctcg
     1681 agcgccgctt ccgccaggac gagatcgtcg tgagggcgcg cgaggtcgaa ttgctctaga
     1741 ggcgtccgct cccttaatgg gggcttcgaa acgcggggca tgaggaaatc aaatgggctc
     1801 gaagccgatc cgcccggcgc aacgcaaggt gcttctgctc ggctcctacg cgccgtcgct
     1861 gatcaacttc cgggggcctc tgatcgcggc gatggtcgag cgcgggcacg aagtgttcgc
     1921 cgcggcgccc ggcatcgact ccgacattgc cgccaagctg gtcgggcttg gagcgactcc
     1981 ggtgccggtg gttctggggc ggaccagcgt caatccggtc gcgacctggc ggagcggtcg
     2041 gcagctacgc gagacggtga agcggatcgc gcccgacgtc atgatcgcct acacgatcaa
     2101 gccggtcgtg ctcggcgcga tggcggcgca agcggcgcgg gtccccttct tcgccgcgat
     2161 gatcaccggg atgggctacg ccttcctcgg cgggt
//
LOCUS       DASWNU010000512         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_20146, whole genome shotgun sequence.
ACCESSION   DASWNU010000512 DASWNU010000000
VERSION     DASWNU010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20146"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VGB57_10085"
     CDS             complement(<1..430)
                     /locus_tag="VGB57_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEY0131743.1"
                     /translation="MASPHFIVFANEKGGTGKSTTAVHSAVALAAAGRRVAALDLDTR
                     QRTLGRYLDNRAESVKRLGIDLVMPAYETFDPDKGEAIEAQMERISADVEVVVVDTPG
                     RDDPHARAAMIRADTLVTPINDSFVDLDLIGQVDAETYRVR"
     gene            503..1348
                     /gene="panC"
                     /locus_tag="VGB57_10090"
     CDS             503..1348
                     /gene="panC"
                     /locus_tag="VGB57_10090"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY0131744.1"
                     /translation="MQTVRDIETLREAVAALRAQGGTIAFVPTMGALHAGHMALVAEG
                     RRRASHVVASIFVNPTQFAAGEDLSTYPRREATDAKMLEDEGCAILWSPDVGTMYPEG
                     FATDVRAGSLGDGLDGAARPGHFDGVATVVARLFEQVHPDIALFGEKDYQQLAVIRQM
                     VRDRALDVDIVGVPTQRDADGLALSSRNTYLTDEERQSARALPRALGEAAASIQRGGD
                     VAQALATARERLAKAGFDPIDYVELCDSETLAPVTAVERPARLLAAARIGRTRLIDNL
                     AVEPQ"
     gene            complement(1477..>1707)
                     /locus_tag="VGB57_10095"
     CDS             complement(1477..>1707)
                     /locus_tag="VGB57_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY0131745.1"
                     /translation="PMGGIASTPVINRPEVAILAVNKVEEKPVVVDGELEIRKRMNLS
                     LSCDHRVVDGWDAANFMQDLKKLVENPLKLLS"
BASE COUNT          248 a          576 c          605 g          278 t
ORIGIN      
        1 ggcggacccg gtaggtctcg gcgtcgacct gcccgatcag gtcgaggtcg acgaagctgt
       61 cgttgatagg cgtgaccagc gtgtcggccc ggatcatcgc cgcccgggca tgaggatcgt
      121 cgcggccggg ggtgtcgacg acgaccacct cgacgtccgc cgagatgcgc tccatctggg
      181 cctcgatggc ctcgccttta tccgggtcga aggtctcgta ggccggcatg accagatcga
      241 tgcccagccg cttcacgctc tccgcccggt tgtcgagata gcggcccagg gtgcgctggc
      301 gggtgtcgag gtcgagggcc gccacccggc gcccggcagc cgccagcgcg accgcgctgt
      361 gcaccgccgt ggtcgacttg ccggtgccgc ctttctcatt ggcgaatacg atgaaatgcg
      421 gcgacgccat ctgctaccct tgattctgta agccgccccc cgcaagggcg tcgcccaagc
      481 tgatatcgga ggcgggaacg gggtgcaaac ggtccgtgac atcgaaacgc tgcgcgaggc
      541 ggtggcggcc ttgcgggcgc aagggggcac gatcgccttc gttccgacca tgggcgcgct
      601 ccatgccggc cacatggccc tggtcgcgga ggggcggcgg cgggcgagcc atgtcgtcgc
      661 ctcgatcttc gtcaacccga cccagttcgc ggccggcgag gatctctcca cctatccccg
      721 gcgcgaggcg accgatgcca agatgctgga ggatgaaggc tgcgcgatcc tgtggtcgcc
      781 ggacgtcggg accatgtatc cggagggttt cgccaccgac gttcgcgccg gaagcctcgg
      841 cgacggcctc gacggcgccg cccgccccgg ccatttcgac ggggtcgcga cggtggtggc
      901 gcggctgttc gagcaggtcc acccggacat cgcgctgttc ggcgagaagg attaccagca
      961 gctcgccgtt atccgccaga tggtgcggga ccgggctctc gacgtcgaca tcgtcggagt
     1021 cccgacccag cgcgacgccg acggcctcgc cctctcgtcc cgcaacacct atctgaccga
     1081 cgaggagcgg cagtcggcgc gggccctgcc gcgggcgctg ggcgaggcgg cggcttcgat
     1141 ccagcgcggc ggcgacgtgg cccaggccct ggcgacggcg cgggagcggc tcgcgaaagc
     1201 cggcttcgat ccgatcgact atgtcgagct gtgcgactcg gagactctgg cgccggtgac
     1261 tgcggtcgag cggccggcgc ggctgctggc cgcggcgagg atcgggcgga cccggctgat
     1321 cgacaatctg gcggtcgagc ctcaataatt cctccccggc atttgccggg gagggggacc
     1381 aggcgaagcc tggtggaggg gttcttcaga cgccgtggaa cccctccacc acccttcggg
     1441 tggtccccct cccccggaaa tccgggggag gattttctac gaaagcagct tcagcgggtt
     1501 ttcgaccagc ttcttcaggt cctgcatgaa attggccgcg tcccagccgt cgaccacgcg
     1561 gtggtcgcag gagagggaca ggttcattcg cttgcggatc tcgagctcgc cgtcgaccac
     1621 gaccggcttc tcctccacct tgttgacggc gaggatggcg acctcggggc ggttgatcac
     1681 cggggtcgag gcgatcccgc ccatcgg
//
LOCUS       DASWNU010000513         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_174839, whole genome shotgun sequence.
ACCESSION   DASWNU010000513 DASWNU010000000
VERSION     DASWNU010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174839"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..936)
                     /locus_tag="VGB57_10100"
     CDS             complement(22..936)
                     /locus_tag="VGB57_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEY0131746.1"
                     /translation="MFMHNKKLQYTVRVAEPNPTLGTFMLEQFGGPDGELAAAMRYFT
                     QGLAEEDPGRKDMLLDIATEELSHLEIIGSIVAMLNKGAKGKMSEGTMSEAELYMDLA
                     KSGASTPTQQIVFGGGPSLTNSAGVPFSGAYVDSRGEPTVDLRSNIAAEARAKIVYER
                     LINITDDPGIKDALTFLMTREIAHQPSLEKALYAIEENFPSGKLPGHPDYSSLYVNAS
                     QGDGDLDGPWNSGPEWDRLDEVASAIPLGGGDGLATVEGLSSREKKDLKATAVRTISD
                     PTRNPTTGTDLGAGPGAGRTKELDADPE"
     gene            complement(1052..1375)
                     /locus_tag="VGB57_10105"
     CDS             complement(1052..1375)
                     /locus_tag="VGB57_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3140 domain-containing protein"
                     /protein_id="HEY0131747.1"
                     /translation="MSLDRPAIAADFAAAVNMDADEIETWLETAESKRVGFKRDGGES
                     VGHASGRRIVEILRHGPREDADYAHMRKVVGFVRRHRAQEPANMVTSRWRYSLMNWGH
                     DPLKA"
     gene            complement(1382..2659)
                     /locus_tag="VGB57_10110"
     CDS             complement(1382..2659)
                     /locus_tag="VGB57_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0131748.1"
                     /translation="MRKTLLAAFLLCAAAAPASSQSVVVTADRMIDVLAGRVVEEPVV
                     VITNGRIASVVGRGGARPVIPAGAKRIDLPGKTLLPGLIDMHVHLDSNPRYGGYSGLQ
                     FTDMFWVAQGVRNARNMLDAGFTTVRNVGSDNYADVGYKQAIDEGLMAGPRIVPAAHA
                     LSATGGHCDQTYLPPSFAARGKAVGDGAQELRTRVREQRKYGAEVIKVCATGGVFSRN
                     TEPGQQQLSEEELRAIADEAHQWGLKVAAHAHGSAGIKAAIRAGIDTIEHASLIDAEG
                     IRLAKERGTWLAMDIYNTEYTQAEGAKNGVLEDNLRKDREIAQLQRDNFRAAHRAGVK
                     MVFATDAGVMPHGSAGGQFRWMVQYGMTPMEAIQSATRNAAQALGRERDVGAIAVGRF
                     ADMIAVDGDPLADVRQLEDVDVVVKGGVVVKGE"
     gene            complement(2745..3380)
                     /locus_tag="VGB57_10115"
     CDS             complement(2745..3380)
                     /locus_tag="VGB57_10115"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0131749.1"
                     /translation="MNPVSVRNLFVAGSVLLLAFTPVCSAYAEVVGPAAAAAEPAPAA
                     VETATDLAPGEFVWQPERSPAGEVEMVVSIPLQRAYVYRGGTLIGVTTVSTGKPGHRT
                     PTGRFDILQKRPRHFSNLYNNAPMPFMQRLTWGGIALHAGQIPGRPASHGCVRLPLEF
                     ARNLFSVTRVGGSVHIVDQSPAPREALAMIRGNGAYAGMGGPLEEVPAGTD"
BASE COUNT          526 a         1271 c         1311 g          666 t
ORIGIN      
        1 cgccggcgcg cccaccggct cctactcggg atcggcgtcc agctccttgg tccggccggc
       61 gcccgggccg gcgccgaggt cggtgcccgt ggtcgggttg cgggtcgggt cggagatggt
      121 gcgaacggcc gtggccttga ggtctttctt ctcccggctg ctgaggcctt cgaccgtggc
      181 gaggccgtcg ccgccgccga gcgggatggc gctcgcgacc tcgtcgagcc ggtcccattc
      241 gggtccgctg ttccaggggc cgtcgaggtc gccgtccccc tgcgacgcgt tgacgtagag
      301 gctcgaataa tcggggtggc ccggcagctt gcccgagggg aaattctcct cgatcgcata
      361 gagcgccttc tcgagcgatg gctggtgggc gatctcgcgg gtcatcaaaa aggtgagcgc
      421 gtccttgatc ccgggatcgt cggtgatgtt gatcagccgc tcgtagacga tcttggcccg
      481 ggcctccgcg gcgatgttcg agcgcaggtc gaccgtgggc tcgccgcgcg aatcgacata
      541 ggcgccgctg aacggaaccc cggccgaatt ggtcagcgag ggcccgccgc cgaaaacgat
      601 ctgctgcgtc ggagtgctcg cgccggactt ggcgagatcc atgtagagct cggcttcgct
      661 catcgtgcct tcggacatct tgcccttggc gcccttgttg agcatcgcga cgatcgagcc
      721 gatgatctcg aggtggctca gttcctcggt ggcgatgtcg agcagcatgt ccttgcggcc
      781 gggatcctcc tcggccagcc cctgggtgaa gtagcgcatc gcggcggcaa gctcaccgtc
      841 cgggcccccg aactgttcga gcatgaaggt gccgagggtc gggttgggct cggcgactcg
      901 aaccgtatat tgcagcttct tgttatgcat gaacattgcg gaggctccgg gggaatggtc
      961 cgtgccgaac ccccgggcgc ggacgcccgt tccccgaatc aggaaagata gtcgttacgc
     1021 taccgctact tagcttgatc tatggtcatg atcacgcctt cagcggatcg tggccccagt
     1081 tcatcaggct gtagcgccag cgcgacgtga ccatgttcgc cggctcctgc gcccggtggc
     1141 ggcggacgaa gccgacgacc ttgcgcatgt gggcataatc ggcgtcttcg cgggggccgt
     1201 ggcgaaggat ctcgacgatt cgccggccgg aagcatggcc gaccgactcg ccgccgtcgc
     1261 gcttgaagcc gacgcgcttg ctctccgccg tctccagcca ggtctcgatc tcgtcggcgt
     1321 ccatgttcac ggcggcggcg aagtcggcgg cgatcgccgg gcggtcgagg ctcacgggag
     1381 tctattcgcc tttgaccacc acgccgccct tgacgacgac gtcgacgtcc tcgagctggc
     1441 gaacgtccgc gagcgggtcc ccgtccaccg cgatcatgtc cgcgaagcgc ccgaccgcga
     1501 tcgctccgac gtcccgctcc cgcccgagcg cctgcgcggc gttgcgggtc gcggactgga
     1561 tcgcctccat cggagtcatc ccgtactgga ccatccagcg gaattggccg ccggcgcttc
     1621 cgtgcggcat cactccggcg tcggtggcga acaccatctt caccccggcc cggtgcgccg
     1681 cccggaaatt gtcgcgctgg agctgggcga tctcgcggtc cttcctgaga ttgtcctcga
     1741 ggactccgtt cttcgccccc tccgcctggg tatattcggt gttgtagatg tccatcgcca
     1801 gccaggtgcc gcgctccttc gcgagccgga tcccttcggc gtcgatcagt gaagcatgct
     1861 cgatcgtgtc gatgccggcg cggatcgcgg ccttgatccc ggccgagcca tgggcgtggg
     1921 cggcgacctt caggccccat tgatgcgcct cgtcggcgat cgcccgcaac tcctcctcgg
     1981 aaagctgctg ctggcccggc tcggtgttgc gcgagaagac tcccccggtc gcgcagacct
     2041 tgatcacctc cgcgccatat ttgcgctgct cgcggacgcg ggttcggagc tcctgggcgc
     2101 cgtcgccgac cgccttgccc ctggcggcga aggagggcgg caaataggtc tggtcgcaat
     2161 ggccgccggt ggcggacagg gcatgggcgg ccggcacgat ccgcggcccg gccatcagcc
     2221 cttcgtcgat cgcctgcttg tagccgacgt cggcataatt gtcggagccg acgttgcgca
     2281 ccgtcgtgaa gccggcgtcg agcatgttgc gggcgtttcg aacgccctgc gcgacccaga
     2341 acatgtcggt gaactggagc cccgaatagc cgccgtagcg cgggttcgaa tcgaggtgga
     2401 cgtgcatgtc gatcagcccg ggcagaaggg tcttgcccgg aaggtcgatc ctcttcgcgc
     2461 cggccgggat gaccgggcga gcgccgcccc gcccgaccac cgaggcgatg cgcccgttcg
     2521 tgatcaccac caccggctcc tcgaccaccc ggccggcaag cacgtcgatc atccggtcgg
     2581 cggtgacgac gacgctttgc gaggaagcgg gcgcggcggc ggcgcagagg aggaaggcgg
     2641 cgagcagggt ctttcgcatc ggcgggcggg ctccgggctt tgttgcgccc ggtgactagc
     2701 cgcccggccg gtgacagtca ctagggactg tcccccgtct ccgctcagtc ggtgccggcc
     2761 ggcacctcct ccagcggccc gcccatcccg gcataggcgc cgttgccgcg gatcatggcc
     2821 agcgcttcgc ggggcgccgg cgactggtcg acgatgtgca ccgagccgcc gacccgggtc
     2881 accgagaaca ggttcctggc gaattccaga ggcagccgga cgcagccgtg cgaggccggg
     2941 cggccgggga tctggccggc gtggagcgcg atcccgcccc aggtcagccg ctgcatgaac
     3001 ggcatcggcg cattgttgta gaggtttgag aagtgcctgg ggcgcttctg caggatgtcg
     3061 aacctgccgg tcggagtgcg atgacccggc ttgccggtcg agacggtggt cactccgatg
     3121 agggtgccgc cgcgatagac ataggcgcgc tgcaggggga tgctgaccac catctccact
     3181 tcccccgccg gcgagcgctc cggctgccag acgaactcgc ccggggcgag gtccgtcgcc
     3241 gtctcgaccg cggcgggcgc cggctcggcg gcggcggcgg cgggtccgac gacctcggca
     3301 taagccgagc agaccggggt gaaggccagc aggaggacgc tgccggcgac gaagagattg
     3361 cggacgctta ctgggttcat ggctcgactt gcgggtgttt atcccgctcc tttcctgcgg
     3421 ccggaatgac cgtggcgatg cgtctaggcc cgggtcatga cgccgctgtg ccgcggaaaa
     3481 ggctttgttg tttcgattgt tgcagtgcaa aaatttggcg gtttgggcgg aatccgccgg
     3541 atgtgcgtcg cctcttgccg gatcgttccg cccccacatc cgtttcacgc ccgggtgcgg
     3601 tttcgcccat gtgctttgcg taggaggccg atcatgccgg caagattttc cgcgatcctt
     3661 ctcgtcaccc tcgccgcggc ggcgcccgcc gccgctcagg attacgaccg gcgaagccgg
     3721 gcggagcgcg tcgccgaaga catcgcccgc cagatcgagg ccacggcggg cgcg
//
LOCUS       DASWNU010000514         5431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_460647, whole genome shotgun sequence.
ACCESSION   DASWNU010000514 DASWNU010000000
VERSION     DASWNU010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460647"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGB57_10120"
     CDS             <1..457
                     /locus_tag="VGB57_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
                     /protein_id="HEY0131750.1"
                     /translation="ADTRLGDVFIWGLFYPKFVHPIVWGEPGDAARIGKTLAEDLPRE
                     FDYLERELPEAGWLFGDDIGLAEIAIATFFRNGAHVGVEPDRARWPRVAGFVGRALAH
                     PCFASLLPFEQVQLGTTPAGRRQALLAAGAPLTAETMAAREPRRGVMRL"
     gene            547..981
                     /locus_tag="VGB57_10125"
     CDS             547..981
                     /locus_tag="VGB57_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131751.1"
                     /translation="MDGSTRTPSHLWVVGAAMTLVNGTSCFEYLMTQTRNVRYSANFT
                     DPQRVYFESFPLWMDAVWAFGVWGGLAGSLLLLARSRHAVTAFAVSLAGLAISTVYQY
                     ALADPPEDIWIGAMMGGTVILWAVAVGLLLYALRMRERGVLR"
     gene            complement(978..1526)
                     /locus_tag="VGB57_10130"
     CDS             complement(978..1526)
                     /locus_tag="VGB57_10130"
                     /inference="COORDINATES: protein motif:HMM:NF004861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0131752.1"
                     /translation="MRYPLDRIGITGASGCGVTTLGAALAVRLGAVHIDMDDHFWVAT
                     DPPYRVKRELPERLARIRSEQARTGRWVVSGTLDGWAEPVSRDAQLIVFLLVPTQVRL
                     ERLRRRERERFGDALLPGGTMHENHREFLEWAAHYDDGTLPGRNLPRHEEWLAGLSTP
                     VLRLDGTRRTDELVRAVLESEA"
     gene            1661..2920
                     /locus_tag="VGB57_10135"
     CDS             1661..2920
                     /locus_tag="VGB57_10135"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF005536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY0131753.1"
                     /translation="MSADGKSGHIRLTSHPGSRGPVRFPLTWGAATPAERGPVIATVN
                     ARSDRNAIGAHGGSYSIYRALAISSGAMDPSAKPDLHDTSPVCEIGPHKQWGDPGKIV
                     SLDPWGHRVAQDFGELIEEGIDIRPSIAITRARLTLHEIQGAIAAGRLEVDGDIVHEA
                     GDVSVTKIAVDPVWWLPGVAERFGVAEGSLRRCLFEQTGGMYPELVTRPDLSVFLPPI
                     GGMTLYIFGDPAALGDTSKTLTCRVHDECNGSDVFGSDICTCRPYLTHGIEEATRQAQ
                     AGGTGLIVYNRKEGRALGEVTKFLVYNARKRQPGGDQAATYFERTECVAGVQDARFQQ
                     LMPDVIHWLGITRIDRFVSMSNMKYDALAAGGVEIGERVPIPDGLVPPDASVEMEAKK
                     AAGYYTPEGAPSEEHLRSVVGRDLGKF"
     gene            2947..4113
                     /locus_tag="VGB57_10140"
     CDS             2947..4113
                     /locus_tag="VGB57_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HEY0131754.1"
                     /translation="MRRLLTAAAVRERAHEMLDLALEGGVDGWAVDLGRLDSAAELTA
                     RVTREAYPDLAIPFHARWRHFVAGEPRLPGTGGAARARAAFDLVILSVLLDAGTGPGW
                     RFADPVSGETFTRSEGLAVASQRLVETGALGTSGAALEALDGEILKHGFQVSDSNPLA
                     GLDGRTALLRRLGGQVLARPDLFATAGEPRPGGLYDLLAARAAAGRLPAPAILELLLE
                     ALGPIWADRLVLDGVPLGDCWRHPAIRRDDPTDGLVPLHKLSQWLAYSLIEPLEAAGI
                     EVVDVDGLTGLAEYRNGGLLVDTGVLNLTDPESASRPHQISDPLIVAWRSMTVALLDR
                     LAPLVRERLGVREADFPLARMLEGGSWAAGRRIAAELRPGGGPPFTIISDGTVF"
     gene            4116..4745
                     /gene="upp"
                     /locus_tag="VGB57_10145"
     CDS             4116..4745
                     /gene="upp"
                     /locus_tag="VGB57_10145"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY0131755.1"
                     /translation="MDGLTIVSHPLVQHKLTILRDKSSSILLFRTVVREIATLLCYEV
                     TRDLPTDMVRIETPIAITDAPAIAGKKLVFAPILRAGLAMAEGMLDLVPSARVAHIGL
                     YREPVSLECVEYYFKTPDDVGERLVIIVDPMMATGHTAIAAIDRLKEAGAVDMRFVCL
                     LCSRPGVEAVRAAHPDVPIWTAAMDEKLNDHGYIVPGLGDAGDRTYGTR"
     gene            complement(4765..5220)
                     /locus_tag="VGB57_10150"
     CDS             complement(4765..5220)
                     /locus_tag="VGB57_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0131756.1"
                     /translation="MLLALAPPGSAAPSAPAPVTLPEAAREAAAAVDAFHAALRRGDA
                     EAALALVAEDALVFEDGRVERTKAEYALHHAAADAAFSKAVPSKRLSRTGHSTADLAL
                     IASESRTKGRFRGQDIDRIMVETMVLRRESGGAWKIVHIHWSSAEPSTE"
BASE COUNT          803 a         1921 c         1836 g          871 t
ORIGIN      
        1 tgccgacacc cgcctcggcg acgtcttcat ctgggggctg ttctacccga agttcgtcca
       61 ccccatcgtc tggggcgaac ccggcgacgc ggcgcggatc gggaagacgc tggcggagga
      121 cctgcctcgc gaattcgatt atctcgagcg cgagctcccc gaagcaggct ggctgttcgg
      181 cgacgatatc ggcctcgccg aaatcgcgat cgccaccttc ttccggaacg gcgcccatgt
      241 cggcgttgag cccgatcgcg cccgctggcc gcgggtcgcg ggcttcgtcg ggcgcgctct
      301 cgcccatccc tgtttcgctt cgctgctgcc gttcgagcag gtccagctcg gcaccacccc
      361 cgccggccgc cgccaggcac tgctcgccgc cggcgctccc ctgacggcag agacgatggc
      421 ggcgagggag ccccggagag gggtgatgag gctgtagccg gagccggtct tcagccgact
      481 tgccaggaat ggcgaagggt gcgagcctgt cgcgcagcga cctgcaacgg ctcgggggaa
      541 gcgaaaatgg acggttcgac caggacacct tcgcatctgt gggtcgtggg cgcggcgatg
      601 accctggtga acggcaccag ctgcttcgag tatctgatga cccagacccg caacgttcga
      661 tattcggcca atttcaccga ccctcagcgc gtctatttcg aaagcttccc gctctggatg
      721 gacgcggtgt gggcgttcgg cgtgtggggt gggctggccg gttcgctgct gctcctggcg
      781 cgcagccgcc acgccgtcac cgccttcgcc gtatctctgg cggggctcgc gatcagcacc
      841 gtctatcaat atgcgctggc ggatccgccc gaggacatct ggatcggtgc gatgatggga
      901 gggaccgtaa tactctgggc ggtagcggtc gggcttctcc tctatgcgct gcggatgcgc
      961 gagcggggcg tcctgcgcta agcctcgctc tcgaggacag ctcggaccag ttcgtcggtc
     1021 cgccgggtgc cgtcgagccg aagcaccggc gtggacaggc cggcgagcca ttcttcatgc
     1081 ctcggcaggt ttcgcccggg cagggtgccg tcgtcataat gagcggccca ttcgaggaat
     1141 tcgcggtggt tctcgtgcat cgtcccgccg gggaggagag cgtcgccgaa ccgctcccgc
     1201 tcgcgccggc ggagccgctc cagtcgcacc tgcgtcggca ccaggaggaa gacgatcaac
     1261 tgggcgtcgc ggctcacggg ctcggcccag ccgtcgagcg tgccagacac gacccagcgc
     1321 cccgtgcgcg cctgctcgga gcggatccgg gcgaggcgct cgggcaactc gcgcttcacc
     1381 cggtaaggcg gatcggtggc aacccagaaa tggtcgtcca tgtcgatatg gacggcgccg
     1441 aggcgcaccg cgagcgccgc gccgagcgtg gtcacgccgc agccggaggc gccggtgatt
     1501 ccgatccggt cgagcgggta gcgcattgcg ccccttttgc atccgcgcgc cgggctttcc
     1561 agtgtcgctt ggcgtccctc gcctgcaggg aggggcctag ccaccccgcc cccgctggcg
     1621 tagcgtactg agcaagaaca agaagcgaga ggaaatggcg atgagcgcgg acgggaagag
     1681 cggccacatc aggctgacgt cgcatcccgg gtcgcgcggg ccggttcggt ttccgctgac
     1741 gtggggcgcg gccaccccgg ccgagcgggg gccggtcatc gccaccgtca acgcgcgctc
     1801 ggatcgcaac gccatcgggg cgcatggcgg ctcctattcg atctaccggg ccctggccat
     1861 ctcgtcgggg gcgatggacc ccagtgcgaa gccggacctg cacgacactt cgccggtctg
     1921 cgagatcggc ccccacaagc aatggggcga tcccggcaag atcgtctcgc tcgatccctg
     1981 gggacaccgc gtcgcccagg atttcggcga gctgatcgag gaaggaatcg acatccggcc
     2041 gtcgatcgcg atcaccaggg cgcggctgac ccttcacgaa atccagggcg cgatcgcggc
     2101 cggccggctc gaggtcgacg gcgacatcgt ccacgaggcg ggcgacgtct cggtgaccaa
     2161 gatcgcggtc gatccggtct ggtggcttcc gggcgtggcc gagcgcttcg gagtggcgga
     2221 gggaagcctc aggcgctgcc tgttcgagca gaccggcgga atgtatcccg agctggtcac
     2281 ccgcccggac ctgtcggtct tccttccgcc gatcggcgga atgaccctct acatcttcgg
     2341 cgatcccgcg gctctcggcg atacctcgaa gaccctgacc tgccgggttc atgacgaatg
     2401 caacggctcc gacgtgttcg gctccgacat ctgcacctgc cgcccctacc tcacccacgg
     2461 catcgaggag gcgacccgcc aggcccaggc gggcggcacc ggcctcatcg tctacaaccg
     2521 caaggaaggc cgggccttgg gcgaagtgac caagttcctg gtctacaacg cccgcaagcg
     2581 gcagccgggc ggcgaccagg cggcgaccta tttcgagcgc accgaatgcg tcgccggggt
     2641 gcaggacgcg cgcttccagc agctcatgcc cgacgtgatc cactggctcg ggatcacccg
     2701 catcgaccgc ttcgtgtcga tgtccaacat gaagtacgac gccctcgcgg ccggcggggt
     2761 ggagatcggc gagcgggtgc cgatccccga cggtcttgtg ccgcccgacg ccagcgtcga
     2821 gatggaggcg aagaaggccg ccggctatta cacgcccgag ggggcgccga gcgaggagca
     2881 tctccgctcc gtggtcggac gggacctggg gaagttctag agcggatgca ggattccgcc
     2941 gaaccgttgc gccgcctgct gacggcggcc gccgtgcgcg agcgggccca cgagatgctc
     3001 gacctcgcgt tggagggcgg tgtcgatgga tgggccgtcg atcttggccg actcgattct
     3061 gcggccgagc ttaccgctcg ggtgacgaga gaggcctatc ccgacctcgc catccccttc
     3121 cacgcccgct ggcggcattt tgtcgccggg gagccgcgcc tgcccggcac cggcggggcg
     3181 gcgcgggcgc gggccgcgtt cgacctcgtc atcctctccg tcctgctcga cgcgggaacg
     3241 gggccgggct ggcgcttcgc cgatcccgtg agcggcgaga cgttcacccg gtccgagggt
     3301 ctcgcggtcg ccagtcaaag gttggtcgaa accggagccc tcggcaccag cggcgcggcg
     3361 ctggaggcgc tcgacggtga gatattgaag cacggatttc aggtgagcga ttccaacccg
     3421 ctcgccggcc tcgacggccg caccgccctc ctcaggcgcc tcggcggcca ggttctggcg
     3481 cggccggacc tgttcgccac ggcgggcgag ccacgccccg gcggtctcta cgacctgttg
     3541 gccgcccggg ccgccgcggg caggctgccg gcgccggcga tcctcgaact gctgctcgaa
     3601 gcgctcggcc cgatctgggc ggaccggctg gtgctcgacg gggtgccgct cggcgattgc
     3661 tggcgtcatc ccgcgatccg ccgcgacgat ccgaccgacg ggctcgtgcc gctccacaaa
     3721 ttgtcgcaat ggctcgccta ttcgctgatc gagccgctcg aggcggcggg catcgaagtg
     3781 gtcgacgtgg acggcctcac cgggctcgcc gaatatcgca acggcgggct tctcgtcgac
     3841 accggcgtcc tgaacctcac cgatcccgaa tccgcttcga ggccccacca gatttcggat
     3901 ccgctgatcg tcgcctggcg ctcgatgacc gtggctctcc tcgaccggct ggcgccgctg
     3961 gttcgggaac gactcggtgt gcgcgaagcc gacttccccc tcgcccggat gctcgagggc
     4021 ggcagctggg cggcgggccg gcgtatcgcg gccgagctcc gccccggcgg cggcccacct
     4081 ttcacaatca tcagcgacgg aacggtattc tagacatgga cggactgacg atcgtctcgc
     4141 accccctggt gcagcacaag ctcaccatcc tccgcgacaa aagcagctcg atcctgcttt
     4201 tccgcaccgt cgtgcgcgag atcgcgaccc tgctctgcta cgaagtgacc cgagacctgc
     4261 cgacggacat ggtccggatc gagactccta tcgccattac cgacgccccg gccatcgccg
     4321 gcaagaagct ggtcttcgcg ccgatccttc gcgccggcct cgccatggcc gagggcatgc
     4381 tcgacctcgt cccctcggcc cgcgtcgccc atatcggcct ctatcgcgag ccggtgtcgc
     4441 tcgaatgcgt cgaatattat ttcaagaccc cggacgacgt cggcgagcgc ctcgtcatca
     4501 tcgtcgatcc gatgatggcg accggccaca ccgccatcgc cgccatcgac cgcctcaagg
     4561 aggccggagc ggtcgacatg cgcttcgtct gcctgctctg ctctaggccc ggggtcgagg
     4621 ccgtccgggc ggcgcacccc gacgtcccga tctggaccgc ggcgatggac gagaagctca
     4681 acgaccacgg ctacatcgtc cccggcctcg gcgacgccgg cgaccggacc tacgggacgc
     4741 gctgagcccg ctccagggcc gccgtcactc cgtcgacggc tccgccgacg accagtggat
     4801 atggacgatc ttccacgccc cgccggactc gcggcgaaga accatcgtct cgaccatgat
     4861 tcggtcgatg tcctggcccc tgaagcggcc cttggtccgg ctttcgctgg cgatcagggc
     4921 gaggtcggcc gtcgaatggc cggtgcggct cagccgcttc gacggcaccg ccttcgagaa
     4981 cgccgcgtcg gcggcggcat ggtgaagggc atattcggcc ttggttcgct cgactcgccc
     5041 atcctcgaag acgagcgcgt cctcggcgac cagcgccagc gccgcctcgg cgtcgccccg
     5101 gcgaagcgcg gcatggaagg cgtcgacggc ggcagccgcc tcgcgcgccg cctcggggag
     5161 agttaccggc gccggcgcgg acggcgcggc ggagccgggc ggcgcgagag ccaggagcat
     5221 gacgaccgcc gctcctcgta atgccaaacc caccatgacc tgccctttcc cgaagcggct
     5281 gttctaggcg gacaattgtt tgtttttcaa cacccttcgc tgcgccagcc ggagcgccct
     5341 cgagcgctct ccccccgccg cggagcggcg atccgatttc ctttctacgc tgtgccttcg
     5401 tgccttctct cgaaacgctt gcggcacttc g
//
LOCUS       DASWNU010000515         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_174987, whole genome shotgun sequence.
ACCESSION   DASWNU010000515 DASWNU010000000
VERSION     DASWNU010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174987"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1349)
                     /locus_tag="VGB57_10155"
     CDS             complement(30..1349)
                     /locus_tag="VGB57_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY0131757.1"
                     /translation="MRKFVLVGAALLLLLALILFVAGCERATLPVGAGIGPAPTLPKP
                     AKSLLPTVNIAEATGWRGGEPAPAPGLKVTAFAEGLDHPRWLHVLPNGDVLVAETNAP
                     ARPAKTGPRARIENFMMKRAGAAGPSANRITLLRDSDGDGIAETRSVFLSGLNSPFGM
                     ALVGGDLYVANTDAVIRFPYRTGETRIAAAGSRLTGLPAGPRNHHWTKTIVAGPDGAR
                     LYASVGSNSNVAEHGMAEEEGRAAIWEIDRATGARRLFATGLRNAVGLAIEPRSGRLW
                     AAVNERDELGSDLVPDYMTSVRRGGFYGWPYSYFGRHVDKRVKPGRPDLVASAIVPDY
                     ALGPHTASLGLAFYQGGLLGRRFAGGAFVGQHGSWNRRPASGYKVIFIPFAGGRPAGP
                     PVDVLTGFLGPDGKARGRPAGVAVDKAGALLVADDVGNAIWRVTPAS"
     gene            1436..>1820
                     /locus_tag="VGB57_10160"
     CDS             1436..>1820
                     /locus_tag="VGB57_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0131758.1"
                     /translation="MIPRRRLGHGGPEVSAIGLGCMGMSEFYGTGDEAESIATIHQAI
                     ELGVDFLDTADMYGVGRNEELVGRAIADRRERVFLATKFGNVRGEDGSFKGVCGRPDY
                     VRSACEASLRRLGVETIDLYYQHRVD"
BASE COUNT          290 a          619 c          656 g          255 t
ORIGIN      
        1 gatgggccat gaaatgcgcg ccgacaagct caagacgccg gcgtcacccg ccagatcgca
       61 ttgccgacgt cgtcggcgac gagcaacgcc cccgctttgt cgaccgcgac tcccgccgga
      121 cggccgcggg ccttgccgtc cgggccgagg aagcccgtca ggacgtccac cgggggcccg
      181 gccgggcggc cgcccgcgaa ggggatgaag atcaccttgt agccgctggc cggccttcgg
      241 ttccaggagc cgtgctgccc gacgaaggcc ccgccggcga agcgccgacc gagcaggccg
      301 ccctggtaga aggcgaggcc gagcgaagcc gtgtgcgggc cgagcgcgta atcggggacg
      361 atcgcggagg cgacgagatc cggccgcccc ggcttcaccc tcttgtccac gtggcggccg
      421 aaataggaat agggccagcc gtagaagccg ccccgcctga cggaggtcat atagtcgggg
      481 acgaggtcgc tgccgagctc gtcgcgctcg ttcaccgccg cccacagcct tccgctccgg
      541 ggctcgatcg cgagaccgac ggcgttgcgc agcccggtcg cgaacaggcg cctggcgccg
      601 gtggcgcggt cgatctccca gatcgccgcc cgtccttcct cctcggccat gccgtgctcg
      661 gcgacgttgc tgttagagcc gacgctcgca tagagacgcg ccccgtcggg gccggcgacg
      721 atggtcttgg tccagtgatg attgcgcggt ccggccggga gtccggtgag ccggcttccc
      781 gcggcggcga tccttgtctc ccccgtccgg taggggaagc gaatcacggc gtcggtattg
      841 gcgacgtaga gatcgcctcc gaccagggcc attccgaagg gcgagttgag gccggagagg
      901 aagacgctcc gggtctcggc gattccgtcg ccgtccgagt cgcgcagaag tgtgatccgg
      961 ttcgcgctcg gcccggcggc gccggcccgc ttcatcatga aattctcgat cctggcccga
     1021 ggccccgtct tggccggccg cgccggagca ttggtctcgg cgaccagcac gtcgccattg
     1081 ggcaggacgt ggagccagcg cgggtgatcg agcccctcgg cgaaggcggt aaccttcagg
     1141 cccggcgcgg gcgcgggttc gccgcctcgc cagccggtcg cctcggcgat gttgacggta
     1201 ggaaggagcg acttggcggg cttcggcagc gtcggcgccg ggccgatacc ggcgccgacc
     1261 ggtaaggtcg cccgctcgca gccggcgacg aagaggatca gcgccagaag gaggaggagc
     1321 gcggcgccga ccaggacgaa cttgcgcatg gacatgaatg cttcctccgg ccgcggttga
     1381 ggcggcgcgt cgaggagcct aaggcgacct ccgcaccgga acaaggagag gcagcatgat
     1441 cccgcgcagg cggcttgggc acggaggccc cgaagtgtcg gcgatcggcc tcggctgcat
     1501 gggcatgtca gaattctacg gcaccggcga cgaggcggaa tccatcgcca cgatccacca
     1561 ggcgatcgaa ctcggagtcg acttcctgga cacggccgac atgtatggag tcgggcgcaa
     1621 cgaagagctg gtcggacggg ccatcgccga ccgccgcgag cgggtgttcc tggctaccaa
     1681 attcggcaac gttcgcggcg aggacggaag cttcaagggc gtgtgcggac gtcccgacta
     1741 tgtccgctcc gcctgcgagg cgagcctcag gcgcctcggc gtcgagacca tcgacctcta
     1801 ctaccagcac cgcgtggacc
//
LOCUS       DASWNU010000516         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_175004, whole genome shotgun sequence.
ACCESSION   DASWNU010000516 DASWNU010000000
VERSION     DASWNU010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175004"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1128)
                     /locus_tag="VGB57_10165"
     CDS             complement(52..1128)
                     /locus_tag="VGB57_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY0131759.1"
                     /translation="MPRPEVRHGRGAPENRTPTRFNLPVREADGDWLDLREEIDDGPP
                     PLRTTVTVEKPKSIIAWNRSPDIGFDRSINSYRGCEHGCIYCFARPTHAFHDLSPGLD
                     FETKLFAKPDAAKILREELARPGYVVRSMAIGTNTDPYQPIEAEWRITRSVLEVLAET
                     KHPVTITTKSDRVMRDIDILAPMAAQGLASVALSVTSLDPAVSRTLEPRAPSGRKRLA
                     AVKALSDAGIPTVVAIAPVIPGITDHEVEHIVEAAAEAGASGAFFLPVRLPHEVAPLF
                     RAWLDEHFPERADKVMNIIRSMRGGRDNDPNWFTRMSGSGPWAELMRVRFEIAARKHG
                     LNRDKRKLRTDLFVPPQGAQLRLL"
     gene            complement(1336..>2191)
                     /locus_tag="VGB57_10170"
     CDS             complement(1336..>2191)
                     /locus_tag="VGB57_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0131760.1"
                     /translation="LGISGPGATGLHNYRDSEWRSIAGKPNVRAALALAEIGEVDLAD
                     QFLRHQARIGGAGDHSALLHLAADLNLASAQYWLAHNAPRGASVNFAARYPRPDWQPT
                     RGWRVDPSLAFAHALQESNFRTSVVSPAGAMGLMQVRPGTAGDIARARGEPFDRQMLA
                     SPAANIEFGQSYIETLRDHGATGGLLPKVIAAYNAGPVPVAEWNARRFDKGDPLLYIE
                     SLPYWETRGYVPIVLRNYWIYEEEAGKPGPSRAALAQGLWPKFPGMAGASAVRLKADE
                     TKLAYGAE"
BASE COUNT          331 a          724 c          767 g          369 t
ORIGIN      
        1 ctccaccagg cttcgcctgg tccccctccc catctgcgat ggggaggcaa gctacagcag
       61 ccgcaattgc gccccctgcg gcgggacgaa caaatccgtc ctcagcttcc gcttgtcccg
      121 gttcagcccg tgcttcctcg ccgcgatctc gaagcgcacg cgcatcagct ccgcccaggg
      181 gccggagccg ctcatccggg tgaaccagtt ggggtcgttg tccctgccgc cgcgcatcga
      241 gcggatgatg ttcatcacct tgtcggcccg ctccgggaaa tgctcgtcga gccaggcccg
      301 gaacaggggc gcgacctcgt gaggcagccg aaccggcagg aagaaggcgc cgctcgcccc
      361 ggcctcggcc gccgcctcga cgatatgctc gacctcatgg tcggtgatcc cgggaatgac
      421 cggcgcgatc gccacgacgg tagggatccc ggcgtcgctc agcgccttca ccgccgccag
      481 ccgcttccgg cccgagggcg cccgaggttc gagggttcgg gaaacggccg ggtcgagcga
      541 ggtcaccgac agcgcgacgg aggcgaggcc ttgggccgcc atcggcgcga gaatgtcgat
      601 gtcgcgcatc acccggtccg atttggtcgt gatcgtcacc ggatgcttcg tctcggcgag
      661 cacctcgagc accgagcggg tgatccgcca ctcggcttct atcggctggt agggatcggt
      721 gttggtcccg atcgccatcg agcggacgac gtagccgggc ctggccagct cctcgcgcag
      781 gatcttcgcg gcgtcgggct tggcgaacaa tttcgtctcg aagtcgaggc cgggcgacag
      841 atcgtggaag gcgtgggtcg gccgggcgaa gcaatagatg cagccatgct cgcagccccg
      901 gtacgaattg atcgagcggt cgaagccgat gtcgggcgag cgattccagg cgatgatcga
      961 tttgggcttc tcgacggtga ccgtggtcct gagcggcggc gggccgtcgt ctatctcctc
     1021 acgcaagtcg agccagtcgc catccgcctc ccgcaccggc aggttgaagc gcgtcggcgt
     1081 ccggttctcg ggcgcgccgc ggccgtggcg gacttcgggg cggggcatgg gttccgtata
     1141 agtgaacagc tagaacggag caagaacatc tttgcctccc cattgccaga tggggagggg
     1201 gaccatgcga agcgtggtgg aggggtaact tgactcagtg gtggcagatt cgcgcgtgaa
     1261 ctgctggtct cagcgccttc tacccctcca ccaccctgcg ggtggtcccc ctccccatct
     1321 tcgatgggga ggcaatcact cggcgccgta agccagcttc gtctcgtccg ccttgagccg
     1381 aaccgcgctc gctccggcca tgcccgggaa tttcggccac aggccctgcg ccagcgccgc
     1441 ccggctcggg cccggcttcc ccgcttcctc ctcgtagatc cagtaattgc gcagaacgat
     1501 cggcacgtag ccgcgcgttt cccaataagg cagcgactcg atgtagagga gcgggtcgcc
     1561 cttgtcgaag cggcgggcgt tccattcggc gaccgggacc ggcccggcat tgtaggcggc
     1621 gatcaccttg ggcaggaggc ctccggtcgc gccatgatcg cgcagggtct cgatatagct
     1681 ttgcccgaat tcgatattgg ccgccgggct ggccagcatc tggcggtcga aaggctcgcc
     1741 ccgcgccctg gcgatgtcgc cggcggtgcc cggtcgaacc tgcatcagcc ccatcgctcc
     1801 ggccgggctg accaccgaag tccggaagtt cgattcctgg agcgcatggg cgaaggccag
     1861 cgacggatcg acccgccagc cgcgggtcgg ctgccagtcg gggcgtggat aacgggccgc
     1921 gaagttgacg ctggcgcctc tcggggcgtt gtgcgcgagc caatattggg ccgaggcgag
     1981 gttgaggtcg gcggcgaggt ggagaagggc gctgtggtcg ccggcgcccc cgatccgcgc
     2041 ctggtggcga aggaactggt cggcgaggtc gacctcgccg atttcggcca gggcgagggc
     2101 ggcgcggacg ttgggcttgc cggcgatcga gcgccattcg gagtcgcggt aattgtgaag
     2161 gccggtcgcg ccggggccgc ttatgcccaa g
//
LOCUS       DASWNU010000517         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_305352, whole genome shotgun sequence.
ACCESSION   DASWNU010000517 DASWNU010000000
VERSION     DASWNU010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305352"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGB57_10175"
     CDS             <1..675
                     /locus_tag="VGB57_10175"
                     /inference="COORDINATES: protein motif:HMM:NF023780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0131761.1"
                     /translation="SVHGITRAKVGGAEDAAQSAEEREAYSEWASRLSYAAVHRLWQL
                     LLKGLAEVHGAPMPLEAAEMALLRVIHASELPDPGALLEKLASGEAMPARAASAPAGA
                     TAMPATFRDMVEALEARGRRHLAQQLHDYARPIAYAPPELVLSLKGPIATRDLAAALK
                     EVTGSSWQVRTEDEGGEPTLQEQELAAKRAEEQSVLETPLVKAALEAFPGAELARYNL
                     DEQRSA"
     gene            677..1000
                     /locus_tag="VGB57_10180"
     CDS             677..1000
                     /locus_tag="VGB57_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY0131762.1"
                     /translation="MPDLDELLKMAQNAQAGLMKAQEGLDQIEVEGLSGGGLVKIRAT
                     AKGRIISVDLDESLLVPSEKQMVEDLVAAAINDARVKADQAAQTEMSKMTAGLQLPPG
                     FKMPF"
     gene            complement(1348..2052)
                     /gene="mtgA"
                     /locus_tag="VGB57_10185"
                     /pseudo
     CDS             complement(1348..2052)
                     /gene="mtgA"
                     /locus_tag="VGB57_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
BASE COUNT          342 a          764 c          716 g          314 t
ORIGIN      
        1 agcgtccacg gcatcacccg ggccaaggtc ggcggcgccg aggacgcggc ccagtcggcc
       61 gaggagcgcg aggcctattc ggaatgggcg agccggctct cctacgcggc cgtccaccgg
      121 ctctggcaat tgctgctcaa gggcctggcc gaggtccatg gcgcgccgat gccgctcgag
      181 gccgccgaga tggcgcttct tcgagtgatc cacgcctccg agcttccgga tcccggcgca
      241 ttgctcgaga agctggcgag cggcgaggcg atgccggcgc gcgccgcctc ggcgccggcc
      301 ggcgcgacgg cgatgccggc gaccttccgg gacatggtcg aggcgctcga agccaggggc
      361 cgccgccatc tcgcccagca gctccacgat tacgcccggc cgatcgccta tgcgccgccc
      421 gagctcgtcc tcagcctgaa gggtccgatc gccacgcgcg acctcgcggc ggctctgaag
      481 gaggtgaccg gcagttcctg gcaggtgagg accgaggatg aaggcggcga gcccacgctc
      541 caggagcagg agctggcggc gaagcgggcg gaagagcagt cggtgcttga aacccctttg
      601 gtcaaagcgg cgttggaggc cttcccgggg gccgagctcg cccgctacaa tctcgacgaa
      661 caacggagcg cctgaaatgc ccgatctcga tgaactgttg aagatggccc agaacgccca
      721 ggcgggcctc atgaaggcgc aggaagggct cgaccagatc gaggtcgaag gcctgtcggg
      781 cggcggcctg gtcaagatcc gagccacggc caagggccgg atcatctcgg tcgatctcga
      841 cgagagcctc ctcgtgccga gcgagaagca gatggtcgag gatctcgtcg ccgccgcgat
      901 caacgacgcg agggtgaagg ccgatcaggc cgcccagacc gagatgtcga agatgaccgc
      961 cggcctgcag ctcccgcccg gcttcaagat gccgttttga cgctcacgca atgagcgtca
     1021 ccccggcgaa agccggggcc tcggggagac ccgcccaacc cgtcaccccg gcgaaagccg
     1081 gggcctcggg gagacccggc gagacttctt gtcctgagat cccggctttc gccgggatga
     1141 cggctactga gcattcccct cggccaccgg ctcaagctcc gcctcaggct ccgccgcggg
     1201 ctccgccacc gccggagccg ccggcaactg ctcatactcc tccccgggca gtgccgcggg
     1261 cggcggcgcc gccttcgcct tcggcgccgc cttgcgcttg accggaaccg gcggctcgcg
     1321 ctccggcgcc accgtctcgc cctcgtagac gcagcggtcc agcccttcgc gtgccaccac
     1381 cccgatccgg gcgacgatgg cgttgccgta gcgccgggtg aagcccttgg gcgcggtcgc
     1441 tccgcgcttc ttgggcaaag gcaggacggc cgcgatccgc gccgcctcga cccggctcat
     1501 cgcgctggcg tcatggttga agtagcgcag ggcgccggca ttgactccgt aggtggcgat
     1561 cccggtctcg gcgacgttga gatagacctc catgacccgc ctcttgcccc acatgttctc
     1621 gatgagcagg gtgaaccagg cttcgagccc cttgcggaaa tagcccccgc cttgccacag
     1681 gaaggcgttc ttcgccgtct gctggctgat cgtcgagccg ccgcggatga ccgagccgcc
     1741 cttcgcgttg cgccgcatcg ccgccgcgat cgcgtcctga tcgaagcccc agtgcgagca
     1801 gaacttgctg tcctcggcgg cgatcgcggc gcgcacggcg tcccggtcga tcctgtcgag
     1861 gctcatccag tccctcgaga tatttcgccc gccgagccgg tcgccgagca tggtgaaggt
     1921 gattggcggg ttcacgaagc gatagaggag gacccagagc aggctgagca ggacgaacag
     1981 gaacacgccc gaaaccgccc agatcacgat ccgcccgaac cagccccgct tcttcgctct
     2041 cacccgtgcc atcgcgaccc gttagccggg cctgcggcgc tcgtcacccg ccgtcatgct
     2101 gaacttgttt cagcatccag cgacgagccg cggtgc
//
LOCUS       DASWNU010000518         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_436622, whole genome shotgun sequence.
ACCESSION   DASWNU010000518 DASWNU010000000
VERSION     DASWNU010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436622"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..934
                     /locus_tag="VGB57_10190"
     CDS             89..934
                     /locus_tag="VGB57_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0131763.1"
                     /translation="MPELRLVQVDAFADRPFTGNPAAVVPLEAWLPDELLQAIAEENN
                     LSETAFTVPATDGEADYELRWFTPTVEVALCGHATLASGHVLIGERERLRFRTRQAGI
                     LIVGRDGAGYRLDLPQTRCSLATEEQMRDVVARDGASIVDIITHDGGSDSAPPELYLS
                     YEGSEGVAILLFADEAAVRALAPDMKRLAEIDYMVIATAPGGGEHDVVSRVFVPAWGV
                     DEDPVTGSAHGALTPFWSERLGRDTFTAFQASRRGGRVECRRARDRAILGGRCVTVIE
                     GKLSF"
     gene            complement(984..1481)
                     /locus_tag="VGB57_10195"
     CDS             complement(984..1481)
                     /locus_tag="VGB57_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0131764.1"
                     /translation="MPVRSAGILLYRRDPDGVRLLLIHPGGPFWRGRDEGAWMIPKGL
                     VEPGEDPLAAALREYEEELGAKPVGEPRPLCEVRQAGGKWVRAFALEGDFDPAELRSN
                     EFTIEYPPKSGSFRAFPEVDEARWFTLAEGRAKILKSQAPILDALEADLDNSSSRRKP
                     GPRGD"
     gene            complement(1481..>2267)
                     /locus_tag="VGB57_10200"
     CDS             complement(1481..>2267)
                     /locus_tag="VGB57_10200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0131765.1"
                     /translation="VSMVLGTALIWYFILPVRGSFLLEGSWAALTLGAGLLTEAVIVL
                     ALGLYQREIRAGQLERTRRINFLGHALKEMDHRTKNNFQIVTSLLTLQANRSSSEEVK
                     AALNEAAERLKAVAAVYDALAPSSQGLAMVRLQDQLQEICDQIRRGIMPDGIGLSTEL
                     EAILVPHESAVAIGIIVNELVTNACKHAFGDGGGSIRVKAWRDGAAAVIEVADDGKGF
                     VAGAAKPGLGSRLVTAFLQRLKARSDVQSSPEGTIHRIRVPLA"
BASE COUNT          352 a          813 c          744 g          358 t
ORIGIN      
        1 atccacatcg atcccgaagg ccaggtcgac cagcccggca atgcgcttgc cgaaaccgac
       61 ctcaccaacg ctccggagga atagcccgat gcctgaattg cgcctggttc aggtcgacgc
      121 cttcgccgac cgtcccttca ccggcaatcc cgcggcggtc gtgccgctcg aggcctggct
      181 ccccgacgag ctccttcagg cgatcgcgga agagaataat ctttccgaaa ccgccttcac
      241 ggtgccggcc acggacggcg aggcggatta cgagctgcgc tggttcactc cgacggtgga
      301 agtggccttg tgcggccatg ccacgctggc gagcgggcac gtcctgatcg gcgagcggga
      361 acggctccgc ttccgcaccc gccaggccgg gatcctcatc gtcggccgcg acggcgccgg
      421 ctaccggctc gatctgccgc agacccgctg cagcctcgcc acagaggagc agatgcgcga
      481 cgtcgttgcc cgggacggcg cgagcatcgt cgacatcatc acccatgacg gcggcagcga
      541 ctccgccccg cccgagcttt acctcagtta cgaagggtcc gagggcgtcg ccatcctgct
      601 cttcgccgac gaagcggctg tccgcgcgct tgcgcccgac atgaagcggc tcgccgagat
      661 cgactatatg gtcatcgcca ccgccccagg cggcggcgag catgacgtgg tcagccgggt
      721 cttcgttccc gcctggggag tcgacgagga cccggtcacc ggctcagccc atggcgccct
      781 cacccccttc tggtcggagc gcctcggccg cgacaccttc accgccttcc aggcgagccg
      841 ccgcggcggc cgggtggaat gccggcgggc gagggaccgg gccatattgg gaggcaggtg
      901 cgtaacggtg atcgagggaa agctgagctt ctgaattccc gtcaccccgg cggaagccgg
      961 ggcctcgggg agacccgccg agactaatcg ccccgaggtc ccggctttcg ccgggatgac
     1021 gaattgtcca gatccgcttc cagcgcgtcc aggatcggcg cctggctctt caatatcttc
     1081 gcccgcccct cggcgagcgt gaaccagcgc gcctcgtcca cctccgggaa ggcccggaag
     1141 cttccgctct tcggaggata ttcgatggtg aattcgttgc tcctgagctc cgccgggtcg
     1201 aaatcgccct ccagcgcgaa ggcccggacc catttcccac ccgcctgtcg gacctcgcac
     1261 aacggccgcg gctcgccgac gggcttcgcg cccagttcct cctcatattc gcgcaacgcg
     1321 gcggcgagcg ggtcctcgcc cggctccacc agccccttcg ggatcatcca ggcgccctcg
     1381 tccctgccgc gccagaaggg gcctcccgga tggatgagca gcagccgcac gccgtccgga
     1441 tcgcggcggt agagcaggat tccggcgctt cggacgggca ctaggcgagc ggcacccgga
     1501 tccggtggat ggtgccctcc ggcgaggact ggacgtcgct cctcgccttc agccgctgga
     1561 ggaaggcggt caccaggcgg cttccgaggc cgggcttggc cgccccggcc acgaagccct
     1621 tgccgtcgtc cgccacttcg atgacggcgg cggcgccgtc gcgccacgcc ttcacccgga
     1681 tcgagccgcc gccgtcgccg aaggcgtgct tgcaggcgtt ggtgacgagc tcgttgacga
     1741 tgatcccgat cgcaaccgcg ctctcgtgcg gcaccaggat cgcctcgagc tcggtcgaca
     1801 ggccgatacc gtcgggcatg atgccgcgcc ttatctggtc gcatatctcc tgaagctggt
     1861 cctggaggcg gaccatggcc aggccctggc tgctcggcgc gagcgcgtcg tagacggcgg
     1921 ccacggcctt cagccgttcg gccgcctcgt tgagcgccgc cttgacctcc tcgcttgacg
     1981 agcgattcgc ctgaagcgtc agcaggctgg tgacgatctg gaaattgttc ttggtgcggt
     2041 ggtccatttc cttgagcgca tggccgagga agttgatccg acgcgtccgc tcgagctgcc
     2101 cggcgcggat ctcacgctgg tagaggccga gcgcgagcac gatgaccgcc tcggtgagga
     2161 gaccggcgcc cagggtaagg gcggcccacg aaccctcgag aaggaagctg ccccggaccg
     2221 gcaggatgaa ataccagatg agcgccgtcc ccaggaccat cgacacg
//
LOCUS       DASWNU010000519         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_460821, whole genome shotgun sequence.
ACCESSION   DASWNU010000519 DASWNU010000000
VERSION     DASWNU010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460821"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..744
                     /locus_tag="VGB57_10205"
     CDS             295..744
                     /locus_tag="VGB57_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131766.1"
                     /translation="MRNPRRKLFNKERRKIFLEWFAATANMSWSAEVAGVSYKTVSKH
                     ILKDAEFAGLVHEAMGLGVLRAKAKAIETKRTEPKIGIDGEIEVPELDMTRDEARQAV
                     REHERTLSFGRKPGRVPRIASNAEVEAAVRKRLKVFGRRVRARKGEG"
     gene            863..2212
                     /locus_tag="VGB57_10210"
     CDS             863..2212
                     /locus_tag="VGB57_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY0131767.1"
                     /translation="MSVSVGGFAGLLREAAAVFESDEDWDWFVDGLAPPVLRALHHDW
                     SWQAHEGQGAPALSDSGGEWRVWLLMAGRAFGKTYAGAQWVTEQARAHPGARIALVGA
                     SVDEVVKVMIEGPSGLIALARHDEPVEWVPTHSTLRFHSGAVAFVYSAAAGEKLRGPE
                     HHFAWADELAKWERPDACWDNLMMGLRLGTRPRCMVTTTPRPIAALRRIKALKATALT
                     EGRSFDNPHAQKAFLDWAAETYGATRLGRQELDGVLFEDVAGALFSREVLEASRQSGT
                     LPREGLKRIVVGVDPPVSAEGDACGIVVCGLGADGTAYVLADCSVSGMRPEGWAAAVA
                     RAAEAWGADRVVAEKNQGGDMVESVLRSVDAGLPVKLVSASRGKAARAEPVTAQFETG
                     RAKLAGRFPELEDELAGLTYGGGYEGPGNSPDRADAMVWAMTELARPERPMPRVTRL"
     gene            complement(2223..2453)
                     /locus_tag="VGB57_10215"
     CDS             complement(2223..2453)
                     /locus_tag="VGB57_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131768.1"
                     /translation="MPRYFFNFTAEGCEEVDLVGKTCPNDVAALQEALHAASAIMRRR
                     LDAETFCATGTIEVEDERHRTVLTLPLRAAAY"
     gene            2630..2824
                     /locus_tag="VGB57_10220"
     CDS             2630..2824
                     /locus_tag="VGB57_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131769.1"
                     /translation="MTIPEALVAVAIVSALMWAPIFGFAYARRRFGGSARNYLGAWLA
                     VSAATAASFVLLAAIETASR"
     gene            3345..>3743
                     /locus_tag="VGB57_10225"
     CDS             3345..>3743
                     /locus_tag="VGB57_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEY0131770.1"
                     /translation="MGVDTMQHDDTGLTADIELVSDKTLALTADGALVDEPDFQPIAP
                     GTPKSGSVDAGNDLDYYTLNAVAGQVYVISMNGTGPGAMPDPQIYVYNSSGAALFADD
                     EGGTASNALLTFQATYTGVYYIEAGPWFDAT"
BASE COUNT          596 a         1083 c         1488 g          576 t
ORIGIN      
        1 atgggtgagg attttgggga agaagagcgc ccctcgactg cgctcgggac gaacggggag
       61 gatgggcagg gcggtcccct tcggcgaagc tcaggacagg cttcgacagg ctcagggcga
      121 gcgggtgagg aggaggcctc ggcttcgctc ggcagtggac ctcgacttcg ctcggcccga
      181 acggaatcgg ggacgaagcc acgttcgccc ggccccgcca agcggaagaa agcgcagccc
      241 aagatcggcc ccaacgaccg cgtccatcgt gggcggaacc gggatgcgca gatcgtgcgc
      301 aatccgcggc gcaaattgtt caacaaggag cggcggaaga tctttctcga atggttcgcg
      361 gcgacggcca acatgagctg gtcggcggag gtggcggggg tcagctacaa gacggtctcc
      421 aagcatatct tgaaggatgc ggagttcgcg gggctggtgc acgaggcgat ggggctcggc
      481 gtgctgcggg ccaaggcgaa ggcgatcgag accaagcgga cggagccgaa gatcgggatc
      541 gacggcgaga tcgaagtgcc ggagctcgac atgacgcgcg acgaggcgcg gcaggcggtg
      601 cgcgagcatg agcggaccct gtccttcggc cgcaagccgg ggcgggtgcc gcggatcgcg
      661 tccaatgcgg aggtggaggc ggcggtgcgc aagaggctga aggtgttcgg gaggcgggtg
      721 cgggctcgga agggcgaggg gtgacgctgt acggggacag tatacatttt cgcttcgctg
      781 ccacggtcgg tcggccgccg ccgagtgccg cgaaaatgta tactgtcccc tttttcccct
      841 ccccgccccg agggaggaat agatgagcgt ctctgtcggc gggtttgccg ggttgctccg
      901 ggaggcggcc gcggttttcg agagcgacga ggattgggac tggttcgtgg acgggctggc
      961 gccgccggtg ctgcgggcgc tgcaccacga ctggtcgtgg caggcgcatg aggggcaggg
     1021 ggcgccggcg ctgtcggatt ccgggggcga atggcgggtc tggctgctga tggcggggcg
     1081 ggccttcggc aagacctatg cgggggcgca gtgggtgacg gagcaggcgc gggcgcaccc
     1141 gggggcgcgg atcgcgctcg tgggggcgag cgtggacgaa gtggtcaagg tgatgatcga
     1201 ggggccgagc gggctgatcg cgctcgcgcg gcacgacgag ccggtggagt gggtgccgac
     1261 gcattccacc ctccgcttcc attcgggcgc ggtggccttc gtctattcgg cggccgcggg
     1321 ggagaaattg cgcgggccgg agcatcattt cgcctgggcg gacgagcttg cgaaatggga
     1381 gcggcccgac gcctgctggg acaatctgat gatggggctt cggctgggga cgcggccgcg
     1441 ctgcatggtc acgacgacgc cgcggccgat cgcggcgttg cggcggatca aggcgctgaa
     1501 ggcgacggcg ctgaccgagg ggcgatcgtt cgacaatccg catgcgcaga aggcgttcct
     1561 ggactgggcc gcggagactt atggcgcgac gcggctcggg cggcaggagc tggacggggt
     1621 gctgttcgag gacgtggcgg gggcgctgtt ttcgcgggag gtgctggagg catcgaggca
     1681 aagtggtact ttgcctcggg aaggattgaa gaggatcgtc gtgggcgtgg atccgccggt
     1741 ttcggcggag ggggacgctt gcgggatcgt ggtgtgcggg ctgggggcgg acgggacggc
     1801 ctatgtgctg gccgactgct cggtctccgg gatgcggccg gaagggtggg cggccgcggt
     1861 ggcgcgggcg gcggaggcct ggggcgcgga ccgggtggtt gccgagaaga accagggcgg
     1921 ggacatggtc gagagcgtgc tcagaagcgt cgatgcgggg ctgccggtca agctggtctc
     1981 ggcgagccgg ggcaaggcgg cgcgcgccga gccggtgacg gcgcagttcg agaccggaag
     2041 ggcgaagctg gcgggacgct ttccggagct cgaggacgag cttgccgggc tcacttatgg
     2101 cggcggctat gaagggccgg gcaattcgcc ggacagggcg gatgcgatgg tctgggcgat
     2161 gaccgagctg gcgcggccgg agcggccgat gcctcgggtg acgcggctgt gaggtccgcg
     2221 cttcagtaag ccgcggccct cagcggcagc gtcagcaccg tgcggtgcct ttcgtcctcg
     2281 acctcgatcg tgccggtcgc gcagaaggtt tcggcgtcga ggcggcgacg catgatggcg
     2341 ctggcggcgt ggagggcctc ctggagggcg gcgacgtcgt tcgggcaggt cttgccgacc
     2401 agatcgacct cctcgcagcc ctcggcggtg aaattgaaga aataacgcgg catcgcaggg
     2461 tcaaacgcac gaaaccgcga ggcgggagca aggctcgagc ggtcggcggg gcgatcgccg
     2521 cgccgggcgg aggcgccgga ggtgagcagg tgggtctcgg gcgatctcgg cgggaggccc
     2581 gcttccggat cggcggccgc cgtggcaggc tgggaagatg ggtcacggca tgacgatccc
     2641 ggaagctttg gtcgcggtgg cgatcgtcag cgcattgatg tgggcgccga tcttcggctt
     2701 tgcttatgcc aggcggcggt tcggcggcag cgcgcgcaac tatctgggag cctggctggc
     2761 cgtctcggcg gcgacggccg cgtcattcgt gctgctcgcg gcgatcgaga ctgcgagccg
     2821 ctgacttccg gttcgtgcgg cccgatcggg cgaggggggc ggacgaaaga aaggcgcccc
     2881 cgaagctgca gggggcgcct ccttacgcgg cgcaaaggga acgccgtgga tgagacgcgc
     2941 cgggcggcga aagggttccc cggtcggcgg gcggcggggc gaggtgcgtc gcgcgccgga
     3001 tgcggccgga tcaagcggct gtaatggcag gaaggattcg ggatcggtcc ttttcggcta
     3061 cccaaaaacg tcctggcgcg actgccaaac gcttcgccgg acggctgcga gggagccgca
     3121 tttaactgtt tggattaact ttattcttgt ctggacaatg gtttaagaag tgttaataag
     3181 cacgccggac gccgaatcgc gtcccagagt ggggatacaa aaacacgcga tcgtgacgcc
     3241 cgccgcatgc ggacgccgcc tcgcctgccg cctccgccgc cgccacccag cgggacggag
     3301 ctcatgccgt gcagccattg ggcccgcagg cgcacctatt tttgatgggg gttgatacca
     3361 tgcagcatga cgacaccggc ctgaccgccg acatcgagct cgtttccgac aagacgctcg
     3421 cgctgaccgc cgacggagcg ctcgtcgacg agcccgactt ccagccgatc gcgccgggca
     3481 cgcccaagag cggctcggtc gatgccggca acgatctcga ttattacacg ctcaacgccg
     3541 tcgcggggca ggtctacgtg atctcgatga acggcaccgg ccccggcgcg atgcccgacc
     3601 cccagatcta cgtctacaac agctccggcg cggcgctttt cgccgacgac gagggcggca
     3661 ccgcctccaa cgctctgctg accttccagg cgacctatac cggcgtctat tatatcgagg
     3721 ccggcccctg gttcgacgcc acc
//
LOCUS       DASWNU010000520         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_229416, whole genome shotgun sequence.
ACCESSION   DASWNU010000520 DASWNU010000000
VERSION     DASWNU010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229416"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..840)
                     /locus_tag="VGB57_10230"
     CDS             complement(400..840)
                     /locus_tag="VGB57_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131771.1"
                     /translation="MLSACASGPAPAPAPERAPPVRQAPPVAVTPPPVETNAEDWRDQ
                     PLTPGDWSYESGEARYAGFSLRCDGARRQVVATRAGASGPLRLRTTFGERVLAPGAAL
                     PAADPLLDEMAFSRGRFTVEAEGLPTLILPAWPEPARVVEDCRG"
     gene            1063..1371
                     /locus_tag="VGB57_10235"
     CDS             1063..1371
                     /locus_tag="VGB57_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY0131772.1"
                     /translation="MRILSALLIAATALSAAASAPADAGPRERDQASAFQGARAGRII
                     PLRAIEQRIVPQMRGFAYLGPEFYADVGRYRLKFLRGQRVVWIDVDARTGEVIGKSGF
                     "
     gene            1549..2205
                     /locus_tag="VGB57_10240"
     CDS             1549..2205
                     /locus_tag="VGB57_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0131773.1"
                     /translation="MRVLIVEDEPNLGRQLRATLEGAGYAIDLATDGEDGHYLGSTES
                     YDAIILDLGLPEIDGLTVLDRWRKEGIAVPVLVLTARDSWSDKVAGLDAGADDYLAKP
                     FQTEELIARLRALIRRASGNSSSELTAGDVRLDTRSGKVTLDGEPVKLTAQEYKLLSY
                     LMHHKGKVVSRTELIEHIYDQDFDRDSNTIEVFVTRIRKKLGPDVITTIRGLGYSLED
                     "
     gene            2289..>2941
                     /locus_tag="VGB57_10245"
     CDS             2289..>2941
                     /locus_tag="VGB57_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0131774.1"
                     /translation="MPRGGSLTRRMIGIAALWIVILLGAGGYTLDRVLTTAITRNFDA
                     QLDYVLTALIASAEIAPDAEPYLNRPPADQRFLEPNSGLYFQISGEGFDPFRSRSLWD
                     RALQINGRHNDFKVHTYDSQEFGAEKLRVLERDVHLPGSPIRWRFQVAQNREELDDQI
                     TVLRKTLLRSFGILGLGLIILAALQALYGLWPLRRVRRAIAAIRSGEKSRIEDRFPR"
BASE COUNT          493 a         1060 c          914 g          474 t
ORIGIN      
        1 tcgtagagcg acgaggcgcg gccctggtgc ggagggagca ggtcggaatt cggtttttcg
       61 gtcatcgcgt tccaacgccc ggaccgggtc gccgttcctg catcgacaag ttggcccgga
      121 caagttgaag ggccgccgcg ccgagcgcga cgaccctccg aacatggtca gcggccggat
      181 ggctccagcc cggaagacct tggaaggcta ggccttcatc tcccgaacga gcaaacccga
      241 cctcttagct gaaccatgag acatcggatc acctcctttc gctttgttgt ggcgaggggg
      301 aagatgaatc atcgcgaatc gccgcgcaag acttgatttg atctttttcg gcgttagtct
      361 ttccgcttcg cccgcgcccg agggaggcgc gtgaacatct cacccccggc aatcctcgac
      421 gacccgcgcc ggctccggcc aggcgggcag gatcagggtc ggcagccctt ccgcctcgac
      481 cgtgaaccgg ccccggctga acgccatctc gtccagcaag ggatcggcgg caggaagcgc
      541 ggcgccgggc gccagcacgc gctcgccaaa agtggttcga agcctcagcg gcccggacgc
      601 tccggcgcgg gtcgcgacga cctgccggcg agcgccatcg cagcgcaggc tgaagccggc
      661 ataacgggcc tcgccggatt cgtagctcca gtcgcccggc gtgagcggct ggtcgcgcca
      721 gtcctcggcg ttcgtctcga ccggcggcgg cgtgaccgcg accggaggcg cctgccggac
      781 cggcggcgcg cgctcgggcg cgggcgccgg ggcgggcccg ctcgcgcagg cggacagcat
      841 ggccgccccc gcaattccta tcgcaatcga cctgatcatc cggcggcctc agcacggcgc
      901 cgcccgcaaa gccaagcgta aagccgaccg gcggtgcatt tcgggcggcg ggggcgccgc
      961 ccgcccgctc ccctggctga ccggcgcctg acgggcgcat tcatgggcta ttcaatgccg
     1021 ccgctctatg ccaacgcccg aacagcaaaa gcagtaaccc tcatgcgtat cctttccgcc
     1081 ctcctcatcg ccgccacggc cttgagcgca gccgcctccg cgccggccga cgccggcccg
     1141 cgcgagcgcg accaggcttc cgccttccag ggcgcccggg ccggccgaat catcccgttg
     1201 cgggccatcg agcagcggat cgtcccccaa atgcgcggct tcgcctacct cgggcccgaa
     1261 ttttacgccg acgtcggccg ctaccgcctc aagttccttc gcggccagcg agtcgtctgg
     1321 atcgacgtcg acgcccgcac cggcgaggtg atcggcaaat cgggcttttg acgggctcgc
     1381 ctgctgcgag cccgtcatcc cggcgaaggc cggggtccca gcgggaacgg ctccgcccct
     1441 ctatttccct gaggtcccgg ctttcgccgg gatgacggga gtgaaacaat tggccagcat
     1501 gccggttggg cggctattga cgcctcgata cagaaacagg ggagtgcgat gcgggtcctg
     1561 atcgtcgaag acgagcccaa tctggggcgg cagctgcggg cgacgctcga aggcgccggc
     1621 tatgcgatcg acctcgccac cgatggcgag gacggccatt atctcggctc gaccgaaagc
     1681 tacgacgcga tcatcctcga tctcggcctg ccggagatcg acggcctcac cgtgctcgac
     1741 cgctggcgca aggaaggcat cgccgttccc gtgctggtcc tgaccgcccg cgacagctgg
     1801 tcggacaagg tcgccggcct cgacgccggc gccgacgatt atctcgccaa gcccttccag
     1861 accgaggagc tgatcgcccg cctccgcgcc ctcatccgcc gcgcctccgg caacagctcg
     1921 tccgagctca ccgccggcga cgtccgcctc gacacccgct cgggcaaggt caccctcgac
     1981 ggcgagccgg tgaagctcac cgcccaggag tataaactgc tctcctacct catgcaccac
     2041 aagggcaagg tggtaagccg caccgagctg atcgagcata tttacgacca ggatttcgac
     2101 cgcgattcca acacgatcga ggtgttcgtc acccgaatcc gaaagaagct cggccccgac
     2161 gtcatcacca ccatccgcgg cctcggctat tcgctggaag attgagggtt ggaggagcga
     2221 ggtccgtcat gctgacccag tcggggtgac ggcccatgaa gcttcagcct gagctaaagc
     2281 cggaaacaat gccccgcggc ggctcgctga cccgccgcat gatcgggatc gcggcgctct
     2341 ggatcgtgat cctgctcggc gctggcggct acacgctcga ccgagttctc accactgcga
     2401 tcacccgcaa tttcgatgcg caactggact atgtgctgac tgcactcatc gcctccgccg
     2461 agatcgcgcc ggatgccgag ccctatctca accggccgcc agccgatcag cgcttcctcg
     2521 agcccaactc cggtctctat ttccagatca gcggcgaagg cttcgatcca tttcgctcac
     2581 ggtcgctctg ggatcgcgcc ttgcagatca acgggcgcca caacgacttc aaggtccaca
     2641 cctacgacag ccaggaattt ggggccgaga agctgcgcgt gctcgagcgt gacgtccacc
     2701 tgcccggctc cccgatccgc tggcgcttcc aggttgctca gaaccgcgag gagctcgacg
     2761 atcagatcac cgttctccgg aagacgcttc tccgctcctt cggcatcctc ggcctcggcc
     2821 tcatcatcct ggcagcgctc caggctctct atggcctgtg gccactccgc agggtccgcc
     2881 gggccatcgc agccattcga tcgggcgaga agtcgcgcat cgaggatcgc ttccccaggg
     2941 a
//
LOCUS       DASWNU010000521         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_72241, whole genome shotgun sequence.
ACCESSION   DASWNU010000521 DASWNU010000000
VERSION     DASWNU010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72241"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGB57_10250"
     CDS             complement(<1..237)
                     /locus_tag="VGB57_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY0131775.1"
                     /translation="MGFRCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGR
                     VAVPDPRLDEIARVAKSAQEIETQLEFVDIAGLVR"
     gene            404..1522
                     /locus_tag="VGB57_10255"
     CDS             404..1522
                     /locus_tag="VGB57_10255"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0131776.1"
                     /translation="MTFALPAQARLPAEKIDSAARAAMQRTGARGLAMAVIEDGKPVF
                     VRAWGHRNAGGDSLRADTIMYGASLTKAVFAYTVMQLVEEGRIDLDRPLAGYLPRPLP
                     EYGNLDAYGNWGDLAGDERWRKLTARHLLTHSSGFSNFSWDEPDGKLRFRFDPGARYA
                     YSGEGLILLQFAIEKGLGLDLGAEMQRRVFDRFGMKNTSMTWRPGFARNLADGWKSDG
                     KVEPHDERSRVRAAGSMDTTIADFALFAAAFMRGEGLKPSSRAEMLRAQLPITSAAQF
                     PSMAPEAPPQKRWKGLAAGLGIVAFDGPQGRGFFKGGHNDSTGNIWVCLERGRRCVVL
                     LANDVRAEAAFPELVELILGDTGLPWRWEYSWLPAGPR"
     gene            complement(1705..2310)
                     /locus_tag="VGB57_10260"
     CDS             complement(1705..2310)
                     /locus_tag="VGB57_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY0131777.1"
                     /translation="MPTRSLFASRFYEGEVGLAPLTAELETACRSLAREDLAGRRWSS
                     DHNYRGYTSYASLNDLPKRDPAFAELVRHLNRHVAAFAADCAFDLEGGRLKLDSLWVN
                     VLKPGGAHSGHIHPHSIVSGTLYVAVPAGSGALKLEDPRLPMMMAAPPRREDAPEDLR
                     TFVYAEPRPGTLFLWESWLRHEVVSGTGKGERISISFNYRL"
     gene            complement(2354..2776)
                     /locus_tag="VGB57_10265"
     CDS             complement(2354..2776)
                     /locus_tag="VGB57_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131778.1"
                     /translation="MRHLVLVALWALPAAAAVSATGVPLQALTEADVGADALRNSACY
                     AHDGPAVLLVATRKNAIVNAEGDLLLLDRLDDDGFPSGGARYGSNSFKVVISPAPGAV
                     DDTAADGRTAQSAQISVRRRGSTSSITARWSCNPSKSA"
     gene            2882..3685
                     /locus_tag="VGB57_10270"
     CDS             2882..3685
                     /locus_tag="VGB57_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0131779.1"
                     /translation="MAEQGRLAIVTGASSGIGLEMAKLAAEEGYDLIVAADTPLVDAS
                     RELAGHGIEVRNVETDLSTFAGVDRLLDAAGGRPIDLLVANAGHGLGRAFLDQDPAEW
                     RHVIDTNVVGNVYLVQQVARSMVARNQGRILITGSIAGHMAGAFQAVYNGSKAFIDSF
                     ADALVNELEETEVTVTCLKPGATDTHFFERADLKDTRVGTMPKDDPAAVARTGWEAMK
                     KGERSVIHGLHNKLQVAAAQVLGGGVSAKMHRHLAEPGTASKTDKSDPR"
     gene            complement(3824..4177)
                     /locus_tag="VGB57_10275"
     CDS             complement(3824..4177)
                     /locus_tag="VGB57_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEY0131780.1"
                     /translation="MRRLLYLCLGFASVALGAVGIVLPLLPTVPFMLLAAFFFARSSP
                     KLEAWIVEHPRFGAHVRAWRERGAISPAGKRAALFAFVFSAVLGLVLLPFPWLLAPLA
                     AALVGGSWIGSRPEA"
     gene            complement(4187..4756)
                     /gene="pth"
                     /locus_tag="VGB57_10280"
     CDS             complement(4187..4756)
                     /gene="pth"
                     /locus_tag="VGB57_10280"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0131781.1"
                     /translation="MQIWVGLGNPGASYAMHRHNVGFMAVDSIAASHDFEPPKKSFHG
                     WAQQGRIGGTRILLIKPATFMNESGRAVRAAMDFFKKEVGDLTLFHDELDLAPFKVKV
                     KTGGGTAGHNGLRSTEAHVGNAFRRVRIGIGHPGHKDRVTGHVLGNYAKAEQDRLADM
                     LGAIAAEAEWLAKGDDARFMNEVSLRLQD"
     gene            complement(4869..5558)
                     /locus_tag="VGB57_10285"
     CDS             complement(4869..5558)
                     /locus_tag="VGB57_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY0131782.1"
                     /translation="MSDQLTLSAETRERAGKGASRAMRREGRVPAVVYGNKEEPLAVH
                     VEEKALMKMLNTGHFMNSVVMIEVAGGTTARTLPKDVQFHPVTDRPLHVDFLRISEHS
                     KVHVNVPIRFTGEEQSRGIKRGGVLNAVRHELELVCDAAEIPDEIEISLAGMNIGDSL
                     HISAVTLPKGVESAITDRDFTIATIVPSSGSVMDAEDAASEAEVPADEVPATEATRGD
                     EDEDQPTQSQD"
     gene            complement(5778..>6110)
                     /locus_tag="VGB57_10290"
     CDS             complement(5778..>6110)
                     /locus_tag="VGB57_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY0131783.1"
                     /translation="TLSEGAQRALLLSVIEESAETRAQFGQMLAAWASGDLKGIARTF
                     DDETQMSPELKAALMSKRNAAWADWLARRLDRPGTAFVAVGAGHLVGEDSVQAMLKKK
                     GIRAKRVQ"
BASE COUNT          986 a         2086 c         2065 g          973 t
ORIGIN      
        1 gcgcaccagc ccggcgatgt cgacgaattc gagctgggtc tcgatctcct gagcggattt
       61 ggcgactcgg gcgatctcgt cgagccgcgg gtcgggcacc gcgacccggc cgacgttcgg
      121 ctcgatcgtg cagaagggat aattggccgc ctgcgccgcc gccgtctcgg tcagcgcatt
      181 gaacagggtc gatttgccga cattgggcag cccgacgata ccgcaacgaa aacccatgga
      241 aactccgtcg tcatgctgaa cttgtttcag catccagctc gatgaccgcc ccgatgctcg
      301 aaactggacc ctgaaacaag ttcagggtga cggctgggtc cccacctgtt acatatgtag
      361 catgatcggg aagaaatctt tggctctcgg gctggccatg gcaatgacct tcgcccttcc
      421 ggcgcaggcc cggctaccgg cggagaagat cgactccgcc gctcgcgccg ccatgcagcg
      481 caccggcgcg cgcggcctcg ccatggccgt gatcgaggac ggcaagccgg tcttcgtccg
      541 ggcctggggg catcgcaacg ccggcggcga ttcgcttcgg gcggacacga tcatgtacgg
      601 agcgtcgctg accaaggcgg tcttcgccta caccgtcatg cagctggtcg aggaggggcg
      661 gatcgacctc gaccggccgc tcgcgggata tctgccgcgt ccgcttcccg aatatggcaa
      721 tctcgacgcc tacggcaatt ggggcgacct cgccggcgac gagcgctggc gcaagctcac
      781 cgctcgccat ctcctcaccc acagctcggg cttcagcaac ttctcctggg atgagcccga
      841 cggcaagctt cgcttccgct tcgatccggg ggcccgctac gcttattcgg gcgaggggct
      901 gatcctgctc cagttcgcga tcgagaaagg cctgggcctc gatctcggcg ccgagatgca
      961 gcggcgggtg ttcgaccgct tcggaatgaa gaacacgagc atgacgtggc gacccggctt
     1021 cgcccgcaac ctcgccgacg gctggaagtc ggacggcaag gtcgagccgc acgacgagcg
     1081 cagccgggtc agggccgccg gctcgatgga caccaccatc gccgacttcg cgctcttcgc
     1141 cgccgccttc atgcgcggcg agggcctcaa gccttcatcg cgggccgaga tgctccgggc
     1201 ccaattgccg atcacgtccg ccgcccagtt tccgagcatg gcgcccgagg ccccgccgca
     1261 gaagcgctgg aagggcctgg cggcgggtct cggaatcgtc gccttcgacg ggccgcaggg
     1321 caggggcttc ttcaagggcg gccacaacga cagcaccggc aacatatggg tctgcctgga
     1381 acgaggccgc cgctgcgtcg tcctcctcgc caacgacgtt cgtgccgaag ccgcttttcc
     1441 cgagctggtc gaactgatcc tcggcgacac cggcctgccc tggcgctggg aatatagctg
     1501 gttgcctgcc ggccctcgtt gaccctcggg gccgctggca actctgctct tcctccgcgt
     1561 ctccgcgtct ccgcgtcact gcgcgcccaa aaaggaatac gcgcagaaac gcagaggcgc
     1621 agagaggcca tgccttacgg ggatgtcggc gtacaaatct cgtgcggctt cagcaggata
     1681 agcgcccttg ggccagcgcc gccgtcacag ccgatagttg aagctgatgc tgatccgctc
     1741 ccccttgccg gtcccggaaa ccacctcgtg ccggagccag ctctcccaca ggaagagcgt
     1801 gcccggcctg ggctcggcgt acacgaaggt cctcagatcc tccggcgcat cctcccggcg
     1861 cggcggcgcg gccatcatca tcggcaaccg gggatcctcg agcttcaggg cgcccgaacc
     1921 cgcgggcacc gcgacataga gggttccgga gacaatgctg tgcggatgga tatgcccgga
     1981 atgggcgccg cccggcttca ggacgttgac ccagaggctg tcgagcttca gccggcctcc
     2041 ctccaggtcg aaggcgcaat cggcggcgaa ggcggcgacg tggcggttca ggtgccgcac
     2101 cagctcggcg aaggccggat cgcgcttcgg caggtcgttc agcgaagcgt aggacgtata
     2161 gccgcgataa ttgtggtcgc tggaccagcg ccgaccggcc agatcttcgc gcgccagcga
     2221 ccggcaggcc gtttcgagct cggcggtgag cggcgcgagg ccgacctcgc cctcatagaa
     2281 tcggctggcg aaaaggctgc gcgtcggcat ggcgtccggt tagacgatcc cggccgggcg
     2341 ggcgaggcga gtttcaggcg cttttggaag ggttgcagct ccaccgtgcg gtgatgctcg
     2401 acgtgcttcc gcgcctgcgc acgctgatct gcgccgactg ggcggtccgt ccgtccgcgg
     2461 ccgtgtcgtc gacggcgccg ggcgccggcg agatgaccac cttgaagcta ttggatccgt
     2521 aacgcgcgcc gccgctcggg aagccgtcgt cgtcgagccg gtcgagcagg aggaggtccc
     2581 cctcggcatt gacgatcgca ttcttcctgg tggcgacgag gaggacggcc ggtccgtcat
     2641 gggcgtagca ggcgctgttc ctgagcgcgt cggcgccgac gtcggcttcc gtcaaagctt
     2701 gcagcggaac gccggtggcc gatacggccg cggcggcggg gagggcccag agggccacta
     2761 aaaccaggtg cctcatcgtc cgattatgcg ctcgtccggc cgctcccgca agtggcgccg
     2821 ccggaacccc tccggaccgc ccgggttgag gcggttcgac ccatctggcg aaggatcaat
     2881 catggcggaa caaggcaggc tcgcgatcgt cacgggcgcc tcgagcggca tcggactgga
     2941 aatggcgaag ctcgccgccg aagagggcta cgatttgatc gtcgccgccg acacgcctct
     3001 ggtcgatgcc tcccgcgagc tcgccggaca cggaatagag gtccgcaacg tcgagaccga
     3061 cctttccacc ttcgccggag tcgaccggct gctcgacgcc gccggcggcc gccccataga
     3121 cctgctcgtc gccaatgccg gccacggcct gggccgcgcc ttcctcgacc aggatcctgc
     3181 ggagtggcgt cacgtcatcg acacgaacgt cgtcggcaac gtctacctcg tccagcaggt
     3241 ggcccgctcg atggtcgctc gcaaccaggg acggatcctg atcaccggct cgatcgccgg
     3301 ccacatggcg ggggccttcc aggccgtcta caacggctcc aaggcgttca tcgacagctt
     3361 cgccgacgcg ctcgtcaacg agctcgagga gaccgaagtg actgtcacct gcctcaagcc
     3421 cggagccacc gacacccatt tcttcgagcg cgccgacctc aaggacacca gggtcgggac
     3481 gatgccgaag gacgatcccg ccgccgtcgc caggaccggc tgggaggcga tgaagaaagg
     3541 cgagcgaagc gtcatccacg gcctccacaa caagctccag gtcgcggcgg cgcaggtgct
     3601 cggcggcgga gtctcggcca agatgcaccg ccacctggcg gagcccggca cggcctcgaa
     3661 gacggacaag agcgatcccc gctgattttg cctccccatc ttcgatgggg agggggacca
     3721 cccgaagggt ggtggagggg tagctggcgc aagcctcgcc acttacccct ccaccatggt
     3781 tcgcatggtc cccctcccca ccgcttcgcg gcggggaggc aaatcaagcc tccggccggc
     3841 tcccgatcca gcttccgccg acgagcgcgg cggcgagcgg cgcaagcagc caggggaagg
     3901 ggaggagcac cagcccgagc acggcgctga agacgaaggc gaagagggcg gcgcgcttgc
     3961 cggcggggct gatcgcgcct cgctcgcgcc aggcgcggac gtgggccccg aaccggggat
     4021 gctcgacgat ccacgcctcg agcttcggcg agctgcgcgc gaagaagaag gcggcgagca
     4081 gcatgaacgg caccgtcgga agcagcggca ggacgatccc caccgcgccg agcgcgacgc
     4141 ttgcaaagcc caggcaaagg tagagcaggc ggcgcatcgc tgcccctcag tcctgaagcc
     4201 tgagcgagac ctcgttcatg aagcgggcgt cgtccccctt ggccagccat tcggcctccg
     4261 cggcgatcgc tccgagcatg tcggcgagcc ggtcctgctc ggctttggcg taattgccca
     4321 gcacgtggcc ggtcaccctg tccttgtggc cgggatggcc gatcccgatc cgcacccggc
     4381 ggaaggcgtt gccgacatgg gcctcggtcg agcggaggcc gttgtggccg gcggttccgc
     4441 cgccggtctt caccttgacc ttgaagggcg cgaggtcgag ctcgtcatgg aagagggtga
     4501 ggtcgcccac ctccttcttg aagaagtcca tcgccgctcg aaccgcccgg ccgctctcgt
     4561 tcatgaaggt ggcgggcttg atcagaagga tccgggtccc cccgatgcgt ccctgctggg
     4621 cccagccgtg gaagctcttc ttgggaggct cgaaatcgtg gctggcggcg atcgagtcga
     4681 ccgccatgaa gccgacgttg tgccggtgca tggcgtagct cgcgccggga ttgccgaggc
     4741 cgacccagat ctgcacgggt gagaaatccc cccaatcgtc cgtggccggg gcatccccgg
     4801 gctggcccgg cacgtcgacg gccctggggc cgaggagcgc gccgagccag cccaggatcg
     4861 acaagccgtc agtcctgcga ctgggtcggc tggtcctcgt cctcgtcgcc gcgagtcgcc
     4921 tcggtcgccg gaacctcgtc cgccggcacc tcggcctcgg acgcggcgtc ctcggcgtcc
     4981 atgacggagc cggaggaggg aacgatcgtg gcgatcgtga agtcgcgatc ggtgatcgcg
     5041 ctttcgacgc ccttggggag ggtcacggcg gagatatgca gcgaatcgcc gatgttcatt
     5101 ccggcgaggc tgatctcgat ctcgtcggga atctcggcgg cgtcgcagac cagctcgagc
     5161 tcgtggcgga cggcgttcag aacgcctccg cgcttgatgc cccgcgactg ctcttctccg
     5221 gtgaagcgga tcggcacgtt gacgtgcacc ttcgaatgct cggagatgcg aaggaaatcg
     5281 acatgcagcg gccggtcggt gaccgggtgg aactggacgt ccttgggaag ggtccgggcg
     5341 gtggtgccgc cggcgacctc gatcatcacg accgagttca tgaagtggcc ggtgttcagc
     5401 atcttcatca gcgccttttc ctcgacgtgg acggcgaggg gctcttcctt gttgccgtag
     5461 acgacggcgg ggactcggcc ttcgcgacgc attgcgcggg aggctccctt gcccgcccgc
     5521 tcgcgcgtct cggccgacag cgtaagctga tcgctcatgt gcatgtctcc aatagctgga
     5581 ttgataaaac ccgcgctcac gcctccaggg atgaccatga acggggaagc ggcgcctcta
     5641 gcggaggggg gcgcggaagg caagggaggg tggtcgggtg gatggaaggc gtcgtcccgg
     5701 cgaaagccgg gacctcgggg agcccgccgt gccctcttct cggccaggtc ccggcttccg
     5761 ccgggatgac ggggacttca ctgcacgcgt ttcgccctga tccccttctt cttcagcatc
     5821 gcctggaccg aatcctcgcc gaccagatgc cccgcgccga cggcgacgaa agccgtgccc
     5881 ggcctgtcca gccgcctggc cagccagtcc gcccaggcgg cgttgcgctt gctcatcagc
     5941 gccgccttca gctcgggcga catctgggtc tcgtcgtcga aggtccgggc gatgcccttc
     6001 aggtcgccgc tcgcccaggc cgccagcatc tgcccgaact gcgccctcgt ctcggcgctt
     6061 tcctcgatca cggaaaggag cagcgcccgc tgcgccccct cggagagggt
//
LOCUS       DASWNU010000522         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_20703, whole genome shotgun sequence.
ACCESSION   DASWNU010000522 DASWNU010000000
VERSION     DASWNU010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20703"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VGB57_10295"
     CDS             <1..1148
                     /locus_tag="VGB57_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEY0131784.1"
                     /translation="ADSARRFASAQVDNFHRRLDQVRDGDNRSSNGLALNLGFAGEDR
                     MNDPRAELAQRLGLPGGRPDDRYDLDGRPIEVVNPLAAGVSDDGGQVPGARSHAAEGE
                     GGGSAVGLWTAGGIDWGRRDGQTGERDYRFSTSGLSAGADMKVSDTLILGGGLGYGYD
                     RTKIGSDDSLSEGESWLGVIYGGWRPAEGLHISGLIGGGSLDYESRRWASAASDFAFG
                     ERSGSLMFGSLEASVDRTSGAMRWSPYARLETASVTLDAFTETGAGIYALTYDEMSFD
                     SLSSTLGVRFGWLMQGRDSTFAPAATVEWSHAFDDAGGQGVRYADWASSPLYIVPLSA
                     WSRDTLSVDLEGRWSVGSNFDLTAAYRGSVGSDMSSHGIRLRLLTRF"
     gene            complement(1321..1394)
                     /locus_tag="VGB57_10300"
     tRNA            complement(1321..1394)
                     /locus_tag="VGB57_10300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1360..1362),aa:Gln,seq:ttg)
BASE COUNT          228 a          527 c          556 g          246 t
ORIGIN      
        1 aagccgacag cgcccgccgc ttcgcctcgg cgcaggtgga caacttccat cgccgcctcg
       61 accaggtgcg cgacggcgac aaccgcagct ccaacggtct ggcgctgaac ctcggcttcg
      121 ccggcgagga tcgaatgaac gatcctcgcg ccgaactggc gcagcgcctg ggcctgcccg
      181 gcggccggcc tgacgaccgc tacgacctgg atggtcgccc gatcgaggtg gtgaacccgc
      241 tggcggccgg cgtctcggac gacggcggcc aggttcctgg tgctcgcagc catgcggccg
      301 aaggggaggg cgggggctcg gccgtgggcc tctggaccgc cggcggcatc gactggggtc
      361 gccgtgacgg ccagacaggg gagcgggact accgcttctc cacctcgggg ctcagcgccg
      421 gggcggacat gaaggtctcc gacaccctga tcctcggcgg cggcctcggc tacggctacg
      481 accgcaccaa gatcggctcg gacgactcgc tatcagaggg cgaaagctgg ctcggggtga
      541 tctacggcgg ctggcgtccg gctgaagggc tgcacatcag cggccttatc ggcggcggat
      601 cgctcgacta cgagtcgcgc cgctgggcct cggccgccag cgacttcgcc ttcggcgagc
      661 gttccggctc gctgatgttc ggctcgcttg aggcctcggt ggatcggacg agcggcgcca
      721 tgcgctggtc gccctacgcc cgcctcgaga cggcgtccgt caccctcgac gccttcaccg
      781 agaccggggc cggcatctac gccctgacct atgacgagat gtcgttcgac agcctgtcgt
      841 cgacgctggg cgtgcgcttc ggctggctga tgcagggcag ggactcgacc ttcgctcccg
      901 cggccacggt ggaatggtcc cacgccttcg acgacgccgg cgggcagggg gtgcgctatg
      961 cggactgggc gtcgagcccg ctctacatcg tgccgctcag cgcctggtcg cgcgacaccc
     1021 tgtcggtgga tctggagggc cgctggagcg tcggatcgaa cttcgacctc accgcggcct
     1081 atcgcgggag cgtcgggtcc gacatgtcca gtcacggcat ccgactgcgg ctgctgacgc
     1141 ggttctgatc ggccgcccct ccgtctgagt cggacggagg ggtgcgcccg accgggggat
     1201 cgccccgtca gttcgtcgca atgcggggat gagagggcgt cgcgcccgag ccgcttggcg
     1261 gagccccgcg aacggtgctt ccggtggagc cgaaacggcg gctaagtctt tgaaagcgct
     1321 tggctgggga actaggactc gaacctagaa tgacggtacc aaaaaccgga gtgttaccat
     1381 tacaccattc cccagcggag cggcgtcgcc gctgggccgg agccttgcgg agcggctgga
     1441 tagcgcaagc gttcgcgccg tgcaaccggt gcggaggcaa ggttcggcag acttgcttca
     1501 agtcggccag atggtcgctt gccgcctcgg atcggcgtcg ctataagccc gccctca
//
LOCUS       DASWNU010000523         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_46319, whole genome shotgun sequence.
ACCESSION   DASWNU010000523 DASWNU010000000
VERSION     DASWNU010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46319"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..551
                     /locus_tag="VGB57_10305"
     CDS             57..551
                     /locus_tag="VGB57_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0131785.1"
                     /translation="MAMNVGQENETGEESPMSDINTTPLVDVMLVLLIIFLITVPVVI
                     QTIPMKLPKVDFEPTTTKPENVSLSVRGGPGGTCEVYWGLTKMNSEDLLKRAVRKLED
                     EIKKLGGVDNMTEENMPEAHIRADVDTPYRCVGGAIFTMQRAGFARVGFISEPPPRGG
                     GARL"
     gene            589..1014
                     /locus_tag="VGB57_10310"
     CDS             589..1014
                     /locus_tag="VGB57_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0131786.1"
                     /translation="MSMAVGGNDDEPMMDINTTPLIDVMLVLLIMFIITIPLQTHAVK
                     LDLPQDQPNQQPPPIDPVKNKIVITPQNQVMWNGQQIDLVRLRQYLDVTQTMNPVPEL
                     HLQPEPNARYELVDEVLAVTKRARVEKMGFVGNEAYSTF"
     gene            complement(1232..3007)
                     /locus_tag="VGB57_10315"
     CDS             complement(1232..3007)
                     /locus_tag="VGB57_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0131787.1"
                     /translation="MADTPDETARPRRIANLALIWRFTARYKGHVAGALLALLVAAAA
                     TLAIPAAFKLIVDRGFTGTGGDIGRWFQYLLLIVVVMAFATATRFYFVSWLGERVVAD
                     EREAVQRNLRRLAPAFFEENRPSEIASRLTADTTIIEQVVGTTISVALRNIVMGLGGI
                     VYLLALAPKLTAMLLLGIPVVILPIVFLGRRIRNVSRFGQDRIAEVGAMVDEVLGAMK
                     IVQGFGQEEREASRFAAAVERVFETARRRILLRAVMTAGVIALIFGSITMVMWQGAVD
                     VQSGRISGGTIAAFVLTGALVAGAFGALTEVYGDLLRGAGAAGRLTELLREQPEIRAP
                     ARPTPLPSPGRGALEFRSVTFRYPTRREVSALHEFSLAVAPGETLAVVGPSGAGKSTL
                     FQLALRFYDPEAGTILLDGVPLPDADPAEVRARIAVVPQDIVIFAASARDNLRYGRWD
                     ASDDELWAAAETANAAEFLRKLPEGLDTYMGEGGARLSGGQRQRLAIARAVLRDAPLL
                     LLDEATSALDAESERLVQRSLERLMADRTTIVIAHRLATVRAADRIVVMEQGRIVEQG
                     NDSSLRAEGGLYARLARLQFDGIAA"
     gene            3226..>3526
                     /locus_tag="VGB57_10320"
     CDS             3226..>3526
                     /locus_tag="VGB57_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY0131788.1"
                     /translation="MLYDAYEMQRSLLAGASTLANMGAGWLQNPANPFAYSQMGPIVA
                     SALDVFAHASAPRGKPEFGLASTLVEGRRVAVHEEVVLRKPFGQLKHFRREGVEGG"
BASE COUNT          630 a         1196 c         1167 g          533 t
ORIGIN      
        1 cttcgggtcc gccgtcgccg gctccccggc actcgatcta gataggatta ccgaccatgg
       61 ccatgaatgt cggccaagag aatgaaaccg gcgaagaatc gccgatgtcc gacatcaaca
      121 cgacgccgct cgtcgacgtc atgctggtgc ttctcatcat cttcctgatc acggtgccgg
      181 tcgtcatcca gacgatcccg atgaagcttc ccaaggtcga cttcgagccg accacgacca
      241 agccggagaa cgtctctctc tccgtccgcg gcggccccgg cggcacctgc gaggtctatt
      301 ggggcctcac caagatgaac tccgaggatc tgctcaagcg cgccgtcagg aagctcgagg
      361 acgagatcaa gaagctcggc ggagtcgaca acatgaccga ggaaaatatg ccggaggcgc
      421 atatccgggc ggacgtcgac acgccctacc ggtgcgtggg cggcgccatc ttcaccatgc
      481 agcgggcggg cttcgcgcgc gtcggcttca tttccgaacc gcctccgcgg ggcggcggcg
      541 cgcgcctcta ggcggcacgg gacagcgtaa gtttaggagt tacactttat gtcaatggca
      601 gtcggcggca acgatgacga accgatgatg gacatcaaca cgacgccgct catcgacgtc
      661 atgctggtgc tcctgatcat gttcatcatc accatcccgc tccagaccca cgcggtgaag
      721 ctggaccttc cgcaggacca gcccaaccag cagccgccgc ccatcgatcc ggtcaagaac
      781 aagatcgtga tcacgccgca gaaccaggtg atgtggaacg gccagcagat cgacctggtc
      841 aggcttcgcc agtatctcga cgtcacccag acgatgaacc cggtccccga gcttcacctc
      901 cagcccgagc ccaacgcccg ctacgagctg gtcgacgaag tcctcgccgt caccaagcgc
      961 gcccgggtgg agaagatggg cttcgtcggc aacgaggcct actcgacctt ctaaaaatcc
     1021 agttttcact gtctctcagg gcggtccttc ggggccgcct ttttttgcct ccccatctcc
     1081 agatggggag ggggaccagg cgaagcctgg tggaggggta gctgccgcaa gatcaggggg
     1141 atcgcgctcg aactcaccac gtcccgagcg ctctacgcct ccaccatgct tcgcatggtc
     1201 cccctcccca tgctttgcct ggggaggcag atcaggcggc tatgccgtcg aactggagcc
     1261 gcgccaggcg ggcgtagagg ccgccttcgg cgcgcaggct ggagtcgttg ccctgctcga
     1321 cgatccggcc ctgctccatg accacgatcc ggtccgccgc ccggaccgtc gccaggcgat
     1381 gggcgatgac gatggtggtc cggtccgcca tcagccgctc gagcgagcgc tgaacgagtc
     1441 gctcgctttc agcgtccagg gccgaagtgg cctcgtcgag cagcagcagg ggcgcatcgc
     1501 gaagcaccgc ccgggcgatt gcgagacgct ggcgctggcc gcccgagagc cgggccccgc
     1561 cttcgcccat ataggtgtcg agcccttccg gcagcttcct caaaaactcg gcggcgttgg
     1621 cggtctcggc cgccgcccac aattcgtcgt ccgacgcatc ccagcgcccg tagcgcagat
     1681 tgtcgcgggc ggaggcggcg aagatcacga tatcctgggg cacgaccgcg atccgggccc
     1741 ggacctcggc ggggtccgcg tcgggcaggg gcactccatc gaggagaatt gtccccgcct
     1801 ccggatcgta gaaacgcagg gcgagctgga acagggtcga cttgccggcg ccggacggcc
     1861 ccaccaccgc cagcgtctcg cccggcgcca cggcgagcga gaattcgtga agcgcgctga
     1921 cttcgcgccg ggtcggatag cggaaggtga cagagcggaa ctcgagcgct ccgcggccgg
     1981 gggagggcag aggcgtgggc cgggccggag ccctgatctc gggctgttcg cgaagcagct
     2041 cggtgagccg gccggcggcg ccggcgccgc gcagcaggtc gccatagact tcggtcaggg
     2101 ctccgaaggc tccggcgacg agcgcgccgg tgaggacgaa ggcggcgatg gtgccgccgc
     2161 tgatccgccc cgactggacg tcgaccgcgc cctgccacat caccatcgtg atcgagccga
     2221 agatgagagc gatcacgccc gccgtcatca ccgccctcag caggatccgg cgccgcgccg
     2281 tctcgaacac ccgctccacc gcggccgcga agcggctcgc ctcgcgctcc tcctggccga
     2341 agccctggac gatcttcatg gcgccgagga cctcgtcgac catcgccccg acctcggcga
     2401 tccggtcctg gccgaaacgc gagacgttgc ggatccggcg gccgaggaag acgatcggaa
     2461 ggatcacgac cgggatcccg agcagaagca tggcggtcag cttcggcgcg agggcgagca
     2521 gatagacgat cccgccgagc cccatgacga tgttgcggag cgccacggaa atggtcgtcc
     2581 ccaccacctg ctcgatgatc gtggtgtcgg cggtcagccg cgaggcgatc tcggaagggc
     2641 ggttctcctc gaagaaggcg ggggcgagcc gacgcaggtt gcgctgaacc gcctcgcgct
     2701 cgtcggcgac cacccgctcg cccagccagg agacgaaata gaagcgggta gcggtggcga
     2761 aggccatcac gacgacgatc aggagcagat attggaacca gcggccgata tcgccgccgg
     2821 tgccggtgaa gccccggtcg acgatcagct tgaaggcagc gggaatggcg agagtcgccg
     2881 ccgccgccac cagcaaggcc agcagcgcgc ccgcgacatg gcccttgtag cgggcggtga
     2941 agcgccagat cagcgccaga ttggcgattc ggcgcggtct cgcggtctcg tccggggtgt
     3001 cggccatgag tgggcggtag cagggggaag gggggcgctc aaggaaccgt cgtcccagcg
     3061 aaggctggga ccccagcgag tggaagacgc cgagttcttc acccgccggg gccccagcct
     3121 tcgctggggc gacgagcagg ggacccatct cacctgtgga tggtttcgac ttgcaacccc
     3181 ttttccgttg acgcccacgc tgcagcgcac caagtaggtg accacatgct ctatgacgct
     3241 tatgaaatgc agcgttcgct gctcgccgga gcttcaaccc tcgccaatat gggcgccggc
     3301 tggctccaga atcccgccaa tcccttcgcc tacagccaga tgggtccgat cgtcgcgtcc
     3361 gcgctcgacg tcttcgcgca cgcctcggcg ccgcgcggaa agccggaatt cgggctcgcg
     3421 tcgacccttg tcgaaggccg gcgcgtcgcc gtccacgagg aggtggtgct gcggaagccg
     3481 ttcggccagt tgaagcattt ccgccgggaa ggcgtcgaag gcgggc
//
LOCUS       DASWNU010000524         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_72347, whole genome shotgun sequence.
ACCESSION   DASWNU010000524 DASWNU010000000
VERSION     DASWNU010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72347"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGB57_10325"
     CDS             <1..220
                     /locus_tag="VGB57_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0131789.1"
                     /translation="LLALSCDGREGVDDITEKALAAGGRETREKEDHGWMYGRAFEDP
                     DGHTWEPMWMDVAAATQAMAQPTQAADA"
     gene            313..1092
                     /locus_tag="VGB57_10330"
     CDS             313..1092
                     /locus_tag="VGB57_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0131790.1"
                     /translation="MSDARNPHGSFIWYELLTPDPDAAAAFYGEVIGWTAASAGQPGV
                     DYRLFSAGGTPVAGHMKLPDGAAEMGMRPGWLGYIGVDDVDSTVAGIVGAGGKVHMPA
                     MDMEGVGRMALVADPQGVPFYVMRGDSEEASSSFSAVETGRCSWNELSTPDPDGALAF
                     YTSQLGWEKGEVMPMGEMGGYQFINHGGGMIGAVMTAPPGRPAGWNFAFGVPDIDDAA
                     AKIAAGGGTVNYGPVEVPGGQYAVMANDPEGAAFMTVGPRN"
     gene            1119..1613
                     /locus_tag="VGB57_10335"
     CDS             1119..1613
                     /locus_tag="VGB57_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0131791.1"
                     /translation="MSNSALELSITRFIDAPPEAVYRTYTERTGQWWAPAPWKTRVLA
                     FDLRPGGRFAFAMEGPNGESHPGESVVLEAVPGERIVLTNAFAAGWEPRTTMDSDCDF
                     PSVTIVTFEREGSGTRYTARVRHWTEAAVKAHEAMGFEAGWSMVAAQLASLAEEEARE
                     PVAA"
     gene            1639..1935
                     /locus_tag="VGB57_10340"
                     /pseudo
     CDS             1639..1935
                     /locus_tag="VGB57_10340"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
     gene            1954..2397
                     /locus_tag="VGB57_10345"
     CDS             1954..2397
                     /locus_tag="VGB57_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEY0131792.1"
                     /translation="MIYAGFAPFVDEGSGSGRYVDGFVLPVPSGNRDAYREMARKAWG
                     DFQEHGALRLVEAWGDDVPEGKVTDYRRSVKQEDGEEIVYSWIEWPDKATRVAAWEKI
                     MADEKNKPDHDNMPFDGKRMFWGGFEVLVDSKAGIGLEAAEREIA"
BASE COUNT          438 a          842 c          886 g          371 t
ORIGIN      
        1 tctgctcgcc ctttcctgcg acggccgcga gggggtcgac gacatcacgg agaaggcgct
       61 cgccgccggc ggccgcgaga cccgcgagaa ggaggatcat ggctggatgt acggccgcgc
      121 cttcgaggac ccggacggcc acacctggga gccgatgtgg atggacgtcg cggcggcgac
      181 ccaggcgatg gcgcagccga cccaggccgc cgacgcctga agcgttgccg gcttgcggcg
      241 gatcgaatcg ccgccgagct taacttggat tatagggccg gccatcggac gcgcccgaag
      301 caggagcaaa tcatgtctga cgcccgcaac cctcacggca gcttcatctg gtacgagcta
      361 ctgacgccgg atcccgatgc cgcggcggcc ttctacggcg aggtgatcgg ctggaccgcc
      421 gcgagcgccg gccagcccgg ggtcgactat cgcctcttct ccgccggcgg cacccccgtc
      481 gccggccaca tgaagctgcc cgacggcgcg gcggagatgg gcatgcgccc gggctggctc
      541 ggctatatcg gcgtggacga cgtcgactcc acggtcgccg ggatcgtcgg cgcgggcggc
      601 aaggttcaca tgccggccat ggacatggag ggcgtcgggc ggatggcgct ggtcgccgac
      661 ccgcagggcg tgcccttcta cgtcatgcgc ggcgacagcg aggaagcgag cagctccttc
      721 tccgccgtcg aaacggggcg gtgcagctgg aacgagcttt ccacgcccga tccggacggc
      781 gccctcgcct tctacaccag ccagctcggc tgggagaaag gcgaggtgat gccgatgggc
      841 gagatgggcg gctaccagtt catcaaccat ggcggcggaa tgatcggcgc ggtgatgacc
      901 gccccgccgg ggcggcccgc cggctggaac ttcgccttcg gggtccccga catcgacgat
      961 gccgcggcga agatcgccgc cggcggcggc accgtgaact acggcccggt cgaggtcccc
     1021 ggagggcaat atgcggtcat ggcgaacgac cccgaaggcg ccgctttcat gaccgtcgga
     1081 ccccgcaact gagctttttc gaacgaagga gaatgaagat gagcaattcc gcactcgaac
     1141 tttcgatcac ccgcttcatc gacgcgccgc ccgaggccgt gtaccgcacc tataccgagc
     1201 gcaccgggca atggtgggcg ccggcgccct ggaagacccg agtcctcgcc ttcgacctcc
     1261 gccccggcgg acgcttcgcc ttcgcgatgg aggggccgaa cggcgagagc catccgggcg
     1321 agagcgtggt cctcgaggcg gtgccgggcg agaggatcgt cctcaccaac gccttcgccg
     1381 ccggctggga accgcgcacg acgatggaca gcgactgcga ctttccgagc gtcaccatcg
     1441 tcaccttcga gcgggaaggc tccggcaccc gctataccgc ccgggtccgc cactggaccg
     1501 aggcggccgt gaaggcgcac gaggcgatgg gcttcgaggc cggctggtcg atggtcgcgg
     1561 cgcagctcgc ctcgctggcc gaggaagagg cgcgcgagcc ggtcgctgcc tgaacatcaa
     1621 tatccaagga gagacgtcat gacctatgtc gaaggcttca ttctcgccgt cccggccggg
     1681 aaaaaggacg agtatcgccg tcacgccgcg ggcgcggtgc ccctgttcga ggagttcggg
     1741 gcgacccggc tcgtcgaggc gtgggaggac gacgtcgccg acggcaaggt caccgacttc
     1801 cgccgcgccg tccaggcgaa ggaaggcgag aaggtggtct tctcctggtt cgaatatccc
     1861 gacaaggcgg cccgcgactc cgccagcgcg aagatgatga gcgacccgcg gatggaggcg
     1921 atgggcgcct ccatgccgtt cgacggcaag cgaatgatct acgccggctt cgcgcccttc
     1981 gtcgacgagg gctccggctc cggccgctat gtcgacggct tcgtcctgcc ggtcccctcg
     2041 ggcaatcgcg acgcctatcg cgagatggcc cggaaggcct ggggcgactt tcaggagcac
     2101 ggggcgctca ggctggtcga ggcctggggc gatgacgttc ccgaagggaa agtgacggat
     2161 tatcgccgct ccgtgaagca ggaggacggc gaggagatcg tctattcctg gatcgaatgg
     2221 cccgacaagg cgacccgggt cgccgcctgg gagaagatca tggcggacga gaaaaacaag
     2281 cccgaccacg acaacatgcc gttcgacggc aagcgcatgt tctggggcgg gttcgaggtg
     2341 ctggtcgatt ccaaggccgg gatcggcctg gaagccgccg agcgcgagat cgcctgaacc
     2401 gttcaacctt cgtcatcgcg agcgaagcga ggcaatccag agcccgcggg tccgctggat
     2461 tgcttcgtcg cttcgctcct cgcaatgacg gatcgcagtg gagcatccca tgtccgacaa
     2521 gatcaccccc gtcctct
//
LOCUS       DASWNU010000525         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_383382, whole genome shotgun sequence.
ACCESSION   DASWNU010000525 DASWNU010000000
VERSION     DASWNU010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383382"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VGB57_10350"
     CDS             complement(<1..898)
                     /locus_tag="VGB57_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131793.1"
                     /translation="MIPFLLAGAGGLGLLGGGLAAYSGATARKIEKAVPRDGELIEVN
                     GQTIHFTDDGSGPAIVMIHGLGGQLRNFARPLVEDLARDYRVIRVDRPGSGYSPRAPG
                     TSARLSVQAETIAELIRILKLDRPLIVGHSLGGALALSLALNHADVVGGLALIAPLTQ
                     VQQIEEVPEVFKGLVIRSPAMRKAVAWTIATPMGMATAEKSLRAVFAPEPPPADFGTE
                     GGGLLAMRPNNFCASSADLVDLEGELEGMVERYPTLSVPVSILYARADNLLDYAKHGE
                     RTASEIPGTEIELVEGGHMLPFT"
     gene            complement(895..1707)
                     /locus_tag="VGB57_10355"
     CDS             complement(895..1707)
                     /locus_tag="VGB57_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0131794.1"
                     /translation="MKLEGRTAVVTGAASGIGRGTAQALAKRGCNLALADLDEEGLAE
                     TAGLIGQNGIKVTRHRLDVASREAVAAFPETVLAAHGRADLLFNNAGVAIGGTFEQVA
                     EEDFDWLMEINFFGVVRMTRAFLPLLRSSDSSRIINISSIFGIIAPPGQTAYSASKFA
                     VRAFSESLRRELEAEGAKIGVTVVHPGGVATSIAKNARPPRGVNDIDLEAQKERFDKF
                     LRMPPVRAGEIIVSGVEKDRPRIIVGNDARFMAVVERLAPVSYWKLLGRALK"
     gene            complement(1704..3251)
                     /locus_tag="VGB57_10360"
     CDS             complement(1704..3251)
                     /locus_tag="VGB57_10360"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0131795.1"
                     /translation="MADSGDMRAGGSEPAPSVVEHFDVLFVGAGLSGIGAAYHLQQGS
                     PGRSYIILEGREAIGGTWDLFRYPGIRSDSDMYTLGYSFRPWEEAKAIADGPSILRYV
                     NDTADRYGIRERIRFRHHVKKADWSSVEARWIVEAQRTDSGERVRFSCNWLHICSGYY
                     SYHEVYDPGFPGKESFRGTIAHPQFWPEDLDYSGRKVVVIGSGATAVTIVPEMAKSAG
                     HVVMLQRSPTYMVSRPSEDRIANGLRKVLPSRLAYGIVRWKNVLLQMIFFKLARKRPA
                     KVKEKLLEEVTKLLPKGYDVATHFTPRYNPWDQRLCLVPDSDMFEAISNGSASIVTGE
                     IDGFTEGGIRLKDGRALDADVVVTATGLKLQLAGDIAFAVDGEPRDLAKTLSYRGMMF
                     SDLPNLSYSFGYTNASWTLKADLTGGYLTRLLNHLKKTGTEIALPVREPGLEEVPFLD
                     FTSGYVQRARDVLPKQGSRKPWKLFQNYALDMISLKFASVEDGVIRFLPRGAPASRKP
                     ERQKEAA"
     gene            3397..>4126
                     /locus_tag="VGB57_10365"
     CDS             3397..>4126
                     /locus_tag="VGB57_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0131796.1"
                     /translation="MNRNVILALGASVLAMAAAAPASAQTIPEPEGAEQTGDITTGAG
                     ADQAGDAPSGTTDSAESGADADIIVTAQRRAESLQNVPIAVSAFSGEALERQQIENAS
                     DLQQSLPNVTFTKTNFTSSSFTIRGVGDLCTGFSCDSATGIHVNDMPLTSTRLFETEY
                     FDLERVEVLRGPQGTLYGRNATSGVVNFITARPNLNELQASLSAEYGNFDSIKLTGMV
                     NVPIGGIAGVRLAGYWLNRDGYIRN"
BASE COUNT          697 a         1371 c         1371 g          687 t
ORIGIN      
        1 gggtgaaggg cagcatatgg cctccttcga ccagctcgat ctcggtgccc ggaatctcgc
       61 tcgccgtccg ctcgccatgc ttggcatagt ccagcagatt gtcggcccgc gcgtagagga
      121 tgctgaccgg gacggagagc gtcgggtaac gttcgaccat gccttccagc tcgccctcga
      181 ggtccaccag atcggccgag gaggcgcaga aattgttcgg ccgcatcgcc agcaggccgc
      241 cgccttcggt gccgaaatcc gcgggcggcg gctcgggcgc gaacaccgcc ctgagcgact
      301 tctcggccgt cgccatcccc atcggcgtgg cgatcgtcca ggccaccgcc ttgcgcatgg
      361 cgggcgagcg gatgaccagc cccttgaaga cctccggcac ctcctcgatc tgctggacct
      421 gggtgagcgg agcgatgagg gcgagaccgc cgacgacgtc ggcatggttc agcgccagcg
      481 agagggccag cgctccgcct agcgaatggc cgacgatgag cggccggtcg agcttcagga
      541 tgcggatcag ctcggcgatg gtctcggcct ggacgctcag gcgcgcggag gttccggggg
      601 cgcgcggcga gtagcccgag ccgggccggt cgacccggat cacccgatag tcgcgcgcca
      661 ggtcctcgac cagcgggcgg gcgaagttgc gcagctgccc gccgagcccg tggatcatga
      721 cgatggccgg gccggagccg tcgtccgtaa aatggattgt ctggccgttc acctcgatga
      781 gctcgccgtc gcgcggcacc gctttctcga tcttgcgggc ggtcgcgccg ctgtaggcgg
      841 caaggccgcc gccgagcagg ccgaggcctc cggcgccggc gagcaggaac gggatcattt
      901 caaggccctc ccaagcagct tccaatagga taccggcgcc agccgctcga cgacggccat
      961 gaagcgcgcg tcgttgccga cgatgattcg cgggcggtcc ttctcgaccc ccgatacgat
     1021 gatctcgccg gccctgaccg gaggcattcg aaggaacttg tcgaaccttt ccttctgggc
     1081 ctcgagatcg atgtcgttga cgccgcgcgg cggccgcgca ttcttcgcga tcgaggtggc
     1141 gaccccgccg ggatggacga cggtgacccc gatcttcgcg ccttcggcct cgagctcgcg
     1201 gcgaagcgat tcggaaaagg cgcgcaccgc gaacttgctc gccgaataag cggtctggcc
     1261 gggcggggcg atgatcccga agatgctgga aatgtttatt attcgcgagg aatcgctact
     1321 tctcagcagt ggaaggaagg cccgggtcat ccgaacgacg ccgaagaaat ttatctccat
     1381 cagccagtcg aaatcctctt cggcgacctg ctcgaaggtg ccgccgatgg cgactccggc
     1441 attgttgaag aggaggtccg cacggccgtg cgcggcgagc accgtctccg ggaaggcggc
     1501 gacggcctcg cggctggcga cgtcgagccg gtggcgggtg accttgattc cgttctggcc
     1561 gatcagcccc gccgtctcgg cgagaccctc ctcgtcgaga tcggcgaggg cgaggttgca
     1621 gccgcgcttc gccagagcct gcgcggtgcc tcggccgatc ccgctcgcgg cgccggtgac
     1681 gaccgcggtt cgaccttcca gcttcatgcc gcctcctttt gccgctcggg cttgcgcgag
     1741 gccggagccc cgcggggcag gaagcggatg accccgtcct cgaccgacgc gaatttgagg
     1801 ctgatcatgt cgagcgcgta attctggaag agcttccagg gcttcctaga gccctgcttg
     1861 ggcaggacgt cccgagcccg ctgcacatag cccgaggtga aatcgaggaa cggcacttct
     1921 tcgagaccgg gctcccggac ggggagggcg atctcggtcc cggtcttctt cagatggttg
     1981 agcagccggg tgagatagcc gccggtcagg tccgccttca gcgtccacga ggcgttggta
     2041 tagccgaacg aataggacag gttcggcagg tcgctgaaca tcatcccgcg atagctgagc
     2101 gtcttggcaa ggtcgcgcgg ctcgccgtcg acggcgaagg cgatgtcgcc cgccagctgg
     2161 agcttgaggc cggtggcggt gacgacgacg tccgcgtcca gtgcccggcc gtccttgagg
     2221 cgaatgccgc cctcggtgaa gccgtcgatc tcgccggtca cgatcgaggc gctgccgttc
     2281 gagatcgcct cgaacatgtc ggagtccggc accaggcaga ggcgctggtc ccagggattg
     2341 tagcgcggcg tgaagtgggt ggcgacgtca tagcccttcg gcagaagctt cgtcacttcc
     2401 tcgagcagct tctccttgac cttcgcggga cgtttgcggg cgagcttgaa gaagatcatc
     2461 tgcagcagga cgttcttcca tcgaacgatg ccgtaggcga gcctggaggg caggaccttg
     2521 cgaagcccat tggcgatcct gtcctcggac gggcgcgaga ccatgtaggt cggcgagcgc
     2581 tgcagcatga cgacgtgtcc ggccgacttc gccatttccg gcacgatcgt caccgccgtc
     2641 gcgccgctgc cgatcacgac caccttcctg cccgaataat cgagatcctc aggccagaat
     2701 tgcgggtggg cgatggtgcc gcggaagctt tccttgccgg ggaagccggg gtcgtagacc
     2761 tcgtgatagc tgtaatagcc gctgcagatg tggagccagt tgcagctgaa gcgcacccgc
     2821 tcgccgctgt cggtcctctg cgcctccacg atccagcgcg cctcgaccga cgaccagtcg
     2881 gctttcttca catggtggcg gaagcgtatg cgctcgcgga tgccgtaacg gtcggcggtg
     2941 tcgttgacgt agcgcaggat cgacgggccg tcggcgatcg ccttggcttc ctcccagggg
     3001 cggaagctgt agcccaaagt gtacatgtcg ctgtccgagc ggatgccggg atagcggaac
     3061 aggtcccagg tccccccgat cgcctcgcgg ccttcgagga tgatgtagga gcggccgggg
     3121 ctgccctgct ggagatgata ggcggcgccg atccccgaca ggccggcgcc gacgaacagg
     3181 acgtcgaaat gctcgacgac acttggcgcg ggctcgctcc cgcccgcgcg catgtctccg
     3241 ctgtccgcca ttcccgtttt cgcctccccc gccgcaacgc ctgcgcttga caccaataag
     3301 gcgcaagggg attgtgggac aagaagcata aagttgccga tcgaaaccgg cactggggat
     3361 aatggcccgc aaagggcctc ggggaggaac atcgtaatga acaggaacgt gattctcgcg
     3421 ctcggcgcgt ccgtgctcgc catggccgca gcggcgccgg cttcggccca gaccatcccc
     3481 gagccggagg gcgccgagca gaccggcgac atcacgaccg gcgccggcgc cgatcaggcc
     3541 ggggacgctc cgagcggaac caccgatagc gccgagagcg gcgccgacgc cgacatcatc
     3601 gtcacggccc agcgtcgcgc ggaatcgctt cagaacgttc cgatcgcggt ctcggctttc
     3661 tccggcgagg cgctcgagcg ccagcagatc gagaatgcca gcgaccttca gcagagcctt
     3721 cccaacgtca ccttcaccaa gaccaacttc acctcgtcga gctttaccat ccgcggcgtc
     3781 ggcgacctct gcaccggctt ctcctgcgac agcgcgaccg gaatccacgt caacgacatg
     3841 ccgctcacct ccacccggct gttcgagacc gaatatttcg acctcgagcg ggtcgaggtt
     3901 ctgcgcggcc cgcaaggcac cctctacggc cgcaacgcca cgtcgggcgt ggtcaacttc
     3961 atcaccgccc gcccgaacct caacgagttg caggccagcc tcagcgccga atatggcaat
     4021 ttcgattcga tcaagctgac cggcatggtc aacgtgccga tcgggggaat cgccggagtc
     4081 cggctggccg gctactggct caaccgcgac ggctatatcc gcaaca
//
LOCUS       DASWNU010000526         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_229637, whole genome shotgun sequence.
ACCESSION   DASWNU010000526 DASWNU010000000
VERSION     DASWNU010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229637"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VGB57_10370"
     CDS             complement(<1..489)
                     /locus_tag="VGB57_10370"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEY0131797.1"
                     /translation="MTTLERERLGQAAAPQAREPGWYPDPLGSTAERSWDGQWMELIR
                     VPRLRLAAEALVREEAQQAPRLPLLTRMPSLQLPPLSKEKREQRSREREHLQQRLWME
                     AERQAFFQSPAGRARLAFERGQHLFQYELEIDQVQPTVIPGPVGASPQTTEDAVDILN
                     SVV"
     gene            complement(486..>1795)
                     /locus_tag="VGB57_10375"
     CDS             complement(486..>1795)
                     /locus_tag="VGB57_10375"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY0131798.1"
                     /translation="LLENETIRTALSDYLVDQLYENVDVRKELEERFPDDLKELAGPA
                     AGGLRQVAGDGAEKVLETSTAQELWEESNRTAHEQLLAVLENKKEAVSTDEGNVSLNL
                     GQLVTNFAEQVGIGEDLAQKLPSDAGQIQILQSNELKTAQDIAIAVKGLALLLSILAL
                     ACFAGAIYLSRDGRWVTVLLSGIGLIAAGFAVIVARQIAGGIVVDQLVKTESVKPAGE
                     AAWSIGTSLMTSIATTVIVVGILFLIAGWLSSPTRGAHATRRFLAPTLRLHTAYVYAG
                     LAILVGFYFLTGPSQGLRSFLTTLIVAGMAAFGIHELRRQTEEEFPESTYDDVFGRTR
                     DKVVSAVKDANIPEKVGEQASKLRLPERRRAEGEERGPEASPPSAAPAGEAPTAALPV
                     HDDDARLERLERLGGLREKGILTEEEFAAEKARLLGRDKPSDP"
BASE COUNT          233 a          599 c          647 g          316 t
ORIGIN      
        1 gaccaccgag ttgaggatgt cgacggcgtc ctcggtcgtc tgcggcgagg cgccgaccgg
       61 cccggggatc acggtcggct ggacctggtc gatctcgagc tcgtactgga acaggtgctg
      121 gccccgttcg aaggcgagcc gggcccggcc ggccgggctc tggaagaagg cctggcgttc
      181 ggcttccatc cacagccgct gctggaggtg ctcccgttcc cggctccgct gctcccgctt
      241 ctccttcgag agcggcggca gctgcagcga gggcatccgg gtgaggagag ggaggcgggg
      301 cgcctgctgg gcttcttcgc ggaccagcgc ctcggccgcc aagcgcagcc gcggcacccg
      361 gatcagctcc atccactggc cgtcccaaga gcgttcggcg gtgcttccga gcgggtcggg
      421 ataccagcct ggttcccggg cctggggggc ggcagcctgc ccgagccgct ctctctcgag
      481 cgtggtcatg ggtcggatgg tttatcacgg ccgagcaggc gagctttctc tgccgcgaac
      541 tcctcctcgg tgaggatgcc tttctcgcgc aggcccccca gtcgctccag gcgctcgagg
      601 cgggcgtcgt cgtcatgcac cggcagcgcc gcggtcggcg cctctccggc gggcgcggcc
      661 gagggcggcg acgcctccgg gcctcgttct tcgccttccg ctcgccgcct ctccggcagg
      721 cgcagcttcg aggcctgctc gccgactttc tccgggatgt tcgcgtcctt gaccgcgctg
      781 acgactttgt cgcgggtgcg gccgaagacg tcgtcgtagg tcgactccgg aaactcctct
      841 tcggtctgcc ggcgcagctc gtggatcccg aaggcggcca tcccggcgac gatcagggtg
      901 gtgaggaagg agcgcagccc ctggctcggg ccggtgagga agtagaagcc gacgaggatc
      961 gccagcccgg cgtagacgta ggcggtgtgc aggcgcaggg tcggcgccag gaagcggcgg
     1021 gtggcgtggg cgccgcgggt gggggaggag agccagcccg cgatcaggaa gaggatcccg
     1081 accacgatca ccgtcgtcgc gatgctggtc atcaacgagg tgccgatcga ccaggccgct
     1141 tcgcctgcgg gcttcacgct ctcggtcttc accagctggt cgacgacgat gccgcccgcg
     1201 atctgccggg cgacgatgac cgcgaacccg gccgcgatca ggccgatgcc gctgagcagg
     1261 acggtgaccc agcgcccgtc gcgggagagg tagatcgccc cggcgaagca ggcgagggcg
     1321 aggatcgaga gcagcagggc caggcctttg accgcgatcg cgatgtcctg cgccgttttc
     1381 agctcgttcg actggaggat ctggatctgg cccgcgtcgg acggcagttt ctgtgccagg
     1441 tcttccccga ttccgacctg ctcggcgaag ttggtgacga gctggccgag gttgaggctg
     1501 acgttgcctt cgtcggtcga gaccgcttct ttcttgtttt ccagcaccgc caggagctgt
     1561 tcgtgggcgg tccggttcga ctcttcccac agctcctgcg cggtcgaggt ttccagcacc
     1621 ttttcggcgc cgtcgccggc cacttggcgc aggccgccgg cggcgggccc ggccagctcc
     1681 ttgaggtcgt ccgggaagcg ctcttcgagt tccttgcgca cgtcgacgtt ttcgtagagc
     1741 tggtcgacca ggtagtcgct tagcgcggtg cggatcgtct cgttctccaa caggc
//
LOCUS       DASWNU010000527         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_331271, whole genome shotgun sequence.
ACCESSION   DASWNU010000527 DASWNU010000000
VERSION     DASWNU010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331271"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1702)
                     /locus_tag="VGB57_10380"
     CDS             complement(134..1702)
                     /locus_tag="VGB57_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0131799.1"
                     /translation="MEAGLALVEGQLAVAERLLRPHLKEKPTDIAAIRMMAELAGRLG
                     RYADAESLLRRALELAPGFTAARANLATILYRQNRPAEAIELLDSLLDGEPANPAHQN
                     LKAAALGRIGGYEEAIALYAEVLGRFPDQPKVWMSYGHVLKTVGRQGESVAAYRRALA
                     AAPALGEVWWSLANLKTVTFDERDIAAMTAALATPGLGDEDRLHLHFALGKALEDRGE
                     AEPAFAHYAEGNRLRRSAIRYDPDQTERQVERTIALFTPAFIAAREGQGRAAPDPIFI
                     LGMPRAGSTLIEQILASHSQVEGTMELPDIPALARRLDGRRPGANALPYPESLAGLSA
                     AELTGLGEDYLERTRIQRKTGRPRFVDKMPNNWIHAGFIHLILPKAKIVDARRHPLAC
                     TFSNFKQHFARGQGFTYDQVELGLYYRAYVRLMRHFDEVLPGRVHRVIYERMVEDPEG
                     ETRRLLDHLGLPFEPACLRPHENARAVRTASSEQVRRPINREGLDQWRPFEPWLGPLK
                     EALGPVIEAYPEAP"
     gene            complement(2096..>2863)
                     /locus_tag="VGB57_10385"
     CDS             complement(2096..>2863)
                     /locus_tag="VGB57_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0131800.1"
                     /translation="EVAAAGVLIVAVLGRITGGQVPPAPPEPDAPVAKMDWHIIRASI
                     TLVNGTMHIPRLFLAIAAISFFWSQGAILAAQFPPLVKNVLHANEQVATLFLAIFSVG
                     VAVGSVLINRLLKGRVLAIYSPASAIAMGLFICHLYYNTLSWPAQGPGLLDLDSFVRI
                     GQAEWVMFDLFGVAVSGGMFVVPLYAFLTTTVPKSETARTVAANNIVNSGGMVLAAII
                     LTGLVQLGVSIEETLLLVAVSSLGAAWLAWKLHKACD"
BASE COUNT          478 a          912 c         1032 g          441 t
ORIGIN      
        1 caggcgggcg ctgtggtttc gatgtcacgg caccatcgtc attccggcga aagccggaat
       61 ccatgaactc cgccgatcga gacggccagg atccgtgttc atggaacgcc gggtcgagcc
      121 tggcatgacg aatttatggc gcctccggat aggcctcgat caccgggcca agcgcttcct
      181 tcagcggccc cagccacggt tcgaagggcc gccactggtc gaggccctcg cggttgatcg
      241 gccggcggac ctgctccgag ctcgccgttc gcaccgcgcg cgcattctcg tggggacgaa
      301 ggcaggcggg ctcgaagggg aggccgagat gatccagcag gcgcctcgtc tcgccttccg
      361 gatcctcgac catgcgctcg tagatcaccc tgtggacccg cccgggcagc acctcgtcga
      421 aatgccgcat cagccggaca taggctcgat aataaaggcc gagctcgacc tggtcgtagg
      481 tgaagccctg gccgcgggcg aaatgctgct tgaagttgga gaaggtgcag gcgagcgggt
      541 ggcggcgcgc gtcgacgatc ttcgccttcg gcaggatcag gtggatgaag ccggcatgga
      601 tccagttgtt gggcatcttg tcgacgaacc gggggcgccc ggtcttgcgc tggatccggg
      661 tgcgctcgag atagtcctcg ccgagcccgg tcagctccgc cgccgagagc ccggcgaggc
      721 tctccggata ggggagggcg ttcgctccgg gccggcggcc gtcgaggcgg cgggcgagcg
      781 cgggaatgtc cggaagctcc atcgtgccct cgacctgaga atggctggcg aggatctgct
      841 cgataagggt ggagccggcg cgcggcattc cgaggatgaa gatcgggtcg ggcgccgcgc
      901 ggccctgccc ctcgcgcgcc gcgatgaagg cgggggtgaa gagggcgatt gtgcgctcga
      961 cctgacgctc ggtctggtcg ggatcgtagc ggatcgcgga gcgacggagc ctgttgccct
     1021 cggcataatg agcgaaggcg ggctccgcct cgcctcgatc ctcgagcgcc ttgccgaggg
     1081 cgaaatgaag gtggagacgg tcctcgtcgc cgaggccggg cgtcgccagc gccgccgtca
     1141 tcgccgcgat gtcgcgttcg tcaaaagtga ccgtcttcaa gttggcgagg ctccaccaga
     1201 cctcgccgag ggccggcgcg gcggcaagcg cacggcgata ggcggcgacg ctctcgccct
     1261 ggcggccgac cgtcttcagg acatggccgt agctcatcca gaccttgggc tggtcgggga
     1321 agcggccgag gacctcggcg taaagggcga tggcttcctc gtagccgccg atccggccga
     1381 gcgccgcggc cttcagattc tggtgggccg gattggccgg ctccccgtcg aggagggagt
     1441 cgagaagctc gatcgcctcg gcgggccggt tctggcgata gaggatggtg gcgagattgg
     1501 cgcgcgcggc ggtgaatccg ggcgccagct cgagcgcccg gcgaagcagg ctttcggcat
     1561 ccgcgtagcg gccgagccga ccggcgagct cggccatcat ccggatcgcg gcgatgtcgg
     1621 tcggcttctc cttgagatgg gggcgaagga gccgctcggc caccgccagc tgcccctcga
     1681 cgagagccag cccagcctcc atcagccgcg ggtcgcggac cgacgccgcg agggcccggt
     1741 cgacgtcgga agaggagctc gaagcctcgg ccatgacccg ggtatagggg aagggcgcga
     1801 aggattgcac ccgcccatgt gccggggcgg gacaggcccg aaacggcgga tccggcctct
     1861 tgccgggatg gtgaagagcg cgttaacccc gggccatgga ccgcctgatt cccgcctccg
     1921 aagacgtcag actcttcctc ctgaccttcg ccggcggact ggtcttcttc tccacgttca
     1981 tcgcctgaac tcctccccga gcttcctccg gggaggagga cagcccgaag ggtggtggag
     2041 gagttctttc cagcttccga agaacccctc caccatgctt ccgcgggagg attttctagt
     2101 cgcaggcctt gtgcaatttc caggcgagcc aggcggcgcc gaggcttgag acggcgacga
     2161 ggagcagggt ctcctcgatc gaaactccga gctggacgag gccggtgagg atgatcgcgg
     2221 cgagcaccat cccgcccgaa ttgacgatgt tgttcgccgc gaccgttcgc gccgtctccg
     2281 acttggggac ggtggtggtg aggaaggcgt agagcgggac gacgaacatc ccgcccgaga
     2341 cggcgacccc gaacaggtcg aacatcaccc attcggcctg gccgatgcgg acgaagctgt
     2401 cgaggtccag gagccccggt ccctgagccg gccaggagag cgtgttgtaa tagaggtggc
     2461 agatgaacag gcccatcgcg atcgccgacg ccggcgaata gattgcgagc acccggccct
     2521 tcaaaaggcg gttgatcagc accgagccga ccgcgacccc gaccgagaag atggcgagga
     2581 agagcgtcgc cacctgctcg ttggcgtgca gcacgttctt gacgagcggc gggaactggg
     2641 cggcgaggat cgcgccctgc gaccagaaga agctgatcgc agcgattgcc aggaacagcc
     2701 gcggaatgtg catcgtcccg ttcaccagcg tgatcgaggc gcggatgatg tgccagtcca
     2761 tcttcgccac cggcgcgtca ggctcgggcg gggcaggggg cacctggccg ccggtgatgc
     2821 ggccgaggac ggcgacgatc agcacgccgg ctgccgcaac ctc
//
LOCUS       DASWNU010000528         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_254434, whole genome shotgun sequence.
ACCESSION   DASWNU010000528 DASWNU010000000
VERSION     DASWNU010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254434"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGB57_10390"
     CDS             complement(<1..185)
                     /locus_tag="VGB57_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY0131801.1"
                     /translation="MTLYYLLKWLHVIGATVLLGTGAGIAFFMLMAHRTGDARVIAGV
                     ARIVVMADYIFTATAVVA"
     gene            complement(182..1474)
                     /locus_tag="VGB57_10395"
     CDS             complement(182..1474)
                     /locus_tag="VGB57_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0131802.1"
                     /translation="MRILLIGASGLIGSAIAARLRRDGHEIVAVGRGGGPAVRRVPVE
                     RWIKLDLREATTPEAWLPHLAGIDVVVNCAGVLQDSSRDSPGKIHREMPLALWRACEA
                     AGLRRVIHLSALGVDRGGITDFSRSKLEGDEALAASGLDWVILRPSVVVGRPAYGGSA
                     LFRGLASLPVFPSPAEAGPVDVVQLDDVAETVARLLGPGAPSRVALELAGPDRLAFAD
                     IVAAYRRWLGWEPARRFTAPGWLMGLAYRAGDALAWLGWRPPIRSTAGKEIRRGAIGD
                     NGPWRSATGIEPQSLADALAANPASVQERWFSRLYLLKPVAIGTFALFWLLTGLISLG
                     PGFERATYYMLAGGGGALSEPSVIAGGLADIAIACAIAWRPTAKLGLWAALGFSLFYI
                     VAGTILLPELWSDPLGPMMKIWPILALNLLCLAILEER"
BASE COUNT          298 a          607 c          605 g          213 t
ORIGIN      
        1 gccaccacgg cggtcgcggt gaagatataa tcggccatga cgacgatcct cgccacgccc
       61 gcgatcaccc tcgcgtcgcc tgtccggtgc gccatcagca tgaagaaggc gatccccgcg
      121 ccggtgccga gcagcaccgt cgccccgatc acgtgaagcc atttgagcag ataatagagg
      181 gtcatcgctc ctccagtatc gcgaggcaga gcaggttcag agccaggatc ggccagatct
      241 tcatcatcgg cccgagcggg tcggaccaaa gctccggaag caggatcgtg ccggcgacga
      301 tgtagaagag ggaaaagccc agcgccgccc aaaggccgag cttcgcggtc ggccgccagg
      361 cgatggcgca ggcgattgcg atgtcggcca gccctcccgc gatcacgctc ggctcgctga
      421 gcgcgccgcc gccgcccgcg agcatataat aagtcgcgcg ctcgaagccc gggccgagcg
      481 agatcaggcc ggtcagaagc cagaacaggg cgaaggtgcc gatcgccacc ggcttgagca
      541 gatagaggcg ggagaaccag cgttcctgca cggaggcagg gttggcggct aacgcgtcgg
      601 caagcgactg cggctcgatt cccgtcgcac tccgccaagg accattgtcg ccgatcgccc
      661 cgcgccggat ctccttgccg gcggtcgagc ggatcggcgg ccgccagccg agccaggcga
      721 gggcgtcgcc ggcgcggtag gcgaggccca tcagccagcc cggcgcggtg aagcgcctcg
      781 ccggctccca gccgagccag cgccggtagg cggcgacgat gtcggcgaag gcgagccggt
      841 ccggcccggc cagctcgagc gccacccgcg acggcgcgcc cgggcccagc agccgagcga
      901 ccgtctcggc gacgtcgtcg agctggacca catccaccgg tccagcctcc gccgggctcg
      961 ggaagaccgg aagcgaggcg aggccgcgga acagggcgct gccgccgtag gccggacgcc
     1021 cgaccaccac cgagggacgg aggatgaccc agtcgaggcc gctcgccgcc aacgcctcgt
     1081 ccccttcgag cttgctccgg gagaagtcgg tgatcccgcc tcggtcgact ccgagcgcgg
     1141 acaagtggat cacccgccga aggcccgcgg cctcgcaggc gcgccacaaa gcgagcggca
     1201 tctcgcggtg gatcttgccg ggggagtcgc ggctgctgtc ctggagcacc ccggcgcaat
     1261 tcacgacgac gtcgattccg gccagatggg ggagccaggc ttccggcgtc gtcgcctcgc
     1321 gaagatccag cttgatccag cgctcgaccg gcacccgccg gaccgccgga ccgccgccgc
     1381 ggccgacggc aacgatctcg tggccgtcgc gcctgaggcg ggcggcgatg gcggaaccga
     1441 tcaggccgct ggcgccgatg aggaggatgc gcatgctcgc ggtaacgggg aggcggcacg
     1501 ggcgttccgc tcccccgtca ttccggcgaa ggccggaatc catgaacacg ggcgggttcg
     1561 aagaatcttc ggttcatgga tcccggcctt cgccgggatg acgtgctatt cgtacctcag
     1621 ggcctggatc ggattggccc gggccaccct tatcgcgtgg ccggcgatgg tgcccaaagc
     1681 gatcgccaaa gccagccctc cggcgaggac gaacggcccc gga
//
LOCUS       DASWNU010000529         5632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_124944, whole genome shotgun sequence.
ACCESSION   DASWNU010000529 DASWNU010000000
VERSION     DASWNU010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124944"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..462)
                     /locus_tag="VGB57_10400"
     CDS             complement(34..462)
                     /locus_tag="VGB57_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0131803.1"
                     /translation="MTEEQEVAAIPLAEERVSVTKRQVETDRLRIQVSVEERQDAVPV
                     ELSHDEVEVERVPVNKAVSQVPSVRLEGSTTIIPVVEEVVIVEKRLILVEEIHVRRKS
                     VTETREIPVVLRSEQVRIDRSGDLNGGDGAVGHVRARGDA"
     gene            complement(654..1682)
                     /gene="queA"
                     /locus_tag="VGB57_10405"
     CDS             complement(654..1682)
                     /gene="queA"
                     /locus_tag="VGB57_10405"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0131804.1"
                     /translation="MRVEEFDFALPPDRIALRPASPRDSARLLVQRGDGAPEDRIVAD
                     LPHLLRPGDILIFNDTRVIPAQLEGRRGEARIGATLHKREGPREWRAFVRNSKRVREG
                     DRIDFGSGVAAIAGAKAEDGSLLLAFEGEEPVELLLERAGRMPLPPYIAGKRQADSRD
                     AEDYQTMFAARDGAVAAPTAALHFTPALMEKLAAAGVGNEVLTLHVGAGTFLPVKAED
                     TADHKMHSEWGRIDAAVADRLNAARAAGGRLIPVGTTSLRLLESAAGEDGTVRPFDGD
                     TAIFITPGYRFRATDGLITNFHLPRSTLFMLVSALMGLEAMQNAYGHAIAQGYRFYSY
                     GDSSLLLP"
     gene            complement(1691..2443)
                     /locus_tag="VGB57_10410"
     CDS             complement(1691..2443)
                     /locus_tag="VGB57_10410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0131805.1"
                     /translation="MRITTLLALLLGLFLAGSAAAQGLTVPPPTPAADPENTLLLDLS
                     SGGRVAIQLRPDVAPNHVARIKELARKGFYNGLTFHRVIDGFMAQGGDPAGDGTGGSQ
                     LPDLKAEFNGLPHVRGAIAAARQGAAPDADAATVAAAENSANSQFYIMLGSRLSLDGR
                     YTVFGRVIGGMNFVDAIEKGEPPANPSRILQASIAADNKAPPPPGQTFAAPASPVGAA
                     SPGGIAAGPAVPPPPSIRLPEAKPKAPPKKGR"
     gene            complement(2509..3009)
                     /gene="coaD"
                     /locus_tag="VGB57_10415"
     CDS             complement(2509..3009)
                     /gene="coaD"
                     /locus_tag="VGB57_10415"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0131806.1"
                     /translation="MRTGVYPGTFDPITLGHMDIIRRGTKLVDRLVIGVTTNPSKSPM
                     FSLEERMAMVRRELAGLGEEIEVVSFDSLLMDFAEREGASMIVRGLRAVADFEYEYQM
                     AGMNQQLNDRIETVFLMADVSLQPIASRLVKEIALYGGDISKFVPPAVVADVAGRVEA
                     LGRKGT"
     gene            complement(3106..4002)
                     /locus_tag="VGB57_10420"
     CDS             complement(3106..4002)
                     /locus_tag="VGB57_10420"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY0131807.1"
                     /translation="MADSALLAPAFGQVRAEVDDFLSALLEVPADPRGKLYEAMRYAA
                     IGGGKRLRPLLVVAASALFHTSRERAVRVGTAVECIHVYSLIHDDLPAMDDDDLRRGK
                     PTLHKAYDEATAILAGDSLHALAFEILADEATHEDPFVRAELIAELARAAGPSGMAGG
                     QMMDLAAAEESLDLAAVTRLQQLKTGALIGFCLDAGAIMGRVPPDRRLKLRGFARDVG
                     LAFQIADDLLDEEGQEEKTGKKVGKDRAKGKETFVTLLGRERARRQAGLLVAQAVEYL
                     KSYGSEADLLRAIARFAVERDH"
     gene            complement(4013..4261)
                     /locus_tag="VGB57_10425"
     CDS             complement(4013..4261)
                     /locus_tag="VGB57_10425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY0131808.1"
                     /translation="MSDQPEPISELSFEAALKRLEEIVRRLESGDASLDESIDLYGEG
                     DRLRQQCEARLQAAQAKIEKIQLGRDGQPGGTIPFDAD"
     gene            complement(4301..5419)
                     /locus_tag="VGB57_10430"
     CDS             complement(4301..5419)
                     /locus_tag="VGB57_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0131809.1"
                     /translation="MTSSSADPASPDASLTDPDEAYSHKGLTYPLGRRGPDKGELIAL
                     ADGVGWARLPIPGSLKHINIWVLEDGEPGSEPGTRGVALVDTGLDIPPCREAWEALFE
                     GPLAGRTVTRVICTHFHPDHIGLAGWLTERFAVRLWMTRGEWLFGRLLSSDVRDAPPS
                     EAIDYWRAAGWEEARIEAEAAKGWGRFASVVSPIPVSFVRMEEGDRLRVGGRTWRVLI
                     GSGHCPEHACLIDDEAGLMIAGDQVLPRITSNVSLSLSEPEADPLGDWLSSIEKLKGL
                     PDSLLVLPSHGEPFTGLHARLDALAFGHRDRLDALHLKLDEPRRAVDCFGTLFARQID
                     DSMLGLATGEALAHLRRLEVEGRAVSETRDGVHWYRAA"
     gene            complement(5400..>5632)
                     /locus_tag="VGB57_10435"
     CDS             complement(5400..>5632)
                     /locus_tag="VGB57_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HEY0131810.1"
                     /translation="CSQRELDGIVAKAAKAAGIEPAAQDHRLAGDREALIEELRRERD
                     TAAREAEAAHAAEEAGESAAPREPTADDLFKR"
BASE COUNT          827 a         1913 c         2000 g          892 t
ORIGIN      
        1 cgcgcgaatc gtataaggcg gtgacaatgt ggctcatgcg tccccccggg ctcgcacatg
       61 cccgacggcc ccgtcgcccc cattgagatc tccgctcctg tcaatgcgga cctgctcgga
      121 tcggagcacg accggtatct cccgggtttc ggtcaccgac ttgcggcgca catgaatctc
      181 ttcgacgagg atgaggcgtt tctccacgat cacaacttct tcgacgaccg ggataatggt
      241 ggtcgagccc tccagccgga cgctgggcac ctggctgacg gccttgttca ccggaacgcg
      301 ctcgacctcc acctcatcat gggagagctc aaccggcacg gcgtcctgcc gttcttcgac
      361 agaaacctgg atgcgcaggc ggtcggtctc gacctgacgc ttcgtgacgg agacgcgctc
      421 ctccgcgagc gggatcgcgg cgacttcctg ttcctctgtc atcttggccc tgcgcatgta
      481 ggaggtgacg gcgtaacagc ccggtaggcc attgcgttcc ggttttcggc cgccgcctcg
      541 agggcccggt tccagcaatg attgcagccc ggtcgcagct tcgcttgatc gtcacgcagc
      601 cagcccgagc cttcgcgcct tcgcgtgaac cggcctatcc accgatcgcc gagtcagggc
      661 agaaggaggc tcgaatcacc atagctgtag aagcggtagc cttgggcgat agcgtggccg
      721 taggcgttct gcatcgcctc caggcccatc agcgccgaga ccagcatgaa caaagtcgaa
      781 cgcgggaggt gaaagttggt gatcaggccg tcggtcgcgc ggaagcggta gccgggggtg
      841 atgaagatgg cggtgtcgcc gtcgaagggc cgcaccgtcc catcctcgcc ggcggcgctt
      901 tcgaggaggc ggagcgatgt ggtgccgacc gggatgaggc ggccgcccgc ggcccgggcg
      961 gcgttgaggc ggtcggcgac ggcggcgtcg atccggcccc attccgagtg catcttgtgg
     1021 tcggccgtat cctcggcctt gaccggaagg aaggtgccgg cgccgacatg gagggtcagg
     1081 acctcgttgc cgacgcccgc cgccgccagc ttttccatca gcgccggcgt gaagtggagc
     1141 gccgcggtcg gcgccgcgac cgcgccgtcc cgggccgcga acatggtctg gtaatcctcg
     1201 gcgtcgcgcg agtcggcctg gcgcttaccc gcaatgtagg gcggaagcgg cattcgaccg
     1261 gcccgttcga gcagcagctc gaccggctcc tcgccctcga aggcgaggag gaggctgccg
     1321 tcctccgcct tggcgccggc gatcgccgcg actccgcttc cgaaatcgat ccggtcgcct
     1381 tcgcggaccc ttttggagtt gcggacgaag gcgcgccatt cccgcggccc ttcgcgcttg
     1441 tggagggtgg ctccgatccg ggcctccccc cgccgcccct cgagctgggc ggggatgacc
     1501 cttgtgtcgt tgaagatcag gatgtcgccg gggcgaagaa gatgaggcag gtccgcgacg
     1561 atgcgatcct ccggcgcccc gtccccgcgc tggacgagca gccgggcgga atccctcggg
     1621 ctcgccggcc tcagcgcgat ccggtccggg ggaagcgcga agtcgaattc ttcgactcgc
     1681 atcccgcggc ttaccggccc ttcttgggag gcgccttggg cttggcttcg gggagccgga
     1741 tcgacggcgg cggcgggacg gccggcccgg ccgcgatccc gccgggcgat gccgcgccca
     1801 ccgggcttgc cggcgcggcg aaggtctggc ccgggggcgg cggcgccttg ttgtcggcgg
     1861 cgatcgacgc ctggaggatc cggctcggat tggccggcgg ctcgcccttc tcgatcgcgt
     1921 cgacgaagtt cattccgccg atcacccggc cgaacacggt gtagcgcccg tcgagggaaa
     1981 gccgggaccc gagcatgatg tagaactggc tgttggcgct attctccgcc gcggcgacgg
     2041 tggcggcgtc ggcgtccggg gcagcgccct ggcgggcggc ggcgatcgct ccgcgaacgt
     2101 gcggaaggcc gttgaactcg gccttgaggt cggggagctg ggagccgccg gtgccgtcgc
     2161 cggcggggtc gccgccctgg gccatgaagc cgtcgatgac ccggtggaag gtcagcccgt
     2221 tgtagaagcc cttgcgcgcc agctccttga tccgggcgac gtgattgggg gcgacgtcgg
     2281 gccggagctg gatcgccacc cggccgccgc tcgacaggtc gagcaagagc gtgttttccg
     2341 gatccgccgc cggggtcggc ggcggcacgg taaggccctg cgccgccgcg gaaccggcga
     2401 ggaacaggcc aagcagcagg gccagcaaag tcgtgatacg catgtttttg gatttccccg
     2461 gataaatccg ttttcgtcgc gcgatattgc gcggcgcggg cggccggatc aagtgccttt
     2521 gcggcccagc gcctcgaccc gcccggcgac gtccgccacg accgccggcg gcacgaactt
     2581 cgaaatgtcg ccgccgtaga gcgcgatctc cttgaccagg cgcgacgcga tcggctggag
     2641 cgacacgtcg gccatcagga aaacggtctc gatccggtcg ttcaattgct ggttcatccc
     2701 ggccatctga tactcatatt cgaagtcggc gacggctctc aggccccgga cgatcatcga
     2761 ggcgccttcg cgctcggcga aatccatcag cagcgaatcg aaggagacga cctcgatctc
     2821 ctcgccgagc ccggcgagct cgcggcggac catcgccatc cgctcctcaa gcgagaacat
     2881 gggcgatttg gacgggttgg tggtgacgcc gatgacgagc cggtcgacca gcttcgtccc
     2941 gcggcggatg atatccatat ggccgagcgt gatcggatcg aacgtgcccg gatagactcc
     3001 tgtcctcatc ttgctcccgt caccccggcg aaagccgggg cctcagcccg cacgtcgagc
     3061 ccttggccga ccaggttccg gctttcgccg gaatgacggg gagggtcaat ggtcccgctc
     3121 caccgcgaag cgcgcgatgg cgcgaagcag gtcggcttcc gagccgtagc tcttgagata
     3181 ttcgacggcc tgggcgacga gcagcccagc ctggcggcgg gcgcgctcgc ggccgagcag
     3241 cgtgacgaag gtctccttgc ccttggcccg gtccttgccg actttcttgc cggtcttttc
     3301 ctcctgccct tcctcgtcga gcagatcgtc ggcgatctgg aaggcgaggc cgacgtcgcg
     3361 ggcgaaaccg cgcagcttga gcctccggtc cggcggcacc cgccccatga tcgccccggc
     3421 atcgaggcag aagccgatca gggctccggt cttcaattgc tggaggcggg tgaccgcggc
     3481 gagatcgagg ctctcctcgg ccgcggcgag gtccatcatc tgcccgcccg ccattcccga
     3541 cggacccgcg gcgcgggcga gctcggcgat cagctcggcc cggacgaagg gatcctcgtg
     3601 ggtcgcctcg tcggcgagaa tctcgaaggc cagagcgtgg agggagtcgc cggcgaggat
     3661 cgcggtcgcc tcgtcatagg ccttgtgaag ggtcggcttg ccgcggcgaa gatcgtcgtc
     3721 gtccatcgcc gggagatcgt cgtggatcag cgaatagacg tggatgcatt cgaccgcggt
     3781 gccgacccgg accgcccttt cgcgggaggt gtggaagagc gcggaggcgg cgacgacgag
     3841 caacgggcgg agcctcttgc cgccgccgat cgccgcgtag cgcatcgctt cgtagagctt
     3901 gccgcgcgga tcggcgggca cctccaacaa agcggagagg aagtcgtcca cctccgcccg
     3961 gacctggccg aacgcgggcg cgagaagcgc ggaatccgcc atccgcggcg gctcagtcgg
     4021 cgtcgaaggg gatggtgccg cccggctggc cgtcgcggcc gagctggatc ttctcgatct
     4081 tcgcctgcgc cgcctgaagc cgggcctcgc attgctggcg caggcgatcg ccctcgccat
     4141 agaggtcgat cgattcgtcg agcgaggcat cgccgctctc aaggcgccgc acgatctcct
     4201 cgagccgctt cagcgccgct tcgaaggaga gctcggagat gggttcgggc tggtcggaca
     4261 tgggcggagc aatgagccgc gaggcaatcg cggtcaaggc tcaggcggcg cggtaccagt
     4321 ggaccccgtc acgggtttcg ctgaccgccc gcccttccac ctcgagccgc cgcaggtgcg
     4381 ccaaggcctc gccggtggcc aggccgagca tgctgtcgtc gatctggcgg gcgaagaggg
     4441 tgccgaagca gtcgaccgcg cgccggggct cgtcaagctt caagtggagc gcgtcgaggc
     4501 ggtcgcggtg gccgaacgcc agcgcgtcga ggcgggcgtg aaggccggtg aaaggttcgc
     4561 cgtgcgaggg caggacgaga aggctgtcgg gcaggccctt cagtttctcg atcgaggaga
     4621 gccagtcccc gagggggtcg gcctcgggct cgctgaggct tagcgagacg ttcgaggtga
     4681 tcctcggcag cacctggtcg ccggcgatca tcagccccgc ctcgtcgtcg atcaggcagg
     4741 catgttccgg gcaatggccg ctgccgatga gcacgcgcca cgtgcgcccg ccgacgcgca
     4801 ggcggtcgcc ctcctccatc ctcacgaagc tgaccgggat cgggctgacc accgaagcga
     4861 agcggcccca gcctttcgcc gcctccgcct cgatccgcgc ctcttcccag ccggcggcgc
     4921 gccagtaatc gatcgcctcg gacggcggcg cgtcccggac gtcgctcgaa agcaggcgcc
     4981 cgaacagcca ttcgccgcgg gtcatccaca gccgcaccgc gaagcgctcc gtcagccagc
     5041 cggcgaggcc gatatgatcc gggtggaaat gggtgcagat cacccgcgtg accgtgcggc
     5101 cggcgagcgg gccctcgaac agcgcctccc aggcctcgcg gcagggcggg atgtcgaggc
     5161 cggtgtcgac cagggccacg ccccgagtcc cgggctccga gccgggttcg ccgtcctcca
     5221 gcacccagat gttgatgtgc ttgagcgagc ccgggatggg aagccgcgcc cagccgaccc
     5281 cgtccgcgag agcgatgagc tcgcccttgt cgggcccgcg gcggccgagc ggataagtca
     5341 aacccttgtg gctgtaggcc tcgtcagggt cggtgaggga ggcgtccgga ctggcgggat
     5401 cagcgcttga agaggtcatc ggccgtcggc tcgcgcggcg cggcggattc gcccgcctcc
     5461 tcggcggcgt gcgcggcttc ggcctcgcgg gcggcggtgt cgcgctcgcg gcgaagctcc
     5521 tcgatcaggg cctcgcggtc gccggcgagc cggtggtcct gggccgccgg ctcgatcccg
     5581 gccgccttgg cggccttggc gacgattccg tccagctcgc gctgcgagca ca
//
LOCUS       DASWNU010000530         4767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_98477, whole genome shotgun sequence.
ACCESSION   DASWNU010000530 DASWNU010000000
VERSION     DASWNU010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98477"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2574)
                     /locus_tag="VGB57_10440"
     CDS             complement(<1..2574)
                     /locus_tag="VGB57_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY0131811.1"
                     /translation="MSSDSLVAEPAAADVQIVDALAFALVDGALPGELADFTDADRIA
                     AARFVAACALRRPAGIALVRVEPQGGALGHRRMRIGIVNDDMPFLVDSVANTLAARNL
                     TVHRLLHPVVCVTRDEDGTLTAVEPLRDAPERRESMMYLEVDRADARGRREIVADLHR
                     VLADVRAAVTDWRAMQAQMRADADAAGNEEGAALLRWFADGAMTLLGYEVERPDDQPT
                     DTLGLFRTPGEPTDPGGALDAMRYFEAGGDVPLLAKAERRASVHRRVPLDLVVVPFRE
                     GDKITGIGVHAGLWTSEGMNAPVEEVPLLRRRLQQLEQEFGFDPSGHSGKALRHAATS
                     LPHDLLVSLSMECVRQLVMTAMSLADRPRPTLVLVRSVLKGMMFAFVWLPRDELTTRR
                     RLAIGQMIEQASEGRISNWSVELGDGDLALLRYTLYVGPETPTPDIAELDRRLDEMVR
                     GWEPSVEEALGELVGPQRATRLALSYLEDFPEAYRIAVPPAEAAHDILRICALDGAGQ
                     RDARLYRQAEDGPKRLRLNTYRLAEIIPLSDAVPVFENFGFRVLEEHPTALDEGRLGY
                     VHEFLLEVRAGEAAQVLDRSELVERAIAEVLEGRAENDPFNQLLVAAALAPRDVVLFR
                     AWFRYLRQVGLSYSMVTVVEALRRAPGVAHGLIALFDALHDPKHEGPREAAVEAAERA
                     IRDGLSQVAAIDEDRILRLLHGVVSATLRTNAFTPAGREALAFKLDSARVPNLPQPLP
                     WREIWVYSPRVEGIHLRGGPIARGGLRWSDRRDDFRTEILGLMKAQLVKNAVIVPTGA
                     KGGFYPKHLPPMTDRDSWLAEGTESYRIFIRALLSVTDNIVEGQVVHPDGVTVLDGND
                     PY"
     gene            2841..3425
                     /locus_tag="VGB57_10445"
     CDS             2841..3425
                     /locus_tag="VGB57_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0131812.1"
                     /translation="MPRQADYPVDPLFLERWSPRAFDASEIPEADLLTLFEAARWAPS
                     AFNSQPWRFLYARRGEADWNRFLGLLIPFNQSWAHSASALVYILSDTLPATDKAGEPA
                     TSHTHSYDAGAAWACFALQATRLGYQAHGMSGVDFERVREELGVPERYRIEAAAAVGR
                     IGDPGTLPEKLRARETPSGRKAVEEFAFRGGWPG"
     gene            3484..4203
                     /gene="rutB"
                     /locus_tag="VGB57_10450"
     CDS             3484..4203
                     /gene="rutB"
                     /locus_tag="VGB57_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein B"
                     /protein_id="HEY0131813.1"
                     /translation="MTVDATSEGKTRRLVTLPARPEPIRVAAQETAVIVVDMQNAYAS
                     PGGYLDLAGFDIGGAAAVIGQCGKVVETARGAGLTIVYFQNGWDETYVEAGGPGSPNW
                     HKSNALKTMRARPELEGKLLARGGWDHALVEALAPHPGDIVLPKTRYSGFFNSQLDSV
                     LRSRGIRNIVLVGIATNVCVESTLRDGFFLEYFGIMLEDATHQAGPEYLQKASVYNVE
                     TFFGWVSTVADFCGAFGQIGE"
     gene            4314..4700
                     /gene="rutC"
                     /locus_tag="VGB57_10455"
     CDS             4314..4700
                     /gene="rutC"
                     /locus_tag="VGB57_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HEY0131814.1"
                     /translation="MPFESINPPQFPTPIAPYSAGTKAGGTVYVSGTLALGPGGTVLY
                     PGDAEAQTRHVLETIRITLEAAGATMADVAFNHIFLTDWAHYPAMNAVYAEYFPGAKP
                     ARYCIQCGLVKPGLVVEIASVAHIGA"
BASE COUNT          744 a         1578 c         1733 g          712 t
ORIGIN      
        1 ataagggtcg ttgccgtcga gcacggtgac tccgtccggg tgcaccacct ggccttcgac
       61 gatgttgtcg gtgaccgaga gcaaagcgcg gatgaagatc cggtagcttt cggtgccttc
      121 ggcgagccag gagtcgcggt ccgtcatcgg gggcagatgc ttgggataga agccgccctt
      181 ggcgccggtc ggcacgatca ccgcattctt gacgagctgg gccttcatca ggccgaggat
      241 ctcggtcctg aaatcgtcgc gccggtccga ccagcgcagg ccgccgcggg cgatcgggcc
      301 gccgcgaagg tggatgccct cgacccgcgg cgaatagacc cagatctcgc gccaggggag
      361 cggctggggc aggttgggca ccctggcgct gtcgagcttg aaggcgagcg cctcgcgccc
      421 ggccggtgtg aaggcgttgg tgcgcagcgt cgccgagacg actccgtgga gcagcctcag
      481 gatccggtcc tcgtcgatcg cggcgacctg ggagaggccg tcgcgaatgg cccgctcggc
      541 cgcctcgacc gcggcttcgc gcgggccttc gtgcttcgga tcgtggagcg cgtcgaacaa
      601 ggcgatgagg ccgtgggcga ctccgggtgc ccggcgcagc gcctcgacca cggtcaccat
      661 cgaataggac aggccgacct ggcgaagata gcggaaccag gcgcggaaga ggacgacgtc
      721 gcgcggggcg agagcggcgg cgacgagcaa ctggttgaac gggtcgttct cggcccggcc
      781 ctcgagcacc tcggcgatgg cgcgctcgac cagctcggag cggtcgagca cctgcgcggc
      841 ctcgccggcg cggacctcga gcaggaattc gtggacatag cccagccggc cctcgtcgag
      901 ggcggtggga tgctcctcga gcacccggaa gccgaaattc tcgaacaccg gcaccgcgtc
      961 cgagagcggg atgatctccg cgagccggta ggtgttgagc cggagccgct tcgggccgtc
     1021 ctccgcctgg cggtagagcc tggcgtcgcg ctggccggcg ccgtcgaggg cgcagatgcg
     1081 caatatgtcg tgagccgcct cggctggcgg gaccgcgatt cgataggctt cggggaaatc
     1141 ctcgagatag gacagagcga gccgggtcgc gcgctgcggc ccgaccagct ccccgagcgc
     1201 ctcctcgacc gacggctccc agccgcgcac catctcgtcg aggcggcggt cgagctcggc
     1261 gatgtcgggg gtcggcgtct cgggcccgac gtagagcgtg taacggagca gagcgagatc
     1321 gccgtcgccc agctccaccg accagttgga gatccgtccc tcggaggcct gctcgatcat
     1381 ctggccgatg gcgaggcgcc gccgggtggt cagctcgtcg cgcggcagcc agacgaaggc
     1441 gaacatcatt cccttcagaa ccgagcggac gagaaccaag gtcggccgcg gccggtcggc
     1501 gagcgacatc gccgtcatca ccagctggcg cacgcattcc atgctgaggc tgacgagcaa
     1561 atcgtgggga agcgaggtgg cggcgtggcg gagcgccttg ccgctgtggc ccgaggggtc
     1621 gaagccgaat tcctgctcga gctgctggag gcggcggcga agcagaggaa cctcctcgac
     1681 cggagcgttc atcccctcgc tggtccacag gccggcatgg actccgatgc cggtgatctt
     1741 gtcgccctcc ctgaagggca cgacgacgag gtcgagcggc acccggcggt ggaccgaggc
     1801 tcggcgctcg gccttggcga ggagcgggac gtcgccgcca gcctcgaaat aacgcatcgc
     1861 gtccagcgct ccgccggggt cggtcggctc gccgggggtc cggaacaggc cgagcgtatc
     1921 ggtgggctgg tcgtccgggc gctcgacctc gtagccgagg agggtcatcg ccccgtcggc
     1981 gaaccagcgc agcaaagcgg cgccttcctc gttgccggcg gcgtcggcgt cggcgcgcat
     2041 ctgcgcctgc atcgcccgcc agtcggtgac ggcggcacgg acatcggcga ggacccggtg
     2101 gagatcggcg acgatctcgc gccggccgcg cgcgtcggcg cggtcgacct cgagatacat
     2161 catcgattcg cggcgctcgg gcgcgtcgcg gagcggctcg acggcggtga gcgttccatc
     2221 ctcgtcgcgg gtgacgcaga cgaccggatg gaggagccgg tggacggtga ggttgcgcgc
     2281 cgcgagcgta ttggcgacgg agtcgacgag gaagggcatg tcgtcgttga cgattccgat
     2341 ccgcatccgg cgatggccga gcgccccgcc ctgcggctcg acccggacga gggcgattcc
     2401 ggccgggcgg cgaagggcgc aggccgcgac gaaccgggcg gcggcgatcc ggtcggcatc
     2461 ggtgaagtcg gcgagctcgc cgggaagggc gccgtcgacg agggcaaaag cgagggcgtc
     2521 gacgatctgc acgtcggcgg cggcgggctc cgcgacgagg ctgtccgaac tcataattgg
     2581 ctcccaaagg ggcggcgtag aggcgccgtc ttaatggtcg cgcgccctat gcccccggcg
     2641 gcacggaggg gcaacccctt ggacttgcct ccctgccgcg aagcggtggg gagggggacc
     2701 acgcgaagcg tggtggaggc ggtagatgca gccggtgccc ggggatcgcg cgggaaccgc
     2761 ccaggccctc gccctttacc cctccaccgc ctacggcggt ccccctcccc atctggcgat
     2821 ggggaggcaa aggaatgcca atgccccgcc aagccgatta ccccgtagac cccttgttcc
     2881 tcgagcgctg gtccccccgc gccttcgacg catcggaaat ccccgaggcg gacctgctca
     2941 ccctgttcga ggcggcgcgg tgggcgccgt cggcgttcaa ctcgcaaccc tggcggttcc
     3001 tctacgcccg gcggggggag gcggactgga accggttcct cggcctgctc atcccgttca
     3061 accagagctg ggcgcacagc gcctcggctc tggtgtacat cctctccgac accctgcccg
     3121 ccaccgacaa ggccggcgag ccggcgacct cgcacaccca cagctacgat gccggtgcgg
     3181 cctgggcctg cttcgcgctt caggcgaccc ggctgggcta ccaggcgcac ggaatgtcgg
     3241 gcgtggattt cgaacgggtg cgggaggagc ttggggtgcc ggagcgctat cggatcgagg
     3301 cggcggcggc ggtggggcgg atcggggatc ccgggacgct cccggagaaa ttgcgggcgc
     3361 gcgaaactcc gagcggtagg aaggcggtgg aggaattcgc gttcaggggc gggtggcccg
     3421 gataacgtcg ccgggacgac gcgtgacttg tgccgggcct ccttctggtc aacattcccg
     3481 cacatgacgg tggacgcgac cagcgaaggc aagacgagac ggctcgtcac cctccccgct
     3541 cgcccggagc cgatccgggt cgcggcgcag gagaccgcgg tcatcgtcgt cgacatgcag
     3601 aacgcttatg cctcgcccgg cggctatctc gacctcgccg gcttcgacat cggcggggcg
     3661 gcggcggtga tcgggcagtg cggcaaggtg gtcgaaaccg cccgcggcgc cggcctgacg
     3721 atcgtctatt tccagaacgg ctgggacgag acctatgtcg aggcgggcgg gccgggctcg
     3781 cccaactggc acaagtccaa tgccctgaag acgatgcggg cgcggccgga gctcgagggt
     3841 aaattgctcg cgcggggcgg ctgggaccat gcgctggtcg aggcgctggc gccgcatccc
     3901 ggcgacatcg tccttcccaa gacccgctat tccggcttct tcaactcgca gctcgattcg
     3961 gtgctccgct cgcgcggcat acgcaacatc gtgctggtcg ggatcgccac caacgtctgc
     4021 gtcgaatcga ctttgcggga cggcttcttc ctcgaatatt tcgggatcat gctggaggac
     4081 gccacccatc aggccgggcc ggaatatctt cagaaagcga gcgtctacaa tgtcgagacc
     4141 ttcttcggct gggtctcgac cgtcgccgat ttctgcggcg cgttcgggca gatcggggag
     4201 tgatccgtca ttgcgaggag cgtagcgacg acgcaatcca gtcgggcgcc ggagcagctg
     4261 gattgcttcc cccggatcag gcccggggtc gcaatgacgg aggagaggaa taaatgccgt
     4321 tcgaatcgat caacccgccg caattcccga cccccatcgc gccttattcg gcggggacga
     4381 aggcgggcgg cacggtctac gtgtcgggca cccttgcgct cggcccgggc ggaaccgtgc
     4441 tctatccggg cgacgccgag gcgcagacgc ggcacgtgct cgagacgatc cggatcacct
     4501 tggaggccgc gggcgcgacc atggccgacg tcgcattcaa ccacatcttc ctgaccgact
     4561 gggcccatta tccggcgatg aacgccgtct atgccgaata ttttccgggc gccaagccgg
     4621 cgcgctactg catccagtgc gggctggtga aacccggcct cgtcgtcgag atcgccagcg
     4681 tggcgcatat cggcgcgtga tcggcggcat cgacttcgag gagcgcggcc cggccgatgc
     4741 accggccctg atcctgtccc cggggct
//
LOCUS       DASWNU010000531         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_46581, whole genome shotgun sequence.
ACCESSION   DASWNU010000531 DASWNU010000000
VERSION     DASWNU010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46581"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..983)
                     /locus_tag="VGB57_10460"
     CDS             complement(87..983)
                     /locus_tag="VGB57_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0131815.1"
                     /translation="MTDLILQTLLPATHDLGGFKVHRTLPHRERTTIGPFIFFDQMGP
                     AHLPEGGGIDVRPHPHINLATVTYLFAGAIDHRDSLGTFATIEPGAVNLMTAGRGIAH
                     SERSPAHLRPGGPELSGIQTWIALPRAKEEIDPAFEHVPKDRLPVVDGGGASARVVMG
                     TLWGATSPVTCHSETLYADIQLQPGGAMPVDAGADERAVYVAEGEASLDGLKLERSVL
                     YVLAPGVAATLRSATGAHVMLCGGAPIDGPRHVWWNFVSSSRDRINQAKEDWKAGNFP
                     KVPNDDKEWIPIPEVPKTVSYP"
     gene            complement(1113..1397)
                     /locus_tag="VGB57_10465"
     CDS             complement(1113..1397)
                     /locus_tag="VGB57_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY0131816.1"
                     /translation="MTEAFTGRIAPEIERRLRTALQPAQLVVTNDSARHRGHAGDDGS
                     GESHFTVEVVAERFAGMSRVERQRAVNTALADLLKDRIHALAIKARAPGE"
     gene            1437..2027
                     /locus_tag="VGB57_10470"
     CDS             1437..2027
                     /locus_tag="VGB57_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0131817.1"
                     /translation="MASASGRQTRFHGRAEARGICAHPGCEAAGEFRAPAGRGPSFDG
                     PGDWRWLCLDHVRKFNAGYNYFEGMSSDEIQAAHNPYGGWDRETRAFAHMGSDGPPKW
                     SDFQDPLDAIGARFRRAAAEGPRRDGRELSEPDRKSLRVLGLGTDADRRTLRRRYADL
                     LRRYHPDHNGGDRGHEKALQSVIEAYTHLKSRPAFA"
     gene            2049..>2148
                     /locus_tag="VGB57_10475"
     CDS             2049..>2148
                     /locus_tag="VGB57_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEY0131818.1"
                     /translation="MSDLPNANPASRGAVIMDAPDQMRKVRDLFGID"
BASE COUNT          328 a          731 c          746 g          343 t
ORIGIN      
        1 ggggtggggc agtgcggcag gcaaaggctc ggtgagacgg acaggcccca cccccagccc
       61 ctcccctgaa ggggaggggc ttggaattac ggatagctca ccgtcttcgg cacctcgggg
      121 atcgggatcc attccttgtc gtcgttgggc accttgggga aattccccgc cttccagtct
      181 tctttggcct gattgatccg gtcgcggctc gacgagacga agttccacca gacgtggcgt
      241 ggaccgtcga tcggcgcccc gccgcacagc atgacgtggg cgcccgtcgc cgagcgcagg
      301 gtcgccgcga ccccgggggc gaggacgtag aggacgcttc gctcgagctt cagcccgtcg
      361 aggctcgctt ccccttccgc gacgtagacg gcgcgctcgt cggccccggc gtcgaccggc
      421 atcgcgcctc cgggctggag ctggatgtcg gcatagaggg tctcgctgtg gcaggtgacc
      481 ggcgaggtcg caccccacag cgtccccatc acgaccctgg cgctggcgcc gccgccgtcg
      541 acgaccggaa gccggtcctt cggcacatgc tcgaaggcgg ggtcgatctc ctccttcgcc
      601 ctgggaagcg cgatccaggt ctggatcccg ctcagctccg gcccgcccgg gcgcagatgc
      661 gccggcgagc gctccgaatg ggcgatcccg cggcccgccg tcatcaaatt gaccgcgccc
      721 ggctcgatcg tcgcgaaggt gccgagcgaa tcgcgatggt cgatggcgcc ggcgaacaga
      781 taggtgacgg tggcgaggtt gatgtgcgga tgcggccgga cgtcgatccc gccgccctcg
      841 ggaagatgcg cggggcccat ctggtcgaag aagataaagg gcccgatcgt ggtccgctcg
      901 cggtgcggaa gggtgcggtg gaccttgaac ccgccgagat cgtgggtggc gggaaggagc
      961 gtctggagga tgaggtcggt catggcgcga catgtaccgg agagggaggg taatcgtcac
     1021 ccccaagccg tcatcccggc gcaagccggg gacctcaggt caagaagtct cggcgggcct
     1081 ccccgaggtc ccggctctcg ccgggatgac gatcattccc ccggcgccct cgccttgatc
     1141 gccagggcgt ggatccggtc cttcagcaaa tccgcaagcg ccgtgttcac cgcccgctgc
     1201 ctttccaccc ggctcatccc ggcgaatcgc tccgccacca cctccacggt gaaatggctc
     1261 tcgccgctgc cgtcgtcgcc ggcatggccg cggtggcggg cgctgtcgtt ggtcacgacg
     1321 agctgtgcgg gttggagggc ggtgcgcagc cggcgctcga tctcgggggc gattcggccg
     1381 gtgaaagctt cggtcatcgc ccctatatag gccgtttggc ttggaaggtg agtgcgttgg
     1441 cgagcgcaag cggaagacag acgaggtttc acggacgtgc cgaggcgcgc ggcatctgcg
     1501 cgcacccggg ttgcgaggcc gcgggcgagt tccgcgcgcc cgccggccgc ggtccgagct
     1561 tcgacggccc cggcgactgg cgctggctgt gcctcgacca tgtccgcaag ttcaacgcgg
     1621 gctacaatta tttcgagggc atgagcagcg acgagatcca ggccgcgcac aatccctatg
     1681 gcggctggga ccgcgagacc cgcgccttcg cccatatggg ctccgacggg ccgcccaaat
     1741 ggtccgattt ccaggatccg ctcgacgcga tcggcgcccg cttccgccga gccgcggcgg
     1801 aagggccgag gcgcgacggc cgcgagttga gcgagccgga tcgcaagtcg ctacgggtcc
     1861 tcggtctcgg caccgacgcc gaccgtcgga ccctgcgccg ccgctatgcc gatctgcttc
     1921 gccgctacca tcccgatcac aatggcggcg accgcggcca cgagaaggct ttgcagtcgg
     1981 tgatagaggc ttatacgcac ctcaagtccc gcccggcctt cgcctgattc cctgcctaga
     2041 gagcaaccat gtccgatttg cccaatgcca accccgccag ccgcggcgcc gtgatcatgg
     2101 atgcgcccga tcagatgcgg aaggtgcgcg acctgttcgg gatcgaca
//
LOCUS       DASWNU010000532         5805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_489461, whole genome shotgun sequence.
ACCESSION   DASWNU010000532 DASWNU010000000
VERSION     DASWNU010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489461"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VGB57_10480"
     CDS             <1..1023
                     /locus_tag="VGB57_10480"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210354.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0131819.1"
                     /translation="MGDKVEAKRTAAKLGLPLVPGSDGPVEGPEEARRVAAQTGYPLL
                     VKAASGGGGRGMKVVRSEDELEAQISQARSEAKNAFGDDTVYIEKYLGDPRHIEFQVF
                     GDGNGHAIHLGERDCSLQRRHQKVLEEAGSPVITPQERDRMGGIVAKAMADMSYRGAG
                     TIEFLYENGEFFFIEMNTRLQVEHPVTEYITGIDLVREQIRIAAGEPLSCTQDQVQFR
                     GHAIECRINAEDPETFVPSPGTVKGFHAPGGMHVRVDSGLYVGYKVPPYYDSMIAKLI
                     VYGSTRERCIMRLRRALEEFVIEGMKTTIPLHQKLINEPDFLAGDYTIKWLEEWLGKQ
                     TTRGRR"
     gene            1241..1582
                     /locus_tag="VGB57_10485"
     CDS             1241..1582
                     /locus_tag="VGB57_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131820.1"
                     /translation="MTDNKFQFSVAPEKLWQAINPWTFMNQGAQFGLVNVNLGQTPRP
                     DIEQKVLDEVGSYGRQLGRIGDALEVLIDHVRLDGLSREEADALAILKGQLAEIRKVK
                     QRERAGEGLEE"
     gene            1579..1914
                     /locus_tag="VGB57_10490"
     CDS             1579..1914
                     /locus_tag="VGB57_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEY0131821.1"
                     /translation="MKATIWHNPDCGTSRKTLEILRESGADVTVVEYLRTPPERDELR
                     RLYERAGISPAQGLRRKAEGAADVAEAGDEAVLDAMIGNPVLIERPLVETEKGVRLCR
                     PQDKVREIL"
     gene            1968..2702
                     /gene="aat"
                     /locus_tag="VGB57_10495"
     CDS             1968..2702
                     /gene="aat"
                     /locus_tag="VGB57_10495"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY0131822.1"
                     /translation="MAAIDPDLLLRAYAIGVFPMSDARDAPEVFWVEPRRRAILPLDR
                     FRLSRSLRKTIRSGEYRVTRDTAFAEVVRRCAEREETWINADIEQSYGLLHARGHAHS
                     IECWHEGELAGGLYGVSLGAAFFGESMFSTRRDSSKVALAWLVARLKVGGYRLLDCQF
                     MTDHLRSLGAVEVTQEDYLALLDTALSAGAPDGSGAGAVAAGAEARRVGAEFGALDRL
                     LAADAARTVDSPASGCVIAQLLGQTS"
     gene            complement(2738..3133)
                     /locus_tag="VGB57_10500"
                     /pseudo
     CDS             complement(2738..3133)
                     /locus_tag="VGB57_10500"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
     gene            complement(3130..3516)
                     /locus_tag="VGB57_10505"
     CDS             complement(3130..3516)
                     /locus_tag="VGB57_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY0131823.1"
                     /translation="MGVLKKLFTWWDGATLTTSLHLRRHGRKVGSDSSGNVYYASKKG
                     DRRFVSYEGANDPSRIPPEWYSWLHHQIDDVPDQSLPPPPKFLREPEPNRTGTPLAYR
                     PSGALERGAQRPAASGDYEAWTPDQA"
     gene            3788..4333
                     /locus_tag="VGB57_10510"
     CDS             3788..4333
                     /locus_tag="VGB57_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131824.1"
                     /translation="MEFPAKSLRACCALALLPFIGAAPLTVAQAQASTPPLIWQQAGA
                     IRILCLVTAPSTRRDETDKLQEELCTIVRSIVSDGSPVPVSQVGFGDPAVLEPGTVTL
                     LVHASLQRDGDQRLVALDVRPFRPGGDHNVVLFGPAPRAVRIPGTGAGGNKLEAAIAA
                     ALAEILPWRQDRNHHNINRLN"
     gene            4345..>5805
                     /locus_tag="VGB57_10515"
     CDS             4345..>5805
                     /locus_tag="VGB57_10515"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0131825.1"
                     /translation="MTQQVTNQAEEDTDYPVVDINDIIALYANVDGTGGTISNKPVYT
                     LDQVIANMNRTSYPGSGIPGPQWNYGEDFMGQNKSGDPSIITFGFQTTTSVAQAPYAF
                     YDNAGNLVAARSQYFGFQPFSAAQMDATRKAIGLWDDLVAVSFVEVANVANADLTYGN
                     VTNQPNTQAYAYLPYNYSGQSSGLEGDVWVGASALSNFQLGNGFYGLATLIHETGHAI
                     GLQHPGAYNAAPGLSITFPVNAEYYQDTRMYSQMSYFNAEFSGGGHIDWNRLNWVYGQ
                     TPLLHDIATIQAIYGADPTTRVGDTVYGFNSTAGKEVYDFSTNTMPVISIYDAGGIDT
                     LDFSGWNTKSIIDLNAGAFSDGGGSGVVPLDVLKARGLLAPSYTEAQYTALRARYNAV
                     DGMLHQNISIAYGTTIENATGGGGDDLIIGNGVDNVLIGNAGNDNIEGRLGNDTINGG
                     VGLDTMLGGAGNDLYFVDNLGDTVVEEAGGGSDRVES"
BASE COUNT         1065 a         1957 c         1860 g          923 t
ORIGIN      
        1 atgggcgaca aggtcgaggc caagcggacc gcggccaagc tcggcctccc gctggtcccc
       61 gggtccgacg gcccggtcga gggccccgag gaagcgcgcc gggtggcggc ccagaccggc
      121 tatcccctgc tcgtcaaggc agcgtcgggc ggcggcgggc gcggcatgaa ggtcgttcgc
      181 tcagaggacg agctcgaggc gcagatcagc caggcgcggt cggaagccaa gaacgcgttc
      241 ggcgacgaca ccgtctatat cgagaaatac ctcggcgacc cgcgccacat cgagttccag
      301 gtgttcggcg acggcaacgg ccatgccatc catctcggcg agcgcgactg ctcgctgcaa
      361 cgccgccacc agaaggtgct cgaggaagcg ggctcgcccg tcatcactcc gcaagagcgc
      421 gaccgcatgg gcgggatcgt cgccaaagcg atggccgaca tgagctatcg cggcgccggc
      481 acgatcgagt tcctctacga gaatggcgag ttcttcttca tcgagatgaa cacccgcctc
      541 caggtcgagc atccggtcac cgaatatatc accggcatcg acctcgtccg cgagcagatc
      601 aggatcgccg ccggcgagcc cttgtcctgc acccaggacc aggtccagtt ccgcggccac
      661 gccatagaat gccggatcaa cgccgaggac ccggagacct tcgtgccgtc gcccggcacg
      721 gtcaaaggct tccacgcgcc gggcgggatg cacgtccggg tcgatagcgg cctctacgtg
      781 ggctacaagg tgccgcctta ttacgacagc atgatcgcca agctgatcgt ctacggctcc
      841 acccgcgaac gctgcatcat gcggctgagg cgggcgctcg aggagttcgt gatcgagggc
      901 atgaagacga ccatcccgct ccaccagaag ctgatcaacg aaccggactt cctggcggga
      961 gattacacga tcaaatggct ggaggagtgg ctcggaaagc agacaacccg tggccgaaga
     1021 tgaacctgct gaccaagatc cggtagaacc cgcagcaggg cctcgaagag gcaatctggg
     1081 cagacgacac taaggcagct gtgctcctcc atgcgccgac ttggcagagc aaacctgctc
     1141 gcaagctttg gcgagcaacg attggaaaaa aggattgatt agcggcaatg agagccgttg
     1201 agcagtctcc agctgcgtag aaacccatcc ggagaccatg atgaccgaca acaagttcca
     1261 attctccgtc gcacccgaga aactgtggca ggcgatcaat ccctggacct tcatgaacca
     1321 gggcgcgcag ttcgggctgg tcaacgtcaa tctggggcag acgccgaggc ccgacatcga
     1381 gcagaaggtg ctcgacgagg tcggcagcta cgggcgccag ctgggaagga tcggggacgc
     1441 gctcgaggtg ctcatcgacc atgtcaggct cgacgggctc agccgggagg aggcggacgc
     1501 cctcgccatc ctcaagggcc agctcgccga gatccgcaag gtcaagcaac gcgaacgtgc
     1561 gggcgagggc ctcgaagaat gaaggcgacg atctggcaca accccgattg cggcacctcc
     1621 cgcaagacgc tggagatctt gcgggagagc ggcgccgacg tgaccgtcgt cgaatatctc
     1681 aggaccccgc ccgagcgcga cgagcttcgg cgcctctacg agcgcgccgg catctcgccg
     1741 gcccaggggc tgaggcgcaa ggcggaaggc gcggccgacg tggcggaggc gggcgacgag
     1801 gccgtgctcg acgcaatgat cggcaacccc gtcctgatcg agcgccccct ggtcgagacc
     1861 gagaagggcg tgcggctgtg ccggccccag gacaaggtcc gcgaaatcct ctgatccgga
     1921 gaaatccacg gcttctgtcc gccgccgcgt cgagctagag cgcggcgatg gcggcgatcg
     1981 atccggacct cttgctgcgc gcctatgcga tcggggtgtt cccgatgtcg gacgcccgcg
     2041 acgctccgga agtcttctgg gtcgagcccc ggcggcgggc gatcctcccg ctcgatcgct
     2101 ttcgactgtc gcgctcgctt cgcaagacga ttcgctcggg cgagtatcgg gtcactcgcg
     2161 acaccgcctt cgccgaggtg gtccggcgct gcgcggagcg cgaggagacc tggatcaacg
     2221 ccgatatcga gcagagctac ggccttctcc acgcccgcgg ccacgcccat tcgatcgaat
     2281 gctggcacga gggggagctc gccggcggcc tctacggagt aagcctcggc gccgcctttt
     2341 tcggcgagag catgttctcg accaggcgcg actcgtccaa ggtcgcgctc gcctggctgg
     2401 tggcgaggct gaaagtgggt ggctaccggc tgctcgactg ccagttcatg accgaccatc
     2461 tgcgcagcct cggagcggtc gaggtcactc aggaggatta tctggcgctg ttggataccg
     2521 cgctgtcggc gggagccccg gacggctccg gagccggggc cgtcgcggcc ggggccgagg
     2581 cgcgccgggt cggcgccgaa ttcggcgctc tcgaccggct gctcgccgcc gacgccgccc
     2641 ggaccgtcga ctcgcccgcc tccggatgcg tcatcgcgca gctcttgggc cagacgtcgt
     2701 aaatcgggtg ctcgacgacg ttgagcgaag gagtctcctt gtacagccag ccggagaaga
     2761 cccgctgcca gcgcccctgc gggttgcgga cgtcgagctg gacgaaggcc ccggtcagcg
     2821 cctgcacctc ccagggcgcg gtcgtctcgc aggctcgaag cctcacgatc gcgtccccca
     2881 cccgaaccgc ctggccgggc ctgagggtga tggggcgcga gattccgttg cgcttgttga
     2941 gaagcccgat caccgccacc cgctccgcca tcggagtcgt ccccggagcc acggcggtca
     3001 cggtttgcgg aatgtcgacg gtccgcggct ctccgaccgc ctgccgattg ccgccggctt
     3061 tctgttcgcc cccccggtcg cagccggcga gcgcggccgc aaggacgagg atcgcgccga
     3121 gcggaagcct catgcctggt ccggggtcca cgcctcatag tcgccgctcg ccgccgggcg
     3181 ttgcgcgccc cgctccagcg cgcccgaggg acggtaagcg agcggggtgc cggtgcgatt
     3241 gggctccggc tccctgagga atttgggcgg cggcggcagc gactggtcgg ggacgtcgtc
     3301 gatctgatga tggagccagc tataccattc gggcggaatc cggctcggat cgttggcgcc
     3361 ctcgtaggag acgaagcggc ggtccccctt tttcgacgcg taatagacat tgccgctcga
     3421 atcgctgcct accttgcggc cgtggcggcg caggtgaagc gaggtcgtca gggtcgcccc
     3481 gtcccaccag gtgaagagct ttttgagtac gcccatggcc gcgcggacta gcgccgatgg
     3541 cgggcgccgc gcaagccatt cctccagtcg cccggcgtcc gcgacagggc cgggccgtgt
     3601 ccgaagacgg gactaaagct gaactgcgcc agattgacgt aattccccga taagcggagg
     3661 ataaatctta agagtcgatg aatcaggaat tgacgaatcg aaaaatccta actagtcgag
     3721 ccacttagca gagggacccg ccgtggcacg cagggtcgac ctttccagtg gggcatcggg
     3781 gcatgtcatg gaatttccgg cgaaatcgct gcgcgcctgc tgcgcgctcg ctttgctccc
     3841 cttcatcggg gccgcgccgt tgaccgtggc gcaggcccag gcctcgactc cgccgctcat
     3901 ctggcagcag gcgggcgcga tccggatcct ttgcctggtg acggcgcctt cgacgcgacg
     3961 cgacgagacc gacaagctcc aggaagagct ttgcaccatc gtccgctcga ttgtttcaga
     4021 cggctcaccg gtaccggtga gccaggtcgg cttcggcgat cccgcggtgc tcgagcccgg
     4081 cactgtcacc cttctcgtcc atgcctccct ccagcgggac ggcgaccagc gcctcgtcgc
     4141 gctcgacgtt cggcccttcc ggccgggagg cgaccataat gtcgtccttt tcggccccgc
     4201 gccgcgcgcc gttcgcattc cgggcaccgg agcgggcggc aacaaactcg aggcggcgat
     4261 cgccgccgct ctcgcggaga tcctgccctg gcggcaggat cgtaatcacc acaatatcaa
     4321 ccgccttaat taggggaggc aaatatgacg cagcaagtta ccaaccaggc tgaggaagac
     4381 accgactatc cggtcgtcga tatcaacgac atcatcgccc tttatgccaa cgtcgacggc
     4441 accggcggca cgatttcgaa caagccggtc tacacgctcg accaagtcat cgcgaacatg
     4501 aaccgtacgt cctaccccgg ctccggcatt ccgggtccgc agtggaatta cggcgaagat
     4561 ttcatgggcc agaacaagag cggcgacccc agcatcatca ccttcggctt ccagaccacc
     4621 acgtcggtcg cccaggcgcc ttatgccttt tacgacaatg ccggcaatct ggtggcggcc
     4681 cgctcgcaat attttggctt ccagcccttt tcggccgctc agatggacgc gacccgcaag
     4741 gcgatcggcc tgtgggacga tctcgtcgcc gtgagcttcg tcgaagtggc caatgtcgcc
     4801 aatgccgatc tcacctacgg caacgtcacc aaccagccca acacccaagc ctatgcttat
     4861 ctgccctata attacagcgg gcagtcgtcc ggcctcgagg gcgacgtctg ggtcggcgcg
     4921 tcggcgttga gcaacttcca gctcggcaac ggcttctacg gcctcgccac cctgatccac
     4981 gagaccggcc acgcgatcgg actccagcat ccgggcgcct acaatgcggc tccgggcctc
     5041 tcgatcacct tcccggtgaa cgcggaatat tatcaggaca cccgcatgta ttcgcagatg
     5101 tcctatttca acgccgagtt cagcggcggc gggcacatcg actggaaccg cctcaactgg
     5161 gtctacggcc agactccgct gctccatgac atcgccacca tccaggcgat ttacggcgcg
     5221 gatccgacga cccgagtcgg cgataccgtc tacggcttca acagcacggc cggtaaggag
     5281 gtctacgact tctcgaccaa caccatgccg gtcatctcga tctacgacgc cggcggcatc
     5341 gacacgctcg atttctcggg ctggaacacc aagtcgatca tcgacctcaa cgccggcgcc
     5401 ttctccgacg gcggcggcag cggagtcgtt ccgctcgacg tgctcaaggc ccgcggcctg
     5461 cttgctccga gctacaccga ggctcagtat acggcgctcc gcgcccgcta caacgcggtc
     5521 gacgggatgc tccaccagaa catctcgatc gcctacggca ccaccatcga gaacgcgacc
     5581 ggcggcggcg gcgatgatct gatcatcggc aacggcgtcg acaacgtcct catcggcaat
     5641 gccggcaacg acaacatcga aggccgtctc ggcaacgaca cgatcaacgg cggcgtcggt
     5701 ctcgacacca tgctcggcgg ggcgggcaac gacctctact tcgtggacaa cctcggcgac
     5761 acggtggtgg aggaagccgg aggcggcagc gaccgggtcg agagc
//
LOCUS       DASWNU010000533         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_229931, whole genome shotgun sequence.
ACCESSION   DASWNU010000533 DASWNU010000000
VERSION     DASWNU010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229931"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /gene="gcvT"
                     /locus_tag="VGB57_10520"
     CDS             <1..825
                     /gene="gcvT"
                     /locus_tag="VGB57_10520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0131826.1"
                     /translation="GIVDDLMATRRGDHFYVVVNGATKEGDIAYLRERLPRSVVLDHM
                     KEQALLALQGPKAVDVLSRLVPGVEQLGFMTGGAFTLDGAAAWISRSGYTGEDGFEIS
                     LPAGRAASVAELLAGQEEVKPIGLGARDSLRLEAGLPLYGHDLDSDTTPIEADLGFAI
                     SKRRRSEGGFAGWPRILREIEQGPVRRRVGLAVEGRQPVREGAAVIDSDGDEVGKVTS
                     GGFSPMLEAPIAMAYVPAGSVEPGTDIRLVQRGKVFQAKVAAMPFVPHRYHRKGAA"
     gene            825..1196
                     /gene="gcvH"
                     /locus_tag="VGB57_10525"
     CDS             825..1196
                     /gene="gcvH"
                     /locus_tag="VGB57_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY0131827.1"
                     /translation="MSVYYTEEHEWIRVDGDEATVGITDFAQGQLGDIVFVELPEAGR
                     QVVKGGEAAVVESVKAASDVYAPVDGEIVEPNPALGDDPSLVNSDPEGEGWFFRLRLA
                     DPSQLDGLMDAGAYKAFTESQ"
     gene            1193..1981
                     /locus_tag="VGB57_10530"
     CDS             1193..1981
                     /locus_tag="VGB57_10530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0131828.1"
                     /translation="MSLNPSSQWSPSDYATNAAFVPALGAAALDLLAPQPGELILDLG
                     CGDGVLTRRIMDTGALVIGLDASEAMVEAARGRGVDAFVADAQALDLDSQASRFGQFD
                     AAFSNAALHWMLDPDAVASGVFSVLKAGGRFAGEMGGEGNIATLRAGIRAELSERGYH
                     VPAEDPQWYPGCQEFVRLYACAGFTGIQASLIPRPTPLPAGVAAWVKTFRAGWLEVAG
                     VPEAARDDVAAAVEARLRPQLQRPDGSWVADYVRLRFTMRKPEE"
     gene            2193..>3017
                     /locus_tag="VGB57_10535"
     CDS             2193..>3017
                     /locus_tag="VGB57_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEY0131829.1"
                     /translation="MRYLPLADADRQEMLRVVGASSIDELFVDVPEAARLGDKIGGLP
                     DHLSEMAVERELARLSRKNLVAGDVPFFLGCGAYKHHIPASVDHLIQRGEFLTSYTPY
                     QPEIAQGTLQALFEFQTQVARLYGCDVANASMYDGSTACWEAIGMARRITRRGKAILS
                     SGLHPHYVSVAKTMARFTGDVLEAGVPELGPEPDTARLLDSIDEETSCVVVQYPDILG
                     RIGDLSALAERAHEKGALLVAVVTEPVALGLIRSPGEMGADIVVGEGQAIGVGLNFG"
BASE COUNT          453 a         1056 c         1026 g          482 t
ORIGIN      
        1 ggtatcgtcg acgacctgat ggccacccgc cgcggcgatc atttctatgt cgtcgtcaac
       61 ggcgccacca aagagggcga tatcgcctat ctcagggagc ggctgccgcg ctccgtcgtt
      121 ctcgaccata tgaaggagca ggccctgctc gccctccagg ggcccaaggc tgtcgacgtg
      181 ctctcgaggc ttgtccccgg agtcgagcag ctcggcttca tgaccggtgg cgccttcacc
      241 ctggatggag ccgccgcctg gatcagccgc tccggctata ccggcgagga cggcttcgaa
      301 atctcgcttc ccgccgggcg ggccgcttcg gtcgccgaac tcctcgccgg gcaggaggaa
      361 gtgaagccga tcggcctcgg cgcccgcgat tcgctccggc tcgaggcggg gctgccgctc
      421 tacggccacg atctcgactc cgacaccacc ccgatcgagg ccgatctcgg cttcgcgatc
      481 tcaaagcgcc gccgctccga aggcggcttc gccggctggc cgcggatcct gcgcgaaatc
      541 gagcagggac cggtccgccg ccgcgtaggc ctcgccgtcg agggccgcca gccggtgcgc
      601 gagggcgcgg ccgtgatcga ttccgacggc gacgaggtcg gcaaggtgac cagcggcggc
      661 ttctcgccga tgctggaggc gccgatcgca atggcctacg tcccggcggg ctcggtcgag
      721 ccgggcaccg acatccgcct cgtccagcgc ggcaaggtct tccaggcgaa ggtggcggcg
      781 atgcccttcg tcccccatcg ctatcaccgc aagggagccg cttgatgagc gtctattata
      841 ccgaggagca tgagtggatc cgggtcgacg gcgacgaggc gacggtcggg atcaccgatt
      901 tcgcccaggg ccagctcggc gacatcgtct tcgtcgagct gcccgaggct ggccgccagg
      961 tagtgaaggg cggcgaggcc gccgtggtcg agtcggtcaa ggcggcgtcc gacgtctatg
     1021 cgccggtcga cggcgagatc gtcgagccca atccagcgct tggcgacgat ccctccctgg
     1081 tcaacagcga cccggaaggc gaaggctggt tcttccggct gcgtctcgcc gacccctcgc
     1141 agctcgacgg actgatggac gccggcgcgt acaaagcctt cacggagagc cagtgagcct
     1201 caatccctcc tcccaatggt cgccgagcga ctatgcgacc aacgccgcct tcgtgccggc
     1261 gctcggcgcc gcggcgctcg atctgctggc gcctcagccg ggcgagctga tcctcgacct
     1321 cggctgcggg gacggcgtcc tcactcgcag gatcatggat acgggcgcgc tggtgatcgg
     1381 cctcgacgct tccgaagcga tggtcgaagc ggcccggggg cgcggagtcg acgcgttcgt
     1441 cgccgacgca caggcgctcg atctcgacag ccaggcgagc cgtttcggtc agttcgacgc
     1501 ggctttctcc aatgccgccc tgcactggat gctcgatccc gacgccgtcg cctcgggcgt
     1561 cttctcggtg ctcaaggccg gcggccggtt cgccggcgag atgggcgggg agggcaatat
     1621 cgcgaccctt cgcgccggca tccgcgcgga gctgagcgag cgcggctacc atgtgccggc
     1681 cgaggatccg caatggtatc ccggctgcca ggagttcgtc cggctctatg cctgtgccgg
     1741 cttcaccggg atccaggcca gcctcattcc gcggccgacg ccgcttccgg ccggggtcgc
     1801 cgcctgggtc aagaccttcc gagccggctg gctcgaagtc gcgggcgtgc cggaagccgc
     1861 gcgcgacgac gtcgcggcgg cggtcgaggc tcggctccgg ccgcagctcc agcggcccga
     1921 cggcagctgg gtcgccgatt acgtgcggct gcgcttcacg atgaggaagc cggaggaatg
     1981 accaagcact ttgcctcccc aagcgtggct tggggagggg gaccatcgaa agggtggtgg
     2041 aggggtatcg gccgcggatt caacgggtcc ggagcgagcc tcccagtcag cgccatctac
     2101 ccctccacca cccttcgggt ggtccccctc cccgcgagct tcgctcgcag ggaggcaaaa
     2161 gggccgccgt tccaactcag gaaaacccgc tgatgcgtta ccttcccctt gcagacgccg
     2221 accggcagga aatgctccgc gtcgtcggag cctcgtcgat cgacgagctg ttcgtggacg
     2281 tgcccgaggc cgcccggctc ggcgacaaga tcgggggact gcccgaccat ctcagcgaga
     2341 tggccgtgga gcgcgaactc gcccgcttgt ctcgcaagaa tctggtcgcc ggcgacgtgc
     2401 ccttcttcct cggctgcggc gcctacaagc accacatccc cgcctcggtc gaccatctca
     2461 tccagcgcgg cgagttcctg accagctaca cgccctacca gcccgagatc gcccagggca
     2521 cgctgcaggc cctgttcgag ttccagacgc aggtggcgcg gctttacggc tgcgacgtcg
     2581 ccaacgcctc catgtacgac ggctcgaccg cctgctggga agcgatcgga atggcccggc
     2641 ggatcacccg ccgaggcaag gcgattctct cctccggcct tcacccgcat tacgtttcgg
     2701 tggcgaagac gatggcccgc ttcaccggcg acgtgctcga ggcgggcgtt ccggaactgg
     2761 gaccggagcc cgacacggcg cgcctgctcg actcgatcga tgaggagacg agctgcgtcg
     2821 tcgtccaata tcccgatatc ctgggccgca tcggcgatct gtcggcgctg gccgagcggg
     2881 cgcatgagaa aggcgccctg ctggtggccg tcgtcaccga gccggtcgct ctgggcctga
     2941 tcaggtcgcc gggcgagatg ggcgctgaca tcgtcgtcgg cgaaggccag gcgatcggag
     3001 tcggcctcaa tttcggc
//
LOCUS       DASWNU010000534         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_151418, whole genome shotgun sequence.
ACCESSION   DASWNU010000534 DASWNU010000000
VERSION     DASWNU010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151418"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..943)
                     /gene="map"
                     /locus_tag="VGB57_10540"
     CDS             complement(119..943)
                     /gene="map"
                     /locus_tag="VGB57_10540"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0131830.1"
                     /translation="MTQYQAISGTELVGRSGAIKLHGPEGFEGMRRAGRAAAEVLDTL
                     VPHVVPGMTTQAIDDIVHREMIARGGIPATLGYRGFTGSCCTSINHVVCHGIPGDRTL
                     KDGDIVNIDVTIIVDGWHGDTSRMFLVGDVPIKARRLVDITYECMMLGIEQARPGKFL
                     GDIGHAIQTYAERNRYGVVRDFCGHGLGRLFHDAPEVVHAGRPGSGPELRPGMFFTIE
                     PMINIGKPAVKLLEDGWTAVTRDRSLSAQFEHSVGITEDGCEIFTKSPGGFDRPPY"
     gene            1160..>1845
                     /locus_tag="VGB57_10545"
     CDS             1160..>1845
                     /locus_tag="VGB57_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY0131831.1"
                     /translation="MTEERIWTAGLLIIGDEILSGRTQDRNVAQIAGWLNLQGIRLSE
                     VRVVPDVIERIASAVNALRAENDYLFTTGGIGPTHDDITVDAIAHALGVPVVVHPGAR
                     AILEDYYATRGGLNEGRLRMARVPEGAELIENRMSGAPGIRHGNLFILAGVPHIATQM
                     LESLAGKIEGGLPLLSRTIGCWTQESEVARLLAEVERAHEGCQIGSYPFFREGRVGAN
                     FVIRSTDEAAL"
BASE COUNT          305 a          597 c          635 g          308 t
ORIGIN      
        1 ccgacccccg cggccgctcc ttcaccttcg ccgccgaggt gaagttctaa gcgacggctc
       61 cgccttgagc tgaaacgaaa aggcccgggt gaacgcccgg gcctttttcg tttgcagcct
      121 aataaggcgg ccggtcgaag ccgccggggc tcttcgtaaa gatctcgcag ccgtcctcgg
      181 tgatcccgac gctgtgctcg aactgggcgg agagcgagcg gtcgcgggtg accgccgtcc
      241 acccgtcctc gagcagcttc accgccggct tgccgatgtt gatcatcggc tcgatcgtga
      301 agaacatgcc cggccgaagc tcggggccgc tgccgggacg gccggcatgg acgacttcgg
      361 gagcgtcgtg gaacagccgg ccgaggccgt ggccgcagaa gtcgcggacg actccgtagc
      421 ggtttctctc cgcataggtc tggatggcgt ggccgatgtc gccgaggaac ttgccgggcc
      481 gggcctgctc gatccccagc atcatgcact cgtaggtgat gtcgaccagc cggcgcgcct
      541 tgatcggaac gtcgccgacc aggaacatcc ggctggtgtc gccgtgccag ccgtcgacga
      601 tgatggtgac gtcgatgttg acgatgtcgc cgtccttcag ggtccggtcg cccggaatgc
      661 cgtggcagac gacatggttg atcgaggtgc agcagctgcc ggtgaagccg cgatagccga
      721 gcgtcgccgg aatcccgccg cgcgcgatca tctcgcgatg gacgatgtcg tcgatcgcct
      781 gggtggtcat gccggggacg acgtgcggga cgagcgtatc gagcacttcg gccgccgctc
      841 ggccggcgcg gcgcatgcct tcgaagcctt ccggtccatg gagcttgatc gccccgctgc
      901 ggccgaccaa ttcggtgccg ctgatcgcct gatattgggt catcggatgg atatagggga
      961 aggcggtgga ttttgcacgg ggggcattgc cgtcattccg gcgaaggccg gaatccatga
     1021 acaccgcgca gcgaggactc gacgatccgt gtccgtggat cccggccttc gccgggatga
     1081 cgaatcatcc gcgaggcggc tccgccccgt catgctgaaa caggttcagc atgacggggc
     1141 caggcgctaa agccgctgca tgaccgaaga acgcatctgg acggccggcc tcctcatcat
     1201 cggcgacgag atcctttccg gccgaaccca ggacaggaat gtcgcccaga tcgcgggctg
     1261 gctgaacctg cagggcatcc gtctgagcga ggtgcgggtg gtgccggacg tgatcgagcg
     1321 gatcgcctcg gcggtgaacg cgctgagggc cgagaacgac tatctcttca ccacgggcgg
     1381 gatcggaccc acgcatgacg acatcaccgt cgacgccatc gcccacgcgc tgggagtgcc
     1441 ggtcgtcgtc catcccgggg cacgggcgat actcgaagat tattacgcga ctcgcggcgg
     1501 cctcaacgaa gggcggctca ggatggcccg ggtgcccgag ggcgccgagc tgatcgagaa
     1561 ccggatgtcc ggcgcccccg gtatccgcca cggcaacctc ttcatcctgg ccggagtgcc
     1621 gcacatcgcg acccagatgc tggaatcgct ggcggggaag atcgagggcg gcctgcctct
     1681 tctctcccgg acgatcggct gctggaccca ggagagcgag gtggcgcggc tgctcgccga
     1741 ggtcgagcgc gcccatgaag gctgccagat cggctcctac cccttcttca gggagggcag
     1801 ggtcggcgcc aatttcgtca tccgctcgac ggacgaggcg gctct
//
LOCUS       DASWNU010000535         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_280528, whole genome shotgun sequence.
ACCESSION   DASWNU010000535 DASWNU010000000
VERSION     DASWNU010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280528"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1015
                     /gene="lipA"
                     /locus_tag="VGB57_10550"
     CDS             92..1015
                     /gene="lipA"
                     /locus_tag="VGB57_10550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0131832.1"
                     /translation="MSEALAPAPAPRQRKPDWIRVKAPTSVGFAETKKLMRSLNLATV
                     CEEAACPNIGECWTKKHATVMILGDTCTRACAFCNVKTGMPRAVDPLEPEHVATAAAE
                     LGLTHIVVTSVDRDDLPDGGARQFVRVIEALRRTTPSTTIEILTPDFRNKPESAVEAI
                     VAARPDVYNHNLETVPRLYPTIRPGARYYASLRLLESVKRQDPTIFTKSGVMVGLGEQ
                     RLEVHQVMDDMRSADVDFLTMGQYLQPTPRHARVEEFVTPAAFAAYGAIARAKGFLLV
                     AASPLTRSSYHAGDDFAKMKAAREARLRVGR"
     gene            1137..1601
                     /locus_tag="VGB57_10555"
     CDS             1137..1601
                     /locus_tag="VGB57_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY0131833.1"
                     /translation="MPRHTETRRMPYTPEQMFDLVADVKRYQEFLPWIAATRVRSDSE
                     TLMIADLVVGFRAIKETFTSRVEKQRPTRIHTEYIEGPLKYLQNSWVFKPDGQGGTEI
                     DFCVDFAFKNRLFESLAGQMFDRALRRMIGAFEDRARDLYGEGGADSKSVVS"
     gene            complement(1699..2232)
                     /locus_tag="VGB57_10560"
     CDS             complement(1699..2232)
                     /locus_tag="VGB57_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY0131834.1"
                     /translation="MSEAHDSILPPELIEAARKVVEASRAAGIRLAVAESCTGGLVAA
                     ALTEIPGSSDVLEAGFVTYSNESKIALLGVSEDMVETFGAVSIAVAWGMAQGALKRCA
                     ADTAVAVTGIAGPGGGSDKKPVGTVVFARARRHADPREIVADTKHFGDLGRGGVRLQA
                     ALCALELLMPVESKSEG"
     gene            complement(2229..>2437)
                     /locus_tag="VGB57_10565"
     CDS             complement(2229..>2437)
                     /locus_tag="VGB57_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131835.1"
                     /translation="TARSMLHDVAVAELFAKIKPEECAQFDRIAGLLSPLPPANLAGL
                     VAAAAAMGMKGDKKGPAICPAPLP"
BASE COUNT          404 a          847 c          806 g          380 t
ORIGIN      
        1 attgaagtcg gccattccat tctccccggt tcccgctagc ggcgcgaggg gaggggtggc
       61 aagagcggcc cgggatcgct atctggcccc catgtccgaa gcactcgctc ccgcgcccgc
      121 tcccaggcag cgcaagcccg actggatcag ggtgaaggcg cccaccagcg tcggcttcgc
      181 ggaaaccaag aagctgatgc gcagcctcaa cctcgccact gtctgcgagg aggcggcctg
      241 ccccaatatc ggcgagtgct ggaccaagaa gcacgccacg gtgatgatcc tcggcgacac
      301 ctgcacccgc gcctgcgcct tctgcaacgt caagaccggg atgccgcgcg cggtcgaccc
      361 gctcgagccc gagcatgtcg ccacggcggc ggcggagctg gggctgaccc atatcgtcgt
      421 cacctcggtc gaccgcgacg atctgcccga cggcggcgcc aggcagttcg tgcgcgtgat
      481 cgaggcgctg cgccggacca cgccgtccac caccatcgag atcctgacgc cggatttccg
      541 caacaagccg gaaagcgcgg tcgaggcgat cgtcgccgcc cggccggacg tctacaacca
      601 caatctcgag acggtgccga ggctctatcc caccatccgt ccgggggccc gctattacgc
      661 ctcgcttcgc ctgctcgaga gcgtgaagcg gcaggatccg accatcttca ccaagtcggg
      721 cgtgatggtc ggcctcggcg agcagcgcct cgaagtccat caggtgatgg acgacatgcg
      781 ctcggccgac gtcgatttcc tgaccatggg ccagtatctc cagccgaccc cgcgccatgc
      841 cagggtcgag gagttcgtca cccccgcggc cttcgccgcc tacggcgcca tcgcccgggc
      901 caagggcttc ctgctcgtcg ccgcctcgcc tctgacccgg tccagctacc atgccggaga
      961 cgatttcgcg aagatgaagg cggcgcgcga agccaggctg agagttgggc gttaatggaa
     1021 tgatggccgc gccccgcttc gtccttccct cgaaaagagg gacagtccct catttcggaa
     1081 tgacggcaaa aagaggcact gtccctcttt ttcgctcggt ggggtagggg tccgccatgc
     1141 cccgccacac cgagacccgg cgcatgccct acacgcccga gcagatgttc gatctggtcg
     1201 cggacgtgaa gcgttaccag gaattcctgc cctggatcgc cgcgacccgg gtgcgctcgg
     1261 acagcgagac cttgatgatc gcggacctcg tcgtcggctt ccgggcgatc aaggagacct
     1321 tcacctcgcg cgtcgagaag caacggccga cccggatcca caccgaatat atcgagggcc
     1381 cgctcaaata tctccagaac agctgggtgt tcaagccgga cgggcagggg ggaacggaga
     1441 tcgacttctg cgtcgacttc gccttcaaga accggctctt cgaaagcctg gccggccaga
     1501 tgttcgaccg ggcgctgagg cggatgatcg gggcgttcga ggacagggcc agggacttgt
     1561 atggagaggg cggggcggac tcgaaaagcg tggtgagctg accgttaccg tcatcccagc
     1621 gaaggctggg atcccagggg gtgaaaggcg ccggcggtct tcacccgccg gggccccagc
     1681 cttcgctggg gcgaggggtc agccttcaga cttgctctcc accggcatca acagctccag
     1741 cgcgcaaagc gccgcctgca gccgaacccc gccccggccg aggtcgccga aatgtttcgt
     1801 gtcggcgacg atctccctgg gatcggcgtg gcggcgggcg cgggcgaaga ccaccgtccc
     1861 gaccggcttc ttgtcgcttc cgccgccggg tcccgcgatc cccgtgacgg ccaccgcggt
     1921 gtccgcggcg cagcgcttca gggccccctg cgccatcccc caggcgacgg cgatcgacac
     1981 cgccccgaac gtctcgacca tgtcctcgct gactccgagc agggcgatct tggattcgtt
     2041 cgaataggtg acgaacccgg cctcgagcac gtccgacgag cccggtatct cggtcaaggc
     2101 ggcggcgacg aggccgccgg tgcagctctc cgccaccgcg agcctgatcc cggcggcacg
     2161 gctcgcctcg acgaccttgc gggccgcctc gatcagctcg ggcgggagga tggagtcgtg
     2221 cgcttcgctc acggcagcgg tgcagggcag attgcggggc ccttcttgtc gcccttcatg
     2281 cccattgccg cggcggccgc gaccagcccg gcgaggttgg cggggggcag cggagagagc
     2341 aggccggcga tccggtcgaa ctgggcgcat tcctccggct tgatcttggc gaagagctcg
     2401 gcgacggcga cgtcgtgaag catgcttcgg gccgtcc
//
LOCUS       DASWNU010000536         5109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_203088, whole genome shotgun sequence.
ACCESSION   DASWNU010000536 DASWNU010000000
VERSION     DASWNU010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203088"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VGB57_10570"
     CDS             <1..1177
                     /locus_tag="VGB57_10570"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEY0131836.1"
                     /translation="GEGEDAASAAGGARAVAAPEDNGHAIAAAPAEEAQAQQATVQAR
                     PKEGQEPASLKVEPEVPEGTETVRTTVREALRDAMAEEMRSDDRVFVMGEEVAEYQGA
                     YKVTQGLLDEFGPKRVIDTPITEYGFAGVGAGAAMGGLKPIIEFMTFNFAMQAIDHII
                     NSAAKTNYMSGGQMRCPIVFRGPNGAASRVGAQHSQNFGPWYANVPGLVVIAPYSAAD
                     AKGLLKAAIRSEDPVVFLENELLYGQSFDVPTLDDYVLPIGKARIAREGRDVTLVSYS
                     IGVGVALDAAAQLAEAGIEAEVIDLRTLRPLDKATVLASLKKTNRMVCVEEGWPVCSI
                     SAEIMATAMEEGFDDLDAPVLRVTDEDVPLPYAAKLEKMALIRVERVVEAAKKVCYR"
     gene            1271..1936
                     /locus_tag="VGB57_10575"
     CDS             1271..1936
                     /locus_tag="VGB57_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY0131837.1"
                     /translation="MDLDPDRTLALTYVPARRRAAVGALWRLDSALGAALAGGREPLI
                     SQIKLAWWREALERLDREPAPAEPVLRELAEHVLPASVGGAELAAMEEGWTILASPEA
                     LTRDQLATYARTRGGGLFRSTARLLGESQTTLEAALEGAGEAWALVDLARHCATSEDA
                     DIALAAARECGLPRHWPSRLRPLGMLAVLAARDLDPARPPWEARGAPARMWRMLRHRI
                     SGY"
     gene            2017..2457
                     /locus_tag="VGB57_10580"
     CDS             2017..2457
                     /locus_tag="VGB57_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0131838.1"
                     /translation="MVRYVAGGASALLLIAAGFFVWRSGAEAEEVVPPPPPPRSASYR
                     AGAGPRLSQPPAQSELSREARRFARADRDRDGRITLDEQLQPRRKAFARADRDGNGSL
                     SFEEWTAASAQKFAKADKDKSGWLDAAEYEASRPKPKARPKCKY"
     gene            2543..2908
                     /locus_tag="VGB57_10585"
     CDS             2543..2908
                     /locus_tag="VGB57_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEY0131839.1"
                     /translation="MARTVNWTDRNSAVLRARALRRAPTPPEFRLWLLLRQRPNGLKF
                     RKQHPLGPYTLDVYCPAAKLVGEVDGVSHERGDNPARDRRRDAWLHEQGLRVLRFDAS
                     DVMKDVESVVTAILLAARR"
     gene            complement(2909..4279)
                     /gene="trmFO"
                     /locus_tag="VGB57_10590"
     CDS             complement(2909..4279)
                     /gene="trmFO"
                     /locus_tag="VGB57_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEY0131840.1"
                     /translation="MTHDVHIIGGGLAGSEAAWQLAEAGVKVRLSEMRGSGESTPAHH
                     TDSLAELVCSNSFRSDDAEHNAVGLIHAEMRRLGSAIMAAADAHRVPAGSALAVDREG
                     FAAAVTGRIAAHPNIEIVRERVDTLPSSGQTIVATGPLTAMKLAESIGAVTGSDALAF
                     FDAIAPIVHRDSIDMDVAWMAARWDKGGKDYINCPMTKEQYLAFHQGLLDGEKTEFKE
                     WETDTPYFEGCMPIEVMAGRGLDTLRYGPMKPVGLDNPKTGHWPWAVVQLRQDNALGT
                     LWNMVGFQTKLKHAEQVRLFRTIPGLEKAEFARLGGLHRNTFINSPRLLDQTLRLKSA
                     PHIRFAGQITGCEGYVESAAIGLLAGRFAAAELRGQTLAPPPAETALGALLGHITGGA
                     DSATYQPMNVNFGLFPPVDGKARKADRKRAYTDRAKAALEQWIENLPPHREEMGRGTR
                     RSLVEG"
     gene            complement(4428..>5109)
                     /locus_tag="VGB57_10595"
     CDS             complement(4428..>5109)
                     /locus_tag="VGB57_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY0131841.1"
                     /translation="TRQGKAIRFAATEVREFQSRTATGVRGIRLQKDDEVISVSILTA
                     FDSASEEREAYLRAAPWKENENAPTLPPERMAEFEAAEEFILTVTENGYGKRTSSHDY
                     RRTGRGGQGITNIETSARNGCVVASFPASNGQQLMLVTDQAKLIRTTVGDIRIAGRNT
                     QGVTIFRVADNEHVVSAARIDEDEEPETEAEAMIAEELGEAPPAAAADTVQLDDGTGP
                     ETLPDSQE"
BASE COUNT          768 a         1730 c         1782 g          829 t
ORIGIN      
        1 cggcgaaggc gaggatgcgg cgtcggctgc gggcggcgca cgcgcggtcg cggcgcccga
       61 ggataacggc cacgcgatcg ccgccgctcc cgccgaggaa gcgcaggcgc agcaggcgac
      121 ggtgcaggcc cgcccgaagg aaggccagga gcccgccagc ctcaaggtcg agccggaggt
      181 gccggagggc accgagaccg tccgaaccac cgtccgggag gcgcttcgcg acgccatggc
      241 cgaggagatg cgctccgacg accgggtgtt cgtgatgggc gaggaggtcg ccgaatatca
      301 gggcgcctac aaggtcaccc agggcctgct cgacgagttc gggcccaagc gcgtgatcga
      361 caccccgatc accgaatatg gcttcgccgg agtgggcgcc ggcgccgcga tgggcgggct
      421 gaagccgatc atcgagttca tgactttcaa cttcgccatg caggcgatcg atcacatcat
      481 caattcggcg gcgaagacca attatatgtc gggcggccag atgcgctgcc cgatcgtctt
      541 ccgcggcccc aacggcgccg cctcgcgggt cggagcgcag cacagccaga atttcggacc
      601 ctggtacgcc aacgtccccg gcctcgtcgt catcgcccct tattcggccg ccgacgccaa
      661 ggggctgctc aaggcggcga tccgctccga ggatccggtc gtcttcctcg agaacgaatt
      721 gctctacggt cagagcttcg acgttccgac gctcgacgat tacgtcctcc cgatcggcaa
      781 ggcgcggatc gcccgcgagg gccgtgacgt caccctggtc agctattcga tcggagtcgg
      841 ggtcgccctc gacgcggcgg cgcagctcgc ggaagccggg atcgaggccg aggtgatcga
      901 ccttcgaacc cttcgcccgc tcgacaaggc gaccgtcctc gcctcgctca agaagaccaa
      961 ccggatggtc tgcgtcgagg aaggctggcc ggtctgctcc atctccgccg aaatcatggc
     1021 gacggcgatg gaggagggtt tcgacgatct cgacgcgccg gtcctccggg tcacggacga
     1081 ggacgtgcct ctgccttatg ccgccaagct cgagaagatg gccctgatcc gggtcgagcg
     1141 cgtcgtggag gcggcgaaga aggtgtgtta ccggtgagcc gccgtcatcc cggcgaaagc
     1201 cgggacctcg gggggtgaca ggctcggcgc ccattctcgc tggggtccgg ctttcgccgg
     1261 gatgacgtag atggatctcg acccggaccg caccctcgcg ctcacctacg tcccggcaag
     1321 acgccgcgcc gccgtcggcg ccttgtggcg gctcgattcg gcccttggag cggccctcgc
     1381 cggagggcgc gagccgctga tctcgcaaat caagctggcc tggtggcgcg aggccctgga
     1441 gaggctcgac cgcgagcctg ctccggccga gcccgtcctg cgggagctgg cggagcatgt
     1501 ccttcccgcc tcggtcggcg gagcggagct ggcggcgatg gaggagggct ggacgatcct
     1561 cgcaagcccc gaagcgctca cccgggatca gctggcgacc tatgcccgga cgcgcggcgg
     1621 cgggcttttc cgatctacgg cgcgcctgct cggcgaatcc caaaccaccc tggaggccgc
     1681 actggaggga gccggggagg cctgggccct ggtcgatctc gcgcgccact gcgccacttc
     1741 cgaagatgcc gacatcgccc tcgccgcggc ccgggaatgc ggtcttccgc gccactggcc
     1801 gtctcgatta aggccgctgg gaatgctggc ggtcctcgcc gcccgcgacc tcgacccggc
     1861 ccgccccccc tgggaagcgc gcggcgctcc ggcgcggatg tggcggatgc tccggcatag
     1921 aatttccgga tactgaacgc tttccataaa aaactatcga ctccagcccc gtttttcggt
     1981 gattttcctt cttccgatcc gtgagggagg agagacatgg tccgttacgt cgctggaggc
     2041 gcttcggcgc tgctgttgat cgccgccggc ttcttcgttt ggagaagcgg cgcggaggcg
     2101 gaggaggtgg tcccgccgcc gccgccgccg cggtccgcct cctaccgggc cggtgccggt
     2161 ccgaggctga gccagccccc ggcccagtcc gagctcagcc gcgaggcgcg gcgcttcgcc
     2221 cgcgccgacc gcgaccgcga cggccggatc accctcgacg agcagctcca gccccgtcgc
     2281 aaggccttcg cccgcgccga ccgcgacggc aacggcagcc tcagcttcga ggaatggacc
     2341 gcggcttcgg cgcaaaaatt cgccaaggcc gacaaggaca aatcgggctg gctcgacgcc
     2401 gcggagtatg aagcgagccg cccgaagccc aaggcgcggc cgaagtgcaa gtactgagaa
     2461 aatgggcgtg gcggcggaac ttctgcctcc ccgcggcgaa gccgcgggga gggggaccat
     2521 gcgaagcatg gtggaggggt agatggctcg gaccgtgaat tggaccgaca gaaactccgc
     2581 cgtccttcgg gcgcgggcac tcaggcgagc gccgacgccg ccggagttcc gcttgtggct
     2641 gctcctccgg caacggccca acggactgaa gttccgaaag cagcatccgc tcgggccgta
     2701 cacgctcgac gtctactgtc cggcggcgaa gctcgtcggt gaagtcgacg gggtcagcca
     2761 tgagaggggc gataatccgg ctcgggaccg gcgtcgcgat gcgtggctgc acgaacaggg
     2821 cttgcgcgtg cttcggttcg acgcttcgga cgtgatgaag gacgtcgagt cggtggttac
     2881 cgcgatcctc ctcgccgctc gacgctagct acccctccac caggcttcgc ctggtccccc
     2941 tccccatctc ttcgcgatgg ggaggcaagt tctcgatcca ctgctccaat gccgcctttg
     3001 cgcggtcggt ataggcccgc ttcctgtccg ccttccgcgc cttgccgtcg accggcggga
     3061 agaggccgaa attgacgttc atcggctggt aggtggccga gtccgcgccg ccggtgatgt
     3121 ggccgagcaa cgctcccagc gccgtctcgg ccggcggcgg cgccagcgtc tgcccccgaa
     3181 gctcggccgc ggcgaagcgg ccggccagaa ggccgatcgc ggcggattcg acatagcctt
     3241 cgcagccggt gatctggccg gcgaatcgga tgtgcggcgc gctcttcagc cgcagagtct
     3301 ggtcgagcag cctgggcgag ttgatgaagg tgttgcggtg cagtccgccg aggcgggcga
     3361 actccgcttt ctcaagcccg gggatggtcc ggaacagccg gacctgctcg gcatgcttca
     3421 gcttggtctg gaagccgacc atgttccaca aggtgccgag cgcattgtcc tggcggagct
     3481 ggacgaccgc ccagggccag tggcccgtct tcggattgtc gagccccacc ggcttcatcg
     3541 gcccgtagcg aagcgtgtcg agcccgcgcc cggccatcac ctcgatcggc atgcagccct
     3601 cgaaataggg cgtgtccgtc tcccattcct tgaactccgt cttctcgccg tcgagcaggc
     3661 cctggtgaaa ggccagatac tgctccttcg tcatcgggca gttgatgtaa tccttgccgc
     3721 ccttgtccca gcgcgccgcc atccaggcga cgtccatgtc gatcgagtcc ctgtggacga
     3781 tgggggcgat ggcatcgaag aaggcgaggg cgtcggaccc ggtgacggcg ccgatcgact
     3841 ccgccagctt catcgcggtg agcgggcccg tcgcgacgat ggtctggccg gaggagggga
     3901 gggtatcgac cctctcgcga acgatttcga tgttcgggtg cgctgcgatc cgcccggtga
     3961 cggccgcggc gaagccttcg cggtcgaccg ccagagccga tccggccggc acccggtggg
     4021 cgtcggccgc ggccatgatc gccgatccca gccggcgcat ctcggcatgg atcaggccga
     4081 cggcgttatg ctcggcatcg tcggagcgga agctgttcga gcagaccagc tcggcgaggc
     4141 tgtcggtatg atgggccgga gtagattcgc cggagccgcg catctcggac aggcgcacct
     4201 tcacccccgc ttcggccagc tgccaggcgg cttcggatcc ggcgagcccg ccgccgatga
     4261 tatggacgtc gtgggtcatg gcgtcgcgat aatcctcttg cgcgcgggga ggaagcacaa
     4321 gcccgtcgcc ccggcgaagg ccggggcccc cgggggtgaa aggctcggtg cacttcaccc
     4381 gctgggatcc cggccttcgc cgggatgacg gggaggtaga gtgcccttta ctcctggctg
     4441 tccggcaacg tttccggccc cgtcccgtcg tcgagctgaa ccgtgtccgc cgccgcggcc
     4501 gggggcgcct cgccgagctc ttcggcgatc atcgcctcgg cctcggtctc gggctcctcg
     4561 tcctcgtcga tccgggcggc ggagacgaca tgctcgttat cggcgacccg gaagatggtg
     4621 acgccctggg tgttgcggcc ggcgatcctg atgtcgccga cggtggtgcg gatgagcttg
     4681 gcctgatcgg tgacgagcat cagctgctgc ccgttgctgg ccgggaagct ggcgacgacg
     4741 cagccgtttc gcgccgaggt ctcgatgttg gtgatgccct ggccgccgcg gccggtgcgc
     4801 cggtaatcgt gcgaggaggt gcgcttgccg tagccgttct cggtgacggt gaggatgaac
     4861 tcctcggccg cttcgaactc ggccatccgc tccggcggca gcgtcggagc gttctcattc
     4921 tccttccagg gagcggcgcg cagataggcc tcgcgctcct ccgaagccga gtcgaaggcc
     4981 gtgaggatcg acaccgagat gacctcgtcg tccttctgga ggcggattcc gcgcactccg
     5041 gtggcggtgc gcgactggaa ctcgcgcacc tcggtcgcgg cgaagcgtat ggccttgccc
     5101 tgcctggtg
//
LOCUS       DASWNU010000537         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_513908, whole genome shotgun sequence.
ACCESSION   DASWNU010000537 DASWNU010000000
VERSION     DASWNU010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513908"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..777
                     /locus_tag="VGB57_10600"
     CDS             73..777
                     /locus_tag="VGB57_10600"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY0131842.1"
                     /translation="MTGRYGGMTPSFSRRSLVAAGLVLPLLTLPGCITRLGDVPDLEP
                     AIRRLLTLSSQRAFARLLTDEGFFRDDVARIQPPPQLGGRAVTAALAVALGTRAVQDR
                     LLRVVNQAAREGAERAAPIVYDSIRDMSIADALSIARGGPTAATDYLAQSMGERIFDA
                     IFPGVGEALRLAENGVIQRVLEFATGINFPGLQADVTRKTAAALYRAIGREESAIRAD
                     PAAAGDPVLAGLFGRR"
     gene            complement(811..2139)
                     /locus_tag="VGB57_10605"
     CDS             complement(811..2139)
                     /locus_tag="VGB57_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0131843.1"
                     /translation="MATSDERRDLNRAIWGIALPSMLTNVATALFGIADLWVIGQLGQ
                     AEPQAGVELGAKFMMGLLIVFNFLRTGTIALTAQAAGRGDEEAQAATLARAMGLALAI
                     GLVLLALKPVIVPWGMELLHARGGVAREAGVYVGIRYWGGLLWLANVALIGWLIGRRR
                     VRAVLWTEILSNLIHIALDLLFVLGFGWGVAGVAAATLLSEGGKTLALLAVTARQGPA
                     RAALASAGRRATWDSASLGALLRINRDLFLRTLLLTAAILLLTRQGAQQGPLVLAANG
                     ILYQLFILSALVLDGFESSAQVLCGEAVGSKDRRRFDALVRALLLWGLGGGALFSLAY
                     AAAGASFAATFSTDPAVAATTLSYVSWAILLPVLGVSSYVFDGIYIGATWTRAMLVTM
                     GAACAVYAALLYAAADMGNHGLWLAFSLFLVARAAAQAAMLPGLRRRTFE"
     gene            2311..>2787
                     /locus_tag="VGB57_10610"
     CDS             2311..>2787
                     /locus_tag="VGB57_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEY0131844.1"
                     /translation="MKFFVDTADTGEIRDLAETGLLDGVTTNPSLIHKAGRDFLEVVR
                     EICSIVPGPVSAEVVASDHKEMMREAEVLRGIADNIAVKVPLTLDGLKTCKALTGEGT
                     MVNVTLCFTANQALLAAKAGATFISPFVGRHDDTGFDGMQIISEIRTIYDNYDFETQ"
BASE COUNT          468 a         1039 c          894 g          386 t
ORIGIN      
        1 cagccttaga tacccctctc cccccggatc tctcccggaa agcggagacg agcaagtgat
       61 gcaacaaggc gcgtgacggg ccgttacgga ggcatgaccc cctcattctc ccgccgcagc
      121 ctcgtcgccg ccggtctcgt cctgccgctc ctgaccctgc ccggctgcat cacccgcctc
      181 ggcgacgtcc ccgatctcga gcccgcgatc aggcgcctgc tgaccctctc ctcacagcgg
      241 gccttcgccc ggcttctcac cgacgagggc ttcttccgcg acgacgtcgc ccggatccag
      301 cctccgcccc agctcggcgg aagggcggtg acggcggcgc tcgcggtggc gctcgggacg
      361 cgcgcggtcc aggaccggtt gctgcgggtc gtcaaccagg cggcacggga aggggcggag
      421 cgggcggcgc cgatcgtcta cgattcgatc cgcgacatgt cgatcgccga cgcgctctcc
      481 atcgcccgcg gcggcccgac cgccgccacc gattatctcg cgcaatcgat gggcgagcgc
      541 atcttcgacg ccatcttccc cggagtcggc gaggcgcttc gcctggccga gaacggcgtc
      601 atccagcggg tcctcgagtt cgcgaccggg atcaacttcc cgggcctgca ggcggacgtc
      661 acccgcaaga ccgccgccgc cctctaccgc gccatcggcc gcgaggaatc cgccatccgc
      721 gccgatccgg ccgcggcggg cgatcctgta ttggcgggcc tgttcggacg ccgctagtcc
      781 gtcatcccgg cttgcgccgg gatgacgagg ctattcgaac gtcctccgcc tcagccccgg
      841 aagcatcgcc gcctgcgccg ccgcccgcgc caccaggaac aggctgaagg cgagccagag
      901 gccgtgattg cccatgtcgg cggcggcgta cagcaaggcc gcatagacgg cgcaggccgc
      961 gcccatggtc acgagcatcg cccgggtcca ggtggcgccg atatagattc cgtcgaacac
     1021 gtaggagctg actcccagta ccggcagcag aatagcccag gaaacgtagc tgagagtcgt
     1081 cgccgccacg gccgggtccg tgctgaacgt cgcggcgaag ctcgcgccgg ccgccgcgta
     1141 ggcgaggctg aacagcgctc caccgcccag cccccacagc agaagcgccc tcaccagagc
     1201 gtcgaagcgg cggcggtcct tcgagcccac cgcctcgccg cacaatacct gcgccgagct
     1261 ttcgaagccg tcgaggacca gggccgagag gatgaacagc tggtagagga ttccgttcgc
     1321 ggcaagcacc agcggcccct gctgggcgcc ctgccgggta aggagcagga tggcggcggt
     1381 gaggaggagg gtgcgaagga agaggtcgcg gttgatccga agcagcgcgc ccaggctcgc
     1441 cgaatcccag gtcgcccgcc gcccggccga ggccagcgcc gcccgcgccg gcccctggcg
     1501 ggcggtcacg gccagcagtg cgagggtctt gccgccctcg gacagcaagg tggccgcggc
     1561 cacgcccgca acgccccagc cgaagccgag cacgaacagc aggtcgagcg cgatgtggat
     1621 gaggttggag aggatctcgg tccaaagcac cgcccgaacc cgccgccgcc cgatcagcca
     1681 gccgatcagg gcgacgttgg cgagccagag cagcccgccc caatagcgga tcccgacata
     1741 gacgcccgcc tcccgcgcca ctccgccccg ggcgtggagc agctccatcc cccagggcac
     1801 gatcaccggt ttcagcgcca gcaggaccag cccgatcgca agcgccagcc ccatcgcccg
     1861 ggccagggtc gccgcctgcg cttcctcgtc gccccggccc gccgcctggg cggtgagggc
     1921 gatcgtcccg gtcctcagga aattgaagac gatgagcagg cccatcatga acttggcgcc
     1981 cagctcgact ccggcctgcg gctcggcctg gccgagctgg ccgatcaccc acagatcggc
     2041 gatcccgaac agcgccgtcg cgacgttggt cagcatcgag ggcaaggcga tcccccagat
     2101 cgcccggttc aggtcccgcc gctcatccga ggtcgccaag cctgtccccg cccgtcattg
     2161 cgaggagcga agcgacgcgg caatccagct gcggcgtttg ccggaggctc cgcgctggat
     2221 tgcttcgcgt tgctcgcaat gacggtgttt cactatcggt tgaacctgcc ctagaaggcg
     2281 gccgaaaccc agccccaagg accccgtcat atgaaattct tcgtcgacac cgccgacacc
     2341 ggcgaaatcc gcgacctggc ggaaaccggc ctgctcgacg gagtcaccac caacccgtcc
     2401 ctcatccaca aggcgggccg cgatttcctc gaggtggtgc gcgagatctg ctcgatcgtg
     2461 cccgggccgg tctcggcgga ggtcgtcgct tccgaccata aggagatgat gcgcgaggcc
     2521 gaggtgctgc gcgggatcgc cgacaatatc gcggtcaagg tcccgctcac cctcgacggc
     2581 ctcaagacct gcaaggcgct gaccggcgag gggacgatgg tcaacgtcac cttgtgcttc
     2641 accgccaacc aggcgcttct cgccgccaag gccggcgcca ctttcatctc gcccttcgtc
     2701 ggccgccacg acgacaccgg cttcgacggg atgcagatca tctccgagat tcgcaccatc
     2761 tacgacaatt acgatttcga gacccag
//
LOCUS       DASWNU010000538         8135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_254904, whole genome shotgun sequence.
ACCESSION   DASWNU010000538 DASWNU010000000
VERSION     DASWNU010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254904"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /gene="pyk"
                     /locus_tag="VGB57_10615"
     CDS             <1..959
                     /gene="pyk"
                     /locus_tag="VGB57_10615"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0131845.1"
                     /translation="AALTDKDRADLVFALDQHVDWIALSFVQRPEDVAEARRLIAGRA
                     ALLAKIEKPAAVERLDGILELADAVMVARGDLGVELPPQAVPPLQKMIVEAARRMGRP
                     VVVATQMLESMISSPSPTRAEVSDVATAVYDGADAVMLSAESASGQYPCEAVTMMNSI
                     ATSVEADPTHAARVHFTQTLPDPTTADAIAQAANSMVETVGAAAIVCFTSSGSTARRL
                     SRERPAVPLLVLTPQLRTARKLGILWGAHGVRTRDIANFEEMIAKAKRMALRHGVARG
                     GDRIVIIAGVPFGTPGSTNVIHIVRLVGDELERHRKAQEKLR"
     gene            complement(1105..2607)
                     /locus_tag="VGB57_10620"
     CDS             complement(1105..2607)
                     /locus_tag="VGB57_10620"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY0131846.1"
                     /translation="MTAAPDVIERAYSLLHSGRAADALEISTRLAAAADAGHDALVLH
                     SAALKRIHRHEEALAVDERAARAFPRSGTAWHNLATTLGELGRGREALEVLARGFAVG
                     LDSPLSWQVKARAHVACGDLAAAESAYREASARAPDLAPLAAETADFLWMAGGDLAAA
                     QAPLDRAFHAGAPPAPLLLAKARLLASAGDRAQAAALLDRAAGHLPDEPQLAVAAAQA
                     ALEADRLADAERHMRRAEALAPGDPATLVQSTIISLALGRTEAALLSVGKARELAPLD
                     QSVLAWTATAARAAGDPLYRELCDYESMVGVYDLEPPAGWPTRTDFLRDLTAALDRHH
                     VMAQHPSAQSLRHGTQTTYRLSGSGEPAVQAFFKALDEPLREHLARLGTGGDPLRRRN
                     EGGYRIVGAWSVRLRQGGFHLDHMHHEGWISSAFYVETPGGALDRDGREGWLRLGQPP
                     FPTVPELPAERFVRPMPGRLVLFPSYMWHGTVPFHTEERRTTIAFDLLPG"
     gene            complement(2728..3621)
                     /locus_tag="VGB57_10625"
     CDS             complement(2728..3621)
                     /locus_tag="VGB57_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0131847.1"
                     /translation="MSDFEILDRDLLRRFPLPDHPDDGDKEDRGRLLVVAGSRELAGA
                     ALLAGTGGLRAGAGKLQIATGASVAPALAVAMPEARVVGCPETDEGCIAAEAIRPLLE
                     WAAGAQAILIGCGLQHGPPLEAMLDSLLESGVDKPLVLDAAVLGSLAPRAATLRAWRG
                     GAILLPHAREMARLLECEVEEVEADPLAAARRAAETFGAVSLIKGQYSFIVAPDGRAF
                     RFTGGGVGLATSGSGDALAGIVGGLAARGADPLTAALWGVYLHGESGRALAKRIGRIG
                     FLARELLGEVPGLMEGSSSRA"
     gene            complement(3618..4382)
                     /locus_tag="VGB57_10630"
     CDS             complement(3618..4382)
                     /locus_tag="VGB57_10630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0131848.1"
                     /translation="MRQRWPAKLWIVRHGQSAGNVARDAADAAGLGVIDLDIRDVDVP
                     LSDLGQRQADALGHWFAALPEEERPEVVLTSPYVRARQTAEAICKAGGSVADAKAPRQ
                     DERLREREFGLWDRLTTAGVREKFPEQAEQRTLLGKFYHRPPGGESWADVILRLRSAL
                     DTVSLHHADRRVLIVCHQVVVLCFRYILEELDEAQILAIDKEGDVVNCGLLQYEFEPD
                     DEKDCVPRLVCYNFAAPLIEEDAPITSEPDAMVAAR"
     gene            complement(4422..5036)
                     /locus_tag="VGB57_10635"
     CDS             complement(4422..5036)
                     /locus_tag="VGB57_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0131849.1"
                     /translation="MTAPPTAVIFDVGNVLYGWDPHSFLERQLPEDEARLRFIEEVDL
                     WGWHDSLDGGRPFDEAAAELSEKFPDYAAYISAWGERFGETISDPVPGVHAIVDELDR
                     RGAPLFAITNFSADFWAPFRAREDAFFRRFRGIVVSGEEKLLKPDPAIYYRALHRFGL
                     QAHEALFVDDRKINVEGAEAVGMQAHLFTGAEGLRRRLEAEGLL"
     gene            complement(5137..5262)
                     /gene="ykgO"
                     /locus_tag="VGB57_10640"
     CDS             complement(5137..5262)
                     /gene="ykgO"
                     /locus_tag="VGB57_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEY0131850.1"
                     /translation="MKIRNSLKSLKSRHRDCRVIRRRGRTYVINKTNRRFKARQG"
     gene            complement(5379..6557)
                     /locus_tag="VGB57_10645"
     CDS             complement(5379..6557)
                     /locus_tag="VGB57_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0131851.1"
                     /translation="MNVEEAEAIVKGVIGPLPLESFFNAVGRTVLDVPGGPDHERAGL
                     FGDDPVRTALSAYSTHAERLDCHGVNPPLPPPGPGKASDAAEFGKLIHAYHERDYTVR
                     MPEVLPLSPPLQRFARALELLLGQPVDASLFWSKAGAGAIVHYDNRDNLVFQLHGRKR
                     WYVSTEPPGLQNNWKHIGEPLAQLRRHKVIDVGPGDLLYIPRGTPHTVDSVSESLHLA
                     VLFVPTTLRDVLIAAIDQMSDEDRAWRETAVERSDLGRDGLSSMAAAGLERLIARCRS
                     GGFVEAAMEHRSSRFVGDLPPLPPPQAAPNVSAETRVRHAPLAVFHLRRAGDWIDLGF
                     PGDRLAIHPGVEEELRFIRDTAEFRIRDIPGASSVEVRMALVSRLIAAGLLEPAAAGP
                     "
     gene            complement(6696..7697)
                     /gene="galE"
                     /locus_tag="VGB57_10650"
     CDS             complement(6696..7697)
                     /gene="galE"
                     /locus_tag="VGB57_10650"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0131852.1"
                     /translation="MNKPNVLVTGGAGYIGSHAVLALLDSGWKVVVIDNLTTGFRWAV
                     PDPVAFVEGDIGDQALVARLIEEHDIGAIIHFAGSIVVPESVEQPLKYYENNTVKSRS
                     LIESAVKGGVRHFIFSSTAATYGIPEAVPVVETSRTQPINPYGWSKLMTERMLADTAF
                     AHKINFCALRYFNVAGADPQGRSGQSTAGATHLIKVAVEAATGKRQAVSVYGTDYDTP
                     DGTGVRDYIHVSDLAAAHVDALGKLVEDPTTSHIMNCGYGRGFSVLEVLDSVDRVTNM
                     RIERRMEPRRAGDPDSLVADNGRILATLPWRPRLDDLDTIVAHALAWERKLAERAGA"
     gene            7795..>8135
                     /locus_tag="VGB57_10655"
     CDS             7795..>8135
                     /locus_tag="VGB57_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131853.1"
                     /translation="MKTAFLRSAPLLLAAALAACAGGGGKERPTAGGADVARFHLGEP
                     VARAQIAIEPFDRADGNRPEFPAYAAAVGRQLSRLGWTVVPTTTGSEQIALIDLEQGS
                     REAIAALSAAR"
BASE COUNT         1258 a         2717 c         2953 g         1207 t
ORIGIN      
        1 tcgccgcgct gaccgacaag gaccgggccg acctcgtctt cgccctcgac cagcatgtcg
       61 actggatcgc cttgtcgttc gtccagcggc ccgaggacgt cgccgaggcc cggcggctga
      121 tcgcgggccg ggcggccctc ctcgccaaga tcgagaagcc ggccgcggtc gagcggctcg
      181 acggcatatt ggagcttgcc gacgcggtga tggtggcgcg cggcgacctc ggggtcgagc
      241 ttccgccgca ggccgtgccg ccgctccaga agatgatcgt cgaggcggcg cggcggatgg
      301 ggcggccggt ggtggtcgcg acccagatgc tcgaatcgat gatcagctcg ccgagcccga
      361 cccgggccga agtctccgac gtcgccaccg cggtctatga cggcgccgac gcggtgatgc
      421 tgtcggcgga gagcgcctcg ggccaatatc catgcgaggc ggtgacgatg atgaactcga
      481 tcgccaccag cgtcgaggcg gacccgaccc acgccgcgcg ggtccatttt acccagaccc
      541 ttccggatcc gaccaccgcc gacgccatcg cgcaggcggc gaactcgatg gtggagacgg
      601 tgggcgcggc ggcgatcgtc tgcttcacaa gctccggctc gacggcccga aggctgtcgc
      661 gcgagcggcc ggcggtgccg ttgctggtgc tcaccccgca gctgaggacg gcgcgcaagc
      721 tcggcatcct gtggggcgcg cacggggtca ggacccgcga catcgccaat ttcgaggaga
      781 tgatcgccaa ggccaagcga atggcgctcc gccacggggt ggccaggggc ggcgaccgga
      841 tcgtcatcat cgccggagtg ccgttcggga ccccgggatc gaccaacgtc atccacatcg
      901 tgaggctggt cggcgacgag ctcgagcggc accggaaagc gcaggagaaa ttgcgctagg
      961 cgcgtgatcc tcccgatcgg agatggggag gggaccaccc gaagggtggt ggaggggtaa
     1021 ttgctcaggc ctcgtcagct acccctccac caggcttcgc ttggtccccc tcccatcctg
     1081 cggatgggga ggattccagg tccctcaccc cggaagcaga tcgaaggcga tcgtggtgcg
     1141 ccgttcctcc gtgtggaacg ggacggttcc gtgccacatg taggacggga acaggaccag
     1201 cctcccgggc atgggacgga cgaagcgttc cgccggcagc tccggcaccg tcgggaaggg
     1261 cggctgcccg aggcggagcc agccttcccg cccgtcccga tcgagggcgc cgccgggagt
     1321 ctccacgtag aaggccgaag agatccagcc ctcgtggtgc atgtgatcga ggtggaatcc
     1381 gccctgccgg agccggaccg accaggctcc gacgatccgg tagccgcctt cgttgcgccg
     1441 gcgcagcgga tcgcctccgg tgccgagcct ggcgagatgc tcgcgcaggg gttcgtccag
     1501 ggccttgaag aaggcctgga ccgccggttc tccagatccc gagaggcgat aggtggtctg
     1561 ggtgccgtgg cgaagcgatt gcgccgacgg gtgctgcgcc atgacgtggt ggcggtccag
     1621 cgcggccgtc aggtcgcgga ggaagtccgt tcgggtcggc catccggcgg gcggctcaag
     1681 gtcgtagacc ccgaccatgg actcatagtc gcaaagctcg cggtagagcg ggtcgccggc
     1741 cgcccgcgcc gccgtggcgg tccaggcgag caccgactgg tcgaggggcg ccagctcgcg
     1801 tgccttgccg acgctgagca gggccgcttc ggtccggccg agcgccaggc tgatgatggt
     1861 cgactggacg agcgtcgccg gatcgccggg ggccagggcc tcggcgcggc gcatgtggcg
     1921 ctcggcgtcg gcgagccggt ccgcctccag cgccgcctgg gcggccgcga cggccagctg
     1981 gggctcatcc ggcagatgcc ccgccgcccg gtcgagcaag gcggcggcct gcgcccggtc
     2041 gcccgccgag gcgagcagcc tggccttggc gagcagcagt ggagcgggcg gcgctccggc
     2101 gtggaaggcg cggtcgagcg gcgcctgggc cgcggccaga tcgcctccgg ccatccacag
     2161 gaaatcggcg gtctcggcgg ccagcggcgc gaggtccggc gcccgcgccg acgcttcgcg
     2221 ataggcgctt tcggccgccg ccagatcgcc gcaggcgacg tgcgcgcgcg ctttcacctg
     2281 ccagctgagc ggcgaatcga gccccacggc aaagcccctg gcgagcacct ccagggcctc
     2341 acggcctcgc ccgagctcgc cgagggtcgt ggcgagattg tgccaggcgg ttccgcttcg
     2401 agggaaagcc ctggccgcgc gctcgtcgac ggcaagcgct tcctcgtgcc gatggatccg
     2461 cttgagggcg gcggaatgaa ggaccagggc atcgtggccg gcgtcggccg ccgcggcgag
     2521 gcgggtgctg atctcgagcg catccgcagc gcgccccgag tggaggagcg aataagcgcg
     2581 ttcgatgacg tccggggccg ctgtcacctt cgcggaatgc gggagggcgg aggccggatc
     2641 aagcccggac ccgtcattcc ggcgcaagcc ggaatccatg aacacggcgg ttcgggttcg
     2701 ggcaggacgg tgttcatgga tcccgggtca agcccgggat gacgaacctt ccatcagccc
     2761 cggcacttcg cccagcagct cgcgggcgag gaagccgatc cggccgatgc gcttcgcgag
     2821 cgctcggccc gactcgccgt ggaggtagac tccccacagg gcggcggtca gcgggtcggc
     2881 gccccgggcc gcgaggccgc cgacgatccc ggcgagcgcg tcgcccgagc cggaggtggc
     2941 gaggccgact ccgccgccgg tgaagcggaa ggcgcggccg tcgggggcga cgatgaagct
     3001 gtactggccc ttgatcagcg acaccgctcc gaacgtctcc gcggcccgtc gggcggcggc
     3061 gagcggatcc gcctccacct cctccacctc gcattcgagc agccgggcca tttcgcgggc
     3121 atgggggagg aggatggcgc cgccccgcca cgcccgcaac gtcgcggccc tcggagcgag
     3181 gcttccgagc accgcggcgt cgagcaccag cggcttgtcg acgccgctct ccagcagcga
     3241 atcgagcatg gcttcgagcg gggggccgtg ctggaggccg cagccgatca ggatcgcctg
     3301 ggctccggcg gcccattcga gcaggggccg gatcgcttcg gcggcgatgc agccctcgtc
     3361 cgtttccggg cagccgacga cccgagcctc gggcatggcc acggcgagcg ccggtgcgac
     3421 gctggcgccg gtggcgatct ggagcttgcc ggcgccggcc cggagcccgc ccgttccggc
     3481 gagcaaggcg gctccggcaa gctcgcggct tccggcgacg acgagcagcc ggccgcgatc
     3541 ctccttgtcg ccgtcgtccg ggtgatcggg aagcggaaag cggcggagca ggtctcggtc
     3601 gaggatttcg aaatcgctca tcgcgcggcg accatggcgt cgggctcgga cgtgatcggc
     3661 gcgtcctcct cgatcagcgg cgccgcgaaa ttgtagcaga ccagcctggg cacgcagtcc
     3721 ttttcgtcgt cgggctcgaa ttcgtattgg aggaggccgc aattgacgac gtcgccttcc
     3781 ttgtcgatcg ccaggatctg cgcctcgtcc agctcctcca atatgtagcg gaagcagagc
     3841 acgacgacct ggtggcagac gatcagcacc cggcggtcgg catggtggag gctgaccgtg
     3901 tcgagcgcgc tcctcaggcg aaggatgacg tcggcccagc tctcgccccc gggcggccgg
     3961 tggtagaatt tgccgagcag ggtgcgctgc tcggcctgct cggggaattt ctcgcgcact
     4021 ccggcggtag tgaggcggtc ccacaggccg aactcgcgct cgcgaaggcg ctcgtcctgg
     4081 cgcggggcct tggcgtccgc gacggagccg cccgccttgc agatcgcctc ggcggtctgc
     4141 cgggcgcgga cataaggcga agtcagcacc acttcgggcc gctcctcctc gggcaaggcg
     4201 gcgaaccagt ggccgagggc gtcggcctga cgctggccga ggtcgctgag cggaacgtcg
     4261 acgtcgcgga tgtcgaggtc gatcactccg aggccggcgg cgtccgccgc gtcgcgcgcg
     4321 acgttgcccg cgctctgccc gtgacggacg atccagagtt tggcgggcca tctttgtctc
     4381 atcgccgaaa caaagctcga gggcagcgga agttgcgtcg cctagagaag cccctccgcc
     4441 tccagccgcc gacgcaaacc ctcggcgccg gtgaacagat gcgcttgcat gccgaccgcc
     4501 tcggcgccct cgacgttgat cttgcggtcg tcgacgaaca gggcctcgtg cgcctgcagg
     4561 ccgaagcggt gaagggcgcg atagtagatg gcgggatcgg gcttgagcaa tttctcctcg
     4621 cccgagacga cgattccccg gaagcggcgg aagaaggcgt cctcgcgggc ccggaagggg
     4681 gcccagaaat cggcggagaa attggtgatg gcgaagagcg gcgcgccgcg ccgatccagt
     4741 tcgtcgacga tcgcgtggac tccggggacg gggtcgctga tcgtctcgcc gaatctttcg
     4801 ccccaggcgg agatgtaggc ggcgtaatcg gggaatttct cggaaagctc ggccgcggcc
     4861 tcgtcgaacg ggcggccgcc gtcgagactg tcgtgccagc cccacaggtc gacctcctcg
     4921 atgaagcgaa ggcgcgcctc gtcctcgggc aattgccgct cgaggaagga atggggatcc
     4981 cagccgtaga ggacgttgcc gacgtcgaag attacggccg tgggcggggc ggtcatgcgt
     5041 gaacgtcgcc ccagcgaagg ctggggcccc ggggggtgaa aggctcggtg cccattctcg
     5101 ctgggatccc agccttcgct gggatgacgg ccaggatcag ccctggcgcg ccttgaagcg
     5161 gcggttggtc ttgttgatga cgtaggtgcg gccccggcgg cggatcacgc ggcagtcgcg
     5221 gtggcgcgac ttgagggact tcagggaatt gcggatcttc atggcctgga acgcctgcct
     5281 gtctcgtcga aaatgtctgc tggaaaagcg aggcgctcac ctagggggaa ggggctttca
     5341 agtcaagctt gcgagggccg ccccggccgc cctcccgttc agggtcccgc cgccgccggc
     5401 tcgaggagcc cggcagcgat cagacgactg accagggcca tccggacctc gaccgacgag
     5461 gcgcccggaa tgtcccggat ccggaactcc gccgtgtccc ggatgaaccg cagctcctcc
     5521 tccacgccgg ggtggatcgc gaggcggtcc ccgggaaagc cgaggtcgat ccagtcccct
     5581 gcccgtctca gatggaagac ggcgagcggc gcatggcgga cccgggtctc ggcggagacg
     5641 ttcggcgccg cctgcggcgg tggaagcggc ggcaggtcgc cgacgaaccg cgacgatcga
     5701 tgctccatcg ccgcctcgac gaagccgccg gagcggcacc gggcgatcag ccgctccagg
     5761 ccggccgccg ccatcgacga cagtccgtcc cggccgaggt cgcttcgctc caccgccgtc
     5821 tcgcgccagg cgcggtcctc gtccgacatt tgatctatcg ccgcgatcag gacgtcgcgc
     5881 agggtggtcg gaacgaacag caccgcgagg tggagcgatt cggaaaccga atccaccgtg
     5941 tgcggggtgc cccggggaat gtagagaagg tcgcccgggc cgacgtcgat caccttgtgc
     6001 cgccgcagct gggccagcgg ctcgccgatg tgcttccagt tgttctgcag cccgggcggc
     6061 tcggtcgaga cgtaccagcg cttccggccg tgaagctgga agaccagatt gtcgcgattg
     6121 tcgtaatgga cgatcgctcc ggcgccggcc ttgctccaga acagggaagc atcgaccggc
     6181 tggccgagga ggagctcgag ggcccgggcg aaacgttgca gcggcggcga cagcggaagc
     6241 acctcgggca tgcggacggt ataatcgcgc tcgtgatagg cgtggatcag cttcccgaac
     6301 tcggccgcgt ccgaggcctt ccccggtccc ggcggcggca ggggcggatt gactccgtgg
     6361 caatcgagcc gctcggcatg ggtggaatag gcgctgagcg ccgtccggac gggatcgtcg
     6421 ccgaacaggc ctgcccgttc gtgatcgggg ccgcccggga catcgaggac ggtccgaccc
     6481 accgcattga agaacgactc gagcggcagc ggcccgatca cgcccttgac gatggcctct
     6541 gcttcttcaa cgttcatgtt tccgccctgg gcattcgaca gggcttacgg tcctaaagcc
     6601 agtaattcgt cattgcgagg agcgaagcga cgaagcaatc catcggacgt cggaggccgt
     6661 gctggattgc ttcgcttcgc tcgcaatgac gtgggctatg cccccgcgcg ctcggcgagc
     6721 ttgcgctccc aggccagcgc gtgggcgacg atcgtgtcga ggtcgtcgag cctcggccgc
     6781 cacggcaggg tggccaggat ccggccgttg tcggccacca gcgaatcggg atcgcccgcc
     6841 cgccgaggct ccatccgccg ctcgatccgc atgttggtga cccggtcgac cgagtcgagc
     6901 acctcgagca cggaaaagcc gcggccgtag ccgcaattca tgatgtggct ggtggtcggg
     6961 tcctcgacca gtttgccgag cgcgtcgaca tgggcggcgg cgaggtcgct gacgtggata
     7021 tagtcgcgca ctccggtgcc gtcgggggta tcgtaatcgg tgccgtagac cgagacggcc
     7081 tgccgcttgc ccgtcgccgc ctcgaccgcg accttgatga ggtgggtggc ccccgccgtg
     7141 gactggcccg aacggccctg cggatcggcg ccggcgacgt tgaagtaacg aagggcgcag
     7201 aagttgatct tatgtgcgaa ggcggtgtcg gccagcatcc gctcggtcat cagcttcgac
     7261 cagccataag ggttgatcgg ctgggtacgg gaggtctcga cgaccggcac ggcctcggga
     7321 atgccgtagg tcgccgctgt cgaggagaag atgaagtggc ggactccgcc cttcaccgcg
     7381 ctctcgatca gcgagcggct cttcaccgtg ttgttctcat aatatttgag cggctgctcg
     7441 acggattcgg ggaccacgat cgatccggcg aaatggatga tcgcgccgat atcatgctcc
     7501 tcgatcaggc gggcgaccag cgcctggtcg ccgatatcgc cctcgacgaa agcaaccggg
     7561 tccgggacgg cccagcggaa gccggtcgtg aggttgtcga tcaccaccac cttccagccc
     7621 gagtcgagca gcgccagcac ggcatggctg ccgatatagc cggcgccgcc ggtcacgagc
     7681 acattgggct tgttcatcta acctctttcc tgacggaacc tgattgcggc atgagcgctg
     7741 agaggatcgt tacagcgccc acatgaacgc gcgcgctaga caagaggtgc cgggatgaaa
     7801 acagcttttc tgcgcagcgc gcccctcctg ctggcggcgg cgcttgccgc ctgtgccggc
     7861 ggcggcggca aggagaggcc gaccgccggc ggagccgacg tcgcccggtt ccacctcggc
     7921 gaaccggtcg cgcgcgccca gatcgcgatc gagcccttcg acagggccga cggcaaccgg
     7981 ccggaattcc cggcctatgc cgcggcggta ggacgccagc tgagccgtct cggctggacg
     8041 gtggtgccga cgacgaccgg gtccgagcag atcgccctca tcgatctcga gcagggcagc
     8101 cgagaggcga tcgcggcgct gagcgcggcg cgtat
//
LOCUS       DASWNU010000539         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_125250, whole genome shotgun sequence.
ACCESSION   DASWNU010000539 DASWNU010000000
VERSION     DASWNU010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125250"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGB57_10660"
     CDS             <1..552
                     /locus_tag="VGB57_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0131854.1"
                     /translation="PAMAKLIPFGGKAPSVDPSAFVAPGAVLIGDIEIGPEASIWYNC
                     VLRGDVNRIRIGARTNIQDGSVLHVDSPRPGSPAGLPTIIGEDVLIGHMAMVHGCLLH
                     DRAFVGLGAIVMDGCEIEGGAMLAAGALLTPGRRIPAGQLWAGRPAKYVRDLNEAEIT
                     GQQAGVAHYVALARLHSAAIRGG"
     gene            complement(539..1048)
                     /locus_tag="VGB57_10665"
     CDS             complement(539..1048)
                     /locus_tag="VGB57_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131855.1"
                     /translation="MASLRPPLAILLPLLAAAGCAAPGPYPSLAPRPAEKAYADDAEE
                     RKPTPQPDDPALAAEIDRLLDAGRAGAAEFDSALPAAQAAAGRAGAAGSDAWIEAQQA
                     LSRLETARARTTAAVADLDRLAVERTSAGTLGSGDSERLRRAAAELQALTDAQADRLQ
                     RLGESLIRL"
     gene            1151..1227
                     /locus_tag="VGB57_10670"
     tRNA            1151..1227
                     /locus_tag="VGB57_10670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1185..1187,aa:Arg,seq:acg)
     gene            complement(1311..1955)
                     /locus_tag="VGB57_10675"
     CDS             complement(1311..1955)
                     /locus_tag="VGB57_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131856.1"
                     /translation="MLPRALLLLVLASLAPCGSPARGAEPGESRTFDLGPDGDRDRIR
                     ITIPVQYLKRQPDLRDFVSSLPALTVDLADFAPAPASRRGDRATGYLRVGWTREGSIA
                     KRTNGTWLNHYSTYSEAATDLFGLKRRLTTHRRFGDNSFLLIEPPGATMIVCEQLVSH
                     VPMVCTMATEYEKGLLVELSFDPFHLPNWKQLRRDAETFVRPKVTFGEQTPLGW"
BASE COUNT          298 a          702 c          689 g          312 t
ORIGIN      
        1 ccagcaatgg cgaagctgat ccccttcggc ggcaaggcgc cgagcgtcga tccgtcagct
       61 ttcgtcgccc cgggcgcggt tctgatcggc gatatcgaaa tcgggccgga agccagcatc
      121 tggtacaatt gcgtgctgag gggcgacgtc aaccggatcc ggatcggcgc ccgaaccaac
      181 atccaggacg gcagcgtcct ccacgtcgat tcgccgcgtc ccggcagccc cgccggcctt
      241 cccacgatca tcggcgagga cgtcctgatc ggccatatgg cgatggtcca tggctgcctc
      301 ctccacgacc gcgccttcgt cggcctcggc gcgatcgtca tggacggctg cgagatcgag
      361 ggcggtgcca tgctcgccgc cggcgccctg ctcaccccgg gacggcggat cccggccggc
      421 cagctctggg cggggcggcc cgccaaatat gtccgcgatt tgaacgaggc ggaaattacc
      481 ggccagcagg ccggggtcgc ccactacgtc gccctggcca ggctgcactc ggcggcgatc
      541 agaggcggat gaggctttcg ccgaggcgct ggagtcggtc cgcctgggcg tcggtcaggg
      601 cctgcagctc ggcggcggcg cggcgcagcc gttccgaatc ccccgagccg agcgtgcccg
      661 cgctggtccg ctcgaccgcg agccggtcga gatcggccac cgccgcggtc gtccgggccc
      721 gagccgtctc gagcctcgag agggcctgct gggcctcgat ccaggcgtcg cttccagccg
      781 cgcccgcccg gcccgccgcc gcctgcgccg ccggcagcgc cgaatcgaac tcggccgccc
      841 cggcccttcc ggcgtcgagc aggcggtcga tctcggccgc cagcgccgga tcgtcgggct
      901 gcggcgtcgg cttgcgctcc tccgcatcgt cggcataggc cttctcggcc gggcgcggag
      961 cgagcgaagg gtagggcccg ggcgccgcgc agcccgccgc ggcgaggagc ggaaggagga
     1021 tcgcaagggg cggacgaagg ctggccatgc ctcctcctag gcggcgagcc ggagcccggc
     1081 aacaccggcc tccgccggtg caggcggggt tgcgcgccgc cgtccttgcc cctatgtccc
     1141 cctctcccgc gcgcccatag ctcagctgga tagagcacca gactacgaat ctgggggccg
     1201 gaggttcgaa tccttctggg cgcgccattt tcccatcccg gaagggccgc cgcctggcgg
     1261 cagcctcgtc cctccgtgtc cactgtcttc gttcgggtgc tgacggcact tcaccagccg
     1321 agcggtgtct gttctccgaa cgtcaccttg ggtcgaacga aggtttcagc atcgcggcgc
     1381 agctgcttcc agttcggcag gtggaaggga tcgaaagaca gctcgaccag caggcctttc
     1441 tcatattccg tcgccatcgt gcagaccatc ggcacgtggg agacaagctg ctcgcacacg
     1501 atcatggtcg cgccgggagg ttcgatcagc agaaagctgt tgtcgccgaa acgccgatgc
     1561 gtcgtcagac gccgcttcag tccgaagagg tcggtcgccg cctcactgta ggtcgaataa
     1621 tggttgagcc aggtcccgtt cgtcctcttc gctatgctcc cttccctggt ccagccgacc
     1681 cgaaggtagc ccgtcgcgcg atcgccgcgc cggcttgccg gggccggggc gaaatcggcc
     1741 aggtcgacgg tcagtgcggg cagggagctg acgaaatccc gcagatccgg ctggcgtttc
     1801 agatattgga cggggatcgt gatccgtatc cgatcgcggt cgccgtccgg tccgaggtcg
     1861 aaggttcgcg actcgcccgg ttcggcaccc cgggcgggag acccgcaagg cgccagggac
     1921 gccaggacaa gcagcaggag ggcgcgcgga agcatgagac cgctttagcg cagaaaggtt
     1981 agcgaaggcc tttcccgaac t
//
LOCUS       DASWNU010000540         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_358558, whole genome shotgun sequence.
ACCESSION   DASWNU010000540 DASWNU010000000
VERSION     DASWNU010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358558"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VGB57_10680"
     CDS             complement(<1..763)
                     /locus_tag="VGB57_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131857.1"
                     /translation="MHEALRTLVAAELAEPVDPRVSAMAMALAAFYPGAARAVLFYGS
                     CLRQRELDGLMLDFYLIVSDYRSAYGRSWRAPANRLIPPNVFPFEHDGLVAKYAVLSE
                     ADFERLNGPEPLSVSVWARFAQPSRLVWSADEAAATRAVAAVARAAPTLLGEARPLWE
                     GSGDPLDLWRCAFTLTYSAELRAERQGRPASVVEAEPERYRRFSGPALAAAGIDLDAP
                     VPESERARARRRWAARRRAGKALTVLRLVKASGTYA"
     gene            complement(1000..2076)
                     /locus_tag="VGB57_10685"
     CDS             complement(1000..2076)
                     /locus_tag="VGB57_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0131858.1"
                     /translation="MLKRVLGLLAMAWAGAAGAQPDYAGQLKEGDVVLKDFRFGSGDR
                     MAGLRIHYATLGAPRRDSKGAVSNAVMVLHGTGGTGRQFLQPQFANDLFGPGQPLDIS
                     RYYVILPDGIGHGKSSKPSDGMRMRFPQYDYDDMVEAQRRMLVEGLGVTRLRLMMGTS
                     MGCMHIFVWAQAHPDFARALMPTACLPTEIAGHNRMWRKLAVEGIRSDPAWAGGNYAA
                     QPAQGLRTAVSVLQIAGFAPLYLQKAYPTRAAADAYILDRVAKDLPTRDANDLIYQLE
                     ASRTYNPWPKLERIRAPMTWVNSADDFINPADYGIAEDAARRLKTGRYILIRATSDTR
                     GHGTHTWAKFWRQELIDLLARTEE"
     gene            2157..>2477
                     /locus_tag="VGB57_10690"
     CDS             2157..>2477
                     /locus_tag="VGB57_10690"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEY0131859.1"
                     /translation="MEYVGFGLLALGILWVAGFGYAHFRAVGKAKAAETWPTAVGRVI
                     SSEVVEEESTDREGGTSTWYNPVVTYAYSAGGRELTGRRLRFGNYRCASRRKAEAALA
                     PYPAG"
BASE COUNT          389 a          864 c          835 g          389 t
ORIGIN      
        1 cggcataggt gccgctcgcc ttgaccagcc gaagcacggt cagcgccttg cccgcccgcc
       61 gccgcgccgc ccagcgccgc cgggcgcggg cgcgctcgga ctccggcacc ggcgcgtcga
      121 ggtcgatgcc cgccgccgcg agcgccgggc cgctgaagcg ccggtagcgc tccggctccg
      181 cctcgacgac ggacgcgggc cgcccctgcc gctccgccct gagctccgcc gaataggtca
      241 gggtgaaggc gcaccgccac aggtcgagcg gatcgccgct cccctcccac agcggccggg
      301 cctcgccgag cagggtcggc gcggcccgag ccaccgccgc cacggcgcga gtcgcggcgg
      361 cctcgtcggc gctccacacc agccgcgatg gctgggcgaa ccgggcccag accgacaccg
      421 agaggggttc gggcccgttg agccgctcga aatccgcttc gctcagaacc gcatatttgg
      481 cgacaagccc gtcatgctcg aaggggaaga cgttgggcgg gatcagccgg ttggcgggcg
      541 cccgccagct ccgcccgtaa gcggagcgat agtcggagac gatcaggtag aagtcgagca
      601 tgagcccgtc gagctcgcgc tgcctcaggc aagacccgta gaacagcacc gcccgggccg
      661 cccccggata aaacgccgca agcgccatcg ccatcgcgga gactcgaggg tcgaccggct
      721 cggccagctc ggccgcgacg agtgtacgga gtgcttcatg catgccgagg ctatagccgc
      781 cgaacgcggc gaagctaagg ccgcctaaag gttgttcgca caaaggcaca aaggcacaaa
      841 gagtccgcga agcggcttat ccgcaaccgt tcgggaaggc cgtaaggtca gcgccccgtg
      901 aggccccttc gtgcctttgt gcctttgtgc gaacccaata atggccttcg gccagggctc
      961 ggaatgcctc ggatgccctt gggccggttc ggcagccccc tattcttctg tccttgccag
     1021 caggtcgatc agctcctgcc tccagaattt cgcccacgta tgcgtgccgt gaccgcgcgt
     1081 atccgaagtc gcccggatca ggatgtagcg gcccgtcttg agccgccgtg ccgcgtcctc
     1141 ggcgattccg tagtcggccg ggttgatgaa gtcgtcggcg ctgttgaccc atgtcatcgg
     1201 cgcccggatc cgctccagct tcggccaggg attgtaggtg cgcgaggctt cgagctggta
     1261 gatgaggtcg ttggcgtcgc gggtgggaag gtccttcgcc acccggtcaa ggatataggc
     1321 gtcggccgcc gcgcgggtcg gataggcttt ctgcaaatag agcggcgcga agccggcgat
     1381 ctggaggacg ctgacggcgg ttcggagccc ctgcgccggc tgagccgcgt aattgccccc
     1441 cgcccaggcg ggatcgctcc tgatcccctc gacggcgagc ttgcgccaca tccggttgtg
     1501 gccggcgatc tcggtcggaa ggcacgccgt cggcatcagc gcccgggcga agtcgggatg
     1561 ggcctgtgcc cagacgaaga tgtgcatgca gcccatggac gtgcccatca tcagccgcag
     1621 ccgggtaacc ccgagcccct cgaccagcat ccgccgctgc gcctcgacca tgtcgtcata
     1681 atcatattgc gggaagcgca tccgcatccc gtcgctcggc ttcgacgatt tgccgtggcc
     1741 gatgccgtcc ggaaggatga cgtaataacg gcttatgtcg agcggctggc ccgggccgaa
     1801 caagtcgttg gcgaattgcg gctggaggaa ctggcgtccg gttcccccgg tgccgtggag
     1861 caccatgacc gcgttcgaca ccgcgccttt ggaatcccgc cgcggcgcgc cgagcgtcgc
     1921 ataatggatc ctcagccccg ccatccggtc gccgcttccg aagcggaagt ccttgaggac
     1981 gacgtcgccc tccttcagct gcccggcata atccggctga gcgcccgccg cgcccgccca
     2041 ggccatcgcc agcagtccca gaacgcgctt cagcatcgcc gtctcccttg ccttgcgggg
     2101 ctttactccc ggccggcgcc gatgctcaat ggcggcgaca cgagggaggg ttttcaatgg
     2161 aatatgtcgg tttcggcttg ctcgcgctcg gcattctctg ggtcgccggg ttcggctacg
     2221 cccatttccg ggcggtcggc aaagccaaag cggcggagac gtggccgacg gcggtgggcc
     2281 gggtgatctc gagcgaggtg gtcgaggagg aatcgaccga ccgcgagggc ggcaccagca
     2341 cctggtacaa tccggtcgtc acctacgctt attccgccgg cggccgcgag ctgaccggcc
     2401 gccggcttcg cttcggcaat taccgctgcg ccagccgcag gaaagcggaa gcggcgctgg
     2461 ctccctatcc ggccgga
//
LOCUS       DASWNU010000541         5144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_489765, whole genome shotgun sequence.
ACCESSION   DASWNU010000541 DASWNU010000000
VERSION     DASWNU010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489765"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VGB57_10695"
     CDS             complement(<1..567)
                     /locus_tag="VGB57_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0131860.1"
                     /translation="MKFTLSWLKEHLETDADLATVTETLTAVGLEVEGVENAADKLAP
                     FTVARILTAERHPQADKLQVLSVDAGTGEAIQVVCGAPNARAGMLGVFGAPGAYVPGS
                     DLTLKVAAIRGVESRGMMCSVRELELGDEHDGIISLPEDAPVGARYADWAGLNDPVVD
                     VAITPNRQDAMGVHGIARDLAAAGLGNLR"
     gene            complement(668..1738)
                     /gene="pheS"
                     /locus_tag="VGB57_10700"
     CDS             complement(668..1738)
                     /gene="pheS"
                     /locus_tag="VGB57_10700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY0131861.1"
                     /translation="MREFTANTAAAQAASTQINQLDGLKSALLGKKSEFETFLKAVSS
                     ARDVLGHEPVAFANTAKKELFSLYDRKKSALEDGEVEARLATEGLDMTLPAAPAPRGS
                     VHPVSQVMDELAEIFADMGFSVATGPEIEDDWHNFTALNFPPDHPARAMHDTFYLRPQ
                     EGADERMLLRTHTSPVQIRAMMASKPPLYVIAPGRTYRSDSDATHTPMFHQVEGLVID
                     RGIHLGHLKWTLETFLKAYFERDDIVLRLRPSYFPFTEPSAEVDVGFTRVKGRRVLGG
                     TEGWMELLGSGMVHPRVIANCGLDPDEWQGFAFGCGIDRLAMLKYGMDDLRAFFDGDL
                     RWLRHYGFSMLDVPTLSGGVGA"
     gene            2399..3331
                     /locus_tag="VGB57_10705"
     CDS             2399..3331
                     /locus_tag="VGB57_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY0131862.1"
                     /translation="MSWFPISEARDGPTIAALHIYSQIVGKAAVALLPWRNHGWHVTL
                     RAHPRGLRTEPLYGAGGPFELGLDLASHEFTFADGGAVKRLPLRPMTAAIFHGEVMAC
                     LKAAGHEVRIHPATNELDPPIRFDTDDTPRAYDHGSASRLLGALLNADRVFRLFRSSF
                     LGKASPVHFFWGSFDLAVTRFSGRPAPLHPGGIPHLPDAVTREAYSHEVSSAGFWPGG
                     AGGGGEPFFYSYAYPSPEGFGAAPVEPWGARFDETLGEFVLPYEVVRSADDPDSALLR
                     FLESTYRAAADLGRWDRPALECAQGVPKVPRPLA"
     gene            complement(3328..3915)
                     /locus_tag="VGB57_10710"
     CDS             complement(3328..3915)
                     /locus_tag="VGB57_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131863.1"
                     /translation="MKLLAFAAAALLALQAQPGGAQAPQAAPSEALVDRFLAVLPDRE
                     EIQTRSSDIDAGELKALIALNPGKEAKVRAILQANLACSGEAVSAGTLRMLRTVARDL
                     GEARLKRLIAFYEGPDFAAFKALGTRMEGNPTPSAEDKAAMAKLMEAYPLQAYYEGLN
                     RAPDIIATDEGFMSAAVKCASEQMAALEAAGLKSH"
     gene            complement(4179..>5144)
                     /locus_tag="VGB57_10715"
     CDS             complement(4179..>5144)
                     /locus_tag="VGB57_10715"
                     /inference="COORDINATES: protein motif:HMM:NF021382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEY0131864.1"
                     /translation="IQAQRNHFHYVEEAAETLAGALGEPTGIFEALRRRLKEGFGIET
                     RVVPPELVEPTSQAYDFHRKRLMLSAMLRPESRTFGAAYQLALVEFGPMLDRMVDTAG
                     APDVPTRRLLRMSLANYAAGAIMMPYAAFLAAAEKHRYDVDRLCADFGASVEQVSHRL
                     TTLSRTGARGVPFFMLRVDAAGNVSKRFAGESFPFSRFGGTCPRWNLHAAFQTPGRVA
                     IQIVETPDGARFFTIARTVDRSVRLDPRESSQLAIGLGCDVKYAPRILYADGEDLAKP
                     YVTPIGPACTICPRLRCPQRAAAPAGRTLALNETQKTISPYPFVG"
BASE COUNT          820 a         1738 c         1699 g          887 t
ORIGIN      
        1 cctcaggttc ccgagaccgg cggcggcgag gtcgcgggca attccatgca cccccatcgc
       61 gtcctgccgg ttgggcgtga tcgcgacgtc caccaccgga tcgttgagac ccgcccagtc
      121 ggcatagcgc gcgccgaccg gcgcgtcttc cggcagcgag atgatgccgt catgctcgtc
      181 gccgagctcc agctcgcgga ccgagcacat catgccgcgg ctttcgacgc cgcggatcgc
      241 cgccaccttc aaggtgaggt cgctgcccgg cacataggcg ccgggcgccc cgaacacgcc
      301 gagcatgccg gcgcgcgcat tgggcgcccc gcacaccacc tggatcgcct cgccggtccc
      361 ggcatcgacg ctcagcacct gcagcttgtc cgcctgcggg tggcgctcgg cggtcaggat
      421 cctcgcgacc gtgaagggcg cgagcttgtc cgccgcattc tccacgccct cgacctcgag
      481 gccgacggcg gtgagcgttt cggtgacggt ggcgaggtcg gcgtccgtct ccagatgctc
      541 cttcagccag gacaaggtga atttcacagc gcttcctccg tcatcccggc ggaagccggg
      601 acctcgggga gacccgccgg gacttctcgt cctgaggtcc cggctttcgc cgggatgacg
      661 atcgccctca tgcccccact cccccgctca gcgtgggcac gtcgagcatc gagaagccgt
      721 aatgcctcag ccaccgcaga tcgccgtcga agaaggcgcg cagatcgtcc atcccgtatt
      781 tgagcatggc gagccggtcg atgccgcaac cgaaggcgaa gccctgccat tcgtcggggt
      841 cgagcccgca attggcgatc acccgggggt ggaccattcc ggaaccgaga agctccatcc
      901 acccctcggt cccgccgagc acccgccggc ccttcacccg ggtgaagccg acgtcgacct
      961 cggccgatgg ctcggtgaac gggaagtagc tggggcggag gcggaggacg atgtcgtcgc
     1021 gctcgaaata ggccttgagg aaggtctcga gcgtccattt gagatggccc agatgaatcc
     1081 cccggtcgat gacgaggccc tccacctgat ggaacatcgg cgtgtgggtg gcgtcgctgt
     1141 ccgagcggta ggtccggccc ggggcgatga catagagggg cggcttgctc gccatcatcg
     1201 cccggatctg caccggcgag gtatgggtgc gaagcagcat ccgctcgtcg gcgccctcct
     1261 gcggccgcag gtagaaggtg tcgtgcatcg cccgcgccgg atgatccggc gggaaattga
     1321 gcgcggtgaa attgtgccag tcgtcctcga tctccggccc ggtcgcgacc gagaagccca
     1381 tgtcggcgaa gatttcggcg agctcgtcca tcacctggct gaccggatgg acgctgcccc
     1441 gcggcgcggg cgcggcggga agggtcatgt cgagcccctc ggtcgcaagc ctggcctcga
     1501 cctccccgtc ttccagtgcg ctttttttgc gatcgtacag gctgaacagc tcttttttcg
     1561 cggtgtttgc gaaagccaca ggctcatggc cgagcacgtc ccgagcgctg ctcaccgcct
     1621 tcagaaacgt ctcgaactca cttttcttgc ctagtagagc cgacttcagg ccatccagct
     1681 gatttatctg agtggaggcc gcctgcgctg cagcagtgtt cgctgtaaac tcccggatgg
     1741 cattggcgat cgcacttccg ggtggagtag aggcgctttt caagatgctg ttgatcgacc
     1801 tcagagggtc ggccaactgg tccacctgag ccctgttcac cacaggcgaa agcgttattt
     1861 ttttaatcgt ttccaaaacc gaagcgttgg ggtccttcaa aattcctctt aaatcctctg
     1921 caacccgcgc ctcttcaatg cgccggatcg tctccctgat cccgcggatg gagtcgctga
     1981 ttgcattcga tttttcggcc atggttactg cctctgaggc tcatgataga gactgaagga
     2041 atctgtaacc tgcccctaac ctgcggccgc gagcggcggc aagcctctag gcgtcgccgt
     2101 aattggtgcc tggcaccaat cgcgcgctct atgcgggtta ggcggagcgg ttcgtaggca
     2161 gatagggttc gcacgaaggc acgaaggcac gaaggcacga aggacgccgt gcaagatcgg
     2221 cgcgcagcgc ccttcattct cattctggaa aacagtcatt gcggcttcgc cgccgcggca
     2281 cctttgtgcc tttgtgcctt tgtgcgaacc aacacagcct cagccgcggt acagcgctcc
     2341 tgagtccggc acgacagcgg ggcttcccca aaccccgcca agccgacaaa ttgccaccat
     2401 gtcctggttc ccgatctccg aagcccgcga cggtcccacc atcgccgccc tccacatcta
     2461 cagccagata gtcggcaagg ccgccgtggc cctgctgccc tggcgcaacc atggctggca
     2521 cgtcacgctg agggcccatc cgcgcgggct gaggaccgag ccgctctacg gagccggggg
     2581 gccgttcgag ctcgggctgg acctcgcgag ccacgaattc accttcgcgg acggcggcgc
     2641 agtgaagcgc ctgccgctcc ggccgatgac ggcggcgata ttccatggcg aggtgatggc
     2701 ttgcctcaag gccgctgggc acgaggtccg gatccatccc gccaccaacg agctcgaccc
     2761 gcccatccgg ttcgacacgg acgacacccc ccgcgcctat gaccacggca gcgcctcgcg
     2821 cctgctcggc gccctgctga acgccgaccg ggtcttccgc ctcttccgct cgagcttcct
     2881 cggcaaggcg agccccgtcc atttcttctg gggaagcttc gacctcgccg tcacccgctt
     2941 ctccggccgc cccgccccgc tccaccccgg cggaattccc catctccccg acgccgtcac
     3001 ccgcgaagcc tatagccacg aagtctcgag cgccggcttc tggcccggcg gagcgggggg
     3061 cggcggcgag cctttcttct attcctacgc ttatccctcc ccggagggat tcggtgcggc
     3121 gccggtcgag ccgtggggcg cgcggttcga cgaaacgctc ggcgagttcg tcctgcccta
     3181 cgaggtcgtg cgctcggccg acgatcccga ctcggctttg ctccggttcc tggaaagcac
     3241 ctaccgggcc gccgcggatc tcgggcgatg ggaccggccg gcgctggagt gcgcgcaagg
     3301 cgtgccgaaa gtgccgcgcc cgctcgccta gtgcgacttg agccccgccg cctcgagcgc
     3361 ggccatctgc tccgaggcgc acttcaccgc agcgctcatg aagccctcgt cggtggcgat
     3421 gatatcgggc gcgcggttca agccttcata ataagcttgg agcggatagg cctccatcag
     3481 cttcgccatc gcggccttgt cctccgcgct gggggtcgga ttgccttcca ttcgggttcc
     3541 gagcgctttg aaggcggcga agtccggccc ctcgtagaag gcgatcaacc gcttcagcct
     3601 ggcctcgccg aggtcgcggg cgacggtccg gagcattcga agggttccgg cggaaacggc
     3661 ctcgccggag caggcgaggt tggcctgaag gatcgcccgc accttcgctt ccttgcccgg
     3721 attgagggca atcagcgcct tcagctcccc ggcgtcgatg tcgctgcttc gggtctggat
     3781 ctcttcccgg tcgggaagaa cggcgaggaa gcggtcgacc agagcctcgc tcggcgcggc
     3841 ctggggagcc tgggcgccgc ccggttgggc ctggagcgcg agtagcgcgg cggcggcgaa
     3901 ggcgagcagc ttcatgacgt cacttcctga gctggcggct ccccgggggg aggcgctggg
     3961 agcggcgatc ttgttggacc ggcaggccgt tgtccacctg tcgaaggaga agattgtttc
     4021 acacgaagac acgaaggcac aaaggcgtcc gagcggatcg gcgcgcagcg cccttcattc
     4081 cgttgcggcg gagagtcgct gcggcttcgc cgcgacagtg gctttgtgtc tttgtgtgac
     4141 caaaatgcag ccaaagccgc ggcaccgcgc ccgaaggctc accccacgaa cggataaggc
     4201 gagatcgtct tctgggtctc gttgagggcc agcgtccgcc ccgccggagc ggcggcgcgc
     4261 tgggggcagc gcaggcgcgg gcagatggtg caggccgggc cgatcggggt gacgtagggc
     4321 ttggccagat cctcgccgtc ggcatagagg attcttgggg catatttgac gtcgcagccg
     4381 aggccgatgg cgagttgcga gctctcgcgc gggtcgagcc ggacggagcg gtcgacggtc
     4441 cgggcgatgg tgaagaagcg cgccccgtcg ggagtctcga cgatctggat ggcgacccgt
     4501 cccggagtct ggaaggcggc gtggaggttc cagcgcgggc aggtgcctcc gaagcgcgag
     4561 aaagggaagc tctcgccggc gaagcgtttc gagacgttgc ccgccgcgtc gaccctcagc
     4621 atgaagaagg gcacgccccg ggcgccggtt cgggacaggg tggtgagccg gtgcgagacc
     4681 tgctcgacgc ttgccccgaa atcggcgcag agccggtcga cgtcgtagcg atgcttctcc
     4741 gccgcggcga ggaaggcggc ataaggcatc atgatcgccc cagccgcata attggcgagg
     4801 ctcatccgga gcagccgccg ggtcggaacg tccggagcgc cggcggtatc gaccatcctg
     4861 tcgagcatcg gcccgaattc gacgagggcg agctggtagg cggcgccgaa ggtccggctc
     4921 tcgggcctca gcatggccga gagcatcaaa cgcttgcggt ggaaatcgta ggcctggctg
     4981 gtcggctcga ccagttcggg cggaaccacc ctcgtctcga tcccgaaccc ctccttgagc
     5041 cgccgccgga gcgcctcgaa gatcccggtc ggctcgccca gcgccccggc cagggtctcc
     5101 gccgcctcct cgacatagtg gaaatgattg cgctgagcct ggat
//
LOCUS       DASWNU010000542         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_437437, whole genome shotgun sequence.
ACCESSION   DASWNU010000542 DASWNU010000000
VERSION     DASWNU010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437437"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VGB57_10720"
     CDS             <1..678
                     /locus_tag="VGB57_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0131865.1"
                     /translation="HARLRDPATRARVVADMQGGSRDWTTVQPVGFVNPALRVHAGKR
                     LPEIAALRGKSAAETAVDLVAEDGSQVTTIFHSISEDNLRKVLARPWVSFGSDAAAIA
                     TEPPFTDKEVHPRTYGNFARLLGRYVRDEKALSLAEAVRRLTSLPADNLRIADRGRLK
                     RGFHADLALFDPAKIADRATYERPHQYAVGMRHVFVNGVQVLRDGEHTGAKPGRFVRG
                     PGWRKCK"
     gene            675..2357
                     /locus_tag="VGB57_10725"
     CDS             675..2357
                     /locus_tag="VGB57_10725"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY0131866.1"
                     /translation="MRRSIPALALLLAGCATAPSGPSYDSVIRGGTIYDGSGGAPYTG
                     DVGLRGDRIAYVGPRLDGRARKQIDASGLAVAPGFINMLSWANVALIEDPKGQSDIRQ
                     GVTLEVMGEGESMGPLSRQMVLDETQRQGDIRYIVDWRTLGGYLSQMEKKGVSPNIAS
                     FVGATTVRVHELGEADVDPTPAQLDRMRALVREAMNEGAMGLGSSLIYAPAFFAETPE
                     LIALATEAGKCGGMYISHIRDEGPKLLEAIDELVAISAASGTPAEIYHFKQSGRPNWG
                     KIDAAIARVEAARARGLRITADMYTYPASSTGLDAAMPLWVQEGGIEAWVKRLKDPAQ
                     RARVLAEMRSGESEDSALVVKEPSKVLLLGFKNPALKPLTGKTLEEVARERGKTAEET
                     AADLIVEDESRIQVAYFSMSEENVRREVALPWMSFGSDASSQAPEGAFLKSSTHPRAY
                     GNFARVLGKFARDEKVVTLQDAIRRMTSYPAGNLGIKDRGALKPGHFADLAIFDPATI
                     RDNATFARPQQYATGMRHVFVNGVQVLRDGEHTGATPGRFVKGPGAGRCPPA"
BASE COUNT          427 a          885 c          863 g          374 t
ORIGIN      
        1 cacgcgcggc tcagggatcc ggcgacccgg gcccgggtcg tcgccgacat gcaggggggc
       61 tcgcgcgact ggacgacggt gcagccggtc ggcttcgtca atcccgcgct ccgcgtccat
      121 gccggcaagc gcttgccgga gatcgccgcc cttcgcggca agagcgcggc cgagacggcg
      181 gtcgatctcg tggccgagga cggcagtcag gtcaccacca tcttccactc gatctccgag
      241 gacaatctcc gcaaggtgct cgcccggccc tgggtcagct tcggctccga cgcggcggcg
      301 atcgccaccg agccgccctt caccgacaag gaagtccatc cccgcaccta cggcaatttc
      361 gcccggctgc tcggccgcta cgtgcgcgac gagaaggcgc tgagcctggc cgaggcggtg
      421 cgcaggctga cctcgctccc cgccgacaat cttcggatcg ccgaccgcgg ccgcctcaag
      481 cgcggcttcc acgccgatct cgccctgttc gacccggcga agatcgccga ccgcgccacc
      541 tacgagcgcc cccatcaata tgcggtggga atgcgccacg ttttcgtcaa cggtgtgcag
      601 gtgcttcgcg acggcgagca taccggagcg aaaccgggcc gcttcgtgcg cgggcccggc
      661 tggaggaaat gcaaatgaga agatcgatcc cggccctggc cctgctgctc gccggctgcg
      721 ccaccgctcc gtccgggccg agctacgaca gcgtcatccg gggcggaacg atctacgatg
      781 gcagcggcgg cgcgccctat acgggcgatg tcggactccg cggcgaccgg atcgcctatg
      841 tcggcccgcg cctcgacggc agggcgcgca agcagatcga cgcctccggc ctcgccgtcg
      901 cgcccggctt catcaacatg ctgagctggg ccaacgtggc gctgatcgag gacccgaagg
      961 ggcagagcga catccgccag ggggtcaccc tcgaagtgat gggggagggc gaatcgatgg
     1021 ggccgctctc ccgtcagatg gtcctggacg agacccagcg gcagggcgac atccgctaca
     1081 tcgtcgattg gaggaccctg ggcggctacc tctcgcagat ggaaaagaag ggcgtctcgc
     1141 ccaacatcgc cagcttcgtc ggggcgacga cggtgcgcgt gcacgagctc ggcgaggccg
     1201 acgtcgatcc cactccggcg cagctcgacc ggatgcgggc gctggtgcgc gaggcgatga
     1261 acgagggcgc gatggggctc ggctcctcgc ttatctatgc gcccgccttt ttcgccgaga
     1321 ctccggagct gatcgcgctc gctaccgagg cgggcaaatg cggcggcatg tatatcagcc
     1381 acatacggga cgaaggtccc aagctgctcg aagcgataga cgaattggtg gcgatctcgg
     1441 cggcctcggg cacgccggcc gaaatctatc atttcaagca gtccggccgg cccaattggg
     1501 gcaagatcga cgccgccatc gcccgggtgg aggcggcgcg ggcgcgcggc ctcaggatca
     1561 ccgccgacat gtacacctat ccggcgagct cgaccggcct cgacgcggcg atgccgctct
     1621 gggtccagga agggggcata gaagcctggg tgaaacgcct gaaggacccg gcccagcgtg
     1681 cccgggtgct ggccgagatg cgcagcggcg aatcggagga cagcgcgctc gtggtgaagg
     1741 agccgtccaa ggtgctcctc ctcggcttca agaatccggc gctcaagccc ctcaccggca
     1801 agaccctgga ggaagtggcg cgggagcgcg gcaagaccgc cgaggagacc gccgccgacc
     1861 tcatcgtcga ggacgagagc cggatccagg tcgcctattt ctcgatgagc gaggaaaatg
     1921 tccgccgcga ggtcgccctt ccgtggatga gcttcggctc ggacgcttcc tcgcaggcgc
     1981 ccgagggcgc gttcctgaag tcgagcaccc acccccgcgc ctacggcaat ttcgcccgcg
     2041 tcctgggcaa gttcgcccgc gacgagaagg tggtgacgct tcaggacgcg atccggcgga
     2101 tgacgtccta tcccgccggc aatctcggca tcaaggatcg cggcgccctc aagccgggcc
     2161 atttcgccga tctcgccatc ttcgatcccg ccacgatccg cgacaacgcc accttcgccc
     2221 ggccccagca atacgcgacc ggaatgcgcc acgtcttcgt caacggagtc caggtgctga
     2281 gggacggcga gcataccggc gcgactccgg gccggttcgt gaaggggccg ggcgcgggcc
     2341 ggtgccctcc cgcgtagcgg gcctcggccg ctatattttc tcacgcgaag aacgcgaaga
     2401 ggctgcgtca gtccccgccg cctccgcgcg ttcttcgcgt tccttcgcgt gatccaaaat
     2461 ggcgcctgcg gcgcgctgcg accggcaggt tttatacatg gcgggcaacg gaagccgccc
     2521 ggcgcgtttg aacaggcaga atcgggagt
//
LOCUS       DASWNU010000543         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_203452, whole genome shotgun sequence.
ACCESSION   DASWNU010000543 DASWNU010000000
VERSION     DASWNU010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203452"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..884)
                     /gene="accD"
                     /locus_tag="VGB57_10730"
     CDS             complement(30..884)
                     /gene="accD"
                     /locus_tag="VGB57_10730"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY0131867.1"
                     /translation="MSWLNRVRNSIPFLPKRETPENLWHKCRQCNAMIFTKEWEENDS
                     VCPRCDHHGRIGPRARFESLFDPGYSMLPQPQVREDPLKFRDSKRYSDRLRAARTATG
                     ESDALINARGTVDGHRAVIGVQDFAFMGGSMGMAVGAAFLDGARAAIADHCPYVIFTA
                     AGGARMQEGILSLMQMPKTTVAIAELKEAGLPYIVVLTDPTTGGVTASYAMLGDVHIA
                     EPGALIGFAGQRVIESTIREKLPEGFQRAEYLLAHGMVDMVVHRKALRETLARLIGYL
                     TPEARAAA"
     gene            complement(1040..1714)
                     /gene="pyrF"
                     /locus_tag="VGB57_10735"
     CDS             complement(1040..1714)
                     /gene="pyrF"
                     /locus_tag="VGB57_10735"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY0131868.1"
                     /translation="MRSPIFVALDTPDLERAKAIATRVRNHVGGIKLGLEFFCANGRH
                     GVRDMAELHLPIFLDLKLHDIPNTVGKAIQALGPLEPAVLTVHAAGGRAMMEDAKAAA
                     PTGTKVVAVTVLTSLDGSDLEAIGLAGDPRAQVERLTALAGEAGLDGVVCSGNEVAAA
                     RKLWPDGFFVVPGVRPADSRVGDQKRVMTPRKALDAGASILVVGRPITEAEDPDLAAR
                     AIEATL"
     gene            complement(1711..1998)
                     /locus_tag="VGB57_10740"
     CDS             complement(1711..1998)
                     /locus_tag="VGB57_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131869.1"
                     /translation="MVLVLFAKANWNAVTLKLWGGLEADVKLPVLVLAAFLLGFLPTF
                     IVYRARLWSLRRRLEPASVSIAPARPIPSPVAVDNGEGERVATDSKVWPTA"
BASE COUNT          336 a          705 c          761 g          353 t
ORIGIN      
        1 tccccatcgc ttcgcgatgg ggaggcaatt cacgccgccg cccttgcttc gggcgtcagg
       61 tacccgatca gccgggcgag ggtctcgcgc aacgccttcc tgtggacgac catgtccacc
      121 attccgtggg cgagcagata ttcggcgcgc tggaagccct cgggcagctt ctcgcggatc
      181 gtcgattcga tcacccgctg gccggcgaag ccgatcaggg cgccgggctc cgcgatgtga
      241 acgtcgccga gcatcgcgta ggaagcggtg actccgccgg tcgtcggatc ggtgagaacg
      301 acgatgtagg gaagcccggc ctccttcaat tcggcgatcg cgacggtcgt cttgggcatc
      361 tgcatgagcg acaatatgcc ctcctgcatt cgcgctccgc cggccgcggt gaagatgacg
      421 taggggcaat ggtcggcgat cgcggcccgg gcgccgtcga ggaaggccgc gccgaccgcc
      481 attcccatcg atccgcccat gaaggcgaaa tcctggactc cgatcacggc gcgatgcccg
      541 tcgaccgtgc cccgggcgtt gatcagggca tcgctctcgc cggtcgcggt ccgggcggcg
      601 cggagccggt cgctgtagcg cttggaatcc cggaacttga gcggatcctc gcgaacctgg
      661 ggctggggaa gcatcgaata gcccgggtcg aacaggctct cgaaccgggc cctcgggccg
      721 atccggccgt gatggtcgca gcgcgggcag acggaatcgt tctcctccca ttccttggtg
      781 aagatcatcg cgttgcactg ccggcatttg tgccagagat tctccggagt ctcccttttg
      841 ggaaggaagg ggatggagtt gcggacgcgg ttcagccagc tcatcgaagg tctcctgaag
      901 ggcgcgggat aggagggtgg ggggaggctt gtccagcggt ggaggacagg catattcgtc
      961 atcccggcga aagccggggc ctcaggacga gaagtctcgg cgggtctccc cgaggtcccg
     1021 gctttcgccg ggatgacggt cagagagtcg cctcgatcgc cctcgccgcc aggtccggat
     1081 cctccgcctc ggtaatcggg cggccaacga ccaggatcga ggctccggcg tccagcgcct
     1141 ttctcggggt catcacccgt ttctggtcgc cgacacggct atccgccggc cgcacgccgg
     1201 gcaccacgaa gaagccgtcc ggccacaatt tcctggccgc cgccacctcg ttgccggagc
     1261 agacgactcc gtccagcccg gcctcgccgg cgagcgcggt cagccgttcg acctgggcgc
     1321 ggggatcgcc ggcgaggccg atcgcctcga ggtcgcttcc gtcgaggctg gtcaggaccg
     1381 tcacggcgac caccttggtt cccgtcgggg cggccgcctt ggcgtcctcc atcatcgccc
     1441 gcccgccggc ggcgtggacg gtgagcaccg ccggctccag cgggccaagc gcctggatcg
     1501 ccttgccgac cgtgttggga atgtcgtgga gcttcaaatc gaggaagatg ggaagatgga
     1561 gctcggccat gtcgcggact ccgtggcggc cgttggcgca gaagaattcg aggccgagct
     1621 tgatcccgcc gacatggttc ctgacccggg tggcgatcgc tttggcccgt tcgagatcgg
     1681 gcgtgtcgag ggcgacgaag atcgggctcc tcatgcggtc ggccagacct tgctgtcggt
     1741 ggcgacccgc tcgccctcgc cattgtcgac ggcgaccggc gacgggatcg ggcgcgccgg
     1801 ggcgatgctg accgaggcgg gctcgagccg ccgcctgagg ctccacagcc gcgcccggta
     1861 gacgatgaag gtcggaagga agccgagcag gaaggcggcg aggacgagga ccggcaattt
     1921 cacgtccgcc tcgagcccgc cccacaactt cagggtaacg gcgttccagt tcgccttggc
     1981 gaacaggacg aggaccacgg tcagggcgat ccagaagagg gttttcagga actgcagtcg
     2041 cgccctccgg ggttcggtac aggtctatgg cggcgcgcgc ggcggcgcaa tggccacgcg
     2101 tcgccccagc gaaggctggg ccccgctggg tgaagaacgc ggtgcttttc actcg
//
LOCUS       DASWNU010000544         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_280818, whole genome shotgun sequence.
ACCESSION   DASWNU010000544 DASWNU010000000
VERSION     DASWNU010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280818"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGB57_10745"
     CDS             <1..377
                     /locus_tag="VGB57_10745"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEY0131870.1"
                     /translation="TAMLPESARAVMGVPHPSGRAAMKMLEREGFNFDCYIDIFDGGP
                     TMTAPTDQIRTVRESRELVLAEVADEVEGPNMMLAHGRLAGFAACCGDVRIAEDETAT
                     LGRETAALLGIEPGDTFLAMGR"
     gene            378..>1541
                     /locus_tag="VGB57_10750"
     CDS             378..>1541
                     /locus_tag="VGB57_10750"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEY0131871.1"
                     /translation="MPLAEINFDGIIGPSHNYSGLSLGNRASATNKGLVSHPRAAALQ
                     GLEKMRHNLRLGLPQGLLLPHRRPDRAWLAELGTRVEDVPASLRPAAFSASAMWAANA
                     ATVSPAPDTADGRCHLTVANLRTMAHRSHEWPETLAQLRLAFADEARFAVHPPVPATF
                     GDEGAANHMRLSASHGAPGVEVFVYGIRGGPFPVRQHDEASRAIARLHGLDPDRTLFV
                     AQSEEAIAAGAFHNDVVAVANGHVLFAHEQAFEARPSFYAELKRLLPELEIVEVPASA
                     ISLEDAIRSYLFNAQLVTLPAGGMALILPEEARETPSVWTWLQAMVAGNGPIRGLFVV
                     DVRQSMANGGGPACLRLRVVADPETVDPRFLVDEDRLDRLAAIVDAEWPDRIEP"
BASE COUNT          231 a          594 c          494 g          222 t
ORIGIN      
        1 acaccgccat gctgcccgag agcgccaggg cggtgatggg agtcccgcac ccctccggcc
       61 gggcggcgat gaagatgctc gagcgcgaag gcttcaactt cgactgctac atcgacattt
      121 tcgacggcgg accgaccatg accgcgccga ccgaccagat ccgcaccgtc cgcgaatcgc
      181 gcgagctcgt gctggccgaa gtggccgacg aggtcgaggg tccgaacatg atgctggccc
      241 acggccgcct cgccggtttc gcggcctgtt gcggcgacgt ccggatcgcg gaggacgaga
      301 ccgcgaccct cggccgcgaa accgccgcct tgctcggcat agagccgggc gacaccttcc
      361 tcgccatggg ccgatagatg ccgctcgccg agatcaattt cgacggaatc atcggcccga
      421 gccacaatta ttcgggcctg agcctcggca atagggcctc ggcgaccaac aagggcctgg
      481 tctcgcaccc gcgcgccgcc gcgcttcagg ggctggagaa gatgcggcac aacctccgcc
      541 tcggcctgcc ccagggcctt ctcctccccc accgccggcc cgaccgggcc tggctggccg
      601 agctcggcac ccgggtcgag gacgtgccgg cctcgctccg cccggctgct ttctccgcct
      661 cggcgatgtg ggccgccaac gccgccaccg tctctccggc cccggacacg gccgacggcc
      721 gctgccacct caccgtcgcc aatctgcgca ccatggccca tcgcagccac gaatggcccg
      781 agaccctcgc ccagctccgt ctcgccttcg ccgacgaggc gcgattcgcg gtccaccccc
      841 cggtccccgc cacgttcggc gacgaaggcg cggccaacca tatgcgcctg agcgcctcgc
      901 acggcgcgcc gggggtggag gtgttcgtct atggaattcg cggcggcccc ttcccggtcc
      961 gccagcatga cgaggcgagc cgagcgatcg cgcgcctcca cggcctcgac ccggatcgca
     1021 ccctcttcgt cgcccagtcc gaggaagcga tcgccgccgg cgccttccac aacgacgtcg
     1081 tcgccgtcgc caacggacat gtcctcttcg cccacgagca ggccttcgag gcgaggccga
     1141 gcttctacgc cgagctgaag cggctgctgc ccgaactcga gatcgtcgag gttccggcga
     1201 gcgcgatcag tctcgaggac gcgatccgga gctacctctt caacgcccaa ttggtcaccc
     1261 tgcccgccgg cggaatggcc ctcatcctgc ccgaggaagc ccgcgagacc ccgagcgtct
     1321 ggacctggct tcaggcgatg gtcgccggca acggcccgat ccgcgggctg ttcgtcgtcg
     1381 acgtccgcca gtccatggcc aatggcggcg gccccgcctg cctgaggctc agggtcgttg
     1441 cggatccgga gacggtggac ccgcggttcc tggtcgacga ggaccggctc gaccgcctcg
     1501 cggcgatcgt cgatgccgaa tggcccgacc ggatcgagcc g
//
LOCUS       DASWNU010000545         4952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_331679, whole genome shotgun sequence.
ACCESSION   DASWNU010000545 DASWNU010000000
VERSION     DASWNU010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331679"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="VGB57_10755"
     CDS             complement(<1..1754)
                     /locus_tag="VGB57_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY0131872.1"
                     /translation="MILVAYDVAAMMFALVAAFSARLGELYLPVDPVVLLAAGISIVV
                     GLIGLFQLRIYHIVLRFFDLGTVSRILFGAAIAATAWVILVYFMRASMVVDGIRILVP
                     RSVGFIYGGFLFVLLFMGRYAMAALVIGAERKHPTGRDGRRNIVIYGANSAGISLAES
                     VRRDRHFRLCGFVDDDPALKGQIIAGAQIFPPRALAGLVEDNAISEVFLAMPKATRSQ
                     RLSAIERLNKLNVRVMTVPAPEEIVSGRFTVSDVRPIDVNDLLRRDPVEPLSDLIKEV
                     VDGQTILVTGAGGSIGSEICRQIIRVRPAKLVLLDHSEFALYTIEQQLIELLRERDPG
                     DRPAIVPVVGSMLNELLVRDIIRMHGVETIYHAAAYKHVPLLEHNEVVGVENNVIGTW
                     VLARAAFDAKLTRFTMISTDKAVRPESVMGASKRVAELVIQALAEQGETRFGIVRFGN
                     VLDSSGSVVQTFREQIRRGGPVTVTHPEVTRFFMSIPEATQLVLQASAMARHGEVFVL
                     DMGEPIRIAELARNMISLSGMTVQDDDNPAGDVEIAYVGLRPGEKLYEELFVGENALD
                     TAHPRIKMAKERFISFAEL"
     gene            complement(1851..3053)
                     /locus_tag="VGB57_10760"
     CDS             complement(1851..3053)
                     /locus_tag="VGB57_10760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0131873.1"
                     /translation="MNAKPRAEAPAPAEDLRQGPEEHWPYYAEDEIEAVARVLRSGRV
                     NQWTGAEVFAFQDALARTFGGKHCIALANGSLALELPLRAFGIGPGDEVVVAPRTFVA
                     SATCAMLTGATPVFADIDPDSGNITAETIEAVLTERTRAIIPVHIGGWPCDMPAIMDL
                     AERRGLLVIEDCAQSHGAEIDGRPAGSFGQAAALSFCQDKIISTGGEGGAVLFRDEAA
                     WDWAWSFKDHGKNRRKVETPPAGPGFRWLHDGVGTNWRLTGPQAAIGLRQLEKLPEWH
                     SARTRNAGIWAEALTDLPGIRVPLPGPRFRHAYYRFYFYLEPGAEDARQEILARAGEA
                     GLRLFTGSCSEVYLEEAFRDLPRPDLPNARAMTRTGLAVEIHPTLRPERLRLRAEKLA
                     ELIRAVVG"
     gene            complement(3095..4294)
                     /locus_tag="VGB57_10765"
     CDS             complement(3095..4294)
                     /locus_tag="VGB57_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131874.1"
                     /translation="MTPVGLIGILISLAGFHFARAGESRARLVLFVLLLLTHIGASLA
                     YYLYAQEFGGDVLLYYYDQWGIYHEGHSGLSTVFVINFVQFLKEYLGGAFFDYFLIFQ
                     AMGFWGILFVLRTFDDIHGELGQPLFSRIYLLLFLPGLHFWTSAIGKDAPVFLGVAMC
                     TWAAFRLQKRYLVFGAGLAIALVIRPHITLIALVALAFTVLVGRNTRLLTRVALLGVT
                     VVGVVSVAGLVSSSFNGLNLSNTDEVSEFLETKSSVSEDSGGDVSITGAAFPVKLLSL
                     LFRPFFIDATGMLGYIASLENVVLLIIILTLFRRFGTSLQVARGAMFARFAFIFFAML
                     TLLLAMVNYNVGLGLRQKMMMMPCLLAFFVALLAVRAARRQAAVYDPRAAAYAGGPLG
                     VPGYRSV"
     gene            complement(4291..>4952)
                     /locus_tag="VGB57_10770"
     CDS             complement(4291..>4952)
                     /locus_tag="VGB57_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131875.1"
                     /translation="DWFAPGRLSYLLKVAAAADADAVSDDQLLIREGADKPWTSIYEL
                     AGWKQSEEGRLLSAAEMCHRDWILKAMFRTAWLRRHGLRFHTVRNWAGEDFEFYMNAI
                     LSGARWVTAREARYFYRSRPGQLTGNRAIAEGIIESLEAMARDPRISGDPELARAMSD
                     RIGRVRSAQWVGFFADAVRGRRWLRAAAMVLRSPARVADAAVALRRRLQFQRRWKEAG
                     A"
BASE COUNT          892 a         1612 c         1719 g          729 t
ORIGIN      
        1 agctcggcga aggagatgaa gcgctccttg gccatcttga tccgcgggtg ggcggtgtcg
       61 agggcgttct cgccgacgaa cagctcctcg tacaatttct cgcccggacg aaggccgaca
      121 taggcgattt cgacgtcgcc ggccgggttg tcgtcgtcct ggaccgtcat tccggacagc
      181 gagatcatgt tgcgcgcaag ctccgcgatc cggatcggct cgcccatgtc gagcacgaag
      241 acctcgccgt ggcgggccat ggcgctcgcc tggagcacga gctgggtcgc ctccgggatc
      301 gacatgaaga agcgggtgac ctcgggatgg gtgacggtga cggggccgcc gcggcggatc
      361 tgctcccgga aggtctgcac caccgagccc gagctatcga gcacgttgcc gaagcggacg
      421 atcccgaagc gggtctcgcc ctgctcggca agcgcctgga tgaccagctc ggcgacccgc
      481 ttgctcgcgc ccatcacgct ttcggggcgg accgccttgt cggtcgagat catggtgaag
      541 cgggtgagct tggcgtcgaa ggcggcgcgg gcgagcaccc aggtgccgat gacgttgttc
      601 tcgacgccca ccacttcgtt atgctcgagc agcggaacgt gcttgtaggc ggcggcgtgg
      661 tagatggtct cgacgccgtg catccggatg atgtcgcgga cgagcagctc gttgagcatc
      721 gagccgacca ccggaacgat cgccgggcga tcgcccggat cgcgttccct gagcagctcg
      781 atgagctgct gctcgatcgt gtagagcgcg aactcgctgt ggtcgagcag gaccagcttg
      841 gccggccgga cccggatgat ctggcggcag atctccgagc cgatcgagcc gccggcgccg
      901 gtgacgagga tggtctggcc gtccaccacc tccttgatga ggtcgctcaa tggctcgacc
      961 gggtcccggc gcaagaggtc gttgacgtcg atcggtcgga cgtcgctcac ggtgaagcgg
     1021 ccggagacga tctcctccgg cgccggtacg gtcatcaccc ggacgttgag cttgttgagc
     1081 cgctcgatcg cgctcagccg ctgcgatcgg gtcgccttgg gcatggcgag gaacacctcg
     1141 cttatcgcat tgtcctcgac gaggccggcg agggccctgg gcgggaagat ctgggccccg
     1201 gcgatgatct ggcccttgag cgcgggatcg tcgtcgacga agccgcagag ccggaaatgg
     1261 cgatcgcggc ggaccgactc ggcgaggctg atgccggcgg aattggcgcc gtagatgacg
     1321 atgttgcggc gcccgtcgcg cccggtggga tgcttgcgct cggcgccgat caccagggcg
     1381 gccatcgcgt agcggcccat gaacagaagc acgaagagga agccgccgta gatgaagccg
     1441 accgatcggg gcaccaggat ccggatcccg tcgaccacca tcgaagcgcg catgaagtag
     1501 acgaggatca cccaagcggt cgcggcgatg gccgcgccga agaggatccg gctgaccgtg
     1561 ccgagatcga agaaccggag cacgatatgg tagatgcgga gctggaagag gccgatcagc
     1621 ccgacgacga tcgagatgcc cgcggcaagc aggaccaccg gatccaccgg cagatagagc
     1681 tcgccgagac gcgccgagaa ggccgcgacc agggcgaaca tcatcgcggc gacgtcgtac
     1741 gccaccagga tcatgcgctt ctgcgcgcgg tccacagagg acactcgtct gacaaagtgg
     1801 aagatcatgg gagtggcggt ccttagacca aattggccgg cgctggaagc ttaaccgacc
     1861 accgcgcgga tcagctcggc gagcttctcg gcccgaagcc ggagccgctc gggccggagg
     1921 gtggggtgga tttcgacggc aaggccggtt cgagtcatcg cccgggcgtt cggcagatcg
     1981 ggccgcggga ggtcgcggaa cgcctcctcc agatagactt cggagcagga gccggtgaac
     2041 agccggagcc cggcctcgcc ggcgcgggcc agtatctcct ggcgcgcgtc ctcggcgccc
     2101 ggctcaagat agaaatagaa acggtaataa gcgtggcgga agcgcggccc cggcagcggc
     2161 acccgaattc ccggcaggtc cgtcagcgcc tcggcccaga ttccggcatt gcgggtgcgg
     2221 gcggaatgcc actcggggag cttttccagc tgcctgaggc cgatcgccgc ctgcggcccg
     2281 gtcagccgcc agttggtgcc gacgccgtcg tgaagccagc ggaagcccgg cccggcgggc
     2341 ggcgtctcga ccttgcggcg gttcttgcca tggtccttga agctccacgc ccagtcccag
     2401 gcggcctcgt ccctgaacag gaccgccccg ccctcgccgc cggtgctgat gatcttgtcc
     2461 tgacagaagg agagcgcggc cgcctggccg aagcttccgg cggggcggcc gtcgatctcg
     2521 gcgccgtgcg actgggcgca atcctcgatg acgagcagac cgcggcgctc ggccaggtcc
     2581 atgatcgccg gcatgtcgca gggccagccg ccgatgtgaa cggggatgat cgcacgggtc
     2641 cgctcggtca gcaccgcctc gatcgtctcg gcggtgatgt tgccgctgtc gggatcgata
     2701 tcggcgaaga ccggcgttgc gccggtcagc atggcgcagg tcgccgaggc gacgaaggtg
     2761 cgaggcgcca ccaccacctc gtcgcccggg ccgatcccga aggcgcggag cggcagctcg
     2821 agggcgagcg agccgttggc gagcgcgatg caatgcttgc cgccgaacgt tcgcgcgagc
     2881 gcgtcctgga aagcgaagac ctcggcgccg gtccactggt tgacccggcc cgacctcagg
     2941 acccgcgcca ccgcctcgat ctcatcctcg gcataataag gccaatgctc ctccggtccc
     3001 tgccgcaggt cttccgccgg agcgggcgct tcagccctcg gtttcgcgtt caacatgctc
     3061 ttccaaaccc tttcggcccg ccgggcggcg ccgttcagac gctcctgtag cccggcaccc
     3121 ccaacgggcc gcccgcataa gctgccgcgc gcgggtcgta aaccgccgcc tggcggcggg
     3181 ccgcgcgcac cgccagtagc gcgacgaaga aggcgagcag gcagggcatc atcatcatct
     3241 tctgcctcag cccgaggccg acattgtagt tcaccatcgc gagcaggagg gtcagcatgg
     3301 cgaagaagat gaaggcgaag cgggcgaaca tggctccccg ggccacctgc aaggacgttc
     3361 cgaaccgcct gaacagagtc aggatgatga tcagcagcac gacgttctcg agcgaggcga
     3421 tgtagccgag catccccgtg gcgtcgatga agaagggccg gaacagcagg ctgagcagct
     3481 tcaccgggaa ggccgcgccg gtgatgctga cgtcgccgcc cgaatcttcg gagacgctgg
     3541 acttcgtctc gaggaattcg ctcacctcgt cggtgttcga caggttgagg ccgttgaagc
     3601 tgctcgagac gagccctgcg acggagacga ccccgaccac cgtcacgccg agcagcgcga
     3661 ctcgagtgag gagtcgggtg ttgcggccga ccagaaccgt aaaggcgagg gccaccaggg
     3721 cgatcagggt gatgtgcggc cggatcacca gggcgatcgc aagccccgcg ccgaagacca
     3781 gatagcgttt ctgcaggcgg aaggcggccc aggtgcacat cgcgacgccg agaaagaccg
     3841 gagcgtcctt gccgatcgcg ctcgtccaga aatggaggcc gggcaggaac agaagcaggt
     3901 agatgcggct gaacagcggc tggcccagct ccccgtggat gtcgtcgaac gtcctcagca
     3961 cgaacaggat gccccagaac cccatggcct ggaaaatcag gaagtaatcg aagaaggcgc
     4021 cgccgagata ctccttcagg aattgcacga aattgatgac gaagacggtg ctgaggccgc
     4081 tgtggccctc gtggtagatg ccccactggt cgtaataata gagaagcacg tcgccgccga
     4141 attcctgcgc atagagataa taagcgagcg aggcgccgat atgcgtcagc agcagcagca
     4201 cgaacaggac caggcgcgcg cgcgactccc cggcccgggc gaaatggaag ccggcgagcg
     4261 agatcaggat gccgatgagg ccgaccgggg tcatgcgcct gcctccttcc atcgcctttg
     4321 gaactgcagc cgccggcgca gcgccacggc ggcgtcggcg acgcgcgcgg gggagcgcag
     4381 caccatcgcg gcggcccgca gccaccggcg cccccgcacc gcgtcggcga agaaaccgac
     4441 ccattgcgcc gaccttacgc gcccgatccg gtctgacatg gcccgggcga gctccgggtc
     4501 gccgctgatc cgggggtccc gggccatcgc ctcgagggac tcgatgatcc cttccgcgat
     4561 agcccggttg ccggtcagct gaccgggccg ggagcggtag aagtagcggg cttcgcgtgc
     4621 cgtcacccag cgcgcgcccg agagaatggc attcatatag aactcgaaat cctcgccggc
     4681 ccagttgcgg accgtatgaa aacgcaggcc gtgccggcgc aaccaggcgg tgcggaacat
     4741 cgccttgagt atccagtcgc gatggcacat ttcggccgcc gagagaagcc gtccctcttc
     4801 ggactgcttc cagccggcga gctcgtagat ggaggtccag ggcttgtcag ccccttcccg
     4861 gatgagcagc tgatcgtcgc tgaccgcgtc cgcgtcggcg gcggcggcga ccttcagcag
     4921 ataggacagc cggcccggag cgaaccagtc gt
//
LOCUS       DASWNU010000546         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_383911, whole genome shotgun sequence.
ACCESSION   DASWNU010000546 DASWNU010000000
VERSION     DASWNU010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383911"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGB57_10775"
     CDS             <1..445
                     /locus_tag="VGB57_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0131876.1"
                     /translation="LLSGSGKNKDGNRVQEISEISRGLKTLAKDLNVPVLALSQLSRA
                     VESREDKTPMLSDLRESGSIEQDADMVWFVYREEYYLANKQPADDHSDFPAWQEEMNR
                     AYGTAELIVAKQRHGSTGKVKMKFEAKITKFSDHIDEGYLPEARG"
     gene            703..>2102
                     /locus_tag="VGB57_10780"
     CDS             703..>2102
                     /locus_tag="VGB57_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0131877.1"
                     /translation="MTLIFKTMAAAILMTTVAGTALAQAAADPHEWLEDVRGEKALGW
                     AKAESEKTLKGFQSDRRYQGLHDRALEVLQAKDRIPMVQMRPDGLYNFWQDQTNVRGL
                     LRRTTLASYRTAEPVWEPVLDIDALAKADGKSWVYKGMQCLPPEMTRCLVNLSEGGGD
                     ATVIREFDTATRRFVDGGFSLAEGKQDADWEDKDTIIVARDWGPGTMTESGYAFVVKR
                     LRRGQGLDKAIEIYRGEPSDVGAGAFVLRDSAGRSHGLIGYRSVGAREMRFTLLRPNG
                     NVPLNLPARANLSGIVDGRLLVQLSEPWAAPGGARFATDSLISYDLEQWKRDPLTARP
                     DLVFAPGARQTLSGIATTKGRLLVNLLDNVRGRAFVYDYSGGKWRSTEIALPGNSTIN
                     IATASDTEDQAMFAVADFLTPSTLWFYDAATGRLETVKTTPPRFDASRHVVEQLEATS
                     RDGTKIPYFLIRPKTM"
BASE COUNT          390 a          696 c          683 g          333 t
ORIGIN      
        1 gctgctcagc ggctcgggca agaacaagga cggcaatcgc gtccaggaaa tctcggagat
       61 cagccgcgga ctgaagacgc tggcaaagga tctcaacgtg ccggtgttgg cgctctccca
      121 gctgtcgcgt gcggtcgaaa gccgcgagga caagacgccg atgctgtcgg atctgcgcga
      181 atcggggtcg atcgagcagg acgcggacat ggtctggttc gtctatcgcg aggaatatta
      241 tctggcgaac aagcagcccg ccgacgacca ttcggatttc cccgcctggc aggaggagat
      301 gaaccgcgcc tacggaaccg ccgagctgat cgtcgccaag cagcgccacg gctcgaccgg
      361 caaggtgaag atgaagttcg aggcgaagat cacgaaattc tccgaccata tcgacgaggg
      421 ctacctgccc gaggcgcggg gatagccccc cgtcatcccg gcgaaagccg ggacctcggg
      481 gagacccgcc gagcctttct tccacgaggt cccggctttc gccggaacga cgggccggga
      541 atgcctggga tcgagcaaga tcggcccatt gttcaagctc atggcggcgg ctaggactcc
      601 ctcacccgtc attgcgagga gcgtagcgac gcggcaatcc agcgggcctc ggaggctctg
      661 gattgcttcg cttcgctcgc aatgacggcg taggagactc gaatgacctt gattttcaaa
      721 acgatggcgg cggcgatttt gatgacgact gttgcaggaa cggcattggc gcaggcggcg
      781 gctgatccgc acgaatggct cgaggatgtg cgcggcgaaa aggcgctggg ctgggcgaag
      841 gccgagagcg aaaagacgct caagggcttc cagtcggacc ggcgctatca gggcctccac
      901 gaccgggcgc tcgaggtgct tcaggcgaag gaccggattc cgatggtcca gatgcggccc
      961 gacggcctct acaatttctg gcaggaccag accaacgtcc gcggcctgct tcggcggacc
     1021 accctcgcca gctatcggac cgcggagccc gtctgggagc cggtgctcga catcgacgcg
     1081 ctcgccaagg cggacggcaa gtcctgggtc tataagggga tgcagtgcct gcccccggaa
     1141 atgacccgct gcctcgtcaa tctctccgag ggcggcggcg atgcgaccgt catccgcgaa
     1201 ttcgacaccg ccacgcgccg cttcgtcgac ggcggcttct cgctcgccga aggtaagcag
     1261 gacgccgact gggaagacaa ggacacgatc atcgtcgccc gcgactgggg gccggggacg
     1321 atgacggaat cgggctatgc cttcgtcgtc aagcgccttc gccgcggcca gggcctcgac
     1381 aaggccatcg aaatctatcg cggcgagccg agcgacgtgg gcgccggcgc gttcgtgctt
     1441 cgggacagcg ccggccggtc gcacgggctg atcggctatc gaagcgtcgg cgcccgcgag
     1501 atgcgcttca ccctgctccg gccgaacggc aatgtccctc tcaacctgcc cgctcgggcg
     1561 aatctctccg gcatcgtcga cggcaggctc ctcgttcagc tcagcgagcc ctgggcggca
     1621 cccggcggcg cccgcttcgc gaccgactcg ctcatctcct acgatctcga gcaatggaag
     1681 cgggacccgc tcaccgcccg gcccgacctc gtcttcgcgc ccggtgcgcg ccagacgctg
     1741 agcggcatcg cgacgaccaa gggccggctg ctcgtcaacc ttctcgacaa tgtccgcggc
     1801 cgagccttcg tctacgatta ttccggcggc aaatggcggt cgaccgagat cgcgcttccg
     1861 ggcaattcca cgatcaacat cgcgaccgcg tccgataccg aggaccaggc gatgttcgcg
     1921 gttgccgact ttctcactcc gtccaccttg tggttctacg acgccgcgac gggcaggctg
     1981 gagaccgtca agacgacccc gcccaggttc gacgcctccc gccacgtcgt cgagcagctc
     2041 gaggcgacct cgcgcgacgg gacgaagatc ccatatttcc tgatccggcc caagacgatg
     2101 aa
//
LOCUS       DASWNU010000547         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_461745, whole genome shotgun sequence.
ACCESSION   DASWNU010000547 DASWNU010000000
VERSION     DASWNU010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461745"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VGB57_10785"
     CDS             complement(<1..650)
                     /locus_tag="VGB57_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY0131878.1"
                     /translation="MTPLDEEQQRARTWFESLRDRICAEFEAIEREAGSAAAFDYTPW
                     QRHDPDGSPGGGGVRGLMKGEVFEKVGVNVSTVAGRFSEDFAATIHGAGEDPRFFATG
                     ISLVAHMANPHAPAVHMNTRFLVTTKRWFGGGADLNPPIPYDEDTADFHARLRAACAA
                     HDQTFYDRFSKWADDYFYIPHRGVHRGVGGIFYDHLEGPFEGTFAYTRGVGEAFLD"
     gene            848..1516
                     /locus_tag="VGB57_10790"
     CDS             848..1516
                     /locus_tag="VGB57_10790"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0131879.1"
                     /translation="MTDNATSLPEGAGATTGGNGGNASTEWRASPGSTHREIEVFETR
                     PSAAVVAGAALAGAIAGAAIPFMFSGRGWSATRQETATVEESVIINRSPREIYDFWRD
                     FTNLPQFMDNIESVTRINEVRSHWVIKAPAGTSVEFNSLVTEDIPGRLIAWKSEEGAT
                     VPNRGRVEFIETSDGTGTNVRTTISYEPPAGAFGRVVAKLFQREPGVQARQDLDRLKQ
                     LLER"
     gene            complement(1555..1689)
                     /locus_tag="VGB57_10795"
     CDS             complement(1555..1689)
                     /locus_tag="VGB57_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131880.1"
                     /translation="MAKWSFVQNEDVPAPPAMPNVFSVHFRAAEQVEDWSLTQVQGDA
                     "
     gene            complement(1972..2256)
                     /locus_tag="VGB57_10800"
     CDS             complement(1972..2256)
                     /locus_tag="VGB57_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEY0131881.1"
                     /translation="MSDEIGDLHQRMEEAAAAMDFEEAKRLRDIIALMRGGASRKEAE
                     KADSFGLTRQQPGRMGLGTSQQRMTPPEGWTPPPKPDPMTSGRSRRKGRP"
BASE COUNT          370 a          756 c          780 g          364 t
ORIGIN      
        1 atgtccagaa aggcctcgcc gacgccgcgg gtgtacgcga acgtgccctc gaacggcccc
       61 tccagatgat cgtagaagat cccgccgacg ccgcgatgga cgccgcgatg gggaatgtag
      121 aaataatcgt ccgcccattt cgagaagcga tcgtagaagg tctgatcatg ggcggcgcag
      181 gcggcgcgca gtcgggcgtg gaagtcggcc gtgtcctcgt cgtaggggat cggcgggttg
      241 agatcggcgc cgccgccgaa ccagcgcttc gtggtgacga ggaagcgggt gttcatatgg
      301 acggccggcg cgtgcggatt ggccatgtgg gcgacgaggc tgatgccggt ggcgaagaag
      361 cgcgggtcct cgccggcgcc gtggatggtg gcggcgaaat cctcgctgaa gcgccccgcg
      421 acggtggaga cgttgacccc gaccttctcg aacacctcgc ccttcatgag gccgcggacc
      481 ccgccgccgc cgggcgagcc gtcggggtcg tggcgctgcc agggcgtata gtcgaaggcc
      541 gccgcgctcc cggcctcgcg ctcgatcgcc tcgaactcgg cgcagatccg gtcgcgcagg
      601 gactcgaacc aggttcgggc gcgttgctgt tcttcgtcga gcggggtcat cggccccgga
      661 tgccgcccgg cgcggcgact tgcaagcgac ctcggcggtc ccccctccat gcggcgggtg
      721 gcggaaaccg tccggcgcaa ttcgccaata gtcggggaac gccgggaatc ctgcgcggtt
      781 tggcggatcg aaggcggccg tgcttcggcc gccccccaat cgattcggcc agtatggagt
      841 gtcaacgatg actgacaatg cgacttccct tcccgagggc gcgggtgcga cgacgggcgg
      901 caacggcggc aacgcttcga ccgagtggcg ggcgagcccc ggctccaccc accgggaaat
      961 cgaggtcttc gagacccgcc cgagcgcggc cgtcgtggcc ggggccgctc tcgccggagc
     1021 gatcgccgga gcggcgatcc ccttcatgtt ctcgggccga ggctggagcg caactcgcca
     1081 ggagaccgcc accgtcgagg aatcggtgat catcaaccgc tcgccgcggg agatctacga
     1141 tttctggcgg gacttcacga acctcccgca gttcatggac aacatcgaat cggtgaccag
     1201 gatcaacgag gtccgatcgc actgggtgat caaggctccg gccgggacca gcgtcgagtt
     1261 caacagcctc gtcaccgagg acatccccgg aaggctgatc gcctggaagt ccgaggaagg
     1321 ggccactgtg cccaaccgcg gccgggtcga gttcatcgag acttcggacg gaaccggcac
     1381 caacgtgaga accaccatct cctacgagcc gccggctggc gctttcggcc gcgtcgtcgc
     1441 caagctgttc cagagggagc cgggggtcca ggcccgccag gacctcgacc ggttgaagca
     1501 gcttctcgag cgataggagg gagcggggcg tctgcgagga cgcccccgct tgcctcaagc
     1561 gtcaccctga acttgtgtca gggaccagtc ctccacctgc tcggcggctc gaaagtggac
     1621 gctgaacacg ttgggcatgg cgggcggagc cggaacgtcc tcattctgga cgaaactcca
     1681 ctttgccatt tgttccttct atgttctaat ccgtcgatgc gcgggaagag gaacagcagg
     1741 catcggcaaa tcgacgccgc cgcgaaggcg gctttcctgg ccggacttcg cgaaggcccg
     1801 cgccgcgagg acgccgccgc cgccgccggg tttagcctga tgggcttcta caacgcacgc
     1861 gcccgagacc cgcgacccct ctccctggag gtgaggggga aaggttcgca aagcgaatca
     1921 gggtgagggg gcgcgtcgta gtggaacggc gccggccgcc ttttttggaa cctagggcct
     1981 gcccttgcgg cgcgagcgtc cgctggtcat cgggtccggc ttcgggggag gggtccagcc
     2041 ttccggagga gtcatccgct gctggctggt gccgagcccc atccgccccg gctgctgccg
     2101 ggtaagcccg aaagaatccg ctttctccgc ttccttccgg ctcgcgccgc cgcgcatgag
     2161 cgcgataatg tcgcgaaggc gcttcgcctc ctcgaagtcc atcgccgccg cggcttcctc
     2221 catccgctgg tgcagatcgc cgatctcgtc gctcatcccg ccccaacgct
//
LOCUS       DASWNU010000548         6777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_490095, whole genome shotgun sequence.
ACCESSION   DASWNU010000548 DASWNU010000000
VERSION     DASWNU010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490095"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..864)
                     /locus_tag="VGB57_10805"
     CDS             complement(166..864)
                     /locus_tag="VGB57_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEY0131882.1"
                     /translation="MIYSRRQLLGAGLSAGFLATAAQGQTLAQIVAGLKDPAPKPLPV
                     APVHPRSLPAQPRVAVDGVIDPRLLARARASFERHRSQLRYTDVVGIADFSRPSNEHR
                     FFLLDTASGRVSNHLVAHGRGSDPVHSGLLQRFSNQIGSEASSQGGYRTGEYYHGKYG
                     RSLRLAGLDWSNSNAHTRAIVVHSAWYAEPEQLAAHGKLGRSEGCFALPYNSLQQVLA
                     RLGPGRFLYADKLA"
     gene            1057..2103
                     /locus_tag="VGB57_10810"
     CDS             1057..2103
                     /locus_tag="VGB57_10810"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0131883.1"
                     /translation="MAEFDRLELKPGLAPMEAKLVDSLPEGEGWQYEPKWDGFRCLVF
                     RDGPEVALMSKSGKPLGRYFPEVVETVLGMEADRFVLDGELVIPVDGVLSFDALQLRL
                     HPAESRVNRLALETPAQLMLFDLLFSKETRYVDAPLEVRRRSLEIIYAAEQHDDLLLS
                     PCTQDPDVARGWLDQVGAALDGVVAKRRDGRYEPGERAMLKIKAMRTADCVVGGFRYG
                     TNSREVASLLLGLYDDEGLLHHVGFTSSIPAGEKAALTERLEALVEAPGFTGDAPGGP
                     SRWSTERTGEWQPLRPELIAEVRYDHVTGRRFRHGTKFLRWRPDKSPEQCRMDQLQPE
                     AAADALAARLKAAS"
     gene            2100..2981
                     /gene="ligD"
                     /locus_tag="VGB57_10815"
     CDS             2100..2981
                     /gene="ligD"
                     /locus_tag="VGB57_10815"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0131884.1"
                     /translation="MKPERNVEGVRLSNPDKILYPEQGITKGQLADYYVAVAGRILPH
                     LAFRPVTMVRCPMGQETKCFYQRHAGSGVPEQLHEVTIPGFDEPYLYISDVAGLVAMV
                     QMGVLEMHPWGVKVEAPDRADRIVFDLDPAEGLGFEAVIEAAREVRDRLTALGLNSFV
                     KTTGGKGLHVVVPVEATAPWKEVKSFAKGVSAEMAADSPGKYLTKISKAEREGRIFID
                     YLRNDPTSTAVAPYSTRSRKGAPVAMPIRWDQVKAGLDPCDYTVETVPALVARQRSDP
                     WAEMLEVRQALPEGPRG"
     gene            3210..3761
                     /gene="msrA"
                     /locus_tag="VGB57_10820"
     CDS             3210..3761
                     /gene="msrA"
                     /locus_tag="VGB57_10820"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0131885.1"
                     /translation="MAEQQAILAGGCFWCTEAVFNDVIGVTRVESGYIGGDVPNPSYK
                     AVCGGNTGHAEAIRLTYDPDIISYDEILDLFFATHDPTQLNRQGNDVGTQYRSAIFPL
                     SPEQEAAVRRAIERAGADWPAPIVTTIEPASDWWPAEDYHQDYWAGEGQRNPYCLAII
                     PPKLKKLRKSFAHRVRSEAAGAA"
     gene            3933..4403
                     /locus_tag="VGB57_10825"
     CDS             3933..4403
                     /locus_tag="VGB57_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131886.1"
                     /translation="MKRYLTICGLAAVSMLLPACATVTRGTSQKYVIQSSPAEAQVAL
                     STGQKCTTPCKLKLKRKRGFTASFTKPGYQPLTAQVKSKFSGGGAAAGAGNLLMGGII
                     GGAIDGANGAMNNLTPNPLKVTLVEVGDTVNVGNAGDAPVVNTAPDGTQPNPQF"
     gene            complement(4458..5210)
                     /locus_tag="VGB57_10830"
     CDS             complement(4458..5210)
                     /locus_tag="VGB57_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEY0131887.1"
                     /translation="MPLALQAGLWGLVGGSALLIGALAGWFLPMPRRLVASIMAFGAG
                     VLISALSFDLMDQAWGRGGLWPVAAGFFGGAAFFTACNRLLAAWGARHRKRSDPKLRA
                     AHKQDNGAALALGALIDGIPESIVIGVSLLEGGVIGLVAVVAVFLSNLPEGLASAAGM
                     KAEGKSAGFVFLLWGAIALASGAAALGGYLAFDGVDPPAIAAVQAVAAGAILAMIVDT
                     MVPEAFEDTQDYTGLIAVTGFLAAFLLSKLGG"
     gene            complement(5210..6151)
                     /locus_tag="VGB57_10835"
     CDS             complement(5210..6151)
                     /locus_tag="VGB57_10835"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0131888.1"
                     /translation="MQFGADAVAEAARLIRSGEVVAVPTETVYGLAADATSSDAVDRI
                     YEAKGRPRFNPLIVHVASVDQAREIAEFADSASTLARLYWPGPLTMVLPLRRGHGLSS
                     ALLGRNETVALRMSRHPAMEALLAASGTPLAAPSANASGTISPTRAEHVVASLGGRIP
                     LVIDGGPTQAGLESTIVEVGPDRIRLLRPGPLDVHRISAMAKLAMEEPLGLDVGRLRA
                     PGQLASHYAPSKPLRLNAIAAEPDEWMIGFGAVAGDDNLSPSGDLDEAAARLFDALHR
                     SEGEGRPRIAVAPIPNEGIGLAINDRLGRAAAPRDRD"
     gene            6229..>6777
                     /locus_tag="VGB57_10840"
     CDS             6229..>6777
                     /locus_tag="VGB57_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0131889.1"
                     /translation="MTYTPPVADQRFVLRHIVRMDELAGHDAFAEATPDLVDAIIEGV
                     GEFAAGEFAPLNRVGDRIGAKWSEQGVTMPPGFIEAYRAYVEGGWGSLAGPPEQGGQG
                     LPLVLATVVMEDLGAANMAFSLVTMLSAGAVEALRHHGSPEMQAQWLPRLVTGEWTGT
                     MNLTEPQAGSDVGALKSRAEPIG"
BASE COUNT         1148 a         2225 c         2386 g         1018 t
ORIGIN      
        1 gatgaccgag cggcacctga tcgcctatgc gatcctgctc ttcttcctga tgagcgccgg
       61 cttcatctgg cgtctgcgac gcaggcgcgc ccgccgggcg cccaaacgcc acctgcgcat
      121 cgacctgctg cccgagaccg ccgaggaggc ggcggtcgga gagccttaag cgagcttgtc
      181 ggcgtagagg aagcggcccg gcccgaggcg ggcgaggacc tgctggaggc tgttgtaggg
      241 gagggcgaag cagccctccg agcggccgag cttgccgtgg gcggcgagct gctcgggctc
      301 ggcataccag gccgagtgga cgacgatggc gcgggtgtgg gcgttcgagt tgctccagtc
      361 gaggccggcg agccgaagcg agcgtccgta tttgccgtga taatattcgc cggtgcggta
      421 gccgccctgc gacgaggctt cggagccgat ctgattggag aagcgttgga gcagtccgct
      481 atggaccgga tcggagccgc ggccgtgggc gacgagatga ttgctgaccc gcccggacgc
      541 ggtgtcgagc aggaagaagc gatgctcgtt cgaggggcgg ctgaaatcgg cgatcccgac
      601 cacgtcggtg tagcgaagct gcgagcggtg gcgctcgaac gaggcgcggg cgcgggcgag
      661 caggcgcggg tcgatgactc cgtcgacggc gacccgcggc tgggcgggaa ggctgcgagg
      721 atgcaccgga gccaccggaa gcggcttcgg cgccggatcc ttgaggcctg cgacgatctg
      781 agccagcgtc tgcccttggg ccgcggtggc gagaaatccg gccgaaagtc cggccccaag
      841 caattgcctg cgcgagtaga tcaagatttt cccctcagct ctggagtcct agttatagct
      901 tttgtcctta agttccagtg aactgcagcc gcttcatccc gtcggtgcag cgactcgtcg
      961 caacattacg gctccggttg gttaccgccg cgtaacgtcg tcggcgaagc gtgcgtggaa
     1021 cccggcaggc cgtggaagcg tctctacccg cgagagatgg ccgaattcga tcgccttgaa
     1081 ttgaagcccg ggcttgcgcc gatggaggcg aagctggtcg attcgctgcc cgagggcgaa
     1141 ggctggcaat atgagcccaa atgggacggc ttccgctgcc tcgtcttccg cgacgggccc
     1201 gaagtggctt tgatgtcaaa gtcgggaaag ccgctcgggc gctattttcc ggaggtggtg
     1261 gagacggtgc tcgggatgga ggcggaccgc ttcgtcctcg acggcgagct ggtcattccg
     1321 gtcgacggag tgctttcctt cgacgccctc cagctccgcc tccatccggc cgagagccgg
     1381 gtcaaccggc tggcgctcga gaccccggcg cagctgatgc tgttcgacct gctgttctcg
     1441 aaggagacgc gctatgtcga cgcgcccctc gaagtgaggc gccgctcgct cgagatcatc
     1501 tacgcggcgg agcagcatga cgatcttctg ctttcgccct gcacccagga tccggacgtc
     1561 gcccggggct ggctcgatca ggttggagcg gcgctcgatg gagtggtcgc caagcgccgc
     1621 gacggcagat acgagccggg cgagcgggcg atgctgaaga tcaaggcgat gcggaccgcc
     1681 gactgcgtcg tcggcggctt ccgctacggc accaacagcc gcgaggtcgc ctcgctcctg
     1741 ctcggtctgt acgacgacga aggcctgctc caccatgtcg gcttcacctc ctcgatcccc
     1801 gccggagaga aagcggccct gacggagcgg ctcgaggctc tggtcgaggc acccggcttc
     1861 accggcgatg cgccgggcgg gccgagccgc tggtcgaccg agcgcaccgg cgaatggcag
     1921 ccattgaggc ccgagctgat cgccgaggtg cgctacgacc atgtcaccgg ccggcgcttc
     1981 cgccacggca ccaaattcct gcgctggcgg cccgacaagt cgccggagca atgccggatg
     2041 gaccagctcc agcccgaagc ggccgcggac gctctggccg cccggctgaa ggcggcgtcg
     2101 tgaagcccga gcggaacgtc gagggggtgc ggctgagcaa tcccgacaag atcctctatc
     2161 ccgagcaggg catcaccaag ggccagctcg ccgattatta tgtcgccgtc gccggccgga
     2221 tcctgccgca tctggcgttc cgcccggtta cgatggtgcg ctgcccgatg ggccaggaga
     2281 ccaagtgctt ctaccagcgc cacgccgggt ccggcgttcc ggagcagctt cacgaggtga
     2341 ccatcccggg cttcgacgag ccctatctct acatctccga cgtggccggg ctggtcgcga
     2401 tggtccagat gggggtgctg gaaatgcatc catggggggt gaaggtggag gcgcccgacc
     2461 gcgccgaccg catcgtcttc gacctcgatc ccgccgaggg tctcggcttc gaggcggtga
     2521 tcgaggcggc gagggaggtt cgcgaccggc tgacggcgct cggcctcaac agcttcgtca
     2581 agacgaccgg cggcaagggc ctccacgtcg tcgtccccgt cgaggcgacg gcgccgtgga
     2641 aggaagtgaa gagcttcgcc aaaggcgtgt cggcggagat ggcggcggac tcgcccggca
     2701 aatatctgac caagatctcg aaggcggaac gggaagggcg gatcttcatc gactatctgc
     2761 gcaacgaccc cacctcgacc gccgtcgccc cttattcgac ccgctcgcgc aagggcgccc
     2821 cggtggcgat gccgatccgg tgggaccagg tgaaggcggg gctcgatccg tgcgattata
     2881 ccgtggagac ggtgccggcg ctggtggcga ggcagcgcag cgatccttgg gcggagatgc
     2941 tcgaggtgag gcaggccctg ccggaagggc cgaggggata atctgcctcc ccatgcgcag
     3001 cgtggggagg gggaccaccc gaagggtggt ggaggggtag aaggcgcgga tcaaagacgg
     3061 ttgcgcggga accgcccgat tcccgcgcca gctacccctc caccatgctt cgcatggtcc
     3121 ccctccccac cgcttcgcgg cagggaggca aagacttgcc gtcaccgccg cgcctcctat
     3181 ctcccaaaga catcaaaagg agtgacgaca tggccgaaca gcaggcgatc ctggcgggcg
     3241 ggtgcttctg gtgcaccgag gccgtgttca acgacgtgat cggagtgacc agggtcgaga
     3301 gcggctatat cgggggcgat gtgccgaacc cgagctacaa ggccgtgtgt ggcggcaata
     3361 ccggccatgc cgaggcgatc cggctgacct acgatccgga catcatctcc tacgacgaga
     3421 ttctcgacct cttcttcgcc acccacgatc ccacccagct caaccgccag ggcaacgatg
     3481 tcggcaccca atatcgctcg gcgatcttcc cgctttcgcc ggagcaggaa gcggccgtgc
     3541 gccgcgccat cgagcgagcc ggggccgact ggccggcgcc gatcgtgacc accatagagc
     3601 ccgcctccga ctggtggccc gccgaggatt accatcagga ttattgggcg ggcgaaggcc
     3661 agcgcaatcc ctattgcctc gcaatcatcc cgcccaagct caagaagctg cgcaagagct
     3721 tcgcccaccg ggtgaggagc gaggcggcag gggcggcctg aaccgcggcg gtcccgtcag
     3781 cacgccaact tcacggcggc gcgcgcccgc gccttcctcc gttcgcattc gtctttccga
     3841 taaaatattc gagaaacgat tgcggggcaa agacttctgt gtaacgtcga catgatcggc
     3901 ggcgggggct gccgattatg aggggacaca tcgtgaaacg ctatcttacc atttgcggcc
     3961 tcgcggccgt ttccatgctg ctgcctgcct gcgccaccgt cacgcgcggc acgtcgcaga
     4021 aatacgtgat ccaatcgtcg ccggcggagg cgcaggtggc gctgtccacc ggccagaagt
     4081 gcacgactcc gtgcaagctc aagctgaagc gcaagcgcgg cttcaccgcc agcttcacca
     4141 agcccggcta tcagccgctg acggcccagg ttaagagcaa gttctcgggc ggcggcgcgg
     4201 ccgccggcgc gggcaacctc ctgatgggcg gaatcatcgg tggagcgatc gacggcgcga
     4261 acggcgccat gaacaatctg acacccaatc cgctgaaggt aacgctcgtc gaggtcggcg
     4321 acaccgtcaa tgtcggcaac gccggcgacg ctccggtcgt caacacggcc cccgacggaa
     4381 ctcagccgaa cccgcaattc tagcattcgg ctcgaaattg aatggaaaag ggtcggcttc
     4441 ggccgcccct ttttcgttca gccgccgagc ttcgaaagca ggaaggcggc gaggaagccg
     4501 gtgacggcga tcaggccggt ataatcctgg gtgtcctcga acgcctcggg aaccatcgtg
     4561 tcgacgatca tcgccaggat cgcgccggcg gcgacggcct ggaccgcggc gatcgcgggg
     4621 ggatcgaccc cgtcgaaggc gagatagccg cccagcgccg cagcgccgga ggcgagggcg
     4681 atcgcgcccc agagcaggaa gacgaagccg gcgctcttac cctcggcctt catgcccgcc
     4741 gcgctggcga ggccctccgg caggttggag aggaagacgg cgacgacggc gacgaggccg
     4801 atcactccgc cctcgagcag gctgacgccg atgacgatcg attccgggat tccgtcgatc
     4861 agcgcgccga gggcgagggc ggcgccattg tcctgcttgt gggcggcccg aagcttcgga
     4921 tcgctgcgct tgcggtgtcg cgcgccccag gcggcgagca gccgattgca ggcggtgaag
     4981 aaagcggcgc cgccgaagaa gccggcggcg accggccaca agccgccccg gccccacgcc
     5041 tggtccatca gatcgaagga cagcgccgag atcaggaccc cggcgccgaa cgccatgatc
     5101 gacgcgacga gccggcgcgg catcggcagg aaccagccgg cgagcgctcc gatcagcagg
     5161 gccgagccgc cgacgaggcc ccagaggccc gcctgcagcg ccagcggcat cagtcgcggt
     5221 ccctcggcgc cgccgcccgg ccgaggcggt cgttgatggc gagtccgatg ccctcgttcg
     5281 gaatgggcgc gacggctatg cgtgggcgtc cctccccttc ggagcggtgg agagcgtcga
     5341 acaggcgggc ggcggcttca tcgaggtcgc cggaagggga gaggttgtcg tcgcccgcga
     5401 cggcgccgaa gccgatcatc cattcatcgg gctcggcggc gatggcgttc agacgcagag
     5461 gcttcgaggg ggcgtaatgg ctggcaagct ggcccggggc gcgcagtcga ccgacgtcca
     5521 gcccgagcgg ctcctccatc gcaagcttcg ccatcgcact gattcgatga acgtcgagag
     5581 gcccgggacg aagcagccgg atgcggtccg gccccacctc cacaattgtg gattcgaggc
     5641 ccgcctgggt cgggccgccg tctatgacca gggggatccg gccgccgagg ctggcaacta
     5701 catgctcggc gcgggtgggg ctgatcgtcc cgctggcatt ggcggagggt gccgcgagcg
     5761 gagtcccgga ggcggcgagc aacgcctcca tcgccgggtg gcgggacatc ctgagcgcga
     5821 ccgtctcgtt gcggccgagg agcgcgctgc tcaggccgtg acccctcctc agcggaagca
     5881 ccatggtgag cggacccggc cagtagagac gcgcaagggt cgatgcagag tcggcgaact
     5941 cggcgatctc cctggcctgg tcgaccgagg cgacatgaac gatgagcgga ttgaagcgcg
     6001 gcctgccctt ggcctcgtag attcggtcga cggcatcgga gctggtcgca tcggcagcaa
     6061 ggccgtagac ggtctcggtc ggcaccgcga cgacctcgcc cgaccggatc aggcgcgccg
     6121 cctccgccac ggcatcggcg ccgaactgca tgacacgggt gttttgcgcc ttcaccgccc
     6181 cgcgctatag cccccgttcc agcagcgcaa cggcccgcga ggagccccat gacctacacc
     6241 cctcccgtcg ccgaccagcg cttcgtgctc aggcatatcg tccggatgga cgaactcgcc
     6301 ggccacgatg cgttcgccga ggcgacgccc gacctggtcg acgccataat cgaaggggtg
     6361 ggcgagttcg ccgcgggcga gttcgcgccg ctcaatcggg tcggcgaccg gatcggcgcg
     6421 aaatggtcgg agcagggggt gacgatgccg cccggcttca tcgaggccta tcgcgcctat
     6481 gtcgagggcg gctggggcag cctggcgggt ccgccggagc agggcgggca ggggcttccg
     6541 ctcgtcctgg cgacggtggt gatggaggat ctgggcgccg cgaacatggc cttctcgctg
     6601 gtgacgatgc tcagcgccgg agcggtcgag gcgcttcgcc accacggctc gccggagatg
     6661 caggcgcagt ggctgcccag gctcgtcacc ggcgaatgga ccgggacgat gaacctcacc
     6721 gagccgcagg caggctcgga cgtcggcgcg ctcaagtcca gggccgagcc gatcggc
//
LOCUS       DASWNU010000549         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_306472, whole genome shotgun sequence.
ACCESSION   DASWNU010000549 DASWNU010000000
VERSION     DASWNU010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306472"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VGB57_10845"
     CDS             complement(<1..1033)
                     /locus_tag="VGB57_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131890.1"
                     /translation="MAETVRRVGGEFGVNSVTQGRQGDQAVARLPNGGFVVAWTDSSR
                     IGADRDESAIKAQIFDAGGTKIGGEFTVNAFIRGGQTWPSVVALPDGSFLVTWTTGWP
                     DPADGGDGSGSAVKGRLFNPDGNPLGDEFPINNEAPLDQDFSFAYASNSAAFTVMWQS
                     DTPSGEADIKSRRFDSTGAQRESEIYVTETFAGSEVEPAGVRLYPGRDVIVWTDGNSS
                     GGDIKARFISSQGFGSAGRGTGPEFLVNSVLDGAQGQPDVAALPNGGFVVTWASLEGD
                     LYLAKAQVFAADGSRAGEEILLSQAGHSIASPTVVEFGTGDFFIAWVDLDTTVTPPGG
                     AAVLGQYFNA"
     gene            complement(1245..>1545)
                     /locus_tag="VGB57_10850"
     CDS             complement(1245..>1545)
                     /locus_tag="VGB57_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131891.1"
                     /translation="YQNVAESSSGSMDHILDFTPGTDKVELDRIDANALVAGNQAFSW
                     IGSSAFTGSAGQLRAYEQGGTWFVEGDVNGDGSADLVIALTLQGATPLGAGDFLL"
BASE COUNT          242 a          521 c          527 g          255 t
ORIGIN      
        1 gggcgttgaa atactggccg agcacggccg ccccgccggg cggagtcacg gtcgtatcca
       61 ggtccaccca ggcgatgaag aagtcgcccg tgccgaactc gacgacggtc ggcgaagcga
      121 tgctgtggcc agcctgcgac aacaggattt cctcacctgc cctggagccg tcggcggcga
      181 agacttgcgc cttggcgagg tacaagtcgc cttcgaggga cgcccaggtg acgacgaagc
      241 cgccattggg aagtgccgcc acgtccggct gtccctgggc gccgtcgagg acgctgttga
      301 cgaggaattc ggggccggtc ccccggccgg ccgagccgaa gccctgcgag ctgatgaagc
      361 gcgccttgat gtcgccgccg ctgctattgc cgtccgtcca gacgatcacg tcccggccgg
      421 ggtaaagtct gaccccggcg ggctcgacct cggatccggc gaacgtctcg gtgacgtaaa
      481 tttcggattc cctctgggcg ccggtcgaat cgaagcggcg actcttgatg tccgcctccc
      541 cgctcggggt gtcgctctgc cacatcaccg tgaaggccgc cgaattggac gcgtaggcga
      601 acgaaaagtc ctgatcgagc ggagcctcgt tgttgatcgg gaactcgtcg ccgagggggt
      661 tgccgtccgg gttgaacagg cggcccttga ccgccgagcc cgagccgtcg cctccgtcgg
      721 cgggatccgg ccagcctgtc gtccaggtga cgaggaagct gccgtcgggc agcgccacca
      781 cgctcggcca ggtctggccg ccccggatga acgcgttgac cgtgaactcg ccgccgatct
      841 tggtgccgcc ggcgtcgaaa atctgcgcct tgatcgcgct ttcatcccgg tcggcgccga
      901 tccggctgct gtcggtccag gcgacgacga agccgccgtt gggcagccgg gcgacggcct
      961 ggtcgccctg gcggccctgg gtaacagagt tgacgccgaa ttcgccgccg acgcgccgca
     1021 cggtttccgc cattgtcttt ccccgcttcg atggccgaac gtgttaccgc tcggcggaag
     1081 cgccgcaaag gcttatcgga aaaggattaa tccataacgg acaggccgga tcgggaccgg
     1141 ctcggccgat cccttcccgt catcccggcg aaagccggga cctcggccag aatgaggctc
     1201 tgccaggtga gccgaggtcc cggcttgcgc cgggacgacg gtggctagag caggaagtcc
     1261 cccgcaccga ggggtgtggc tccctgcagc gtcagcgcga tgacgaggtc ggcgctgccg
     1321 tcgccgttca cgtcgccctc gacgaaccag gtgccgcctt gctcataggc tcgaagctgc
     1381 cccgccgagc cggtgaaggc gctggagccg atccagctga acgcctggtt gcccgcgacg
     1441 agcgcgttgg cgtcgatccg gtcgagctcg accttgtccg tccccggggt gaagtcgagg
     1501 atatggtcca tcgaccccga gctcgactcc gcgacgttct ggtag
//
LOCUS       DASWNU010000550         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_21704, whole genome shotgun sequence.
ACCESSION   DASWNU010000550 DASWNU010000000
VERSION     DASWNU010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21704"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="VGB57_10855"
     CDS             complement(<1..1649)
                     /locus_tag="VGB57_10855"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY0131892.1"
                     /translation="MSFLIRTVSHSAEGREIVRARRVEGERLTVGRDPACDVHLTDLA
                     VALRHLTVERDGGRLRATVEPGLSVELNGRKASGGAIELATGGDILLGGHVLRFMPAA
                     AGSEELAVAVERTGDSEVRLGKSAERLFSLASVLPGKRPLAWLLALLVLAVGLAWPIK
                     AYYDRRERAETFARFHADELWSSGSLSRAHAGLKDNCTACHVKPFEAVRDSACKTCHT
                     AVHDHADPFRLARARPDLGRWRRIELAFKEKFDLPPGRCVECHTEHEGPQQMPATAQR
                     FCADCHGDLKKKLPDTPLPDAGDFGRSHPAFSPVLIAGWNGERPRLRRLSLARRPQEE
                     SGLKFPHALHLSRTSGVAQMARRLGSDHGFGQALECKDCHDPAPDGARFQPVDMEQDC
                     AMCHDLAFDRSGGTTRTLRHGEPAQVVAELRDFYRGRGSARPSVLTPAARRRPGDAPD
                     LRNQIQFARGPGGAEQAIRRVFSPGGACFDCHRVDAPPGGSLNFRIHPVAFPVRYLHK
                     GWFDHRPHSTQSCSSCHSAGRSNAASDLLIPDVTTCRTCHGG"
     gene            complement(1656..2525)
                     /locus_tag="VGB57_10860"
     CDS             complement(1656..2525)
                     /locus_tag="VGB57_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131893.1"
                     /translation="MPARSIPRTDLGASHEGFLRHRNFLWLKVALTLSVLALAGYTLI
                     DVQPRPNGGSAYGYALGTAATLLILWLTLLGLRKRAMTRGRWSLKAWTSAHVYLGLSL
                     VVLATLHTGFQFGWNVHTLAYALMILVVGSGVYGIAVYATLPPALGASRSDMTRPQMI
                     DALVKLDRQLQVAAQPLGHDEAGIVQFSLADDPFRGGLLRRLSGRHRNCRTARALAET
                     RRRLAAAAGEQAQALESVAGLLERKAAALARIRGHLRIKALLEIWLYVHVPVTFALIA
                     ALASHIVSVFFYW"
     gene            complement(2612..>3471)
                     /locus_tag="VGB57_10865"
     CDS             complement(2612..>3471)
                     /locus_tag="VGB57_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY0131894.1"
                     /translation="AGGRPVFLSYLPAGSYVGEMALLEGGRRTATVRAAVKSEVIRLD
                     GDSFRRLLDRKPELLARARSDMASRRRLNDFIEAGKDGFDGVVDMYSSVASFLVENGI
                     GEATDVLLIDETLCVGCDNCERACADSHEGLSRLDREAGKTYAHLHVPTSCRHCEHPH
                     CMADCPPNAIHRGPDGEVFIDDTCIGCGNCQRNCPYGVIRMEKVPPKKPPLLAWLLAG
                     LGPGPGEPPRKWIDRRVSADTPKKAVKCDMCAGISGGPACVRACPTGAAIRVAPEQFL
                     TVARLEREG"
BASE COUNT          517 a         1084 c         1386 g          484 t
ORIGIN      
        1 ccgccatggc aggtccggca ggtcgtgacg tctgggatca gcaggtcgga ggcggcattg
       61 gaccgcccgg cggagtggca gctcgagcag ctctgggtgg agtgggggcg gtggtcgaac
      121 cagcccttgt ggagatagcg gaccgggaag gcgaccggat ggatcctgaa gttcagcgag
      181 ccgccgggcg gcgcgtcgac ccggtggcag tcgaagcagg cgccgccggg cgagaagact
      241 cgcctgatcg cctgctcggc gccgcccggg ccgcgggcga actggatttg gttgcgcagg
      301 tccggagcgt cgccgggacg ccgccgggcc gccggggtca gcaccgaggg acgcgccgag
      361 cctcggccgc ggtagaagtc gcgaagctcg gcgacgacct gggccggctc gccgtggcga
      421 agggttcggg tggtgccgcc gcttcggtcg aaggcgaggt cgtggcacat ggcgcaatcc
      481 tgctccatgt cgaccggctg gaagcgggcg ccgtcggggg cgggatcgtg gcagtccttg
      541 cattccagcg cctggccgaa gccgtggtcg ctgcccagcc gcctggccat ttgcgcgacc
      601 ccgctggtcc gcgacaggtg cagggcgtgg gggaatttga ggccgctctc ctcttgcggc
      661 cgccgcgcca gcgagagacg gcggagccgg ggccgctcgc cgttccagcc ggcgatcagg
      721 acgggcgaga aggcggggtg ggaacggccg aaatcgccgg catcgggcag aggcgtgtcg
      781 gggagcttct tcttcaggtc gccgtggcaa tcggcgcaga aacgctgcgc cgtcgccggc
      841 atctgctgcg gcccttcatg ctcggtatgg cattcgacgc agcggcccgg aggcaagtcg
      901 aatttctcct tgaaggcgag ctcgatccgg cgccagcggc caaggtcggg gcgggcgcgg
      961 gcgaggcgga aggggtcggc atggtcgtgg acggcggtgt ggcaggtctt gcaggcggaa
     1021 tcgcgcaccg cctcgaacgg cttgacgtgg caggcggtgc aattgtcctt gaggccggca
     1081 tgggcccggg agaggctgcc cgaggaccat agctcgtcgg cgtggaatcg ggcgaaggtc
     1141 tcggcgcgct cgcggcggtc gtaataggcc ttgatcggcc aggcgaggcc gacggccagg
     1201 acgagcagtg cgagcagcca ggcgaggggg cgcttgccgg ggagcaccga ggcgaggctg
     1261 aacaggcgct cggcgctctt cccgagccgg acctcgctgt cgccggtgcg ctcgacagcg
     1321 accgcgagct cctccgagcc ggccgcagcg ggcatgaagc gcaggacatg gccgccgagg
     1381 aggatgtcgc cgccggtggc gagctcgatg gctccgcccg aggccttgcg accgttcagc
     1441 tccaccgaca ggcccggttc caccgtcgcc cggaggcggc ccccgtcgcg ctcgacggtc
     1501 aggtggcgaa gggcgacggc gaggtcggtg aggtggacgt cgcaggcggg gtcgcggccg
     1561 acggtgaggc gctccccctc cacccggcgg gcgcggacga tctcgcggcc ttcggcgctg
     1621 tgcgagacgg tgcggatcag gaagctcaca agccgctacc agtagaagaa gacgctgacg
     1681 atatgggagg cgagcgcggc gatgagggcg aaggttacgg gcacgtggac gtagagccag
     1741 atttcgagaa gcgccttgat tcgaaggtgg ccgcggatcc gggccagcgc cgcggccttg
     1801 cgttcgagca agccggcgac gctttccagg gcctgggcct gctcgccggc ggcggcggcg
     1861 agccggcggc gggtctcggc gagggcccgg gcggttcggc aattgcgatg gcggcccgac
     1921 aggcggcgca agaggccgcc gcggaaagga tcgtcggcga gcgagaactg gacgatcccc
     1981 gcctcatcgt ggccgagcgg ctgggcggcg acctgaagct ggcgatcgag cttgaccagc
     2041 gcatcgatca tctgcggccg ggtcatgtcg gagcggctcg cgccgagggc cggaggcaaa
     2101 gtggcgtaga cggcgatccc gtagacgccc gagccgacga ccaggatcat cagggcgtag
     2161 gccagggtgt ggacgttcca gccgaactgg aagccggtgt ggagggtggc gaggacgacc
     2221 agcgacaggc cgagatagac gtgggcgctg gtccaggcct tgagcgacca gcggccccgg
     2281 gtcatcgccc gcttcctcag gccgagcagg gtcagccaga ggatgaggag ggtggcggcg
     2341 gtgccgaggg cgtagccgta ggcgctgccg ccgttggggc ggggctggac gtcgatcagg
     2401 gtgtagccgg cgagggcgag gacggagagc gtcagggcga ccttgagcca gaggaaattg
     2461 cgatggcgga gaaagccttc atggcttgcg ccgaggtcgg ttcgggggat cgagcgggcg
     2521 ggcatctaca cccctgctcc ccggcgaagg ccggggtcca gtcgggcgtt gaaactgggc
     2581 cccggccttc gccggggagc aggggcgcgt ctcatccctc ccgctccagc ctggcgacgg
     2641 tcaggaactg ctccggcgca acgcggatcg ccgcccccgt ggggcaggcc cggacgcagg
     2701 cggggccgcc acttatgccc gcgcacatgt cgcatttgac cgctttcttg ggcgtgtcgg
     2761 ccgagaccct cctgtcgatc cacttcctgg gcggctcgcc cggccccggt ccgagcccgg
     2821 cgagcagcca ggcgaggaga ggcggcttct tcggcggcac cttttccatc cggatgaccc
     2881 cgtaggggca gttgcgctgg cagtttccgc agccgatgca ggtgtcgtcg atgaagacct
     2941 cgccgtccgg gccgcggtgg atggcgttgg gcgggcaatc ggccatgcag tgcggatgct
     3001 cgcaatggcg gcacgaggtc ggcacgtgga gatgggcgta ggtcttgccc gcctcgcggt
     3061 cgagccggga gaggccttcg tggctgtcgg cgcaggcgcg ctcgcaattg tcgcagccga
     3121 cgcagagggt ctcgtcgatg aggaggacgt cggtggcctc gccgatgccg ttctcgacga
     3181 ggaagctcgc cacggaggaa tacatgtcga cgaccccgtc gaagccgtct ttcccggcct
     3241 cgatgaagtc gttcaagcgc cgccgcgacg ccatgtccga gcgggcccgg gcgagcagct
     3301 ccggcttgcg gtcgaggagg cggcggaacg agtcgccgtc gaggcggatc acctccgact
     3361 tgaccgcggc gcgaacggtc gcggttcggc ggccgccttc gagcagggcc atctcgccga
     3421 cataggatcc ggccgggaga taggagagga agacgggcct tccgccggct g
//
LOCUS       DASWNU010000551         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_437945, whole genome shotgun sequence.
ACCESSION   DASWNU010000551 DASWNU010000000
VERSION     DASWNU010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437945"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGB57_10870"
     CDS             complement(<1..633)
                     /locus_tag="VGB57_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131895.1"
                     /translation="MSTLFPNSAGMQRRRLAAAVQARDPAEAADALRRLAGMGAVLSE
                     AGQAQAAQLVGAERMAPIAAQFAANAVPVAASRLHASIPAGHDLVEGVVWDSAAGRLY
                     ATTVVGRALLAVDPVGTSVAVPPGPASLFGAAYDPDRKRIWIASASVAQTPKVEFAFV
                     GLLVFDPVRPGEPRRIPAPAGMTANPGDVTIARDGTAYASDGLNGALYRCR"
     gene            885..1361
                     /locus_tag="VGB57_10875"
     CDS             885..1361
                     /locus_tag="VGB57_10875"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY0131896.1"
                     /translation="MRLVGWTIAIAAAGMAIPAQAQMVSARKPEALVRALQAAGYSAK
                     LEKDATGDPMIKSAVNGHPFRVVFYGCKANKDCATITFAAGYDKKGATSLDSINDWNR
                     KNRFGRAYLDDESDPIIGMDVDLDDGGMSSALFTDNLEFWTAVTGAFQKHIGWDKE"
     gene            complement(1499..>2131)
                     /locus_tag="VGB57_10880"
     CDS             complement(1499..>2131)
                     /locus_tag="VGB57_10880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0131897.1"
                     /translation="PDWRLTLEEGGSIEGDPDKPRFIVDPLDGTSNFLHGLPHFAISI
                     AVEDPRGAGGRPEITQALIYQPLTDESFWAEKGRGAWLNERRLRVSARRDLADALIAT
                     GVPWKGHGHLDRFQKILDAVGPEVAGIRRFGSAALDLAWVAAGRFDGFWEEDLKLWDV
                     AAGILLVREAGGFVTDFRGADSALEKGQVLAANDQLHSRLHKLVAGALRA"
BASE COUNT          356 a          715 c          758 g          302 t
ORIGIN      
        1 cctgcagcgg tagagcgcgc cgttcaggcc gtcgctggcg taggcggttc cgtccctggc
       61 gatcgtgaca tcgcccggat tggccgtcat ccccgcgggc gccggaatcc ggcgaggctc
      121 gcccgggcgc accggatcga agaccagcag gccgacgaag gcgaattcga ctttcggagt
      181 ctgcgcgacg gaggcggaag cgatccagat gcgcttgcgg tccggatcgt aggcggcgcc
      241 gaacaggctt gccgggcctg gcgggaccgc caccgaggtg ccgaccgggt cgacggccag
      301 caaggcgcgg cccaccaccg tcgtcgcgta gaggcgaccg gcggcgctgt cccaaacgac
      361 accttcgacg aggtcgtgcc cggccggaat ggaggcgtgg agccggctgg ccgcgaccgg
      421 caccgcattg gcggcgaatt gcgccgcgat cggcgccatc cgttccgcgc cgacgagttg
      481 cgccgcttgc gcctgtcccg cttcggacag gaccgcgccc atcccggcga gccgtcgaag
      541 cgcgtcggca gcctcggccg ggtctctcgc ctgcacggcg gccgcgagcc ggcgccgttg
      601 catcccggcg ctgtttggga acagggtcga cacgcgggcg gcctgggccg gcgacgcgaa
      661 ttcccggacc gctgggggcc agtcgacgag cgcccgttgc cgaacctggg gcgaagcggc
      721 gcagccgcct gccagcagcg caaagagaat cgcgattacc cccctcttca tgcctcgctt
      781 atggcggttt ccggaacagg cgcaaaatgg cctgcgacgg ggacggttga cagcttcgcc
      841 ggcgcctcgc agcttggcgc gggttgaacg ggggagatag gaagatgcgg cttgtcggct
      901 ggacgatagc gatcgcggcg gcggggatgg cgattcccgc ccaagcgcag atggtcagcg
      961 ccaggaagcc cgaggccctg gtccgggcgc tccaggccgc cggctattcc gcgaagctgg
     1021 agaaggacgc gaccggcgat ccgatgatca agagcgcggt gaacggccac cccttccggg
     1081 tcgtcttcta cggctgcaag gcgaacaagg actgcgccac gatcaccttc gccgccggct
     1141 atgacaagaa gggcgcgacc agcctggact cgatcaacga ctggaaccgg aagaaccgct
     1201 tcggccgcgc ctatctcgac gacgagagcg atccgatcat cggcatggac gtcgatctcg
     1261 acgacggcgg aatgtcctcc gccttgttca ccgacaatct ggaattctgg acggcggtga
     1321 ccggcgcctt ccagaaacat atcggctggg acaaggaata ggctctacaa aattcctccc
     1381 ccggatttcc gggggagggg gaccgcgcga agcgcggtgg aggggttctt cagactcagc
     1441 aagaacccct ccaccaggct tcgcctggtc cccctccccg cgaaatcgcg gggaggattt
     1501 aagcgcgcaa agcccccgcc accagcttgt ggagccggga atggagctgg tcgttggcgg
     1561 ccagaacctg gcccttctcg agcgccgagt cggcgccgcg gaagtcggtg acgaaaccgc
     1621 ccgcctcgcg gacgagcagg atcccggcgg cgacgtccca gagcttgagg tcctcctccc
     1681 agaaaccgtc gaaacggccg gccgcgaccc aggcgaggtc cagtgcggcc gagccgaagc
     1741 ggcggatacc ggccacttcg gggccgactg cgtcgaggat cttctggaag cggtcgagat
     1801 ggccgtggcc cttccacggc actccggtgg cgatcagtgc gtcggcgaga tcgcggcggg
     1861 cggagacccg aagccggcgc tcgttgagcc aggcgcccct gcccttctcc gcccagaagc
     1921 tctcgtcggt gagcggctgg tagatcaggg cctgggtgat ttcgggccgc ccgcccgcgc
     1981 cgcgcggatc ctcgaccgcg atcgagatgg cgaaatgggg aaggccgtgg aggaagttcg
     2041 aagtgccgtc gagcggatcg acgatgaagc gcggcttgtc cggatcgccc tcgatcgagc
     2101 ccccctcctc gagggtcagc cgccagtcgg g
//
LOCUS       DASWNU010000552         7550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_281285, whole genome shotgun sequence.
ACCESSION   DASWNU010000552 DASWNU010000000
VERSION     DASWNU010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281285"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1532
                     /gene="pstC"
                     /locus_tag="VGB57_10885"
     CDS             150..1532
                     /gene="pstC"
                     /locus_tag="VGB57_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0131898.1"
                     /translation="MTPLALFIAILGLALLGWLSARARAMAFAPPGGPRLHSLPGYHG
                     WYVAIWAALPALLFLLLWVPLSDSLVMDSVLGSPAAANLPPFEMQREAILQETRELAE
                     GLRQSSFHPEARALAPVYDMALSRYRLIGTIAALLLAFAGGAFAYSRIGPAFRARTRV
                     ERLVMLALLLASLIAILTTLGIVLSLLFESLRFFSMVSPIEFLFGSTWSPQTAMRADQ
                     AGSSGAFGAIPLFWGTVFIGAIIAMIVAIPLGLMSAIYLTQYARPAVRRWMKPLLEML
                     AGVPTVVYGYFAALTVAPAVRDFGRSLGIPASSESALAAGLVMGIMIIPFVSSMADDS
                     LTAVPQSMRDGSLAMGATTSETIRKVLVPAALPGIVGGVLLAVSRAIGETMIVVMAAG
                     LSANLTANPFASVTTVTTQIVQLLTGDQEFDSPKTLAAFALGLVLFIVTLLLNIVALR
                     VVKKYREAYD"
     gene            1525..2598
                     /gene="pstA"
                     /locus_tag="VGB57_10890"
     CDS             1525..2598
                     /gene="pstA"
                     /locus_tag="VGB57_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY0131899.1"
                     /translation="MTDPSRLKRRYAAERRFRLFGLAAVLVSAAFLAFLLGTMAVRGA
                     GGFVETQIRVDPAVPREVALASELAARNVEPGAGPVWLPASAEIDAAAKGRGSDEAGA
                     AYAQLREQGRARTRFNLTFLTSADATDPAAVGVWGAFKGSHLTMLVTLAIAFPVGVLS
                     AIYLEEYAPRNRWTDLIEVSINNLAAVPSIIFGLLGLAVFLNTMHMPRSAPLVGGLTL
                     ALMTMPVIVIAGRNAIKSVPPSIRDAALGVGASRIQVVYHHVLPLALPGILTGTIIGM
                     ARALGETAPLLMIGMRAFISTPPGGFADPATVLPVQIFLWSDEVSRGFVEKTSAAIIV
                     LLVFLMAMNGIAIYLRNKFERRW"
     gene            2604..3416
                     /gene="pstB"
                     /locus_tag="VGB57_10895"
     CDS             2604..3416
                     /gene="pstB"
                     /locus_tag="VGB57_10895"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY0131900.1"
                     /translation="MVDEENRAEAHAVPATAGSAPKMHARGVNVWYGEKQAIKQVSIE
                     VRQEHVTAFIGPSGCGKSTFLRTLNRMNDTIPSARITGEIGLDGEDIYASGMDVVQLR
                     ARVGMVFQKPNPFPKSIYENVAYGPRIHGLAAGRQELDAIVERSLKRAGLWEEVKDRL
                     AESGTALSGGQQQRLCIARAIAVDPEVILMDEPCSALDPIATAKIEELIHELRGRYAI
                     VIVTHNMQQAARVSQRTAFFHLGELLEYGETSQIFTNPRVDRTKDYITGRYG"
     gene            3428..4129
                     /gene="phoU"
                     /locus_tag="VGB57_10900"
     CDS             3428..4129
                     /gene="phoU"
                     /locus_tag="VGB57_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY0131901.1"
                     /translation="MATGHTLKAFDDDLNQLRASIAEMGGLAEAAIAGSMRALAGRDS
                     EGARQVVEGDRRIDAFEAEIERLAVQIIALRAPMADDLREVVAALKIVGLVERIGDYA
                     KNIAKRVPAIEDSPDLRPLSLLPEMGRIVAEMVRSALDAFVARDSATAASVCERDRAV
                     DDFYNSIFRTLLTFMMESPNNITAATHLLFVAKNLERIGDHATNIAEMVYFAATGGHL
                     GEREKGADTTRPAGQ"
     gene            4126..4824
                     /gene="phoB"
                     /locus_tag="VGB57_10905"
     CDS             4126..4824
                     /gene="phoB"
                     /locus_tag="VGB57_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY0131902.1"
                     /translation="MTKGRILLVEDDRALVELIDFHLAKAGYETTKTASGDEALLLVR
                     ESPPDLVLLDWMIEGTSGIEVCRQLRRMPETKNIPIIMMTARGEETDRIRGLDTGADD
                     YITKPFSPKEMLARVGAVLRRVRPALAGETLTFEDLEMDLATYRVRRGGRPVHLSPSE
                     FRLLRHFMESPRRVFTREGLLDTVWSEGTELDLRTVDVQIRRIRKALNEGGKPDLIRT
                     VRSAGYALESAGGG"
     gene            complement(4942..>7550)
                     /locus_tag="VGB57_10910"
     CDS             complement(4942..>7550)
                     /locus_tag="VGB57_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0131903.1"
                     /translation="EAPAQGSEEAVEVSAPGAEPEEAGDIVVTGRNISNILRNTPAVV
                     SVLSSEDIARTGEGDIAGALQRVTGLSVVGNGFVFVRGLGDRYSLALLNGSPLPSPEP
                     LRRVVPLDIFPTGLIASSVVQKSYSVNYPGEFGGGVINLTTRSIPDETFVTVGGSAEL
                     DGETTGHFGYSYYGSDLDPLGFDDGTRDMPAGLRSAVAGGAFNGATTAARRRDFAAAL
                     VNAPTTLLQRIANVAPNLGGNISAGTAFDLLGARIGILGAVGYSNSWRTRDAIQQISL
                     DPELAGTPQSSFRRVSTDNRVLVNAMLGLGAEIGRHKLRWTNLFIRDTLKQGRLAAGF
                     NRSVQDQDPNLPPSIVEQNSYWFERQLIDTQFVGEFKFGDFSMDLRGTYANSQRESPY
                     ERDISYTWLGDGNPATKSASDVDDYVNNLTSGGQSATIAFNDLGEDVHAGGIDFAYRL
                     NGAIPTTFSAGYAYTKNQRDSSRFQFQYFRPEGALPIAVAQERPDYLLSDFNIYNFNI
                     QLRDISGAEGAAAYEAGLRIHAGYAQAEVELPGPALRAQFGVRYEDAVETVLTSAAGA
                     AATRIANDYWLPAATLTWNFADNMQLRLHASKTIARPQFRELAPQIYQDFESDREFTG
                     NPFLTDSRLTNAEARFEYYFRRGERFSLAGFYKKIDNPIESAAFFAGGGQLRTGFANA
                     PHATLYGVEAELQTYLPLSGLGGGFANKRLLLIANYTWTKSKVKSDDTPVIGPDLQPV
                     AANLLFQDGAPLTGQSDHLANIQFGIERIGRLSQATFLLAYASERVTERGPIQGSARQ
                     PDIVEKPGLRLDFVAREAVRFLGQDAEIKLEARNLLGQDYEEYQEFGDNRIDINSYKV
                     GRTVSVGLSVTF"
BASE COUNT         1175 a         2486 c         2577 g         1312 t
ORIGIN      
        1 gagaaagaag gctcccaatt cctcccccgg gtttccgggg gagggggacc gcgcgaagcg
       61 cggtggaggg gttcttcgga gtcagaaggc ccctccacca tgcttcgcat ggtccccttc
      121 ccctgcaaat gcaggggagg atttctagga tgacccccct cgccctgttc atcgcgatcc
      181 tcggcctcgc cttgctcggc tggctgtcgg cgcgcgctcg cgccatggcc ttcgcgcccc
      241 ccggcgggcc gcggctccat tcgctgcccg gctatcatgg ctggtacgtc gccatctggg
      301 cggcgctgcc ggccctgctg ttcctgctgc tctgggtccc gctgagtgac agcctcgtga
      361 tggactcggt gctcggcagt ccggcggcgg ccaatcttcc gccattcgag atgcagcgcg
      421 aagcgatcct gcaggagacc cgcgagctgg ccgaggggct tcgccagtcc agcttccatc
      481 ccgaagcccg ggcgctcgct ccggtctacg atatggcgct gtcgcgttac cggctgatcg
      541 gaacgatcgc ggccctgctc ctcgccttcg ccggcggcgc cttcgcctac agccggatcg
      601 gacccgcctt ccgggcgcgg acccgggtcg agcggctggt gatgctggcg cttctgctcg
      661 cctcgctgat cgcgatcctg acgacgctcg ggatcgtcct ctcgctgctg ttcgaatcgc
      721 ttcgcttctt cagcatggtc tcgcctatcg agttcctgtt cggctcgacc tggagcccgc
      781 agacggcgat gcgggccgat caggccggct cgtccggggc gttcggggcc attcccctgt
      841 tctggggcac cgtcttcata ggcgccatca tcgcgatgat cgtggcgatc ccgctggggc
      901 tgatgagcgc catctacctc acccaatatg cacgccccgc ggtgcgccgc tggatgaagc
      961 cgctgctcga gatgctcgcc ggcgtcccga ccgtggtcta cggctatttc gcggccctga
     1021 ccgtggctcc cgccgtccgc gatttcggcc ggtcgctcgg aatcccggcc tcttcggaaa
     1081 gcgctctcgc cgccggtctg gtcatgggga tcatgatcat cccgttcgtc tcctcgatgg
     1141 cggacgattc gctgaccgcc gtcccacagt cgatgcgcga cggctcgctc gccatgggcg
     1201 ccacgacgtc ggagacgatc cgcaaggtgc tcgtccccgc cgcgctgccc ggaatcgtcg
     1261 gcggggtact gctcgccgtc agccgggcga tcggcgagac gatgatcgtc gtcatggccg
     1321 cgggcctttc cgccaatctg accgccaacc ccttcgccag cgtcaccacc gtgacgaccc
     1381 agatcgtcca gctgctcacc ggcgaccagg agttcgacag ccccaagacc ctggccgcct
     1441 tcgcgctcgg gctcgtcctg ttcatcgtca cccttctgct caacatcgtc gccctgcgcg
     1501 tggtgaagaa atatcgcgaa gcctatgact gacccgtccc gcctcaagcg ccgctacgcc
     1561 gcggagcgcc gtttccggct tttcgggctg gcggcggtgc ttgtgtccgc cgccttcctc
     1621 gccttcctgc tcggcacaat ggcggttcgc ggcgccggcg gcttcgtcga gacccagatc
     1681 cgggtcgatc ccgccgtgcc gcgggaggtc gccctcgctt cggaactcgc cgccaggaac
     1741 gtcgagcccg gggcaggtcc ggtctggctg cccgcctcgg ccgagatcga cgcggcggcg
     1801 aagggccggg ggagcgacga agctggggcg gcctatgcgc agctgcgcga gcagggccgg
     1861 gcgcggaccc gcttcaactt gaccttcctg acctcggccg atgccaccga tccggcggcc
     1921 gtgggcgtgt ggggcgcctt caagggctcg cacctgacca tgctggtgac tctcgccatc
     1981 gcctttccgg tcggggtcct gtcggccatc tacctcgagg aatatgcgcc gaggaaccgc
     2041 tggaccgacc tcatcgaggt ctcgatcaac aatctcgccg cggtgccgtc gatcatcttc
     2101 ggcctgctcg gcctcgccgt cttcctcaac acgatgcaca tgccgcgctc ggcgccgctc
     2161 gtcggcgggc tgactctggc gctgatgacc atgccggtga tcgtcatcgc cggccgcaac
     2221 gcgatcaagt cggtgccgcc ctcgatccgc gacgccgccc tcggcgtcgg cgcgagccgg
     2281 atccaggtcg tctaccacca cgtcctgccg ctcgccttgc ccggcatcct caccgggacg
     2341 atcatcggca tggcgcgggc gctcggcgag acggcgccgc tgctgatgat cggcatgcgg
     2401 gccttcatct cgaccccgcc gggcgggttc gcggatcccg cgaccgtgct tccggttcag
     2461 atcttcctgt ggtctgacga ggtcagccgc ggcttcgtcg aaaagacctc ggcggcgatc
     2521 atcgtccttc tggtcttcct gatggcgatg aacggcatcg ccatctatct gcggaacaag
     2581 ttcgagaggc gctggtgaaa ccgatggtgg acgaggaaaa cagagcggag gcccacgccg
     2641 ttccggctac cgccgggagc gcgccgaaga tgcacgcgcg gggcgtgaat gtctggtacg
     2701 gcgagaagca ggcgatcaag caggtctcga tcgaagtccg tcaggagcac gtcaccgcct
     2761 tcatcggccc gtccggctgc ggcaagtcga ctttcctgcg caccctcaat cggatgaacg
     2821 acaccatccc cagcgcgcgc atcaccggcg agatcgggct cgacggcgaa gacatctatg
     2881 cgagcggcat ggacgtggtg cagctccgcg cccgggtcgg gatggtgttc cagaagccca
     2941 accccttccc caagtccatc tacgagaatg tcgcctacgg gccgcggatc catgggctgg
     3001 ccgcggggcg gcaggagctc gacgccatcg tcgagcggtc gctgaagcgc gccggcctct
     3061 gggaggaggt gaaggaccgc ctcgccgaga gcggcaccgc gctttccggc ggccagcagc
     3121 agcgcctgtg catcgctcgg gccatcgccg tcgatcccga ggtgatcctg atggacgagc
     3181 cctgctcggc cctggacccc attgccaccg ccaagatcga ggagctgatc cacgagcttc
     3241 gcggccgcta cgccatcgtc atcgtcaccc acaacatgca gcaggccgcg cgggtctccc
     3301 agcggaccgc cttcttccac ctgggcgagc tgctcgaata tggcgagacg tcccagatct
     3361 tcaccaatcc gagggtcgat aggaccaagg attacatcac cggccgctac ggctgaggaa
     3421 cccgcacatg gcaacaggtc acacgctcaa ggctttcgac gacgatctca accagctgcg
     3481 cgccagcatc gccgagatgg gcggcctggc ggaagcggcg atcgccggtt cgatgcgggc
     3541 gctcgccggc cgcgacagcg agggcgcccg ccaggtcgtc gagggcgaca ggaggatcga
     3601 cgctttcgag gcggagatcg agcggctggc ggttcagatc atcgcgttga gggcgccgat
     3661 ggcggacgat ctgcgcgaag tggtcgccgc tctgaagatc gtcgggctgg tcgagcggat
     3721 cggcgattat gccaagaaca tcgccaaaag ggtcccggcc atagaggaca gccccgattt
     3781 gcggccgctc tcgctgctgc ccgaaatggg gcggatcgtc gccgagatgg ttcgaagcgc
     3841 gctggacgcc ttcgtcgccc gggattccgc cacggccgct tcggtgtgcg agcgcgacag
     3901 ggcagtggac gatttctaca actccatctt ccgcaccctg ctcaccttca tgatggagag
     3961 cccgaacaac atcaccgcgg ccacgcacct gctgttcgtc gcgaagaacc tcgagcggat
     4021 cggcgatcat gcgacgaaca tcgccgagat ggtctatttc gcggccaccg gcggccatct
     4081 cggcgagagg gaaaagggcg cggacacgac ccggccggcc gggcagtgac caagggccgg
     4141 atcctgctcg tcgaggacga ccgggcgctg gtcgagctga tcgatttcca cctcgccaag
     4201 gccggatatg agacgacgaa gaccgcgagc ggcgacgagg cgctgttgct ggtgcgcgaa
     4261 tcgccgccgg atctcgttct gctcgattgg atgatcgagg gcacttccgg catcgaagtc
     4321 tgcaggcagc tgcgccggat gccggagacg aagaatatac cgatcataat gatgacggcg
     4381 cggggggagg aaacggaccg gatccgaggc ctggataccg gcgccgacga ttatatcacc
     4441 aagccttttt ccccgaagga gatgctggcg cgggtcggag ccgtgctgag gcgcgttcgg
     4501 ccggcgctgg cgggggagac gctgacgttc gaggatctgg agatggacct cgcgacctat
     4561 cgggtgaggc gcggcgggcg gcccgtccat ctctcgcctt cggaattccg gctcctgcgc
     4621 cacttcatgg aaagcccgcg acgggtattc acgcgcgaag ggctgctcga cacggtgtgg
     4681 agcgaaggca ccgagctcga cctgcgcacg gtcgacgttc agatccgccg gatccgcaag
     4741 gcgctgaacg aaggcggcaa gcccgatctc atccgaaccg tccgctcggc gggctacgcg
     4801 ctggagagcg ccggcggggg gtgagcagga tcgcttcagc ctgacccgcg tccgtcattg
     4861 cgagcgaagc gaagcaatcc agcggctcgg gcgtccgaag ctggattgct tcgtcgcttc
     4921 gctcctcgca atgacggccc ctcaaaaggt gacgctcagc ccgacgctga cggtgcggcc
     4981 gaccttgtag ctgttgatgt cgatcctatt gtccccgaac tcctgatatt cctcgtaatc
     5041 ctggccgagc aggttgcgcg cctcgagctt gatctcagcg tcctggccga ggaagcggac
     5101 tgcctctcga gcgacgaaat cgagccgcag gccgggcttc tctacgatgt cgggctggcg
     5161 cgcgctgccc tgaatggggc cgcgttcggt gacccgctcg ctggcgtagg cgaggaggaa
     5221 ggtcgcctgc gacagccgcc cgattctctc gattccgaac tggatgttgg cgagatggtc
     5281 cgactgcccg gtcagcggcg cgccgtcctg gaacagcagg ttcgcggcca ccggctgcag
     5341 gtccgggccg atgacgggcg tgtcgtccga tttcaccttc gacttcgtcc aggtgtaatt
     5401 ggcgatcagc aggaggcgct tgttggcgaa gccccccccg agcccggaca gcggcaaata
     5461 ggtctggagc tccgcctcga ccccgtagag cgtcgcgtgc ggagcgttgg cgaagccggt
     5521 gcgaagctgg ccgccgccgg cgaagaaggc ggcggattca atggggttgt cgatcttctt
     5581 gtagaagccg gccaggctga accgctcgcc gcgccggaaa taatattcga agcgcgcctc
     5641 ggcgttggtc agccggctgt cggtcaggaa cgggttgccg gtgaactccc ggtcggattc
     5701 gaaatcctgg tagatttgcg gcgccagctc gcggaactgg ggacgggcga tggtctttga
     5761 ggcgtgcagg cgaagctgca tgttgtcggc gaagttccag gtcagggtcg cggcgggaag
     5821 ccagtagtcg ttggcgatcc gggtcgcggc ggcgcccgcg gcgctggtca ggacggtctc
     5881 gaccgcatcc tcgtaacgga ccccgaactg ggccctcagc gccgggcccg gaagctcgac
     5941 ctcggcctgg gcgtagccgg cgtggattct aagccccgct tcgtaggcgg ccgcgccttc
     6001 cgcgcccgaa atgtcgcgga gctggatgtt gaaattgtag atgttgaagt cggagagcag
     6061 atagtccggc cgctcctgcg cgaccgcgat ggggagggcg ccctcgggcc ggaaatactg
     6121 gaactggaag cgcgaggaat cgcgttggtt cttggtgtaa gcatatcccg cggagaaagt
     6181 ggtcggaatc gcgccgttca gccgataggc gaagtcgatg ccgccggcat ggacgtcctc
     6241 gccgaggtcg ttgaaggcga tggtcgccga ctggccgccg gaggtcaggt tgttcacata
     6301 atcgtcgacg tcgctggcgc tcttcgtcgc cgggttgccg tcgcccagcc aggtgtagct
     6361 gatgtcccgc tcgtaggggg attcgcgttg cgagttggcg taggtgccgc gcaggtccat
     6421 gctgaaatcg ccgaacttga actcgccgac gaactgcgtg tcgatcagct gccgctcgaa
     6481 ccagtagctg ttctgctcga cgatcgaggg cggaaggttc gggtcctggt cctggacgct
     6541 tcggttgaag ccggcggcga ggcggccctg cttcagggtg tcgcggatga agaggttggt
     6601 ccacctcagc ttgtggcggc cgatctcggc gccgaggccg agcatggcgt tgaccagtac
     6661 gcgattgtcg gtgctgaccc ggcggaagct cgactgcggc gttccggcga gctccggatc
     6721 gagcgagatc tgctggatcg cgtcgcgcgt gcgccagctg ttgctgtagc cgacggcgcc
     6781 taggattccg atccgcgccc cgagcaggtc gaaagcggtg ccggcgctga tattgccgcc
     6841 gagattgggg gcgacgttgg cgatgcgctg gagcaaggtg gtgggagcgt tgaccagggc
     6901 ggccgcgaag tcgcggcgcc gggcggccgt cgtagcgccg ttgaaggcgc cgccggcgac
     6961 ggcgctcctc agcccggccg gcatgtcgcg ggtgccgtcg tcgaagccga gggggtcgag
     7021 atcgcttccg taataggaat agccgaaatg gccggtggtc tcgccgtcca gctcggccga
     7081 gccgccgacg gtgacgaagg tctcgtccgg gatcgaccgg gtggtgaggt tgatgacccc
     7141 gccgccgaac tcgcccggat aattgaccga atagctcttc tgcacgaccg agcttgcgat
     7201 gagtccggtc gggaagatgt cgagcggaac gacccggcgc aacggctccg ggctcggcaa
     7261 aggagagccg ttgagcagcg ccagcgaata gcggtcgccc aggccccgga cgaagacgaa
     7321 gccgttgccg accaccgaaa gcccggtgac tctctggagc gctccggcga tgtcgccttc
     7381 accggtccgg gcgatgtcct cgctggacag gaccgacacc accgcaggcg tattgcgaag
     7441 gatgttggag atgttgcggc cggtgacgac gatgtcgccg gcctcttcag gctcggcgcc
     7501 gggcgccgag acctcgacgg cctcctccga gccttgggcc ggcgcttcgg
//
LOCUS       DASWNU010000553         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_21716, whole genome shotgun sequence.
ACCESSION   DASWNU010000553 DASWNU010000000
VERSION     DASWNU010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21716"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /gene="tsaB"
                     /locus_tag="VGB57_10915"
     CDS             complement(<1..486)
                     /gene="tsaB"
                     /locus_tag="VGB57_10915"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY0131904.1"
                     /translation="MILIVDTATAACSVALVEGEALVDERHEIVGRGHAERLVPMIEA
                     LLAGRRPDSILVDCGPGSFTGVRVGLAAAHGMAIGWGVPLSGYSSMAAVAAAEGRAGE
                     IAVALEGGHGQLFVQTFGEDVMRPLDTLRSLPPEEAGAAISARRVAGSGARALVAARG
                     SG"
     gene            complement(627..1199)
                     /locus_tag="VGB57_10920"
     CDS             complement(627..1199)
                     /locus_tag="VGB57_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY0131905.1"
                     /translation="MLIQTEATPNPSTLKFLPGRRVMEAGTRDFATPEDSEVSPLAEA
                     IFSTGEVEGVFFGRDFISVTAAPGVEWRDLKPEILSILLDHFSSDAPLFRAASAAAIE
                     VAGDEDYGEDPADAPLVAEIRELIETRVRPAVAQDGGDIVYRGFRNGTVYLALQGACS
                     GCPSSTMTLKQGIESLLKHYVPEVEAVEAV"
     gene            complement(1363..1593)
                     /locus_tag="VGB57_10925"
     CDS             complement(1363..1593)
                     /locus_tag="VGB57_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131906.1"
                     /translation="MPRYYFNVSCEGYESTDVVGECCPNDVAALSGAMRVASAVLQKR
                     LRAEELSDDGWVEVEDERHRPVLRLPLRAAAY"
     gene            complement(1661..2152)
                     /locus_tag="VGB57_10930"
     CDS             complement(1661..2152)
                     /locus_tag="VGB57_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0131907.1"
                     /translation="MDGTDLKILAILQEDASVSVAEIAGRVGLSQTPCWRRIQRLEES
                     GVILRRVALLDPNAIGLGLTVFVEIQTGDHSKAWLDRFAEAVLAMPEVMEVHRMAGDV
                     DYLLRVAVANMNAYDDFYRRLIAGLDIKNVTSRFAMELVKGTTAFPLSAEAASGRGRP
                     QAE"
     gene            2378..3133
                     /locus_tag="VGB57_10935"
     CDS             2378..3133
                     /locus_tag="VGB57_10935"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY0131908.1"
                     /translation="MLRAPGRGMNAFALFSAQPASADPGDRLEGLRAGLDGRIVFTTS
                     FGLEDQAVAHHIFTRKLDIEVSTLDTGRLFPSTYRVWEETERRYGVRIRSWHPDQSAL
                     AAFVAGAGINGFYDSKEARLGCCQVRKVEPLGRALAGAAAWVTGLRADQSAGRGSVEL
                     AAWDPERELVKFAPLYDWSRERVADFCSAEDVPVNALHAKGFPSIGCEPCTRAVAPGE
                     PERAGRWWWEADEARECGLHVGPDGRLVRSKAA"
     gene            3130..>3813
                     /locus_tag="VGB57_10940"
     CDS             3130..>3813
                     /locus_tag="VGB57_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEY0131909.1"
                     /translation="MSRTPAAQLPQREPAATPARIAPLANLPLFHRLAGRKAVVAGSS
                     DGALWKAELLAAAGADVLVLAGEDSEKFEAIPALTVLRRGWTEPDLEGAALAVGDPGS
                     RDEALRFVAAARAAGVPVNVIDQTDLCDVQFGTIVNRSPVVLAISPGGAAPLLGQSIR
                     TRIEAVLPLGLSAWAKAAQALRPRLKARVKSFADRRAFWERFVERAWAGGEPDGTEAL
                     FEPRPRREGR"
BASE COUNT          633 a         1347 c         1241 g          592 t
ORIGIN      
        1 ccccgagccg cgcgccgcga ccagcgcacg ggctcccgag cccgcgaccc ggcgggcgct
       61 tatggccgcc cccgcctcct cgggcggcag ggagcggagc gtgtcgagcg gccgcatcac
      121 gtcctcgcca aaagtctgga cgaaaagctg cccgtgtccg ccctcgagcg caaccgcgat
      181 ctcgcccgcc cgcccctccg ccgccgcgac cgcggccatt gaggaatagc cggacaaagg
      241 tacgccccag ccgatcgcca tcccgtgcgc cgccgccagg ccgacccgga ccccggtgaa
      301 gctgcccggg ccgcaatcga ccagtatcga gtcgggccgc cggcccgcca gaagcgcctc
      361 gatcatcggc accagccgct cggcatggcc gcggccgacg atttcatgac gctcgtcgac
      421 cagagcctcg ccctcgacga gcgcgacgga acaggcggcg gtggcggtgt cgacgatgag
      481 gatcatttgg cgaaaggcac agacgttggt gagagtctcg accgtcaccc tgaacttgtt
      541 tcagggtcca ggcctccacc cgcactgctg ctcgaaactg gatgctgaaa caagttcagc
      601 atgacgatac cgaatgacga cagagttcaa accgcctcga ccgcctcgac ctcgggcaca
      661 taatgtttga gcaggctctc gatcccctgc ttcaaggtca tggtcgagga ggggcagccc
      721 gagcaggcgc cctgcagggc gaggtagacg gtgccgttgc ggaatccccg atagacgatg
      781 tcgccgccgt cctgcgccac cgcgggcctg acccgggtct cgatcagctc gcgtatctcg
      841 gcgacgagcg gggcatcggc cggatcctcg ccataatcct cgtcccccgc cacttcgatc
      901 gcggccgccg aagccgcccg gaacaaaggc gcgtcgctcg agaaatggtc cagcaggatg
      961 ctcaatattt ccggcttaag atcgcgccac tcgacgccgg gagcggcggt gaccgagatg
     1021 aagtcgcggc cgaagaacac gccttccacc tcgccggtgg agaagatcgc ctcggcgagc
     1081 ggcgagactt ccgaatcctc cggagtcgcg aaatcgcgcg tcccggcctc catcacccgc
     1141 cgtccgggca ggaacttcag ggtcgacgga ttgggggtgg cttcggtctg gataagcatg
     1201 gttcggatgt gggccgatgg gagcggccga tcaagtagga gccagtcccg tcattgcgag
     1261 gagagcagcg acgaagcaat ccagaggtcc cgaagtccgc actggattgc ttcgcttcgc
     1321 tcgcaatggc ggaccaggaa ggcaataccc taataagcaa ccctaataag ccgccgccct
     1381 cagcggaagc cggagcaccg gccgatgccg ttcgtcctcc acctcgaccc agccatcgtc
     1441 ggaaagctcc tcggcgcgaa gcctcttctg gagcaccgcg cttgccaccc tcatcgcgcc
     1501 gctcagggcg gcgacgtcgt tcgggcagca ttcgccgacg acgtcggtcg actcataccc
     1561 ctcgcaggag acattgaaat aatagcgtgg cacgcgcctc gaacgctcca aaccggcccg
     1621 acgcctcatt ccgcactgca ataaagctgt caatcgacct tcactccgcc tgagggcggc
     1681 cccgtccgga agccgcttcg gccgaaagcg gaaaggcggt ggtgcccttg accagctcca
     1741 tcgcgaatcg cgaggtgacg ttctttatgt ccaggccggc gatcagccgc cggtagaagt
     1801 cgtcataggc gttcatgttg gcgaccgcga cccggaggag ataatcgacg tcgccggcca
     1861 tccggtgcac ctccatcacc tccggcatgg cgagcacggc ctcggcgaag cggtcgagcc
     1921 aggccttcga atgatcgccg gtctggatct cgacgaagac ggtgaggccg aggccgatcg
     1981 cgttcggatc gagcaaggcg acccgccgga ggatcacccc gctttcctcg agtcgctgga
     2041 ttcggcgcca gcagggcgtc tgggagaggc cgacccggcc ggcgatctcg gcgaccgaca
     2101 cggaggcgtc ctcctgaagg atggccaata tcttaaggtc ggtgccgtcc atctaatccc
     2161 tgcccggatt tgagtatatt attctaataa ctcgtacggc ccggcaataa tagcgccttt
     2221 tgcgccgagc cattgaagac gtgctttttg gcggataact tgggttttca atggccggac
     2281 agggcaaatt cgcgcctgaa aggaagttgc ggcgcctcca ccgcaattag aaggaaattc
     2341 taccagtgac ccggttacga gcctttatgt cccgccggtg ctccgagcgc cgggccgcgg
     2401 catgaacgcc ttcgccttgt tttccgcgca gccggcttcg gcggatccgg gcgaccggct
     2461 cgaagggctt cgggccgggc tcgacggtcg aatcgtcttc accaccagct tcggcctcga
     2521 ggatcaggcc gtcgcccacc acatcttcac ccgcaagctc gacatcgagg tgtcgaccct
     2581 cgacaccggc cgccttttcc cctcgaccta cagggtctgg gaggagaccg agcggcgcta
     2641 cggagtccgg atccgctcct ggcatccgga ccagtcggcg ctggccgcct tcgtcgccgg
     2701 cgccgggatc aacggcttct acgactcgaa ggaagcgcgg ctcggatgct gtcaggttcg
     2761 caaggtcgag ccgctcggcc gcgccctggc cggcgccgcc gcctgggtga cggggctgag
     2821 ggcggaccag tcggccggcc ggggcagcgt cgagctcgcc gcctgggatc ctgagcgcga
     2881 gctggtcaag ttcgcgccct tgtacgactg gagccgcgag cgggtcgccg atttctgctc
     2941 ggccgaggac gtcccggtca acgcgctcca tgccaagggc ttcccgtcga tcggctgcga
     3001 gccctgcacc cgagccgtcg ctccgggcga gccggagcgg gccggccgct ggtggtggga
     3061 ggccgacgag gccagggaat gcggcctcca tgtcggcccc gacggccggc tggtgaggag
     3121 caaggcggca tgagccggac ccctgcagcg cagctcccgc agcgcgagcc ggccgcgact
     3181 cccgcccgga tcgctccgct cgccaatctt ccactcttcc accgcctggc cggccgcaag
     3241 gcggtcgtcg ccggaagctc ggacggcgcc ctgtggaagg cggagctgct cgccgccgcc
     3301 ggagccgacg tcctggtcct cgccggcgag gattcggaga agttcgaagc catccccgcg
     3361 cttaccgtgc ttcggcgggg ctggaccgag cccgacctcg agggcgccgc cctcgccgtc
     3421 ggcgatcccg gcagccggga cgaggccctg cgcttcgtcg cggcggcgcg ggccgccgga
     3481 gtcccggtca acgtcatcga ccagaccgat ctgtgcgacg tccagttcgg gaccatcgtc
     3541 aaccgctcgc cggtcgtcct cgccatctcc cccggcggcg ccgcacccct gctcggccag
     3601 tcgatccgaa cccggatcga ggccgtcctt ccgctcggcc tttccgcctg ggcgaaggcg
     3661 gcgcaggcct tgcgtccccg cctcaaggca cgggtgaaga gctttgccga ccgccgcgcc
     3721 ttttgggagc gtttcgtcga gcgtgcctgg gccggcggcg aaccggacgg caccgaggcc
     3781 ctgttcgagc cgcgcccccg ccgagagggc cgc
//
LOCUS       DASWNU010000554         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_152250, whole genome shotgun sequence.
ACCESSION   DASWNU010000554 DASWNU010000000
VERSION     DASWNU010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152250"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="VGB57_10945"
     CDS             complement(<1..1789)
                     /locus_tag="VGB57_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131910.1"
                     /translation="MTAAPALEEAASSRERFAKPLFLFTVLTGSFLLFLTQPMIARMA
                     LPRLGGAPAVWNSAMLVYQGLLLAGYAYAHFLTRLRPRRQAGLHLALFAVAALWLPIG
                     VSDAVLPADAVPALWAPWFLASSIGPLFFIVSAQAPLMQRWYALEGARGDPYPLYAAS
                     NLGSFAGLLSYPLLVEPLLTLEQQSLVWSAGYLLLVMMVVACALTVPAAAVEAMPEAD
                     GPAPSWRRLLHWVALAAVPSGLMLSTTTHLTTDIVAVPLLWVLPLALYLLSFVIAFAH
                     RRGAANLVVAIAPLFLLVAGGFAFSGGSDHPFFTATLGLLLLFIIAVALHAEMYRRRP
                     EPAHLTAFYLAMAVGGVLGGVFCAILAPTLFDWAYEHPLLIVAAAVLVPQTPFIPWAQ
                     GIPAPLRWLIAAGLLALSWTVDGNIGPFDGTGSALVGSIFISLVTLAFIGRRWLFAAG
                     LLALMLSYGGWTILQQSTTDIRTRSYFGIYQVTTFVDGNGRPIEMRLTHGTTLHGVQN
                     MLPGTETVATSYYARRSGVGEVMANAPILFGPAARVGVVGLGSGTLSCYKQPGQSWSI
                     FEIDPAMVEIARTRFSFLSRCAPDARIILG"
     gene            complement(1786..2781)
                     /gene="hemB"
                     /locus_tag="VGB57_10950"
     CDS             complement(1786..2781)
                     /gene="hemB"
                     /locus_tag="VGB57_10950"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0131911.1"
                     /translation="MSHAAFPALRLRRTRAAAWSRALFAETILTPADLIWPLFISGGE
                     GAEEPIASLPGVSRRSVDRLGAMAREARDLGIPCIALFPNTPRELRTGDAREALNPDN
                     LICRAVRAVKEAAPEIGVLTDVALDPYTAHGHDGLVDAAGYVVNDSTVAMLVEQALVQ
                     AAAGADIVAPSDMMDGRVGAIRQALEREGHVNVQIMAYAAKYASAFYGPFRDAVGSSG
                     LLKGDKKCYQMDPANSEEALREVALDLAEGADSVMVKPGLPYLDIVRRVKERFEVPVF
                     AYQVSGEYAMIEAAAAAGAGDRDALVLETLMAFKRAGCSGVLTYHAPVAARLLAR"
     gene            2930..3373
                     /locus_tag="VGB57_10955"
     CDS             2930..3373
                     /locus_tag="VGB57_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131912.1"
                     /translation="MKFTKKFALRAGIVAAATVAVTGPAIAQPNPVLYLTGLEYYSAG
                     GKNWVRHRYDVMNKDEYPAAMFAPAPALPPCGTNTNSSRSWVDFFDSRGKRLYGFCAL
                     GDPANLGKIWFATEEGTIPPSYVYIEIHDRQANKKYKSNLADTTP"
     gene            3505..4398
                     /locus_tag="VGB57_10960"
     CDS             3505..4398
                     /locus_tag="VGB57_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0131913.1"
                     /translation="MPLNRPVPKEAVLVVNAASRKGQALFREASDKLRDSGIVLTGAF
                     ALRDPRLLVPTVKEAVRSGAPMVIVGGGDGSLSYTVDEVVDRDCVFALLPLGTANSFA
                     RTLGIPLDLDGAIKVIATGQRRRIDLGMIDDDYFANCAAMGLSPMIGEGVPHNLKRYL
                     GRVGYLIWAVWCLIRFHAFKLTVRGEGVDETFWATEVRIANGGHHGGVELIESAEVDS
                     GQIIVQAVVGRRASSLVWDWFAKYFKLPTRNRTTREFHAARLTIETRPRQRISIDGEV
                     LAKTPVVAQVAERAIEVVVPA"
     gene            complement(4408..5871)
                     /locus_tag="VGB57_10965"
     CDS             complement(4408..5871)
                     /locus_tag="VGB57_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0131914.1"
                     /translation="MAKIEAQSRPRQRDLTSGAIGPTLLAFALPTLGSSILQSLNGSI
                     NAVWVGRFLGEDALAATSNANIVMFLMTSFVFGFGMASTILIGQSFGRGDVDGARRVI
                     GTATGAFVAASLLVSGAGWLFAPDILRMLGTPADAAPLALAYLRVIFIAMPPALIMVS
                     WMMALRGSGDAMTPLWFMILAVILDSGLNPVFILGLGPAPRLGIAGAATATVIANFVS
                     LAGLLVYVYARDLPLRLRGAELRYLKPGPGLLATIVRKGLPMGLQMIVISASALALIG
                     LVNREGVHTAAAFGVAMQLWTYLQMPAMALGAAVSAMVAQNIGAGRWDRVSAITRSGV
                     TFALLITLGLVILLALADRPALALFLGGDSPALPIARHIQILATWSFLLFGVTLVLFG
                     TVRANGSVIGPLIILFVGLVPIRLGFALGAYPWLGADALWLSFPVSSFANMAMAIAFY
                     LHGGWRKSRMGPPPDPEECLEEAQATAEPGGRLNPTG"
BASE COUNT         1098 a         1993 c         2105 g          828 t
ORIGIN      
        1 cacccaggat gatccgcgcg tccggcgcgc acctgctgag gaaggagaag cgggtgcgcg
       61 caatctccac catcgccggg tcgatctcga agatgctcca gctctggccg ggctgcttgt
      121 agcagctcag cgtgccgctc cccaggccca ccacgccgac gcgcgccgcc ggcccgaaca
      181 atatcggcgc attggccatc acctctccga ccccggagcg gcgcgcataa tagctggtgg
      241 ccacggtctc cgtccccggc agcatgttct gcacgccgtg cagcgtcgtc ccgtgcgtca
      301 gccgcatctc aatcggccgg ccattgccgt ccacgaacgt ggtgacctga tagatgccga
      361 aataggagcg ggtgcggatg tcggtggtgg attgctggag gatggtccag ccgccgtagc
      421 tcagcatcag ggcgagcagg ccggccgcga agagccagcg ccggccgatg aaagcgaggg
      481 tgacgagcga gatgaatatg ctcccgacga gagcgctgcc tgtcccgtcg aaaggtccga
      541 tattgccgtc gaccgtccag gacaaagcga gcaggccggc ggcgatgagc cagcgcagcg
      601 gcgccggaat gccctgcgcc caggggatga acggagtctg cggcacgagc acggccgcgg
      661 cgacgatcag cagcggatgt tcataggccc agtcgaacag ggtcggcgcc aggatggcgc
      721 agaacacccc gccgagaacg ccgccgaccg ccatggcgag gtagaaagcg gtgaggtgcg
      781 ccggttcggg ccgccgccgg tacatctcgg cgtggagggc gacggcgatg atgaagagca
      841 gcagcagacc cagggtcgcg gtgaagaagg gatggtcgga tccgccgctg aaggcgaagc
      901 cgccggcgac gaggaggaac agaggcgcga tcgcgacgac caggttggcc gccccgcgcc
      961 ggtgggcgaa ggcgatgacg aagctcagca gatagagggc gagcggaagc acccagagca
     1021 aggggacggc gacgatgtcg gtggtgaggt gggtcgtcgt cgacagcatc agcccggaag
     1081 gcaccgcggc gagcgccacc cagtggagca gccggcgcca cgaaggcgcc ggcccgtcgg
     1141 cttccggcat cgcctcgacc gccgcggccg gcacggtgag ggcgcaggcc accaccatca
     1201 tcacgagcag aagatagccg gccgaccaga ccaggctctg ctgctccagg gtcagcaaag
     1261 gctcgaccag cagggggtag gagagcaggc ccgcgaagct gccgagattg gaggcggcgt
     1321 agagcggata gggatcgccg cgagcgccct ccaaggcgta ccagcgctgc atcagcggcg
     1381 cctgggcgga gacgatgaag aagagcggcc cgatcgagct cgccaggaac cagggcgccc
     1441 acaaggccgg cacggcgtcg gccgggagca ccgcgtcgga gactccgatg ggcagccata
     1501 aagcggcgac ggcgaacaag gcgagatgaa gccccgcctg gcgccgaggt cgaagccggg
     1561 tcaggaaatg ggcgtaagcg tagccggcga ggagcaggcc ctgatagacc agcatcgcgc
     1621 tgttccacac cgccggcgcc ccgcccagcc gaggcagggc catccgggcg atcatcggct
     1681 gggtgaggaa gagcaggaag gagccggtga gcacggtgaa caggaaaagc ggcttggcga
     1741 accgctcccg cgaggaggcc gcctcctcga gcgccggggc cgccgtcacc gggcgaggag
     1801 ccttgcagcc accggcgcat ggtaggtgag tacgcccgag cagccggccc gcttgaacgc
     1861 catcaatgtc tccagaacca gcgcatcgcg atcaccggca ccggccgcgg cggcggcctc
     1921 gatcatcgca tattcgcccg agacctggta ggcgaagacc ggcacctcga agcgctcctt
     1981 cacccgccgg acgatgtcca gataaggcag gccaggcttg accatcacgc tgtcggcgcc
     2041 ctcggcgagg tcgagcgcga cctcgcgcag cgcctcctcg gaattggccg ggtccatctg
     2101 atagcatttc ttgtcgccct tgagcaggcc gctcgagccg acggcgtcgc ggaaggggcc
     2161 gtagaaggcg ctcgcatatt tggcggcata ggccatgatc tggacgttga catggccctc
     2221 gcgctcgagc gcctggcgga tggcgccgac ccggccgtcc atcatgtcgg agggagcgac
     2281 gatatccgcc cccgccgccg cctgcaccag cgcctgctcg acgagcatcg cgacggtgga
     2341 atcgttgacc acgtaaccgg ccgcgtcgac cagcccgtca tggccgtgcg cggtataggg
     2401 atcgagcgcg acgtcggtga gcactccgat ttcgggcgcc gcctccttca ccgcccggac
     2461 cgctcggcag atgaggttgt cggggttgag cgcctcgcgc gcatccccgg tccgaagctc
     2521 gcgcggcgta ttggggaaga gcgcgatgca ggggatgccg agatcgcgcg cttcgcgcgc
     2581 catcgcaccc agcctgtcga ccgaccggcg cgagacgccc ggcaggctgg cgatcggctc
     2641 ttccgccccc tccccgccgg agatgaacag gggccatatc aggtccgccg gagtgagtat
     2701 cgtctccgcg aacagcgccc ggctccaggc ggcggcgcgg gtgcggcgga gccggagggc
     2761 tgggaaagcg gcatggctca tggagcgagg ctgtaacgcc ccttcgagcc cctgcaaagc
     2821 gcggctcgac gacaggcggg acttccttgt gccgcacccg gaacgatgtc attcttcaga
     2881 cgcgaataca aacttccgcg ctcactctct ggggatgaaa gggcagtcga tgaagttcac
     2941 caaaaagttc gccttgcgcg cagggatcgt cgccgcggcg acggtcgccg tgacggggcc
     3001 ggccatagcc caacccaatc cggtcctcta cctcaccggc ctcgaatatt attccgccgg
     3061 cgggaagaac tgggtccggc accgctacga cgtgatgaac aaggacgagt atccggccgc
     3121 catgttcgcg cccgcgcccg cgctgccgcc gtgcgggacc aacaccaatt cgtcccgatc
     3181 ctgggtcgat ttcttcgatt cgcgcggcaa gcggctctac ggcttctgcg cgctgggcga
     3241 cccggccaat ctcggtaaga tctggttcgc gacggaggag ggcacgatcc cgccgagcta
     3301 cgtctacatc gagatccacg accgccaggc caacaagaag tacaagtcga acctggcgga
     3361 cacgacgccc tagcgtctta atcctcccca ggttggagtc agcaaggccc ctccaccagc
     3421 cttcggctgg tccccctccc ccggaaatcc tggggaggaa ttaggtcttc ggcacttgcg
     3481 ccccatcgcg ggcgttgcca ccttgtgccc ttgaaccgtc ccgttcccaa agaagccgtc
     3541 ctcgtcgtca acgccgcctc ccgcaagggg caggcgctgt tccgcgaggc ttcggacaag
     3601 ctccgcgact cggggatcgt gttgaccggc gccttcgctc tgcgcgaccc gcgcctgctc
     3661 gttccgacgg tcaaggaagc tgtgagaagc ggagcgccga tggtgatcgt cggcggcggc
     3721 gacggctcgc tctcctacac ggtcgacgaa gtggtggacc gcgattgcgt cttcgcgctg
     3781 cttccgctcg gcaccgccaa cagcttcgcc cggacgcttg gaattcctct cgacctcgac
     3841 ggagcgatca aggtgatcgc gacggggcag cggcggcgga tcgatcttgg aatgatcgac
     3901 gacgattatt tcgccaattg cgcggcgatg ggcctgtcgc cgatgatcgg cgagggcgtg
     3961 ccgcataacc tcaaacgcta tctcggccgg gtcggctatc tgatctgggc ggtctggtgc
     4021 ctgatccggt tccacgcctt caagctcacc gtccggggcg aaggcgtgga cgagactttc
     4081 tgggcaaccg aggtccggat cgccaatggc gggcatcatg gcggagtgga gctgatcgag
     4141 agcgccgagg tcgacagcgg ccagatcatc gtccaggcgg tggtcgggcg cagggcctcc
     4201 agcctggtct gggactggtt cgccaaatat ttcaagctcc cgacccgcaa ccggacgacc
     4261 cgcgagttcc acgccgccag gctcaccatc gagacccggc cgcgccagcg aatctcgatc
     4321 gacggggagg tgctggccaa gaccccggtc gtcgcccagg tcgcggagcg ggcgatcgag
     4381 gtggtggtgc cggcctgatc cgtcgcctta ccccgtcgga ttgagccttc cgccaggctc
     4441 ggccgtcgcc tgggcttcct cgagacattc ctccgggtcg ggcggcgggc ccatccggct
     4501 cttccgccag ccgccgtgga ggtagaaggc gatagccatc gccatgttgg cgaaggagct
     4561 gaccgggaag ctcagccaca aggcgtcggc gccgagccag ggataggcgc cgagcgcgaa
     4621 gccgaggcgg atcggaacca ggccgacgaa caggatgatc agcgggccga tcaccgagcc
     4681 gttcgcccga accgttccga acaggacgag ggttactccg aacaggagga agctccaggt
     4741 ggcgaggatc tggatgtggc gggcgatcgg aagggccggg ctgtcgccgc cgaggaacag
     4801 ggccagcgct ggccggtcgg cgagcgcgag caggatgacc agcccgaggg tgatcagaag
     4861 cgcgaaggtc acgcccgagc gggtgatcgc gctcacccgg tcccagcggc cggcgccgat
     4921 attctgggcg accatcgcgc tcaccgccgc gcccagcgcc atcgccggca tctgcagata
     4981 ggtccaaagc tgcatcgcca ccccgaaggc ggcggcggtg tgcacgccct cgcggttgac
     5041 gaggccgatc agggccagcg ccgaagcgga gatgacgatc atctgcagcc ccatcggaag
     5101 ccccttgcgg acgatggtcg caagcaaacc cgggcccggc ttcagatagc gcagctcggc
     5161 gccgcgcagc cgcagcggca aatcgcgcgc atagacatag acgagcaggc ccgcgaggct
     5221 gacgaaattg gcgatcaccg tcgccgtcgc cgccccggct atcccgagcc ggggcgccgg
     5281 ccccaggccc aggatgaaga cggggttgag cccgctgtcg aggatcaccg ccaggatcat
     5341 gaaccagagc ggagtcatgg cgtcgccgct gccgcgaagc gccatcatcc aggagaccat
     5401 gatgagggcc ggcggcatgg cgatgaagat cacccgcaga taggcgagcg cgagcggggc
     5461 ggcgtcggcg ggtgtcccga gcattctcaa tatatcggga gcgaacagcc accccgcacc
     5521 cgagacgagg agcgaggcgg cgacgaacgc gccggtggcc gtgccgatga cgcgccgcgc
     5581 cccgtccacg tcgccgcggc cgaagctctg gccaatgaga atggtgctgg ccatgccgaa
     5641 gccgaagacg aaggacgtca tcaggaacat gacgatattg gcgttggacg tcgccgcgag
     5701 cgcgtcctcc ccgaggaagc ggcccaccca gacggcgttg atcgagccgt tcagcgactg
     5761 caggatcgag gaaccgagcg tcgggagggc gaaggcgagc agggtcgggc cgatggcgcc
     5821 ggaggtgagg tcgcgctggc gggggcgaga ctgggcttca atcttggcca tggctccctt
     5881 tctccgtcat cccggcgaaa gccgggacct caggtcgata agtcccggcg ggtctccccg
     5941 aggtcccggc tttcgccggg atgacgtcga cccgcgctta ccgagcccat tcgcggtcgc
     6001 ggtcgagcga aagcgaattc cgac
//
LOCUS       DASWNU010000555         5144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_332094, whole genome shotgun sequence.
ACCESSION   DASWNU010000555 DASWNU010000000
VERSION     DASWNU010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332094"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VGB57_10970"
     CDS             complement(<1..378)
                     /locus_tag="VGB57_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131915.1"
                     /translation="MPSHADAVASLYRPMEHGGWIVRRWGNAIPLRGYWSPARPVSGV
                     ISLDRGSDTWMSLMPVEVESQQIGVDCAAGHVAVFGLGLGWTTALCALKDEVEAVTVV
                     ERDSQLIALHGALGLLDGLPGGAG"
     gene            complement(407..1561)
                     /locus_tag="VGB57_10975"
     CDS             complement(407..1561)
                     /locus_tag="VGB57_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY0131916.1"
                     /translation="MAGIGLDFALGEMADAIRDTTERFAADRIAPLAARIDSEDWFPR
                     ELWPEMGALGLHGITVEEEYGGLGLGYLEHVVAQEEVARASASIGLSYGAHSNLCINQ
                     IRRWASPEQKAKYLPGLISGEHVGSLAMSEVGAGSDVVSMKLRAEKVQGGYVLNGTKF
                     WITNAHYADTLVVYARTGEGSRGITTFLIEKDMPGFSLGQKLDKMGMRGSPTGELVFN
                     DCEVPDENVMGPVNGGVGVLMSGLDYERTVLAGIQLGIMQACLDVVLPYVRERKQFGT
                     PIGSFQLIQAKVADMYVALNSARAYVYTVAKNCDAGKTTRFDAAGAILLASESAVKVS
                     LEAIQALGGAGYTKDWPVERFMRDAKLLDIGAGTNEIRRMLIGRELIGAG"
     gene            1750..2940
                     /locus_tag="VGB57_10980"
     CDS             1750..2940
                     /locus_tag="VGB57_10980"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0131917.1"
                     /translation="MTAQDPIVILSYARTPMGSFQGALAGASATELGAAAVRAAVERA
                     GISGEEVDKIFMGCVLPAGLGQAPARQAALGAGLPRSVEATTVNKVCGSGMQAAMFAH
                     DMLVAGSADLVVAGGMESMSNSPYLMKKHRSGARIGHDTVYDHMMLDGLEDAYDAGRA
                     MGTFAEDSAREYQFTRGQQDEYAIESLRRATEAIRSGAFDRETTPVTVKTRKGEDVVV
                     CDEQPGKSNPEKIPGLKPAFAKDGTITAANASSISDGAAALVITRQSVADRFGMKPVA
                     RIVAHAAHAQAPNLFTTAPVPAVRKALEKAGWGVGDVDLWEVNEAFACVAMIAMRDLG
                     IPREQLNVHGGATALGHPIGASGARLLATLLSALETHGQKRGLATLCIGGGEATAMAV
                     ELVG"
     gene            complement(2962..3492)
                     /locus_tag="VGB57_10985"
     CDS             complement(2962..3492)
                     /locus_tag="VGB57_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131918.1"
                     /translation="MKIVDLVRSSSALCLMTVPATVAIAQSASDPAPVIEAFNETCRR
                     GFPNLETVRLRAESQGWIRRSARMIAEGTDPRLRNVAMPEIYGKGDMMLVLSTPNALS
                     GKSSCGISASGKKTMDTATLAAAVSAALDGAQASIGKERGVEAATWHLQSGLVVRASV
                     SKPGRIRTVNLTVLTS"
     gene            complement(3586..4707)
                     /locus_tag="VGB57_10990"
     CDS             complement(3586..4707)
                     /locus_tag="VGB57_10990"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0131919.1"
                     /translation="MQISDLETPALVVDPARLRHNIERMRRRLAGLGVPLRPHVKTAK
                     SIDVVRLAVEGQPGGITVSTLREADYFLDHGFTDILYAVGIVPSKLPNVATLQQRGAD
                     VTIIVDSLEMAEAVAREADVLGTVFPVLIEVDTDGGRAGVPPEDPRLVEIGRILDSAR
                     GTQLRGVMTHAGLSYGCRSPEALRAHAERERSGAVAAAEALRSAGLPAPVVSVGSTPT
                     ALFAERLDGVSEVRAGVYMFMDLVMADLGVCAQEEIALSVLSTIIGHRRDLGYLLVDA
                     GGLALSKDSGPALEKPSYGSLWDAATARPLGLQIASTNQEHGLVPAGEEDFARLPIGS
                     QVRIAPNHACMTAAAHGRYNVVEGGEAEVLAVWERCNGW"
     gene            complement(4770..>5144)
                     /locus_tag="VGB57_10995"
     CDS             complement(4770..>5144)
                     /locus_tag="VGB57_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131920.1"
                     /translation="SRERAGTIDGVNLFFGALLGANLGTLNSLTLRQYTSFILFLAGT
                     VMALRMISTSERRGFAVGFVAAYAVLIVAMWNIPALQPRGLPEADLHRTLATLAIWIG
                     TIVLQEFAPVLEDPAEAARPQA"
BASE COUNT          826 a         1875 c         1657 g          786 t
ORIGIN      
        1 gccggcgccg ccgggcagcc cgtcgagcag gccgagcgcg ccgtgcagcg cgatcagctg
       61 ggagtcgcgt tccaccaccg tcaccgcctc cacctcgtcc ttcagcgcgc acagggccgt
      121 cgtccacccg aggccgaggc cgaacaccgc gacgtggccg gcggcgcaat cgactccgat
      181 ttgctggctt tcgacctcca ccggcatcag cgacatccag gtgtcgctgc cccggtcgag
      241 cgagatcacg cccgacacgg gacgcgcggg gctccaatag ccgcgaagcg gaatggcgtt
      301 cccccagcgg cggacgatcc agccgccatg ctccatcggc cggtacaggg acgcgaccgc
      361 gtcggcgtgt gaaggcatgt gcgagccgcc cttcgcctgc cccgcctcag cccgccccga
      421 tcagctcgcg gccgatcagc atccgccgga tctcgttcgt gcccgcgccg atgtcgagca
      481 gcttggcgtc gcgcatgaag cgctcgaccg gccagtcctt ggtgtagccg gccccgccca
      541 gggcctggat cgcctccagg ctgaccttca ccgcgctttc ggacgcgagc aggatggcgc
      601 ccgcggcgtc gaaccgggtc gtcttgcccg catcgcaatt cttggcgacg gtgtagacat
      661 aggccctcgc cgaattgaga gcgacataca tgtccgccac cttcgcctga atcagctgga
      721 agcttccgat cggggttccg aactgcttcc tctcgcggac gtaggggagg acgacgtcga
      781 ggcaggcctg catgatcccg agctggattc cggcgaggac ggtgcgctcg taatcgaggc
      841 cgctcatcag aacgccgacg ccgccgttga ccggccccat cacattctcg tccggcacct
      901 cgcaatcgtt gaagacgagc tcgcccgtgg gcgagccgcg catccccatc ttgtcgagct
      961 tctgccccag gctgaagccc ggcatgtcct tctctatgag gaaggtggtg atcccgcggc
     1021 tcccctcccc cgtcctggcg tagacgacga gcgtgtcggc ataatgggcg ttggtgatcc
     1081 agaacttcgt gccattgagg acatagccgc cctgcacttt ctcggccctg agcttcatcg
     1141 acaccacatc cgagcccgcc ccaacctcgg acatggcgag cgagccgaca tgctcgcccg
     1201 agatcaatcc gggcaggtat ttcgccttct gctccgggct cgcccagcgc cggatctggt
     1261 tgatgcacag gttcgaatgc gcgccgtagg aaagcccgat cgaggccgag gcccgggcca
     1321 cttcctcctg ggccacgaca tgctcgagat agccgaggcc gagcccgcca tattcctcct
     1381 cgacggtgat gccgtgaagg ccgagcgccc ccatttccgg ccacagctcc cttgggaacc
     1441 agtcctcgga gtcgatcctg gcggcgagcg gcgcgatcct gtcggcggcg aagcgctccg
     1501 tggtgtcgcg gatcgcgtcc gccatctcgc cgagcgcgaa gtcgagcccg attccggcca
     1561 tgccatttct cctgttcggg ggcggcccta gcagagcctt cgaagtttga acaagcgggg
     1621 cgtcttccct atgtgggccg cgtcgctaac ccgtcgcacc agcgaaggct ggggccccgg
     1681 cgggtgaaga ccaaagacgc ctttcacccc tgggacccca gcctgcgctg gggtgacgaa
     1741 ggaagcaaca tgaccgccca agaccccatc gtcatcctct cctacgcccg aaccccgatg
     1801 ggcagcttcc agggcgccct cgccggcgcc agcgccaccg agcttggcgc cgccgccgtc
     1861 cgcgccgcgg tcgagcgggc cggaatctcg ggagaggaag tcgacaagat cttcatgggc
     1921 tgcgtcctcc ccgccggcct cggccaggcc ccggcccgcc aggcggcgct cggcgccggc
     1981 cttccccgct cggtcgaagc caccaccgtc aacaaggtct gcggctccgg aatgcaggcg
     2041 gcgatgttcg cccacgacat gctggttgcc ggctccgccg acctcgtcgt cgcgggcggc
     2101 atggagtcca tgtccaactc gccctacctt atgaagaaac accgctccgg cgcccgcatc
     2161 ggccacgata cggtctacga ccatatgatg ctcgacggcc tcgaggacgc ctacgatgca
     2221 ggccgcgcga tgggcacctt cgccgaggat tcggcgcgcg aatatcagtt cacccgcggg
     2281 caacaggacg aatatgcgat cgagagcctg cgccgggcca ccgaggcgat caggagcggc
     2341 gccttcgacc gcgagaccac gcccgtcacg gtcaagaccc gcaagggcga ggacgtcgtc
     2401 gtctgcgacg agcagcccgg caagtcgaac ccggagaaga tccccggcct caagcccgct
     2461 ttcgccaagg acggcaccat caccgccgcc aacgcctcgt cgatttccga cggcgccgcg
     2521 gccctcgtca tcacccgcca gagcgtcgcc gaccgcttcg ggatgaagcc cgtcgcccga
     2581 atcgtcgccc acgccgccca cgcccaggcg cccaacctct tcaccaccgc ccccgtcccg
     2641 gccgtccgca aggcgctcga gaaggccggc tggggagtcg gcgacgtcga cctgtgggag
     2701 gtcaacgagg ccttcgcctg cgtcgcgatg atcgccatgc gcgacctcgg cattccccgc
     2761 gagcagctca acgtccacgg cggcgccacc gcgctcggcc acccgatcgg agcgagcggc
     2821 gcccggctgc tggccacctt gctcagcgcg ctcgagaccc acggccagaa acgcggcctc
     2881 gcgacgctct gcatcggcgg cggcgaagcg acggcgatgg cggtggagct ggtcggatag
     2941 ccgctcccgg gcggagacgg ctcagctggt gaggacggta agattgacgg tgcggatccg
     3001 gcccggcttg ctgacgctcg ctctcacgac caggccggat tggagatgcc aggtcgccgc
     3061 ctccacgccc cgctccttcc cgatgctcgc ttgcgcaccg tccagcgccg ccgagacggc
     3121 cgccgcgagc gtggccgtgt ccattgtctt tttgcccgag gccgagatgc cgcagctgga
     3181 cttgcccgag agcgcattag gggtggagag gaccagcatc atgtcgcctt tcccgtagat
     3241 ttccggcatg gcgacgttgc ggagcctggg atcggttccc tcggcgatca tccgagcgga
     3301 gcgccggatc catccctgcg attccgcccg caggcgtacc gtctccaggt tcgggaagcc
     3361 tcggcggcaa gtctcgttga aggcttctat gacaggggcc ggatcggacg cgctctgggc
     3421 gatggcgacg gtcgcgggga cggtcatcag gcaaagcgcg ctgctgcttc gaactaaatc
     3481 gacgatcttc atgggctgtc ctccgagccc cgcctggata agccgccggg ccttaaggca
     3541 aagctaatct tcaacgtgtc ggcgcctccc ggcgcggcgc cccccctacc agccgttgca
     3601 ccgctcccac accgcaagca cctccgcctc gccgccctcc acgacattat agcgcccgtg
     3661 cgccgcggcg gtcatgcagg cgtggttggg ggcgatccgg acttggcttc cgatcggcag
     3721 gcgggcgaaa tcctcctcgc ccgccggcac cagcccatgc tcctggttgg tcgaggcgat
     3781 ttggagcccg agcggacgag cagtcgccgc gtcccagagg ctgccgtagc tcggcttctc
     3841 cagcgccggg cccgaatcct tggacagtgc gaggccgccg gcgtcgacca gcagatagcc
     3901 gaggtcgcgg cggtggccga tgatcgtgga gaggaccgaa agggcgatct cctcctgcgc
     3961 gcagaccccg agatccgcca tcaccaggtc catgaacatg tagacccccg cccgtacctc
     4021 gctgactccg tcgagccgct cggcgaacag ggcggtggga gtcgagccga cgctgaccac
     4081 cggcgccggc aggccggccg agcggagcgc ttcggccgcg gccacggccc ccgagcgctc
     4141 ccgctcggca tgggccctga gcgcctcggg cgagcggcag ccgtaggaaa ggccggcatg
     4201 ggtcatcacc ccgcgcagct gcgtcccccg tgccgaatcg aggatccggc cgatctcgac
     4261 gaggcggggg tcctccggcg ggactccggc tcgcccgccg tccgtgtcca cttcgatcag
     4321 gacggggaag accgtgccca gcacgtccgc ctcccgagcc acggcctcgg ccatctcgag
     4381 gctgtcgacg atgatcgtca cgtcggcccc gcgctgctgg agcgtagcga cattcggaag
     4441 cttggaaggc acgatcccga ccgcgtagag gatgtcggtg aagccgtgat ccaggaaata
     4501 gtctgcttcc ctgagggtgg agacggtgat cccgcccggc tgcccctcga ccgccagccg
     4561 aaccacgtcg atcgacttgg cggtcttgac gtgcggcctg agcggcaccc ccagcccggc
     4621 gagccggcgc cgcatccgct cgatattgtg cctcaggcgg gcggggtcca ccaccaaagc
     4681 gggcgtctcg agatcgctga tctgcatccg ggccctatat caagggccga tcccggtcgg
     4741 ccaccctaat cccgtctctt cggcccggcc tatgcctgcg gccgggcggc ttccgcggga
     4801 tcctcgagca ccggcgcgaa ttcctgaagg acgatggtcc cgatccagat cgccagcgtc
     4861 gccagggtcc tgtgcagatc ggcttccggg aggccccggg gctgcaaggc cggaatgttc
     4921 cacatcgcga cgatgagcac cgcataagcc gccacgaacc cgaccgcgaa gccgcgacgc
     4981 tccgacgtcg agatcattcg aagggccatc accgttccgg ccaggaacag gatgaaagag
     5041 gtgtactgcc gcaaggtgag tgagttcaga gtccccagat tcgccccgag cagggctccg
     5101 aagaacaggt tgactccgtc gatcgtcccg gcgcgctccc ggct
//
LOCUS       DASWNU010000556         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_438183, whole genome shotgun sequence.
ACCESSION   DASWNU010000556 DASWNU010000000
VERSION     DASWNU010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438183"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1785)
                     /locus_tag="VGB57_11000"
     CDS             complement(<1..>1785)
                     /locus_tag="VGB57_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131921.1"
                     /translation="AAAREREAAKYALSTSTAQIGAFGDMAAAAKGFFKEKSKGYQVM
                     LAAEKVFRAFEFAMSVRAMVQDVAETISSVANSGARATAAGAEGVANQSKLPFPFNIA
                     AMAATAAALVAAGIAVLGSGGGSGSKPAPTNTGTGTVLGDPAAQSESIKNAINALKDV
                     DTLTNTYARQMASSLRSIESQIGGIASLVVRQGDLNASAGVTEGFNKNFIGSALSKIP
                     LIGGMLGGLFGTKTTVLASGLYAGSQSLGSILNGGFDASYYSDIQKKKKLFGITTSNK
                     TSTQFAAADGALEAQFALLLRSFRDAIVAAAGPLGAATAEIENRLNSFVVNIGKIDLK
                     GLTGAEIQEKLTAIFGAAADSMAAAAFPGLEQFQKVGEGAFETLVRVASTVEAVSGTL
                     DLLGQSAQQMGLAAKMGLADQFDSVSALTDAAEAYFQAFYTEQEQAAAKTAQLAKVFA
                     SLGLAMPSTLAAFRELVEAQDLTTAAGQATYATLLKLAPAFADLQSALEGAKSAADIA
                     SERADLQRQLLQLQGDTAAIRALELAKVDASNRALQLQVWAVQDAQEAAKAADELRKA
                     WQSVGDTIMDEVRRIRGLSDTAGAGTFAS"
BASE COUNT          253 a          610 c          619 g          303 t
ORIGIN      
        1 gaggcgaagg tgccggcgcc ggccgtgtcg gacaggcccc ggatccgccg gacctcgtcc
       61 atgatcgtgt cgccgaccga ctgccaggcc ttccggagct cgtcggcggc cttagcggcc
      121 tcctgggcgt cctggacggc ccagacctgc agctgcagcg cccggttgct cgcgtcgacc
      181 ttggcgagct cgagggcccg gatggccgcg gtgtcgccct gcagctggag cagctgccgc
      241 tggagatccg cgcgctcgct ggcaatgtcg gccgcgctct tcgccccctc gagggcggac
      301 tgcaggtcgg cgaaggccgg cgcgagcttc agcagcgtgg cgtaggtcgc ttgcccggcc
      361 gcggtggtca gatcctgcgc ttcgaccaac tcgcggaaag cggccagcgt cgagggcatg
      421 gcgagcccga ggctggcgaa gaccttcgcc agctgggcgg tcttcgccgc ggcctgctcc
      481 tgctcggtgt agaaggcctg gaaataggcc tctgcagcgt cggtgagggc cgacacgcta
      541 tcgaactggt ccgcgaggcc catcttggcc gccaggccca tctgctgggc gctctggccc
      601 aggaggtcca aggtgccgct gacggcctcg accgtagagg cgacccgcac cagcgtctcg
      661 aacgcgccct cgcccacctt ctggaactgc tcgaggcccg ggaaggccgc ggccgccatg
      721 ctatcggccg cggcgccgaa gatcgcggtc agcttctcct ggatctcggc gccggtgagc
      781 cccttcaggt cgatcttgcc gatattgacg acgaagctgt tgagccggtt ctcgatctcg
      841 gccgtggcgg cgcccagcgg cccggccgcg gcgacgatcg catcgcggaa ggagcggagc
      901 agaagcgcga actgcgcctc gagcgcgcca tcggcggccg cgaattgcgt cgacgtctta
      961 ttgctggtgg tgatcccgaa gagcttcttc ttcttctgga tgtcgctgta ataggaggcg
     1021 tcgaagccgc cgttcaggat gcttccgagc gactgcgatc ccgcatagag gccgctggcc
     1081 aggaccgtgg tcttggtccc gaacaggccg ccgagcatcc cgccgatgag cgggatcttg
     1141 ctcagcgccg agccgatgaa gttcttattg aagccctcgg tgacgccggc cgaggcgttg
     1201 agatcgccct gccggaccac cagcgaggcg atcccgccga tctggctctc gatcgaccgg
     1261 agcgaagacg ccatctgacg ggcgtaagta ttcgtgaggg tgtcgacgtc cttcagggcg
     1321 ttgatcgcgt tcttgatgct ttcgctctgg gccgcggggt cgccgagcac ggtgccggtg
     1381 ccggtgttgg tcggcgccgg cttgcttccg ctaccgcctc cggatccaag gaccgcgatt
     1441 cccgcggcga ccagggcggc cgccgtggcc gccatagcgg cgatgttgaa cgggaacggc
     1501 agcttcgact gattggcaac gccctccgcg ccggccgcgg tggcccgggc cccgctgtta
     1561 gcgacggagg agatcgtctc ggcgacgtcc tggaccatgg cgcgcacgga catcgcgaac
     1621 tcgaaggcgc ggaatacctt ctcggccgcg agcatgacct gataaccctt gctcttctcc
     1681 ttgaagaagc ccttcgcggc cgcggccata tccccgaagg cgccgatctg cgctgtcgac
     1741 gtcgacagcg catatttggc cgcctcgcgc tcccgggcgg ccgcc
//
LOCUS       DASWNU010000557         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_384476, whole genome shotgun sequence.
ACCESSION   DASWNU010000557 DASWNU010000000
VERSION     DASWNU010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384476"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="metF"
                     /locus_tag="VGB57_11005"
     CDS             complement(<1..913)
                     /gene="metF"
                     /locus_tag="VGB57_11005"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY0131922.1"
                     /translation="MSSLAPGISHWREPLFADVAGDIGVSFEFFPPKSEKMEETLWAS
                     VKTLEPLAPRFVSVTYGAGGSTRERTHNTVVRIQQETPLNAAAHLTCVEASRSEIDEV
                     ALGYWQAGIRHVVALRGDPPEPGKGFQARPDGYANAAELVAGLKRVAPFEISVAAYPE
                     CHPDSPTRAADLDNLKRKIDSGADRAITQFFFSPECFFRFRDAAAAAGIDAELVPGIL
                     PVSNVAQTRKFAAMCGAAIPLWMDKLFEGLDDLPAARQLIAATVAAELCGQLYSGGVR
                     QFHFYTLNRAELAYAICHLLGLRPKDRH"
     gene            complement(910..1878)
                     /locus_tag="VGB57_11010"
     CDS             complement(910..1878)
                     /locus_tag="VGB57_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0131923.1"
                     /translation="MFPPAPAAAEIFRSLGDPTRLRILALLRAMELSVGELAQVLGQS
                     QPRVSRHVRILIEAGLAERRKEGSWVFLSLADPIRVEPLFAALERWAELDGEDPWGPA
                     DAARLTAVRADRAAAAERYFAAHAGDWDEIRSLHIAESEVEAACARALGERPVGRLVD
                     IGTGTGRMLELFGPAAERALGVDRSPEMLRLARVKLAQAGLAAAELRQGDMYALPLAA
                     GSADTVILHQVLHYAQQPAAAVAEAARLLGPGGRLLIVDFAPHEREELRSRDAHLRLG
                     FADEVMLRYLDSAGLRGRVVEHLEGGELTVTLWEGERPQEKLKVVA"
     gene            2156..2446
                     /locus_tag="VGB57_11015"
     CDS             2156..2446
                     /locus_tag="VGB57_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131924.1"
                     /translation="MKKIVLTAAATLALGVAACTPANNDAGNNATDVNATELNAGDDV
                     NGAAVDVDANGAAAVSTTDNALDSIGDTAANGASAVGNAATDAANTVSNAVD"
     gene            2561..2815
                     /locus_tag="VGB57_11020"
     CDS             2561..2815
                     /locus_tag="VGB57_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131925.1"
                     /translation="MKKYGAMAALLACASLVAACGREDDGSPTAGENEQLNNISEVLD
                     ASPDSLVAEDPSLGNGEAGATGELPVVGDEAANDSAGNGM"
     gene            complement(2953..>4443)
                     /locus_tag="VGB57_11025"
     CDS             complement(2953..>4443)
                     /locus_tag="VGB57_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131926.1"
                     /translation="LRAAALLSLVLALPVSADAARTGSPASRLSLAACDIPKHEENNP
                     APGETIAVRCGTFAVPENRTAGRGRMLPLKVIVIPSLAPRAATKAPIFYLSGGPGQAA
                     TEATWLAGSWHRDEHDVVLVDLRGTGEGTALDCPSGRSDDDPQSFLRPYLSQGTGFAA
                     CRDELSKRADLTQYTTTIAMMDVDELRQALGHERIAIEGGSYGTRAAMEYIRLFPGRV
                     HSAVLYSLVPVENRAPLFHARAAQRAYDLMVGQCEAEAPCRAAYPDLKSHASTIMRRL
                     EAQPARVTIPNPATGQTTEVTLTASGFADALRVMLYGIENERRVPLLLSRALAGDYRP
                     FATAGLESNRGFSKAIRTGLLLSFTCAEDTWRIRPEEVGRESAGSFLGSVRVRAQMAA
                     CSVWPKGKVPAGYYRPPVSRVPVLLVSGNLDPVTPPEWGEIARRRFPNSVHAVVPGGT
                     HTPTNACIEGLARTLFATGSVKGLDPGCVAREPIPPFALPEGGAAR"
BASE COUNT          738 a         1503 c         1569 g          633 t
ORIGIN      
        1 catgtcgatc cttcggtcgg aggccgagca gatggcagat cgcataagcg agctcggcgc
       61 gattgagcgt gtagaaatgg aactggcgga ctccgccgga ataaagctgg ccgcaaagct
      121 cggcggcgac ggtcgcggcg atgagctggc gggcggcggg gaggtcgtcc aggccctcga
      181 agagcttgtc catccaaagc gggatggcgg cgccgcacat cgccgcgaac ttgcgggtct
      241 gggcgacgtt ggagacgggc aggatgcccg gcaccagctc ggcgtcgatc ccggccgcgg
      301 cggcggcgtc gcggaatcgg aagaagcatt ccggcgagaa gaagaactgg gtgatcgccc
      361 ggtcggcgcc ggagtcgatc ttgcgcttga ggttgtcgag gtcggccgcc cgggtcggcg
      421 aatccggatg gcattccgga taggccgcga ccgaaatctc gaagggcgcg acccgcttga
      481 gcccggcgac cagctcggcc gcgttggcat agccgtcggg acgggcctgg aagcccttgc
      541 ccggctcggg cggatcgccg cgaagggcga cgacgtgtcg gatcccggcc tgccaatagc
      601 caagggcgac ctcatctatc tcgctccggc tcgcctcgac gcaggtgagg tgggcagcgg
      661 cgttgagcgg cgtctcctgc tgaatccgga cgacggtgtt gtgggtgcgc tcgcgcgtgg
      721 acccgcccgc gccgtaggtg accgacacga aacgcggcgc gagcggctcg agagtcttca
      781 ccgaggccca aagcgtctcc tccatcttct ccgacttggg cgggaagaat tcgaacgata
      841 cgccgatgtc gccggcgacg tccgcgaaga gcggctcgcg ccaatgcgat attccgggag
      901 cgaggctgct catgcgacca ccttcaattt ttcctggggg cgctcgccct cccagagggt
      961 gacggtcagc tcgccgccct cgagatgctc gacgacccgg ccgcgaagcc ccgccgaatc
     1021 gagatatctg agcatgacct cgtcggcgaa gccgaggcgc agatgggcgt cccggctgcg
     1081 caattcctcg cgctcgtgcg gggcgaaatc cacgatgagg aggcgcccgc ccgggccgag
     1141 cagtcgcgcc gcctcggcga ccgcggcggc gggctgctgg gcgtaatgga ggacctggtg
     1201 gaggatcacc gtgtcggccg atccggcggc gagcggcaag gcgtacatgt cgccctggcg
     1261 gagctcggcg gcggcgaggc cggcctgggc aagcttgacc cgggcgaggc ggagcatctc
     1321 gggcgagcgg tcgactccca gagcccgctc ggcggcaggt ccgaacagtt cgagcatccg
     1381 gccggtgccg gtgccgatgt ccaccagccg gccgaccggc cgctccccga gcgccctggc
     1441 gcaggccgcc tcgacctcgc tctcggcgat atggagcgag cggatctcgt cccagtcgcc
     1501 ggcatgggcc gcgaaatagc gctcggcggc ggcggcgcgg tcggcccgga ccgcggtcag
     1561 gcgggcggcg tccgcggggc cccagggatc ctcgccgtcg agctcggccc agcgctccag
     1621 ggccgcgaac agcggctcga cccggatcgg atcggcgagg ctcaggaaca cccagctgcc
     1681 ctccttgcgg cgctcggcga ggcccgcctc gatgaggatc cggacatggc gcgagacccg
     1741 cggctggctc tggccgagga cctgggcgag ctcgcccacc gaaagctcca tcgccctcag
     1801 caaggcgaga atccggagcc gcgtcggatc gcccagcgag cggaagattt cggcggcggc
     1861 gggtgcgggc gggaacatgg gaataaggat ataaagatat ctttatatcc gggtcaaggg
     1921 aagatgccgc cgacacggtc gcggcgaccg gcgatcaccg tagaaatgcg gctaaactat
     1981 tgactctccg aaacgacctc ttcaagcttg ctgcttcggt tcggcgcaat tgcttaccaa
     2041 actagtagac ataccgccgc gcctccgata catccaatgc cgccggagcc ggcgtgggtc
     2101 ttcccgcgtc cggacctcgc ccggtaaagc ccttattctc caaggagaga atgttatgaa
     2161 gaagatcgtc ctcaccgccg ccgccaccct ggcgctcggc gtcgccgcct gcaccccggc
     2221 caataacgac gccggcaaca acgccaccga cgtcaacgcg accgagctga atgccggcga
     2281 cgacgtcaac ggcgccgccg tcgacgtcga cgccaacggc gccgccgcgg tcagcaccac
     2341 cgacaacgcg ctcgacagca tcggcgacac cgccgccaac ggcgccagcg ccgtcggcaa
     2401 cgcggccacc gacgccgcca acaccgtttc gaacgccgtc gactaagtcg aaggccagaa
     2461 ccggaaacga gagggtcgcg tgccaccggg cacgcggccc tttttatttg tgccgggctg
     2521 cggcaagctg gccggcgaac gcctatcggg agcaagacag atgaagaaat atggtgccat
     2581 ggccgcgttg ctcgcatgcg cctccctcgt cgccgcctgc ggccgcgagg atgacggcag
     2641 cccgaccgcg ggcgagaacg agcagctcaa caacatctcc gaagtcctcg acgcctcgcc
     2701 cgacagcctg gtcgcggaag acccttcgct cggcaacggc gaggccggcg ccaccggcga
     2761 gcttcccgtg gtgggcgacg aggccgccaa cgactccgcg ggcaacggga tgtaggaaat
     2821 tcctcccccg gatttcctgg ggaggaggac catgcgaagc atggtggagg ggccttgctg
     2881 actccgtaga acccctccac cgcgcttcgc gcggtccccc tccccgcgaa atcgcgggga
     2941 ggattgagaa gcctaccgcg ccgccccgcc ctccgggagc gcgaagggcg ggatgggctc
     3001 cctggcgacg cagcccggat cgagcccctt taccgagccg gtggcgaaca gcgtcctggc
     3061 gagcccttcg atgcaggcgt tggtcggcgt gtgggtgccg cccggaacca ctgcgtggac
     3121 gctgttcggg aatcggcgcc gggcgatctc cccccattcc ggcggagtca ccggatcgag
     3181 attgcccgag acgagcagca ccggcacccg cgacaccggc ggcctgtaat agcccgccgg
     3241 cactttcccc ttgggccaca ccgagcaggc ggccatttgc gcccttaccc gcacgctgcc
     3301 gaggaagctg cccgcgctct cccgcccgac ctcctccggc cggatccgcc acgtatcctc
     3361 ggcgcaggtg aaggagagta ggaggccggt acggatcgct ttcgagaagc cgcgattgct
     3421 ttccagcccg gccgtcgcga aaggccgata gtcgccggcc agagcgcgcg aaagcagcag
     3481 cgggacccgc cgttcgttct cgattccgta gagcatcacc cgaagcgcgt cggcgaagcc
     3541 ggaggcggtg agggtcacct cggtcgtttg cccggtcgcc ggattgggaa tcgtgacccg
     3601 tgccggctgc gcctcgaggc gcctcatgat cgtcgacgca tggctcttca gatcgggata
     3661 ggcggcgcgg cagggtgctt ccgcctcgca ctggccgacc atgagatcgt aggcgcgctg
     3721 ggcggcgcgc gcgtggaaga ggggcgcccg gttctcgacc ggtacgaggc tgtagaggac
     3781 ggcggagtgg accctgccgg ggaacagccg gatatattcc atcgccgctc gggtgccgta
     3841 agagccgccc tcgatggcga tccgttcgtg gccgagcgcc tggcggagtt catcgacgtc
     3901 catcatggcg atggtggtcg tatattgggt gaggtccgcg cgcttcgaca gctcgtcccg
     3961 gcaggcggcg aagccggtgc cctgggagag ataggggcgc aaaaagcttt gcggatcatc
     4021 gtccgacctc ccgctcggac agtccagcgc cgtcccctcg ccggtgccgc gcaggtcgac
     4081 cagcaccacg tcatgctcgt cccggtgcca ggatccggcg agccaggtcg cctcggtcgc
     4141 cgcctggccc gggccgccgg acaaatagaa gatcggcgcc ttcgtcgccg cccgaggcgc
     4201 gagcgagggg atgacgatca ccttcagcgg cagcattcgc ccgcggccgg cggtgcggtt
     4261 ctccgggacg gcgaaggtgc cgcagcgcac cgcgatggtc tcgcccgggg cggggttgtt
     4321 ttcctcgtgc ttggggatgt cgcacgccgc aagggacagg cgggaggcgg gacttccggt
     4381 ccgggccgca tccgcggaga cgggaagagc caggacgagg ctgagcaatg cggcggctcg
     4441 aag
//
LOCUS       DASWNU010000558         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_47747, whole genome shotgun sequence.
ACCESSION   DASWNU010000558 DASWNU010000000
VERSION     DASWNU010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47747"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VGB57_11030"
     CDS             <1..225
                     /locus_tag="VGB57_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY0131927.1"
                     /translation="IHRGIATRAFERRFGLADYVQVRSAELKDGMLAIELVREIPEEM
                     KPRKIEIGGSQPRVGDQRSENDNSAESQAA"
     gene            310..810
                     /locus_tag="VGB57_11035"
     CDS             310..810
                     /locus_tag="VGB57_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0131928.1"
                     /translation="MTMRDLIPWGRQENRAPALLGSEERSPFLSLRRDIDRLFDDFFQ
                     SPMLGLGRGAAMQWPNVEVSRTDSEIRITAEVPGMTEKDVELLVEDGVLTLRGEKKSE
                     SEDRDRGYSERFYGRFERRIALPSGIDEAKARAEFRDGVLSVTLPRSGEAERGRRIPI
                     NTGTRH"
     gene            912..1457
                     /locus_tag="VGB57_11040"
     CDS             912..1457
                     /locus_tag="VGB57_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0131929.1"
                     /translation="MFTDMRNGTAEDWAHIAAEHGKHIQSIAAQQIMHGLARLEAIEV
                     GFAANQLTHSLMAGTLARRSGASDEEVVAALCHDLGKLFSIPNHGPIAAEMLRPYVRD
                     DIYHAVYWHQEFQGRYYFEHLGKDPEGRERFKGESWYSFAEKLVDDWDAPAFDPDFEV
                     DPLESFEPEVTRVFSNPGAMV"
     gene            1594..2595
                     /locus_tag="VGB57_11045"
     CDS             1594..2595
                     /locus_tag="VGB57_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0131930.1"
                     /translation="MAIQPVRWPALKDWRDVLVRTWKEAGDDNVGLLAAGVAFYAFLA
                     FVPLLAATVLVYGLAAEPETVAEHIRQLFGVLPEDAAALIGDQLKSMTESPDAAKGWS
                     LVVAIALAIYGASKGSGAIVTALNIAYEVKESRGFVKTTLLSLLMTVGALVVLVIAAA
                     GISVMGWVEALFPGFPGWTHSLLQILFWAVAVVAVGFGLAAVYRYAPSRPDAPWMWIS
                     PGSAVATLLWLAGSLGFGLYVSRFGNYNATYGSLGGVVVFLTWLYLSAYIVLMGGEMN
                     SELERQQAKEAGVEAKPAAAGEAAGAAAPEPKPDPDRISWPMAVVGLVLARTGGGAR"
     gene            complement(2683..>3147)
                     /locus_tag="VGB57_11050"
     CDS             complement(2683..>3147)
                     /locus_tag="VGB57_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HEY0131931.1"
                     /translation="QPIEDPAALLSPDLLENEFVDWVQGSNLLKRAFREVAVIGGLVE
                     RHHPGKRKSGRQVTFSTDLIYDVLRRYEPDHLLLKAAWNDARGKMTEVGRLVRLLDRA
                     QQTMAHVELDRVSPMAVPVLVIVGRENTATGLAEDAILIEAESLAATAMRLD"
BASE COUNT          509 a         1041 c         1097 g          500 t
ORIGIN      
        1 atccatcgcg gcatcgccac ccgcgccttc gagcgccgct tcgggcttgc cgactacgtg
       61 caggtccggt cggccgagct gaaggacgga atgctcgcga tcgagctggt gcgcgagatt
      121 ccggaggaga tgaagccccg gaagatcgag atcggcggat cgcagcctcg ggtcggcgac
      181 cagcggtccg aaaacgacaa cagcgccgag agccaggccg cctgaccggt ccggcaatcg
      241 ggcaggccgc gccggcgccc ggcgccgggc ggccatttca accctgtaag tcactaaaag
      301 gaggatatca tgaccatgcg tgatctcatc ccgtggggcc ggcaggagaa ccgcgccccc
      361 gccttgctcg gctccgagga aaggagcccg ttcctgagcc tgaggcgaga catcgacagg
      421 ctgttcgacg acttcttcca gtccccgatg ctgggcctgg gtcgaggcgc ggcgatgcag
      481 tggccgaacg tcgaggtcag ccggaccgac agcgagatcc ggatcaccgc cgaggttccg
      541 ggaatgaccg agaaggacgt cgagctgctc gtcgaggatg gagtgctcac tctgcgcggt
      601 gagaagaaga gcgagagcga ggaccgcgac cgcggctatt cggagcgctt ctacggccgc
      661 ttcgagcgcc ggatcgccct cccctccgga atcgacgagg cgaaagcgag agcggagttc
      721 cgcgacggcg tgctcagcgt caccctgccg cggagcgggg aggccgaacg cggacgccgg
      781 atcccgatca acacgggcac tcggcactga tcctcggcca tgggcgaagc ggctccggct
      841 ccggccgggg ccgcttttcg ttgaaagcgc gggcttcggg gcgtaatcca tcggcgacag
      901 gagacacgcc catgttcacc gacatgagga acggcacggc cgaggattgg gctcatatcg
      961 ccgccgagca cggcaagcac atccagagca tcgctgcgca gcagatcatg catggcctgg
     1021 cccggctcga ggcgatcgag gtcggcttcg cggccaacca gctcacccac tcgctgatgg
     1081 ccggaaccct cgcccgccgc tccggcgcca gcgacgagga ggtggtggcg gccctctgcc
     1141 acgatctcgg caagctcttc tcgatcccca atcacggccc gatcgcggcg gagatgctca
     1201 ggccctacgt gcgcgacgac atttaccacg ccgtctactg gcaccaggaa ttccagggcc
     1261 gctattattt cgagcacctc ggcaaggatc cggagggccg cgagcggttc aagggtgaga
     1321 gctggtattc cttcgccgag aagctggtcg acgactggga cgctccggcc ttcgatccgg
     1381 atttcgaggt cgacccgctt gagagcttcg agccggaggt gacccgggtg ttttccaatc
     1441 cgggggcgat ggtctgactt cgcgtcgccc cagcgaaggc tggggccccg gcgggtgaag
     1501 aacgcaggtg cctttcaccc gctgggatgc cagccttcgc tggcatgacg gttttgcggg
     1561 aaccggcgcc cttccgcccg gttaggggcc gtcatggcaa tccaacccgt tcgctggccc
     1621 gcgctcaagg attggcggga cgtcctcgtc cgcacctgga aggaagcggg cgacgacaat
     1681 gtcggcctgc tcgccgccgg agtcgccttc tacgccttcc tggccttcgt tccgctgctc
     1741 gccgcgacgg ttctggtcta cggactcgcc gccgagccgg agacggtcgc cgagcatatc
     1801 cgccagctgt tcggggtcct tcccgaggat gcggcggcac tgatcggcga ccagctcaag
     1861 agcatgaccg aatcgccgga cgccgcgaag gggtggagcc tcgtggtggc aatcgcgctc
     1921 gcaatctacg gcgccagcaa gggctcgggc gcgatcgtca ccgccctcaa catcgcctat
     1981 gaggtgaagg agagccgcgg cttcgtcaaa accaccctgc tgtcgctgct catgacggtc
     2041 ggagcgctcg tcgtgcttgt gatcgccgcg gccgggattt cggttatggg atgggtcgag
     2101 gccctgttcc ccggctttcc gggctggacg cacagcctgc tccagatcct gttctgggcg
     2161 gtggccgtcg tggcggtggg tttcggcctc gccgcggtct accgctacgc ccccagccgg
     2221 ccggacgcgc cgtggatgtg gatcagcccg ggctccgccg tcgccacctt gctctggctc
     2281 gccggaagcc tcggcttcgg cctctacgtc tcgcgcttcg gcaattacaa cgcgacctac
     2341 ggctcattgg gcggagtcgt cgtcttcctc acctggctct acctcagcgc ctatatcgtc
     2401 ctgatgggcg gggagatgaa ctccgagctc gagcggcagc aggcgaagga ggcgggggtg
     2461 gaggcaaagc ccgccgccgc cggggaagcg gcaggcgcgg cggcacccga gccgaaaccc
     2521 gatccggacc gcatcagctg gcccatggcg gtggtggggc tggttctggc gcgaacggga
     2581 ggaggcgcca ggtaacgtca tcccggcgaa agccgggacc tcaggacgca aaggcgcagc
     2641 gggtctcccc caggtcgcgg ctttcgccgg gatgacgggc ggtcaatcca gcctcatcgc
     2701 ggtcgcggcc aggctctccg cctcgatcag gatggcgtcc tcggcgaggc cggtcgccgt
     2761 attctcccgt ccgacgatga ccagcaccgg caccgccatc ggcgagaccc ggtcgagctc
     2821 gacatgggcc atcgtctgct gcgcccggtc gagcagccgg acgagccggc cgacctcggt
     2881 catcttgccc cgggcgtcgt tccaggcggc cttgaggagg agatggtcgg gctcgtagcg
     2941 gcggagcacg tcgtagatga ggtcggtcga gaaggtgacc tgccgccccg acttgcgctt
     3001 gccgggatgg tggcgctcga cgaggccgcc gatcactgcg acctcgcgga aggcgcgctt
     3061 gaggaggttc gagccctgga cccagtcgac gaactcgttc tcgagcaggt cgggggagag
     3121 cagggcggcg ggatcctcga tcggctg
//
LOCUS       DASWNU010000559         5034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_152550, whole genome shotgun sequence.
ACCESSION   DASWNU010000559 DASWNU010000000
VERSION     DASWNU010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152550"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1075
                     /locus_tag="VGB57_11055"
     CDS             2..1075
                     /locus_tag="VGB57_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEY0131932.1"
                     /translation="MLRAPGTPLSIEQATLDAPGPREVLIRTAACGLCHSDLHFIEGT
                     YPHPLPCIPGHEAAGIVEAVGSEVRTVKVGDHVVTCLSAFCGHCEYCVTGRMALCPGG
                     DTRRSKGAAPRITAGDGSAVNQMLNLSGFAEAMLVHEHACVAIDKDMPLDRAAVIGCA
                     VTTGAGTIFNACKLVPGETVAVIGCGGVGLATINAARIAGAGRIIAADPLPEKRELAR
                     KLGATDVVDPMEEGAGARIVEMTRGGVDHAIEAVGRPASAALAVKSLRRGGTATILGM
                     MPLDQKVGLGAMDLLSGKKLQGAFMGANRFPVDIPRLVDFYLRGLLDLDSIVAETIPL
                     ERVNEGFERMKRGDAARSVVVFD"
     gene            1176..1949
                     /locus_tag="VGB57_11060"
     CDS             1176..1949
                     /locus_tag="VGB57_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0131933.1"
                     /translation="MSLFDLTGKVAVITGSSRGIGRAVAEAMADQGAKVVISSRKAEA
                     CQEVADAINAKHPDAAIVVAASISSKEALQNLVDETRRRLGRIDILVCNAASNPYYGP
                     MGGITDDQFRKILENNVIANHWLIQMVAPEMIERRSGSIIIVSSIGGLKASAVIGAYN
                     ISKAADFQLARNLAAEFGPHQVRVNCIAPGLIRTDFARALWENPDTLKMVTAHTPLQR
                     IGEPEEIAGAAVFLASDASTFVTGQAIVVDGGSTTGVGL"
     gene            2042..2830
                     /locus_tag="VGB57_11065"
     CDS             2042..2830
                     /locus_tag="VGB57_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0131934.1"
                     /translation="MMRLADKIAIVTGAGSGIGRASAIRFAAEGARVICADLKGQDET
                     AGEIGEAAIGIQADAGNEDDVHDLVQAALQYHGTLDIFFANAGISGGLASIFEQGVED
                     WQEILRINLIGPSLAIKHSAPAMKENGRGSIVCTASVAGIRAGAGGPAYSASKAGVIN
                     LVRVAATQLAGADIRVNAICPGLIETGMTRSVYEWARAAGKEDRIGHLNPLKRGGIPE
                     EIAAAALFLASDEASYVNGHALVVDGGLSISHPYNHQEYGRTAV"
     gene            3113..3496
                     /locus_tag="VGB57_11070"
     CDS             3113..3496
                     /locus_tag="VGB57_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131935.1"
                     /translation="MGTEVASGLCVTRGVLTSPDSTFIWDVVENNGNLKVKVRYGGGT
                     DNSNAQTATIPAGSTAAVDISDGTNPDISVIGQVSAFWSDGNKSYVVVFSGQMVRPNV
                     NNITSSDQNGKAADSHPVIVYSYMK"
     gene            complement(3639..>5034)
                     /locus_tag="VGB57_11075"
     CDS             complement(3639..>5034)
                     /locus_tag="VGB57_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY0131936.1"
                     /translation="GLPLPFLLYAAGGEPDARYGLARFTAPGFAQQGLSRASDFDRDG
                     YSWFTPQRDWAPFDESRHPFVLDLPDNGVDEDGYGGDFRFEGGPASFPAPVIPDRKHV
                     VLVVLESARGDSVGKMVDGRPLTPVMNALVRDGSYTREAYSHVGFTTESLKSLFGGAL
                     APRPGAPSLFRDFRANGYRIGVFSGQAERFGGIAETAGMRESADVFIDAETLKSERAF
                     SFAATASLLLDGRKVLRAFDGAFGSAVGWRRPTFAYLNFQEAHFPYAHPGMPRILPGP
                     PIPRGEISEANKLWVARTYWNAIAYDDWLVGQLVERLKRLGVWEDTLLVVTADHGESL
                     FDDAFLGHGHVINRQQTQIPLILSRPGISIPQPIGLAGYRSLILRSLGAKVPERQGPV
                     FQHVSQLGRPAAIGLVGAGQVWTTFQLETEEVGFSDSRRRVRYSDLKPGSSEKKRADS
                     VIDEWNRQRWLARD"
BASE COUNT          925 a         1752 c         1594 g          763 t
ORIGIN      
        1 gatcctccgc gcccccggca ccccgcttag catcgagcag gcgacgctcg acgcccccgg
       61 cccgcgcgag gtcctcatcc gcaccgccgc ctgcggcctg tgccattcgg acctccactt
      121 catcgaaggc acctaccccc accccctccc ctgcatcccc ggccacgagg cggccggaat
      181 cgtcgaggcg gtcggaagcg aggtgcgcac ggtgaaggtc ggcgaccatg tcgtcacctg
      241 cctctccgcc ttctgcggcc actgcgagta ttgcgtgacg gggcggatgg cgctctgccc
      301 cggcggcgac acgcgtcggt ccaaaggggc ggcgccgcga atcacggccg gagacggaag
      361 cgccgtcaac cagatgctca acctctccgg cttcgccgag gcgatgctgg tccacgaaca
      421 tgcctgcgtc gccatcgaca aggacatgcc gctcgaccgc gccgccgtga tcggctgcgc
      481 ggtgacgacc ggcgccggca ccatcttcaa cgcctgcaag ctggtgccgg gcgagacggt
      541 ggcggtgatc ggctgcggcg gagtgggcct cgccacgatc aacgccgccc ggatcgccgg
      601 cgccggccgc atcatcgccg ccgatccgct ccccgaaaag cgcgagctcg cccgcaagct
      661 cggagcgacc gatgtcgtcg acccgatgga ggaaggcgcc ggcgcccgga tcgtcgagat
      721 gaccagaggc ggagtagacc acgccatcga ggcggtcggc cgccccgcct cggccgcgct
      781 cgccgtcaag tcgctccgcc gcggcggaac cgcgaccatc ctcggaatga tgccgctcga
      841 ccagaaggtc ggcctgggcg cgatggacct gctctccggc aagaagcttc agggcgcctt
      901 catgggcgcc aaccgcttcc cggtcgacat cccccgcctc gtcgatttct acctgcgcgg
      961 gctgctcgac ctggattcga tcgtggcgga gaccatcccg ctcgagcggg tcaacgaagg
     1021 cttcgaaagg atgaagcgcg gcgacgcggc acgaagcgtg gtggtatttg actagcaacc
     1081 gtcatcccgg cgaaagccgg gacctcgggg agacccgccg agacttctcg tcctgaggtc
     1141 ccggctttcg ccgggatgac gtgaaaggaa accgcatgtc cctcttcgat ctcaccggca
     1201 aagtcgccgt cataaccggc tcctcgcgcg gcatcggccg ggccgttgcc gaggcgatgg
     1261 ccgatcaggg cgcgaaggtg gtcatctcca gccgcaaggc cgaggcctgc caggaggtcg
     1321 cggacgcgat caatgcgaag catccggacg cggcaatcgt cgtcgccgcc tcgatctcct
     1381 ccaaggaagc gctccagaac ctcgtcgacg agacccgccg ccggcttggc cggatcgaca
     1441 tcctcgtctg caacgccgcg tccaacccct attacggccc gatgggcggc atcaccgacg
     1501 accagttccg caagatcctc gagaacaacg tcatcgccaa ccactggctg atccagatgg
     1561 tcgcccccga gatgatcgag cgccgctccg gctcgataat catcgtcagc tcgatcggcg
     1621 gcctcaaggc ctccgccgtg atcggcgcct acaacatctc caaggccgcc gacttccagc
     1681 tcgcccgcaa cctcgccgcc gagttcggcc cccaccaggt ccgggtcaac tgcatcgccc
     1741 ccggcctgat ccggaccgac ttcgcccgcg ccctgtggga aaatcccgac accctgaaga
     1801 tggtgaccgc gcacacgccc ctgcagcgta tcggcgagcc ggaggagata gcgggcgcgg
     1861 cggtcttcct tgccagcgac gcctcgacgt tcgtcaccgg tcaggcgatc gtggtagacg
     1921 gggggagcac caccggcgtc ggcctctgac cgtcatcccg gcgaaagccg ggacctcggg
     1981 gagacccgcc gagccttttc gacctgaggt cccggcttcc gccgggatga cggaggagca
     2041 aatgatgaga ctcgccgaca agatcgccat cgtcaccggc gccggctccg gcatcggccg
     2101 tgccagcgcg atccgcttcg ccgccgaggg cgccagggtg atctgcgccg acctgaaggg
     2161 ccaggacgag accgcgggcg aaatcggcga agcggcgatc gggatccagg ccgatgccgg
     2221 caacgaggac gacgtccacg acctcgtcca ggccgccctc caatatcatg gcaccctcga
     2281 catcttcttc gccaatgccg gcatctcggg cggcctcgcc tcgatcttcg agcagggggt
     2341 cgaggactgg caggaaatcc tccgaatcaa cctgatcggc ccctccctcg ccatcaagca
     2401 cagcgccccg gcgatgaagg agaatggccg aggctcgatc gtctgcaccg ccagcgtcgc
     2461 cgggatccgc gccggagccg gcggccccgc ttattccgcg tccaaggccg gagtcatcaa
     2521 cctcgtccga gtcgccgcca cccagctcgc cggagccgac ataagggtga acgccatctg
     2581 ccccggcctg atcgagaccg gaatgacccg ctccgtctac gaatgggcgc gagcggccgg
     2641 caaggaagac cggatcggcc acctcaaccc gctgaagcgc ggcggaatcc ccgaggagat
     2701 cgccgccgcc gccctcttcc tcgcctctga cgaggccagc tacgtcaacg gccacgccct
     2761 ggtggtcgac ggcggcctct ccatctcgca cccctacaac caccaggaat atggacggac
     2821 ggcggtttga ggcagggagc ggcgcgcggc tgtgcgccgg caataaacga cggcgggatt
     2881 agctcgcgat tttcctccaa agtttattgt agccatgctg atctgcggtg caactcgccc
     2941 gcggaagtcg cagcgaatga agatcatcaa cttcgagtcg acaagcaaaa aatagcatgt
     3001 cactatgact gatactgaac gtggcgtatt cgtagcgtag cgtcgggcga agatagactc
     3061 ggcccgccca ccgcgcacag gtggcaattt acttgaaatt ggggaggttc atatggggac
     3121 cgaggtcgca agtggattgt gcgttacgag gggtgtgctt acgtcgccgg actcgacctt
     3181 tatttgggac gtggtcgaga ataacggcaa cctgaaggtc aaagtgcgct acggtggagg
     3241 caccgacaac agcaacgcgc aaactgcaac gatcccggcg ggaagcacgg cggctgtcga
     3301 catctccgac ggaaccaacc ccgacatatc cgtgatcggt caagtctcgg cattctggtc
     3361 ggacggcaac aagtcctacg tcgtcgtctt ttcgggccag atggtaagac ctaacgtcaa
     3421 caacataacc agcagcgatc aaaacggaaa ggcagcggat agccatccgg ttatagttta
     3481 ttcctatatg aaataaacga gagctgggtc tggcaccgat tgcggcggcg gggtaaatgg
     3541 gtgtcagacc ccgatcggag aggagcgcgg tcggcgcccc cgcgccagcc atctgtgccg
     3601 cgcccatttc cgcgtgcgcg ggactgacca aggctggcct agtcccgggc cagccacctc
     3661 tgccggttcc actcgtcaat caccgagtcg gcccgctttt tctccgagct gcccggcttg
     3721 aggtcggaat agcgcactcg cctccggcta tcgctgaacc cgacctcctc ggtctcgagc
     3781 tggaacgtcg tccacacctg ccccgccccc accagcccga tcgccgccgg ccttccaagc
     3841 tggctgacgt gctggaacac aggcccttgc cgctccggca ccttcgcccc gagcgagcga
     3901 aggatcagcg agcgatagcc ggcgaggccg atcggctgcg ggatcgatat tccaggccgg
     3961 ctcaggatca gagggatctg ggtctgctgc cggttgatga catgcccgtg gccgaggaag
     4021 gcgtcgtcga acagcgactc gccatgatcg gcggtcacca ccaacagcgt atcctcccag
     4081 acgccgagcc gcttcagccg ctcgaccagt tgaccgacca gccagtcgtc ataggcgata
     4141 gcgttccaat aggtccgagc cacccataac ttgttggcct cgctgatctc gccgcgcggg
     4201 atcggcggcc ccggcaggat cctcggcatg cccggatgag cataagggaa atgcgcttcc
     4261 tggaagttga gataggcgaa ggtcggccgc cgccagccta ccgccgagcc gaacgcgccg
     4321 tcgaaggcgc gaagcacctt cctgccgtcg agcagcagcg acgcggtggc ggcgaagctg
     4381 aaggcccgct ccgacttgag ggtctcggca tcgatgaaga cgtcggcgct ctcccgcatt
     4441 ccggcggtct cggcgatccc gccgaatctc tccgcctgcc cggaaaagac cccgatcctg
     4501 tagccgttgg cccggaaatc ccggaacaac gacggagcgc ccggccgcgg agcgagcgct
     4561 ccgccgaaca ggctcttcaa ggattcggtg gtgaagccga catggctgta ggcctcgcgg
     4621 gtgtagctgc cgtcgcgaac gagcgcgttc atcaccggag tcagcggccg tccgtccacc
     4681 attttaccga ccgagtcgcc gcgagcgctc tcgaggacga cgaggacgac gtgcttgcgg
     4741 tcgggaatga cgggcgccgg gaagctcgcc ggcccgcctt cgaagcggaa atcgccacca
     4801 tagccgtcct cgtcgacgcc gttatcgggc aggtcgagaa cgaacgggtg ccgcgattcg
     4861 tcgaacggcg cccagtcgcg ctgaggagtg aaccaggaat agccgtcgcg atcgaaatcg
     4921 ctcgcccggc tcagcccctg ctgagcgaag cccggcgcgg tgaagcgggc gaggccgtag
     4981 cgcgcgtccg gctcgccgcc cgccgcgtag agcaggaagg gcagcggcaa tccg
//
LOCUS       DASWNU010000560         4943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_152618, whole genome shotgun sequence.
ACCESSION   DASWNU010000560 DASWNU010000000
VERSION     DASWNU010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152618"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..499
                     /locus_tag="VGB57_11080"
     CDS             119..499
                     /locus_tag="VGB57_11080"
                     /inference="COORDINATES: protein motif:HMM:NF020472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1843 domain-containing protein"
                     /protein_id="HEY0131937.1"
                     /translation="MATPTGGQVRPYGVAISDALQDPNSTLENLTALRDQGRRELEET
                     GDLAGALEKLEAEIQSRSGQYARPGGGVQPLYGVVIYDALSDPQTTLDRLTELRDQGR
                     RQLEEQGDLPGSLERLENELSRRG"
     gene            520..714
                     /locus_tag="VGB57_11085"
     CDS             520..714
                     /locus_tag="VGB57_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1843 domain-containing protein"
                     /protein_id="HEY0131938.1"
                     /translation="MAEAARGPIPPYGNAISDALSDPNTSVEELIAHRDHAREVLAAQ
                     GDLRAALERLDDEIQRRGGN"
     gene            719..2176
                     /locus_tag="VGB57_11090"
     CDS             719..2176
                     /locus_tag="VGB57_11090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0131939.1"
                     /translation="MADAGGTRTEPVRIRSEADRRLRHPVHVVWELTLACNLRCAHCG
                     SRAGRPRSDELTTDEIRSIVAELAALGTREISLIGGEAYLRRDWLDIVRAIAESGIKC
                     GLQTGGRALTRAKIEAAVDAGLSSAGVSVDGTEEVHDRLRGVPGSFRQALTAIEEFAR
                     AGITPGCNTQVNRLSAPVLEETYEAIYAAGARLWQIQLTVPAGNAAEQVDLILQPWQI
                     PETYDTLARLFERGRKAGFRLFSGNNIGYFGPYEHLWRTMTDEPAYWDGCGAAESGMA
                     IEADGVVKGCPSLHKGEYGGGNVRDHGSLTEIWLAMQESVRPNVERPAWGFCGTCYYK
                     SVCKSGCTWTAGSLLGVPGNNPMCDHRARTLREAGLRERFEQVSPAPGEPFDQGLWRL
                     ALETLDGVELPESAYPASKQPKNPDGEILLCRGCGHYSFEGDDGCMKCGAALVAERPR
                     GDFAGPQEMEGVLGRLAEIDARHARKLAEIRAARS"
     gene            complement(2263..2451)
                     /locus_tag="VGB57_11095"
     CDS             complement(2263..2451)
                     /locus_tag="VGB57_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY0131940.1"
                     /translation="MNGPFTLDKANGKMMGVCAGLADVTGADATLIRLAAVLSLFVLG
                     PVAIILYVVAGCVAPERA"
     gene            complement(2949..>4943)
                     /locus_tag="VGB57_11100"
     CDS             complement(2949..>4943)
                     /locus_tag="VGB57_11100"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY0131941.1"
                     /translation="VGIVVAALVFFFLGGTEAIGFVIGALLSGAAGFIGMNISVRANV
                     RTAEAARTSLQRGLTVAFRAGAITGMLVAGLALLAISVFFYFLVDVADYAPNDRQVID
                     ALVSLAFGASLISIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGD
                     NVGDCAGMAADLFETYVVTVGATMVLIALLVDAGAEQMLALMSLPLIIGGVCILTSII
                     GTYFVRLGRKQSIMGALYKGFWLTAGLSIPAIFYVMWQTLGGDMNAVIGSAREAAGTD
                     VAADVARTAVGGGFTGWDIFWCSLVGLAVTGLIVWITEYYTGTNYRPVRSIAKSSQTG
                     HGTNVIQGLAISLESTALPTLVICVGIIVSYQLAGLIGIAFAATAMLALAGMVVALDA
                     YGPVTDNAGGIAEMSGMEEDVRTRTDALDAVGNTTKAVTKGYAIGSAALGALVLFGAY
                     TEDLKHYAGELGIGAGGIDFALSNPYVVVGLLLGALLPYLFGAMGMTAVGRAAGNVVL
                     DVREQFRTNPGIMDGTSRPNYARTVDLVTRAAIKEMIIPSLLPVLTPIAVFFAISAIA
                     GREQGFAALGALLLGVIVSGLFVAISMTSGGGAWDNAKKYIEDGNYGGKGSEAHKAAV
                     TGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAALAGGGG"
BASE COUNT          866 a         1562 c         1768 g          747 t
ORIGIN      
        1 cggggcgcag tcggcgcaat ctcgcggcgg gaaaaatttg aaccgtttcg ctaccgatct
       61 aatcgactcc gcgggcctgg taagcttatc acaggttacc aacaaaagga ggacttccat
      121 ggcgactccg acgggtgggc aggtcaggcc ctatggcgtt gcgatcagcg acgcgctcca
      181 ggatccgaat tcgactctcg aaaatctcac cgcattgcgc gaccagggcc gccgcgagct
      241 cgaggagacc ggggatctcg ccggcgccct cgaaaagctc gaggccgaga tccagtcgcg
      301 gagcggtcaa tatgcccggc ccggcggcgg cgtgcagccg ctctacggcg tggttatcta
      361 cgatgctctc agcgatccgc agacgacgct cgatcggctc accgagcttc gcgaccaggg
      421 ccgccggcag ctcgaggagc agggcgatct ccccgggtcg ctggagcggc tcgagaacga
      481 gctgagccgg cgcggttgaa ccaagggaag gacaagctca tggcagaagc agcgaggggc
      541 ccgatccccc cctacggcaa cgcgatatcc gacgcgctca gcgatcccaa cacgagcgtc
      601 gaggagctga tcgcccatcg cgatcacgcc cgcgaggtgc tcgcggccca gggcgacctg
      661 agggcggcgc tggaacggct cgacgacgaa atccagcgcc gcggcgggaa ctgacgtcat
      721 ggcggatgcc ggcgggactc gcacggagcc ggtccggata cgctccgagg cggatcgccg
      781 gctccgccac cccgtccacg tcgtctggga gctgacgctc gcctgcaatc tgaggtgcgc
      841 ccattgcggc tcccgcgccg gccggcctcg aagcgacgag ctcaccaccg acgagatccg
      901 ctccatcgtc gccgagctgg ccgcgctcgg cacccgcgag atcagcctga tcggcggcga
      961 ggcctatctg cgccgcgact ggctcgacat cgtccgcgcc atcgccgagt cgggaatcaa
     1021 gtgcgggctc cagaccggcg gccgggccct gacccgcgcc aagatcgagg cggccgtcga
     1081 cgccggcctt tcctcggcgg gagtctcggt cgacggcacc gaggaggtgc acgaccggct
     1141 tcgcggagtg ccgggcagct tccgtcaggc gctgaccgcg atcgaggaat tcgcccgggc
     1201 cgggatcact ccgggctgca atacgcaggt caaccggctg tcggcgccgg tgctcgagga
     1261 gacgtacgag gcgatctacg ccgccggcgc ccgattgtgg cagatccagc tcaccgtccc
     1321 cgccggcaac gccgccgagc aggtcgatct gatcctccag ccctggcaga taccggaaac
     1381 ctacgacacc ctcgcgaggc ttttcgaacg cggccgaaag gcgggcttcc gactcttctc
     1441 gggcaacaat atcggctatt tcggccccta cgagcatctc tggcggacga tgaccgacga
     1501 gccggcctat tgggacggct gcggcgccgc cgaaagcggg atggcgatcg aggccgacgg
     1561 ggtcgtcaag ggctgcccct cgcttcacaa gggcgaatat ggcggcggca acgtgcgcga
     1621 ccatggcagc ctcaccgaga tctggctggc catgcaggag tcggtccggc cgaacgtcga
     1681 acggcccgcc tggggctttt gcggcacctg ctattacaag tcggtgtgca aatcgggctg
     1741 cacctggacg gcggggtcgc tcctcggagt gcccggcaac aacccgatgt gcgaccatcg
     1801 cgcccgcacc cttcgcgagg cggggctgcg cgagcgcttc gagcaggtct cgccggcgcc
     1861 gggcgagccg ttcgaccagg ggttgtggcg gctcgcgctt gagaccctcg atggcgtcga
     1921 gctgccggag agcgcctatc cggcctcgaa gcagccgaag aatccggacg gcgagatcct
     1981 gctgtgccgc ggctgcggcc attattcgtt cgagggcgac gacggctgca tgaagtgcgg
     2041 cgcggcgctg gtggcggagc ggccgcgcgg cgatttcgcc gggccgcagg agatggaagg
     2101 ggtgcttggg cggctggccg agatcgatgc ccgccatgcg aggaagctgg cggaaatccg
     2161 ggcggctcgg agctagtcgt caccccggcg aaagccgggg cacggggaga cccgccgagc
     2221 ctcattcgtg ctgtggtccc ggcttgcgcc gggatggcgg gatcaggcgc gctcgggcgc
     2281 gacgcagccg gcgacgacgt agagaatgat cgcgaccgga cccagcacga acaggctgag
     2341 cactgccgcc aggcggatga gcgtcgcgtc ggctcccgta acgtcggcga ggccggcgca
     2401 gacgcccatc attttgccat tggctttgtc gagggtgaaa ggaccgttca tggcgccttt
     2461 ccttttcgtg atgtggcgat ttctcgcgat tatcggtgat tcctgaactt ccctgcccgt
     2521 gggccggacg gtcagatgat ctgaccggcc gggccgacgg cggcggagat gaacagggtc
     2581 gcggcgaaga tggcgccgac ggcggagatc gcgatgtgct gcaggctggc gaaattgaag
     2641 gtcattttaa gtctccctga attcggtcgc cggttgatcc gacgaccatg ccagagatgt
     2701 tgcagagacc gtgccaaaca gcagaaacgg cagaaattcg ccattttcta cctgggcggc
     2761 ggcattggcg cgaaatcttc ttttaaacta ttgggaattt tcaccgacaa ttggcggacg
     2821 caatcgaacg tgctcgcgtc cgcatccgga caggtgagcc gggccccggt cggtttccag
     2881 agcggcaacg aaaaaccccg cccggatcgc tccgggcggg gctcttcggt tcgatcaggc
     2941 cgggaagatc agccgccgcc gccggccagc gccgccagca gcagcaaagc gacgatgttg
     3001 gtgatcttga tcatcgggtt caccgccggg ccggccgtgt ccttgtaggg atcgccgacc
     3061 gtgtcgccgg tgacggccgc cttgtgggct tccgagccct tgccgccgta attgccgtcc
     3121 tcgatatatt tcttggcatt gtcccaggcg ccgccgcccg aggtcatcga gatggcgacg
     3181 aacaggccgg agacgatcac gcccagcagc agcgcgccga gggcggcgaa gccctgctcg
     3241 cgaccggcga tggccgagat cgcgaagaag acggcgatcg gggtgagcac cggcagcagc
     3301 gaggggatga tcatttcctt gatcgccgcg cgggtgacga gatcgacggt gcgggcgtaa
     3361 ttgggcctcg aggtgccgtc catgatgccg gggttggtgc ggaactgctc gcggacgtcg
     3421 agaacgacgt tgccggcggc gcggccgacc gcggtcattc ccatcgcccc gaagaggtaa
     3481 ggcagaaggg cgccgaggag caggccgacg acgacgtagg ggttggacaa agcgaaatcg
     3541 atgccccccg cgccgatgcc gagctcgccg gcataatgct tgaggtcctc ggtataggcg
     3601 ccgaacagca ccagcgcgcc gagggcggcc gagccgatgg catagccctt ggtcaccgcc
     3661 ttggtggtgt tgccgacggc gtcgagggcg tcggtgcggg tgcgcacgtc ctcctccatg
     3721 cccgacatct cggcgattcc gccggcattg tcggtgactg ggccataggc gtcgagggca
     3781 acgaccattc cggccagcgc cagcatcgcg gtcgcggcga aggcgatccc gatcaggccg
     3841 gccagctggt aggagacgat gatcccgacg cagatgacga gggtcgggag cgccgtcgat
     3901 tcgaggctga tcgcaaggcc ctggatgacg ttggtgccgt ggccggtctg cgaggatttg
     3961 gcgatcgagc ggaccgggcg atagttggtg ccggtataat attcggtgat ccagacgatg
     4021 aggccggtga cggcgaggcc gaccagcgag caccagaaga tatcccagcc ggtgaagccg
     4081 ccgccgacgg cggtgcgtgc gacgtcggcc gcgacatcag tgcccgccgc ttccctggcg
     4141 ctgccgatga cggcgttcat gtccccgccc agcgtctgcc acatcacgta gaagatggcg
     4201 gggatggaga ggccggcggt cagccagaag cccttgtaga gggcgcccat gatcgactgc
     4261 ttgcggccga ggcggacgaa ataggtgccg atgatcgagg tcaggatgca cacgccgccg
     4321 atgatcaggg gcaggctcat cagggccagc atctgttcgg cgcccgcgtc cacgagaagg
     4381 gcgatcagaa ccatggtggc gccgacggtg acgacatagg tctcgaacag atcggcggcc
     4441 attccggcgc agtcgccgac attgtcgccc acgttatcgg cgatgacggc cgggttgcgg
     4501 gggtcgtcct cggggattcc ggcctcgacc ttgccgacga ggtcggcgcc gacgtcggcg
     4561 gccttggtga agatgccgcc gccgagtcgg gcgaagatcg agatgagcga ggcgccgaag
     4621 gcgaggctga cgagggcgtc gatgacctgg cgatcgttcg gggcgtaatc ggccacgtcg
     4681 accaggaagt agaagaagac cgagatggcg agcagcgcca ggccggcgac cagcattccg
     4741 gtgatcgcgc ccgcccggaa ggcgacggtg aggccgcgct ggaggctggt gcgggcggcc
     4801 tcggcggtgc ggacgttggc gcggaccgag atgttcatcc cgatgaagcc ggcggctccg
     4861 gacagcagcg cgccgatgac gaagccgatg gcttcggtgc cgccgaggaa gaagaagacg
     4921 agggcggcga cgacgattcc gac
//
LOCUS       DASWNU010000561         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_514669, whole genome shotgun sequence.
ACCESSION   DASWNU010000561 DASWNU010000000
VERSION     DASWNU010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514669"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VGB57_11105"
     CDS             <1..477
                     /locus_tag="VGB57_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131942.1"
                     /translation="ISEQIASEGGDATEGQLPAPLNPGQEAELISESEASAPASAPQA
                     VKDVIAAANAIADTPYIWGGGHGSFESSGYDCSGAVSYALHGGGLLESPLDSTGLTTW
                     GEPGPGRWITVYGNSGHAWMVVAGLAFDTSGGAGPRWHSDWPNSPEGFIARHPAGL"
     gene            560..>1898
                     /locus_tag="VGB57_11110"
     CDS             560..>1898
                     /locus_tag="VGB57_11110"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0131943.1"
                     /translation="MDLEQRLEGMLEIERFEPPAEFRANALWSDPKIYAEAAADPEAW
                     WMAQAKELLDWEVEPSEGLDDSDPPFYKWFADGRLNASYNCLDRHLEAGNGERVAYHW
                     RGEEGEERDVTYADLHRDVQRFANALKDLNVGKGDVVGIYLPMIPEVVVAMLACARIG
                     AIHNVVFGGFSAESVRERMEFSEAKALVTVDGARRKGKTAPIKPQVDEHLGDLGTLET
                     IVVVRHTGIECPMEVERDVFYDEVMAAADPECPAEPMEAEHPLYILYTSGSTAKPKGI
                     LHTTGGYMAGVTATHRYVFDLKPETDVYWCAADVGWVTGHSYIVYGPLANGATSVMWE
                     GAPDYPHKGIWWELVERYKATILYAAPTAIRTFIKWGAEIPAEHDLTSLRLLGSVGEP
                     INPKAWLWYQQVIGGGRCPIVDTWWQTETGAIMISPLPGITATKPGSATTPFPG"
BASE COUNT          314 a          683 c          633 g          268 t
ORIGIN      
        1 atctccgaac agatcgcctc cgaaggcggc gacgccaccg aaggtcaact gccggcgccg
       61 ctcaaccccg gccaggaagc ggagctgatc tccgaaagcg aagccagcgc cccggcctcc
      121 gccccgcagg cggtcaagga cgtgatcgcc gccgccaacg cgatcgccga caccccttac
      181 atctggggcg gcggccacgg ctccttcgaa tcttccggct acgactgctc gggcgcggtc
      241 agctacgcgc tgcacggcgg cggcctgctg gaaagcccgc tcgactcgac cggcctcacg
      301 acctggggcg aaccgggccc cggccgctgg atcaccgtct acggcaactc cggccacgcc
      361 tggatggtcg tcgccgggct cgccttcgac accagcggcg gcgccggccc tcgctggcac
      421 agcgactggc cgaactcgcc cgaaggcttc atcgccaggc accctgccgg gctctagctc
      481 cgcgtcgttt ccacgctttt gggccctata ggggccccga ttgtggaaac gacgcagccg
      541 gctgggatag tttgcggcta tggatcttga gcagcgactg gaggggatgc tggagatcga
      601 gcggttcgag ccgccggcgg agttccgcgc gaacgcgctc tggtctgacc cgaagatcta
      661 cgcggaggcc gccgccgacc ccgaggcttg gtggatggcg caggcgaagg agctgctcga
      721 ctgggaggtc gagccgagcg aaggactaga cgactccgac ccgcccttct acaagtggtt
      781 cgccgacggg cggctcaacg ccagctacaa ctgccttgac cgccacctcg aggccggcaa
      841 cggcgagcgg gtcgcctacc actggcgcgg cgaggagggg gaggagcgcg acgtcaccta
      901 cgccgacctg caccgcgacg tccagcgctt cgccaacgcg ctcaaggacc tcaacgtcgg
      961 caagggcgac gtggtcggga tctacctgcc gatgattccc gaggtcgtcg tcgcgatgct
     1021 cgcctgcgcc cgcatcggcg ccatccacaa cgtcgtcttc ggcggcttct cggccgagtc
     1081 ggtgcgggag cggatggagt tctccgaagc caaggcgctg gtcaccgtcg acggcgcccg
     1141 ccgcaagggg aagacggcgc cgatcaagcc gcaggtcgac gagcacctgg gcgacctcgg
     1201 caccctggag acgattgtcg tcgtccgcca taccggcatc gagtgcccga tggaggtcga
     1261 gcgcgacgtc ttctacgacg aggtgatggc cgccgccgac cccgagtgcc cggcggagcc
     1321 gatggaggcc gagcacccgc tctacatcct ctacacctcc ggctcgaccg cgaagccgaa
     1381 ggggatcctc cacaccaccg gcggctacat ggccggggtc accgccaccc accgctacgt
     1441 cttcgacctc aagcccgaga ccgacgtcta ctggtgcgcc gccgacgtcg gctgggtcac
     1501 cggccactcc tacatcgtct acggcccact tgccaacggc gccacctcgg tgatgtggga
     1561 gggggcgccc gactacccgc acaaggggat ctggtgggag ctcgtcgagc gctacaaggc
     1621 gacgatcctc tacgcggcgc cgacggcgat ccgcaccttc atcaagtggg gcgccgagat
     1681 cccggccgaa catgacctca cctcgctgcg cctgctcggc tcggtggggg agccgatcaa
     1741 cccgaaggcc tggctctggt accagcaggt gatcggcggc ggccgctgcc cgattgtcga
     1801 cacctggtgg cagaccgaga ccggggcgat catgatctcg ccgctgccgg ggatcactgc
     1861 gaccaagcca ggctcggcga cgacgccgtt ccccgggg
//
LOCUS       DASWNU010000562         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_332343, whole genome shotgun sequence.
ACCESSION   DASWNU010000562 DASWNU010000000
VERSION     DASWNU010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332343"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VGB57_11115"
     CDS             complement(<1..806)
                     /locus_tag="VGB57_11115"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0131944.1"
                     /translation="MIAPLAVALGDPAGIGPEITAKAWERRVADSVPPFFAVGAPEAL
                     RAVWGGPLQVVAGPDEAAACFEGALPIIRIDSAGDTRPGQPTLEGARNSLEALELAVG
                     LTRSGASSALVTGPVSKAQLYAIGFSHPGQTEFVAERCGIAAEMVAMMLAGPTLRTVP
                     VTTHVALRDVPGLLTTELIVQRGRTTIRALRRLFGIERPRLAVAGLNPHAGEGGALGR
                     EEIDVVIPAIARLRDEGAEVTGPHAADTMFHGPKRATYDAALCLYHDQALV"
     gene            complement(816..>1865)
                     /locus_tag="VGB57_11120"
     CDS             complement(816..>1865)
                     /locus_tag="VGB57_11120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0131945.1"
                     /translation="RNLVDEVLQIQAAEQADIKITKAEIDQYFARYAQGQFKMTPDQM
                     AALLRSNGASPETLKRQIHGEMAWARLQSRKIEPFVNISEDEVTAVIDRLNAAKGTQE
                     FKVSEIFLSATPETAAETRANADRIVQQLRGGASFLAYARQFSEASTAAVGGDLGWVR
                     SAQLPEALAGVVQAIPVGSISDPIAIPGGFSIIQVADTRQVLTADPRDAVLSLKQMTV
                     KFAPGSPRNVVEEKVQGLLRAVQTMGGCGGAEEAAIPIGAEVVTNDQVRVRELPPALQ
                     QTLLALGLGQATPAFGSSDDRVSVLVLCGRDDPDPATNVSFEQVYNQIAENRVNLRAR
                     RFLRDLRRDAVVDYR"
BASE COUNT          273 a          634 c          670 g          288 t
ORIGIN      
        1 accagcgcct gatcgtgata gaggcagagc gcggcatcgt aggtcgcccg cttcgggccg
       61 tggaacatcg tgtccgcggc gtgcggcccg gtgacctcgg ccccctcgtc gcgcagccgg
      121 gcgatggccg ggatgacgac gtcgatctcc tcccggccca gggctccgcc ttcgccggca
      181 tgggggttga ggccggccac cgccagccgc ggccgctcga tcccgaacag ccgccgaagc
      241 gcccggatcg tcgtccgtcc gcgctggacg atgagctcgg tggtcaaaag ccccgggacg
      301 tcgcgaagcg cgacatgggt cgtgaccggc acggtgcgta aagtagggcc ggcgagcatc
      361 atcgccacca tctcggcggc gattccgcac cgctccgcga cgaactcggt ctggccggga
      421 tggctgaagc cgatcgcgta gagctgggcc ttggaaacag ggccggtgac gagcgcggaa
      481 ctcgcgcccg atcgggtaag cccgaccgcg agctccaggg cctcgagcga gttgcgggcg
      541 ccttcgaggg tcggctggcc cgggcgggtg tcgccggccg agtcgatccg gatgatcggg
      601 agcgcgccct cgaagcaggc ggcggcttcg tcggggccgg cgacgacctg gagcgggccg
      661 ccccagaccg cccgaagcgc ttccggagct ccgaccgcga agaacggcgg aaccgagtcc
      721 gcgactcgcc gctcccaggc cttggccgtg atctcgggtc cgatccccgc gggatccccg
      781 agcgcgacgg ccaggggtgc gatcaaggcg ccgcgctagc gatagtcgac caccgcatcg
      841 cgcctgaggt cgcgcaggaa gcgccgggcg cgcagattga cgcggttctc ggcgatctgg
      901 ttgtagacct gctcgaagct gacgttggtc gccggatcgg gatcgtcgcg gccgcacagc
      961 accaatacgc tcacccggtc gtcggaggag ccgaaggcgg gcgtggcctg gccgaggccg
     1021 agggcgagga gggtctgctg aagcgccggc ggcagctcgc ggaccctgac ctggtcgttg
     1081 gtgacgactt cggcgccgat cggtatcgcc gcctcttccg ccccgccgca gccgcccatg
     1141 gtctgaacgg cgcgaagcag accctgcacc ttctcctcga cgacgttcct cgggctcccc
     1201 ggggcgaact tgacggtcat ctgcttgagg ctgaggacgg cgtcgcgcgg atcggcggtg
     1261 agcacctgcc gagtatcggc gacctggatg atcgagaagc cgcccgggat cgcgatcgga
     1321 tcgctgatgc ttccgaccgg gatcgcctgg acgaccccgg ccagcgcctc gggcagctgg
     1381 gccgaacgca cccagcccag atcgccgccg accgccgcgg tcgaggcttc cgagaattgc
     1441 cgcgcataag cgaggaagga ggctccgccg cgcagctgct ggacgatccg atcggcattg
     1501 gcccgggtct cggcggccgt ctcgggagtc gccgaaagga agatttcgga gaccttgaac
     1561 tcctgggtgc ccttggcggc gttgagtcgg tcgatgacgg ccgtcacctc gtcttcgctg
     1621 atgttgacga acggctcgat cttccggctc tgcaggcgcg cccaggccat ctcgccgtgg
     1681 atctgcctct tcagtgtctc cggcgacgcc ccgttcgagc gcagcaaggc ggccatctga
     1741 tccggggtca tcttgaattg cccctgggcg tagcgggcga aatactggtc gatctcggcc
     1801 ttggtgatct tgatgtccgc ctgctcggcg gcctggatct ggagcacctc gtcgacgagg
     1861 ttgcg
//
LOCUS       DASWNU010000563         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_411027, whole genome shotgun sequence.
ACCESSION   DASWNU010000563 DASWNU010000000
VERSION     DASWNU010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411027"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..891
                     /locus_tag="VGB57_11125"
     CDS             148..891
                     /locus_tag="VGB57_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY0131946.1"
                     /translation="MATWSDPRTATTPQPTTTTGGDVAFDAGLRSHMLSVYNYMASGV
                     LLTGVVAMLFSRGGVESPAYQLFSQGGLLMWAVILAPLGLVMWLSFGINRISTGTAKT
                     LFWVYAVLMGLSLSSIFLVYEMGSIAQAFFATSAGFACLSLWGYTTKRDLSAMGTFLI
                     MGLFGLIVAMIVNFFMQSPAMDLAISALGVLIFAGLTAYDTQKIKSMYFAVAGTDFIG
                     KAAVMGALTLYLDFINLFLFLLRLFGDRR"
     gene            complement(943..1575)
                     /locus_tag="VGB57_11130"
     CDS             complement(943..1575)
                     /locus_tag="VGB57_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0131947.1"
                     /translation="MTETLTFYTNPMSRGRIVRWMLEEVGEPYETVLLDYASSMKGSE
                     YLAVNPMGKVPAIRHGDTVVTEAAAICAYLADMFPDKGLAPPSGNPRRGPYYRWLFFA
                     AGPVEAAVTGKSLGLLAPPEKAPMAGYGTFDDVMSTLEQAVKDCNYLCGDHFSAADVY
                     VGSQIGWGMMFGTIDRRPAFADYVGRLQSRPAAIRAREIDDALMPKQDPS"
     gene            1678..1881
                     /locus_tag="VGB57_11135"
     CDS             1678..1881
                     /locus_tag="VGB57_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131948.1"
                     /translation="MLRNLIGAAIGRRIAGRQNGVRGAMLGAAAPWIARRAFGPLGFA
                     VAGAWGAKKLYDRRRRGRVAPTV"
     gene            complement(1909..2298)
                     /locus_tag="VGB57_11140"
     CDS             complement(1909..2298)
                     /locus_tag="VGB57_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEY0131949.1"
                     /translation="MADSSDKQQLAEERTDYAEDRTVLANERTFAGWMRTGFASIAVG
                     VGFNLLTELEPVWVPKAIATAFLLIGIMIFVAAERRACAVVRRLHAHEVKTVRVGFLR
                     LITLTVILATIALIAALWLLEVKGSSG"
     gene            complement(2302..>2761)
                     /locus_tag="VGB57_11145"
     CDS             complement(2302..>2761)
                     /locus_tag="VGB57_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEY0131950.1"
                     /translation="TAARLPVPTFGKTGTTSDYRDAWFFGFAQDLVVGVWVGNDDNRP
                     MAGVSGGGIPARIWRSFMTQALGDGARAPVAEIPLQQADRVPTNEVQPSQAEPTPDQP
                     AAEPAEPRPLDDDSGEKPDDGAPPGPPTGAPPPEDALPPRPDPQPESGRL"
BASE COUNT          420 a          957 c          899 g          485 t
ORIGIN      
        1 cgggggccgg atcgcgacga acaggcggtg catggacggc ccgtagcgga acagcttcgc
       61 gcctgcgatc cggttttcct tgaatgtggg ggccgatccc acgatattgg gagcacggca
      121 ggggtgccgg caaaaggaga gttcaacatg gctacttggt ctgacccgcg gacggccacg
      181 acgccgcagc cgacgacgac caccggcggc gacgtcgcat tcgatgcggg cctgcgctca
      241 cacatgctga gcgtctacaa ttacatggca tcgggtgtgc tgctcaccgg cgtcgtcgcg
      301 atgcttttct cgcgcggcgg cgtggaatcg cccgcttatc agctattttc gcagggcggg
      361 ctgctcatgt gggctgtcat cctcgctccg ctcggcctgg tgatgtggct cagcttcggc
      421 atcaaccgga tctcgaccgg caccgcgaag accttgttct gggtctatgc ggtgctgatg
      481 gggctttcgc tctcgtccat ctttctcgtc tacgagatgg gctcgatagc ccaggccttc
      541 ttcgccacgt ccgccggctt cgcctgcctc agcctttggg gctacacgac caagcgcgac
      601 ctctcggcga tggggacctt cctgatcatg ggcctgttcg gcctgatcgt cgcgatgatc
      661 gtcaacttct tcatgcaatc gccggccatg gacctcgcga tcagcgccct cggcgtactg
      721 atcttcgcgg gcctcaccgc ctacgacacg cagaagatca agagcatgta cttcgcggtc
      781 gcggggaccg atttcatcgg caaggcggcg gtgatgggcg cgctcacgct ctatctcgac
      841 ttcatcaacc tgttcctgtt cctcctgcgg ctgttcggcg accgccgctg atccgcccga
      901 cggaggagca agcgaaggcc cggcgaaagc cgggcctttt ctttatgagg ggtcctgctt
      961 cggcatcagc gcgtcgtcga tctcgcgcgc ccgaatcgcg gccggccgcg actggaggcg
     1021 cccgacataa tcggcgaagg cgggcctgcg gtcgatcgtt ccgaacatca tcccccagcc
     1081 gatctgcgag ccgacataga cgtcggccgc ggagaaatga tcgccgcaca gatagttgca
     1141 gtccttcacc gcctgctcga gcgtgctcat cacgtcgtcg aacgtgccgt atccggccat
     1201 cggcgccttt tccggcggtg ccaacagccc gagcgacttg ccggtgacgg ccgcctcgac
     1261 cggccccgcg gcgaagaaca gccagcgata ataaggtccg cggcgcggat tgcccgaggg
     1321 cggcgccagc cccttgtccg ggaacatgtc ggccagatag gcgcagatcg cggccgcctc
     1381 ggtgaccacc gtgtcgccgt gcctgatcgc cggcaccttt cccatcggat tgacggcgag
     1441 atattcggag cccttcatcg agctcgcata atcgagcagc accgtctcat agggctcgcc
     1501 gacctcctcc agcatccagc ggacgatccg cccgcgagac atcgggttgg tgtagaaggt
     1561 cagggtctcc gtcatgctcg tgctccttgg gccgggccgc ggcagcgcgg gggaacattc
     1621 gcccgcgtgc agcgctcttc gcctcacatt gcaagacttt gacccgggag aacagccatg
     1681 cttcgcaacc tcatcggcgc cgccatcgga cgcaggatcg ccggccgcca gaacggcgtc
     1741 aggggcgcca tgctcggcgc tgcggcgccc tggatcgcgc gccgcgcctt cggcccgctc
     1801 ggcttcgccg tggccggggc ctggggcgcc aagaagctgt acgaccgccg ccgccgcggg
     1861 cgcgtcgcgc cgaccgtctg accatctgat cgaaaagagg ggagccgcct agccgctgct
     1921 ccccttcacc tcgagcagcc acaaggcggc gatgagggct atcgtcgcca gtatcactgt
     1981 gagcgtgatc agccgcagga agcccacccg cacggtcttc acctcatggg cgtggagccg
     2041 ccggaccacc gcgcaggcgc gccgttccgc tgcgacgaag atcatgatcc cgatcagcag
     2101 gaaggcggtc gcgatcgcct ttggcaccca gaccggttcc agctcagtca acagattgaa
     2161 gccgactccg accgcgatcg aggcgaaacc cgtccgcatc cagccggcga aggtccgctc
     2221 gttggcgagg acggtgcggt cctcggcata atcggtccgt tcttccgcca gctgctgctt
     2281 gtcgctgctg tcggccatgg ctcagagccg gccgctctcg ggctgcgggt cgggccgcgg
     2341 cggcagcgca tcctccggcg gcggcgctcc ggtcggcgga cccgggggcg cgccgtcatc
     2401 cggtttttcc ccgctatcgt cgtcgagcgg gcgaggttcg gccggctcag ccgccggctg
     2461 gtcgggcgtc ggctcggcct ggctcggctg gacctcgttg gtcggcaccc gatcggcttg
     2521 ttgcaacggg atctccgcga ccggcgcgcg ggcgccgtcg cccagcgcct gggtcatgaa
     2581 gctgcgccag atgcgggccg gtatgccccc gccgctgacc cccgccatcg gccggttgtc
     2641 gtcattcccg acccagaccc cgacgacgag atcctgggca aagccgaaga accaggcatc
     2701 ccgataatcg gacgtggtgc cggtcttgcc gaaggtcggc accggaagcc gcgccgccgt
     2761 g
//
LOCUS       DASWNU010000564         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_384647, whole genome shotgun sequence.
ACCESSION   DASWNU010000564 DASWNU010000000
VERSION     DASWNU010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384647"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1639)
                     /locus_tag="VGB57_11150"
     CDS             complement(<1..>1639)
                     /locus_tag="VGB57_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY0131951.1"
                     /translation="AGETLVFFATLLACQGLLFYGLYRLFRRLYGRRAGNALFHVDFL
                     CLAAGGGAALLIAKYQALSYFSDALSFQVVRNLGGGSLVQALLYSASEVGLVMVTIGG
                     AAAAYLGLRLLLRRRWRELAPLPDRTRLGCRQLAGAILAVPLILYAANRMEDVRSGLS
                     RFNSVYLISLLLNEATDFDRDGYGFYSWPTDGHPFDGSSHPYALDVPNNGIDEDGYAG
                     DLVMGEAAPHPRAPVRIAGERPHVILVVLESTRGDALGRRVSGIPVVPNLDALAAEGT
                     SVPGAYSHVGFTRDSMQTLFTGRLAPDGPRGSLFREFGANGYRTSVFSGQAEDFGGIA
                     DTVGMHDADIYVDASVLRDERAYSLGSVSSLSLDGRLLLREFERRLGRPEAWRRPNFV
                     YFNLQAAHFPYSFDGMERILPGTAISRREIGPSNKGRVETTYWNAVAYNDRLLGRIVQ
                     RLRQLGVWERSLLIVTGDHGESLFDDGFLGHGHMINRQQTHIPFVINRRLPVPGPVGL
                     ADMRAIILRAAGADVPADAAATPGRVFQFIGTLERPSS"
BASE COUNT          276 a          521 c          611 g          231 t
ORIGIN      
        1 gagctcgggc gctcgagcgt gccgatgaac tggaagaccc ggcccggagt cgcggccgcg
       61 tcggccggca cgtcggctcc ggctgcgcgc aggatgatag cgcgcatgtc ggccaggccg
      121 accggccccg gcaccggcag ccggcgattg atcacgaagg ggatatgggt ctgctggcgg
      181 ttgatcatgt ggccgtgacc gaggaagccg tcgtcgaaca gggattcgcc atggtcgccg
      241 gtgacgatca gcaggctgcg ctcccagacg ccgagctgcc gcagccgctg cacgatcctg
      301 ccgagcagcc ggtcgttata ggcgaccgca ttccaatagg tggtctcgac ccggcccttg
      361 ttcgagggcc cgatctctcg gcgggaaatg gcggtgccgg gcaggatccg ctccatcccg
      421 tcgaacgaat aggggaagtg ggccgcctgc aggttgaaat agacgaagtt cggcctccgc
      481 caggcctccg gccggcccag ccggcgttcg aactcgcgaa gcagcagccg gccgtccagg
      541 ctcaatgagc tgaccgatcc gaggctgtag gcgcgctcgt cgcggagcac cgaagcgtcc
      601 acgtagatgt ccgcatcgtg catgccgacc gtgtcggcaa tgcccccgaa atcttcggcc
      661 tggccggaga agacgctggt gcggtagccg ttcgctccga attcccggaa cagcgagccg
      721 cgcgggccgt ccggcgcgag gcggccggtg aacagggtct gcatcgaatc gcgggtgaag
      781 ccgacatggc tgtaggcgcc cgggacggag gtgccctccg cggcaagggc gtccagattg
      841 gggaccaccg ggatcccgct tacccgccgc ccgagcgcgt cgccgcgagt cgattcgagg
      901 acgacgagga tgacatgggg ccgctcgccg gcgatccgca ccggggctct ggggtgaggc
      961 gcggcctcgc ccatcacgag gtcgcccgca tatccgtcct cgtcgatgcc gttgttcggg
     1021 acgtcgagag cgtagggatg gctgctgccg tcgaaagggt ggccgtcggt cggccaggaa
     1081 tagaagccgt agccgtcgcg gtcgaagtcg gtggcttcgt tgaggagcag cgagatgagg
     1141 tagacgctgt tgaagcggct gaggcccgag cgcacgtcct ccatccggtt ggctgcgtag
     1201 aggatgagcg ggacggccag gatcgcgccg gccagctggc ggcagccgag gcgggtgcgg
     1261 tcgggcaggg gggcgagttc ccgccagcgg cggcgcagga gcagtctcag gccgagatag
     1321 gccgctgccg cgccgccgat cgtgaccatg accagcccca cctcgctggc gctgtagagc
     1381 agggcctgga ccaagcttcc gccgccgagg ttccgcacca cctggaagct gagcgcgtcg
     1441 ctgaaatagc tgagcgcctg atatttcgcg atcaggagcg ccgctccgcc gccggcggcg
     1501 aggcagagaa aatcgacgtg gaagagcgcg tttccggcgc ggcggccgta caggcggcgg
     1561 aacagccggt aaaggccgta gaaaaggagc ccctggcagg cgaggagggt ggcgaagaag
     1621 accagcgtct cccccgccg
//
LOCUS       DASWNU010000565         5150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_204111, whole genome shotgun sequence.
ACCESSION   DASWNU010000565 DASWNU010000000
VERSION     DASWNU010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204111"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGB57_11155"
     CDS             complement(<1..483)
                     /locus_tag="VGB57_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0131952.1"
                     /translation="MDAIEPDWDAAGVALDDQGWAILPRLLDGRACREAAALWREECR
                     FRSRVTMARHGFGRGEYRYFDTPLPGPVARLREGLYPPLAQVANRWRAALGRADRYPS
                     AHEDYLARCRAAGQARPTPLLLRYGPGDYNCLHQDLYGAEAFPLQVAILLSRPGDDFT
                     G"
     gene            679..1842
                     /locus_tag="VGB57_11160"
     CDS             679..1842
                     /locus_tag="VGB57_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131953.1"
                     /translation="MRPALSALLACALALPAAIPAACAAPGSAAEAEAGKLDVSWSIE
                     ADPDQRSKNLVQFALTRRTANSRWTHSNTRPLSSLTGLTAAQMESDSGAPVRFSLGRD
                     AGTISCEGVIRRWRGTGDCSFQPSSGFAAALAERGYGRAGDGQLFSMALHDLGYAFLD
                     ELKRQGYASATVADLVRAADHGVDLAYLKGMGGNGYRVGTLAALSNMRDHGVTPDYVS
                     ELARLGLADLSADEVVRLRDHGVTPDFAASFRTAGYEVPLDQLIRLRDHGVTPDYVRQ
                     LARYGIRGVPADQLVELRDHGVSPEFVGEAARQGYRFATAQLIRLRDHGVTSDYLGDL
                     KALGYSSLAAEEIVRLRSHGVTADFIRRVNASGGSRRSAAELVSLRAGGWAER"
     gene            2038..2745
                     /locus_tag="VGB57_11165"
                     /pseudo
     CDS             2038..2745
                     /locus_tag="VGB57_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(2742..3077)
                     /locus_tag="VGB57_11170"
     CDS             complement(2742..3077)
                     /locus_tag="VGB57_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0131954.1"
                     /translation="MSKKDISGISRVSEEGRPFVADWDDDDIPEADDTFFERAAFTRG
                     GKVVREGTGTLTNRGRPPLPEGTRKELVSLRLSPDILWWLRSTGPGWQSRLEDMLRER
                     MEAAMAAKD"
     gene            complement(3037..3318)
                     /locus_tag="VGB57_11175"
     CDS             complement(3037..3318)
                     /locus_tag="VGB57_11175"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0131955.1"
                     /translation="MKIEFDPVKRARVLRERGLDLAEAAELLLGECFTEADDRFDYGE
                     QRWVSYGLLRGEVVACVWVGQEEDRVRIVTMRKANRNEQERYFRYLAGF"
     gene            3404..>5150
                     /locus_tag="VGB57_11180"
     CDS             3404..>5150
                     /locus_tag="VGB57_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0131956.1"
                     /translation="MSAEAAPTRLTLYRDAGSRFGGAVRRAWGRRWVRILAYLAAIPV
                     ILYFLLWLLFARNLPSAETLLTYQPPLPTNVRGIDGEPVQTFARERRVELSFDEFPQT
                     LVDAFTSAEDKTFFTHGGIDYPGLVGAVGDYISKVGSGERARGGSTITQQVAKNLLLG
                     DEYSVTRKIKEAFLARRIESVLTKQQILALYLNQIFLGRNAYGVQAAARAYFDKDVEA
                     LELHEVAYLAILPKAPSNYDPQRRTQRALDRRNWVLAEMERNGRITAAQRAAAQAEPL
                     GTVRFANTSVRNVGGYFVEEVRRQLMTRWGEQTEKGPNGVYTGGLWVRTSLDVPKQKA
                     AEMALRDGLMRYDRGKGWRDPGLKVDVSGDWRGQLARAPFGVGYEDWRAAVVLSREGQ
                     SATIGFTDGSTGDLPAYAAAMPKRGTGTAAFNFLRPGMVIAVKRDGGIWSLRSIPEVK
                     GGMVVEEVATGRVLAMQGGFDARGDDFNRAVQAQRQPGSTFKPIVYSAALDNGMTPAS
                     IIVDGPFCANQGTPSQYCFRNFSGGHAGPQTMRWGLEQSRNLMTVRTASQIGMNNVVE
                     RARRMGVGDYRPFLSI"
BASE COUNT          831 a         1860 c         1663 g          796 t
ORIGIN      
        1 cccggtgaaa tcgtcccccg gccgcgaaag caggattgcg acctgcagcg ggaaggcctc
       61 ggcgccgtaa aggtcctgat gaaggcaatt atagtcgccg ggcccgtagc gcagaaggag
      121 cggggtcggc cgagcctggc cggcggcgcg gcagcgggcg agataatcct catgggccga
      181 ggggtagcga tcggcgcggc cgagcgcagc ccgccaccgg ttcgcgacct gggcgagcgg
      241 cggatagagg ccctcccgaa gacgggcgac cggccccggc agcggagtgt cgaaatagcg
      301 atattcgccc cggccgaaac cgtgccgcgc catcgtcacc cgacttcgaa agcggcattc
      361 ctcccgccac agtgcggcgg cttcgcggca ggcccgacca tcgagaaggc gagggagaat
      421 cgcccagccc tgatcgtcga gcgcaacgcc ggcggcgtcc caatccggtt cgatcgcatc
      481 catgaccccg tttggcgccc ggtggccggg ccggcactcc gcggcttgcg atcgatgtcc
      541 ggctgcggac aggagcgcgt ccgccagcgg acaggcggcc ggcaccgtcc ggccgaaacc
      601 ggcggttggg cgcgcatttc ggccatggcg cgctccttgc atcgacgggc ccgaacccca
      661 cagcgaaagc ttccctgcat gcgcccagcc ctttccgccc tcctcgcctg cgccctcgcc
      721 ctcccggccg cgattccagc cgcctgcgcc gccccgggct cggccgcgga ggccgaggcc
      781 ggcaagctcg acgtcagctg gtcgatcgag gccgatcccg accagcggtc gaagaacctg
      841 gtccagttcg ccctcacccg ccgaaccgcc aacagccgct ggacgcacag caacacccgt
      901 ccgctctcca gcctgaccgg actgaccgcc gcccagatgg aatccgacag cggcgccccg
      961 gtccgtttca gcctcgggcg agatgccggg acgatctcgt gcgaaggcgt catccggcgg
     1021 tggcgcggca ccggcgactg cagcttccag cccagctccg gcttcgccgc ggccctcgcc
     1081 gagcggggct atggtcgcgc cggcgacggc cagctcttct cgatggcgct tcacgacctc
     1141 ggctacgcct ttctcgacga gctgaagcgg caaggctacg catcggccac cgtcgccgac
     1201 ctcgtccgcg ccgccgatca cggcgtcgac ctcgcctatc tcaagggcat gggcgggaac
     1261 ggctacaggg tcggcaccct cgccgctctg agcaacatgc gcgaccacgg ggtgaccccc
     1321 gattacgtct ccgagcttgc ccggctcggc ctggccgatc tctccgcgga cgaagtcgtt
     1381 cggcttcgcg atcacggagt cacgcccgat ttcgcggcgt cgttccgaac cgccggctac
     1441 gaggtcccgc tcgaccagct catccgcctg cgcgatcacg gagtcacgcc cgattacgtc
     1501 cggcagctgg cccgctacgg aatccggggc gtgccggccg accagctcgt cgagcttcgc
     1561 gaccatggcg tctcccccga attcgtcggc gaggcggcgc ggcagggtta tcgcttcgcg
     1621 accgcccagc tgatccgcct gcgcgaccat ggggtgacgt ccgactatct cggcgacctc
     1681 aaggcgctgg gctattccag cctcgccgcg gaggagatcg tccggctgcg cagccacgga
     1741 gtcaccgcgg acttcatccg ccgggtcaac gcgtccggcg gcagccggcg aagcgcggcc
     1801 gagctggtgt cgcttcgcgc cggaggctgg gcggagcggt aggaagcggc aagggccgct
     1861 tgccaagcgg tgctggcgcc gggatagtga ccggcgatgg aagccttgct cgccctcctg
     1921 accgcgctcc tcatcgcgat cattccaccg ggcccgggag cccggcgcga cgagggcgtg
     1981 ccgatcgcgc tcgccgaccg aagcgatccc gcgccgggcc tgccgcgaat cgtcggcgat
     2041 ccggcccgtc cgctggtggt gctcgacgcc ggccatggcg gccacgatcc aggcgccgtc
     2101 tcgccattcg gcgcccgccg cgagaaggac gtcacgctcg ccatcgccac cgcgatccgc
     2161 gacgcgcttg ccgcttccgg gcgggtacgg gtggcgatga cccgggagga cgatcgctat
     2221 ctcgtcctca aggaccgcta cgagatcgcc cggaagatgc gcgccgcctt gttcatctcg
     2281 atccatgccg atgcggcgcc ggccaacgac ggcgcccgcg gcgccaccat ctacactttg
     2341 tcggaagtgg cgtcggaccg ggaggcggcc ctcctcgccg agcagcagaa ccagtccgac
     2401 cggatcgccg gtgcctcatt gagcggcgag acgggcgtcg accggatcct gatcgacctc
     2461 gcgcagcggg agagcatgac cgtctcggcg gatttcgcgg ccctgctgaa gcgcgaggcc
     2521 tcggcctatt tccccttccg ccccgaatat caccgcttcg cttctctggt ggtgctgaag
     2581 gcgccggacg tgccctcgat cctgttcgag acgggctacc tcaccaaccc gacggacacc
     2641 gcctacatcg catccccgga gggacggcag cagatcgcgc tggggatgaa gagcgcgatc
     2701 gagacccact tcgcgaggcg gttggtgagg gtggcggggc gctagtcttt cgccgccatc
     2761 gcggcctcca tgcgctcgcg cagcatgtcc tccaggcgcg actgccagcc cggtccggtg
     2821 gagcgcagcc accaaagaat atccggcgaa agccgaagtg aaaccagttc cttgcgggtg
     2881 ccctccggca atggaggcct tccgcgattt gtaagcgtgc cggttccctc acggaccacc
     2941 ttacccccgc gagtgaacgc cgcacgctcg aagaaggtgt cgtccgcctc gggaatatcg
     3001 tcgtcatccc agtccgcgac gaaaggtctt ccctcttcag aaacccgcga gatacctgaa
     3061 atatctttct tgctcattcc ggttggcctt tcgcatcgtc acgattcgaa cccgatcttc
     3121 ctcttggccg acccagacgc aggccacgac ttccccccga agcaacccgt aagaaaccca
     3181 tcgctgctca ccatagtcga agcggtcgtc ggcctcggtg aagcactcgc ccaggagcaa
     3241 ctcggccgcc tcagcgagat cgagtcccct ttcccgcagg actctcgcac gcttgaccgg
     3301 gtcgaattcg attttcacat aattattgta tatacgataa atcggaaaag tcaagctcgc
     3361 agccgcttca ccacgcttcg atcatcggct agagctacgc ggaatgtccg ctgaagccgc
     3421 tcccacccgc cttaccctct atcgcgacgc cggaagccgg ttcggcggcg ccgtgcggcg
     3481 cgcctggggc cgcagatggg tccggatcct cgcctatctg gccgcgatac cggttatcct
     3541 ctacttcctc ctctggctgc tgttcgcgcg caacctgccc tcggccgaga ccctgctcac
     3601 ctaccagccg ccgcttccca ccaacgttcg cggcatcgac ggcgagccgg tgcagacctt
     3661 cgcccgcgag cggcgcgtcg aattatcgtt cgacgaattc ccgcagaccc tggtcgacgc
     3721 cttcacctcg gccgaggaca agaccttctt cacccatggc gggatcgact atccgggcct
     3781 ggtaggcgcg gtcggcgact atatctccaa ggtcggcagc ggcgagcgcg cccgcggcgg
     3841 ctcgaccatc acccagcagg tcgccaagaa cctcctcctc ggcgacgaat attcggtcac
     3901 tcgaaagatc aaggaagcct tcctcgcccg ccggatcgag agcgtcctga ccaagcagca
     3961 gatcctcgcg ctctacctca accagatctt cctcggccgt aacgcctatg gagtccaggc
     4021 ggcggcccgg gcctatttcg acaaggacgt cgaggcgctc gagcttcacg aggtcgccta
     4081 tctcgccatc cttcccaagg cgccgagcaa ttacgatccg cagaggcgca cccagcgcgc
     4141 gctcgaccgg cgcaactggg tgctcgccga gatggagcgc aacggcagga tcacagccgc
     4201 ccagcgcgcc gccgcgcagg ccgagccgct cggcaccgtc cgcttcgcca ataccagcgt
     4261 tcgcaacgtc ggcggctatt tcgtcgagga agtgcggcgc cagctcatga cccgctgggg
     4321 cgagcagacc gagaaggggc ccaacggagt ctatacgggc gggctttggg tgcgcacgtc
     4381 gctcgacgtg cccaagcaga aggcggcgga gatggcgctt cgcgacggcc tgatgcgcta
     4441 cgaccggggc aagggctggc gcgatccggg cctcaaggtc gacgtttcag gcgactggcg
     4501 cggccagctg gcgcgggcgc cgttcggggt cggctatgag gattggcggg cggcggtggt
     4561 cctttcccgc gaggggcaga gcgcgacgat cggcttcacc gacggctcga ccggcgacct
     4621 ccccgcctac gccgcggcca tgccgaagcg cggcaccggc accgcggcct tcaacttcct
     4681 tcggccgggc atggtgatcg cggtcaagcg cgacggcggc atctggtccc ttcgatccat
     4741 tcccgaggtc aagggcggaa tggtcgtcga ggaagtggcg accgggcggg tgctcgccat
     4801 gcagggcggc ttcgacgcgc gcggcgacga tttcaaccga gccgtccagg cccagcgcca
     4861 gcccggctcg accttcaagc cgatcgtcta ttcggcggcg ctcgacaacg gcatgacccc
     4921 ggcctcgatc atcgtcgacg gcccgttctg cgccaatcag gggacgccga gccaatattg
     4981 cttccgcaac ttctcgggcg gccatgccgg cccgcagacg atgcgctggg gcctcgagca
     5041 atcgcgaaac ctgatgacgg tgcggacggc gagccagatc ggcatgaaca acgtcgtcga
     5101 gcgggcgcgg cggatgggcg tcggcgatta ccggccgttc ctgtcgatcg
//
LOCUS       DASWNU010000566         8674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_22176, whole genome shotgun sequence.
ACCESSION   DASWNU010000566 DASWNU010000000
VERSION     DASWNU010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22176"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..152
                     /locus_tag="VGB57_11185"
     tRNA            79..152
                     /locus_tag="VGB57_11185"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Cys,seq:gca)
     gene            276..404
                     /locus_tag="VGB57_11190"
     CDS             276..404
                     /locus_tag="VGB57_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131957.1"
                     /translation="MSKNAQKSARWVRPELKKLDAGSAEANATGINDGGTVGNARS"
     gene            491..2401
                     /locus_tag="VGB57_11195"
     CDS             491..2401
                     /locus_tag="VGB57_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY0131958.1"
                     /translation="MSAIAGVWSFGGEARAQALCGHLLDAQSAYGPHRRDWATHGDYS
                     AGRCLYRLLPEDEFDRQPVASEDGRFVLVADLRIDNRDELISSLSLGTADGSRLSDSD
                     LLLRGCLAWGRGILERMIGDFAFALWDGRERTLLLARDTAGERPLHYREGGGLVAYAT
                     MPLPLARLPGARFDVDEERLAGFVADLPPGGSRSFLAGVNILEPGHWALVTSAGIATE
                     SYWRPSRRPLHFARSEQYGEALREQLDAAVSRRLRRSGGLVGSQLSSGLDSSAVTTSA
                     ALALAAKGERLLAFTSAPREGFDGPVVAGRIGDESAIAAATARLHPNIDHHVVRPSGV
                     SALKLLESSHRLSGQPVGHVCNNVWWQGINRRAGEAGVRVMLTGEAGNFTISAGLGVD
                     TLGDLGRAGSWGRWWREARALRRRGQFSWPQMLNASFGSRLPEGLYARLRGRSPELPP
                     FVAPGWRDSMRRRLESAGWDVRPPRNSADRRWKYLRMFDPGNYRKRSLAEWGVEERDP
                     TNDRRLVEFCFSLPPEALLDGGVRRPALRRALAGRVPDAVLDQRLRGQQMPDWYEQIS
                     GDEVRAFARAQAESGLAAPFIDLDAANESARSWPTSGWGERPVIYHYRMYLLRTLSAA
                     SFVGSVRSGRLG"
     gene            2394..3311
                     /locus_tag="VGB57_11200"
     CDS             2394..3311
                     /locus_tag="VGB57_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131959.1"
                     /translation="MADLDYRYRLFGLTVQSEIELPELVADRGDGAADVRISIGTIAT
                     SPDAEAGLHVEGEDALLVIPAAGRFLSRAGIELLVEPAEGVSPRNLRLYLLGSAFGAI
                     LHQRRLLPLHANAVVVEGRAIGFMGHPGAGKSTLAAWFHDRGFDVLADDVCVVTRGAG
                     GQPLAHPGIPRLRLWREALEAGGRDASSYERSFDDMDKYTVPTDLDRMLPAVPLSHLY
                     VLEKAGEEDSPLVTRLEGSAAVEAMIANTYRGAYVRLMGQTRQHMLACVELARTVPVF
                     RARRRWGYDSFDVEGAALEAHARQLILES"
     gene            complement(3308..3760)
                     /locus_tag="VGB57_11205"
     CDS             complement(3308..3760)
                     /locus_tag="VGB57_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131960.1"
                     /translation="MLLLAVSTGPAAAQAPRPAAAVNAIPDQAGLARMVWSTMAAVDH
                     ANKTGNYNVLRGLGSPDFQASNGAPALSRIFASLREQGVNLSDTLSIEPSYEFPPALE
                     GGMLRLRGAFRMRPQAVQFDLLYQWNQGWMLQGIAVRTVPFSSLPPIR"
     gene            complement(3826..5721)
                     /locus_tag="VGB57_11210"
     CDS             complement(3826..5721)
                     /locus_tag="VGB57_11210"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131961.1"
                     /translation="MTKFTRIQLLAATAMSGAIFYAAPAAADPTAPCNNGTGLLTTEC
                     GTNSTTGASNFSTAVGQLATATGAESVALGADSLATGARSTALGTVSTALGDDSTAVG
                     NRASAGSAGGPAGNPTATAVGSGSVAGGRSSTAIGSGGVASRPTEALAGFSTAIGTGS
                     QVAAGATGATAIGDLNTITTNGSFSVALGAFNEVRAASGVAIGNGSVVAVGAVNGIAV
                     GSGADVPGEDGVAIGTLAFADADDAVALGTGAVANQAFNVALGSLSTATGVESVAVGG
                     DAQAVGAQSTAVGTVASANGALSSAFGNRASAAGAASTALGSLSRALGAESLAVGADS
                     LAEDANTTAVGVVATASGARATAVGHRSSASDEEAVAVGALSQATGFHSTAIGSQAIA
                     SGLGAQSFGWQSTASGARSTAVGRQSQATGAGSTALGDNARATGTNSTALGNLAVASG
                     ANSTALGNGATATFANSTAIGNGATTTAANQVTLGGTGTTVRFGGYTTAGVLVNDANG
                     VISSNTTLLPAVATLQTQTAANTAAIGTLNTQVTNLFDLNIANRRDIRKANEGVAMAL
                     AMETPTLPTGTSMGVAGGIGYYQNRLAGTASFAARVGTNAAFSAGVGLGFDSGEIGAR
                     AGFSTAW"
     gene            6112..8241
                     /locus_tag="VGB57_11215"
     CDS             6112..8241
                     /locus_tag="VGB57_11215"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY0131962.1"
                     /translation="MAGFELISGIEVDPRLRTFVEEEALPGTGIEPAAFWFGFSSLLR
                     ELTPENKRLLRRREDLQARIDERNAGLGGRPGDSAEEEEFLRDIGYLVDPPAPFEIGT
                     VNVDPEIALVAGPQLVVPVSNARYAINAANARWGSLYDALYGTDVLGDRPAAGGYDAE
                     RGGRVVAWGRAFLDELAPLTGGSHADVTRYGIEEGRLLTDCGTLRDPSLLAGWRGASP
                     LLRHHDLHVEIVVDPDHAIGRTDRAGVADILLESAVTAIMDCEDSVAAVDAEEKVAVY
                     RNWLGLMKGELSARFDKGGRGLERRLEPDRTYEAPGGGTLTLKGRALMFVRNVGHLMA
                     NPMIRIDGEEAQEGLVDAVVTALAALHDIQGNRSNSAAGSVYIVKPKMHGPEEAAFAG
                     RLFDRTEDLLGLPRHSVKIGVMDEERRTSVNLAAVIREVKDRVVFINTGFLDRTGDEI
                     HTAMKLGPVIRKAEMKGSEWLRTYEDNNVDVGLACGFAGRAQIGKGMWAMPDLMADML
                     AQKIAHPEAGASTAWVPSPTAAILHAIHYHRVDVAARQRELAARAPAALHGLLAVPVA
                     TERSWTPEAVREELDNNVQGILGYVVRWIDQGVGCSKVPDVHDVGLMEDRATCRISSQ
                     HVANWLMHGVVDHDEVEAALERMAAVVDRQNQGDPHYRPMAPDPAGSLAFRAARRLIL
                     EGAGQPSGYTEPLLHAHRLELKRRNPA"
     gene            complement(8150..>8674)
                     /locus_tag="VGB57_11220"
     CDS             complement(8150..>8674)
                     /locus_tag="VGB57_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131963.1"
                     /translation="KVMLYRKHLDVHSQLPPDSLSVSLNILAVSPATEFQEQYSFDTD
                     RSTIARVINASALETLVRLAGHIGGGKGAELIDRFAGHHPSERIRFAAWRARADLASD
                     PEARIAEYERAAAETSGLVSALAEATADRLRRARPWLEQAAEVRRDCGASAPADGRGE
                     GAPYSRTAARRPPG"
BASE COUNT         1318 a         2971 c         3014 g         1371 t
ORIGIN      
        1 tgcggacacg ggcgtccgcg cgcggacagc tcaaaaggtg gttgacttgg ccggtcaccg
       61 tggaacaagt gccgcggcgg cccgatggcg gagtggttac gcagaggact gcaaatcctt
      121 gcacgccggt tcgattccgg ctcgggcctc caccatgagt ggcactgaaa ccacaactca
      181 atctagaatt tctatccgcc aattagaggg ttggaattcc ttctccaccc ctgtaaacgg
      241 gccaacgaaa caacgaaaag cagggagcta gtcctttgtc caagaatgcg cagaagtccg
      301 cccgttgggt tcgtcccgag ctgaagaagc tcgacgccgg ctccgccgaa gccaacgcca
      361 ctggcatcaa cgacggcggc accgtcggca acgcgcgctc gtaagcgcgc cgtttccgag
      421 ggatagttta aggacgtccg cctttcggcg gacgtctttt tcgtgcgtcc gctccctgcg
      481 ggtctgaacc ttgtccgcca tcgcaggtgt ctggagtttc ggcggggagg ctcgcgccca
      541 ggcgctttgc ggccatttgc tcgatgccca atccgcctac ggtccgcatc gccgcgattg
      601 ggccacccat ggcgactatt cggcgggacg gtgcctctac cggctgctgc ccgaagatga
      661 gttcgaccgt cagccggtag cgagcgagga cgggcgcttc gtgctcgtcg cggaccttcg
      721 gatcgacaat cgggacgagc ttatctccag tctctccctc ggcaccgcgg acgggtcgcg
      781 gctcagcgac tcggacctgc tcttgcgcgg ctgcctcgcc tggggccggg gcatattgga
      841 gcgcatgatc ggcgacttcg ccttcgccct ttgggacggc cgcgagcgca ccctgcttct
      901 cgcccgcgac acggccggcg agaggccgct ccattatcgc gaaggcggcg gtcttgtcgc
      961 ttatgcgacc atgcccctcc ccctcgcccg ccttcccggc gcccgcttcg acgtcgacga
     1021 ggagcggctt gccggattcg tcgccgacct tcctcccggc ggcagccgca gcttcctggc
     1081 cggcgtcaat attctggagc ccggtcattg ggcgctcgtc accagcgccg gaatcgccac
     1141 ggaaagttat tggcggccca gtcgccggcc gctccatttc gcccgaagcg agcaatatgg
     1201 cgaggccctt cgggagcagc tcgacgccgc cgtctcccgc cggctccgac gctcgggcgg
     1261 cctcgtcggc tcccagctca gctcgggcct ggacagcagc gcggtgacga cctcggccgc
     1321 tctcgccctc gccgccaaag gcgagcgcct gctcgccttc acctcggcgc cgcgcgaggg
     1381 cttcgacggc ccggtggtcg caggccgaat cggcgacgaa tccgcgatcg ccgccgccac
     1441 ggcgaggctt catcccaata tcgaccatca tgtggtgcgg ccgtccggag tctcggcgct
     1501 gaagctgctc gaaagcagcc accgcctctc gggccagccg gtcggccacg tctgcaacaa
     1561 tgtctggtgg cagggcatca accgccgcgc cggcgaagcg ggcgttcggg tgatgctgac
     1621 cggcgaggct ggaaatttca cgatcagcgc cggcctgggc gtcgacacgc tgggggacct
     1681 cggccgcgcc ggctcgtggg ggcgctggtg gcgggaggcg cgggcattga ggcgcagggg
     1741 ccagttcagc tggccgcaga tgctcaacgc gtcgttcggg tcgcggctgc ccgaaggcct
     1801 ctatgcccgg ctgcgcggcc gaagcccgga gctgccgccc ttcgtcgcgc ccggctggcg
     1861 cgactcgatg cgacggcggc tcgaaagcgc gggctgggac gttcggcctc cccggaactc
     1921 cgcggaccgc cgctggaagt acctgcgcat gttcgatccc ggcaactacc ggaagcggag
     1981 cctcgccgaa tggggcgtcg aggagcgcga tccgaccaac gaccggcggc tggtcgaatt
     2041 ctgtttctcg cttccgcccg aggcgctgct cgacggcggc gtgcgccggc cggcgctccg
     2101 gcgcgctctg gccggacggg tcccggacgc cgtgctcgac cagcggcttc gcggccagca
     2161 gatgcccgac tggtacgagc agataagcgg ggacgaagtc cgggccttcg cccgggcgca
     2221 ggcggaatcg ggtctcgccg ctcccttcat cgatctcgat gcggcgaacg aatcggctcg
     2281 aagctggcca acctccggct ggggggagcg gcctgtaatc taccattacc ggatgtacct
     2341 gctgcggacg ctctccgcgg cgagcttcgt cgggtcggtg aggagcggcc gccttggctg
     2401 atctcgacta tcgctaccgc ctgttcgggc tcacggtcca gtcggagata gagttgccgg
     2461 agctcgtcgc cgatcggggt gacggagcgg cggacgtccg gatctcgatc gggacgatcg
     2521 ccacgtctcc cgatgcggag gcggggctgc atgtcgaggg tgaagacgcc cttctcgtca
     2581 ttcccgcggc cggccgcttc ctctcccgtg ccgggatcga gctgctcgtc gagcccgccg
     2641 agggggtctc tccgcgcaac ctgcgcctct atctgctcgg ctccgccttc ggggcgatcc
     2701 tgcaccagcg ccgcctcctt cctctgcacg ccaacgcggt ggtggtggag ggccgggcca
     2761 tcggcttcat gggccatccc ggcgcgggca agtcgacgct cgcggcctgg ttccacgacc
     2821 gcggcttcga cgttctggcc gacgacgtct gcgtcgtcac ccggggcgcg ggcgggcagc
     2881 cgctggccca tcccggcatt ccccggcttc gcttgtggcg cgaggccctg gaagcgggcg
     2941 gccgcgacgc ctcgtcctac gagcgttcgt tcgacgacat ggacaaatat acggtgccca
     3001 ccgatctcga tagaatgctt cccgcggtgc cgctcagcca cctctatgtg ctggagaaag
     3061 ccggggagga ggactcgcct ctggtcaccc ggctcgaagg ctcggcggcg gtggaagcga
     3121 tgatcgccaa cacctatcgc ggcgcctacg tccggctgat ggggcagacc cggcagcaca
     3181 tgctcgcctg cgtcgagctt gcccggacgg tacccgtgtt cagggctcgg cggcgctggg
     3241 gctatgacag cttcgatgtg gaaggggccg cgctcgaagc gcatgcgcgg cagcttatcc
     3301 tcgagagcta gcggatcggc ggaaggctcg agaagggcac cgtccttacg gcgatgcctt
     3361 gcagcatcca gccctggttc cactgataaa gtaggtcgaa ctgtaccgcc tgggggcgca
     3421 ttcgaaaggc gccgcgcagc cggagcatcc cgccctccag cgccggcggg aactcgtagg
     3481 agggctcgat cgacaatgtg tcgctgaggt tcaccccctg ctcccgaagg cttgcgaaaa
     3541 ttcgggagag agccggcgcg ccgttgcttg cctggaaatc cggggatccg aggccgcgaa
     3601 gcacgttgta attgccggtc ttgttggcgt gatcgaccgc cgccatcgtg ctccacacca
     3661 tccgggcgag gccggcctga tcgggaatgg cattgaccgc tgccgcgggc cttggcgcct
     3721 gcgccgccgc cggtcccgtc gagaccgcca gcagaagcac ggccaacagc aaacgggccg
     3781 gggcgtgaag ccccggcccg ctcgtttggc cttgttcagg cctgcttacc acgcggtcga
     3841 gaagccggcg cgagcgccga tctcgccgct gtcgaagccc aggccgacac cggccgagaa
     3901 ggcggcgttg gtgccgacgc gagcggcgaa ggacgccgtg cccgcgaggc ggttctggta
     3961 gtagccgata ccgccggcga cacccatgct ggtgccggtc ggaagggtcg gagtctccat
     4021 cgccagcgcc atggcgacgc cctcgttggc cttgcggata tcgcgacggt tggcgatgtt
     4081 cagatcgaac aggttggtca cctgcgtgtt gagcgtcccg atcgcggcgg tgttggccgc
     4141 ggtctgggtc tgcagggtgg cgacggccgg cagcagggtg gtgttgctgc tgatgacgcc
     4201 gttcgcatcg ttcaccagga cgcccgccgt ggtgtagccg ccgaagcgga ccgtggtgcc
     4261 ggtgccgccg agggtgacct ggttggccgc cgtcgtggtg gcgccgttgc cgatcgccgt
     4321 cgaattggcg aaggtggcgg tggcgccgtt gccgagcgcc gtcgaattgg cgccgctcgc
     4381 cacggcgagg ttgccgaggg cggtcgaatt ggtccccgtc gcacgggcat tgtcgccgag
     4441 agccgtcgaa ccggcaccgg tcgcctgcga ctggcggcca acggccgtcg agcgggcgcc
     4501 cgaagcggtc gactgccagc cgaacgactg cgcaccgaga cccgaagcga tggcctggct
     4561 accgatcgcc gtcgagtgga agccggtggc ctgcgacagc gcgcctaccg cgaccgcctc
     4621 ttcgtcggac gccgaagacc gatggccgac ggccgtggcg cgagcgccgc tggcggtcgc
     4681 gacgacgccg acggcggtgg tgttcgcgtc ctcagcgagc gagtccgcgc cgacggcgag
     4741 ggactcggcg ccgagcgcgc gggagagcga gccgagagca gtgctcgcgg cgccggcggc
     4801 gctggcgcgg ttaccgaagg cgctggagag cgccccgttg gcgctggcga cggtgccgac
     4861 cgcggtcgac tgggcgccga ctgcctgcgc atcgccgccg acggcgaccg attcgacgcc
     4921 ggtggcggtg ctgaggctgc cgagagcgac gttgaacgcc tggttcgcta ccgcgccggt
     4981 gcccagggca acggcgtcgt cggcgtcggc gaaggcgagc gtgccgatgg cgacaccgtc
     5041 ctcgccgggc acgtcggcgc ccgaaccgac cgcgataccg ttgaccgctc cgacggcgac
     5101 gaccgaaccg ttgccgatgg cgacgccgct cgcggccctg acttcgttga acgcgccgag
     5161 cgcgacactg aacgagccgt tggtggtgat cgtgttcaga tcgccgatgg cggtcgcgcc
     5221 ggtagcgccg gcggcgacct ggctaccggt gccgatggcg gtgctgaagc ccgcaagcgc
     5281 ttcggtagga cggctggcga cgccaccgct gccgatcgcg gtcgaggaac ggccgccggc
     5341 gacggagccg gaaccaaccg cagtagcggt ggggttgcca gcagggccac cagcggaacc
     5401 agccgacgcg cggttgccga cggcagtcga atcgtcgccg agggcggtcg agaccgtgcc
     5461 gagggccgtc gaacgggccc cggtagccag ggagtcggcg cccagcgcga cgctctccgc
     5521 gccggtggcg gtggcgagct ggccgacggc ggtgctgaaa ttggaagcgc cggtcgtgga
     5581 attggtgccg cactcggtcg tcagcaagcc ggtgccgtta ttgcacggcg ccgtcggatc
     5641 ggcggcggcc ggagccgcgt agaagatggc ccccgacatg gcggtcgcgg ccaagagctg
     5701 aatcctggta aatttggtca tgaacttcct ccctgtatgg atttgaaact tatggtcggt
     5761 ccgcgatggg actgccctga aaacaggttg aaccctgcac gctccctctt cggcagccgc
     5821 acactcgctg gctggcgccg ttttcggaga tgcacctttg gcttgcaagc ctgattcgtc
     5881 gcgcgatggc gccagaatca actattttgg aggatttcga cctccttccc cacttttccg
     5941 cccgatgcgc gtcctggccg ccacactcgg gaaacaataa caggtccgga tcgaggcccc
     6001 gggaaccttt ggccaccctc aagaggagcg catgccggct cgccgacagg catggataaa
     6061 cgcccatcca gacgctaggc ggaccccgcc acgaagaaaa ggggagcgga tttggccgga
     6121 ttcgagctga tctccgggat cgaggtggat ccgcggctga ggaccttcgt cgaagaggaa
     6181 gcgcttcccg gcaccgggat cgaacccgcc gccttctggt tcggcttctc ttcgctgctg
     6241 cgcgagctta cgcccgagaa caagcgcctc ctgcgccgcc gcgaggatct gcaggcccgg
     6301 atcgacgagc gcaacgccgg cctcggcggc cgcccgggcg actcggccga ggaagaggag
     6361 ttccttcgcg acatcggcta tctcgtcgac ccgcccgccc ccttcgaaat cggcaccgtg
     6421 aacgtcgacc cggagatcgc gcttgtcgcg gggccgcagc tggtcgtgcc ggtgagcaac
     6481 gcccgttacg cgatcaacgc cgccaatgcc cgctggggca gcctctacga cgccctctac
     6541 gggaccgacg ttctcggcga ccggccggcg gccggcggtt acgatgccga gcggggggga
     6601 cgggtggtcg cctgggggcg ggcctttctc gatgagctgg cgcccctgac cggcggcagc
     6661 catgccgatg tcacccgcta cggcatcgaa gaggggcgac tcctcaccga ttgcggcact
     6721 ctgcgcgatc cctcgctgct cgccgggtgg cggggcgcct cgccgctgct gcgccatcac
     6781 gatcttcacg tcgagatcgt cgtcgatccc gaccatgcga tcggccgcac cgacagagcc
     6841 ggcgtggccg acatcctgct cgagagcgcg gtcaccgcga tcatggactg cgaggattcg
     6901 gtcgccgccg tcgacgccga ggagaaagta gccgtctacc gcaactggct cggcctgatg
     6961 aaaggcgagt tgagcgcccg cttcgacaaa ggcggccgcg gtctggagcg gaggctcgag
     7021 ccggaccgca cctacgaggc gcccggcggc ggcactctga cgctcaaggg ccgcgccctg
     7081 atgttcgttc gcaacgtcgg ccatctgatg gccaacccga tgatccggat cgacggcgag
     7141 gaggctcagg agggcctggt cgacgccgtc gtcaccgcgc tcgccgcgct ccacgatatc
     7201 cagggaaacc gctccaacag cgcggccggc tccgtctata tcgtcaagcc caagatgcac
     7261 gggcccgagg aagccgcctt cgccgggcgc ctcttcgacc gcaccgagga cctgctcggc
     7321 cttccccgcc acagcgtcaa gataggcgtg atggacgagg agcgccggac cagcgtcaac
     7381 ctcgcggccg tgatccgcga ggtgaaggac cgggtggtgt tcatcaacac cggcttcctc
     7441 gaccgcaccg gcgacgagat ccataccgcg atgaagctgg gcccggtgat ccgcaaggcc
     7501 gagatgaagg gctccgagtg gcttcggacc tacgaggaca ataatgtcga cgtcggcctg
     7561 gcctgcggtt ttgccggccg cgcccagatc ggcaagggca tgtgggcgat gccggacctg
     7621 atggccgaca tgctcgccca gaagatcgct caccctgagg ccggggcaag caccgcctgg
     7681 gtcccctccc ccaccgccgc catcctccac gcaatccact atcaccgggt ggacgtggcg
     7741 gcgcggcagc gcgagctcgc cgcccgagcg cccgccgcgc tgcacgggct tctggcggtc
     7801 ccggtggcga cggagcgcag ctggactccc gaagccgtcc gcgaggagct cgacaacaac
     7861 gtgcagggca tactcggcta cgtcgtgcgc tggatcgacc agggcgtcgg ctgctcgaag
     7921 gtgccggacg tccacgacgt cgggctcatg gaagaccgcg cgacctgccg aatctcaagc
     7981 cagcatgtcg ccaactggct gatgcacggc gtggtcgacc acgacgaggt ggaagccgcg
     8041 ctggagcgga tggcggccgt cgtcgatcgc cagaaccaag gcgatcctca ttatcgcccg
     8101 atggctccgg atccggccgg gagcctcgcc tttcgagccg cccgccgcct tatcctggag
     8161 ggcgccgggc agccgtccgg ctatacggag ccccttctcc acgcccatcg gctggagctg
     8221 aagcgccgca atcccgcctg acctccgccg cctgctccag ccagggcctc gcccggcgaa
     8281 gccggtccgc ggtggcctcg gcgagggcac tgaccaggcc ggacgtctcc gccgccgccc
     8341 gctcatattc ggcgatcctg gcttcgggat cggaggcgag gtcggcccgg gcccgccagg
     8401 cggcgaaacg aatgcgctcg ctgggatgat ggccggcgaa gcgatcgatc agctccgccc
     8461 ctttcccgcc gccgatatgc cccgccagcc tcaccagggt ctccaaggcg gaggcgttga
     8521 tcacccgcgc gatcgtcgag cggtcggtgt cgaagctgta ttgctcctgg aattcggtcg
     8581 ccggcgaaac cgcgaggatg ttgagcgata ccgaaaggct gtccggtgga agctggctgt
     8641 gcacgtcgag gtgcttgcgg tacagcatca cctt
//
LOCUS       DASWNU010000567         5237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_307090, whole genome shotgun sequence.
ACCESSION   DASWNU010000567 DASWNU010000000
VERSION     DASWNU010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307090"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2069
                     /locus_tag="VGB57_11225"
     CDS             <1..2069
                     /locus_tag="VGB57_11225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0131964.1"
                     /translation="ALAAYDAGATGAGVKVAVVDTGINPNLPEFTGRIDPASQDVAAD
                     RGIVDHQGHGSMVSGVIAANRDGIYMQGVAYEATILSLNVYDPAGCKPGNDCFLDAAI
                     DDAIDLAVANGAKIINMSFGDEEGMPEETFAAVQRAVNAGIIVIMSAGNLPTPDPNSF
                     AVKNVQQGGATGLFIIAGSIDANKSISSFSSRAGTGVSAAHYLTALGRDNATVNHLGA
                     HVAVDGTSFSAPTIAGAAALLAGAFPNLTGAQIVQLLLTTADDAGAAGTDPIYGRGIL
                     NIAKAFQPQGATVLAGSMAAISLSDNGTVSGPMGDAAPGQSGGAIILDGYSRAYALDL
                     ARTLGRVSQEQPLAQGVSGGSFRTAAASSGRYSVAVTIRRNADGGPGVEMEQLHLDHE
                     DGRRARAVAGMALSRLTPKTALAFGFSQSGHMLQQQLAGRWDNEFLVARDPASRAGFH
                     QEADTSLGLRHDFGPLALTVTSERGKVHMPGLRHRLDRPSYTLDTLTFDRKIGPARFS
                     LGASRLTEEATILGARFSSIFSNGGSRTLFADGTAGLDLGAGFEAYASYRRGWTSMPG
                     TGSLVEKGRLRTEAFAFDLSRTGMFAAGDKLAFRVMQPLRVASGGFDLDMPVGYDYAT
                     GAARFERRFFNLAPTGREIDYEVAYGLGLLGGRLDFNAFLRTDPGHIETMKTDMGTAI
                     RFTLGR"
     gene            complement(2204..2620)
                     /locus_tag="VGB57_11230"
     CDS             complement(2204..2620)
                     /locus_tag="VGB57_11230"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HEY0131965.1"
                     /translation="MKRIATTAMAAAIMLAGTAGAQQPAQRPQAQRPPAQAQQRAPAQ
                     AQRVPTRVTARTLTALCSQDRSGCLTYVLGAADAFSSALVAAGRPQGFCFPRGTTNDQ
                     IAQTAVRYLRANPQEGGNNAGLVILAGLTTAYPCGY"
     gene            2740..3177
                     /locus_tag="VGB57_11235"
     CDS             2740..3177
                     /locus_tag="VGB57_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0131966.1"
                     /translation="MNSPILAPVVALVAWTLLIMAWMVVSRFAAMKRKGISLKGRIGG
                     RGGAALEGVVEDEVMWKAHNYMHLVEQPTLFYAVALALAVGHAGGGYSAILAWSYVGL
                     RIAHSLVQTTINVITYRFILWTLSSLALAGLTVQAIRMVCGHF"
     gene            complement(3351..4526)
                     /locus_tag="VGB57_11240"
     CDS             complement(3351..4526)
                     /locus_tag="VGB57_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY0131967.1"
                     /translation="MSDTIVRLAGRGDGVGESGRFHPLTAPGDRVGADGRIEAGPHRQ
                     VPPCRHFPECGGCQLQHVVDEAYAGFLRDRIASALAAQGVPSPDILAAHISPPNSRRR
                     ASLRAERGGALGFNAEASHRVVDMRECHILRPELFALVQPLRALLRGLKLSATAGVRM
                     TLTDQGVDLLLEKVEAEGLAAAEALGAFARQHGLARLALDDGYGAETHWEPEPVTVTL
                     GGVPVPLPHGAFLQATAEGEAALVEAVRRSVGDARLVADLFAGLGTFALALEGRILAA
                     EAARDAALALKSAAGRAQRQVFVDHRDLYRRPLDPAELNRFEAVVLDPPRAGAKEQAA
                     SLARAEVPRIAYVSCNPSTFARDSAALIEGGYRFDWIAPVGQFRWSTHVELVGAFSR"
     gene            complement(4523..>5237)
                     /locus_tag="VGB57_11245"
     CDS             complement(4523..>5237)
                     /locus_tag="VGB57_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0131968.1"
                     /translation="SRGYVAVVSGRMGGASALAAEAALRGGAGYVRLHADDFLGRTAR
                     AVVRSGDLDALFDRRVGAVIAGPGLGLGDLEAGILNLVITTAIPAVLDADALTLIADT
                     GLDWLHRAPRTPILTPHAGEFARLFPDLLGSKVEQARTAAASARSVIVYKGPDTVVAA
                     PDGRAAIARPAPAWLASAGTGDVLAGVVAATRASGLDAFEAACAGVWLHGRAAELAGP
                     GLIADELLDHLASALAECL"
BASE COUNT          822 a         1783 c         1843 g          789 t
ORIGIN      
        1 acgcgctcgc cgcctacgat gccggcgcga ccggcgccgg cgtcaaggtc gcggtggtcg
       61 acaccggcat caaccccaac ctccccgaat tcaccgggcg gatcgacccg gcgagtcagg
      121 acgtcgcggc cgaccgtggc atcgtcgacc atcaggggca tggcagcatg gtctccggag
      181 tcatcgccgc gaaccgggac ggcatctaca tgcagggcgt cgcctatgag gcgacgatcc
      241 tgtcgctgaa cgtctacgac ccggcgggct gcaagccggg caacgactgt ttcctcgacg
      301 ccgcgatcga cgatgcgatc gaccttgccg tggccaatgg cgccaagatc atcaacatgt
      361 ccttcggcga cgaggaaggg atgcccgagg agaccttcgc cgccgtccag cgcgcggtca
      421 atgcaggcat catcgtcatc atgtccgccg gcaacctgcc gacgccggac ccgaactcct
      481 tcgcggtcaa gaatgtccag caaggggggg ccaccggcct gttcatcatt gccgggtcga
      541 tcgacgccaa caaaagcatc agcagctttt cgagccgcgc cggcaccggc gtcagcgccg
      601 ctcactatct gacggcgctc ggccgcgaca atgcgaccgt caaccacctc ggcgcgcacg
      661 tcgccgtcga cggcacgagc ttttcggcgc ccaccatcgc cggcgccgcg gccctgctcg
      721 ccggggcgtt cccgaacctg accggagccc agatcgtcca gctgttgctg acgaccgccg
      781 acgatgccgg agcggccggc accgatccga tctacggccg cggtattctc aacatcgcca
      841 aggccttcca gccgcagggc gcgacggtgc tggccgggag catggcggcc atttcgctga
      901 gcgacaacgg gaccgtctcc ggtccgatgg gcgatgccgc gcccggccaa agcggaggcg
      961 cgatcatcct ggacggctat tcgcgcgcct atgcgctcga tctcgccagg acgctcgggc
     1021 gcgtgtcgca ggagcagccg ctcgcgcagg gcgtgagcgg gggaagtttc cgcaccgccg
     1081 ccgcgtcctc tggaagatat tcggtcgcgg tcacgatccg ccggaatgcg gacggcgggc
     1141 cgggcgtgga gatggagcag ttgcacctcg accatgagga tggccgaagg gccagggcgg
     1201 tcgccgggat ggcgctcagc cgcctgactc cgaagacggc cctcgccttc ggcttttccc
     1261 aaagcggcca tatgctccag cagcagctcg ccgggcgatg ggacaatgaa ttcctcgtcg
     1321 cccgcgatcc ggcgagccgc gccggcttcc atcaggaagc cgacaccagc cttggccttc
     1381 gccacgattt cggaccgctc gcactgaccg tgaccagcga gcgcggcaag gtgcacatgc
     1441 ccgggctccg gcatcggctc gaccggccgt cctacactct ggacaccctg acgttcgaca
     1501 ggaagatcgg cccggcgagg ttcagcctgg gtgcttcgcg actgactgag gaagccacga
     1561 tattgggcgc ccgcttctcc tcgatcttct cgaacggcgg ctcgcggacc ctgttcgccg
     1621 acggcacggc cggccttgat ctcggcgccg gcttcgaggc ctatgccagc tatcgccgcg
     1681 ggtggacgtc catgcccgga acgggctcgc tggtggagaa gggccgcctc cggacggagg
     1741 ccttcgcctt cgatctgtcg aggaccggca tgttcgcggc cggagacaag ctcgccttcc
     1801 gggtgatgca gccgcttcgg gtcgcgagcg gcgggttcga cctcgatatg ccggtcggct
     1861 acgactatgc gacgggagcg gcgcgtttcg agcggcgctt cttcaacctt gcgccgacgg
     1921 ggcgggaaat cgactatgag gtcgcctacg gcctcggcct cctcggcggc cgcctcgatt
     1981 tcaacgcctt cctccgtacc gatcccggcc atatcgagac gatgaagacg gacatgggca
     2041 cggcaatcag gttcaccctc ggaagatagg ttccgcctgg tggctggacg gagcgaaggt
     2101 gttcgaccga tcggcctcac ctcatcattg cgaggagcga agcgacgggc ctcggaggct
     2161 tagctggatt gcttcgcttc gctcgcaatg acggtgttgg aactcaatag ccgcaggggt
     2221 aggcggtcgt gaggcccgcc aggataacga ggccggcatt gttgccgcct tcctgcggat
     2281 tggcacgcaa atagcgcacg gccgtctggg cgatctggtc gttggtggtt ccccttggaa
     2341 agcagaagcc ctgcggccgg ccggccgcga ccagagcgct ggaaaaggcg tcggcggcgc
     2401 cgagcacata ggtgaggcag ccgctgcggt cctggctgca aagcgcggtg agggtgcggg
     2461 cggtgactcg ggtcggcact cgctgcgcct gggccggggc gcgctgctgg gcctgggccg
     2521 gcggacgctg ggcttgcgga cgctgcgccg gctgctgggc cccggcggtc ccggccagca
     2581 tgattgccgc cgccattgcc gtcgtcgcga tccgcttcat ctcttcactc ctccactctg
     2641 cggcaccctg tttagcaggc tttttcagcc actaaagggc gctttgactc cggcgtccac
     2701 ggcggccact ctggcgcctc aaaaggggga aacgaagcca tgaacagtcc cattctcgcg
     2761 cccgtcgtcg cgctcgtcgc ctggaccttg ctgatcatgg cctggatggt cgtgagccgc
     2821 ttcgcggcga tgaagcgcaa gggcatttcc ttgaagggcc ggatcggcgg ccgcggcggc
     2881 gccgcgctcg aaggcgtggt cgaggacgag gtgatgtgga aggcgcacaa ttacatgcac
     2941 ctggtggagc agccgaccct gttctacgcg gtcgccctgg cgctcgcggt gggacatgcc
     3001 ggcggcggct acagcgcgat cctcgcctgg tcctatgtcg gcctgaggat cgcgcacagc
     3061 ctggtgcaga cgacgatcaa cgtcatcacc tatcgcttca tcctgtggac gctgtcgtcg
     3121 ctggccctgg ccgggctcac ggtgcaggcg atccggatgg tgtgcggcca tttctaggcc
     3181 gtagcggcac ccggcgcggt accgactgca acatccggat cggatccgtc tgaaggagat
     3241 tccaaggttg atgttccgat attgttcttc atgccgagtc gcgtatcggc gggctctttg
     3301 acattgtcgg gacgatttcg agtcggggct aaagacgccg aggcgcggtc tcagcggctg
     3361 aacgccccga ccagctcgac gtgggtcgac cagcggaact ggccgaccgg cgcgatccag
     3421 tcgaagcgat aaccgccttc gatcagggcc gcggagtcgc gggcgaaggt cgaggggttg
     3481 caggagacgt aggcgatgcg cggcacctcc gcgcgggcga gcgacgccgc ctgctccttc
     3541 gccccggccc ggggcggatc gagcacgacg gcctcgaatc ggttgagctc cgccggatcc
     3601 agcggacggc ggtagaggtc gcgatggtcg acgaagactt gccgctgggc ccgtccggcc
     3661 gcggacttca gcgcgagggc ggcgtcgcgg gcggcctcgg cggcgaggat cctgccctcg
     3721 agcgccaggg cgaaggtgcc gaggccggcg aacaggtcgg cgacgaggcg cgcatcgccg
     3781 accgaccgcc gcaccgcctc gaccagcgcc gcctcgccct ccgccgtggc ctggaggaag
     3841 gcgccgtgcg gaagcggcac ggggactccg cccagagtga ctgtcaccgg ctccggctcc
     3901 caatgggtct cggcgccgta gccgtcgtcc agcgccagcc gggcgaggcc gtgctgccgg
     3961 gcgaaggcgc ccagcgcctc ggccgcggca agcccctccg cctcgacctt ctcgagcagc
     4021 aggtcgacgc cctgatcggt cagggtcatc cgcactccgg ccgtggccga gagcttcagg
     4081 cctcgaagca gagcgcgaag cggctggacg agcgcgaaaa gctcgggccg aaggatgtgg
     4141 cattcgcgca tgtcgacgac ccggtggctc gcctcggcgt tgaagccgag cgctccgccc
     4201 ctttcggccc gaagcgaggc ccggcggcgg ctgttgggcg gagagatgtg cgcggccagg
     4261 atgtccggcg aagggacccc ttgggcggcc aaagcggagg cgatgcggtc gcgcaggaag
     4321 ccggcataag cctcgtcgac gacatgctgc agctggcagc cgccgcattc ggggaaatgc
     4381 cggcagggcg gcacctgccg atgcggtccc gcctcgatgc ggccgtcggc gccgacccgg
     4441 tcgccggggg cggtgagcgg gtggaagcgc ccgctctcgc cgacaccgtc gccccggccg
     4501 gcgagcctga cgatcgtgtc gctcacaggc actcggccaa agcggaggcc agatgatcga
     4561 ggagttcgtc ggcgatcaag cccgggccgg cgagctcggc cgcccggccg tgcagccaga
     4621 cgccggcaca ggcggcttcg aacgcgtcga ggcctgacgc gcgggtcgct gcgacgaccc
     4681 ccgccagaac gtcgccggtg ccggcgctcg ccagccaggc cggggccggc cgggcaatcg
     4741 ccgctcgccc gtcaggcgct gcgacgaccg tgtcgggtcc cttgtagacg atcaccgatc
     4801 gagcggaggc ggcggcggtc cgggcctgct cgaccttgct tccgagcaaa tccggaaaca
     4861 gcctcgcaaa ctcccccgca tggggagtga ggatcggagt cctcggcgcc cggtggagcc
     4921 agtccagtcc ggtatcggcg atgagggtca gggcgtcggc gtccagaacc gcagggatgg
     4981 cggtggtgat gacgaggttg aggatcccgg cttcgaggtc gccgagtccc agccccggtc
     5041 cagcgatcac cgctccgacc cggcggtcga agagagcgtc gaggtcaccg ctccgaacga
     5101 cggcgcgagc ggtcctgccg agaaaatcgt cggcgtggag ccggacatag ccggcaccgc
     5161 cccgaagcgc ggcctcggcg gcaagggccg aggccccgcc catacgcccg gaaaccacgg
     5221 cgacgtagcc gcgactg
//
LOCUS       DASWNU010000568         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_99846, whole genome shotgun sequence.
ACCESSION   DASWNU010000568 DASWNU010000000
VERSION     DASWNU010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99846"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VGB57_11250"
     CDS             complement(<1..456)
                     /locus_tag="VGB57_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY0131969.1"
                     /translation="MSGEALGPVQRWMHEALVFPRRVDGERRDALLRASPRLSAAAGL
                     AIYQRGYFLRIASCMREQFPALCHALGEPLFDDFVADYIKDRPPESHTLYDLGRRFPA
                     WLDENRPDRGRPEAERESWIDFMIDLARFERQVFAMFDAPGHEGRPFAGA"
     gene            complement(453..1403)
                     /locus_tag="VGB57_11255"
     CDS             complement(453..1403)
                     /locus_tag="VGB57_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY0131970.1"
                     /translation="MAAAEDPVADLPRLGLGLGLRSVHFEEILTRRPKVDWFEAISEN
                     FMDSGGRPRAVIREVAEHYPVVLHGVSLSIGSADPLDLDYLDRLKRLADEVKPAWISD
                     HLCWTGVMGLNSHDLLPMPLTEESLTHVAARVRRAQDFLDRPLILENPSSYVRFAAST
                     MEEPEFLRRLAGETGCGLLLDVNNVFVSCFNAGTDPVAYIAAFPCEHVVQMHLAGHQH
                     EGTHVVDTHDRTVRPEVWELFRLAWSLTGGASTLLEWDGDIPALADCQAELLKANDYM
                     AGRFDPPPAGPVEAARAGSLSNPVDFLVPRVMDSNLLEEA"
     gene            complement(1390..2058)
                     /locus_tag="VGB57_11260"
     CDS             complement(1390..2058)
                     /locus_tag="VGB57_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131971.1"
                     /translation="MASCSTPPTPATPAPGVPVPASSPYVANPPVFPTSIGDGPGGMP
                     SHACMGLNSCAASDRFGTAGAPGLGPNACAGQGYCSTATDHTCHVQNDCRDQGGCGLY
                     GTGEEMNRPGENDCRSLGSCATPINAERFSTNGPNQGQSVWLRARAVFEEKWAAKRED
                     LLEAQAAGHIPADQPLPETPGSPPPVFAATGPTYLWISNVNSARSNMIACGASGLSGA
                     GGCS"
     gene            complement(2102..3682)
                     /locus_tag="VGB57_11265"
     CDS             complement(2102..3682)
                     /locus_tag="VGB57_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0131972.1"
                     /translation="MNEQPSSIAAGQPDPITPLTPVEEAFVVWLDLTSSREEVLAELK
                     ANLQTAVEIELATIPIYLYTYYSLHRNSESGETISDAQLYANKAGGLIMSVAVEEMLH
                     MSLSSNILWSMGVPPRLYGRAPSSYPTGLPYHNPKGPAGPDGSTPVLIPLERLSFEQL
                     WHFLQIEYPEQWDALPQDRNWDTIGQFYSYIRCLLRTRFVTDADFQQGAAASAIQPYN
                     YSPNNVDTVYPGSGFDPWKPAPPVPAPSWADGKPGAAEVAVFPDRADSHSGPTELISI
                     ASRADAAAAIDTICDQGEGCPVPDVGPSPDDDPTKDEASHYMKFLTLQAQFADYAGCT
                     EVLPPEPEPPQPITPSVTDDALLAAGVLIDLPDSPRSIDYPFQYRAIADFSSALFQYM
                     LIMCETIYRVPPANQKLFFNEGLHRSMIWVLDKYIQTIRKIEIPSGPHACRMMGPLFE
                     NVNLGAPEDSFAGLTEYGNAAIAAATALSQGIDPNGNLASALGNVTYYVGVALTQTSS
                     DGAPMHLPDVGPYWKAAS"
     gene            complement(3679..>4629)
                     /locus_tag="VGB57_11270"
     CDS             complement(3679..>4629)
                     /locus_tag="VGB57_11270"
                     /inference="COORDINATES: protein motif:HMM:NF040572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0131973.1"
                     /translation="VLGALQLLVGTWTNQPYPNGKGGPDSPYSYNLMVLPQSDSVTTP
                     EGFILKNMQYYEEITFSPIHGNAANRGGTGVQVCNPLFYEQRIYISEASGTPATNTLV
                     HAENGSWLVLSSSVQLEGPYSTGPEVPGSTPPTGFPTLVKQISVPHGNSVLALGEAPP
                     FPLQPQSGTPSIPDLTGTVIPAGVGPAPYTTPGVGNPNTAYTANPNAPLAEALGQANV
                     VSYIKFQVDTAFANSKGMVTNIGYEERHANVMRYSATYWLEQLEGSDGYDQLQYSQTI
                     TLEIPLHGTVYKFPHVTSNTLTRVEGLSPSTAGLGTEAQS"
BASE COUNT          714 a         1444 c         1671 g          800 t
ORIGIN      
        1 cgccccggcg aagggccggc cctcgtggcc gggagcgtcg aacatggcga agacctggcg
       61 ctcgaaccgc gcgagatcga tcatgaagtc gatccagctc tcgcgctccg cctccggccg
      121 gccgcggtcg ggccggttct cgtccagcca ggcgggaaag cggcggccga gatcgtagag
      181 cgtgtggctc tccggtggcc tgtcctttat gtagtcggcg acgaaatcgt cgaagagcgg
      241 ctcgcccagc gcatggcaca aggccgggaa ttgctcgcgc atgcagctcg cgatccgaag
      301 gaaatagccg cgctggtaga tggcgaggcc ggcggccgcg ctcaggcgcg gcgacgcccg
      361 gagcagcgcg tcgcgccttt cgccgtcgac ccggcggggg aagacgagcg cctcgtgcat
      421 ccatcgctgg accggcccga gggcctcgcc gctcaagctt cctccaggag gttgctgtcc
      481 atcacccgag gaacgaggaa gtcgaccgga ttggacaagg agccggcgcg cgcggcctcg
      541 acggggcccg ccggcggcgg gtcgaaccgg ccggccatat agtcgttcgc cttcagcaat
      601 tcggcctggc agtcggccag agcgggaatg tcgccgtccc attctagcag ggtcgaggcg
      661 cctccggtca ggctccaggc caggcggaac aactcccaga cttccggccg gaccgtccgg
      721 tcgtgggtgt cgacgacgtg ggttccttca tgctggtggc cggcgaggtg catctggacc
      781 acatgttcgc aagggaaggc cgcgatgtag gcgaccggat cggtgccggc gttgaagcag
      841 ctgacgaaga cgttgttgac gtcgaggagc agcccgcagc cggtctcgcc ggcgaggcgg
      901 cggaggaatt cgggctcttc catcgtcgag gcggcgaagc ggacgtagct gctcggattc
      961 tccaggatca gaggccggtc gaggaagtcc tgcgcccgcc gcacccgcgc cgcgacatgg
     1021 gtcaggctct cctcggtgag cggcatgggc aggaggtcgt gcgagttgag ccccatcacc
     1081 ccggtccagc acagatggtc gctgatccag gccggcttca cctcgtcggc gaggcgcttc
     1141 agccggtcga gatagtccag atcgagcggg tcggcgcttc cgatcgacag ggacactccg
     1201 tgcaggacga ctggataatg ctcggcgacc tcgcggatga cggcgcgcgg acgcccgccc
     1261 gaatccatga aattctcgga gatcgcctcg aaccagtcga ctttcggccg acgggtcagg
     1321 atctcctcga aatggacgct gcgcaggccg aggccgaggc cgagcctggg caggtccgcg
     1381 accgggtcct cagctgcagc caccggcgcc gctcagcccg ctcgctccgc aggcgatcat
     1441 gttgcttcgg gcgctgttga cgttgctgat ccacaggtag gtcggtcccg tcgcggcgaa
     1501 gaccggcggc ggcgagccgg gcgtctcggg gagcggctga tcggccggga tatggcccgc
     1561 tgcctgcgcc tcgagcaggt cctcgcgctt ggcggcccat ttctcctcga acaccgcgcg
     1621 ggctcggagc cacacgctct ggccctggtt ggggccgttg gtgctgaagc gctccgcgtt
     1681 gatcggagtg gcgcacgagc ccagcgagcg gcagtcgttc tcgcccggcc gattcatctc
     1741 ctcgccggtg ccgtagagtc cgcagccgcc ctggtccctg caatcgttct ggacgtgaca
     1801 ggtatggtcg gtcgcggtcg agcaatagcc ctggccggcg caggcgttcg gccccagccc
     1861 gggggcgccc gccgttccga atcggtccga cgccgcgcaa ctgttgaggc ccatgcaggc
     1921 atggcttggc atgccccccg gcccgtcgcc gatcgaggtc gggaagaccg gagggttggc
     1981 gacatagggg ctggaagccg gcaccgggac tccgggggcg ggggtggcgg gagtgggcgg
     2041 ggtcgagcag gaggccattg tcagttcctc ttcgcggaag gtcttgaagg tctggccggg
     2101 gtcacgaggc ggccttccaa taggggccga cgtcgggaag atgcatcggc gcgccgtcgc
     2161 tgctcgtctg ggtgagcgcg accccgacat aataggtgac gttgccgagc gcgctcgcca
     2221 ggttcccgtt ggggtcgatt ccctgggaaa gcgccgtggc ggccgcgatc gcggcattgc
     2281 catattcggt cagccccgcg aagctgtcct ccggcgctcc gaggttcacg ttctcgaaca
     2341 acggccccat catcctgcag gcgtgggggc cgctcggaat ctcgatcttg cggatcgtct
     2401 ggatatattt gtccagcacc cagatcatcg agcggtgcag cccttcgttg aagaacagct
     2461 tctggttggc cggcggaacg cggtagatcg tctcgcacat gatcagcatg tactggaaga
     2521 gcgcgctgct gaagtcggcg atcgcccgat actggaaggg atagtcgatg ctccggggac
     2581 tgtcgggaag atcgatgagc acgcccgcgg cgagcagcgc gtcgtcggtc accgacggag
     2641 tgatcggctg gggcggctcc ggctcggggg gaagcacctc ggtgcagccc gcataatcgg
     2701 cgaactgcgc ctgaagcgtg aggaacttca tgtagtgcga agcctcgtcc ttggtcggat
     2761 cgtcgtccgg cgacgggccg acgtccggca cagggcagcc ctcgccctgg tcgcagatcg
     2821 tgtcgatcgc cgccgccgcg tcggcgcgcg aggcgatgga gatcagctcc gtgggcccgg
     2881 aatggctgtc ggcacggtcg gggaagaccg cgacctcggc cgcgcccggc ttgccgtcgg
     2941 cccagctcgg cgccggcacc ggcggcgccg gcttccaggg atcgaagccg gacccgggat
     3001 agacggtgtc gacgttgttc ggcgagtaat tgtagggctg gatggcgctc gccgccgccc
     3061 cctgctggaa gtccgcgtcg gtcacgaagc gggtcctcag caggcagcgt atgtaggaat
     3121 agaactggcc gatcgtgtcc cagttgcggt cctgcggaag cgcgtcccac tgctcgggat
     3181 attcgatctg gaggaagtgc cagagctgct cgaagctcag ccgctcgagc gggatcagaa
     3241 ccggagtcga tccgtccggt ccggccgggc ccttgggatt gtgatagggc aggcccgtgg
     3301 gatagctgga cggcgccctc ccgtagagcc gcggcggcac gcccatcgac cacaggatgt
     3361 tcgacgacag cgacatgtgg agcatctcct ccaccgccac gctcatgatc agcccgcccg
     3421 ccttgttggc gtagagctgg gcgtcgctga tcgtctcgcc gctctcgctg ttccggtgca
     3481 gcgagtagta ggtgtagagg tagatgggga tggtcgcgag ctcgatctcg acggccgtct
     3541 ggagattggc cttgagctcg gcgagcacct cctcgcggct gctggtcagg tccagccaga
     3601 cgacgaaggc ctcctcgacc ggggtcagcg gagtgatcgg atcgggctgt cccgcggcga
     3661 tgctgctcgg ctgctcgttc atgactgggc ttccgtgccg agcccggccg tcgacggcga
     3721 caagccctcg accctggtca gcgtgttcga ggtgacgtgg gggaatttgt acacggtccc
     3781 gtggagcggg atctccaggg tgatggtctg gctatattgg agctggtcgt agccgtcgct
     3841 tccttccagt tgctcgagcc agtaggtcgc cgaatagcgc atgacgttgg catggcgctc
     3901 ctcgtaaccg atgttggtca ccatgccctt gctgttggcg aaggccgtgt cgacctggaa
     3961 cttgatgtag ctgacgacat tcgcctggcc gagagcctcg gcgagcggcg cgttcgggtt
     4021 ggccgtgtag gcggtgttcg ggttgcccac gcctggggtg gtataaggcg ccgggccgac
     4081 acccgccggg atcaccgtgc cggtcaggtc cgggatcgaa ggcgtgccgc tctgcggctg
     4141 aagcggaaag ggcggcgctt ctccgagcgc cagcaccgaa ttgccgtgcg ggaccgagat
     4201 ctgcttaacg agggtcggaa agccggtggg aggggtgctc ccgggcactt ccgggcccgt
     4261 gctgtagggt ccttcgagct ggacgctgct ggacagcacc agccatgagc cgttttccgc
     4321 atggacgagc gtgttggtgg ccggagttcc cgatgcctcg gagatataga tgcgctgctc
     4381 gtagaagagc gggttgcaga cctggacccc ggtcccgccg cgattggcgg cattgccgtg
     4441 gatgggggag aaggtgatct cctcgtaata ttgcatgttc ttgaggatga agccttcggg
     4501 cgtggtgacg gaatccgact gcggaagcac catcagattg taggaataag ggctgtcggg
     4561 cccgcccttc ccgttcgggt acggctggtt ggtccacgtt cccacgagga gctgaagcgc
     4621 ccccagcac
//
LOCUS       DASWNU010000569         5057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_48134, whole genome shotgun sequence.
ACCESSION   DASWNU010000569 DASWNU010000000
VERSION     DASWNU010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48134"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VGB57_11275"
     CDS             complement(<1..496)
                     /locus_tag="VGB57_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEY0131974.1"
                     /translation="MTEAEPLGHEPWPLRAVILLILGAALALAVHLLTRGSEAWQWTE
                     SPLRLGAAAVLAAGGIVFAVSLERPRWLWSVLFAAGAGLVVGFIVYWNGSPESWGAGE
                     AWQLVSALLAVVIAVPLFQASRDGGRWNLDYRLVHAHILTNVVLWLVAWGFVLITFLL
                     ILLLS"
     gene            586..1698
                     /locus_tag="VGB57_11280"
     CDS             586..1698
                     /locus_tag="VGB57_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEY0131975.1"
                     /translation="MARRFLWVVAGLTMLVMAAALLYRLFGAELMRFTMVPSAPFEGG
                     PAPAPKAYADKRLWIARPDLPNSPGLWMPTGLKREGTPVASAFFIHPTSYLQTAQWNA
                     PLADPESQWRAKLFVRSQASVFNGVADVWAPKYRQATFGAFLTTKAEAQKALDFAYRD
                     VVAAFEQFLVEAPKNRPIILAGHSQGSLHLMRLLADRIAGKPEAKRVVAAYVIGWPVS
                     IPADLPSLGLPACEKREQSGCVVSWQSFAEPADPKQVTDVYDKSAGPTGISRAGSPML
                     CTNPLTGAPGGEAPASANLGTLVPNEALTQGELKAPGVPARCDVRGFLLIGEDAPELG
                     PYVLPGNNYHVYDYALFWSNLRADAAARVAAFGGGK"
     gene            1808..2269
                     /gene="ruvX"
                     /locus_tag="VGB57_11285"
     CDS             1808..2269
                     /gene="ruvX"
                     /locus_tag="VGB57_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY0131976.1"
                     /translation="MITTSAADYREALPEGGRLLGLDVGTKTIGLALCDADWTIATPA
                     ELLQRGKFGKDLERLRTLAVAQRVTGLVVGLPLNLDGSESPRTQSVRAFARNLEPTGF
                     PILLWDERWSTQAVTRTLLDADASRARRAELVDKMAAAYILQGAIDALTMG"
     gene            complement(2548..3342)
                     /locus_tag="VGB57_11290"
     CDS             complement(2548..3342)
                     /locus_tag="VGB57_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0131977.1"
                     /translation="MWKLLLIPVFLYVALLLLVFAFQGRLLFPAGAVGAAPPLPPGAE
                     RLALDTSDGHRLHGVHIRPAAAPSGTLILGFGGNAWNAETAAAYLHGLFPEADVVAFH
                     YRGYRPSTGSPSARALLDDAPLVHDLAIGRVRPARTVVIGLSVGSGVAAGLASQRSLD
                     GLVLVTPFDSMSRVAARQYPWLPVRWLFRHEFRAADWLRDSRVPTAIIAAERDTLIPP
                     ARTDGLRRSVGNLVYDRTIPGAGHNDIYDRPEFRAAIREALRRVGG"
     gene            complement(3353..3706)
                     /locus_tag="VGB57_11295"
     CDS             complement(3353..3706)
                     /locus_tag="VGB57_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0131978.1"
                     /translation="MTARSILIVEDEPLIAMMLEDFLDSLGHTVAGTCDTVADALARV
                     VQGGFDIAIIDVNLNGEQVWPVADRLAAQGVPYILATGGHIEPPPAQHASVPVLSKPF
                     TLDAIEPALTEACGG"
     gene            complement(3909..>5057)
                     /locus_tag="VGB57_11300"
     CDS             complement(3909..>5057)
                     /locus_tag="VGB57_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0131979.1"
                     /translation="QATKMQAVGQLAGGVAHDFNNILTAIIGHCDLMLMRHAPGDSDY
                     DDIQQIRSNSNRAASLTRQLLAFSRQQTLRPQVLQLPDVIAEVSTLLKRLLGERVKLQ
                     VKHGRGLGAVRADPGQLEQVIVNLAVNARDAMTVKDDQSVGLLTLHTYAVTADDVRRM
                     GSEILPIADYTALRVSDTGTGIPPHLLPKIFEPFFTTKEVGKGTGLGLSTVYGIVKQS
                     GGFIFAESEPGKGTSFVIYLPVHRAEAAAKPGRKAREPAGELWGTGTILLVEDEATVR
                     AVAERALTRHGYTVLLAENGEAAIEILDREKRVDLMISDVVMPTMDGPTTAREARLRQ
                     PDLPILFISGYAEEQLRQSIDLERVAFLAKPFSVQTLAEATRDALVMK"
BASE COUNT          821 a         1662 c         1783 g          791 t
ORIGIN      
        1 cggagagcag aaggatgagc aggaaggtga tgaggacgaa gccccaggcg acgagccaga
       61 ggacgacgtt ggtcaggata tgcgcgtgga ctaggcggta atcgaggttc cagcggccgc
      121 cgtcccgcga cgcctggaac agcgggacgg cgatgacgac ggcgagcagt gcggaaacga
      181 gctgccaggc ctcgcccgcg ccccagcttt cgggcgagcc gttccagtag acgatgaacc
      241 cgacgacgag gcccgcgccg gcggcgaaga gcaccgacca gagccaccgc ggcctttcaa
      301 gcgagactgc gaagacgatc ccgcccgcag ccagaacggc ggcggctccc agccgcagcg
      361 ggctctcggt ccattgccag gcctcggacc ctcgggtgag caaatggacg gccagtgcca
      421 aagcggcgcc gagtatgagg aggatgaccg ctcgaagggg ccagggctcg tggccgaggg
      481 gctccgcttc cgtcatttca ttctccggcc gcgttgcttc tagcgcttgc attcgaatct
      541 accatccgct tgtgcagcca cgatgcaggc ggggaggatt tgatgctggc gcggcggttc
      601 ctgtgggtgg tggcggggct gaccatgctc gtcatggccg cggcgctcct ctaccggctg
      661 ttcggcgccg agctgatgcg gttcacgatg gtgccctcgg cccccttcga aggcggcccg
      721 gccccggccc caaaggccta tgccgacaag aggctgtgga tcgcccggcc ggacctgccg
      781 aacagccccg gcctctggat gccgacgggg ctcaagcgcg aaggcactcc ggtggcttcg
      841 gccttcttca tccaccccac ctcctatctg cagacggcgc agtggaacgc tccgctcgcc
      901 gacccggagt cgcaatggcg ggcgaagctc ttcgtgcgca gccaggccag cgttttcaac
      961 ggggtcgcag acgtctgggc gccgaaatac cggcaggcga ccttcggcgc cttcctcacc
     1021 accaaggcgg aagcgcaaaa ggcgctcgac ttcgcctatc gcgacgtcgt cgcggccttc
     1081 gaacagttcc tcgttgaagc gccgaagaac cggccgatca tcctcgccgg ccacagccag
     1141 ggcagccttc acctgatgcg cctgctcgcc gatcggatcg ccggcaagcc ggaggcgaaa
     1201 agggtcgtcg cggcttatgt gatcggctgg cccgtctcga ttcccgccga tctcccttcg
     1261 ctcggcctcc ccgcctgcga aaagcgggag cagtcgggct gcgtggtcag ctggcagagc
     1321 ttcgccgagc ccgccgaccc caagcaggtg accgacgtct acgacaagtc cgccgggccg
     1381 accggaatat cgcgcgccgg atcgccgatg ctgtgcacca acccgctgac cggcgcgccc
     1441 ggcggcgagg cgccggcctc cgccaatctc ggaacgctcg tccccaacga ggccctgacc
     1501 cagggcgagc tcaaggcgcc cggcgtcccg gcccgctgcg acgttcgcgg cttcctgctg
     1561 atcggcgagg acgctcccga gctcggcccc tacgtgcttc cgggcaacaa ttaccacgtc
     1621 tacgattacg ccctgttctg gtcgaacctg cgcgcggacg cggcggctcg ggtggcggcc
     1681 ttcggcggcg ggaaatgacg atcgctgtcg cctggcgcac ccgtcatccc ggcgaaagcc
     1741 gggacctggt ggaggtcgcc gggacttttc gaactgaggt cccggctttc gccgggatga
     1801 cggggatatg atcaccacgt cggccgccga ttaccgcgaa gcgctgcccg aaggcggccg
     1861 tctcctcggc ctggacgtcg gcaccaagac gatcggcctc gcgctctgcg acgccgactg
     1921 gacgatcgcc accccggccg agctcctcca gcgcggcaag ttcggcaagg acctggaacg
     1981 gcttcggacg ctggccgtgg cgcagcgagt caccggcctc gtcgtcggtc tacccctcaa
     2041 cctcgacggc agcgagtcgc cgaggaccca gtcggttcgg gccttcgccc gcaacctcga
     2101 gcccacgggc tttccgatcc tgctctggga cgagcgctgg tcgacccagg ccgtgacccg
     2161 caccctcctc gacgccgacg ccagccgggc ccgccgggcc gagctggtcg acaagatggc
     2221 cgccgcctac atcctccagg gcgcgatcga cgctttgacg atgggctgag ggccggattt
     2281 tacgcacctg caacttccga aacggatctt cctgaaggag atcggcgcct gatgttccga
     2341 tattgttctt cctgccgagt cgcttatcgg cagggctctt tgacattgtg agatgagatt
     2401 ggagacggag caggtgccgc cccgctccgt tcgcgcatgg cgtgtcgaag cgcctgcttc
     2461 tctctggcca agaggaggaa aatgcttcga tgagcgaacc aacggggcgg ggcgcggcga
     2521 attccaagtc ggacggctgc cttccagcta gccgccgaca cggcggagcg cttcccgaat
     2581 cgcggcccgg aactcgggac ggtcgtatat gtcgttgtgc ccggcaccgg ggatggtgcg
     2641 atcgtagacc aggttgccga ccgaccgccg caggccgtcc gtgcgtgccg gcgggatcag
     2701 ggtgtcgcgc tccgcggcga ttatcgcggt gggaacccgg gagtcgcgaa gccagtcggc
     2761 ggcccggaat tcgtgccgga acagccagcg caccggcagc cagggatatt ggcgcgccgc
     2821 gaccctgctc atcgaatcga acggggtgac cagcaccagc ccgtccagcg acctttggga
     2881 ggcaaggccg gccgcgaccc cgctgcccac gctgaggccg atcacgacgg tcctggcggg
     2941 acggaccctg ccgatcgcga gatcgtggac gagcggcgca tcatcgagca gcgcccgagc
     3001 gctcggcgag ccggtggaag ggcggtagcc gcgatagtgg aaggccacga cgtccgcttc
     3061 ggggaacagg ccgtgcaggt aggcggcagc cgtctcggcg ttccaggcgt tgccgccgaa
     3121 gccgaggatc agggttcccg aaggcgccgc ggcgggcctg atatggaccc cgtggaggcg
     3181 atggccgtcg ctcgtatcga gggcgaggcg ctcggcgccg ggcggaagcg gtggggccgc
     3241 gcccacggcg ccggccggaa agagcagccg cccctggaag gcgaagacga ggaggaggag
     3301 cgcgacgtag aggaaaacgg ggatgaggag gagcttccac atggtcccgg tcctagccgc
     3361 cgcatgcctc cgtcagcgcc ggctcgatcg cgtcgagggt gaagggcttg gagagcaccg
     3421 gcacgctcgc atgctgcgcg ggcggcggct cgatatgacc gccggtggcg aggatgtagg
     3481 ggacgccctg ggcggcgagc cggtcggcga cgggccagac ctgctcgccg ttcagattga
     3541 cgtcgatgat ggcgatgtcg aagccgccct ggacgacccg ggcgagcgcg tccgcgacgg
     3601 tgtcgcaggt cccagcgacc gtatgcccga gcgagtcgag gaaatcctcg agcatcatcg
     3661 cgatcagcgg ctcgtcttcg acgatgagga tggaacgggc ggtcacggag cttcgcttaa
     3721 ggcgtaacta tcgtttgcga aacaagtttt ttgatcgcct tgcctccctg cgccgaaggc
     3781 gtggggaggg gtaccatgcg aagcatggtg gaggggtagc tgacgaggcc cgggcaatta
     3841 ccctccacca cccttcgggt ggtccccctc cccatcgctg cgcgatgggg aggcaaattc
     3901 acgcctcgtc acttcatcac caaggcatcc ctcgtcgcct cggccagcgt ctggaccgaa
     3961 aaaggcttgg cgaggaaggc gacgcgctcg aggtcgatcg actggcgcaa ttgctcctcg
     4021 gcatagccgg agatgaagag gatcggcagg tccggctggc ggagcctcgc ctcgcgggcg
     4081 gtggtgggtc cgtccatcgt cggcatgacg acgtcggaga tcatcaggtc gacgcgcttc
     4141 tcgcggtcga ggatctcgat cgccgcttcg ccattctccg cgaggaggac ggtgtagccg
     4201 tggcgggtga gcgcacgctc ggcgacggcc cggaccgtcg cctcgtcctc gacgaggagg
     4261 atggtgccgg tgccccacag ctcgccggcc ggctcgcgcg ccttgcgtcc cggcttggcc
     4321 gccgcctcgg cgcggtggac ggggagatag atgacgaagc tcgtcccctt ccccggctcg
     4381 ctttcggcga agatgaagcc gcccgattgc ttgacgatcc cgtagacggt ggaaagcccg
     4441 aggccggtcc ccttgcccac ctccttggtg gtgaagaagg gctcgaagat cttggggagg
     4501 aggtgcggcg ggattccggt gccggtgtcg gaaacccgga gcgccgtata atcggcgatc
     4561 ggcaatatct cgcttcccat ccggcgcacg tcgtcggcgg tgacggcgta ggtgtggagg
     4621 gtgagcagcc cgacgctctg atcgtccttg accgtcatcg cgtcgcgggc gttgaccgcg
     4681 aggttgacga tgacctgctc gagctgcccg ggatcggccc ggaccgcgcc gaggccacgg
     4741 ccgtgcttga cctgcagctt gacccgctcg ccgagcagcc ttttcaggag cgtcgagact
     4801 tcggcgatga cgtcggggag ctggagcacc tggggccgaa gggtctgctg ccgcgagaag
     4861 gcaaggagct ggcgggtcag gctggcggcc cggttggagt tggagcggat ctgctggatg
     4921 tcgtcatagt cgctgtcgcc cggcgcgtgg cgcatcagca tgagatcgca atggccgatg
     4981 atggcggtga ggatgttgtt gaaatcgtgg gcgactccac cggcaagctg gccgaccgcc
     5041 tgcatcttgg tggcctg
//
LOCUS       DASWNU010000570         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_99907, whole genome shotgun sequence.
ACCESSION   DASWNU010000570 DASWNU010000000
VERSION     DASWNU010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99907"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VGB57_11305"
     CDS             complement(<1..1431)
                     /locus_tag="VGB57_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0131980.1"
                     /translation="MYHAPVPLDVDPQELEPAELADESVGADASSQRLRDAHLHNGQA
                     QTRLLFAPFFALTGAGAALLVAWAMLGTTRIELIGGWLALVGFANWTYFRSAKTEGAA
                     AESRSAEGRPLVRAIGEAVGLAAIWSAFPTYAFATQPHEVQVVIGGAMGAMIMAAIGL
                     ASVPAAAFAWIATLTAALCCAYYLGSSQLNPTFGASIVGVAAVAIFGVARLTRWTFGQ
                     LQTIANVRTQAESVRLLLKEYEHRGVGWLWQVDVENRVVYISSRMTSLLGRSASQLIG
                     HSLPATLGGNSALGRTLLARAPFASLEMELKTRRGTRWISLAGDPIIDMAGQFQGFRG
                     VGSDITEVRRTQERLTNLANMDVLSGLPNRGRVRQLLGEALSTSQSSNVPCAIMFLDL
                     DGFKPVNDTFGHPKGDAVLKSVSQRLVKEAGESGHVGRMGGDEFAIVMRDAQSRKKVD
                     DLARRIIAAVAEPYHIDKAEIRIGVSI"
     gene            complement(1643..2305)
                     /locus_tag="VGB57_11310"
     CDS             complement(1643..2305)
                     /locus_tag="VGB57_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY0131981.1"
                     /translation="MPATDPSIDAYIARQADFARPILEHLRSAVHAACPEAEETLKWG
                     MPHFLYKGQMLAGMAAFKAHATFGFWRSKDVLGETGLRETGPGETGLGETGLGETGAE
                     REAMGQFGRLTSVADLPPDDELQTLIRNAMALTDVGKRPARPRGAPKPALETPPELET
                     ALSANPAARATFDGFPPGCRREYVEWVAEAKRPETRDRRIAQAVEWMAEGKKRNWKYE
                     KC"
BASE COUNT          388 a          827 c          878 g          416 t
ORIGIN      
        1 gatcgagact ccgatccgga tctcggcctt gtcgatatga taaggctcgg cgacggcggc
       61 gatgatccgg cgggcgaggt cgtccacctt cttgcggctc tgggcgtcgc gcatgacgat
      121 ggcgaactcg tcgccgccca tccgcccgac atggcccgat tcgccggctt ccttgacgag
      181 gcgctgcgag acgctcttca ggaccgcgtc gcctttcgga tggccgaacg tgtcgttgac
      241 cggcttgaac ccgtcgaggt cgaggaacat gatcgcgcag ggcacattgc tcgactgcga
      301 ggtggagagc gcctcgccga gcagctggcg gacgcggccg cgattgggca ggcccgacag
      361 aacgtccatg ttggcgaggt tggtcagccg ctcctgggtg cggcggacct cggtgatgtc
      421 cgagccgact ccgcggaagc cctggaactg gccggccatg tcgatgatcg gatcgccggc
      481 gaggctgatc cagcgcgttc cgcggcgggt cttcagctcc atctcgaggc ttgcgaaggg
      541 cgcgcgggcg agaagggtgc ggccgagagc gctgttgccg ccgagagtcg cgggaagcga
      601 atggccgatc agctggctgg ccgagcggcc gagcagggag gtcatccgcg aagagatgta
      661 gacgaccctg ttctcgacat cgacctgcca cagccagccc actccgcgat gctcatattc
      721 cttgagcagg aggcggaccg attcagcctg ggtgcggacg ttcgcgatgg tctggagctg
      781 gccgaaggtc cagcgggtga gccgggcgac cccgaagatc gcgaccgcgg cgactccgac
      841 gatcgaggcc ccgaaggtcg ggttgagctg cgagctgccc agataataag cgcagcacag
      901 ggccgcggtc agagtcgcga tccaggcgaa ggcggcggcc ggaacggagg cgaggccgat
      961 cgccgccatg atcatcgcgc ccatcgcgcc gccgatgacg acctgcacct cgtgaggctg
     1021 ggtggcgaaa gcataagtcg ggaaagcgga ccagatggcg gccaggccga ccgcctcgcc
     1081 gatcgctcga accagcggcc ggccctcggc ggagcggctc tcggccgcgg cgccctcggt
     1141 cttggcggag cggaaatagg tccagttggc gaacccgacc aaagcgagcc agccgccgat
     1201 cagctcgatc cgggtcgtcc cgagcatcgc ccaggcgacc agcagggccg ctccggcgcc
     1261 ggtgagggcg aaaaagggtg cgaagaggag gcgggtctga gcctgcccgt tgtggaggtg
     1321 ggcgtcgcga agcctttggc tgctcgcgtc ggcaccgacg ctctcatccg ccagctcggc
     1381 cggctccaat tcctgcgggt cgacatccaa gggaacgggc gcgtgataca tgggaatcct
     1441 gcgtgctgcc gggttggaca gtccctacgc gcaagagttt tgatggagtg ttaaggtcgc
     1501 gggagaatgc ggtgagccgt cggcgcctcg acattttcct cagcgcgcgt tccccgtcat
     1561 cccggcggaa gccgggacct cgtggaagaa agggcgcctt atcctcgccg aggtcccggc
     1621 tttcgccggg atgacgtgcc tctcagcatt tctcgtattt ccagttccgc ttcttgccct
     1681 ccgccatcca ttccaccgcc tgggcgatac gcctgtcgcg agtctcgggc ctcttcgcct
     1741 cggccaccca ctcgacatat tcgcgccggc agcccggtgg aaagccgtcg aacgtggccc
     1801 gggcggcggg gtttgcggag agggcggtct cgagctccgg cggagtctcc agcgccggct
     1861 tcggcgctcc tctgggccgt gcggggcgct tccccacgtc ggtaagcgcc atcgcattgc
     1921 ggatcagggt ctgaagctcg tcgtccggcg gcaggtccgc caccgacgtc aggcgcccga
     1981 attgccccat cgcctcgcgc tcggcgccag tctccccgag cccggtctcc ccgagcccag
     2041 tctccccggg cccggtctcc cggagcccgg tctccccgag cacgtccttc gagcgccaga
     2101 aaccgaaggt cgcgtgcgcc ttgaaggcgg ccatgccggc gagcatctgc cccttgtaga
     2161 ggaaatgcgg catgccccac ttcagagtct cttccgcctc cgggcaggcg gcgtggacgg
     2221 cggagcgaag atgctcaagg atcggccggg cgaaatccgc ctggcgggcg atgtaggcgt
     2281 cgatgctcgg gtcggtcgcg ggcatcgagg gattatagac ccgttccgcg gctgaatcga
     2341 cccggcgccg gttcagcgcc tgactttgat ccttccgatc agccgttcgt cctcctgctt
     2401 gcctccgggc agaagctcga cgtcgatcac ctcctcgcgc tcctgcggcc ggccgaaatc
     2461 gtggatcagg tgggcggtga ttcgggaagg gccgttgggg ttcagctcg
//
LOCUS       DASWNU010000571         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_307166, whole genome shotgun sequence.
ACCESSION   DASWNU010000571 DASWNU010000000
VERSION     DASWNU010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307166"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VGB57_11315"
     CDS             complement(<1..286)
                     /locus_tag="VGB57_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0131982.1"
                     /translation="MTEHFLQGRIALVTGASRGIGAATAEALAREGAHVVLTARTAGD
                     LEAAEDRIHRAGGTATIAPLDLIETDSIARLATAVADRWGRLDILMLNAAT"
     gene            complement(374..1831)
                     /gene="purF"
                     /locus_tag="VGB57_11320"
     CDS             complement(374..1831)
                     /gene="purF"
                     /locus_tag="VGB57_11320"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0131983.1"
                     /translation="MLTTHPFDDDKLREECGVFGIWGSDSASAVAALGLHALQHRGQE
                     AAGITSFDGRNFHSHRAMGHVAGNFDKDEVIRRLAGAAAIGHVRYSTAGETALRNVQP
                     LFAELASGGFAVAHNGNISNAVRLRRDLNRRGSIFQSTSDTEVIIHLVATSTYRTLLD
                     RLIDACKQVEGAYSLICLTGEGMIACRDPLGIRPLVMGRLGDSYIFASETVALDVVGA
                     TYLRTVEPGEIVIVSAQGVRSHKPFQSVRPRPCIFEHIYFSRPDSVLDGASVYQVRKE
                     IGAELARENPVEADYVVPVPDSGVPAAIGYAQASGIPFELGIIRSHYVGRTFIQPSDA
                     VRHLGVKLKHNANSALIQGKRLVLIDDSIVRGTTSLKIVQMLREAGAREVHMRIASPP
                     TRHSCFYGVDTPERAKLLAAQMNVAQMNDYIKADSLAFISIDGLYRALGEDLREEAQP
                     QYCDACFTGAYPTRLTDQEDVEAPDQLALLAERYA"
     gene            1972..2487
                     /locus_tag="VGB57_11325"
     CDS             1972..2487
                     /locus_tag="VGB57_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131984.1"
                     /translation="MKKILPLLLLLAACGRDDAPAGNQAAPESGGPAASKPGSSASSP
                     ASAQARLTGLYEGGAGAQKNQMCIVEKGNDTEFGLVVWGGNMHSCSGAGRAVRRGERL
                     TLTMTGDETCTIDATLRDGIVQLPDSLPLGCAYYCGARASLAGARFSRTGSSEADSKK
                     ARDIAGDPLCD"
     gene            2628..3047
                     /locus_tag="VGB57_11330"
     CDS             2628..3047
                     /locus_tag="VGB57_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0131985.1"
                     /translation="MPHALHSDFATPESGGKSYTISDLCEEFSVTPRALRFYEDEGLI
                     SPHRQGLSRVYGWRDRARLAWILRGKRVGFSLAEIREMIDLYDLGDGRASQRRVTLEK
                     CRGRIDLLTKQRHDIDATIAELERFIAMVEAADSAGN"
     gene            3056..>4213
                     /locus_tag="VGB57_11335"
     CDS             3056..>4213
                     /locus_tag="VGB57_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0131986.1"
                     /translation="MPSYTAPVRETRFVLEDVLGLERYSNLPGFENATPDMIEAILTE
                     GGRFASEVLAPLNRIGDEKGCVRHEDGSVSTPPGFKDAWDQFTAGGWTTLSAPEEYGG
                     QGLPQIVGTAVTEYLLSANQAFEMYHGLTAGAVASILTKGSDEQKAKYLPNMVAGKWT
                     GTMNLTEPHCGTDLGLLRTRAVPQGAGTDGVAESYRISGTKIFISSGEHDLAENIIHL
                     VLAKIEGAPDNVKGISLFIVPKRILDDNGTLIANGVSCGSIEHKMGIHGNSTCVMNYD
                     EATGYLVGEPEKGLAAMFIMMIAARLGVGLQGLAQGEVAYQNAVAYAKDRRQGRALVP
                     DARDPNARADPIIVHPDVRRMLMEMKAFNEAARALVLWGALQVDISRRAQTE"
BASE COUNT          707 a         1412 c         1418 g          676 t
ORIGIN      
        1 aagtcgcggc gttgagcatg aggatgtcga gcctgcccca tcgatcggcc acggccgtcg
       61 ccagcctggc gatgctgtcc gtttctatga ggtcgagcgg agcgatggtg gcggtgccgc
      121 cggcccggtg gattcgatcc tccgcggctt ccaggtcccc agcggtccgg gcggtgagga
      181 cgacgtgggc gccttcgcgg gccagcgcct cggcggtggc ggcgccgatg ccgcggctgg
      241 cgccggtgac gagggcgata cggccctgga gaaaatgttc ggtcatatcg tcatcccggc
      301 gaaagccggg acctcaggac aagtaggcac ggtgggtctc cccgaggtcc cggcttccgc
      361 cgggatgacg gtgtcaggca taacgctccg ccagcagcgc gagctggtcg ggcgcctcga
      421 catcctcctg gtcggtgagc cgggtcggat aggcgccggt gaagcaggcg tcgcaatatt
      481 gcggctgggc ctcttcgcga aggtcctcgc ccaaggcccg gtagaggccg tcgatcgaga
      541 tgaaggcgag gctgtccgcc ttgatgtagt cgttcatctg ggcgacgttc atctgcgccg
      601 cgagcagctt cgcccgctcc ggggtgtcga ctccgtagaa gcagctgtgc cgggtcggcg
      661 gcgaggcgat ccgcatatgg acctcgcggg cgccggcctc gcggagcatc tggacgatct
      721 tgaggctggt cgtgcctcga acgatcgaat cgtcgatcag caccagcctc ttgccctgga
      781 tgagcgccga attggcattg tgcttgagct tgaccccaag gtggcggacg gcgtcgctcg
      841 gctggatgaa ggtccggccg acataatggg agcggatgat cccgagctcg aacggaatcc
      901 cgctcgcctg ggcgtagccg atcgcggcgg gaacgccgct gtccggcacc ggcacgacgt
      961 aatcggcctc gaccggattc tcgcgggcga gctccgcccc gatctccttc ctgacctggt
     1021 agacggaagc gccgtcgagg acgctgtcgg gcctcgagaa atagatatgc tcgaagatgc
     1081 aggggcgggg cctgacgctc tggaacggct tgtgcgagcg cacgccctgg gccgagacga
     1141 tgacgatctc gcccggctcg acggtgcgca ggtaggtcgc tccgaccacg tcgagggcga
     1201 cggtctcgga ggcgaagatg taggaatcgc cgagccggcc catgaccagc ggccggatcc
     1261 cgagcggatc gcggcaggcg atcatgccct cgccggtcag gcagatgagc gaataggccc
     1321 cttccacctg cttgcaggcg tcgatcagcc ggtcgagcag ggtccggtag gtcgaggtgg
     1381 cgacgaggtg gatgatgacc tcggtgtcgg atgtcgactg gaagatcgag ccgcgccggt
     1441 tgaggtcgcg cctcaggcgc accgcgttcg agatgttgcc gttatgagcg accgcgaagc
     1501 cgcccgaggc gagctccgcg aacaggggct ggacgttcct cagcgccgtc tcgccggccg
     1561 tcgaatagcg gacgtggccg atggcggcgg cgccggcgag ccgccggatc acctcgtcct
     1621 tgtcgaaatt gccggcgaca tggcccatcg cccggtgcga atggaagttg cgcccgtcga
     1681 agctggtgat cccggccgct tcctggccgc gatgctggag ggcgtggagg cccaatgcgg
     1741 ccaccgccga ggcgctgtcg gagccccata tgccgaagac cccgcattcc tcgcggagtt
     1801 tatcgtcgtc aaaaggatgc gtggtcagca tgtgagaaac gcccgctcgc tcgttctgaa
     1861 ttgccccacc gccatatagg aagcgatcgc gcctttgtcc ccccgggcgg acgattttga
     1921 tgtggattat ggaacggctc gccccgtcgg gattcttgcc ctgagacagt catgaagaaa
     1981 attctccccc tcctcctcct tctcgccgcc tgcgggcgcg acgacgcgcc ggccggcaac
     2041 caggccgcgc ccgagtcggg cggacccgcc gcttcgaagc cggggtcatc cgcctcgtcc
     2101 ccggcgtcgg cccaggctcg gctcaccggc ctctacgagg gcggcgcggg cgctcagaag
     2161 aatcagatgt gcatcgtcga gaagggcaat gacaccgagt tcggactggt cgtctggggc
     2221 ggcaacatgc acagctgctc gggcgccgga agggctgtgc gcaggggcga gaggttgacc
     2281 ctgacgatga ccggcgacga gacctgcacg atcgacgcga ctctcaggga cggcatcgtc
     2341 cagctcccgg atagcctgcc gctgggctgc gcttattatt gcggcgcccg cgccagcctg
     2401 gccggcgccc gcttcagccg gaccgggtcg agcgaggcgg attcgaagaa ggccagggac
     2461 atagccgggg acccgctctg cgactaaccc gtcatcccgg cgaaagccgg gacctcggga
     2521 cgacaggtct cggcgggtct cgccgaggtc acggctttcg ccgggatgac gctttctccg
     2581 cgtccgcctt attgacgttg acgtgaacgt aaactactcc ggccggcatg ccccacgccc
     2641 tccattcgga cttcgccacg cccgaatccg gcggcaaatc ctacacgatc agcgatctgt
     2701 gcgaggagtt ctcggtcact ccgagggcgc tccgcttcta cgaggacgaa gggctgatct
     2761 cgccccaccg ccagggcctg tcgcgggtct atggctggcg cgaccgggcc cggctcgcct
     2821 ggattttgcg cggcaagcgg gtcggcttca gcctcgccga gatccgcgag atgatcgacc
     2881 tttacgacct cggcgacggc cgcgcctcgc agcgccgggt gaccctcgag aaatgccgag
     2941 gccggatcga tctcctcacc aagcagcgcc acgacatcga cgccaccatc gccgagctgg
     3001 agcggttcat cgccatggtc gaggcggcgg actccgccgg aaactaagga agcttatgcc
     3061 cagctacact gcccccgtcc gcgagacccg cttcgtgctg gaggacgtgc tcggcctcga
     3121 gcgctattcc aacctgccgg gcttcgagaa cgccacgccc gacatgatcg aggcgatcct
     3181 caccgaaggc ggccgcttcg cctccgaggt gctggcgccg ctcaaccgca tcggcgacga
     3241 gaaaggctgc gtccgccacg aggacggcag cgtctccact ccgcccggct tcaaggacgc
     3301 ctgggaccag ttcaccgccg gcggctggac gacgctttcg gcgcccgagg aatatggcgg
     3361 ccagggcctg ccgcagatcg tcgggaccgc ggtcaccgaa tatctgctct ccgccaacca
     3421 ggcgttcgaa atgtatcacg gcctcaccgc cggcgccgtc gcctcgatcc tgaccaaggg
     3481 ctctgacgag cagaaggcga aatacctgcc caacatggtc gccggcaaat ggaccgggac
     3541 gatgaacctc accgagcccc attgcggcac cgatctcggc ctcctgcgca cccgcgccgt
     3601 cccgcagggg gcggggaccg acggagtggc tgaaagctac aggatcagcg gcaccaagat
     3661 cttcatctcg tcgggcgagc atgatctggc cgagaacatc atccacctcg tcctcgccaa
     3721 gatcgagggc gctccggaca atgtgaaggg catctcgctg ttcatcgtgc ccaagcggat
     3781 cctcgacgac aacggcaccc tcatcgccaa cggagtctct tgcggctcga tcgagcacaa
     3841 gatgggcatc cacggcaatt cgacctgcgt gatgaactat gacgaggcga caggctatct
     3901 cgtcggcgag cccgaaaagg gcctcgccgc catgttcatc atgatgatcg cggcgcggct
     3961 cggagtcggc ctccagggcc tcgcccaggg cgaggtcgcg taccagaacg ccgtcgccta
     4021 tgcgaaggac cgccgccagg gccgcgccct ggtccccgac gcgcgcgatc ccaatgccag
     4081 ggccgatccg atcatcgtcc atcccgacgt ccgccggatg ctgatggaga tgaaggcgtt
     4141 caacgaggcg gcccgcgccc tcgtattgtg gggcgccctc caggtcgaca tctcccgccg
     4201 cgcccagacc gag
//
LOCUS       DASWNU010000572         4305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_126359, whole genome shotgun sequence.
ACCESSION   DASWNU010000572 DASWNU010000000
VERSION     DASWNU010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126359"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGB57_11340"
     CDS             complement(<1..213)
                     /locus_tag="VGB57_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0131987.1"
                     /translation="MTARALFPADTLSLEQRRQVEALSGSVTPEQARWISGYFAGLEA
                     GIARLAGDGALPAGAAAAVRTLTVLHG"
     gene            complement(371..2119)
                     /gene="cysN"
                     /locus_tag="VGB57_11345"
     CDS             complement(371..2119)
                     /gene="cysN"
                     /locus_tag="VGB57_11345"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY0131988.1"
                     /translation="MATAPAMHPVIPAKAGTSGREVPAGLPEGPAFAGVTDDHDDPTI
                     AGQNKTLLRLLTCGSVDDGKSTLLGRLLYDSKMLFEDQLAALEADSRKSGTQGEALDF
                     ALLVDGLSAEREQGITIDVAYRFFATDRRKFIVADTPGHEQYTRNMVTGASTADLAII
                     LIDARKGVLTQTRRHSHLVHLLGIRHVVLAVNKMDMVDYAQERFEAIIDDYRAFADEI
                     GIGAFTAIPMAALTGDNIASSSPAMPWYEGPPLLEHLETVPIAAARPDSGAFLMAVQW
                     VNRPDSRFRGYAGRIAGGTVRPGDEIVILPSGRRSHVERIVTLDGDLDRAVEGQSVTL
                     TLTDEVDCSRGEVIASAASPPQVVDGLVANLVWMSDEPLAPGRSYWLKIGASIVSATV
                     SKVEHIVEVDTGRPAAARLLALNDIGRCELRLDREVAAIPYARGRELGAFILIDKLSH
                     ATAAAGMIDGFPRGAALDRATGEADRIIWLSGIAAEERAEIAARAQQKLQAIGRMSFI
                     LDEASLRENLSSDLADGPADRAEHIRRVRAVAALMSRAGLHILVAVDVPAAEAWPGRQ
                     IVPADLEQEGGDEWVI"
     gene            complement(2120..3043)
                     /gene="cysD"
                     /locus_tag="VGB57_11350"
     CDS             complement(2120..3043)
                     /gene="cysD"
                     /locus_tag="VGB57_11350"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY0131989.1"
                     /translation="MNAPATFLSRGLTHLEQLEAESIHILREVVAEAERPVMLYSVGK
                     DSAVMLHLARKAFFPAPPPFPLLHVDTTWKFRDMYALRDRVAREAGVELIVHRNPEAE
                     RRGINPFDHGSLHTDLWKTEGLRQALDLHGFDAAFGGARRDEDKSRAKERIFSFRSAH
                     HRWDPKNQRPELWSLYNSRKSRGETMRVFPLSNWTELDVWQYIHAESIPIVPLYLAAP
                     RPTVERDGMILMVDDDRFPLREGEVPVQRSVRFRTLGCYPLTGAVESRATTLPQVIRE
                     MLLATTSERQNRAIDHDGASSMERKKQEGYF"
     gene            complement(3040..>4305)
                     /gene="cysG"
                     /locus_tag="VGB57_11355"
     CDS             complement(3040..>4305)
                     /gene="cysG"
                     /locus_tag="VGB57_11355"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEY0131990.1"
                     /translation="RRGWTPADLEGAALAVADPASREEALSFVAAARAAGVPVNVIDQ
                     TDLCDVQFGTIVNRSPVVLAISTGGAAPMLGQSIRARIEAVLPLGLSAWAKAAQALRP
                     RLKARVKSFGDRRAFWEAFVERAWAGGEPDGAEALLDARPRREGRVTLVGAGPGDPEL
                     LTLKAVRALQSATVILSDDLVGPEILELARREAKRIAVGKTGRGPSCRQSDINARMVE
                     LALAGETVVRLKGGDPLVFGRATEELDACRAAGIEVNIVPGISAAQGAAAALGFSLTE
                     RREARRIQFVTGHGSDGRLPSDLDWGAIADPAATTIVYMPRATLGEFARSAVAAGLPP
                     ETPAVAIASATLPAQAQVSGTIATVAGLAGSLPPGAPVTVVIGRVARNRDAVTPAKAG
                     ASGSPPVTSQAEVPAFAGMTVSPKLRVAA"
BASE COUNT          616 a         1407 c         1647 g          635 t
ORIGIN      
        1 gccgtgaagg acggtaaggg tccggacggc ggccgctgcg cccgccggaa gggcgccgtc
       61 cccggccagc cgggcgatcc cggcttcgag gccggcgaaa tagccgctga tccagcgcgc
      121 ctgctcgggc gtgaccgagc cgctgagggc ttcgacctgg cgacgctgct cgaggctgag
      181 cgtgtcggcc gggaaaaggg ctcgagcggt catggccgat tcctcttcaa tcctccccga
      241 gatttctcgg ggagggggac caggcgaagc ctggtggagg ggccttgccg gctccgaaga
      301 acccctccac cgcgcttcgc gcggtccccc tcccccggaa aaccggggga ggaattgaag
      361 gggagatgcc ctagatgacc cattcgtcgc ccccctcctg ctcgagatcg gccgggacga
      421 tctgccgtcc cggccaggcc tcggcggccg gaacgtccac ggcgacgagg atatggaggc
      481 cggcccggct catcagggcg gcgacggcgc gcacccggcg gatatgctcg gccctgtcgg
      541 cggggccgtc ggcgagatcg ctgctcaaat tctcgcgaag cgaggcttcg tccaggatga
      601 agctcatccg gccgatcgcc tgaagcttct gctgcgccct ggctgcgatc tcggcgcgct
      661 cttcggcagc gatcccgctc agccagatga tccggtccgc ctcgccggtc gcccggtcga
      721 gcgcggcgcc gcgcgggaag ccgtcaatca tcccggcggc ggcggtggcg tggctgagct
      781 tgtcgatgag gatgaaggcg ccgagctccc ggccccgggc ataagggatg gcggcgacct
      841 cgcggtcgag cctcaactcg cagcggccga tgtcgttgag ggcgagcagg cgggcggcgg
      901 ccgggcggcc ggtgtcgacc tcgacgatat gctcgacctt gctcaccgtc gccgagacaa
      961 tgcttgcgcc gatcttgagc cagtaggagc ggccgggcgc gagcggctcg tccgacatcc
     1021 agacgaggtt ggcgacgagg ccgtcgacga cctgtggcgg cgaggcggcg gacgcgatca
     1081 cctcgccgcg ggagcagtcg acctcgtcgg tgagggtgag ggtgaccgac tggccctcga
     1141 cggcgcgatc gagatcgccg tccagggtga cgatccgctc gacgtgcgag cgccggcccg
     1201 agggcaggat gacgatctcg tcgcccgggc ggacggttcc gccggcgatc cgcccggcat
     1261 agccgcggaa tcggctgtcg gggcggttca cccactggac ggccatgagg aaggcgccgg
     1321 aatcgggccg ggcggcggcg atcggaacgg tctcgagatg ctcgagcagg ggcggccctt
     1381 cataccaggg catggccggg ctgctcgacg cgatattgtc gccggtcaga gcggccatcg
     1441 ggatggcggt gaaggcgccg atcccgatct cgtcggcgaa ggcgcgataa tcgtcgataa
     1501 tcgcctcgaa ccgctcctga gcgtagtcga ccatgtccat cttgttgacg gcgaggacga
     1561 cgtggcggat gccgagcagg tggacgaggt ggctgtgccg ccgggtctgg gtgaggaccc
     1621 ccttgcgggc gtcgatcagg atgatggcga ggtcggcggt cgaggcgccg gtgaccatgt
     1681 tgcgcgtata ttgctcgtgg ccgggagtgt cggcgacgat gaacttgcgc cggtcggtcg
     1741 cgaagaagcg gtaggcgacg tcgatggtga tgccctgctc gcgctcggcg ctgaggccgt
     1801 cgacgagaag cgcgaaatcg agcgcctcgc cctgggttcc cgacttgcgg ctatcggcct
     1861 cgagcgcggc gagctggtcc tcgaacagca tcttgctgtc gtagaggagg cggccgagca
     1921 gggtcgactt gccgtcgtcg accgacccgc aggtgaggag gcgcaggagg gtcttattct
     1981 gtcccgcgat agtcgggtcg tcgtgatcgt ccgtcacccc ggcgaaggcc gggccctcgg
     2041 ggaggcccgc cgggacttct cgtcccgagg tcccggcttt cgccgggatg acggggtgca
     2101 tggcgggagc cgtcgccatc tagaaatagc cttcctgctt cttgcgctcc atgctcgacg
     2161 cgccgtcatg gtcgatggcg cggttctggc gctccgaggt ggtggcgagg agcatctcgc
     2221 ggatgacctg cggcaaggtc gtcgcccggc tctcgacggc gccggtcagg gggtagcagc
     2281 ccagggtccg gaagcgcacc gagcgctgca ccggcacttc gccctcgcgg agcgggaagc
     2341 ggtcgtcgtc gaccatcagg atcatcccgt cgcgctccac ggtcgggcgc ggcgccgcga
     2401 gatagagcgg gacgatcggg atgctctcgg catggatata ttgccagacg tcgagctcgg
     2461 tccagttgga gagcgggaag acgcgcatcg tctcgccgcg cgacttgcgg ctgttgtaga
     2521 gcgaccagag ctcgggccgc tggttcttcg ggtcccagcg atggtgggcg gagcggaagc
     2581 tgaagatccg ctccttggcc cggctcttgt cctcgtcgcg gcgggcgccg ccgaaggccg
     2641 cgtcgaagcc gtggagatcg agcgcctggc gaaggccttc ggtcttccag aggtcggtgt
     2701 ggaggctgcc atgatcgaac gggttgattc cccgccgctc ggcctcggga ttgcggtgga
     2761 cgatcagctc cacgcccgct tcccgggcga cccggtcgcg gagcgcgtac atgtcccgga
     2821 acttccaggt cgtgtcgaca tggaggagcg ggaagggcgg cggcgccggg aagaaggcct
     2881 tgcgggcgag gtggagcatc accgcgctgt ccttgccgac cgaatagagc atcaccggcc
     2941 gctcggcctc ggcgacgact tcgcgaagga tgtggatgct ctccgcctcg agctgctcga
     3001 gatgggtgag gccgcggctg aggaaggtgg ctggagcgtt catgcggcga ctctgagctt
     3061 gggggaaacc gtcatcccgg cgaaagccgg gacctcagct tgagaagtca cgggggggct
     3121 ccccgaggcc ccggctttcg ccggggtgac ggcatcgcga ttccgggcta cccggccgat
     3181 cacgaccgtc accggcgccc ccggcggaag cgacccggcg aggccggcga cggtcgcgat
     3241 ggtcccgctg acctgagcct gggcgggaag ggtcgcggag gcgatcgcca ccgcgggcgt
     3301 ttcggggggc aggccggcgg cgaccgccga gcgggcgaac tcgccgagcg tggcccgggg
     3361 catgtagacg atggtggtgg cggcgggatc ggcgatcgcg ccccaatcga ggtcgctcgg
     3421 cagccggccg tcggagccgt ggccggtgac gaactggatg cgccgggcct cgcggcgctc
     3481 ggtcagcgag aagcccagag cggcggccgc gccctgggcg gccgagattc ccggaacgat
     3541 attcacctcg atgccggcgg cccggcaggc gtccagctct tcggtggccc ggccgaagac
     3601 cagggggtcg ccgcccttga ggcgaacgac ggtctcgccg gcgagggcga gctcgaccat
     3661 ccgggcgttg atgtccgact gccggcagga ggggcctcgg ccggtcttgc cgaccgcgat
     3721 ccgcttcgcc tcgcgccggg ccagctcgag gatctccggg ccgaccaggt cgtcggagag
     3781 gatcacggtc gccgactgga gggcgcggac ggccttcagg gtgagaagct ccgggtcgcc
     3841 ggggccggcc ccgaccaggg tgacccggcc ctcccggcgc ggccgggcgt cgagcagcgc
     3901 ttctgcgccg tccggctcgc cgccggccca ggcgcgctcg acgaaagcct cccagaaggc
     3961 gcgccggtcg ccgaagctct tcacccgggc cttgaggcgc gggcgcaggg cctgcgccgc
     4021 cttcgcccag gccgagaggc cgagcggaag gacggcttcg atcctggcgc ggatcgactg
     4081 gccgagcatc ggagcggcgc cgcccgtcga gatggcgagg acgaccggtg agcggttgac
     4141 gatggtcccg aactggacgt cgcaaagatc cgtctggtcg atgacgttga ccgggacgcc
     4201 cgcggcccgg gccgcggcga cgaagctcag cgcctcttcc cggcttgccg gatcggcgac
     4261 ggccagagct gcgccttcga gatcggccgg ggtccagccg cgccg
//
LOCUS       DASWNU010000573         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_99971, whole genome shotgun sequence.
ACCESSION   DASWNU010000573 DASWNU010000000
VERSION     DASWNU010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99971"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="VGB57_11360"
     CDS             <1..1959
                     /locus_tag="VGB57_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY0131991.1"
                     /translation="PASEARGEGDHAKHGGGVAVEASPVTPPPRYAGSPSPSAMGRQD
                     SYARAIRPIAVVQGVLVGLSFLALIQLFLRTDLSVKLVAANSHSLKPWLYKFAGAWGN
                     HEGSMLLWVTVLALAGAAVALFERRLPERTSIATLAAQAAIGLGFYAFLLFSSNPFER
                     LSPVPIEGNGLNPLLQDPGLAFHPPTLYFGYVGISIAFSFAVGALITRDVGPAFARAM
                     RPWVLIAWIFLTLGITAGSYWAYYELGWGGWWFWDPVENASLMPWLAATALLHSVTVL
                     ATRDGLRAWTVMLAVVAFSMSMVGTFLVRSGILTSVHSFAVDPKRGAFILILLALYIG
                     GALTLFGLRVGTVKEGSTFEPVSREGALVLNNLLLSAILGIVLVGTLYPLFAEAATGE
                     KLSVGPPYFNTAAGPLALILVAAMAAGPLLRWRRDRLREVARRIALPILLAALSLLAL
                     VLFVPGLRILPLLGLTLALGVGAASLAPLWKRNLLRTPLFTWGMVISHLGIAVALAGM
                     ASEAAFTRETLVAARVGDTVGVGPWSLRFESVEPLAGPNWTAVEAKLVVRRGKGEPFV
                     LRPQSRMFSSPPTETNEAAIATRLGGQLYTVIGKPDEGGRWQLRLWWKPFVTLIWLGG
                     ALVALGGLLALVGRVRRERRSARQEAWT"
     gene            1956..>2403
                     /locus_tag="VGB57_11365"
     CDS             1956..>2403
                     /locus_tag="VGB57_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEY0131992.1"
                     /translation="MKRLFLWLPLAVFLAFLVTVAIGLRKPPETEIRSRMIGKAMPDF
                     ALAPAVASHPPLAAANLRAGKPRLVNVFASWCVPCKAEAPQLMDLKRRGVPIDGVAIR
                     DRGEDVARFLAEWGDPFERIGADPASRVQFALGSSGVPETFVVDGRG"
BASE COUNT          278 a          893 c          790 g          442 t
ORIGIN      
        1 cccgcgagcg aagctcgggg ggagggggac catgcgaagc atggtggagg ggtagctgtc
       61 gaggcctcgc cagttacccc tccaccccgc tacgcggggt ccccctcccc atctgcgatg
      121 gggaggcaag acagctacgc ccgcgccatc cgccccatcg cggtcgtgca gggcgtcctg
      181 gtcggcctgt ccttcctcgc cctcatccag ctcttcctgc gcacggatct gtcggtgaag
      241 ctggtcgcgg cgaacagcca ttccctgaag ccgtggctgt acaaattcgc cggtgcctgg
      301 ggcaatcacg aaggctcgat gcttctgtgg gtgacggtgc tcgcccttgc cggcgccgcc
      361 gtggccctgt tcgagcggcg gctgcccgag cggacctcga tcgcgaccct ggccgctcag
      421 gcggcgatcg ggctcggctt ctacgccttc ctgctcttct cctccaatcc tttcgagcgt
      481 ctctcgcccg tcccgatcga gggcaacggc ctcaacccgc tgctccaaga tcccggcctc
      541 gccttccacc cgccgaccct ttatttcggc tatgtcggaa tttcgatcgc cttctccttc
      601 gccgtcggcg cgctgatcac ccgcgacgtc ggcccggcct tcgcgcgggc gatgcggccc
      661 tgggttctga tcgcctggat cttccttacc ctcggaatca ccgccggctc ctattgggcc
      721 tattacgagc tcggctgggg cggctggtgg ttctgggatc cggtcgagaa tgcctcgctc
      781 atgccctggc tggcggcgac ggccctgctt cattcggtga cggtgctcgc cacccgggac
      841 ggccttcgcg cctggacggt gatgcttgcg gtcgtcgctt tctcgatgtc gatggtcggc
      901 accttcctcg tccgctcggg aatcctgacc agcgtccaca gcttcgccgt cgatcccaag
      961 cgcggcgcct tcatcctgat cctgctcgcc ctctacatcg gcggagcgct gaccctgttc
     1021 ggacttcgcg tcggcaccgt gaaggaaggc tcgaccttcg agccggtcag ccgcgagggc
     1081 gcgctggtgc tcaacaacct gctgctctcc gcgatcctgg gaatcgtcct cgtcggcacc
     1141 ctttacccgc tcttcgccga ggcggcgacg ggggagaagc tctcggtggg gcccccctat
     1201 ttcaacaccg ccgccgggcc gctcgcgctc atcctcgtcg cggcgatggc ggcggggccg
     1261 ctcctgcgct ggcggcgcga ccggctgcgc gaggtggcca ggcggatcgc cctgccgatc
     1321 cttctggcgg ctctctccct tctggcactg gtcctgttcg tcccgggcct gcggatcctg
     1381 cccctgctcg ggctgactct ggccctgggc gtcggcgccg ccagcctggc ccccttgtgg
     1441 aagcgcaatc tgcttcgcac cccgctcttc acctggggaa tggtcatctc tcatctcggc
     1501 atcgccgttg ccctcgccgg catggcgagc gaggccgcct tcacccgcga gaccctggtc
     1561 gccgcccggg tcggcgatac cgtcggggtc ggaccctgga gcctgcgctt cgagtcggtg
     1621 gagccgctgg ccggccccaa ctggaccgcg gtcgaggcga agctggtcgt ccgccgcggc
     1681 aagggcgagc ccttcgtcct tcggccgcaa tcgcggatgt tcagctcgcc gccgaccgag
     1741 accaacgagg ccgccatcgc cacccgcctc ggcggccagc tctacaccgt gatcggcaag
     1801 cccgatgaag gaggtcgctg gcagctgcgc ctgtggtgga agccgttcgt gaccctgatc
     1861 tggctcggcg gcgccctcgt cgcgctcggc ggcctcctcg ccctcgtggg ccgggtccgc
     1921 cgcgagcgcc gctccgcccg gcaggaggcc tggacatgaa gcggcttttc ctctggctgc
     1981 cgctggcggt gttcctcgcc ttcctggtga cggtggcgat cggcctgcgc aagccgcccg
     2041 aaaccgagat ccgctcccgg atgatcggca aggccatgcc cgatttcgcg ctcgcgcccg
     2101 ccgtcgcaag tcatccgccg ctggccgcgg cgaatctgcg cgccggcaag ccccgcctcg
     2161 tcaacgtctt cgccagctgg tgcgtgccct gcaaggccga ggcgccccag ttgatggacc
     2221 tgaagcggcg cggggtgccg atcgacggag tcgcgatccg cgaccggggc gaggacgtgg
     2281 cccgcttcct cgccgaatgg ggggacccgt tcgagcggat cggcgccgat cccgccagcc
     2341 gcgtccagtt cgcgctgggc tcgtccgggg tgccggaaac cttcgtggtc gacggccggg
     2401 gcg
//
LOCUS       DASWNU010000574         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_491108, whole genome shotgun sequence.
ACCESSION   DASWNU010000574 DASWNU010000000
VERSION     DASWNU010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491108"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VGB57_11370"
     CDS             <1..473
                     /locus_tag="VGB57_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0131993.1"
                     /translation="NLHRKGEMVKAVVLDVDVEKERISLGMKQLERGGVAKGGTSAAG
                     GGVNKNETVTVTVLEVRDGGLEVQVGDDGPTGFIKRIDLGRDRDEQRPERFQVGQKFD
                     ALVIGFDRSKKPNFSIKALQIHEEKQAVAQYGSSDSGASLGDILGEALKNKGKE"
     gene            673..951
                     /locus_tag="VGB57_11375"
     CDS             673..951
                     /locus_tag="VGB57_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY0131994.1"
                     /translation="MIRSELVQLLCDDHPDLTGKEIERVVSAFYDSITDQLQQGGRVE
                     LRGFGAFSTRERDARKGRNPRTGDSVDVDAKRVPYFKPGKEMRERLNV"
     gene            1150..1236
                     /locus_tag="VGB57_11380"
     tRNA            1150..1236
                     /locus_tag="VGB57_11380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1184..1186,aa:Leu,seq:taa)
     gene            complement(1380..1691)
                     /locus_tag="VGB57_11385"
     CDS             complement(1380..1691)
                     /locus_tag="VGB57_11385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0131995.1"
                     /translation="MNQYKPRRSGRTPVAGRTQLRAQSWYSIEVTVCDVSTSGFMAEC
                     SEPVRIGSYVSLDVPGVGAVNAQVRWQLGDRMGGQFVDPISLRRCEWTAVPAEPPQQA
                     A"
     gene            1863..>2400
                     /locus_tag="VGB57_11390"
     CDS             1863..>2400
                     /locus_tag="VGB57_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0131996.1"
                     /translation="MVTRFLPAPRLLAATLLLAGCGGEETGPISISAIGGQPRIANPS
                     RDRLDPPSAILIEAAAQGLVRFDAGGEIEPALAQRWIVSDDGLRYTFRLGEASWGGRR
                     ITAEQVVARLRAAVAPASRNPLKPVLGAVDEIVAMTDDVLEISLKSPRPNFLQLLAQP
                     EMAIVRGSEGSGPYRAERR"
BASE COUNT          386 a          815 c          807 g          392 t
ORIGIN      
        1 tgaaccttca ccgcaagggc gagatggtga aggcggtcgt cctcgacgtc gacgtcgaga
       61 aggagcgcat ctcgctgggc atgaagcagc tcgagcgggg cggggtcgcc aagggcggaa
      121 cctcggccgc gggcggcgga gtgaacaaga acgagacggt gacggtgacc gttctcgagg
      181 tccgcgacgg cgggctcgag gtccaggtcg gcgacgacgg gcccacgggc ttcatcaagc
      241 gcatcgatct cggccgcgac cgcgacgagc agcggcccga gcgcttccag gtcggccaga
      301 agttcgacgc tctggtgatc ggcttcgacc gctccaagaa gcccaacttc tcgatcaagg
      361 cgcttcagat ccacgaggag aagcaggccg tcgcccagta cggctcctcg gactcgggag
      421 ccagccttgg cgatatcttg ggtgaagcgc tgaaaaacaa aggcaaggaa taatcccgga
      481 tcgggcgggc cgcggcgcgg tccgcccttt cgcccggtcg ccgatattgt gcggcaaggg
      541 tcgaattttc cttgtttttt ccaacctcag atattgcgct tcgggcaaat ttgagattta
      601 acctgggtta gcggtcaagc ttcgggggcg aagcgaggtc cgtgcaggcc gtttttctcc
      661 tggggggaag ctgtgatccg ttccgagctc gtgcagctgc tttgcgatga tcatcccgat
      721 cttaccggca aggagatcga gcgggtggtt tccgcctttt acgattcgat caccgaccag
      781 ctccagcagg gcggccgggt cgagcttcgc ggcttcggcg ccttctcgac ccgcgagcgg
      841 gacgcccgca agggccgcaa cccccgcacc ggcgactccg tcgacgtcga cgccaagcgc
      901 gtcccttact tcaagcccgg caaggaaatg cgcgagcgcc tcaacgtctg agcgggccgg
      961 cgccgtcatc ccgggcttga cccggatcca tggacagcgc cgcgactgaa ttcatcaatg
     1021 acttgagaca cgccgcccga ggaaaaggtg tcggcatggg ctttggacgc ggtcccgcgc
     1081 tttccctgag ttgccgctcg gcccggcacc gcttccgccg ccgctccgat catggcatgc
     1141 catggaggtg cggacgtggc ggaaccggta gacgccggag acttaaaatc tcctgcccct
     1201 cggggcgtgc gggttcgagt cccgccgtcc gcaccagagg caagccgttt tcatcgcccg
     1261 ccggcgcgat tggctcatct cggagaaacg aaacattcct cgccgctcgg gaagagtgat
     1321 ccggcttacc tgccttagcc gcggcggcca agtatatgtt cccagggcag gggcagccct
     1381 caggccgcct gctgcggcgg ctcggccggc accgcggtcc attcgcagcg gcgcaggctg
     1441 atcgggtcga cgaattggcc gcccatcctg tcgccgagct gccagcggac ctgggcgttg
     1501 actgccccta cccccggcac gtcgagcgag acgtagctgc cgatccggac gggctcgctg
     1561 cactcggcca tgaagccgct ggtcgagacg tcgcagaccg tcacctcgat gctgtaccag
     1621 ctctgggcgc gcagctgggt acggcccgcc actggggtcc ggcccgatcg gcgcggcttg
     1681 tactggttca tgcgcgcgat cctagcgcgg caaaggtgaa ggcgcggtaa aagcggcgac
     1741 gaggcgtcac tcaggcgaaa gccaggggcc cgccgggtga aggactcggc gcccctcctc
     1801 gccgaggtcc cggccttcgc cgggatgacg gtcgtcggcc gctgcgctat cgctgcctcg
     1861 atatggtgac tcgcttcctt ccggcgccgc ggctgctcgc ggcgacactc cttctcgccg
     1921 gctgcggcgg cgaggagacc gggccgatct cgatcagcgc catcggcggc caaccccgga
     1981 tcgccaatcc cagccgcgat cggctcgacc cgccctccgc gatcctgatc gaagccgccg
     2041 cccagggcct cgtccgcttc gacgccggcg gcgagatcga gcccgctctc gcgcagcgct
     2101 ggatcgtctc ggacgacggc cttcgctaca ccttccggct cggcgaggcg agctggggcg
     2161 ggcgccggat caccgccgag caggtcgtgg cgaggcttcg cgccgccgtt gcgccggcga
     2221 gccgcaatcc gctcaagcct gtcctcggag cggtcgacga gatcgttgcg atgaccgacg
     2281 acgtgctcga gatcagcctc aagtcgccgc ggcccaactt ccttcagctc ctcgcccaac
     2341 ccgaaatggc gatcgtccga ggcagcgagg gttcgggccc ctaccgggcg gagcggcggc
//
LOCUS       DASWNU010000575         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_411388, whole genome shotgun sequence.
ACCESSION   DASWNU010000575 DASWNU010000000
VERSION     DASWNU010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411388"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /gene="cpaB"
                     /locus_tag="VGB57_11395"
     CDS             <1..943
                     /gene="cpaB"
                     /locus_tag="VGB57_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0131997.1"
                     /translation="KPAVPAGPEVLVATRALPVGTIIDAEAFRFQRWPEGLVQPAYYI
                     KGKPGVRPADLVGTVVRNEITAGQPFTQGSMIKPGERGFLAAALGPGMRAVTVAVSAT
                     SGVAGFVFPGDRVDIVLTQEVAGGGDGLPLKVSETVMRNVRVLATDQRMDAKGEDGKA
                     VVQRFTMVTLEATPKLAEKIAVAQKIGDLSLSLRSIADNSAELERAIASGEVKVPQTD
                     DPKAERQMLLAVANQPTDGISTYTTGSDVSRFQRSTVPGSGKAKASPDDKIADALSAM
                     AYAAAGRAPPGTPSTPSGPQVRVSRGNSVTIVPVGAK"
     gene            945..2552
                     /locus_tag="VGB57_11400"
     CDS             945..2552
                     /locus_tag="VGB57_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEY0131998.1"
                     /translation="MTKLNIMRRATVGTAIFAVLTASVTPVMPSLALAAQPSGSYRPT
                     NKVLLSVGEGEVISLPRNVVDVWTSNPGVADVNVTSPRQLGLFGKGDGEATVIATAAD
                     GSVVWGAHVRVSQNISSVDQMLKAAMPEANISITHVGQIAVLNGTVASPADSAQAEAL
                     VRTMLNPGIDANAPGAILKIIPVNRLRSATPQQVMLQVRIAEVSRSLARDIGVNLASA
                     DSTGGFKFGIGQGRTITPKYTPGGPIGTGDTASEGATAVVTNSIGSTLFGAGKLLGVD
                     ILGALDLAETDGKVHTLAEPNLVALSGETASFLAGGEFPIPSSNGINGTAIEFKQYGV
                     SLAFTPYVLEGGRISMRVRPEVSELSDEGSIQINGFRVPSLTTRRVETTVELGSGQSF
                     MIGGLLRNSNSNSIEKAPILGNLPIIGALFRSTQFRRGETELVIVVTPYLVKPVNASQ
                     IALPTDGFRSSNTAERVMLGTSEYNKPVPRPGPTMAPPRTASPGMGGVASVPARQLPA
                     PAQPRQAEANAKATRPQTAAAAPGFSF"
     gene            2573..>3140
                     /locus_tag="VGB57_11405"
     CDS             2573..>3140
                     /locus_tag="VGB57_11405"
                     /inference="COORDINATES: protein motif:HMM:NF021025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HEY0131999.1"
                     /translation="MARTKSLAALVAIGAALTLPSVALAKKPPRERHGPERGLDSLNQ
                     PVVQRTDYLLDLADNGGLGDSDMGRLDAWFESLGLGYGDHVFVDRGTDAGRRDVARVA
                     AAYGLLMSPGAPILEGDVPMGAVRVIVSRTTASVPNCPNYDTERGPSSTSSNYGCSVN
                     SNLAAMIADPNDLVLGQTGSVYGDAHTST"
BASE COUNT          526 a         1175 c          989 g          450 t
ORIGIN      
        1 caagcccgcg gtgcccgcgg ggcccgaagt gctggtcgcg acccgcgccc tccccgtcgg
       61 caccatcatc gacgcggagg ccttccgctt ccagcgctgg cccgaaggcc tcgtccagcc
      121 cgcttactac atcaagggca agcccggcgt ccgcccggcc gacctggttg gaactgtggt
      181 ccgcaacgag atcacggccg gccagccctt cacccagggt tcgatgatca agccgggcga
      241 gcgcggcttc ctcgccgcgg cgctcgggcc ggggatgcgc gccgtcaccg tcgccgtctc
      301 cgcgaccagc ggagtcgccg gcttcgtctt cccgggcgac cgggtcgaca tagtcctcac
      361 ccaggaagtg gccggcggcg gcgacggact tccgctcaag gtctccgaga ccgtgatgcg
      421 caacgtccgg gtgctcgcca ccgaccagcg catggatgcg aagggcgagg acggcaaggc
      481 ggtcgtccag cgcttcacca tggtcacgct cgaggcgacc cccaagctcg ccgagaagat
      541 cgcggtcgcc cagaagatcg gggacctgtc cctttcgctt cgctcgatcg ccgacaattc
      601 ggccgagctc gagcgcgcca tcgcctcggg cgaggtgaag gtgccgcaga ccgacgatcc
      661 caaagcggag cgccagatgc tgctcgccgt cgccaaccag ccgacggacg gcatttccac
      721 ctatacgacc gggtccgatg tctcgcgctt ccagcgcagc accgtccccg gcagcggcaa
      781 agcaaaggcg tcacccgacg acaagatcgc ggacgccctc agcgccatgg cctacgccgc
      841 cgcgggccga gcgcctcccg gtaccccgtc gactccctcg ggtcctcagg tccgggtctc
      901 ccgcggcaat agtgtcacca tcgttccggt cggagccaaa taagatgaca aagctgaaca
      961 tcatgcgccg agcgacggtc ggaaccgcga tcttcgcggt cctcaccgcc agcgtcactc
     1021 cggtgatgcc gagcctggcc ctggccgcgc agccctcggg cagctaccgg ccgaccaaca
     1081 aggtgctgct gtccgtgggc gaaggcgaag tcatatcgct gcctcggaac gtggtcgacg
     1141 tctggacgtc caacccgggc gtggccgacg tcaacgtcac cagcccgcgc cagctcggcc
     1201 tcttcggcaa gggcgacggc gaggccacgg tgatcgccac ggccgccgac ggctcggtcg
     1261 tctggggcgc ccacgtccgg gtcagccaga atatcagctc ggtcgaccag atgctcaagg
     1321 ccgcgatgcc ggaagccaac atctcgatca cccatgtcgg ccagatcgcc gtgctgaacg
     1381 ggaccgtcgc ctcccccgcc gacagcgccc aggccgaggc gctggtgcgg acgatgctga
     1441 accccggcat cgacgccaac gcgcccggcg cgatcctgaa gatcattccg gtcaaccgcc
     1501 tcaggtcggc gactccgcaa caggtgatgc tgcaggtcag gatcgccgag gtgagccgct
     1561 cgctcgcccg cgacatcgga gtcaatctgg cctcggccga ttcgaccggc ggcttcaagt
     1621 tcggcatcgg ccagggccgc accatcactc cgaaatacac cccgggcgga cccatcggaa
     1681 ccggggatac ggcgtccgag ggcgccacgg ccgtcgtcac gaattccatc ggctccaccc
     1741 tcttcggggc cggcaagctg ctcggcgtcg acatccttgg ggcgctggac ctagccgaaa
     1801 ccgacggcaa ggtgcacacg ctcgccgagc cgaacctcgt cgctctttcg ggcgagaccg
     1861 ccagcttcct cgccggcggc gagttcccga tcccgtcgtc gaacggaatc aacggcacgg
     1921 cgatcgagtt caagcaatat ggcgtcagcc tcgccttcac gccctacgtt ctcgaaggcg
     1981 gccggatctc gatgcgggtt cgcccggaag tgtccgagct ttcggacgag ggctcgatcc
     2041 agatcaacgg cttccgggtc ccctcgctga ccacgcgccg ggtcgagacc accgtcgagc
     2101 tgggctccgg ccagagcttc atgatcggcg gactgcttcg caacagcaac agcaacagca
     2161 tcgagaaagc gccgatcctg ggcaatttgc cgatcatcgg cgccctgttc cgctcgaccc
     2221 agttccgccg cggcgaaacc gagctggtga tcgtggtcac gccctatctg gtgaagccgg
     2281 tgaatgcgag ccagatcgcg cttccgaccg acggcttcag gagctccaac acggcggagc
     2341 gggtgatgct tgggaccagc gaatataaca agcccgtccc ccgtccgggg ccgaccatgg
     2401 ctcctccgcg caccgcgagc ccgggcatgg gcggcgtggc gagcgtgccc gcccggcagc
     2461 ttccggcccc ggcgcagccg cgccaggccg aggcgaacgc caaggcgacc cgcccgcaaa
     2521 ccgccgccgc cgcccccggc ttcagcttct gaccgatcga aaggacgtga tcatggcacg
     2581 caccaaatct ctcgccgccc tcgtcgcgat cggcgccgcc ctcaccctcc cttccgtcgc
     2641 actggccaag aagccgccgc gcgagcggca cgggccggaa cgcggtctcg actccctcaa
     2701 ccagccggtc gtccagcgca ccgactatct gctcgacctc gccgacaacg gcgggctcgg
     2761 cgactccgac atgggccggc tcgacgcctg gttcgaatcg ctcggcctgg gctatggcga
     2821 ccatgtcttc gtcgaccgcg gcaccgatgc cggtcgccgg gacgtggcca gggtcgcggc
     2881 cgcctacggc ctgctgatga gccccggcgc tccgatcctg gaaggcgacg tgccgatggg
     2941 cgcggtccgg gtgatcgtca gccgaaccac ggcgagcgtt cccaactgcc ccaattacga
     3001 cacggagcgc ggcccgagct cgacgtcttc caattacggc tgctcggtga actccaacct
     3061 cgccgcgatg atcgccgacc ccaacgacct ggtcctcggc cagaccggca gcgtctacgg
     3121 cgacgcccac acctcgacca
//
LOCUS       DASWNU010000576         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_255752, whole genome shotgun sequence.
ACCESSION   DASWNU010000576 DASWNU010000000
VERSION     DASWNU010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255752"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VGB57_11410"
     CDS             <1..1004
                     /locus_tag="VGB57_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY0132000.1"
                     /translation="AEEVPAVAAKGINPVQHSEAAPQPKADPGDRVKASPLARRLAEQ
                     RGIDLSSLAGSGPGGRIVKADVDGAAGRPAAAPAQAQAPAQAEAPAQAPAAPAPTYGV
                     PEIPHEVAKLSNMRKTIARRLTESKQNVPHIYLTVDIRLDALLKLRGELNASLEPRGV
                     KLSVNDLMIKALAAALIEVPECNVQYTGSELITFKRADISVAVSIPGGLITPVVTEAD
                     SRSLSSIATAMKDLAARARDNKLKPEEYQGGTASLSNMGMYGIRQFEAVINPPQGMIM
                     AIGAGEKRPYVVDDSLQIATVMSATGSFDHRAIDGADGARLMQAFKRLVEAPLGMLA"
     gene            1006..1215
                     /locus_tag="VGB57_11415"
     CDS             1006..1215
                     /locus_tag="VGB57_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132001.1"
                     /translation="MALGKAVLGGLVASIAIPYVLRGHSGQLGEWTRRGLIDFEVAGH
                     QIYWSLPLFAGVTLIAWLFLSWADK"
     gene            1232..1666
                     /locus_tag="VGB57_11420"
     CDS             1232..1666
                     /locus_tag="VGB57_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132002.1"
                     /translation="MTPAERGVRLLAVAGAAALVAGSVTGTLLTLEDPVWPERGGLEG
                     LLQACALFTAYAFAVALAVGALLAVTLSPLMEKAGRPGPLAHLLASLPLGLGVHLLVD
                     VAIGERVDSAGDWLGGTVAGGAAGPLWWFLVQRHLKKASRNG"
     gene            1659..3041
                     /gene="lpdA"
                     /locus_tag="VGB57_11425"
     CDS             1659..3041
                     /gene="lpdA"
                     /locus_tag="VGB57_11425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0132003.1"
                     /translation="MADTYDLIVLGSGPGGYVAAIRASQLGLKVAIVEREKLGGICLN
                     WGCIPTKALLRTAEIYHYMTHASAYGLSAEKPAFDLAKIVDRSRKVAGQLNAGVKGLM
                     KKNKVTVVEGDGALKGKGTLSVTKDGKTTDLHGKQILVATGARARDLPFAKADGERIW
                     TYRHAMVPKVMPTRLLVIGSGAIGVEFASFYSDMGAEVTIVEMLDRILPVEDEDVSAF
                     VHKALVKQGMKLHVSSGVEKLETGPDGVKATLKGGDVQEFSHAIVAIGIVPNTEAIGL
                     EALGVKTERGHIVVDGYGRTNVEGVYAIGDDTGPPWLAHKASHEGVVCVEAIAGLKPH
                     PFETWNIPGCTYSRPQVASVGFTEAKAKELGRKVKVGKFPFIGNGKAIALGEAEGFVK
                     TVFDSETGELLGAHMVGAEVTELIQGYAVARQLETTEEDLMHTIFPHPTLSEMMHESV
                     LDAYGRAVHY"
     gene            3099..3386
                     /locus_tag="VGB57_11430"
     CDS             3099..3386
                     /locus_tag="VGB57_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0132004.1"
                     /translation="MPRIEKTPAFITWQRKLRDERAAAKIAIAITRLGFGLGDVVSVG
                     DGVSEVRIAYGPGYRLYFTRRGEELIILLCGGDKSSQDRDIRKAKETAAAL"
     gene            3432..3782
                     /locus_tag="VGB57_11435"
     CDS             3432..3782
                     /locus_tag="VGB57_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEY0132005.1"
                     /translation="METTPTRAATPYDASEFLGSSEAQAELLNDALSSGNPAYVAHAL
                     GIIAKAIGMSEIARRTGIKRQSLYSALDEEGNPTLETFFSVLQALGIVLQAEGAAAAA
                     GAEVEKRERVAAAG"
     gene            complement(3985..>4202)
                     /locus_tag="VGB57_11440"
     CDS             complement(3985..>4202)
                     /locus_tag="VGB57_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132006.1"
                     /translation="ERCAPYVDRYGRMRVDDVRRHGRNGWRVYGTVGGAAIADSNGWR
                     RGYAPRAFTCTVREDGRVKLKTSKLRR"
BASE COUNT          758 a         1390 c         1390 g          664 t
ORIGIN      
        1 cggccgagga ggttccggcg gtcgcggcca agggcatcaa tccggtccag cacagcgaag
       61 ccgctccgca acccaaggcc gatccgggcg accgggtgaa ggcgagcccg ctcgcccgcc
      121 ggctggccga gcagcgcggg atcgaccttt cgagcctcgc cggctccggg ccgggcgggc
      181 ggatcgtcaa ggccgacgtc gacggtgcgg ccggcaggcc cgcggccgct ccggcacagg
      241 cccaggcgcc cgcacaggcc gaggcgcccg ctcaggctcc ggccgcgccc gcgccgacct
      301 acggcgtgcc ggagatcccg cacgaggtcg ccaagctctc gaacatgcgc aagacgatcg
      361 cccggcgcct gaccgaatcg aagcagaacg tcccccacat ctatctgact gtcgacatcc
      421 ggctcgacgc cctcctcaag cttcgcggcg agctcaacgc ctcgctcgag ccgcgcgggg
      481 tcaagctttc ggtcaacgat ttgatgatca aggcgctcgc cgcggcgctg atcgaagttc
      541 ccgagtgcaa cgtccaatat accggcagcg aactgatcac cttcaagcgc gccgacattt
      601 cggtcgcggt gtcgatcccg ggcggcctga tcacccccgt cgtcaccgag gcggattcgc
      661 gcagcctctc ctcgatcgcc accgcgatga aggacctcgc cgcccgagcc cgcgacaaca
      721 agctgaagcc cgaggaatat cagggcggga cggcgagcct ttcgaacatg ggcatgtacg
      781 ggatcaggca gttcgaggcc gtcatcaatc cgccgcaggg gatgatcatg gccatcggcg
      841 ccggcgaaaa gaggccctat gtggtcgacg acagcctcca gatcgcgacg gtgatgagcg
      901 cgaccggaag cttcgaccac cgcgccatcg acggcgccga cggcgcccgg ctgatgcagg
      961 cgttcaagcg gctggtcgag gctccgctcg gaatgctggc ctgacatggc gcttggcaaa
     1021 gccgtcctcg gcggactcgt cgcctccatc gccatcccct acgtgctgcg cggccacagc
     1081 ggccagctcg gcgaatggac ccgccgcggc ctcatcgact tcgaggtcgc cggtcaccag
     1141 atctactggt ccctccccct cttcgccgga gtgaccctga tcgcctggct gttcctgtca
     1201 tgggcggaca agtgaggcgg gggcggtccg cttgacgccc gccgagcgag gcgttcggct
     1261 cctcgccgtc gccggagcgg cggcgctggt cgcgggctcc gtcaccggca ccctgctgac
     1321 gctcgaggac ccggtctggc ccgagcgcgg cggcctcgaa ggcctgctcc aggcctgcgc
     1381 cctgttcacc gcctacgcct tcgcggtcgc gcttgccgtg ggcgcgcttc tcgccgtcac
     1441 cctctcgccg ctgatggaga aagcgggccg cccggggcct ctcgcccacc ttctcgccag
     1501 tcttcccctc ggcctcggag tgcatctgct cgtagacgtc gcgatcggcg agcgtgtcga
     1561 ctccgccggg gattggctgg gcggcaccgt ggccggaggc gccgccgggc cgttatggtg
     1621 gttcctcgtc cagcgtcatc tgaagaaagc atcccgaaat ggctgacact tacgatctca
     1681 tcgtcctcgg ctccgggccc ggcggctatg tcgccgcgat ccgggcgagc cagctcggcc
     1741 tcaaggtcgc gatcgtcgag cgcgagaaat tgggcgggat ctgcctcaac tgggggtgca
     1801 tcccgaccaa ggcgctgctt cgcaccgccg aaatttacca ttatatgacc cacgcctccg
     1861 cttacggcct cagcgcggag aagcccgcct tcgacctggc gaagatcgtc gaccgctcgc
     1921 gcaaggtcgc cggccagctc aacgccggcg tcaagggcct gatgaagaag aacaaggtga
     1981 cggtggtcga gggcgacggg gcgctcaagg gcaaggggac gctgagcgtc accaaggacg
     2041 gcaagacgac cgatctccat ggcaagcaga tcctcgtcgc caccggcgcc cgagcccgcg
     2101 acctgccctt cgccaaggcg gacggggagc ggatctggac ctaccggcac gccatggtgc
     2161 ccaaggtcat gccgaccagg ctgctggtga tcggatcggg ggcgataggg gtcgagttcg
     2221 cgagcttcta ttcggacatg ggcgcggagg tgacgatcgt cgagatgctc gaccgcatcc
     2281 ttcccgtcga ggacgaggac gtctccgcct tcgtccacaa ggcgctggtg aagcagggga
     2341 tgaagctcca cgtctcgtcc ggggtggaga agcttgagac cggcccggac ggggtgaagg
     2401 cgaccctcaa gggcggcgac gtccaggaat tctcccacgc catcgtcgcc atcggcatcg
     2461 ttccgaacac ggaggctatc gggctcgagg ccctgggcgt gaagaccgag cgcggccata
     2521 tcgtcgtcga cggctatggc cggaccaatg tcgagggcgt ctacgcgatc ggcgacgaca
     2581 ccggcccgcc ctggctggcg cacaaggcga gccacgaggg cgtcgtctgc gtcgaggcga
     2641 tcgctggctt gaagccccat ccgttcgaga cctggaacat cccgggctgc acttactcgc
     2701 gcccgcaggt ggcgagcgtc ggcttcaccg aggccaaggc gaaggagctc ggccgcaagg
     2761 tcaaggtcgg caaattcccc ttcatcggca acggcaaggc gatcgcgctc ggcgaggcgg
     2821 aaggcttcgt caagacggtg ttcgactcgg agaccgggga gctgctgggc gcccatatgg
     2881 tgggggcgga ggtgacggag ctgatccagg gctatgcggt ggcccggcag ctcgagacca
     2941 ccgaggagga tctgatgcac acgatcttcc cgcatccaac gctgagcgag atgatgcacg
     3001 agagcgttct cgatgcttat gggcgggcgg ttcactactg atctgcgaca ctgtcagttt
     3061 tatttgacag tagaggttgt gtcatataaa actgacacat gcccaggatc gaaaagaccc
     3121 cagctttcat cacttggcag cgcaagctgc gtgatgaaag ggcggcggca aagatcgcga
     3181 tcgcgatcac ccgcctcggt ttcgggctcg gcgacgtcgt cagtgtagga gatggagtca
     3241 gcgaagttcg gatcgcatac ggacccggct accgcctcta tttcactcga cgcggagaag
     3301 aactgatcat cctgctttgc ggtggggaca aatcgtccca ggatcgggat atcagaaaag
     3361 cgaaggagac ggccgccgcc ctatgaaatt gcggcacgga aaaacgtaat gccaaggaag
     3421 gcaatctaat catggaaacg acccctaccc gggccgccac cccttatgac gcgtccgagt
     3481 tcctcggcag cagcgaggcg caggcggaac tactcaacga cgcgctctca tccggcaatc
     3541 ctgcttatgt cgctcatgca cttggcataa ttgccaaggc aatcggtatg tccgagatcg
     3601 ctcgcaggac cgggatcaag cgccaatcgc tttattcagc gctcgatgaa gagggaaatc
     3661 caaccctcga gaccttcttc tcagtgcttc aggcgctagg gattgtgctg caggcggaag
     3721 gcgcggcagc cgcggccgga gcggaggtgg aaaagcgcga aagagtagcg gccgccggct
     3781 gagcatcaac aggaccgtac acgcaggcga gggtaccgag ctgatccagg ctatgcggtg
     3841 gcgaggcagc tcgagaccac cgaggaggac cttcatgcac acgatcttcc cgcatccgac
     3901 gctgagcgag atgatgcact gccggcggga ctggctccgg ccttcgccgg aaacgactca
     3961 gcttcgagcg tacagactcc aacgtcagcg ccgcagcttg ctggtcttga gcttgacccg
     4021 gccgtcttct cggacggtgc aggtgaaggc tcggggggcg tagccccggc gccagccgtt
     4081 cgagtcggcg atcgcggcgc ctccgacggt gccgtagacc cgccagccgt tccggccgtg
     4141 gcgccgtacg tcgtcgaccc tcatccggcc gtagcgatcg acataagggg cgcagcgctc
     4201 ga
//
LOCUS       DASWNU010000577         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_176902, whole genome shotgun sequence.
ACCESSION   DASWNU010000577 DASWNU010000000
VERSION     DASWNU010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176902"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VGB57_11445"
     CDS             complement(<1..754)
                     /locus_tag="VGB57_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY0132007.1"
                     /translation="MAQQVASAPLPPAPGPAELATSALAQSPGQRPFRSPRHTYGALD
                     LGTNNCRLLIARPAEDGFVVIDAFSRVVRLGEGLAGSGRIADAAMDRAVAALSVCAEK
                     LKRRHVSLARSVATEACRRAVNGRQFVERVRQETGICLEIIAPEEEARLAMLGCHRLL
                     EPGDAPALIFDIGGGSTELILIDTDQEGTPKIKCWWSAPWGVVSLTESEGRDFADADE
                     RLAAYGRMKERVRHAFRRFAELLPPREGNIRLL"
     gene            1038..1544
                     /locus_tag="VGB57_11450"
     CDS             1038..1544
                     /locus_tag="VGB57_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HEY0132008.1"
                     /translation="MNQVTAFLGDRIVARGEREAVTRELERLYPADLAVLRVFEDGSG
                     RVVDLDYWDAAASAPPPPRRRGRPSLGVAAREVTLLPRHWDWLSRQPGGASAAIRRLV
                     EEASRKAPGPAEKRDSAYRFLSDMCGDRPGYEEALRALYRGEEARFHILIAGWPQQIR
                     GYVKELLA"
     gene            complement(1673..2296)
                     /locus_tag="VGB57_11455"
     CDS             complement(1673..2296)
                     /locus_tag="VGB57_11455"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY0132009.1"
                     /translation="MDSTDAADLIAEIHEDRAENQAAESFRNRAALMIAVLAAVLAVG
                     GLGGGNATDDMIENNIKASDTWAFYQAKNVRQTMYKIEAEKLKTSLAAGIVPPAERPS
                     AEARLRDYESTIARYDSEPDPNDPSKGEGKKELSATAKGYEAAFEQAARRDNNFDFAE
                     VLLQLALVLGSVAILAVNRWILITSAALGALGALLTLNGFFLLAPLP"
BASE COUNT          352 a          774 c          841 g          392 t
ORIGIN      
        1 cgagcagccg gatattgccc tcgcgcggcg gaagaagctc ggcgaatcgg cggaaggcgt
       61 ggcggacccg ttccttcatc cggccgtagg ctgcgagccg ctcgtcggcg tcggcgaagt
      121 cgcggccttc gctctcggtg agggagacta cgccccaggg cgcgctccac cagcatttga
      181 tcttcggagt gccctcctgg tcggtgtcga taaggatgag ctcggtcgag ccgccgccga
      241 tgtcgaagat gagcgcgggc gcgtcgccgg gctcgagcag ccggtggcac cccagcatcg
      301 cgagccgggc ttcctcctcc ggggcgatga tctccaggca gatgccggtc tcctgccgaa
      361 cccgttcgac gaactggcgg ccgttgacgg ctcggcggca ggcctcggtg gcgaccgagc
      421 gggcgaggga gacatggcgt cttttcagct tctcggcgca gaccgagagc gcggccaccg
      481 cccggtccat cgccgcgtcg gcgatccggc cgcttccggc cagcccctcg ccgagccgga
      541 cgactcgcga gaaggcgtcg atgacgacga aaccgtcttc cgccggtcgg gcgatcagga
      601 ggcggcaatt gttggtgccg agatccagcg cgccataagt gtgccggggg gagcggaaag
      661 gccgctgccc gggcgactgg gcgagcgcag acgtggcgag ttcagccggg ccgggtgccg
      721 gcgggagcgg agccgaagca acctgctgcg ccatagcgga aaaactcttt ttttctcgtt
      781 ccggcaggcg gggccgccgg tgacttggca ccttcgtacc ccgccccgcc cccgtcggca
      841 agggagtggg gcgcccgggg tgggacaagg gcgtgagaca ccccgtcatc ccggcgaaag
      901 ccgggacctc cggtcggtaa gcctcggcgg gcctccccga ggtcccggct ttcgccggga
      961 tgacgagaat aatttcctcc gggtatattt gacacgggat tatacccgga tataaaagcc
     1021 gccgaaggag cgagacgatg aatcaggtga ccgcctttct gggcgacagg atcgtggcgc
     1081 gcggcgagcg cgaagcggtg acccgcgagc tcgagcggct ctatcccgcc gaccttgccg
     1141 tccttcgggt gttcgaggac ggcagcggcc gggtcgtcga cctcgattat tgggacgccg
     1201 ccgcctccgc gccgccgccc ccgcgccgga ggggccgccc gagcctcgga gtcgcggcgc
     1261 gggaggtgac cctgcttccg cgccactggg actggctgtc gcgccagccc ggcggagcct
     1321 ccgccgcgat ccgccggctg gtcgaggaag cgagccgcaa ggcgcccggt ccggccgaga
     1381 agcgcgattc ggcctatcgc ttcctgtccg acatgtgcgg cgaccgcccc ggctacgagg
     1441 aggcgctgcg cgcgctctac cggggcgagg aggcgcgatt ccacattctg atcgccggct
     1501 ggccccagca gatccgaggc tatgtgaagg agctgctcgc ttaattcctc ccctgccttt
     1561 gcaggggagg ggaaccgcgc gaagcgcggt ggaggggtct tgctgagtcc gaagaacccc
     1621 tccaccagcc ttcggctggt ccccctcccc ggcaaatgcc ggggaggatt ttctagggca
     1681 gcggcgccag caggaagaag ccgttcaggg tgagcagagc gccgagcgcc ccgagagcgg
     1741 cggaggtgat caggatccag cggttgaccg cgaggatcgc gaccgatccg aggacgagcg
     1801 cgagctggag gagcacttcg gcgaaatcga aattattgtc gcggcgggcc gcctgctcga
     1861 aggccgcctc atagcctttc gcagtcgcgg aaagctcctt cttgccttcg cctttggagg
     1921 gatcgttcgg gtcgggctcg ctgtcgtagc gggcgatggt ggactcataa tccctgagcc
     1981 gggcctcggc cgaggggcgc tcggcgggag ggacgatccc ggccgcgagc gacgtcttca
     2041 gcttctccgc ctcgatcttg tacatggtct ggcggacgtt cttggcctgg tagaaggccc
     2101 aggtgtcgct cgccttgata ttgttctcga tcatgtcgtc ggtggcgttg ccgccgccga
     2161 gcccgccgac ggcgagcacc gcggccagaa ccgcgatcat cagagccgcg cggttgcgga
     2221 agctttcggc ggcctggttt tccgcccggt cctcgtggat ttccgcgatc aggtctgcgg
     2281 cgtcggtcga atccattctt cgtttccccc cggaatttcg cgttggctcg caaattttcc
     2341 ggcgcaagtc gagccgctt
//
LOCUS       DASWNU010000578        10593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_74162, whole genome shotgun sequence.
ACCESSION   DASWNU010000578 DASWNU010000000
VERSION     DASWNU010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74162"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..977
                     /locus_tag="VGB57_11460"
     CDS             192..977
                     /locus_tag="VGB57_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132010.1"
                     /translation="MSIQLDTPDASMFEPPAEAVDFYVESLKLLKESEIPFLLSGTFA
                     LSCFTGITRPTKDLDVFCKPSDAPKILSFFKGRGYEIQMEDERWIGKVWRGEHFFDVI
                     FNISSASIPITDDWFQEVYEAEVYGTNVRVTPPTHFILSKLFLQTRYRYDGADIVHTI
                     LIKHEEIDWRWLLSNMELYWEVLLINILNFRFVYPTERDLIPRWLYEELLERLRAQDQ
                     MPPASIKICRGRLISPTDYVVDVSEWGFADVVGKGVDEKHEPH"
     gene            964..1689
                     /locus_tag="VGB57_11465"
     CDS             964..1689
                     /locus_tag="VGB57_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0132011.1"
                     /translation="MSPTETLIVAAIGDLHVTETDQNRYRALFEEISEAADVLVLAGD
                     LTNFGKTAEAENLAEDLRGCTIPVVAVLGNHDYEEGQPDEVARILQSAGVTMLGEQAT
                     VIEGVGFAGAKGFIGGFGRGELGAFGEDAIKLFVDEARNEARLLENALRSLRTERVVA
                     VLHYAPIPETVEGEPLEIYPFLGSSRLAHAIDRFDNVKAVVHGHAHRGAYSGHTPGGV
                     PVFNVAQFVVEPAFGRPYALLEI"
     gene            complement(1791..2666)
                     /locus_tag="VGB57_11470"
     CDS             complement(1791..2666)
                     /locus_tag="VGB57_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132012.1"
                     /translation="MDYEPGRVVTAKPKRPLWTLLALPLVAFLAGLAAMGWLLANWSN
                     AATFLGVAPAPAPAVEPAPQPMTVDREPAVGPPPEPQAGERLVIDPETARRVNRLEER
                     LALLDLQSRTAVGNADRAEGLLVAFAARRAIDRGVALGYIETLLRQRFAGTEPQAVAT
                     VLTVARSPVTVQSLQKQFQEVAPHLTGGGQGQTWWAAFKTEMAGLVTIRRENTPSTQP
                     AERLRRAGQSLEAGQVEVALLEVLRLPGHERADPWIANARRYVAAHRALDRLESAALL
                     EPRAPPAAAVGVRPN"
     gene            complement(2656..3330)
                     /locus_tag="VGB57_11475"
     CDS             complement(2656..3330)
                     /locus_tag="VGB57_11475"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0132013.1"
                     /translation="MKLFILRPEPGASETAARARAMGLDPLVAPLFAIRSVEGPPVDA
                     ARYEAVLLTSANGARHAPAGLTALPCFAVGESTAAAARSAGFAKVRAGPCDGAAAAAM
                     MAAAGVKKALHPCGREHLPVDAAGVAIDRRIVYSAEPVPHGRFEGPAVAMIHSARAGT
                     RFAELTDDRSAIAIAAISPGAAAAAGEGWAAKAVAAAPRDQALLELAVKLCNHGVDVV
                     RDGNGL"
     gene            complement(3327..4238)
                     /gene="hemC"
                     /locus_tag="VGB57_11480"
     CDS             complement(3327..4238)
                     /gene="hemC"
                     /locus_tag="VGB57_11480"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0132014.1"
                     /translation="MQSVLRLGTRGSPLALVQARLVATALRDAHGWGPELIEIEPIAT
                     SGDQLRDRSISEMGGKALWTKELDSALIEGRIDLAVHSMKDVETIRPPELALVAMLER
                     ADVCDRLVGAPSLALLPEGARVGTSSPRRAAQLLARRPDLVIVPFRGNVETRLRKIQR
                     GEVDATLLAAAGLDRLGRSDCGILLDDFLPAPAQGAIGVEVRSSDRRVRTLVAAIDHR
                     ATHLCVAAERRLLEGLGGSCRSPVAALATLEGDQIRLRAEILTEDGMEVEKCDRQFRS
                     GDDKAPLELAREMLDRAPSRLRALFAL"
     gene            4283..5317
                     /gene="tsaD"
                     /locus_tag="VGB57_11485"
     CDS             4283..5317
                     /gene="tsaD"
                     /locus_tag="VGB57_11485"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0132015.1"
                     /translation="MSLILGIESSCDETAAALVTSDRHILAHCLAGQESAHSPFGGVV
                     PEIAARAHVEILPGLIEQALAESGVALAEVDAIAATAGPGLVGGVMVGLVAAKGLALA
                     TGKPLVAVNHLEGHALSPMLGDPALAFPYLLLLVSGGHCQLLHVEGLGRYRRLATTID
                     DAAGEAFDKTAKLLGLGFPGGPAVERAALAGDPAAVPLPRPLLGSAEPHFSFAGLKSA
                     VLRARDSGLHATGDIAASFQQAVVDSLVDRTRRALERVPEATALVVAGGVAANESVRS
                     ALTRLADAHGLPFVAPPLWLCTDNAAMIAWAGALRFDAGLTDPLDAPARARWPLDPDA
                     EKVRGAGVKA"
     gene            5314..6303
                     /locus_tag="VGB57_11490"
     CDS             5314..6303
                     /locus_tag="VGB57_11490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY0132016.1"
                     /translation="MSGRPIGVLGGGAWGTALAQIMASDGTPVRLWAREADVVEAINT
                     RRENPLFLPGQKLSAAIRATSDLTWVAEVSPVLVVTPAQHMRSVLSDLPAHAETLILC
                     SKGMEAESLRLMHEVAREEHPGSPVAVLSGPTFAHEVAAGLPTAVTLACEDLDFARDL
                     AARVARPSLRPYAASDIVGAEIGGAVKNVLAIACGVVEGRRLGLNARSALISRGFAEM
                     TRFGLARGARAETLSGLSGLGDLVLTCSSVSSRNFSLGKGLGEGRPAAELLADRRTVA
                     EGAHTAPVLAKAAREAGVDMPIVSAVCALLAGEAGVDEVVERLLARPLRSEGA"
     gene            complement(6401..6595)
                     /locus_tag="VGB57_11495"
     CDS             complement(6401..6595)
                     /locus_tag="VGB57_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEY0132017.1"
                     /translation="MREPSSIGRAGRRPGHVKPPAHLSPSPPVPEPGPAAEGEEHGGP
                     KGEEPTRYGDWEKKGICWDF"
     gene            6633..7556
                     /gene="htpX"
                     /locus_tag="VGB57_11500"
     CDS             6633..7556
                     /gene="htpX"
                     /locus_tag="VGB57_11500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY0132018.1"
                     /translation="MNHFRTLILLAAMTALFMGLGFTLGGPRGAFLALIVAAAMNLIT
                     YWNADRIVLSMHNAKEVTVRDCPQLVGIVHDLAARAGLPNPRVYLVDSPHPNAFATGR
                     DPAHAAVAATTGLLGLLTKEEVAAVMAHELGHVRNRDTLVMTMTATIAGAISMLANFG
                     FFFRGDGERSNPLAMIAAVLLAPFAAMLVQLAISRTREYGADRAGAEICGDPRALASA
                     LAKLHQGAARVPSPVAMRNPAAASLYIVPSGTGRDSLFSTHPDTGNRIAALQAMASEF
                     APPLRAPAATAVPRTRRSGASALNPLGRRSR"
     gene            7553..8812
                     /locus_tag="VGB57_11505"
     CDS             7553..8812
                     /locus_tag="VGB57_11505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY0132019.1"
                     /translation="MNRPSDPAGVPARRAALSLLDAVLRKGLPLEAALDTAARGLAPN
                     DRGLAHAIAAETLRRLGDLDALIDGATRNRLPDDAKARFALRIALVQALALDTPPHAA
                     ISTVLPLVDGGPRKLVHGVFGTLMRQQARLPALPSLPAAVEARWTRNWGEAVAIEAAR
                     LLAAPPPLDLVFAAGTQPPAEMEGVSLMPGHLRLPARAAVAGLTGYGEGGWWVQDIAA
                     SLPARLLGSGEGRTALDLCAAPGGKTMQLASAGFEVTAVDSSESRLARLSENLARTNL
                     SAKVIAADILRWSPPDKVDAILLDAPCSATGIYRRHPDVLHRVRPRAIAELAEGQKAM
                     LARAAGWLKPGGTLVYSVCSLEPEEGEAVVEAFLADRRDFEAAHIEAGTLPAEIPVEG
                     NRVRILPGLFAEAGGADGFFIARFRRA"
     gene            complement(8901..9182)
                     /locus_tag="VGB57_11510"
     CDS             complement(8901..9182)
                     /locus_tag="VGB57_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132020.1"
                     /translation="MLNGMKRLFATFAASTLLAASALVATPASAQTGGGGGPDRCYAV
                     NSPYDCCEYIEEWGGWYRFYVWGMEPCNAYWAPVEPDGPSQEPPESPGQ"
     gene            complement(9310..9570)
                     /locus_tag="VGB57_11515"
     CDS             complement(9310..9570)
                     /locus_tag="VGB57_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132021.1"
                     /translation="MTNAIKRMSATCAAMVMAASLGATPAAAQTGSGTGNPDDPVYCT
                     QTLQGILYCCRLYDPCQQIWYDGARDDRRPEEPLKPSESPEE"
     gene            complement(9741..10001)
                     /locus_tag="VGB57_11520"
     CDS             complement(9741..10001)
                     /locus_tag="VGB57_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132022.1"
                     /translation="MRGTFAASILLAASLAAAPASAAKKPPPPPPPVEDPDDPWAGCT
                     PYGSYDYFKDCCGYPELVVPCESPASDPVIRPEEASAESSGQ"
     gene            complement(10139..10417)
                     /locus_tag="VGB57_11525"
     CDS             complement(10139..10417)
                     /locus_tag="VGB57_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132023.1"
                     /translation="MSNTRKRLSGAFAASVLMAASLAAAPAMAKKPPPPPVEDPDDPW
                     YGCTPWGPVIYGAECCDYPELIIPCQGPNPPVDPIIIPLEAKSEAAAG"
BASE COUNT         1545 a         3861 c         3549 g         1638 t
ORIGIN      
        1 aggacgcttg gtagcacggc ttcgagcggt atcctaatcc aagataggtc cataacgaat
       61 gcgattgggc gcgggacccc aggcaccctg gttgcgggcg gtgcggaacg catcgccgcc
      121 cgccccgttc tcccggcgat ccggccaatg cggcgctcct gccgcgatcc ggaggctgaa
      181 cgcgcgggga catgagcatt caactcgaca cgccagacgc ctcgatgttc gagccgcccg
      241 cggaagcggt cgacttctac gtcgagagcc tgaagctgct caaggaatcc gagataccgt
      301 tccttctgtc gggaaccttc gctttgtcct gcttcaccgg gatcacccgc ccgaccaagg
      361 atctggacgt cttctgcaag ccgagcgatg cgcccaagat cctcagcttc ttcaagggaa
      421 gaggctatga gatccagatg gaggacgagc gctggatagg gaaggtctgg cgcggcgaac
      481 atttcttcga cgtcatcttc aatatctcct cggcttccat cccaatcacc gacgactggt
      541 tccaggaggt ctatgaggcg gaggtctacg gcaccaacgt gcgggtcacc ccgcccaccc
      601 atttcatcct ctcgaagctc ttcctgcaga cccgctaccg ctacgacggc gccgacatcg
      661 tccacaccat cctgatcaag catgaggaga tcgactggcg gtggctgctc tccaacatgg
      721 agctctactg ggaggtgctg ctcatcaaca tcctcaattt ccgcttcgtc taccccacgg
      781 agcgcgatct gatcccgcgc tggctgtacg aggagctgct cgagcgcctc cgggcgcagg
      841 accagatgcc gccggcgagc atcaagatat gccgcggccg gctgatcagc ccgaccgact
      901 atgtggtcga cgtcagcgaa tggggcttcg ccgacgtggt ggggaaggga gtggacgaaa
      961 agcatgagcc ccactgagac gctcattgtc gcggcgatcg gcgatctcca cgtcaccgag
     1021 accgatcaga accgctatcg agcactgttc gaggagatca gcgaagcggc ggacgtgctc
     1081 gtcctcgccg gcgacctgac caatttcggc aagacggcgg aggccgagaa cctcgcggag
     1141 gacctgcgcg gctgtaccat ccccgtcgtc gcggtgctcg gcaatcacga ttacgaggaa
     1201 gggcagccgg acgaggtcgc ccgaatcctc cagtcggcgg gggtcaccat gctcggcgag
     1261 caggcgacgg tgatcgaggg cgtgggcttc gccggcgcca agggcttcat cggcggcttc
     1321 ggccggggcg agctcggcgc cttcggcgag gatgcgatca agctcttcgt cgacgaggcg
     1381 cgcaacgagg cgaggctgct cgagaacgca cttcgctcat tgcggaccga acgggtcgtc
     1441 gcggtccttc attacgcccc gataccggag acggtggaag gcgagccgct cgaaatctat
     1501 ccgttcctcg gctcctcccg gctcgcccac gcgatcgacc gcttcgacaa cgtcaaggcc
     1561 gtggtccacg gccacgccca tcgcggcgcc tattcgggcc acacgccggg cggagtgccg
     1621 gtgttcaacg tcgcccagtt cgtcgtcgag cccgccttcg gccggcccta cgcgcttctg
     1681 gagatctagc cgtcattccg gcgaaagccg gaatccatga acacggcgcg tctcgtcctc
     1741 gaaccacggt gttcatggat cccggatcaa gtcggggatg acggggcctc tcagttcggg
     1801 cgcaccccaa ccgccgccgc cggcggcgcc ctcggctcga gcaaggccgc gctctccagc
     1861 cgatccagcg cccgatgggc ggcgacgtac cgccgggcgt tggcgatcca ggggtcggcc
     1921 cgctcatggc cggggagccg gagcacttcg agcaaggcca cctcgacctg ccccgcctca
     1981 aggctctggc cggctcggcg caggcgctcc gcgggctggg tcgagggcgt gttctcgcgc
     2041 cggatggtga cgaggccggc catctcggtc ttgaaggccg cccaccaggt ctggccctgg
     2101 ccgccgccgg tgaggtgggg cgccacctcc tggaactgct tctgaaggct ctggaccgtg
     2161 accggggagc gcgcgacggt gagaacggtc gccaccgcct gcggctcggt gccggcgaaa
     2221 cgctgcctca gcagggtctc gatatagccc aaagcgactc cgcgatcgat cgcccgccgc
     2281 gccgcgaagg cgacgagcag gccttccgcc cggtcggcat tgcccaccgc ggtgcgcgac
     2341 tggaggtcga gcagggccag cctttcctcg agccggttga cccggcgggc cgtctccgga
     2401 tcgatcacca gccgctcgcc cgcctggggc tcgggcggcg ggccgaccgc gggctctcga
     2461 tccacagtca tcggctgagg cgcgggctcg accgccgggg ccggtgccgg cgccactccg
     2521 aggaaggtcg cggcattgct ccaattggcg agcagccagc ccatcgccgc gagccccgcc
     2581 aggaaggcga cgagcggcag cgccagcaaa gtccagagcg gccgcttcgg cttcgccgtc
     2641 accacccgtc cgggctcata gtccattgcc gtccctcacc acgtccacgc catgattgca
     2701 cagctttacg gcaagctcca gcaaggcttg atcgcgcggc gccgccgcaa ccgccttcgc
     2761 ggcccatccc tcgcccgccg cggcggccgc gcccgggctg atcgcggcga tcgcgatggc
     2821 gctcctgtcg tccgtcagct cggcgaaccg ggtcccggcg cgcgcggaat ggatcatcgc
     2881 caccgccggc ccctcgaacc gcccgtgcgg caccggctcg gccgaataga cgatccgacg
     2941 gtcgatcgcg acgcccgccg cgtccactgg gagatgctcg cgaccgcacg gatgaagcgc
     3001 ttttttcacc cctgccgccg ccatcatcgc cgcggcggcc gctccgtcgc agggcccggc
     3061 tcgaaccttc gcgaacccgg ccgagcgggc cgccgccgcc gtgctttcgc cgaccgcgaa
     3121 gcagggcaaa gcggtgagcc ccgccggggc gtggcgcgcg ccgttggcgc tggtcagaag
     3181 gacagcctcg tagcgggccg catcgacggg cggtccttcc acgcttctga tcgcgaagag
     3241 cggtgcgacg agcggatcga gacccatggc gcgggctcgt gccgcggtct cggatgcgcc
     3301 cggctccggc cgcaggatga agagcttcac agcgcgaaca gcgctcgcag cctggacggc
     3361 gcccggtcca gcatttcgcg ggcaagctcg agcggcgcct tgtcgtcacc gctccggaat
     3421 tgccggtcgc atttctccac ctccatcccg tcctcggtca ggatctcggc gcgcaggcga
     3481 atctggtcgc cttccagggt ggcaagcgcc gccaccggcg aacggcagct cccgcccagc
     3541 ccctccagca gccggcgctc cgcggcgacg caaagatggg tggcgcgatg gtcgatcgcc
     3601 gcgaccaggg tccgcacccg ccgatcggag cttcggacct ccactccgat cgccccttgc
     3661 gcgggagcgg ggaggaaatc gtcgaggagg attccgcaat ccgagcggcc cagccggtcg
     3721 agcccggcgg cggccagaag ggtcgcgtcc acttcgcccc tctggatctt gcggaggcgg
     3781 gtttcgacat tgccccggaa gggcacgatg accaggtccg gccgccgagc cagcaactgg
     3841 gccgcacgcc ggggcgatga ggtccccaca cgagcgccct cagggagcag ggcgaggctc
     3901 ggcgctccca ccagccggtc gcacacgtcc gcccgctcca gcatcgcgac gagggcgagc
     3961 tcgggcggcc ggatcgtctc gacgtccttc atcgaatgga cggcgaggtc gatccgccct
     4021 tcgataagcg ccgagtccag ctccttggtc cacaaggcct tgccgcccat ctcggagatc
     4081 gaccggtcgc ggagctggtc gccgctggtc gcgatcggct cgatctcgat cagctcgggc
     4141 ccccagccat gagcgtcgcg aagcgccgtc gccaccagcc gcgcctggac gagcgcgagc
     4201 ggcgaaccgc gggttccaag gcgaaggacc gactgcatgc gccgcttgtg ctagcgggtg
     4261 cggggatggc agggaagcgg gcatgagcct catcctcggc attgaatcct cctgcgacga
     4321 gacggcggcc gccctggtga cctccgatcg ccacatcctc gcccattgcc tggcggggca
     4381 ggagagcgcc cacagcccct tcggcggagt cgttcccgag atcgcggcgc gagcccatgt
     4441 cgagatcctg ccggggctga tcgagcaggc gctggcggaa agcggagtcg cccttgccga
     4501 agtcgacgcc atcgccgcga ccgccgggcc cggtctggtc ggcggagtca tggtcgggct
     4561 cgtcgccgcc aaggggctgg cgctcgccac cggcaagccg ctcgtcgccg tcaaccatct
     4621 cgagggacat gccttgtcgc cgatgctggg cgatccggcc ttggccttcc cctatctgct
     4681 cctcctcgtc tcgggcggcc attgccagct tctccatgtc gagggtctcg gccgctaccg
     4741 ccggctcgcc accacaatcg acgacgcggc gggcgaggcg ttcgacaaga cggccaagtt
     4801 gctcggcctc ggctttcccg gcggcccggc ggtcgaaagg gcggctctgg ccggcgatcc
     4861 cgccgccgtg ccgctgccga ggcccctgtt gggttcggcc gagccgcatt tttcgttcgc
     4921 gggcctcaag agcgcggtgc ttcgcgcgcg ggacagcggc cttcatgcaa ccggcgacat
     4981 cgccgcctcc ttccagcagg ccgtcgtcga cagcctcgtc gaccgaaccc gccgggcgct
     5041 cgagcgcgtg cccgaggcga cggcgctggt cgtcgccgga ggcgtcgccg ccaacgaaag
     5101 cgtgcgctcg gccctaaccc ggctcgccga cgcgcatggc ctgcccttcg tcgccccgcc
     5161 gctctggctg tgcaccgaca atgcggcgat gatcgcctgg gcgggcgccc ttcgcttcga
     5221 cgcgggcctc accgatccgc tcgacgcccc cgcccgggcc cgatggccgc tcgatccgga
     5281 cgcggaaaag gtccgcggcg ccggggtgaa ggcgtgagcg ggcggccgat cggagtcctt
     5341 ggcggcggcg cctggggcac cgcgctcgcc cagatcatgg cctccgacgg cacgccggtg
     5401 cggctgtggg cgcgggaggc cgacgtggtg gaggcgatca acacccgtcg cgagaacccg
     5461 ctgttcctcc ccggccagaa gctctcggcc gcgatccgcg ccaccagcga tctcacatgg
     5521 gtggcggagg tgagccccgt cctcgtcgtc acgccggcac agcacatgcg ttcggtgctg
     5581 agcgaccttc cggcccatgc ggaaactctg atcctttgct ccaaagggat ggaggccgag
     5641 agcctgcggc tgatgcacga ggttgcccga gaggagcacc cggggtcccc ggtcgccgtg
     5701 ctgtccggac cgaccttcgc gcacgaggtg gcggccgggc tgccgacggc ggtcacgctc
     5761 gcctgcgagg atctcgactt tgcgcgggac cttgccgccc gggtcgcgag gccgtccttg
     5821 cgcccctatg ccgcgagcga catcgtcggc gcggagatag gcggagcggt caagaacgtg
     5881 ctcgccatcg cctgcggcgt ggtcgaaggc cgccggctcg gcctgaatgc tcgctccgct
     5941 ctcatctcgc gcggcttcgc ggagatgacc cgcttcgggc tggcgagggg cgcccgggcc
     6001 gagaccctct cgggcctctc cggcctcggc gacctcgtcc tgacctgctc gtcggtgagc
     6061 tcgcgcaatt tcagcctcgg caagggcctc ggcgaaggcc gcccggcggc ggagcttctc
     6121 gccgaccggc gcacggtggc ggaaggcgcc cataccgctc cggtgctggc caaggcggcc
     6181 cgggaggcgg gagtggacat gcccatcgtt tcggcggtgt gcgcgctcct ggcgggcgag
     6241 gccggagtcg acgaggtggt ggagcgattg ctcgcaaggc cgctgaggtc ggaaggggcc
     6301 taatcgtcat cccggcgaaa gccgggaccc caggacgaga agtctcggcg gggctccccg
     6361 ggatcccggc ctccgccggg acgacgctgt ctccgcagcg tcagaaatcc cagcagatgc
     6421 cctttttctc ccagtcgccg taacgggtcg gctcctcgcc cttgggcccg ccatgctcct
     6481 cgccctcggc ggcgggaccg ggctccggca ccggcgggct cggcgacagg tgggccggcg
     6541 gcttcacatg gccggggcgc cgcccggccc tgccgattga agatggctct ctcatccccc
     6601 acatttcggg acacggaggc ctggacgcaa ggatgaacca ttttcgaacc ctgattctgc
     6661 tcgcggcgat gaccgccttg ttcatgggcc tcggcttcac cctcggcggc ccgcgcggcg
     6721 ccttcctcgc cctgatcgtc gccgcggcga tgaacctgat cacctactgg aacgccgacc
     6781 ggatcgtcct ctccatgcac aatgccaagg aggtgacggt gagggattgc ccgcagctgg
     6841 tcgggatcgt ccatgatctc gcggcccgag cgggcctccc gaacccgcgg gtctatctcg
     6901 tcgactcgcc ccaccccaac gccttcgcca ccggccgcga ccccgcccat gccgccgtcg
     6961 ccgcgaccac cggcctgctc ggcctcctca ccaaggagga agtcgcggcg gtgatggcgc
     7021 acgagctcgg ccacgtccgc aaccgcgaca ccctggtcat gacgatgacg gcgacgatcg
     7081 ccggcgccat ctcgatgctc gccaatttcg gcttcttctt ccgcggcgac ggcgagcgct
     7141 ccaatccgct ggcgatgatc gcggcggtcc tgctcgcccc gttcgcggct atgctcgtcc
     7201 agctcgcgat cagccgcacc cgcgaatatg gcgccgaccg cgccggagcg gagatctgcg
     7261 gcgacccccg agccctcgcc tcggcgctgg ccaagcttca ccagggcgcc gcgcgggtgc
     7321 cgagcccggt cgcgatgcgc aacccggcgg cggcgtcgct ctacatcgtg ccctcgggca
     7381 ccggtcgcga cagcctcttc tcgacccacc ccgataccgg caaccggatc gccgcccttc
     7441 aggcgatggc gagcgagttc gcgccccccc tgcgcgctcc ggcggcgacg gccgtgcccc
     7501 gcacccgccg gagcggagcc agcgcgctca atcccctggg ccgacgctcc cggtgaaccg
     7561 cccgtccgat ccggccggcg tccccgcccg ccgagccgca ttgagcctgc tcgacgcggt
     7621 gctccgcaag ggtctgccgc tcgaggccgc gctcgacacc gccgcccgcg gcctggcgcc
     7681 gaacgaccgc gggctcgccc acgccatcgc cgccgagacc cttcgccggc tcggcgatct
     7741 cgacgccctg atcgacggcg ccacccgcaa ccgcctcccc gacgacgcca aggcccgctt
     7801 cgcccttcgc atcgctctgg tccaggcgct ggcgctggac actccgcccc acgcggcgat
     7861 ctcgaccgtc ctgcctttgg tcgacggcgg gccgcgcaag ctcgtgcacg gcgtcttcgg
     7921 aacgctgatg cggcagcaag cgcggcttcc cgccttgcct tcgcttccgg ccgcggtgga
     7981 ggcgcgctgg acccgcaact ggggcgaggc ggtcgcgatc gaggccgccc ggctcctcgc
     8041 cgcgccgccg ccgctcgacc tcgtcttcgc cgcaggcacc cagccgcccg ccgagatgga
     8101 gggcgtcagc ctgatgccgg gccatttgcg ccttcccgcc cgagcggcgg tggccggcct
     8161 caccggctat ggcgaaggcg gctggtgggt ccaggacatc gccgcctcgc ttccggcgcg
     8221 cctgctcgga tccggcgaag gccgaaccgc cctcgacctg tgcgcggcgc cgggcggcaa
     8281 gacgatgcag cttgccagcg ccgggttcga ggtgaccgcg gtcgattcgt ccgaaagtcg
     8341 tctcgcgcga ctttccgaga atctcgctcg gaccaatctc tccgccaagg tgatcgcggc
     8401 tgacatcctg cgctggtccc cgcccgacaa ggtggacgcc atcctcctcg atgccccctg
     8461 ctcggcgacc ggaatctacc gccgccaccc cgacgtcctc caccgggtcc gcccgcgagc
     8521 gatcgccgag ctcgccgagg ggcagaaggc gatgctcgcc cgggccgccg gctggctgaa
     8581 gccgggcggg accctggtct attcggtctg ctcgctggag ccggaggaag gcgaggcggt
     8641 ggtcgaggcc ttcctcgccg accgccgcga cttcgaggcc gcccatatcg aggccggcac
     8701 gctcccggcg gagatcccgg tcgaaggcaa ccgcgtccgc atcctccccg gtctgttcgc
     8761 cgaagcgggc ggcgccgacg gcttcttcat cgcccgcttc cggcgcgcat agcgtcatcc
     8821 cggcgaaagc cgggacctca ggacaagaag tctcggcggg cctctccgag gtcccggctt
     8881 tcgccgggat gacgggcagc ttactgcccc ggcgactccg gcggctcctg cgacggaccg
     8941 tcgggctcga ccggagccca ataagcattg cagggctcca tgccccagac ataaaatctg
     9001 taccagccgc cccattcctc gatatattca cagcaatcat atggagaatt cacggcatag
     9061 catcggtcgg gtccgccccc gccgccggtc tgggccgacg cgggcgtcgc gaccagcgcg
     9121 gaggctgcca gcaaagttga agcggcaaag gtggcgaata gtctcttcat cccgttcagc
     9181 atcttcactc tcccggtttc gtttttttgc tcgcaccggg gcatctctcg ccgtcgggcg
     9241 cagcttcagg cttccagcga gcccgcccgg ggtgcgaccg gagccgcgcc ctcgaagcgg
     9301 gcggcgggct tattcctccg gcgactcgga cggcttcagc ggctcttccg gacggcggtc
     9361 gtcgcgggcg ccgtcgtacc agatctgctg gcaaggatcg tacagtctgc aacagtacag
     9421 aatcccctgg agggtctggg tgcaataaac gggatcgtcg ggattgccgg tgccggagcc
     9481 cgtctgtgcc gccgcgggcg tcgcgcccag cgaagccgcc atcaccattg cggcgcaggt
     9541 ggccgacatc ctcttaattg cgttcgtcat ccccattctc ccttgtcggc cgagcggcca
     9601 ttcaggattg tttgcgtggc gacgccgccg gctcttgcgc aggatggccg ttcgggctcg
     9661 tcaggagact tcgcccggtc ccggcgagac tggctggggc gcggccggag ccgcgcccgc
     9721 gaaccggccg cgcggcggac ctattgcccc gacgactccg ccgacgcctc ctcgggacgg
     9781 atcaccggat cggaagccgg gctttcgcac ggcacgacca gttcgggata gccgcagcaa
     9841 tctttgaaat aatcatagga tccgtagggc gtgcagccgg cccagggatc gtccggatcc
     9901 tcgacgggcg gcggcggcgg cggcggcttc ttagccgcgc tcgcgggcgc tgcggccagc
     9961 gaggctgcca gcaggatgga ggcggcaaag gtaccgcgca ctctctttct tccgttcgac
    10021 atgttccgtt ccttgttaga ctgccgggtg gtgcggcagg acttgtcgct ccgccgactc
    10081 cgccgggccc tcgcgcaacc ggccggggcg cggccggagc cgcgcccacg gaccggactc
    10141 agccggcggc cgcttccgat ttcgcttcga gaggaatgat gatcgggtcg acgggaggat
    10201 tggggccctg gcacgggatg atcagctccg ggtaatcgca gcattcggcc ccgtagatga
    10261 ccggtcccca gggcgtgcag ccgtaccagg gatcgtcggg atcctcgacc ggcggaggcg
    10321 gcggcttctt cgccatggcg ggcgccgcgg ccagcgaagc cgccatcagg acggaagcgg
    10381 caaaggcccc gctcaatctc tttctcgtgt tcgacatgtc cctctccttg ctcgactggc
    10441 gccaaggcgg atcttgcaga tgccgtccgc cggcgagtcg cttgcgcaag gttaatccca
    10501 agtcccgccg gacaatatag cgaatcatcc ggtttgacga ttatttagaa ccgaaatggc
    10561 tgtaagttcc ggcctccccg aatgactgca cga
//
LOCUS       DASWNU010000579         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_491191, whole genome shotgun sequence.
ACCESSION   DASWNU010000579 DASWNU010000000
VERSION     DASWNU010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491191"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VGB57_11530"
     CDS             complement(<1..336)
                     /locus_tag="VGB57_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0132024.1"
                     /translation="MPIELKMPALSPTMEEGTLAKWLVKEGDTVAAGDLLAEIETDKA
                     TMEFEAVDEGTIAKILVAEGTDEVKVGTVIALIAGEGEEAGAAASAPTAAKEPVAKDG
                     GETGAGQPAT"
     gene            547..1002
                     /locus_tag="VGB57_11535"
     CDS             547..1002
                     /locus_tag="VGB57_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0132025.1"
                     /translation="MRSYLVVIDETSEARAALRYAARRASGTGGGVILLAIVPPAEFV
                     QWGGVQAAMEEESRLRAEAMVLQASGAIVEEAGIEPTIIVRQGEPEKAIADLLAERDD
                     VAALVLGAAAEGGPGPLVSHFSGAVAGTLPCPLVVVPGRLGDEDLDRLS"
     gene            complement(1122..2108)
                     /locus_tag="VGB57_11540"
     CDS             complement(1122..2108)
                     /locus_tag="VGB57_11540"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY0132026.1"
                     /translation="MSKLWLIGLAASAAWTPAAIAQGAPPPPPPPPMAHGAHAPGHGG
                     HGAMVGHSGGGHRAVPPGMHGGGNMKGGGHMRGGGHMKGGGGHMISGGHMKGMKGMRG
                     GGHFNGMKGWQRFGRGHILPPHFRNRQFFVHNWQSFGWPAPMPGGNWVRYHDDALLID
                     GHGRVVDSRYGWDWDGRGEHRAYDDGGYDEGYAEGYGRDDYDDYGRDDDYAGGQHGGG
                     YERDCRGDCGPVRHVPPPPPHPGYGYGSGYGSSYGYGAGCACGPVVVTETITTTAPVM
                     ETHTYYETVTEHRAAPRRHYRKPAVRHYSKPVVRRSVKPAYWAPAPRPGERG"
     gene            complement(2164..>2605)
                     /locus_tag="VGB57_11545"
     CDS             complement(2164..>2605)
                     /locus_tag="VGB57_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0132027.1"
                     /translation="GEPDAVANMANVAALLWETLPDLNWAGFYRNVGGELVLGPFQGR
                     AACIRIPFGRGVCGAAAESLEAQLVEDVEAFPGHIACDSASRSELVVPLVHQGELVGV
                     IDLDSPGLGRFDGEDLAGCLELARLLAPRLGAGSKAVPEALKKS"
BASE COUNT          376 a          981 c          848 g          400 t
ORIGIN      
        1 agtcgcgggc tggccggcgc cggtctcccc gccgtccttc gccaccggct ccttcgccgc
       61 ggtcggggcg gaggcggcgg cgccggcctc ctcgccttcg cccgcgatga gcgcgatgac
      121 cgtcccgacc ttcacctcgt ccgtgccctc ggcgaccagg atcttcgcga tcgtgccctc
      181 gtcgaccgct tcgaactcca tcgtcgcctt gtcggtctcg atctcggcga gcagatcgcc
      241 cgcggcgacc gtgtccccct ccttgaccag ccacttggcc agcgtccctt cttccatggt
      301 cgggctgagg gccggcatct tcaattcgat cggcatggcg cgcggaagct cctgacggca
      361 gtgctcggaa cgccaaacag caacaggagg ggccgggtca agcccgtcct gagcgcttcg
      421 gccccgagcg cttcggccca gagcgcttcg gcccagagcg ctcggccctg accgccgccc
      481 tgtcggggac cgccgcttgc gcccccggac gatctggcgc agcatcgccg cgagacggag
      541 gggtggatgc gctcgtatct ggtggtgatc gacgagacga gcgaggcgcg cgccgccctg
      601 cgctatgccg cgcgccgggc ctcgggcacc ggcggcgggg tcatcctcct cgccatcgtt
      661 ccgcccgccg agttcgtcca gtggggcggg gtgcaggcgg cgatggagga ggaatcgagg
      721 cttagggccg aggcgatggt gctccaggcc tcgggcgcga tcgtcgagga ggccggcatc
      781 gagccgacca tcatcgtccg ccagggcgag cccgaaaagg cgatcgccga cctcctcgcc
      841 gaacgcgacg acgtcgccgc cctcgtcctc ggagccgccg cggaaggcgg acccggccca
      901 ctggtcagcc atttctcggg tgccgtcgcc ggaaccctgc cctgccccct ggtcgtcgtc
      961 cccggccgcc tcggcgacga ggatctggat cggctgagct agcgaaaatc ctccccatgc
     1021 ttcgcatggg gaggggtagc tgatgaggct tctgccaatt acccctccac cacccttcgg
     1081 gtggtccccc tccccgagca agctcgggga ggaatcaagg cttagccccg ctcgcccggc
     1141 ctcggcgcgg gcgcccagta ggcgggcttg accgagcggc ggacgaccgg cttgctgtaa
     1201 tggcgtaccg cgggcttgcg gtaatgccgg cgcggcgcgg cgcggtgctc ggtgaccgtc
     1261 tcgtaataag tgtgggtctc catcaccgga gcggtggtcg tgatcgtctc ggtgaccacg
     1321 accggcccgc aggcgcagcc cgcgccatag ccgtagctcg aaccgtagcc ggaaccgtag
     1381 ccatagccgg gatgcggcgg cggcggggga acatggcgga ccgggccgca gtcgccgcgg
     1441 caatcccttt catagccgcc gccatgctgg ccgcccgcat agtcgtcgtc gcgtccgtaa
     1501 tcgtcgtaat cgtcgcggcc gtagccctcg gcataaccct cgtcatagcc gccgtcgtca
     1561 taggccctgt gctcgccgcg cccgtcccag tcccagccgt agcgcgagtc caccacccgg
     1621 ccatggccgt cgatcagaag ggcgtcgtca tggtagcgga cccagttccc gcccggcatc
     1681 ggcgccggcc agccgaacga ctgccaattg tggacgaaga attgccggtt ccggaaatgc
     1741 ggcgggagga tatggccgcg gccgaatcgc tgccagccct tcatcccgtt gaaatggccg
     1801 cccccacgca tgcccttcat gcctttcata tggccgccgc ttatcatgtg accgccgccg
     1861 cccttcatgt ggccgccgcc cctcatatgg ccgccgcctt tcatgttgcc gccaccgtgc
     1921 atccccggag gcacggcccg atgcccgccg ccgctgtggc cgaccatggc gccatggccg
     1981 ccatgtcccg gcgcgtgcgc gccgtgggcc atggggggcg gaggcggagg cggcggagcg
     2041 ccctgcgcga tcgccgccgg agtccaggcg gccgacgccg ccaagccgat tagccagagc
     2101 ttgctcatat ccgtcccctg tgcgcgagtg cttgcgtgaa gccccggcag gagtcggggc
     2161 ccgttaagac ttttttaacg cttcgggcac ggctttgcta ccggccccca gccgcggagc
     2221 gagcagccgt gccaattcga gacatcccgc caggtcctcg ccgtcgaacc gccccaggcc
     2281 gggactgtcg agatcgatca ccccgaccag ctcgccctga tgcaccagcg gcaccaccag
     2341 ctccgatcgc gaggcggaat cgcacgcgat atgccccgga aacgcctcca catcctcgac
     2401 cagctgagcc tcgagcgact ccgccgccgc cccgcaaacc cccctgccga acggaatccg
     2461 gatgcacgcc gcccgcccct ggaacggccc aagcaccagc tcaccaccga cattgcgata
     2521 gaagcccgcc cagttcagat cgggcagcgt ctcccacagc agagccgcga cattggccat
     2581 gttggcgacc gcgtccggct ccccg
//
LOCUS       DASWNU010000580         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_22454, whole genome shotgun sequence.
ACCESSION   DASWNU010000580 DASWNU010000000
VERSION     DASWNU010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22454"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..894
                     /locus_tag="VGB57_11550"
     CDS             109..894
                     /locus_tag="VGB57_11550"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY0132028.1"
                     /translation="MLLAVDAGNTNIVFALLEDGVIRARWRIATDPRRTADQYAVWLH
                     QLIELEGLPRDVVDAVIVGTVVPRATHNLEVLAAKYFKVEALIAGQAGAEWGIELDVA
                     EPRSVGADRVLNAIAAHALHEGDLIVVDFGTATTFDAVDYSGAYKGGIIAPGINLSLD
                     ALVTAAAKLPRIAIEAPTGNMSVIGRTTEDQMHIGIYWGYVAMMEGLVARMKAEIGRP
                     VKVVATGGLATLFDQHVTLFDEIEPDLTIQGLGLLYKRSQSAT"
     gene            1002..>2027
                     /locus_tag="VGB57_11555"
     CDS             1002..>2027
                     /locus_tag="VGB57_11555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY0132029.1"
                     /translation="MKPGKELLFLALGGSGEIGMNVNLYGCDGKWIMVDLGMTFADPA
                     YPGVELVLPDLTFIEERRDDLLGVVLTHGHEDHIGAIPYLAGDLGVPLWATPFTAGLI
                     AEKLQEEGLERQVKVNIIDIGGTMQIGPFGFRFVPLAHSIPEGNALLIDTPYGKVFHT
                     GDWKLDEFPQLGDPADAAALTRVGDEGILALVCDSTNVFQEEASGSEEQVRQGLDRVI
                     GEAEGRVLVTTFASNAARLSTLGKVAQDTGRRVCVAGRSLDRILRVARSVGYLMDFPE
                     TVDFETAMSLPKRDVMIIGTGGQGESRAALARIAFDQHRIKLSEGDLVVFSSKQIPGN
                     EIAIGRIQ"
BASE COUNT          353 a          691 c          653 g          330 t
ORIGIN      
        1 gatctagcgc cccggcgaag gccgggggcc gctcggctgg ccccggcctt cgccggtgaa
       61 cggccgccgc ctctcgctta tcgtccggcc cccggctaga gagcccccat gctgctcgcc
      121 gtcgacgccg ggaacaccaa tatcgtcttc gcactgctcg aggacggagt catccgcgcc
      181 cgctggcgca tcgcgaccga tcccaggcgg acggcggacc aatatgcggt ctggctccac
      241 cagctgatcg agctcgaggg attgccccgc gacgtcgtcg atgccgtcat cgtcggaacc
      301 gtggtgcccc gtgccaccca caatcttgag gtgctcgccg ccaaatattt caaggttgag
      361 gcgttgatcg ccggtcaggc cggcgcggaa tgggggatcg agctggacgt cgccgaaccc
      421 cggagcgtcg gcgccgaccg ggtgctcaac gcgatcgcgg cccacgccct gcacgaaggc
      481 gacctcatcg tcgtggactt cggcaccgcc accaccttcg acgccgtcga ttattcgggc
      541 gcctacaaag gcgggatcat agcccccggc ataaacctgt cgctcgacgc tctcgtgacg
      601 gcggcggcga agcttccgcg aatcgccatc gaggcgccga cggggaatat gtcggtcatc
      661 ggccgaacca ccgaggacca gatgcatatc ggcatctact ggggctatgt ggcgatgatg
      721 gaagggctcg tggcccggat gaaggccgag atcggccggc cggttaaggt ggtggcgacc
      781 ggcggcctcg ccaccctgtt cgaccagcat gtcacgctct tcgacgagat cgagccggat
      841 ttgaccattc agggcctggg attgttgtac aagcggagcc aaagcgccac ttagccgtcg
      901 ggcggcagtc ccgtcatccc ggcgaaagcc ggggcctcag gacaagaagt cgcgacgatc
      961 cacaatcagg ccccggcttt cgccggggtg acggagatcc aatgaaaccc ggcaaagaac
     1021 tgctcttcct cgcgctcggc ggctcgggcg agatcggcat gaacgtcaat ctctacggct
     1081 gcgacggcaa gtggatcatg gtcgatctcg gaatgacctt cgccgatccc gcctatccgg
     1141 gggtcgagct cgtcctcccg gatctcacct tcatcgagga gcggcgagac gacctgctcg
     1201 gcgtggtcct gacccacggc catgaagacc atatcggtgc gatcccctat ctggccggcg
     1261 atctcggagt cccgctctgg gcgaccccct ttaccgccgg actgatcgcg gagaagctcc
     1321 aggaggaagg gctggagcgg caggtcaagg tcaacatcat cgatatcggc gggacgatgc
     1381 agatcggccc gttcggattc cgcttcgttc cgctcgccca ctcgattccg gaaggcaacg
     1441 ccctcctcat cgacacgccc tacgggaagg tcttccacac cggcgactgg aagctggacg
     1501 aattcccgca gctcggcgat cccgccgacg ccgccgccct gacccgggtc ggcgacgaag
     1561 gcattctcgc cctcgtctgc gattcgacca acgtcttcca ggaggaggcg tcgggatccg
     1621 aggagcaggt ccgccagggc ctcgaccggg tgatcggcga ggccgagggg agggtgctgg
     1681 tcaccacctt cgcctccaac gcggcccggc tgagcacttt gggcaaggtc gcccaggaca
     1741 cgggccgcag ggtctgcgtc gccggccgct cgctcgaccg gatcctgagg gtcgctcgaa
     1801 gcgtcggcta tctgatggat ttcccggaaa ccgtcgattt cgagaccgcc atgtcgcttc
     1861 ccaagcgcga cgtgatgatc atcggcaccg gcggccaggg cgagagccgc gccgcgctcg
     1921 cccggatcgc cttcgaccag caccggatca agctcagcga gggcgacctc gtcgtcttct
     1981 cctcgaagca gataccgggc aacgagatcg cgatcggccg catccag
//
LOCUS       DASWNU010000581         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_515152, whole genome shotgun sequence.
ACCESSION   DASWNU010000581 DASWNU010000000
VERSION     DASWNU010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515152"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1139)
                     /locus_tag="VGB57_11560"
     CDS             complement(27..1139)
                     /locus_tag="VGB57_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0132030.1"
                     /translation="MSDPRRPSEGWGPNENQAPDPSPAGAPAFAGATGVAPSPSPDEA
                     PAYAGVTGVGETLPILPPSPSAGGPSAPSMLQPRILIPFLLITLIWSSTWIVIKDQLG
                     AVPPSWSVTYRFVVAAAAMFAWAAMSGASLRMGRRGHLLAALFGVPQFCLNFNFVYAA
                     EHHVTSGLVATVFALLMVPNSALAWLFLKQKSAPSFIAGSAIACVGVSLLFVQEMRAS
                     PTSAGEVLLGIGLTVLGVLSASAANILQASRGLAAWPLATLLAWGMVYGIVANALYAW
                     VVAGAPVIEARPAYWLGLVYLGLFASALAFPLYFAVMRAVGPGKAAYSSVLVPILAMV
                     FSTMFEGYRWSTLAVAGGLIALAGLVIALRSKAAAA"
     gene            complement(1136..2140)
                     /locus_tag="VGB57_11565"
     CDS             complement(1136..2140)
                     /locus_tag="VGB57_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY0132031.1"
                     /translation="MRFFSDNAAAACPEALAAIASANRLDTAYDGDSWSARLDDSFSE
                     LFETRVTALWVATGTAANALALAELCPPYGSIVCHRDAHIQNDECGAPEFYTNGAKLL
                     LGEGEGAKLTPAAVTALLETVRNDVHQTQPAALSITNATEYGLVYNAGETAALGELAR
                     ARGLGFHVDGARFANAVVSTGASAADLTWRAGVDALSFGFVKNGGISAEALIFFGDRH
                     RQATLYRRKRAGHLLSKGRYLAAQLLAMIEDGVWLRNARVANESAARLAEAAGPERMV
                     LPVEANEIFLKVTPDEARKLREQGFDFYDWGPGEARLVTAWDSDPAHVEALAAAIRAL
                     "
BASE COUNT          420 a          800 c          852 g          332 t
ORIGIN      
        1 cctcccccgg aaagccgggg taggatttaa gccgccgccg ctttcgacct caaagcgatg
       61 accagtcccg caagcgcgat cagcccgccg gcgacggcga gggtcgacca gcgatagcct
      121 tcgaacatcg tcgagaacac catggcgagg atcggcacga gcacgctcga ataagccgcc
      181 ttgcccgggc cgacggcgcg catcacggcg aaatagagcg ggaaggccag agccgaggcg
      241 aacaggccga gatagaccag gcccagccag taggccggcc gggcctcgat caccggcgct
      301 cccgcgacga cccaggcgta gagggcattg gcgacgattc cgtagaccat tccccaggcg
      361 agcagggtgg cgagcggcca tgccgcgagc ccccggctgg cctgaaggat gttggcggcg
      421 gaggcggaaa gcacgccgag cacggtgagt ccgatcccga gcagcacctc cccggcggaa
      481 gtggggctgg cgcgcatctc ctggacgaac agcagggaga ccccgacgca ggcgatcgcc
      541 gagccggcga tgaagcttgg ggccgacttc tgcttgagga acagccaggc gagcgcgctg
      601 ttcggcacca tcagaagcgc gaagacggtc gccaccaggc cgctggtgac gtgatgctcg
      661 gccgcgtaga cgaagttgaa gttcaggcag aattgcggga ccccgaacaa ggcggcgagg
      721 agatggccgc ggcggcccat gcgcaggctc gctccgctca tcgcggccca ggcgaacatc
      781 gccgccgcgg cgacgacgaa gcggtaggtc accgaccagg aaggcggtac cgctccgagc
      841 tggtccttga tgacgatcca ggtcgagctc cagatcaggg tgatgaggag gaaggggatg
      901 aggatccgcg gctgcagcat gctcggggcg gaggggccgc cggcagaggg tgagggaggg
      961 aggatcggca gggtctcgcc cacccccgtc accccggcat aagccggggc ctcgtcgggt
     1021 gaagggctcg gcgctacccc cgtcgccccg gcgaaagctg gggccccggc gggcgaaggg
     1081 tccggcgcct gattctcatt ggggccccag ccttcgctgg gacgacgagg gtcgctcaca
     1141 gcgcccggat cgccgctgcc agcgcctcga catgggccgg gtcgctgtcc cacgccgtca
     1201 ccagccgggc ctcgcccggc ccccagtcgt agaagtcgaa gccctgctca cggagcttcc
     1261 gcgcctcgtc gggcgtcacc ttgaggaaaa tctcgttcgc ttccaccggc agaaccatcc
     1321 gctccgggcc cgccgcctcg gcgaggcggg cggccgattc gttcgccacg cgcgcgttgc
     1381 gaagccacac cccgtcctcg atcatggcga ggagctgcgc ggccagatag cggcccttcg
     1441 acaggaggtg cccggcccgc ttgcggcggt acaaggtggc ctggcgatgc cggtcgccga
     1501 agaagatcag agcctcggcc gaaatcccgc cgttcttgac gaaaccgaag ctcaaggcgt
     1561 ccacgcccgc ccgccaggtc aggtcggccg ccgaggcgcc ggtgctgacc acggcattgg
     1621 cgaagcgggc gccgtcgaca tggaagccga ggccccgggc gcgcgccagc tcgcccaggg
     1681 cggcggtctc cccggcattg tagacgagcc cgtattccgt cgcgttggtg atcgagagcg
     1741 cggccggctg ggtctggtgg acgtcgttgc ggacggtttc gagcaacgcc gtcaccgcgg
     1801 cgggagtcag cttcgcgccc tccccctcgc cgagcagcaa tttggccccg ttcgtataga
     1861 attccggagc cccgcattcg tcgttctgga tatgcgcgtc gcggtggcag acgatgcttc
     1921 cgtaaggcgg gcagagctcg gccaaggcca gcgcgttggc cgccgttccg gtcgcaaccc
     1981 aaagcgccgt cacccttgtc tcgaacagct cggagaacga gtcgtcgagc cgggcgctcc
     2041 aggaatcgcc gtcataggcg gtgtcgagcc ggttggcgga ggcgatggcg gccagcgcct
     2101 cggggcaggc cgccgccgca ttgtctgaga aaaaccgcat ggcggcccct taggcatcgc
     2161 gctcgatact gacaacggtg gcagcgcgaa aatgccgccc gcgtttacta caatttaggt
     2221 gccttgggcc tatccctccc tgtcgagagc caatgaggaa ttgaaggcat gggcgtagag
     2281 cattttgttg acggaccggt ggcgggcaag cgtcaggcca agcgggcgcg cgtcctgctg
     2341 gccgcgaagc tgcagatggc gaacggcggt gagatcgaag cccggcttcg cgatctctcc
     2401 cgca
//
LOCUS       DASWNU010000582         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_74248, whole genome shotgun sequence.
ACCESSION   DASWNU010000582 DASWNU010000000
VERSION     DASWNU010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74248"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VGB57_11570"
     CDS             complement(<1..936)
                     /locus_tag="VGB57_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0132032.1"
                     /translation="MTERQKALVVCPGRGTYGKAELGYLKRFHAKNGDLFTSFDLIRS
                     ERGQPTITELDGADRFSPALHTRGDIASPLIFAASYADFLSIDRSSFDIAAVTGNSMG
                     WYTALAVAGAVGAEQGFRIIDAMGENSQAGEPGGQVLLTLVDEDWRELPGLREQLLAL
                     AATINGRDGCSLYVSIELGGMIVFAGNEPGLAALLAEAPPTPAREPLRLVNHGPFHTP
                     LMTGSSERAMAQLPVDWFGSPETPMVDGRGHIWRPFATGPSALHHYTFVAQILETYDF
                     TRAIQVAVKEHAPDRIVLLGPGDTLGGAIAQALIAI"
     gene            complement(929..3136)
                     /locus_tag="VGB57_11575"
     CDS             complement(929..3136)
                     /locus_tag="VGB57_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0132033.1"
                     /translation="MDAAEAIHQKFVARVRDGNLPRRTAEVRPTEVGLGREAMVELFY
                     SQVASRQMDRLSRKLQARGEGFYTIGSSGHENNAAVADVLRVDDMAFLHYRSNAFQIH
                     RSKKLPGQTACWDMLLSFAASAEDPISGGRHKVIGSKPLAIPPQTSTIASHLPKAVGA
                     AFSIGIARMLKLDETALARDSIVLASFGDASANHSTAQGAFNAAAWAAYQGTPMPLVF
                     LCEDNGIGISTRTPPGWIAASFRNRPGLEYVHCSGLDMADAWRGAREAARIARTRRRP
                     VFLHMDCVRLYGHAGSDVQATYLTKAQIEQDEERDPLLYSAALLIEEELLSGGQILDI
                     YNETEATLARLAEQAILRPKLTTSRDVMASIVPPRRPPARANTPTPEQREALFARDRS
                     MIDKPQHMARLLSWALADLMLAHPNIIVAGEDVGAKGGVYNVTAKLHERFGSCRVINT
                     LLDEQSILGLGIGAAHNDLLAITEIQFLAYVHNAEDQIRGEAATLSFFSSGQYTNAMV
                     IRIAGLGYQKGFGGHFHNDNSLAVFRDIPGLILACPSNGADAVEMLRECVRLALEEQR
                     VIVFVEPIALYMTRDLHEEGDALWTSTYAAPGAAKPIRLGDVGVHGDGTDLAIVSYAN
                     GYYLSRQAERILSEQHGLNSRVIDLRWLAPLNEEGLLDSVAGCERILIVDECRKTGSQ
                     SEALMALFAERAPGAAVRRVTAEDSFIPLGRAATLTLPSRDSIVAAALEMLRD"
     gene            3324..3623
                     /locus_tag="VGB57_11580"
     CDS             3324..3623
                     /locus_tag="VGB57_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY0132034.1"
                     /translation="MTKSEMAAALNEKSAWLPGKRVKLDFGPEGAVMLDGAANQVTEE
                     DGPADTTIKVSWEDWQAMSDGKLDGMTAFMQGKLKVEGDMSNAMQLQGVLAKLRS"
     gene            3801..>3892
                     /locus_tag="VGB57_11585"
     CDS             3801..>3892
                     /locus_tag="VGB57_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HEY0132035.1"
                     /translation="MRKIYPDAAAALDGLLFDGMTICAGGFGLCG"
BASE COUNT          644 a         1201 c         1412 g          635 t
ORIGIN      
        1 gatcgcgatc agcgcctggg cgatggcgcc gcccagcgtg tcgccggggc cgagcaggac
       61 gatccggtcc ggcgcatgct ccttcaccgc gacctgaatc gcccgggtga aatcgtaggt
      121 ttcgagaatc tgggcgacga aggtgtaatg gtggagggcg ctcgggccgg tggcgaaggg
      181 ccgccagatg tggccgcggc cgtccaccat cggagtctcc ggggagccga accaatcgac
      241 cggaagctgc gccatggcgc gctccgacga gccggtcatg agcggcgtgt ggaagggacc
      301 gtggttgacg aggcggagag gctcgcgggc gggagtcggc ggcgcttcgg cgagcaaggc
      361 ggcgaggccg ggctcgttgc cggcgaagac gatcatcccg ccgagctcga tcgagacgta
      421 aaggctgcag ccgtcgcgcc cgttgatcgt ggcggcgaga gccagcagct gctcgcgaag
      481 gcctggaagc tcgcgccaat cctcgtcgac gagggtgagg agcacctgtc cgccgggctc
      541 cccggcctgg ctgttctcgc ccatggcgtc gatgatccgg aagccctgct cggcgccgac
      601 cgctccggcg acggcgaggg ccgtgtacca tcccatcgaa ttgccggtga cggcggcgat
      661 gtcgaagctc gaccggtcga tcgagaggaa atcggcgtag gaggcggcga agatgagcgg
      721 cgaggcgatg tcgccgcgcg tgtgcagggc ggggctgaac cggtcggcgc cgtcgagctc
      781 ggtgatcgtc ggctggccgc gctctgagcg gataaggtcg aaagatgtga aaagatcgcc
      841 attctttgca tggaatcgct tcagatagcc caactccgcc tttccatagg tgcctcgtcc
      901 cggacagacg accagcgcct tctgccgctc agtcacgaag catctccagc gccgcggcga
      961 cgatcgagtc gcgactcggc agggtcaggg tcgcggcccg gccgagcggg atgaacgaat
     1021 cctcggccgt gacccggcgg accgcggccc ctggagcgcg ttcggcgaac agggccatca
     1081 gggcctcgct ctgcgagccg gtcttgcggc attcgtcgac gatcaggatc cgctcgcagc
     1141 ccgcaaccga gtcgagcagg ccttcctcgt tcaggggcgc cagccagcgc aggtcgatca
     1201 ccctcgaatt caggccgtgt tgctcgctca ggatcctttc cgcctgcctg gagagataat
     1261 agccgttggc atagctgacg atggcgaggt cggtgccgtc gccatggact ccgacgtcgc
     1321 cgagccggat cggcttcgcc gcgcccggcg ccgcgtaggt cgaggtccac aaagcatcgc
     1381 cctcctcgtg aaggtcgcgg gtcatgtaga gcgcgatcgg ctcgacgaag acgatcacgc
     1441 gctgctcctc gagcgcgagc cggacgcatt cgcggagcat ctcgacggcg tcggcgccgt
     1501 tgctcgggca ggcgaggatc aggcccggaa tgtcacggaa gacggcgagg ctgttgtcgt
     1561 tgtggaaatg gccgccaaag cccttctgat agcccaggcc ggcgatccgg atcaccatcg
     1621 cgttggtgta ctggccgctg gaaaagaagc tcaaggtcgc agcctcgccg cggatctggt
     1681 cctcggcatt gtggacgtag gcgaggaact ggatctcggt gatggcgaga aggtcgttat
     1741 gggcggcgcc gatgccgagg ccgaggatgc tctgctcgtc gagcagggtg ttgatcaccc
     1801 ggcacgagcc gaagcgttcg tggagcttgg cggtgacgtt gtagaccccg cccttggccc
     1861 cgacgtcctc gccggcgacg atgatgttgg gatgagcgag catcaggtcg gcgagcgccc
     1921 agctcagcag ccgagccatg tgctggggct tgtcgatcat gcttctgtcg cgggcgaaca
     1981 gggcctcgcg ctgctccggg gtcggcgtat tggcccgggc cggggggcgc ctgggcggga
     2041 cgatgctcgc catcacgtcc ctgctcgtcg tcagcttggg ccgcaggatc gcctgctcgg
     2101 cgagtcgggc gagggtggct tcggtctcgt tgtagatgtc gagaatctgg ccgccggaaa
     2161 gcagctcctc ctcgatcagc aaggcggcgc tgtagagaag cgggtcgcgc tcctcgtcct
     2221 gctcgatctg ggccttggtc agataggtgg cctggacgtc cgagccggca tgtccgtaga
     2281 gccggacaca gtccatgtgg aggaagaccg gccggcgccg ggtgcgggcg atccgcgccg
     2341 cctcgcgggc gccgcgccag gcgtcggcca tgtcgaggcc gctgcagtgg acatattcga
     2401 gccccggccg gttgcggaac gaggccgcga tccagcccgg cggggtacgg gtcgatatgc
     2461 cgatgccatt gtcctcgcac aggaagacga gcggcatcgg cgtgccttgg taggctgccc
     2521 atgcggcggc gttgaacgcc ccctgcgcgg tcgagtggtt ggcggaggcg tcgccgaagc
     2581 tggcgaggac gatcgaatcc cgtgccagcg ccgtttcatc gagcttcagc atccgggcga
     2641 tgccgatgct gaacgccgcg cccacggcct tgggcaggtg cgaggcgatg gtgctggtct
     2701 gcggcgggat ggcgagcggc ttcgatccga tcaccttgtg gcggccgccc gaaatcgggt
     2761 cttcggccga ggcggcgaag ctcagcagca tgtcccagca cgcagtctgg ccgggcagct
     2821 tcttcgagcg gtggatctgg aaggcgttgg agcggtagtg gaggaaggcc atgtcgtcga
     2881 ctcgaaggac gtcggcgacc gcggcgttgt tctcgtggcc cgagctcccg atcgtgtaga
     2941 agccctcgcc gcgcgcctgc aatttgcgcg agagccggtc catctgccgg ctggccacct
     3001 ggctgtagaa cagttcgacc atcgcttcgc ggccgaggcc cacttcggtg ggccggacct
     3061 cggcagtgcg gcgcggaaga tttccgtccc gaacccgggc gacgaacttc tgatgaatgg
     3121 cttcggcggc gtccatgcgg gggcgactaa ccgcgttgcc ggtgaatggg aagtaagccg
     3181 tcgtcccggc gaaagcccgg acctcaggac gagaaggcac ggcgggtctc ccggaggtcc
     3241 cgctttcgcc gggatgacgg gagtggcttg acctgacgtt tacgtaaagt agggagaaaa
     3301 ggcacccacc gggaggaaag atcatgacca agagcgaaat ggccgcggcc ctcaacgaga
     3361 agagcgcctg gctccccggc aagcgggtga agctcgattt cggccctgaa ggcgcggtga
     3421 tgctcgacgg cgccgccaat caggtgaccg aggaagacgg acctgccgac accacgatca
     3481 aggtcagctg ggaagactgg caggcgatga gcgacggcaa gctggatgga atgaccgcct
     3541 tcatgcaggg caagctcaag gtcgagggcg acatgtccaa cgccatgcag ctccagggcg
     3601 tgcttgcgaa gcttcggagc tgatttttcc gcgtcgtccc agcgaaggcc gggaccccag
     3661 cgagaaccag cactttagcc tttcacccgc cggggtccca gccttcgccg ggacgacgaa
     3721 cgagttgaaa gcccgttcag gtttccttga cgcgccgcgc ctgccgggct tatcggcgcc
     3781 gcattagacg ggagcggtga atgcgcaaaa tctaccctga tgccgccgcg gcgctcgacg
     3841 ggttgctgtt cgacggaatg acgatctgcg cgggcggctt cggcctgtgc gg
//
LOCUS       DASWNU010000583         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_360235, whole genome shotgun sequence.
ACCESSION   DASWNU010000583 DASWNU010000000
VERSION     DASWNU010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360235"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VGB57_11590"
     CDS             complement(<1..968)
                     /locus_tag="VGB57_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0132036.1"
                     /translation="MLKITRPFLALALGAALIPLAGCAGGSARSRADTRYVARDVNTL
                     YNAAYDNLQRRDYTRAAALFDEVERQHPYSVWARRAQLMSAFSYYALRDYTKSTEAAR
                     RFLSIHPGNRAAPYALYLIALSYYEQISDVTRDQGVTLQAQNALGELIRRYPTSPYSD
                     DARLKLDLVRDHLAGKEMEVGRFYQRRRLWLASAVRFRMVVDQYETTTHAPEALMRLT
                     ETYLALGIPQEAQRAAAVLGANYPNTRWYRRAYELMQQYPTMSPLGRPRVEPATATAT
                     ATDVPASATEVMTTGPEVTAPSEPPTQDPGPPAPQSTPQPSPQPTP"
     gene            1229..1840
                     /locus_tag="VGB57_11595"
     CDS             1229..1840
                     /locus_tag="VGB57_11595"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY0132037.1"
                     /translation="MEYFADAVFAIAFTLSVVELELPREGGASLPHDLAGMWHGFLGV
                     AIASSVIGIHWAHHHFSGAIYRTVGHWFNLATLLFLAAIGFIAFPSRVLADHIDDPEG
                     FPAAATFFMIGLAVIAATWSLKWLVGRLMGNVDDRLDPAYVRRLSRTYFASTVLMIAA
                     AALSVALPVTSLVLGGLILAYFVLPPGTPVYRGAAPAAGGGED"
     gene            1927..3375
                     /locus_tag="VGB57_11600"
     CDS             1927..3375
                     /locus_tag="VGB57_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0132038.1"
                     /translation="MAKRTAFDELWGEGGGDAARPGFEPLAGWLGATPPAELERRQQA
                     AEAAFRQMGITFNVYGDEEAAERIIPFDIVPRIFSAGEWRRVSAGLEQRVRAINAFID
                     DVYGERRILAEGVIPADIVLANPQYCIPVAGARPPQGIYTHICGIDIVRTGPEEFWVL
                     EDNVRTPSGVSYMLENREAMLRLCPELFDLFRVCPVDSYPELLRETLAASAPHGAWEP
                     RCVLLTPGHHNSAFYEHSFLADNMGIELVEGRDLEVDDDVVWMRTTEGRVRVDVIYRR
                     IDDDFLDPLVFNPNSILGVPGLMAAYLAGNVTIANAPGTGIADDKAIYSYMPEIVRFY
                     TGEEARLPNVETWRCREPDGLKYTLDHLPELVVKLVDGSGGYGMLVGPTSTRAEIEAF
                     RAAVMAEPHRYIAQPTLSLSTVPTITAKGLAPRHVDFRPFVLSREDRVVTVPGGLTRV
                     ALREGSLVVNSSQGGGTKDSFILADMPEGGEG"
     gene            3375..>3498
                     /locus_tag="VGB57_11605"
     CDS             3375..>3498
                     /locus_tag="VGB57_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY0132039.1"
                     /translation="MLSRTASNLYWIGRYMERAEFTTSLVEATIRLASLGLRTES"
BASE COUNT          542 a         1207 c         1178 g          571 t
ORIGIN      
        1 tggggcgtgg gttgcggcga gggctggggc gtcgactgcg gcgcgggcgg ccccggatcc
       61 tgggtcggcg gctcgctcgg cgccgtcact tcgggaccgg tggtcatcac ctcggtcgcc
      121 gaagccggca cgtccgtcgc cgtggcggtg gccgtcgccg gctcgacccg cgggcggccg
      181 agcgggctca tcgtcggata ttgctgcatc agctcatagg cgcgccggta ccagcgcgtg
      241 ttcgggtaat tggctccgag cacggccgcg gcgcgctgcg cctcctgcgg aatgccgagc
      301 gcgagatagg tttcggtgag tcgcatcagc gcttcgggcg cgtgggtggt cgtctcatac
      361 tggtcgacga ccatgcggaa ccgcaccgcc gaagcgagcc acagccgccg gcgctggtag
      421 aaccggccga cttccatctc cttgccggcg agatggtcgc ggaccaggtc gagcttcagc
      481 cgggcgtcgt ccgaataagg cgaggtcggg tagcggcgga tgagctcgcc aagcgcgttc
      541 tgcgcctgca aggtcacgcc ctggtcgcgg gtcacgtcgc tgatctgctc gtaatagctc
      601 agcgcgatca ggtagagcgc gtaaggcgcg gcgcggttgc cggggtggat cgagaggaag
      661 cggcgggcgg cctcggtcga cttggtgtag tcgcgcagcg cataataaga gaatgcgctc
      721 atcaactggg cgcggcgcgc ccagaccgaa tagggatgct ggcgctcgac ctcgtcgaac
      781 aaggcggcgg cgcgggtata gtcccgccgc tgcagattgt cgtaggccgc attgtagagc
      841 gtgttgacgt cgcgggcgac atagcgggtg tcggcgcgcg agcgggcgga gccgcctgcg
      901 cagccggcca gcgggatgag cgcggcgccg agcgcgaggg caagaaacgg acgggtgatc
      961 ttgagcatgg ggggcttctt agctgaggct ttgggggagg ccaagggcct gtccccggcc
     1021 aagtcgaagg tcgttcgaat tgaagcgggg caagcgctga accgccggtg aatgacgagt
     1081 cgccggcagg gccgggaacc ccttcccccc tcgccgcttc agcaggtcag ttcaatcgga
     1141 caggtgccct ctgccagacg atcccgctcc agacactccg cgcaccaacg atcaccaaca
     1201 tgacggcgcc attcgcggca cccagcgcat ggagtatttc gccgacgccg tgttcgccat
     1261 cgctttcacc ctgtccgtgg tcgagctcga actgccgagg gagggcgggg ccagccttcc
     1321 ccacgacctg gcgggcatgt ggcacggctt cctgggcgtc gccatcgcct cgtcggtgat
     1381 cggcatccac tgggcgcacc accatttttc cggcgcgatc taccgaacgg tcggacactg
     1441 gttcaacctc gcgaccctgc tgttcctcgc cgcgatcggc ttcatcgcct ttccctcgcg
     1501 ggtgctggcc gaccatatcg acgatccgga gggtttcccc gccgccgcga ccttcttcat
     1561 gatcggcctc gcggtcatcg cggccacatg gagcctgaaa tggctggtcg gccggctgat
     1621 gggcaatgtc gacgaccggc tggacccggc ttacgtccgc cggctcagcc ggacctattt
     1681 cgcaagcacc gtcttgatga tcgccgccgc cgcgctcagc gtcgcgctgc ccgtgaccag
     1741 cctcgtcctg ggcggcctca tcctcgccta tttcgtgctt ccgcccggaa cgccagtcta
     1801 tcgcggagcg gcgccggcag ccgggggcgg cgaggactga agcccacgcc cgccatttga
     1861 gccgccagcc cttttggtgc attgggacca gggcgccgat ggggaagcgg ccgccgacga
     1921 ggagcaatgg cgaagcgaac cgccttcgac gaattgtggg gcgaaggcgg gggcgacgcc
     1981 gcccggccgg gcttcgagcc gctcgccggc tggctcggcg ccacgcctcc cgccgagctc
     2041 gagcgccgcc agcaggccgc cgaagccgcc ttcaggcaga tgggcatcac cttcaacgtc
     2101 tatggcgacg aggaagcggc cgagcggatc atcccgttcg acatcgtgcc gcgcatcttc
     2161 tccgccggcg aatggcgccg ggtctcggcc ggcctcgagc aaagggtgcg ggcgatcaac
     2221 gccttcatcg acgacgtcta tggcgagcgc cgaatcctcg ccgagggcgt gatcccggcc
     2281 gacatcgttc tcgccaaccc gcaatattgc atcccggtcg ccggcgcccg gccgccgcag
     2341 ggcatctaca cccatatttg cggcatcgac atcgtccgca ccggaccgga agaattctgg
     2401 gtgctggagg ataatgtccg caccccgtcc ggcgtctcct acatgctcga gaaccgcgag
     2461 gcgatgctgc ggctgtgccc ggaattgttc gacctgttcc gcgtctgccc ggtcgattcc
     2521 tatcccgaat tgctgcgcga gaccctcgcc gcctcggccc cgcacggcgc ctgggaaccg
     2581 cgctgcgtgc tgctgacgcc cggccaccac aattcggctt tctacgaaca cagcttcctg
     2641 gccgacaata tggggatcga gctggtcgag ggccgcgacc tggaagtcga cgacgacgtg
     2701 gtctggatgc gcaccaccga agggcgggtc cgggtcgacg tcatctaccg gcggatcgac
     2761 gacgacttcc tcgacccgct cgtcttcaat cccaattcga tcctcggcgt cccggggctc
     2821 atggccgcct atctcgccgg caacgtcacc atcgccaacg caccgggcac cggcatcgcc
     2881 gacgacaaag cgatctacag ctacatgccg gagatcgtgc gcttctatac cggcgaggaa
     2941 gcgcgacttc ccaatgtcga gacctggcgc tgccgcgagc ccgacggcct caaatacacg
     3001 ctcgatcatc tgcccgagct ggtggtcaag ctggtcgacg ggtcgggcgg ctacgggatg
     3061 ctggtcggcc ccacctcgac cagggccgag atcgaagcct tccgagcggc ggtcatggcc
     3121 gagccccacc gctacatcgc ccagccgacc ctgtccctct ccaccgtgcc gaccatcacc
     3181 gccaagggcc tcgcgccgcg ccatgtcgac ttccggccgt tcgtgcttag ccgggaggac
     3241 cgggtggtca ccgtgcccgg aggcctcacc cgggtcgccc tccgtgaggg ctcgctggtc
     3301 gtcaactcga gccagggagg cggcaccaag gacagcttca tcctcgccga catgccggaa
     3361 ggaggcgagg gctgatgctg tcgcgcaccg cctccaacct ctactggatc ggccgctaca
     3421 tggagcgggc cgagttcacg accagcctgg tcgaggccac catccgcctg gcctcgctgg
     3481 gcctgcgcac cgagagcg
//
LOCUS       DASWNU010000584         7645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_491328, whole genome shotgun sequence.
ACCESSION   DASWNU010000584 DASWNU010000000
VERSION     DASWNU010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491328"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..362)
                     /locus_tag="VGB57_11610"
     CDS             complement(219..362)
                     /locus_tag="VGB57_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132040.1"
                     /translation="MLPTAAEQRRYRMVIGSVPVIHNDMWDGATAAQKRSMLGWLGRC
                     LRR"
     gene            complement(602..1819)
                     /gene="tyrS"
                     /locus_tag="VGB57_11615"
     CDS             complement(602..1819)
                     /gene="tyrS"
                     /locus_tag="VGB57_11615"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0132041.1"
                     /translation="MSEYRSELLRVLEERGFIHQLTDAGGLDALALKQVIPGYIGFDA
                     TAPSLHVGSLIQIMMLRRLQQAGHKPIVLMGGGTSKIGDPSFKDEARKLLDDETIDSN
                     IASIKRVFERFLTFGDGPTDAILLDNAEWLDKLEYIPFLREIGQHFSVNRMLSFDSVK
                     LRMDREQSLSFLEFNYMILQGYDFLELSRRADCRLQLGGSDQWGNIVNGIELCRRVDS
                     TQVFGLTTPLITTADGGKMGKTEKGAVWLNADSLSPYDYWQFWRNAQDADVGKFLRRF
                     TDLPVDEIARLEALGGAEINEAKKLLADAATALAHGEDAAREAAETARKTFEEGTSGE
                     ALPTLKADGEISLVDALIGLGLCASKNEARRLIVQGGARIDGEAAGDDVTIAVTREVR
                     ISAGRKKHGLLTP"
     gene            1936..2478
                     /locus_tag="VGB57_11620"
     CDS             1936..2478
                     /locus_tag="VGB57_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEY0132042.1"
                     /translation="MARRLSLTPHPDTPSGKVTAVEVELIAGSGDDLLISYFVTGAEH
                     LIVPERRMAARRDGLWETTCFEAFLKPVGGEAYFEFNFSPSTEWAAYRFERHREGRSD
                     LARPVDPFSTRGKEPSDPLLEVDLDLSGLPDVAMRLGLSAVIEEKGGRKSYWALAHPP
                     GDPDFHNASCFTLELPAARI"
     gene            2582..3790
                     /locus_tag="VGB57_11625"
     CDS             2582..3790
                     /locus_tag="VGB57_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY0132043.1"
                     /translation="MVRFGIDRLLADPALRKPLEGKRIALLAHPASVTEDLTHSLDAL
                     IAAGLNVTAAFGPQHGLRGDKQDNMVESDDFTDPAHNIPVFSLYGEVRRPTGQSMGTF
                     DILLVDLQDVGCRIYTFITTLLYVLEAAAEHGKQVWVLDRPNPAGRPVEGLTLRPGWE
                     SFVGAGPMPMRHGLTLGELGRWFARTMKIDVDYRIVEMGGYEPDSSPGFGWPPERIWI
                     NPSPNAANLWMARAYAGTVMLEGTTLSEGRGTTRPLELFGAPDIDAGAVVAEMRRLAP
                     EWLEGCALRQIAFEPTFHKHAGKTCHGVHIHPEGRFYDHDTFLPWRLQSLAFKAIRRI
                     DPGYDLWRDFPYEYEFGKLAIDVINGGPGLREWVDDEAASPADLDALALPDEEAWLEE
                     RRPFLLYPAR"
     gene            3822..4349
                     /gene="def"
                     /locus_tag="VGB57_11630"
     CDS             3822..4349
                     /gene="def"
                     /locus_tag="VGB57_11630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0132044.1"
                     /translation="MAIRPIIEAPDPLLRQVSSPVEAITPEIQTLIDDMFETMYAAPG
                     IGLAAIQVGVPKRILVIDLQDPAEEGGEPVPNPMVFINPEILRTSETPRPYNEGCLSV
                     PDQYAEVERPDAIRARWLDRDGKVHEEELDGMLATCLQHEMDHLEGVLFIDYLSRLKR
                     DMILRKLAKQRKLAA"
     gene            4558..5946
                     /locus_tag="VGB57_11635"
     CDS             4558..5946
                     /locus_tag="VGB57_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY0132045.1"
                     /translation="MDSSLILFVILALAAGAALGWFLGVRQAALYRKERDDRTEDFRK
                     AIADLAGMQGKLDEARQACSDAKVECARVTAGQEERERAFEARLEEVKNAREALSAQF
                     SEIGGKLLGEAQKSFLERAEARFKQSEESAGLGLKALLQPVTDRLQRYEEGVARVEAE
                     RRDAFGNLVGLIESMRVGQEKVSSEAAKLVNSLRNAPKSRGRWGEQQLKNVLETCGLT
                     ENCDFQTEVSVEGEDGRLRPDVIIRIPGGRTLIIDAKVSLNAYQDAFGAVDDAERQIG
                     LAGHAAAMRAHVNALGNKAYQTQFGNSPDYVIMFVPGEHFLSAALEHDPTLWDFAFDK
                     KVLLATPTNLIAIARSVASVWQQEALARNARDIALLGKELYDRLAKVGEDLRRVGTGL
                     NSAVSNFNIFTNSFNSRLVSTGRKFRELDIETGARELEDVPRVEAFASVGEMPRPALI
                     EGSAEGNAEAAE"
     gene            complement(5947..6390)
                     /locus_tag="VGB57_11640"
     CDS             complement(5947..6390)
                     /locus_tag="VGB57_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132046.1"
                     /translation="MFFQLNDRSDGTLCPFRSRNVMTRALLPIALLAAGCAAEQPPAT
                     ASDPSREHYEALGQEPGWRILIHGGRIDYTGSYGEKKISVARPDPRPSFNGRRYVTAR
                     LTVDVTYSRCNDVMSGFGFEHQVTVTADGETYKGCGGARKAEWDM"
     gene            6528..6782
                     /locus_tag="VGB57_11645"
     CDS             6528..6782
                     /locus_tag="VGB57_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132047.1"
                     /translation="MKRKILYTALVAAVMATANPAYAQTQDIYYYYYYENGQLVGEGR
                     DRCWKFGVVPHGEMEWGYATNQFDAVVVGLCQVPWEPIEL"
     gene            complement(6777..7607)
                     /locus_tag="VGB57_11650"
     CDS             complement(6777..7607)
                     /locus_tag="VGB57_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0132048.1"
                     /translation="MPRQITAFSNPLVKDVRALREKKGRRRQGMFLAEGLRILTEARE
                     AGRLPAILFFAAGTSHPLLDTLIAEIEAAHGDVIETNADILHKLSGKDNPQAVVAVYR
                     SLDTSLSRIDRSAAPLWIVAQALRDPGNLGTILRTGDAVGAGGLILVDDCVDPFSVES
                     VRASMGALFTQKIAAAPWPEFLAWLRSGEGELIGTSLRAELDYQAPSYSKPAFLLVGN
                     EAQGLPEAYEAECDLLVKMPMHGKADSLNAAVATAVMAYEVVNQWRALQPVPPPDRPL
                     "
BASE COUNT         1299 a         2508 c         2510 g         1328 t
ORIGIN      
        1 cgaaccatgt tttccctctt tgttctattt tgtaggatac gcacgccttg gggtgtgaga
       61 tattgagggc tgggcgagtc gcggactgcc cgaggctctt tgacattgtg attcgaaaag
      121 cgatcgtccg cggctgcgcc gttgcggggg gcggggcggc gagcactccg gcgggcggcg
      181 gccgaccgga ttgcctcgcc gcgtcggcaa cgacggggtc agcggcgaag acagcgcccc
      241 agccagccga gcatcgaccg cttctgcgcc gccgtcgctc cgtcccacat gtcgttgtgg
      301 atcaccggaa cggagccgat caccatccgg tagcgtcgct gctcggccgc ggtcgggagc
      361 actccgggat ccgcgggttg gtcgctcgag cggaccgagc agacccgcgg gcgggcggtc
      421 cggggcatcc tcatccggcg gttgtcgagg gtgaagacgg cccgggtaac gaattgcagc
      481 ggccatcttc cctcccgtca tcctgaacct gtttcgggat ccagttctcc accggcacga
      541 ccctcatcca gctggacccc gaaacaagtt cagggtgacg atcagcggtt gaggcacgac
      601 gtcagggggt gagcagtccg tgcttcttcc tgccggcgga tatccgcacc tcgcgcgtca
      661 ccgcgatcgt cacgtcgtcg cccgccgctt cgccgtcgat ccgggcgccg ccctggacga
      721 tcaggcgccg ggcctcgttc ttcgaggcgc aaagaccgag gccgatcagg gcgtcgacca
      781 gcgagatctc gccgtccgcc ttcagcgtcg gaagggcctc gccggacgta ccctcctcga
      841 acgtcttgcg agccgtctcc gccgcctccc gcgccgcatc ctcgccatgc gccagcgccg
      901 tcgccgcgtc cgccaggagc ttcttcgcct cgttgatctc ggcgccgccc agcgcctcca
      961 gccgggcgat ctcgtcgacc ggaaggtcgg tgaaccggcg aaggaacttg ccgacgtcgg
     1021 cgtcctgcgc gttcctccag aattgccagt aatcgtaggg gctcaggctg tcggcgttga
     1081 gccagaccgc gcctttctcg gtcttgccca tcttgccgcc gtcggcggtg gtgatgagcg
     1141 gggtcgtgag gccgaagacc tgggtcgagt cgacgcggcg gcacagctcg atcccgttga
     1201 cgatattgcc ccactggtcg gagccgccga gctggagccg gcagtcggcg cggcgggaca
     1261 gctcgaggaa gtcgtagccc tgcaggatca tgtagttgaa ctcgaggaag gagagcgact
     1321 gctcgcggtc catcctcaat ttgacgctgt cgaagctcag catccggttg accgagaaat
     1381 gctggccgat ctcgcgaagg aaggggatat attcgagctt gtcgagccac tcggcattgt
     1441 cgagcaggat ggcgtccgtg gggccgtcgc cgaaggtcag gaagcgttcg aagacgcgct
     1501 tgatcgaggc gatgttggag tcgatcgtct cgtcgtcgag aagctttcgc gcctcgtcct
     1561 tgaagctcgg atcgccgatc ttcgaggttc cgccgcccat cagcacgatc ggcttgtgcc
     1621 cggcctgctg gagccggcgg agcatcatga tctggatgag ggatccgacg tggagcgagg
     1681 gcgccgtggc gtcgaagcct atgtagccgg ggatcacctg cttgagcgcc agggcgtcga
     1741 gccccccggc gtcggtcagc tggtggatga agccgcgctc ttcgaggaca cggaggagtt
     1801 cggatctgta ttcggacatg ggccgtccct agcctccccg tcatccccgc gaaagcgggg
     1861 atcccgcttc ctttcacccg agccgttgga agaagcggga ttcccgcttt cgcgggaatg
     1921 acgtagggcg cggcaatggc ccggcgctta tccctgaccc cgcatcccga cacgccgagc
     1981 ggcaaagtca cggcggtcga ggtcgagctg atcgccggtt cgggtgacga tctcctgatc
     2041 agctacttcg tcaccggtgc cgagcatctg atcgtgccgg aacggaggat ggcggcgcgg
     2101 cgcgacgggc tttgggagac gacctgtttc gaagcctttc tcaaaccggt cggcggggag
     2161 gcctatttcg agttcaactt ctcgccttcc acggaatggg ccgcctaccg cttcgaacgg
     2221 catcgggaag ggagaagcga cctggctcgg ccggtcgacc ccttctcgac gcgcgggaag
     2281 gagccttcgg atcctctcct ggaagtcgat ctcgacctgt cgggcttgcc ggacgtggcc
     2341 atgcgcctcg gcctgagcgc cgtgatcgag gagaaaggcg gccgcaagtc ctactgggcg
     2401 cttgcccatc cgccgggaga ccccgacttc cacaacgcgt cttgcttcac gctcgaactc
     2461 ccggcagcgc gcatttgact tcgtcattgc gaggagcgaa gcgacgcggc aatccagctt
     2521 cggtgttcgt cccgggctca gcgctggatt gcttcgcttc gctcgcaatg acggaattct
     2581 gatggttcgc ttcggtatcg accgcctcct cgccgacccc gccctgcgca agccgctcga
     2641 aggcaagcga atcgccctgc tcgcccatcc ggcctcggtg acggaggacc tcacccactc
     2701 cctcgatgcg ctgatcgccg ccggcctcaa cgtcaccgcg gccttcggac cgcagcacgg
     2761 cctgcgcggc gacaagcagg acaatatggt ggaatcggac gatttcaccg atcccgccca
     2821 caatatcccg gtcttcagcc tctacggcga ggtccgccgc ccgaccggcc agtcgatggg
     2881 gacgttcgac atcctgctgg tcgatctcca ggacgtcggc tgccggatct acacgttcat
     2941 caccaccttg ctctacgtgc tcgaggcggc ggcggagcac ggcaagcagg tctgggtgct
     3001 cgaccggccc aatcccgccg gccggccggt cgaggggctg acgctcagac ccggctggga
     3061 aagcttcgtc ggcgccggtc cgatgccgat gcgccacggc ctgacgctcg gcgagctcgg
     3121 ccgctggttc gcccggacga tgaagatcga cgtcgactac cggatcgtcg agatgggggg
     3181 ctatgaaccc gattcctcgc ccggcttcgg ctggccgccc gagcggatct ggatcaatcc
     3241 aagccccaac gccgccaacc tctggatggc gcgcgcttat gccggcacgg tgatgctcga
     3301 aggcacgacc ctgtcggagg ggaggggcac gacccgcccg ctcgagctgt tcggagcgcc
     3361 cgacatcgac gccggcgccg tcgtcgccga gatgcgccgg ctcgcgcccg aatggctgga
     3421 gggctgcgcg cttcgccaga tcgcgttcga gccgaccttc cacaaacatg ccggcaagac
     3481 ctgccacgga gtccacatcc acccggaagg ccgtttttac gaccacgaca ccttcctccc
     3541 ctggcgcctc cagtcgctcg ccttcaaggc gatccgccgg atcgatccgg gctacgattt
     3601 gtggcgcgac ttcccctacg aatatgagtt cggcaagctc gccatcgacg tcatcaacgg
     3661 cggcccgggg ctgagggagt gggtggatga cgaagcggcg tcgcccgccg acctcgacgc
     3721 tctcgcattg cccgacgaag aggcctggct ggaggagcgc cggcccttcc tcctctaccc
     3781 ggcgcgttga cgaaaagacc tgcaatacct agataatagc catggccatc cgccccatca
     3841 tcgaagctcc ggaccccttg ctgcgccagg tctcctcgcc ggtcgaggcg atcacgcccg
     3901 agatccagac cctgatcgac gacatgttcg agacgatgta cgccgccccg gggatcggcc
     3961 tcgccgcgat ccaggtcggc gttcccaagc gaatcctcgt catcgatctt caggacccgg
     4021 cggaggaagg cggcgagccc gtccccaatc cgatggtgtt catcaacccg gagatcctgc
     4081 gcacctccga gactccgcgg ccctacaatg aaggctgcct gtccgtcccc gatcaatatg
     4141 ccgaggtcga acgccccgac gcgatccgcg cccgctggct cgaccgcgac ggcaaggtgc
     4201 acgaggagga gctggacgga atgctcgcca cctgcctcca gcacgagatg gaccatctcg
     4261 agggcgtgct cttcatcgac tatctctccc gcctcaagcg cgacatgatc ctgaggaagc
     4321 tggcgaagca gcggaagctg gcggcctgat cgtcatcccg gcgaaggccg ggacccatga
     4381 acaccgagtt gcgagggccg gcgccgcggc cgatttcgcg cgccgcgatc atggatcccg
     4441 gccttcgccg ggatgacgtc cttccctcgc cagccagttt tccacaactt aggtgtccca
     4501 cccctttacc atttccgaca cctgccgtag gttcgccctt cgttccaggg gcgcaacatg
     4561 gactcttcgc tcatcctctt cgtcatcctg gccctcgcgg cgggtgcggc gctcggctgg
     4621 tttctcggcg tccgccaggc cgcgctctac cgcaaggagc gcgacgaccg gaccgaggat
     4681 ttccgcaagg cgatcgccga cctcgccgga atgcagggga agctcgacga ggcgcggcag
     4741 gcttgctcgg acgcaaaggt ggagtgcgct cgggtcacgg cgggtcagga agaacgcgag
     4801 cgcgccttcg aagcgaggct cgaagaggta aagaacgcgc gcgaagcttt gtcggcccag
     4861 ttcagcgaga taggcggcaa gcttctcggc gaggcccaga agagtttcct cgaacgggcg
     4921 gaggctcgct tcaagcagtc ggaagagagt gccggcctcg ggctgaaggc gctcttacag
     4981 ccggttaccg atcggcttca gcgctacgag gagggggtgg ccagggtcga agcggagcgc
     5041 cgcgacgctt tcggcaacct ggtgggcctg atcgagtcaa tgcgcgtcgg ccaggagaag
     5101 gtcagcagcg aagccgcgaa gctggtcaac tcccttcgaa acgccccgaa atcgcgcggg
     5161 cgctggggtg agcagcagct gaagaacgtg ctggaaacct gcggactgac ggaaaattgc
     5221 gacttccaga ccgaagtcag cgtcgaaggc gaggacggcc gccttcggcc cgatgtcatt
     5281 atccgaattc ccggcgggcg aactctcatc atcgatgcaa aggtgtcgct caacgcctat
     5341 caggacgcct tcggggcggt cgacgacgcg gagcgccaga tcggcctcgc cgggcatgcg
     5401 gcggcgatgc gggcccacgt caacgcgctc ggcaacaagg cctatcagac ccagttcggc
     5461 aacagccccg actatgtgat catgttcgtg ccgggcgagc atttcctctc ggccgcgctg
     5521 gagcatgatc cgacattgtg ggacttcgcc ttcgacaaga aggtcctcct cgcgacgccc
     5581 accaacctga ttgccatcgc ccgctcggtc gcttccgtct ggcagcagga ggcactggcc
     5641 aggaacgcgc gcgacatcgc cttactcggc aaggagcttt acgaccggct cgccaaggtc
     5701 ggggaggatt tgcgccgggt cggaacgggg ctcaacagcg ccgtcagcaa tttcaatatc
     5761 ttcaccaact cgttcaacag ccgactcgtc tcgaccggac gcaagttccg ggagctcgat
     5821 atcgagaccg gcgcgcgtga actggaggat gttccgcggg tggaggcctt tgccagcgtg
     5881 ggcgaaatgc cgcgtcccgc cctgatagag ggaagtgcgg agggcaatgc cgaggccgcc
     5941 gaatagtcac atgtcccact cggctttccg cgccccgccg cagcctttgt aggtttcacc
     6001 gtcggcggtc accgtcacct ggtgctcgaa gccgaagccg ctcatcacgt cgttgcagcg
     6061 cgagtaggtg acgtcgacgg tgagcctcgc ggtgacataa cgccggccgt tgaagctcgg
     6121 ccgcgggtcg gggcgggcga cgctgatctt cttctccccg taacttccgg tatagtcgat
     6181 ccggccgcca tggatgagga ttcgccaccc gggctcctgg ccgagcgctt cgtaatgctc
     6241 cctggaagga tcggacgctg tggccggggg ctgctcggcg gcgcagccgg ccgcgagcag
     6301 ggcgatgggg aggagggcgc gggtcatgac gttacgactc cggaacgggc agagggttcc
     6361 gtcggaccgg tcattgagct ggaagaacat ccactccaat acccaaagtt gattgcagac
     6421 gagttcacag tctttacgat cggtccgcga gtcgactcat cggcctcgga cattctgggc
     6481 ccgtcgcccc gccggcatat ctggtgcgtc agcggtggag aaccatgatg aagcgcaaaa
     6541 tcctgtatac cgcccttgtt gctgccgtca tggcaaccgc caatccagcc tacgcgcaaa
     6601 cccaagacat atattactat tattattacg agaacgggca acttgtcggg gaagggcgcg
     6661 atcgatgctg gaaattcggg gtggtccctc atggcgagat ggagtggggc tacgccacaa
     6721 accaattcga tgctgtcgtc gtgggccttt gccaggtgcc ctgggagccg attgaattat
     6781 aatggccggt caggcggcgg aacaggttgc agggcccgcc actggttgac cacctcgtag
     6841 gccatcaccg ccgtcgccac cgccgcgttg aggctgtcgg ccttgccgtg catcggcatc
     6901 ttcaccagca ggtcgcattc cgcctcgtaa gcctcgggga ggccttgcgc ttcgttgccg
     6961 acgagaagga aggccggctt cgaatagctc ggcgcctgat agtcgagctc ggcgcgaagg
     7021 ctggttccga tcagctcgcc ctcgccggag cgcagccagg cgaggaattc gggccagggc
     7081 gcggccgcga tcttctgggt gaacagggcc cccatcgagg cgcggacgga ttcgaccgag
     7141 aagggatcga cgcagtcgtc gaccaggatc aggccgccgg cgccgaccgc gtcgccggtt
     7201 cggaggatgg ttccgagatt gccgggatcg cggagcgcct gggcgacgat ccacaaaggc
     7261 gcggcgcttc ggtctatgcg cgagaggctc gtgtccaggc ttcggtagac ggcgacgacc
     7321 gcctgcggat tatccttacc cgaaagcttg tggaggatgt cggcattggt ctcgatcacg
     7381 tcgccatgcg cggcctcgat ctcggcgatg agcgtgtcga gcagtggatg cgaggttccg
     7441 gccgcgaaga acaggatggc cggcagccgg cccgcctccc gcgcctcggt caggatccgg
     7501 agcccctcgg ccaggaacat gccctggcgg cggcgtccct tcttctcgcg aagcgcgcgg
     7561 acgtccttga ccagcggatt ggagaaagcg gtgatctggc gcggcatcag ttccccggcg
     7621 aaggccgggg cccagacggc gccgg
//
LOCUS       DASWNU010000585         5572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_438902, whole genome shotgun sequence.
ACCESSION   DASWNU010000585 DASWNU010000000
VERSION     DASWNU010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438902"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /gene="der"
                     /locus_tag="VGB57_11655"
     CDS             <1..1193
                     /gene="der"
                     /locus_tag="VGB57_11655"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0132049.1"
                     /translation="IDTAGYEDEDPESLPGRMRAQTIAAVRESDAALFLIDARAGLTP
                     LDEEIGRWLRSETTPVILAANKAEGRAGESGIMEAWSLGFGEPIAMSAEHGEGVVDLF
                     EHLRPHIEREEEEDEDRLGEGPLKLAVVGRPNAGKSTLINRIMEEERLITGPEAGITR
                     DSIAIDWMWRSPGGEERPVRLIDTAGMRKKAKVQDKLEKLAVADGLRAVDFAEVVVLL
                     LDGTLGLEAQDLKIADRVLQEGRALIIAINKWDVAHDQSSLFNGIKAALGEGLAQAPN
                     GPLLTVSAKTGKGLDALLGAAFEVRETWSKRVSTGQLNRWFEDAVEKNPPPAPSGRRI
                     KLRYITQVGSRPPSFVVFGSRLEALPESYRRYLMNGMRRELGFGAVPIRLALRGGKNP
                     YDKG"
     gene            1329..1733
                     /locus_tag="VGB57_11660"
     CDS             1329..1733
                     /locus_tag="VGB57_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY0132050.1"
                     /translation="MDEWTGNQEEVVDWTVFCAARSELGAGFARILSYFREDGEKAVS
                     RIESAMHARDTAGLVLPAHTMKSEARQFGAEPLGALAEEIEFGARRAIEMGFFPDNLL
                     PSVARLRPLYLETITQLEAEANPLVHRRRAGS"
     gene            complement(1786..3069)
                     /locus_tag="VGB57_11665"
     CDS             complement(1786..3069)
                     /locus_tag="VGB57_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEY0132051.1"
                     /translation="MTAATEPPPRIAALDIVRGVAVMGILAMNIVAFAMPFQAYMNPL
                     AYGWESSADFVSWVVSFIFVDGKMRGLFSFLFGASMLLVIERAEAGGQDSASIHFRRM
                     FWLLVFGLIHLYLIWFGDILVGYALVGMIAWFFRNKEPAVLLRWGIALVTVQLMIFAG
                     LAAGAFHLQDAASAPGAAAEILTEWRDLDTKFGVIAGPALAGKMALFQGDYGPLVAYR
                     LGAHAAAPIVGLLFFGWETLAYFLFGMVALRNGFLRGGWSDLAYRRTALAGFGIGIPA
                     YALLAWLLARDGFSVPMVIGIVMAATVPFRPLMVVAIAALIILATRRGGLLTERIAAA
                     GRAAFTNYLGTSLIMTTLFYGYGGGLFGQLSRAQLWLPVVAMWVLMLLWSKPWLDRFQ
                     YGPLEWLWRSLARGSVQPMRRPLSVAPAEAGAPAS"
     gene            3574..4410
                     /locus_tag="VGB57_11670"
     CDS             3574..4410
                     /locus_tag="VGB57_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0132052.1"
                     /translation="MAVRFMGTSTILLDDGTTQILIDGFFSRPGLLLLPIAPNKPVIE
                     SAMARAGIGNRLKAVLVAHSHYDHALDSATIARLTGARLVGSASTANVGRGGGLPNSQ
                     IQVVQHGQELDYGDFHVRVFRSRHAPPMAFPGEIVTPLAPPKWVSSYREGGSYSFLVT
                     HHGFSILIHPSADAPKDLYKNIRADVIFQGVGGLSKQSNDFILRYLQELRWPGAQVVI
                     PVHWDDFTKPLSSGLRLPPKSTDDVAAMMKGLRFYAQIRNFRLRAMPLFDPVDIEAAL
                     AP"
     gene            complement(4429..4908)
                     /gene="bfr"
                     /locus_tag="VGB57_11675"
     CDS             complement(4429..4908)
                     /gene="bfr"
                     /locus_tag="VGB57_11675"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY0132053.1"
                     /translation="MKGDIRVIELLNEALRNELTATNQYWLHYRMLDNWGVKRLAAFE
                     RGESIDEMKHADRLAERILFLDGLPNFQLLGRLRIGETVEEVLRSDLELEIEAVEQLK
                     GAIQHCEAVRDYVSRDLFAEILENEESHVDSLETQFEMIARMGLQNYIQLQSRATED"
     gene            complement(5094..5264)
                     /locus_tag="VGB57_11680"
     CDS             complement(5094..5264)
                     /locus_tag="VGB57_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0132054.1"
                     /translation="MIVCVCNAIRESEVRQAALEGASCPSSAYSRFGRKPRCGQCFSF
                     ARQVIAAAQAAR"
     gene            complement(5313..>5572)
                     /locus_tag="VGB57_11685"
     CDS             complement(5313..>5572)
                     /locus_tag="VGB57_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132055.1"
                     /translation="MPIDAAHIFVTQYADMVTKLATLRPLLAPGGFIWVSWPKKASKV
                     PTDILEDRIRDAILPVTDLVDVKVCAVDSIWSGLKLMARRR"
BASE COUNT          996 a         1812 c         1847 g          917 t
ORIGIN      
        1 tcatcgacac cgccggctac gaggacgagg atcccgagag cctgcccggc cggatgcggg
       61 cccagaccat cgccgcggtc cgcgagtccg acgccgcctt gttcctgatc gacgctcgcg
      121 ccggcctgac gccgctcgac gaggagatcg gccgctggct gaggagcgag acgacccccg
      181 tcatcctcgc cgccaacaag gccgagggcc gggccggcga atcgggaatc atggaggcct
      241 ggtccttggg cttcggcgag ccgatcgcga tgagcgcgga gcatggcgag ggcgtcgtcg
      301 acctgttcga gcatctgagg ccccatatcg agcgcgagga ggaggaagac gaggacaggc
      361 ttggggaagg gccgctcaag ctcgcggtcg tcggccgtcc caatgccggc aagtccaccc
      421 tgatcaaccg gatcatggag gaagagcggc tgatcaccgg tccggaggcc gggatcaccc
      481 gcgattcgat cgcgatcgac tggatgtggc gctcgcccgg cggcgaggag cggccggtgc
      541 ggctgatcga caccgcgggc atgcgcaaga aggccaaggt ccaggacaag ctcgagaagc
      601 tcgcggtcgc ggacggcctt cgcgccgtcg acttcgccga ggtggtcgtt ctcctgctcg
      661 acgggacgct cgggctggag gcgcaggacc tgaaaatcgc cgaccgggtc ctccaggagg
      721 gccgtgccct gatcatcgcg atcaacaaat gggacgtggc gcacgatcag agcagcctct
      781 tcaacgggat caaggcggcc ctgggcgaag ggctggccca ggcgccgaac gggcccttgc
      841 tcaccgtctc ggccaagacc ggcaagggcc tcgacgcttt gctcggcgcc gccttcgagg
      901 tgcgcgagac ctggtcgaag cgggtctcga ccggccagct caaccgctgg ttcgaggacg
      961 cggtggagaa gaaccctccg cccgcgccga gcggccgccg gatcaagctt cgctacatca
     1021 cccaggtcgg atcgcggccg ccgagcttcg tcgtcttcgg aagccggctg gaggcgcttc
     1081 ccgaaagcta ccgccgctac ctgatgaacg ggatgcgccg cgagctcggc ttcggggcgg
     1141 tcccgatccg cctcgccctt cgcggcggca agaatccgta cgacaaaggg tagccccgtc
     1201 attgcgagga gcgaagcgac gcggcaatcc agttttgcgt catgggccgg actggattgc
     1261 ttcgcttcgc tcgcaatgac ggcctcgcgt caaccggtcc tttactccca cgggcctagt
     1321 ctcgcttcgt ggacgaatgg accggtaatc aagaagaggt cgtcgactgg acggtcttct
     1381 gtgcggcgcg cagcgagctg ggcgcgggat tcgcgcgcat cctcagctat ttccgggagg
     1441 atggcgagaa agcggtgagc cggatcgaat cggccatgca cgcccgcgac acggccggcc
     1501 tcgtccttcc ggcccacacg atgaagtccg aggcccggca attcggcgcc gagcccctgg
     1561 gagcgctcgc cgaggagatc gagttcggcg cccgccgggc aatcgagatg ggcttctttc
     1621 ccgacaatct gcttccgtcc gtcgcccggc tccggccgct ctatctcgag accatcaccc
     1681 agctcgaggc ggaggccaac ccgctggtcc accgccgccg cgctgggagt tgaccgtcat
     1741 cccggcgaaa gccgggatcc cggcgggtga agagcaccgg cgaccttagc tcgctggggc
     1801 cccagcctcc gctggggcga cggagagggg cctccgcatc ggctggaccg acccgcgcgc
     1861 cagcgagcgc cacagccatt cgagaggccc atattggaag cggtccagcc agggcttcga
     1921 ccagagcagc atcagcaccc acatcgccac caccgggagc cacaattggg cccggctgag
     1981 ctggccgaac aggccgccgc catagccgta gaacagggtg gtcatgatca ggctggtgcc
     2041 gagataattg gtgaaggcgg cgcggccggc ggcggcgatc cgctccgtca gaagcccgcc
     2101 gcggcgggtc gcgaggatga tcagggccgc gatcgccacc accatcagcg gccggaacgg
     2161 caccgtcgcg gccatcacga tcccgatcac catcggcacc gagaagccgt cccgagccag
     2221 gagccaggcg agcagggcat aggccggaat gccgattccg aagccggcca gggcggtccg
     2281 ccggtaggct aagtcgctcc agccgccgcg aaggaagccg ttcctcaggg ccaccatccc
     2341 gaacaggaaa taggccagag tctcccagcc gaagaacagg agccccacga tcggggcggc
     2401 cgcatgcgct ccgaggcggt aggcgacgag cgggccgtaa tcgccctgga acagcgccat
     2461 cttcccggcg agcgccggtc ccgcgatcac cccgaacttg gtgtcgaggt cccgccattc
     2521 cgtcaggatt tccgccgcag cgccgggagc cgaggcggcg tcctggaggt ggaaggcgcc
     2581 ggcggcgagg ccggcgaaga tcatcagctg gaccgtcacc agggcgatgc cccagcggag
     2641 gaggacggcg ggctccttgt tccggaagaa ccaggcgatc atcccgacga gggcgtagcc
     2701 gacgagaatg tcgccgaacc agatcagata gaggtggatg aggccgaaga cgagcagcca
     2761 gaacatgcgc cggaaatgga tcgaggccga gtcctgcccg cctgcctccg cccgctcgat
     2821 gaccagaagc atgcttgccc cgaacaggaa ggagaagagg ccccgcatct tgccgtcgac
     2881 gaaaatgaag ctgaccaccc aggacacgaa gtccgcgctc gactcccagc cgtaggcgag
     2941 cgggttcata taggcctgga agggcatggc gaaggcgacg atgttcatcg ccaatatgcc
     3001 catgaccgcg actccgcgga cgatgtccag agcggcgatt cgcggcggcg gctcggtagc
     3061 ggcggtcatc cggatccccc ttccgaacca tggtcgccgc gagtgatatc cgatcctgcc
     3121 gattgttcaa ccgtcgcgac cggccgctgc ggcggcgaag cgccgggcat gttccgtcgg
     3181 cgcggccgag gtcgttgctg cgcagcgcgc atttgaattc gtccgttctt cggtcatttg
     3241 gcccaatcct gacgggcttg gcgcagacga ctcctgcgct agtatttgcg cctttgccta
     3301 cactggaaaa tgaaaggcga cgagccgaac cggccgacgc ggcaggactg gtccttcatg
     3361 gctttctgtt cttgtacttt catcaggctg gtctcagcct gtgcggaagc ctatactcgc
     3421 acgcaaggga gatgaatgat ggggacattt actcgctgtt cgctttcggc cgccgtgact
     3481 ttcgctatgt tctccgctcc ggcacccgcc cagcctccac ttcccgacct gggcgtgccg
     3541 acgttgccga ccgtcaacgt cccggccggc cgggtggcgg tgcggttcat gggcacgtcc
     3601 acgatccttc tcgacgacgg caccacccaa atcctgatcg acggcttctt cagccggccg
     3661 ggcctgttgc tgctcccgat agcaccgaac aagccggtca tcgaatcggc gatggcgcgc
     3721 gccggaatcg gcaatcgcct caaggcggtg ctcgtcgcgc actcccatta cgatcatgcc
     3781 ctcgactcgg ccacgatcgc acggctcacc ggcgcgcggc tggtcgggtc cgcgtcgacc
     3841 gccaacgtcg gccgcggggg cggcttgccg aatagccaaa tccaagtcgt gcagcacggc
     3901 caggaactcg attatggcga tttccacgtt cgggtgtttc gctcccgtca tgcgccgccc
     3961 atggcctttc ccggcgagat cgtgactccg ctggcgcctc ccaaatgggt ttcgagctat
     4021 cgcgagggcg gcagctattc ctttctcgtc acgcaccacg gcttctcgat cctcatccac
     4081 ccgagcgccg acgccccaaa agatctttac aagaacattc gggcggacgt gatcttccag
     4141 ggcgtaggcg gacttagcaa acagtcgaac gacttcatct tgaggtatct gcaggagctc
     4201 cgatggcccg gagcccaagt cgtcattccc gtgcattggg acgatttcac gaagccactg
     4261 agttccggcc tgcggctgcc cccgaagtcc acggatgatg tcgcggcgat gatgaagggg
     4321 ctgcgcttct acgcccagat tcgaaatttc aggctcaggg ccatgccatt gttcgatccg
     4381 gtcgatatcg aagcggcgct cgcgccctga ggcggttcgg ggcggaagct aatcctccgt
     4441 cgctctggac tggagctgga tgtagttctg gaggcccatc cgggcgatca tctcgaactg
     4501 ggtttcgagg gagtcgacgt gggattcctc gttctctagg atttcggcga agaggtcgcg
     4561 gctgacatag tcgcgcaccg cctcgcaatg ctggatggcg cccttcagct gctcgaccgc
     4621 ctcgatctcg agctcgaggt cggagcggag cacctcctcg acggtctcgc cgatgcggag
     4681 gcggccgagg agctggaaat tgggcaggcc gtcgaggaag aggattcgct cggcgagccg
     4741 gtcggcatgc ttcatctcgt cgatcgactc gccgcgctcg aaggcggcga ggcgcttcac
     4801 gccccaattg tcgagcatcc ggtagtggag ccaatattgg ttggtggcgg tgagctcgtt
     4861 gcgcaaagcc tcgttgagaa gctcgatgac ccttatgtcg cccttcattc cggctctccc
     4921 ttcaggggcc gaacctgtag cggcttccaa cccgaaacgc atctaaaaaa cggcggaaaa
     4981 ctgcgaaaaa ctcgctgcgt ttgcgagtca atcgcggctc ggattcgggg gcgcgccgcg
     5041 gcgattgcgg atcattgtca ggaagcggcg ggccttggcc ggccgcggcg cggtcagcgc
     5101 gcggcctggg cggcggcgat gacctggcgc gcgaaagaaa agcactggcc gcagcgcggc
     5161 ttgcggccga agcggctgta agcgctgctc gggcaggaag cgccttcgag cgccgcctgg
     5221 cggacttcgg attcgcgaat cgcgttacag acgcaaacga tcatgtcatc gatgtagcga
     5281 taatgcgaag cgttcgcaat agagttcgtg cttcagcgcc ttcgggccat cagcttcagg
     5341 ccggaccata tggagtcgac cgcacagacc ttgacgtcga cgaggtcggt tacgggcagg
     5401 atggcatcgc gaattcggtc ctcaaggatg tccgtcggga cctttgaggc ttttttcggc
     5461 cagctcaccc agatgaaacc gcccggtgcc agcagcggcc gcagcgtggc gagcttcgtc
     5521 accatgtcgg catattgggt gacgaagatg tgcgcggcat cgatcggcat ct
//
LOCUS       DASWNU010000586         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_462942, whole genome shotgun sequence.
ACCESSION   DASWNU010000586 DASWNU010000000
VERSION     DASWNU010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462942"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VGB57_11690"
     CDS             complement(<1..1588)
                     /locus_tag="VGB57_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0132056.1"
                     /translation="MPEVFRVAIIGSGPAGLSAAARAARLGLSHVLLEKSDHLSDTIY
                     KYQKGKHVMATPGQLVLRSDMPFEAGKREEVLGAWDRLAADHEINVRLNAEVRSISGA
                     KGDFTLALANGEAIHAGNVILAIGTQGNPNLVRCPAEPGAVVQYQLDDPGEYVDEHIV
                     VIGAGDAGIENALGLAADPEQRNAVTIVNRSAEFATAKDANLKALLAAEAEGRMTVLR
                     ETTASAIGPGWIAFDTRDGELKARCDRVIARMGSAPPRAFVEACGIGFTSPDRLAYPK
                     LSPTFESSVDGIFVIGALAGYPLIKHCMNQGYDVVEFINGNLALKPADEPILQARFRG
                     LPVPRSVDAWLEMLRCNVAILHDMSPLQMREFMLDSEARFYRAGELVFERGDVGSSLF
                     GIASGSVAVQVSQADPSVTVPIATGSIFGEVGLISGRRRGATVRAAEDSILVEVPRMA
                     ALKLMSAVPAARRAITRISIERQLLQMFGSGLTADDLTEILETAEVRSVKAGEAILEE
                     GAEGRDIFVIRQGSMVVEKSI"
     gene            1667..>1904
                     /locus_tag="VGB57_11695"
     CDS             1667..>1904
                     /locus_tag="VGB57_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132057.1"
                     /translation="MTSAPQSPSRRPSAATIILILAAAIALASAAVAISRSVGGGPGG
                     GGDSPAAKDWRVVGWAYAQAGNAGEAAAAYRKAAA"
BASE COUNT          300 a          634 c          681 g          289 t
ORIGIN      
        1 cgatcgattt ctcgaccacc atcgagccct gacggatgac gaagatgtcg cggccttcgg
       61 cgccttcttc gaggatcgcc tcgcccgcct tgacgctcct cacctcggcg gtctcgagga
      121 tttcggtgag atcgtcggcg gtgaggcccg agccgaacat ctggaggagc tggcgctcga
      181 tcgagatccg ggtgatcgcc cggcgcgccg ccggcaccgc cgacatcagc ttcagggcgg
      241 ccatgcgggg cacctcgacc aggatcgaat cctcggcggc gcgcacggtg gcgccgcggc
      301 ggcggccgga gatcaggccg acctcgccga agatcgagcc cgtggcgatc gggacggtga
      361 ccgagggatc ggcctggctg acctggaccg cgacggagcc cgaagcgatt ccgaacaatg
      421 aggagccgac gtcgccccgt tcgaacacca actcccccgc ccgatagaag cgggcctcgc
      481 tatcgagcat gaactcgcgc atctggagcg gcgacatgtc gtggaggatg gcgacgttgc
      541 agcgaagcat ctcgagccag gcgtccaccg agcggggaac cggcaggcct cggaacctgg
      601 cctgcaggat cggctcgtcg gcgggcttca gagcgagatt gccgttgatg aactcgacga
      661 cgtcatagcc ctggttcatg caatgcttga tcaaaggata gccggcgagg gctccgatca
      721 cgaagatccc gtccaccgac gattcgaagg tcggcgagag cttggggtaa gcgagccggt
      781 cggggctggt gaacccgatc ccgcaggcct cgacgaaggc gcgcgggggc gccgagccca
      841 ttcgggcgat gacccggtcg cagcgcgcct tcagctcgcc gtcgcgggtg tcgaaagcga
      901 tccagccggg gccgatcgcc gaagccgtgg tctcgcgcag gaccgtcatc cggccttccg
      961 cctcggcggc gagcagggcc ttcagattgg cgtccttggc ggtggcgaat tcggcggagc
     1021 ggttgacgat ggtgacggcg ttgcgctgct cgggatcggc ggcgaggccg agcgcattct
     1081 cgatgccggc gtcgccggcg ccgatgacga cgatatgctc gtcgacatat tcgccggggt
     1141 cgtcgagctg atactggacg accgcgccgg gctccgcggg gcagcggacc aggttcgggt
     1201 tcccctgggt gccgatggcg aggatgacgt tcccggcgtg gatcgcctcg ccgttcgcca
     1261 gagcgagggt gaagtcgccc ttcgcgccgg agatcgacct gacctcggca ttgagcctga
     1321 cgttgatctc gtgatcggcg gccagccggt cccaggcgcc gagcacctcc tcgcgcttgc
     1381 cggcctcgaa gggcatgtcc gagcgaagca cgagctggcc cggagtggcc atgacgtgct
     1441 tgcccttctg atatttgtag atcgtgtcgg agaggtggtc cgacttctcg aggaggacgt
     1501 ggctgaggcc gagccgcgcg gctctcgcgg cggcgctgag gccggcgggg cccgagccga
     1561 tgatcgcgac gcggaaaact tccggcacgg cttgttccca cccctccagc cgcccccgca
     1621 cggcagccct tgacgttgac caaatcggcg cgaggatagc aagcccatga ccagcgcacc
     1681 ccaaagcccg tcccgccgcc cctccgcggc gacgatcatc ctgatcctcg cggcagcgat
     1741 cgcattggct tcggccgccg tcgccatatc gcgaagcgtc ggcggcggtc ccggcggcgg
     1801 cggcgacagc ccggcggcga aggactggcg ggtcgtcggc tgggcctatg cgcaggcggg
     1861 caatgccggc gaggccgccg ccgcctatcg caaggcggcg gcga
//
LOCUS       DASWNU010000587         3943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_360364, whole genome shotgun sequence.
ACCESSION   DASWNU010000587 DASWNU010000000
VERSION     DASWNU010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360364"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VGB57_11700"
     CDS             complement(<1..868)
                     /locus_tag="VGB57_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY0132058.1"
                     /translation="MPETSRRGFALGALAAGAALAARPSALLAAAEPALKPITGDARP
                     ITRGERIARVAKAQRLMRQAGLGSILIEPGASLTYFTGVRWGRSERLTAALIPARGRT
                     IVFTPFFEEPSVRESLDVPADVRTWVEHEDPLELVARALRDLKLASLPVGIEETVRFF
                     AVDGLRKSIPSVEIRNAAPVTRGCRMIKSAAETALMQKASDVTVAAYRHTFPRIEKGM
                     SPADIGAIMNGATVALGGKPEFALVLLGEASAYPHGTGKPQAVREGEVVLMDCGCTVH
                     GYQSDISRTFVFG"
     gene            complement(861..1484)
                     /locus_tag="VGB57_11705"
     CDS             complement(861..1484)
                     /locus_tag="VGB57_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0132059.1"
                     /translation="MIVVHHLENSRSQRILWLLEELGLPYEVRRYERDRKTMLAPPEL
                     RRVHPLGKSPVIEDEGRIVAETGAIVEYVVEKADNRLGPPAKRDDRLRYRHFLHYAEG
                     SMMPPLLAMLVVRRLGLLGRPARAPVQKMLDQHLDWLETELAGRSWFAGDAFTAADVM
                     MSFPLEASRHRAGLDEGRPNLIDWLERIHARPAYGAALRKGGPYAYA"
     gene            complement(1481..2314)
                     /locus_tag="VGB57_11710"
     CDS             complement(1481..2314)
                     /locus_tag="VGB57_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEY0132060.1"
                     /translation="METEGATRTDSPELRLIGAATDRQSSFLRGAALAPPLIRAALWS
                     DHGNMAAESGVELGIDILLGDAGDLPLSETDRDDALIAAAVADAAAGGAVPLVLGGDH
                     SITLPAIEALAAVHGAIDILHFDAHPDLYDDFEGNPRSHASPFARIMERGLARRLVQV
                     GIRTLNRHCREQAERFSVEIVEMRHFTPEAVPLPAGPLYISIDMDGFDPAVAPGVSHH
                     EPGGLTVRDVLAVLDRVRAPVVGADIVEYNPGRDINGVTAVLAAKMVREVAALCHRNN
                     A"
     gene            2638..3864
                     /locus_tag="VGB57_11715"
     CDS             2638..3864
                     /locus_tag="VGB57_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY0132061.1"
                     /translation="MLQPPRPDLTDARPGFREFVVLIAGLMSLNALAIDAMVPALPAI
                     GEALNVADENSRQLVVSLYVLGFGFAQIIYGPLSDRFGRKPVLVVSLILYVGFALACW
                     AAPSFTLLLAARMAQGGAAASTRVLVVSIVRDRYEGGRMARIMSLAFLVFLLVPMLAP
                     VFGQLTLLVASWRAIFFGLALGGAIMLLWTLIRLPETLNPEYRRPLNPKTIWDGTWQT
                     LSNRQSLGYTLAFTLMMASLMGYINSIQQIVFDIFRRPELIAATFAGVALPMAFTSWL
                     NSRIVERIGTRRVAHTGLALFTLSALIHLAIVLTIGENLWLFIAMQGLTMACFGLASA
                     NFGSLAMQPLGHVAGTASSVQGSIGTIGGALLGLLVGQSFNGTLVPMVGAFAAFGSLG
                     LLILLVTERGRLFGRG"
BASE COUNT          562 a         1358 c         1367 g          656 t
ORIGIN      
        1 cgccgaagac gaaggtgcgg gagatatcgg actggtagcc gtgaacggtg catccgcagt
       61 ccatcagcac cacctcccct tcgcggaccg cctgcggttt cccggtgccg tgcggatagg
      121 cgctcgcctc gccgagcagg acgagcgcga attcgggctt gccgcccagc gcgacggtgg
      181 cgccgttcat gatcgccccg atgtcggccg ggctcattcc cttttcgatg cgcgggaagg
      241 tgtggcgata ggcggcgacg gtcacgtccg acgccttctg catcagcgcc gtctcggccg
      301 cggacttgat catccggcag ccgcgggtga cgggcgcggc attgcgtatc tcgacgctcg
      361 ggatcgactt gcgcaggccg tcgacggcga agaagcggac ggtctcctcg atcccgaccg
      421 gcaggctcgc gagcttcagg tcgcgcagcg ccctggcgac cagctccagc gggtcctcat
      481 gctcgaccca ggtgcgcaca tcggcaggca cgtcgaggct ctcgcgcacc gacggctcct
      541 cgaagaaggg cgtgaagacg atcgtccggc cgcgggcggg gatgagggcc gcggtcagcc
      601 gctcgcttcg cccccagcgg actcctgtga agtaggtgag cgaggcgccg ggctcgatca
      661 ggatcgagcc gagcccggcc tggcgcatca ggcgctgcgc cttcgcgacc cgggcgatgc
      721 gctcgccgcg ggtgatcggc ctggcgtcgc cggtgatcgg cttcagcgcc ggctcggcgg
      781 ccgcgagcag cgccgacggg cgcgcggcca gcgcggctcc ggcggcgagc gcgcccagag
      841 cgaagccgcg gcggctcgtc tcaggcataa gcataaggtc cccctttcct gagcgccgcg
      901 ccgtaagcgg ggcgggcgtg gatccgctcg agccagtcga tgaggttcgg ccggccctcg
      961 tcgagcccgg cgcggtggcg cgacgcttcg agcgggaagc tcatcatcac gtcggcggcg
     1021 gtgaaggcgt cgccggcgaa ccaggaccgg ccggcaagct cggtctcgag ccagtcgaga
     1081 tgctggtcga gcatcttctg caccggcgcc cgcgccggcc gcccgagcag gccgaggcgg
     1141 cggacgacca gcatcgccag caggggcggc atcatcgagc cttcggcata atggaggaag
     1201 tgacggtagc gcagccggtc gtcgcgcttc gccggcgggc cgaggcgatt gtccgccttt
     1261 tcgaccacat attcgacgat tgcgccagtt tccgccacta tcctcccctc gtcctcgatc
     1321 accggcgact tgccgagcgg gtggacgcgc ctcagctccg gcggagcgag catggtcttg
     1381 cggtcgcgct cgtagcggcg cacctcatag ggcaggccga gctcctcgag cagccagagg
     1441 atgcgctggg aacggctgtt ctcgagatgg tggacgacga tcatgcgttg ttcctgtggc
     1501 acagggccgc aacttccctg accattttgg cggcgaggac tgcggtgact ccattgatgt
     1561 cgcggcccgg attatattcg acgatgtcgg cgccgaccac cggggcccgg acccggtcga
     1621 gcaccgccag gacgtcgcgc acggtgaggc cgcccggctc gtggtgcgag actcccggcg
     1681 cgacggccgg gtcgaagccg tccatgtcga tgctgatgta gagcgggccg gcaggcagcg
     1741 gcaccgcctc gggcgtgaaa tgccgcatct cgacgatctc gactgagaag cgctccgcct
     1801 gctcgcggca atggcggttc agggtccgga tcccgacctg gaccagccgc cgcgccaggc
     1861 cccgctccat gatccgggca aagggcgagg catgggagcg gggattgccc tcgaaatcgt
     1921 cgtacaggtc cggatgggcg tcgaaatgga ggatgtcgat cgctccgtgg acggcggcca
     1981 gggcctcgat cgccggcagg gtgatcgaat ggtcgccgcc gaggacgagc gggacggcgc
     2041 cgccggcggc cgcgtcggcg acggcggcgg cgatcagcgc gtcgtcccga tccgtttccg
     2101 agagaggcag gtcgccggca tcgccgagga ggatgtcgat tcccagctcg acccccgatt
     2161 cggcggccat gttgccgtga tcagaccaaa gggcggcccg gatcagcggc ggcgccagcg
     2221 ccgctccgcg caggaaggag gactggcggt cggtcgcggc gccgatcagc cgcagctccg
     2281 gactgtcggt cctggtcgcg ccttccgtct ccatgcgcgg cgtctgtgga gaggagcaaa
     2341 ggccttgtcc agcgggacac gaccaggagc cctcccgtca ccctgaacgc gtttcgcagc
     2401 tccatctcgc cgcccgttca gcgtcaggcc gtgggacgag cgctggcgcc tgcctcccgg
     2461 cgcctcggcc ccttcctgaa cgagacgacg cgacggcggc agactgagga gcggctcggc
     2521 cctgtatcct gaaacaagtt caggatgacg gcgggtgacg gtgctcgaaa tgggcgtaca
     2581 tcgcggaacc cgaagcgccc ggcatcgtct atgcgcgttc caccttcaag gatcgtcatg
     2641 cttcagccgc cccgccccga cctgaccgac gcccgcccgg gtttccgcga gttcgtcgtt
     2701 ctgatcgccg gcctgatgtc gctcaacgcg ctcgccatcg atgcgatggt gccggccctg
     2761 cccgcgatcg gcgaggctct gaacgtcgcc gacgagaaca gccgccagct cgtcgtctcg
     2821 ctctacgtgc tcggcttcgg cttcgcccag ataatctacg gcccgctttc cgaccggttc
     2881 gggcgcaagc cggtgctggt cgtcagcctg atcctctatg tcggcttcgc gctcgcctgc
     2941 tgggcggcgc cgagcttcac ccttctgctc gccgcccgga tggctcaggg cggggcggcg
     3001 gcctcgaccc gggtgctggt cgtctcgatc gtccgcgacc gttacgaggg cgggcggatg
     3061 gcgcggatca tgtcgctggc cttcctcgtc ttcctgctcg tgccgatgct tgcgccggtc
     3121 ttcggccagc tcaccctgct cgtcgcctcg tggcgcgcca tcttcttcgg cctggcgctg
     3181 ggcggagcga tcatgctgct gtggacgttg atccggctgc ccgagacgtt gaacccggaa
     3241 tacaggcggc cgctcaaccc gaagacgatc tgggacggga cctggcagac cttgagcaac
     3301 cgccagtcgc tcggctacac gctcgccttc accttgatga tggccagcct gatgggctac
     3361 atcaactcga tccagcagat cgtcttcgac atcttccgcc gacccgagct gatcgccgcg
     3421 accttcgccg gagtggccct gcccatggcc ttcacctcct ggctgaactc gcggatcgtc
     3481 gagcggatcg gaacccgtcg ggtcgcccat accggccttg ccctgttcac gttgtcggcg
     3541 ctgatccacc tcgccatcgt tctgacgatc ggcgagaacc tgtggctgtt catcgccatg
     3601 caggggctga ccatggcctg cttcggcctc gcctcggcca atttcggatc gctggcgatg
     3661 cagccgctgg gccatgtcgc gggcaccgcc tcgtccgtgc aggggtcgat cggaacgatc
     3721 ggcggagctt tgctcggcct cctcgtcggc cagagcttca atggcaccct cgtccccatg
     3781 gtcggcgcct tcgccgcctt cgggtcgctc ggcctgctga tcctgctggt aacggagcgg
     3841 gggcggctgt tcggtcgggg atgacgaagc gcggtgactg agctacccgg cgcagtgccc
     3901 gggccgaagg ccctataggg ttcgcacaat ggcacgatgg cac
//
LOCUS       DASWNU010000588         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_126874, whole genome shotgun sequence.
ACCESSION   DASWNU010000588 DASWNU010000000
VERSION     DASWNU010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126874"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..2360)
                     /locus_tag="VGB57_11720"
     CDS             complement(297..2360)
                     /locus_tag="VGB57_11720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY0132062.1"
                     /translation="MRNFSLAFTAALLLGTAAAPALAQGGHGGHSAAASGKARYGDFG
                     LDLGAMDRAVKPGDSFWHFVNGNWDKTTQIAADRTSAGPGVLLAEEAEGQVRSIVEDL
                     ARDPAKSGKIGQQVGDYYATWMDEAAIEKAGTAPLKPYLARIGAISSRTDLIRTFAEP
                     GFMSPVNVGILPDPADPTRYIAAAGQAGLGLPNRDYYLLEGEKYDTIRKAYRAYVVKI
                     QQLAGIADAEAKADRIIALETALAKVHWEPARQRDIKQIYNPMNRAQLAELAPQMEWP
                     VFLEAAGLGAVPTVIAAEKSAITETGKMLDSVPLSTWKDYLAFHFVSGHSFALPKAFD
                     LANFDFYSKTLRGVPQQRERWKRGVQMINGSLGEAVGRVYVERHYPAESNRQMSELIA
                     NLRAGLEARLAASPWMDAATKEQARAKLAAFDPRIGHPAKWIDYSSLEVKRGDLLGNV
                     VRAQKFGSDLQLSRLGKPVDRALWDMNPQEVNAYYNPLMNQITFPAAILQPPYFDPKA
                     DPAINYGSIGAIIGHEIGHGFDDQGRQFDATGRIRDWWSASSGEEFTKRAEKFGKQYD
                     QYEPIPGTRINGKLTMGENIGDLGGLEMAYAAYRRYVAQHGEPKVLNGLTGDQRFFIA
                     YGQSWRSKIREGALRERLLTDPHSPPEFRVNGVVRNVDAWYKAFNVKPGDKMYLPPEQ
                     RVSIW"
BASE COUNT          414 a          866 c          902 g          471 t
ORIGIN      
        1 ccgctggatt gcttcgtcgc tacgctcctc gcaatgacgg gttgtaacaa ttgacaccat
       61 aaacctgcgc ccgcggcacg aagccgtcac ctgccgggtc cagaactccg atcagaggct
      121 ccggccggca gcgttttcgc cccctcacgc gccggccggg ccgacccggt cgaaccagcc
      181 gtcatcccgg cgaagaccgg gacctcggga caagaagcct cggcgggtct ccaccagatc
      241 ccggcttccg ccgggatgac ggagatgtga aaaggggcgc cgaagcgccc cttcccttac
      301 cagatgctga cgcgctgttc cggcggcagg tacatcttgt cgcccggctt cacgttgaac
      361 gccttgtacc aggcgtcgac gttgcggacg actccgttga cccggaactc cggcgggctg
      421 tgcggatcgg tcaggagccg ctcgcggagc gccccttcgc ggatcttcga gcgccagctc
      481 tggccgtagg cgatgaagaa gcgctgatcg ccggtgaggc cgttcagaac cttgggctcg
      541 ccatgctggg cgacgtagcg gcgataggcg gcataggcca tctcgagccc gccgaggtcg
      601 ccgatattct cgcccatggt gagcttgccg ttgatcctgg tgcccgggat cggctcatat
      661 tggtcgtatt gcttgccgaa cttctcggcg cgcttggtga actcctcgcc cgagctcgcc
      721 gaccaccagt cgcggatccg cccggtggcg tcgaactgcc gcccctggtc gtcgaagccg
      781 tggccgatct cgtggccgat gatcgctccg atcgaaccgt agttgatcgc cggatccgcc
      841 ttcgggtcga aatagggcgg ctgaaggatc gcggccggga aggtgatctg gttcatcagc
      901 gggttgtaat aagcgttcac ctcctgcggg ttcatgtccc acagagcccg gtcgaccggc
      961 ttgccgagcc gcgaaagctg caggtcggaa ccgaatttct gggccctgac gacgttgccg
     1021 agcaggtcgc cgcgcttcac ctcgaggctc gaatagtcga tccacttggc cgggtggccg
     1081 atgcgcggat cgaaggcggc gagcttggcg cgcgcctgct ccttggtcgc cgcatccatc
     1141 cagggcgagg cggcaagacg tgcctctagg ccggcgcgca ggttcgcgat cagctccgac
     1201 atctggcggt tgctctccgc cggataatgg cgctcgacat agacccggcc gaccgcctcg
     1261 ccgagcgagc cgttgatcat ctggactccg cgcttccagc gctcgcgctg ctgcggaacg
     1321 ccgcgcaggg tcttcgaata gaagtcgaaa ttcgccaggt cgaaggcttt gggcagagcg
     1381 aaggagtggc cgctgacgaa atggaaggcg agataatcct tccaggtcga aaggggcacg
     1441 ctgtccagca tcttgccggt ctcggtgatc gcgctcttct cggcggcgat gacggtcggg
     1501 acggcgccca ggcccgcggc ctcgaggaag accggccatt ccatttgcgg cgccagctcg
     1561 gcgagctggg cgcggttcat cgggttgtag atctgcttga tgtcgcgctg gcgggccggc
     1621 tcccaatgaa ccttggcgag ggccgtctcg agcgcgatga tccggtccgc cttcgcttcc
     1681 gcgtcggcga tcccggcgag ctgctggatc ttcaccacat aggcgcgata ggccttgcgg
     1741 atcgtgtcgt atttctcgcc ttcgagcaga tagtagtcgc ggttggggag gccgaggccg
     1801 gcctggccgg cggcggcgat gtagcgggtc ggatcggccg gatcgggcag gatgccgaca
     1861 ttgaccgggc tcatgaagcc cggctccgcg aaggtgcgga tcaggtcggt acggctggag
     1921 atcgctccga tgcgggcgag atagggcttg agcggagcgg tgccggcttt ctcgatggcg
     1981 gcttcgtcca tccaggtggc gtaatagtcg ccgacctgct ggccgatctt gccggacttg
     2041 gcgggatccc gggcgagatc ctcgacgatc gaccgaacct ggccctcggc ctcttcggcg
     2101 agcagtacgc ccgggccggc gctggtgcgg tcggcggcga tctgcgtggt cttgtcccaa
     2161 ttgccgttga cgaaatgcca gaagctgtcg cccggcttca ccgcccggtc catcgcgccg
     2221 aggtcgaggc cgaaatcgcc gtagcgggcc ttgccgctcg ccgccgcgga gtggccgcca
     2281 tggccgccct gggcaagtgc cggcgccgcg gcggtgccga gcaaaagggc cgccgtgaag
     2341 gcgagactga aattgcgcat attagattcc ctcccgagcc gagattcggc tgtcgcgccg
     2401 acgactacca agcggcggcg cgacgggcaa tatcccgcaa cacaatggga taaatacgca
     2461 atttccgtca cctccgtcat cccggggaaa gccgggacct cgtggagacc ctccgggcgt
     2521 tccgttccga ggtcccggct atcgccggga tgacgaacag gcgagacgct tgcccttcat
     2581 cctcccgccc cgccgccacg gcgcctcggc gctggccgct ggactcgggc gctcggtcgc
     2641 gggtaaaggc gcg
//
LOCUS       DASWNU010000589         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_439171, whole genome shotgun sequence.
ACCESSION   DASWNU010000589 DASWNU010000000
VERSION     DASWNU010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439171"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..564)
                     /locus_tag="VGB57_11725"
     CDS             complement(73..564)
                     /locus_tag="VGB57_11725"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEY0132063.1"
                     /translation="MAKRSGSADLPLHGGRVPAWLGERMTRLGTVMAEAIIHHEGRDA
                     FLSRLAHPFWFQSFGAVMGMDWHSSGITTSVIGALKRGLNPRAGELGIRVCGGRGRHS
                     RATRRSWPPRATRPGSTDATWPARAGSSPRSTAPRSRTGSTSTCTASSSPTRADGRWS
                     SRA"
     gene            complement(868..>1694)
                     /locus_tag="VGB57_11730"
     CDS             complement(868..>1694)
                     /locus_tag="VGB57_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0132064.1"
                     /translation="ALVTGSLLRLNGARRSVAAEAGLIMASPSETTLIVLATAGAAQL
                     ISAETADFWQIVTAIGLTITPLLAKLGQMAARRVDVQTEDPAPSLGRGDEAAVVIIGF
                     GRVGRLVAEMLTAHGRRYLAVDSSIDTVAACRKEGIPIVYGDVARAEFMDRLNLGHAT
                     ALVLTMDDPVLSVRVTKKVRAWCPGLTIVARARDPAHAAELYRAGATDAVPETLESSL
                     QLSEAVLVDLGMAMGPVIASIHEKRAELRKEIMKLGQLEQEPVLERRRLRDALKGR"
BASE COUNT          260 a          569 c          595 g          270 t
ORIGIN      
        1 ggggcgaatc cacgaagctg gtcagccctt ccgacagcca atgatagcgc cgcgccgttc
       61 gccgctcgcc gttcatgccc tgctggacca ccgcccatct gccctcgtcg gtgacgaaga
      121 agccgtgcag gtagaggtcg aacccgtcct ggagcgcggc gctgtcgacc ttggcgacga
      181 gccggctcga gcgggccagg tcgcgtccgt cgaacccggc ctggtcgccc gcggcggcca
      241 gctccgccgg gttgctcgcg aatggcgccc gcgcccgccg cagaccctga tgccgagctc
      301 gcccgcccga gggttgaggc cgcgcttcag cgctccgatg acgctggtcg tgattcccga
      361 cgaatgccag tccatcccca tcaccgctcc gaacgactgg aaccagaaag ggtgggcgag
      421 ccgggagagg aaggcgtcgc ggccttcgtg atggattatg gcctcggcca tgaccgtccc
      481 gagccgggtc atccgctcgc cgagccaggc ggggacccgg ccgccgtgga gcggcaggtc
      541 ggcggagccg gatctcttgg ccatgactca tcctcccttg gaacggaggc ggaacgctag
      601 caggaatctg gcaccgtcac cagtcgtggc gatgtgtggc gatctgtggc gattcgctgc
      661 gattccaggc gacttggagc ggactttccg ctgccgacta tcgacgtgaa aacagtgatt
      721 tagctgacgg tggggcgccg ccgccggcgc ttttacgcag gttcgaggcc ggatttccgc
      781 gccttgcgca aataatcgag atgttcatga aacgttccac gtgaaacgtt gtagctctca
      841 ttttggcgcg gaagctcgag tgagccgtca ccgcccctta agcgcgtccc tcagccgccg
      901 ccgctcaagc accggttcct gctcgagctg gcccagcttc atgatctcct tcctgagctc
      961 ggcgcgcttc tcgtggatcg aggcgatgac cgggcccatg gccattccga gatcgaccag
     1021 caccgcctcg gaaagctgga gcgaggattc gagcgtttcg ggcaccgcgt cggtggcgcc
     1081 ggcgcggtag agctcggcgg cgtgggcagg atcgcgcgcc cgggcgacga tggtgaggcc
     1141 ggggcaccag gcgcggacct tcttggtcac cctcacgctc agcaccggat cgtccatggt
     1201 gaggacgagg gcggtggcgt ggccgaggtt gagccggtcc atgaactcgg cccgggcaac
     1261 gtcgccgtag acgatgggga tgccttcctt gcggcaggcg gcgaccgtgt cgatgctgga
     1321 atcgaccgcc aggtagcgcc gcccatgggc ggtcagcatc tccgcgacga gccggccgac
     1381 ccggccgaag ccgatgatca ccaccgcggc ctcgtcgccg cggccgagcg acggcgccgg
     1441 atcctcggtc tggacgtcga cccgccgcgc cgccatctgg ccgagcttgg cgagcagggg
     1501 cgtgatcgtc aggccgatgg cggtgacgat ctgccagaag tccgccgtct cggcgctgat
     1561 cagctgggcc gccccggcgg tggcgagaac gatgagggtg gtttcggacg gggaggccat
     1621 gatcagcccg gcctcggccg cgaccgagcg gcgcgcgccg ttgagccgaa gcagcgaccc
     1681 ggtgaccagc gcct
//
LOCUS       DASWNU010000590        11404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_49074, whole genome shotgun sequence.
ACCESSION   DASWNU010000590 DASWNU010000000
VERSION     DASWNU010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49074"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VGB57_11735"
     CDS             <1..1330
                     /locus_tag="VGB57_11735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0132065.1"
                     /translation="ALWSSMTAFSGFANSFAMLATARIGVGIGEASASPAAYSMISDW
                     FPKERRATALSIYSSGLYIGGALSLPIGGFVVARWNGAWPDTSLAPLGLAGWLAAFLA
                     VGLPGLLLALWVLTLREPQRGASDGLPQPIVRPNAWREFGGELVAILPPLTLLSVSRF
                     PGALRLNLIALAAVVVAAYGLYRLTGDGLQWIAYGLGVYAIFSWVQSLKFRDLPVYRL
                     IWGTPMVVTLAIAFGSISFVTYATSFWMPHYVEQNFYGGALEPARFIGGMKAKEEIGL
                     ILGWSAAFSAAAGVILGGYLSDVWRQRDPRGRLFVNMLSVILPIPFVAFLLTTDSLAA
                     FYWVNPIAHMLASAWVGAAVATLQDLVIPRMRATAGATYVLGTTMVGLALGPYYAGKV
                     SVISGDLATGIFALYVVPPFTLVALWIASRRIAELEATKVDRARESGEKI"
     gene            complement(1435..2025)
                     /locus_tag="VGB57_11740"
     CDS             complement(1435..2025)
                     /locus_tag="VGB57_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY0132066.1"
                     /translation="MKLAYWLIPGFSALALAACATEGGSGAGVDSAGAGDPGPPAAVS
                     TIGPPRLSADLRDALGRVRARATAESSGDSLRVRVEAVGMTPGAYGAHLHTTGRCDPP
                     GFTTAGPHWNPTDQKHGKDNPAGMHKGDLPNLIVGTDGRGSFEYTIPNAGLSGMLPNR
                     LIDADGAAVVIHARADDFRTDPSGNSGDRIACGVLG"
     gene            complement(2083..3396)
                     /locus_tag="VGB57_11745"
     CDS             complement(2083..3396)
                     /locus_tag="VGB57_11745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY0132067.1"
                     /translation="MRIAMVGTGYVGLVSGACFSDFGHDVVCIDKDEGKIEALRRGEM
                     PIFEPGLEDLVERNVRGGRLAFSTDLDAGVDGAEAVFIAVGTPSRRGDGHADLSYVFG
                     AAEEIARALRGPAVVVTKSTVPVGTGDEVERILRETAPEARAWVVSNPEFLREGAAIE
                     DFKRPDRIVVGTDDEEAAELMREIYRPLYLNKGPLMVTGRRTAELIKYAANAFLATKI
                     TFINELADLCEIVGGNVQDVARGIGLDNRIGSKFLHAGPGYGGSCFPKDTLALLKTAE
                     DYQAPLRIVEAVVKVNDARKRAMGRKVVQALGGEARGRTVALLGLTFKPNTDDMRDAP
                     SLSIVQALQDAGANVRGYDPEGMEQARPMMPDVEFFDSPYQAAAGADAVVLVTEWDVL
                     RALDLKRLAREMAQPVFVDLRSVYPPEDVEEAGLRWSGIGRPPRN"
     gene            complement(3780..4895)
                     /locus_tag="VGB57_11750"
     CDS             complement(3780..4895)
                     /locus_tag="VGB57_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0132068.1"
                     /translation="MRKLAIAMALSSTLLATPAFARDGAMYIGGEFGAMIVEDINVDV
                     GAVDNAITLNHDYGYDGGVFVGYDFGAFRLEAEAAYKKADLDDYQTTIRLPLEATNFA
                     ASRQGSGSTSVLSFMINGMLDFGDDDGISGFVGGGVGMARVKANNYRNFANATPFLDG
                     SDSKLAWQVFAGVRQAISDNIDVTVKYRFFNASKVELTAFNGAATEHRFRSHSLLGGL
                     TFNFGGAEPIPEPIPVPPEPTPIPPEPTPVPPPPPPACVAGPFMVFFDWDRDEITPQA
                     SAILDNAASAYQTCGQAQVMIAGHADKSGSDQYNVGLSQRRAANVRSYLAGRGIPDGV
                     MTTEAFGESRPLVETADGVREPQNRRVEITFGPGSGW"
     gene            complement(5099..5596)
                     /locus_tag="VGB57_11755"
     CDS             complement(5099..5596)
                     /locus_tag="VGB57_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEY0132069.1"
                     /translation="MTDTTPRFALPFILPGQAQKELFHNEALARIDLALHPAVEGPPA
                     AGPPPAPAQGECWIVAAAAGGDWSGQDDKLAMWTENGWRFLAPQPGTSVWNKAAAVPL
                     LWDGYQWGEGELAGAGLLVDGVRVVGPRQPGVASPSGGTIIDAEARVAINALTAALMS
                     HGLIE"
     gene            complement(5608..7785)
                     /locus_tag="VGB57_11760"
     CDS             complement(5608..7785)
                     /locus_tag="VGB57_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY0132070.1"
                     /translation="MATLLLTAAGTAIGGPIGGAVGAMIGQQIDGRLFAPKPRQGPRL
                     GELAVQTSSYGSQIPKLFGTMRVAGTVIWATDLKEERSKSGGGKGQPKTVNYSYSANF
                     AVALSGRPILAVRRIWADGKLLRGAAGDFKTPTGYRLHPGGEDQPVDPLIAAVEGSGQ
                     APAYRGIAYAVFEDFQLGDYGNRIPSLTFEVEADPGAMSISAVAAALGEGLATAGESA
                     ALAGYAAGGDSVRAAIATLAELEPLSLVDDGEGLVLAGGGGAPVLIAEDELGTSAPGA
                     GGRSEWLRRSGDSVPAEANLAYYDPARDYQTGLQRASRPGSALTSDRRAVAAALDAAT
                     AKSMAAGRLERLWAARRSARLHLGWSRLGLGAGRHVRIGGRPGLWRTARWTLDRMVLT
                     LELIGVPGSSPPAPGQASPGRPVDQPDLLHGPTSLLVLDLPHFAEEPPGRPRLLVAAA
                     GAGAGWRRADLIASFDGGTSWTAAGTTALPAVLGAALEVPAAAGSALIDERGRIEVEL
                     LNEAMWLESRSDPALADGANLALLGEELIQFGKVDSLGDRRFRLSRLLRGRRGTEWAA
                     GLHSPGEPFALIEAESLAVVEAPLGSLGGEARLLALGMGDPDGVLAVRTVAGESIRPP
                     SPVHLRAERLANGDLALTWVRRSRAGWVWLSGSDTPLGEESESYRLTLAGAGFARSVT
                     VAAPAYLYGAAEQAADGLAGPLTVEIVQLGTSAPSRPASLITN"
     gene            complement(7785..8189)
                     /locus_tag="VGB57_11765"
     CDS             complement(7785..8189)
                     /locus_tag="VGB57_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HEY0132071.1"
                     /translation="MKGVDIAARACALVGTRFRPQGRNPRDGLDCVGTAALACGIPSE
                     RVPRDYALRGQALAEIEHRLCDLGCRPVAGRVLELGDIAVCRTGPAQFHMGIITPLGF
                     VHADAALRRVVQRPYPIPWTLESAWRLAEEES"
     gene            complement(8193..9011)
                     /locus_tag="VGB57_11770"
     CDS             complement(8193..9011)
                     /locus_tag="VGB57_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEY0132072.1"
                     /translation="MPSFLERELTALAFCWRVERRDGICLGFTSHDRDLVRSGLVYRS
                     APGMLPSAVSVSDDFDASTLDVSGALTSDSIGEADLAAGRWDRAAVSLFLVDWEDPEG
                     ESLPLARGELGDVTVKANGFEAELRGAAALLERAVAEQTSPECRAELGDRRCRVDMAG
                     RVRVTRIESAPAEDVVVVAEAPGAPGAYGFGRLRWIGGSNSGLESAILASEGAQLRLQ
                     EAPPFAVAAGDLVEIAEGCDKSFATCGARFANAENFRGEPHLPGMDLLTRYPGA"
     gene            complement(9175..>11404)
                     /locus_tag="VGB57_11775"
     CDS             complement(9175..>11404)
                     /locus_tag="VGB57_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEY0132073.1"
                     /translation="FDARLWTVNFPRPMMASVVTTAPDSLRVDCVFYKADDLAGLIWE
                     SEDRFDHPLLGYETSRDFRGCRLRFRWRSGGLRALDAVDGPTLTIEGRDESGAARSWF
                     VRLWNYAEGSPEDSAVALDFADLAGGWTGEDPVWAGDVDRMFVSMVAPGYAHSDAPLA
                     APAQGWAELTGIACEGAGSVLAASDVLVPEHRLRMATGYDDLYHLTPARVLRNILQLG
                     YRGPINHYVGMSHYFRLEANSGGYYISLAGGVLNAPCGAWHSDFAVRAKALGFELIWS
                     LSYELLDQHVWGDWKQRAQDGSPALTGWSPPSTLLSPAHDGAMYYLRLVAQAFAAIAV
                     AAGLGMRFQVGEPWWWVMPDGRICLYDSAAVGAFAPAAIADVRAPLGAAQRATLDSAG
                     AVLAASTAALTAAVRDVAPGAETLLLAYLPTVLGAPDLKRANLPTGWASPAFDVLQLE
                     DYDWVTGGNAGASERGVAAAQARLGYPPDRQHYFAGFVLAAEDKAQWHSIAGAVEAAR
                     ARGASEVFVWALPQILRDGFTWFDLGDGDVDAFEDVRFPVALGREASVEPGFSTAVVT
                     GAGGGEQRNSDWADARLRFDAGPGVRGEADLHALIGFFRARRGAAQAFRFEDPFDNSS
                     KGMIGTPGAADQALGTGDGVRTGFALVKAYGGQVRRITRPVAGSVRVSVAGVERVSGW
                     TLGPLGMLSFEEPPAAGAAVRAGFRFDVPVRFAEDRLSVSRATFEAGEIASVPLIEVR
                     EG"
BASE COUNT         1775 a         4215 c         3687 g         1727 t
ORIGIN      
        1 cgcgctctgg tcgtcgatga ccgccttttc cggcttcgcc aacagcttcg ccatgctcgc
       61 cacggcccgg atcggagtcg gcatcggcga ggccagcgcc tcgccggccg cttactcgat
      121 gatctccgac tggttcccca aggagcggcg ggcgaccgcg ctctcgatct attcgagcgg
      181 cctctatatc ggcggggcgc tgagccttcc gatcggcggc ttcgtcgtcg cccgctggaa
      241 cggcgcctgg ccggatacct ctttggctcc cctcggcctc gccggctggc tggcggcgtt
      301 cctcgcggtc ggcctccccg gcctgctcct cgctctgtgg gtgctgaccc tgcgcgagcc
      361 gcagcgcggc gcctcggacg gccttccgca gccgatcgtc cgccccaatg cctggcgcga
      421 gttcggcggc gagctggtcg ccatcctgcc gcctctgacc ttgctcagcg tttcccgatt
      481 ccctggcgcc cttcggctca acctgatcgc cctcgcggcg gtcgtcgtcg ccgcctacgg
      541 cctttaccgg ctgaccggcg acggtctgca atggatcgcc tacgggctcg gggtctatgc
      601 catcttctcc tgggtccagt cgctcaagtt ccgcgatctt ccggtctacc ggctgatctg
      661 gggcacgccg atggtggtga ccctcgccat tgccttcgga agcatctcct tcgtcaccta
      721 tgcgaccagc ttctggatgc cccattacgt cgagcagaat ttctacggcg gagcgctgga
      781 gcccgcccgc ttcatcggtg ggatgaaagc gaaggaggaa atcggcctca tcctcggctg
      841 gtccgccgcc ttttccgccg cggccggagt gatcctcggc ggctacctct ccgacgtctg
      901 gcgccagcgc gaccccaggg ggcggctctt cgtcaacatg ctttcggtga tcctcccgat
      961 ccccttcgtc gccttcctcc tcaccaccga cagcctcgcc gctttctact gggtcaatcc
     1021 tatcgcccac atgctggcct cggcctgggt cggggccgcg gtcgcgacgc tccaggatct
     1081 ggtcatccct cggatgcgcg ccaccgcggg cgcgacctac gtactcggaa cgacgatggt
     1141 cgggctggcg ctcggccctt attatgccgg caaggtctcg gtcatctccg gcgacctcgc
     1201 caccggcatc ttcgccctct acgtcgtgcc gcccttcacc ctcgtggcgc tgtggatcgc
     1261 ctcgcgccgg atcgcggagc tcgaggcgac caaagtcgac cgcgcccggg aatcggggga
     1321 aaagatctag ggacaacttc cgtcattgcg aggagcgcag cgacgaagca atccagctac
     1381 ggcgggcgcg actggctcag cgctggattg cttcgctgcg ctcgcaatga cggcttaccc
     1441 caaaaccccg caggcgatcc ggtcgccgct gttgccggag gggtcggtcc ggaaatcgtc
     1501 ggccctggcg tggatgacca ccgccgcgcc gtccgcgtcg atcaggcggt tgggaagcat
     1561 tcccgacagg cccgcattgg ggatcgtata ttcgaagctt ccgcggccgt cggttccgac
     1621 gattaggttg ggcagatcgc ccttgtgcat tccggcggga ttgtccttgc cgtgcttctg
     1681 gtcggtcggg ttccagtgcg ggcccgcggt ggtgaagccg ggtgggtcgc agcggccggt
     1741 cgtatggaga tgcgcgccat aggcgccggg ggtcatgccc accgcctcta cccgcaccct
     1801 caggctgtcg cccgacgatt ccgccgtggc ccgggcgcgc accctgccca gggcgtcgcg
     1861 caggtcggcg ctcagccggg gaggaccgat ggtggagacc gcggccggcg gtccgggatc
     1921 gccggcaccg gccgaatcga cgccggcacc ggagccgccc tccgtggcgc aggcggcgag
     1981 cgcaagggcc gaaaagcccg ggatcaacca ataagcgagc ttcatcctgt tctcctcttc
     2041 acgggagtga acgatccggc ctgctcgcgg ttgctctcgc cctcagttgc gcggtggccg
     2101 gccgattccg gaccagcgca ggcccgcttc ctccacatcc tcggggggat agacgctgcg
     2161 caggtcgacg aagaccggct gcgccatctc gcgggcgagc cgcttcaggt ccagggcgcg
     2221 cagaacgtcc cattcggtga ccaggacgac ggcgtccgcc ccggccgccg cttggtaggg
     2281 gctgtcgaag aattcgacgt cgggcatcat cgggcgggcc tgctccatgc cttccggatc
     2341 gtagccgcgc acgttcgcgc cggcatcctg cagcgcctgc acgatgctga gcgaaggcgc
     2401 gtcgcgcatg tcgtccgtgt tgggcttgaa ggtcaggccg agcaaggcca ccgtccgccc
     2461 ccgagcctcc ccgccaagcg cctgcaccac cttccggccc atcgcgcgct tgcgggcgtc
     2521 gttcaccttc acgaccgctt cgacgatccg aagcggcgcc tgataatcct cggccgtctt
     2581 cagcagcgcg agcgtgtcct tggggaagca ggagccgcca tagccggggc cggcgtggag
     2641 gaacttggat ccgatccggt tgtcgagccc gatgccgcgg gcgacgtcct gcacgttgcc
     2701 gccgacgatc tcgcacaggt cggcgagctc attgatgaag gtgatcttgg tcgccaggaa
     2761 ggcattggcc gcatatttga tcagctcggc ggttcggcgg ccggtgacca tgagcggccc
     2821 cttgttgagg tagagcggcc ggtagatttc ccgcatcagc tccgccgctt cctcatcgtc
     2881 cgttccgacg acgatccggt cgggccgctt gaaatcctcg atcgcggcgc cttcgcgcag
     2941 gaattcgggg ttcgatacca cccaggcccg ggcttccggc gcggtttccc ggaggattcg
     3001 ctcgacctcg tcgccggtgc ccaccgggac ggtggatttg gtgacgacga cggcgggccc
     3061 gcgcaacgcc cgggcgatct cctcggcggc cccgaagaca tagctcaggt cggcatggcc
     3121 gtcgccgcgc cgcgacggag tgccgacagc gatgaacacg gcctcggccc cgtcgacccc
     3181 ggcatcgaga tccgtgctga aggcgagccg cccgccgcgc acgttgcgct cgacgagatc
     3241 ctcgagcccc ggctcgaaga tcggcatctc gccccgcctc agcgcctcga tcttgccttc
     3301 gtccttgtcg atgcagacga cgtcatggcc gaaatccgag aagcaggcgc cggagaccag
     3361 gccgacatag cccgtaccga ccatcgcaat gcgcattcaa ttgccttgca tcgtggagcg
     3421 agacgcccgc ttagcgcgcg tccgttcggc gcgacaaggg gtttgggaat aacctacatt
     3481 cggcaccggc cggagccgcc ctaaaaaccc tctcccgtcc ttcaccggag aggaggggcc
     3541 caggccgaag gcttgggagg tgagggtctt gctgcctccg gagtacagac ccgcaactca
     3601 ccgcttcgcg gcaggcccct cctgcgacgg gagagggaga agaaggactc cccgccgata
     3661 tcggcgaagc tctggggatg ccgccacggc ggaccgcgcc caaaaagaaa ggagccggcc
     3721 cgaaagccga ctccctcctt gttcctggcg ttgcgccgcc ggagcggcga acccccttct
     3781 taccagccgg agccgggtcc gaaggtgatc tccacgcgcc tgttctgagg ctcgcggaca
     3841 ccgtcggcgg tctcgaccag cggccggctc tcgccgaacg cttccgtggt catgacgccg
     3901 tcggggatgc cgcggccggc caggtacgaa cggacgttcg cggcacgacg ctgcgagaga
     3961 ccgacgttgt actggtccga acccgacttg tcggcgtggc cggcgatcat cacctgggcc
     4021 tggccgcagg tctgataagc ggaagccgcg ttgtcgagga tcgccgaagc ctgcggagtg
     4081 atctcgtcgc gatcccagtc gaagaagacc atgaacggac ccgcgacgca cgccggcggc
     4141 ggcggcgggg gaaccggggt cggctccggc ggaatcggag tcggctccgg cggaaccggg
     4201 atcggctcgg ggatcggctc ggcgccaccg aagttgaagg tcaggccgcc gagaaggctg
     4261 tgcgagcgga agcgatgctc ggtcgcagcg ccgttgaagg cagtcagctc gaccttcgag
     4321 gcgttgaaga agcgatactt caccgtcacg tcgatattgt cgctgatcgc ctggcgaacg
     4381 ccggcgaaga cctgccacgc cagcttcgaa tccgagccgt cgaggaaggg agtcgcgttg
     4441 gcgaagttgc gatagttgtt ggccttgacc cgcgccatgc cgacaccgcc gccgacgaag
     4501 ccggaaattc cgtcgtcgtc gccgaagtcc agcatgccgt tgatcataaa gctcagaacg
     4561 ctggtgctgc cgctaccttg gcggctggcc gcgaagttgg tggcttccag aggcagcctg
     4621 atcgtcgtct ggtaatcgtc gaggtcggcc ttcttatagg cggcctcggc ttcgagcctg
     4681 aaggcaccga agtcgtaacc gacgaacacg ccaccgtcat aaccataatc atggttgaga
     4741 gtgatggcgt tatccacggc accgacgtcg acattgatat cttcgacgat catcgccccg
     4801 aattcgccgc caatgtacat cgcgccatcg cgtgcaaagg ctggagtggc gagcagggtg
     4861 gatgaaagcg ccatagcgat ggcgagcttc cgcataaact ttcccctttc ttcttgcgtc
     4921 cctgtgggaa cgaccatgaa ctcaatacgg taagcggagt ttccgcgcaa gcacgcttat
     4981 ccccacaaat gttgccgaaa tgcctcagtg agtcggttag cgaatccttg aggcccctcg
     5041 tcagacggcc ggcaaacgac cctctgcccc cttggtttca cagccgcaag caaattgctc
     5101 actcgatcaa tccgtgtgac attaatgccg cagtcagggc gttgatcgcc acccttgctt
     5161 ccgcgtcgat tatcgttccg ccagaaggac ttgccactcc gggctggcgc ggaccgacca
     5221 ctctgacgcc gtcgaccagc agaccggcgc cggccagctc cccctccccc cactgatacc
     5281 cgtcccacag caggggcacg gccgcggcct tgttccagac gctcgtcccg ggctgtggcg
     5341 cgagaaagcg ccagccgttt tcggtccaca tcgcgagctt gtcgtcctgg ccgctccagt
     5401 cgccgccggc ggcggcggcg acgatccagc attccccctg cgccggagcc ggcggcggcc
     5461 cggcggccgg cgggccctcc acggcggggt ggagcgcgag atcgatccgg gccagcgcct
     5521 cgttgtggaa aagctccttc tgggcctggc ccggaaggat gaagggcaga gcgaagcggg
     5581 gcgtggtgtc ggtcatgatg atcctcctca attcgtgatc agggaagcgg gccgcgacgg
     5641 cgccgaggtt ccgagctgga cgatctccac ggtcaacgga cccgccagcc cgtcggcggc
     5701 ctgctccgcc gcgccgtaga gataggccgg cgccgcgacc gtcacgcttc tcgcgaatcc
     5761 cgctccggcc agggtcagcc ggtagctttc cgattcttcc ccgagcggcg tgtcgctgcc
     5821 gctcagccag acccacccgg cccggctccg cctgacccag gtcagggcca ggtcgccatt
     5881 ggccaggcgc tccgcccgca aatgcaccgg cgagggcggc cggatcgact ctcccgccac
     5941 cgtccggacc gcgagcacgc cgtcggggtc gcccatgccc aaggcgagca gcctcgcctc
     6001 gccgccgagc gaacccagcg gcgcctcgac gacggcaagg ctttccgcct cgatcaacgc
     6061 gaaaggctcg ccgggcgagt gcaggccggc ggcccattcc gtcccgcgcc gcccgcgcag
     6121 cagtcgcgag aggcggaagc gccgatcgcc gagcgaatcg accttgccga actggatcaa
     6181 ctcctcgccg agcaaagcca gattggcgcc gtcggcgagc gccgggtcgc ttcggctctc
     6241 cagccacatc gcctcgttca ggagctcgac ctcgattcgg ccgcgctcgt cgatcaaggc
     6301 cgagcccgcg gccgccggca cttccagcgc ggcgccgagg actgcgggga gagcggtcgt
     6361 tccggccgcc gtccagctcg ttccgccgtc gaagctcgcg atcaggtccg ccctgcgcca
     6421 gcccgcgccc gcaccggcgg ccgcgaccag caggcgcggc cggcccggcg gctcctcggc
     6481 gaaatggggc aggtcgagaa cgagcagcga agtcgggccg tggagcagat cgggctggtc
     6541 gacggggcgg ccggggctcg cctgccccgg cgccggaggc gagctccccg gcactccgat
     6601 cagctcgagc gtcagcacca tccggtcgag agtccagcgg gcggtcctcc agaggccggg
     6661 gcgcccgccg attcggacat ggcgtccggc cccgagcccg aggcgcgacc atccgagatg
     6721 aagcctggcc gagcgccgcg ccgcccacag ccgctcgagc cggcccgccg ccatcgactt
     6781 ggcggtcgcg gcgtcgaggg cggcggcgac ggcgcgtcgg tcgctggtca gggccgagcc
     6841 cggacggctc gcccgctgaa gcccggtctg atagtcgcgg gccggatcgt aataagcgag
     6901 gttcgcctcc gccggcaccg aatcgccgct tcgcctaagc cactcgctcc gtcctcccgc
     6961 cccgggcgcc gaggtcccca gctcgtcctc cgcgatgagg acgggggcac cgccgccgcc
     7021 ggcgagcaca aggccttcgc catcgtcgac cagcgacagc ggctcaagct cggcgagcgt
     7081 ggcgatcgcg gcccggacgc tgtcgccgcc ggccgcatag ccggccaggg ccgccgactc
     7141 ccccgccgtc gccagcccct cgccgagcgc ggccgccacg gcgcttatgc tcatcgcgcc
     7201 cggatccgcc tcgacctcga aggtcagcga cgggatccgg ttaccgtaat cgccgagctg
     7261 gaaatcctcg aacaccgcat aagcgatccc gcgataggcc ggtgcctggc cggagccctc
     7321 gaccgccgcg atcagcggat cgaccggctg gtcctcgccg ccgggatgaa gccggtagcc
     7381 ggtcggagtc ttgaaatccc cggccgcgcc gcgcagcagc ttgccgtccg cccagatccg
     7441 ccggaccgcg aggatcgggc gccccgacag ggccaccgcg aaattggccg aataactgta
     7501 attcaccgtc ttgggctggc ccttgccgcc gccgctcttc gatctttcct ccttgaggtc
     7561 ggtcgcccag atcaccgtcc cggcgacccg catcgtcccg aacagcttcg ggatctggct
     7621 tccgtagctc gacgtctgga cggcgagctc gccgagccgc ggtccctggc gcggcttggg
     7681 cgcgaacagc cgcccgtcta tctgctggcc gatcatcgcg ccgaccgcgc cgcctatcgg
     7741 ccctccgatc gcggttcccg ccgcggtgag caacaaagtc gccatcagct ttcctcctcg
     7801 gcaagacgcc aggcgctctc gagcgtccag ggaatgggat aaggccgctg caccacgcgc
     7861 cgaagcgcgg cgtcggcatg gacgaagccc aagggcgtga tgatccccat gtggaattgc
     7921 gcgggcccgg tccggcagac ggcgatgtcg ccgagctcca gcacgcggcc ggcgacgggc
     7981 cggcagccga ggtcgcaaag ccggtgctcg atctcggcca gggcctggcc gcgcagggcg
     8041 taatcgcgag ggacgcgttc gctcggaatg ccgcaggcga gcgcggcggt gccgacgcaa
     8101 tccagtccgt ccctcggatt ccgcccctgc ggccgaaagc gggtgcccac gagcgcgcag
     8161 gcccgtgcgg ctatgtccac gcctttcatt tctcacgctc ccggataacg agtgagaagg
     8221 tccatccccg gcagatgcgg ttcgccgcga aaattctcgg cattggcgaa gcgcgcgccg
     8281 caggtggcga agctcttgtc gcaaccctcg gcaatctcga cgaggtcgcc cgccgcgacc
     8341 gcgaagggcg gcgcctcctg aagccgaagc tgggcccctt ccgaggccag gatcgcgctt
     8401 tcgagcccgc tgttcgagcc gccgatccag cgcagccggc cgaacccata ggctccgggc
     8461 gcccccggcg cctcggccac gaccaccaca tcctcggcgg gcgccgactc gatccgggtc
     8521 acccgcaccc gcccggccat gtcgacccgg caccgccggt cgcccagctc ggcccggcat
     8581 tcgggcgagg tctgctcggc caccgcccgc tcgagcaggg ccgcggcgcc gcgcagttcg
     8641 gcctcgaagc cgttcgcctt cacggtcacg tcgccgagct cgccccgggc gagcggcagg
     8701 ctttccccct cgggatcctc ccagtcgacc aggaacaggg acaccgccgc ccggtcccag
     8761 cgcccggccg cgaggtcggc ctcgccgatc gaatcgctgg tcagggcccc gctgacgtcg
     8821 agggtagagg cgtcgaaatc gtccgagacc gagaccgcgg acggcagcat cccgggcgcc
     8881 gagcgataga cgagcccgct tctgacgagg tcgcggtcat ggctcgtgaa gcccaggcaa
     8941 attccgtccc gccgctccac ccgccagcaa aaggcgagcg cggtcagctc ccgctcgagg
     9001 aaggaaggca tggtgaaaac tcctctgtct aaaatcctcc ccgcgatttc gcggggaggg
     9061 ggaccatgcg aagcatggtg gaggggtctt gctgagtccg aagaacccct ccaccgcgct
     9121 tcgcgcggcc cccctccccg acaaatgtcg gggaggaatt ggagggcgac gggactaccc
     9181 ctcccgaacc tcgatcagag gcacggacgc gatctccccc gcctcgaacg tcgcccggct
     9241 aacgctgagc cgatcctcgg cgaagcgcac cggcacgtcg aaccggaatc cggccctcac
     9301 cgccgcgccc gcggccggcg gctcctcgaa gctcagcatc ccgagcggac ccagggtcca
     9361 cccactcacc cgctccaccc cggcaaccga aacccgaacg ctccccgcca ccggccgggt
     9421 gatccgccgc acctggccgc cataggcctt caccagcgcg aaacccgtcc gcaccccgtc
     9481 gcccgtcccc agcgcctggt ccgccgcccc cggcgttcca atcatcccct tcgagctgtt
     9541 gtcgaacgga tcctcgaacc ggaaggcttg cgccgcaccc cgccgcgccc ggaagaagcc
     9601 gatcaaagcg tgcaaatccg cctcgccgcg cacccccggc cccgcgtcga agcggagcct
     9661 ggcgtccgcc caatcgctgt tcctctgctc cccgccgccg gcgcccgtca ccaccgcggt
     9721 cgagaagccg ggctcgacgc tcgcctcccg cccgagcgcc accggaaatc gcacatcctc
     9781 gaaagcgtcc acgtccccgt ctcccaaatc gaaccaggtg aagccgtcgc gaaggatctg
     9841 cggcagcgcc cacacgaaca cctcgcttgc gccccgcgcc cgcgccgcct cgaccgcacc
     9901 ggcgatcgag tgccactgcg ccttgtcctc ggcggccagc acgaagccgg cgaagtaatg
     9961 ctgccggtcc ggcggataac cgagccgcgc ctgggccgcc gcgactccgc gctcgctcgc
    10021 ccccgcattg ccgccggtca cccagtcgta atcctcgagc tggaggacgt cgaacgccgg
    10081 gctcgcccag ccggtcggca aattggccct cttcaggtcc ggcgccccca gcacggtcgg
    10141 cagataggcg agcagcaggg tctccgcgcc gggcgccaca tcccgcaccg ccgccgtcag
    10201 cgccgctgtc gaagccgcaa gcaccgctcc ggccgagtcc agggtggcgc gctgcgccgc
    10261 cccgagcggc gcccgaacgt ccgcgatcgc cgccggagcg aaggccccca cggcggccga
    10321 gtcgtagagg cagatgcgcc cgtccggcat cacccaccac cagggctcgc cgacctggaa
    10381 ccgcatgcct aacccggccg cgaccgcgat cgcggcaaag gcctgggcga ccagccgcag
    10441 ataatacatc gccccgtcat gcgcgggcga cagaagggtc gagggcggcg accagccggt
    10501 cagcgccggc gacccgtcct gcgccctctg cttccagtcg ccccagacat gctggtcgag
    10561 caattcgtag gaaagcgacc agatgagctc gaagccgagc gccttcgccc gcaccgcgaa
    10621 gtccgaatgc caggccccgc agggcgcgtt cagcacccct ccggcgaggc tgatgtaata
    10681 gccgccgctg ttcgcctcga gccggaaata atggctcatc ccgacataat ggttgatcgg
    10741 cccgcgatag ccgagctgca ggatgttcct cagcaccctc gccggggtca gatgataaag
    10801 gtcgtcatag ccggtcgcca tccgcagccg gtgctccggc accagcacgt cgcttgcggc
    10861 caggaccgat ccggcgccct cgcaggcgat tccggtcagc tccgcccagc cttgggccgg
    10921 cgccgccagc ggggcgtccg aatgagcgta tccaggcgcc accatcgaca cgaacatccg
    10981 gtcgacatcg ccggcccaga ccggatcctc gccggtccag ccgccggcga gatcggcgaa
    11041 gtcgagcgcg accgccgaat cctccgggct cccctccgca taattccaaa gccggacgaa
    11101 ccagctccgc gccgcgccgc tttcgtcccg cccctcgatc gtaagggtcg gcccgtcgac
    11161 cgcgtcgagc gccctcaacc ccccgctgcg ccaccggaac cgaagcctgc agccgcgaaa
    11221 gtcgcggctc gtctcgtagc ccagcaacgg atggtcgaac cggtcctccg actcccagat
    11281 caggccggcg agatcgtccg ccttgtagaa gacgcagtcg acccgaagcg aatcgggcgc
    11341 ggtcgtcacc accgaagcca tcatcggcct cgggaagttg acggtccaca agcgcgcatc
    11401 gaag
//
LOCUS       DASWNU010000591         5047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_74631, whole genome shotgun sequence.
ACCESSION   DASWNU010000591 DASWNU010000000
VERSION     DASWNU010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74631"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VGB57_11780"
     CDS             <1..1249
                     /locus_tag="VGB57_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0132074.1"
                     /translation="ARQLMAQPHRLSRPPANAEKWFESMMTLAKGAANDAQWSTAYQI
                     ASQLDDAYPAGTDVSSRSYGERDEYTNLAWLAGTAAQKLGRHADASRMFDRYGRAAKS
                     SQTRTKGFYWAARSAFNAGQQAQSNSYLEQAAASPDQFYGQLALERLNRRVTPPSPSS
                     AVPSQAQRSAFAARPLIQAARALGDMGRWSDQSLFVRAIAEQAETESDRILAAQFGRT
                     IGRQDLGVWVAREARNKGQSFYDPAGFPEVTLPSAYSAYWGLAHGITRQESSFDRAAV
                     SHAGARGLMQLMPGTAREQAGKLGLPYDFGRLTSDPAYNVMLGSSYFERLLNQWGGQA
                     ALAVASYNAGAGNVNKWVRANGDPRGGTDIVTWIEAIPYSETRNYVQRVLENAVVYDA
                     IRAQRGGGRPNRLSYYLGKSGPG"
     gene            1349..1837
                     /gene="greB"
                     /locus_tag="VGB57_11785"
     CDS             1349..1837
                     /gene="greB"
                     /locus_tag="VGB57_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY0132075.1"
                     /translation="MTDRPNYITPDGYRRLREEYEALFALERPKLVETISWAAGNGDR
                     SENGDYIYGRKRLREIDRRLGWLSRRMKEAKVVDAAAQPERGKVYFGATVELADEGDN
                     RRIVTLVGEDETDASNGRIAWTSPIGRAIRGAAVGDLRRVTLPGGEREFEIVAIEYPA
                     AR"
     gene            complement(1929..2699)
                     /locus_tag="VGB57_11790"
     CDS             complement(1929..2699)
                     /locus_tag="VGB57_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEY0132076.1"
                     /translation="MIDLSDFESGARYRGNYSDDLAALQERLGRILVSHIVHKRRSLV
                     VFEGWDAAGKGGAIQRLTSGWDPRAFQVWPIKAPTQEEKARHFLWRFWNKLPGTGEIG
                     VFDRSWYGRVLVERVEGFASQAEWRRAYDEINEFEAQQSYDGTTIVKLFFHVTGETQD
                     ARLKARLDHPWKRWKVTAEDFRNRARRDDYLAAAQDMFECTNTRWGPWTAIDGNNKKA
                     ARIAALTAVAEALEAACPVKPPEADPEVERLAREALDE"
     gene            complement(2838..4655)
                     /gene="aspS"
                     /locus_tag="VGB57_11795"
     CDS             complement(2838..4655)
                     /gene="aspS"
                     /locus_tag="VGB57_11795"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY0132077.1"
                     /translation="MHAYRTHHCGELRASHAGERVKLSGWIHRKRDHGGVLFVDLRDH
                     YGLTQIVAKANSEPLRALEHLRAESVVTIVGEVVSRGSDAANPNLPTGEIEVSAEEVV
                     VQSVAEELPLPVAGEADYPEEIRLRYRFLDLRRERLHRNIVLRSQVISSIRRRMVEAG
                     FTEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLIMVAGFDRYFQIAP
                     CFRDEDGRADRSPGEFYQLDFEMSYVTQEDVFQALEPILAGVFEEFANGKKVTPAGEF
                     PRIPYKEAILKYGSDKPDLRNPILISDVSSHFQGSGFGLFARIVEGGGTVRAIPAPGT
                     ADKSRKFFDEMNDWARSEGHAGLGYVTQKGGELGGPIAKNHGDEGMRKLIAELGLGPD
                     DGIFFAAGKEAQAAKLAGLARTRVAEQLGLIGEDEYRFCWIVDFPMFEYDEEAKKVDF
                     SHNPFSMPQGELEALETKDPLDILAFQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAG
                     YSKADVDLNFSGMINAFKFGAPPHGGSAPGIDRIVMLLAGEPNIREVVLFPMNQRAED
                     LMMGAPGVVSAKQLRELNIRLVEPAGAAKKDPAKAVAPE"
     gene            4759..>5047
                     /locus_tag="VGB57_11800"
     CDS             4759..>5047
                     /locus_tag="VGB57_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY0132078.1"
                     /translation="MQIHPLITDSESLKALCERLARQPFVAVDTEFMRENTYWPDLCL
                     VQIGNTEEAAAVDPKAEGLDLTPMLDLLVDNDDVLKVFHAGGQDIEIIYNLT"
BASE COUNT          823 a         1651 c         1714 g          859 t
ORIGIN      
        1 cgcccgccag ctcatggccc agccgcaccg gctgtcgagg ccgccggcca atgcggagaa
       61 atggttcgag tcgatgatga ccctggccaa gggcgcggcc aacgacgcgc aatggtcgac
      121 ggcctatcag atcgcgtccc agctcgacga cgcctatccg gccggaaccg acgtcagctc
      181 aaggtcctat ggtgagcgcg acgaatatac caacctcgcc tggttggcgg gcaccgccgc
      241 gcagaagctc ggccgtcacg ccgacgcatc gcgaatgttc gaccgctacg gccgcgcggc
      301 aaagtcctcg cagacccgga cgaagggctt ctactgggcg gcccggtcgg cattcaacgc
      361 gggccagcag gcgcagtcga actcctacct cgagcaggcg gcggcgagcc cggatcagtt
      421 ttacggtcag ctcgctctgg agcggctcaa tcggcgggtg actcccccct ccccctcctc
      481 ggccgtgccg agccaggcgc agcgctcggc tttcgcggcc cggccgctga tccaggcggc
      541 ccgggcgctt ggcgacatgg gccgctggag cgaccagtcc ttgttcgtcc gcgccattgc
      601 cgagcaggcg gagacggaga gcgaccgcat cctcgccgcc cagttcggcc ggacgatcgg
      661 ccgccaggat ctcggcgtct gggtcgcccg cgaggcccgc aacaagggcc agagcttcta
      721 cgatccggcc ggcttcccgg aagtgaccct gccctccgct tattcggctt attgggggct
      781 ggcccatggc atcacccggc aggagagctc gttcgaccgc gccgccgtca gccatgccgg
      841 cgcccgcggg ctgatgcagc tgatgcccgg gacggcgcgc gagcaggccg ggaagctcgg
      901 cctcccttac gatttcggcc ggctgacctc ggaccccgcc tacaacgtca tgctcggctc
      961 cagctatttc gagcggcttc tcaaccagtg gggcggtcag gcggcgctgg cggtggcgag
     1021 ctacaatgca ggcgccggca acgtgaacaa gtgggtgcgc gccaatggcg acccgcgcgg
     1081 cggaaccgac atcgtcacct ggatcgaggc cattccctat tcggagaccc gcaactacgt
     1141 tcagcgggtg ctcgagaatg cggtggtcta cgacgccatc cgggcccagc gcggcggcgg
     1201 gcggccgaac cggctgtctt actatctggg caagagcggt cctggttgat cgcaggacgt
     1261 catgctgaac ttgtttcagc atccagtttc gagcagcctc gcggtggaga cctggaccct
     1321 gaaacaagtc cagggtgacg gaagagcgat gaccgaccgc cccaattaca tcacccctga
     1381 cggctaccgc cgcctccgcg aggaatatga ggccttgttc gcgctcgagc gcccgaagct
     1441 ggtcgagacc atctcctggg cggccggcaa tggcgaccgc tcggagaatg gcgattacat
     1501 ctacggccgc aagcgcctga gggagatcga ccggcgcctc ggctggctgt cgcggcggat
     1561 gaaggaggcc aaggtggtcg atgcggccgc gcagcccgag cggggcaagg tctatttcgg
     1621 agccaccgtc gagttggccg acgagggcga caaccggcgg atcgtcactc tcgtcggcga
     1681 ggacgagacc gacgcctcga acggccggat cgcctggacc tccccgatcg gccgggccat
     1741 ccgcggcgcg gcggtcggcg atctgcggcg ggtgactctg ccggggggcg agcgggagtt
     1801 cgagatcgtg gcgatcgagt atcccgcagc gcgttagccc gtcattgcga ggagcatagc
     1861 gacgaagcaa tccagccggg cctccgaggc ggcactggat tgcttcgcta cgctcgcaat
     1921 gacggggctt attcgtcgag cgcctcccgg gccagcctct ccacctccgg atcagcctcc
     1981 ggcggcttca ccgggcaggc cgcttcgagc gcctccgcca ccgccgtcag cgccgcgatc
     2041 cgggctgctt tcttgttgtt gccgtcgatc gcggtccacg ggccccagcg cgtgttcgtg
     2101 cattcgaaca tgtcttgggc cgccgccaga tagtcgtccc gccgggcgcg gttgcggaaa
     2161 tcctcggcgg tcaccttcca gcgcttccag ggatgatcga gccgggcctt caggcgggcg
     2221 tcctgggttt cgccggtgac gtggaagaac aatttgacga tcgtcgttcc gtcgtagctt
     2281 tgctgcgctt cgaactcgtt gatctcgtca taggcgcggc gccattcggc ttgcgaggcg
     2341 aagccctcga cccgctcgac caggacccgg ccgtaccagc tccggtcgaa gaccccgatc
     2401 tcgcccgtac cgggcagctt gttccagaag cgccagagga agtggcgggc cttctcctcc
     2461 tgcgtcggcg ccttgatcgg ccagacctgg aaggcgcgcg ggtcccagcc cgaggtcagg
     2521 cgctggatcg ccccgccctt cccggccgcg tcccagccct cgaagacgac caggcttcgc
     2581 ctcttgtgga cgatatggga gacgaggatc cgtccaagcc gctcctgaag cgcggcgagg
     2641 tcgtcggaat aattgccgcg gtagcgggcg ccggattcga aatcggagag gtcgatcatg
     2701 cgcttcctaa aatcctcccc gagaattctc ggggaggggg accatgcgaa gcatggtgga
     2761 ggggttcttc agagtcagca aggcccctcc accgcgcctc gcgcggtccc ctcccctgcg
     2821 aatgcagggg aggattttta ttccggcgcc accgccttcg ccggatcctt cttcgccgct
     2881 ccggcgggct cgacgaggcg gatgttgagc tccctgagct gcttcgcgct caccacgccg
     2941 ggagcgccca tcatcagatc ctcggcgcgc tggttcatcg ggaagagcac gacctcgcgg
     3001 atgttgggct cgccggcgag gagcatgacg atccggtcga ttccgggcgc cgagccgccg
     3061 tggggcggtg ctccgaactt gaaggcgttg atcatgccgg agaaattgag gtcgacatcc
     3121 gccttcgaat agccggcgat ctcgaacgcc ttgtacatga tgtcgggccg gtggttgcgg
     3181 atcgcgcccg agctcagctc gacgccgttg cagacgatgt cgtactggaa ggcgaggatg
     3241 tcgagcgggt ccttggtctc cagcgcttcg agctcgccct gcggcatcga gaaggggttg
     3301 tgcgagaagt cgaccttctt cgcctcctcg tcatattcga acatcggaaa gtcgacgatc
     3361 cagcagaagc gatactcgtc ctcgccgatc aggccgagct gctcggcgac ccgggtgcgc
     3421 gccaggccgg cgagcttcgc cgcctgggct tccttgccgg cggcgaagaa gatgccgtcg
     3481 tccggcccga ggccgagctc ggcgatcagc ttgcgcatgc cctcgtcgcc atggttcttg
     3541 gcaatggggc cgccgagctc gccgcctttc tgggtgacgt agcccaggcc ggcatggcct
     3601 tcggagcggg cccaatcgtt catctcgtcg aagaatttgc ggctcttgtc cgcggtgccc
     3661 ggcgccggga ttgcgcggac ggtgccgccg ccctcgacga tccgggcgaa gaggccgaag
     3721 cccgagccct ggaagtggct cgagacgtcg gagatgagga tgggattgcg aaggtccggc
     3781 ttgtcggagc cgtatttcag aatcgcctcc ttgtagggga ttcgcgggaa ctcgccggcg
     3841 ggggtgacct tcttgccgtt cgcgaactcc tcgaagaccc cggcgaggat cggctccagc
     3901 gcctggaaga cgtcctcctg ggtgacgtag ctcatctcga aatcgagctg gtagaattcg
     3961 ccgggcgagc ggtcggcgcg gccgtcctcg tcgcggaagc agggggcgat ctggaagtag
     4021 cggtcgaagc cggcgaccat gatcagctgc ttgaacatct gcggcgcctg cgggagcgcg
     4081 tagaatttgc cgggatgcac ccggctcggg acgagatagt cgcgcgcgcc ctcggggctc
     4141 gaggcggtga ggatcggggt ctggaactcg gtgaagccgg cctcgaccat tcggcggcgg
     4201 atcgaggaga tgacctggct tcgaagaacg atgttgcggt ggagacgctc gcggcgaagg
     4261 tcgaggaagc ggtagcggag gcggatctcc tccggataat ccgcctcgcc cgcgaccggg
     4321 agcggaagct cctccgccac ggactggacg acgacctcct cggccgaaac ctcgatctcg
     4381 ccggtcggaa ggttcgggtt ggccgcgtcc gagccgcgcg agacgacttc gccgacgatc
     4441 gtcaccaccg actcggcgcg aagatgctcg agcgctcgaa gcggctcgct gttcgccttg
     4501 gcgacgatct gggtgagacc gtaatggtcc cgaagatcga cgaacaggac tccgccatgg
     4561 tcgcgcttgc ggtggatcca gcccgaaagc ttcacccgct ccccggcgtg ggaggcgcgg
     4621 agctcgccgc aatggtgggt gcgataggcg tgcataaagg cgctttctga gcttttttca
     4681 cggattgtga ggatgggggc gcttcgccac gcagccttgc tttgtcaagc ggggaggccg
     4741 ccgctatgag ccctgccgat gcagatccac ccgctgatta ccgattccga gtcgctgaag
     4801 gcattgtgcg agcgtctggc gaggcagccc ttcgtcgcgg tcgacaccga attcatgcgc
     4861 gagaacacct attggcccga cctctgcctg gtgcagatcg gcaataccga agaggccgcg
     4921 gcggtcgatc cgaaggccga agggctggac ctcacgccca tgctcgacct gctggtcgac
     4981 aatgacgacg tgctcaaggt cttccatgcc ggcgggcagg atatcgagat catctacaat
     5041 ctaaccg
//
LOCUS       DASWNU010000592         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_232009, whole genome shotgun sequence.
ACCESSION   DASWNU010000592 DASWNU010000000
VERSION     DASWNU010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232009"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VGB57_11805"
     CDS             complement(<1..1227)
                     /locus_tag="VGB57_11805"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0132079.1"
                     /translation="MSENARFAFEVEAAEDGEGTRIEVRSAGRPDVRAYRVAGGDQGD
                     YAAFYRELADDWGTRVPHAFAQPAGAPEAEVEWRPLLTDNVSPKILAGYGDPAVLKTD
                     EGYVLVATSNDAPDAFPILRSDDLQHWHHEGFVFPEGHAPEWTAQGRKVADFWAPEMA
                     RVGDEYWLVYSARKISNALAIGLARSASPFGPWRDLGRPLIAGHAVNTTGTPDDPGKP
                     QLSGGVIDAHIFIDEDGQPFLYWKADTNGVWPRPLATLLRARPELIDRLFEREEDRRT
                     AAFAAAIVAWANGRRPMERFFLMQPLIEAALANWRPVRDALEQCGVAGAILEAMRTPI
                     YAQRIALDGESFLGEPTVVLANDQDWEGHLIEGPWVTRQQGRYWLFYAGNDFSTPAYG
                     IGVAAADHPLGPYVKQP"
     gene            1306..>1975
                     /locus_tag="VGB57_11810"
     CDS             1306..>1975
                     /locus_tag="VGB57_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0132080.1"
                     /translation="MATAAKTEEQVYDTASIMGGLYGDGIIALKGAFPRPWAERMRED
                     IVSLFEEAQKVPGGALPRGPQRWYVEVHPERIRGFVDIATHPWFVAVCESVLGPDYKI
                     VEIGFDIPFPGAADQPWHRDFPAPEATLKGRRLNSLAFNLSAVDTRPEHGAFEIAPGT
                     QWDDLSGCKDGMFPPREYWPRYISRSQARLAQMGDISARSALTVHRGTANRSDEWRPV
                     LVVGV"
BASE COUNT          313 a          636 c          713 g          313 t
ORIGIN      
        1 gggctgcttg acgtaggggc cgagcggatg gtccgcggcc gcgaccccga tgccgtaggc
       61 gggagtcgag aaatcgttgc cggcgtagaa gagccagtag cggccctgct ggcgggtgac
      121 ccaggggcct tcgatgagat ggccttccca atcctgatcg ttggcgagga ccacggtggg
      181 ctcgccgagg aaggactcgc cgtccagggc gatgcgctgg gcataaatgg gggtgcgcat
      241 cgcctccagg atcgctccgg cgactccgca ttgctcgagc gcgtcgcgga ccggccgcca
      301 gttggcgagc gccgcctcga tcagcggctg catcaggaag aagcgctcca tcggccggcg
      361 gccgttcgcc caggccacga tcgcggcggc gaaggcggcg gtgcgccgat cctcctcgcg
      421 ctcgaaaagg cggtcgatca gctccggccg ggcgcgcagc aaagtggcga gcgggcgcgg
      481 ccagacgccg ttggtgtcgg ccttccaata gaggaaaggc tggccgtcct cgtcgatgaa
      541 tatgtgcgcg tcgatcactc ccccgctcag ctggggcttg ccgggatcgt cgggcgtgcc
      601 ggtggtgttg acggcgtggc cggcgatgag ggggcggccg agatcgcgcc aggggccgaa
      661 ggggctcgcg cttctggcga ggccgatggc gagggcgttc gagattttgc gggcggagta
      721 gaccagccaa tattcgtcgc cgacgcgggc catttcgggc gcccagaaat cggcgacctt
      781 gcgcccctgt gccgtccact ccggggcgtg gccttcgggg aagacgaaac cttcatggtg
      841 ccaatgttgc aggtcgtccg agcgcaggat gggaaaggcg tcgggcgcgt cgttcgaggt
      901 ggcgacgagg acgtagccct cgtccgtctt gagcacggcc ggatcgccgt aaccggctag
      961 gatcttcggc gagacgttgt cggtgagcag cgggcgccac tcgacctccg cctcgggcgc
     1021 cccggcgggc tgggcgaaag cgtgggggac ccttgtaccc cagtcgtcgg ccagctcgcg
     1081 gtagaaagcg gcataatcgc cctgatcgcc gccggcgacg cggtaagcgc ggacgtccgg
     1141 ccggcccgcg gagcgcacct cgatccgggt tccctcgccg tcttccgctg cctccacctc
     1201 gaaggcgaat cgtgcatttt ccgacatcgg cgggccgtta gccgcctcgc aatcatcaga
     1261 caaatacatt aatttgcaaa tcgcttcaga cgagatggat tcgagatggc gacggcggcg
     1321 aagacggaag agcaggttta cgacacggcc tccatcatgg gcggccttta cggcgacggc
     1381 atcatcgccc tgaagggcgc cttcccgagg ccgtgggcgg agcggatgcg ggaggatatc
     1441 gtgtccctgt tcgaggaggc gcagaaggtg cccgggggcg ccctgccgcg cgggcctcag
     1501 cgctggtacg tcgaggtcca tcccgagcgg atcaggggct tcgtcgacat cgccacccat
     1561 ccctggttcg tcgcggtgtg cgagtcggtg cttgggcccg actacaagat cgtcgaaatc
     1621 gggttcgaca tccccttccc cggcgccgcc gaccagccct ggcaccgcga cttcccggcg
     1681 ccggaagcga ctctgaaagg ccggcgcctc aactcgctgg ccttcaacct cagcgccgtc
     1741 gacaccaggc cggagcacgg cgccttcgag atcgcccccg gcacccagtg ggacgatctg
     1801 agcggctgca aggacgggat gtttccgccc agggaatatt ggccgcgcta tatctcgcga
     1861 agccaggcga ggctcgccca gatgggggac atctcggccc gctcggcgct gaccgtgcat
     1921 cgcggaaccg ccaaccgctc ggacgaatgg cggccggtgc tggtggtggg agtcg
//
LOCUS       DASWNU010000593         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_385387, whole genome shotgun sequence.
ACCESSION   DASWNU010000593 DASWNU010000000
VERSION     DASWNU010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385387"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VGB57_11815"
     CDS             <1..1133
                     /locus_tag="VGB57_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY0132081.1"
                     /translation="LGKTIGGEPVIADLARMPHLLVAGTTGSGKSVAINTMILSLVYR
                     LTPAECRLIMVDPKMLELSVYDGIPHLLTPVVTDPKKAVVALKWAVREMEDRYRKMSK
                     LGVRNIDGFNARVVEAKAKAETITRTVQTGFDRETGEAIYEDEVMDLDPLPYIVVIVD
                     EMADLMMVAGKEIEGAIQRLAQMARAAGIHVILATQRPSVDVITGTIKANFPTRISFQ
                     VTSKIDSRTILGEMGAEQLLGQGDMLYMAGGGRITRVHGPFCSDEEVEKVVAHLKRQG
                     RPQYLEAVTAEEDEGGAAGADAPVFDQGEFGAPSGDLYDQAVAVVLRHKKASTSYIQR
                     RLQIGYNRAASIMERMEKEGIVGPANHAGKREILLETAGEED"
     gene            1234..>1648
                     /locus_tag="VGB57_11820"
     CDS             1234..>1648
                     /locus_tag="VGB57_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132082.1"
                     /translation="MPRRSSTSLALGLVLSASWFGPAAAQNPFEFLFGSSSAPAKTAA
                     PATTPASVSSGMPATAILPPRRPASFAEDPTTTQALGRSAAPAPETRPIVTAAAPTAT
                     PAAAPGGAPLGEKQIVDRANAYFNAITSLVGDFVQV"
BASE COUNT          289 a          559 c          554 g          246 t
ORIGIN      
        1 gcctgggcaa gaccatcggg ggcgagccgg tcatcgccga tctcgcccgc atgccgcacc
       61 tgctggtggc cggcaccacg ggctcgggca agtcggtggc gatcaacacc atgatcctct
      121 ctctcgtcta ccgcctgacc ccggccgagt gccgcctgat catggtcgac cctaagatgc
      181 tggagctctc cgtctatgac ggcatcccgc acctgctcac ccctgtcgtg accgacccca
      241 agaaggccgt ggtggcgctc aaatgggccg tgcgcgagat ggaggaccgc taccgcaaga
      301 tgtccaagct cggcgtgcgc aacatcgacg gcttcaacgc ccgggtggtg gaggcgaaag
      361 cgaaggctga gaccatcacc cgcacggtgc agaccggctt cgaccgcgag accggcgagg
      421 cgatctacga ggatgaggtg atggatctcg atccgttgcc gtacatcgtg gtgatcgtcg
      481 acgagatggc cgacctgatg atggtggccg gcaaggagat cgagggcgcc atccagcgcc
      541 tggctcagat ggccagggct gccggcattc acgtgatcct ggccacccag cgtccatcgg
      601 tggacgtgat caccggcacg atcaaggcga acttcccgac ccggatctcg ttccaggtca
      661 cgtccaagat cgacagccgc acgatcttag gggaaatggg cgccgagcag ctcttagggc
      721 aaggcgacat gctgtacatg gccggcggcg ggcgcatcac ccgcgtgcac gggccgttct
      781 gctcggacga ggaggtcgag aaggtggtgg cgcatctcaa gcgccaggga aggccgcagt
      841 atcttgaggc ggtcacggcc gaggaggacg agggcggagc ggccggggcg gatgcgccgg
      901 tgttcgacca gggcgagttc ggggcgccta gcggggatct gtacgaccag gcggtggcgg
      961 tggtgctcag acacaagaag gcctcgacga gctacatcca gcgccggctg cagatcggct
     1021 ataaccgggc tgcctcgatc atggagcgca tggagaagga aggcatcgtc ggtcccgcca
     1081 accacgccgg caagcgcgag atcctgctcg agaccgccgg cgaggaggac tagcgcaaac
     1141 ccgtgcaacg ccgttcaccc cggctacgtt gtcattgaat cgccatagcg tccgcgtagc
     1201 ggccctcgca tgatcagaaa gtcgaaaccg atgatgcccc gccgatcctc gacgtccttg
     1261 gccctcggcc ttgttctgag cgcgagctgg ttcggcccgg ccgccgccca aaacccgttc
     1321 gagttcctgt tcggaagctc ttccgcgccc gccaagacgg ccgcgccggc gacgacaccg
     1381 gcctcggtct cgtcggggat gccggcgacc gccatcctgc cgccgaggcg ccccgcgtca
     1441 ttcgctgagg atccgacgac cacgcaggcg ctcggccgct cggccgctcc ggccccggag
     1501 acgcggccca tcgtaacggc ggcggccccc accgccacgc cggccgcggc cccgggcggg
     1561 gctcccctcg gcgagaagca gatcgtcgac cgggccaacg cctacttcaa tgccatcacg
     1621 tcgctcgtcg gcgacttcgt gcaggtcg
//
LOCUS       DASWNU010000594         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_232095, whole genome shotgun sequence.
ACCESSION   DASWNU010000594 DASWNU010000000
VERSION     DASWNU010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232095"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VGB57_11825"
     CDS             <1..171
                     /locus_tag="VGB57_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0132083.1"
                     /translation="HHQGKVVSRTELIEHIYDQDFDRDSNTIEVFVTRIRKKLGQDVI
                     TTIRGLGYSLED"
     gene            344..1750
                     /locus_tag="VGB57_11830"
     CDS             344..1750
                     /locus_tag="VGB57_11830"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0132084.1"
                     /translation="MKRPRLPRLFRRFPRAARTGSLTRRMIGVSALWILVLLGVGGYT
                     LDRVLTSAITRNFDAQLEYVLTALIASAEVGTEGEALLNRPLADQRFLEPYSGLYFQI
                     SAVANKPVGDPADIDLPSRSLWDRKLKVANAHNDVGLHIYDSRELEGETLRVLERDIQ
                     LPGSETRWRFQVAQSREDLDDQISVLRRTLLRSFGILGLGLLVLAALQALYGLWPLRR
                     VRRAIAAIRSGEKSRIEERFPTEIEPLTEELNALLEHNEKQAEEARRHAGNLAHALKT
                     PLTVITNAATARAPDLADTVCREATTMRRQVDHHLARARAIGRRSSAQARSQVWPSLE
                     AVERAVTRLHDNVTVDLAGDKAASVRVERQDLDEMLGNLIENAAKYGGGRVFVTVKAT
                     PECVEIEVEDDGRGIPEGERQSIFDRGARLDTGKPGTGLGLAIVRDVAQIYGGSISLE
                     ESEDLGGLLVRLRLPAAS"
BASE COUNT          303 a          684 c          555 g          267 t
ORIGIN      
        1 caccaccagg gcaaggtcgt ctcgcgcacc gaactgatcg agcacattta cgaccaggat
       61 ttcgatcgcg attcgaacac gatcgaagtg ttcgtcaccc gcatccgcaa gaagctcggg
      121 caggacgtga tcaccaccat ccgcggcctc ggctattcgc tggaagattg agggctcgcg
      181 tgtcccccgt catcccggcg aaagccggga cctcaaggag gaaaggcgcc ctaccgaccc
      241 gaggtcccgg ctttcgccgg gatgacgatg ccgtgagtcg ctcgcgaata cccgctacac
      301 cctgcgttca tggctcccgg atcaagtccg ggatgacgat cccatgaaac gcccccgcct
      361 cccccgactc ttccgccgct tcccgcgcgc cgcgcgcacc ggctcgctga cccggcgcat
      421 gatcggcgtg tcggcgctct ggatcctggt cctgctcggg gtcggcggct atacgctcga
      481 ccgggtcctc acctcggcca tcacccgcaa tttcgacgcc cagctcgaat atgtgctgac
      541 cgccctcatc gcctcggccg aggtcggcac cgagggcgag gcccttctca accgcccgct
      601 cgccgaccag cgcttcctcg aaccttactc gggcctctat ttccagatca gcgccgtcgc
      661 caacaagccg gtcggagacc cggccgacat cgaccttccc tcgcgctcgc tgtgggaccg
      721 caagctcaag gtcgccaacg cccataacga cgtcggcctc cacatctacg acagccgcga
      781 gctcgagggg gagaccttgc gggtgctcga gcgcgatatc cagcttcctg ggtcggagac
      841 gcgctggcgc ttccaggtcg cccagagccg ggaggacctc gacgaccaga taagcgtgct
      901 caggcggacg cttctgcgct cgttcggaat cctcggcctc ggcctgctcg tcctcgccgc
      961 cctccaggcc ctgtacggcc tctggccgct gcgccgagtg cgccgggcga tcgccgccat
     1021 ccgctccggc gagaagtccc ggatcgagga gcgcttcccg accgagatcg agcccctgac
     1081 ggaggaattg aacgccctcc tcgagcataa tgagaagcag gccgaggagg cccggcgcca
     1141 cgccggcaac ctcgcccacg ccctgaagac cccgctcacc gtcatcacca acgccgccac
     1201 cgcccgtgcg cccgacctcg ccgacaccgt ctgccgggag gcgacgacga tgcgccgcca
     1261 ggtcgaccac cacctcgccc gcgcccgggc gatcggccgc cgctcctccg cccaggcgcg
     1321 ctcccaggtc tggccgtcgc tggaagcggt cgagcgcgcc gtcacccggc tccacgacaa
     1381 cgtcaccgtc gacctcgccg gcgacaaggc cgcgtcggtc cgggtcgagc gccaggatct
     1441 ggacgagatg ctcggcaacc tgatcgagaa tgccgccaaa tatggcggcg gccgggtctt
     1501 cgtcaccgtc aaggccaccc ccgaatgcgt cgagatcgag gtcgaggacg acggccgcgg
     1561 aatccccgag ggagagcgcc agagcatctt cgaccgcggc gcccgcctcg acaccggcaa
     1621 gcccggcacc ggcctcggcc tcgccatcgt ccgggacgtc gcccagatct acggcggctc
     1681 gatcagcctc gaggagagcg aggatctggg cggattgctg gtgcggctga ggttgccggc
     1741 cgcctcctga cggttgtacg aggccggcaa aactgcgagc atccgcccta gatcaggggg
     1801 gatcatcat
//
LOCUS       DASWNU010000595         7160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_385508, whole genome shotgun sequence.
ACCESSION   DASWNU010000595 DASWNU010000000
VERSION     DASWNU010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385508"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1503)
                     /locus_tag="VGB57_11835"
     CDS             complement(247..1503)
                     /locus_tag="VGB57_11835"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY0132085.1"
                     /translation="MRRYLLLAAGAALLAASPASADTVCEWIDYAQTVGTAAAPPADA
                     PRTGEHDRAQTQVAIAMFEALNAIDRRFESYLGMAAAAPGASQDAAAATAAYQVLIAH
                     YPGQKAGLDDSYDVTMLGIPDSAARERGKAVGEAAAKLALAAGGFDPARKPVPYRPRT
                     SAGMWTATQLPVYQPYAFTFKPWILPTYDSVRPGPPPALNSERWARDFEEIKRLGSKT
                     SSARTPQQSLMARYRITPNMTPTLRLIADSKGRSAVANARMFALVAMVSDDVGTATGD
                     AKLHYNFWRPITAIRNASDDGNDATLEDPAWEPLIGTPNHPEYPCAHCSGTAAIAAVL
                     KAEVGAAPPGGVRVHSRSIPNSIIQVLPSWDDWVKEVSVSRTYGGVHYRFSNEAGEEM
                     GRKVAEMALARVMRPLPAKRKRKALT"
     gene            complement(1603..2703)
                     /locus_tag="VGB57_11840"
     CDS             complement(1603..2703)
                     /locus_tag="VGB57_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY0132086.1"
                     /translation="MRIGLLTGGGDVPGLNPCIRSITLSADALGWEVTGFRRGWEGVL
                     AIDPADPASVAANSLPLTREGVRGIDRMGGTILHTSRTDPRTLAEGDQTKRVLETLST
                     LGIDAMITLGGDGTLRFSAHLAAQGVPVVSVPKTMDNDVFGTDYCIGFSTAVSRSVEA
                     INALRTTVESHERIGVVELFGRRSGETALLAGFLAQVDRTVIAEVPADLDILMPLLAD
                     DKASNPAGYAMCVISEGASIKGSAEGDSELSKSASERGEEGIGQQLGRAIKARLGFGT
                     VIQELAYLMRAGEPDAMDRMVGFAFGGLAVQLLQRGETARMVTLADGNYGHVPIDTLL
                     SGRRSVDVDGLYDPLTYRARLMRIEGMPMFLY"
     gene            complement(2707..3213)
                     /locus_tag="VGB57_11845"
     CDS             complement(2707..3213)
                     /locus_tag="VGB57_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132087.1"
                     /translation="MLGPAPTAPEPENYDAFAHEPPFRPRRNPAKMWTLLAVLAAFLM
                     LAATAAVYSFGLPQLGVDITLPGRSSTPLEVVQDSIEPRNQTLASGNALLTVTGRIIN
                     PTQKVQRVPQLRAEVRDPSGKVVHSWSISPPVSELQPGQSATFNSAEMDVPTGEGRRL
                     SVKFPSAG"
     gene            complement(3352..3447)
                     /locus_tag="VGB57_11850"
                     /pseudo
     CDS             complement(3352..3447)
                     /locus_tag="VGB57_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
     gene            3669..4430
                     /gene="ftsE"
                     /locus_tag="VGB57_11855"
     CDS             3669..4430
                     /gene="ftsE"
                     /locus_tag="VGB57_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY0132088.1"
                     /translation="MPLRRAGRGPERRRDLNTSGTIVQFDNVGLRYGTGAETLSDLSF
                     SLRHGGFYFLTGPSGAGKTSLLKLLYLAQRPSRGLIKLFGEDIVTMPRKRLPGFRRRI
                     GVVFQDFRLVTHLSAFDNVALPLRVAGVDEKDLQGPVSEMLAWVGLGHRASARPATLS
                     GGEQQRVAIARAVIARPELLVADEPTGNVDPEMAKRLLHLFDSLNKLGTTVVVATHDL
                     HLISSIGRSEMLRLDRGTLLDPTGALRHPPRRAEA"
     gene            4448..5317
                     /locus_tag="VGB57_11860"
     CDS             4448..5317
                     /locus_tag="VGB57_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0132089.1"
                     /translation="MGAADRRLLPEGRLAGPMPWVIAIMMFLTVLAAAAGLGLGGAAA
                     SLGAGLGNRVTVQILEPNPDRREAEAARAAARLQSLPEVESVRRLSAAEMAELLEPWL
                     GGGGLEGDLPIPAMIDAQLIPGARADSLRPAVTAAAPSARVDDNGQWLAPLGRLISAL
                     KWLALGLVALMIGATAATVVLAARAALDTHRGTIEVLHLMGATDVQVARLFQRRIALD
                     ALFGGLVGFGAAALALILIGQRIGALGSDLLGSANLPATSWLALALLPVLGVGLAMLV
                     ARSTILRALGRML"
     gene            5314..5844
                     /locus_tag="VGB57_11865"
     CDS             5314..5844
                     /locus_tag="VGB57_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0132090.1"
                     /translation="MIRRIVSAIVLLYLLGYAVFAVALPRPADERQTDAIVVLTGGAK
                     RIERGLDLMARNKAKRMLVSGVARTVRPEELAAQYRADDSLFACCVDLGRESVDTRSN
                     AEEVARWMDKHKFRSMRLVTTDWHMPRARFELSRRLGGGIAVVGDAVESNPSFAQLFT
                     EYNKYLLRRSAVLVGI"
     gene            5844..6530
                     /locus_tag="VGB57_11870"
     CDS             5844..6530
                     /locus_tag="VGB57_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0132091.1"
                     /translation="MNLVRSLLYALVFYPGSVLYVLSGIAVLPLGKGPVRRVADGWTA
                     FNRFCARFLLGIRTRIEGEVPSGAVIVASKHQSMFETTEIQLILDTPATVMKSELGRI
                     PLWGRLTRAYGIIPVDRDGGGAALRKLLRAAGEAVAEGRPIVIFPEGTRVSPGDRPPL
                     EPGFAGLYRALNLPVVPVALDSGRIWPRRSFVKRPGVVTMRFGEPIPPGLPRKEAEAT
                     VHAAINALEG"
     gene            complement(6623..>7160)
                     /locus_tag="VGB57_11875"
     CDS             complement(6623..>7160)
                     /locus_tag="VGB57_11875"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEY0132092.1"
                     /translation="QLRPEWTGAPPPDAPVEVTRRRGVDLHPVDPRRDGERLLAYVWP
                     DQSRRLAQLEAALAIAGEDPPEVDRGDAADWLEARLADRQASGVTRVVLHSIAFQYFP
                     DSTKARIAGAMEAAGAAATFAAPLAWLRYEHEGGERIGLRLRAWPSGEERLLAHCHPH
                     GREVEWLWCRGSATENGV"
BASE COUNT         1037 a         2528 c         2488 g         1107 t
ORIGIN      
        1 tccacggcct ttcggccaca gcgacctcaa cgctgcgcgt ctaccagttc cgccacgacc
       61 gcacgtcact gccccccgaa aagaagctcc ggggccggta ggagcggggc ctctagcaaa
      121 gggcttaaag ggacgcaatg ccgccctcgc cccgaaaaca gggacagtcc ctattttcgc
      181 ctggcgctga aaaagaggga cagtccctat ttccgcggcg gccggaaata gggactgtcc
      241 ctgtttttag gtcagggcct tgcgtttccg cttggccgga agcggccgca tcaccctggc
      301 gagcgccatt tcggcgacct tgcggcccat ctcctcgccg gcctcgttgg agaagcggta
      361 atggactccg ccataagtgc gcgagacgga gacttccttc acccaatcgt cccagctcgg
      421 aagcacctgg atgatgctgt tggggatcga gcgcgaatgg accctgacgc cgcccggcgg
      481 cgccgctccg acctcggcct tgagcaccgc tgcgatggcg gcggtgccgc tgcaatgggc
      541 gcagggatat tcgggatggt tgggtgtccc gatcaagggc tcccaggccg gatcctcgag
      601 ggtcgcgtcg ttgccgtcgt ccgaggcgtt gcggatggcg gtgatcggcc gccagaaatt
      661 gtagtggagc ttggcgtcgc cggtggcggt tccgacgtcg tccgagacca tcgccacgag
      721 agcgaacatg cgggcgttcg ccaccgccga gcggcctttg gagtcggcga tgaggcgaag
      781 ggtcggagtc atgttgggcg tgatccggta gcgcgccatc agcgactgct ggggagtccg
      841 cgcgctgctc gtcttcgagc cgagccgctt gatctcctcg aagtcgcggg cccagcgctc
      901 gctgttcagc gcgggcgggg gaccaggccg aaccgaatcg taggtcggca agatccacgg
      961 tttgaacgtg aaggcgtagg gctggtagac ggggagctgg gtcgcggtcc acattccggc
     1021 cgaggtgcgc gggcgatagg gcaccggctt gcgcgccggg tcgaatccgc ccgcggcgag
     1081 cgccagcttc gccgccgcct cgcccaccgc cttgccgcgc tcgcgggcgg cggagtcggg
     1141 aatccccagc atggtgacgt cgtagctgtc gtcgaggccg gctttctggc ccggataatg
     1201 ggcgatcagc acctggtagg cggcggtcgc ggcggcggcg tcctgcgagg cgcccggcgc
     1261 ggcggccgcc attccgagat aggattcgaa gcggcggtcg atcgcgttca gggcctcgaa
     1321 catggcgatc gcgacctggg tctgggcgcg gtcgtgctcg ccggtccggg gcgcgtcggc
     1381 cggcggcgcg gcggcggtgc cgaccgtctg ggcatagtcg atccactcgc agaccgtgtc
     1441 cgccgaagcg ggcgaggcgg cgagcagcgc ggcgccggcc gccagaagca gatatctgcg
     1501 catttgaagt ccccccgatt gctccccggc gaaggccggg gcccagcttc accctcggaa
     1561 ggactggacc ccggctttcg ccggggaact tctagcacgc aatcagtaga ggaacatcgg
     1621 catcccttcg atccgcatca aacgcgcacg ataggtcaag ggatcgtaga ggccgtccac
     1681 gtccaccgac ctgcgcccgc tgagcagggt atcgatcggc acatggccgt aattgccgtc
     1741 ggccagggtc accatccggg ccgtctcgcc gcgctggagg agctggacgg cgaggccgcc
     1801 gaaggcgaag ccgaccatcc ggtccatcgc gtcgggctcg ccggcgcgca tcagataggc
     1861 gagctcctgg atgacggtgc cgaagccgag ccgggccttg atcgcccgac cgagctgctg
     1921 gccgatgccc tcctcgcccc gctcgctggc cgatttggaa agctccgaat cgccctcggc
     1981 cgagcccttg atgctcgccc cttccgagat cacgcacatc gcgtagccgg cggggttgga
     2041 ggccttgtcg tcggcgagga ggggcatgag gatgtcgagg tccgccggga cctcggcgat
     2101 gacggtccgg tcgacctggg ccaggaagcc ggcgagcaag gcggtctcgc cgctgcggcg
     2161 gccgaacagt tcgacgaccc cgatccgctc gtggctctcc acggtagtcc tgagcgcgtt
     2221 gatcgcctcg accgagcggc tgaccgcggt cgagaagccg atgcaataat cggtgccgaa
     2281 gacgtcgttg tccatcgtct tggggacgga gacgaccggg accccctgcg ccgcgagatg
     2341 cgccgagaag cgcagtgtgc cgtcgccgcc cagggtgatc atcgcgtcga tgccgagcgt
     2401 cgagagcgtt tcgagaacgc gcttcgtctg gtccccttcc gccagcgtcc gcgggtcggt
     2461 gcgcgaagtg tggaggatcg tcccgcccat ccggtcgatg ccgcggacgc cctcccgggt
     2521 gagcggaagg ctgttggcgg cgacgctggc cgggtcggcc ggatcgatcg caagcacgcc
     2581 ttcccagccc cggcggaagc cggtcacctc ccagccgagc gcgtcggcgg acaaggtgat
     2641 cgaacggatg cagggattga gacccggaac gtcgccgccg ccggtgagca ggccgatgcg
     2701 catgtctcag cctgccgagg gaaacttgac gctcagcctg cggccctccc cagtcggcac
     2761 gtccatctcg gcgctgttga aggtcgcgct ctggcccggc tgcagctcgg agacaggcgg
     2821 cgagatcgac cagctgtgga cgaccttgcc cgaaggatcg cggacttcgg cgcggagttg
     2881 cggcacgcgc tggaccttct gggtcgggtt gatgatccgc ccggtgacgg tgagcaaggc
     2941 gttgccgctc gccagggtct ggttcctggg ctcgatggaa tcctggacga cctccagcgg
     3001 agtcgagctc cggccgggaa gggtgatgtc gacccccagc tggggcagac cgaacgaata
     3061 gacggcggcg gtcgccgcca gcatcagaaa ggctgcgagc acggcgagta gggtccacat
     3121 cttcgccgga ttgcgtctcg gccggaaggg gggctcgtgg gcgaaggcgt cgtaattttc
     3181 cggctccggc gccgtcgggg ccgggcccag catcgaggcc gctggacgcg cgggctccgg
     3241 cttgcgcagg ggcggtgggg gcggcggtgg agccgatgat gccggccgag cctcgggcgc
     3301 ctgccaatcg gcggcggtgc ggggcgacgg cggcggctgc agccagctat gcctgcagga
     3361 agcgcagcgc acctgccgcc cggacgggcc gaccgcgctg tccggcacca cgtagcgcgt
     3421 cttacaggcg ggacagctca ggatcatctt tacgggaggc cccaaaaggc tggtgctcgt
     3481 ccgaatccaa gcacggcggg gcgctccatg caagccattg cgccgtttat accgtcattc
     3541 cggcgaaagc cggaatccat cgacacagcg gcacgaaaac ctctcacggc ggtgttcatg
     3601 gatcccgggt caagcccggg atgacgggcg cgacggccca ttgagtggtt tacggccccg
     3661 cgcggcgcat gccattgagg cgggcggggc gaggccccga gcggaggcgg gatctgaaca
     3721 ctagcggcac catcgtccag ttcgacaatg tcgggctgcg ctatggaacg ggggcggaga
     3781 cgctgtcgga cctgagcttt tcgctcaggc acggcggctt ctacttcctc accgggccgt
     3841 cgggggcggg caagacctcg ctcctgaagc tcctctacct cgcccagcgg ccgagccgcg
     3901 gcctgatcaa attgttcggc gaggacatcg tcacgatgcc gaggaagcgg ctgcccggat
     3961 tccggcggcg gatcggcgtc gtcttccagg atttccgcct cgtcacccat ctctccgcct
     4021 tcgacaatgt cgctctgccc cttcgcgtgg ccggagtgga cgagaaggac ctgcaggggc
     4081 cggtgagcga gatgctggcc tgggtcggcc tcggccaccg cgccagcgcc cgccccgcca
     4141 ctttgtcggg cggcgagcag cagcgcgtcg ccatcgcccg ggccgtcatc gcccggcccg
     4201 aattgctcgt cgccgacgag ccgaccggaa acgtcgaccc ggaaatggcc aagcggctcc
     4261 tccatctgtt cgattcgctg aacaagctcg ggacgacggt ggtcgtcgca acccacgatc
     4321 tccacctcat ctcgagcatc ggccgatcgg agatgcttcg cctcgaccgg ggcacgttgc
     4381 tcgatccgac cggcgccctt cgccacccgc cgcggcgggc cgaggcgtga gcgctcttcc
     4441 gcggacgatg ggcgcggccg accgccggct tctgcccgaa ggccggctgg cggggccgat
     4501 gccctgggtg atcgcgatca tgatgttcct gaccgtgctc gccgccgccg ccggcctcgg
     4561 cctcggcggc gccgcggcga gcctcggcgc cgggctcggc aaccgggtga cggtgcagat
     4621 cctggagccc aacccggacc ggcgcgaggc cgaggcggcg cgcgccgccg cccgcctgca
     4681 aagccttccg gaggtcgaga gcgtccgccg gctttccgcc gccgagatgg cggagctgct
     4741 cgagccctgg ctgggcggcg gcggcctcga gggcgatcta ccgatcccgg cgatgatcga
     4801 cgcccagctc atccccggcg cccgcgccga ctcgcttcga ccggcggtga ccgcggccgc
     4861 cccctcggcc cgggtcgacg ataacggcca gtggctggcg ccgctcggcc ggctgatctc
     4921 ggccctgaaa tggctcgcgc tcggcctcgt cgcactgatg atcggggcca ccgccgccac
     4981 cgtcgtcctc gccgcccggg ccgccctcga cactcaccgc ggcaccatcg aggtgctcca
     5041 tctgatgggg gcgaccgacg tccaggtcgc ccggctgttc cagcgccgga tcgcgctcga
     5101 cgccttgttc ggaggcctgg tcggcttcgg agcggcggcg ctcgccctga tcctgatcgg
     5161 ccagcgcatc ggcgccctcg gctcggatct gctcggctcg gccaatctgc cggcgaccag
     5221 ctggctcgcc ctcgccttgc ttccggtcct cggcgtcggc ctcgccatgc tggtcgctcg
     5281 ctccaccatc ctcagggccc ttgggaggat gttgtgatcc gccgaatcgt ttcagcgatc
     5341 gtccttctct atctgctcgg ctacgcggtc ttcgcggtgg ccctgccgcg gccggcggac
     5401 gagcggcaaa ccgacgccat cgtcgtcctt accggcggcg ccaagaggat cgagcgcggc
     5461 ctcgacctga tggcgcgcaa caaggcgaaa aggatgctcg tctccggcgt cgcccggacc
     5521 gtccgaccgg aggagcttgc ggcgcaatat cgggcggacg attcgctgtt cgcatgctgc
     5581 gtcgacctgg ggcgcgaatc ggtcgacacc cgctccaacg ccgaggaggt cgcgcgctgg
     5641 atggacaagc acaagttcag gtcgatgcgc ctcgtcacca ccgactggca catgccccgc
     5701 gcccgcttcg agctctcccg gcgcctcggc ggcggaatcg ccgtcgtcgg cgacgcggtc
     5761 gagagcaacc cgagcttcgc ccagctcttc accgaatata acaaatatct cctgcgccgc
     5821 tcggccgtgc tcgtcgggat ctgatgaacc tcgtccgctc gctgctctac gcgctcgtct
     5881 tctaccccgg cagcgtcctc tacgtgctct ccgggatcgc cgtgcttccg ctcggcaagg
     5941 gcccggtgcg ccgggtcgcc gacggctgga ccgccttcaa ccgcttctgc gcccgcttcc
     6001 tgctcgggat acgaacccgg atcgagggcg aggtcccgag cggggcggtg atcgtcgcca
     6061 gcaagcacca gtcgatgttc gagacgaccg agatccagct catcctcgac acgccggcga
     6121 cggtgatgaa gagcgagctc ggccggatcc cgctctgggg ccggctgacc cgcgcctacg
     6181 ggatcatccc ggtcgatcgc gacggcggcg gagcggcgct ccgcaagctg ctccgggcgg
     6241 ccggcgaggc ggtcgcggaa ggccggccga tcgtcatctt ccccgaggga acccgggtct
     6301 cgccgggcga tcggccgccg ctcgagcccg gcttcgccgg cctctaccgg gccttgaacc
     6361 tgcccgtcgt ccccgtcgcc ctcgacagcg gccggatctg gccccgacga agcttcgtca
     6421 aaaggccggg cgtggtgacc atgcgcttcg gcgagccgat cccgcccggc ctgccccgca
     6481 aggaagccga ggcgacggtc cacgcggcga tcaacgcttt ggaagggtaa cgtcgccccg
     6541 gcgaaggctg gggccccggc gggtgaagag ctcggcgccc ttcacccctg ggatgccagc
     6601 ctgcgctggc atgacggtgc ggtcagaccc cgttctccgt cgccgacccc cggcaccaca
     6661 gccactccac ctctctcccg tgcggatggc aatgcgccag cagcctctcc tccccgctcg
     6721 gccaggctct cagcctgaga ccgatccgct cgcccccctc atgctcgtag cgcagccatg
     6781 ccaaaggcgc cgcgaaggtc gccgccgccc ccgccgcctc catcgccccc gcgatccgcg
     6841 ccttggtcga gtccggaaaa tattgaaagg cgatcgagtg caggaccacc cgggtcacgc
     6901 cggacgcctg ccgatccgcg agccgcgcct ccagccagtc cgccgcgtcg ccccggtcca
     6961 cctccggcgg atcctctccg gcgatggcca gcgccgcttc gagctgggcg agccgccgcg
     7021 actggtcggg ccagacgtaa gcgagcagcc gttcgccgtc gcgcctcggg tcgacggggt
     7081 ggagatcgac gccccgccgc cgggtcacct cgaccggcgc gtccggcggc ggagcgcccg
     7141 tccactccgg ccgtagctga
//
LOCUS       DASWNU010000596         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_232255, whole genome shotgun sequence.
ACCESSION   DASWNU010000596 DASWNU010000000
VERSION     DASWNU010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232255"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGB57_11880"
     CDS             <1..232
                     /locus_tag="VGB57_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132093.1"
                     /translation="GCFIGFCAPQGGGGQSAYASLVDLRTGEVAWFNVLQTQSMMPGV
                     GFGDIRTPEGAAQMVERLLGRMKAGRHVRARR"
     gene            229..1446
                     /locus_tag="VGB57_11885"
     CDS             229..1446
                     /locus_tag="VGB57_11885"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0132094.1"
                     /translation="MSVALTRRSLLGAFGCACCLGTVGAAEARIPPRSMKPLIGPGHR
                     PTAKDELGMWQQFERVEQEIAGSNLLLKDPNLASYLRGLAERVGGPAAKDLRVYIAHI
                     PEFNAFMAPTGFMVVFTGLLLRMRDEAQLSGVIAHEAGHFLRRHQIRLWRDMKRKTAA
                     LNFLAMGAGVGGAAGGFYAGDLVQLAQFGTILSLLSYSRELEAEADAIGLLQISEAGY
                     DPSAMPETWQQLIEESRASAAMRRKRYRRRYSLLETHPAPETRMVDLRASAREVTTAR
                     TTERGRERYLRALGEHRKTLLDDQVKLNDPGASLYVIRNLAKDGWSGLLRYYEGEVWR
                     LKGGKGDSELAGRSYAAAIAYPDAPPEAWRAHGYQLLKQGRRDEGKAALNRYLALAPR
                     APDAAMVRHSASQ"
     gene            1474..2100
                     /locus_tag="VGB57_11890"
     CDS             1474..2100
                     /locus_tag="VGB57_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132095.1"
                     /translation="MRRGKMLAALAALSLTAGCAGGGLGGPGLFRYYSLVTPGPEDVA
                     RGSMIVTPTMRWNKAPRGPHDIYREENWTLNGPLLDGLTFIGGLEHDKRIVQQRRKAD
                     RKVPNFRADMNPTEIASMIESFYRIRAGSTRFEMTGLQPREFLGRPGFQFDFAHLGGD
                     EVERKGRAVGAIIDNRFYLALFDATRMHYFDAGLPEFERIVQSARLRG"
     gene            complement(2190..>2781)
                     /locus_tag="VGB57_11895"
     CDS             complement(2190..>2781)
                     /locus_tag="VGB57_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0132096.1"
                     /translation="HRYDEETPLEETMEALTAAVRSGKVRWLGFSEWPADMIRAAVEM
                     AGVEKFVSSQPQYSLLWRQPEAEVIPLCADNGISQIVWSPLAQGVLTGKYRPGSPPPQ
                     GSRATSEEMGGFISWLLRDETLEAVQRLQPIAAEAGLSLAQFALAWVLREPNVASAIV
                     GASRPEQLAENAAASGVEVDPALFAQAEAIAAEVRR"
BASE COUNT          440 a          984 c          942 g          415 t
ORIGIN      
        1 cggctgcttc atcggcttct gcgcgccgca gggcggcggc ggccagtccg cttacgcttc
       61 gctcgtggac ctgaggaccg gcgaggtcgc ctggttcaac gtcctccaga cccagagcat
      121 gatgccggga gtcggtttcg gcgacatcag gacgcccgag ggcgcggcgc agatggtcga
      181 gcgcctgctc ggccggatga aggccgggcg ccacgtcagg gcgaggcggt gagcgtcgct
      241 ctcacccggc gctccctcct cggcgccttc ggctgcgcct gctgcctggg gacggtcggc
      301 gccgcggagg cgcggatccc gccgcgctcg atgaagcccc tgatcggccc cggccaccgc
      361 cccaccgcca aggacgagct cggcatgtgg cagcagttcg agcgggtcga gcaggagatc
      421 gccggctcca acctgctcct caaggacccg aacctcgctt cctatctgcg cggccttgcc
      481 gagcgggtag gggggccggc tgcgaaggac cttcgagtct acatcgccca cattcccgag
      541 ttcaacgcct tcatggcgcc gaccggattc atggtcgtgt tcaccggcct gctcctgcgg
      601 atgcgcgacg aagcccagct ctccggagtg atagcgcatg aggccggcca tttccttcgc
      661 cgccaccaga taaggctgtg gcgcgacatg aagcgcaaga ccgcggcgct caacttcctc
      721 gccatggggg cgggcgtcgg cggagcggcg ggcggcttct acgccggcga cctcgtccag
      781 ctcgcccagt tcgggaccat cctctcgctc ctgtcctaca gccgcgagct cgaggcggaa
      841 gcggacgcga tcgggctcct gcagatcagc gaggccggct acgatccctc tgcaatgccg
      901 gagacctggc agcaattgat cgaggagagc cgggcgagcg cggcgatgcg ccgcaagcgc
      961 taccgccgcc gctattcgct gctcgagacc catccggcgc cggagacccg gatggtcgat
     1021 cttcgagcgt cggcgaggga ggtgacgacg gcgcgcacca ccgagcgcgg gcgcgagcgt
     1081 tatctccggg cgctgggcga gcaccgcaag accctgctcg acgatcaggt caagctcaac
     1141 gatcccgggg ccagcctcta cgtcatccgc aacctcgcca aagacggctg gagcggcctg
     1201 cttcgttatt atgaaggcga ggtctggcgc ctcaagggcg gcaagggcga cagcgagctc
     1261 gccggacgga gctatgccgc agcgatcgcc tatcccgacg ccccgccgga agcctggcgg
     1321 gcgcacggct accaattgct caagcagggc cggcgcgacg agggcaaagc ggcccttaac
     1381 cgctacctgg cccttgcccc gcgagcgccc gatgcggcca tggtccgcca ctcggccagt
     1441 caatgagagg ggccagtgag aggagaaggg ccgatgcgta gagggaagat gctcgcggcg
     1501 ctggcggctt tgtcgctgac cgcgggctgt gccggcggcg gcctcggcgg cccgggcctg
     1561 ttccgctatt attcgctggt cacaccgggc cccgaggacg tcgcccgcgg ctcgatgatc
     1621 gtcacgccca cgatgcgctg gaacaaggcg ccgcgcggcc cgcacgacat ctaccgcgag
     1681 gagaattgga ccctgaacgg gcctctgctc gacgggctca ccttcatcgg cggactcgag
     1741 cacgacaaga ggatcgtcca gcaaaggcgc aaggcggacc gcaaggtgcc caatttccgg
     1801 gccgacatga acccgaccga gatcgcctcg atgatcgaga gcttctaccg gatccgcgcc
     1861 ggctcgaccc ggttcgagat gaccggcctc cagccccgcg aattcctggg tcgtcccggc
     1921 ttccagttcg atttcgccca tctcggcggc gacgaggtcg agcggaaggg cagggcggtg
     1981 ggagcgatca tcgacaaccg cttttacctg gccctgttcg acgcgacccg gatgcactat
     2041 ttcgacgcgg gtttgccgga gttcgagcgc atcgtgcaga gcgcgcggct caggggctaa
     2101 ccccccgtca tcccggcgaa agccgggacc tcagggagac ccaccgggac atctcggcct
     2161 gaggtcccgg cttgcgccgg gatgacgact tacctccgca cctccgcggc gatcgcctcg
     2221 gcctgagcga acagcgccgg atccacctcc acccccgacg ccgccgcatt ctccgccagt
     2281 tgctcgggcc tcgaagcgcc gacgatggcg gaagcgacgt tgggctcccg aagcacccag
     2341 gccagggcga attgggcgag gctgaggccg gcctcggcgg cgatcggctg gagccgctgc
     2401 accgcctcca gcgtctcgtc cctgagcagc caggagatga agccgcccat ctcctcgctg
     2461 gtcgcccggc tgccctgcgg cggcgggctg cccggccggt acttgccggt gagcacgccc
     2521 tgcgccagcg gcgaccagac gatctgcgag attccgttgt cggcgcaaag cgggatcacc
     2581 tccgcctcgg gctgccgcca gagcagcgaa tattgcggct ggctggagac gaacttctcc
     2641 actccggcca tctccacggc ggcccggatc atgtccgcgg gccattccga gaagccgagc
     2701 cagcgtacct tgccggagcg caccgccgcg gtcagtgcct ccatcgtctc ctcgagcggg
     2761 gtctcctcat cgtagcggtg g
//
LOCUS       DASWNU010000597         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_385553, whole genome shotgun sequence.
ACCESSION   DASWNU010000597 DASWNU010000000
VERSION     DASWNU010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385553"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..511
                     /locus_tag="VGB57_11900"
     CDS             53..511
                     /locus_tag="VGB57_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0132097.1"
                     /translation="MILPITLTIAGAAALLNIWLARRVGQMRLAHKVSIGDGGNEALI
                     ARMRAQANFIEYTPFVLILIGLIELAEGSKLWLWIVGVVYVLGRIAHGFGMDRPRGDP
                     LKLRTVGIVVTALILFGLALYAIVLPYIHREPARVTYAEASSVVPGAKLS"
     gene            complement(466..1392)
                     /locus_tag="VGB57_11905"
     CDS             complement(466..1392)
                     /locus_tag="VGB57_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0132098.1"
                     /translation="MRTTIPAEIEADIKRAVRLECWTIAWMASIIVLIGLVMGSSQAM
                     KTAWIEDILSLVPSLVFLLALHFERKPPTDRFPYGYHRVNSLAFLVAAVALSVMGAVL
                     LVESAVSLIHAEHVTIPPVLLFGEEIWLGWLMVAALAYSIVPPVILGRMKLPLARRLQ
                     DEVLHTDALMQKADWMTGLAGIAGIVGIGLGYWWADAVAAGIISFSILHDGITALRIA
                     VAELADGAPRELGGTRLEEEAGALRARLLELYPGADVRLRDSGRFILAQVCGVAPPGP
                     VDLDSLWPGKPERSWRFAQLSFAPGTTDEASA"
     gene            complement(1389..1820)
                     /locus_tag="VGB57_11910"
     CDS             complement(1389..1820)
                     /locus_tag="VGB57_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132099.1"
                     /translation="MIRTFFLATTLLAASCDSIPADPGGTLERVRGEGLFRVGLIASH
                     APPPEEAADLIQRLSRATGAKAALERGAAEALLTRLEEGDLDLVLGEFAEKSPWAARV
                     TLSEPIVATGPHRLAAAARNGENAWIALVHREARAAASAAR"
     gene            complement(1902..2561)
                     /locus_tag="VGB57_11915"
     CDS             complement(1902..2561)
                     /locus_tag="VGB57_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0132100.1"
                     /translation="MTEPPMKAVIVPVTPLQQNCCLLWCTKTMLGTFTDPGGDLDRLK
                     AAAKQHNVTIEKILLTHGHIDHCGSAKILADELGVKIEGPHIADKFWIDRLAEDGARY
                     GIMGQPFESDRWLGDGDTATVGELVFDVYHCPGHTPGHIVFHHAPSKLAIVGDVLFQG
                     SIGRTDFPMGNHQDLLDAITTKLWPLGGDTAFVPGHGPMSTFARERATNPFVGDRVLA
                     G"
     gene            2724..>3003
                     /locus_tag="VGB57_11920"
     CDS             2724..>3003
                     /locus_tag="VGB57_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY0132101.1"
                     /translation="MTLTHLQRLEAEAIHVMREAAAEFERPVMLYSAGKDSSVLLHLA
                     VLAFAPARPPFPLLHVDTMWKFKAMYELRDRAAAQAGMELIVHRNPEAE"
BASE COUNT          479 a          996 c         1058 g          470 t
ORIGIN      
        1 gcaagcgcgg ccgccgcgtg cgacttcgcg gccaggatag ggggagaagg ccatgattct
       61 gccgatcacg ctgaccatcg ccggagccgc ggccctgctc aacatctggc tggcgcggcg
      121 cgtgggccag atgcgcctcg cgcacaaggt ctcgatcggg gacggcggca acgaggcgct
      181 gatcgcccgg atgcgcgccc aggccaattt catcgaatat actccgttcg tgctcatcct
      241 catcggcctc atcgagctgg ccgagggctc gaagctgtgg ctgtggatcg tcggagtcgt
      301 ctacgtcctc ggccggatcg cccacggctt cgggatggac aggccccgcg gcgacccgct
      361 gaagcttcgg acggtcggga tcgtcgtgac cgccttgatc ctgttcggcc tcgcgctcta
      421 cgcgatcgtc cttccctaca tccatcgcga gccggcgcgg gtcacctatg cggaagcttc
      481 gtcggtcgtc cccggcgcga agctgagctg agcgaagcgc cagcttcgtt ccggcttgcc
      541 gggccacagg gagtcgaggt cgaccgggcc gggcggggcg actccgcaga cctgggcgag
      601 gatgaagcgg cccgaatcgc ggagccgcac gtccgcgccc ggatagagct ccagaagcct
      661 cgccctgagc gccccggcct cttcttccag ccgggtgccg ccaagctcgc gcggagcgcc
      721 gtcggcaagc tcggcgaccg cgatccgaag cgcggtgatc ccgtcgtgga ggatgctgaa
      781 cgagatgatt cccgcggcca ccgcgtcggc ccaccaatag ccgagcccga ttccgacgat
      841 cccggcgatg ccggcgaggc cggtcatcca gtcggccttc tgcatgagcg cgtcggtgtg
      901 gagcacctcg tcctgcaggc gccgggccag cggcagcttc atccggccga ggatcaccgg
      961 cgggacgatc gaataagcga gcgccgcgac catcagccag ccgagccaga tctcctcccc
     1021 gaagagcaac accggcggga tcgtcacatg ctcggcgtgg atgagggaaa cggccgattc
     1081 gaccagaagc accgcgccca tcaccgagag agcgaccgcc gcgaccagga aggcgaggct
     1141 gttcacccgg tgatagccgt aagggaagcg gtcggtcggc ggcttgcgct cgaaatggag
     1201 ggcgaggagg aagacgagcg agggcacgag gctgaggatg tcctcgatcc aggccgtctt
     1261 catcgcctgg ctcgatccca tgaccaggcc aatgagcacg atgatcgaag ccatccaggc
     1321 gatcgtccaa cattcaaggc ggacggcccg cttgatatcg gcttcgatct cggcggggat
     1381 ggtcgtcctc atcgcgcggc cgatgcggcg gcccgcgctt cgcgatggac gagggcgatc
     1441 caggcattct cgccattcct ggcggcggcc gcgagcctat ggggaccggt ggcgacgatc
     1501 ggctcgctca gggtcacccg cgccgcccag gggctcttct cggcgaactc gcccagcacc
     1561 agatcgagat cgccttcctc cagccgggtc agaagcgcct cggccgcccc ccgctcgagc
     1621 gccgccttcg cgccggtggc tcgcgacaga cgctggatca ggtcggcggc ttcctccggc
     1681 ggcggcgcat gggaggcgat aagtccgacc ctgaacaggc cttcgccccg gactcgctcg
     1741 agagtcccgc cggggtcggc cgggatggag tcgcagctgg cggccagcag cgtcgtcgcc
     1801 aaaaagaagg tccggatcat tacggcggca gaacgccgcc cggggacggc tcgttcctta
     1861 catggatcaa ctcgtcattc cggcttccgc cggaacgacg gtcagcccgc cagcaccctg
     1921 tcgccgacga aggggttggt ggcgcgctcg cgggcgaagg tgctcatcgg cccgtggccg
     1981 gggacgaagg cggtgtcgcc gccgagcggc cacagcttgg tggtgatggc gtcgagcagg
     2041 tcctggtggt tgcccatcgg gaaatcggtt cggccgatcg agccctggaa caggacgtcg
     2101 ccgacgatgg cgagcttcga gggggcgtgg tggaagacga tgtggccggg cgtgtggccg
     2161 gggcaatgat agacgtcgaa gaccagctcg cccacggtcg cggtgtcgcc gtcgcccagc
     2221 cagcggtccg attcgaacgg ctggcccatg atgccgtagc gggcgccgtc ctcggcgagc
     2281 cggtcgatcc agaacttgtc ggcgatgtgc ggcccttcga tcttcactcc gagctcgtcg
     2341 gcgaggatct tggccgatcc gcaatggtcg atatggccgt gggtgaggag gatcttctcg
     2401 atcgtgacgt tgtgctgctt cgccgccgcc ttcaaccggt cgaggtcgcc gccggggtcg
     2461 gtgaaggtgc cgagcatcgt cttcgtgcac cagagcaggc agcaattctg ctggagcggg
     2521 gtcaccggga cgatcacggc tttcatcgga ggttcggtca tcggggcgag atggcgcggg
     2581 cgaggcgaag ttgcaaccgc gcgccctctc cgtcgtccca gcgaaagcgg ggacctcggg
     2641 acaggaagtc tcggcgggtc tccccgaggt cccggctttc gccgggatga cgggagcgat
     2701 tccgatccgc taagggggtg acgatgacgc tcacccacct ccagcggctc gaggccgagg
     2761 cgatccacgt catgcgcgag gccgccgccg agttcgagcg gccggtaatg ctctattcgg
     2821 ccggcaagga tagctcggtg ctgctccatc tggccgtgct ggccttcgct ccggccaggc
     2881 cgcccttccc gctcctccac gtcgacacga tgtggaagtt caaggcgatg tacgagcttc
     2941 gcgaccgggc cgcggcgcaa gcggggatgg agctgatcgt ccaccgcaac ccggaggccg
     3001 agg
//
LOCUS       DASWNU010000598         6368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_308051, whole genome shotgun sequence.
ACCESSION   DASWNU010000598 DASWNU010000000
VERSION     DASWNU010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308051"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGB57_11925"
     CDS             <1..318
                     /locus_tag="VGB57_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY0132102.1"
                     /translation="QSPTQPRRGAPHTMDIALAFGTLDAEGSYTGTGAEARALSQAMM
                     KAFTAFARRGNPNGTGVPAWPAYRLPGRTTMVWDRKSAAVADPRGWERELFARVPYVQ
                     PGT"
     gene            592..3492
                     /locus_tag="VGB57_11930"
     CDS             592..3492
                     /locus_tag="VGB57_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0132103.1"
                     /translation="MRHISGAVSATVLALALAAPAHAQDATNLPPTSPQGADGDGVPN
                     ADDAAANNEAAPVEAAAEEQTSEDIVVTGFRASLRRSLSEKREATGIIDVIKAEDIAD
                     FPDNNLAESIQRIPGVAITRDQGEGRNITVRGLGPIFTRVRINGMEGLSTTGGADSSG
                     GANRGRAFDFNVFASELFNSITVRKTASADVEEGSLGATVDLVTARPFDYRDDFTLAG
                     SAQLGYNDLSTAIDPRLAALVSAKFADGKVGILLSAAYSERGIREEGPSTVRWERGTD
                     NGGFAPASTLPAGASQGFAFFHPRIPRYDSYRYSTERLGLTGSLQFQPADSTLLTFDA
                     LYADFKSNRSEQYLEAISFSRSGTGKPQTVILPGAQIDSTNSLVSGTFNNVDVRVESR
                     FDELQTRFQQYTGSLHQDFGERLRLDLLAGYSRSDFSNPVQTTVALDALNVQGYSYDF
                     TNGRNPTFNYGSLDVTNPASFILGEIRLRPQYVDNTFKIARAQVEFDVTEALTVRAGG
                     DYKKYGYESQEFRRASETDVAGLVPVGQLGPLSTLYSFSSKTDLNGTPPTFVVPNLQG
                     FADTLGIYSNTGRFELTGITNASARGNFTTVDERDLGAFAQAVLDTEVGGMRLRADAG
                     LRYVETKQTSTGYQNGGPPPGYTLIEVTRTYDNWLPSANVSLNVTSDFIVRAAAAKVM
                     ARPNIATVNPGGSFSISGGNRTLTLGNPFINPTEATDYDLSLEWYFARESALVVGLFY
                     KDISSFVATTTQQIPFNQLGLPESLLTGTNVQPTDLFTVTQPVDSEGGSLKGFEIGVQ
                     APFRFLPGLLSNVGIQANYTFVKSDITYPLATAANAPSVTRPLVNLSKHSANATLYYE
                     DDTFSLRGSVSYRSGYLTAVPGRNGVAPGAGPVFNPNSGQPTFNDVEGTHGSINIDAA
                     ASVKINDNFSLTFEAINLTDEYTDQYIDSAADRLSVYHHTGRQFYVGGRFKF"
     gene            3653..5263
                     /locus_tag="VGB57_11935"
     CDS             3653..5263
                     /locus_tag="VGB57_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0132104.1"
                     /translation="MHIDRRTALVGAVAGGAVALLPRPLWAQGSHASLDPHSEARWGK
                     GFEGQRKADLGNGHFLNPIVAGDHPDPTILKDGADYYMTFSNFDSYPGLVIWHSRDLV
                     NWRPVTAALSRNIGSVWAPELIKHEGRYFLYIPVKASLNTSYVIWADRIEGPWSDPID
                     LKLPNHIDPGHAVGEDGSRWLFLSGGDRVRLSDDGLRRIGEPEHVYDPWRYPSDWIVE
                     GFSPEGPKITRHGDYFYLVTAVGGTAGPPTGHMVIAARSRSIHGPWENHPDNPLVRTT
                     DVRERWWSRGHATLVEGPAGDWWTVYHGYENGFWTLGRQTLLDRIEWTADGWFRMTGG
                     DLSKPLPKPKGGRAVPHGQPLSDGFATLALGSKWNFFKPAPDERSRARVEKNRLVLKA
                     RGKAPSDSPPLLLIAGDQAYRFEADVEIDPGGVAGLILFYDERLYAGLGFDQTRFVTH
                     QYGIERGRPANPHGRRMRMRVTNDRHIVTYDTSGDGGRSWRRFDRGMEVSGYHHNVRG
                     GFLMLRPGLYSAGQGEARFSNFTYRALA"
     gene            5260..6258
                     /locus_tag="VGB57_11940"
     CDS             5260..6258
                     /locus_tag="VGB57_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0132105.1"
                     /translation="MKIDRRTALLGSLALGLAAGARAQTRPPATPDSPHMAAPDPTET
                     IDLWPRGAPGAPPTLPLETVRERSKDPAYNDRYVFGISRPRMAVFRPARPNGAAVLIT
                     PGGGYNWVVIDKEGYEMARLLAARGITAFVLFYRLPHEGWAAGPDTPLSDAQRAMRLI
                     RHRAARYGIDRKRVCAMGFSAGGHLCADLMTRFDTRVYAPVDPADRLSARPDFAAPIY
                     PVVSMALPTAHAGSRRNLVGENASPERERAYSPHLNVPADAPPAFLLHAEDDASVAVE
                     NTLLLRAALLAKKVPVETHLFADGGHGFGLRLARGKTVEGWQELFFAWARKKGLFE"
BASE COUNT         1050 a         2351 c         1942 g         1025 t
ORIGIN      
        1 cagtcgccga cccagccgcg ccggggcgcg ccccacacga tggacatcgc gctggcgttc
       61 ggaacgctcg acgcggaggg gtcctacacc ggcaccggcg ccgaggcgcg ggcgctcagc
      121 caggcgatga tgaaggcctt cacggctttc gcaaggcgcg ggaacccgaa tggcacgggc
      181 gttcccgcct ggccggccta tcggcttccc ggacgcacga ccatggtctg ggacaggaaa
      241 agcgccgccg tcgccgatcc gcgcgggtgg gagcgcgagc ttttcgcccg ggtgccgtac
      301 gtccagcccg gaacatgatc cgggtccggg gttgacaggc ccccgtcgtc ggggcagacc
      361 gatgacaccg gttacttaag atccggtagg ggcgacttcg caaccatagc ggagcggtcc
      421 tgagagagga taaagacgcg cgccgcgcgc tccccacctc cgaacgcggc tcgcgccgcc
      481 accttcgcgc cggctttaga caccggtgtc acaacgggac ggcgggcggc ccgtcgaaca
      541 gggaggttca tcgtcatggt ttcggatcgg cttttcggac gggctcatgc cttgcggcac
      601 atatcggggg ccgtttccgc gaccgtgctc gccctcgccc tcgccgcccc cgctcacgcc
      661 caggacgcaa ccaacctacc cccgacgtcg ccgcagggcg ccgacggcga cggcgttccc
      721 aatgccgacg acgcagccgc caacaacgag gccgccccag tcgaggcggc ggccgaagag
      781 cagacttcgg aggacatcgt cgtcaccggc ttccgcgcct cgcttcgccg ctcgctcagc
      841 gagaagcggg aggcgaccgg catcatcgac gtcatcaagg ccgaggatat tgccgacttt
      901 ccggacaaca acctcgcgga atcgatccag cgcatccccg gcgtcgccat cacccgcgac
      961 cagggcgagg ggcgcaacat caccgtgcgc ggcctcggcc cgatcttcac ccgcgtccgg
     1021 atcaacggga tggagggcct ctccaccacc ggcggcgccg attccagcgg cggcgccaat
     1081 cgcggccggg ccttcgactt caacgtcttc gcctccgagc tgttcaactc gatcaccgtc
     1141 cgcaagaccg cctcggccga cgtcgaggaa ggctcgctgg gcgccaccgt cgatctcgtc
     1201 acggcgcggc cgttcgacta tcgcgacgac ttcaccttgg ccggctcggc gcagctcggc
     1261 tataacgacc tttccaccgc catcgacccg cggctcgccg cgctcgtctc cgcaaagttc
     1321 gccgacggca aggtcgggat cctgctctcc gccgcctata gcgagcgcgg catccgcgag
     1381 gagggcccct cgacggtgcg ctgggagcgg ggcaccgaca acggcggctt cgctccggcc
     1441 tccactttgc cggccggcgc cagccagggc ttcgccttct tccacccgcg gatcccgcgc
     1501 tacgacagct atcgctactc gacggagcgg ctcggcctga ccggctcgct ccagttccag
     1561 ccggccgaca gcaccctgct gaccttcgac gcgctctacg cggacttcaa gtcgaaccgg
     1621 tccgagcaat atctggaggc gatctccttc agccgctccg gcaccggcaa gccgcagacc
     1681 gtgatcctgc ccggcgccca gatcgattcg accaacagcc tcgtttccgg cacgttcaac
     1741 aatgtcgacg tccgcgtcga atcgcgcttc gacgagctcc agacccggtt ccagcaatat
     1801 accggatccc tccatcagga cttcggcgag cgcctccgcc tggacctgct cgccggctat
     1861 tcgcggtccg acttctcgaa cccggtccag accaccgtgg ccctcgatgc gctgaacgtg
     1921 cagggctaca gctacgattt caccaacggc cgcaacccga ccttcaacta cggcagcctc
     1981 gacgtcacca acccggcgag cttcatcctc ggcgaaatcc ggcttcgtcc ccaatatgtc
     2041 gacaatacgt tcaagatcgc acgggcccag gtcgagttcg acgtcaccga ggcgctcacc
     2101 gtgcgcgccg gcggcgacta caagaaatat ggctacgaat cgcaggagtt ccgccgtgcc
     2161 agcgaaacgg acgtcgccgg gttagtgcct gtcgggcagc tcgggccgct ctccacgctc
     2221 tattcgttca gctcgaagac cgatctcaac ggcaccccgc cgaccttcgt ggtgccgaac
     2281 ctccagggct tcgcggacac gctcggaatc tattcgaaca ccggccgctt cgagctgacc
     2341 ggaatcacca atgccagcgc gcgcggtaat ttcacgaccg tcgatgagcg ggacctcggc
     2401 gccttcgccc aggcggtgct cgacacggaa gtcggcggga tgcggctccg ggccgatgcc
     2461 ggcctgcgct acgtcgagac gaagcagact tcgaccggct accagaatgg aggcccgccc
     2521 ccgggctaca ctctgatcga ggtgacccgg acctatgaca attggctccc gtcggccaat
     2581 gtcagcctca acgtcacctc cgacttcatc gtccgcgccg cggcggcgaa ggtcatggcc
     2641 cggcccaata tcgccaccgt caatcccggc ggctccttct cgatcagcgg cggcaatcgc
     2701 accctgacgc tgggcaatcc cttcatcaac ccgaccgagg cgaccgacta cgacctgtcc
     2761 ctggaatggt atttcgctcg cgagtcggcg ctcgtcgtcg ggctgttcta caaggacatt
     2821 tcgagcttcg tcgcgacgac cacccagcag atcccgttca accagctcgg actgcccgaa
     2881 tcgctgctca ccggcaccaa tgtgcagccc accgacctct tcacggtgac ccagccggtc
     2941 gacagcgaag gcggctcgct caagggcttc gagatcgggg tccaggcccc cttccgcttc
     3001 cttccgggcc ttctttcgaa cgtcggaatc caggccaatt acacgttcgt caaatcggac
     3061 atcacctatc cgctcgccac cgccgccaac gctccatcgg tgacccggcc gctggtcaat
     3121 ttgtcgaagc acagcgccaa cgcgaccctc tattacgagg acgacacctt ctcgctgcgc
     3181 ggctcggtct cctaccgttc gggctatctg accgcggttc cggggcgcaa cggcgtggcg
     3241 cccggcgccg ggccggtatt caacccgaat tccggtcagc cgaccttcaa cgacgtcgaa
     3301 ggcacgcacg gctcgatcaa catcgacgcc gccgcctcgg tcaagatcaa cgacaatttc
     3361 tcgctgacct tcgaggcgat caatctcacc gacgaatata ccgaccagta tatcgacagc
     3421 gccgccgacc ggctgagcgt ctatcaccat accggccggc aattctacgt gggcggccgg
     3481 ttcaaattct agtttgtccg gctggtcacg ctccctcaat tcctcccccg gttttccggg
     3541 ggagggggac caggcgaagc ctggtggagg ggccttgctg actccgaaga acccctccac
     3601 cgcgcttcgc gcggtccccc tccccaagaa atctcgggga ggaatcgagg gcctgcacat
     3661 cgatcgccgc actgcgctgg tcggagcggt tgccggcggc gcggtcgcgc tcctgccccg
     3721 accgctgtgg gcgcagggtt cgcacgcctc cctcgatcct cattcggagg cgcgctgggg
     3781 caaaggcttc gaaggccagc gcaaggcgga cctcggcaac ggccacttcc tcaacccgat
     3841 cgtcgccggc gaccatccgg atccaacgat cctgaaggat ggcgccgatt attacatgac
     3901 cttctcgaac ttcgattcct atcccggcct cgtcatctgg cacagccggg acctggtgaa
     3961 ctggcggccg gtcaccgccg ccctctcccg caacatcggc tcggtctggg cgccggagct
     4021 catcaagcac gagggacgct atttcctcta cattccggtg aaggcgtcgc tcaataccag
     4081 ctacgtcatc tgggccgacc ggatcgaggg cccctggagc gaccccatcg acctgaagct
     4141 gcccaaccat atcgaccccg gccacgccgt gggcgaggac ggctcgcgct ggctgttcct
     4201 gtccggcggc gaccgggtcc ggctctccga cgacgggctg aggcggatcg gcgagcccga
     4261 gcacgtctac gatccgtggc gctatccctc cgactggatc gttgaaggtt tctcacccga
     4321 gggccccaag atcacccgcc atggcgacta tttctacctg gtcacggccg tcggcggcac
     4381 cgccgggccg cccaccggcc acatggtgat cgccgcgcgc tcgcgctcga tccacgggcc
     4441 gtgggagaac catccggaca acccgctggt acgcacgacc gacgtccgcg aacgctggtg
     4501 gtcgcgcggc catgccactt tggtcgaggg cccggccggc gactggtgga ccgtctatca
     4561 cggctatgag aacggcttct ggaccctcgg ccgccagacc ctgctcgatc ggatcgagtg
     4621 gaccgcggac ggctggttca ggatgaccgg cggagacctg tcgaagccgc tgcccaagcc
     4681 gaagggcggc cgagcggtcc cgcatggtca gcccctgtcg gacggtttcg ccacgctcgc
     4741 gctcggcagc aagtggaact tcttcaagcc cgcacccgac gaacgctcgc gggcccgggt
     4801 ggagaagaac cggctggtgc tgaaggcccg cggcaaggct ccgtccgatt ccccgcccct
     4861 gctcctcatc gccggcgacc aggcctaccg attcgaggcg gacgtggaga tcgatccggg
     4921 cggagtcgcc ggcctcatcc tcttctacga cgagcgcctg tatgccgggc tcggcttcga
     4981 ccagacccgc ttcgtcaccc atcagtacgg catcgagcgc ggccgccccg ccaatccgca
     5041 cggtcgacgg atgcggatga gggtgaccaa cgaccgccac atcgtcacct acgacaccag
     5101 cggcgacggc ggccgcagct ggcgccgctt cgaccgcggc atggaggtct cgggctacca
     5161 ccacaatgtc cgcggcggct tcctgatgct gcgtcccggc ctttattccg ccgggcaggg
     5221 ggaggcgcgc ttctccaact tcacttatcg ggccctcgca tgaaaatcga ccgccgcacc
     5281 gcattgctgg gaagcctcgc cctggggttg gcggcgggcg cgagggcgca gacccggccg
     5341 cccgcgactc cggattcacc gcacatggcc gctccggatc cgacggagac gatcgacctc
     5401 tggccgcgcg gggcgcctgg cgcgccaccg acgctgccgc tcgagacggt cagggagcga
     5461 agcaaggacc cggcctataa cgaccgctac gtcttcggga tcagccgccc gcgcatggcc
     5521 gtcttccgcc ccgcacgccc gaacggcgcc gcggtcctga tcactccggg cgggggctac
     5581 aattgggtgg tgatcgacaa ggaaggctat gagatggccc ggcttctcgc cgcccgcgga
     5641 atcaccgcct tcgttctctt ctaccgcctg ccccatgagg gctgggccgc cggccccgac
     5701 acgccgctgt cggacgccca gcgcgcgatg cgcctcatcc gccaccgcgc cgcccgctac
     5761 ggcatcgacc ggaagcgggt ctgcgcgatg ggcttctcgg ccggcggcca cctctgcgcc
     5821 gatctgatga cccgcttcga taccagggtg tacgcgccgg tcgacccggc cgatcgcctc
     5881 tccgcccgcc ccgatttcgc cgcgcccatc tatccggtgg tgtcgatggc ccttccgacc
     5941 gcccatgccg gctcgcgccg caacctggtc ggcgagaacg cctcgcccga acgcgagcgc
     6001 gcttattcgc cgcacctcaa tgtgccggcc gacgctccgc ccgcgttcct ccttcatgcc
     6061 gaggacgatg catccgtggc ggtcgagaac acgttgcttc tgcgcgcggc gctgctggcg
     6121 aaaaaggtgc ccgtcgagac ccatttgttc gccgacggcg gccacggctt cggccttcgt
     6181 ctcgccaggg gaaagacggt agagggctgg caggagctgt tcttcgcctg ggcccgaaag
     6241 aagggattgt tcgagtgatc acgagactgt tcgccgcttt gctcctcctg gtcggcctcg
     6301 ccgccccttc caacgcgcag aaccggaccc agatcgagcg gacgatgaag cgcgccacga
     6361 ccttcatg
//
LOCUS       DASWNU010000599         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_127119, whole genome shotgun sequence.
ACCESSION   DASWNU010000599 DASWNU010000000
VERSION     DASWNU010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127119"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /gene="ligA"
                     /locus_tag="VGB57_11945"
     CDS             <1..2025
                     /gene="ligA"
                     /locus_tag="VGB57_11945"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0132106.1"
                     /translation="PEISDAEYDALVRRNAELEAAFPHLARPDSPSRAVGAAPAAHLS
                     KVRHALPMLSLENAFSDEEVADFMARVRRFLSLAPDEEVALTAEPKIDGLSCSLRYEK
                     GVLVLAATRGDGTTGEVVTENVRTIGDIPQRLEGEAPDVFEIRGEVYMSKADFAALNA
                     RLLAEAEDPARARQFANPRNAAAGSLRQKDAAVTASRPLRFYAHGWGEVSALPAETQL
                     GVMRAIHAWGLPIADHLERCADLEALLAHYRMIESKRADLAFDIDGVVYKVDRLDWQR
                     RLGQVARAPRWALAHKFPAEKAETLLEAIDIQVGRTGKLTPVARLRPVSVGGVTVTNA
                     TLHNKDEIERLGVRVGDRVRIQRAGDVIPQVLENLTPHEERPAYLFPTACPECGSDAV
                     REEGEVDVRCTGGLVCPVQRVERLRHFVSRHALDIEGLGITHIENFFRDGLLESPADI
                     FRLTREKLLSRERWAEISAANLIAAIDAKRQPPLDRFLYGLGIRHVGEVTARDLARRY
                     RSWDNFRSMIEAALKVRREIEPIEGEEERKLLQRRDKAVVEAIETPNIGPEVANALLD
                     FFEEAHNREVLDDLLAAGVRPADVVWETKASPVSGKTLVFTGSLETLSRDEAKAQAEK
                     LGAKVAGSVSARTDLVVYGPGAGSKLKKAAELGIETIDEAAWNALVKEAG"
     gene            complement(2032..2967)
                     /locus_tag="VGB57_11950"
     CDS             complement(2032..2967)
                     /locus_tag="VGB57_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0132107.1"
                     /translation="MTDSWKVSLPCTKAEAEALKDDISPLAWLDFPPVLMTSEAGGAR
                     PDEWRLDAYFDREPSAEMLGLLASLAPSAAGKAPAVEKVEDRDWVTLSQQGLEPIRAG
                     RFFVHTPAHRDGAPTYLIALEIDAGRAFGTGQHETTTGCLVALSRLKAGGAAVADLVD
                     LGTGTGLLAFAALKLWPAARAAASDNDPVAIEVSEENAAINGVRLGRARGQLELLVAE
                     GMDHPRLQARAPFDLVIANILAGPLIELAPSVATAIAPGGRLILAGLLDHQAPAVAAA
                     YRRQGMMLTGRVERDEWPTLVMRKRRAAPVRKRRD"
     gene            complement(3100..3798)
                     /locus_tag="VGB57_11955"
     CDS             complement(3100..3798)
                     /locus_tag="VGB57_11955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0132108.1"
                     /translation="MANILVTGTSRGIGKAIAAALSGHKVAGHSSKGGDGMIAADLSA
                     PGAAGRLWHEALERLGGRIDALVNNAGIFEAAPVDLPAEEWTAQWERTMRVNLTASAE
                     LCRLAVLHFREQGGGRIVNVASRAAYRGDSPQHWHYAASKAGMIGMTKSIARGYASDN
                     ILAFAVCPGFTASDMVEDYMASRGGEKIVADIPLGRVASADEVAETVRWLAVDAPASA
                     TGAVIDVNGASYVR"
     gene            complement(4008..>4810)
                     /locus_tag="VGB57_11960"
     CDS             complement(4008..>4810)
                     /locus_tag="VGB57_11960"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY0132109.1"
                     /translation="LCAHHDALRAGVGEPPIDRARLENDLAEIAEFVLAFARPVWKTL
                     GEARASGKRILFEGAQGVLLDVDHGTYPFVTSSNTVAGTAAGGSGLGPGAAGFVLGIV
                     KAYTTRVGAGPFPTELEDETGQRLGERGREFGTVTGRKRRCGWFDAVLVRQACAVAGV
                     TGVALTKLDVLDGFEELRICTGYRLRGADLDHLPAHAADQQAVEPVYETIPGWAESTA
                     GARSWAELPAQAIKYIKRIEELCRISVSLVSTSPEREDTILVRDPFAD"
BASE COUNT          736 a         1675 c         1693 g          706 t
ORIGIN      
        1 ccggagatat cggacgccga atatgacgcg ctggtccgcc gcaatgcgga gctggaagcc
       61 gccttccccc acctggcccg ccccgacagc ccctccagag cggtcggggc cgcgcccgcg
      121 gcgcatcttt ccaaggtccg ccacgcgctg ccgatgctca gcctcgagaa cgccttttcg
      181 gacgaggagg tcgccgattt catggcccgc gtccggcgct tcctcagcct cgcgccggac
      241 gaggaggtcg ccctcaccgc cgagcccaag atcgacggcc tgtcctgctc gctgcgctac
      301 gagaaaggcg tgctggtcct cgccgccacc cgtggcgacg gcacgaccgg cgaggtcgtc
      361 accgaaaatg tccggaccat cggcgacata ccgcagcggc tggagggcga agcgccggac
      421 gtcttcgaga tacgcggcga agtctacatg tcgaaagccg acttcgccgc cctcaatgcc
      481 cgcctgctcg ccgaagcgga agatccggcc agggcgcgcc agttcgccaa cccgcgcaac
      541 gccgccgcgg gctcgctccg gcagaaggac gccgcggtga ccgcctcccg gccgctgcgc
      601 ttctacgccc atggctgggg agaggtatcg gccctacccg ccgaaaccca gctcggggtg
      661 atgcgcgcga tccacgcctg ggggctgccg atcgccgacc acctcgaacg ctgcgccgat
      721 ctcgaggccc tgctcgccca ttaccggatg atcgagtcga agcgcgccga cctcgccttc
      781 gacatcgacg gggtcgtcta caaggtcgac cggctcgact ggcagcgccg tctcggccag
      841 gtggcgcgag cgccgcgctg ggcgctggcc cacaaattcc cggccgagaa ggcggagaca
      901 ttgctggaag ccatcgacat ccaggtcggc cggaccggca agctgacgcc ggtggctcgc
      961 ctcaggccgg tcagcgtcgg cggagtgaca gtcaccaacg cgaccctgca caacaaggac
     1021 gagatcgagc gcctgggcgt gcgggtcggg gaccgggtca ggatccagcg cgccggcgac
     1081 gtcatcccgc aggtgcttga gaatctcacc ccccatgagg agcgcccggc ctacctgttc
     1141 ccgaccgcct gcccggaatg cggctccgac gcggtgcgcg aggaaggcga ggtagacgtt
     1201 cgctgcaccg gcggcctcgt ctgcccggtc cagagggtcg agcggctgcg ccacttcgtc
     1261 tcccgccatg ccctcgacat cgaagggctc gggatcaccc atatcgagaa tttcttccgc
     1321 gacggcctgc tggaatcgcc ggccgacatc ttccggctca cccgggagaa attgctctcc
     1381 cgcgagcgct gggccgaaat ctcggcggcg aatctgatcg ccgcgatcga cgcgaaacgg
     1441 cagccgccgc tcgaccggtt tctctatggg ctgggaatcc gtcatgtcgg cgaggtgacg
     1501 gcccgcgacc tcgcccgccg ctaccggagc tgggacaatt tccgctcgat gatcgaagcg
     1561 gctctgaagg tgcggcgcga gatcgagccg atcgagggcg aggaggagcg caagctcctc
     1621 cagcgccgcg acaaggcggt ggtcgaagcg atcgagactc cgaacatcgg gccggaagtg
     1681 gccaacgccc tgctcgactt cttcgaggag gcgcataacc gggaggtgct cgacgacctg
     1741 ctcgccgccg gagtccgccc ggcggacgtg gtgtgggaga cgaaggcgtc gcccgtctcc
     1801 ggcaagaccc tggtcttcac cggaagcctc gagactctct ctcgcgacga agccaaggcg
     1861 caggccgaga agctcggcgc caaggtcgcg ggctcggtct cggccaggac cgacctggtg
     1921 gtctacggac ccggcgccgg atccaagctc aagaaggcgg cggagctggg catcgagacg
     1981 atcgacgagg cggcgtggaa cgccctggtg aaggaggcgg gataacgggg gtcagtcgcg
     2041 cctcttccga accggcgccg cccgccgctt gcgcatcacc agggtcggcc actcgtcgcg
     2101 ctcgacccgt ccggtcagca tcatcccctg gcggcgatag gcggcggcca ccgccggcgc
     2161 ctggtggtcg agcagccccg cgaggatcag ccttccgccc ggggcgatcg ccgttgcgac
     2221 cgagggcgct agctcgatca gcggtccggc gaggatgttg gcgatgacga gatcgaaggg
     2281 cgcccgggcc tgcaggcggg gatggtccat tccttcggcg acgagaagct cgagctggcc
     2341 gcgcgcccgc cccagcctga cgccattgat cgccgcattc tcctcgctca cctcgatcgc
     2401 caccggatcg ttgtcggacg cggccgcccg ggcggcgggc cacaatttga gggcggcgaa
     2461 ggcgaggagg ccggtgccgg tgccgagatc gacgagatcg gcgaccgccg ccccgcccgc
     2521 cttgagccgg ctgagggcga cgaggcagcc ggtcgtcgtc tcgtgctggc cggtgccgaa
     2581 ggcgcgtccc gcgtcgatct cgagcgcgat caggtaagtc ggggcgccgt cccgatgagc
     2641 gggcgtgtgg acgaagaagc gtccggcccg gatcggctcg agcccctgct ggctgagggt
     2701 cacccagtcg cggtcctcga ccttctccac cgccggcgcc ttcccggctg cgctcggcgc
     2761 cagcgaggcg agcaggccca gcatctcggc gctcggctcg cggtcgaaat aggcgtcgag
     2821 ccgccattca tcgggccgcg caccgcccgc ctcgctcgtc atcagcaccg gcgggaaatc
     2881 gagccaggcg agcggcgaga tgtcgtcctt gagcgcctcc gcttccgcct tggtgcaggg
     2941 cagtgagacc ttccagctgt cggtcatgtg aaatcctccc ctgcattcgc aggggagggg
     3001 gaccaggcga agcctggtgg aggggctttc cagcgcagaa gaacccctcc accgcgcttc
     3061 gcgcggtccc cctccccgcg aaatcgcggg gaggattttc taacgcacat agctcgcccc
     3121 gttgacgtcg atcaccgcgc cagtcgccga agcgggcgcg tccacggcca gccagcgcac
     3181 ggtctccgcg acctcgtcgg cgctggcgac ccggccgagc gggatgtcgg cgacgatctt
     3241 ctcgccgccg cggctcgcca tataatcctc gaccatgtcg gaggcggtaa agccggggca
     3301 gacggcgaag gcgaggatgt tgtcggaggc atagccgcgg gcgatcgact tggtcattcc
     3361 gatcatcccg gccttggagg cggcataatg ccagtgctgc gggctgtcgc cgcgataggc
     3421 ggcgcggctg gcgacgttga cgatccggcc gcccccctgc tcgcggaaat ggaggacggc
     3481 gaggcggcag agctcggccg aggcggtgag gttgacccgc atcgttcgct cccattgcgc
     3541 ggtccattcc tccgccggaa ggtcgaccgg ggcggcttcg aagatacccg cattgttgac
     3601 cagggcgtcg attcggccac cgagccgctc cagcgcctcg tgccagagcc gcccggccgc
     3661 gcccggggcg gagaggtcgg cggcgatcat tccgtcgccg cctttgctgg agtggccggc
     3721 gactttgtgg ccggagaggg cggcggcgat cgccttgccg atgccgcggc tggtgccggt
     3781 gacgaggatg ttagccatgg cggcgcgata ggcgcgggcg gggcggaggg gaaggcatct
     3841 ttgcctcccc atcgtaagat ggggaggggg accgcacgaa gtgtggtgga ggggtagatg
     3901 gctaccagcc cagcggttca gtcgccttag cctcggcttt cgcgccagtt accccgccac
     3961 caggcttcgc ctggtccccc tccccatgct ttgcacgggg aggcagatca gtccgcgaac
     4021 gggtccctca ccaaaatcgt gtcctcccgt tccgggctgg tcgacaccag ggagaccgag
     4081 atccggcaca attcctcgat gcgcttgata tatttgatcg cctgcgccgg aagctccgcc
     4141 cagcttcggg cgcccgcggt cgattccgcc catccgggga tggtctcgta gaccggctcg
     4201 accgcttgct ggtcggcggc atgggctggg aggtggtcga ggtcggcgcc gcgcaggcgg
     4261 tagccggtgc agattcggag ctcctcgaag ccgtcgagca cgtccaattt ggtcagcgcc
     4321 accccggtca ccccggcgac ggcgcaggcc tggcggacca gcaccgcgtc gaaccagccg
     4381 cagcggcgct tgcggccggt gacggtgccg aactcgcgcc cgcgctcgcc gagcctctgg
     4441 ccggtctcgt cctcgagctc ggtggggaag gggccggcgc cgacccgggt ggtgtaggcc
     4501 ttgacgatcc cgagcacgaa gccggccgcg cccggcccga ggccgcttcc gccggcggcg
     4561 gtgccggcga cggtgttgga cgaggtgacg aacggatagg tgccgtggtc gacgtcgaga
     4621 agcacgccct gagcgccttc gaacaggatg cgcttgccgc tcgcccgagc ctcgcccaac
     4681 gtcttccaga ccgggcgggc gaaggccagg acgaactcgg cgatctcggc aaggtcgttc
     4741 tcgagccggg cgcggtcgat cggcggttcg ccgacgccgg cccgcagcgc gtcgtgatgg
     4801 gcgcagagcc
//
LOCUS       DASWNU010000600         5714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_491830, whole genome shotgun sequence.
ACCESSION   DASWNU010000600 DASWNU010000000
VERSION     DASWNU010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491830"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VGB57_11965"
     CDS             complement(<1..319)
                     /locus_tag="VGB57_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0132110.1"
                     /translation="MSETILITGGAGFIGRAVTRELVGRGHRVRILDSLIEQVHGDRG
                     HFDGAAMDAELIVGDVRNGDVVTRALQGVDSVIHLAAEVGVGQSMYEVERYTSVNDVG
                     TAVL"
     gene            624..1223
                     /locus_tag="VGB57_11970"
     CDS             624..1223
                     /locus_tag="VGB57_11970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0132111.1"
                     /translation="MTATILLVRHAAHVELGAILSGRRRDVALSPEGLEQAAILGDLL
                     GTAPIAAVYSSPRERAWYTAREIAEPHGLKPAILDDLDELDFGEWTGRSFAELDALSE
                     WQLWNSARATARPPDGESMAEAVGRASSALDGLVADHDGETIVAVSHCDVIRGIIARH
                     LGLGLDHLLRFDVDPASVSRLTLGNWGARINSINERLYQ"
     gene            1220..2017
                     /locus_tag="VGB57_11975"
     CDS             1220..2017
                     /locus_tag="VGB57_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HEY0132112.1"
                     /translation="MNLAGRRIEPAGDGEVKARIEALGPWFQNMDLGGVWTAPDHFLG
                     DYPGEKFQRFAPHLPADLEGRSVLDIGCNAGFYSIEMKRRGAGRVLGIDSDERYLAQA
                     RFASERLGFADIEYRQLDVYDVGAIGERFDLVIFMGVLYHLRHPLLALDLIREHVAGD
                     MLLFQSMQRGSRQLLEVEGDYDFNQTEHFFESGYPKLHFIEHQYAGDWTNWWVPNRAC
                     AEAMLRASGFEIETRAEEEVYLCRKAARPFEDWGGGAPVYPARGDGE"
     gene            2014..2928
                     /locus_tag="VGB57_11980"
     CDS             2014..2928
                     /locus_tag="VGB57_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEY0132113.1"
                     /translation="MIEAAMIWNEPNNKSHWDPELDPDWSIFAETAIQAGKAIAAINP
                     AIVRVLGGMSPIDPAFVARLEAKGVLDHVDVIAVHGFPLDWNLWPIHEWPDRIAEIEA
                     AAPGHDIWVTEVGVSSFGAEEVQVFGLDRTAELLLGRVPRVYWYSLFDLPRAWEATTR
                     HKEAEGSSYYRHFYMGLIREDGTPKPALEHFARHSPEMGLMQWFHFEDPRLDEAVAWM
                     KRLGVRKLRTGLSWWDSYRPGWEDWFDRQMDALAEFDTTVTFCFTPQHLGLQPHHTSP
                     PREPAYFADFCARMIERYAPAERRQKVA"
     gene            complement(2943..3019)
                     /locus_tag="VGB57_11985"
     tRNA            complement(2943..3019)
                     /locus_tag="VGB57_11985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2983..2985),aa:Arg,seq:ccg)
     gene            complement(3078..3467)
                     /locus_tag="VGB57_11990"
     CDS             complement(3078..3467)
                     /locus_tag="VGB57_11990"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEY0132114.1"
                     /translation="MSMRVAGLAALALLGGCAQGSTERQEANRRALAEATPVGEPVDC
                     IELTRIDRTDVRDNRTIDFHMRGREVYRNRLRHECPGLAFEESFTYRPSTGRLCSVDL
                     IAVNSRTGIAGPTCALGSFQRIETSAR"
     gene            complement(3464..4150)
                     /gene="nth"
                     /locus_tag="VGB57_11995"
     CDS             complement(3464..4150)
                     /gene="nth"
                     /locus_tag="VGB57_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0132115.1"
                     /translation="MSPDEIATLYRRLHDRTPSPETELEFVNDYTLLVAVVLSAQATD
                     AGVNLATRDLFARASTPQAMVALGEEGLKEHIRTIGLFNTKAKNVIALSQALIERHGG
                     EVPEDFEALQALPGVGRKTANVVMNCAFGAETFAVDTHVFRVSNRTGLAPGKDVLEVE
                     RGLDAVTPPPFRRDAHHLLILHGRYVCKARKPECWRCTIADICRFEPKTPPPGSEAGA
                     GALKPARRRT"
     gene            complement(4147..4845)
                     /gene="dapB"
                     /locus_tag="VGB57_12000"
     CDS             complement(4147..4845)
                     /gene="dapB"
                     /locus_tag="VGB57_12000"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY0132116.1"
                     /translation="MTAISIFGAGGRMGQAIAEAAALRGDVRLSEHHPDVFVDFSAPE
                     AVEAHLRQARRAGRPILIGTTGLDSAHERLIGEASAEIPLIHAANTSLGVNLLAHLVR
                     ETAARLGEDWDLEIVEMHHRMKADSPSGTALMLGRAGAEGRGTDLDSVAERGRDGITG
                     PREPGRIGFASLRGGSVAGEHLAIFASEGERLELGHRAEGRAIFARGAIEAALWLVGK
                     PAGRYSMADVLGLK"
     gene            complement(4904..5464)
                     /locus_tag="VGB57_12005"
     CDS             complement(4904..5464)
                     /locus_tag="VGB57_12005"
                     /inference="COORDINATES: protein motif:HMM:NF045466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132117.1"
                     /translation="MPKGMRLFHFSDDPSIEVFEPRPVRVPSPREAGMEWLNGPLVWA
                     IDDWHQPLYLFPRECPRILIWPGPRTTPQDRAYWWGDRSGRMVAHVEAAWRDRLSAAR
                     LYRYELPRLAFEPLNDAGMFVSRVPVEPLGLEVIESLPSQLSACEVELEIMPSLRPLR
                     DVWKSSMSASGIRLRNAQDWTQSPTR"
     gene            5541..>5714
                     /locus_tag="VGB57_12010"
     CDS             5541..>5714
                     /locus_tag="VGB57_12010"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEY0132118.1"
                     /translation="MREIGNIVILTGAGISAESGLATFRGPDGLWEGHRVEDVATPQA
                     FRRDPALVQAFYDA"
BASE COUNT          960 a         1921 c         1839 g          994 t
ORIGIN      
        1 gcaggacggc ggtgccgacg tcgttgaccg aggtatagcg ctcgacctcg tacatggact
       61 ggccgactcc gacctcggcg gcgagatgga tgacggagtc gacgccctga agcgcccggg
      121 tgacgacgtc gccgttgcgc acatcgccta cgatcagctc ggcatccatg gccgctccgt
      181 cgaagtggcc gcgatcgccg tggacctgct cgatcagact atcgaggatt cggacgcgat
      241 gtccgcgccc gaccagctcc cgagtcaccg cccgtccaat gaagccggcc ccgccggtga
      301 tcaggatcgt ctcagacaat gcttcatcct tgctgtatca aaatctcaaa agaatgcaca
      361 aattcgttcc gcaattcacg tacggcgcat ctgttctaag gtggacataa gaataggttg
      421 gaaagctttc cgatatagat gccgcattta tctatgttta taatctgcgt tataatttca
      481 acagcttctt catgatcaac ccatctctgc atcatcttgt tccgctacca ttccgtttcg
      541 gtctgtcaac caactgaagg aacttaactt gtgtttggcc attcagcttt gtagatgctg
      601 gtccaatccg ccattcgatg ctcgtgaccg cgaccatcct cctcgtccgc cacgcggccc
      661 acgttgaact gggcgcgata ttgagcggcc gtcggcgcga cgtagcgctg agcccggagg
      721 gcctggagca ggcggcgatc ctcggcgacc ttctcggcac cgccccgatc gccgccgtct
      781 attcgagccc gcgggagcgc gcctggtata cggcgcgcga gatcgccgag ccgcatggcc
      841 tcaagccggc catcctcgac gatctcgacg agctcgactt cggcgagtgg accggccgca
      901 gcttcgcgga gctggacgcc ctgtccgaat ggcagctctg gaactcggcc cgggcgacgg
      961 ccaggccgcc ggacggcgag tcgatggccg aggcggtcgg ccgggcgtcc tcggcgctcg
     1021 acggcctcgt cgccgatcat gacggcgaga cgatcgtggc ggtcagccat tgcgacgtga
     1081 tccgcggaat catcgcccgt catctcggcc tcggcctcga ccatctgctc cggttcgacg
     1141 tcgatccggc ctctgtgtcg cggctgacgc tcggcaattg gggcgcgcgc atcaacagca
     1201 tcaacgagag gctctatcaa tgaacctggc cggaagacga atcgagcctg cgggcgatgg
     1261 cgaagtgaag gcgaggatcg aggcgctggg cccctggttc cagaacatgg atctcggcgg
     1321 agtctggacc gcgcccgatc attttctcgg cgactatccg ggcgagaagt tccagcgctt
     1381 cgcgccgcat ctgcccgccg atctcgaggg caggagcgtg ctcgacatcg gctgcaatgc
     1441 gggcttctat tcgatcgaga tgaagcgccg aggcgccggg cgggtgctcg gcatcgacag
     1501 cgacgaacgc tatctggccc aggcccggtt cgcgagcgag aggctcggtt tcgccgacat
     1561 cgaatatcgc cagctcgacg tctacgatgt cggagcgatc ggcgagcggt tcgatctcgt
     1621 catcttcatg ggcgtcctct accatttgcg gcatcccctg ctcgcgctcg acctgatccg
     1681 cgagcatgtc gccggcgaca tgctcctctt ccagtcgatg cagcgcggct cgcggcagct
     1741 gctcgaggtc gaaggggatt acgatttcaa ccaaaccgag catttcttcg agagcggcta
     1801 tcccaagctc cacttcatcg agcaccaata tgccggcgac tggaccaact ggtgggtgcc
     1861 caaccgcgcc tgcgccgagg cgatgctgcg cgcctccggc ttcgagatcg agactcgggc
     1921 cgaggaggaa gtctatctct gccgcaaggc cgcacggccg ttcgaggact ggggcggcgg
     1981 cgccccggtt tatcccgcga gaggagacgg cgaatgatcg aagccgcgat gatctggaac
     2041 gagcccaaca acaagtcgca ttgggatccc gagctcgatc cggactggtc gatcttcgcc
     2101 gagaccgcga tccaggccgg caaggcgatc gccgcgatca atcccgccat cgtgcgggtg
     2161 ctcggcggaa tgtctccgat cgatccggcc ttcgtcgccc gcctcgaggc caagggggtg
     2221 ctcgaccatg tcgacgtgat cgccgtccac ggcttcccgc tcgactggaa tttgtggccg
     2281 atccacgaat ggccggaccg gatcgcggag atcgaggcgg cggcgcccgg tcacgacatc
     2341 tgggtgaccg aggtcggcgt aagcagcttc ggcgccgagg aggtgcaggt gttcggcctc
     2401 gaccgaaccg ccgagcttct gctcggccgg gtgccgcggg tctattggta ttcgctgttc
     2461 gacctgcccc gcgcctggga ggcgaccacc cgccacaagg aggccgaggg ctcgtcttat
     2521 taccgccatt tctacatggg cctgatccgc gaggacggca cgcccaagcc cgcgctcgag
     2581 catttcgccc gccactcgcc cgaaatgggg ctgatgcaat ggttccactt cgaggacccc
     2641 cgcctcgacg aggccgtggc ctggatgaag cgactgggag tacgcaaatt gcgcaccggc
     2701 ctcagctggt gggacagcta ccggcctggc tgggaggact ggtttgatcg gcagatggac
     2761 gcgctcgccg agttcgacac gaccgtgact ttctgcttca cgccgcagca tctcgggctc
     2821 cagccgcacc ataccagccc gccgcgcgag ccggcctact tcgccgactt ctgcgcccgg
     2881 atgatcgaac gctacgcccc ggccgagcgc cggcagaagg tggcctgatc cgttgaggtg
     2941 attggcgcac ccaagaggat tcgaacctct ggcctctgcc ttcggagggc agcgctctat
     3001 ccagctgagc tatgggtgcg tggaagcggc gcttagcaaa ggggggcggc cgccgccagt
     3061 ggctttagcg ggcccgtcta acgagcactc gtctcaatcc gctggaacga ccccagggcg
     3121 caggtcgggc ctgcgatccc ggtgcggctg ttgaccgcga tcagatcgac cgagcagagc
     3181 cgcccggtcg acggccggta ggtgaagctc tcctcgaagg cgaggccggg gcattcgtgc
     3241 cggagccggt tccggtagac ctcgcgcccg cgcatgtgga agtcgatcgt ccggttgtcg
     3301 cggacgtcgg tacggtcgat ccgggtgagc tcgatgcagt cgaccggctc gccgaccggt
     3361 gtcgcctcgg cgagcgcccg ccggttcgct tcctggcgct ccgtcgatcc ctgagcgcag
     3421 ccgcccagaa gggccaatgc ggcaagtccg gcaaccctca tactcatgtc cttctcctcg
     3481 cgggcttcaa cgcaccggcg cccgcttcgc ttcccggcgg cggcgttttc ggctcgaagc
     3541 ggcagatgtc cgcgatcgtg cagcgccagc attccggctt cctcgccttg caaacgtagc
     3601 ggccgtggag gatgagcagg tggtgggcgt cgcggcggaa tggcggtggg gtgacagcgt
     3661 cgagcccccg ctccacctcc agcacgtcct tgcccggcgc gaggccggtc cggttggaga
     3721 cccggaagac atgggtgtcg acggcgaaag tctccgcccc gaacgcgcag ttcatgacca
     3781 cgttggcggt cttgcgcccg accccgggaa gcgcctgaag cgcctcgaaa tcctcgggca
     3841 cctcgccgcc gtgccgctcg atcagggcct gggagagggc gatcacgttc ttcgccttgg
     3901 tgttgaacag gccgatcgtc ctgatgtgct ctttcagccc ttcctcgccg agcgccacca
     3961 tcgcctgggg cgtgctcgcc ctggcgaaga ggtcgcgggt ggcgaggttg acgccggcgt
     4021 cggtcgcctg cgccgaaagg acgacggcga ccagcaatgt ataatcattc acgaactcga
     4081 gctcggtctc cgggctcggc gtacgatcat gcagccgcct gtataatgtt gcgatctcgt
     4141 cgggactcac ttcagcccca gcacgtccgc catcgagtag cggccggcag gcttgccgac
     4201 gagccacaag gccgcctcga tcgctccccg ggcgaagatc gcccggccct ccgcccgatg
     4261 gccgagctcg agcctttccc cctccgaggc gaagatcgcg agatgctcgc cggcgaccga
     4321 tccgccgcga agcgaggcga aaccgatccg ccccggctcg cgcggacccg tgatcccgtc
     4381 gcgaccgcgc tcggcgacag agtcgagatc ggtcccccgc ccttcagcgc cggcgcggcc
     4441 cagcatcaag gcggtgcccg acggcgagtc cgccttcatc cggtgatgca tctcgacgat
     4501 ctccaggtcc caatcctcgc cgagccgggc cgcggtctcg cgcaccaggt gggcgagcag
     4561 gttgaccccg agcgaggtgt tggcggcatg gatgagcggg atctcggccg aagcctcgcc
     4621 gatcaggcgt tcatgggcgg agtcgagacc ggtcgtaccg atcaggatcg gcctgcccgc
     4681 cctccgcgcc tgccgcagat gcgcctcgac cgcctccgga gcggagaaat ccacgaagac
     4741 gtcgggatga tgctcggaca gccggacgtc gccgcgaagc gccgccgcct cggcgatcgc
     4801 ctgccccatc cgcccgccgg cgccgaagat ggagattgcc gtcatcgccc cttccatgcg
     4861 ggagccgtcc ggagagcgca accgcccgct aagttcatga agttcatcgg gtcgggctct
     4921 gtgtccagtc ctgcgcgttt cggaggcgta tgccgctggc cgacatcgag cttttccaca
     4981 cgtcgcgaag cgggcgtagc gacggcatga tctcaagttc gacttcgcaa gccgagagtt
     5041 gcgatggaag gctctcgatg acctccagtc ccagcggctc aaccgggacg cgcgagacga
     5101 acatgccggc atcgttcagc ggctcgaacg ccaggcgggg caattcatag cgataaaggc
     5161 gcgccgcgct cagcctgtcc cgccatgccg cctccacgtg cgcaaccatg cgcccggaac
     5221 ggtcgcccca ccaataggcg cgatcctgcg gggtcgtccg gggtccgggc caaatgagga
     5281 tgcgcgggca ttctcgcgga aacaggtaga gtggctgatg ccagtcgtcg atcgcccaga
     5341 ccaacggccc gttgagccac tccattccag cttcccttgg cgaaggcact cgaactggcc
     5401 gcggctcgaa aacctcgatg gagggatcgt cgctgaagtg aaacagccgc atgcccttcg
     5461 gcatttacgt tctgtgcact tcgaagtgaa tgtctgcttt cgacttagcg gacttccccg
     5521 atagcggcta aagcggctcc atgcgcgaga tcggaaacat cgtcatcctg accggcgccg
     5581 gaatctcggc ggagagcggc ctcgcaacct tccgcgggcc cgacgggctt tgggagggcc
     5641 accgggtcga ggacgtggcc acgccccaag ccttccgccg cgacccggcg ctggtccagg
     5701 ccttttacga cgcc
//
LOCUS       DASWNU010000601         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_153677, whole genome shotgun sequence.
ACCESSION   DASWNU010000601 DASWNU010000000
VERSION     DASWNU010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153677"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGB57_12015"
     CDS             complement(<1..205)
                     /locus_tag="VGB57_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0132119.1"
                     /translation="MAVLAGGRSSEHEVSLRSGAAVARGLREGGHEPVEVTIGRDGRW
                     SAAGAAVELTPGAGLLGADVAFPA"
     gene            complement(208..>1610)
                     /locus_tag="VGB57_12020"
     CDS             complement(208..>1610)
                     /locus_tag="VGB57_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY0132120.1"
                     /translation="FGIARAGVSEITQAGSVMGTPHYLSPEQAQGFDVTPVSDLYSIG
                     VILYEALTRRVPFEGESAVAVAMKQVSQVPQRPSSVNPQVSPALDAVVMRALEKDPGQ
                     RFQSADAFIAALDAALKDPGAGQGTAAFAAAPPVLEEEGPLEDRKRNWWLWGLLVGAI
                     LIGLLVGLALTRDTKTEVPNVTGNQLGVAITLLEQDGFTVGDVTRVEREAPANEVLEQ
                     DPAASPPQASLDCVFITFFCSKPEVALTVSSGPGSAKVPSTAGQTEEEAASRLEGAGF
                     EVDVTRVNSTAVESGLVIYSQPRGGNTATKGSTVVLFVSRGPKLTKVPVLVGSQRGAA
                     VERLRGRGLEASISEEESSSPEGQVISQSPEAGSEVEAGATVSLVVSSGEEEEEQARV
                     PNVIGKLRREAVEALRAAGLQPSVEEEETSVAGKVGRVTDQFPPPGTEAEPGETVTIT
                     VGKAAVEEVEEEGLEE"
BASE COUNT          202 a          593 c          562 g          253 t
ORIGIN      
        1 gggccgggaa ggcgacgtcg gccccgagca ggccggcgcc gggggtcaac tcgaccgcgg
       61 cgccggcggc gctccagcgc ccgtcgcggc cgatcgtcac ctcgaccggc tcgtggcccc
      121 cctcgcgcag gccgcgcgcc accgccgcgc cggagcgcag cgagacctcg tgctcggagg
      181 agcgcccacc ggccagcacc gccaccctca ttcttctaat ccttcttctt cgacctcttc
      241 gaccgcggct ttgccgacgg tgatcgtcac cgtttcgccc ggttcggctt cggtccccgg
      301 cggcgggaac tggtcggtga cgcggcccac tttgccggcc accgaggttt cctcttcttc
      361 gacgctcggc tgcagcccgg cggcgcggag cgcttccacc gcctcgcgac gcagctttcc
      421 gatcacgttc gggacccgcg cctgctcctc ctcttcttca ccgctggaga cgacgaggga
      481 gacggtcgcg cccgcttcga cttcgctgcc cgcctccggc gactggctga tcacctggcc
      541 ctcgggagac gagctttctt cttcggagat gctcgcttcc agcccccgcc cgcggagtcg
      601 ttcgaccgcc gccccgcgtt ggctgccgac caggaccggc accttggtca gcttcggccc
      661 gcgggagacg aagaggacga cggtggaacc tttggtcgcg gtgttgccgc cgcgcggttg
      721 cgagtagatc accaggcccg attcgaccgc ggtcgagttg acccgggtga cgtcgacttc
      781 gaagccggcc ccctcgagcc ggctcgcggc ctcttcctcg gtctgcccgg cggtcgaggg
      841 gaccttggcg ctgcccggcc cggagctgac cgtcagcgcc acctccggct tcgagcagaa
      901 gaaggtgatg aagacgcagt cgagcgaggc ctggggcggc gaggccgccg ggtcctgctc
      961 gaggacttcg ttggcgggcg cttcgcgctc gacccgggtc acgtcgccga ccgtgaagcc
     1021 gtcctgctcg agcagggtga tcgcgacccc gagctggttg ccggtcacgt tcggaacctc
     1081 ggtcttggtg tcgcgggtga gcgcgaggcc gacgagcagg ccgatcagga tcgcccccac
     1141 gagcaacccc cagagccacc agttgcgctt cctgtcttcg agcgggccct cctcctcgag
     1201 gacggggggc gcggcggcga aggcggcggt gccctgaccg gcgccggggt ctttgagcgc
     1261 cgcgtcgagg gcggcgatga aggcgtcggc gctctggaac cgctggcccg ggtccttctc
     1321 cagcgcccgc atcaccaccg cgtcgagcgc gggcgagacc tgtgggttga ccgagctcgg
     1381 ccgctgcggc acctgcgaga cctgtttcat cgccaccgcc accgcgctct cgccctcgaa
     1441 ggggacgcgg cgggtcagcg cctcgtagag gatcaccccg atcgagtaga ggtcggagac
     1501 cggggtgacg tcgaagccct gcgcctgctc gggcgagagg tagtgcggcg tccccatcac
     1561 cgagccggcc tgggtgatct ccgagacgcc ggcgcgggcg atcccgaagt
//
LOCUS       DASWNU010000602         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_308088, whole genome shotgun sequence.
ACCESSION   DASWNU010000602 DASWNU010000000
VERSION     DASWNU010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308088"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGB57_12025"
     CDS             complement(<1..534)
                     /locus_tag="VGB57_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132121.1"
                     /translation="MRNLVLAAALAALASPAAAAPAAPVPARAMTCPIGGGSFQFQPV
                     APPVVAGERPDGRPIGGVKFPLPLPECPDNGLVLYKDYEPAEVAKIEPMVASEAYQAL
                     RKDGTQYYRAYWLMKEMGVRPELYLWALLQASWEAEDRPELRKRYLEELVEATAKVPQ
                     RPSDLAWIGMEGRAINAL"
     gene            complement(564..962)
                     /gene="apaG"
                     /locus_tag="VGB57_12030"
     CDS             complement(564..962)
                     /gene="apaG"
                     /locus_tag="VGB57_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY0132122.1"
                     /translation="MKSLFPHSATTGDVTVRVSVSFLPEQSEPGRGRWFWAYHVRIEN
                     GGRRAVQLISREWTIADGRGGRHQVQGEGVVGEQPVIEPGGSFDYVSGCPLPTPTGWM
                     EGHYQMVSADGRGFDAAIPRFPLVAPAVTS"
     gene            1038..>2108
                     /locus_tag="VGB57_12035"
     CDS             1038..>2108
                     /locus_tag="VGB57_12035"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY0132123.1"
                     /translation="MAQDWTPASWRAHEARHQPVYDDPAALDAALRELAAYPPLVPSA
                     EAHALKAELAEAQAGRAFLLQGGDCAESFAEFSTETIRGTAALIEAMARRLAAASGLR
                     IVRVGRIAGQFAKPRSRELESKDGLDLPMYRGDIVNGLAFDAAARRPDPERMFRAYGQ
                     SAAALSHLRAFAGPEPIYTSHEGLLLPYEQALTRRDGAGWYASSAHFLWVGDRTRFAG
                     SAHVEYLRGLTNPIGIKCGPTLGADLLIELLETLNPAREPGRVTLVSRMGAGEVERAL
                     PPLLRAVAAEGHPVLWTCDPMHGNTRTVGAIKTRHFDDVLDEVLGFFEVHRNLGTWPG
                     GLHVEFTGEDVTECLGGADEVLE"
BASE COUNT          319 a          771 c          705 g          313 t
ORIGIN      
        1 gagcgcgttg atcgcccgcc cctccattcc gatccaggcg aggtccgaag ggcgctgcgg
       61 caccttcgcc gtggcttcga ccagctcctc cagatagcgc ttcctgagct ccggcctgtc
      121 ctcggcctcc cagctcgcct ggagcagcgc ccaaagatag agctccggcc tgacgcccat
      181 ctccttcatc agccaatagg cccggtaata ttgggtgccg tccttgcgga gcgcctgata
      241 ggcctcggag gccaccatcg gttcgatctt cgcgacctcg gcgggctcat aatccttgta
      301 gagcacaagc ccgttgtccg gacattccgg aaggggcagc ggaaacttca ctccgccgat
      361 cggacggccg tccggccgct ccccggcgac gacgggcggc gcgacgggct ggaactggaa
      421 gctgccgccg ccgatcgggc aggtcatcgc tcttgccggc acaggcgcgg cgggggcggc
      481 ggcggcggga gaggcgagag cggcaagtgc cgcggcaagg actaggttac gcatcgcaat
      541 actcctggga cgccgggccc cttttagctc gtgaccgcgg gcgcgacgag agggaagcgc
      601 ggaatggcgg cgtcgaaccc tcgcccgtcc gcggagacca tctgataatg gccttccatc
      661 cagcccgtgg gcgtgggcaa cggacagccc gagacatagt cgaacgaccc tccgggctcg
      721 atcaccggct gctcgccgac caccccctcg ccctggacct ggtgccggcc gccgcgcccg
      781 tcggcgatcg tccattcgcg gctgatcagc tggaccgcgc ggcggccgcc attctcgatc
      841 cggacatgat aggcccagaa ccagcggccg cggcccggct cggactgttc ggggaggaag
      901 ctgaccgaca cccggacggt gacgtcgccg gtcgtggcgc tatggggaaa gaggcttttc
      961 acaagcgctt ccctctactt atcggccgca cgcgtcaatc gcttgcacgg ccggacgcga
     1021 tccgcgaagg ggttgtcatg gcgcaggact ggaccccggc aagctggcgc gcgcacgagg
     1081 cgcggcacca gcccgtctac gacgatcccg ccgccctcga tgccgccctg cgcgagctcg
     1141 ccgcctaccc gcctttggtg ccgagcgccg aggcccatgc cctgaaggcc gagcttgccg
     1201 aggctcaggc cggccgagcc ttccttctcc agggcggcga ctgcgccgaa agcttcgccg
     1261 aattctcgac cgaaacgatc cggggcaccg ccgccctgat cgaggcgatg gcccggcgcc
     1321 tcgcggcggc gagcgggctt cggatcgtcc gggtcggccg aatcgccggc cagttcgcca
     1381 agccgcgctc gcgcgagctg gaatcgaagg acgggctcga cctgcccatg taccgcggcg
     1441 acatcgtcaa cggcctcgcc ttcgacgccg ccgcccgccg gcccgacccg gagcggatgt
     1501 tccgcgccta cggccagtcg gcggccgcct tgagccattt gcgcgccttc gccgggccgg
     1561 agccgatcta caccagccac gaaggcctgc tcctccccta cgagcaggcg ctgacccgcc
     1621 gcgacggagc cggctggtac gcaagctcgg cccatttcct ctgggtcggc gaccgcaccc
     1681 gcttcgccgg ctcggcccat gtcgaatatc ttcgcggcct caccaacccg atcggcatca
     1741 aatgcggccc caccctcggt gcggacctgc tgatcgagct gctcgagacc ttgaacccgg
     1801 cgcgcgagcc gggacgggtg accctggtca gccggatggg cgcgggcgag gtcgaacggg
     1861 cgctgccgcc tttgcttcgg gccgtcgccg ccgagggcca tccggtgctt tggacctgcg
     1921 atccgatgca cggcaacacc cgcacggtcg gcgcgatcaa gacgcggcac ttcgacgacg
     1981 tgctcgacga ggtcctcggc ttcttcgagg tgcaccgcaa cctcggcacg tggccgggcg
     2041 ggctgcacgt cgagttcacc ggtgaggacg tcaccgaatg cctcggcggg gcagacgagg
     2101 tcctcgag
//
LOCUS       DASWNU010000603         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_463552, whole genome shotgun sequence.
ACCESSION   DASWNU010000603 DASWNU010000000
VERSION     DASWNU010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463552"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2687
                     /locus_tag="VGB57_12040"
     CDS             <1..2687
                     /locus_tag="VGB57_12040"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY0132124.1"
                     /translation="KVAAKAKEAAVASGKSTADVEQAANDSICAMMLIRTYRVRGHLA
                     ANLDPLGLAKREMPEDLTPEYHGFTGAALDRTVWLGGALGLKQGTVREVVEILRRNYC
                     GNVGLEYMHINDLDERRFLQERLEGRDAEIRFTPEGKQSILQKVIEAEQWEKFLARKY
                     VGTKRFGLDGGESMVPALEAVIKYGGQYGVREIVFGMAHRGRLNVLANVMAKPYRAIF
                     SEFAGGSANPEDVGGSGDVKYHLGTSSDREFDGIQVHMSLVPNPSHLEAVDPVVLGKV
                     RALQVGRGGSKAVGDVLPVLLHGDAAFAGQGIVAECFAFSGIPGYGTGGTIHYVINNQ
                     VGFTTSPQFARSSPYPSDIAKSVQAPILHVNGDDPEAVTFACKLAIEYRQKFARDIVI
                     DMWCYRRFGHNEGDEPSFTQPLMYAEIRKHPPVSELYGARLREEGVIDPAWAEARVGE
                     FTALLENEFEAAKSYLPNKADWFEGRWSGLGRPETPETERRNVSTGVDENTLREVGAI
                     LTSIPEGLTVHKTLQRIIDAKAEMFASGRGFDWATAEALAFGTLLRDGCGVRLSGQDS
                     ARGTFSHRHAAWVDQTNGHKYIPLTRVEGARFEVRDSPLSEFGVLGYEYGFSIADPHT
                     LVLWEAQFGDFANGAQVIIDQFIAAGEAKWLRASGLVMLLPHGYEGQGPEHSSARPER
                     FLQLCGEDNMQVAYCTTPANYFHLLRRQMKRDFRKPLIVMTPKSLLRHKLAVSGVADF
                     TGESHFKRILSDVGGPADAEVKRLVLCSGKIAYELMESRDQAEDRHTSIVRLEQLYPF
                     PSEPLTTRLKRMTALEEVVWAQEEPQNQGYWNFLDWRIERCLEEAGVKPRRALYAGRP
                     ASASPATGLAKRHAAEQAALIADALGHSSHAPAQRKAG"
     gene            2697..3983
                     /gene="odhB"
                     /locus_tag="VGB57_12045"
     CDS             2697..3983
                     /gene="odhB"
                     /locus_tag="VGB57_12045"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY0132125.1"
                     /translation="MATEVKVPTLGESITEATLGQWLKMPGDAVKADEPVASLETDKV
                     AIEVPSPVDGVMGDQLVAVGDTVAVGAAIARIESGSGMPATAQSEQATPAAVPVNPEG
                     AGENVELRSDPAESAEDDDGHNLTLSPAVRRIVLEHHLDPSRIKGTGKDGRLTKDDVL
                     AAAAKAKEAPAPAPAAPPPPPPPAAAAPAPRPPSGGRREERVRMTRLRQTVARRLKEA
                     QNTAALLTTFNDVDMTAVMEARSRYKDLFEKKHGIRLGFMSFFVKAAVLAARDVPSVN
                     AYIEGEEIVYHDYVDVSVAVSAPNGLVVPVIRDAQALSFAEVEKTIAEFGKRAKEGSL
                     TMADMEGGTFTISNGGVFGSLLSTPIINPPQSAVLGMHRIEERPVVRDGQVVVRPMMY
                     LALSYDHRLVDGREAVTFLVRMKEALEDPTRLLIDL"
     gene            4260..4550
                     /locus_tag="VGB57_12050"
     CDS             4260..4550
                     /locus_tag="VGB57_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0132126.1"
                     /translation="MTKALDRTPWDGIIVTDADTCFGKARIAGTRIYVDFILQAIEGG
                     WSIDDFVREYPHVTRAQVQAVIGFARDLVAAKRNRLKGEQRRAAPAEEIEQA"
     gene            4547..5944
                     /gene="lpdA"
                     /locus_tag="VGB57_12055"
     CDS             4547..5944
                     /gene="lpdA"
                     /locus_tag="VGB57_12055"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0132127.1"
                     /translation="MTDTQFDVIIIGAGPGGYVAAIRAAQLGLKTACVESRETLGGTC
                     LNVGCIPSKALLHASELFEEAAHGTMAKWGIKATGVELDLDVMHAGRKEAIKGLTGGI
                     EFLFKKNKITWLKGHAKFTGPNAIDVAGQAYSAKSIVIATGSSVTPLPGVEIDQERIV
                     DSTGALELARVPGHMVVIGGGVIGLELGSVWRRLGARVTVVEYLDQLLPGMDGEVRKE
                     AGKIFKKQGFEIRTSTKVTKAEKTGEGVALTLEPAQGGEAQTLEADVVLVSIGRRPNV
                     DGLNLEAAGIQLNRRGQIEVDHEFRTGVEGVYAIGDVTPGPMLAHKAEDEGIAVAEIL
                     AGQVGIINHDVIPGVVYTYPEIASVGKTEEELKEAGVEYKTGKFPMMANSRAKTNRDT
                     DGFVKVIADARTDRVLGVHIIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEA
                     MKEAAMAVTGLPIHI"
BASE COUNT         1112 a         1940 c         2035 g          945 t
ORIGIN      
        1 agaaggtggc ggcgaaggcg aaggaggccg ccgtcgcgag cggcaagtcg acggcggacg
       61 tcgagcaggc ggccaacgac agcatctgcg cgatgatgct gatccgcacc taccgggtgc
      121 gcgggcatct cgccgccaat ctcgatcccc tcggcctcgc caagcgcgag atgcccgaag
      181 acctgacgcc cgaatatcac ggcttcaccg gagccgcgct cgaccggacg gtgtggctcg
      241 gcggagcgct cggactgaag cagggcacgg tccgcgaggt cgtcgagatc ctgaggcgca
      301 attattgcgg caatgtcggc ctcgaatata tgcacatcaa cgacctcgac gagcgccgct
      361 tccttcagga gcggttggag ggccgggacg ccgagatccg gttcactccc gaaggcaagc
      421 agtcgatcct ccagaaggtg atcgaggccg agcagtggga gaagttcctc gcccgcaaat
      481 atgtcggcac caagcgcttc ggcctcgacg gcggcgaatc gatggtcccc gcgctcgaag
      541 ccgtgatcaa atatggcggc cagtatggcg ttcgcgagat cgtgttcggg atggcccatc
      601 gcgggcggct gaacgtcctc gccaacgtca tggcgaagcc ctaccgcgcg atcttcagcg
      661 agttcgccgg aggctccgcc aatcccgagg acgtcggcgg ctcgggcgac gtcaaatatc
      721 atctcggaac ctcgtcggac cgcgagttcg acgggatcca ggtccatatg tcgctggtcc
      781 ccaatcccag ccatctcgag gcggtggacc cggtcgtgct cggcaaggtg cgggcgctcc
      841 aggtcggtcg cggcggaagc aaggccgtgg gcgacgtcct gccggtgctt ctgcacggcg
      901 acgccgcctt cgccggccag gggatcgtcg ccgaatgttt cgccttctcc gggatccccg
      961 gctacggcac cggcggcacg atccactacg tcatcaacaa ccaggttggt ttcacgacct
     1021 ccccgcaatt cgccagatcg tccccatatc cgtccgacat cgccaaatcg gtccaggcgc
     1081 cgatcctgca cgtcaacggc gacgatcccg aagcggtgac cttcgcctgc aagctggcca
     1141 tcgaatatcg gcagaagttc gcccgcgaca tcgtcatcga catgtggtgc taccgccgct
     1201 tcggccataa cgaaggcgac gagccgagct tcacccagcc gctcatgtat gccgagatcc
     1261 gcaagcaccc gccggtctcg gagctgtacg gcgcccggct ccgcgaggag ggcgtgatcg
     1321 atccggcctg ggccgaggct cgggtcggcg aattcaccgc tttgctcgag aacgagttcg
     1381 aggcggccaa atcctatctg cccaacaagg cggactggtt cgagggccgc tggtcgggcc
     1441 tcggtcggcc ggagaccccg gagacggagc gccgcaacgt ctccaccgga gtcgacgaaa
     1501 acacccttcg cgaagtgggg gcgatcctga cctcgatccc ggagggcctc accgtccaca
     1561 agaccctgca gcgcatcatc gacgccaagg ccgagatgtt cgccagcggc cggggcttcg
     1621 actgggcgac ggccgaggcg ctcgccttcg gcactctgct cagggacggc tgcggggtcc
     1681 gcctctccgg ccaggacagc gccaggggca cgttcagcca ccgccacgcc gcctgggtcg
     1741 atcagaccaa cgggcacaaa tatatcccgc tgacccgggt cgagggcgct cgattcgagg
     1801 tccgggattc gccgctctcc gagttcggag tgctcggcta cgaatatggc ttctcgatcg
     1861 ccgacccgca caccctggtg ctttgggagg cccagttcgg cgacttcgcc aacggcgccc
     1921 aggtcatcat cgaccagttc atcgccgccg gcgaggccaa gtggcttcga gcgagcgggc
     1981 tagtgatgct ccttccgcac ggctacgaag gccaggggcc cgagcacagc tcggcccgcc
     2041 cggagcgctt cctgcaattg tgcggtgagg acaatatgca ggtcgcctat tgcacgaccc
     2101 cggccaatta cttccacctg ctgcggcgcc agatgaagcg cgatttccgc aagccgctga
     2161 tcgtcatgac gcccaagtcg ctgctcaggc acaagctggc ggtctcgggc gtcgccgact
     2221 tcaccgggga gagccatttc aagcgcatcc tttccgacgt cggcggcccg gccgacgcgg
     2281 aggtcaagcg gctcgtcctg tgcagcggaa agatcgccta cgaactgatg gaatcgcgag
     2341 atcaggccga agatcgccac acttcgatcg ttcggctcga gcagctctat cccttcccgt
     2401 ccgagcccct gacgacccgc ctgaagcgca tgaccgctct cgaggaggtg gtctgggctc
     2461 aggaggagcc gcagaaccag ggctattgga atttcctcga ctggcggatc gaacgctgcc
     2521 tggaggaagc gggggtgaag ccgcggcggg cgctctatgc cggccgtccc gcctcggcgt
     2581 cgcccgccac cggcctcgcc aagcgccacg ccgcggagca ggcggccttg atcgccgacg
     2641 cgctcggcca tagcagtcac gcgccggcgc agcgcaaggc gggataaagg aacgacatgg
     2701 cgaccgaagt caaagtcccg accctgggcg aatcgatcac cgaggcgacc ctgggccaat
     2761 ggctcaagat gcccggcgat gcggtgaagg ccgacgagcc cgtcgccagc ctggagaccg
     2821 acaaggtcgc gatcgaggtg ccctcgccgg tcgacggggt gatgggcgac cagctcgtcg
     2881 cggtcggcga cacggtcgcg gtcggagccg ccatcgcccg gatcgagagc ggctccggca
     2941 tgcccgcgac cgctcagtcc gagcaggcga ccccggccgc cgtcccggtc aatccggaag
     3001 gcgcgggcga gaatgtcgag ctccgctccg acccggccga gtccgccgaa gacgatgacg
     3061 gccacaatct gaccttgtcg ccggccgtgc gtcggatcgt gctcgagcat cacctcgacc
     3121 catccaggat caagggcacc ggcaaggacg ggcggctgac caaggacgac gtcctggcgg
     3181 cggcggcaaa agcgaaggaa gcgcccgcgc ccgcgccggc agcgccacct ccgccgcctc
     3241 ccccggcggc cgccgctccc gcgcctcgtc ccccttcagg ggggcgccgg gaggaacggg
     3301 tgcggatgac ccgcctcagg cagaccgtcg cccggcgcct gaaggaggcg cagaacacgg
     3361 cggcgctgct caccaccttc aacgacgtcg acatgacggc ggtgatggag gcgaggagcc
     3421 ggtacaagga tctgttcgag aagaagcacg gaatccgcct gggcttcatg agcttcttcg
     3481 tgaaggcggc cgtgctcgcc gcccgcgacg tgccttcggt caacgcctac atagaaggcg
     3541 aggagatcgt ctatcacgat tatgtcgacg tctcggtcgc cgtctcggcg ccgaacggcc
     3601 tcgtcgtccc ggtcatccgc gacgcgcagg cgctgagctt cgccgaggtc gagaagacga
     3661 tcgccgagtt cggcaagagg gccaaggaag gctcgctgac catggccgac atggaaggcg
     3721 gcaccttcac catctccaac ggaggcgtgt tcggttcgct cctctccacc ccgatcatca
     3781 acccgccgca gtccgccgtg ctcgggatgc accggatcga ggagcggccg gtggtccgcg
     3841 acggccaggt ggtggttcgg ccgatgatgt atctggcgct gagctacgac caccggctgg
     3901 tggacggccg ggaggcggtc accttcctgg tccggatgaa ggaagctctg gaagacccga
     3961 cgcggctgtt gatcgatcta tgagcatgcg aggcctggag aacggcatca tagttatgct
     4021 cgtgctcttc ggcgcaatgt caatgctgtg gattgtggga catgctgatc aatggctgca
     4081 gaagctccgc agagctccag aggtgagtgg ccggcaccat gatggaccga ttgcaaaaat
     4141 tggaattgcg gtaattttgt tggctctact gggcctctgc ctctggacag gtgggggctc
     4201 gcaggccgtg gacgggtgct ttggtggcat gcggtgctga actcaggtcg aacggataaa
     4261 tgacgaaagc gctggatcgg acgccttggg acggcatcat tgtcaccgat gcagatacgt
     4321 gcttcggcaa agcgcggatt gcgggcacgc gcatttatgt cgactttatt ctgcaggcca
     4381 tcgaaggcgg atggtcgatc gacgattttg tacgtgagta tccacacgtg acgagggctc
     4441 aggttcaggc tgtaatcggc ttcgcgcgcg atcttgttgc agcaaaacgc aatcggttga
     4501 aaggcgagca gcgacgtgcg gctccggccg aggagataga gcaggcatga ctgataccca
     4561 gttcgacgtg atcatcatcg gcgctggccc gggcggctac gttgccgcga tccgcgccgc
     4621 ccagctcggg ctgaagacgg cgtgcgtcga gagccgtgag acgctcggcg ggacctgcct
     4681 caacgtcggg tgcatcccgt ccaaggccct tctccatgct tccgagctgt tcgaggaagc
     4741 ggcgcacggc accatggcga agtggggcat caaggcgacg ggggtcgagc tggatctcga
     4801 cgtcatgcat gccggccgca aggaggcgat caagggcctt accgggggca tcgagttcct
     4861 gttcaagaag aacaagatca cctggctcaa gggccacgcc aagttcacgg gtccgaacgc
     4921 gatcgatgtc gccggacagg cttattcggc gaagagcatc gtcatcgcca ccggctcctc
     4981 ggtgactccg ctgccggggg tcgagatcga ccaggagcgg atcgtcgatt ccaccggggc
     5041 cctggagctc gccagggtgc ccggacacat ggtcgtgatc ggcggcgggg tgatcgggct
     5101 ggagcttggc tcggtctggc ggcggctggg ggccagggtg acggtggtcg agtatctcga
     5161 ccagctgctg cccggaatgg acggcgaggt ccgcaaggaa gccggcaaga tcttcaagaa
     5221 gcaggggttc gagatccgga cctcgaccaa agtgacgaag gcggagaaga ccggggaggg
     5281 cgtggccctt accctcgagc cggcgcaggg cggcgaggcg cagacgctcg aggcggacgt
     5341 ggtgctggtc tcgatcggtc ggcggccgaa cgtcgacggg ctcaacttgg aagccgccgg
     5401 gatccagctc aacaggcgcg gccagatcga ggtcgaccac gaattcagga ccggggtgga
     5461 gggcgtctat gcgatcggcg acgtcactcc gggcccgatg ctcgcccaca aggccgagga
     5521 cgaggggatc gccgtcgccg aaatcctcgc cggccaggtc gggatcatca atcacgacgt
     5581 catcccgggg gtcgtctaca cctatccgga gatcgcctcg gtcggaaaga ccgaggagga
     5641 actcaaggag gcgggagtcg aatataagac cggcaagttc ccgatgatgg cgaacagccg
     5701 cgccaagacc aaccgcgata cggacggctt cgtcaaggtg atcgccgacg cccgcaccga
     5761 ccgggtgctg ggagtgcaca tcatcgcctc ggtcgccggg acgatgatcg cccaggccgc
     5821 ccaggcgatg gagttcggag cgaccagcga ggacatcgcc tacacctgcc acgcccaccc
     5881 gacccattcg gaagcgatga aggaagcggc gatggcggtg acggggctgc cgatccacat
     5941 ctgatctggg cttgccgctt gcagcttccg cgcagaggat tagcatcgcc ccaaacaggg
     6001 gaaatacatg cccgcgatcc tgattgcggc cg
//
LOCUS       DASWNU010000604         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_153856, whole genome shotgun sequence.
ACCESSION   DASWNU010000604 DASWNU010000000
VERSION     DASWNU010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153856"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGB57_12060"
     CDS             complement(<1..522)
                     /locus_tag="VGB57_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0132128.1"
                     /translation="MIQKVLIANRGEIALRIHRACHEMGIKTVAVHSTADTDAMHVRL
                     ADEAICIGPPAAADSYLNIPAIISAAEISHADAIHPGYGFLSENARFAEIVELHNIIW
                     IGPKPEHIRIMGDKVEAKRTAARLGLPLVPGSDGPVEGIEEAKKVAAETGYPVLIKAA
                     SGGGGRGMKVVRSE"
     gene            complement(641..1114)
                     /gene="accB"
                     /locus_tag="VGB57_12065"
     CDS             complement(641..1114)
                     /gene="accB"
                     /locus_tag="VGB57_12065"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY0132129.1"
                     /translation="MSDEERAESGMRIEADLVRQLAELLNQTDLTEIEVEDGDRKIVV
                     KRSILVAAAAPAPAAAPAAPAAAPAAAAPAVSPANHPGAVKSPMVGTVFLAGEPGARP
                     FVSPGQTVREGDTLLIIEAMKVMNPIVAPRAGTVSAVLVLDAQPVEYDQPLVILE"
     gene            complement(1169..1609)
                     /gene="aroQ"
                     /locus_tag="VGB57_12070"
     CDS             complement(1169..1609)
                     /gene="aroQ"
                     /locus_tag="VGB57_12070"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0132130.1"
                     /translation="MARPPIIYVLNGPSLNLLGTREPEIYGPDTLDEIAGRLEDRARA
                     LGVEVDIRQSNHEGHLIDWLHEAQAEGAKAVILNAAGFTHSSVALHDAVKAISTPVIE
                     VHLSNPQARESFRRRSLIAPAARGTIAGFGALSYLLALDAAARL"
     gene            1648..1857
                     /gene="thiS"
                     /locus_tag="VGB57_12075"
                     /pseudo
     CDS             1648..1857
                     /gene="thiS"
                     /locus_tag="VGB57_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
BASE COUNT          279 a          613 c          681 g          324 t
ORIGIN      
        1 ttcggaacga accactttca tgccgcgccc gccgccgccg gacgcggcct tgatcagcac
       61 cggatagccg gtttcggcgg caaccttttt agcctcctcg atgccctcga ctggcccgtc
      121 cgagcccggc accaggggaa gcccgaggcg cgctgcggtg cgcttcgcct cgaccttgtc
      181 gcccatgatc cggatatgct cgggcttggg cccgatccag atgatgttgt ggagctcgac
      241 gatctcggcg aagcgggcat tttcggacag gaagccgtag cccgggtgga tcgcgtcggc
      301 gtggctgatc tcggccgccg agatgatcgc gggaatgttg aggtaggaat ccgcggccgc
      361 ggggggccct atgcagatcg cctcgtcggc gaggcgcacg tgcatggcgt cggtatcggc
      421 ggtggaatgc acggcgaccg tcttgatgcc catttcatgg caggcgcggt ggatccgcaa
      481 agcgatctcg ccccgattgg cgatgaggac cttctggatc atcgaaccgt catcccggcg
      541 gaagccggga cctcgggccg gaagggcgct gcatatgcgc ctgcgcggat tctcgctggg
      601 atcccggttt tcgccgggac gacggggatg tgcgccatca ctactcgaga atcaccagcg
      661 gctggtcgta ctcgaccggc tgcgcgtcga ggacgagcac ggcgctcacg gtgccggcgc
      721 gcggggcgac gatcgggttc atcaccttca tcgcctcgat gatcagcagg gtgtcgcctt
      781 ccctgaccgt ctggccgggc gagacgaagg gcctggcgcc gggctcgccc gcgaggaaga
      841 cggtgccgac catgggcgat ttgacggcgc ccggatggtt ggcgggagaa acggccgggg
      901 cggcggcggc cggcgcggcg gccggcgcgg ccggagcggc cgcgggagcc ggcgcggccg
      961 cggccacgag gatgctgcgc ttgacgacga tcttgcggtc gccgtcctcg acttcgattt
     1021 cggtcagatc ggtctggttg aggagctcgg ccagctggcg cacgagatcg gcctcgatcc
     1081 gcatgccgct ctcggcgcgt tcttcatcgc tcatttcgaa ggtctctttc cctgttccgg
     1141 agccgtggcc ccgaagggcg gcttcctgtc agagacgggc cgccgcgtcc agagccagca
     1201 gataggagag ggcgccaaat ccggcgatcg tgcccctcgc ggcgggagcg atcaggctcc
     1261 tccggcggaa gctctcgcgc gcctgcgggt tggagaggtg gacttcgatc accggcgtcg
     1321 agatcgcctt caccgcatcg tgcagggcga ccgagctgtg cgtgaagccg gcggcgttga
     1381 ggatcacggc cttcgcgccc tcggcctgcg cctcgtgaag ccagtcgatc agatgcccct
     1441 catggttgga ctggcggata tccacctcca cgcccagcgc ccgcgcccgg tcctcgagcc
     1501 ggccggcaat ctcgtcgagg gtgtcgggcc cgtagatttc gggctccctc gtgccgagca
     1561 ggttgagact gggcccgttc agcacataga tgatgggtgg cctggccatg gcgagggcta
     1621 tagggggagg cggcaaggag cgcaaaggtg gacgagacgg cttcgatcac ggtcaacggc
     1681 gagcaccggc gggtgcccaa gggcatcagc gtcgccgatc tcgcgctcga gctggggctg
     1741 gagccgaccc gcgtggcggt cgagcgcaac ctcgagatcg tgcctcgctc cacgctcggc
     1801 caggtcatgg tcgaggacgg cgacgattac gagatcgtca ccttcgtcgg cggcggctga
     1861 agctatccgc ccttcaatcc tcccccggtt tttccgg
//
LOCUS       DASWNU010000605         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_153874, whole genome shotgun sequence.
ACCESSION   DASWNU010000605 DASWNU010000000
VERSION     DASWNU010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153874"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGB57_12080"
     CDS             complement(<1..218)
                     /locus_tag="VGB57_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0132131.1"
                     /translation="MFTASDSERASWRVSLLAELDSALARGELWNAYQPKLDLRGGVV
                     SGVEALVRWDHPGRGTLAPDSFIPLIEQ"
     gene            211..729
                     /locus_tag="VGB57_12085"
     CDS             211..729
                     /locus_tag="VGB57_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0132132.1"
                     /translation="MNISATLLHDHSFLHRLEQLLQASGFPADRVTLEVTESAAMADP
                     EAAGAALEGWRKLGVGVSIDDYGTGQSSLSYLQKMPATELKIDRSFIAAMAAGPRDRI
                     LVRSTIAMAHELGLAVVAEGVEDEICLELLREMRCDQAQGYGISRPLAADAVAEFARN
                     YACPAPPKRAAG"
     gene            complement(763..1404)
                     /locus_tag="VGB57_12090"
     CDS             complement(763..1404)
                     /locus_tag="VGB57_12090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0132133.1"
                     /translation="MRLPDDQRAAPVTLVIAVVTAAAWLIAWMGGWQEVAVMGGGFIP
                     ARIGGVPVGPEVVPVLLTPLTSALVHSGFAHLFFNLIILLFCGRSVERVVGGQGIVIL
                     YLVGAYAAAAAQYAVDPAEMAPMVGASGAISAILGAFALLFGRNKVKVAHPGLAALLH
                     ALWLAAAWIGLQILIGLTFETSGLRIAIAAHIGGFLLGLALARPLLLLRWRGA"
     gene            complement(1520..1999)
                     /gene="greA"
                     /locus_tag="VGB57_12095"
     CDS             complement(1520..1999)
                     /gene="greA"
                     /locus_tag="VGB57_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY0132134.1"
                     /translation="MATVEKNPMLAEGYEKLQTEVRHLKTVERPSVIEAIEIARGHGD
                     LSENAEYHAAKERQGQVEAQIADIEDRLSRAMVIDPTTLSGDKVVFGATVHLLDEDDK
                     PVTYQIVGQTEADARIGRISYNSPLGRALIGRTIGEEVEVTTPSGDRYYSIEKVEFI"
     gene            complement(2084..>2767)
                     /locus_tag="VGB57_12100"
     CDS             complement(2084..>2767)
                     /locus_tag="VGB57_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY0132135.1"
                     /translation="TVPFVAKTLGTPIAKIAARVMAGESLMGFLPLRTDIGHIAVKEA
                     VFPFARFPGIDPVLSPEMKSTGEVMGIDDDFAKAFLKSQLGSGIQLPLAGTLFVSVKD
                     SDKSAITAPVRALRELGFKVIATAGTASWLGGEGIEVSQVNKVAQGRPHIVDRIKDGA
                     VDIIFNTTEGWQSHKDSASIRASALMAKVPYFTTAASSAAVVRAIAALGEHSLEVKSL
                     QDYYSSSKA"
BASE COUNT          462 a          929 c          921 g          455 t
ORIGIN      
        1 tgctgctcga tcagcgggat gaagctgtct ggggccaggg tgcctcgccc cggatggtcc
       61 cagcggacga gcgcctcgac tccgctgacc actccgccgc gcaggtcgag cttgggctgg
      121 taagcattcc agagctcgcc ccgcgcgagg gcggaatcga gctcggcgag cagtgacacc
      181 cgccagctcg cccgttcgga atcgctggcg gtgaacatct cggccaccct gctgcacgat
      241 cattccttcc tgcaccgtct cgaacaattg ctccaggcca gcggcttccc cgcggatcgg
      301 gtcacgctcg aggtcaccga gagcgccgcc atggcggatc cggaggcggc gggggcggcg
      361 ttggaaggct ggcgcaagct tggagtcggg gtctcgatcg acgattacgg caccggccag
      421 tcgtcgcttt cctacctcca gaaaatgccc gcgaccgagc tcaagatcga ccggagcttc
      481 atcgccgcca tggccgcggg cccgcgcgac cggatcctgg tgcgctccac catcgccatg
      541 gcccacgagc tcggcctcgc cgtggtagcc gagggcgtcg aggacgagat ctgcctcgaa
      601 ttgcttcgcg agatgcgctg cgaccaggcc cagggctacg gcatcagccg gcctttggcg
      661 gcggacgcgg tggccgagtt cgcccgcaac tatgcctgcc cggcgccgcc gaagcgggcc
      721 gccggctgaa aatcctcccc atgctgcgcg tggggaggat aactacgccc cgcgccacct
      781 cagcaagagc agcggccggg ccagagcgag gccgaggagg aagccgccga tatgggcggc
      841 gattgcgatc ctgaggccgc tcgtctcgaa ggtcagcccg atcagtatct gcaacccgat
      901 ccaggccgcg gcgagccaca aagcgtggag caaggcggcg aggccggggt gagcgacctt
      961 caccttgttg cgcccgaaga gcaaggcgaa ggcgccgagt atggcggaga tggcgccgct
     1021 cgctccgacc atcggcgcca tctcggcggg gtccaccgca tattgagcgg ccgcggccgc
     1081 ataggcgccg accaggtaga ggatgacgat cccctgcccg cccaccaccc gctccaccga
     1141 gcgtccgcag aagagcagga tgatcaggtt gaagaacagg tgcgcgaaac cggaatggac
     1201 gagcgccgag gtgagtggcg tgagcagcac cggcaccact tccggaccga ccggcacccc
     1261 tccgatccgg gccggaatga agccgccgcc catgacggcg acctcctgcc atccgcccat
     1321 ccaggcgatc agccaggcgg cggcggtcac aaccgcgatc accagggtga ccggcgcggc
     1381 gcgctggtcg tcgggcaggc gcatcagccc cgacccgtca ttgcgaggag cgacgcgacg
     1441 acgcaatcca gcgggcctcc gacgctgcac tggattgctt cgcttcgctc gcaatgacgg
     1501 tttgcggggc gctcatgcct cagatgaact cgaccttctc gatcgaataa taacggtcgc
     1561 cggacggggt ggtgacctcc acctcctcgc cgatcgtccg gccgatgagc gctcggccga
     1621 gcggcgaatt gtacgagatc cggccgatcc gggcgtccgc ctcggtctgg ccgacgatct
     1681 ggtaggtgac cggcttgtcg tcctcgtcca gcaggtggac ggtggctccg aacacgacct
     1741 tgtcgcccga aagggtcgtc ggatcgatca ccatcgcccg gctcagccgg tcctcgatgt
     1801 cggcgatctg cgcctcgacc tggccctggc gctccttggc ggcgtgatat tcggcattct
     1861 cggaaagatc gccatggccg cgggcgatct cgatcgcctc gatcaccgag ggacgctcga
     1921 ccgtcttgag gtgccgcact tcggtctgga gcttctcata gccctcggcc agcatcgggt
     1981 tcttttcgac cgtcgccatc gccactcacc tttcgtcaaa aacctatttc gggcgccttc
     2041 ccgggtggga ggacgccctg attgcgtgtt cgattgttca ggatcacgct ttggaggatg
     2101 aataatagtc ctgcagggac ttcacttcaa ggctgtgctc cccgagcgcc gcgatcgccc
     2161 ggaccaccgc cgcgctggag gcggccgtgg tgaagtaggg aaccttcgcc atcagcgccg
     2221 aagcccggat cgaggcggaa tccttgtggc tctgccagcc ttccgtcgtg ttgaagatga
     2281 tgtcgacggc gccgtccttg atccggtcga cgatatgggg gcggccctgg gccaccttgt
     2341 tgacctggct gacctcgatc ccctcgccgc cgagccagga ggcggtgccg gcggtggcga
     2401 tgaccttgaa gccgagctcg cgcagcgccc tcaccggcgc cgtgatcgcg ctcttgtcgc
     2461 tgtccttgac cgacacgaac agggttccgg cgagcggcaa ttgtatgccg ctgccgagct
     2521 gggacttgag gaacgccttg gcgaaatcat cgtcgattcc catgacttcg ccggtggact
     2581 tcatttccgg cgacaggacg ggatcgatgc cggggaagcg cgcgaacggg aagaccgcct
     2641 ccttgacggc gatatggccg atatcggttc gaagcggcag gaagcccatg agcgattccc
     2701 cggccatcac ccgggcggcg atcttggcga tcggggtccc aagcgtcttg gcgacgaagg
     2761 ggacggt
//
LOCUS       DASWNU010000606         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_177838, whole genome shotgun sequence.
ACCESSION   DASWNU010000606 DASWNU010000000
VERSION     DASWNU010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177838"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VGB57_12105"
     CDS             <1..1441
                     /locus_tag="VGB57_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEY0132136.1"
                     /translation="DNATRRAEQTLARLDIKAQRINGLLARSASTIRAASAAVSLAPA
                     DSPVCANTLQRLARAQGTPGRFALYAAGNSLRCATPGYAPPANLPNPGRRHSTIQITP
                     DGELLRFALYDDQGQLEGIGEFGRAGLAELTLLPGTTPDFDLELWQGEHRMMLRNGYT
                     PGTLTRAVSSTVPVAGDRLKLRIMLAATPVSAAEMLTILLPLLMLIWAGIVSWMVLNR
                     LLLRPLGRMERAVSAYQPGDAHLDLPKLTTPAREIGDLGAAFDRVTQTVARHEADLGA
                     AVDRQTRLVREVHHRVKNNLQVVASLLNLHARGAANEEVAAAYASIQRRVDALAVVHR
                     NHFAELEENRGVALRSLISELGANLRATAPASASNMQIRLAIEPYHVTQDVAVSVAFL
                     VTEIVEYAMFCDARSVAVSVSGDSGATARLAIESDSLRGETRCDQNLTDRFERIVTGL
                     SRQLRSSLEKDPETGRYALNIAVLDKSEV"
BASE COUNT          238 a          597 c          491 g          261 t
ORIGIN      
        1 cgacaacgcc acccggcggg ccgagcagac tttggcgcgg ctcgatataa aggcgcagcg
       61 catcaacggc ctcctcgccc gcagcgccag cacgatccgc gccgcgagcg ccgcggtgtc
      121 gctggccccg gccgactcgc ccgtctgcgc gaacacgctc cagcgcctcg ctcgggcgca
      181 gggcacgccg ggccgcttcg ccctctatgc cgccggcaac agcctccgct gcgccacccc
      241 cggctacgcc ccgcccgcca atctgccgaa cccgggccgc cggcactcga cgatccagat
      301 cacgccggac ggcgaattgc ttcgctttgc cttgtacgac gatcaggggc agctcgaagg
      361 aatcggcgaa ttcggccggg ccggtctggc cgaattgacc ctcctccccg gcaccacgcc
      421 cgatttcgac ctcgagcttt ggcagggcga gcaccggatg atgctgcgca acggctatac
      481 gccggggacg ctgaccaggg ccgtgtcctc gactgtgccg gtcgccggcg atcggctgaa
      541 gctcaggatc atgctcgcgg cgaccccggt cagcgcggcc gagatgctca ccatcctgtt
      601 gccgctgctg atgctgatct gggctggaat cgtcagctgg atggtgctca accgcctgct
      661 gctgcgcccg ctcggccgga tggaacgcgc cgtttccgcc tatcagccgg gcgacgctca
      721 tctggacctg cccaagctca cgaccccggc gcgcgagatc ggcgacctcg gcgccgcctt
      781 cgaccgggtc acccagaccg tggctcgcca cgaggccgat ctcggggcgg ccgtcgatcg
      841 ccagacccgg ctggtgcgcg aagtccatca ccgggtgaag aacaacctcc aggtcgtcgc
      901 ctctttgctc aacctgcacg cccgcggcgc cgccaacgag gaggtcgccg ccgcctatgc
      961 ctcgatccag cggcgggtcg acgccctcgc cgtcgtccac cgcaaccatt tcgccgagct
     1021 ggaagagaat cgcggggtcg cgctgcgatc gctcatttca gagctggggg cgaaccttcg
     1081 cgccaccgcc cccgcgtccg cctcgaacat gcagatcagg ctggccatcg aaccttatca
     1141 cgtcacccaa gacgttgccg tctccgtagc cttcctcgtc accgagattg tggaatatgc
     1201 gatgttctgc gacgcccgtt ctgttgccgt ctcggtcagc ggcgacagtg gcgccaccgc
     1261 tcggctggcg atcgaatcgg acagccttcg cggtgaaacc cgttgcgacc agaacctgac
     1321 ggaccggttc gaacggatcg tcaccggcct ctcccgccag ctccgctcct cgctggagaa
     1381 ggaccccgag acgggccgct acgccctcaa catcgccgtc ctcgacaaga gcgaggtctg
     1441 atcgagcccg ggcggcagcc ttgggctagt cgctccgatc gtcgctggcg ggcttgtcat
     1501 cgctgtaggg gggccttgcg accgtctccc ggctcgcctc cgccaggtcc ttattgtcct
     1561 gccctgcctc attggcggta tcgacgt
//
LOCUS       DASWNU010000607         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_205348, whole genome shotgun sequence.
ACCESSION   DASWNU010000607 DASWNU010000000
VERSION     DASWNU010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205348"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /gene="hutU"
                     /locus_tag="VGB57_12110"
     CDS             complement(<1..1529)
                     /gene="hutU"
                     /locus_tag="VGB57_12110"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY0132137.1"
                     /translation="MSNGRHIESPRGPEISSRSWLTEAAMRMLMNNLDPRVAEAPEEL
                     VVYGGIGRAARDWESFDTLVETLRRLGEDETLLVQSGKPVGVFRTHADAPRVLIANSN
                     LVPKWADWDHFNELDRKGLMMYGQMTAGSWIYIGTQGIVQGTYETFVEMGRQHYGGDL
                     SGRWILTAGLGGMGGAQPLAAVMAGAHCLAIECQPSRIEKRLETKYLDQATDDLDEAL
                     RILSAATSPTSVGLLGNAAEILPEMVRRGIRPDAVTDQTSAHDPVNGYLPAGWTVEEW
                     IEKRERDPGAVEAAARKSMAVHVEAMLAFRAMGVPTFDYGNNIRQVAKDEGVERAFDF
                     PGFVPAYVRPLFCRGIGPFRWCALSGDPEDIYRTDARVKELIPDDPHLHRWLDMARER
                     IAFQGLPARICWVGLGQRHRLGLAFNEMVASGELEAPVVIGRDHLDSGSVASPNRETE
                     AMRDGSDAVSDWPLLNALLNTASGASWVSIHHGGGVGIGRSIHSGQVSVADGTPLAAE
                     KL"
BASE COUNT          258 a          538 c          550 g          255 t
ORIGIN      
        1 agcttctcgg cggcgagcgg cgtgccgtcg gcgaccgaca cctgtccgga gtggatggac
       61 cggccgatgc cgaccccgcc gccgtggtgg atcgagaccc aggacgcgcc ggacgcggtg
      121 ttgagcaggg cattgagcag cggccagtcg gagaccgcgt ccgagccgtc cctcatcgcc
      181 tccgtctccc gattcggcga ggcgaccgag cccgaatcga gatggtcgcg gccgatcacg
      241 accggggcct cgagctcgcc cgacgccacc atctcgttga acgccaggcc gagacggtgg
      301 cgctgcccga gaccgaccca gcagatccgc gccggcaggc cctggaaggc gatccgctcc
      361 cgcgccatgt cgagccagcg gtggaggtgg ggatcgtcgg ggatcagctc tttgaccctc
      421 gcgtcggtgc ggtagatgtc ctcggggtcg cccgagagcg cgcaccagcg gaagggaccg
      481 atgccgcggc agaagagcgg gcggacatag gccgggacga agccggggaa atcgaaggcg
      541 cgttcgacgc cctcgtcctt cgcgacctgg cggatgttgt tgccatagtc gaaggtcggc
      601 acgcccatgg cgcggaaggc gagcatcgcc tcgacatgga cggccatcga cttgcgggcg
      661 gcggcctcga ccgcgccggg gtcccgctcg cgcttctcga tccattcctc gaccgtccag
      721 ccggcgggca agtagccgtt gacggggtcg tgggccgagg tctggtcggt gaccgcatcg
      781 ggacggatgc cgcggcggac catctcgggg aggatctcgg cggcgttgcc gagcaggccg
      841 accgatgttg gcgaagttgc cgcggagagt atcctcaacg cctcgtccag atcgtctgtt
      901 gcttggtcta agtacttggt ttccaagcgt ttttctatgc ggctcggctg gcactcaatg
      961 gcgaggcagt gggcgcccgc catcaccgcc gcgagcggct gagcgccgcc catgccgccg
     1021 aggcccgcgg tcaggatcca gcggccggaa aggtcgccgc cataatgctg gcggcccatc
     1081 tcgacgaagg tctcgtaggt gccctggacg atgccctgag tgccgatata gatccacgag
     1141 ccggccgtca tctggccgta catcatcagc cccttgcgat ccaattcgtt gaaatggtcc
     1201 cagtccgccc atttcggcac gaggttcgaa ttggcgatca gcacgcgcgg cgcatcggca
     1261 tgggtgcgaa acacgccgac cggcttgccg gactggacca gcagggtttc gtcctcgcca
     1321 agccggcgca gcgtctcgac gagggtgtcg aagctctccc agtcccgcgc cgcgcggccg
     1381 atgccgccat agacgaccag ctcctcaggc gcctcggcga cgcgcggatc gagattgttc
     1441 atcagcatcc gcatcgccgc ctcggtcaac cagctccggg agctgatctc cggccccctc
     1501 ggcgactcaa tgtggcggcc attcgaaatc ggacgcgacc tttggagagc aggggcggtg
     1561 ttcacgcggc aagccccggg agttcgctaa ccatatccat c
//
LOCUS       DASWNU010000608        10259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_333326, whole genome shotgun sequence.
ACCESSION   DASWNU010000608 DASWNU010000000
VERSION     DASWNU010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333326"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VGB57_12115"
     CDS             complement(<1..776)
                     /locus_tag="VGB57_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY0132138.1"
                     /translation="MALLDSIYFKALGEALRWRGREIGCGAALLVTASCVPLKAADAP
                     VDAATPANVATLAPAPQPEPMVLRDVAPTEASRINASIPFSADANPRAPSTVFRAASP
                     GDQFRSLQCLAEAIYYEARSESEDGQRAVAQVVLNRVRHPAWPGTVCGVVYQGPLRAG
                     GGCQFTFTCDGSLLRMPYGVDWARARRLAAEALAGHVFAPVGLATNYHTHQVLPDWAF
                     RLPKATVIGNHIFYRLPGAWGGAPAFSQAYRGREPSPATV"
     gene            983..2578
                     /locus_tag="VGB57_12120"
     CDS             983..2578
                     /locus_tag="VGB57_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0132139.1"
                     /translation="MRKILVCVSLAALLAGGCDFVSTSGGGGGSSGQVVQVDPAAVTP
                     ADLQAAVTDPRVKKFYEARGWKAVWTGERAQELAAAFEDAARHAIDAKSYAEKATKGA
                     SPAEREAGLTLAAMDYAQALATGAVDPRKLFEVYTVPMSRVDVGGGLGKAVEQGGVRQ
                     WLASLAPQDAEYKALSDAYLAYRQKAGQEQRAAVAPGEQIKPGKRDPRVAQIREGLRA
                     NGYLAAEPAAPATGDAKAKEGKARAAAPAAGGDVYGADMVAAVKKVQADYGIEADGVV
                     GNSTIEALNTGAKDRARILAVNLERRRWLDRAPPATRIDVNTAAAELHYYKDGAEKDR
                     RRVVVGQPDWETPELGSPIARLVANPPWTVPESIAKEEILPKGAAYMAKENIVMKNGK
                     LVQQPGPKSALGLVKFDMINNEQIYLHDTPAKALFASTQRHASHGCSRVENAVEFARL
                     IAQDDGKLEAFDKAMATKEETPVELSNKIPVRLLYHSAYVDGGRVVFRTDPYAWDDKV
                     AAALGLPGQIRPRVKTHVADIGP"
     gene            complement(2716..2880)
                     /locus_tag="VGB57_12125"
     CDS             complement(2716..2880)
                     /locus_tag="VGB57_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132140.1"
                     /translation="MRSNSGGGKSSNNTGNPTLEEHESAKGRSGAAIRGEKKIGKSPD
                     RTTPRDKQSS"
     gene            3143..3475
                     /locus_tag="VGB57_12130"
     CDS             3143..3475
                     /locus_tag="VGB57_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY0132141.1"
                     /translation="MHRTLSALAALAMTTGTLVLAVPAHAAPAEDVFSIDLDGLDSSD
                     PADALRIDRRIRTAAQSLCGSKLIQPTRMRGRAVACEQAVTADARSAVQLAAAKQGAP
                     LRLALRVR"
     gene            complement(3521..4267)
                     /locus_tag="VGB57_12135"
     CDS             complement(3521..4267)
                     /locus_tag="VGB57_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0132142.1"
                     /translation="MPDTIRFRVVDDEPLAVERLQLLLARMEGVTLAGTASEGAAALR
                     MVEATRPDAVLLDIAMPGMDGIDVARALARVPKPPVVIFVTAFDMFAVAAFDVEAVDY
                     VMKPVSAERLGRAVERARERIAGGGASPAGAGSGSPFVEEFWVSEARGLVRLSAADVD
                     RVSAERDYMRLHVGKRSWLINHTIGKLEEELDPARFVRLHRSAIVRRDFVTGLRRDEG
                     GRWWARLADESEQKVGRLYLANAKGLAGRR"
     gene            complement(4260..5465)
                     /locus_tag="VGB57_12140"
     CDS             complement(4260..5465)
                     /locus_tag="VGB57_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0132143.1"
                     /translation="MESFTDYRAHRRLPDLPLAAASILGFWLFYFATIVIRTWLIDES
                     LVWLGSRALGCLIGIVLTFLVYLVLSRAARESVRIQIVAAAIACVPAAAIFSTFNLAF
                     AVHQPLADRTVVGQRADGATIVRSTRGTEVIIGKGGSPVVLPGSPNRQSERQRQLMLR
                     LIADGLVTWYFFFAAWASFYIAISSAARLRTAERRAAGAERDAQEAQLRALRYQVNPH
                     FLFNTLNSLSSLVMARRDQEAEAMIVNLSAFFRSSLALDPSDDITLAREIEFQQLYLD
                     IEKARFPKRLDVRIEVPADLRGARVPPLILQPLVENAIKHGVARTAEPVALTIAAREE
                     DARLILIVENDRGPAEPAKSEHCTGVGLVNVCERLAARFGAEAECEHGPLPDGGWRVT
                     LARPLEIHA"
     gene            5908..6456
                     /locus_tag="VGB57_12145"
     CDS             5908..6456
                     /locus_tag="VGB57_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132144.1"
                     /translation="MATNTKPADGALTLVEMKEFAGFPAATQRYIRRSLDVGLGRRDA
                     LRRWSRDVGEAASIRAQERMYRRLDHVRASVPDDSGLDEMEPVMGPLVTMTAFDLGQD
                     RLPDFASYRFLYERLIGAAVRPWLPGAFCAAAALPHLHPEKRRALLQSISEAAATAPG
                     WSNREPVFFPEWVDKVDVTVAG"
     gene            complement(6540..7472)
                     /locus_tag="VGB57_12150"
     CDS             complement(6540..7472)
                     /locus_tag="VGB57_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0132145.1"
                     /translation="MPRKAHKIRTLAVTGGTGFVGSHLLRMALGEGYDVRALTRGWKP
                     PEDEIAWVDGALDRPETLLKLCIGADAVIHVAGAINAQGRAGFEAVNVAGTAAMIDAA
                     RKAGVRRFVHISSLAAREPQLSAYGWSKARSERLVAASGLDWTIVRPPAIYGPGDRET
                     LDLFKMARRGLVALPPGGRFSVIHVEDLCRLILASLDDADSWAETYEPDDGREGGWQH
                     RHFARTLGRIYGRRAMTLAMPGPVLRIASGLDRLFRRRNAKLTRDRVGYFCHPDWVAA
                     AEKRPPERLWRPQVKTPTGLKETADWYREQGWLK"
     gene            complement(7609..8646)
                     /gene="obgE"
                     /locus_tag="VGB57_12155"
     CDS             complement(7609..8646)
                     /gene="obgE"
                     /locus_tag="VGB57_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0132146.1"
                     /translation="MHFLDQAKIFISSGAGGPGAVGFRREKYIEYGGPDGGDGGKGGD
                     IVFEAVDGLNTLIDFRYTQHFRAPRGKGGAGQNRTGAGGDDLLIKVPVGTQILSEDKE
                     HVLADFTRVGQRQVFLKGGDGGRGNASYKTATNRAPRQHGDGWPGEEMWVWLRLKLLA
                     DVGLVGLPNAGKSTFLNAVTNAQAKVGAYPFTTLHPKLGVVRHREREFVVADIPGLIE
                     GAAEGAGIGDRFLGHIERCRVLLHLVDSEVEDVAETYRIVREELEAYGAGLAEKPEVI
                     ALNKSDTLDDELIAALSDELEAEAGARPLPLSGVTGAGVEAVLDALLSHIIAAKAADT
                     DVEEPGSWSPL"
     gene            complement(8703..9281)
                     /locus_tag="VGB57_12160"
     CDS             complement(8703..9281)
                     /locus_tag="VGB57_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0132147.1"
                     /translation="MSIGRERLSPEESRAAALEAARALLIESGPQAVTLKAVGARIGK
                     THGNLLHHFGSAAGLQGELARLIGERVTAEIARVVERERQGEADPREIVDRTFDAFDR
                     EGAGALAAWMILSGNRDALDPILDAIHRLVDQLGAGHEDRPVHETTLWLVLAALGDSL
                     MGGPMASALGLPREQAREIATRHLVQLAALRS"
     gene            9326..10165
                     /locus_tag="VGB57_12165"
     CDS             9326..10165
                     /locus_tag="VGB57_12165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0132148.1"
                     /translation="MGREVTPSDLTITPRDRRFGRGATTERWWLGGNPYGSALYDALS
                     VTFPKGEAFFVESVRAFRDDAPPRLAEEIRAFTTQEVMHSREHVAFNRRAVEAGYDIA
                     PLEARVDYRIEETKSRPPIVNLAATMALEHFTAILANQLLGNPRHLKGADPQSAALWR
                     WHSMEEIEHKGVAYDTWLHATRNWPRRKRWMVKSLVMLKVTKNFFVERTQGALMLLEQ
                     DGIRGPRAWAGLFWYAMVSPGMFRKILVGWLKFFLPGFHPWKHDDRKLIAEGERSLEA
                     AAA"
BASE COUNT         1622 a         3438 c         3615 g         1584 t
ORIGIN      
        1 atcaccgtcg ccggagaagg ctcgcggccg cgataggcct ggctgaaggc gggggcgccg
       61 ccccaggcgc cgggcaggcg atagaagatg tggttgccga tcaccgtcgc cttgggaagc
      121 cggaaggccc agtccggaag cacctggtgg gtgtggtagt tggtggcgag cccgacgggc
      181 gcgaagacat ggccggccag cgcctcggcg gcgaggcgcc gggctcgggc ccagtccacg
      241 ccgtagggca tgcgcagcag cgagccgtcg caggtgaagg tgaactggca gccgccgccg
      301 gcgcggagcg gcccctgata gacgactccg cagacggtgc ctggccaggc ggggtggcgg
      361 acccggttga ggacgacctg ggcgacggcg cgctggccgt cctcgctctc ggagcgagcc
      421 tcgtaataga tggcctcggc gaggcattgg agcgagcgga actggtcgcc gggactggcg
      481 gcgcggaaga ccgtcgaagg cgcccgggga ttggcgtcgg ccgagaaggg gatcgaggcg
      541 ttgatccggg aagcttcggt cggagcgacg tcgcgaagga ccatcggctc gggctgcggc
      601 gccggagcaa gcgtggcgac attcgccggt gtcgcggcat cgacgggagc gtcggcggcc
      661 ttgagcggaa cgcaggaagc ggtgacgagc agggccgcgc cgcagccgat ctcccggccg
      721 cgccagcgca acgcctctcc caaagccttg aaatatatgg aatccagcaa agccaagttg
      781 gtcctcgtcg cctgttcgag tgcccgccta gcttccgata gctaataatg ttgcaccatt
      841 gcgccccccc atttcccgct gtcccagagc gggacgccgg cacggtgccg gttaagcggc
      901 tggggggctt atttttcttc ggagcctatt ggccggtggg gacgttagat ggacctgatc
      961 catgtgaagc ggcggtgtgt agatgcgtaa gattctggtg tgcgtttcgc tggcggccct
     1021 cctggccggc ggctgcgatt tcgtaagcac gagcggcggc ggcggcggta gctccggcca
     1081 ggtcgtgcag gtcgatcccg cggcggtgac tccggccgat ctccaggcgg ccgtcaccga
     1141 cccgcgggtg aagaaattct acgaagcccg cggctggaag gcggtctgga ccggcgagcg
     1201 ggcgcaggag ctcgccgcgg cgttcgagga tgccgcccgt cacgcgatcg acgccaaatc
     1261 ctatgccgag aaagcgacca agggcgcgag cccggccgag cgggaggcgg ggctgaccct
     1321 ggccgcgatg gactatgccc aggcgctggc cacgggggcg gtcgacccgc gcaagttgtt
     1381 cgaggtctat accgtgccga tgtccagggt cgacgtgggc ggagggctcg gcaaggcggt
     1441 ggagcagggc ggggtgcggc aatggctcgc cagcctcgct ccgcaggatg cggaatataa
     1501 ggcgctgtcc gacgcctatc tcgcctatcg ccagaaggcc ggccaggagc agagggcggc
     1561 ggtggccccg ggcgagcaga tcaaacccgg caagcgcgat ccgcgagtgg cccagatccg
     1621 cgaggggctg cgggccaacg gttacctcgc ggccgagccc gccgcgccgg ccaccggcga
     1681 cgccaaggcg aaggaaggca aggccagggc cgcggcgccc gccgccggcg gcgacgtcta
     1741 cggcgccgac atggtcgccg ccgtgaagaa ggtgcaggcc gattacggga tcgaggccga
     1801 cggagtggtc ggcaattcga cgatcgaggc gctgaacacc ggcgccaagg accgggcccg
     1861 gatcctggcg gtcaacctcg agcggcggcg ctggctggat cgggcgccgc cggcgacccg
     1921 gatcgacgtc aacacggcgg cggccgagct ccattactac aaggacggcg ccgagaagga
     1981 ccggcgccgg gtcgtcgtcg gccagccgga ttgggagacg cccgagctcg gttcgccgat
     2041 cgcccgcctc gtcgccaacc ccccgtggac cgttccggag tcgatcgcca aggaggagat
     2101 cctgcccaag ggtgccgcct acatggctaa agagaatatc gtcatgaaga acggtaagct
     2161 ggtgcagcag ccggggccga aatcggcgct cggcctcgtc aagttcgaca tgatcaacaa
     2221 cgagcagatc taccttcacg acactcctgc caaggcgttg ttcgcctcga cccagcgcca
     2281 cgccagccac ggctgttcgc gagtcgagaa tgccgtcgag ttcgcccgcc tgatcgccca
     2341 ggacgacggc aagctcgaag ccttcgacaa ggcgatggcg acgaaggagg agacgcccgt
     2401 cgagctttcg aacaagatcc cggttcggct gctctaccac agcgcctatg tcgacggcgg
     2461 gcgggtcgtc ttccgaaccg atccctatgc gtgggacgac aaggtcgccg ccgcactcgg
     2521 cctccccggc cagatcaggc cgcgggtcaa gacccacgtc gcggatatcg ggccgtaata
     2581 atcctcccga tcgaagatgg ggagggggac cccgcgtagc ggggtggagg ggtaattggc
     2641 agaagcctgt gcgattaccc ctccaccatg cttcgcatgg tccccctccc caagctgcgc
     2701 ttggggagga tcaggctagc tcgactgctt gtcgcgcggg gtggtgcggt cgggcgactt
     2761 gccgatcttc ttctcgccgc ggatggccgc gccggagcgg cccttggcgc tctcatgctc
     2821 ctccagcgtc ggattgccgg tgttgttgct gcttttgccg ccgccactgt tgctgcgcat
     2881 cgaatctctc ctttttcgtc tcgcgcacaa cgaggcagcg cccaattctt ctccgccggg
     2941 cgtgcgtttc gtcggccgaa ggatgcattt cgtcggcgcg cgggcggttc gcagagccgt
     3001 tgcgggcccg tgtcgaagcg aacgggcgcg gcggagcgga gcggtctagg tcgaatccag
     3061 cgacgcgggg ctccaacccc gtctcatccc cggggaaggg acctcgcgcc gcaccggatt
     3121 ttcgaagaca aggacgaaga ccatgcaccg tacgctttcc gccctcgccg ccctcgccat
     3181 gacgacgggc acgctcgtcc tcgccgtgcc ggcgcacgcc gctcccgccg aggatgtctt
     3241 cagcatcgac ctcgacggtc tcgactcctc cgacccggcc gacgccctcc ggatcgaccg
     3301 ccgcatccgc accgccgcgc agagcctttg cggctccaag ctgatccagc cgacccggat
     3361 gcggggacgg gccgtggcct gcgaacaggc cgtcaccgcc gacgcccgaa gcgccgtcca
     3421 gctcgccgcc gcgaagcagg gcgcgccgct ccgcctggcc ctgcgcgtcc ggtaagagga
     3481 agccaccgga cgacctggct cgccgggctc ccgcgacccc ttagcggcgc ccggcgagcc
     3541 cttttgcatt ggcgaggtag agccgcccga ccttctgctc gctttcgtcg gcgaggcgcg
     3601 cccaccagcg accgccctcg tcgcgcctaa gcccggtaac gaaatcgcgg cggacgatcg
     3661 cggaccggtg aagccggacg aacctcgccg ggtcgagctc ctcctccagc ttgccgatcg
     3721 tgtggttgat cagccagctc ctcttgccga cgtggagccg catatagtcg cgctcggcgc
     3781 tgacccggtc gacgtccgcc gcggacaggc ggaccaggcc gcgggcctcc gacacccaga
     3841 attcctcgac gaaaggcgaa ccggacccgg ccccggccgg gctcgcgccc ccgcccgcaa
     3901 tccgctcccg cgcgcgttcg accgcccggc cgagacgctc cgcggagacc ggcttcatca
     3961 catagtcgac cgcctcgacg tcgaaggcgg cgacggcgaa catgtcgaac gcggtgacga
     4021 agatgacgac gggcggcttc ggcacccgcg ccagcgcccg ggcgacgtcg ataccgtcca
     4081 ttcccggcat cgcgatgtcg agcagaaccg cgtcgggccg ggtcgcctcg accatgcgca
     4141 gggcggcggc cccttcggac gccgtaccgg cgagggtcac cccctccatc cgggcgagca
     4201 gaagctgcag gcgctcgacg gcgagcggct cgtcgtcgac gacgcggaag cggatcgtat
     4261 caggcatgga tctccagggg cctcgccagg gtgacgcgcc agccgccgtc gggcagcggg
     4321 ccatgttcgc attccgcttc ggcgccgaag cgggcggcga gccgttcgca gacgttgacc
     4381 aggccgactc cggtgcaatg ctcgctcttt gccggctcgg ccgggcctcg gtcgttctcc
     4441 acgatgagga tgagccgggc gtcctcctcg cgcgcggcga tcgtcagcgc aaccggctcg
     4501 gcggtgcggg caaccccgtg cttgatcgca ttctcgacca gcggctgaag gataagcggc
     4561 ggcacccgcg cgccgcgaag gtcggcgggc acttcgatcc ggacgtcgag ccgtttcggg
     4621 aaacgcgctt tctcgatgtc gagatagagc tgctggaact cgatctcccg ggcgagggtg
     4681 atgtcgtcgc tcggatccag cgccaggctc gagcggaaga aagcggaaag gttgacgatc
     4741 atcgcctcgg cctcctggtc gcgccgcgcc atgacgagcg aggacagcga gttcaaggtg
     4801 ttgaacagga agtgcggatt gacttggtag cgcagggcgc ggagctgcgc ctcctgggcg
     4861 tcgcgctcgg cgccggcggc gcggcgctcg gcggttcgaa ggcgggccgc cgagctgatc
     4921 gcgatgtaga agctcgccca ggctgcgaag aagaaatacc aggtgacgag accgtcggcg
     4981 atcagccgaa gcatgagctg gcgctggcgc tcgctctggc ggttggggga tccgggcagg
     5041 accaccgggc tcccgccctt gccgatgatc acctcggtgc cccgggtgga gcgaacgatc
     5101 gtggccccgt ccgccctctg gccgacgacg gtccggtcgg ccaaaggctg gtggacggcg
     5161 aaggccagat tgaaggtcga gaagatcgcc gcggcgggaa cgcaggcgat ggcggccgcg
     5221 acgatctgga tcctgacgct ctcgcgcgcg gctctgctca gaacgaggta gacgaggaag
     5281 gtgaggacga ttccgatcag gcagcccagg gcgcgcgagc cgagccagac gaggctctcg
     5341 tcgatcagcc acgtgcggat gacgatggtc gcgaaataga acagccagaa gccgaggatc
     5401 gaggccgccg cgagcggcag atcgggcagg cgccgatgcg cgcgatagtc ggtgaagctc
     5461 tccatccctc cgccttctta cccgctcgtg cccgtttgtg tctccggttg cgtgcggttc
     5521 gcagacgggg gaagggggtt ggtcgaaaag gcggcggcag gacgacctcc cgctgccgtc
     5581 accctgaacc cgtttcaggg cccagatttc cgcccgcgac gcgcttccac cctggatgct
     5641 caaacaagtt cagcatgacg gattgcgaga aagctgtgga aaactcattt cggggccgcg
     5701 aagtcgctgt aatcgctgta tttcgtatcc ggagcctgtg gcgcgagagt gacagtccgc
     5761 aagccctatt acctatgtga acttttccca ttgccgccca tcccgaaacc tgattcattt
     5821 atgaacgggg agcggggtct tggttaagcg gctggtaacc gtttcaatcg catgatctcc
     5881 gccgggtaag agagtgggag tgggatcatg gcgacgaata cgaagccggc cgacggtgcc
     5941 ctgactttgg tggagatgaa ggaattcgcg ggtttcccgg cggcgaccca gcgctacatc
     6001 cgccgttcgc tcgacgtagg actcggccgc cgggacgcgc tccggcgctg gtcgcgcgat
     6061 gtcggcgagg ccgcgtcgat ccgtgcccag gagcgcatgt accggcggct cgatcacgtc
     6121 cgcgccagcg ttcccgacga cagcggcctg gacgagatgg agccggtgat ggggcctttg
     6181 gtcaccatga ccgccttcga cctcggccag gaccggcttc ccgatttcgc ttcctatcgc
     6241 ttcctctatg agcggctgat cggagccgcg gtccgtccgt ggctgcccgg cgctttctgt
     6301 gccgccgccg ctttgccgca cctccacccg gagaagcgcc gcgccctgct gcagtcgatc
     6361 agcgaagccg ccgcgacggc gccgggctgg tcaaaccgcg agcccgtctt ctttcccgag
     6421 tgggtcgaca aggtcgacgt caccgtcgcg gggtgaatca acttaaattc cgggcttcct
     6481 ctcggatgac ggaacggcgg gcccgcgggt ccgccgtttc tatttggggc tgacgatggt
     6541 tatttgagcc agccctgctc gcggtaccag tcggccgttt ccttgaggcc ggtgggggtc
     6601 ttgacctgcg gccgccaaag gcgctcgggc gggcgcttct cggcggcggc tacccagtcg
     6661 gggtggcaga aatagccgac ccggtcgcgg gtgagcttgg cattgcggcg gcggaagagc
     6721 cggtcgagcc cggaggcgat tcgaagcacc ggcccgggca tggccaaggt catcgcccgg
     6781 cggccgtaga tccggccgag ggtccgggcg aaatggcggt gctgccaccc gccctcgcgg
     6841 ccatcgtccg gctcgtaggt ctcggcccag ctgtcggcgt cgtcgagcga ggccaggatg
     6901 agcctgcaca gatcctcgac atggatgacc gagaagcggc cgccgggcgg caaggcgacg
     6961 agcccgcgcc gggccatctt gaacaagtcg agcgtctccc ggtcgcccgg gccgtagatc
     7021 gccggcggcc ggacgatcgt ccagtcgagg cccgaggccg cgaccagccg ctccgacctc
     7081 gccttggacc agccgtaggc ggagagctgc ggctctcgag cggccagcga ggagatgtgg
     7141 acgaagcggc gcacgcccgc cttgcgtgcg gcgtcgatca tggcggcggt gccggcgacg
     7201 ttgaccgctt cgaagccggc gcggccctgc gcgttgatgg cgcccgcgac atggatgacg
     7261 gcgtcggcgc cgatgcataa tttgaggagg gtctcgggcc ggtcgagggc gccgtcgacc
     7321 caggcgatct cgtcctcggg cggcttccag ccgcgggtga gggcccgaac gtcataacct
     7381 tcgccgagcg ccatgcgcag caggtgcgag ccgacgaagc cggtgccgcc ggtgacggcg
     7441 agggttcgga tcttgtgagc tttgcggggc atggcctcta aaatcctccc cggcttttgc
     7501 gggggtgggg gaccgcccga agggcggtgg aggggttctt tccggcctcg aagaacccct
     7561 ccaccgcgct gcgcgcggtc cccctccccc ggaaatccgg gggaggattc aaagaggcga
     7621 ccatgagccc ggctcttcca cgtccgtatc ggctgccttc gcagcgatga tgtgcgaaag
     7681 cagcgcgtcg agcacggcct ccactccggc ccccgtcacc ccggagaggg gcaggggcct
     7741 cgcgcccgct tccgcctcga gctcgtcgga gagagcggcg atcagctcgt cgtcgagcgt
     7801 gtcggacttg ttgagggcga tcacctcggg cttttcggca aggccggcgc cataggcttc
     7861 gagctcctcg cggacgatcc ggtaggtctc ggcgacatcc tccacctcgg agtcgacgag
     7921 gtggagaagg acccggcaac gctcgatatg gccgagaaag cggtcgccga tgccggcgcc
     7981 ttcggcggcg ccctcgatca ggcccgggat gtcggcgacg acgaactccc gctcgcggtg
     8041 gcggacgact ccgagctttg ggtggagggt cgtgaaggga taggcgccga ccttggcctg
     8101 ggcgttggtg accgcgttga ggaaggtcga tttccccgca tttgggaggc cgacaaggcc
     8161 gacgtcggcg agcagcttca ggcgaagcca cacccacatc tcctcgcccg gccagccgtc
     8221 gccgtgctgg cggggcgccc ggttggtggc ggtcttgtag ctggcattgc cgcggccgcc
     8281 gtcgccgccc ttcaggaaga cctggcgctg gccgacccgg gtgaagtcgg cgaggacatg
     8341 ctccttgtcc tcggagagga tctgggttcc gaccgggacc ttgatcagga gatcgtcgcc
     8401 gccggccccg gtgcggttct ggccggcgcc gcccttgccg cgcggcgccc ggaaatgctg
     8461 ggtgtagcgg aagtcgatga gggtgttgag gccgtccacc gcctcgaaga cgatgtcgcc
     8521 gcccttgccg ccgtcgccgc cgtccggccc gccatattcg atatattttt cgcggcggaa
     8581 gccgaccgcg cccgggccgc cggcgccgga gctgatgaaa atcttggcct ggtcgagaaa
     8641 gtgcatggcg ggcatgtagg ggataggctt cccaacgtca tccccgccgc gtgggaatga
     8701 cgtcaggagc gcaaggcggc gagctggacc aggtggcggg tcgcgatctc gcgcgcctgc
     8761 tcccggggaa gcccgagcgc gctcgccatc ggtccgccca tcagcgaatc gcccagagcg
     8821 gcgagcacga gccacagagt cgtctcgtgc accggccggt cctcatggcc cgccccgagc
     8881 tggtcgacga gccggtggat ggcgtcgagg atcggatcga gcgcgtcccg attgccgctg
     8941 aggatcatcc aggcggcaag cgctccggcg ccctcgcggt cgaaagcgtc gaaagtgcgg
     9001 tcgacgatct cgcgcggatc ggcctcgccc tgccgctcgc gctcgaccac ccgggcgatc
     9061 tcggccgtca cccgctcgcc gatcagccgg gccagctcgc cctgaagccc ggccgccgac
     9121 ccgaagtggt ggaggagatt gccgtgcgtc ttgccgatcc gggcgccgac cgccttcagg
     9181 gtcaccgcct gcggccccga ttcgatcagc agcgcacggg ccgcctcgag cgccgcggcc
     9241 cggctctcct ccggcgagag gcgttcacgt cctattgaca tttgtgtaag tatgtcgcat
     9301 cgctcatagc gaaggagata cagccatggg gcgggaagtc acaccctccg atctgacgat
     9361 tacgccgcgg gaccgccgct tcggccgcgg ggccacgacg gagcgttggt ggctgggcgg
     9421 caatccctat ggcagcgccc tctacgacgc gctctcggtt accttcccca agggcgaggc
     9481 cttcttcgtc gagagcgtcc gcgccttccg cgacgacgct ccgcccaggc tcgccgagga
     9541 gatccgcgcc ttcaccaccc aggaggtgat gcacagccgc gagcatgtcg ccttcaaccg
     9601 gcgcgcggtc gaggccggct acgatatcgc cccgctggag gcgcgggtcg actatcggat
     9661 cgaggagacg aagagccggc cgccgatcgt gaacctcgcc gccaccatgg cgctcgagca
     9721 tttcaccgcc atcctcgcca accagctgct cggcaatccc cgccacctga agggcgccga
     9781 tccccagtcg gccgccttgt ggcgctggca ctcgatggag gagatcgagc ataagggcgt
     9841 cgcctacgac acctggctcc acgccacccg caactggccg cgccgcaagc gctggatggt
     9901 gaaatcgctg gtgatgctca aggtgaccaa gaacttcttc gtcgagcgca cccagggcgc
     9961 cctgatgctt ctcgagcagg acgggatcag gggcccccgc gcgtgggccg gcctgttctg
    10021 gtatgcgatg gtcagccccg gcatgttccg gaagatcctc gtcggctggc tgaagttctt
    10081 tctgcccgga ttccacccct ggaagcatga cgaccgcaag ctgatcgccg agggcgagcg
    10141 cagcctggag gctgccgccg cttgatctgc ctccccatcg cagatgggga gggggacccc
    10201 gcctagcggg gtggaggggt aattggcgca ggcttctgcc agctacccct ccaccaccc
//
LOCUS       DASWNU010000609         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_256537, whole genome shotgun sequence.
ACCESSION   DASWNU010000609 DASWNU010000000
VERSION     DASWNU010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256537"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1160
                     /locus_tag="VGB57_12170"
     CDS             111..1160
                     /locus_tag="VGB57_12170"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0132149.1"
                     /translation="MSASSKAQTPPPATAPAPTIDAAARRAVLEAAITRITANYVFPD
                     KTAAIAKALRRKLSSGGFDTIGDPKAFARAVNAVIEAVAHDKHLGLTWSPDPLPPLGA
                     GGEPDAATIAMHEAMMRRINYAIPKAEVLDGNIGYLKMNLFARAQDAGPTLAAAMAFL
                     QHTDALVFDLRDNGGGDPGMVAMALSYLVPPQTEINRFHQRGKDVDEQVWSLPYVPGG
                     RWSTDKPVYVLTSKETASAGEEFAYDLQQMKRGTIVGGSTWGGANPGDFLPIDRHFAI
                     FVPTGAAVNPISKTNWEGVGVKPDVAVDPASALETARRLALEKLLATATGERARELRG
                     ILQARSAPPADPPSN"
     gene            1169..1708
                     /locus_tag="VGB57_12175"
     CDS             1169..1708
                     /locus_tag="VGB57_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132150.1"
                     /translation="MLRIVGYAIFLYLLLKLFRLMVRPAAGDRDTETTWRTVLRAGEP
                     MLSIGIFGFAAMIAIYTTWWGQRFAEGQYTYSTDCYARMAASHHLPGRPVRFGSYNAA
                     QAATGHLRFAEIHGSQLGISKEVIERKLDQARIGYSEYYGGLAAKGSRQGIAASVADL
                     DRCLKGEGAPRGELLTPNV"
     gene            complement(1792..2667)
                     /locus_tag="VGB57_12180"
     CDS             complement(1792..2667)
                     /locus_tag="VGB57_12180"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0132151.1"
                     /translation="MSATEPRSIWNVAAELGEGPVWVDRDRALWFTDIKKREIHRFDP
                     AAGDRRTWVAPEQVGFVLPADGGGFVAGLMSGLHRFDESTGRFTLIAGMEPERPNNRL
                     NDGCVDPAGRLWFGTMDNGETAKTGAFYRFERGSVTPTGLQGIAITNGPALSPDGRIL
                     YFVDTREGTIAAAEVGKEGALGPSRPFARIDPAEGYPDGPTVDSEGCVWISLYAGWEA
                     RRYSPAGELVGRVRFPVANITKIAFGGEDLRTVYATTARQLLSPTEIERQPQAGDLFE
                     FRVDVPGLPCPLVAA"
     gene            2799..3122
                     /locus_tag="VGB57_12185"
     CDS             2799..3122
                     /locus_tag="VGB57_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132152.1"
                     /translation="MPDAPTVYPLRDLFDVAVRGRAVRLTCARCGHGTVFSSHALWWL
                     FRRKGWVDSLREVRRRCLCIMCLHRRGTKVRDPELELVDEAPTDNSLPMPSELDWKRE
                     VRRRR"
BASE COUNT          533 a         1035 c         1057 g          521 t
ORIGIN      
        1 ccacgcgacg ccgatcgaca tgaggctttc gaaccctggc cgggaggacc ccgatgagat
       61 ctgatcgaat ccgttttttg cttgcatcgg cgctcctctc ggcgactgcc gtgagcgcct
      121 cttcgaaggc gcagacgccg ccgcctgcga ccgcgcccgc tccgacgatc gatgccgcgg
      181 cacgcagggc ggtgctcgaa gccgctatta ccaggatcac cgcgaactac gtctttcccg
      241 acaagacggc cgccatcgcg aaggcgctcc gacgcaaatt atcctcgggc ggtttcgaca
      301 cgatcggcga ccccaaggcc ttcgcccgcg cggtgaatgc cgtcatcgag gctgtggcac
      361 acgacaagca cctcggcctc acctggtcgc cggacccgtt gccgccgctg ggcgccggcg
      421 gcgagccgga cgcggccacg atcgcgatgc atgaggcgat gatgcgccgg atcaactatg
      481 ccattcccaa agcggaagtg ctcgacggca acatcggcta tctgaaaatg aacctgttcg
      541 cccgcgccca ggatgccggg ccgaccctgg ccgcggcgat ggcgtttctg cagcataccg
      601 acgcgctggt cttcgacctg cgcgacaacg gcggcggcga tccgggaatg gtggcgatgg
      661 ccctgagcta tctcgtgccg ccccagaccg agatcaatcg tttccaccag cgcggcaagg
      721 atgtggacga gcaagtctgg tccctgccct acgtgccggg cggacggtgg agcaccgaca
      781 agccggtcta tgtcctgacc agcaaggaga cggcatcggc cggagaggag ttcgcctacg
      841 atctgcaaca gatgaagcgc ggcacgatcg tcggcggctc gacgtggggc ggcgccaacc
      901 cgggcgactt cttgccgatc gaccggcatt tcgccatctt cgtgcccacc ggggcagcgg
      961 tgaatcccat ctcgaagacc aactgggaag gcgtgggcgt gaagcccgat gtcgccgtcg
     1021 atcccgcatc ggcgctcgag accgcccgtc gtctggcgct ggaaaagctg cttgcgaccg
     1081 cgacgggaga gcgtgcgagg gaattgcggg gaatattgca ggcccggtcg gctccaccgg
     1141 cagatccacc ctccaactga gagcgcccat gcttcgaatc gtcggctacg cgatcttcct
     1201 gtatctgctg ctcaagctat ttcgtttgat ggtgaggccg gccgccggag atcgcgacac
     1261 cgaaacgacc tggagaaccg tgctacgagc aggcgagccg atgctttcca tcggtatatt
     1321 cggtttcgcc gccatgattg cgatctacac gacatggtgg gggcagcggt tcgccgaggg
     1381 ccagtacact tactcgaccg actgttatgc ccgaatggcc gcttcgcacc atctgcccgg
     1441 caggccggtg agatttggtt cgtacaatgc cgcgcaggca gcgaccgggc acctcaggtt
     1501 cgccgagata cacgggtctc agcttggaat aagcaaagag gtcatcgaaa ggaaactgga
     1561 tcaggcgcgg atcggctatt ccgaatatta tggcggcctg gcggcaaagg gctcccgcca
     1621 agggatagcc gcctcagtcg ccgatctgga tcgatgcctg aaaggcgaag gggctccccg
     1681 cggagagctt ctgacgccta acgtttaggc tcgcgctcag gcacatcgtt cgagattgag
     1741 gcacgggcgg ccggacgccg cctcggccgt caccctgaac ctgcatcctc ttcaggccgc
     1801 caccagcggg caaggcaatc ccggcacatc gacccgaaac tcgaacaggt cgcccgcctg
     1861 cggctggcgc tcgatctccg tcggggacag gagctggcgg gcggtggtgg catagacggt
     1921 gcgcaggtcc tcgccgccga aggcgatctt ggtgatgttg gcgacgggga agcggacccg
     1981 gcccaccagc tcccccgccg gcgaatagcg gcgcgcctcc cagccggcgt agaggctgat
     2041 ccagacgcac ccttcgctgt ccacggtcgg gccgtcggga tagccttcgg ccggatcgat
     2101 tcgcgcgaac ggccgggaag gcccgagggc gccttccttg ccgacttcgg cggccgcgat
     2161 cgttccttcg cgggtgtcga cgaaatagag gatccggccg tcgggcgaca aggccgggcc
     2221 gttggtgatc gcgatcccct ggaggccggt cggagtgacg gagccgcgct cgaagcggta
     2281 gaaggcgccg gtcttcgccg tctcgccatt gtccatcgtc ccgaaccaga gccgtccggc
     2341 ggggtcgaca cacccgtcgt tgagccggtt gttcggccgc tcgggctcca tcccggcgat
     2401 cagggtgaag cggccggtcg actcgtcgaa gcggtgaagc ccgctcatca gcccggcgac
     2461 gaacccgccc ccgtcggccg ggaggacgaa gccgacctgc tcgggcgcga cccatgtccg
     2521 gcggtcgccg gcggcaggat cgaagcggtg gatctcgcgc ttcttgatgt cggtgaacca
     2581 caaggcgcgg tcgcgatcga cccacaccgg cccctcgccg agctcggcgg cgacgttcca
     2641 gatgcttctc ggttcggtgg cggacatggc tgacgctcct gccacaagcc ggcgcaacgt
     2701 caatgggttc cgaagccaaa gctcgtcggg aagtctaaat cgcccaggag ccgggggacg
     2761 acttccatga catcgttttg ttcgctattt gttctccaat gcccgacgcg ccgaccgttt
     2821 atcccctgcg cgacctgttc gacgtcgccg tgcggggtcg ggcggtgcgg ctgacctgcg
     2881 ctcgctgcgg gcacgggacg gtgttcagca gccatgcctt gtggtggctg ttccgccgca
     2941 agggctgggt ggactcgctc cgcgaggtcc gccgccgctg cctgtgcatc atgtgcctgc
     3001 accggcgggg gaccaaggtt cgcgacccgg agctcgagct ggtcgacgag gcgccgaccg
     3061 acaacagcct gcccatgccc tcggagctgg actggaagcg cgaggtcagg cggcgccggt
     3121 gagggatggc gggccgaggt ggattg
//
LOCUS       DASWNU010000610         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_177912, whole genome shotgun sequence.
ACCESSION   DASWNU010000610 DASWNU010000000
VERSION     DASWNU010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177912"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..94
                     /locus_tag="VGB57_12190"
     CDS             <1..94
                     /locus_tag="VGB57_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY0132153.1"
                     /translation="GRVMAAMKERHAGSLDMSKASALVKARLSA"
     gene            104..1954
                     /gene="dnaG"
                     /locus_tag="VGB57_12195"
     CDS             104..1954
                     /gene="dnaG"
                     /locus_tag="VGB57_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0132154.1"
                     /translation="MSLSPQFLDELRARTSLSALVGRTVKLQRAGREWKACCPFHKEK
                     TPSFYVNDEKGFAHCFGCSAHVDAIGWLTEARGLPFMDAVKELAEAAGMEVPAADPRA
                     RERAEKATGLYDVMTAAQNWFEEQLGGLEGAEARAYLEKRGISEATRRRFGFGFAPDG
                     RGKLRTALRNFGNEALVEAGLLVVPDDDREPYDRFRGRLMFPIRDRRGRVVAFSARIL
                     GPGEPKYLNSPDTPLFDKGRTLFNIDKAAAASREARRVIVVEGQMDAIALDQAGIGEA
                     VAPLGTAVTEAQLGLLWRLSPTPIFCFDGDSAGQKAAVRALMRALPHVGPGRSLSFAT
                     LPAGQDPDDLIRAGGRQGIESLLEAPQSLVDRLWNHELNAEPLATPEQRAGLRQRLSD
                     HVATIQDPHVREQYRSELLGRFNALTAPPPRPPWKPRPFGPAGSRGGGKFVPRRPVSD
                     RARALASAGISPKTARSVLHGLLRFPELVAAHSEAIAALPIPDEMAARLRDALLEGAM
                     AHGELDQEQVHTILLEAGVAPLAERLRLERGLAFSFNRRDADPERASRDLVLVIDTLA
                     AQPGLYAALDAATARLKEEGDEAAFQEQQRLRAARDEADRQLAALIESGA"
     gene            1979..>2253
                     /locus_tag="VGB57_12200"
     CDS             1979..>2253
                     /locus_tag="VGB57_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEY0132155.1"
                     /translation="MAKTNTQAETDDKQDNGDAPLIDLNEASLKKLVARAKKRGYITY
                     DELNSALPQDMSSDQIEDIQTALSEMGVSIVETDEDAAEEDSENEAE"
BASE COUNT          384 a          773 c          765 g          331 t
ORIGIN      
        1 gggccgggtg atggcggcga tgaaggagcg gcacgccggc tcgctcgaca tgtcgaaggc
       61 gagcgcgctg gtgaaggccc gcctttccgc ttaggctcgg ggcatgtccc tcagccccca
      121 gtttctcgac gagctgcgcg cccgcacgtc gctgtcggcg ctggtcgggc ggacggtcaa
      181 gcttcagcgc gccgggcggg aatggaaggc ctgctgcccc ttccacaagg agaagacccc
      241 gagcttctac gtcaatgacg agaagggctt tgcccattgt ttcggttgct ccgcccatgt
      301 ggacgcgatc ggctggctga ccgaggcccg tgggctaccc ttcatggacg cggtgaagga
      361 gctcgccgag gccgccggga tggaggtgcc ggcggcggac ccgcgcgcac gggagcgggc
      421 ggaaaaggcg accggccttt atgacgtcat gaccgcagcc cagaattggt tcgaagagca
      481 actcggcggt ctcgaggggg cggaggctcg cgcgtacctc gagaaacgcg ggatttccga
      541 ggcgacccgg cgccgcttcg gcttcggttt cgctccggac gggcgcggca agcttcgcac
      601 ggcgctcagg aatttcggta atgaagcgct ggtcgaggcc ggcctgctcg tcgttccgga
      661 cgacgatcgc gagccttacg accgcttccg cgggcggctg atgttcccga tccgggaccg
      721 gcgcggccgg gtcgtcgcct tcagcgcccg gatactcggc cccggcgagc ccaaatatct
      781 caactcgccc gacaccccct tgttcgacaa gggccgaacc cttttcaaca tcgacaaggc
      841 cgcggccgcc agccgggagg cgagacgggt gatcgtcgtc gaaggccaga tggacgcgat
      901 cgccctcgac caggccggca tcggcgaagc cgtcgctccg ctgggcaccg ccgtcaccga
      961 ggcccagctg ggcttgctct ggcgcctctc ccctaccccc atcttctgct tcgacggcga
     1021 ttccgccggc cagaaagcgg cggttcgggc gctgatgagg gccctgcccc atgtcgggcc
     1081 gggacgctcg ctcagcttcg cgaccctccc cgccggccag gatcccgacg acctcatccg
     1141 ggccgggggc cgccagggga tcgaatcgct gctggaggct ccgcaatcgc tcgtcgaccg
     1201 gctctggaac catgagctga acgccgagcc gctcgcgacg cccgagcagc gcgccggcct
     1261 caggcagcgc ctcagcgacc atgtcgcgac gatccaggac ccgcatgtgc gcgagcaata
     1321 tcgctccgaa ttgctcggcc gcttcaacgc cctcaccgcg ccgccgccgc ggccgccgtg
     1381 gaagccgcgc cccttcggcc cagccggctc ccgcggaggt ggaaagttcg ttccccggcg
     1441 tcccgtgtcg gaccgggcca gggctctggc cagcgccggg atcagcccga agaccgcccg
     1501 ctcggttctc cacggactgc tccgttttcc ggagctggtc gcggcccatt ccgaggcgat
     1561 cgccgcgctt ccaatccccg acgagatggc cgcccggctg cgcgacgccc tcctcgaagg
     1621 cgcgatggcg cacggcgagc ttgatcagga gcaggttcat accatattgc ttgaggctgg
     1681 cgtcgcccct ctcgccgaga ggttgcgcct ggaacgagga ctggccttct ccttcaaccg
     1741 ccgtgatgcc gacccggaac gtgcttctcg cgatctcgtt ctggtcatcg acacattggc
     1801 ggcccaaccg ggactatatg ccgcgctcga cgcggcgacc gctcggctca aggaggaagg
     1861 cgacgaagcc gccttccagg agcagcagag attgcgggcg gcgagggacg aggcggacag
     1921 gcagctggcg gcgctgatcg agagcggcgc ttagcggatg aaaatataag gcgtttagat
     1981 ggcgaagacg aacacgcagg ccgagaccga cgataagcag gacaacggcg acgcgccgct
     2041 gatcgacctc aacgaggcct cgctcaagaa gcttgtcgcc cgcgccaaga agcgcggcta
     2101 catcacctac gacgagctca acagcgcgct gccgcaggac atgagctccg accagatcga
     2161 ggacatccag accgcactga gcgagatggg cgtcagcatc gtcgagacgg acgaagacgc
     2221 ggccgaggaa gacagcgaga acgaggcgga aga
//
LOCUS       DASWNU010000611         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_283339, whole genome shotgun sequence.
ACCESSION   DASWNU010000611 DASWNU010000000
VERSION     DASWNU010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283339"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1212)
                     /locus_tag="VGB57_12205"
     CDS             complement(10..1212)
                     /locus_tag="VGB57_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEY0132156.1"
                     /translation="MGVVAQLKSKTENRLGRSDRSAAGRWFWEIDRVLLFFVAVLIAI
                     GLIAVAAASPAAGQRLSGGGVSYAPLHYFYRQLVWIVVALPVMVAVSMLPRTKAKRWC
                     LIGTLFFTGLLFLVPIMGVEVNGARRWLGMGFAQFQPSEFLKPLYIVSIAWLLSLKEQ
                     DASLPVVPLSGICTGLIALLLMQQPNFGETIIFAAAWVMLLTLSGVSLRTLGFLAIGG
                     IVAIVMAYFFYPVATTRIDAFLFSGAGGHDNYQTESALRTLTAGGLFGAGPGAGVRKF
                     HLPEPHTDYIFSVIGEEFGIIACLAIAVIYLVIVARVLIRLLHEEDKFVVLAAAGLVC
                     QFGLQALINMAVNVQLAPSKGMTLPFISYGGSSMVALSIGMGLLLAFTRRNPYLHRSP
                     YVVKWSGR"
     gene            complement(1212..2489)
                     /gene="murD"
                     /locus_tag="VGB57_12210"
     CDS             complement(1212..2489)
                     /gene="murD"
                     /locus_tag="VGB57_12210"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0132157.1"
                     /translation="MITSPAFSGRKYSVYGLARSGLATVEALLASGAEVTAWDQNEAA
                     RERVRELGLSMADPLTAPLDGFDGIVVSPGVPLNRHPIAARAREAGVPLIGDIELFAL
                     ARPTLPSHLVVGITGTNGKSTTTALVHHIVRTAGRPSTMAGNIGFPILGQEPLPEGGV
                     YVLELSSYQIDLTRGLDCDVSVLLNITPDHLDRYDGVEDYAASKARLFAMQSPGHVAV
                     IAAEDDYARSIAGEVEGRKVLVSSDQVKDQSLWPALQGPHNAQNIAAAVAVAKALGIG
                     DEPIARGLATYPGLPHRMERIAEKNGVLFVNDSKATNPTSAAPALAAYPAVHWILGGL
                     PKSDDLDACEPYLGHVRGAYTIGEAGPMFARLLRSKVPVSECEMLFEAVAEAAEKAKP
                     GEVVLLSPACASFDQFRDYEARGDAFRAAVEAL"
     gene            complement(2623..>3439)
                     /gene="mraY"
                     /locus_tag="VGB57_12215"
     CDS             complement(2623..>3439)
                     /gene="mraY"
                     /locus_tag="VGB57_12215"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY0132158.1"
                     /translation="SMLLWMDFRNTFLWACLFITVGFGLIGFLDDYDKVTKKSHKGVS
                     ARVRLLAEFLVAGIGCTIITWGAGTQLYIPFYNGPVVDLGFFYPFFGAFVVVAFGNAV
                     NLTDGLDGLATMPVIIASLAFLVISYVAGNAVFAEYLGIPFVQGAGNLPILCSAIVGA
                     GLAFLWFNAPPAAVFMGDTGSLALGGALGAIAVATQHEIVLGIIGGLFVVEAMSVIIQ
                     VFFYKRTGRRIFKMAPIHHHFEQIGWSEATIVIRFWIIAFVLALAGLSTLKLR"
BASE COUNT          668 a         1108 c         1171 g          492 t
ORIGIN      
        1 taaaactcct catcgaccgc tccatttcac cacataaggc gaccggtgca gatacggatt
       61 ccgcctcgtg aaggcgagca gaagccccat cccgatcgac agcgccacca tggacgagcc
      121 gccgtagctg ataaacggca gggtcatgcc cttcgagggc gcaagctgca cgttgacggc
      181 catgttgatc agcgcctgga gcccgaactg gcagacgagg ccggcggcgg cgaggacgac
      241 gaacttgtcc tcctcgtgga gcagccggat cagcacccgg gcgacgatga cgaggtagat
      301 gaccgcgatg gcgaggcagg cgatgatccc gaactcctcg ccgatcaccg aaaagatata
      361 gtcggtatgg ggctccggca gatggaactt gcgcacgccc gcccccggtc cggcgccgaa
      421 cagcccgccg gcggtcaggg tcctcaaggc cgactcggtc tgataattat cgtgcccgcc
      481 ggcgccgctg aacaggaagg cgtcgatccg ggtcgtcgcg acgggataga agaaataggc
      541 catgacgatc gcgacaatgc cgccgatcgc gaggaacccg agggtcctca gcgagacgcc
      601 ggacaaggtg aggagcatca cccaggccgc cgcgaagatg atcgtctcgc cgaaattggg
      661 ctgctgcatg aggagcaagg cgatcagccc ggtgcagatt ccggacaagg gcaccaccgg
      721 caggctggcg tcctgctcct tgagcgaaag cagccaggcg atcgagacga tgtagagcgg
      781 cttcaggaat tccgacggct ggaactgggc gaagcccatt ccgagccagc gccgggcgcc
      841 gttcacctcg acacccatga tcgggaccag gaaaagcagg ccggtgaaga aaagggtgcc
      901 gatcaggcac cagcgcttcg ccttggtcct gggcagcatc gagacggcga ccatcaccgg
      961 cagtgcgacg acgatccaga ccagctgccg gtagaaataa tggagcggcg cgtagctcac
     1021 gcctccgccc gacaggcgct ggccggcggc gggcgaggcg gcggcgacgg cgatgagccc
     1081 gatcgcgatc agcaccgcga cgaagaagag gaggacccgg tcgatctccc agaaccagcg
     1141 cccggcggcg cttcggtccg accgcccgag ccggttctcg gtcttgctct tcagctgtgc
     1201 gactacgccc attacaacgc ctccaccgcg gcccggaaag catccccccg cgcctcataa
     1261 tcccggaact ggtcgaacga ggcgcaggcc ggcgacagaa gcacgacctc gcccggcttc
     1321 gccttctccg cggcctcggc cacggcctcg aacagcatct cgcattcgct gaccggcacc
     1381 ttggagcgaa gaagcctggc gaacatcggt ccggcctcgc cgatcgtgta ggcgccccgg
     1441 acatggccca gatagggctc gcaggcgtcg agatcgtccg atttgggaag cccgcccaaa
     1501 atccagtgca ccgccggata ggccgcaagc gccggagccg ccgaggtcgg gttggtcgcc
     1561 ttgctgtcgt tgacgaaaag cactccgttc ttctcggcga tccgctccat ccggtggggc
     1621 aggccgggat aggtggcgag gccgcgcgcg atcggctcgt cgccgatccc gagcgccttc
     1681 gcgaccgcca cggcggcggc gatgttctgc gcgttgtgcg gcccctgcaa cgcgggccag
     1741 agcgactggt ccttcacctg gtccgaggag accaggacct tgcgcccttc cacctcgccg
     1801 gcgatggagc gcgcataatc gtcttcggcg gcgatcaccg cgacatggcc cggcgactgc
     1861 atcgcgaaca gccgcgcctt cgaggcggca taatcctcga ccccgtcata gcgatcgaga
     1921 tggtccgggg tgatgttgag aagcaccgag acgtcgcagt cgaggccgcg ggtcaggtcg
     1981 atctgatagg aggagagctc gagcacatag actccgccct ccgggagcgg ctcctggccc
     2041 aggattggga agccgatatt gccggccatg gtcgacgggc ggccggccgt ccggacgatg
     2101 tgatggacca aagcggtggt cgtcgacttg ccgttggtgc cggtgatccc gaccacgagg
     2161 tgcgatggaa gcgtcggccg ggccaaagcg aaaagctcga tgtcgccgat cagcggcacc
     2221 cccgcttccc gcgcccgagc cgcgatcggg tggcggttca gcggcacgcc gggggagacg
     2281 acgatcccgt cgaagccgtc gagcggggcg gtcagcggat cggccatgct gaggccgagt
     2341 tcccgcaccc gctcgcgcgc cgcctcgttc tggtcccacg ccgtcacttc ggcgccgctg
     2401 gcgagcaacg cctcgacggt cgccaggccg gagcgggcga ggccgtagac ggaatatttg
     2461 cggccggaaa aggcggggct ggtaatcatg ccgtcacccc ggcggaagcc ggggcctcgg
     2521 ggagacccgc cgagacttgt cgtcccggga tcccggcttt cgggttccca tcaaagtagt
     2581 actttgatgg ggtccctccg gggatgacga ggggagcttc gatcatctca gcttcagggt
     2641 cgacaggccg gcgagggcca gcacgaaggc gatgatccag aagcggatga cgatggtcgc
     2701 ctcggaccag ccgatctgct cgaaatgatg gtggatcggc gccatcttga agatccgccg
     2761 gccggtccgc ttgtagaaga agacctggat gatgacgctc atcgcctcga ccacgaacag
     2821 tccgccgatg atcccgagca cgatctcgtg ctgggtcgcg accgcgatcg ccccaagcgc
     2881 cccgcccagc gcgaggctcc ccgtgtcgcc catgaagacg gcggccggag gcgcgttgaa
     2941 ccagaggaag gcgaggccgg cgccgacgat cgccgagcag aggatgggca gattgcccgc
     3001 gccctgcacg aacgggattc cgagatattc ggcgaagacg gcgttgccgg cgacgtagga
     3061 gatgacgagg aaggcgaggc tggcgatgat caccggcatg gtggcgagcc cgtcaagccc
     3121 gtcggtgagg ttcaccgcgt tgccgaacgc caccaccacg aacgctccga agaagggata
     3181 gaagaagccg aggtcgacga cggggccatt gtagaagggg atgtagagct gggtcccggc
     3241 gccccaggtg atgatggtgc agccgatgcc ggcgacgagg aactccgcga gcaggcgcac
     3301 ccgagccgag acgcccttgt ggctcttctt cgtgaccttg tcgtaatcgt cgaggaagcc
     3361 gatcagcccg aagccgacgg taatgaacag gcaggcccac aggaaggtgt tgcggaaatc
     3421 catccacaaa agcatcgag
//
LOCUS       DASWNU010000612         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_361308, whole genome shotgun sequence.
ACCESSION   DASWNU010000612 DASWNU010000000
VERSION     DASWNU010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361308"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VGB57_12220"
     CDS             complement(<1..976)
                     /locus_tag="VGB57_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0132159.1"
                     /translation="MAGVLSGIRIIELAGIGPAPFAGMMLADHGATVIRVEREDRPPV
                     IPPGFDILGRSRASIVRVDLKSEKGVARIRELARDADGLIEGFRPGVMERLGLGPDLL
                     LEDNPRLVYGRMTGWGQDGPLAQSAGHDINYIALAGALHTYGRAGGPPTPPVNAVGDF
                     GGGGMLLAFGMLAGILSARTTGKGQVIDCAMVDGAALLSALTWSLKAAGMWKDERGVN
                     LLDTGRAYYDVYQCADGEWVAVGALEPEFFAVLKERLGLECGQHDAGLREELAAAFLR
                     HDRQHWCNILEHCDACFAPILSLSEAPGHPHNAARGTFLDIAGVIQPAP"
     gene            1166..>1658
                     /locus_tag="VGB57_12225"
     CDS             1166..>1658
                     /locus_tag="VGB57_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132160.1"
                     /translation="MAEIYGTSGNDNLVGTPDSDTFHPLLGQDTVDGLGGSDTLVVDY
                     SGFEFVDPYGGAAVPDRPRYAVTSSGGSFAGAVSGGGTVTFSNVEHLQFALSGNPDEF
                     VIDASAVALGATVRVDGGRGYDRLRADLSALASFDLSVGSGPNSASFTVVNFEEFALD
                     LTAG"
BASE COUNT          263 a          576 c          566 g          253 t
ORIGIN      
        1 cgggggcggg ctggatcact ccggcgatgt cgaggaaggt gccgcgggcg gcgttgtggg
       61 ggtggccggg ggcctcggaa agcgagagga tcggggcgaa acaggcgtcg caatgttcga
      121 ggatgttgca ccaatgttgg cgatcgtggc ggaggaaggc ggcggcgagc tcctcccgaa
      181 ggccggcatc gtgctggccg cactccaggc ccagcctctc cttcaggacg gcgaagaatt
      241 cgggctccag cgccccgacc gccacccact ccccgtcggc gcactggtag acgtcgtaat
      301 aggcccggcc ggtgtcgagc agattgaccc cgcgctcgtc cttccacatc ccggcagcct
      361 tcagcgacca ggtcagcgcc gacagcaggg ccgccccgtc gaccatcgcg cagtcgatca
      421 cctggccctt gcccgtcgtc cgggcggaca ggatccccgc cagcatcccg aaggcgagaa
      481 gcatcccgcc gccgccgaaa tccccgaccg cgttgaccgg cggcgtgggc ggcccgccgg
      541 cgcggccgta ggtgtggagc gcgccggcca gagcgatata gttgatgtcg tgcccggccg
      601 actgggcgag cggtccgtcc tggccccagc cggtcatccg gccgtagacg aggcgcggat
      661 tatcctcgag cagcaggtcg gggccgagcc cgagccgctc catcaccccg ggcctaaagc
      721 cttcgatcag gccgtcggcg tcccgggcga gctctcggat ccgggcgacg cctttctcgg
      781 acttgaggtc cacccggacg atggaggcgc gggagcggcc cagaatgtcg aacccgggcg
      841 gaatcaccgg cggccggtcc tcgcgctcga cccggatgac cgtcgcgccg tgatcggcga
      901 gcatcatccc ggcgaacggc gcggggccga ttccggcgag ctcgatgatg cggattccgg
      961 agagcacccc tgccatgatt cctgcataga gcgcctcccg ccgccccgcc acccggcgcg
     1021 gcgacccgcg gcgccgcaat agaaacgccc atcccacctc cgacgccggt ccattccgga
     1081 tgagattgag attgtctttc aactctctgc gctgaatgtg ggcaggtggt cagccatgcc
     1141 ggcgcgacgg cctcaggggg aaatcatggc tgaaatctac ggtaccagcg ggaacgacaa
     1201 tctcgtcgga acgcccgact ccgatacctt ccatcctttg ttgggccagg acacggtcga
     1261 cggcctcggc ggaagcgaca cgctcgtcgt cgactattcc ggcttcgagt tcgtcgaccc
     1321 ttacggcggc gccgctgtgc cggaccggcc gagatacgcc gtcacgtcga gcggcggctc
     1381 cttcgccggg gccgtctcgg gcggcggcac agtcaccttc tccaacgtcg agcacctgca
     1441 gttcgctctc agtggaaatc ccgacgagtt cgtcatcgac gcgagcgcgg tggccttggg
     1501 cgccaccgtc agagtcgatg ggggccgagg atatgaccga ctgagggcgg atctgtccgc
     1561 tctggcgagc ttcgacctga gcgtgggctc cggcccgaac agcgccagct tcacggtcgt
     1621 caacttcgag gagttcgccc tcgacctgac ggcaggcg
//
LOCUS       DASWNU010000613         4882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_440004, whole genome shotgun sequence.
ACCESSION   DASWNU010000613 DASWNU010000000
VERSION     DASWNU010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440004"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1747
                     /locus_tag="VGB57_12230"
     CDS             104..1747
                     /locus_tag="VGB57_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0132161.1"
                     /translation="MRFDDMIATVLRQSEARPSALAAKWRQLVDLVAQRRSEGEAQAE
                     ALAWLRSNRGRVPAEARREVARSLAGRRVSPAALSFFAEDLPAVASPLIGGARLGADE
                     WLDLLPHLGPVARGLLRHRRDLPPDVVHALARFGAGDFALGGEVAAPAVDSLSPAAER
                     SEAQIRDLVARIESFRRRRDAAGAAAAAEPAPVEEFQWETGPDGVILWVEGAPRGPLV
                     GHSIASIAERGQYGVDGQAAGAFEKRAPFRDARFSVPGGGAASGDWRISGVPFFDAAQ
                     GNFLGYRGSARRPRLDEVAHGAAPAEAPAGLFGTDLPADSLRQLIHELRTPLNAIIGF
                     AEMIDGQYMGPAALSYRSRASDIMDQANRLLSAVDDLDTAARLETQRLHLSESSVDAV
                     ALLCRLHDAYERVARQRGSRILIEIERDLPDAKVELDAAERMFARMLAATIGLAREAE
                     TITATMAMGEEGARKMLCLSIDRPRAIEGLDENALLDPGYSPDGDWPGAPALGLGFAL
                     RLVRNLAEAVGGALEIGTGRFFLYLPPLEPAERSSGQNG"
     gene            1754..2779
                     /locus_tag="VGB57_12235"
     CDS             1754..2779
                     /locus_tag="VGB57_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WalW protein"
                     /protein_id="HEY0132162.1"
                     /translation="MAPDDEPDARTGRLDRPPPESSYIALPRSFGRRFTVFCDTEEEF
                     DWAKPRSRDNRSTTHMAAMPDLQERFRRRGVKCVHLVDHPIATDPASVAMLRGWLERD
                     EISVGTQLHPWVNPPHEEEVNTFNSFVGNLPISLERAKIENLTATIESAFGRRPIVYR
                     AGRYGVGPNTAVLLSESGYEADVSVRALHDYSDERGPDFTRVKPFPFRIGGDGLVELP
                     LSAAYVGPLRSRGRSIYRAAGRLPRGRGLLFRTGLLNRAALTPEGVPLEEALAAARAL
                     IEDGVSWFSLSFHSPSVEPGHTPFVRTAEDLRTFHAWWDGMFDFFEREGIAPASIEET
                     IAAAKAA"
     gene            2836..2910
                     /locus_tag="VGB57_12240"
     tRNA            2836..2910
                     /locus_tag="VGB57_12240"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2868..2870,aa:Ile,seq:cat)
     gene            complement(2927..3985)
                     /locus_tag="VGB57_12245"
     CDS             complement(2927..3985)
                     /locus_tag="VGB57_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132163.1"
                     /translation="MDSSGEAGNKERAAILADPAWLPHRVLDEGRTLQLVHLPRDEQR
                     GLSFLDHRFVAEEIPRFELAVAELAGKDKPPTAAGCHFIFHSAFCCSTLMSRALGEAG
                     AATSLHEPQALTDLACLLPKSHWPDDQQQALRVVLDLLERPHQVGGTTVIKPSDFANP
                     LIDAILAFRPGSRILLMYAALPAFLLTIARRGRKYRSWARSLATIYRRHPQFETKRTN
                     DLLLLTDLEVAAFVWLNHQARFVRLAATLPEGRVATLRADSFLAEPARTVHAASAHFG
                     LGLTEEQAAAIAAGSVFGEHSKRPGRSFDPAALKREEALAGFAYGAEIGEAVEWAEAV
                     AAQAGVPLSLEAPLLGRS"
     gene            4083..4159
                     /locus_tag="VGB57_12250"
     tRNA            4083..4159
                     /locus_tag="VGB57_12250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4117..4119,aa:Pro,seq:tgg)
     gene            4249..4638
                     /locus_tag="VGB57_12255"
     CDS             4249..4638
                     /locus_tag="VGB57_12255"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY0132164.1"
                     /translation="MKRPILILLALLAVAVAATTLYLGRRDRGHAAAQPAPPSGPSGE
                     MMGAMHRMHEAANKTAMTGDIDRDFVALMVPHHRSAVEMARVYLEHGRDPALRRLSEN
                     VVASQEAEIRQMSGRAAAPAPGPVPGH"
     gene            complement(4635..>4882)
                     /locus_tag="VGB57_12260"
     CDS             complement(4635..>4882)
                     /locus_tag="VGB57_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0132165.1"
                     /translation="DLARAGGFDAVFTDVVMPGMSGIELAEALAPVLPNVPVVLTTGY
                     SDEISRSGGAGRPVVFKPYRLETLSGVLDSVLEPRIG"
BASE COUNT          732 a         1764 c         1656 g          730 t
ORIGIN      
        1 tattgcgacg ggccgcgaaa gcgcctcttc cgctcgcccg gaggccgcgt taacgattgg
       61 cggggccggc gatcggccct gcgttcacga agggctgggc cgcttgcgtt tcgacgacat
      121 gatcgcgacg gtgctgaggc agagcgaggc gcggccttcc gcgctcgccg ccaagtggcg
      181 ccagctcgtc gacctcgtcg cccagcgccg aagcgaaggc gaggcgcagg ccgaggctct
      241 ggcctggctg aggtcgaacc ggggccgggt cccggcggag gcccggcgcg aggtcgcccg
      301 gagcctcgcc ggccgccggg tcagtccggc cgcgctttcc ttcttcgccg aggatctgcc
      361 cgccgtcgct tcgcctctga tcggcggcgc ccggctcggc gccgacgaat ggctcgacct
      421 gctgccgcac ctcggtcccg tcgcgcgggg cctgctgcgt caccggcgcg acctgcctcc
      481 ggacgtcgtc catgccctgg cccgcttcgg cgccggcgat ttcgcgctgg gcggcgaagt
      541 cgcagccccg gccgtggaca gcctcagtcc cgccgccgag cgcagcgaag cgcagatccg
      601 cgacctcgtc gcccggatcg agagcttccg ccgccgccgc gacgcggccg gagccgcggc
      661 ggcggccgag ccggcgccgg tcgaggagtt ccagtgggaa accgggcccg acggggtgat
      721 cctctgggtc gaaggcgcgc cgcgcgggcc tctggtcggc cacagcatcg cctcgatcgc
      781 cgagcggggc caatatggag tcgacggcca ggccgccggc gccttcgaga aaagggcgcc
      841 gttccgcgac gcccgcttca gcgtccccgg cggcggcgcc gcctcgggcg actggcggat
      901 ttcgggagtg cccttcttcg acgccgcgca gggcaatttc ctcggctatc gcggcagcgc
      961 ccgtcgtccc cggctcgacg aggtcgccca tggcgccgcg cccgcggaag cgccggccgg
     1021 cctgttcgga accgaccttc ccgccgattc gctccgccag ctcatccacg agcttcgaac
     1081 gcccctcaat gcgatcatcg gcttcgccga gatgatcgac ggccaatata tgggccccgc
     1141 cgccttgagc taccgttcgc gcgcgtccga catcatggac caggccaatc gcctgctctc
     1201 ggccgtcgac gatctcgaca ccgccgcccg gctcgagacg cagcgcctcc atctctcgga
     1261 aagctcggtc gacgcggtcg ccttgctctg ccggctccac gacgcttacg aaagggtcgc
     1321 ccgccagcgc ggcagccgga tcctcatcga gatcgagcgc gacctgcccg acgccaaggt
     1381 cgaactggac gccgccgagc gcatgttcgc ccggatgctc gccgccacca tcggcctcgc
     1441 cagggaagcc gagacgatca ccgccaccat ggcgatgggc gaggaagggg cgcgcaagat
     1501 gctctgcctg tcgatcgacc ggcccagggc gatcgagggg ctggacgaaa acgcccttct
     1561 cgacccgggc tacagccccg acggcgactg gcccggcgcc ccggccctcg gcctcggctt
     1621 cgccttgagg ctggtccgca acctcgccga ggcggtgggc ggcgccctcg agatcggcac
     1681 cggccgcttc ttcctctatc tgccgccgct cgagccggcg gaacgctcgt ccggccagaa
     1741 cggttagggg ttggtggcgc ccgacgacga acccgacgcg cggaccggcc gcctcgaccg
     1801 gccgccgccg gagagctcct acatcgcgct tccgcgaagc ttcggccgcc gtttcaccgt
     1861 cttttgcgac accgaggagg agttcgactg ggcgaagccg cgaagccgcg acaatcgctc
     1921 caccacccac atggccgcca tgccggacct gcaggagcgc tttcggcggc gcggtgtgaa
     1981 atgcgtccat ctcgtcgacc atccgatcgc caccgacccc gcctcggtcg caatgcttcg
     2041 cgggtggctc gagcgggacg agatttcggt cggcacccag cttcatccct gggtgaaccc
     2101 accccatgag gaggaagtga acactttcaa cagtttcgtc ggcaatcttc cgatctctct
     2161 cgaacgcgcc aagatcgaga acctgaccgc gacgatcgaa tccgccttcg gccgccgccc
     2221 gatcgtctac cgggccggac gttacggagt cggccccaac accgccgtgt tgctcagcga
     2281 gtcgggctat gaggcggacg tctcggtgcg cgcgctgcac gattatagcg acgagcgcgg
     2341 ccccgatttc acccgggtga agcccttccc gttccgaatc ggcggcgacg gcctcgtcga
     2401 actgccgctc agcgccgctt atgtcgggcc gctgcgctcc agaggcaggt cgatctaccg
     2461 cgccgccgga cgtttgccgc gcgggcgcgg ccttctcttc cgaaccggac tactgaaccg
     2521 cgcggcgctg actccggaag gggttccgct cgaggaagcc ctggccgcgg ctcgggcgct
     2581 gatcgaggac ggcgttagct ggttcagcct gtccttccat tcgccctcgg tcgagccggg
     2641 ccacactccg ttcgtccgga ccgccgagga cctgcgcacc ttccatgcct ggtgggacgg
     2701 gatgttcgac ttcttcgaac gcgaggggat agccccggcc tcgatcgagg agacgatcgc
     2761 ggcggccaaa gcggcctgac caggcgcctt gccttcgccg cccccgctgc ctatccggca
     2821 aaaggctgga gttacgggcc tgtagcgcaa tggtagagcc ggccgctcat aacggctagg
     2881 ttgcgggttc gaatcctgcc gggcccacca ccgctcccgg gccgtttcag gagcggccga
     2941 gcagtggcgc ctcgaggctg agcggcactc cggcctgggc ggccaccgcc tccgcccatt
     3001 ccaccgcttc cccgatttcg gcgccgtagg cgaagccggc gagggcttcc tcgcgcttaa
     3061 gggctgccgg atcgaagctt cggccgggcc gcttggaatg ctccccgaac accgatccgg
     3121 cggcgatcgc cgccgcctgc tcctccgtca gcccgagccc gaaatgcgcc gacgccgcgt
     3181 gcaccgtccg cgccggctcg gccaggaagg aatccgcccg gagagtcgcc acccgaccct
     3241 ccggaagcgt ggctgcgagt cggacgaacc gggcctgatg gttaagccag acgaacgccg
     3301 ccacctcgag atcggtgagc agcagcagat cgttcgtccg cttcgtctcg aactggggat
     3361 ggcgccggta gatcgtcgcc agcgagcgcg cccaggaacg atatttccgg ccccgccggg
     3421 cgatggtcag aaggaaggct ggcagagccg catacatgag caggatgcgg gatccgggcc
     3481 gaaacgccag aatcgcgtcg atcagcggat tggcgaagtc gctgggcttg atcaccgtgg
     3541 tcccccccac ctgatggggt cgttcgagca ggtcgaggac gactcggagg gcctgctgct
     3601 gatcgtcggg ccagtgcgat ttcggcagca gacaggcaag gtcggtcagc gcttgcggtt
     3661 cgtgcaggga cgtggccgcg cccgcttcac cgagggcccg gctcatcagg gtggagcagc
     3721 agaaagcgga gtggaagatg aagtggcaac cagccgccgt cggcggcttg tccttcccgg
     3781 cgagctcggc gacggccagc tcgaaccggg gaatctcctc cgccacgaag cggtggtcga
     3841 ggaaggaaag cccgcgctgc tcgtcccgcg gcaggtggac cagctgcagg gttcggccct
     3901 cgtcgagcac ccggtggggc agccaggcgg gatcggccag aatggccgcc cgttctttgt
     3961 tgcccgcctc gccggaactg tccatttcgg cggcatagac gagacttgcc gccgccgcta
     4021 cgcggtgcgg ccgctacggg cttgccgcgc cggggcggca aacctaaagg cgatcggggt
     4081 cacggggagt agcgcagcct ggtagcgcat ctgctttggg agcagagggt cgcaggttcg
     4141 aatcctgtct ccccgaccag cctcgtctca ggggcgacga aaaggctggc gaatcggacc
     4201 gatcggggtt tgagaaacca actcgccggc cctatcgttc gccaggccat gaaacgcccg
     4261 atcctcatcc tgctcgcgtt gctcgccgtc gcggtcgcgg cgacgacgct ctatctgggc
     4321 cggcgcgatc gcggccacgc agccgctcaa cccgcgcccc cgtccggccc gagcggcgag
     4381 atgatgggcg ccatgcaccg gatgcacgaa gcggcgaaca agaccgcgat gaccggcgac
     4441 atcgaccggg atttcgtcgc tctcatggtt ccgcaccatc gatcggcggt cgagatggcg
     4501 cgggtctatc tcgagcatgg cagggatccc gcgcttcgcc gcctctccga gaacgtcgtc
     4561 gcctcgcagg aggccgagat ccgccagatg agcggccgcg ccgccgctcc ggcgcccggc
     4621 ccggttcccg gccattagcc gatccgcggc tccagcacgg agtcgagcac gcccgacagg
     4681 gtctccagcc gatagggctt gaacacgacc ggccgtcccg ctccgcccga ccggctgatc
     4741 tcgtcgctat agccggtggt gaggacgacc gggacgttgg ggaggaccgg cgccagcgcc
     4801 tcggcgagct cgatcccgct cattccaggc atgacgacgt cggtgaagac cgcgtcgaaa
     4861 ccgcccgccc gggcgagatc ca
//
LOCUS       DASWNU010000614         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_412742, whole genome shotgun sequence.
ACCESSION   DASWNU010000614 DASWNU010000000
VERSION     DASWNU010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412742"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1831)
                     /locus_tag="VGB57_12265"
     CDS             complement(95..1831)
                     /locus_tag="VGB57_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEY0132166.1"
                     /translation="MPETLVPTPTADILPRRFADFDRLGDALDYAAQGQRGLNFHDPR
                     GVLVRPYPYRELREDALAAAWRLIAAGVKPEDRIAIVAETSPEFAALFFGAVYAGAWP
                     VPLPLPTSFGGRDSYIDQLKVQLASADPTHLFYPAELGAMAGAAAEAAGVKGESWESF
                     GTREGPEAELPRAAPDAIAYLQYSSGSTRFPHGVAVTHRGLLNNLAAHSHGMEVIETD
                     RCISWLPWYHDMGLVGCLLSPMANQISTDYLKTEDFARRPLAWLDLITRNTGTTGSYS
                     PTFGYDICARRVSSLTNVAERFDLSRWRIAGNGADMIRPDVMQAFVDAFAPAGFDAKS
                     FLPSYGLAEATLAVTIMPPGEGIVVELVEETTLSGDGKMHRDRPQRYRAIVNCGRPAR
                     DMEIEIRDEDGATLGNREIGKVHCRGPSVMVGYFRDEEATAACLDSAGWLDTGDMGYL
                     SDGYLYIVGRAKDMIIINGKNHWPQDIEWAVEQLPGFKAGDIAAFSITTPGGEETPAV
                     LVQCRTSDAGERARLRDIIRERVRAITGMNCVIELVPPRTLPRTSSGKLSRAKARSLY
                     LTGEIQPYDIAA"
     gene            1935..2471
                     /locus_tag="VGB57_12270"
     CDS             1935..2471
                     /locus_tag="VGB57_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEY0132167.1"
                     /translation="MRSRPDKRPPPPLDPEGLERLGLFYAGRYATTRAKLAAYLRRKL
                     KERGWSGSGEPPVERLVERFAELGYVDDRGVASARSASLLRRGYGERRVQEALRGAGI
                     AEEDSAEARDISGEDALAAAHRFARRRRLGPYAPTVPDRAARDKAAAAMLRAGHRLDL
                     VRQVLAAGPEDLPEPDVP"
     gene            2547..3146
                     /locus_tag="VGB57_12275"
     CDS             2547..3146
                     /locus_tag="VGB57_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY0132168.1"
                     /translation="MTSRRGLTAEDGAQDPDGADEPPRPEDETLIKLTGTMKWFDATR
                     GFGFLVSEQAKGDILVHFSVLKEHDRRSLPEGAVVECLVAEQERGLQARKVLSIDLSQ
                     AVMPEPQRPSGASSAERIDPSALLDQAGEFEAVRVKWFNRLKGYGFLVREVEDGEDIF
                     VHMETVRRGGLTDLNPDQPLRARIAAGRKGPLAVEVEPN"
     gene            complement(3295..4374)
                     /gene="folP"
                     /locus_tag="VGB57_12280"
     CDS             complement(3295..4374)
                     /gene="folP"
                     /locus_tag="VGB57_12280"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0132169.1"
                     /translation="MSLRHYLRPTAFVDSPFGHDGKVARLAGGMLWFAAVERVTMDEN
                     RLVSSELVPVERVEELLALGSDAERAWRNLTSPRAPLRLRERVIRLDQPQVAGILNVT
                     PDSFSDGGRFDGGEAAAQAGFAMAEAGAAIIDVGGESTRPGARPVWEGDEIERVLPAV
                     KRLAAGGTALSIDTRKGAVMSAALGAGAAMVNDVSALTYDETAAGVVAGAGCPVVLVH
                     HQGAPETMQQAPSYQKPVLFAVYDWLEARIAAAEAAGIARALIAVDPGLGFGKTVQHN
                     LDLMNGLALLHGLGCPIMLGASRKRMIGALSGEAPADARLPGSLALALKAVDQGAQIL
                     RVHDVPETVQALRVWRGLRDAALTP"
BASE COUNT          773 a         1616 c         1679 g          742 t
ORIGIN      
        1 gacggcgcct gccgtcatcc cggcgaaagc cgggatcccg ggacgagaag tctcggcggg
       61 tcaccccgag gtcccggctt tcgccgggac gacgtcaggc cgcgatgtcg tagggctgga
      121 tttcgccggt gagatacagg cttcgcgcct tcgccctgga aagcttgccg gagctggtgc
      181 gcggcaaagt gcgcggcggg accagctcga tgacgcagtt cattccggtg atcgccctca
      241 cccgctcgcg gatgatgtcg cgaagccggg cgcgctcgcc ggcgtcggag gtacggcact
      301 ggacgagcac cgcgggcgtc tcctcgccgc ccggagtggt gatcgagaaa gcggcgatgt
      361 cgcccgcctt gaagcccgga agctgctcca ccgcccattc gatgtcctgg ggccagtggt
      421 tcttgccgtt gatgatgatc atgtccttgg cgcggccgac gatgtagaga tagccgtccg
      481 acagatagcc catgtcgccg gtgtcgagcc aacccgcgga atccaggcag gcggcggtcg
      541 cctcctcgtc gcggaaatag ccgaccatga ccgaagggcc tcggcaatgg accttgccga
      601 tctcgcgatt gccgagcgtc gctccgtcct cgtcgcggat ctcgatctcc atgtcgcgcg
      661 ccggcctgcc gcaattgacg atcgcccggt agcgctgcgg gcggtcgcgg tgcatcttgc
      721 cgtcgccgga gagcgtcgtc tcctcgacca gctcgacgac gatcccctcg cccggcggca
      781 tgatcgtcac cgccagcgtc gcctcggcga ggccgtagct gggaaggaag ctcttggcgt
      841 cgaagccggc cggcgcgaag gcgtcgacga aggcctgcat gacgtcgggc cggatcatgt
      901 cggcgccgtt gccggcgatc cgccagcgcg acaggtcgaa gcgctcggcg acgttggtca
      961 ggctggacac gcggcgggcg cagatgtcgt agccgaaggt cggcgaatag cttccggtgg
     1021 tgccggtgtt gcgggtgatg aggtcgagcc aggcgagcgg gcgccgggcg aaatcctcgg
     1081 tcttcagata atcggtcgag atctggttcg ccatcggcga cagcaggcag ccgaccagcc
     1141 ccatgtcgtg ataccagggc agccaggaga tgcagcgatc ggtctcgatc acctccatgc
     1201 cgtggctgtg cgcggcgagg ttgttgagga ggccgcgatg ggtgaccgcg accccgtgcg
     1261 ggaagcgggt cgagccgctc gaatattgga gataggcgat ggcgtccgga gcggcccggg
     1321 gaagctcggc ctccggcccc tcgcgtgtcc cgaagctctc ccaggattcg cccttgacgc
     1381 ccgccgcctc cgccgccgct ccggccatgg cgccgagctc ggccggatag aagagatggg
     1441 tcggatcggc gctggccagc tgcaccttca attggtcgat ataggaatcg cggccgccga
     1501 acgaggtggg cagcggcagc ggcaccggcc aggcgcccgc atagacggcg ccgaagaaca
     1561 aggccgcgaa ttccgggctg gtctcggcga cgatcgcgat ccggtcctcg ggcttcaccc
     1621 ccgcggcgat caggcgccag gccgcggcga gggcatcctc gcgcagctcc cgatagggat
     1681 aggggcggac cagcaccccg cgcggatcgt ggaagttgag gccgcgctgc ccctgcgccg
     1741 cataatcgag cgcgtctccg agcctgtcga agtcggcgaa tcggcgcggc aatatgtccg
     1801 cagtcggcgt cgggaccaaa gtctcgggca cgggtgtagc ggatcctctc cggtggttac
     1861 gcgcgaactt cctcgcccgg gacgggagag gtacgcattt ataatcggga ccgactgtgg
     1921 cacaatcatg gcgcgtgaga tccagacccg acaaaaggcc cccaccgccc ctcgatccgg
     1981 aggggctcga gcggctcgga ctcttctatg ccggccgcta tgccacgacc cgggcgaagc
     2041 tcgccgccta tcttcgccgc aagctgaagg agcggggctg gagcggctcc ggggagccgc
     2101 cggtggagcg gctcgtcgag cgcttcgccg agctcggcta tgtcgacgac cgcggcgtcg
     2161 cctcggcgcg gagcgcgtcc ctgcttcgcc gcggctatgg cgagcggcgg gtccaggaag
     2221 cgctgcgcgg ggcgggcatc gcggaggagg attccgccga ggcccgggac atttcggggg
     2281 aggatgcgct ggccgccgca caccgtttcg cccggcgccg gcgcctcggc ccctatgctc
     2341 cgaccgtccc ggatcgggcc gcccgggaca aagcggcggc cgcgatgttg cgcgccggcc
     2401 atcgcctcga tctggtccgc caggtgcttg cggcgggccc ggaagacctg ccggagcccg
     2461 atgtcccata aaaacagccg taagcggttc acaaatcctc ggaaggcgtg atagtcaccg
     2521 gggtttgcaa ggtggatcgg ccgttaatga cttcgcgccg tggattaact gctgaagacg
     2581 gcgcgcagga ccccgacggc gcggacgaac ccccgcgccc cgaggacgag accctcatca
     2641 agctgaccgg cacgatgaaa tggttcgacg ccacgcgtgg cttcggcttc ctggtcagcg
     2701 agcaggcgaa gggcgacatc ctcgtccatt tcagcgtctt gaaggagcat gaccgccgaa
     2761 gccttcccga aggcgccgtg gtcgaatgcc tcgtcgcgga gcaggaacgc ggcctccagg
     2821 cccgcaaggt cctgtcgatc gacctcagcc aggcggtgat gccggagccg caacggccct
     2881 ccggggcgag ttcggccgaa cggatcgatc cttccgcctt gctcgaccag gcgggcgagt
     2941 tcgaggccgt gcgggtgaaa tggttcaacc gcctcaaggg ctacggcttc ctcgtccgcg
     3001 aagtggagga cggcgaggac atcttcgtcc acatggagac ggtccgccgc ggcggcctga
     3061 ccgacctcaa tcccgaccag cccctccgcg cccgaatcgc cgccggccgc aaagggccgc
     3121 tggcggtcga ggtggagccg aactgatatc cctctcaaat cctccccggc ttttgccggg
     3181 gagggggacc gcgcgaagcg cggtggaggg gttcttcgga gtcggaaaga acccctccac
     3241 catgcttcgc atggtccccc tcccccggaa atccggggga ggatttttag acgatcaggg
     3301 cgtcagcgcc gcgtccctca ggccgcgcca gaccctcagc gcctggaccg tctccggcac
     3361 gtcgtggacc cggaggatct gagccccctg atccaccgcc ttcagcgcga gggcgagcga
     3421 gccggggagg cgcgcatcgg ccggggcctc tccggaaaga gcaccgatca tccgcttgcg
     3481 gctcgctccg agcatgatcg ggcaaccgag gccgtgcagc aaggcgaggc cgttcatcag
     3541 gtcgagattg tgctgaacgg tcttgccgaa gccgaggccg ggatcgaccg cgatcagggc
     3601 acgcgcgatt ccggccgctt cggccgccgc gatccgcgcc tcgagccagt cgtagacggc
     3661 gaacagcacc ggcttttgat aggaaggtgc ctgctgcatc gtctccggcg ccccctgatg
     3721 gtggaccaga acgaccgggc agccggcgcc ggcgaccacg ccggcggcgg tctcgtcata
     3781 ggtcaaggcc gacacgtcgt tgaccatcgc cgcgcccgcg ccgagcgcgg cgctcatcac
     3841 ggcccccttg cgggtatcga tcgacagcgc ggtcccgccg gcggcgagcc gcttgacggc
     3901 cggaagcacc cgctcgatct cgtcgccttc ccagacgggc ctggccccgg gccgggtcga
     3961 ttcgccgccg acgtcgatga tcgctgcgcc ggcttcggcc atggcgaacc cggcctgcgc
     4021 cgccgcttcc ccgccgtcga accggccgcc gtccgaaaag ctgtccgggg tgacgttcag
     4081 gattcccgcc acctgcggct gatcgagcct gatcacgcgc tcgcgaagcc ggagcggcgc
     4141 gcgcggcgag gtcaggttcc gccaggcccg ctcggcgtcg cttccgagcg caagcaattc
     4201 ctccacccgt tcgaccggga cgagctcgga agagacaagg cgattttcgt ccatcgtcac
     4261 ccgctcgacc gcggcgaacc agagcattcc gccggcgagg cgggccactt tgccgtcatg
     4321 gccgaacggc gagtcgacga aggcggttgg ccgcagataa tggcgaaggc tcatggcgat
     4381 tcggctagcg cataatctcg ccgacaggaa tcccgaagct ggaggactaa ggggccgaag
     4441 ctggtcgtgc ccccgtcatc ccggcgaagg ccgggacctc aggacaagaa gtcccggtag
     4501 gtctccccgc ggccccggct ttcgccgggg tgacggaaaa ggtgacgacc gcctctgatc
     4561 gaaggtcagt agggtaaccg agtctccgag gcccgggcca ccatgtgggg catggtattg
     4621 gtccagaccc atcagcactc aaaggggctc ccgcgcgtct cccgtggtca aaggcgtgcg
     4681 ggcgttgagc gtgaagggac ggtcgtcatg cccaatgtgc ctttgtttcg cgctcatgtc
     4741 gagtggcgga gtgcaacctt ttttgctcga atttgggtca gtccagggtc agcaggtagc
     4801 gctgccggag
//
LOCUS       DASWNU010000615         5872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_23569, whole genome shotgun sequence.
ACCESSION   DASWNU010000615 DASWNU010000000
VERSION     DASWNU010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23569"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGB57_12285"
     CDS             <1..214
                     /locus_tag="VGB57_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132170.1"
                     /translation="GPRGPGRMKMRNLETCLTGLLWFAVALLLPMAALEPVSVASVRA
                     ATSGLAAATCDDGSAHLAMGCASILL"
     gene            345..1655
                     /locus_tag="VGB57_12290"
     CDS             345..1655
                     /locus_tag="VGB57_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0132171.1"
                     /translation="MSVIRIKSTDGEARAESGTGMDKVVASTGLSQRMKIAIGAAALM
                     LFALLFWWFAPSAGSQTVAAERLTVSAVSKGTFDDFLPLRARVEPLVTVYLDAVEGGR
                     VEKIMVEDGAIVQQGQLLAVLSNSDLALNLLARQSDVSREVNSMRSQELALAQTSIGD
                     ERAVIDAQLAADKARRQYEMQKPLMEKGYVPAKVYNDSRDEYLSSRRRAEVLRRARAV
                     NQRLQTSQLAQLRASNASLSGSLDIARSTLDALNLRAPVTGQLTAFSIQVGQSLNRGE
                     RLGQVDSAGRNKLVASVDEFYLGRVQPGQTATVDLGGKTYRAKVAKIYPQVKNGVFEA
                     DLTFVGAEPPGLNRGQTLQTKLTLGDPAPALLIPNGAFYNETGGAWVFVVEPDGGGAV
                     KRNVRLGRRNADYIEVLEGLEPGERVITSPYTGFADKDRLDLET"
     gene            1673..2353
                     /locus_tag="VGB57_12295"
     CDS             1673..2353
                     /locus_tag="VGB57_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0132172.1"
                     /translation="MLTMRELSRVYRADTVETTALDRIDLDIADGEFVAIMGPSGCGK
                     STLLNVIGMLDSPSSGSYVFDGLEVAGLPEAKLANVRKQNIGFIFQSFNLVDELSVRE
                     NIELALLYHDVPASERRSRVEAVMDKVGVAHRAKHRPSQLSGGQQQRVAVARALVGNP
                     KLILADEPTGNLDTQHGEEVMKMLQALNREGSTIVMVTHSPAHADYAGRVVNMLDGRI
                     LIERRRAA"
     gene            2497..2988
                     /locus_tag="VGB57_12300"
     CDS             2497..2988
                     /locus_tag="VGB57_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY0132173.1"
                     /translation="MRSRLLLILLAAAVTLAASAAQAGERQRSTVLMSENAQGRKFQE
                     EWGYSDAIVAGDTIYLSGIVVGQQGGESLEAAYERTYDQIGAILKRAGASWDDVVDIT
                     SFHTDVVAQMPAIVAAHKRRVKAPYPAWTAIDVDRLIPERGITEIKIVAKRRAAATAG
                     RSR"
     gene            3015..5516
                     /locus_tag="VGB57_12305"
     CDS             3015..5516
                     /locus_tag="VGB57_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0132174.1"
                     /translation="MLKMWQNYLTVALRALGRHKVYAFINIFGLALGLAACLLILLYV
                     RYETSYDRSLPDAEQVYQVQTIHTDPETGNRRQMQATEGVVTESLAKDFPQIEAIARA
                     EGDRPVLMDRGEARYAEVLSADPTFFQILQVPFLGGNPKTALRDADSLVISRSEALKR
                     FGTIDAVGRTVTGIRRGEKYEMRVTGIFEDFARNSHLGFTLVSRIREEEKKDCGWGCL
                     NGVVYLKLRPGASADDVNRQLPAWEMRNIPRKNVSGQMVGEGDAFDWRLVRLPEVHLS
                     GAEAFWEERPGNDRRSIVTFAIVALLILGMACVNFINLATARAGQRAREVALRKVLGA
                     SRRQLVTQFLGESLLLTAVAMLIALAMAELVLPWLADFLRADLKIDYWGPQGILVAAL
                     ALLLVVGIAGGLYPALYLSRYQPGRVLKANQSTAEPAGTGRLRNMLVVAQFAVSIGLI
                     ACTLVVYGQTRFAQSADPGYRREGLIQATNLNRAAVIPLTESMIREVGKVPGVTAVAA
                     ANIRVASGQTLNTRIELPGRTKSLTIGWYSVHPGFFDTMGIRLVAGRRLSRQYANDSA
                     YTPWETEEAATAGARALVARGINVVVNELAAKQMGFASPAEAVGKQIKARAFADEVGT
                     LPVTIVGVVQDSRFRSARDPVEPMIFPDEGIYSSLIVRYDSADPQGVRAGVERVWKRL
                     ASGVPFEGDFADAQLAELYLADQARGTTFAGFAFLAVVIASLGLFGLAAFTAERRTKE
                     IGIRKVFGARIRDIVQLLAWQFSKPVVLANLIAWPAAWWVMRDWLNGFDARIDLGPGP
                     FLMAGFLALAIALGTIAGHAVKVARLNPIHALRYE"
     gene            5527..>5872
                     /locus_tag="VGB57_12310"
     CDS             5527..>5872
                     /locus_tag="VGB57_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0132175.1"
                     /translation="MLRNYLTVAMRSLTRNRAYALINICGLALGLAACLTILLYVRYE
                     TSYDSWLPDHERIHQVQATWHESGQPVSKSQHSPFPVRDTLAAGFPQIEAVTVLRSGK
                     TVLMHKGQPMFLD"
BASE COUNT          988 a         2007 c         1950 g          927 t
ORIGIN      
        1 tgggccgcgg ggcccgggga gaatgaagat gcgcaatctg gaaacctgcc tgaccggcct
       61 gctctggttc gccgtggcgc tgctgctgcc gatggcggcg ctggagccgg tgagcgtcgc
      121 cagcgtccgg gcggccacga gcggcctcgc cgccgcgacc tgcgacgacg gcagcgccca
      181 tctcgccatg ggttgcgcga gcatcctgct gtgaccttgt ccgtccgcgt acaggcctgt
      241 gtccggaatc ggacgttggc gggacgcggc gggctgaaag agtgcctgcg cggtcgcccc
      301 gtccgtggca cgatcgttgc tgaacggacg ggcatgaggt tgggatgagc gtgatcagga
      361 tcaaatccac ggacggcgag gctcgggcgg aaagcggcac cggaatggac aaggtggtgg
      421 cctccaccgg tctgtcgcag cgcatgaaga tcgccatcgg cgcggccgcc ctgatgcttt
      481 tcgccttgct cttctggtgg ttcgcgccga gcgcgggcag tcagacggtg gcggccgagc
      541 ggctgacggt gtcggcggtc agcaagggca cgttcgacga tttcctgccc ttgagggctc
      601 gggtcgagcc cctggtcacc gtctatctcg acgccgtcga gggcggacgg gtcgagaaga
      661 tcatggtcga ggacggcgcc atcgtccagc agggccagct gctcgccgtg ctgtccaact
      721 cggacctcgc gctcaacctg ctggcgcgcc agtcggacgt ctcgcgcgag gtcaactcga
      781 tgcgcagcca ggagctcgcg ctcgcccaga ccagcatcgg ggacgagcgc gccgtcatcg
      841 acgcccagct cgccgccgac aaggcgaggc gacagtatga gatgcagaag ccgctgatgg
      901 agaagggcta cgtacccgcg aaagtgtata acgacagccg cgacgaatat ctctccagcc
      961 ggcgccgcgc cgaggtcctc aggcgcgccc gggcggtcaa ccagaggctc cagacgagcc
     1021 agctcgccca gctccgagcg tccaacgcct cgctgagcgg aagtctcgac atcgcccgct
     1081 cgaccctcga cgctctcaat ctgcgcgctc cagtcaccgg ccagctcacc gctttctcga
     1141 tccaggtcgg ccagtcgctg aaccggggcg agcggctcgg ccaggtcgac agcgccggcc
     1201 gcaacaagct cgtcgcctcg gtggacgaat tttacctggg cagggtccag ccgggccaga
     1261 cggcaaccgt cgatctgggc ggcaagacct accgggccaa ggtcgccaag atctatccgc
     1321 aggtcaagaa cggcgtcttc gaggcggacc tcaccttcgt cggcgccgag ccgccggggc
     1381 tcaatcgcgg ccagaccctg cagaccaagc tgacgctcgg cgatcccgcc ccggccctcc
     1441 tcatccccaa cggcgccttc tacaacgaga ccggcggagc ctgggtgttc gtggtcgagc
     1501 ccgacggcgg cggcgcggtc aagcgcaacg tccgcctggg ccgccgcaac gccgattata
     1561 tcgaggtgct cgaggggctg gagcccggcg agcgagtgat cacctcaccc tataccggat
     1621 tcgccgacaa ggaccggctc gacctcgaga cctgacgaag gagcttcaag caatgctgac
     1681 catgcgtgaa ctgtcgcggg tctaccgcgc cgataccgtc gagacgacgg cgctcgaccg
     1741 gatcgacctc gacatcgccg acggcgagtt cgtcgcgatc atgggaccgt cgggctgcgg
     1801 caagtcgacc ctcctgaacg tgatcgggat gctcgattcg ccgagcagcg gctcctacgt
     1861 cttcgacggc ctggaggtgg cggggcttcc cgaagccaag ctcgccaacg tgcgcaagca
     1921 gaatatcggc ttcatcttcc agagcttcaa tctggtcgac gaactctccg tccgcgagaa
     1981 tatcgagctc gccctgctct accacgacgt ccccgcctcc gagcggcgct cgcgggtcga
     2041 ggcggtgatg gacaaggtcg gggtcgccca ccgcgccaag caccggccga gccagctttc
     2101 gggcggccag cagcagcgcg tcgccgtcgc ccgggcactt gtcggcaacc ccaaattgat
     2161 cctcgccgac gagccgaccg gcaacctcga cacccagcat ggcgaggaag tgatgaagat
     2221 gctccaggct ctcaatcgcg agggctcgac catcgtcatg gtcacccact cgcccgccca
     2281 tgccgattat gccggacggg tggtgaacat gctggacggc cggatcctga tcgagcggcg
     2341 ccgggcggcg tgaggggatt ggctttttta aatcctcccc gggatttctc ggggaggggg
     2401 accgtgcgaa gcacggtgga ggggttcttc ggagtcagca aggcccctcc accgtccttc
     2461 ggacggtccc cctcccctgc aaatgcaggg gaggatttga ggagccggtt actcctcatc
     2521 cttctggccg ccgccgtcac ccttgccgcc tccgccgccc aggccggcga gcgccagcgc
     2581 tcgaccgtgc tgatgagcga gaacgcgcag ggccggaagt tccaggagga atggggctat
     2641 agcgacgcga tcgtcgccgg ggacacgatc tacctgagcg ggatcgtcgt cggccagcag
     2701 ggcggcgagt cgctcgaggc ggcctacgag cggacctacg accagatcgg agcgatattg
     2761 aagcgggccg gggcgagctg ggacgacgtc gtcgacatca ccagcttcca caccgacgtc
     2821 gtcgcccaga tgcccgcgat cgtcgcggcg cataagcgcc gagtcaaagc gccttatccg
     2881 gcctggaccg cgatcgacgt cgaccggctg atcccggagc ggggaatcac cgagatcaag
     2941 atcgtggcga agcggcgcgc cgccgcgacg gccgggcgaa gcagataggc ccggaaaggg
     3001 cgggggaacg atccatgctg aagatgtggc agaactacct gaccgtcgcc ctccgcgccc
     3061 tggggcggca caaggtctac gcgttcatca atatcttcgg actcgcgctc ggtctcgcgg
     3121 cctgcctcct catcctcctc tacgtccgct acgagaccag ctacgaccgc tcgctgcccg
     3181 acgccgaaca ggtctatcag gtgcagacga tccacaccga tcccgagacc ggcaaccggc
     3241 ggcagatgca ggcgaccgag ggagtcgtca ccgaaagcct cgccaaggat ttcccccaga
     3301 tcgaggcgat cgcccgggcg gaaggcgacc ggccggtgct catggaccgc ggcgaagccc
     3361 gatatgccga agtcctgagc gccgacccga cctttttcca gatcctgcaa gtgccgttcc
     3421 tgggcggcaa tccgaagacg gcgctgaggg atgcggacag cctcgtcatc agccgaagcg
     3481 aagccctgaa aaggttcggg acgatcgacg cggtcggccg caccgtcacc ggtattcgcc
     3541 ggggcgagaa atacgaaatg cgggtcaccg gcatattcga ggactttgcg cgcaacagcc
     3601 atctcggatt caccctcgtc agccggatcc gcgaagagga gaagaaggat tgcggctggg
     3661 gctgcctcaa cggtgtcgtc tatctgaagc tgcgcccggg cgcgtccgcc gacgatgtca
     3721 accggcagct tccggcctgg gagatgcgca acatcccgcg caagaacgtc agcggccaga
     3781 tggtcggcga aggcgacgcc ttcgactggc gcctggtgcg actgcccgaa gtccatctga
     3841 gcggcgccga agccttctgg gaagagcgcc cggggaacga ccgccggagc atcgtcacct
     3901 tcgccatcgt cgcccttctg atcctgggaa tggcgtgcgt gaacttcatc aacctggcca
     3961 ccgcccgagc ggggcagcgc gcccgggaag tggcgcttag aaaggttctc ggcgccagcc
     4021 gacggcaatt ggtgacccag ttcctgggcg agtcgctgct tctgacggcg gtggcgatgc
     4081 tgatcgcgct cgccatggcc gagctggtcc tgccctggct ggcggacttc ctgcgcgccg
     4141 acctcaagat cgactattgg gggccgcagg gcatcctggt ggccgctctg gccctgctgc
     4201 tcgtcgtggg catagcgggc ggtctctatc cggccctcta tctctcgcgt taccagccgg
     4261 gccgggtcct gaaggcgaac cagtcgacgg cggaacccgc gggcaccggc cggctgcgca
     4321 atatgctggt ggtggcgcaa ttcgccgtct cgatcggcct tatcgcctgc accctcgtgg
     4381 tctacggcca gacccgcttc gcgcagagcg ccgaccccgg ctatcgccgg gaggggctga
     4441 tccaggccac caacctgaac cgggccgccg tcatcccgct gaccgaatcc atgatccgcg
     4501 aggtcggcaa ggttccgggg gtcaccgccg tcgccgccgc caacatacgg gtcgccagcg
     4561 gccagacgct caatacccgg atcgagctcc ccggccggac gaagtcgctg acgatcggct
     4621 ggtacagcgt tcatccgggc ttcttcgaca ccatgggtat ccgtctggtc gccggccgca
     4681 ggctgtcacg ccaatatgcc aatgacagcg cctacacccc ctgggagacg gaggaggcgg
     4741 cgaccgcggg cgccagggcg ctggtggcgc gcggaattaa cgtcgtggtg aacgagctgg
     4801 cggcgaagca gatgggcttc gccagcccgg ccgaggcggt cggcaagcag atcaaggccc
     4861 gcgcgttcgc ggacgaggtc gggacgctcc ccgtcaccat cgtcggagtc gtccaggatt
     4921 cacgcttccg ttccgcccgc gacccggtcg agccgatgat cttccccgac gagggcatct
     4981 attcgagcct gatcgtccgc tacgattcgg ccgaccccca gggcgttcgc gccggggtcg
     5041 aacgggtgtg gaagcggctc gcctccggcg tacctttcga gggcgatttc gcggacgcgc
     5101 agcttgccga gctatatttg gccgaccagg cgcggggcac gaccttcgcg ggcttcgcct
     5161 tcctcgccgt ggtcatcgcc agcctgggcc ttttcggcct tgccgccttc accgccgagc
     5221 ggcggaccaa ggagatcggc atccgcaagg tgttcggcgc ccggatccgc gacatcgtcc
     5281 agctgctcgc ctggcaattc tcgaagccgg tggtgctggc caacctcatc gcctggccgg
     5341 ccgcctggtg ggtgatgcgc gactggctga acggcttcga cgcgcgcatc gacctcgggc
     5401 cggggccgtt cctgatggcg ggtttcctcg cgctcgccat cgccctcggg accatcgccg
     5461 gccacgcggt gaaggtcgcc cggctcaacc ccatccacgc cctccgctac gagtaggacg
     5521 cagacgatgt tgcgcaatta tctgaccgtg gcgatgagga gcctgactcg gaaccgcgcc
     5581 tacgccctca tcaacatttg cggcctcgcg ctcggcctcg ccgcctgcct gacgatcctg
     5641 ctctacgtcc gctacgagac gagctacgac agctggctcc cggatcacga gcgaatccat
     5701 caggtgcagg cgacctggca cgagtccggg cagcccgtct ccaagtcgca gcacagcccg
     5761 tttcccgtgc gggatacact ggccgccggc ttcccccaga tcgaggcggt cacggtcctg
     5821 cggtcgggca agacggttct gatgcacaag ggccagccga tgttcctcga cg
//
LOCUS       DASWNU010000616         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_464037, whole genome shotgun sequence.
ACCESSION   DASWNU010000616 DASWNU010000000
VERSION     DASWNU010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464037"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VGB57_12315"
     CDS             complement(<1..1216)
                     /locus_tag="VGB57_12315"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0132176.1"
                     /translation="MTNDPIPAFEVEAVAEGEGTSFTVTEVGRETRHYRLPGGEQDDY
                     TAFYKELARDYRMRDTSRHPNPPPVVPGSPEWRPLITQTIDPRIPAGHGDPAVLKTDE
                     GYFLVSTSNDAPNAFPILRSDDLEKWELVGFVFPEGHAPSWTAAGRHVGDFWAPEMAK
                     VGEEYWIAYTAREKSNALAIGIAKSKTPYGPWQDLGYPLITGGVIVCPPTGALAGTEP
                     PKASGGVIDSHFFIDSDGALYLFWKQDTNGVWPRPLAGLMRERPELIAQLFDSEEERR
                     TAAFAAAVQPWANSRRPIERFFLMQPLIEIALAHWPKVRRVLEECGHAGHIVEAMRTP
                     IRAQRLAPDGGSLIGDETIVLANDLDWEGHLIEGPWVTRQQGRYWLFYAGNDFCTPAY
                     GIGVAVADHPLGP"
BASE COUNT          236 a          473 c          572 g          249 t
ORIGIN      
        1 aaggccccag cgggtggtcg gccacggcga ccccgatgcc gtaggccgga gtgcagaagt
       61 cgttgccggc gtagaacagc cagtagcggc cctgctgacg cgtgacccag gggccttcga
      121 tgaggtggcc ttcccagtcg agatcgttgg cgaggacgat cgtctcgtcg ccgatcagcg
      181 agccgccgtc gggggcgagg cgctgggcgc ggatcggggt ccgcatcgcc tcgacgatgt
      241 ggccggcatg gccgcactcc tcgagcacgc gccgcacctt cggccagtga gcgagcgcga
      301 tctcgatcag gggctgcatc aggaagaagc gctcgatcgg acggcggctg ttggcccagg
      361 gctggaccgc ggcggcgaag gcggcggtgc gccgctcctc ctcggagtcg aagagctgag
      421 cgatcagctc gggccgctcg cgcatcaggc cggcgagcgg gcgcggccag acgccgttcg
      481 tgtcctgctt ccagaacagg tagagcgcgc cgtcggagtc gatgaagaag tgggagtcga
      541 tcacgccgcc gctcgccttg ggcggctcgg tgccggcgag ggcgccggtc ggggggcaga
      601 cgatcactcc gccggtgatg agcggatagc cgagatcctg ccacgggccg tagggcgtct
      661 tgctcttggc gatgccgatg gccagggcgt tgctcttctc gcgggcggtg taggcgatcc
      721 aatattcctc gccgaccttg gccatttccg gcgcccagaa gtcgccgacg tggcgcccgg
      781 ccgcggtcca cgaaggggcg tggccttcgg ggaagacgaa gccgaccagc tcccattttt
      841 cgaggtcgtc ggagcgcagg atcgggaagg cgttcggcgc gtcgttcgag gtggagacga
      901 ggaaatagcc ctcgtcggtc ttgagcaccg ccgggtcgcc gtgaccggcg gggatgcgcg
      961 gatcgatcgt ctgcgtgatg agcggtcgcc attcggggga gccgggaacc acgggcggcg
     1021 gattgggatg acggctcgta tcccgcatcc gatagtcgcg cgcgagttcc ttgtagaagg
     1081 cggtgtaatc gtcctgctcg ccgcccggca gtcggtaatg gcgggtttcg cggccgacct
     1141 cggtgacggt gaagctcgtg ccctcgccct cggcgacggc ttcgacctcg aaggcgggaa
     1201 tggggtcgtt cgtcatgcgg cgccgagtta gtgatcgccc gccaaaagag aagccattgt
     1261 ttcgggaccg ctttggcaaa ggcacaaaaa cctagtctgc gccgggcttt agtgcgatag
     1321 tgcgacggca gtgcggggtg gcgttgtgac gcaacgggtg aaccaggaca ggatcaaatc
     1381 ggcggccgcg gtcgccctgt tccacgcgct gatcggctat gccttcatca ccgggcttgg
     1441 gtacgaggcg gcccgaagcg ccggcgagac tctgaagctg ttcgacgtca ggccggaacc
     1501 gcccccgcct ccggtcgagg agcccgcccc
//
LOCUS       DASWNU010000617         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_127583, whole genome shotgun sequence.
ACCESSION   DASWNU010000617 DASWNU010000000
VERSION     DASWNU010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127583"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..250
                     /locus_tag="VGB57_12320"
     CDS             5..250
                     /locus_tag="VGB57_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132177.1"
                     /translation="MLALIIPPLTACTVARTAVDVVSLPVKVVSAGVDAATTSQAEAD
                     QKRGRQIRRQEECMGKEERKARKQDREPDYDRCEPKK"
     gene            complement(389..1045)
                     /locus_tag="VGB57_12325"
     CDS             complement(389..1045)
                     /locus_tag="VGB57_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0132178.1"
                     /translation="MIDLYTAATPNGWKVSILLEELNLPYEVHVLDLRSGAQKEPWFT
                     AINPNGRIPAIVDRDEEDFAVFESGAIMVYLAEKTGRLMPADPKGRSLVMQWLMFQMG
                     GVGPMMGQANVFTRYFPEALPGVIDRYRRESRRLFEVLDGHLANREWLAGDYSIADIA
                     NFAWVRTHEWPGVSVEGLDHLQRWMAAIAERPAVQKGVTIPPRADPDKTVEAARSMLA
                     "
     gene            complement(1151..>2410)
                     /locus_tag="VGB57_12330"
     CDS             complement(1151..>2410)
                     /locus_tag="VGB57_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0132179.1"
                     /translation="REPAFAAGFIGSALGLVLILTLLGGLVRWVASRLPRPANPVFRI
                     ALANLHRPGAQTGRLVVALGLGLTLFTTLAVIESNLAGQIRTTIPARAPSFFMLDIPV
                     EEIDRFRTLARRSAPQGELVTIPSLRGSVVALGGRRVADMTEIPPEAWFLRGDRGLTY
                     SAALPEGSRIVDGRWWPADYAGPPLVSLDVEAARAVGLKVGDSLTISILGREIEARIA
                     SLREINWDTMGFNFVIVFAPGTLESAPHSFMATISMPESQERPFARAVSSAFPSVSAI
                     RVKEVVETVSGMLSQLAVAVRAAASVAILAGNAVLVGALAASRRARTYDSVILKMLGA
                     TRPRILAAQAAEFAALALVVSLVALGLGTGAGWWVVVKIFELDWAPDWGTVAATLALG
                     AVVTLALGLLGSLPALAARPARALRTL"
BASE COUNT          410 a          827 c          830 g          343 t
ORIGIN      
        1 ccttctgctt gccttgatca ttccgcccct cactgcctgc accgttgcgc gcacggcggt
       61 cgacgtggtc agcctgccgg tgaaggtcgt ttccgccggc gtcgatgccg ccaccaccag
      121 ccaggccgaa gccgaccaga agcgcggccg ccagatccgg cgccaggaag aatgcatggg
      181 caaggaggag cgcaaggccc gcaagcagga ccgcgagccc gattacgacc ggtgcgagcc
      241 gaagaagtaa gctaaaatcc tccccgagat ttctcgggga gggggaccgc gcgaagcgcg
      301 gtggaggggt tcttcggagt ccgagtgccc ctccaccagg cttcgcctgg tccccctccc
      361 ctgcaactgc aggggaggat tttgacgatc acgccagcat cgatctcgcc gcttccaccg
      421 tcttgtccgg atccgcccgc ggcgggatgg tcacgccctt ctgcacggcc ggccgctcag
      481 cgatcgccgc catccagcgc tggagatggt cgagtccctc gaccgatacg cccggccatt
      541 cgtgggtccg aacccaggcg aaattggcga tgtcggcgat cgaatagtcg ccggccagcc
      601 attcgcggtt cgccagatgc ccgtccagca cctcgaacag ccgccggctc tcgcggcggt
      661 agcggtcgat gaccccgggc agagcctcgg gaaaatagcg ggtgaagacg ttggcttggc
      721 ccatcatcgg ccccactccg cccatctgga acatcagcca ttgcatgacc aggcttcggc
      781 ccttcggatc ggcgggcatc agccggcccg tcttctcggc cagatagacc atgatcgcgc
      841 ccgattcgaa cacggcgaaa tcctcctcgt cgcggtcgac gatggcggga atccgcccgt
      901 tcgggttgat cgcggtgaac cacggctcct tctgggcgcc ggagcgaagg tcgaggacgt
      961 ggacctcgta gggcaggttc agctcctcga gcaggatcga gaccttccag ccgttggggg
     1021 tagcggcggt atagaggtcg atcatgattt cctccgtcat cccggcgaag gccgggatcc
     1081 atgaacacgg acgtgctcgt gaccgtgagg tcggtgttca tggaccccgg atcaagtccg
     1141 gggtgacggg ctagagcgtt cgaagcgccc gcgccggcct tgccgcgagc gccgggagcg
     1201 acccgagcag ccccagcgcc agagtcacca ccgctccaag cgccagggtc gccgcgaccg
     1261 tcccccaatc gggcgcccaa tccagctcga agatcttcac gaccacccac cagccggccc
     1321 ctgtgcccag cccgagggcg acgaggctga ccacgagcgc cagagcggcg aactcggccg
     1381 cctgggcggc gaggatccgg gggcgggtgg ctccgagcat cttcaggatg acgctgtcgt
     1441 aggtgcgggc ccggcggctc gcggcgagcg ctccgacgag cacggcgttt ccggcgagga
     1501 tcgcgaccga ggcggcggcg cgcaccgcca cggcgagctg cgacagcatc ccgctcaccg
     1561 tctcgaccac ctccttgacc cggatcgccg agacgctggg aaaggcgctc gatacggctc
     1621 gggcgaaggg gcgctcctgc gactccggca tcgagatcgt cgccatgaag ctgtgcgggg
     1681 cgctctccag cgttccgggg gcgaagacga tgacgaagtt gaagcccatc gtgtcccagt
     1741 tgatctcgcg gagcgaagcg atccgggcct cgatctcacg gcccaggatc gagatggtga
     1801 ggctgtctcc gaccttcagc ccgaccgccc gggccgcttc gacgtcgagc gagacgaggg
     1861 gcggcccggc ataatccgcc ggccaccagc gcccgtcgac gatccggctc ccctccggca
     1921 gggccgcgga ataggtcagc ccgcggtcgc cgcgcaggaa ccaggcttcg ggcggtattt
     1981 cggtcatgtc cgcgacccgc cggccgccga gggcgacgac cgaaccgcgc agcgagggga
     2041 tggtgacgag ctcgccctgc ggcgccgagc ggcgggccag ggtgcggaag cggtctatct
     2101 cctccaccgg aatgtcgagc atgaagaagc tcggcgccct ggcggggatg gtggtccgga
     2161 tctggccggc gagattgctc tcgatcaccg ccagggtcgt gaacaaggtt aggccgaggc
     2221 cgagggcgac caccagccgg ccggtctggg cgcccggacg atggaggttg gcgagcgcga
     2281 tcctgaagac gggattggcc ggcctcggca gccgcgaggc gacccaacgg accagcccgc
     2341 cgagcagggt gagaatcagg acgagaccga gcgccgagcc gatgaagccc gccgcaaaag
     2401 cgggctcccg
//
LOCUS       DASWNU010000618         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_101224, whole genome shotgun sequence.
ACCESSION   DASWNU010000618 DASWNU010000000
VERSION     DASWNU010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101224"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..943
                     /locus_tag="VGB57_12335"
     CDS             86..943
                     /locus_tag="VGB57_12335"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0132180.1"
                     /translation="MPTPIVQLFYNRTSGGHCPRRLAALQAGFEAKGAKVLLSECGPA
                     NPIEVGEETSHVCAVGGDGTLRHVALALARCGRRLPLSVYPAGTVNLVHRERLFPLDP
                     EGHAERLLGESDGVFHPAALNDTFFLACASVGPDSRAVAGVSLALKARIGKLAYGWAL
                     VRLLLRWPRAPIRLAWDGGALVCEAFYVAKGRYFAGPWSFAPEADIARPLLHVVALAR
                     ARRRDFARFAFALWRGRRVDALPGITAFTCTALEAEADVPLPIQADGDPVGTLPASIS
                     LHGEALSLC"
     gene            complement(1113..2093)
                     /gene="glpX"
                     /locus_tag="VGB57_12340"
     CDS             complement(1113..2093)
                     /gene="glpX"
                     /locus_tag="VGB57_12340"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0132181.1"
                     /translation="MVSASHVLDRVLVLEMVRVTEAAAVAASKLIGRGDEKAADHAAV
                     EAMRAALNTLEMDGTVVIGEGERDEAPMLYIGEKVGAAQGSGPRIDIALDPLEGTTIT
                     AKGAPNALAVLAIAEEGGLLNAPDVYMEKLAVGPGYAEGVIDLNRSATENIRSVAAAK
                     GVDPSEIIVCVLDRERHQALIAELRGLGCGIVLIPDGDVAGVIAVTDPDTTIDVYMGS
                     GGAPEGVLAAAALRCVGGQFQGRLIFRNDDERARADKWGVTDLDRVYKLEDLAKGDCI
                     FAATGVTDGSLLEGVKRRKNAITTESVVMRASTGTVRWVRGEHRHEPGAR"
     gene            2342..2734
                     /locus_tag="VGB57_12345"
     CDS             2342..2734
                     /locus_tag="VGB57_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132182.1"
                     /translation="MAKLSLAVAAAVLASAPAASAPPPSADEALARQQGSLRSSLGLD
                     CDRDSGEIVVCGRTGPDPDRLPLPVPPEPGARRSGEPVDPSDTLALSAETCTAVGRNP
                     RCSGGLPILGIAAMIVQTVVKAAIKDEE"
     gene            2731..3126
                     /locus_tag="VGB57_12350"
     CDS             2731..3126
                     /locus_tag="VGB57_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132183.1"
                     /translation="MRGRSAAFALLASAAAPAFALAPEAPVTAERAMEIQKDSVRDAV
                     GTLPCRRDGAGGDIIVCGRRGPDPNRVPFPDQRTPGEPENVLPGELARAQPSFGTCEF
                     GCQQPVTITDKDVRKYGGRILRHILGKDD"
BASE COUNT          445 a         1257 c         1032 g          491 t
ORIGIN      
        1 acccgcagac ttctcgacct gaggtcccgg cttccgccgg gatgacgcta attggacttt
       61 aggggggtcg tgctacaccg ccccgatgcc cactccgatc gttcagctct tctacaaccg
      121 cacctccggc ggccactgcc cgcgccggct cgccgccctt caggccggtt tcgaggcgaa
      181 gggagcgaag gtcctgctct ccgaatgcgg cccggccaat ccgatcgagg tcggggagga
      241 gaccagccac gtctgcgcgg tcggcggcga cggcaccctt cgccacgtcg ccctggcgct
      301 ggcgcgttgc ggacgaaggc ttccgctcag cgtctatccc gccggcaccg tcaacctcgt
      361 ccaccgcgag cggctcttcc cgctcgaccc ggagggccac gccgagcgcc tgctcggcga
      421 gtcggacggg gtcttccacc ccgcggcgct caacgacaca ttcttcctcg cctgcgccag
      481 cgtcggcccg gacagccgcg ccgtcgccgg ggtctcgctt gccctcaagg cgcggatcgg
      541 aaagctcgct tacggctggg ccctggtccg cctcctgctc cgctggcccc gggcgccgat
      601 ccgcctcgcc tgggacggcg gagcgctcgt ctgcgaggcc ttctacgtcg ccaagggccg
      661 ctatttcgcc ggcccgtgga gcttcgcccc ggaggccgac atcgcccggc ccctgctcca
      721 cgtcgtcgcc ctcgcccggg cccggcgccg cgacttcgcc cgcttcgcct tcgccctctg
      781 gcgcggccgc cgggtcgacg ctttgcccgg gatcaccgcc ttcacctgca ccgcgctgga
      841 ggcggaggcg gacgtgccgc tcccgatcca ggccgacggc gacccggtgg ggactcttcc
      901 ggccagcatc tcgctccacg gggaagcgct gagcctgtgc tgaccgcttg cctccccatc
      961 gaagatgggg agggggacta cccgaagggt ggtggagggg taggaggctg ggcttgggag
     1021 cttcacgcac cgagccgctg agcgctgcgc cagctacccc tcctccccgc tacgcggggt
     1081 ccccctcccc atgcttcgca tggggaggca aatcagcgcg cgcccggctc gtggcggtgc
     1141 tcgcccctga cccagcgcac cgtgcccgtc gacgcccgca tcaccacgct ctcggtcgtg
     1201 atcgcgttct ttcgcctctt caccccctcg agcagggagc cgtcggtgac tccggtcgcg
     1261 gcgaagatgc agtcgccctt ggcgaggtcc tcgagcttgt agactcggtc gaggtcggtc
     1321 acgccccatt tgtccgcgcg cgcccgctcg tcgtcgttgc ggaagatgag ccgcccctgg
     1381 aactggccgc cgacgcagcg caatgccgcc gccgcgagca ccccctccgg cgccccgccc
     1441 gagcccatgt agacgtcgat cgtcgtgtcg ggatcggtga ccgcgatcac gcccgcgacg
     1501 tcgccgtcgg ggatgaggac gatcccgcag cccagcccgc gcaattccgc gatcagcgcc
     1561 tggtggcgct cccggtcgag gacgcagacg atgatctcgg acggatcgac gcccttggcg
     1621 gcggcgaccg agcggatatt ctcggtcgcg ctccggttga ggtcgatcac cccttccgca
     1681 tatcccggcc ccaccgccag cttctccata tagacgtcgg gcgcgttcag gagcccgccc
     1741 tcctcggcga tcgcaagcac ggcgagagcg ttcggagcgc ccttggcggt gatcgtcgtc
     1801 ccctcgagtg gatcgagggc gatgtcgatc ctcgggccgc tgccctgagc cgccccgacc
     1861 ttctcgccga tatagagcat cggcgcctcg tcgcgctcgc cctcgccgat gacgacggtg
     1921 ccgtccatct cgagcgtgtt cagggccgcg cgcatcgcct cgacggcggc atgatcggcg
     1981 gccttctcgt cgccccggcc gatcagcttc gacgccgcga ccgccgccgc ttcggtcacc
     2041 cggaccatct ccaggaccag gacccggtcg agtacgtggc ttgcgctcac catcttgtcg
     2101 ctcatcctcg ctgtaaagaa gcgccgccga taggggagcg ccgccgccgt gtcgagcgcg
     2161 ggcccggcga cccccgtcat tccggcgaag gccggaaccc atgaacaccc aaagtgccga
     2221 attcccgcga tccgtgttca tggatcccgg ccttcgccgg gatggcggaa tccaaggcca
     2281 taattaagcc gttttcctcc aacagcttgg cggacaagca cggcgatgac gaggcgaggc
     2341 tatggcgaag ctctcactgg cggtggcggc cgccgtcctg gcctcggccc cggccgcctc
     2401 tgcgcctccg ccgagcgccg acgaagcgct cgcccgccag cagggctcgc ttcgctccag
     2461 cctcggcctc gactgcgacc gcgattcggg cgagatcgtc gtgtgcggcc gcaccggacc
     2521 cgatcccgac cggctccccc tcccggtccc gcccgagccg ggcgcgcgcc gctccggcga
     2581 gcccgtcgac ccgagcgaca ccctcgccct cagcgccgag acctgcaccg ccgtcggacg
     2641 gaacccgcga tgcagcggcg gcctcccgat cctcggaatc gccgccatga tcgtccagac
     2701 cgtggtgaag gcggctatca aggacgagga atgagggggc ggagcgccgc cttcgcactg
     2761 ctcgcgtccg ccgccgcgcc cgctttcgcc ctcgctcccg aagcccccgt gaccgccgag
     2821 cgggccatgg agatccagaa ggacagcgtc cgcgacgccg tcggcaccct cccctgccgc
     2881 cgcgacgggg ccggcggcga cataatcgtc tgcggccgcc gcggccccga tcccaaccgg
     2941 gtgcccttcc ccgaccaacg gacccccggc gagcccgaga acgtcctccc cggcgagctt
     3001 gcccgcgccc agcccagctt cggcacctgc gagttcggct gccagcagcc ggtcacgatc
     3061 accgacaagg acgtccgcaa atatggcggc cgcatcctgc gccacatcct cggcaaggac
     3121 gattgagaga gcccgtcgtt gcgagaagcg tagcgacgaa acaatccagt cccacgctcg
     3181 cgagaaagga cgcgctggat tgcttcgctt cgttcgcaat gacgg
//
LOCUS       DASWNU010000619         7921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_256916, whole genome shotgun sequence.
ACCESSION   DASWNU010000619 DASWNU010000000
VERSION     DASWNU010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256916"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="gcvPB"
                     /locus_tag="VGB57_12355"
     CDS             <1..1127
                     /gene="gcvPB"
                     /locus_tag="VGB57_12355"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEY0132184.1"
                     /translation="KLTGMHSVAMSPKAGAHGELCGLLAIRSALEARGDAREVILVPE
                     SAHGTNPATAAFVGYRVESIPATDRGRVDFDALKKRLGPDVAAVMITNPNTCGLFEPE
                     MTEISRAVHEAGGLVYCDGANFNAIVGRVRPGDLGIDAMHINLHKTFSTPHGGGGPGS
                     GPVVFSEALTPFAPLPFVEKQGDQFALVEEENAGEHHGSSFGRMVAFHGQMGMFTRAL
                     AYIMSHGADGLRQVSEDAVLNANYILRRLDDLLDAPFGHSGPCMHEALFSDKGFAGGL
                     TTLDLAKGLIDEGFHPMTMYFPLVVHGAMLVEPTETESKAALDQFIGAMRSVAERAKA
                     GDQSLKAAPVHAPRRRLDETLAARKPVLVWKEPPQAEAAE"
     gene            1134..1532
                     /locus_tag="VGB57_12360"
     CDS             1134..1532
                     /locus_tag="VGB57_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0132185.1"
                     /translation="MREPVDIWALYTALNALLMFGLALNVGLRRGAQKQLQPGDMGDA
                     MLTRAIRAHANFAEYAPLVLLLLLGIALLDGPTLWLHALGAAFTIGRVFHVFGMMRGK
                     HPNAVRFIGNLTTGLALLLGAAACLWLALE"
     gene            1569..2201
                     /locus_tag="VGB57_12365"
     CDS             1569..2201
                     /locus_tag="VGB57_12365"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY0132186.1"
                     /translation="MTTSAGRLDNFTDAAFAFAVSLLVIGGAGAPADFNALVLALGDI
                     PAFAFGFAIVVLFWFAHVRWRRLRGEGDWWSLCLTLLLVFLVLIYIQPLRAMAAATAI
                     FFTGQGGGFQGNLTGLFAVYGSGFVLMSLTLAALFAEARRNPNLDRGERAQAKGEVAI
                     WLILAATGIVSILLSLTRFGTFAAFAYSTLPLTIGLFSKRYDWTGRTVEE"
     gene            2198..2788
                     /locus_tag="VGB57_12370"
     CDS             2198..2788
                     /locus_tag="VGB57_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY0132187.1"
                     /translation="MKRSAPATERNRVPIAAVLRETLPERGALLEIASGTGEHAVYFA
                     GCFPTLRWQPTDPDPEALASIRAWREGAGLDNLLEPLPLDAAAETWPVTAADAILCVN
                     MVHISPWAATEGLMRGAGRLLARGGPLILYGPYRRAGVPTAPSNEAFDQSLKARNPQW
                     GLRDLEAVEAEAERHRLRLERVFEMPANNLTMVFRA"
     gene            complement(2885..3130)
                     /locus_tag="VGB57_12375"
     CDS             complement(2885..3130)
                     /locus_tag="VGB57_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132188.1"
                     /translation="MTDRNDRGADRAETARQTDDSDIIDKMEDGPAFGGSAGGNLQRD
                     IGTRDELKEQVGDGDGVTRVRDSDKPEQANLPRFNPK"
     gene            complement(3150..3749)
                     /locus_tag="VGB57_12380"
     CDS             complement(3150..3749)
                     /locus_tag="VGB57_12380"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0132189.1"
                     /translation="MARLLFPLSIGLLGLICIAALLLGGPYTRIDPPILGLFDSPALV
                     PAARLLTHLGDVVTVLAAALIAAGWLLFRGHRRRAILLVVIVAWERLLIEAMKNIFDR
                     ARPDPLGHEVAVHNLAFPSGHSANAMAGWLAIALLAASPRLRRPAIALALAIAFVTGV
                     CRLVLQVHWPSDVAGGWAFGAAWTLLLVRLFGTAPAPRH"
     gene            3867..4028
                     /locus_tag="VGB57_12385"
     CDS             3867..4028
                     /locus_tag="VGB57_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132190.1"
                     /translation="MESNEKYYARRAVEELRALERAVTPEARARRLALAEAFQLKALS
                     GRQPERSTA"
     gene            complement(4557..7829)
                     /locus_tag="VGB57_12390"
     CDS             complement(4557..7829)
                     /locus_tag="VGB57_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132191.1"
                     /translation="MSVKDISQVRQKLVDAGYDADLLAAFSEPQALDGGASEPDSGPE
                     LSRELLTAALGGHYSPGPSTGPGRYAKEHLLDQLLSLSETLLSGSKRVWRLRTDVRRQ
                     ALMRAHRSGELLGAVQAIVPAQDDIAGRILRNVLAGGRIDLDQLPRDEFDALAQVIDW
                     LSGTPFESGLPDVAEVRRVQQRRELIEPFLTLVGRSSDTPQDASRDRFVGREREVERL
                     RAYVGIVPPDKLVDQVKRGLSSLWASVSPGAHSNDPLRIEGTGGSGKSTLVAKFILDH
                     ALIPGVRLPFAYLDFDRATIAPREPLQLLIDIALQLSVWVPEAGESLRLLRHELRDRI
                     EQQAQSREHKDRERQTRSELRGLCRRLKESVESVNAGKAPVLLLLDTFEIVQYDREAI
                     DGVQALIGAMREPEDGPWTNLRVVVAGRGKLSEIRSVQRPLPVGRLSVRATEQLIARR
                     NELDELGLSEAVVAALARPLSESPLDVITITRWLEQNRDKAADFTADLLADPELTEGA
                     ASDVMDARVSALLIDRMIKHIADKRVRSLALPGFVIRAISPAAIAEVMMPAARAKAGS
                     GDTWANRDQGADELFNRLARERWLVYRQGPVLRHRAEVRRAMLRLMRQRDPDAFYSTN
                     DKAIEFFRARAEGDRIARAEAIYHLLLARQPKLEAAEELWRSDVGPVLAGAVDDLQGA
                     AANYLRVKLGRAHGIDELEALPAFVLRSIFASGPALLRRLAPDDCLQLLADRPDAAEQ
                     AVTAGVQAEALYRGGHWAELVSTTASDFHGIVGRGIEQAANGGPVHPTFRAIVLLSTR
                     DSSFDVYWVEWASALRGMLERTTGPFFWEAAALLACLERREPQAPETRVFDVVAQRCR
                     ERSSRGDDWEGNAALRVLAMLEPGGETSILRRVDLKSHLATVNLIELSLFREMLDRAQ
                     AANESTPVAERSVVVIHAIGTGRNILAQSDSKNAGKIILDPQFTSGSGAVAQGLLEAI
                     PDTVANPVRQMLAISHPDWVEPLGLALDRAYGGAVPTRLGWFTSVETLLGSERGGGRH
                     WKTTSGHQILSLADEAGQLLQAVSAYREHMISRVDDPSGFPYLAERLEQWAALLSPHA
                     SA"
BASE COUNT         1279 a         2662 c         2691 g         1289 t
ORIGIN      
        1 tcaagctcac cggcatgcat tcggtggcga tgagcccgaa ggctggtgct catggcgagc
       61 tctgcggcct gctcgcgatc cgctcagccc tggaagcgcg gggtgatgcg cgcgaggtga
      121 tcctcgtgcc ggagagcgcg cacggcacca acccggcgac ggccgccttc gtcggctacc
      181 gggtcgagag catcccggcg accgaccggg ggcgggtcga tttcgacgcc ctgaagaaac
      241 ggctcgggcc ggatgtggcg gcggtgatga tcaccaaccc gaatacctgc gggctgttcg
      301 agccggagat gaccgagatc agccgcgccg ttcacgaagc gggcggcctc gtttattgcg
      361 acggcgccaa tttcaacgcc atcgtcggcc gggtgcggcc cggcgacctc ggcatcgacg
      421 ccatgcacat caacctccac aaaaccttct cgactccgca cggcggcggc ggcccggggt
      481 ccggtccggt ggtcttctcc gaggccctga ccccgttcgc gccgctcccc tttgtcgaga
      541 agcagggcga ccaattcgcc ctggtcgaag aggagaatgc cggcgagcat catggctcca
      601 gcttcggccg gatggtcgcc ttccacggcc agatgggcat gttcacgcgg gcgctggcct
      661 acatcatgag ccacggcgcc gacggcctca ggcaggtgtc ggaggacgcc gtgctcaacg
      721 cgaactacat cctcaggcgg ctcgacgacc tgctcgacgc tccgttcggc cacagcggcc
      781 cgtgcatgca cgaggctttg ttcagcgaca aaggcttcgc cggcggcctc accacgctcg
      841 atctcgccaa gggcctgatc gacgagggct tccacccgat gaccatgtat ttcccgctcg
      901 tcgtccacgg cgccatgctg gtcgagccga ccgagaccga atccaaggcg gcgctcgacc
      961 agttcatcgg cgcgatgcga tcggtggcgg agcgggcgaa agcgggcgac cagagcctga
     1021 aggcggcgcc cgtccacgca ccgaggcggc gtctcgacga gaccctggcg gcgaggaagc
     1081 cggtgctggt ctggaaggag ccgccccagg cggaggccgc ggagtagccg gcggtgcggg
     1141 agcccgtcga catctgggcg ctctatacgg ccctgaacgc gctgctcatg ttcggcctcg
     1201 cgctcaacgt gggattgcgt cggggcgccc agaagcagct ccagcccggc gacatgggcg
     1261 atgcaatgct gacccgggcg atccgtgcgc acgccaattt cgccgaatac gccccgttgg
     1321 tcctgcttct gctgctcggc atcgccttgc tcgacggacc gacgctttgg ctccatgcac
     1381 ttggggcggc cttcacgatc ggccgggtct tccacgtctt cgggatgatg cggggcaagc
     1441 atccaaatgc cgtccgcttc atcggcaatc tgacgaccgg gcttgcgctt ctcctcgggg
     1501 ccgccgcctg cctctggctg gccctggagt agcttgcgcc cattccgggt ccgtcctaac
     1561 cccacgccat gaccacgtcg gcggggcggc tcgataattt taccgacgcg gccttcgcgt
     1621 tcgcggtcag cctgctcgtc atcggcggcg ccggcgctcc ggccgacttc aacgcgctcg
     1681 ttttagccct cggcgacatt cccgccttcg ccttcggttt cgcgatcgtc gtgctgttct
     1741 ggttcgcgca tgtgcgctgg cgccggttgc gcggcgaggg cgactggtgg tccctgtgcc
     1801 tgaccctgct gctcgtcttc ctggtgctga tctacatcca gccgttgcgc gcgatggcgg
     1861 cggcgaccgc gatcttcttc accggacagg gcggcggctt ccagggcaac ctaaccgggc
     1921 tgttcgcggt ctacggcagc ggcttcgtcc tgatgtcgct gacccttgcc gcgctgttcg
     1981 cggaggcgcg gcgcaatccg aatctggatc gcggcgaacg cgcccaggcg aaaggcgagg
     2041 tcgcgatctg gctgatcctg gcggcgacgg gcattgtctc catactgttg tcgctcaccc
     2101 gcttcggcac cttcgccgct ttcgcttatt ccacgcttcc gctcaccatc ggcctctttt
     2161 cgaagcggta cgactggacc ggaaggacag tggaggaatg aaacgctccg cgccggcgac
     2221 cgagcgcaac cgggtgccga tcgcggccgt gctccgcgag acgctgccgg agcggggggc
     2281 tttgctcgag atcgccagcg ggacggggga gcatgccgtc tatttcgccg gatgctttcc
     2341 gacgctgcgc tggcagccga ccgatcccga ccccgaggct ctggcctcga ttcgcgcgtg
     2401 gcgcgagggg gccggcctcg acaatctgct cgagccgttg ccgctggacg cggcggccga
     2461 gacgtggccc gtcaccgccg cggacgcgat cctgtgcgtc aacatggtcc acatcagccc
     2521 ctgggccgcg accgaagggc tgatgcgggg cgcggggcgg ctgctcgcac ggggcgggcc
     2581 gctgatcctt tatgggcctt accggcgagc gggcgtaccg accgcaccga gcaacgaggc
     2641 tttcgatcag tcgctcaagg cccggaaccc gcaatggggg cttcgcgatc tcgaagcggt
     2701 cgaggcggag gcggaacggc accgcctcag gctggagcgg gtcttcgaga tgccggcgaa
     2761 caatttgaca atggtgttcc gagcctaggc cgtcatcccg gcgaaggccg ggatcccggc
     2821 gggtgaaaag cgcaggcgct cttcacccca cggctcccca gccttcgctg gggcgacggg
     2881 caggtcactt cggattgaac ctcggcaggt tcgcctgctc gggcttgtcg ctgtcgcgca
     2941 ccctcgtgac tccgtcgccg tcgccgacct gctccttcag ctcgtcacgg gtgccgatgt
     3001 cgcgctggag gttccccccg gcggagccgc cgaaagcggg gccgtcctcc atcttgtcga
     3061 tgatgtcgct gtcgtccgtc tgccgggcgg tctcggcgcg gtcggcgccg cggtcgttgc
     3121 ggtcggtcat gagcttctcc ttcgcccgat caatgccggg gggcaggagc ggttccgaac
     3181 agacggacga gcagaagcgt ccaggcggcg ccgaacgccc agccgccggc gacgtcgctc
     3241 ggccagtgga cctgcaggac gagcctgcaa accccggtga cgaaggcgat cgccagagcg
     3301 agggctatgg ccggccgccg cagccgcgga ctggcggcga gcagcgcgat ggcgagccag
     3361 cccgccatcg cgttggccga atggccgctc gggaaggcga gattgtggac ggccacctca
     3421 tgtccgagcg gatccggacg ggccctgtcg aagatattct tcatcgcctc gatgagcagg
     3481 cgctcccaag cgacgatgac gacgagcagg atggcgcggc gccgatggcc gcgaaagagc
     3541 agccagccgg cggcgatcaa tgcggctgca agcacggtga cgacatcccc gaggtgagtg
     3601 agcaggcgcg cggcaggtac cagcgccggg gaatcgaaaa ggcccaggat cgggggatcg
     3661 atccgggtat aggggcctcc gagcaacaag gcggcgatgc agatcaggcc gagcagcccg
     3721 atcgacagtg gaaagaggag cctcgccatc gtcgctccct gcccggttgc ggcgccgcct
     3781 gtccagcccg tgcaaaagtc gcgccggatc gattcggact cgtacctgat tcatgttagc
     3841 acatgcgaat cgcgggggag cggtgcatgg aatcgaacga gaaatattat gcccgccggg
     3901 ccgtcgagga gttgcgggcg ctcgagcgcg ccgtgactcc ggaggcgaga gcccggcgac
     3961 tggcgctggc ggaagcgttc cagctcaagg cgctgtcagg ccgccagcct gagaggtcga
     4021 cggcctagcc gctgcgagat cagcacggcg gttcgacgcc gattcaggag agtgacggcg
     4081 cggccggggg cgcggatgcc aggcggcctg tccgccagac ctgcggcgac gagggctagt
     4141 ccgttttttc atcctcattc tcctgctggt cagccaagcc gcgcgggaca ttgcgatcat
     4201 ggcggcggtg ccgggcaaat cgggttttcc tcgggtgagg acggacggtc gatgtggtgg
     4261 acgaggggcc gggtgtgccg gaggctttgc aaccccgcct gctcgaaccc ttttcccgcg
     4321 gcagctcgaa tgccgtggga tgcggcctgg gctccactga cgcgcgggat catgagcgcg
     4381 catggcggcg aggtttcgct ggtgcccggc ccgcgcggag cgacattcag gctaagcttt
     4441 gcgccaccgg acgatgaagg ccgagcctga gaggcccatt tcgcgccaaa cgaagacttc
     4501 gaccgggcgg agaacggagt ccgcccgcaa aggaccagaa acggtcacct gcgcggctac
     4561 gcgctggcgt gcgggctcaa caacgccgcc cattgctcca ggcgctcggc gaggtatgga
     4621 aagccgctcg gatcgtcgac cctcgagatc atatgctccc gataggctga cacggcctgc
     4681 agcaactgtc ccgcctcgtc cgccaatgaa agtatctggt ggccactcgt ggtcttccag
     4741 tgacgcccgc ccccgcgctc gctgccgagg agcgtctcga ccgaggtgaa ccagccaagc
     4801 cgggtcggca ccgcgccgcc gtaagcgcga tcgagagcga ggccaagcgg ctccacccaa
     4861 tcggggtgcg agattgccag catttggcga acggggtttg ctacagtgtc gggaatcgct
     4921 tccagcaaac cttgcgccac cgcgcccgat ccgctggtga attgcggatc gaggatgatc
     4981 ttgcccgcat tcttgctgtc gctttgagcg agaatgttgc gcccggtgcc gatcgcatga
     5041 atgaccacca ccgaccgctc cgcaacgggc gtgctctcgt tcgccgcttg ggcacggtcg
     5101 agcatttcgc ggaacaggct caattcgatc aggttgacgg tcgcaaggtg gctcttcagg
     5161 tcgacccggc gcaggatgga ggtttccccg ccgggttcga gcattgcaag gacgcgcagc
     5221 gcggcgttcc cttcccaatc gtcccctcga gacgatcgct cgcggcagcg ttgggcgacg
     5281 acgtcgaaga ctcgggtctc tggagcttgc ggctcgcgcc gctccaggca ggcaagaagc
     5341 gcggcggctt cccagaagaa cggcccggtc gtccgctcga gcatgccgcg cagcgcagag
     5401 gcccattcaa cccaatagac gtcgaacgac gagtcgcggg tcgagagcag cacgatcgcg
     5461 cggaacgtcg gatgcacggg gccaccgttc gccgcctgct ctatcccccg cccgacgatg
     5521 ccgtggaagt cggacgccgt tgtcgacacc agctcggccc agtgaccgcc gcggtaaagc
     5581 gcttccgcct gaacccccgc cgtcaccgcc tgctccgccg catccggccg atccgccagc
     5641 aattgcaggc aatcgtccgg cgcaagccgc ctgagcagcg ccgggcccga ggcgaaaatc
     5701 gaccgcagga cgaaagccgg gagcgcttcc agctcgtcga tgccgtgcgc tcgcccgagc
     5761 ttcacccgca aataattcgc ggcggcgccc tggaggtcat cgaccgcgcc ggcgagaacc
     5821 ggacccacgt cggaacgcca aagctcctcc gccgcctcca gcttcggctg gcgggcgagc
     5881 aacagatggt agatcgcttc ggccctggcg atccggtcgc cctcggcgcg ggcgcggaag
     5941 aactcgatgg ccttgtcgtt ggtcgagtag aacgcatcgg ggtcacgttg gcgcatcagg
     6001 cgaagcatgg ctcggcgcac ctccgcgcgg tgccggagca ccgggccctg acggtagacg
     6061 agccaacgct cgcgcgcgag gcgattgaat aattcgtcgg cgccctgatc ccggttcgcc
     6121 caggtatcgc ccgatccggc tttcgcccgg gcggcgggca tcatcacctc ggcgatggcg
     6181 gccggtgaaa tggctctgat gacgaagccc ggcagagcga gcgagcggac acgcttgtcg
     6241 gcgatgtgct tgatcatccg gtcgatgagc aacgcgctca cccgagcgtc catgacgtcg
     6301 gatgccgccc cctcggtcag ttcgggatcg gcgaggagat ccgcggtgaa atccgccgcc
     6361 ttgtcgcgat tctgctccag ccaacgagtg atggtgatga cgtccagcgg gctctcgctc
     6421 agcgggcgcg ccagggccgc cacgacggcc tccgacagcc cgagctcgtc cagctcgttg
     6481 cgacgagcaa tcagctgctc cgtcgcgcgc accgagagtc gcccgacggg aagggggcgc
     6541 tggacggagc ggatctcgct cagcttcccc cgcccggcga cgacgacccg caggttcgtc
     6601 caggggccgt cctcgggctc cctcatcgca ccgatgaggg cctgcacacc gtcaatggcc
     6661 tcccggtcgt attgaacgat ctcgaaagtg tccaaaagca gcagcaccgg ggcttttccg
     6721 gcgttaacgc tctcgaccga ttccttcagc cttcggcaaa ggccgcgaag ctccgaccgg
     6781 gtctgacgtt cccggtcctt gtgctcgcgg ctctgtgcct gctgctcgat cctgtcccgc
     6841 agctcgtgcc gcagcagacg gaggctctcg ccggcttccg gcacccagac ggacagctga
     6901 agcgcgatgt cgatgagcag ctggagcggc tcgcgggggg cgatggtggc gcggtcgaaa
     6961 tcgagatagg cgaacggcag acgcacgccc gggatgagcg catgatcgag gatgaacttg
     7021 gcgaccaaag tcgacttgcc tgaaccaccg gtgccttcga tccgaagcgg atcgttggaa
     7081 tgggcgcccg gcgaaacgga agcccacagg ctgctgaggc cacgcttcac ttgatcgacc
     7141 agcttgtccg gaggcacgat tccgacatag gcgcgaagcc gttcgacctc tcgttcccga
     7201 ccgacgaagc gatcgcgcga agcatcttgc ggcgtgtcac tggagcggcc gacaagcgtc
     7261 aggaacggtt cgatcagctc gcgtcgctgc tggacccggc gcacctccgc aacatcgggc
     7321 aggccggact cgaagggcgt gccggacagc cagtcgataa cctgggcgag ggcgtcgaac
     7381 tcgtcccgcg ggagctgatc gaggtcgatt cgacctccgg cgagaacgtt tctcaagatt
     7441 cgcccggcga tatcgtcttg agccggcacg atcgcctgca cggcgccgag gagctcgccg
     7501 gagcggtgcg ccctcatcag cgcctgacgc ctgacatcgg tgcgaagccg ccaaacccgc
     7561 ttgctgccgg acaacagcgt ctcggacaag gagagcaact ggtcgagaag atgctctttc
     7621 gcataccgcc cgggtccggt ggagggaccc ggggaataat ggccgcccag cgctgccgtc
     7681 agcagctccc tggacagctc cgggccgctg tccggctcgg aggcgccgcc gtcgagcgcc
     7741 tgcggctccg aaaaggcggc gagcaggtcg gcgtcgtagc ccgcgtcgac aagcttctgc
     7801 cgcacctggg agatgtcctt gacgctcatc ggcgacgtgc tcgtttcagc attaacgtga
     7861 tcgcgttgcc cgctgcgccc gccagctggg cgaggttatt ctcgttcggg agcgctgtct
     7921 c
//
LOCUS       DASWNU010000620         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_178149, whole genome shotgun sequence.
ACCESSION   DASWNU010000620 DASWNU010000000
VERSION     DASWNU010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178149"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /gene="nadB"
                     /locus_tag="VGB57_12395"
     CDS             <1..1539
                     /gene="nadB"
                     /locus_tag="VGB57_12395"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY0132192.1"
                     /translation="SLDPSLRVAVLAKGSVSSGSTALAQGGIAAVLEPGDTFDSHIKD
                     TIVAGAGLNRRETVEFVVESAPAAIERLAALGVPFNKGEEAGWHLTREGGHSHRRIVH
                     VADATGWAVQQALEAAAADHPNIQLIPNRVAVDLVTGRHTVSGGEDGRVRGVYALNRE
                     TGRVELFSGRATILASGGASRAWLYSTNPRGSTGDGIAMAWRAGCRVANMEFNQFHPT
                     CLYHHDVKNFLITEAMRGEGGRLKLPGQGGNGGRRFMKRFDPREELAPRDIVARAIDH
                     ELKRLGLDFVHLDISHRDPDFVRAHFPNIAARLAEPDIGIDITREPIPVVPAAHYTCG
                     GVMTDLDGRTDVEGLYAAGEVTMSGLHGANRLASNSVLECLVFGVAAAEHINASWDRL
                     PPPPVIRPWDESRVTDSDEEVVVQHNWREIRRFMWDYVGIVRTTKRLERALNRINLLK
                     QETHDYYRHFRVTADLVELRNLVDVAELIVRSALTRQESRGLHYTLDYPELLPDAVDT
                     VLVP"
     gene            complement(1705..>2358)
                     /locus_tag="VGB57_12400"
     CDS             complement(1705..>2358)
                     /locus_tag="VGB57_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0132193.1"
                     /translation="VAAVPGYRYHLVQVAGFANTPARGNARGDVVVPLADEVARYISS
                     RGLRRPAIVGHSMGGTVAMLVASRHPSLPGKVMVVDMLPQPAGILGGDAAGMRGLADS
                     LRDLSASPGGRRLVESAIRLFGNPEGGADPDVTARATHELALMDLTPQLPRIAAPLTI
                     VYASLDASRRAADDGRYRTAYAGKRGVELVRVDNSGHMIMYDQPARFRAELKSFLAR"
BASE COUNT          374 a          855 c          780 g          349 t
ORIGIN      
        1 agcctcgatc cgtcgctgcg cgtcgccgtg ctcgccaagg gcagcgtttc ctccggatcg
       61 accgcgctcg cccaaggggg aatcgccgcg gtgctggagc cgggggatac gttcgatagc
      121 catatcaagg acacgatcgt cgccggcgcc gggctcaacc ggcgcgagac ggtcgaattc
      181 gtcgtcgaga gcgcccccgc cgcgatcgag cggctcgccg cgctcggcgt tcccttcaac
      241 aagggcgagg aagcgggctg gcacctgact cgcgagggcg gccacagcca tcgccggatc
      301 gtccatgtcg ccgacgcgac cggctgggcg gtccagcagg cgctcgaagc ggcggccgcc
      361 gaccatccca atatccagct gatcccgaat cgggtcgcgg tcgacctcgt caccggccgc
      421 cacaccgtct ccggcggtga ggacggaagg gtacgcggcg tctacgcgct caaccgggag
      481 accggccggg tcgagctgtt cagcggccgg gccaccatcc tcgccagcgg cggcgccagc
      541 cgcgcctggc tctattcgac caacccccgc ggctcgaccg gcgacggaat cgccatggcc
      601 tggcgggcgg ggtgccgggt cgcgaacatg gaattcaacc agttccaccc gacctgcctc
      661 taccaccatg acgtcaagaa cttcctgatc accgaggcga tgcgcggcga gggcggcagg
      721 ctcaagcttc ccggccaagg cgggaatggg ggccgccgct tcatgaagcg cttcgatccc
      781 cgcgaggagc tcgccccccg cgacatcgtc gcccgcgcca tcgatcacga gctgaagcgg
      841 ctcggcctcg acttcgtcca cctcgacatc agccaccgcg atcccgattt cgtccgcgcc
      901 cacttcccca acatcgccgc ccgcctggcg gagccggaca tcggcatcga tatcacccgc
      961 gagccgatcc ccgtcgtccc ggccgcccat tatacgtgcg gcggagtgat gaccgacctc
     1021 gacggccgca ccgacgtcga gggcctctac gccgccggcg aggtcaccat gtcgggcctc
     1081 cacggcgcca accgcctcgc ttcgaattcg gtgctcgaat gcctcgtctt cggagtggcc
     1141 gccgccgagc acatcaacgc gagctgggac cgcctcccgc cgccgcccgt catccggcca
     1201 tgggacgaga gccgagtgac cgattcagac gaggaagtgg tcgtccagca caattggcgc
     1261 gagatccgcc gcttcatgtg ggattatgtc gggatcgtcc ggaccaccaa aaggctcgag
     1321 cgcgccctga accggatcaa cctcctcaag caggagaccc acgattatta ccgccacttc
     1381 cgggtcacgg ccgacctcgt cgagcttcgc aacctggtcg acgtcgcgga gctgatcgtc
     1441 cgctcggccc tcacccgcca ggagagccgc ggcctccact atacgctcga ctatccggag
     1501 ttgctgccgg atgcggtgga tacggtcttg gtgccctgac ttgcctcccc gtcgaagatg
     1561 gggaggggga ccaccgaagg tggtggaggg gtagaaggcg cggaagtggc gggttcgaga
     1621 gggaacctcc ttgtcggcgc cagttacccc tccaccaggc ttcgcctggt ccccctcccc
     1681 accgcttcgc ggcagggagg caagtcatcg tgccagaaag ctcttcagct ccgccctgaa
     1741 ccgggccggc tggtcgtaca tgatcatgtg gccgctgttg tcgacccgga ccagctccac
     1801 gccccgcttc cccgcgtaag ccgttcgata gcggccgtcg tcggcggctc ggcggctggc
     1861 atctaggctt gcatagacga ttgtgagcgg cgccgcgatc cgcggcagct gcggagtgag
     1921 gtccatcagt gccagctcgt gagtggcccg ggcggtcacg tccggatcgg cgccgccctc
     1981 cggattgccg aacagcctta tcgccgattc gaccagccgc cgcccgccgg gggaggcgct
     2041 gaggtcgcgc aggctgtcgg ccagtccgcg catcccggcg gcgtcgccgc cgaggatgcc
     2101 cgcgggctgg ggcagcatgt cgaccaccat caccttgccg ggaaggctgg ggtggcgcga
     2161 ggcgaccagc atcgcgaccg tgccgcccat cgaatggccg acgatggcgg gccggcgaag
     2221 cccgcgcgag cttatgtagc gcgcgacctc gtcggcgagc gggacgacga catcgccccg
     2281 cgcattgccg cgcgcgggcg tgttcgcgaa gcccgccacc tggacgagat ggtagcgata
     2341 gcccggcacc gccgcgac
//
LOCUS       DASWNU010000621         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_205729, whole genome shotgun sequence.
ACCESSION   DASWNU010000621 DASWNU010000000
VERSION     DASWNU010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205729"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VGB57_12405"
     CDS             <1..335
                     /locus_tag="VGB57_12405"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY0132194.1"
                     /translation="IPGTFGTPPSGYFIYDSSGHCSVQIMTVPPVKIVTPDQPTAPEA
                     LAIFNGYIAYYGTYTVKDGNIHQQVEGAWDPSQVGTDQVRPFELKDADTLIIGNQTTY
                     RRTLKRVR"
     gene            540..1919
                     /locus_tag="VGB57_12410"
     CDS             540..1919
                     /locus_tag="VGB57_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEY0132195.1"
                     /translation="MRRLFLLLSASLAAPASAQSGQGDLSVTIYNQNLALVQDTRQLN
                     LPAGRSRQEFPNVSGQISPETVTLSGQGVGIVEQNFDFDLLSPSKLMEKAVGEEITLL
                     RTNPATGAETRERARVLAVNGGVVLQIGNRIEVLRDDGLPVRAIFDRVPENLRARPTL
                     SVTLESARAGARPVTLSYLTPGMSWKADYVTLFDEAAGKIDVQGWVTLTNTTGTTFDN
                     ADTLLVAGNIGGRGGYDSDGGGYRPPPPPGNRPGTETPGRESLGDFLLYPLKERTTIA
                     NQQTKQVSFLDVSGAAGRRAYVFRNSWLGTLDQAASADTVLQFSSSRNQGLGDALPAG
                     TVRVYMKDSRGNAQFIGENQIGHTPMGSDVAITTGQAFDVKVQPVVEKRERLSSGRWR
                     TTMRYTLTNARPQPATVDVIQAGLWGDVRIADESLKSIRRSADEAMWRVTVPANGEAT
                     VTATFDTRY"
     gene            1933..>2256
                     /locus_tag="VGB57_12415"
     CDS             1933..>2256
                     /locus_tag="VGB57_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132196.1"
                     /translation="MRRAMLGSLLLAAAAAAGAAEPVARSPGPEAVAVTVYRAPGRGA
                     DQGMDLRWLNGFALVTETRRISIPAGESDVRFEGVAGGILPESAIVSGFPDGVREKNQ
                     DAWLLS"
BASE COUNT          389 a          783 c          732 g          352 t
ORIGIN      
        1 ggattcccgg cacgttcggg actccgccga gcggctattt catctacgat tcttccggtc
       61 attgctcggt gcagatcatg accgtcccgc cggtcaagat cgtgacgccc gaccagccta
      121 cggcgcccga ggcgctcgcc atattcaatg gctatatcgc ttattacggc acctacacgg
      181 tcaaggacgg taacatccac caacaggtcg aaggcgcctg ggatccctcc caggtcggca
      241 ccgatcaggt gcgccccttc gagctcaagg acgccgatac cctgatcatc ggcaaccaaa
      301 ccacctaccg ccgcaccttg aagcgggttc gctagagtct atccggataa ggtggaatcg
      361 tttcccggcg aaggccggga tccagtcttt gcagccgtgc cgctgggccc cggccttcgc
      421 cggggaacag gtgtttcaat atggtccgga ccgactctgg cggcgctcca attctagccg
      481 ttgacagttc aggttacatt gtaacatgct cccgcccgtc gatgccaggg agcctgtcca
      541 tgcgccgcct gttcctcctt ctctccgcct ccctcgccgc gccggcctcc gcccaaagcg
      601 gtcagggcga cctctcggtc accatctaca accagaatct ggcgctggtg caggacacgc
      661 ggcagctgaa cctccccgcc ggccgctctc gccaggaatt tcccaacgtg tcgggccaga
      721 tcagcccgga gacggtgacg ttgagcgggc agggcgtcgg catcgtcgag cagaatttcg
      781 acttcgacct gctttcccca tccaagctga tggagaaggc ggtgggggag gagatcaccc
      841 tgcttcgaac caaccccgcg accggcgccg agacccggga gcgggccagg gtgctcgcgg
      901 tcaacggcgg agtcgtcctt cagatcggca accggatcga agtgctccgc gacgacggct
      961 tgcccgtccg cgccatcttc gatcgggttc cggaaaatct gcgcgcccgg ccgacgctct
     1021 ccgtcaccct ggagagcgcc cgcgccggcg cccggccggt caccctcagc tacctgacgc
     1081 caggcatgag ctggaaggcg gactacgtca ctttgttcga cgaggcggca ggcaagatcg
     1141 acgtccaggg ttgggtgacc ctgaccaata cgaccgggac caccttcgac aatgccgaca
     1201 ccctgctcgt cgccggtaat ataggtgggc gcggcggcta cgattccgat ggcggaggct
     1261 atcgcccacc gccacccccg ggaaaccggc caggcaccga aacgccgggc cgcgaaagcc
     1321 tgggggactt cctgctctac ccgctcaagg aacggaccac gatcgcgaac cagcagacca
     1381 agcaggtgag cttcctcgac gtttccggcg ccgccggccg gcgcgcctac gtcttccgaa
     1441 attcctggct gggcacgctg gatcaggcgg cgagcgccga caccgtcctg caattctcga
     1501 gctcgcgcaa ccagggcctg ggcgacgccc tgccggcggg gacggtcagg gtctacatga
     1561 aggattcgcg cggcaacgcg cagttcatcg gcgagaacca gatcgggcac acaccgatgg
     1621 gctcggacgt ggcgatcacc acgggccagg ccttcgacgt caaggtccag ccggtcgtcg
     1681 agaagcgcga aaggctgtcg agcggccgct ggcgcacgac catgcgctac acgctcacca
     1741 acgcccggcc gcagcccgcg acggtcgacg tgattcaggc ggggctgtgg ggcgacgtcc
     1801 ggatcgccga tgagagcctg aagagcattc gccgctcggc cgacgaggcg atgtggcggg
     1861 tgacggtccc ggccaatggc gaggcgaccg tgaccgccac cttcgacacg cgctactgac
     1921 gagcgcgggg ccgtgcgccg tgccatgctt gggagcctgc tgctcgccgc ggcggcggcc
     1981 gcgggcgccg cggagccggt ggcgcgttcg ccggggccgg aagcggtcgc ggtcaccgtc
     2041 taccgcgcgc ccggccgcgg cgcggaccag ggcatggatc tgcgctggct gaacggcttc
     2101 gccctggtca ccgaaacccg gcgaatctcg atcccggccg gggaatcgga cgtccgcttc
     2161 gaaggcgtcg ccggcgggat cctccccgaa agcgcgatcg tcagcggctt tccggacgga
     2221 gtgagggaga agaaccagga cgcctggctg ctctcc
//
LOCUS       DASWNU010000622         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_257059, whole genome shotgun sequence.
ACCESSION   DASWNU010000622 DASWNU010000000
VERSION     DASWNU010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257059"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGB57_12420"
     CDS             complement(<1..684)
                     /locus_tag="VGB57_12420"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0132197.1"
                     /translation="MKLLAGNSNLPLVKSIAAYLELPVTEASVRRFADEEVFVEIHEN
                     VRGEDVFVVQSTSYPANDNLMELLICIDALRRASAKRITAVLPYFGYARQDRKPGPRT
                     PISAKLVANLITVAGANRVLSVDLHAGQIQGFFDIPTDNLYGAPVMSEDIKARFNGRA
                     ITVVSPDVGGVVRARALAKRLANAPLAIVDKRREKPGESEVMNIIGDVGGRFCVLVDD
                     IVDSAGTLCN"
     gene            complement(684..1553)
                     /locus_tag="VGB57_12425"
     CDS             complement(684..1553)
                     /locus_tag="VGB57_12425"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0132198.1"
                     /translation="MGRLRRIAIELFVLAAVGTLLGLLGPFGSDRLAAPWRLFFWVGF
                     VLAGYLIFRPVSAVARWLTEETRVPPWLAVLLVAVVASLPLATIIAFALAGLTGADFW
                     GGSRFPLLYGQVALVGVCIHLLMRLVFGSAPTRSAAAPDNGTAAAAVASQSDQVGNPF
                     LQRLPPALGRDLLCLQMQDHYVQAQTALGTTLILMRFRDAVAELGDSGVQVHRSWWAA
                     FDAMEALDRDGRNGRLRLRGGGTIPVSRACLPTVRDALRHGPPGGFDRQAGSSKSSGA
                     CGDSAGGTIAEIL"
     gene            1713..2216
                     /locus_tag="VGB57_12430"
     CDS             1713..2216
                     /locus_tag="VGB57_12430"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEY0132199.1"
                     /translation="MAVLDRVDGGGWARPAAALGIATAATLLFWGLSLLIERPDGWRP
                     PAGADVALYIHLFTVIPAVPLGAVVLWRTKGGRVHRLLGRIWGALMMVTAVSSFWLQS
                     LSGGLSFIHLFAVLTLISIPLAVWNARRGNIRAHRNAMRGVYAGLIAAGLLAVLPGRT
                     LGTLLFG"
     gene            2256..>3300
                     /gene="gltX"
                     /locus_tag="VGB57_12435"
     CDS             2256..>3300
                     /gene="gltX"
                     /locus_tag="VGB57_12435"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0132200.1"
                     /translation="MPVVTRFAPAPTGRLHVGNIRTAIHNWLWARKNDGRFILRLDDT
                     DKERSSEANAEGIREDLAWLGLAWDEEHRQSLRFDRYEAAFERLREAGRVYPAYETAQ
                     ELDLKRKVQLSRGKPPVYDRAALQLSGAERARLESEGRRPHWRFRLDHDSPIEWDDLI
                     RGRVHFEAALLSDPVVRREDGSWLYMLPSSVDDVDMGVTHVLRGEDHVANTALQLQMF
                     EAMGAGPPAFAHEALLVGSEGKLSKRLGSLGADSLREQGIEPIALVAKLARLGTSLPV
                     EPATEPGTLIETFDFATFGRAPARFDLDELVQLNARIVHLLPFESVAARLPEGMGPDD
                     WAAIRPNLKSVAEA"
BASE COUNT          530 a         1096 c         1178 g          496 t
ORIGIN      
        1 gttgcacagg gttccggccg aatcgacgat gtcgtcgacg aggacgcaga agcggccgcc
       61 gacgtcgccg atgatgttca tcacctccga ttcgccgggc ttctcgcggc gcttgtcgac
      121 gatggcgagc ggggcgttgg cgagcctctt ggccagcgcc cgggcgcgca ccactccgcc
      181 gacgtcgggc gagacgacgg tgatggcgcg gccgttgaag cgcgccttga tgtcctcgga
      241 catgaccgga gcgccataga ggttgtcggt cgggatatcg aagaagccct ggatctggcc
      301 ggcatgaagg tcgaccgaaa ggacccggtt ggcgccggcg accgtgatca ggttggcgac
      361 cagcttggcg gagatgggcg ttcgcggccc gggcttacgg tcctggcggg catagccgaa
      421 ataggggagg acggcggtga tccgcttcgc cgaggcgcgg cgaagcgcgt cgatgcagat
      481 caaaagctcc atcagattgt cgttggcggg gtaggaggtc gactggacca cgaacacgtc
      541 ctcgcctcgg acgttctcgt ggatctcgac gaacacctcc tcgtcggcga atcggcggac
      601 gctcgcttcg gtgacaggaa gctcgagata ggcggcgatg ctcttcacca gcggcaggtt
      661 cgaattgccg gccaagagct tcattacagg atctccgcga tggtgccgcc ggccgagtcg
      721 ccgcaggcgc cggaactctt agaggacccg gcctgcctgt caaacccgcc gggaggccca
      781 tggcgaaggg cgtcgcggac cgtcgggagg caggcgcggg agaccggaat cgtgccgccg
      841 ccgcggagcc gaaggcgtcc gttccgcccg tcgcggtcga gggcttccat ggcgtcgaag
      901 gcggcccacc agctgcggtg gacctggacg cccgagtcgc cgagctccgc gaccgcgtcg
      961 cgaaagcgca tcaaaatgag agtcgtgccg agcgccgttt gcgcctgcac ataatggtcc
     1021 tgcatctgga gacagaggag atcgcgcccc aaagcgggcg gcaatcgttg caggaacgga
     1081 ttgccgacct ggtcggattg cgatgcgact gcggcggcgg ccgttccgtt atccggcgcg
     1141 gcggcggagc gggtgggcgc tgagccgaag acgagccgca tcagcaggtg gatgcagact
     1201 ccgaccagcg ccacctggcc gtagagcagg gggaagcggc ttccgcccca gaagtccgcg
     1261 ccggtcagtc cggccagcgc gaaggcgatg atcgtcgcca gcggcagcga ggcgaccacg
     1321 gccaccagca ggaccgcgag ccagggcgga acccgggtct cctcggtgag ccagcgcgcg
     1381 accgcggaga cggggcggaa gatgagatag ccggcaagaa cgaacccgac ccagaagaac
     1441 agccgccaag gcgcggcgag cctgtcgctg ccgaacggcc cgagcaggcc gagcaaggtc
     1501 cccaccgccg ccagcacgaa cagctcgatc gcgattcgcc gcagccgccc catccttcct
     1561 cctgcccgtt cacgcttcgc gaacgggacg attagcgcga aaccgccggc ttgcgaaaga
     1621 gaagcgcccg cccgcgactt cggccttgcc ggcgaacggc cgccggccat cctcgcctcg
     1681 gacgcggcaa tccgccgccg ggaggatgga agatggcagt gctggatcga gtggatggag
     1741 gcggctgggc gcgacccgcg gcggcgttgg ggatcgcgac cgcggccacc ctgctattct
     1801 ggggcctgtc gctgctgatc gagcgcccgg acggctggcg gccgcccgcc ggagccgatg
     1861 tcgcgctcta catccacctc ttcaccgtga tccccgcggt tccactgggc gccgtcgtgc
     1921 tgtggcggac caagggcggc cgggtccaca ggctgctggg caggatctgg ggagctttga
     1981 tgatggtgac cgccgtctcc agcttctggc tgcagagcct gagcggcgga ttgagcttca
     2041 tccacctgtt cgcggtgctc accctgatct cgatcccgct cgccgtgtgg aatgcgcggc
     2101 gcggcaacat acgcgcgcac aggaacgcga tgcgcggagt ctatgcgggc ctcatcgcgg
     2161 cggggctgct ggccgtgctg cccggccgca cccttggaac cttgctgttc ggttgaagcg
     2221 ttgccgccac ccgatgccgc gcctaaacgg ccggaatgcc cgtcgtcacc cgcttcgccc
     2281 cagcgcccac cggacgcctg cacgtcggaa acatccggac cgcgatccat aactggctgt
     2341 gggcgaggaa gaatgacggc cgcttcatcc tgcgtctcga cgacaccgac aaggagcgtt
     2401 cgagcgaggc gaatgcggag gggatccgcg aggatctggc ctggctcggt ctcgcctggg
     2461 acgaggagca tcgccagtcg ctccgcttcg accgctacga ggccgcgttc gaaaggcttc
     2521 gggaggccgg ccgggtgtat ccggcgtacg agacggcgca ggagctcgat ctcaagcgca
     2581 aggtccagct ctcccgcggc aagcccccgg tctatgaccg cgccgcgctt cagctttccg
     2641 gggcggagcg ggcgcggctc gagtcggagg ggcgacggcc gcactggcgc ttccggctcg
     2701 atcacgattc gccgatcgag tgggacgatc tcatccgggg ccgggtgcat ttcgaggccg
     2761 ccttgctctc cgacccggtg gttcgccgcg aggacggcag ctggctctac atgcttccga
     2821 gcagcgtcga cgatgtcgac atgggcgtga cccacgtgct tcgcggcgag gaccatgtcg
     2881 ccaacaccgc gctccagctg cagatgttcg aggcgatggg agcgggtccc ccggccttcg
     2941 cccacgaggc gctgctggtc ggatccgagg gcaagctgtc gaagcggctc ggctcgctcg
     3001 gcgcagattc gctgcgcgag caggggatcg agccgatcgc cctggtcgcc aagctcgccc
     3061 ggctcggcac cagccttccg gtcgagccgg cgacggagcc cgggacgctg atcgagacgt
     3121 tcgatttcgc cacgttcggc cgggcgccgg cgcgtttcga tctcgacgag ctcgtccagc
     3181 tcaacgctcg gatcgtccac cttctgccct tcgagtcggt cgccgcccgg cttccggaag
     3241 gcatggggcc ggacgactgg gccgcgatcc ggcccaatct gaagtcggtg gcggaggcgg
//
LOCUS       DASWNU010000623         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_308805, whole genome shotgun sequence.
ACCESSION   DASWNU010000623 DASWNU010000000
VERSION     DASWNU010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308805"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGB57_12440"
     CDS             complement(<1..287)
                     /locus_tag="VGB57_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0132201.1"
                     /translation="MNDMTKLTAKDFATDQEVRWCPGCGDYAVLKAVQRTMPELGVAP
                     ENVVFVSGIGCSSRFPYYMETYGFHTIHGRAPTFATGIKLANPELDVWIITG"
     gene            complement(553..>2011)
                     /locus_tag="VGB57_12445"
     CDS             complement(553..>2011)
                     /locus_tag="VGB57_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY0132202.1"
                     /translation="LARWQVISFNISQLTLDAVKPFGLGNKEALRCKNMWTLGLARWM
                     FDREREPIVDWLKSKFAKAPELAGANIAALNAGHAYGETVEIGGQIHQQHLEPAPVAP
                     GLYRTVTGAEAISIGLVAGAQLANLPMFFGGYPITPASAILHHLSRLKEYGVTTFQAE
                     DEIAAIGAAIGASFAGSLGVTSSSGPGIALKAEAMGLAIMTELPLVIVNSQRGGPSTG
                     LPTKTEQSDLYQAVYGRNGDAPMPVIAARSPADAFDCAIEAVRIATQYMTPVMLLTDG
                     YIANAAEPWRVPDMATYAPFPVRFHDEAPAEGEKVNPYARDGKLARVWIRPGTYGLRH
                     RIGGIEKNVGTGHIDYAPANHQAMTDIRANKVLGVADSIPEQEVCLGGPGGALAVVGW
                     GSTFGPIHQAVRRARARGVDVNHIHIRHIWPLPRNLGDLLHGFGRVIVPEMNKGQLKT
                     LLRDQYLIDARPVTKVSGQPFKIAEIEAAIEEALG"
BASE COUNT          326 a          647 c          704 g          334 t
ORIGIN      
        1 ccggtgatga tccacacgtc gagctccgga ttggcgagtt tgatgcccgt cgcgaaggtc
       61 ggcgcccggc cgtggatcgt gtggaagccg tatgtctcca tataataagg gaagcggctc
      121 gagcagccga tcccggagac gaacacgaca ttctccggcg cgacgccgag ctccggcatg
      181 gtccgctgca ccgccttaag caccgcataa tcgccgcacc ccgggcacca gcgcacctcc
      241 tgatcggtgg cgaagtcctt ggcggtgagc ttggtcatgt cgttcattgg gtgggcttac
      301 ctgtaaagaa ggtaccagat caaagcgcag acgatgatca ggagcaggac gaggcggatg
      361 aggccggggg tggtcatggg gcaccaagtg atgggttaag aaggagcggt ccgtccccaa
      421 ccgtcatccc gtcgaaggcc gggatcccag cgggtgaaag gcgggggcgg aagtggagcg
      481 ttccacttgc agcggcgccc ttcacaccct gaggtcccgg cctgccccgg gacgacctga
      541 cctggaccgc cctcatccga gcgcctcctc gatggcggct tcgatctcgg cgatcttgaa
      601 gggttgcccg gagaccttgg tgacagggcg cgcgtcgatc agatattggt cgcggagcag
      661 agtcttcagc tgccccttgt tcatctccgg aacgatgacg cgcccgaatc cgtggagcag
      721 gtcgccgaga ttcctcggca gcggccagat gtggcggatg tggatatggt tgacgtcgac
      781 gccgcgcgcc cgcgcgcggc ggacggcctg gtggatcggc ccgaaggtcg agccccagcc
      841 gaccacggcg agcgccccgc ccggcccgcc gaggcacacc tcctgctccg ggatcgaatc
      901 ggcgacgccc agcaccttgt tggcgcggat gtcggtcatc gcctggtggt tggcgggcgc
      961 ataatcgata tggccggtgc cgacattctt ctcgatgccg ccgatccggt gccggaggcc
     1021 gtaggtgccg ggcctgatcc agactcgggc cagctttccg tcgcgcgcgt agggattgac
     1081 cttctcgccc tcggccggcg cctcgtcatg gaagcgcacc gggaacggcg cataggtcgc
     1141 catgtccggg acgcgccagg gctcggccgc attggcgata tagccgtcgg tcagcagcat
     1201 caccggagtc atgtactggg tggcgatccg caccgcctcg atcgcgcagt cgaacgcgtc
     1261 ggcgggcgag cgcgcagcga tcaccggcat cggcgcgtcg ccgttgcggc cgtagaccgc
     1321 ctggtagagg tcggactgct cggtcttggt cggaaggccc gtcgaagggc ccccgcgctg
     1381 cgagttgacg atgacgagcg gaagctcggt catgatcgcc aggcccattg cctccgcctt
     1441 gagcgcgatc cccgggccgg acgaggaggt cacgccgagg ctgccggcga agctggcgcc
     1501 gatcgccgcg ccgattgcgg cgatctcgtc ctccgcctgg aaagtggtga ctccatattc
     1561 cttgaggcgc gagagatggt ggaggatcgc gctggccggc gtgatcggat agcccccgaa
     1621 gaacatcggc aggttcgcga gctgcgcacc ggcgaccagc ccgatcgaga tcgcctcggc
     1681 cccggtgacg gtgcggtaga ggcccggcgc gaccggcgcg ggctccagat gctgctggtg
     1741 gatctggccg ccgatctcga ccgtctcgcc ataggcatgg cccgcgttga gcgctgcgat
     1801 gttcgccccg gcgagctccg gcgccttggc gaacttggac ttcagccagt cgacgatcgg
     1861 ctcgcgctcg cggtcgaaca tccagcgcgc gagaccgagc gtccacatgt tcttgcagcg
     1921 caaggcctcc ttgttgccga ggccgaaggg cttcaccgcg tcgagcgtaa gctgcgagat
     1981 gttgaaggag atgacctgcc acctggcgag g
//
LOCUS       DASWNU010000624         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_257258, whole genome shotgun sequence.
ACCESSION   DASWNU010000624 DASWNU010000000
VERSION     DASWNU010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257258"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2023
                     /locus_tag="VGB57_12450"
     CDS             <1..>2023
                     /locus_tag="VGB57_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0132203.1"
                     /translation="ARYAALVGRKLRLPITGRLIPIIRDEHADPELGSGAVKITPGHD
                     FNDFEVGRRAGFKATEMLNMLSGDARVVQTRDGLIPEELIGLDRFEARKRVVERLEAD
                     GLLERVEDRVIQTPYGDRSGVVIEPWLTDQWYVDAKTLAGPAIEAVRSADIRVVPRTW
                     EKTWFNWLENIQPWCVSRQLWWGHQIPAWYDDQGNVFVAETEEEAQREAGNRPLRRDP
                     DVLDTWFSSALWPFATLGWPNDAEPLSPAGRGQGEGAPTLEDGLGGRTPSPQPSPPGG
                     EGVKWKPKPGTELARHYPNDVLISGFDIIFFWDARMAMQGFHFLGEKPWKTLYLHGLV
                     RDSKGQKMSKSKGNTVDPLGLIDRYGADALRFTLAAMESQGRDIKLDEKRVEGYRNFA
                     TKLWNAARFCQANGIGASVGLEPPEAELPVNRWIIGETVKTVQSLDLSLAELRFDESA
                     NGLYHFVWATFCDWYLELIKGQIDEETKAVAAWVLDQILVMLHPFMPFITEELWNANA
                     RPYPLIVAKWPMPDARALDPEAGPEIDWLIRLVSGIRAARAELNVPPGAKLPLHVRDC
                     GDGTRGRLERHDPAIRRLARVEAVYFGEASGGAAQVVVDEATFILPLEGVIDIEAERA
                     RLSKALQAAEKERDSLAARLANPSFVERAKPEAVEKAKADHAEKASDAE"
BASE COUNT          360 a          664 c          698 g          301 t
ORIGIN      
        1 atgcgcgcta tgccgccctc gtcggaagga agctgaggct tccgatcacg ggcaggctga
       61 taccgatcat ccgcgacgag catgccgatc ccgagctcgg ctcgggggcg gtcaagatca
      121 cgcccggcca cgatttcaac gatttcgaag tcggccggag ggcgggattc aaggcgaccg
      181 agatgctcaa catgctgagc ggcgatgcca gggtagtgca gacccgggat gggctcattc
      241 cggaggagct gatcggactc gaccgcttcg aggcgaggaa gcgggtcgtc gagaggctcg
      301 aagccgacgg cctcctcgag cgagtcgagg atcgagtcat ccagacgccc tatggcgacc
      361 gttcgggggt ggtgatcgag ccctggctga ccgaccaatg gtacgtcgac gccaagaccc
      421 tcgccgggcc ggcgatcgag gcggtgcgct cggccgacat ccgggtggtg cccaggacct
      481 gggagaagac ctggttcaac tggctcgaga acatccagcc ctggtgcgtc tcgcgccagc
      541 tctggtgggg gcaccagatc cccgcctggt atgacgacca ggggaacgtc ttcgtcgccg
      601 agacggagga ggaggcgcag cgcgaggcgg gcaatcgccc gcttcggcgc gatcccgacg
      661 tgctcgacac ctggttctcc tccgccctgt ggccgttcgc cactctcggc tggcccaacg
      721 atgcggaacc cctctccccg gcggggagag ggcagggtga gggggctccc acgcttgagg
      781 acggactcgg cggccgaacc ccctcacccc aaccctctcc cccaggggga gagggggtga
      841 agtggaagcc caagcccggc accgagcttg cccgccatta tccgaacgac gtgctcatct
      901 ccggcttcga catcatcttc ttctgggacg cccggatggc gatgcagggc ttccacttcc
      961 tgggcgagaa gccctggaag acgctctacc tccacggcct cgtccgcgac tccaagggcc
     1021 agaagatgtc caagtcgaag ggcaacaccg tcgacccgct cggcctcatc gaccgctacg
     1081 gcgccgacgc gcttcgcttc actttggcgg cgatggagag ccagggccgc gacatcaagc
     1141 tcgatgagaa gcgagtcgag ggctacagga acttcgccac caagctgtgg aacgccgccc
     1201 gcttctgcca ggccaacggc atcggcgcga gcgtcgggct ggagccgccg gaggccgagc
     1261 tcccggtcaa ccgctggata atcggggaga ccgtgaagac ggtgcagtcg ctcgacctca
     1321 gcctcgccga gcttcgcttc gacgagagcg cgaacggcct ctaccatttc gtctgggcga
     1381 ccttctgcga ctggtatctg gagctgatca agggccagat cgacgaggag acgaaggccg
     1441 tcgcggcctg ggtgctcgac cagatactgg tcatgctcca cccgttcatg cccttcatca
     1501 ccgaagaatt gtggaacgcc aacgcgcggc cctacccgct gatcgtcgcc aaatggccga
     1561 tgcccgacgc tcgggcgctc gaccctgagg cggggcccga gatcgactgg ctgatcaggc
     1621 tggtgagcgg catccgggcc gcccgggcgg agctgaacgt gccgcccggc gcgaaattgc
     1681 cgcttcacgt cagggattgc ggggatggaa cgcgcggtcg gctggagcgc cacgatccgg
     1741 cgatccggcg gctcgcccgg gtcgaagcgg tctacttcgg cgaggcctcg ggcggggcgg
     1801 ctcaggtggt cgtcgacgaa gcgaccttca tcctgccgct cgaaggcgtg atcgacatcg
     1861 aggcggagcg ggcgcggctc tccaaggccc ttcaggcggc ggagaaggaa cgcgattcgc
     1921 tcgccgcccg cctcgccaat cccagcttcg tcgagcgggc gaagccggag gcggtggaga
     1981 aggcgaaggc ggaccacgcc gaaaaggcat ccgacgcgga gaa
//
LOCUS       DASWNU010000625         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_101602, whole genome shotgun sequence.
ACCESSION   DASWNU010000625 DASWNU010000000
VERSION     DASWNU010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101602"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VGB57_12455"
     CDS             <1..609
                     /locus_tag="VGB57_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEY0132204.1"
                     /translation="HVSAIGADPESESAYGRSKGEGEEAVRAAFHGATLIRPSLLFGR
                     EDNFVNRFAGMARLSPVLPVVSGRTRLQPVYAADVGRAVTAAALDPRRHGGKTYELGG
                     PQVLTMRELMEWVCETTGRGRPLLDIPDPVGRLIARTTGWLPGAPITWDQWLMLRRDN
                     VVAEGAEGLEAFGLGKTPLSAVSEGWLTSYRRHGRFAAKSPY"
     gene            864..1667
                     /locus_tag="VGB57_12460"
     CDS             864..1667
                     /locus_tag="VGB57_12460"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY0132205.1"
                     /translation="MEASLLTIILLGIVEGVTEFIPVSSTGHLVLAAELLGFDSAAEG
                     TFEIVIQLGAILAVIVVYWRRFLDVLVGLLRWSAGPVAFTRNVLLGFLPSMAIGAVAY
                     EGIQRMLETPEIVAVALIVGGIAILLIERMTRRVTTESVEEMGWKTALGIGLIQCLSM
                     IPGVSRSGATIMGALALGVERKTAAEFSFFVAVPTMIGASAYAMLKSGAKLGAGELGD
                     IAIGFAVSFVVAIVVIKAFIGIVTRYGFAPFAWYRIVIGTVALVWLLAR"
     gene            1864..2385
                     /locus_tag="VGB57_12465"
     CDS             1864..2385
                     /locus_tag="VGB57_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132206.1"
                     /translation="MRKLIIAMTALAAAAPALAQPYHDPRGDDIVRRLPPPHEVERIG
                     DSLGRATDALMDVDIGPVVDALDPYHRRYRRGPQTLGDLAGGRHDPYARERIRGEIGA
                     VTAGLGAAVGQMAVMTPILRRSLEDATRRMEDAIARGRAARSYDRDHDRRYDERYERD
                     YDPRDHDPEYEPR"
     gene            2524..>2911
                     /locus_tag="VGB57_12470"
     CDS             2524..>2911
                     /locus_tag="VGB57_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0132207.1"
                     /translation="MWQLYQFPLCPFSRKVRLMLAEKAIAHELVRESPWLKRDEFTDL
                     NPAGQTPVLVEEARGIVLIDSKAICEYFEETVDKAAMINGTAANRAEIRRLVTWFDTQ
                     FYRDVTGLLLHERVVKRIAQRSAPDAR"
BASE COUNT          434 a          994 c         1007 g          476 t
ORIGIN      
        1 cacgtctcgg cgatcggcgc cgatcccgag agcgaatcgg cttatgggcg aagcaagggg
       61 gaaggggagg aggcggtccg ggccgctttt cacggcgcga ccctcatccg cccctccttg
      121 ctgttcgggc gcgaggacaa tttcgtcaac cgcttcgccg ggatggcgcg gctgtcgccg
      181 gtgcttccgg tggtgagcgg ccgcaccagg ctccagccgg tctatgccgc cgacgtcggc
      241 cgggccgtca ccgccgcggc gctcgatccg cgccggcacg gcggcaagac ctatgagctc
      301 ggcgggccgc aggtcctgac catgcgcgag ctgatggagt gggtttgcga gacgacgggc
      361 cgggggcggc ccctgctcga catcccggat ccggtcggcc ggctgatcgc ccgaaccacc
      421 ggctggctgc ccggggcgcc gatcacctgg gaccagtggc tgatgctgag gcgggacaat
      481 gtcgtcgccg agggcgcgga ggggctggag gcgttcggcc tcggcaagac gccgctctcc
      541 gccgtttccg agggctggct gaccagctat cgccgccacg gccggttcgc ggcgaaatcg
      601 ccttactagc ccgtcatccc ggcgaaggcc gggacccgtg aaaacggaac gtggaaacct
      661 cggactgccg tgttcatgga ttccggcctt cgccggaatg acggggagtg agtcgtcgcg
      721 gcgaagccgg acccggaccg cggcggtgcg gggttccgag cggcggtgtt catggattcc
      781 ggccttcgcc ggaatgacgg gaagggggtt gacccggtct tcccgaacct cgaagcgcgc
      841 accctcaacg ccccggagta acaatggaag cctcgctcct caccatcatc ctgctcggga
      901 tcgtcgaggg cgtgaccgag ttcatcccgg tctcgtcaac cgggcacctg gtcctcgccg
      961 ccgagctgct cggcttcgac agcgcggccg aggggacgtt cgagatcgtc atccagctcg
     1021 gcgccatcct cgccgtcatc gtcgtctatt ggcggcgctt cttggacgtc ctcgtcgggc
     1081 tgctgcgctg gtcggcgggg ccggtcgcct tcacccgcaa cgtccttctc ggcttccttc
     1141 cgtcgatggc gattggggcg gtcgcctatg aagggatcca gcgcatgctg gagactcccg
     1201 agatcgtcgc cgtcgccctg atcgtcggcg ggatcgcaat cctgctgatc gagcggatga
     1261 ccaggcgcgt gactaccgag agcgtcgagg agatgggctg gaagacggcg ctcggaatcg
     1321 gcctcatcca gtgcctgtcg atgatcccgg gggtgagccg ctcgggcgcg acgatcatgg
     1381 gcgcgctggc gctcggggtg gagcgcaaga cggcggccga gttcagcttc ttcgtcgccg
     1441 tgccgacgat gatcggggcc tcggcttatg ccatgctcaa gtccggcgcc aagctcggcg
     1501 cgggggagct gggcgacatc gcgatcggct tcgcggtctc cttcgtggtc gcgatcgtcg
     1561 tcatcaaggc gttcatcggc atcgtcaccc gctacggctt cgcgccgttc gcctggtacc
     1621 ggatcgtcat cgggacggtg gcgctggtgt ggcttctggc gcgctagcgt cattccggcg
     1681 aaagccgcaa tccatgaaca ccgccggtgg gaacgggact cgatccgcgt tcatgggtcc
     1741 cggccttcgc cgggacgacg aatcgcgttc agactaggct cagcctctcc cggccatagc
     1801 ccgcaaccga gtcgcaccct tgcgcgtttt cgccggtgcg ccaatggttt gaggaggcct
     1861 caattgcgta agcttatcat cgccatgacg gcgctcgccg cggccgcgcc cgcactcgcc
     1921 cagccttatc acgatccgcg gggcgacgat atcgtccgcc gactgccgcc gccgcatgaa
     1981 gtcgagcgga tcggcgattc cctggggcgg gcgaccgacg cgctgatgga cgtcgacatt
     2041 ggcccggtgg tcgacgcgct cgacccctat caccgccgct accgccgcgg gccccagact
     2101 ctcggcgacc tcgccggcgg ccgccacgac ccctatgcgc gcgagcggat ccggggcgag
     2161 atcggcgccg tcaccgcggg cctcggcgcc gccgtcgggc agatggcggt gatgaccccg
     2221 atccttcgcc gaagcctcga ggacgccacc cgccggatgg aggacgcgat cgcccgcggc
     2281 cgggcggcgc ggtcctacga ccgcgatcac gaccggcgct acgacgagcg ttacgagcgc
     2341 gactacgacc cgcgcgatca cgatccggaa tacgagccgc gctgaccgtc atcccggcca
     2401 aggccgggac ccatgaacac ggacgccggc ggatgacctc gtccgtcggt gttcatggat
     2461 tccggccttc gccggaatga cggaggtggc gcgcctgtcg taccggcgct agagacgccg
     2521 tccatgtggc agctctacca attcccgctt tgcccctttt cgcgcaaggt ccggctgatg
     2581 ctggccgaga aggcaatcgc gcatgagctg gtgcgcgaat cgccctggct gaagcgcgac
     2641 gagttcaccg acctcaaccc cgccggccag accccggtgc tggtcgagga ggcgcggggg
     2701 atcgtcctca tcgattccaa ggcgatctgc gaatatttcg aggagacggt cgacaaggcc
     2761 gcgatgatca acggcaccgc cgccaatcgc gccgagatac ggcggctggt gacgtggttc
     2821 gacacgcaat tctaccgaga cgtgaccggg ctgctgctcc acgaacgcgt cgtcaagcgg
     2881 atcgcgcagc gctcggcgcc cgacgcgcgc g
//
LOCUS       DASWNU010000626         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_334052, whole genome shotgun sequence.
ACCESSION   DASWNU010000626 DASWNU010000000
VERSION     DASWNU010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334052"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VGB57_12475"
     CDS             <1..150
                     /locus_tag="VGB57_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0132208.1"
                     /translation="RLVGFGTFSCSKRKASTGRNPRTGEPMQIKESTQPKFKAGKVLK
                     DSVGR"
     gene            224..298
                     /locus_tag="VGB57_12480"
     tRNA            224..298
                     /locus_tag="VGB57_12480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:256..258,aa:Val,seq:cac)
     gene            complement(387..593)
                     /locus_tag="VGB57_12485"
     CDS             complement(387..593)
                     /locus_tag="VGB57_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132209.1"
                     /translation="MDDKAKISTGPNERARNESIAGTGPGVPDDARGPGEELPEPPTD
                     EEVERAAEALGVPGRSEEQPLEGQ"
     gene            647..1012
                     /locus_tag="VGB57_12490"
     CDS             647..1012
                     /locus_tag="VGB57_12490"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY0132210.1"
                     /translation="MSEATDAVLRELRAYGLAYPGAHSKAPWPDHDDLAVKDKTFAYL
                     PVAGQAFSLSVKLPYTGEVALDLPYAKPTGYGLGKSGWVSFTPSEAEMPSLEQLKDWI
                     DESYRAQAPKKLVKELDAR"
     gene            complement(1097..2077)
                     /locus_tag="VGB57_12495"
     CDS             complement(1097..2077)
                     /locus_tag="VGB57_12495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0132211.1"
                     /translation="MIPLSVLDLAPVPESADAGRALRNSLELARAAERLGYRRFWMAE
                     HHSMPGIASAATAVALAFVGAGTSTIRIGAGGVMLPNHAPLVIAEQFGTLESLFPGRV
                     DLGLGRAPGTDRAAAYALRRNLASDENQFPNDVVELMAYFEMQEGRAVRAVPGEGLDI
                     PIWILGSSLFGAQLAAMLGLPYAFASHFAPGQMMEAIRVYRERFQPSARLDRPYVMLG
                     FNAFAAETAEEAGLLATSVQQAFVNLRSGNPGKLPPPLPGYAETLPPQARAMLDQVLT
                     CSAIGDAATVKAKIEAFVELTGADELMITSQIYDHGARLRSYEIVAEALA"
     gene            2210..3601
                     /locus_tag="VGB57_12500"
     CDS             2210..3601
                     /locus_tag="VGB57_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0132212.1"
                     /translation="MLQRSPETRRLWRDEIRATLALAWPLVLTNIAQALIHVTDVVLL
                     GWAGPSVLAAGTLGVNVYFAFLIFGIGLVTATAPMIAKELGERRHSVRDVRRTVRQGM
                     WAAVALTIPVWIVLWFSGSILVAIGQDPALAADAQLFVRALQWGFLPYLWYLVLRSFI
                     SALEKPLWSFVVGVVAVLFNGIVNYGLIFGYLGFPKLGLEGAGIGSAMANTLMFVGMA
                     LVVLIHRRFRRFRLFGHFWRSDWLRFREVWRLGLPIAVTLALEITIFNAAVFLMGLIG
                     AESIAAHAIAIQLAALSFMVPMGLSQAATVRVGLAFGRRDRAGIARAGWTAFALGTGF
                     MAVTGLVMLAIPYALVTMFLDPALPANQAVIPLAVSFLIVAAFFQIFDGAQVVAAGML
                     RGLQDTKVPMVYAGIGYWVIGLVTGVGLGFGLGWQGVGIWIGLAAGLAAVAVMLLARW
                     MRRERLGLADWRP"
     gene            3655..3731
                     /locus_tag="VGB57_12505"
     tRNA            3655..3731
                     /locus_tag="VGB57_12505"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3689..3691,aa:His,seq:gtg)
     gene            3760..4806
                     /locus_tag="VGB57_12510"
     CDS             3760..4806
                     /locus_tag="VGB57_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY0132213.1"
                     /translation="MQAPWDYPDFDDHEIVHFVTDSASGLCAVIALHSTYLGPAGGGV
                     RFWHYAEPGEALRDALRLSRGMSFKNAMAGLPLGGGKAVILANESRSKTPEMLAAYGR
                     AVESLGGRYVTAEDVGMSVADMVALSKETSHVAGLPVAEGSVGGDPGPHTSYGVFLGV
                     RAAVKRALGKDSLAGVHIAIQGAGSVASGLARRAAAEGARITLTDIDRGRAEKLAAEV
                     GGEAVEPGSIMTLEADVLSPNALGAILTEESIASLKVPVVAGGANNQLATPEDGQRVH
                     QRGILYAPDYVINAGGIINVSTEYLKDGDQETVRGRIEQIPVRLEQIWTESSATGRNP
                     AEVADAMAMRLIGR"
BASE COUNT          754 a         1735 c         1735 g          835 t
ORIGIN      
        1 cgcctggtcg gtttcggcac tttctcctgc tccaagcgca aggcgtccac cggccgcaac
       61 ccgaggaccg gcgagccgat gcagatcaag gaatccaccc agcccaagtt caaggcgggc
      121 aaggtgctga aggacagcgt cggccgctga ggccgctgca ccgggagctg ccatctggac
      181 agcccgcccg agcgttacta agtggcgcct tcggcaacgg cgtgggcgcg tagctcagtg
      241 gtagagcaca cccttcacac gggtggggtc gtaggttcaa tccctaccgc gcccaccacg
      301 ccttcccggc gaaagccggg accccgggac gagaagtctc ggcgggtctc cccgagatcc
      361 cggccttcgc cgggatgacg cccagcctat tgcccctcca gcggctgctc ctcgcttcgg
      421 cccgggaccc ccagggcctc cgcggcgcgc tccacctcct cgtccgtcgg cggctccgga
      481 agctcctcgc ccgggccgcg cgcgtcgtcg ggaacgccgg gaccggtgcc ggcgatgctt
      541 tcgttgcgcg cccgctcgtt gggccccgtg ctgatcttcg ccttgtcgtc catgccaaac
      601 tctcctcgcc tgtgctaatg acgccgccgc gaggaggttc cgaaggatga gcgaagcgac
      661 cgacgcggtt ttgagggagc ttcgggctta tgggctcgct tatccgggcg cccattcgaa
      721 ggcgccctgg cccgaccatg acgacctcgc cgtcaaagac aagaccttcg cctatctgcc
      781 ggtcgcgggc caggcgttca gcctgtcggt caagcttccc tacaccggcg aggtggcgct
      841 cgatctgcct tatgcgaagc cgaccggata tggcctcggc aaatcgggct gggtgagctt
      901 cacgccttcg gaagcggaga tgccgagcct ggagcagctc aaggactgga tcgacgaaag
      961 ctatcgggcc caggcgccga agaagctggt caaggagctg gacgcgcgtt gaccgtcatc
     1021 ccggcgaaag ccgggacctc ggcgagaccc gccgagcctt ctcgtcccgg ggccccggct
     1081 ttcgccgggg tgacggttac gccaaagcct ccgccacgat ctcatacgac cgcagccgcg
     1141 ccccgtgatc gtatatctgc gaagtgatca tcagctcgtc ggcccccgtc agctcgacga
     1201 acgcctcgat cttcgccttg accgtcgcgg cgtcgccgat cgccgagcag gtgaggacct
     1261 ggtcgagcat cgcgcgtgcc tgcgggggca gggtctcggc atagccggga aggggcggcg
     1321 gcagcttccc cggattgccg ctgcgcaggt tgacgaaggc ctgctgcacc gaggtggcga
     1381 gcaatccggc ttcctccgcc gtctccgcgg cgaaggcgtt gaagccgagc atcacatagg
     1441 gccggtcgag ccgggccgaa ggctggaagc gctcccggta gacccggatc gcctccatca
     1501 tctgcccggg ggcgaaatgg gaggcgaagg cgtagggcag gccgagcatc gccgccagct
     1561 gcgctccgaa caggctcgag cccaggatcc agatcgggat gtcgagcccc tcgccgggaa
     1621 cggcccggac ggcgcggcct tcctgcatct cgaaataggc catcagctcg acgacgtcgt
     1681 tggggaactg gttctcgtcc gacgcgagat tgcggcgcag ggcataagcc gcggcgcggt
     1741 cggtgcctgg agcccggccg aggccgagat cgacccggcc ggggaagagc gactcgagcg
     1801 ttccgaactg ctcggcgata accagagggg cgtggttggg aagcatcact ccgcccgctc
     1861 cgattcggat cgtggaggtg ccggcgccga cgaaggcgag cgcgaccgcg gtcgccgcgc
     1921 tggcgatccc cggcatcgaa tgatgctccg ccatccagaa gcggcgatag cccagccgct
     1981 ccgccgcccg ggcgagctcg agtgagttgc gaagcgcgcg gccggcgtcg gcgctctcgg
     2041 ggactggggc gaggtcgagg actgagaggg ggatcataag cgggatatgg ggacgaggtc
     2101 atcccggcga aagccgggac ctcggggaga cccgccgaga cttcttgtcc tgaggtcccg
     2161 gctttcgccg ggatgacggg ggccccctca gcggctaggg gcacccacca tgcttcagag
     2221 gagccccgag acccgccgac tctggcgcga cgagattcgc gcgacgctcg ctctcgcctg
     2281 gccgctcgtc ctcaccaaca tcgcccaggc gctgatccac gtcaccgacg tcgtcctgct
     2341 cggctgggcg gggccgtcgg tgctggcggc gggcacgctc ggggtgaacg tctatttcgc
     2401 cttcctgatc ttcggcatcg gcctcgtcac cgcgaccgcg ccgatgatcg cgaaggagct
     2461 tggcgagcgc cgccacagcg tccgcgacgt ccgccggacg gtgcgtcagg ggatgtgggc
     2521 ggcggtcgcc ctcaccatac ccgtctggat cgtgctctgg ttcagcggct cgatcctggt
     2581 cgcgatcggc caggatcccg cgctggccgc ggacgcccag cttttcgttc gggcgctgca
     2641 atggggcttt cttccgtacc tctggtacct ggtgctccgt tccttcatct cggcgcttga
     2701 gaaaccgctc tggtcgttcg tcgtcggcgt ggtcgcggtc ctgttcaacg ggatcgtcaa
     2761 ttacggcctg atcttcggct atctgggatt cccgaagctc ggactggagg gggccggcat
     2821 cggcagcgcc atggcgaaca cgctgatgtt cgtcggcatg gcgctggtcg tcttgatcca
     2881 ccgccgcttt cgccgcttcc gactgttcgg gcatttctgg cgttcggact ggctgcgttt
     2941 ccgggaggtc tggcggctcg ggcttccgat cgcggtgacg ctggcgcttg agatcaccat
     3001 cttcaacgcc gcggtcttcc tgatgggcct gatcggcgcc gagtcgatcg ccgcccacgc
     3061 catcgccatc cagctcgcgg ccctgagctt catggtgccg atgggtctgt cgcaggcggc
     3121 gacggtccga gtcggactcg ccttcgggcg gcgcgaccgc gccggaatcg cccgcgccgg
     3181 ctggaccgcc ttcgcgctcg gcaccggctt catggcggtg accggcctcg tcatgctggc
     3241 gatcccctac gcgctggtga cgatgttcct cgatccggcc cttcccgcca accaggcggt
     3301 catcccgctc gccgtctcct tcctgatcgt cgccgccttc ttccagattt tcgacggggc
     3361 ccaggtggtc gcggccggaa tgctgagggg gcttcaggat accaaggtgc cgatggtcta
     3421 tgccggcatc ggctattggg tgatcggcct ggtgaccgga gtcggcctcg gcttcggcct
     3481 gggctggcaa ggggtgggga tctggatcgg cctcgccgcc ggcctcgccg cggtcgcggt
     3541 gatgctgctc gcgcgctgga tgcggcgcga acggctcggt ctcgccgatt ggcgaccttg
     3601 acgctcgggc tccccggccc ctatgtgcgc gccgcacctt gaaggcgcgc agtggcgatc
     3661 gtagctcagt tggttagagc accggtttgt ggtaccgggg gtcgcgggtt caagccccgt
     3721 cgatcgcccc accgcgcctg accagttcag gacatttcaa tgcaagcccc gtgggattat
     3781 cccgatttcg acgatcatga gatcgtccat ttcgtcacgg acagcgccag cggcctgtgc
     3841 gccgtgatcg ccctccactc gacctacctc ggccccgccg gcggcggcgt tcgcttctgg
     3901 cattatgccg agcccggcga agcgcttcgc gacgcgctcc gcctttcgcg cggcatgagc
     3961 ttcaagaacg ccatggcggg ccttccgctc ggcggcggca aggcggtgat ccttgccaat
     4021 gagagccgct ccaagacgcc cgagatgctc gccgcctacg gccgcgctgt cgagagcctg
     4081 ggcggacgct acgtgacggc cgaggacgtc ggaatgagcg ttgccgacat ggtcgccctt
     4141 tcgaaggaga cgagccacgt cgccggcctg ccggtggcgg agggcagcgt cggcggcgat
     4201 cccgggcccc acacaagcta cggcgtcttc ctcggcgtca gggccgccgt gaagcgcgcg
     4261 ctcggcaagg acagcctggc gggcgtgcat atcgccatcc agggcgccgg cagcgtcgct
     4321 tccggcctcg cccgcagggc ggcggcggaa ggagcccgga tcactctgac cgacatcgat
     4381 cgcggccggg cggaaaagct cgcggcggaa gtcggcgggg aggcggtcga gcccggctcg
     4441 atcatgaccc tcgaggccga cgttctcagc cccaacgcgc tcggagcgat cctgaccgag
     4501 gaatcgatcg cttcgctgaa ggtgccggtc gtcgccggcg gcgccaacaa ccagctggcg
     4561 acgcccgagg acggccagcg tgtccaccag cgcggaatcc tctacgcccc cgattacgtg
     4621 atcaacgccg gcgggatcat caacgtctcg accgaatatc tgaaggacgg ggaccaggag
     4681 acggtgcgcg gccggatcga gcagattccg gtccggctcg agcagatctg gaccgagagc
     4741 tccgccaccg gccgcaaccc ggcggaggtc gccgacgcca tggcgatgcg gctgatcggg
     4801 cgctgaccgt cgccccagcg aaggctgggg ccccatgggg tgaagactac tgcgcttctc
     4861 acccgacggg atgccagcct gcgctggcat gacggtgcta cagcgccgcc ggctcccgcg
     4921 ccgcctcgcg cccaagccta tggtcgcggt acaaagtata gagcccgctg gcgatgatga
     4981 tcatcgcgcc gagccaggtg gtggaagcgg gctggtcggc gaaaaccagc cagccgagca
     5041 gcaccgccca gagcaattg
//
LOCUS       DASWNU010000627        13565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_206253, whole genome shotgun sequence.
ACCESSION   DASWNU010000627 DASWNU010000000
VERSION     DASWNU010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206253"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGB57_12515"
     CDS             complement(<1..198)
                     /locus_tag="VGB57_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0132214.1"
                     /translation="MLDFPRWKIWSIVLTVLVGLVFAMPSLMPEATAARIGLGNFPRI
                     NLGLDLAGGSHLLLEADTRDVA"
     gene            complement(207..545)
                     /gene="yajC"
                     /locus_tag="VGB57_12520"
     CDS             complement(207..545)
                     /gene="yajC"
                     /locus_tag="VGB57_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0132215.1"
                     /translation="MFSSPAYAQSGAEAAPGGLSAVLVQFAPLLLIFVIFYFLLIRPQ
                     QKRMKQHRALIDAVKRGDTVVTGGGLIGKATKVDEAEVEIEIAPNVRVKVVKATLSDV
                     RPTGGKPAND"
     gene            complement(649..1128)
                     /locus_tag="VGB57_12525"
     CDS             complement(649..1128)
                     /locus_tag="VGB57_12525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0132216.1"
                     /translation="MDGERRFGVATREEIADLTGLEMLQAIIAGTLPAPPIGATMGFR
                     LVEVEPGRAVFEGSAGPHLLNPLGGVHGGVALTLIDSAAGCAVHTELHAGTGYTTVET
                     KVNFTRPIPPDGSPVRCEGRVVTRGRQIATAEARLLSAEGKVLAHGTSTLIILPPRA"
     gene            1254..1330
                     /locus_tag="VGB57_12530"
     tRNA            1254..1330
                     /locus_tag="VGB57_12530"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1288..1290,aa:Pro,seq:ggg)
     gene            complement(1357..3015)
                     /locus_tag="VGB57_12535"
     CDS             complement(1357..3015)
                     /locus_tag="VGB57_12535"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEY0132217.1"
                     /translation="MEPILLLGLAALLLANLYLFARVNRLEKEIGRVRRGPADLAEPD
                     SGAGQDGAAAPSPPARETLASLFEQLVGGRLLIWIGGIALVVAGFFLIRHTIEIGLVT
                     PELRMAAAALFGVTLLACGEYARNRRFLADEPRVAQALVGAGIAVLYATAYGSWFLYG
                     QIGGKTASALMLLISAAALGLSLRHGPATAVMGMIGGFLTPWLAGDPEGGALPILAYI
                     ALLNAAIFILAWRRGWGWLAAGSVALSFVWTGFLIFGSPPDALAAGLFVVALALVASL
                     LRSGGGRPLALMQPLIIGLVELALLVIRLDTDARAWLLFGALAAIGLWLAARRGEARF
                     APPVGLALALLLLLIKAPIDSDPSVPWTGVAITLMFAGVSIPLSTKDRALRTLTACGA
                     LAGPILILRLLRPELLSPLGWGLAAAALAAAALALLWVHRKDSAGRKMPDSAMVAAGA
                     TAALLLAMAGYDLTARDFVSATWLVLALALVAAGIRIPDKALRLAGLILHTATILKVF
                     LLDAAELRGVLRILSFLGLGIALIGMGKLYGTVLRAERKVRESG"
     gene            complement(3124..3282)
                     /locus_tag="VGB57_12540"
     CDS             complement(3124..3282)
                     /locus_tag="VGB57_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132218.1"
                     /translation="MPTKRRSFFSRLIRALPVVLAAAPGVIDAIQQTRRALREPAAPP
                     RDPALPPA"
     gene            3455..3997
                     /gene="hslV"
                     /locus_tag="VGB57_12545"
     CDS             3455..3997
                     /gene="hslV"
                     /locus_tag="VGB57_12545"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY0132219.1"
                     /translation="MESWHGTTILAVRKNGRAVIAGDGQVTLGQTVIKPNARKVRRLG
                     DGKVIGGFAGATADAFTLFERLERKLEQHHGQLMRAAVELAKDWRTDKYLRNLEAMMI
                     VADKEVTLVLTGNGDVLEPGSGVAAIGSGGNFALAAAIALLDYEEDAEALARKAMGIA
                     AEICVYTNGNLTVESLESAN"
     gene            4057..5292
                     /locus_tag="VGB57_12550"
     CDS             4057..5292
                     /locus_tag="VGB57_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEY0132220.1"
                     /translation="MTGWTVERLDWTDAPLGEIELPKRTMRFRSGFGSGLSRRTGDPA
                     GTVWAVGDRGPNIKVKDVVKLYGLDRLERLRDEDGAKIMPRLDLGPAIAELRVAGDRI
                     EFVRSLSIADSQGRPVSGLPVPGGPHTRCEPAFDLDGEPIAPDPSGLDTEGIAALADG
                     GFWVSDEFGPSLVRLDSGGRVLRRLVPAGTDLAGAAYPVETRFPAIAARRQLNRGFEA
                     IAISPDEKWLFIAFQSPLAHPDEQAHERARHVRLWRLDSETGEVAAQYLYPLDPPESF
                     RRDLEKKPLDRSDLKVSELCWLGADSLLVLERGSETTKIYRVTPSADLALPPAHLDAA
                     TRPTVEELGAGDGDPGLPVLAKHLLFSTDDAPEVGADLEGMAILSPSELLLVNDNDFG
                     VEGAETGFWRVRFDQPLLA"
     gene            5301..6755
                     /locus_tag="VGB57_12555"
     CDS             5301..6755
                     /locus_tag="VGB57_12555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0132221.1"
                     /translation="MPKSRPAFLPLAAGLALLAGCGGGGDYEPEVRAEEKRIGAEQHP
                     QLLAEFGGAYRAPEAVYARRVGQKIAAAAGLEGECTFTLVNSDVVNAFAVPGCYIYVT
                     RGLMGIVNSEAELASVLAHEVGHIVAAHNRRQQRRSLWRSIGVWAVGAITGSEDLTRI
                     AGRAAEYFTLRYSRNQEYEADDLGVRYLAAAGYDPWAAGDMLGALGRHEQYMGRTRGR
                     DEARAIPEWARTHPLAGNRVERAAQTAAATGIADGALPELEGPYLRELDGLLYGDDPA
                     QGFVDGRRFAHPVMRIGFEAPQGFTLTNSPQAILIEGPDGLRGEFGGGPMPPGGLPAY
                     ADGLIRALLRGTPAEAGPAEPALVNGIPALLLPVLVQTEQGPIELSVAAYNGGDGGAY
                     HFIMVGSPAEGARNAVAALFASFRLLSPEQALSLKPRVIRAVAVGPGQDVATFARLMA
                     SDHPLDHFLMLNGRSPGQALKPGELVKIVTFAPR"
     gene            complement(6978..7847)
                     /locus_tag="VGB57_12560"
     CDS             complement(6978..7847)
                     /locus_tag="VGB57_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0132222.1"
                     /translation="MIRPYLPLNGLRAFEAAARHLSFTKAAIELCVTPPALSHQVKAL
                     EERLGVPLFRRLPRGLGLTDEGQALLPVLREAFDRVAAALDGFDGGKGREVLSIGAVG
                     TFAVGWLLPRLAAFRAEHPFVDLRLTTNNNRVDIAEEGLDFAIRFGDGAWHGTDAALL
                     FEAPLTPLCTPRIAARLREPADLAAETLLRSYRVGEWPAWLEAAGVPSPALTGPVFDS
                     AATMIEAALAGHGVALGPACMFERELADGRAARPFRLEVAMGGYWLTSLKSRKPTPAM
                     AAFRSWILAAACE"
     gene            8020..9102
                     /locus_tag="VGB57_12565"
     CDS             8020..9102
                     /locus_tag="VGB57_12565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0132223.1"
                     /translation="MDRRAFIASAASTLVLPGCVTMPRPSAVRSVCLPPVNVAAERVI
                     RTVAGLRPFRASGFVVRAEPLGNKKLVHNYGHGGAGITLSWGSSKLATRLGLPGHAGP
                     VAVIGAGVMGLTTARLVQEAGFAVTIYTEALPPDTTSNIAGGQISPFGHYSEGQVTAE
                     WRAQFAAAMDYSWRRFQIMVGDDYGIHWRPTYQQSSNIARWSDRFHPEARALSPAEHP
                     FPVENLVRYNTMYVETGRFLRELTRDVQIAGGRIEVRKFATPADIAALAERLVFNCTG
                     LGSRELFGDRELHPVRGQLAILLPQPEVRYAFAGQAGHMFPRADGILLGGTFERDEWD
                     ATPQPGDIERIVESHREFFGSFRCTG"
     gene            complement(9190..10338)
                     /locus_tag="VGB57_12570"
     CDS             complement(9190..10338)
                     /locus_tag="VGB57_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY0132224.1"
                     /translation="MALPDANWNFPTAIRFGAGRAAELGEACRGLGVERPLLVTDPAL
                     AKLPMMESALSAAGARLFCDLHANPVGSDVEAGLAAYRDGGHDGVVAMGGGSALDVGK
                     VIAFMSGQSRPLWDFEDKEDWWSRADPAGIAPVVAVPTTAGTGSEVGRAGVILDQDAG
                     VKKIIFHPKMLPAFVILDPELTLGLPPHVTAATGMDALSHCLEAYCAPGFHPMADGIA
                     AEGMKLIREWLPRAVEDGSDIEARGHMLVAASMGATAFQKGLGGMHALSHPIGALLGT
                     HHGLTNAVLMPYMLAFNRAAIEERLARLAGYIGLEDGSFEGFLAWITELRHRLGIPHA
                     LPELGVSAADVERIAIMAEADPSAGGNPLPFDAAAARSVLEAAMAGRV"
     gene            complement(10338..11708)
                     /locus_tag="VGB57_12575"
     CDS             complement(10338..11708)
                     /locus_tag="VGB57_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0132225.1"
                     /translation="MTDLVCLSPVDGREYARRPLATDAEIERALAGARRAQREWAGTP
                     LEERCATMLRFLAAMEALNPDIVPEIAWQMGRPVRYDGEHRSLAERVLAMVDLAPAAL
                     APFVAGAGRSVVRAPAGLVLVIAPWNYPFLTAANTIVPALLAGNAVILKHAAQTPLAG
                     ERFAEALRTAGLPEGLFANLFLSHEQTARLLRSGLADHATFTGSVDGGRAVERAAAGS
                     FTTLTLELGGKDPAYVRGDADVAAAVEGLVDGSFFNSGQSCCGVERIYVHEAVWQPFL
                     DGFAEAASRYRLGDPLDPETSLGPMASAKGAASVRAQVRQAIEAGAAAHLLAQLFPAD
                     TGDGPYLMPQVLTWVNHSMDVMREETFGPAVGIMQVKDDDEAVRLMNDSAYGLTASIW
                     TGDLDVAERLATEVEAGTVFANRCDYLDPLLAWTGLKESGRGASLGRYGFDSVTRPKS
                     LHLRSA"
     gene            complement(11814..13166)
                     /locus_tag="VGB57_12580"
     CDS             complement(11814..13166)
                     /locus_tag="VGB57_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132226.1"
                     /translation="MAKDDLVERVRQRTPACAKVGVFDVDGIFRGKYMAPDKLASALA
                     KGFGFCDVVLGWDSADQLYDNASFTGWHTAYPDAEVRLLPETERAIPFEGGMPLILGE
                     FAGAAEAVCPRGVLRRVLDRAASMGCKVKAAAEFEFFVFEETPHSVRDKGYRGLKPIT
                     PGHFGYSMLRSGVHSDFYDELWSTCDSMRIELEGLHTETGPGVIEGAIRYDDALEAAD
                     KAALFKTIVKILCQRRGWMATFMAKWSNDWPGQSGHLHVSLVDAESGKGRFYDEGEDL
                     HMSQEMRWFVGGQQALMPGLLAMVAPTVNAYTRLIPGFWAPTAALWGYENRTCALRVI
                     GGSDKTQRVEYRIAAADINPYVALAAAIGSGLWGIANHVEPDAPVTGNAYAQEPPPER
                     SLPRTLWEAAQRLRACEAARELFGDAFVDHYAATREWEEREARKAVTDWQLARYFEII
                     "
     gene            complement(13349..>13565)
                     /locus_tag="VGB57_12585"
     CDS             complement(13349..>13565)
                     /locus_tag="VGB57_12585"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125228.1"
                     /note="Conjugates Arg from its aminoacyl-tRNA to the
                     N-termini of proteins containing an N-terminal aspartate
                     or glutamate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY0132227.1"
                     /translation="DDPARPGLGTYIILDHIVRAGKAGLPYVYLGYWVESSPRMAYKA
                     RFRPLERLGPGGWNRFDPDQRELPLSR"
BASE COUNT         2187 a         4714 c         4714 g         1950 t
ORIGIN      
        1 cgccacgtcg cgcgtgtcgg cctcgagcag caggtggctg ccgccggcga ggtcgagccc
       61 gagattgatg cgcgggaagt tgccgaggcc gatccgcgcc gcggtcgcct ctggcatcag
      121 gctcggcatc gcgaagacca ggccgaccag cacggtcagc acgatcgacc agatcttcca
      181 gcgcgggaaa tcgagcatct tggccgtcag tcgttcgcgg gcttgccgcc ggtcggtcgc
      241 acgtcggaca aggtcgcctt gaccaccttc acccgcacgt tgggcgcgat ctcgatctcg
      301 acttcggcct cgtcgacctt ggtcgccttg ccgatcaggc cgccgccggt caccaccgtg
      361 tcgccgcgct tgaccgcgtc gatcaacgcg cggtgctgct tcatcctctt ctgctgcggc
      421 cggatcagca ggaaatagaa gatcacgaag atcagcagca gcggggcgaa ttgcaccagg
      481 acggccgaga ggcctccggg agcggcttcg gcgccggact gggcgtaagc gggcgatgag
      541 aacatgggct atacctggtg agggtccggc gccgacgcaa gcgtccggaa ggaaggtggc
      601 ggcgactaac atgcgttgcg aggccagcgc aagcgcgccg cgagagcttc aggcgcgggg
      661 cggcaggatg atcagggtcg aggtgccgtg ggcgaggacc ttgccctcgg cggagagcag
      721 gcgcgcctcg gccgtcgcga tctgccgccc gcgagtcacc acccgcccct cgcagcggac
      781 gggagagccg tcgggcggga tcggccgggt gaagttcacc ttcgtctcca ccgtcgtgta
      841 gccggttccg gcgtggagct cggtatggac ggcgcagccg gcggcgctgt cgatcagggt
      901 cagcgcgact ccgccatgga cccctccgag cggattgagc agatgcggtc ccgccgaccc
      961 ttcgaaaacc gcccgccccg gctccacctc gaccagccgg aagcccatcg tcgccccgat
     1021 cggcggggcc ggaagcgttc cggcgatgat cgcctgcagc atctcgaggc cggtgagatc
     1081 ggcgatctcc tcccgcgtgg ccactccgaa gcgcctttcg ccgtccatct cccgccctcc
     1141 ttgtcagttg ctattcgcaa ctatatgcgc cgccgcgcaa gtcaacgcga cttgccatcg
     1201 cgcgggccag ggcctaagtc gcgcgccttg ggcgctcgaa acggcgcctc ggtcggggcg
     1261 tagcgcagcc tggtagcgca tcacactggg ggtgtggggg tcgcaggttc gaatcctgtc
     1321 gccccgacca ttttttacaa ggcggcgccc ggccgctcag cccgactccc gcaccttgcg
     1381 ctccgctcga agcacggtgc cgtagagctt gcccatcccg atcagggcga ttcccaggcc
     1441 gaggaacgag aggattcgaa gcacgccccg gagctccgcg gcgtcgagca ggaacacctt
     1501 gaggatcgtc gcggtgtgga ggatcagccc cgccagacgc agcgccttgt ccgggatccg
     1561 gatcccggcc gcgaccaaag cgagcgccag gaccagccac gtcgccgaca cgaagtcgcg
     1621 agccgtgagg tcgtagccgg ccatggcgag aagcaaggcg gcagtggctc ccgcggcgac
     1681 catcgccgag tcgggcatct tgcggccggc tgagtccttc ctgtggaccc acagcaacgc
     1741 gagcgccgcc gccgccagcg ccgcggcggc gaggccccag ccgagcgggg agagcagctc
     1801 gggccgaagc agccgaagga tcagaatcgg cccggccagc gctccgcagg cggtaagcgt
     1861 cctcagggcc cgatcctttg tcgacagcgg gatcgacacg cccgcgaaca tcaaggtgat
     1921 cgcgacgccg gtccacggaa ccgacggatc gctgtcgatc ggcgctttga taaggagcag
     1981 cagcaaggca agcgcaagcc cgacgggcgg cgcgaagcgg gcctctccgc gccgggcggc
     2041 aagccagagg ccgattgccg cgagcgcgcc gaagagcagc caggcgcgcg cgtcggtgtc
     2101 gagccggatc acgagcaaag ccagctcgac gagaccgatg atgagcggct gcatgagcgc
     2161 cagcggccgg cccccgcccg accggagcag cgaagcgacc agggcgaggg cgacgacgaa
     2221 caggccggcg gcgagcgcgt ccggcgggga tccgaagatc aggaagccgg tccagacgaa
     2281 gctcaaagcc accgagccgg ccgcgagcca gccccagccc cgcctccacg cgagaatgaa
     2341 gatcgccgcg ttcaacaaag cgatataggc gaggatcggc agcgctcccc cttcgggatc
     2401 gcccgcgagc cagggcgtca ggaagccgcc gatcatgccc atgaccgccg tcgccggccc
     2461 gtgcctcagc gacaggccca gtgccgccgc gctgatcaga agcatcagcg ccgaagccgt
     2521 cttgccgccg atctggccgt agaggaacca gcttccgtag gccgtcgcgt aaaggaccgc
     2581 gattccggcg ccgaccagcg cctgggcgac ccggggctcg tcggcgagga acctgcggtt
     2641 gcgcgcatat tccccgcacg cgagaagcgt cacgccgaac agcgcggccg ccgccattcg
     2701 aagctccggg gtgacgagcc cgatctcgat cgtatggcgg atcaggaaga atccggcgac
     2761 caccagagcg attccgccga tccagatgag gaggcggccg ccgacgagct gctcgaagag
     2821 gcttgcgagg gtctcccgcg cgggcggcga cggggcggcg gcgccatcct ggccggcacc
     2881 ggagtccggc tcggcgaggt cggccgggcc gcggcgcacc cggccgatct ccttctccag
     2941 ccgattcacc cgcgcgaaga ggtagagatt ggcgaggagc agggcggcga ggccgagaag
     3001 gagaatgggc tccatcgcga ggacgctagc ggcccgccgc tcccctgtca gcgctcaatc
     3061 gcgacaccga agtttctaag tttcggcttc ccaaaggcaa aaaaactttc gccggacgag
     3121 ccctcaagcc ggcggcagcg ccggatcccg gggcggcgcc gcgggctcgc gaagcgcgcg
     3181 ccgggtctgc tggatggcat cgatgactcc gggcgcggcc gcgagcacca ccggcaacgc
     3241 ccttatcaga cggctgaaaa acgaccttct tttcgtcggc atgtctgtcc ctttcgctat
     3301 gtcgcgacga ttcgcgaata ggaaagaaca tagcatgaac aaccttcgta ggaaaatcct
     3361 ttgtgacgcg ccgttgccgg gtcccgacgg tctcgattgc atcgccggcg cccgcctccc
     3421 atatcgccgc cgtccaagag cgggagcatt ttcgatggaa agctggcacg gcacgacgat
     3481 cctggcggtg cgcaagaacg ggcgcgcggt gatcgccggc gacggtcagg tcacgctcgg
     3541 ccagacggtg atcaagccca acgcccgcaa ggtccggcgc ctgggcgacg gaaaggtgat
     3601 cggtggcttc gcaggagcga ccgccgacgc cttcaccctg ttcgagcggc tggaacgcaa
     3661 gctcgagcag catcacggcc agctgatgcg cgcggcggtc gagctcgcca aggactggcg
     3721 gaccgacaaa tatctgagga acctcgaggc gatgatgatc gtcgccgaca aggaggtgac
     3781 cttggtgctg accggaaatg gcgacgttct ggaacccggc tcgggggtcg cggcgatcgg
     3841 ctccggcggc aatttcgcgc tcgccgcggc catcgcgctg ctcgactatg aggaggatgc
     3901 cgaggcgctc gcccgcaagg cgatgggaat cgcggccgaa atctgcgtct acaccaacgg
     3961 caatctgacg gtggagagcc tggagagcgc gaattgaagg caagggattt tcacgtcggc
     4021 atgcgtgact ttcatgaact ttgaccggag cggccgatga cggggtggac ggtcgaacgc
     4081 ctggactgga ccgacgcgcc gctcggcgag atcgagctgc cgaaacggac gatgcgcttc
     4141 cgctccggct tcggctcggg tctttcgcgc cggaccggcg acccggccgg aaccgtctgg
     4201 gcggtcggcg accgggggcc caacatcaag gtgaaggatg tcgtgaagct ctacggcctc
     4261 gaccggctgg agcgcttgcg ggacgaggac ggcgccaaga tcatgcctcg cctcgatctc
     4321 ggccccgcca tcgccgagct gcgcgtggcg ggcgatcgaa tcgagttcgt ccgaagcctg
     4381 agcatcgccg attcgcaggg gcggccggtt tccggcctgc cggtgccggg agggccccac
     4441 acgcgctgcg agccggcctt cgacctggac ggcgagccca tcgcgcccga tccctcggga
     4501 ctggacaccg aaggcattgc cgcgctcgcc gacggcggct tctgggtgag cgacgaattc
     4561 gggccgtcgc tggtccggct cgactccgga gggcgggtgc tcaggcggct ggttccggcg
     4621 ggtaccgacc tggccggcgc cgcctatccg gtggagacgc gctttcccgc catcgcggcg
     4681 cggcgccagc tcaaccgcgg cttcgaggcg atcgcgatct ctcccgacga aaaatggctg
     4741 ttcatcgcct tccagagccc cctcgcccat ccggacgagc aggcccatga gcgggcacgg
     4801 cacgtcaggc tgtggcgcct cgattcggaa accggcgagg tcgccgccca atatctctac
     4861 ccgctcgatc cgccggagag cttccggcgc gatctggaga agaagccgct cgaccggagc
     4921 gacctcaagg tgagcgagct gtgctggctg ggcgccgatt cccttctggt gctcgagcgc
     4981 ggctccgaga ccaccaagat ctaccgggtc accccgtccg ccgatctcgc cctcccgccg
     5041 gcccatctcg acgccgccac tcgcccgacg gtcgaggagc tcggcgccgg cgacggcgat
     5101 cccggcctgc cggtgctcgc caagcatttg ctgttcagca ccgacgacgc ccccgaagtg
     5161 ggcgccgacc tcgaagggat ggcgatcctg tccccgtccg agctgctcct cgtcaacgac
     5221 aatgatttcg gagtcgaagg ggccgagacc ggcttctggc gcgttcgctt cgaccagccc
     5281 cttctcgcct gagccgatac atgccgaaat ctcgccccgc cttcctaccg ctcgccgccg
     5341 gcctcgcctt gctcgccggc tgcggcggcg gcggggatta cgagccggag gtgcgggccg
     5401 aggagaagcg gatcggagcc gagcagcatc cgcagctgct cgcggagttc ggcggcgcct
     5461 atcgggcgcc cgaggcggtc tatgcgcggc gcgtcggaca gaagatcgcc gccgccgcgg
     5521 gcctcgaagg ggaatgcacc ttcacgctcg tcaacagcga cgtcgtcaat gccttcgcgg
     5581 tcccgggctg ctacatctac gtgacccgcg ggctgatggg catcgtcaat tccgaggccg
     5641 aactcgcctc ggtcctggcg cacgaggtcg gccatatcgt cgccgcccac aaccgccggc
     5701 agcagcggcg ctcgctgtgg cggagcatcg gcgtgtgggc ggtgggagcg atcaccgggt
     5761 cggaggacct cacccggatc gccggccggg ccgcggaata tttcaccctc cgctactcgc
     5821 gcaaccagga atatgaagcg gacgatctcg gagtccgcta cctcgccgcc gccggctacg
     5881 atccctgggc ggccggcgac atgctcgggg cgctcgggcg gcacgagcaa tatatgggcc
     5941 ggacccgcgg ccgcgacgaa gcgcgcgcca ttcccgaatg ggcccgcacc cacccgctgg
     6001 ccggcaaccg ggtcgaacgg gccgcccaga ccgcggcggc gacggggatc gccgacggcg
     6061 ccctgccgga gctggaagga ccctatctgc gcgagctcga cggcctgctc tacggcgacg
     6121 atccggcgca gggcttcgtc gacggacgcc gcttcgccca tccggtgatg cggatcggct
     6181 tcgaggctcc gcaaggcttc accctgacca acagcccgca ggcgatcctg atcgagggac
     6241 cggacggcct tcgcggcgag ttcggcggcg gtccgatgcc gccgggcggc cttccggcct
     6301 atgccgacgg cctgatcagg gcgcttcttc gcgggacccc ggccgaggcg ggcccggccg
     6361 agccggcgct ggtgaacgga atccctgccc tgctcctgcc cgtcctggtg cagaccgagc
     6421 aggggccgat cgagctttcc gtggccgcct acaatggcgg cgacggcggc gcctatcatt
     6481 tcatcatggt cgggagcccc gccgaagggg ctcgaaacgc agtcgccgcc ctcttcgcgt
     6541 cgttccgcct gctctcgccc gagcaggcgc tcagcctgaa gccgcgggtg atccgggcgg
     6601 tggcggtggg gccggggcag gatgtcgcga ccttcgcccg actgatggcg agcgatcacc
     6661 cgctcgacca tttcctgatg ctcaacggac gctctcccgg ccaggcgctg aagcccggcg
     6721 aattggtcaa gatcgtcacc ttcgcgccgc gctgagggcg aaggggggca ggcgccatcc
     6781 gtgcctgacc tccgacggcg ggtcctgccg gcagattcaa cgcaacgact cgacgacgca
     6841 acgcgcgcgg agcgatccct gatcagagcg tgtccgggga cgcgacgggt tgatccgctt
     6901 cgcggagtcg ttgcgccgtt gcgttcaaag atgggctcga atcgcgcccc gggatatgtc
     6961 gcccggtgga gcgccactca ctcgcaagcg gcggcgagga tccaggagcg gaatgccgcc
     7021 attgcgggag tcggcttccg ggacttgagg ctggtcagcc agtagccgcc catggcgacc
     7081 tccaggcgga acggccgggc cgcccggcca tcggcaagct cgcgctcgaa catgcaggcg
     7141 ggcccgagcg cgactccgtg cccggcgagc gccgcttcga tcatcgtcgc ggcgctgtcg
     7201 aagaccgggc cggtcagcgc cggcgagggc acgcccgccg cctcgagcca ggcgggccat
     7261 tcgcccacgc ggtaggagcg gagcagggtc tcggcggcga gatcggccgg ctcgcgcagc
     7321 cgggccgcga tccgcggcgt gcagagcggg gtcagcggcg cctcgaaaag gagcgcggca
     7381 tcggtgccgt gccaggcgcc gtcgccgaac cttatcgcga aatccagccc ctcctccgcg
     7441 atgtcgaccc ggttgttgtt ggtggtcagg cggaggtcga cgaagggatg ctccgctcgg
     7501 aaggccgcga gccggggcag cagccagccc accgcgaacg taccgaccgc gcctatcgaa
     7561 agcacctctc gccccttgcc gccgtcgaac ccgtccagcg ccgcggccac ccggtcgaac
     7621 gcctcgcgaa gcaccgggag cagcgcctgg ccctcgtcgg tgagcccgag cccgcgcggc
     7681 agccggcgga acaacggcac gcccagcctt tcctccagcg ccttgacctg gtggctgagc
     7741 gcgggcggag tcacgcacaa ttcgatcgcc gctttggtga agctcaggtg acgcgccgcc
     7801 gcctcgaagg cgcgaaggcc gttcaggggc agataggggc ggatcatccc ttgcttctaa
     7861 ccgggcgggg catggaggta agatgaagaa atctatcggc tgccgctagc tatcctcgtt
     7921 tgcgccggcg gcggcgggcg gcgacaagct tcgccacgct gcaaagttca gcaatttgag
     7981 ggctcccggt ggcttgcgcg aatgattagg ctggacgcaa tggaccgccg cgccttcatc
     8041 gcctcagccg cttcgaccct cgtcctgccg ggttgcgtga cgatgccccg tccttcggcg
     8101 gtccgctccg tctgcctgcc cccggtcaac gtcgcggccg agcgggtcat ccgcaccgtc
     8161 gccggccttc gccccttccg ggcttcgggc ttcgtcgtcc gggcggagcc gctgggcaac
     8221 aagaagctgg tccacaatta cggccatggc ggcgccggga tcaccctgag ctggggcagc
     8281 tcgaagctcg cgacgcggct cggccttccg ggccatgccg ggccggtcgc ggtgatcggc
     8341 gccggggtga tgggcctgac caccgcacgg ctcgtccagg aggcgggatt tgcggtgacg
     8401 atctacaccg aggcgctgcc gcccgacacg acctccaaca tcgcaggcgg ccagatctcg
     8461 cccttcggcc attatagcga gggccaggtg accgccgaat ggcgggcgca gttcgcggcg
     8521 gcgatggact atagctggcg gcgcttccag atcatggtgg gcgacgatta cggcatccac
     8581 tggcggccga cctaccagca atcgagcaat atcgcccggt ggtcggatcg ctttcatccc
     8641 gaggcccgcg ccctgagccc ggccgagcat cccttcccgg tcgagaacct cgtccgctac
     8701 aacaccatgt atgtcgagac cggccgcttc cttcgcgagc tgacccggga cgtgcagatc
     8761 gccggaggcc ggatcgaggt ccgcaagttc gcgaccccgg cagatatcgc ggcgcttgcc
     8821 gagcggctcg tgttcaactg caccggcctc ggctcccgag agctgttcgg cgacagggaa
     8881 ttgcatccgg tacgaggcca gctcgccatc ctgcttccgc agccggaggt gcgctacgcg
     8941 ttcgccgggc aggcgggcca catgttcccg cgcgccgacg ggatactgct cgggggcacg
     9001 ttcgagcgcg acgaatggga cgccacgcct cagcccgggg atatcgagag gatcgtggag
     9061 tcgcacaggg aattcttcgg cagcttccgc tgcacgggct aagcctcaca ccggcggaag
     9121 ccgggacatc ggggagactc gccgagaccc ttttgtcctg aggtcccggc cttcgccggg
     9181 atgacggggt cagacccgtc ccgccatcgc cgcctcaagc accgaccgcg ccgcggcggc
     9241 gtcgaagggg agcggattgc caccggcgga aggatcggcc tcggccatga tcgcgatccg
     9301 ctcgacgtcg gccgccgata caccgagctc cggcagggcg tggggaatgc cgaggcggtg
     9361 gcggagctcg gttatccagg ccaggaagcc ttcgaagctg ccgtcctcca agccgatata
     9421 gcccgcgagc cgggccagcc gctcctcgat cgcggcccgg ttgaaggcca gcatgtaggg
     9481 catcagcacc gcgttggtga ggccgtgatg ggtgccgagc agcgccccga tcggatgcga
     9541 cagagcgtgc attccgccga ggcccttctg gaaggcggtc gcgcccatcg acgcggcgac
     9601 gagcatatgg ccgcgggcct cgatgtcgga cccgtcctcc accgcgcgag gcagccactc
     9661 tcggatcagc ttcatcccct ccgccgcgat cccgtcagcc atcgggtgga agccgggcgc
     9721 gcaataagcc tccaggcagt gcgaaagcgc gtccatgccg gtggcggctg tcacgtgcgg
     9781 cggcaggccc agggtgagct cggggtcgag gatcacgaag gccggcagca tcttcgggtg
     9841 gaagatgatc ttcttcacgc cggcatcctg atcgaggatc actccggcgc ggccgacctc
     9901 ggagccggtc ccggccgtcg tcggaaccgc gacgacgggc gcgatcccgg ccggatcggc
     9961 tctggaccac caatcttcct tgtcctcgaa atcccagagc ggccggcttt ggccggacat
    10021 gaaggcgatc accttgccga cgtcgagcgc gctgcctccg cccatggcga ccactccgtc
    10081 gtggccgccg tccctgtagg cggcgaggcc tgcctccaca tccgagccga cggggttggc
    10141 gtggaggtcg cagaacagtc gggcaccggc ggcggagagg gcggactcca tcatcggcag
    10201 cttcgccagg gccggatcgg tgacgagcag cggccgctcg acgccgagcc cccggcacgc
    10261 ctcgcccagc tcggcggccc ggcccgcgcc gaagcggatc gccgtcggaa aattccagtt
    10321 ggcgtcgggc aaggccatca ggcgctccgc agatgaaggg atttgggccg ggtgaccgaa
    10381 tcgaagccgt agcgcccgag cgaggcgccg cggccgctct ccttgagccc ggtccaggcg
    10441 agcagcgggt cgagatagtc gcagcggttg gcgaagacgg tccccgcctc gacctcggtc
    10501 gcgagcctct cggcgacatc gaggtcgccc gtccagatcg aggcggtgag gccgtaggcg
    10561 ctgtcgttca tcaggcgcac cgcttcgtcg tcgtccttga cctgcatgat cccgaccgcc
    10621 gggccgaacg tctcctcgcg catgacgtcc atcgaatgat tcacccaggt cagaacctgc
    10681 ggcatcagat agggtccgtc gcccgtgtcg gcggggaaga gctgcgccag gaggtgcgcg
    10741 gccgcgcccg cctcgatcgc ctggcgcacc tgcgcccgga ccgaagcggc gcccttcgcg
    10801 ctcgccatgg gccccaggct ggtctccggg tcgagcggat cgccgaggcg gtagcgcgac
    10861 gcggcttcgg cgaagccgtc gaggaaaggc tgccacaccg cctcgtgcac gtagatgcgc
    10921 tcgaccccgc agcaggactg gccgctgttg aagaagctgc cgtcgacgag cccctcgacc
    10981 gcggccgcca cgtccgcatc cccgcgcaca taagcgggat ccttgccgcc gagctccagg
    11041 gtcagggtgg tgaacgaccc cgccgccgcc cgctcgaccg ccctgccgcc gtcgacggat
    11101 ccggtgaagg tggcgtgatc ggccaggccc gagcggagca gccgcgccgt ctgctcgtgg
    11161 ctgaggaaca ggttggcgaa gagcccctcg ggcagtccgg cggttcgaag cgcctcggcg
    11221 aagcgctcgc ccgccagcgg agtttgagcg gcgtgcttga ggatgacggc gttgccggcg
    11281 aggagcgccg gcacgatcgt attggcggcg gtgaggaagg ggtagttcca gggcgcgatc
    11341 acaagcacca ggccggcggg cgcccgaacc accgaccggc cggcgcccgc gacgaacggc
    11401 gcgagcgccg ccggcgccag gtcgaccatc gccaggaccc gctccgccag cgagcgatgc
    11461 tccccgtcgt agcggaccgg gcggcccatc tgccaggcga tctcggggac gatgtcgggg
    11521 ttgagggcct ccatcgcggc gaggaagcgg agcatcgtcg cgcagcgctc ctcgagcgga
    11581 gtcccggccc attcgcgctg ggcccgccgg gccccggcca gcgcgcgctc gatctcggcg
    11641 tcggtcgcca acggccgccg cgcatattcg cggccgtcga cgggcgagag gcagaccaga
    11701 tccgtcatgg gttcccgtca ttgcgaggag cgaagcgacg aagcaatcca tcgggtggcg
    11761 gcgcagcact ggattgcttc gctgcgctcg caatgacggt ttccgctccc acctcagatg
    11821 atctcgaaat agcgggcgag ctgccagtcg gtcaccgcct tgcgggcctc gcgctcctcc
    11881 cattcgcggg tggcggcata atggtcgacg aaggcgtcgc cgaacaactc gcgcgccgcc
    11941 tcgcacgctc gaagccgctg ggcggcttcc cacagggtcc ggggcagcga ccgctcaggc
    12001 ggaggctcct gcgcataggc gttgccggtc accggcgcgt ccggctccac atggttggcg
    12061 atgccccaga ggccggagcc gatcgccgcg gcgagcgcga cgtaggggtt tatgtcggcg
    12121 gcggcgatcc ggtactcgac ccgctgggtc ttgtccgacc cgccgatgac tcgaagcgcg
    12181 caggtgcggt tctcatagcc ccaaagggcc gcggtcggag cccagaagcc cgggatgaga
    12241 cgggtatagg cgttgaccgt cggagcgacc atcgcgagca gcccgggcat cagcgcctgc
    12301 tgcccgccga cgaaccagcg catctcctgc gacatgtgga gatcctcgcc ctcatcgtag
    12361 aagcgcccct tcccgctctc cgcgtcgacc agcgacacat ggagatgccc ggactggccc
    12421 ggccagtcgt tcgaccattt cgccatgaac gtcgccatcc agccgcggcg ctggcacaat
    12481 atcttgacga tcgtcttgaa cagggccgcc ttgtccgccg cctccagagc gtcgtcgtag
    12541 cggatcgcgc cctcgattac gcccggcccg gtctcggtgt gaagcccctc aagctcgatc
    12601 cgcatcgagt cgcaggtcga ccagagctcg tcatagaagt ccgaatgcac gccggagcga
    12661 agcatcgagt agccgaaatg gcccggcgtg atcggcttca gaccgcgata gcccttgtct
    12721 cgcacggaat ggggagtctc ctcgaagacg aagaattcga actccgcggc cgccttgacc
    12781 ttgcacccca tcgaggcggc gcgatcgagc acccggcgaa gcaccccgcg cgggcagacg
    12841 gcctcggccg cgccggcgaa ctcgccgagg atgagcggca tccccccttc gaacggaatc
    12901 gcccgctcgg tttcgggcag cagccgcacc tcggcgtcgg gataagcggt gtgccagccg
    12961 gtgaagctgg cattgtcgta aagctggtcg gcggaatccc agcccagcac cacgtcgcaa
    13021 aagccgaagc ccttggcgag ggcggaagcg agcttgtcgg gcgccatata tttgccgcgg
    13081 aagatgccgt cgacgtcgaa cacgcccacc ttcgcgcagg cgggcgtgcg ctgacggact
    13141 cgctcgacca ggtcgtcctt cgccatgctt cgcgcctctt tccgtggtgg ccgagccatc
    13201 ggtaacgcct gaggcggcgc ggcggaagca cagacgcgcc tgccgccgga gccgatggag
    13261 tcgtcatccc ggcgaaagcc gggatctcgg gacgagaagt ctcggcgggt ctccccgagg
    13321 tcccggcttt cgccgggacg acggggtttc agcgcgacag aggaagctcg cgctgatcgg
    13381 ggtcgaaccg gttccagccg ccgggaccga gccgctcgag cggccgaaag cgcgccttgt
    13441 aggccattcg cggcgagctt tcgacccaat agccgagata gacgtagggg aggccggcct
    13501 tcccggcccg gacgatatgg tcgaggatga tgtaggtgcc gaggcccggc cgcgccggat
    13561 cgtcg
//
LOCUS       DASWNU010000628         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_464858, whole genome shotgun sequence.
ACCESSION   DASWNU010000628 DASWNU010000000
VERSION     DASWNU010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464858"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /gene="pyrC"
                     /locus_tag="VGB57_12590"
     CDS             complement(<1..865)
                     /gene="pyrC"
                     /locus_tag="VGB57_12590"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0132228.1"
                     /translation="MTELTRLTLRCPDDWHVHLRDGAMLEAVAPYTARQFARAIVMPN
                     LETPVTTAAMALAYRERILAAAPGLTPLMTCYLTDDSDPAELTGGFERGSWVAAKLYP
                     AHATTNSAHGVTDIRNIHPVLEAMQRAGMPLLIHGEVTDPEVDVFDREKAFIDRIFSR
                     LLRDFPGLKMVFEHITTAEAADFVMAGGDNLAATITPQHLIINRNALFAGGLRPHAYC
                     LPVAKREEHRRAIRKAATSGSPKFFLGTDSAPHSIERKESGCGCAGIFNAPFALESYA
                     TVFEEEGSLDRL"
     gene            1014..1745
                     /locus_tag="VGB57_12595"
     CDS             1014..1745
                     /locus_tag="VGB57_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY0132229.1"
                     /translation="MAATTLTDRALLRLSGEDVRGFLQGLVTHDMVLLGPQRPLWAGL
                     LTAQGKALFDFILWADGEDVLIDCEAEAAGDLARRLTLYRLRRKIAIEPVEGLAVHWA
                     QEGDGLPDPRLPTLGRRWLAPVGDPAQGWLARRLSLGVTEGRAELGSDRILWLECNAA
                     ELNGVSFTKGCYVGQENTARMNYRSKVNRRLVVVPTDEPGPRTRIHYSGLGLAVEHRR
                     VDDLGAALVPEWLAPSLATDAIPSP"
     gene            complement(1836..2354)
                     /locus_tag="VGB57_12600"
     CDS             complement(1836..2354)
                     /locus_tag="VGB57_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0132230.1"
                     /translation="MLQPFFAALALAAANPAPAAPSADVAMPAPLNPQIDYEGYRALI
                     AEVQPYRRARLIDWKAFTAAAAEPDVLILDARSAGQFAAGHIRGAVNVPLPEFSDERL
                     AGAIGRADRPILIYCNNNFRNDRPPVMLKTGRLALNLSTFTHLYGYGYRNVRELNDVM
                     DFDDPKVRWVKG"
     gene            complement(2430..3074)
                     /locus_tag="VGB57_12605"
     CDS             complement(2430..3074)
                     /locus_tag="VGB57_12605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0132231.1"
                     /translation="MLIDLSHDIEDGMVTYKGLPAPHICDFWKREDTGASYDDGSTFQ
                     IGRIDMVANTGTYLDTPFHRYADGDDLAAVDLERLAGLRALCVRAEGMEAGAELFEGL
                     DVAGRAVLVHTGWDRHWRTDAYHSDHPFLAEAAARLLAERGAALVGIDSYNIDDTRTR
                     RRPVHTILLGAGTLICEHMTRLGELPTGGFRFTAAPPRVAGMGTFPVRAFAEIG"
     gene            complement(3058..3390)
                     /locus_tag="VGB57_12610"
     CDS             complement(3058..3390)
                     /locus_tag="VGB57_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132232.1"
                     /translation="MIELLLLAMLAKAEPKYEPPPAVFYPQAAGCAATTFIAKSKEPT
                     GDEFGQSMTWGMIVADMGKKAGRTAAQVESETEAAIGFFRKLKEMKPPAFAAHLAYCR
                     AVLDADRP"
     gene            complement(3387..3638)
                     /locus_tag="VGB57_12615"
     CDS             complement(3387..3638)
                     /locus_tag="VGB57_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132233.1"
                     /translation="MEIAGWAGALLILLAYALLSLGRLTGQSALYQGINVVGAAGFIV
                     NGWWHGAIPSASLNVVWMLIGGFALLRILRRRRTEGDSS"
     gene            complement(3656..>4118)
                     /locus_tag="VGB57_12620"
     CDS             complement(3656..>4118)
                     /locus_tag="VGB57_12620"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051759.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY0132234.1"
                     /translation="EGGLARHIPTGETMPIYDAAMRYKAEGTPLVVIAGKEYGTGSSR
                     DWAAKGTNLLGVRAVIAETYERIHRSNLVGMGVVPLQFVGDVPIFDGTETFTINGIAD
                     LQPRQETEVRVTRADGSAFAFAARVRIDTLNELEYFRAGGILHYVLRKLAA"
BASE COUNT          645 a         1323 c         1485 g          665 t
ORIGIN      
        1 ccaggcggtc gagcgagccc tcctcctcga acaccgtcgc gtagctctcc agagcgaagg
       61 gcgcgttgaa gatcccggcg cagccgcagc ccgattcctt acgctcgatg ctgtgcgggg
      121 cgctgtcggt gccgaggaag aatttgggcg agcccgacgt cgccgccttg cggatcgcgc
      181 ggcgatgctc ctcgcgcttg gcgaccggca ggcaataggc atgcggcctc aaaccgccgg
      241 cgaacagggc gttgcggttg atgatcagat gctgcggcgt gatcgtcgcg gcgagattgt
      301 cgccgccggc catgacgaaa tccgcggcct cggcggtggt gatatgctcg aacaccatct
      361 tgaggcctgg aaagtcgcgc agcagccgcg agaagatgcg gtcgatgaag gccttctccc
      421 ggtcgaagac gtcgacctct ggatcggtga cctcgccgtg gatcagaagc ggcatgcccg
      481 cgcgctgcat cgcctccagc accggatgga tgttcctgat gtcggtgacc ccatgcgcgc
      541 tattggtggt ggcgtgggcc ggatatagct tggccgccac ccaggagccg cgctcgaagc
      601 cgccggtcag ctccgccggg tccgaatcgt cggtgaggta gcaggtcatc agcggggtta
      661 ggcccggcgc cgcggcgagg atccgctccc ggtaggccag cgccatcgcg gccgtggtga
      721 ccggagtctc caggttcggc atcacgatcg cccgggcgaa ctgccgggcg gtgtaaggcg
      781 ccaccgcctc gagcatggcg ccgtcgcgca ggtggacgtg ccagtcgtcc gggcagcgga
      841 gggtgaggcg ggtgagttcg gtcatgtttc ctcagccgtc atcctgaact tgtttcagga
      901 tccagatcga tgaaggtcgg cgatgctcga aactggaccc tgaaacaagt tcagggtgac
      961 ggcctggcgc cgggacggtt ggaggcaggg gcacaaacgc ctagatgagt ctcatggccg
     1021 ccaccaccct caccgaccgc gccctgctcc gcctctccgg cgaggacgtg cgcgggttcc
     1081 tgcagggact cgtcacccac gacatggttc tgctcggccc gcaacggccg ctctgggcgg
     1141 ggctgctgac ggcgcagggc aaggcgctgt tcgatttcat cctgtgggcg gacggcgagg
     1201 acgtgctgat cgactgcgaa gccgaggcgg ccggcgacct tgcgaggcgc ctgacgctct
     1261 accggctgcg gcggaaaatc gcgatcgagc cggtggaggg gctcgcggtc cattgggcac
     1321 aggagggcga tggccttccc gaccctcggc tgccgacgct cggccgccgc tggctggccc
     1381 ccgtcggcga tcccgcgcaa ggctggctcg cccgccggct ctcgctgggc gtgacggagg
     1441 ggcgggcgga gctcggctcc gacaggattc tctggctcga gtgcaacgcg gccgagctca
     1501 acggcgtgag cttcaccaag ggctgctatg tgggccagga gaatacggcc cggatgaact
     1561 accggtccaa ggtcaaccgg cggctggttg tcgtgccgac ggacgagccc ggcccgcgaa
     1621 cccggatcca ctattccggg ttggggctgg cggtggagca tcggcgggtc gacgatctcg
     1681 gcgcggcttt ggtgccggag tggctggcgc cgtcgctcgc gaccgacgcc atcccctcac
     1741 cctgaacttg cttccgggtc caggtcccca tccgccccgc ggtctccgga ctggatgctg
     1801 aagcaagttc agcatgacgg ggggtcggcg caagctcagc ctttcaccca ccgcaccttc
     1861 ggatcgtcga agtccatcac gtcgttcagc tcgcgcacgt tgcggtagcc gtagccgtaa
     1921 aggtgggtga aggtcgaaag gttgagtgcg agccgtccgg tcttgagcat gacgggaggg
     1981 cgatcgttgc ggaaattgtt gttgcagtag atcaggatcg ggcgatccgc ccgtccgatc
     2041 gccccggcga gacgctcgtc gctgaactcg ggcaggggca cgttgacggc gccccttatg
     2101 tggccggccg cgaactgccc ggcggagcgg gcgtcgagga tcagcacatc cggctcggcc
     2161 gcggcggcgg tgaaagcctt ccagtcgatc aggcgagccc ggcgataggg ctgaacctcc
     2221 gcgatcaagg cacgatagcc ctcataatcg atctgcgggt tgagcggcgc gggcatcgcc
     2281 acgtccgccg agggcgcggc gggggcagga ttggcggcgg cgagggccaa agcggcgaag
     2341 aaaggctgga gcatggcggg cactccttac tgacaggcag cgggggagca tatcgatacg
     2401 gcagcgaaac tgtcgccgcc gtgaacgctt cagccgatct ccgcgaaggc cctgaccggg
     2461 aaggtgccca ttccggcgac cctcggcggc gccgcggtga agcgaaagcc gcccgtcggc
     2521 agctcgccga gcctcgtcat atgctcgcag atcaaagtgc cggcgccgag caggatggtg
     2581 tggacggggc ggcggcgggt gcgcgtgtcg tcgatattgt agctgtcgat cccgaccagg
     2641 gcggcgccgc gctccgccag caggcgggcg gcggcttcgg cgaggaacgg gtggtccgaa
     2701 tgataagcgt cggttcgcca gtgtcggtcc cagccggtgt ggacgagcac cgccctgccg
     2761 gccacgtcga ggccctcgaa cagttccgct ccggcctcca ttccttcggc gcggacgcag
     2821 agggcccgca gcccggcgag gcgctccagg tcgaccgccg cgaggtcatc gccgtcggcg
     2881 tagcggtgga agggcgtgtc gagataggtg ccggtattgg cgaccatgtc gatccggccg
     2941 atctggaagg tcgagccgtc gtcgtagctc gctccggtgt cctcgcgctt ccagaagtcg
     3001 cagatgtgcg gcgcgggcag gcccttgtag gtgaccatgc cgtcttcgat gtcgtggcta
     3061 aggtcgatca gcatcgagca cggcccggca ataagcgagg tgggcggcga aggcgggagg
     3121 cttcatctcc ttgagcttgc ggaagaagcc gatggccgct tccgtctcgc tctcgacctg
     3181 ggcggcggtg cggccggcct ttttgcccat gtccgcgacg atcatgcccc aggtcatcga
     3241 ctggccgaac tcgtcgccgg tgggctcctt gctcttggcg atgaaggtcg tggccgcgca
     3301 gccggcggcc tggggataga agaccgcggg cggcggctcg tacttcggct ccgccttggc
     3361 gagcatggcg agcagcagca gttcgatcat gacgaatccc cctccgtccg gcggcgcctg
     3421 aggatgcgca ggagcgcgaa gccgccgatc agcatccata ccacgttgag cgacgccgac
     3481 ggaatggccc cgtgccacca gccgttgacg atgaagccgg cggccccgac gacgttgatc
     3541 ccctggtaga gcgccgactg gccggtcagc cgccccagcg agagcaaggc gtaagcgagc
     3601 aggatcagca gggcgcccgc ccagccggcg atctccaccg cgatctcgat cccgctcagg
     3661 cggcgagctt ccgaagcacg tagtggagga tcccgccggc acggaaatat tcgagctcgt
     3721 tcagggtatc gatccggacc cgggcggcga aggcgaaggc ggagccgtcg gcccgggtca
     3781 cccggacctc tgtctcctga cgcggctgga ggtcggctat cccgttgatg gtgaaggtct
     3841 cggtaccgtc gaagatcggg acgtcgccga cgaactggag cggcaccacg cccatcccga
     3901 ccaggttgga gcggtggatc ctctcgtagg tctcggcgat gacggcgcga acgccgagca
     3961 ggttggtgcc cttggcggcc cagtcgcgcg aggagccggt gccatattcc ttgccggcga
     4021 tgacgacgag cggggtcccc tccgccttgt agcgcatcgc cgcgtcgtag atgggcatcg
     4081 tctcgccggt cggtatgtgg cgggccaggc ccccttcc
//
LOCUS       DASWNU010000629         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_206386, whole genome shotgun sequence.
ACCESSION   DASWNU010000629 DASWNU010000000
VERSION     DASWNU010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206386"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /gene="purL"
                     /locus_tag="VGB57_12625"
     CDS             complement(<1..1615)
                     /gene="purL"
                     /locus_tag="VGB57_12625"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY0132235.1"
                     /translation="MTSSITADVVAEHGLSPEEYDRVLHALGREPNLTELGIFSVMWS
                     EHCSYKSSRIHLKKLPTTAPWVICGPGENAGVIDIGDNQAAIFKMESHNHPSYIEPYQ
                     GAATGVGGILRDVFTMGARPVANMNALRFGRPDHPKMKHLIAGVVAGIGGYGNCVGVP
                     TVGGEVNFHPAYDGNILVNAMTVGIAEQDKIFYSAAAGIGNPVVYVGSKTGRDGIHGA
                     TMASADFSEDSEEKRPTVQVGDPFTEKLLIEACLELMASDAIVAIQDMGAAGLTSSSV
                     EMASKGGVGLELDMNAVPCREEGMTPYEMMLSESQERMLMVLKPGREAEAEAIFRKWE
                     LDFAVIGQVTDTGHMVLKFNGEVAADIPLAPLADDAPCYDRPWVPTPKRAPLADVPES
                     ADIAADLLKLMASPDLASRRWIWEQYDYGVGADTVQPPGGDAAVVRVHGTDKALAMTC
                     DVTPRYCYADPVEGGKQAIAEAYRNLCAVGARPLAATDCMNFGNPQRPEIMGQFVGCI
                     AGMAEACRALDFPIVSGNVSLYNETKGEDG"
     gene            1711..2175
                     /locus_tag="VGB57_12630"
     CDS             1711..2175
                     /locus_tag="VGB57_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0132236.1"
                     /translation="MALNPIPEGYHSVTPYLIVDGAAEAIRFYGEAFGAAEVLRMPMG
                     DRIAHAEIRIGDSHVMLSDEMPDMGFLGPKTRGGPTASLMIYLADVDAAFERAVAAGC
                     TEERPPADQFWGDRMGTLLDPFGHRWTLATHVEDVSEEEMDRRMKEAFCGAG"
     gene            complement(2383..2772)
                     /locus_tag="VGB57_12635"
     CDS             complement(2383..2772)
                     /locus_tag="VGB57_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132237.1"
                     /translation="MARNLISFAAALVAGSLALSACNTEPETVGPADNDPQRDALAKA
                     APKELPPSIQASRTYRCQDNSLVYVDFYTNNTAQVRTTEGGTPTMLRAEGGNPPYVAE
                     GHSVAANAERTNIAVPGKGSQSCRARG"
     gene            2917..3183
                     /locus_tag="VGB57_12640"
     CDS             2917..3183
                     /locus_tag="VGB57_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132238.1"
                     /translation="MQRVRIGLTGLAFVFLLVVLATILVRPSEEPPLSANTVGQPRGA
                     TGSTANSSGEPTEPLAQLGVAPGSAETNNSAAPSAPPVAPAPAR"
     gene            3161..>3862
                     /locus_tag="VGB57_12645"
     CDS             3161..>3862
                     /locus_tag="VGB57_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132239.1"
                     /translation="MPPPRLAKGAAAPLIGAGLFGACELAAGLLVGRFFEWGRAEALL
                     FLAFRPWLLVVGAALASTWRWRQRAVFYTAALVAAGSAETILLASLGAADPWPELLRG
                     LAGGAAVALVADIAVQTAGRWRPRLGALLAAAVLIALLAAGAIRPYEALVLGPTVPPA
                     QTGPKPPLLLMTSLPLVWGPGGAFDPESRPSESYRALGREYDVRPIDAIEPGSLAGAR
                     LMLLAQPRLLAPEELV"
BASE COUNT          534 a         1284 c         1386 g          658 t
ORIGIN      
        1 tgccgtcctc acccttggtc tcgttgtaga gcgagacgtt gccggagacg atcgggaagt
       61 cgagcgcgcg gcaggcttcg gccatgccgg cgatgcaccc cacgaactgg cccatgatct
      121 cgggtcgctg cgggttgccg aaattcatgc agtcggtcgc cgcgagcggg cgggcgccga
      181 ccgcgcaaag attgcggtac gcttcggcga tcgcctgctt cccgccctcg acgggatccg
      241 cgtaacaata acgcggcgtg acgtcgcagg tcatggcgag cgccttgtcg gtgccgtgga
      301 cgcgcaccac ggcggcgtcc ccgcccggcg gctgcaccgt gtcggcgccg acgccgtaat
      361 catattgctc ccagatccag cggcggctgg cgaggtcggg cgaggccatc agcttgagga
      421 ggtccgcggc gatgtcggcg ctctcgggca cgtccgccag cggcgcccgc ttcggcgtcg
      481 gcacccacgg ccggtcgtag catggcgcgt cgtcggcgag cggggcgagg gggatgtcgg
      541 cggcgacttc gccgttgaac ttgagcacca tgtggccggt atcggtgacc tggccgatca
      601 ccgcgaaatc gagctcccac ttgcggaaga tcgcctcggc ctcggcttcg cggccgggct
      661 tcagcaccat cagcatccgc tcctgggatt cggagagcat catctcatag ggcgtcatcc
      721 cctcctcgcg gcaggggacg gcattcatgt cgagctcgag gccgactccg cccttggacg
      781 ccatctcgac cgaggaggag gtgaggccgg ccgcgcccat gtcctggatg gcgacgatcg
      841 cgtcggaggc catcagctcg aggcaggcct cgatgaggag cttctcggtg aacgggtcgc
      901 cgacctgaac ggtcgggcgc ttctcctcgc tgtcctcgga gaaatcggcg gaggccatgg
      961 tggcgccgtg gatcccgtcg cggccggtct tcgagccgac atagacgacc ggattgccga
     1021 tccccgcggc ggccgaatag aagatcttgt cctgctcggc gatgccgacc gtcatcgcgt
     1081 tgacgaggat gttgccgtca taggccgggt gaaagttcac ttcgccgccg acggtcggca
     1141 cgccgacgca attgccgtag ccgccgatgc cggcgacgac gccggcgatc agatgcttca
     1201 tcttcgggtg gtcgggccgg ccgaagcgga gcgcgttcat gttggcgacc ggtcgcgcgc
     1261 ccatggtgaa gacgtcgcgc aggatgccgc cgaccccggt cgcggcaccc tgataaggct
     1321 cgatgtagga agggtggttg tggctctcca tcttgaagat ggcggcctga ttgtcgccga
     1381 tgtcgatcac gccggcattc tcgccggggc cgcagatcac ccagggcgcc gtcgtcggca
     1441 gcttcttcag atggatgcgc gacgatttgt aggagcaatg ctccgaccac atcaccgaaa
     1501 agatgccgag ctcggtgagg ttgggctcgc ggcccagcgc gtggaggacg cggtcatatt
     1561 cctcggggct gaggccgtgc tcggcgacga cgtccgccgt gatgctgctc gtcatggcgc
     1621 gcgccctatc cgatcggaat ccctttgtcg aaggcgcccg tccggtgtac gggtgatcac
     1681 tctttgttct tggtcggtgg aggaaaagac atggcgctga acccgattcc ggaaggctat
     1741 catagcgtta cgccctatct catcgtcgac ggcgcggccg aggcgattcg cttctacggc
     1801 gaggcgttcg gggcggccga ggtgctgcgc atgccgatgg gggacaggat cgcgcatgcc
     1861 gagatcagga tcggcgacag ccacgtcatg ctgtccgacg agatgcccga catgggcttc
     1921 ctcggtccca agacgcgcgg cggaccgacc gcgagcctga tgatctatct cgccgacgtc
     1981 gacgccgcgt tcgagcgggc ggtcgcggcg ggctgcacgg aggagcgccc gcccgcggac
     2041 cagttctggg gcgaccggat gggaacgttg ctcgacccgt tcggccaccg ctggacgctg
     2101 gcgacccatg tggaggacgt ctcggaggag gagatggacc ggcgcatgaa ggaagcgttc
     2161 tgcggggcgg ggtgagggct aaagcccctc cccttgaggg ggaggggttt ggggaggggt
     2221 ggagtctccc gagaaggcgc tgcccatgtg acgagtccac ccccacccaa accctccccc
     2281 ctcaaggggg agggctcagc ggtcgtttcg ccgcaggcgt ttccatgaaa aagccgcccg
     2341 gcgctgtgcc gggcggcctt gttgatccga aactggaacc gctcagccgc gggcgcggca
     2401 gctctgggag cccttgccgg ggacggcgat gttggtgcgc tcggcattgg ccgctaccga
     2461 atggccttcg gcgacataag gcggattgcc gccctcggcg cgcagcatgg tcggcgtgcc
     2521 gccctcggtg gtgcggacct gcgcggtgtt gttggtgtag aaatcgacat agacgaggct
     2581 gttgtcctgg cagcgatagg tccggctcgc ctggatcgag ggcggcagct ccttgggcgc
     2641 cgccttggcg agcgcgtcgc gctggggatc gttgtcggca gggccgaccg tctccggctc
     2701 ggtattgcag gcggaaaggg cgaggctgcc ggcgaccagc gcggcggcaa atgatataag
     2761 gttacgtgcc atggctgcgt ttcttccccc ccaagccctc cgcgtcaagc gcttttgcgg
     2821 ggacggtgtc ccggtaatcc acacaggttc gacaaggccg atccttttcg ttaccccttg
     2881 cgcgtcgttc acgcgggggc ggagagcaat cggcgcatgc agcgcgttcg tatcggtctg
     2941 acgggactcg ccttcgtctt cctcctggtc gtgctcgcga ccatcctcgt gcgtccgagc
     3001 gaggagccgc cgctcagcgc caacaccgtc ggccagccgc gcggtgcgac cggctcgacc
     3061 gccaacagtt cgggcgagcc gaccgagccg ctggcccagc tcggcgtggc tcctggcagc
     3121 gccgagacca acaacagcgc cgctccttcc gccccgccag ttgcccccgc cccggctcgc
     3181 taaaggggcg gcggcgccgc tgatcggggc cggcctgttc ggcgcgtgcg agcttgccgc
     3241 cgggctgctg gtcggccgat tcttcgagtg gggacgggcc gaagcgctgc tgttcctcgc
     3301 cttccggccg tggctgctcg tcgtcggcgc ggcgctggct tcgacctgga ggtggcggca
     3361 gcgggcggtc ttctacaccg ccgccctggt cgccgccggg tcggccgaga cgatcctcct
     3421 cgcctccctc ggcgccgccg acccttggcc ggagctgctc cgcggcctcg ccggcggcgc
     3481 ggcggtggcg ctcgtggccg acatcgcggt gcaaacggcg gggcgctgga ggccgcgcct
     3541 cggcgccttg ttggcggcgg cggtcctgat cgcattgctc gcggccgggg ccatccggcc
     3601 ctacgaggcg ctggtgctcg ggccgaccgt gccgcccgcg cagacggggc ccaagccgcc
     3661 gcttctgctg atgacgagcc tgccgctggt ctggggcccg ggcggtgcct tcgatccgga
     3721 gagtcgcccg tccgagagct atcgcgcgct cgggcgcgag tatgacgtcc gtccgatcga
     3781 tgcgatcgag ccgggctcgc tcgccggcgc ccggctcatg ctcctggccc agccacgcct
     3841 gctcgcgccc gaggagctgg tc
//
LOCUS       DASWNU010000630         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_283890, whole genome shotgun sequence.
ACCESSION   DASWNU010000630 DASWNU010000000
VERSION     DASWNU010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283890"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /gene="tkt"
                     /locus_tag="VGB57_12650"
     CDS             complement(<1..1902)
                     /gene="tkt"
                     /locus_tag="VGB57_12650"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0132240.1"
                     /translation="MAADPRLLANAIRALSMDAVQAANSGHPGMPMGMADVATILFTE
                     YLRHDPADPAWLDRDRFVLSAGHGSMLIYSLLHLTGYSNPTIEEIANFRKLGSPCAGH
                     PENFMLAGVEATTGPLGQGLAMAVGMAIAERHLNAVFGDDLVDHRTWVIAGDGCLMEG
                     VNHEAAGLAGHLGLGRLNVFWDDNRITIDGGTDLSRSEDTIGRYEASGWHTVSCDGHD
                     FADIRRAIDEALADPRPSLIACRTIIGWGAPNKQGTAATHGAALGDEEVAAARKQLVW
                     DSEPFDIPEPILSAWRAAGGRSRQAHEDYRARLAASPDRDEFVRRMEGRLPQGFDISP
                     HLERLMANPQLVATRKASELALEAINAGLPETIGGSADLTGSNNTKTKGQKPLTKEDY
                     SGRYIYYGIREFGMAAAMNGMALHGGLIPYGGTFLVFSDYCRPAIRLSALQGVRVVYV
                     MTHDSIGLGEDGPTHQPVEHLTSLRLIPNLAVYRPADAVETAECWALALERREGPSVL
                     ALTRQNLPQLRLERSENLCARGAYRLRSAGAARRVVLVATGSEVEVALAAADALEGQG
                     IGADVVSMPCWSLFDAQDEDHRRGLLPEDVLKVSIEAGTTLGWERYVGPRGLAIGLDR
                     FGASAPAEDL"
BASE COUNT          339 a          699 c          732 g          323 t
ORIGIN      
        1 caaatcctcc gccggcgccg aagcgccgaa ccggtcgagg ccgatggcga gaccgcgggg
       61 gccgacatag cgttcccagc cgagcgtggt cccggcttcg atcgagacct tgagcacgtc
      121 ctcgggcaga agcccgcgcc ggtgatcctc gtcctgggcg tcgaacagcg accagcaggg
      181 catggacacc acgtccgccc cgatcccctg cccttcgagg gcgtcggccg ccgccagcgc
      241 cacctcgacc tccgagccgg tcgcgaccag gaccacccgg cgcgccgctc ccgcggaacg
      301 cagccggtag gcgcctcgcg cacagaggtt ttcggaccgc tccagcctca gctgggggag
      361 gttctggcgg gtcagggcca gcacggaggg gccctcccga cgctcaagcg ccagcgccca
      421 gcattcggcg gtttcgacag cgtccgcagg acgatagacg gcgaggttcg gaatcagtct
      481 caggctcgtc agatgctcga ccggctggtg ggtggggccg tcctcgccca aaccgatcga
      541 atcgtgggtc atgacgtaga cgacccggac gccctgaagc gccgagagtc ggatggccgg
      601 gcggcagtaa tcggagaaga ccaggaaggt gccgccatag gggatcagtc cgccgtggag
      661 ggccatcccg ttcatcgccg cggccattcc gaactcgcgg atcccgtaat agatgtagcg
      721 gccggaataa tcctctttcg tcagaggctt ctggcccttt gttttggtgt tgttggaacc
      781 ggtcaggtcg gccgagccgc cgatcgtctc gggcaggccg gcgttgatcg cctcgagcgc
      841 cagctccgac gccttgcggg tggcgacaag ttgaggattc gccatcaatc gctccaaatg
      901 tggagagatg tcgaatcctt gcggcagtcg gccttccatc cgccggacga actcgtcgcg
      961 gtccggcgag gccgccaggc gggcccgata atcctcatgc gcctgccgtg aacgcccccc
     1021 cgccgcccgc caggcgctga ggatcggctc gggaatgtcg aacggctcgc tgtcccagac
     1081 cagctgcttc ctcgccgccg cgacctcctc gtcgccgagc gcggcgccgt gggtggcggc
     1141 ggtgccctgc ttgttgggcg cgccccagcc gatgatcgtc cgacaggcga tcagcgaggg
     1201 cctgggatcg gccagagcct cgtcgatggc ccggcggata tcggcgaaat cgtgaccgtc
     1261 gcacgagacg gtgtgccagc cgctcgcttc gtagcggccg atcgtgtcct cggagcggga
     1321 gagatcggtc ccgccgtcga tggtgatccg attgtcgtcc cagaagacgt tgagccgccc
     1381 gagcccgaga tgcccggcaa gcccggccgc ctcgtggttg accccttcca tcaggcagcc
     1441 gtcgccggcg atcacccagg tccggtggtc gaccaggtcg tcgccgaaga cggcgttgag
     1501 atgccgttcc gcgatcgcca tcccgaccgc catggcgagg ccctgaccga gcgggccggt
     1561 ggtcgcctcc acccccgcca gcatgaaatt ctcgggatgc ccggcgcagg ggctgccgag
     1621 cttccggaag ttcgcgatct cctcgatcgt cgggttggaa tagccggtca gatggaggag
     1681 cgagtagatc agcatcgagc cgtggccggc cgaaaggacg aagcggtcgc ggtcgagcca
     1741 ggccggatcg gcgggatcgt gcctgagata ttcggtgaac aggatcgtgg cgacgtcggc
     1801 catgcccatc ggcattccgg ggtggcccga attggcggcc tggacggcgt ccatcgaaag
     1861 cgcgcgaatg gcgttggcga gcagtctcgg atcggcggcc atgcactttc ccaagaggcg
     1921 cccgagcggg ggcgctgagg agagtcggaa cgaccctccg gcaagagcgc tcctttatcg
     1981 gcggcggcgc gcccgtcaac cgcagccggc cctgttttca aaggccgcgg gggcgtgcta
     2041 tgcggcggag catggggcag gacagagtgg aaaaggcgat cggccgcatc gaa
//
LOCUS       DASWNU010000631         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_387049, whole genome shotgun sequence.
ACCESSION   DASWNU010000631 DASWNU010000000
VERSION     DASWNU010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387049"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VGB57_12655"
     CDS             <1..1331
                     /locus_tag="VGB57_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY0132241.1"
                     /translation="KPYEEGVQDERRLFMELMTGTQARAQQYFFFAERKASKIEGLPE
                     DTKARDINRVGVIGAGTMGGGISMNFLSAGIPVTIVEMQQDALDRGTGVMRKNYEASA
                     AKGRITAAQAEGAMGLLKPTLSLDDLADCDLIIEAVFENMDVKKEVFGKLDRIAKPGA
                     ILASNTSYLNIDEIAASTSRPQDVVGLHFFSPANVMKLLEVVRGAKTAPDVLMTAMAL
                     AKKIRKVAVVAGVCYGFIGNRMLMPRQIQSNRLLLEGATPEQIDRVHVEFGMPMGPFQ
                     MADLAGVDIGWHRDPSRIENVRDALCAADRWGQKKGAGFYDYDEKRRPSPSPVVAKII
                     DDFRAKEGVEPREITDQEIVERTLYTMVNEGSKILEEGMAQRASDVDVVWVYGYGWPV
                     WRGGPMFWADSEGLPRIVEGLRRRQERLGPDFSLSELLVRKAEAGEKLTR"
     gene            complement(1343..1507)
                     /locus_tag="VGB57_12660"
     CDS             complement(1343..1507)
                     /locus_tag="VGB57_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0132242.1"
                     /translation="MRYLRHLLRDEDGATAIEYGLIAALIAIAALAAFNRLGLSLVAI
                     FETILAALRG"
     gene            complement(1562..2296)
                     /locus_tag="VGB57_12665"
     CDS             complement(1562..2296)
                     /locus_tag="VGB57_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132243.1"
                     /translation="MPRPNPLTPEVQAAFLAELRSGTLVVAAAARVGVAVQTLYRRRR
                     RDPVFDAGWTAAAEASFGWGWHRLPGGRWRRQWAGAPTARRLRFGAGRRAAYLEALER
                     KGDRSRAARSARVDPRTVRSAFRTDPDFARAHDSALRRGRARRERAAAFERARAAERM
                     IGIVERIAAAMANPVSAFDRHICRLKRRWRPERPGGWRGPGFVRAPAEAIPELRRKIR
                     RIGLAGWKRSRGRGPPWGPGTIPADA"
     gene            complement(2352..2585)
                     /locus_tag="VGB57_12670"
     CDS             complement(2352..2585)
                     /locus_tag="VGB57_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0132244.1"
                     /translation="MDKARAKIVQKAFGARMRSFRKAVKPRLSQEKLGFACGLDRSYI
                     GRIERGESNVGLVNIHRIADALKVEPAEFFREG"
     gene            complement(2671..3087)
                     /locus_tag="VGB57_12675"
     CDS             complement(2671..3087)
                     /locus_tag="VGB57_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132245.1"
                     /translation="MNEEYNRGRRRDLVGYAMDLVDLANGIAELAEREVAPYVGPPIA
                     RQVEELIEARKTRERMFHINLTHPGWTFLLSLYRAHLEGETVELEIGPRHVHLDALCR
                     VAMVKLEDGKARLTDFGARLMEQQFKAEKLALQLLA"
     gene            3195..>3630
                     /locus_tag="VGB57_12680"
     CDS             3195..>3630
                     /locus_tag="VGB57_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEY0132246.1"
                     /translation="MGAVVAKGVFLHKADSVYDDRPEQQYQFPPMYLDRALPLVGDWI
                     VYKGTEKSAHPGYFAVAKVQTIVPDPSAPGRHLALIEPGSYLDFERVVPLRTAEGPVE
                     REAPNLQWAVRPLSIADFNRILALGFPDEEPILPRLGSSIEAP"
BASE COUNT          635 a         1287 c         1122 g          586 t
ORIGIN      
        1 ccaagcccta tgaggaagga gtccaggacg agcgccggct gttcatggaa ttgatgaccg
       61 gaacccaggc gcgggctcag caatatttct tcttcgccga gcgcaaggcc tcgaagatcg
      121 aggggctgcc cgaggatacc aaggcgcgcg acataaaccg ggtgggcgtc atcggcgcgg
      181 gcacgatggg cggcggaatc tcgatgaact tcctgtccgc gggaatcccg gtgaccatcg
      241 tcgagatgca gcaggacgcg ctcgaccgag gcaccggggt gatgcgcaag aactatgagg
      301 cgagcgccgc caagggccgg atcaccgccg cccaggccga aggcgcgatg ggtctcctca
      361 agccgacgct gagcctcgac gacctcgccg attgcgacct catcatcgag gccgtgttcg
      421 agaatatgga cgtcaagaag gaggtgttcg gaaagctcga ccggatcgcc aagcccggcg
      481 ccatcctcgc ctccaatacc tcctatctca acatcgacga gatcgccgcc tcgacctcac
      541 gcccccagga cgtcgtcggc ctccacttct tctcccccgc caacgtcatg aaattgcttg
      601 aggtcgtgcg cggagcgaag accgcgcccg acgtcctgat gaccgcgatg gccctcgcca
      661 agaagatccg caaggtcgcg gtggtggcgg gcgtctgcta cggcttcatc ggcaatagga
      721 tgctgatgcc gcgccagatc cagtccaacc gcctcctcct cgaaggcgcc acccccgagc
      781 agatcgaccg ggtccatgtc gagttcggga tgccgatggg gcccttccag atggccgatc
      841 tggccggggt cgacatcggc tggcaccgcg acccgtcccg gatcgagaac gtccgcgacg
      901 cgctctgcgc cgccgaccgc tggggccaga agaagggcgc cggcttctac gattacgacg
      961 agaagcgccg cccctccccg tcaccggtgg tcgcgaagat catcgacgat ttccgagcca
     1021 aggaaggcgt cgagccccgc gagatcaccg accaggagat cgtcgagcgc accctctaca
     1081 cgatggtcaa cgagggctcg aagatcctcg aggaaggcat ggcccagcgc gcctccgacg
     1141 tggacgtcgt ctgggtctac ggctacggct ggccggtctg gcgcggcggc ccgatgttct
     1201 gggccgactc ggaagggctg cccaggatcg tcgagggcct gaggcgccgg caggaaaggc
     1261 tgggcccgga tttctcgctg tcggagctgc tggtgcgcaa ggcggaggcc ggcgagaagc
     1321 tgacccgctg agtttaccgt cgttaccccc ggagggccgc caggatcgtc tcgaagatcg
     1381 cgaccaggct taggccgagg cggttgaagg cggcgagcgc ggcgatcgcg atcagagccg
     1441 cgatgagccc atattcgatc gccgtcgccc cgtcctcgtc gcggagcaaa tgtctgagat
     1501 agcgcaactt cataccccct tgccaagtgc cccttcatgg ccggcgaagg ttatttcttg
     1561 cttaagcatc ggctggaatc gtgccggggc cccacggagg cccccgcccg cgcgagcgct
     1621 tccacccggc gagcccgatc cgccggatct tgcgcctgag ctccgggatc gcctccgccg
     1681 gagcccggac gaagccgggg ccccgccagc cgcccggccg ctcgggccgc cagcggcgct
     1741 tgagccggca gatgtgccgg tcgaaggcgc tcaccggatt cgccatcgcc gccgcgatcc
     1801 gctccacgat cccgatcatc cgctccgccg cccgcgcccg ctcgaaagcc gccgcccgct
     1861 cccgccgggc gcggccgcgc cgcagcgccg agtcatgggc gcgcgcgaag tccggatcgg
     1921 tccggaaggc gcttctgacc gtcctcggat cgacccgcgc cgaccgcgcc gcccggctgc
     1981 gatcgccctt ccgctcgagc gcttcgagat aggccgcccg ccgccccgcc ccgaagcgca
     2041 gccgccgtgc cgtcggcgcc cccgcccact gccggcgcca gcgcccgccg ggaagcctgt
     2101 gccagcccca cccgaagctc gcctccgccg ctgccgtcca cccggcatcg aagaccgggt
     2161 cgcgcctgcg ccgccggtaa agcgtctgca ccgccacccc caccctggcc gccgccgcca
     2221 ccaccagcgt cccgctgcga agctcggcaa ggaaggcggc ctggacttcg ggcgtcagcg
     2281 ggttgggcct gggcatatgc ctcaaggtac cacataaggc cccgagaaga aagaacagaa
     2341 taagaacgag gtcagccttc ccggaagaac tccgccggct caaccttcag agcgtcggcg
     2401 atgcggtgga tgttgactaa ccccacattg ctttcccccc gctcgatccg gccgatgtag
     2461 gaacggtcga gcccacaggc gaagccgagc ttttcctggg acagcctcgg cttcacggcc
     2521 ttccgaaagc ttcgcatgcg ggccccaaag gccttttgca caattttcgc ccgcgccttg
     2581 tccatgcccg ggacttggac aaaatgcgga ctttaattcc acggacgatt agtcgcattc
     2641 ggtgtccaaa atggtgcatc cgaaaatcgt ttaggcgagc agctggagcg cgagcttctc
     2701 cgccttgaac tgctgctcca tcagccgggc gccgaaatcg gtgagtcgag ctttcccgtc
     2761 ctccagcttg accatcgcca cccggcacaa cgcgtcgagg tgcacgtgcc tcggtccgat
     2821 ctcaagctcc accgtctccc cctccaggtg cgcccggtac agcgacagaa ggaacgtcca
     2881 gcccggatgc gtgagattga tatggaacat gcgctcgcgg gttttccgag cttcgatcag
     2941 ctcctccacc tgccgagcga tcggcggccc cacatagggc gccacctccc gctcggccag
     3001 ttcggcgatg ccattggcga ggtccaccag atccatcgca tatccgacca gatcccgccg
     3061 tcggccgcgg ttatattctt cattcatttg cggactataa tacccgtctc gtcggacccc
     3121 aaattcggat ggagcaagga cgcaggtggc ttgccgtcct ctcggcacct gctagcctga
     3181 cattcgaaaa gctgatgggg gcggtggtgg cgaaaggggt gtttctgcac aaggcggact
     3241 cggtctacga cgaccgcccg gagcagcaat atcaatttcc cccgatgtat ctcgaccggg
     3301 cgctcccctt ggtgggcgat tggatcgtct acaaaggcac ggagaagagc gcccaccccg
     3361 gctatttcgc cgttgccaag gtccagacca tcgtccccga cccgtcggct cccggtcgcc
     3421 acctcgctct gatcgagcct ggctcctatc tcgatttcga acgggtggtt ccacttcgca
     3481 ctgccgaagg accggtggag cgcgaagccc cgaacctgca gtgggccgtg cgcccgctct
     3541 ccatcgcgga cttcaaccgg atcctggctc taggcttccc ggacgaagag cccatcctcc
     3601 cccgtctggg cagctcgatc gaagcgcctt
//
LOCUS       DASWNU010000632        11992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_517092, whole genome shotgun sequence.
ACCESSION   DASWNU010000632 DASWNU010000000
VERSION     DASWNU010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11992
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517092"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /gene="pgi"
                     /locus_tag="VGB57_12685"
     CDS             <1..1394
                     /gene="pgi"
                     /locus_tag="VGB57_12685"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0132247.1"
                     /translation="AKTHLDAELLARFTALAERTDLAAKRDALFAGAPVNATEGRAAE
                     HTAERGEGAPDSVELAARFHARTRTLIDAVEADAFGPVRHILHIGIGGSALGPDLLID
                     ALGRDAGRYETAIVSNVDGAALAEAIRSFDPASTLLVVASKTFTTSETMLNTRSALQW
                     MEENGVGDPYGRVIALTASPDRALEFGVDETRILPFAESVGGRYSLWSSIGFPAALAL
                     GWEAFEELLEGAAAMDRHFRLQPLARNAPVLAAFVDRYYANVRGSQTRAVFAYDERLR
                     LLPSYLQQLEMESNGKSVKLDGSPVGRETSLITWGGVGTDAQHAVFQLLHQGTHLVPV
                     EFVACVEPDHGFDDEHHRQLLVNCFAQGAALMSGKGNDDPHRNYPGDRPSTTILLDRL
                     DPATLGALIAFYEHRTFANAVLLGINPFDQFGVELGKSMAKAIDGGEEIEFDPSTKAL
                     IERALPDPSLRGA"
     gene            1469..2098
                     /locus_tag="VGB57_12690"
     CDS             1469..2098
                     /locus_tag="VGB57_12690"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0132248.1"
                     /translation="MLILIYLYDGVTALDAIGPYESLSRLPGVEIRFVGRRAGPVRTG
                     DGFLGLHADTGINDEIQADMLIVPGGRRAGVQSEIGEERVLGWMAVLDAVTTRTCSVC
                     TGSLILGAAGLLEGRRASTHWGARDYLGHFNATYSAERVTEDGKYMTSAGVSAGIDLG
                     LRLCAEIAGREMAEAVQLSMEYDPDPPFNAGNFRNATPERLANVAKLNI"
     gene            2264..3607
                     /gene="gorA"
                     /locus_tag="VGB57_12695"
     CDS             2264..3607
                     /gene="gorA"
                     /locus_tag="VGB57_12695"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY0132249.1"
                     /translation="MPQSYDYDLFVIGAGSGGVRAARVSSAHGAKVAIAEEHKVGGTC
                     VIRGCVPKKLLVYGAHFAEDLQDAPRFGWDVPACNFHWPTLRDNVLAEVGRLEEAYTD
                     TLTNHKVEILKERAVVAGPHEVRVGGRTVTAGIILIATGARPVVPDFPGSELGITSNE
                     AFHLPDLPRRVVIAGAGYIANEFAGIFHNLGSHVTLVNRTDVILRGYDETIRDRLLTI
                     SMMKGIDFKFHVSFESIEKQEGALRVNMSGCDSIEADAVMFAIGRQPNTEGLGLESAG
                     VKLDDKGAVVVDADNRSSCDSVYAVGDVTNRVQLTPVAIREGQAFADSIFGNKPSRVD
                     YENIPSAVFSHPPLAGVGMTEGEARNRLGSVKTYTSDFRAMKNVLAGRNERALYKLVV
                     NSETDEVVGLHMIGPDAPEILQAAAIAVKAKLKKQDFDAVVALHPTMAEELVLMR"
     gene            complement(3750..5078)
                     /locus_tag="VGB57_12700"
     CDS             complement(3750..5078)
                     /locus_tag="VGB57_12700"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0132250.1"
                     /translation="MRILKQSMAAALALVAAPLAAQQAAAPAASVPAAGLDNRAVVAE
                     IRRVVAERYVLPERRPALDAALAEALAAGRYDVAEPNLLAERINEDLARVGRDRHLNF
                     SFDPRRAAILAARSNQKAPDPSGFERQVREANHGITELRLLPGNVRYMAYDGFLWIGP
                     ESAAALDTAIRFLGGGQAIIIDLRRNGGGDADASQYLISHFLPADQVLYTYYEKAGAQ
                     ATRVSTLPAVAGGRLIGKPLYVLTSGATASAAEEFAGNVGGFKLGELVGEKTAGAGYP
                     NDLVPIDGRFVLSVSTGRVVLASTARDWEAVGIAPTIPTAAGQALDAAQVHALRLLLA
                     VMPEAERPRMEALAEGISARLEVRAPALALAAYAGSFGERRLVAEAGNLYYQRGDRPR
                     SLLIPLGGNRFAFDNDPALQLEFGAGDKAAKAFTILRPGLPPQGTYERTE"
     gene            5317..6609
                     /gene="hmgA"
                     /locus_tag="VGB57_12705"
     CDS             5317..6609
                     /gene="hmgA"
                     /locus_tag="VGB57_12705"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY0132251.1"
                     /translation="MTDPAYLSGFGNHFATEAVPGALPEGRNSPQKPPFGLYAEQLSG
                     TAFTAPRHENRRSWLYRLRPSAEHPPYRSYDAAPRFTASGLQTPLPPNRLRWNPLELG
                     EDRADLIDGMTTMMANGGPEALSGVAVHIYRAARDMERCFFNADGEMLFLPEHGRLAL
                     LTEMGRIDLAPGEVGLVPRGVRFRALLPDGAARGYVAENHGALFRLPELGPIGSNGLA
                     NARDFLTSVAAYEDVDGEVELIQKYLGRLWTTTLDHSPLDVVAWHGNLAPWKYDVARF
                     NTIGTVSFDHPDPSIFTVLTSPSETPGRANADFVIFPPRWMVAEDTFRPPWFHRNVMS
                     ECMGLIKGAYDAKAEGFAPGGLSLHNLMSGHGPDVASWKAASEAELRPSKIEDTLAFM
                     VESCWPFRPTAFAMSGEGGLQPDYDRAWGGFPKARPEG"
     gene            6613..7266
                     /locus_tag="VGB57_12710"
     CDS             6613..7266
                     /locus_tag="VGB57_12710"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0132252.1"
                     /translation="MATLPPRPFPPCHRYRDFLDPGEHEALLEWAIASRGRYEPARLT
                     GHVVDPSRRIAERLNDLGPHRPVLERRLKEHLPDIFRRTGTRPFEIEHFELEVAAHGD
                     GAFFAHHSDMPVGRGRKPLGGDKTGKQDRLVSCVYYFHREPKRFSGGTLRLYRLGDHD
                     ASGDFVEFEPEQNSLVVFPSWVRHEVRRISCPDCSFEDYRFAVNAWLCKTLAPGPAS"
     gene            complement(7397..8719)
                     /locus_tag="VGB57_12715"
     CDS             complement(7397..8719)
                     /locus_tag="VGB57_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0132253.1"
                     /translation="MRISMLVLVAGLAMLPAAARAQAPAAPERPAPADGRTVVAEIRR
                     LLAANYVLPEVRPRLDAILAKGLAEGRYDVSDPAALLDRINSDLTSVARDKHLSLEYD
                     PRQAAEIAAEPADQPEDSGPSAEQVRQAQRRNHGFTEMKVLPGNVRYVNLQGFVWAGP
                     KSAEAYDNAMRFLKEGDAAIIDLRQNGGGSPQAVQYLVSHFMEPNRPLVTFHMGANQV
                     DRLSTLPTLPAGRMVGKPLYVLTSGRSASAAEEFTGHVAGFRLGELIGATTAGAGFRN
                     QFFPLPGGYLISISVGRAVLASTGKDWEGVGIAPTTAVDPEKALQVAQVHAMRRLASA
                     APPREKANYEATAALLAAQVDPVATALPLTAYAGNFGERKVSLENGRLAFQRQGGPKL
                     AMVPIGPNLFAFEDDAMTKVEYKVAGNAATGFELQRGDGSKVEAQRTP"
     gene            8843..9322
                     /locus_tag="VGB57_12720"
     CDS             8843..9322
                     /locus_tag="VGB57_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0132254.1"
                     /translation="MDKKLDPLDLKLLTWVQVDNLLTTEMLAERVGLSPSAVARRLRH
                     LRTTRAIAGDVAIVSERALGRPLSAIIHIQLERHALTEVASFLRLLAASENIQFCLEI
                     SGAFDVLLLVVVADMEAFNLFADDMLAGQRAVRRYETSFVKRRLKASLALPLEQLAG"
     gene            9454..11034
                     /locus_tag="VGB57_12725"
     CDS             9454..11034
                     /locus_tag="VGB57_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0132255.1"
                     /translation="MTTDVLIIGAGHNGLVCAYYLAAKGLKVTILERRHVVGGAAVTE
                     EFHPGFRNSTAAYTVSLLNPKVIEDMRLYEHGLKVVLRKIDNFLPTLGPEHLLSGRDG
                     LTRREIERHSLKDAEGYDAYSAALGSVVAILRDWILRAPPNAGGGLADVLAALKLGNA
                     VRGMSAEEQRNLLDFFTKAAADILERYFSTDIVQALFGFDAVVGNYASPYDPGSAYVL
                     LHHLFGEAAGVAGAWGHAIGGMGAITQAMARAARSKGVEIVLDSPVDEIIVERGRAAG
                     AVAGGKSYRAKAVAANVNPRFLFGSLLPDGAVSPEISLRMAGWAAESATFRMNLALSE
                     LPRFTSLPQAGDHLTAGIIVAPSLGYMDRAYTSARTEGWAREPIVEMLIPSTLDDSLA
                     PPGSHVASLFCQHFPYRVDGGWEARVDEAADHIVAHVDRFAPGFKASVIGRLALSPWH
                     LEQRFGIPRGDIFHGKMGLDQLFSARPMLGHADYRMPLAGLYLCGSGAHPGGGVTGAP
                     GHNAAQAILADRRKWRRA"
     gene            complement(11122..11754)
                     /locus_tag="VGB57_12730"
     CDS             complement(11122..11754)
                     /locus_tag="VGB57_12730"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY0132256.1"
                     /translation="MKVVLAAALPLALAACDMIAPPPPEAAKVDYRPEEAGTVDHALC
                     LLGFTAIPLREARGTGHHLVTAAVNGKEGVFILDTGANLSVIDVDHVAHFGLAAARGR
                     PGGATGLGGSNVARQVSIDSLAFGAVKLRQRRMVVTDLGSIGDALAPLAPGGAVHGII
                     GQDVLKEHRAVIDVERPLLYLIEADEDPEPVPAERCRAKAEPAERSTKRD"
     gene            11804..>11992
                     /locus_tag="VGB57_12735"
     CDS             11804..>11992
                     /locus_tag="VGB57_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0132257.1"
                     /translation="MKTRAAIAVAPNRPLEIHEVDLEGPRAGEVLVEIMATGVCHTDA
                     YTLEGKDSEGIFPSILGHE"
BASE COUNT         1887 a         4304 c         3913 g         1888 t
ORIGIN      
        1 tggccaagac gcacctcgat gccgagctac tggcgcgctt caccgctttg gccgaacgca
       61 ccgacctggc cgcaaagcgc gacgcgctct tcgccggcgc gcccgtcaat gccaccgagg
      121 gccgcgccgc cgaacatacg gcggaacgcg gggagggggc tcccgacagc gtcgaactgg
      181 ccgcccgctt ccacgcccgc acccgcaccc tgatcgacgc ggtcgaggcc gacgcgttcg
      241 ggccggtgcg ccacatcctc catatcggca tcggcggctc ggcgctgggc ccagacctgc
      301 tgatcgacgc cctgggccgc gacgccggcc gctacgagac cgcgatcgtc tccaacgtcg
      361 acggcgcggc actcgccgag gcgatcagga gcttcgatcc cgcgtcgacg ctactggtgg
      421 tcgcctccaa gaccttcacc accagcgaga ccatgctcaa cacccgctcc gcactgcagt
      481 ggatggagga aaatggcgtc ggcgacccct acggccgggt catcgcgctg acggcgagcc
      541 ctgaccgagc gctcgaattc ggagtggacg agacccgcat cctgccgttc gccgagagcg
      601 tcggcggccg ctattcgctc tggtcctcca tcggctttcc ggccgccctc gcgctcggct
      661 gggaggcgtt cgaggagctg ctcgaaggcg ccgccgcgat ggaccggcat ttccgcctcc
      721 agccgctggc ccggaacgcg cccgtcctcg ccgccttcgt cgaccgctat tacgcgaacg
      781 tccgaggcag ccagacccgg gccgtcttcg cctatgacga gcggctccgg ctcctgccct
      841 cctatctcca gcagctcgaa atggagtcga acggcaagtc ggtgaagctc gacggttctc
      901 cggtgggacg cgagacctcg ctgatcacct ggggcggagt cggcaccgat gcccagcacg
      961 ccgtcttcca gctgctccac cagggcaccc atctggtgcc cgtcgagttc gtcgcctgcg
     1021 tcgagcccga ccacggcttc gacgacgagc atcaccggca gctcctcgtc aattgcttcg
     1081 cccagggcgc ggcgctgatg tccggcaagg ggaatgacga cccgcatcgg aattatcccg
     1141 gcgaccgccc ctcgacgacg atcctcctcg accggctcga tccggccacg ctcggagctc
     1201 tgatcgcctt ttacgagcac cgcaccttcg cgaacgcggt gcttctcggg atcaatccgt
     1261 tcgaccagtt cggagtggag ctgggcaagt cgatggcgaa ggctatcgac ggcggcgagg
     1321 agatcgagtt cgacccgtcg accaaggcgc tgatcgagcg ggccctgcct gatccgtcat
     1381 tgcgaggagc gtagcgacga agcaatccag cgggcggcgg cgcggcactg gattgcttcg
     1441 ctgcgctcgc aatgacgaag gaacgaccat gctgatcctc atctacctct acgacggagt
     1501 caccgccctc gacgcgatcg gcccctatga atccctgagc cggctgccgg gcgtggagat
     1561 ccgcttcgtc ggccgccgcg ccgggcccgt gcgcaccggc gacggcttcc tgggcctcca
     1621 tgcggacacc ggcataaacg acgagatcca ggccgacatg ctgatcgtcc ccggcggcag
     1681 gcgcgccggc gttcagagcg agatcggcga ggaaagggtg ctgggctgga tggcggtgct
     1741 cgatgccgtc acgacccgga cctgctcggt ctgcaccggc tcgctcatcc tcggcgcggc
     1801 cggactgctg gagggacgcc gtgcctcgac ccattggggc gcccgcgact atctcggcca
     1861 tttcaacgcc acctattccg ccgagcgggt caccgaggac ggcaagtaca tgaccagcgc
     1921 cggagtctcg gcgggcatcg acctcggcct tcgcctgtgc gccgagatcg ccggccggga
     1981 gatggcggaa gcggtccagc tcagcatgga atacgacccc gatccgccgt tcaatgccgg
     2041 caatttcagg aatgccacgc cggagcggct ggcgaacgtg gcgaagctga acatctagcc
     2101 gtcattgcga gcgtagcgaa gcaatccagc gccgagtccg tcgcggacgc tgcggctgga
     2161 ttgccgcgtc gcttcgctcc tcgcaatgac ggaggcggcg ccgtttgcat gggcctgaca
     2221 tcgcctccat atgccctccg ttcctcctga cgaaagcgcc cccatgcccc agagctacga
     2281 ctacgacctc ttcgtcatcg gcgccggatc gggcggcgtg cgcgccgctc gggtctcctc
     2341 ggcgcatggc gcgaaggtgg cgatcgccga ggagcataag gtgggcggca cctgcgtcat
     2401 ccgcggctgc gttcccaaga agctgctcgt ctacggcgcc catttcgccg aggatctgca
     2461 ggacgcgccc cgcttcggct gggacgtgcc ggcctgcaac ttccactggc cgacccttcg
     2521 cgacaacgtc ctcgccgagg ttggccggct cgaggaggcc tataccgaca ccctgaccaa
     2581 ccataaggtc gagatattga aggagcgcgc ggtcgtcgcc ggcccccacg aggtccgggt
     2641 cggcggccga accgtcaccg ccggaatcat tctcatcgcc accggcgccc gcccggtcgt
     2701 gcccgatttc cccggcagcg agctcgggat cacttcgaac gaggccttcc accttcccga
     2761 tctgccaagg agggtggtga tcgccggcgc cggctacatc gccaacgagt tcgccggaat
     2821 cttccacaat ctgggaagcc acgtgaccct ggtgaaccgc accgacgtca tccttcgcgg
     2881 ctatgacgaa acgatccgcg accgtcttct caccatctcg atgatgaagg ggatcgactt
     2941 caagttccac gtctcgttcg agagcatcga gaagcaggaa ggggcgctca gggtgaacat
     3001 gtccggctgc gactcgatcg aggcggacgc ggtgatgttc gcgatcggcc gccagcccaa
     3061 taccgagggt ctcggcctcg aaagcgccgg agtgaagctc gacgacaagg gggcggtggt
     3121 ggtcgacgcc gacaacagat cgagctgcga cagcgtctac gcggtcggcg acgtcaccaa
     3181 ccgggtccag ctcacgcccg tcgcgatccg cgagggccag gccttcgccg attccatctt
     3241 cggcaacaag ccgagccggg tcgactatga gaatatcccg tcggccgtgt tcagccaccc
     3301 gccgctcgcc ggggtcggga tgaccgaggg cgaggcgcgc aacaggctcg gctcggtcaa
     3361 gacctacacg tccgacttcc gggcgatgaa gaacgtgctc gccggccgca acgagcgggc
     3421 gctttacaag ctcgtcgtca actcggagac ggacgaggtg gttgggctcc acatgatcgg
     3481 ccccgacgcc cccgaaatcc tccaggccgc cgccatcgcg gtcaaggcga agctcaagaa
     3541 gcaggatttc gacgccgtcg tggcgcttca cccgaccatg gccgaagagc tggtgctgat
     3601 gcgctgaggc ctggaacggc gtgcgaaacc tgaatcgtca ccccgggctt gacccggggt
     3661 ccacctactt cctgggtcat gccgaaaaga cggtggatgc cgggtcaagc ccggcatgac
     3721 gagatggggc agatccagcg cacgccgctc tactcggtcc gctcgtacgt gccctggggc
     3781 ggcaggccgg ggcgcaggat cgtgaacgct ttcgccgcct tgtcgccggc gccgaattcc
     3841 agctgcaggg cgggatcgtt gtcgaaggcg aaacggttgc cgccgagcgg gatcagaagc
     3901 gaccgggggc ggtcgccgcg ctgataatag aggttgccgg cttccgcgac cagccgcctc
     3961 tcgccgaacg aaccggcata agcggcgagc gccagcgccg gggcgcgaac ctccaagcgg
     4021 gcggagatgc cctccgccag ggcttccatc cgcggccgtt ccgcctccgg cattacggcg
     4081 aggagcaagc gaagcgcgtg gacctgcgcc gcgtccagcg cctgcccggc ggccgtcggg
     4141 atcgtcggcg cgattccgac cgcctcccag tcccgggcgg tcgaggcgag caccacccgg
     4201 ccggtcgaaa cgctcagcac gaaacgcccg tcgatcggca ccagatcgtt tggatagccg
     4261 gccccggcgg tcttctcgcc gaccagctcg cccagcttga atcctcccac attgccggca
     4321 aactcctcgg ccgcggaggc ggtggcgccg ctggtaagga cataaagcgg cttgccgata
     4381 agacgccccc ccgcaaccgc cggaagtgtc gacacgcggg tcgcctgggc cccggccttc
     4441 tcataataag tgtagagcac ctggtccgcc ggcaggaaat ggctgatcag atattgggac
     4501 gcgtccgcat cccctccgcc gttgcggcga aggtcgatga tgatggcctg accgccgccg
     4561 aggaagcgga tggcggtgtc cagcgctgcg gcgctctccg ggccgatcca caggaacccg
     4621 tcataggcca tgtagcggac gttgccgggc agcagccgaa gctcggtgat tccgtgattg
     4681 gcttcgcgga cctgccgttc gaagccgctc ggatccggcg ctttctggtt gctcctggcg
     4741 gcgaggatcg ccgcgcgccg cgggtcgaag ctgaaattga ggtgccggtc gcggccgacg
     4801 cgcgcgagat cctcgttgat ccgctcggcc agcaggttcg gctcggcgac gtcgtagcgt
     4861 ccggccgcca acgcttccgc gagggccgcg tcgagggccg gacgccgctc cggaaggacg
     4921 tagcgttcgg cgacgacccg acggatttcc gccacgacgg cgcggttgtc gagcccggcg
     4981 gcggggacgg aagcggccgg cgccgccgcc tgctgtgccg ccagaggcgc cgcgaccagc
     5041 gccagggcgg ccgccatgga ctgcttcaaa atccgcatgg tccggctccc gatcgatcga
     5101 ggcaagtatc gcccgggatc ggtgccgacc tcgcccgaat agatgggccg ggttgccaga
     5161 atctggcagc tccagggcgt gaaaggcgta aagcgggcaa ggacgtcggc aagctcgact
     5221 ccccgtcacc cggcggaagc cgggaccccg tgacgggagg gcgcaggcgc gctatcgacc
     5281 tgaggccccg gcttccgccg gggtgacgag gaatcgatga ccgaccccgc ctacctctcc
     5341 ggcttcggca accatttcgc caccgaggcc gtgcccggcg cccttcccga gggccgcaac
     5401 tcgccccaga agcccccctt cggtctctat gccgagcagc tctcgggcac cgccttcacc
     5461 gcgcctcgcc acgagaaccg ccgctcctgg ctctaccgcc ttcgcccgag cgccgagcac
     5521 ccgccctacc ggtcctatga cgccgcgccg cgcttcaccg cctccggcct ccagaccccc
     5581 ctccccccca accggcttcg ctggaacccg ctggagcttg gggaggacag ggccgacctg
     5641 atcgacggaa tgacgacgat gatggccaat ggcggcccgg aagcgctgtc cggagtcgcc
     5701 gtccatatct accgcgcggc ccgcgatatg gagcgctgct tcttcaacgc cgacggcgag
     5761 atgctcttcc tccccgagca cggccgcctc gccctgctca ccgagatggg ccggatcgac
     5821 ctggcgcccg gcgaagtcgg gctggtgccg cgcggcgtgc gcttccgcgc cctcctcccc
     5881 gacggagccg cgcgcggcta tgtcgccgag aatcacggcg ccctcttccg gcttcccgag
     5941 ctcggcccga tcggctccaa cggcctcgcc aacgcccgcg acttcctcac gtctgtggcg
     6001 gcctatgagg acgtcgacgg cgaggtcgag ctcatccaga aatatctcgg ccggctgtgg
     6061 acgacgacgc tcgaccacag cccgctcgac gtcgtcgcct ggcacggcaa tctcgccccc
     6121 tggaaatacg atgttgcccg gttcaacacg atcggcaccg tcagcttcga ccatcccgac
     6181 ccgtccatct tcaccgtcct caccagcccg tccgagactc cgggccgcgc caatgccgat
     6241 ttcgtcatct tcccgccgcg gtggatggtg gccgaggaca cgttccggcc gccctggttc
     6301 caccgcaacg tgatgagcga gtgcatgggg ctgatcaaag gcgcctacga cgccaaggcg
     6361 gaaggcttcg cgcccggcgg cctcagcctc cacaacctca tgtccgggca cgggcccgac
     6421 gtggcgagct ggaaggccgc ttccgaggcc gagctcaggc ccagcaagat cgaggacact
     6481 ttggccttca tggtcgaaag ctgctggccc ttccgcccca ccgcgttcgc catgtcgggc
     6541 gagggcggcc tgcagcccga ttatgaccgg gcctggggcg gcttccccaa ggcgcggccc
     6601 gagggctgag agatggcgac cctgcccccg cggccgttcc cgccctgcca ccgatatcgc
     6661 gacttcctcg atcccggcga gcatgaggcc ctgctcgaat gggcgatcgc cagccgcggc
     6721 cggtacgagc ccgcccggct taccggccat gtcgtcgacc ccagccgccg gatcgccgag
     6781 cgcctcaacg acctcggccc gcaccggccg gtgctcgaac ggcggctgaa ggagcatctg
     6841 ccggacatct tccgccgcac cggcaccagg cctttcgaga tcgaacattt cgagctcgag
     6901 gtcgccgccc acggcgacgg cgccttcttc gcccatcaca gcgacatgcc cgtcggccgc
     6961 ggccgcaagc cgctcggcgg ggacaagacg ggcaagcagg accgccttgt cagctgcgtc
     7021 tattatttcc accgcgagcc caagcgcttc tcgggcggga ccctgcgcct ctatcggctc
     7081 ggcgaccacg atgcgtcggg cgacttcgtc gagttcgagc ccgagcagaa cagcctcgtc
     7141 gtcttcccct cctgggtccg ccacgaagtg cgccggataa gctgccccga ttgcagcttc
     7201 gaggattatc gcttcgcggt gaacgcctgg ctctgcaaga ccctggcgcc ggggccggcc
     7261 tcttaattcc tccccgcgat ttcgcgggga gggggaccag gcgaagcctg gtggaggggt
     7321 tcttcaggct ccgaagatcc cctccaccgc gctacgcgcg gtccccctcc ccgggcaaat
     7381 gccggggagg aattccctac ggcgtccgct gcgcctccac cttgctgccg tcgccgcgct
     7441 gcagctcgaa gccggtggcg gcgttgcccg ccaccttata ttcgaccttc gtcatcgcgt
     7501 cgtcctcgaa cgcgaacagg ttcggcccga tcgggaccat cgccagcttc gggccaccct
     7561 gccgctggaa ggcgaggcgt ccgttctcga gcgacacctt ccgctccccg aaattgccgg
     7621 cataggcagt gagcggcaaa gcggtggcga cgggatcgac ctgggcggcg agcaaggcgg
     7681 cggtcgcctc gtaattcgcc ttctcccgcg gcggcgcggc cgaggcgagg cggcgcatgg
     7741 catggacctg ggcgacctgg agcgcctttt ccggatcgac ggcggtggtc ggggcgattc
     7801 cgacgccttc ccaatccttc ccggtggagg cgagcacggc ccgccccacc gagatgctga
     7861 tcagatagcc gcccggcaag ggaaagaact ggttgcggaa gcccgcgccc gcggtcgtcg
     7921 ctccgatcag ctcgcccagc ctgaagccgg cgacatggcc ggtgaactcc tcggccgcgc
     7981 tcgcgcttcg gccgctggtc aggacgtaga gcggcttgcc gaccattcgc ccggcgggca
     8041 gagtcggcag ggtcgacagg cgatccacct ggttcgcgcc catgtggaag gtcaccagcg
     8101 gtcggttcgg ctccatgaaa tggctgacca gatattggac cgcctgcggg cttccgccgc
     8161 cattctggcg aaggtcgatg atcgccgcgt cgccttcctt gaggaagcgc atcgcattgt
     8221 cataggcctc ggcgctcttc ggtccggccc agacgaagcc ctgcaggttc acgtagcgga
     8281 cgttgccggg aagcaccttc atctcggtga agccgtggtt gcgccgctgc gcctggcgga
     8341 cctgctcggc cgaagggccg gaatcttccg gctggtcggc gggctcggcg gcgatctcgg
     8401 ccgcctggcg cggatcatat tcgaggctga gatgcttgtc gcgggcgacg gaggtgagat
     8461 cggaattgat ccgatcgagc agcgccgccg gatcggagac gtcgtagcgg ccctccgcca
     8521 gccccttggc gaggatcgcg tcgagcctgg ggcggacctc cggaaggacg taattggcgg
     8581 cgagcagccg ccttatctcg gcgaccaccg tccgcccgtc ggcgggcgcg ggccgctccg
     8641 gcgcggccgg agcctgggcc cgggcagcgg ccggaagcat cgccagtccg gcgacgagga
     8701 ccaacatgct gatacgcatt gaagctctcc tatggattcc acgtcatccc acacctgtta
     8761 tcgcccgagc tatcgccagt ttctggcaaa gatcgaacgt atttcagctg ttattggcga
     8821 aagaggcggt taagctgccg aagtggacaa gaagctcgac ccgctcgacc tcaagctgct
     8881 cacctgggtg caggtcgaca atctgctgac cacggaaatg ctggccgagc gggtcggcct
     8941 ttccccctcc gccgtggcgc gccgcttgcg gcacctgcgg acgacccggg cgatcgccgg
     9001 cgacgtcgcc atcgtctccg agcgggccct cggccgcccc ctttcggcca tcatccatat
     9061 ccagctcgag cgtcacgccc tgaccgaggt ggcgagcttt ctgcggcttc tcgccgcgtc
     9121 ggagaacatc cagttctgcc tcgagatttc aggcgcgttc gacgtcctcc tgctcgtcgt
     9181 cgtcgccgac atggaggcgt tcaacctctt cgccgacgac atgctcgccg gccagcgcgc
     9241 ggtgcgccgc tacgagacca gcttcgtcaa gcgccggctc aaggcgagcc tggcgctgcc
     9301 gcttgagcag ctggcggggt gaggccgtcg ccccggcgaa ggctggggcc cggggggtga
     9361 aaaacaccgg tgcctttcac ccctggggtc ccgcccttcg ctgggacgac gctccgattt
     9421 gctacgtcat ggccttgcct atagtcggcc gtgatgacca ccgacgtcct catcatcggc
     9481 gccggccata acggcctcgt ctgcgcttat tatctcgccg cgaaggggct gaaggtcacc
     9541 atcctcgagc gccgccacgt cgtcggcgga gcggcggtga cggaggagtt ccaccccggc
     9601 ttccgcaact cgaccgcggc ctacacggtc agcctgctca acccgaaggt gatcgaggac
     9661 atgcggctct acgagcacgg cctcaaggtc gtgcttcgca agatcgacaa tttcctgccg
     9721 acgctgggcc cggagcacct cctttccggc cgcgacggcc tgacccggcg ggagatcgag
     9781 cgccactccc tgaaggacgc cgaaggctat gacgcttatt cggccgccct gggctcggtc
     9841 gtcgccatcc ttcgagactg gatattgcgc gccccgccca atgccggagg cggcctcgcg
     9901 gacgtcctgg ccgccctgaa gctcggcaat gccgttcgcg gcatgagcgc ggaggagcag
     9961 cgcaacctgc tcgatttctt caccaaggcc gccgccgaca tcctcgaacg ctatttcagc
    10021 accgacatcg tgcaggccct gttcggcttc gacgccgtcg tcggcaatta tgccagcccc
    10081 tacgatcccg gctcagccta cgtcctcctc caccatctgt tcggcgaggc ggccggggtg
    10141 gccggcgcct ggggccatgc gatcggcgga atgggcgcga tcacccaggc catggccagg
    10201 gccgcgcgct cgaagggcgt cgagatcgtg ctcgactcgc cggtcgacga aatcatcgtc
    10261 gagcgcggcc gggccgccgg cgccgtcgcg ggcggcaaaa gctatcgggc caaggcggtg
    10321 gcggccaacg tcaatccgcg cttcctgttc gggagcctgc ttccggacgg ggccgtctcc
    10381 ccggaaatct cgctccgaat ggccggctgg gcggcggaat cggccacctt ccggatgaac
    10441 ctcgccctct ccgagctccc ccgcttcacc agcctgcccc aggcgggcga ccatctcacc
    10501 gccgggatca tcgtcgcgcc ctcgctcggc tacatggacc gcgcctatac cagcgcccgc
    10561 accgaaggct gggctcgaga gccgatcgtc gagatgctca tcccctccac cctcgacgac
    10621 agcctcgccc cgcccgggag ccatgtcgcg agcctgttct gccagcattt cccctaccgg
    10681 gtcgacggcg gctgggaggc gcgggtggac gaggccgccg accatatcgt cgcccatgtc
    10741 gatcgcttcg cgccgggctt caaagcctcg gtgatcggcc ggctcgccct ctcgccctgg
    10801 cacctcgagc agcgcttcgg aatcccgcgc ggcgacatct tccacggcaa gatgggtctc
    10861 gaccagctct tctccgcccg cccgatgctc ggccacgccg attaccggat gccgctcgcg
    10921 gggctctacc tgtgcggctc cggagcgcat cccggcggcg gcgtgaccgg cgccccgggc
    10981 cacaatgcgg cgcaggcgat tttggcggac cggaggaagt ggcggcgcgc ctgattcccg
    11041 tcataccggc gaagccggga cctcgtcgag aaaagggcgc cttgtccggc ctgaggcccc
    11101 ggctttcgcc ggggtgacgg ttcaatcccg cttcgtgctt cgctccgccg gctccgcttt
    11161 cgcccggcac cgttccgccg gcaccggctc cggatcctca tcggcctcga tcaaatagag
    11221 gagcggtcgc tcgacgtcga tgaccgcgcg gtgctccttg agcacgtcct ggccgatgat
    11281 tccgtgcacc gccccgcccg gcgccagcgg cgccagggca tcgccgatcg agcccagatc
    11341 ggtgaccacc atccgccgct gccgaagctt cacggcgccg aaggcgaggc tgtcgatgga
    11401 cacctgccgg gcgacattgg atccgccgag accggtggct ccccccggcc ggccgcgcgc
    11461 ggcggcaagc ccgaaatggg cgacatggtc cacatcgatc acggagaggt tggcgccggt
    11521 atcgaggatg aagacgccct ccttgccgtt gacggcggcg gtgacgaggt ggtggccggt
    11581 gccgcgcgcc tccctgagcg gaatggcggt gaagccgagc aggcaaaggg catggtccac
    11641 cgtccccgcc tcctccggcc tgtaatccac cttggcggct tccggcggcg gcggcgcgat
    11701 catgtcgcac gcggcgagcg ccaggggcag agcggcggca aggacgacct tcatcctcgc
    11761 gcaaatgcgg gctaagggcc ttcgttcctt caaggagaga ctcatgaaga cccgcgccgc
    11821 catcgccgtc gctccgaacc ggccgctgga gatccatgag gtcgacctcg aggggccacg
    11881 ggccggagag gtgctggtcg agatcatggc gaccggcgtc tgccacaccg acgcctacac
    11941 gcttgagggc aaagactcgg agggcatctt tccctcgatc ctcggccacg ag
//
LOCUS       DASWNU010000633         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_387070, whole genome shotgun sequence.
ACCESSION   DASWNU010000633 DASWNU010000000
VERSION     DASWNU010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387070"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGB57_12740"
     CDS             <1..191
                     /locus_tag="VGB57_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132258.1"
                     /translation="RADNLLDFERHGERTALEIPNTRIELVEGGHMLPFTQPELTAAF
                     VRRAAARQEEPHARRAAG"
     gene            169..>1883
                     /locus_tag="VGB57_12745"
     CDS             169..>1883
                     /locus_tag="VGB57_12745"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY0132259.1"
                     /translation="MPDALQADYIVVGAGSAGCVLANRLTEDGSSRVILLEAGGDDRP
                     LKQPSQFLSNVMIHVPVGYAATLKDPKVNWLFTTEPDAGTNGRTHVWPRGKVLGGSSS
                     INAMLYIRGQRADYDGWRQLGCEGWAWDDVLPYFRRSQHQERGACDIHGVGGPLNVSD
                     PTTGHEVSEAVIEACEALGLPHRDPNGAEQEGVGWYQVTIKDGKRCSAAVAYLHPAMD
                     RPNLQVETRALAHRILFEGKRAVGVEFVQNGVKRVARARAEVILAGGAVNSPQLLQLS
                     GIGPAALLREHGIEIVADLPGVGENLQDHFVTGETFRLKKGIVSVNELVRGTRFLKET
                     LKYMFGRKGLLTLSAAHVAVFCKSREGLAGPDIQFHILPATMDPDKLANAQKMELEKE
                     PGLTIAPCQLRPESRGTIRIKSPDPSAYPAIRPNYLSDRLDQEAIVAGLRWGRRIAEQ
                     PALARFIERELMPGPEAVTDEQLLDHARTWGTTIYHPVGTCAMGHGPQAVVDPRLRVH
                     GVEGLRVVDASVMPRLVSGNTNAPTIMIAEKASDLIRGNRAAAAACSGVVRRSGARSV
                     QPPLPG"
BASE COUNT          311 a          661 c          627 g          284 t
ORIGIN      
        1 cccgcgccga caatctcctc gatttcgagc gccacggcga acggacggcg ctcgagatac
       61 cgaacacccg gatcgagctg gtcgagggag gacacatgct accgttcacc cagccggagc
      121 tcaccgccgc tttcgtccgg cgcgccgccg cccggcagga ggaaccccat gcccgacgcg
      181 ctgcaggctg attatatcgt ggtcggcgcc ggctcggccg gctgcgtcct cgccaaccgg
      241 ctgaccgaag acgggagcag ccgggtcata ttgctcgaag cgggcggcga cgaccggccg
      301 ctcaagcaac cgtcgcaatt cctctccaac gtgatgatcc acgttccggt gggctatgcg
      361 gcgaccctca aggacccgaa ggtcaactgg ctgttcacga ccgagccgga cgccgggacc
      421 aacggccgca cccatgtctg gccgcgcggc aaggtgctcg gcggctcctc gtcgatcaac
      481 gccatgctct acataagggg ccagcgggcc gattatgacg gctggcgcca gctcggctgc
      541 gagggctggg cctgggacga cgtccttccc tatttccgcc gctcgcagca ccaggagcgc
      601 ggcgcctgcg atatccacgg cgtcggcggg ccgctcaacg tctccgatcc caccaccggc
      661 catgaggtgt cggaggccgt gatcgaggcc tgcgaggcgc tcggccttcc gcaccgcgat
      721 cccaatgggg cggagcagga aggtgtcggc tggtaccagg tgacgatcaa ggacgggaag
      781 cgctgctccg ccgccgtggc ctatctccac cctgcgatgg accgacccaa tctgcaggtc
      841 gagacaaggg cgctggccca ccggatcctg ttcgaaggca agcgcgccgt cggggtcgag
      901 ttcgtccaga acggggtgaa aagggtggct cgggcgaggg cggaagtgat cctggcggga
      961 ggcgccgtca attcgcccca gctgctccag ctctccggca tcggaccggc cgctttgctg
     1021 cgcgagcacg gcatcgaaat cgtcgccgac ctgccggggg taggcgaaaa cctccaggac
     1081 catttcgtca ccggcgagac ctttcgcctc aagaaaggca tcgtctcggt gaacgagctc
     1141 gtccggggca cgcggttcct caaggagact ctcaaataca tgttcggcag gaagggcctt
     1201 ctcacccttt ccgccgccca tgtcgccgtc ttctgcaagt cgcgggaggg cttggccggg
     1261 cccgacatcc agttccacat ccttcccgcg accatggatc cggacaagct cgccaacgcc
     1321 cagaagatgg agctggagaa ggagcccggc ctcaccatcg cgccgtgcca gctgcgcccg
     1381 gaatcgcgcg gcacgatccg gatcaagtcg cccgaccctt cggcctatcc cgcgatccgg
     1441 cccaattatc tctccgaccg gctggatcag gaggcgatcg tcgcgggcct gcgctggggc
     1501 cgccggatcg cggagcagcc ggcgctggcg cggttcatag agcgcgagct gatgcccggg
     1561 cccgaggcgg tgaccgacga gcagctgctc gaccacgccc gcacctgggg cacgaccatc
     1621 tatcatccgg tgggaacgtg cgcgatgggc cacgggccgc aggcggtggt cgacccgcgg
     1681 ctcagggtcc acggcgtgga ggggcttcgg gtcgtcgatg cgtcggtgat gccgcgtctc
     1741 gtctcgggca acaccaacgc gcccaccatc atgatcgctg agaaggctag cgacctgatc
     1801 cgcggcaacc gcgcagccgc cgccgcctgc tccggggtgg tccggcgttc aggggcccgc
     1861 tctgtgcagc cccctctccc cgg
//
LOCUS       DASWNU010000634         7551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_517097, whole genome shotgun sequence.
ACCESSION   DASWNU010000634 DASWNU010000000
VERSION     DASWNU010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517097"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VGB57_12750"
     CDS             <1..770
                     /locus_tag="VGB57_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0132260.1"
                     /translation="PWTRQGWKAQRGQKYLRSTLEAVVRERLPDGGPDDFLGKLIRGL
                     GDRFEPRQAAELAADNAMTFYLAGHETTANALTWTLYLLSEQPDLQDRVAAESARAVA
                     AGEGPDLPDRLPLLRTVLEESLRLYPPAPRFDRQAVEPDRLGDEAVQPGDIVSIWPWI
                     LHRHEALWERPDEFDPDRFAPGRREGRHRFQYIPFGAGPRLCVGARFATAEALTILAH
                     WLSRWSFAPVPGHEVRVHGMVTLRPAGGMPLTLSRRG"
     gene            967..3096
                     /locus_tag="VGB57_12755"
     CDS             967..3096
                     /locus_tag="VGB57_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0132261.1"
                     /translation="MYLRTISFLALASALAAPAAAAEEAAALDTAATDSESTIVVTGQ
                     RPEYGAQETCSATRTCTDVKDVPQSISVVSESQIEDQALRSIADVLMYVPGATPGTGE
                     GNRDQITLRGNNTTADFFVNGVRDDVQYFRDLYNAERIEILRGPNAMIFGRGGGGGIV
                     NRVTKRSSFRLYREFAGQGDSEGGFRLTGDLDQPLGDSVGLRLNGVYENGESFRRGVE
                     LERYGVNPVLGASFGDTRVDLSYEYFHDRRTADRGVPAFLGRPLRGFDRTFFGDPEQS
                     FARVDAHVVSLAVEHRFSDAATLRNRTSYGDYDKFYQNIFPGAVNAAGTQVALSAYND
                     TLKRQNLFSQTDLILEGDLGGLSHTFLLGFEIGRQDSVSRRRNGFFVSPANPAGVGSI
                     NVPLTSPTVDADLIFLPVNNNAGRTPSNFNEGDASILAFYAQEQLRISDHFEIVAGLR
                     FDRFKLEILNRNNGQTFSRTDNLLSPRLGLIVKPIPELSLYASYSRSYLPSAGDQFSS
                     LDVTSQSLKPERFDNYEVGAKWEPIPGLLATVALYRLHRTNTRALDPATNLTVLTGAQ
                     RSKGFEFGLERNITDKWQVSAGYALQKAEITRTTAAAPRGREVPLVPRHQFSVWNRYN
                     VTARLSFGLGVVAASKSYASISNAVTLPGYARVDAAVFYELADGIEAQVNVENLLGAD
                     YFATAHSDNNIAPGAPATARATLRFRF"
     gene            complement(3093..3497)
                     /locus_tag="VGB57_12760"
     CDS             complement(3093..3497)
                     /locus_tag="VGB57_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132262.1"
                     /translation="MKAVASSIACSILLSACAGPSARVAAAPSPAALAASAAVAEQIR
                     RCYRAPRVPSSGRNIVTRLFARYTADGMLVGVPLLVSQQGLTSESEPYASRMAEAAKL
                     AVMRCSRVRLPEELGTRRGSDFVLTFSPRRSA"
     gene            complement(3643..5145)
                     /locus_tag="VGB57_12765"
     CDS             complement(3643..5145)
                     /locus_tag="VGB57_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0132263.1"
                     /translation="MATRDDHGTYWNATAQSPGFPALSGDISVDVAVIGGGIVGVTTA
                     RLLKDRGLSVAVIEARRIGRQVTGKSTAKMSSQHGASHYQTLEKKFGEDRARLYAEAQ
                     EAGIRRISELAARHGIEADIEPMPAFVYTRDESYVSQLEKEVEVAKRLGLPASLTRDT
                     GLPFDVLQAMRWDDQAQFHPVRYVAGLAATLPGDGCHVFEDSRAVDWEPTRVVTERGT
                     VSARHVVMATHLPLGQVGMYYATNFPKAEPVIAAPIRRVPPGMYKNAEKPGHSIRTHR
                     NENGQVYGIAAGTNFKPGHPDEEREYLADIERWLTEHFEAGPIEYRWINEDYTPMDGA
                     PFIGWSSRSDGYLVATGFDAWGISNGTVAAEIIADLATGKENRWLEVFDATRVKPVAG
                     GPEFVKENASVAAHLVGGYLGSKAKSYDELGPGEAAILKIDGDNVAAFRDEQGGLHAV
                     SAVCTHMGCLVGWNDTDRTWDCPCHGSRFELSGEVIHGPATQPLGSKITG"
     gene            5338..5844
                     /locus_tag="VGB57_12770"
     CDS             5338..5844
                     /locus_tag="VGB57_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132264.1"
                     /translation="MKAISKTFLGAGAAAVAALSFAAPAEAQYRDRYRDRGGIDAGDI
                     ITGIAVLGGIAAITSAIGRDDSRYGYGYGNRYRGHYNYAVQACAQEAQRMSRGQVQIL
                     DVDRAGNDRYRVRGVVQAGYGGGYDRYDRGYSRGYDSRYERGYGSYGRDDFTCVARGN
                     GRITDFRL"
     gene            6030..>7551
                     /gene="guaA"
                     /locus_tag="VGB57_12775"
     CDS             6030..>7551
                     /gene="guaA"
                     /locus_tag="VGB57_12775"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY0132265.1"
                     /translation="MTVHPSETILIVDFGSQVTQLIARRVREAGVYSEIAPFSRAVEA
                     YERLRPKGIILSGGPSSVTLEDSPRADPSLFEAGVPMLGICYGQQLMAEQLGGRVVTG
                     DHSEFGRAFLEVQDDCALFDGLWAPGEKHQVWMSHGDKVDALPPGFRIVAQSPGAPFA
                     ATADEARRFYGLQFHPEVVHTPDGGRLIANFVRHVCGLGGDWTMAEFRATKIAEIREQ
                     VGGGRVICGLSGGVDSAVAAVLIHEAIGHQLTCVFVDHGLMRQSEAEQVVSLFRGHYN
                     IPLVHVDAQDLFLGGLAGLTDPEAKRKLIGKTFIDVFEEEARRIGGADFLAQGTLYPD
                     VIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRELGRELGLPDAFV
                     ARHPFPGPGLAIRIPGEVTKERCDILRKADAVYLDEIRNAGLYDAIWQAFAVLLPVRT
                     VGVMGDGRTYDYVCGLRAVTSTDGMTADVYPFDSTFLSRVATRIINEVKGINRVVYD"
BASE COUNT         1209 a         2670 c         2421 g         1251 t
ORIGIN      
        1 tcccgtggac ccggcagggc tggaaggccc agcgcggtca gaaatatctg cgctcgacgc
       61 tggaggcggt cgtccgcgag cggcttcccg acggcgggcc ggacgacttc ctcggcaagc
      121 tcatccgggg cctgggcgac cgcttcgagc cccgccaggc ggccgagctc gccgccgaca
      181 atgcgatgac cttctacctc gccggccacg agacgaccgc caatgcgctg acctggactc
      241 tctatctgct gtccgagcag cccgacctgc aggaccgcgt ggccgccgag agcgcgcggg
      301 ccgtggcggc tggagagggt ccggacctgc ccgaccgcct gccgctgctt cgcacggtgc
      361 tcgaggaatc gctccgcctc tatccgccgg cgccgcggtt cgaccggcag gcggtcgagc
      421 ccgaccggct cggcgacgag gcggtgcagc ccggcgacat cgtctcgatc tggccgtgga
      481 tcctccatcg ccacgaggcc ttgtgggagc gcccggacga attcgacccg gatcgcttcg
      541 ccccgggccg gcgggaaggg cgccaccgct tccagtacat cccgttcggc gccggaccgc
      601 ggctgtgcgt cggagcccgc ttcgccacgg ccgaggcgct caccatcctc gcccactggc
      661 tcagccgctg gagcttcgct ccggtgccgg ggcacgaggt gcgcgtccac ggcatggtga
      721 cccttcgtcc cgccggcggg atgccgctga cgctgtcgcg gcgggggtga ctgcagctct
      781 gccggctcct tcttcgtcac cctgaacttg tttcagggtc cagttctcca cccgcgaggg
      841 gaatccgggc tggatcctta aaccagttca ggatgacggc gcagccaaaa tgctattgcg
      901 aaacattctc aacaacgata ggccccgttc cggcgacgca atcatgcgcg ctgccaaggg
      961 gcccagatgt acctgcgcac gatttccttt ctcgcactcg cttccgcgct cgcggccccg
     1021 gcggcggccg ccgaggaggc agccgccctc gacaccgccg caaccgattc ggaatccaca
     1081 atcgtcgtca ccggccagcg ccccgaatat ggagcgcagg agacctgcag cgccacccgc
     1141 acctgcaccg acgtcaagga cgtgccgcag tccatctccg tcgtctcgga aagccagatc
     1201 gaggaccagg cgcttcgctc gatcgccgac gtcctgatgt acgtccccgg cgccacgccc
     1261 ggcaccggcg agggcaatcg cgaccagatc acccttcgcg gcaacaacac cacggccgat
     1321 ttcttcgtca acggagtccg cgacgacgtc cagtatttcc gcgacctcta caatgccgag
     1381 cgcatcgaga tcctgcgcgg ccccaatgcg atgatcttcg gccgcggcgg cggcggcgga
     1441 atcgtcaacc gggtgaccaa gcgctccagc ttccgcctct atcgcgagtt cgccgggcag
     1501 ggcgacagcg agggcggctt ccgactcacc ggcgacctcg accagccgct cggcgattcg
     1561 gtcggccttc gcctcaacgg cgtctacgaa aatggcgaga gcttccgccg cggcgtcgag
     1621 ctcgagcgct acggggtcaa tccggtgctc ggcgccagct tcggcgacac ccgggtcgac
     1681 ctgtcctacg aatatttcca cgaccgccgg accgccgaca ggggggtccc cgccttcctc
     1741 ggccggccgc tgcgcggctt cgatcggacc ttcttcggcg atcccgagca gagcttcgcc
     1801 agggtcgacg cccatgtcgt cagcctcgcg gtcgagcatc gcttctccga cgcggcgacg
     1861 ctcaggaacc gcacgagcta cggcgattac gacaaattct accagaacat cttccccggc
     1921 gcggtgaacg ccgccggcac ccaggtcgcc ctgtccgcct ataatgacac gttgaagcgg
     1981 cagaatctgt tcagccagac cgatctcatc ctcgagggcg atctcggcgg cctctcccac
     2041 actttcctgc tcggcttcga gatcggccgg caggacagtg tcagccgtcg ccgcaacggc
     2101 ttcttcgtct cgccggcgaa tccggcgggc gtcggatcga tcaacgtccc gctcacctcg
     2161 ccgaccgtcg acgccgacct catcttcctg ccggtcaaca acaatgcggg ccgaacgccg
     2221 tccaacttca acgaaggcga tgcctcgatc ctcgccttct acgcgcagga gcagctgcgc
     2281 atctctgacc atttcgagat cgtcgcaggc ctgcgcttcg accggttcaa gctcgaaatc
     2341 ctcaatcgca acaacggcca gaccttcagc cgaaccgaca atctgctgtc gccgcggctc
     2401 ggcctgatcg tcaagccgat cccggagctg tcgctgtatg cgagctacag ccgctcctac
     2461 ctgcccagcg cgggcgacca gttcagctcg ctcgacgtca cctctcagag cctgaagccg
     2521 gagcgtttcg acaattacga ggtcggcgcc aaatgggagc cgatccccgg cctgctcgcg
     2581 accgttgccc tctaccggct ccaccgcacc aacacccgcg ccctcgatcc ggcgaccaat
     2641 ctcaccgtcc tgacgggcgc tcagcgcagc aagggcttcg agttcggcct ggagcgcaac
     2701 atcaccgaca aatggcaggt ctcggccggc tatgcgctcc agaaggcgga gatcacccgc
     2761 accaccgccg cagcgcccag ggggcgggaa gtgccgctgg tcccgcgcca ccaattctcg
     2821 gtctggaacc gctacaatgt cactgcccgg ctaagcttcg gcctgggcgt cgtcgcggcg
     2881 tcgaaatcct atgcctcgat cagcaatgcc gtgaccctgc ccggctatgc gcgggtcgac
     2941 gcggccgtct tctatgagct cgccgacggc atcgaggccc aggtcaatgt cgagaatctg
     3001 ctcggcgccg actatttcgc gaccgcgcac agcgacaaca atatcgcccc cggcgccccg
     3061 gcgacggcgc gggcgacgct tcgcttccgc ttctaggcgc ttcggcgtgg actgaaggtc
     3121 aggacgaagt cgctgccgcg ccttgtgccc agctcctccg gcagccggac ccggctgcag
     3181 cgcatgacgg cgagcttcgc cgcttccgcc atccggctcg cataaggctc gctctcggac
     3241 gtcaggcctt gctgcgagac gagcagcggg acgcccacca gcatgccgtc ggccgtgtaa
     3301 cgggcgaaga gacgggtcac gatgttgcgg ccggaggacg gcactcgcgg cgctcggtag
     3361 cagcggcgaa tctgttccgc cacggccgcg ctcgcggcca gcgcggcggg cgacggcgcc
     3421 gccgccaccc gcgcggaagg tccggcgcag gcggaaagca ggattgaaca ggcgatggag
     3481 gaggcgacgg ctttcattcc agttacttag gaacggatac gcgttgtgga agggggtttt
     3541 cgccgccctc cccgtcatcc ccgcgaaagc cgggacctcg gggagacccg ctgtgcattt
     3601 tcgacctgag atccggcttt cgccgggacg acgggcttgc gactatcccg tgattttcga
     3661 tccgaggggc tgagtcgccg gtccgtggat gacctcgccg ctcagctcga aacgggagcc
     3721 gtggcacggg cagtcccacg tccggtcggt gtcgttccag ccgaccaggc agcccatgtg
     3781 ggtgcagacc gcggaaacgg catggaggcc gccctgctcg tcccggaagg cggcgacatt
     3841 gtccccgtcg atcttgagga tcgccgcctc gccgggtccg agctcgtcat aggacttggc
     3901 cttgctgccg agatagccgc cgacgagatg cgccgcgacc gaggcgttct ccttgacgaa
     3961 ttccgggccg ccggcgaccg gtttcacccg ggtcgcgtcg aacacctcga gccagcgatt
     4021 ctccttgccc gtggcgagat cggcgatgat ctcggcggcg accgttccgt tgctgatccc
     4081 ccaggcgtcg aagccggtgg cgacgagata gccgtccgat ctggacgacc agccgatgaa
     4141 gggcgctccg tccatcggcg tgtaatcctc gttgatccag cgatattcga tcgggccggc
     4201 ttcgaaatgc tcggtcagcc agcgctcgat gtcggcgaga tactcgcgct cctcgtcggg
     4261 gtggccgggc ttgaaattgg taccggcggc gatgccgtag acctggccgt tctcgttccg
     4321 gtgggtacgg atcgagtggc ccggcttctc cgcattcttg tacattccgg gcgggacccg
     4381 ccggatcggg gcggcgatca ccggctcggc cttgggaaag ttggtcgcat aatacattcc
     4441 cacctgcccg agcggcaaat gggtggccat gacgacatgg cgggccgaga ccgtgccccg
     4501 ctccgtcacc acccgggtcg gctcccaatc gactgccctg ctgtcctcga agacgtggca
     4561 gccgtcgccc ggaagggtcg cggccagccc ggcgacatat ctgaccggat ggaactgggc
     4621 ctggtcgtcc caccgcatcg cctgaagcac gtcgaaggga aggccggtgt cgcgggtcag
     4681 gcttgcggga aggccgagcc gcttcgcgac ctccacctcc ttctcgagct gcgacacgta
     4741 gctttcgtcg cgcgtataga cgaaggccgg catcggctcg atgtcggcct cgatgccgtg
     4801 ccgtgccgcg agctcggaga tccggcggat gccggcttcc tgggcctccg cgtagagccg
     4861 cgcccggtcc tcgccgaact tcttttcgag cgtctgataa tgggaggcgc cgtgctgcga
     4921 actcatcttc gcggtcgact tgccggtcac ctgccgcccg atccggcgcg cctcgatgac
     4981 ggcgacgctg agaccccggt ccttgagcag gcgcgccgtg gtgacgccca cgatcccgcc
     5041 gccgatgacc gcgacgtcga cgctgatatc gccggacagc gcggggaagc ccggcgactg
     5101 ggcggtcgcg ttccaatagg tcccgtggtc gtcgcgcgta gccatctcgc tctcctgcag
     5161 tctctctttc gatgccgcca cttcaactga gttcccggac cgctgttccg ggcggcgccc
     5221 ggcgaagctg aatattggcc gacagtccgg ttcaggacgg gctcagaccc gctcgggtag
     5281 acggtgagca tagcccacgc gtttctggtg gggcccacgt cgacaggaga taatgccatg
     5341 aaggccattt cgaagacatt tctgggcgcg ggcgccgctg ccgtcgccgc tctcagcttc
     5401 gccgcccccg ccgaggcgca atatcgcgac cgctaccgcg atcgcggcgg catcgacgcg
     5461 ggcgacatca tcaccggaat agccgttctc ggcggaatcg ccgccatcac ctcggcgatc
     5521 ggccgcgacg acagccgcta cggctatggc tacggcaacc ggtatcgcgg ccattacaat
     5581 tatgccgtcc aggcctgtgc gcaggaagct cagcgcatgt ctcgcggcca ggtccagatc
     5641 ctcgacgtcg accgtgccgg caacgaccgc taccgggtcc gcggcgtcgt ccaggccggc
     5701 tacggcggcg gctacgaccg ctacgaccgc ggctacagcc gaggctatga cagccgctac
     5761 gagcgcggct acggcagcta cgggcgcgac gacttcacct gcgtcgctcg cggcaacggc
     5821 cggatcaccg acttccgcct gtaacccgcg gttgaatgcg taaggatggg cgcgggcgaa
     5881 agtccgcgcc cattgctttt ggggccgtca tcccggcgga agccgggacc gcaggtcgga
     5941 aagtctcggc ggtctctccg aggtcccggc tttcgccggg atgacgaggg tctctttaca
     6001 cgccgccgcc gccgcgctta tcggccttca tgaccgtcca cccttccgaa accatcctga
     6061 tcgtcgattt cggaagccag gtgacccagc tcatcgcgcg gcgggtgcgc gaggccggag
     6121 tctatagcga gatcgcgccg ttcagccgcg cggtagaggc ttatgagcgg ctgagaccca
     6181 aaggcatcat cctctccggc ggcccctcgt cggtcacgct agaggacagc ccgcgggccg
     6241 atccttcgct gttcgaggcg ggagtgccga tgctcggcat ctgctacggc cagcagctca
     6301 tggccgagca actgggcggc agggtcgtca ccggcgatca cagcgagttc ggccgcgcct
     6361 tcctcgaagt ccaggacgat tgcgccctgt tcgacggcct ctgggcgccg ggcgagaagc
     6421 accaggtgtg gatgagccac ggcgacaagg tcgacgctct gccgcccggc ttccggatcg
     6481 tcgcccagtc gcccggagcg cccttcgcgg cgacggcgga cgaggcgcgc cgcttctacg
     6541 ggctgcaatt ccacccggaa gtcgtccaca cgcccgacgg cggcaggctg atcgccaatt
     6601 tcgtgcgcca cgtctgcggc ctcggcggcg actggacgat ggccgagttc cgggccacca
     6661 agatcgccga gatccgcgag caggtcggcg gtggccgggt catttgcggc ctttcaggcg
     6721 gagtcgacag cgccgtcgcc gcggtcctta tccacgaggc gatcggccac cagctgacct
     6781 gcgtcttcgt cgaccacggc ctgatgcggc agagcgaggc cgagcaggtc gtgtcgctgt
     6841 tccgcggcca ttacaacatc ccgctcgtcc acgtcgacgc ccaggacctg ttcctcggcg
     6901 gcctcgccgg gctcaccgac cccgaggcca agcgcaagct catcggcaag accttcatcg
     6961 acgtcttcga ggaagaggcg cgaaggatcg gcggcgccga tttcctggca cagggcacgc
     7021 tctatccgga cgtgatcgag agcgtcagct tcaccggcgg accttcggtg acgatcaaga
     7081 gccaccataa tgtcggcggc ctgcccgagc ggatgaacat gaagctggtc gaaccgctca
     7141 gggaattgtt caaggacgag gtgcgcgagc tcggccgcga gctcggcctt cccgacgcct
     7201 tcgtcgcacg ccaccccttt cccggcccgg gcctcgccat caggatcccg ggcgaggtga
     7261 cgaaggagcg ttgcgacata ttgcgaaagg cggacgcggt ctatctcgac gagattcgca
     7321 atgccggcct ctacgacgcc atctggcagg ccttcgcggt gcttttgccg gtgcgcacgg
     7381 tcggagtgat gggcgacggg cggacatacg attatgtctg cggccttcgc gccgtgacct
     7441 cgaccgacgg catgaccgcc gacgtctacc cgttcgactc caccttcctc agccgggtcg
     7501 ccacgcgcat catcaatgag gtcaagggga tcaaccgggt ggtctacgac t
//
LOCUS       DASWNU010000635         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_50827, whole genome shotgun sequence.
ACCESSION   DASWNU010000635 DASWNU010000000
VERSION     DASWNU010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50827"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..680)
                     /locus_tag="VGB57_12780"
     CDS             complement(33..680)
                     /locus_tag="VGB57_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEY0132266.1"
                     /translation="MRPNPKLALALGLGALALAGCGTAGARTGNRLPPASFVGSDDTA
                     ASERYVIGALDQLNVFVWRNADLGGKVQVRPDGMITTPLIPDLQAAGKTPAQLADDIR
                     LALTKYVDDPRVTVMVETFQGSFSQQIRVVGATEKPASLPYRANMTLLDAMIAVGGLS
                     EYAAGDKARLVRHDKATGTQTEYDLKIARLLKKGDTKANVRLEPGDVIIIPESMF"
     gene            complement(935..2164)
                     /locus_tag="VGB57_12785"
     CDS             complement(935..2164)
                     /locus_tag="VGB57_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HEY0132267.1"
                     /translation="MSKPMGAIPDGFESEDGMLLIGGRRADSLVEEAGDTPLFVYDLS
                     RVTAQVQRFRSAFPGVNLHYAIKANTYDPLLRHVAKLVDGLDVASAGEMAAAMGSGTD
                     PREISFAGPGKRDDELAAAIEAGVTLNLESEGEADRALAIGRAQGRTVRLAVRVNPDF
                     EIKGSGMRMGGGAKPFGVDAARVPALIRKVVESGGMWRGLHIFAGSQALSAQSLIEAQ
                     RATVALAAELAEAVGIAPPLVNLGGGFGIPYFATEAPLDVEAVGAALAETLRDRPAIL
                     ATSGFAIELGRWLVGEAGVYLTRIVDRKESHGKTFLVTDGGMHHQLAASGNFGQVVRR
                     NYPVAIASRFGAPQEEEASIVGCLCTPLDRLADDVTIPSAGVGDLVAVFLAGAYGLSA
                     SPAEFLGHPPAREMVVG"
     gene            complement(2161..3675)
                     /locus_tag="VGB57_12790"
     CDS             complement(2161..3675)
                     /locus_tag="VGB57_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HEY0132268.1"
                     /translation="MQAPDPTPRPLDHLASMGDAGAPALLTREGLLDYAGLEAAVGTL
                     AAALKARGFAPGDRVASWLPKTRMTGLLPLACARAGLVHVPVNPLLKRAQVGHILADS
                     GARLLITGKARLGTLGEGDLPAGCAAAMEEEGAAMLAGNERLPPSGADPEDLAALLYT
                     SGSTGRPKGVMLSHANMWLGAVSVAHYLRLAPDDRVLGVLPLSFDYGQNQLLSTWAAG
                     ACVVPIEYLAARDVIRAVETHGITTLAGVPPLWVQLVEAPWPHRAALSLRRLTNSGGR
                     LPVSIVRRLRELFPAAELYLMYGLTEAFRSTYLEPALLEEHPESIGTAIPFAEVMTSK
                     PDGTLCGPGEDGELVHAGPLVAKGYWNDPERTAVRFKPAPKGSLYGGTAVWSGDRVRQ
                     DEQGLLYFVGREDSMIKTSGNRVSPTEVEEAAVASGVVVEAVALGVPDDRLGEAIALV
                     VRGRGQEADVDLRNFLRRELPNFMQPAHIIWRDELPRSPNGKLDREVIKSELTQ"
BASE COUNT          595 a         1276 c         1353 g          621 t
ORIGIN      
        1 gcgagcgggg ttggggcggc ggcgggtgcg gctcagaaca tgctttcggg gatgatgatg
       61 acgtcgcccg gctcaagccg gacgttggcc ttggtgtcgc ccttcttgag aagccgggcg
      121 atcttgaggt cgtattcggt ctgggtgccg gtggccttgt cgtggcgcac caggcgcgct
      181 ttgtcgccgg cggcatattc ggacaggccg ccgaccgcga tcatcgcgtc gagcagggtc
      241 atgttggcgc ggtagggaag cgaggccggc ttctcggtcg ctccgacgac ccggatctgc
      301 tggctgaagc tgccctggaa ggtctccacc atcaccgtca cccggggatc gtcgacatat
      361 ttggtcagag ccaggcggat gtcgtcggcg agctgcgccg gagtcttgcc cgccgcctgc
      421 aggtccggga tcagcggggt ggtgatcatc ccgtcggggc ggacctggac cttgccgccc
      481 agatcggcat tgcgccagac gaagacgttg agctggtcga gcgccccgat gacatatctc
      541 tcgctggcgg cggtgtcgtc ggagccgacg aagctggccg gcggaagccg gttgccggtc
      601 cttgcgccgg ccgtgccgca gcccgcgagg gcgagggcgc cgaggccgag ggcgagagcg
      661 agcttgggat ttggacgcat cgaactgatc ctcttctggt cgccgcggtt cgcaggcctg
      721 acgtcaggca cgcgaaattt gggtccgcta tggcgtgaag aggtaaaatt accgttagga
      781 acaatggcct ttagaggcgc gtcccggaat gggactggcg tcagtgagga gttaatccgc
      841 cttgtcgtca tcccggactt gatccgggat ccaccttttc ctgagccgct gccgaggaag
      901 aaggtggatg ccggatcaag tccggcatga cgggtcagcc gaccaccatc tcccgcgccg
      961 gcgggtgccc caggaactcc gccgggctcg ccgaaagccc gtaggcgccg gcgaggaaga
     1021 ccgcgacgag atcgcccacc ccggcgctcg ggatcgtgac gtcgtccgcg aggcggtcga
     1081 gcggcgtgca caggcagccg acgatgctcg cttcctcctc ctgcggagcg ccgaagcggg
     1141 aagcgatcgc gaccggataa ttgcgccgaa ccacctggcc gaagttgccc gaggcggcga
     1201 gctggtggtg catgccgccg tcggtgacga ggaaggtctt gccgtggctc tccttgcggt
     1261 cgacgatccg ggtcagataa actcccgcct cccccaccag ccagcggccg agctcgatcg
     1321 cgaagccgct tgtcgcgagg atcgcgggcc ggtcgcgcaa cgtctcggcc agggccgcgc
     1381 cgaccgcttc gacgtcgagc ggtgcctcgg tggcgaaata gggtattccg aagccgccgc
     1441 cgagattgac cagcggcgga gcgatgccga ccgcctcggc cagctcggcg gcgagcgcca
     1501 cggtcgcccg ctgcgcttcg atcaaggatt gcgcggacaa ggcctgggaa ccggcgaaaa
     1561 tatgcagtcc cctccacatt ccgccacttt cgaccacttt tcggatcaag gccgggacgc
     1621 gcgccgcgtc gaccccgaac ggcttggcgc cgccgcccat ccgcatgccc gaacccttga
     1681 tctcgaagtc gggattgacc ctcaccgcca gccgtacggt tcggccctgg gcgcggccga
     1741 tcgccagcgc ccgatccgcc tcgccttcgg attcgaggtt gagtgtgacc ccggcctcaa
     1801 tcgcggcggc aagctcgtcg tcgcgcttgc cgggaccggc gaacgagatt tcgcgggggt
     1861 ccgtgcccga tcccatcgcc gccgccattt cgcccgccga cgccacgtcg aggccgtcga
     1921 ccagcttcgc aacgtgtctc agaagtggat cgtaggtatt tgcttttatc gcataatgca
     1981 gatttacgcc gggaaaagcc gagcgaaagc gctggacctg ggcggtgacc ctggagagat
     2041 cgtagacgaa caggggcgtg tccccggcct cctcgacgag cgagtcggcg cggcggccgc
     2101 cgatcagcag cattccgtcc tcgctctcga agccgtccgg aatcgcgccc atgggcttgc
     2161 tcattgagtc agctcgctct tgatgacttc gcggtcgagc ttgccgttgg gagagcgcgg
     2221 cagctcgtcg cgccagatta tatgagccgg ctgcataaaa ttcggtaact cgcggcgaag
     2281 aaagttgcgc aggtcgacat cggcctcctg acccctgccc cgaacgacca aagcgatcgc
     2341 ctcgccgagc cggtcgtccg gcacgcccag cgccaccgcc tcgacgacga ctccgctcgc
     2401 caccgcggct tcctcgacct ccgtcgggct gaccctgttg ccgctggtct tgatcatcga
     2461 atcctcgcgg ccgacgaaat agagcaggcc ttgctcgtcc tgccggaccc ggtcgccgga
     2521 ccagacggcc gtgccgccgt aaagcgagcc cttcggcgcc ggcttgaagc ggacggcggt
     2581 ccgctccggg tcgttccaat agcctttggc gacgagcggg ccggcgtgga ccagctcgcc
     2641 gtcctcgccc gggccgcaga gcgtgccgtc gggctttgac gtcatgactt cagcgaaggg
     2701 tatggcggtg ccgatcgact ccggatgctc ttcgagcagg gccggctcca gataggtcga
     2761 gcgaaaggct tcggtgaggc cgtacatcag atagagctcg gcggccggga aaagctcgcg
     2821 caggcggcgg acgatcgaca cgggcagccg gccgccgctg ttggtgagtc ggcggagcga
     2881 caatgcggcc ctgtgcggcc agggcgcctc gaccagctgg acccagagcg gcggcactcc
     2941 ggccaatgtg gtgatgccat gggtctcgac ggcgcggatg acgtcgcggg cggcgagata
     3001 ttcgatcgga accacgcagg cgccggccgc ccaggtcgag agcagctggt tctggccgta
     3061 gtcaaagctg agcggaagga ctcccagcac ccgatcgtca ggcgccagcc ggaggtaatg
     3121 cgcgacgctc accgccccca gccacatatt ggcgtggctg agcatcacgc ccttgggccg
     3181 cccggtcgag ccggacgtat agagcaaggc ggcgagatct tcgggatcgg cgccggaagg
     3241 cggcaggcgc tcgttgccgg cgagcatggc ggcgccttcc tcctccatcg ccgcggcgca
     3301 gccggcgggc aggtcgccct ccccgagggt cccgaggcgc gccttgcccg tgatcagaag
     3361 cctggcgccg ctgtcggcga ggatatgccc gacctgcgcc cgcttgagca gcgggtttac
     3421 cggcacgtgg acgagcccgg cccgggcgca ggcgagcggc agaaggccgg tcattcgggt
     3481 cttggggagc cagctcgcca cccggtcgcc cggagcgaag cctcgcgcct tcagcgccgc
     3541 ggcgagcgtc ccgaccgcgg cctcgaggcc ggcataatcg agcagcccct ccctggtcag
     3601 aagggccgga gcaccggcat cgcccatcga agcgaggtgg tcgaggggac ggggggtcgg
     3661 atcgggggct tgcaccgtgg ctcctgtgtg cgccacagca ggcacgctgg gcttggtgcc
     3721 gggggatagc gcgttgggct tcgaggtgga actgttcgat agcctcgact cggcggcggc
     3781 ggattccggc ggcgccctcg accgcccgcc ttctctgttc gaccggctcg gctggttccg
     3841 gctgg
//
LOCUS       DASWNU010000636         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_284044, whole genome shotgun sequence.
ACCESSION   DASWNU010000636 DASWNU010000000
VERSION     DASWNU010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284044"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VGB57_12795"
     CDS             <1..1509
                     /locus_tag="VGB57_12795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0132269.1"
                     /translation="LIEQNGSASYVREPLALIIAPTRELALQVSRELIWLYGKVGARV
                     ATCVGGMDASKERRNLQQGAHIVVGTPGRLRDHLERGALDLSNLRVAVLDEADEMLDM
                     GFREDLEEILDATPPARRTLLFSATMPRPIVALAKRYQKDALRISTVGEERGHGDIAY
                     QAMTVAPADIEHSVVNLLRLHEAESALLFCATRENVRRLHSSLVERGFSAVALSGEHS
                     QNERNQALQALRDGRARVLVATDVAARGIDLPNLSLVVHVELPRDAETLQHRSGRTGR
                     AGRKGIAVLVVPYPRRKRVEMVLRGARIEVEWIKPPTPEDIRKSDHDRLLAQLLEPAE
                     IEEEDRDLARRLMEAKPPEEIAAALVRAHRARMPAPEEMVDSGSAGEVRRPEGPRPGF
                     EDTIWFRMDIGRRHNADPRWLLPLLCRRGHITKSEIGAIRIAAGETMFEVPRAAAGRF
                     MAAVRRTATDEDGEGGVRIEPVEGKPRDAARQNRRAGPPPQKRYKARPAGRG"
     gene            1681..2022
                     /locus_tag="VGB57_12800"
     CDS             1681..2022
                     /locus_tag="VGB57_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132270.1"
                     /translation="MAITTIADIMTWLLAAWLFLAMGIILFHMLSGHIILTGLLKLEP
                     RAPFGFDRLQLVAVTLLFAAGYVVAALYRGPGDTLPDIPTPLLLILIGSNGTYLAVKA
                     TTIGRISKRGK"
     gene            2022..2477
                     /locus_tag="VGB57_12805"
     CDS             2022..2477
                     /locus_tag="VGB57_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132271.1"
                     /translation="MPTNIWTWISAGVAIALAALIMSFAIVIIIKLFTGAIDLRWLLA
                     EPAGPIQPGAHPDEPGTQPKASISRFQFLIFTFVIAGVYLVLCLESGRFVEIPENVIL
                     LLGVSGTSYAASKGIKAAADTSQRNAAVTTGGDPIQAASPRPAGPNANG"
BASE COUNT          403 a          885 c          862 g          381 t
ORIGIN      
        1 ctgatcgagc agaacggaag cgcctcttat gtgcgcgagc cgctggcgct gatcatcgct
       61 cccacccgcg agcttgcatt gcaggtgagc cgcgagctga tctggctcta tggcaaggtt
      121 ggcgctcggg tggcgacctg cgtcggcggc atggatgcgt ccaaggagcg ccgcaacctc
      181 cagcagggtg cgcacatcgt cgtcggcacg cccggacgtc tccgcgacca tctggagcgg
      241 ggcgcgctcg atctttccaa cctccgcgtc gccgtcctcg acgaagccga cgagatgctc
      301 gacatgggct tcagggagga tctcgaggag atactcgacg cgaccccgcc ggcccggcgg
      361 accctgctct tctcggcgac catgccgcgg ccgatcgtgg cgctcgccaa gcgttaccag
      421 aaggatgccc ttcgcatctc gacggtcggc gaggagcgcg gccatggcga catcgcctat
      481 caggcgatga ccgtcgcgcc cgccgacatc gagcattcgg tggtcaacct gctccggctg
      541 cacgaggcgg agagcgccct gctgttctgc gccacccgcg agaatgtccg gcggctccat
      601 tcgtccctgg tcgagcgcgg cttcagcgcc gtggctttgt cgggggagca tagccagaac
      661 gagcgcaacc aggcccttca ggcgcttcgc gacgggcggg cgagggtgct ggtcgcgacc
      721 gacgtcgccg cgcgcgggat cgacctgccc aatctcagcc tggtcgtcca tgtcgagctg
      781 ccgagggacg cggaaacgct ccagcaccgc tcgggccgga ccgggcgcgc gggacgcaag
      841 gggatcgccg ttctcgtcgt cccctatccg aggcgcaagc gggtcgaaat ggtgcttcgc
      901 ggcgcacgca tcgaggtcga gtggatcaag ccgccgactc cggaggatat ccggaagagc
      961 gaccacgacc ggctgctcgc ccagctgctc gaaccggccg agatcgagga ggaggatcgc
     1021 gacctcgccc gccggctgat ggaggcaaag ccgcccgagg agatcgccgc ggcgctcgtg
     1081 cgggcgcacc gcgcccggat gcccgcgccc gaggagatgg tcgattcggg aagcgcgggc
     1141 gaggtccgcc ggcccgaggg gccgcgcccc ggcttcgagg atacgatctg gttccggatg
     1201 gacataggcc ggcgccacaa tgccgacccg cgctggctcc tgcctttgct ctgccggcgc
     1261 ggccacatca ccaagtccga gatcggcgcc atccggatcg ctgcgggcga gacgatgttc
     1321 gaggttccgc gcgccgcggc cggccgcttc atggcagcgg tccggcgcac cgcgaccgac
     1381 gaggacggcg agggcggggt gcgaatcgag ccggtcgagg gcaagccccg cgacgccgcc
     1441 cgtcagaacc gccgggccgg acctccgccg cagaagcgct acaaggccag gccggccggg
     1501 cgaggctgac ggagaaggcc gagccttgcc ggccgccccc ccgatcgccg gtgcgcggac
     1561 ggggcgggcg gaacgtcacg tttaatcgag tgactcgccg gggccgtaat gataaatcgc
     1621 ttttaacggc gcggcgtagg ggctgcgggt aagcaggcgg cgccgggacc ggggggctcg
     1681 atggcgatca cgacaatcgc cgacatcatg acgtggcttc tcgccgcgtg gctgttcctc
     1741 gccatgggga tcatcctgtt ccacatgctg agcggccaca tcatactgac cggcctgctc
     1801 aagctcgaac cgcgcgcgcc gttcgggttc gaccggctgc agctcgtcgc cgtcaccctg
     1861 ctctttgccg ccggctatgt cgtcgccgcc ctctatcgcg ggcctggaga cacattgccg
     1921 gacattccga ctccgctgct gctcatcctc atcggaagca acggcacata tctcgcggta
     1981 aaagccacca cgatcgggcg catcagcaag aggggaaagt aatgcctacg aacatctgga
     2041 cctggatttc ggcaggggtc gcgatcgctc tcgcggcgtt gataatgtcc ttcgcgatcg
     2101 tgatcatcat caagctgttc accggcgcga tcgacctgcg ctggctcctc gcggagccgg
     2161 cgggcccgat ccagcccggc gcccaccccg acgaacccgg gacccagccc aaggcgagca
     2221 tctcgcgctt ccagttcctc atcttcacct tcgtcatcgc cggggtctat ctcgtcctgt
     2281 gcctcgaatc gggccggttc gtggagatac cggagaacgt catcctcctt ctcggcgtca
     2341 gcggtaccag ctacgccgca tcgaagggga tcaaggccgc cgccgacacc agccagcgca
     2401 acgcggcggt gacgaccgga ggggatccca tccaggccgc ttcgccgcgc ccggccgggc
     2461 ccaacgccaa tggctaggct tcccgtcccg ggtcggcggg ccggccgcgg cgggaagagc
     2521 gccgccaccg c
//
LOCUS       DASWNU010000637         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_128592, whole genome shotgun sequence.
ACCESSION   DASWNU010000637 DASWNU010000000
VERSION     DASWNU010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128592"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VGB57_12810"
     CDS             complement(<1..464)
                     /locus_tag="VGB57_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY0132272.1"
                     /translation="MTTDAANGRNGFGGWKTAAAAGLLGALVGGGAVAYVSRESVRDY
                     LLENPEVLPLAMDRLREREATRVISANRASLETPFAGAWAGAADGDVVLVEFFDYACG
                     YCRKSNSDIERLLREDPRLKVVWREWPVLGPDSESAAEVSMAAARLGKFKPF"
     gene            complement(454..1845)
                     /locus_tag="VGB57_12815"
     CDS             complement(454..1845)
                     /locus_tag="VGB57_12815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0132273.1"
                     /translation="MAEASVTLRRWSAFAARLAFLLILTFGLTARPAAAQSILRDAET
                     EALFKDMSRPIVAAAGLRPEDVQVVLIHDRSINAFVAGGQIVYIHSGLIAAADSANEV
                     QGVIAHEVGHITGGHVIRIQEGAKVATGIMLLSMLLGAAAMAAGAGEAGMGVMAAGQQ
                     AALGKFLAFSRTQESSADAAAAGFLAKSRTNGKGLLTFFKKLQNQEFRYAIPQDNGYM
                     RTHPLTGERLAALEDSVKSAPGWNSAPDARLEAKFQRVKAKLSGYVEDPTRTRVAYPE
                     SNKSVPARYARAYAWHKSAYPDKALAEADSLLADAPTDPYFHELKGQILLESGRPNEA
                     LESLRQSVRYAPDQPLISALLGHALIATERPDHYEEAKRVLRAAIGRDNSNPFAWYQL
                     GIVYDREGDPGRAALATAERYNLQGEPRLALVNAEQALKTVPSGTPDWLRAQDIAMVS
                     RTEVEKKKKNRDN"
     gene            complement(1923..>3447)
                     /locus_tag="VGB57_12820"
     CDS             complement(1923..>3447)
                     /locus_tag="VGB57_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY0132274.1"
                     /translation="KLLMPSHVKRVQNYVDTVPLFQRYGAEDQLAAMYQPVVQLKSGG
                     YLVINPTEALVSIDINSGRSTREHNIEQTAYATNIEAANEIARQLRLRDMAGLVVIDF
                     IDMESNGHIRKVEKAMKEALKNDRARIQVGRISSFGLMEMSRQRLRTGVLEASTRPCP
                     HCEGSGLMRTASSAGLSALRMLEEEAARGRGSRIVLRAGREAAVYVLNRKRGEIGEIE
                     SRYGVVIEVLIEETFEGAKMAVESFGPPPAARPRVERIVEPEVDEEEFAEDLAEEIDD
                     EEEEAEESEEESRPRRPESREAREREESEDRGRKRRRRRGRRGGRRREGEEGGDHGAS
                     EHDAPREVAQVPGEAEPQYAPEPEAVVAVEPAPEAPAETEEKPKGRRRPRARRKDEAP
                     APAVEAAPEPEVIEAPAAAAEAEPAPVTEAAPSAEDEKPAKPKRTRRKKAPEADSPPE
                     APAEAPAPEAAPEAANDRDGTAAVEPVETGEAAEAAEAEAAEAGEPRRGWWQRTFGA"
BASE COUNT          452 a         1183 c         1203 g          609 t
ORIGIN      
        1 tggaagggct tgaatttccc tagccgtgcg gcggccatgc tgacctcggc ggcgctctcg
       61 ctgtccggcc cgagcaccgg ccattcgcgc cacaccacct tgagcctggg atcctcccga
      121 agcagccgct cgatgtccga attgctcttc cggcaatagc cgcaggcata atcgaagaac
      181 tcgacgagga cgacgtcgcc gtccgcggcg cccgcccagg cgcccgcgaa cggggtttct
      241 agcgaggcac ggttcgcgga gatcacccgg gtcgcctcgc gctcgcgcaa ccggtccatc
      301 gcgaggggaa gcacttccgg attttcgagc aggtaatcgc ggaccgactc gcgcgacaca
      361 taggcgaccg cgccgccgcc gacgagcgcg ccgaggagcc ccgccgccgc cgccgtcttc
      421 cagcctccga agccgttgcg cccgttggcc gcgtcagttg tcacgatttt ttttcttctt
      481 ttccacctcg gtgcgcgaaa ccatggcgat gtcctgggcg cgaagccagt cgggcgtgcc
      541 gctgggaacg gtcttcagcg cctgctcggc attgacgagg gcgaggcggg gttcgccctg
      601 gagattgtag cgctcggcgg tggcgagcgc ggcccggccg ggatcgcctt cgcggtcgta
      661 gacgatgccg agctgatacc aggcgaaggg gttcgaattg tcgcggccga tcgcggctcg
      721 aagcacgcgc ttcgcctcct cataatggtc cggcctttcg gtggcgatga gcgcatggcc
      781 gagcagggcc gagatgagcg gctggtcggg cgcatatctg accgactggc gaagcgattc
      841 gagggcttcg ttcggacggc ccgattcgag gaggatctgg cccttcagct cgtggaaata
      901 gggatcggtc ggagcgtcgg cgagaaggct gtccgcttcg gcgagcgctt tgtccggata
      961 ggcgctcttg tgccaggcat aggcccgggc atagcgggcc ggaacgctct tgttgctctc
     1021 cggataggcc acgcgcgttc gcgtcgggtc ttcgacatag cccgaaagct tcgccttgac
     1081 gcgctggaac ttggcttcga gcctggcgtc gggagcgctg ttccagccgg gcgcgctttt
     1141 caccgaatcc tcgagcgcgg cgagccgctc cccggtcagc ggatgggtgc gcatatagcc
     1201 attgtcctgg ggaatggcgt agcggaactc ctggttctgg agcttcttga agaaggtgag
     1261 caggcccttg ccgttggtcc gcgacttggc gaggaagccg gccgcggcgg cgtccgccga
     1321 ggattcctgg gtgcggctga aggcgaggaa cttgccgagc gccgcctgct ggccagcggc
     1381 catcacgccc atgcccgcct cgcccgcgcc ggccgccatc gccgcggctc cgaggagcat
     1441 ggagaggagc atgatcccgg tggcgacctt cgcgccttcc tggatgcgga tgacgtggcc
     1501 gccggtgatg tggccgacct cgtgggcgat gacgccctgg acctcgttgg cggaatccgc
     1561 ggcggcgatc agcccggagt ggatgtagac gatctggccg cccgccacga aggcgttgat
     1621 cgaccggtcg tggatgagga cgacctggac gtcctccggc ctcagcccgg cggccgcgac
     1681 gatcgggcgc gacatgtcct tgaacagcgc ctcggtctcc gcgtcgcgaa ggatcgactg
     1741 cgccgccgcg ggccgggccg tcaggccgaa ggtcaaaatg aggaggaatg cgaggcgggc
     1801 ggcaaaggcg ctccaccttc tcagcgtaac actagcttca gccattccgc atcctatccc
     1861 tttgcggggc tgaacgtgac atgagcgcgc ccgcttccgc aagcgcgctc atgccctgtt
     1921 cgtcaggccc cgaaagtgcg ctgccaccag ccgcggcgcg gttcgcccgc ctcggcggct
     1981 tcggcttcgg cggcttcagc ggcttcgccg gtctcgacag gctcgacagc cgccgtcccg
     2041 tcgcggtcgt tggccgcctc gggcgccgcc tcgggcgccg gagcttcggc cggagcctcc
     2101 ggcggactgt ccgcctccgg ggccttcttg cggcgggtcc gcttcggctt ggcgggcttt
     2161 tcgtcttcgg cggagggagc ggcttccgtc acgggagccg gttccgcctc ggcggccgcg
     2221 gccggagctt cgatgacttc gggctcggga gcggcctcga ccgccggagc cggagcttcg
     2281 tccttgcggc gggcacgggg acggcggcgg cccttgggct tctcctccgt ctcggccgga
     2341 gcctccggag caggctcgac cgccaccacg gcctccggct cgggcgcgta ttggggctcc
     2401 gcttcccccg gcacctgggc cacttcccga ggagcgtcgt gctcggaggc gccgtggtcg
     2461 ccgccttcct cgccttccct ccggcggccg ccgcgacggc cgcgccggcg ccggcgcttg
     2521 cgcccgcggt cctcgctctc ctcgcgttca cgggcttcgc ggctttccgg acggcgggga
     2581 cgggactctt cctcgctctc ctccgcctcc tcttcttcgt cgtcgatctc ctcggcgaga
     2641 tcctcggcga actcttcctc gtccacctcg ggctcgacga tgcgctcgac gcgggggcga
     2701 gccgcgggcg gcgggccgaa ggattcgacc gccatcttgg cgccttcgaa tgtctcctcg
     2761 atcagcacct cgatgaccac gccgtagcgc gattcgatct cgccgatctc gccgcgcttc
     2821 cggttgagca catagaccgc cgcctcgcgg ccggcgcgga gcacgatccg gctgccgcgg
     2881 ccgcgcgcgg cttcctcctc gagcatgcgc agcgccgaga gtccggccga cgacgcggtg
     2941 cgcatcaggc cgctgccctc gcaatgcggg caggggcggg tcgaggcctc gagcactccg
     3001 gtgcgaagcc gctggcggct catctccatc agcccgaagg acgagatccg gccgacctgg
     3061 atccgggccc ggtcgttctt cagcgcctcc ttcatcgcct tctcgacctt acggatatgg
     3121 ccgttcgatt ccatgtcgat gaagtcgatg acgacgaggc cggccatgtc gcgcaggcgg
     3181 agctggcggg cgatctcgtt ggcggcctcg atgttggtcg cgtaggcggt ctgctcgata
     3241 ttgtgctcgc gggtcgagcg gccggagttg atgtcgatcg agaccagggc ctcggtcggg
     3301 ttgatgacca gatagccgcc cgatttgagc tggacgacgg gctggtacat ggccgcgagc
     3361 tggtcctcgg cgccgtagcg ctggaagagc ggcacggtgt cgacgtaatt ctgcacccgc
     3421 ttcacatggc tgggcatgag cagcttc
//
LOCUS       DASWNU010000638         7504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_76428, whole genome shotgun sequence.
ACCESSION   DASWNU010000638 DASWNU010000000
VERSION     DASWNU010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76428"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VGB57_12825"
     CDS             complement(<1..1046)
                     /locus_tag="VGB57_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132275.1"
                     /translation="MLLTGADAPPRRTETWYRIVAADGTAIGHVRSEARQAAGGRETV
                     SEQQMLVDSGEGITRISERRIVREDPSGRPVSISEYSRIGGSWSRVEARIGPDQAEIV
                     RSTAAGRTVATVPLPPDVRFDSGSGLLRGWNRIKAPRLEFDNLAVAATAVEHVVIEPD
                     PATANADGSFSVIRRRFEGHELRSVGRLTLAPDGRVRSTTQPMMGTSVTIELSDRETA
                     LKPHSPYRLLQSAMVKSPFRISPEAMRGRIRYRFAYRDSLAFPLPETGEQRAVASAGG
                     VAVDICTDCGPGLAADKATLAEALRPTAWLQSDHPRLKAIARPIARLSTDEARKMALL
                     ADKARPYLKTIDFAG"
     gene            1220..3223
                     /locus_tag="VGB57_12830"
     CDS             1220..3223
                     /locus_tag="VGB57_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0132276.1"
                     /translation="MNRFFLALLLLCAFPAQAQLPPGQKAMSVHLAPETGRPAAGSKV
                     TVALVMSPRPGWHGYWQNPGDAGVGTRIAWRLPKGVAAGPIRYPVPERLIIQGLMNYV
                     FEGEHALLVDLQLPRRLAPGTKLPVRGKVDYLVCTDRICVPESAELSADLEVSAAPAP
                     PLPSFDRYRQALPRPLPEPGRFERRGAMLRLAVPLPASVEVEDPYFYPITEQALVYSA
                     PQAVSRRGNWLIVETQSGQGSPAALEGVLKLGPGQGVAVRATPGEVGPAGTPASPAAL
                     RDPPGYRTVLLALAGALLGGLLLNIMPCVFPILSLKALSLARAGGEEREARREALAYT
                     AGAVLACLALGAVLLAVRASGAAVGWAFQLQDPRVILFLLLLVTAIALNLAGLFELPS
                     LAGRLGGAGTGAFGTGALAAFVATPCTGPFMGAALGAALILPAAAALAVFAGLGLGLA
                     LPFLLLGYVPALRRRLPRPGPWMVRFRHILAVPMGLTALGLAWILERQTGLAGLAVGF
                     AAAAALGLILWWAGRRHGWLPLAPALLASGVAVAFLPAPSAPAEARTGGSLHAEPFSE
                     ARLAQLRAEGRPVFVYFTADWCLTCKVNEAAVLDRDEVAAAFRDKNAAVLVGDWTRGD
                     AAIGRFLEKQGRSGVPLYLWYAPGREAEVLPQILTVGRVRGLS"
     gene            complement(3317..4972)
                     /locus_tag="VGB57_12835"
     CDS             complement(3317..4972)
                     /locus_tag="VGB57_12835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0132277.1"
                     /translation="MIRKLLAALAALCMTSAATSPALADVLVDNVNGYTIGADGRLIR
                     FGAMLVGDDGKVERLYQRGEKTQRTRFRFDGRGRTLLPGLIDAHGHVMGLGLNALQLD
                     LSDTRSLQEAQARIAAYAAANPTPRWIIGRGWNQETWGLGRFPTAAELDAVVRDRPVW
                     LERVDGHAGWANSAAMKEAGITASTKSPAGGQIEMARGQPTGIFVDAASSLIDKVVPA
                     ALPRTRDQALDRAQEIMLSNGLTAAADMGTSPDDWLVMRRAGDDGRLKVRIIAYGAGV
                     ESALAIAGTRPTPWLYGDRLRLVGVKLYSDGALGSRGAWLKQPYKDSPGTRGLSFVTE
                     TELLNIMSRAAMDRFQVAVHAIGDAANAQALAAFEEMAVTYKGDRRWRIEHAQIVDPA
                     DLARFGRNGIIASMQPVHETSDWRMAEARMGLERLGGAYAWRSMIANRSPLAFGSDFP
                     VESPNPFPGLAAAISRQDPSGQPPGGWLPEQRVTLEQALAAFTRGAAYAGFAEDRIGS
                     LEKGRWADFILIDRDIFDVPTPRQLRETRVVETWIAGKKVWPN"
     gene            5028..6248
                     /locus_tag="VGB57_12840"
     CDS             5028..6248
                     /locus_tag="VGB57_12840"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY0132278.1"
                     /translation="MADLPVTLADIEAAARRIEGAVVRTPTLLSRTLSEATGARVWVK
                     FENLQFTAAYKERGALNKLLLMDPGTRSRGVIASSAGNHAQAVAYHGRRLGVPVTIVM
                     PVSTPSIKVKQTEGHGARIVLHGEMYDEAYGEALRIAEAESLTFVHPFDDSEIMAGQG
                     TVALELLADAPEIDTLVVPIGGGGLISGVATAAKALRPDLAIVGVQAELYPSMYCRIS
                     GKEMPSSGDTLAEGIAVKKPGRLAPPIIERLVDEILLVHEREIEIAVSLYLQIEKTVA
                     EGAGAAGLAALLAHPDRFRDRNVGLILTGGNIDTRLLATVLLRDLARSGRMARLRIQL
                     QDRPGALLAVMKLFDAHGANIIEIYHQRVFTTLPAKDAFIDVECEARDAEHLEALVRA
                     LEEAGFYVHPVEIR"
     gene            6374..7132
                     /locus_tag="VGB57_12845"
     CDS             6374..7132
                     /locus_tag="VGB57_12845"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY0132279.1"
                     /translation="MSAPFRFPRFFVTSPAPCPYLAGKTERKVFTELAGQHAADLNDA
                     LGRIGFRRSQAVAYRPSCLDCSACVSVRVVAAEFQPSASQRRMLKRHSELEVTACKPW
                     ATEEQYALLRRYLKRRHPDGGMVGMDESDFSDMIEQSPVKTYVIEYREPSVGGRPGKL
                     VGACLTDQQADGLSMIYSFFEPDDPARPGLGTYIILDHIVRAGKAGLPYVYLGYWVES
                     SRRMAYKARFRPLERLGPGGWNRFDPDQRELPLR"
     gene            7260..>7504
                     /locus_tag="VGB57_12850"
     CDS             7260..>7504
                     /locus_tag="VGB57_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132280.1"
                     /translation="MRRLVDAGEVESVGGRILLEERFRALQRQVPLLYIVIFANMVGL
                     YLASGGRFDSLFDFPLLVGALIVMRVAHWLKVRNRILP"
BASE COUNT         1062 a         2653 c         2578 g         1211 t
ORIGIN      
        1 ccggcaaagt ctatcgtctt cagatagggc cgcgccttgt ccgcgagcag cgccattttt
       61 cgcgcctcat cggtcgaaag ccgggcgatg ggccgggcga tcgccttcag gcgaggatgg
      121 tcgctctgca gccacgcggt cggcctgagc gcttcggcta gggtggcttt gtcggctgcg
      181 aggcccgggc cgcaatccgt gcagatatcg accgcgacgc ccccggcgga ggcgacggcg
      241 cgctgctcgc cggtttcggg caaggggaaa gccagactgt cccggtaggc gaaacggtag
      301 cggatccggc ctcgcatcgc ctcgggcgag atccggaagg gcgacttcac catggcgctc
      361 tgcaggaggc gataggggga atggggcttc agcgccgtct cccgatcgga aagctcgatg
      421 gtgacgctgg tccccatcat cggctgggtg gtcgagcgga ctcggccgtc cggcgccagc
      481 gtcagccggc cgaccgagcg cagctcgtgc ccttcgaagc gacggcggat cacggagaag
      541 ctgccgtcgg cattcgccgt cgccggatcc ggctcgatca cgacatgctc gaccgccgtc
      601 gccgcaacgg cgagattgtc gaactcgagg cgcggcgcct taatccggtt ccagccgcgc
      661 agcaggcccg atcccgagtc gaaccggacg tccggcggga gcggcaccgt ggcaacggtc
      721 cggcccgcag ccgtcgagcg gacgatctcc gcctggtcgg gcccgatccg tgcttcaacc
      781 cgagaccagc tccccccgat ccgggaatat tcgctgatcg acaccgggcg gccggaagga
      841 tcctccctga cgatccttcg ctcggagatg cgggtgatgc cttcgccgct gtcgacgagc
      901 atctgctgct ccgagacggt ctcccgtccg cccgcggcct ggcgggcttc gcttcggaca
      961 tgaccgatcg cagtgccgtc ggcggcgacg atccggtacc aggtttcagt ccggcgaggc
     1021 ggcgcgtccg cgcctgtcag cagcaaggcg caagcgagcc atagagccgg gccgagcggc
     1081 ggcacccgag gcggaacacg atggcgcgac gctaagtcca tgacacggca ggctagtggt
     1141 tgaataccgg cgtgggaagc acctcatcct ccccacgggc ttcgctcgca gggaggataa
     1201 tgaatgctat agccatccgg tgaatcgctt tttcctcgcc ctgctcctgc tctgcgcctt
     1261 ccccgcgcaa gcccagctgc cgcctgggca gaaggcgatg tccgtccatc tcgcccccga
     1321 aaccgggcgg ccggccgcgg gctcgaaagt cacggtggct ttggtcatga gcccgcgccc
     1381 gggctggcac ggctattggc agaatcccgg cgatgccggc gtcggcacgc ggatcgcctg
     1441 gcggcttccc aagggcgtgg cggccgggcc gatccgctat ccggtgccgg agcggctgat
     1501 catccagggg ctgatgaact atgtcttcga aggagagcat gccctcctgg tcgacctcca
     1561 gctgccgcgc cgactggcgc cggggacgaa gctgccggtg cgcggcaaag tcgattatct
     1621 cgtctgcacc gaccggatct gcgttccgga atcggccgag ctttccgccg atctcgaagt
     1681 gagcgccgct ccggcaccgc cgctgccgag cttcgaccgc taccgtcagg ccctgccgag
     1741 accgctacct gaacccggcc ggttcgagcg gcgaggcgcc atgctccggc tcgccgtccc
     1801 gctgcccgct tcggtcgagg tcgaggaccc gtatttctac ccgatcaccg agcaggctct
     1861 ggtctattcc gcgccgcagg cggtgtcgcg gcgcggcaac tggctgatcg tcgagaccca
     1921 gtcggggcaa ggcagccccg ccgcgctcga aggcgtgctc aagctcggcc ccggccaggg
     1981 ggtggcggtg cgcgcgacgc ccggcgaggt cgggcccgcc ggaactccgg cgagtcccgc
     2041 tgcgctacgg gacccgccgg gctaccggac cgtccttctc gccctcgccg gcgccctgct
     2101 cggcgggctt ctgctcaaca tcatgccctg cgtcttcccc atcctcagcc tcaaggcgct
     2161 gagcctcgcc cgcgccggcg gcgaggagcg cgaggcgcgg cgcgaggcgc tcgcctacac
     2221 cgccggcgcc gttctcgcct gcctcgcttt gggggcggtg ctgctggccg tgcgggcgag
     2281 cggggcggcg gtcggctggg cgttccagct ccaggacccg cgggtcatcc tcttcctgct
     2341 cctcctcgtc accgccatcg cgctcaacct cgccggcctg ttcgagctgc cctcgctcgc
     2401 cgggcggctc ggcggcgccg ggacgggcgc cttcgggacc ggggcgctgg ccgccttcgt
     2461 cgcgaccccg tgcaccggtc ccttcatggg agcggccctg ggcgcggccc tgatcctgcc
     2521 cgcggcggcg gcgctggccg tcttcgcggg tctcggcctc ggcctcgctc tgcccttcct
     2581 cttgctcggc tacgtccccg ccttgcggcg aaggctgccg aggcccggcc cgtggatggt
     2641 ccgcttccgc cacatcctgg cggtgccgat gggcctgacc gcgctcgggc tcgcctggat
     2701 cctcgagcgg cagaccgggt tggcgggact ggcggtgggg ttcgccgcgg cggccgcgct
     2761 cggcctcatc ctgtggtggg cggggcgccg gcacggctgg ctgccgcttg cccccgcctt
     2821 gctggcgtcg ggcgtggcgg tagccttcct cccggctccg tcggccccgg ccgaagcccg
     2881 caccggcggc agcctccacg ccgagccgtt cagcgaggcg aggctggcgc agctgcgcgc
     2941 cgaggggcgg ccggtcttcg tctatttcac cgccgactgg tgcctcacct gcaaggtcaa
     3001 cgaggccgcg gtgctcgacc gggacgaagt cgccgccgct ttccgcgaca agaacgcggc
     3061 cgtactggtc ggcgactgga cccggggcga cgcggcgatc gggcggttcc tggagaagca
     3121 gggacggtcg ggcgtgccgc tctatttgtg gtacgcgccc gggcgggagg cggaggtgct
     3181 gccgcagata ctgacggtgg gacgggtgcg ggggctgagc tgacatccgt catcccggcg
     3241 aaagccggga tctcaggacg agaagtctcg gcgggtctcc ccgaggtccc ggcttccgcc
     3301 gggatgacgg gacatcctaa ttgggccaga ccttcttccc cgcgatccag gtctccacca
     3361 ccctggtctc gcgcagctgg cggggggtgg ggacgtcgaa tatgtcgcgg tcgatcagga
     3421 tgaaatcggc ccagcggcct ttctcaaggc ttccgatccg gtcctcggcg aagccggcat
     3481 aagccgcgcc ccgggtgaag gcggcgagcg cctgctcgag ggtcacgcgc tgctcgggga
     3541 gccagccgcc ggggggctgg ccgctcgggt cctggcggct gatcgcggcg gcgaggccgg
     3601 ggaaggggtt gggggattcg accggaaagt cggagccgaa ggcgagcggc gagcggttgg
     3661 cgatcatgct ccgccaggca taagcgccac ccaggcgctc cagccccatc ctcgcctccg
     3721 ccatccgcca gtcgctggtc tcgtggaccg gctgcatcga ggcgatgatt ccgttgcggc
     3781 cgaagcgggc gaggtcggcg ggatcgacga tctgggcatg ctcgatccgc cagcgccggt
     3841 cgcccttgta ggtgacggcc atctcctcga acgccgccag cgcctgggcg ttggcggcgt
     3901 cgccgatggc gtggacggcg acctggaagc ggtccatcgc cgcccggctc atgatgttga
     3961 gcaattcggt ctcggtgacg aagctcaggc cgcgggtgcc ggggctgtcc ttgtagggct
     4021 gcttgagcca ggctccgcgc gagcccagcg ctccgtccga gtagagcttg accccgacca
     4081 gccggagccg gtcgccgtag agccagggcg tcggccgggt gccggcgatg gcgagtgcgc
     4141 tctcgacccc ggcgccgtag gcgatgatcc tgaccttgag ccgcccgtcg tcgccggcgc
     4201 ggcgcatgac cagccagtcg tccgggctgg tgcccatgtc ggcggcggcg gtgagtccgt
     4261 tggagagcat gatctcctgc gcccggtcca gcgcctggtc gcgggttcgg ggaagggccg
     4321 ccggaaccac cttgtcgatg agcgaggagg cggcgtcgac gaagatcccg gtgggctgcc
     4381 cgcgcgccat ctcgatctgg ccgccggcgg gggatttggt cgaggcggtg atccccgctt
     4441 ccttcatcgc cgcgctgttc gcccagccgg catggccgtc cacccgctcc agccacaccg
     4501 gcctgtctct gacgacggca tcgagctcgg cggcggtcgg gaagcggccg aggccccagg
     4561 tttcctggtt ccagccgcgg ccgatgatcc agcgcggcgt cggattggcg gcggcatatg
     4621 cggcgatcct ggcctgcgcc tcctgaagcg acctggtgtc ggaaaggtcg agctgaagcg
     4681 cgttgaggcc gagccccatg acatggccgt gcgcatcgat caggccgggc agcaaggtcc
     4741 gcccccggcc gtcgaagcgg aagcgggtcc gctgggtctt ctcgccgcgc tggtagagcc
     4801 gctcgacctt gccgtcgtcg ccgaccagca tcgccccgaa gcggatcagc cggccgtcgg
     4861 cgccgatcgt atagccgttg acgttgtcga cgaggacgtc ggccaaggcg ggggatgtgg
     4921 ccgccgaggt catgcacagg gcggccagcg ccgccagaag ctttctgatc atcgtagagg
     4981 cgataagcgt tgtttcgcgg cggcgccaga tgctctaacg acccgccatg gccgacctcc
     5041 cagtcaccct cgccgacatc gaagcggcgg cgcggcggat cgaaggcgcg gtggtgcgca
     5101 cccccaccct gctcagccga accctctcgg aggcgacagg ggccagggtc tgggtgaagt
     5161 tcgagaacct ccagttcacc gccgcctaca aggagcgcgg cgccctcaac aaattgcttc
     5221 tgatggaccc cgggacccgc tcgcgcggag tgatcgcctc ctctgccgga aaccacgccc
     5281 aggcggtcgc ctatcacgga cgccggctcg gcgtcccggt caccatcgtg atgccggtct
     5341 cgaccccgag catcaaggtc aagcagacgg aaggccatgg cgcccgcatc gtccttcacg
     5401 gcgagatgta cgacgaggcc tatggcgagg cgctccgaat cgccgaggcg gagagcctga
     5461 ccttcgtcca tccgttcgac gattcagaga tcatggccgg gcagggcacg gtggcgctcg
     5521 agctgctcgc cgacgctccg gagatcgaca cgctcgtcgt tccgatcggc ggcggcggtc
     5581 tcatctcggg agtcgccacc gcggccaagg ccttgcgacc cgacctcgcg atcgtcggag
     5641 tccaggccga gctctacccg agcatgtact gccggatcag cggcaaggag atgccttcct
     5701 ccggcgacac gctcgccgag ggcatcgcgg tcaagaagcc gggccggctc gccccgccga
     5761 tcatcgagcg cctcgtcgac gagatattgc tggtccacga acgcgagatc gagatcgcgg
     5821 tcagcctcta cctccagatc gagaagacgg tggccgaagg ggcgggggcg gccgggctcg
     5881 ccgcccttct cgcccatccc gaccgctttc gcgaccgcaa cgtcgggctg atcctcaccg
     5941 gcggcaatat cgacacgagg ctgctcgcca ccgtcctctt gagggacctc gcccgctccg
     6001 gccggatggc gcggctccgg atccagctcc aggaccgacc gggcgccttg ctcgcggtga
     6061 tgaaattgtt cgacgctcac ggcgccaaca tcatcgagat ctaccaccaa agggtcttca
     6121 ccactctccc ggccaaggac gccttcatcg acgtcgagtg cgaggcgcgc gatgcggagc
     6181 atctcgaggc cctcgtccga gcgctcgagg aggccggctt ctacgttcat cccgttgaga
     6241 ttcgctgagc cgccgtcccg cataggaacg tatgatggtt aacgccgttt cggcttctcc
     6301 agacccttcc caagcggtcg gcaaacacgc ataaaccgag tctcaaccaa gccttttaaa
     6361 aaccggaacc ccgttgagcg cacccttccg ctttccccgc ttcttcgtca ccagcccggc
     6421 accctgcccg tatctggccg gaaaaaccga gcgcaaggtg ttcaccgagc ttgccgggca
     6481 gcacgcggcc gacctgaacg acgcgctcgg ccggatcggc ttccgtcgga gccaggccgt
     6541 cgcctaccgg ccctcctgcc tcgattgctc ggcctgcgtc tcggtccggg tcgtcgccgc
     6601 cgagttccag ccgagcgcca gccagcgccg gatgctgaag cgccattccg agctcgaggt
     6661 caccgcctgc aagccctggg cgaccgagga gcaatatgcc cttctccgcc gctacctgaa
     6721 gcggcgccac ccggacggcg gaatggtcgg gatggacgag agcgacttct cggacatgat
     6781 cgagcaatcg ccggtcaaga cctacgtcat cgaatatcgc gagccttccg tgggcggccg
     6841 ccccggcaag ctcgtcggcg cctgcctcac cgaccagcag gccgacgggc tttcgatgat
     6901 ctacagcttc ttcgagccgg acgatccggc gcggcccggc ctcggcacct acatcatcct
     6961 cgaccatatc gtccgcgccg ggaaagcggg gctcccctac gtctatctcg gctattgggt
     7021 cgagagctcg cggcggatgg cctacaaggc gcgcttccgc ccgctcgagc ggctcggccc
     7081 cggcggctgg aaccgcttcg acccagacca gcgcgagctt cctttgaggt gatgctcccc
     7141 ggcgaaggcc ggggcccatg ccctttccgc ccgcgccgct ggaccccggc cttcgccggg
     7201 gaaccacctg ttaatcgttt ccgcgtaaat cgcggtccat ggggcgggga atttcgaaat
     7261 tgcgccggct cgtggatgcc ggcgaggtgg agagcgtagg ggggcgaatc ctcctcgagg
     7321 agcgcttccg ggcgctgcag cgccaggtcc cgcttctcta catcgtcatc ttcgccaaca
     7381 tggtgggcct ttacctggcc tccggggggc ggttcgactc tctcttcgac ttcccgctgc
     7441 tggtcggcgc tctgatcgtc atgagggtcg cccattggct caaggtccgc aaccggatcc
     7501 ttcc
//
LOCUS       DASWNU010000639         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_24244, whole genome shotgun sequence.
ACCESSION   DASWNU010000639 DASWNU010000000
VERSION     DASWNU010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24244"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /locus_tag="VGB57_12855"
     CDS             complement(<1..1954)
                     /locus_tag="VGB57_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEY0132281.1"
                     /translation="MFSKILIANRGEIACRVIRTARKMGIATVAVYSDADARAPHVKM
                     ADESVRLGPPPASESYLNADLIIEACRATGAEAVHPGYGFLSERESFARALEEAGIAF
                     IGPPPGAIAAMGDKIESKKLALEAGVSVVPGFVGEIGDTEHAVRIAGEIGYPVMMKAS
                     AGGGGKGMRLAYSERDVREGFEATKREGLASFGDDRVFIEKFVENPRHIEIQLIGDKA
                     GNLLYLGERECSVQRRHQKVVEEAPSPFVTPEMRKAMGEQAVALARAVGYFSAGTVEF
                     IAGADRSFYFLEMNTRLQVEHPVTEEVTGLDLVELMIRVAAGEDLPFGQDEVKLNGWA
                     IENRVYAEDPYRGFLPSTGRLVRYRTPAASSEGGVTRIDDGVEEGGEVSMFYDPMIAK
                     LITWAPTREAAIDRQADALDSFVIEGIGHNVDFLSALMQHPRFREGRLTTGFIAEEYP
                     EGFEGAPADDVLIEDLAAIGAMLAMETEAHACSVSGQLGAPVTPPEERVVRLGGRSFA
                     IRFDGFEGGLLAHLDDREGREMIGRWEPGQRLFKGVIDGRSRIVQVARRGRQWRLTTR
                     GASHLVEVLPAHVAELSRFMIEKVPPDLSRYLLCPMPGLLTALNVGEGDKVEAGQPLA
                     VVEAMKMENILRAEKTGVVKSVSARPG"
     gene            complement(2078..2428)
                     /locus_tag="VGB57_12860"
     CDS             complement(2078..2428)
                     /locus_tag="VGB57_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132282.1"
                     /translation="MIDEQEGPPRGDHDGDHYLYDFFKYLTSVSLLSLGALFTFLGMD
                     TAKGVPEVAKVAAVGVLGLAAFMAFFGAEQLVAAKAKGERIGPAVYRLRKWAPQVYLV
                     GFAILAYICGDILR"
BASE COUNT          413 a          902 c          797 g          413 t
ORIGIN      
        1 cgccggggcg ggccgatacc gacttcacga cccccgtttt ctccgccctg aggatattct
       61 ccatcttcat ggcctcgacg acggcgagcg gctgccccgc ctcgaccttg tccccttcgc
      121 ccacgttcaa agcggtcagg aggccgggca tcgggcagag aaggtatctg gagaggtccg
      181 gcggcacctt ctcgatcatg aatcggctga gctcggcgac gtgggccggc aggacctcga
      241 cgagatggga ggcgccgcgg gtcgtcaggc gccactgccg gcctcgacgg gcgacctgga
      301 cgatccggct ccggccgtcg atcacaccct tgaacaggcg ctggcccggc tcccaccgcc
      361 cgatcatctc gcgcccctcg cggtcatcga gatgggcgag cagcccgcct tcgaagccgt
      421 cgaaccttat cgcgaagctc cggccgccga gcctgaccac ccgctcctcg ggcggcgtca
      481 ccggggctcc gagctggccg ctcaccgagc aggcatgagc ctcggtctcc attgccagca
      541 tggcgccgat cgccgccagg tcctcgatca ggacatcgtc cgccggagcg ccctcgaacc
      601 cttcgggata ttcctcggcg atgaagccgg tggtgagccg cccctcccgg aagcggggat
      661 gctgcatcag cgcggagagg aagtcgacat tgtggccgat cccctcgatc acgaaggaat
      721 cgagcgcgtc ggcctgtcgg tcgatcgccg cctcccgggt cggcgcccag gtgatcagct
      781 tggcgatcat cggatcgtag aacatcgaga cctcgccgcc ctcctcgacc ccgtcgtcga
      841 tccgggtgac gcccccttcc gacgaagcgg cgggggtccg gtagcggacg agccgcccgg
      901 tcgagggcag gaagccgcga taggggtcct cggcatagac ccggttctcg atcgcccagc
      961 cgttgagctt cacttcatcc tggccgaacg gcaggtcctc gcccgcggcg acccggatca
     1021 tcagctcgac gaggtcgagg ccggtcacct cctcggtcac cggatgctcg acctggagcc
     1081 gtgtgttcat ctcgaggaag tagaagcttc ggtcggctcc ggcgatgaac tcgacggtgc
     1141 cggcgctgaa atagccgacg gcgcgcgcca gcgcgaccgc ctgctcgccc atcgccttgc
     1201 gcatctcggg cgtgacgaag ggcgacggcg cttcctcgac caccttctgg tggcgccgct
     1261 ggaccgagca ttccctctcg ccgaggtaga ggaggttgcc ggccttgtcg ccgatcagct
     1321 ggatctcgat gtggcgcgga ttctcgacga acttctcgat gaacacccgg tcgtcgccga
     1381 agctcgccag cccctcgcgc ttggtcgcct cgaagccttc gcggacgtcc ctctcggaat
     1441 aagcgagccg catgcccttg ccgccgccgc ccgccgaggc cttcatcatc accggatagc
     1501 cgatctcgcc ggcgatcctc accgcatgct cggtatcgcc tatctccccg acgaagcccg
     1561 gcaccacgct gacccccgcc tcgagcgcca gcttcttcga ctcgatcttg tcccccatcg
     1621 ccgcgatcgc ccccggcggc ggcccgatga acgcgatccc cgcttcctcc agcgcccggg
     1681 cgaagctctc gcgctcggac aggaagccgt agccgggatg cacggcctcg gcaccggtcg
     1741 cccggcaggc ttcgatgatc aggtcggcgt tgaggtagct ctcgctcgcc ggcggcgggc
     1801 cgagacggac cgactcgtcg gccatcttca catgcggcgc ccgggcatcg gcgtccgaat
     1861 agacggcgac ggtggcgatg cccatcttcc gggcggtccg gatgacccgg caggcgatct
     1921 cgccgcgatt ggcgatcagg attttagaga acatgcacct acctcgtcat gccggacttg
     1981 atccggcatc cacctgtatc tcgcggaccg gtcgtaaaga aggtggaccc cggatcaagt
     2041 ccggggtgac gaggtcgacc cggcttggcg gtgaaggtca acgaaggatg tccccgcaaa
     2101 tgtaggcgag tatcgcgaaa ccgacgagat agacttgcgg cgcccatttg cgcagccggt
     2161 agacggcggg cccgatccgc tcgcctttcg ccttggccgc gaccagctgc tccgccccga
     2221 aaaaggccat gaacgccgcc aggccgagaa ccccgacagc ggcgaccttg gcgacctccg
     2281 gcacgccctt cgccgtgtcc atgcccagga aggtgaacag ggcgcccagc gacagcagcg
     2341 acaccgacgt cagatatttg aagaaatcgt agagataatg gtcgccgtca tgatcgcccc
     2401 ggggcggtcc ctcctgctcg tcgatcattt ggcgcctttc tcgaacatca gcgatctgtg
     2461 cgtctggaca ggattataac ggcagtgacc agagcgacga acccgccgac tatcgccgcg
     2521 gcccg
//
LOCUS       DASWNU010000640         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_362473, whole genome shotgun sequence.
ACCESSION   DASWNU010000640 DASWNU010000000
VERSION     DASWNU010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362473"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGB57_12865"
     CDS             <1..318
                     /locus_tag="VGB57_12865"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0132283.1"
                     /translation="LKVENSQTAFTSRYETGEDIVIPVGHHDGNYQADPETLDRLEGE
                     GRIAFRYAEACNGSARDIAGIVSESGNVLGMMPHPERAAEPAHGRTDGRRLFEGLIGG
                     LGV"
     gene            complement(322..1194)
                     /locus_tag="VGB57_12870"
     CDS             complement(322..1194)
                     /locus_tag="VGB57_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY0132284.1"
                     /translation="MAPFRRAAPYVVHLRLPFQLTLAPIFLWGALLSGGRWDEATTAA
                     FVALHLFLYPAATAFNSAFDNDEGPVSGLERPPEVPPGLLGFAVVLAAAGAVIALFAG
                     LAFLFVYAPIVVWTAAYSHPSTRWKASPWKSAAAIALGQGALGFAAGWIAAAPLDLGD
                     EALFAGAAGAALTALGLYPATQVFQLEEDRERGDRTLALALGPAAALRFGAACLALGG
                     AITAWLVERRFGPPDALLIALGYSALVICLERLARRPAGPAETGLYRPAMRITRFATA
                     GFLLFLALQAVGSG"
     gene            complement(1348..>2987)
                     /locus_tag="VGB57_12875"
     CDS             complement(1348..>2987)
                     /locus_tag="VGB57_12875"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0132285.1"
                     /translation="DGGNGSDLISGGAGNDVIDGGNGQDTLVLDGKRSDYRVMEGLNG
                     AIVVRDLRANGQDGEDRLFSIERIQFSDGTFKLVDLIEANGAPVAQNDALTLAENAGK
                     TEITSVLLANDSDADGDSLIVSAVQAVSAQGAAVSIGPDGKVSYDAGSIFADLDNGET
                     ATDSFTYTITDGNGHVSTATATVTITGVGQNTAPVAVNDKMTLAEDAGPTDVTSNLIG
                     NDIDPDGDSFQVTSVQAVSNRGVVVTVGPDGKVTYDAGQIFMDLEAGEKLTDTFAYTI
                     TDSQGLTSTATASVTITGVTQEPDAYFYIQEDATRDDLLGLLTDWLDMGIVGVDSSKG
                     TLGTVSFEGGVLSFAADHDFSDHLLPDQSHSTTFTVTGPDGQKKTIEAIVEGVNDQIV
                     AVDDAFAVGEGQTSVNLWKAIVSNEIDPDGSIHSNRILEVGTEGTQGLVSFDASSRTL
                     TYSAANIDLAPGETMTDSFTYVVTDGWGSQDTATVFVTVTGAAGGGAMTSMSGGESIM
                     SAFVGGAGGSHLDDAEFASFPSMQAMQEMLVADAVIA"
BASE COUNT          472 a          992 c         1071 g          452 t
ORIGIN      
        1 ttgaaggtcg agaacagcca gaccgctttc accagccgct acgagacggg cgaggacatc
       61 gtcatcccgg tcggccacca tgacggcaat tatcaggccg atcccgaaac tctcgaccga
      121 ctcgagggtg aaggccggat cgccttccgc tacgccgagg cctgcaacgg ctcggcccgc
      181 gacatcgccg ggatcgtcag cgagtccggc aacgtgctgg gaatgatgcc gcaccccgag
      241 cgcgcggcgg aacccgccca cggccgcacc gacggccggc ggctgttcga gggcctgatc
      301 ggcgggctcg gcgtctgaga cctagccgct gccgacggcc tggagcgcga ggaagaggag
      361 gaacccggcc gtcgcgaagc gggtgatccg catcgccggg cgatagagcc cggtttccgc
      421 cggacccgcc ggccgtcgcg ccagccgttc caggcaaatg accagggcgg aatagccgag
      481 cgcgatcaac aaagcgtcgg gcggcccgaa gcggcgctcc accagccagg ccgtgatcgc
      541 gccgccaagc gccagacagg cggctccgaa acggagcgcc gccgccggtc ccagggcgag
      601 cgccagcgtg cgatcgccgc gctcccgatc ctcctcgagc tggaacacct gggtcgccgg
      661 gtagaggccg agcgccgtca atgccgcgcc cgccgcgccg gcgaacaggg cttcgtcgcc
      721 caggtcgagc ggcgccgccg cgatccagcc ggcggcgaag ccgagagccc cctgccccag
      781 cgcgatcgcc gccgcgcttt tccacgggct cgccttccac cgggtcgacg gatggctgta
      841 ggccgcggtc cagacgacga tcggcgcgta gacgaaaaga aaggcgagcc ccgcgaacag
      901 ggcgatgacg gcgccggcgg ccgccagcac gacggcgaag ccgagcagcc ccggcggcac
      961 ttccggaggc cgctccagcc cgctcaccgg cccctcgtca ttgtcgaagg ccgaattgaa
     1021 ggcggtagcc gcggggtaga gaaagaggtg gagagcgacg aaagcggcgg tcgtcgcctc
     1081 gtcccagcgc ccgcccgaca gcaaggcgcc ccagaggaag atcggcgcca gcgtcagctg
     1141 gaagggcagg cgcaggtgga cgacgtaggg cgcggcgcgg cggaacgggg ccatcgccgt
     1201 tcctacgcat ggcgcctccg gctgcgcaag gacgccgcgg cggatgattc cgaaccgtca
     1261 tcccggcttt ccccgggatg acgaaggcta agcgaaaggc cgcccccttt cgggagcggc
     1321 ctccgcttgt gccgatgggg cgggcgatca ggcgatgacc gcgtccgcca ccagcatctc
     1381 ctgcatggcc tgcatcgagg ggaagctggc gaattcggca tcgtcgagat gcgagccgcc
     1441 ggcgccgccg acgaacgccg acatgatcga ctcgccgcca gacatcgacg tcatggcgcc
     1501 gccgccggcc gcgccggtca ccgtgacgaa cacggtggcg gtgtcctgcg agccccagcc
     1561 gtcggtgacg acgtaggtga agctgtcggt catcgtctcg cccggcgcca ggtcgatgtt
     1621 cgcggccgag taggtcaggg tgcggctgga ggcatcgaac gacaccaggc cctgggtgcc
     1681 ctcggttccg acctcgagga tccggttgga gtggatgctt ccgtccgggt cgatctcgtt
     1741 ggagacgatc gccttccaca aattgacgct cgtctggccc tcgccgaccg cgaaagcgtc
     1801 gtcgaccgcg acgatctggt cgttgacgcc ctcgacgatc gcctcgatcg tcttcttctg
     1861 cccgtccggg ccagtgacgg tgaaggtcgt cgagtggctc tggtcgggca gcagatggtc
     1921 gctgaagtcg tggtcggccg cgaaggacag cacgccgccc tcgaacgaga cggtgccgag
     1981 cgtgcccttg ctcgagtcga ccccgacgat tcccatgtcg agccagtccg tcagcagccc
     2041 gagcagatcg tcgcgggtgg cgtcttcctg gatatagaaa taggcgtccg gctcctgggt
     2101 cacgccggtg atcgtcaccg atgccgtcgc cgtggaggtg aggccctggg agtcggtgat
     2161 ggtgtaggcg aaggtgtcgg taagcttctc gccggcctca agatccatga agatctggcc
     2221 ggcgtcgtac gtcaccttgc cgtcggggcc gacggtgacc acgacgcccc tgttggagac
     2281 ggcctggacg gaggtgacct ggaaggagtc gccgtccgga tcgatgtcat tgccgatcag
     2341 gttcgaggtg acgtcggtcg ggccggcatc ctcggcgagg gtcatcttgt cgttgacggc
     2401 gaccggagcg gtgttctggc cgaccccggt gatcgtcacc gtcgcggtgg cggtcgagac
     2461 gtggccgttg ccgtcggtga tcgtgtaggt gaagctgtcg gtcgcggtct cgccgttgtc
     2521 gaggtcggcg aagatcgaac cggcgtcgta gctgaccttg ccgtccgggc cgatgctgac
     2581 ggccgcgccc tgggcggata cggcctggac ggcgctgacg atcagggaat cgccgtcggc
     2641 gtcgctgtcg ttggcgagga gcaccgaggt gatctcggtc ttgccggcgt tttcggcgag
     2701 ggtcaaagcg tcgttctggg cgacgggcgc cccattggcc tcgatgaggt cgacgagctt
     2761 gaaggttccg tcggaaaact ggatccgctc gatgctgaac agccggtcct cgccgtcctg
     2821 gccgttggcg cgaaggtcgc ggacgacgat cgcgccgttc aggccttcca tgacccggta
     2881 gtcggagcgc ttgccgtcga ggacgagcgt gtcctggccg ttgccgccgt cgatgacgtc
     2941 gttgccggcg ccgccggaga tcaggtcgct gccgttgccg ccgtcga
//
LOCUS       DASWNU010000641         3496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_465441, whole genome shotgun sequence.
ACCESSION   DASWNU010000641 DASWNU010000000
VERSION     DASWNU010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465441"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..510)
                     /locus_tag="VGB57_12880"
     CDS             complement(61..510)
                     /locus_tag="VGB57_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132286.1"
                     /translation="MSRALLVLALATTGCAHAPPAVPCEAANLPFAARALPDILALAD
                     SPPATLEAGFVLCSRHRDRLLAASAALGAEGYSSYIGASDRGQCLRVARTHEAGVPAL
                     EREMAAMCRIAGAHRIAYRRWQTEVGGKSVRLAGGRLTIDGKARQVD"
     gene            complement(507..2345)
                     /locus_tag="VGB57_12885"
     CDS             complement(507..2345)
                     /locus_tag="VGB57_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132287.1"
                     /translation="MKFMNLPANLPNRRRGSRIGSLPAFLLLLATLLAAPATAQAPAP
                     REWTPAQVQAILDKTETIRLAPTLAHLSPGERVAVTKLLEVGRIFQDVYEQQRHRNAL
                     TVRAALARRTDPQGRALYTLYRLNQGPIATTLENQREPALAVPNAPPGKNVYPWDLTK
                     AELDAYLAAHPDERARLTDLRSVVRRAEMAVLKRDLAKLRQYPVIDALHPGLRARIEA
                     LARRPARATLYAVPYSLAYADEMVRSHGLLNEAADAVAGSDPQFARYLRNRARDLISD
                     DYESGDAAWLKGRFKNLNAQIGAYETYDDELLGTRAFYGLSLLAIRTKESAELREAMK
                     GIQELENSLPYERRRRVMEDIPVGVYDVIADFGQTRGGNTATNLPNEAYLVERYGSII
                     LLRANIMRNPDLFRGSGDIFGAAIVPAQAGHLTADANFYRTLWHEVGHYLGPDVTASG
                     QALDVIGADSNLLEELKADLVSLFVAKELRRRGYYSEDQLRAVYASGILRTLQNNKPR
                     REQPYNMMQLMQWNWFLDRRVLSFDPATAKLGIDYGRYHEAVRDLLKQVIAIQEAGDA
                     AAASAFVDRWGKWDDALHGRVAANIRAQQRYRFRLFEYSALGAARR"
     gene            2826..3245
                     /locus_tag="VGB57_12890"
     CDS             2826..3245
                     /locus_tag="VGB57_12890"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HEY0132288.1"
                     /translation="MEELLIGLTRGNVPEVKIVLATVVALLAAYQVGLMAVGYGKVRL
                     PFLKPLPASKAHRAIGDSIVAVTVTVAIMCIGYFGFEDDAAVHILLACALLAVLGFKI
                     LVVRRLHKLGHLLPALGIAVFLLFWATWLSAAAGHLF"
     gene            3257..>3496
                     /locus_tag="VGB57_12895"
     CDS             3257..>3496
                     /locus_tag="VGB57_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132289.1"
                     /translation="MEDGVEHHGAKGGGTGLQLLGSLAAAAVIVALAIWAVTAKLGPT
                     GIAGREAAEERAEAAEEAGEEAGEARGESGRNRRRG"
BASE COUNT          545 a         1085 c         1289 g          577 t
ORIGIN      
        1 gaagaacccc tccaccatgc ttcgcatggt ccccctcccc cggaaagccg ggggaggatt
       61 tcagtcgacc tgccgcgcct tcccgtcgat cgtcagacgg ccgccggcga gccggacgga
      121 cttgccgccc acctcggtct gccagcggcg ataggcgatc cggtgggccc cggcgatccg
      181 gcacatcgcc gccatctccc gttcgagggc cgggacgccg gcctcgtggg tccgggcgac
      241 gcgcaggcac tgtcccctgt ccgaggcgcc gatgtagctc gaatagccct ccgctcccag
      301 tgccgcggag gccgcgagaa ggcggtcgcg atggcggctg caaaggacga agccggcttc
      361 cagggtcgcc ggcggcgagt ccgccaaagc cagtatgtcg ggcaacgccc gagcggcgaa
      421 gggcaggttg gccgcctcgc aaggaacggc ggggggcgcg tgagcgcagc ccgtcgtcgc
      481 gagggccagg acaagcaggg cccgactcac ctccgcgccg cccccagcgc cgaatattcg
      541 aacaggcgga agcggtagcg ctgctgggcg cggatgttgg cggccacgcg tccgtggagc
      601 gcgtcgtccc actttcccca gcgatcgacg aaggcgctcg ccgcggcggc gtcgcccgcc
      661 tcctggatgg cgatgacctg cttcaagagg tcgcgcaccg cctcgtgata gcggccgtaa
      721 tcgatgccga gcttcgccgt cgcgggatcg aagctcagca ccctgcggtc gaggaaccag
      781 ttccactgca tcagctgcat catgttgtag ggctgctcgc ggcgcggctt gttgttctgg
      841 agggtgcgca ggatgccgct ggcgtagacg gctcgaagct ggtcctcgct gtaatagccg
      901 cggcggcgaa gctccttcgc gacgaacagg gagacgaggt ccgccttgag ctcctcaagc
      961 agattggagt cggcgccaat gacgtcgaga gcctggcccg acgcggtgac gtcggggccg
     1021 agataatggc cgacctcgtg ccacagcgtt cggtagaaat tggcatcggc ggtgaggtgg
     1081 ccggcctggg cggggacgat cgcggcgccg aaaatgtcgc ccgagccgcg gaagaggtcc
     1141 ggattgcgca tgatgttggc tcgaagcagg atgatgctcc cgtagcgctc gaccagatag
     1201 gcttcgttgg gaaggttggt cgcggtgttg ccgccgcggg tctggccgaa atcggcgatg
     1261 acgtcgtaga ccccgaccgg aatgtcctcc atcacccgcc ggcgccgctc gtagggcagc
     1321 gagttctcga gctcctggat gcccttcatc gcctcgcgaa gctcggccga ttccttcgtt
     1381 cggatagcga gcaggctaag cccgtaaaag gcgcgggtgc cgagaagctc gtcgtcatag
     1441 gtctcgtagg cgccgatctg ggcgttgagg ttcttgaagc gccccttgag ccaggcggcg
     1501 tcgcccgatt cgtagtcgtc gctgatgagg tcgcgggcgc ggttgcgaag gtagcgggcg
     1561 aattgcgggt ccgacccggc gaccgcgtcg gccgcctcgt tgagcaggcc gtggcttcgg
     1621 accatctcgt cggcataagc gagcgaataa ggcacggcgt agagggtcgc tcgggcgggc
     1681 cttctggcga gcgcctcgat ccgggcgcgc agaccggggt ggagggcgtc gatgaccgga
     1741 tattggcgaa gcttggcgag gtcgcgcttg agcacggcca tctccgccct tcggacgacc
     1801 gagcgaaggt cggtgagccg ggcgcgctcg tccgggtggg cggcgagata ggcgtcgagc
     1861 tccgccttgg tgaggtccca gggatagacg ttcttgcccg gcggcgcgtt cgggacggcg
     1921 agcgccggct cgcgctggtt ctccagggtc gtggcgatcg gcccctggtt gaggcggtag
     1981 agggtgtaga gggcgcggcc ctgcggatcg gtgcgccgcg ccagggcggc ccggacggtg
     2041 agcgcgttgc ggtggcgctg ctgctcgtag acgtcctgga agatccgacc gacctcgagc
     2101 agcttggtca ccgcgacccg ctcgcccggc gagagatggg cgagcgtggg cgcgagccgg
     2161 atcgtctcgg tcttgtcgag gatcgcctgc acctgcgccg gcgtccattc gcgcggggcg
     2221 ggagcctggg cggtggccgg ggccgccagc aaggtggcga ggagcagcag gaaggccggc
     2281 agggaaccga ttcgcgagcc gcgcctccgg ttcggaaggt tcgccggaag gttcataaat
     2341 ttcataagtt cggcttccca agagcgaaaa acattcctga acctttcgaa gcttgaggcg
     2401 gccgcccggg ccctcggcat tacatcaaat ccagctgaaa cgcgcataat ccagactctc
     2461 aatcgtctgc tggagtataa caggaaaatt cctatttgtg aaatcctgtt tgaggttgct
     2521 gcgccgaaaa cgggttagcc gcccctgccc tcgccgtcat gctgaacttg tatcagcatc
     2581 cagggtggag ccgtgccgcg gtatgtcgtc agtgcttcga gcgcgtttcc tcttcggccg
     2641 gcaggccccg gatctttgga gagacggcgc aacggcgcaa cggcgcgacg tagagaagag
     2701 gctctctacc tcgcgtcgtt gcatcgacgc tcgccccaca tccagcgtcc ggacaggcgg
     2761 cgagctggac cctgaaacga gttcagggtg acggtggggt ggacaggcac taggctgtat
     2821 caggaatgga agagcttctc atcggcctga cccgcggcaa cgtgccggag gtgaagatcg
     2881 tgctggccac ggtcgtcgcc ttgctcgccg cctatcaggt ggggctgatg gcggtcggct
     2941 acggcaaggt ccggctgccg ttcctgaagc cgctgccggc gtcgaaggcg caccgggcga
     3001 tcggagattc gatcgtcgcg gtcaccgtca ccgtcgcaat catgtgcatc ggctatttcg
     3061 ggttcgagga cgacgcggcg gtccatatcc tgctcgcctg cgcattgctg gccgtgctcg
     3121 gcttcaagat cctggtggtg cgccggctgc acaagctcgg ccatctcctg ccggcgctcg
     3181 ggatcgcggt cttcctgctt ttctgggcga cctggctctc ggcggcggcc gggcatctgt
     3241 tctgaaggag tcgcgcgtgg aggacggcgt ggagcatcac ggggcgaaag ggggcgggac
     3301 ggggctccaa ctgctgggat cgctggccgc ggcggccgtg atcgtcgcgc tcgccatctg
     3361 ggcggtgacg gcgaagctcg gcccgaccgg catcgccggg cgagaggcgg cggaggagcg
     3421 ggccgaggcg gcggaagagg ccggggaaga ggccggggag gcgcgcgggg aaagcggcag
     3481 gaaccggcgc cgcggg
//
LOCUS       DASWNU010000642         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_465480, whole genome shotgun sequence.
ACCESSION   DASWNU010000642 DASWNU010000000
VERSION     DASWNU010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465480"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1028)
                     /locus_tag="VGB57_12900"
     CDS             complement(27..1028)
                     /locus_tag="VGB57_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase"
                     /protein_id="HEY0132290.1"
                     /translation="MSNIRPLTIPTEARERLRIMFIAKHALWEGGLHPEDGNHALYHV
                     EVREILKSLGLNLELANKYEALFEKPEVDFVFPLLNRGGFLNSEMMLPLLCTRHSIPF
                     LGASPILRGMSDDKHLTKMAVAARGIPTAPWAIYRRGAPVERARCPEGERMVIKPNAS
                     SASWGVQDAHDWAGVEAAIASIHSEGHDAIVEPFLNGSDVEVPVITIRGEPAIMPMML
                     FEQADPSHLRTYYEKRDLVDRSQKYQLVEFDDVEWMPVIADYTRRIAEEFRPFDYGRY
                     EFRFDRESGKVVFLEVNLNANLWSQKVYGRSAALAGLSQEELIETILAESLRRHGLL"
     gene            1103..>2014
                     /locus_tag="VGB57_12905"
     CDS             1103..>2014
                     /locus_tag="VGB57_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132291.1"
                     /translation="MTSPTPAPGDPAALMTTGGDARIVLKPETGLNRYHSAPRPSDVL
                     AYASSTANDISTPAFARVEALLAGLGPDLSGRAYAARLEALRVRIRSAYALAGEVDIV
                     FAPSGTDLEYVALACAAGRGRTGIHNILLGADEVGSGCVHSAFGRYFARETALGVATS
                     PGEPVPGLGETHVEMIDVPVRDGHGRARPSAEVAGRMDVAIAEAEAAGRHVLVHVVHG
                     SKTGLILPSLGDIDRLAESHGKAVTPVVDACQARITSPAIADYLERGAIVFVTGSKFM
                     GGPPFSGFALVPRRFREESDPIAHGLAT"
BASE COUNT          305 a          723 c          645 g          341 t
ORIGIN      
        1 agaaatctcg gggaggaatt tttaacctaa agcagcccgt gcctacgcaa actctccgcc
       61 aggatcgtct cgatcagctc ttcctggctc agcccggcca gcgccgccga gcggccgtag
      121 accttctggg accagaggtt ggcgttcaaa ttgacctcga ggaacacgac cttgccgctc
      181 tcgcggtcga agcggaactc gtagcggcca tagtcgaatg ggcggaactc ctcggcgatc
      241 cggcgggtat agtccgcgat caccggcatc cattccacgt cgtcgaactc cacgagctgg
      301 tatttctggc tccggtcgac gaggtcgcgc ttctcgtaat aggttcggag atgcgaggga
      361 tcggcctgct cgaacagcat catcggcatg atcgccggtt cgccgcggat cgtgatcacc
      421 ggcacctcga cgtcgcttcc gttgaggaag ggctcgacga tggcgtcatg gccttccgaa
      481 tggatgctgg cgatcgccgc ctcgaccccg gcccagtcgt gcgcgtcctg gaccccccag
      541 ctcgccgagg aggcgttggg cttgatcacc atccgctcgc cttccgggca gcgcgcccgc
      601 tcgaccgggg cgccgcggcg gtagatcgcc cagggcgcgg tcgggatccc gcgggcggcg
      661 accgccatct tggtcagatg cttgtcgtca gacatgcccc tcaggatcgg gctcgctccg
      721 aggaagggga tggagtgccg ggtgcacagc aggggcagca tcatctccga gttgaggaag
      781 ccgccccggt tgagcagcgg gaacacgaag tcgacctccg gcttctcgaa caaggcctca
      841 tatttgttcg ccagctcgag gttcagcccc aggctcttca atatctcccg cacctcgaca
      901 tggtagaggg cgtgattgcc gtcctcgggg tgaagcccgc cctcccacag agcgtgcttg
      961 gcgatgaaca tgatgcgcag ccgctctctg gcttcggtcg ggatggtgag cgggcggatg
     1021 tttgacacgc gggacttcgc ctttcaggga ggggcgcggc ggacatagtg ccgccgccac
     1081 tgaaggccaa gagcgcatcc ccgtgacctc ccccaccccc gcccccggcg acccggccgc
     1141 gctgatgacg accggcggcg acgcgcggat cgtgctgaag cccgaaaccg gcctcaaccg
     1201 ctaccattcg gcgcctcggc cgagcgacgt gctggcttac gcctcctcca ccgccaacga
     1261 catttcgacc cccgccttcg cccgggtcga ggctttgctc gccgggctcg ggcccgacct
     1321 ttccggccgg gcctatgccg cgcggctcga ggccctccgg gtccggatcc gcagcgccta
     1381 tgcgctcgcc ggcgaggtcg acatcgtctt cgccccgtcc ggcaccgacc tcgaatatgt
     1441 cgccttggcc tgcgccgccg gccgcggccg gaccgggatc cacaatatcc tgctcggcgc
     1501 cgacgaggtc ggaagcggct gcgtccattc cgccttcggc cgctatttcg cgcgcgagac
     1561 ggcgcttgga gtggcgacat cgcccggcga gccggttccc ggcctcggcg agacccatgt
     1621 cgagatgatc gacgtgccgg tccgcgacgg ccatggccgc gcccgtccgt cggcggaggt
     1681 cgcgggccgg atggatgtcg cgatcgccga agccgaagcc gccggccgcc acgtcctcgt
     1741 ccatgtcgtc cacggctcga agaccgggct gatcctgccc tcgctgggcg atatcgaccg
     1801 cctcgccgaa agccatggca aggccgtcac cccggtcgtc gacgcctgcc aggcgcggat
     1861 cacaagcccc gccatcgccg actatctgga gcgcggggcg atcgtcttcg tcaccggctc
     1921 caagttcatg ggcggtcccc cgttcagcgg cttcgccctc gttccccgcc gcttccgtga
     1981 agaatcggac ccgatcgccc acggcctcgc cacc
//
LOCUS       DASWNU010000643         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_24266, whole genome shotgun sequence.
ACCESSION   DASWNU010000643 DASWNU010000000
VERSION     DASWNU010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24266"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VGB57_12910"
     CDS             <1..795
                     /locus_tag="VGB57_12910"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY0132292.1"
                     /translation="PARHRAFSTPLEMMAFVARLRKLSGGKPVGFKLCLGHPWEFTGI
                     VKAMRETGTVPDFIVVDGAEGGTGASPLEFSNHVGVPLREGLLFVHNILVGAGLRDRI
                     RIGAAGKVVSAFDIASLLALGADWTNAARGFMFAIGCIQSLTCNTNTCPTGVATQDPM
                     RQRALVVGDKAERVFNFHRNTLRALSEMIAAAGLEHPSQIRPHHLVRRVSEAEVRLFS
                     QLHVFLEPGELLGGRCKRDFYGHAWALGRPDSFDLPDAQAYRGFAG"
     gene            895..1968
                     /locus_tag="VGB57_12915"
     CDS             895..1968
                     /locus_tag="VGB57_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEY0132293.1"
                     /translation="MHRLLKHWTWIAPASGWAFIALSFATGVPSLLLAAALILTVLAA
                     VHHAELIAHRVGEPFGTFLLAVAVTIIELGLIVTLMSAGGEDSATLARDTVFAAVMII
                     LNGLVGICILIGALRHREQSFQQTGVSASLATLCALTILTLVLPNFTVSEPGPVYAPS
                     QLAFVAIVSFALYITFVSVQTVRHRDYFLPPRALASDEEAHADPPSNRQTLNSSVLLV
                     ACLGAVVLLAKTLAPPIEAGVAALGAPRTVVGVIIAALVLLPESVAAVRAALANRLQT
                     SLNLGLGSALATIGLSIPAVAALSLAVGLPIALGLDAKSIVLLFLTLLVATLSLGTGR
                     TTILQGAVHLVIFGVYLFTTIVP"
BASE COUNT          269 a          780 c          579 g          347 t
ORIGIN      
        1 ccggcccggc accgcgcctt ctcgaccccg ctcgaaatga tggcgttcgt cgcccggctg
       61 aggaagcttt cgggcggaaa accggtcggc ttcaagcttt gcctcggcca tccctgggag
      121 ttcaccggaa tcgtcaaggc gatgcgcgag accgggaccg tccccgattt catcgtcgtc
      181 gacggggccg agggcggcac cggcgcctcc ccgctcgaat tctcgaacca tgtcggggtg
      241 ccgctgcgcg aggggctgct cttcgtccac aacattctcg tcggagcggg gctgagggac
      301 cggatccgga tcggagcggc cggcaaggtc gtcagcgcct tcgacatcgc cagcctgctc
      361 gccctgggcg cggactggac caacgcggcg cgcggcttca tgttcgccat cggctgcatc
      421 cagtcgctga cctgcaacac caacacctgc cccaccggag tcgcgaccca ggatccgatg
      481 cgccagcggg cgctggtcgt cggcgacaaa gcggagcggg tgttcaactt ccaccgcaac
      541 acgcttcgcg ccttgtccga gatgatcgcc gcggcggggc tcgagcatcc ctcccagatc
      601 cgcccgcacc atctggtcag gcgcgtctcg gaagccgagg tgcggctgtt ctcgcagctc
      661 cacgtcttcc tcgagccggg cgagcttctc ggcggccggt gcaagcgcga cttttacggc
      721 cacgcctggg cgctcggccg ccccgacagc ttcgacctcc ccgacgcgca ggcctatcgg
      781 ggcttcgccg gctgatacct aaatcctccc cgccctcccc ggcaaaagcc ggggaggaat
      841 ttcagcgccc gctccccggt agcccgcggc cggttcgggc gttatgcgga acgcatgcac
      901 aggctcctga agcactggac ctggatcgcc cccgcgtcgg gctgggcctt catcgccctg
      961 agcttcgcga ccggagtccc ttccctcctg ctcgcggcgg cgctgatcct caccgttctc
     1021 gccgccgtcc accatgccga gctcatcgcc catcgcgtcg gcgagccgtt cgggaccttc
     1081 ctcctggcgg tggcggtgac gatcatcgag ctcgggctga tcgtcaccct gatgtcggcc
     1141 ggcggcgagg actcggcgac cctggcccgg gacacggtct tcgccgcggt catgatcatc
     1201 ctgaacggcc tcgtcgggat ctgcatcctg atcggcgccc tccgccaccg cgagcagagc
     1261 ttccagcaga ccggggtcag cgcctcgctc gccaccctgt gcgccctcac catcctgacc
     1321 ctggttctcc cgaatttcac cgtcagcgag cccggcccgg tctacgcgcc gtcgcagctg
     1381 gccttcgtcg cgatcgtctc cttcgcgctc tacatcacct tcgtcagcgt ccagacggtc
     1441 cgccaccgcg actatttcct ccctcccagg gccctggcct cggacgagga agcccatgcc
     1501 gatccgccct cgaaccgcca aaccctgaac tcgtccgtcc ttctggtcgc ctgccttgga
     1561 gcggtcgtcc tcctcgccaa gaccctggcg ccgccgatcg aggcgggagt ggccgctctg
     1621 ggcgcaccta ggaccgtcgt cggagtgatc atcgcagctc tggtgcttct cccggaaagc
     1681 gtcgccgccg ttcgcgccgc cctcgccaac cgcctccaga ccagcctcaa cctcggcctc
     1741 ggctccgcac tcgccaccat cggcctctcc atccccgccg tcgccgcctt gtcactcgcc
     1801 gtcgggctcc ccatcgcgct cggcctcgac gccaagagca tcgtcctcct cttcctcacc
     1861 ctcctggtcg cgaccctctc cctcggcacc ggccgaacca ccatcctcca gggcgccgtc
     1921 cacctggtca tcttcggagt ctacctgttc accaccatcg tgccgtgaag gagag
//
LOCUS       DASWNU010000644         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_24279, whole genome shotgun sequence.
ACCESSION   DASWNU010000644 DASWNU010000000
VERSION     DASWNU010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24279"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VGB57_12920"
     CDS             complement(<1..981)
                     /locus_tag="VGB57_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEY0132294.1"
                     /translation="MKANVSALALAICLAACTNASADTSVDTASATPAEAPAAAAAAA
                     PTVEEARAFVARAEKELAEFSVLNSRAQWVNSTYITDDTDALAAHFGTIGTEMSVRFA
                     NEAARFANVQGLDYDTQRKLNILRSALVLPAPTTPGAAAELNTISTRLKSTYGKGRGT
                     LNGQTLSGNDLEEKMGTVRDPKQLQEMWTSWHTNVGTPMRADYTKMVGIANQGAGELG
                     FKDVGAMWRSGYDMPADEFAALTDKLWGQVKPLYDDLHCYTRTKLNQKYGAAVQPATG
                     PIRADLLGNMWAQEWGNIYDIVAPQGAGDIGYDVTDLLVAKKYDPVKIVKT"
     gene            complement(1019..1804)
                     /locus_tag="VGB57_12925"
     CDS             complement(1019..1804)
                     /locus_tag="VGB57_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0132295.1"
                     /translation="MSGSEPTTHRFTASDGTELAWHELGEGRAVLLLHGLFSSADMNW
                     IRFGHAAGIASRGLRVIMPDLRAHGESAAPHDEASYPPDVLARDALELIAHLGLGDYD
                     LGGYSLGARTSARMAIMGARPKRLVVAGMGLRGMLETGRRSAHFRKVLTGMGTHERGS
                     NEWMAEAFLKTVGGDPEALLPLLGSFVDSSEAELRTIAMPTLVLSGAEDQDNGPAEEL
                     AELLPDARFVEVPGNHMNAVTKPELGRAIADFLSLDSAPSEEQ"
BASE COUNT          293 a          601 c          705 g          346 t
ORIGIN      
        1 ggtcttcacg atcttgaccg gatcgtactt cttggcgacg agcaggtcgg tcacgtcata
       61 gccgatatcg ccggcgccct gcggcgcgac gatgtcgtag atgttgcccc attcctgcgc
      121 ccacatgttg ccgagcaggt cggcgcggat cgggccggtc gcgggctgga cggcggcgcc
      181 gtatttctgg ttgagcttgg tgcgggtgta gcagtggagg tcgtcgtaga gcggcttgac
      241 ctggccccag agcttgtcgg tcagcgccgc gaactcgtcg gccggcatgt cgtagcccga
      301 gcgccacatc gcgccgacat ccttgaagcc gagctcgccg gcaccctgat tggcgatgcc
      361 gaccatcttg gtataatcgg cgcgcatcgg agtgccgaca ttggtgtgcc agctggtcca
      421 catctcctgc agctgcttcg ggtcgcggac cgtccccatc ttctcctcga ggtcgttgcc
      481 cgagagggtc tggccgttga gcgtgccccg gcctttgccg taggtagact tcaggcgcgt
      541 ggagatggtg ttgagctcgg cggcggcgcc cggagtcgtc ggcgccggca ggacgagcgc
      601 gctgcgcaag atgttgagct tgcgctgggt gtcgtaatcg aggccctgga cgttggcgaa
      661 gcgcgcggcc tcgttagcga agcgcacgga catctcggtg ccgatcgttc cgaaatgggc
      721 ggcgagagca tcggtatcgt cggtgatgta ggtggaattc acccactggg cgcggctgtt
      781 gaggaccgag aattcggcca gctccttctc ggcccgggcg acgaaggcgc gggcctcttc
      841 cacggtcggt gcggccgcgg cggcggcggc gggcgcttcc gccggagtgg ccgaagccgt
      901 gtcgaccgag gtgtccgccg aagcattggt gcaagcggcg aggcagattg cgagggcgag
      961 agcggagaca ttggctttca tcgggtaagc atcctcgttt ctggtcttgt tggggcggct
     1021 attgctcctc ggacggggcc gaatcaagcg agaggaaatc ggcgatggcg cggccaagct
     1081 cgggcttggt caccgcgttc atatggttgc ccgggacttc gacgaagcgg gcgtcgggga
     1141 gcagctcggc gagctcctcg gcgggcccgt tgtcctggtc ctcggcgccg gacaggacga
     1201 gggtgggcat tgcaatggtt cgaagctcgg cctcgctcga atcgacgaag ctgccgagca
     1261 gcggcagaag cgcctccggg tcgccgccga cagtcttgag gaaggcttcc gccatccact
     1321 cgttcgagcc gcgttcgtga gtccccatgc cggtcaggac cttgcggaaa tgggccgagc
     1381 ggcggcccgt ctcgagcatc cctcgaaggc ccattccggc gaccaccagc cgctttggcc
     1441 tggctcccat gatcgccatc cgggccgagg tccgggcgcc gagcgaatag ccgccgaggt
     1501 cgtaatcgcc gaggccgaga tgcgcgatca gctcgagcgc gtcgcgggcg agcacgtccg
     1561 gcggatagga agcctcgtcg tgcggagccg cgctctcgcc gtgcgcgcgc aggtccggca
     1621 tgatcacccg caatccgcgc gaggcgatcc cggcggcgtg gccgaagcgg atccagttca
     1681 tgtcggcgct cgagaagagg ccgtggagga gcaggaccgc cctcccctcg cccagctcgt
     1741 gccaggcgag ctcggtgcca tcggaggctg tgaagcggtg ggtggtgggt tcggagccag
     1801 acatcattca accgtcattg cgaggagcga agcgacgaag caatccagcg ggcggagatg
     1861 cagcactgga ttgcttcgct tcgctcgcaa tgacgggctt ccgggcctaa tccggcacct
     1921 tgccggtggc ggccatccag cggtc
//
LOCUS       DASWNU010000645         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_128925, whole genome shotgun sequence.
ACCESSION   DASWNU010000645 DASWNU010000000
VERSION     DASWNU010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128925"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGB57_12930"
     CDS             complement(<1..415)
                     /locus_tag="VGB57_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132296.1"
                     /translation="MSGLVRLLAARTLSAASRLLPPHLSSWSRAMAHELMAIQDDRSA
                     LRFAAGCLRAAMSLAIAERLKAVRAILFPPSPSPRSFPIMKRILAQPRSVGLICGAVA
                     VGTGMAYMGATGAPSRYLLVNFAALVLGATAWLAVG"
     gene            complement(412..747)
                     /locus_tag="VGB57_12935"
     CDS             complement(412..747)
                     /locus_tag="VGB57_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0132297.1"
                     /translation="MTRKRALSGPARALLAALAAAGRDWSYGYELSNSTGIRSGTLYP
                     LLIRLEAQGYLEAEWQPPVAAGRPPRHAYRLTAAGQALARAEAAPDPSVAPRPSVATA
                     GRRAGEAAS"
     gene            932..2482
                     /locus_tag="VGB57_12940"
     CDS             932..2482
                     /locus_tag="VGB57_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY0132298.1"
                     /translation="MTEAEAPSPPPAIPPGFGRVALYVLLGFSAGLPFYMFSTVLALR
                     LQAHGVVLVVIGFFAWVQLLPTFKFLWAPVLDRYDVPGFARFWGKRRGWIMLSQLGIF
                     SSLVAMALTSSDSNLAVTALFAVLLAFWTTNLEIAADAWRIELAPTQDEQGPIAAANL
                     WGYRTAMVAAGSGALLVADPSGNAAPWLASLGIEAGWATAYLLIAAAAFVPLPILASM
                     RPDPGRSGGRGRALATGLAASAAILLAVLAVTAAVGWLVLGAAGGAGIDSKTNVTPYV
                     LAICMVPFLIMAAALPRIRRAPPDSAIRSSAAIGPYVDFFWRYGFGALVVMAFVSIYR
                     MGDVLALNLSKPMIKGLGYSLDQIGYADSLVALASSIVGVGLGGLIVVYGRMGIALAV
                     GAAFAAFGNFGFVWLAHQPVDVWMLYLATAADQFGNGMAGTIFVVYLSMLVNPRFPGA
                     QYAFLSGFAFLLPRLLSGAGGAIVKQLDDAGLNGFDLFFILSGVLSLAAILFLPFLAR
                     IAPRPDDR"
     gene            2479..>2768
                     /locus_tag="VGB57_12945"
     CDS             2479..>2768
                     /locus_tag="VGB57_12945"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0132299.1"
                     /translation="MIKAAFAPAATAVADGRIPGAALGIVGADGRRWVKLAGMAALVP
                     EPEPLTEAHWFDLASLTKIVATTTMILRLAEEGRLDLDRPLTDAIPDLRQYD"
BASE COUNT          389 a          999 c          903 g          477 t
ORIGIN      
        1 gcccgaccgc gagccaggct gtcgctccca gcaccagggc cgcgaaattc accaggaggt
       61 agcgcgacgg agctccggtc gcccccatgt acgccatccc cgtcccgacg gcgaccgcgc
      121 cgcaaatgag gccgaccgag cgaggttggg caagaatgcg tttcatgatc gggaagctcc
      181 ggggcgatgg tgaagggggg aagaggattg cgcggaccgc cttgagccgc tcggcgatcg
      241 ccagcgacat cgcggcgcgc aggcatccgg cggcgaaacg cagggccgag cggtcgtctt
      301 gtattgccat cagctcatgc gccatcgctc gcgaccagga cgaaagatgc ggcgggagca
      361 ggcgcgacgc ggccgacaag gtccgcgccg ccaggagacg gacgaggccg ctcacgacgc
      421 cgcctcccct gcccgtcggc ccgctgtcgc aaccgagggc cttggcgcaa cggaaggatc
      481 cggcgccgct tccgcccgcg ccagggcctg gccggcggcg gtcagccgat aggcatgacg
      541 cggcggccgg cccgccgcga ccggcggctg ccactcggcc tcgagatagc cttgagcctc
      601 gagacggatg agaagcggat agagcgtgcc tgaccggatg ccggtcgaat tcgacaattc
      661 gtagccgtag ctccagtccc ggcccgctgc ggccagcgcc gcgagcaggg ctcgggcagg
      721 tccggacaga gcgcgctttc gagtcatcca gctatatatc tacctatgta gatttatggt
      781 caagctcaat ctcgatatct caagctcctc gatctgccgc cgggcctgct catgaacttc
      841 ggcggcggga cctttcgcga ggggctgaag cgggtcgcga acaagcatcg acattgactg
      901 ttccgcgccg gcagtgaagc ttcgcgcgca gatgactgaa gccgaagcac cctccccgcc
      961 gcccgccatc ccccccggct tcgggcgggt ggcgctctac gtcctgctcg gcttctccgc
     1021 cggcctgccc ttctacatgt tctcgacggt cctggcgctg aggcttcagg cgcatggcgt
     1081 ggtgctggtc gtgatcggct tcttcgcctg ggtgcaattg ctgcccacct tcaagttcct
     1141 gtgggcgccg gtgctcgacc gctacgacgt gcccggcttc gcccgcttct ggggcaagag
     1201 gcgcggctgg atcatgctgt cgcagctcgg catcttctcc tccctggtgg cgatggctct
     1261 gacctcgtcc gactcgaatc tcgcggtcac cgccttgttc gcggttctgc tcgccttctg
     1321 gaccacaaat ctcgagatcg cggccgacgc ctggcgaatc gagctcgctc cgacccagga
     1381 cgagcagggg ccgatcgccg ccgccaattt gtggggctac cgcaccgcca tggtcgcggc
     1441 gggaagcggc gcgctcctcg tcgccgatcc gtcgggaaac gccgcaccct ggctggccag
     1501 cctcggaatc gaggcgggct gggcgaccgc ctatctgctg atcgccgccg ccgccttcgt
     1561 cccgcttccg atactcgcgt cgatgcgccc cgatccgggc cgaagcggcg gccgcggccg
     1621 ggcgctcgcg accgggctcg ccgcaagcgc cgcgatcctg ctcgccgttc tggccgtgac
     1681 cgcggcggtc ggctggctgg tcctcggagc ggccggcggc gccggcatcg acagcaagac
     1741 caacgtcacc ccttatgtgc tcgcgatctg catggtcccc ttcctgatca tggccgccgc
     1801 cctcccccgc attcgcaggg cgccccccga cagcgcgatc cggagctcgg ccgcgatcgg
     1861 cccctatgtc gacttcttct ggcgctacgg cttcggcgcc ctggtcgtga tggccttcgt
     1921 ctcgatctac cggatgggcg atgtcctcgc gctcaacctg tcgaagccga tgatcaaggg
     1981 gctcggctat tcgctcgacc agatcggcta tgccgacagc ctcgtcgctt tggcctcgag
     2041 catcgtcggg gtcgggctcg gaggcctgat cgtcgtctac ggacggatgg ggatcgccct
     2101 cgccgtcgga gccgccttcg ccgccttcgg caatttcggc ttcgtctggc tcgcccacca
     2161 gccggtcgac gtatggatgc tctacctcgc caccgcggcg gaccagttcg gcaacggaat
     2221 ggcggggacg atcttcgtcg tctacctctc gatgctggtg aacccgcgct ttccgggcgc
     2281 gcaatatgcc ttcctgtccg gcttcgcctt ccttctgccg cgcttgctgt ccggcgcggg
     2341 aggagcgatc gtcaagcagc tcgacgatgc gggactcaac ggcttcgacc tcttcttcat
     2401 cctctcgggg gtgctcagcc tggcggcgat cctgttcctt ccgttcctcg cccggatcgc
     2461 gcccaggccc gacgaccgat gatcaaggcc gccttcgctc cggccgcgac ggcggtcgcg
     2521 gacggacgaa tccccggggc ggcgctcggg atcgtcggcg ccgacggccg ccgctgggtg
     2581 aagctggccg gaatggccgc gctcgtgccc gagccggagc ctctgaccga ggcgcactgg
     2641 ttcgacctcg cctcgctcac caagatcgtc gccaccacga cgatgatcct tcgcctcgcc
     2701 gaagagggcc gcctcgacct cgaccggccg ctgaccgacg cgattcccga cctgcgccag
     2761 tacgacat
//
LOCUS       DASWNU010000646         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_206976, whole genome shotgun sequence.
ACCESSION   DASWNU010000646 DASWNU010000000
VERSION     DASWNU010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206976"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VGB57_12950"
     CDS             <1..287
                     /locus_tag="VGB57_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEY0132300.1"
                     /translation="ALVGLEPGSIVHEPRGCAQCGNSGFKGRTGVFEAVRIDDNVRRL
                     INAGGDEVEISAHAFRNSPNLAAAARQLVREGVTSVGEAVRVSRRESEDV"
     gene            280..1506
                     /gene="gspF"
                     /locus_tag="VGB57_12955"
     CDS             280..1506
                     /gene="gspF"
                     /locus_tag="VGB57_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY0132301.1"
                     /translation="MSEFAYLVLDRAGRERRGSLRDETVDESREKLSARGLFVVRMES
                     SSGEAAPPLLSRQIFARKKLNGRQLNVFTRQLSTLVMVSPLEESLRTIARQTEKDQVR
                     DVLMSVHASVLEGRRLSDAMARQPASFPPLYRGMVQAGEGSGTLPEILERLAGLLERQ
                     AQVRSKVLSALAYPAVLALVAAFVVFALMVFVVPRVVEQFEDIGQQLPFLTRAVIAVS
                     HLLAGWWWAILLGIVAIGFLANRALRDEGLRLRYDRALLRIPLIGRLLRDLHAARMAR
                     TLSTMVASRLPLLEGLKLTTETVHNRALRAATAQIAEAVRTGGSLSGALKRSGIFPPL
                     LVYLAASGEASGRLDLMLERAADYLEREFDSFTTTALALLEPAIIVLMGALVAVIVLS
                     ILLPILQLDTLAGGMG"
     gene            1503..1979
                     /gene="gspG"
                     /locus_tag="VGB57_12960"
     CDS             1503..1979
                     /gene="gspG"
                     /locus_tag="VGB57_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY0132302.1"
                     /translation="MKNFDRHPGESRDLGETRRDVLSRGPDFRRDDGERGFTLVELMV
                     VIVIIGLLATVVVINVMPATDKAAITKAKADVGVLEQGVEMYRLNKLRYPSGSEGLQA
                     VAAERYVKRLPKDPWGNAYVYAAPGRDGRQFDIYSFGADGREGGEGEDADIGNWDK"
     gene            1987..2517
                     /locus_tag="VGB57_12965"
     CDS             1987..2517
                     /locus_tag="VGB57_12965"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY0132303.1"
                     /translation="MGRAAIRTPRHPGGSRDLRTRSPGGPPRGPGFRRDDGFTLVELL
                     VVLAIIGMLSAAVIVGMPDPRGALRGEALRFAARADAARERAMMDNRPVALVLKEGGY
                     GFEWRTGGEWKPVSERPFIEQSWSEGTRAAMEGGESRIVFDSTGFAEPARLRLVRGKE
                     EAQIEVTDGGRVHVLR"
     gene            2504..2938
                     /gene="gspI"
                     /locus_tag="VGB57_12970"
     CDS             2504..2938
                     /gene="gspI"
                     /locus_tag="VGB57_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEY0132304.1"
                     /translation="MSSADDRGFTPPRRCAEHGFTPSRRSAEHGFTLVEMLVALAIFS
                     LAAMALLRLEGATVTTTALLQEQALAQTVARNLAVEALTDPEPPAFGATRGEAVNAGR
                     KWRWVRVVGRSPEPRIQQIRIEVRSDRGPEAANLTLFRRGPR"
BASE COUNT          447 a          972 c         1086 g          490 t
ORIGIN      
        1 cggcgctggt cgggctcgag cccggaagca tcgtccacga gccgcgcggc tgcgcccaat
       61 gcgggaacag cggcttcaag ggccgcacgg gcgtgttcga ggcggtccgg atcgacgaca
      121 atgtccggcg cctgatcaac gccggcggcg acgaggtcga gatctcggcc cacgccttcc
      181 gcaacagccc caatctcgca gcggcggccc ggcagctcgt tcgcgaaggc gtgaccagcg
      241 tcggcgaggc cgtgcgggtg tcgcggcgcg agagcgagga tgtctgagtt cgcctacctc
      301 gtcctcgacc gcgccgggcg cgagcggcgc gggtcgcttc gcgacgagac ggtggacgaa
      361 tcgcgggaga agctgagcgc ccgcggattg ttcgtggtca ggatggagag ctcgagcggc
      421 gaggccgcgc cgcccttgct ctcccgccag atcttcgccc gcaagaagct gaacggccgc
      481 cagctcaacg tcttcacccg gcaattgtcc accttggtga tggtgagccc gctcgaagag
      541 tcgcttcgaa ccatcgcccg ccagaccgag aaggaccaag tccgcgacgt cctgatgtcg
      601 gtccacgcct cggtcctcga agggcggcgg ctttccgacg cgatggcgcg ccagccggcc
      661 agctttcctc ccctctatcg cggaatggtc caggcgggcg aggggtcggg tactctgccc
      721 gagatactgg agcggctcgc cgggctgctc gagcggcagg cgcaggtgcg tagcaaggtt
      781 ctgagcgctc tggcttatcc cgccgtgctc gcgctcgtcg ccgccttcgt cgtgttcgcg
      841 ctgatggtgt tcgtcgtgcc ccgagtggtc gagcagttcg aggatatcgg ccagcagctg
      901 cctttcctca cccgggcggt gatcgccgtc tcgcacctcc tcgcgggatg gtggtgggcg
      961 atattgctgg gcatcgtggc gatcggcttc ctcgccaatc gggcccttcg cgacgagggc
     1021 ctgcgcctgc gctacgaccg ggcgctgctt cggatcccgc tgatcggccg gcttcttcgc
     1081 gatcttcacg ccgcccggat ggcgcgcacc ctctcgacca tggtggccag ccggctgccc
     1141 ttgctcgagg ggctgaagct gaccaccgag acggtgcaca accgcgccct gcgcgcggcg
     1201 acggcgcaga tcgcggaagc ggttcgcacc ggcggcagcc tgtcgggggc actcaagcgc
     1261 tcgggaatct tcccgccctt gctcgtctat ctcgccgcaa gcggggaggc gagcggacgg
     1321 ctcgacctga tgctggagcg cgccgccgat tatctcgagc gcgagttcga cagcttcacg
     1381 accaccgcgc ttgccttgct cgagcctgcg atcatcgttc tcatgggcgc tctggtcgcc
     1441 gtgatcgtgc tttcgattct gctgccgatc ctgcagctcg acacacttgc cggaggaatg
     1501 ggatgaagaa tttcgatcgt catcccggcg aaagccggga cctcggggag acccgccgag
     1561 acgtcttgtc ccggggtccc gactttcgcc gggatgacgg ggaacggggc ttcaccctcg
     1621 tcgaattgat ggtggtcatc gtcatcatcg gactgctcgc caccgtcgtc gtcatcaacg
     1681 tcatgccggc gaccgacaag gcggcgatca ccaaggccaa ggccgacgtc ggggtcctcg
     1741 agcagggcgt cgagatgtat cgcctcaaca agctgcgcta tccgagcggg tcggaaggcc
     1801 tgcaggcggt cgcggcggaa cgctatgtga agcgcctgcc caaggatccg tggggcaatg
     1861 cctatgtcta tgccgcgccg ggccgcgacg ggcgccagtt cgacatctac agcttcgggg
     1921 cggacggccg cgaagggggc gaaggcgagg atgcggacat cggcaactgg gacaagtgag
     1981 gcttgaatgg ggcgggccgc gatcaggact ccccgtcatc ccggcggaag ccgggatctc
     2041 aggacgagaa gtcccggcgg gcctccccga ggtcccggct tccgccggga tgacggtttc
     2101 accctcgtcg agctgctcgt cgtcctggcg atcatcggca tgctctcggc ggcggtgatc
     2161 gtcggcatgc cggacccgcg gggggcgctc aggggggagg cgcttcgctt cgccgcccgc
     2221 gccgacgcgg ctcgggagcg ggcgatgatg gacaatcgtc cggtggcgct ggtgcttaag
     2281 gagggcggct acggcttcga atggcgcacc ggcggcgagt ggaagccggt gagcgagcgg
     2341 ccgttcatcg agcagagctg gagcgagggc acccgcgccg cgatggaagg gggggagtcg
     2401 cggatcgtgt tcgattcgac cggcttcgcc gagccggccc gactcaggct ggtgcggggc
     2461 aaggaagagg cccagatcga ggtgactgac ggagggaggg tccatgtcct ccgctgacga
     2521 cagaggcttt acgcctccga ggcgttgcgc cgaacacggc ttcacgcctt cgaggcgttc
     2581 cgccgaacac ggcttcacgc tggtcgagat gctggtggcg ctggcgatct tcagcctggc
     2641 ggcgatggct ttgctccggc tcgagggggc gacggtgacg accaccgctt tgctccagga
     2701 gcaggcgctg gctcagaccg tggctcgcaa cctggcggtc gaggcgctta ccgatccgga
     2761 gccgccggcg ttcggcgcca cgaggggcga ggcggtcaat gcggggcgca aatggcgctg
     2821 ggtccgggtt gtcggccgtt cgcccgagcc ccgcatccag cagatccgga tcgaggtgcg
     2881 gagcgaccgc gggccggaag cggcgaacct cactctgttc cgaagggggc cgcggtgaag
     2941 ggtcgcaact ttgcctcccc gcgcgaagcg gggggagggg gaccatgcgt agcat
//
LOCUS       DASWNU010000647         5997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_414061, whole genome shotgun sequence.
ACCESSION   DASWNU010000647 DASWNU010000000
VERSION     DASWNU010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414061"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..784)
                     /locus_tag="VGB57_12975"
     CDS             complement(74..784)
                     /locus_tag="VGB57_12975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0132305.1"
                     /translation="MSLIHTVAETGSTNDDLASLARQGAPEGVWLRAGRQSSGRGRQG
                     REWHSPPGNLYASTLVRLRPGDPPAPTLALVAAVALHEVACAFAVVGVTIEIKWPNDL
                     LVAGAKLSGILLERIDDAIVLGFGVNLADHPDEIARPAINMGMLGGAPDPARFLDALA
                     ASFARWVGRWRDEGLEPVRLRWLAAAHPVGTALSTRTAGGARIEGLFDGLDESGALRL
                     RLPDGTAQVIHAGDVFLV"
     gene            complement(804..1394)
                     /locus_tag="VGB57_12980"
     CDS             complement(804..1394)
                     /locus_tag="VGB57_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0132306.1"
                     /translation="MGERRAYIIEDEKLLRDLVRQSLAGCCTRIEEYGSAESFLAGHE
                     DRPVGCIIVDINLPGINGLDLLEVIAKGRAAYPVIVVSGNGNVPHAMRAGRLGVVDFI
                     EKPFRVDQLLDAVEKGFALLESQPMSRISALATLTRREQEVLVAFADGAPNKIVAHDL
                     GLSVRTVELHRANLMKKLGVRSLSQALLIAKDGGYV"
     gene            complement(1589..3025)
                     /gene="nuoN"
                     /locus_tag="VGB57_12985"
     CDS             complement(1589..3025)
                     /gene="nuoN"
                     /locus_tag="VGB57_12985"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY0132307.1"
                     /translation="MTASLLLTLPEIVLTLAALALMMAAAWMGDKSARLLTWLGIAAL
                     AGAAFAIPGISGRGGTAFDGLYIGDSFAAFAKVLIYIAAAASLVAASGWFSRDRDYRA
                     EYPCLILLSSVGMGMMVSAGDLLTLYVGLELQSLSAYVLASFQRRDNRSAEAGLKYFV
                     LGALASGILLYGVSLLYGFTGSTLFGEINAALGRDGVSTGELFGIVFVLAGIAFKVSA
                     VPFHMWTPDVYEGAPTPVTAFFASAPKVAAMALLTRVAVEAMGPATFEWRQIVIFMAL
                     ASTILGGVAAIGQPNIKRLLAYSSINNVGFALIGLAAATRYGVASVLFYMAVYTVMTL
                     GTFLVVLRMRDSDGRPVESIASLSGLSTTRPWLAAGMAIFMFSLAGVPPLFGFWPKFL
                     VFDAAVRADLTWLAAVGIVTSVISAFYYLKIVKTMYFDDPAPAYAETKDVPQAVLIVL
                     AALFVSPLGYIAIPFIGRMADNAASALF"
     gene            complement(3025..4659)
                     /locus_tag="VGB57_12990"
     CDS             complement(3025..4659)
                     /locus_tag="VGB57_12990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0132308.1"
                     /translation="MIQATLEFGFPILSEMLLIPLAAAIVCMCVSANSARWVALIATF
                     VELALGILLWTQYNLAADQWQFQELYTFSKGEPLFGRFFWALGVDGIAIMLIMLTVFL
                     MPICILASWKSIERRVPEYMAAFMLVEAMTLGVFMAQDIFLFYIFFEAGLIPMFLIIG
                     IWGGAQRIYASYKFFLYTLLGSVLMLVAMLWMVNYAGTTAIPKLMVTNFPPEAQTWLW
                     LAFFASFAVKMPMWPVHTWLPDAHVQAPTAGSVILAGVLLKLGGYGFLRFSLPMFPEA
                     SAQLVWLIFALSLIAIVYTSLVALVQRDMKKLIAYSSVAHMAFVTIGLFAFNRQGIEG
                     GIIVMLSHGLVSAALFLCVGVVYDRLHTREIDRYGGLSNNMPAFAVLFLLFTMAAVGL
                     PGTSGFVGEFLALVGAYEASSLVAAVATTGIILGAAYMLWLYWRVAYGTSRNADAAAM
                     PDLDKRELASLGAIAAVVLWMGVYPEPFMSTIRRDVGFLLARIERANPPGDAQPTNKG
                     APLPKAHSPEPHAPVPAPVPEGPAPEGHSHAPGEEH"
     gene            complement(4656..>5997)
                     /locus_tag="VGB57_12995"
     CDS             complement(4656..>5997)
                     /locus_tag="VGB57_12995"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0132309.1"
                     /translation="PDAMEGPTPVSALIHAATMVTAGVFMVCRLSPMFETSQTALTLV
                     TVIGAATAIFAATVGTVQNDIKRVIAYSTCSQLGYMFFAAGVGAYGAAMFHLFTHAFF
                     KALLFLGAGSVIHAMHHEQDMRFYGALRKHIPLTFWAMTIGTLAITGVGIIQVAGWSG
                     FGFAGFYSKDAIIESAFASGTGVGGFAFVIGITAALLTSFYSWRLVFLTFFGKARWAA
                     SEHIQHAVHDHGHHGDDPAAEDSGHDTRAHEASAREVPAGTGGYHPHESPWTMLVPLG
                     VLSLGAVFAGMLFHGPFIEAEEGAHFWGASIAFDTHLAHAIHEVPLWVKLAPGTVMIV
                     GFLIALQAYIRRTDIPARFTGQWGILYNLFYNKWYFDEIYNVVFIRPSLWLGRLFWKR
                     GDEKTIDRFGPGGAATAVRVGNVLTARLQSGYLYTYALVMLLGLAAAATWAMAR"
BASE COUNT         1142 a         1878 c         2135 g          842 t
ORIGIN      
        1 gttccccggc gaaggccggg gcccagccgc gcggcccggc aaaccgggtc ccgggcctcc
       61 gccggggaac ttattacacc aggaacacgt cgccggcatg gatgacctgc gccgtcccgt
      121 ccgggaggcg gaggcgcagt gccccgctct cgtcgagccc gtcgaacagg ccttcgatcc
      181 gagcgccgcc ggccgtgcgg gtggagaggg cggttccgac cggatgggcc gcggcgagcc
      241 agcgcaggcg gacgggctcg aggccctcgt cgcgccagcg gccgacccag cgggcgaagc
      301 ttgccgccag ggcgtcgagg aaccgggccg gatcgggggc gccgcccagc atacccatgt
      361 tgatggcggg acgagcgatc tcgtcgggat ggtcggcgag attgactccg aagccgagga
      421 cgatggcatc gtcgatccgc tccaggagga tgccggacag cttggcgccg gcgaccagca
      481 gatcgttggg ccatttgatc tcgatcgtca ctcccaccac ggcgaaggcg caggccacct
      541 cgtgcagcgc cacggccgcg accagagcca gggtcggtgc cggcgggtcg ccgggacgaa
      601 gccggacgag cgtgctcgca tagagattgc ccggaggcga atgccattcc cgcccctgcc
      661 gcccgcgccc gctcgactgg cgcccggcgc gcagccagac cccctcgggc gcgccttggc
      721 gggcgagtga ggcgaggtcg tcattggtgg agccggtctc ggcgacggta tggatgaggc
      781 tcatcggcgg ctacggcttc ggctcagaca tagccgccgt ccttggcgat gaggagggcc
      841 tgggaaaggc tccgcacgcc cagcttcttc atcagattgg cgcgatgaag ctcgaccgtc
      901 cggacgctga gcccgagatc gtgggcgacg atcttgttcg gagccccgtc cgcgaaggcg
      961 acgagcacct cctgttcccg ccgagtcagg gtggcgaggg ccgagatccg cgacatgggc
     1021 tggctctcga gcaaggcgaa gcccttctcg acggcatcga gcagctggtc gacccggaac
     1081 ggcttctcga tgaagtccac gactccgagc cggcccgccc gcatcgcatg cggcacgttg
     1141 ccgttgcccg agaccacgat gaccggatag gccgcccgcc ccttcgcgat cacttcgagc
     1201 agatcgaggc cgttgatccc gggcaggttg atgtcgacga tgatgcagcc caccggtcgg
     1261 tcctcatggc cggccaggaa ggattcggcg gagccatatt cctcgatccg ggtgcagcaa
     1321 ccggccagcg actgccgcac gagatcgcgc agcagctttt cgtcctcgat gatataggct
     1381 cgccgctcac ccatttgcat tccagactag ccgccgcttc gccccgcggc cacagccgac
     1441 ataggtattt ttccgaagta ggacagagcc ggcgatggcg agcacgatgc acaggccgcc
     1501 ggggggaggc atcccgtccc ttcgcgcggc ttgtagctgc atggattggg ccggcggccg
     1561 gcgtgaccgc cggtccagtc tagcaaggtc agaacagagc gctcgcggca ttgtcggcca
     1621 ttcggccgat aaaggggatg gcgatatagc cgagcggtga gacgaacagg gccgccagca
     1681 cgatcaggac cgcctgcgga acgtccttcg tctcggcata ggccggcgcc ggatcgtcga
     1741 aatacatcgt cttgacgatc ttgagataat agaaggcgct gatcaccgag gtgacgatgc
     1801 cgaccgcggc gagccaggtg aggtcggccc tgaccgcggc atcgaagacc aggaacttgg
     1861 gccagaatcc gaacagaggc ggcactccgg cgaggctgaa catgaagatc gccattccgg
     1921 ccgcaagcca gggccgggtc gtggacaggc ccgagaggct ggcgatgctc tccaccggcc
     1981 ggccgtccga gtcgcgcatc ctcagcacca ccaggaaggt gcccagggtc atcaccgtgt
     2041 agacggccat gtagaagagc accgaagcga cgccgtagcg ggtggcggcg gcgaggccga
     2101 tcagcgcgaa gccgacattg ttgatcgagg aataagcgag cagccgcttg atgttcggct
     2161 ggccgatcgc ggccaccccg ccgaggatgg tcgaggcgag cgccatgaag atgacgatct
     2221 ggcgccattc gaaggtggcg gggcccatcg cctccaccgc gacccgggtc agcagggcca
     2281 tggccgcgac cttgggagcc gaggcgaaga aggcggtcac cggggttgga gcgccttcgt
     2341 aaacgtcggg ggtccacatg tgaaacggca ccgccgagac cttgaaggcg atcccggcga
     2401 ggacgaagac gattccgaac agctcgccgg tcgagactcc gtcccggccc agggcggcat
     2461 tgatctcgcc gaacagggtc gagccggtga agccgtagag gagcgacact ccgtagagca
     2521 ggattccgct ggcaagggcc ccgaggacga aatatttgag cccggcctcc gcagagcggt
     2581 tgtcgcgcct ctggaagctg gcgaggacat aggcggagag gctctgaagc tcgagcccga
     2641 catagagggt gaggaggtcg cccgccgaga ccatcatgcc cattcccacc gaggagagca
     2701 ggatcaggca gggatattcg gcccgatagt cgcggtcgcg gctgaaccag cccgaggccg
     2761 cgaccagaga ggcggcggcg gcgatgtaga tcaggacctt ggcgaaggcg gcgaagctgt
     2821 cgccgatata gaggccgtcg aaagccgtgc cgccgcgacc cgaaatcccg ggaatggcga
     2881 aggccgctcc ggcgagcgcc gcgattccga gccaggtgag caggcgggcg ctcttgtcgc
     2941 ccatccaggc ggccgccatc atcaaagcca gcgccgccag ggtgaggacg atctcgggca
     3001 gggtgaggag aagcgaggcg gtcatcaatg ctcctccccg ggcgcgtggc tgtgaccctc
     3061 gggcgcgggc ccctccggca ccggcgccgg gaccggcgcg tggggttcgg gcgaatgcgc
     3121 cttcggcagc ggcgcgccct tgttggtggg ctgggcgtcc ccgggcgggt tcgcccgttc
     3181 gatgcgcgcc agcaggaagc cgacgtcgcg gcggatcgtg gacatgaagg gctcgggata
     3241 gacgcccatc caaagcacca ccgcggcgat cgctccgagg gaggcgagct cgcgcttgtc
     3301 gagatcgggc atcgccgccg cgtcggcgtt gcggctcgtc ccgtaggcga cgcgccaata
     3361 aagccagagc atgtaggcgg cgcccaggat gatcccggtc gtggcgacgg cggcgacgag
     3421 gctgctcgcc tcataggcgc cgaccagggc caggaactcg ccgacgaagc cgctcgtccc
     3481 cggcagcccg accgcggcca tggtgaacag aaggaacagc accgcgaacg ccggcatgtt
     3541 gttggagagg ccgccatagc ggtcgatctc acgggtatgc agccggtcgt agacgactcc
     3601 gacgcacagg aagagcgcgg ccgagaccag gccgtggctg agcatgacga tgatcccgcc
     3661 ctcgatcccc tgccggttga aggcgaagag gccgatggtg acgaaggcca tgtgggcgac
     3721 cgacgaataa gcgatgagct tcttcatgtc ccgctgcacc agggcgacca gcgaggtgta
     3781 gacgatggcg atcaggctca gcgcgaagat cagccagacg agctgcgccg aggcttccgg
     3841 gaacatgggc agcgagaagc ggaggaagcc gtagccgccc agcttcagaa gcactccggc
     3901 gaggatcacc gagcccgccg tcggcgcctg cacgtgggcg tcggggagcc aggtgtggac
     3961 cggccacatc ggcatcttga ccgcgaagga ggcgaagaag gccagccaca gccaggtctg
     4021 agcctcgggc gggaagttgg tcaccatcag cttggggatg gcggtggtgc cggcgtaatt
     4081 caccatccac agcatggcga ccagcatcag caccgagccg agcagcgtat agaggaagaa
     4141 tttgtagctt gcgtagatgc gctgggcgcc tccccagatt ccgatgatca ggaacatcgg
     4201 aatgaggccg gcctcgaaga agatgtagaa gaggaagatg tcctgggcca tgaacacgcc
     4261 cagggtcatc gcctccacga gcatgaaggc cgccatatat tcgggaaccc gccgctcgat
     4321 cgatttccag ctggcgagga tgcagatcgg catcaggaag acggtgagca tgatcagcat
     4381 gatggcgatg ccgtcgacgc cgagcgccca gaagaagcgg ccgaacagcg gctcgccctt
     4441 ggagaaggta tagagctcct ggaactgcca ctgatcggcg gcgagattat attgcgtcca
     4501 gagcaggatc ccgagcgcca gctcgacgaa ggtggcgatc agcgccaccc agcgcgcgct
     4561 attggccgag acgcacatgc agacgatcgc cgccgccagc gggatcagga gcatctccga
     4621 caggatcgga aagccgaatt ccagcgtggc ctggatcatc tcgccattgc ccaggtcgcg
     4681 gcggcggcga ggccgaggag catcaccagc gcataagtgt agagatagcc ggactggagc
     4741 cgggcggtga ggacgttgcc gacccggacc gccgtcgcgg cgccgccggg gccgaagcgg
     4801 tcgatcgtct tctcgtcgcc gcgcttccag aacaggcgcc cgagccacag cgagggacgg
     4861 atgaagacga cgttatagat ctcgtcgaaa taccatttgt tgtagaagag attgtagaga
     4921 atcccccact ggccggtgaa gcgcgcgggg atgtccgtgc gccggatata ggcctgcagc
     4981 gcgatcagga agccgacgat catcacggtg ccgggggcga gcttcaccca caaaggcact
     5041 tcgtggatgg cgtgggcgag atgggtgtcg aaggcgatgc tggcgcccca gaaatgcgcc
     5101 ccttcctcgg cctcgatgaa cgggccgtgg aagagcattc cggcgaagac agcgccaagc
     5161 gacagcacgc ccagcggcac gagcatggtc caggggcttt cgtgcggatg atagccgccg
     5221 gtgccggcgg gcacttcgcg cgccgaagct tcgtgcgcac gggtgtcgtg gccgctatcc
     5281 tcggccgccg gatcgtcgcc atgatggccg tggtcgtgaa ccgcgtgctg gatatgctcc
     5341 gaagcggccc agcgcgcctt gccgaagaag gtcaggaaga cgagccgcca gctgtagaag
     5401 ctggtcagaa gcgccgccgt gatcccgatg acgaaggcga agccgccgac tccggtgccg
     5461 ctggcgaagg cgctctcgat gatcgcgtcc ttcgaataga agccggcgaa gccgaagccg
     5521 gaccagcccg ccacctggat gatcccgacc ccggtgatgg cgagcgtacc gatggtcatc
     5581 gcccagaagg tgagcgggat gtgcttccga agggcgccgt agaagcgcat gtcctgctcg
     5641 tgatgcatgg cgtggatgac cgagccggcg cccaggaaga gcaaagcctt gaagaaggcg
     5701 tgggtgaaga ggtggaacat cgccgcgcca taggcgccca ctcccgcggc gaagaacatg
     5761 tagcccagct gcgagcaggt cgagtaagcg atgacgcgct tgatgtcgtt ctggaccgtc
     5821 ccgacggtgg cggcgaagat cgcggtcgcg gccccgatca cggtgacgag ggtgagcgcg
     5881 gtctggctgg tctcgaacat cggggagagc cggcagacca tgaacacgcc cgcggtcacc
     5941 atggtggcgg catggatgag ggcggagacg ggcgtcgggc cctccatcgc gtccggg
//
LOCUS       DASWNU010000648         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_76651, whole genome shotgun sequence.
ACCESSION   DASWNU010000648 DASWNU010000000
VERSION     DASWNU010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76651"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..865)
                     /locus_tag="VGB57_13000"
     CDS             complement(329..865)
                     /locus_tag="VGB57_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132310.1"
                     /translation="MSAELASRRAAAAGTRFTVVPRHPPRHAHGGSRGPVAAQRLVEL
                     TEELGRLAGELAEGDGPTAAPALDSEFVRGLIRQRQRRSAAFGADLFADPVWDMMLDL
                     LAARFEGRAVRTSSLCIAAGVPSTTALRWIRELTERGLFVRGADPLDGRGVLIELSGD
                     AAQRLLDHLSAGLESASL"
     gene            complement(855..2303)
                     /locus_tag="VGB57_13005"
     CDS             complement(855..2303)
                     /locus_tag="VGB57_13005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY0132311.1"
                     /translation="MTAIAERFPTVSRHLAVLREAWRRQNETERTKKPIVEHEFLPAA
                     LEIMEKPPSPGLRLLMLSLCTLFAIALAWSFLGKVDVVAVASGKTLPAANVKLIQPIE
                     IGAVRAIHVRNGQRVRKGQLLIELDPTVAGAEEAQASRGLLTARIAEARNDAFLAHLA
                     GRPASFRTPAGTSPEVVQTQNQLIRSAVAEYEAQRASLGETRAERSAELAATSAEIAK
                     LRQTLPLVDQQLEARRDLTARGHFSKLKLLEYEQMRVEHMRNIDVQESHAGKSRAAIA
                     NIDAQLRQLREEFSKRAATDLSAAEDESGMRSEEVRKSERRRLYQQISSPVDGIVQQL
                     AVHTIGGIVQPAQPLMIIVPDDGSQVEVEAQIINRDIGFVREGQKVRVKLEAYPFTDY
                     GLVEGVVETISRDAVQQEAQPAGEGKAQAGGLMYTARIKLSRSWIQVGGRRQPIGPGL
                     AVQAEIKTGERRIIQYLVSPISQAMDEAGRER"
     gene            complement(2300..>3857)
                     /locus_tag="VGB57_13010"
     CDS             complement(2300..>3857)
                     /locus_tag="VGB57_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEY0132312.1"
                     /translation="LAFGLAVVLIWETALSGLRNWLFAHSTNRVDAELSARMFRHLLN
                     LPLSYFEARRVGDSIARVRELESIRDFLTSNAVTVVIDLFFTVVFFAVMYMYSPMLTL
                     VVLLSIPLYVAVSLFITPPLRDRLDEKFRRGAENQSFLVETVTGIGTLKAMAVEPQMR
                     DRWEKQFAGYTQTGFQVTRLANWGNHLIQGISKLTTVAILYFGAKAVIAGDLSVGALV
                     AFNMLSGRVSQPILRLSQLWQDFQQVRISVDRLGDVLNAPAEPEHNPNRASLPAIRGE
                     IGFDKVRFRYRPDAPEALRGVSLTIAAGEMLGIVGPSGSGKSTLTKLVQRLYVPEQGR
                     VMVDGVDLSLVDPAWLRRQVGVVLQENILFNRSVRENISLVDPTRPMEAVMAAAELAG
                     AHEFIVGLSHGYDTIIDERGANLSGGQRQRIAIARALINDPRILILDEATSALDAESE
                     EIIQRNLRMISHGRTVIIIAHRLSAVRQCHRIITVEAGEVTEEGDHSTLLRKGGRYST
                     LYSKQMGVAA"
BASE COUNT          605 a         1264 c         1366 g          622 t
ORIGIN      
        1 cgcccgcacg ccaacgcggc ttttgctgga cccccctcga agcactgtcg gcgcttccct
       61 tccccaaaaa atatcgcagg agccgcccgc cctcacgcgc ggcgaagacg atgacccatc
      121 cccaaagcct cgctgcctgc ctcccggccg ccgggcaagc tccaaacgca gatagttcca
      181 ctgtggaatc aatagtttgc cgagacgcgg aaaaggccga tatatacgca tccgtaacgt
      241 cccccgtttc gcgccaaatc ggcggctatc gctccattcc agacgccgcg tggcggcaga
      301 gaatcaccgg aagtccggag ccggaggatc agagcgacgc gctctcaagc ccggcggaca
      361 gatggtcgag cagccgctgc gccgcgtcgc cggacagttc gatcagaacg ccgcggccgt
      421 cgagcgggtc ggcgccccgg acgaagaggc ctcgctcggt gagctcgcgg atccagcgca
      481 gggcggtggt gcttgggact ccggccgcga tgcacaggct cgaggtgcga acggcccggc
      541 cctcgaagcg ggcggcgagc aggtcgagca tcatgtccca gaccggatcg gcgaacaggt
      601 cggcgccgaa cgccgccgag cgccgctgcc tctgccggat gagtccgcgc acgaactccg
      661 agtcgagcgc gggagcggcc gtcgggccat ccccttccgc gagctcgccg gccagccggc
      721 cgagctcctc ggtcagctcg acgagcctct gggccgcgac cgggccgcgc gagcctccat
      781 gtgcatgacg cggaggatgg cgcggaacga ccgtgaagcg ggtccccgcc gccgccgctc
      841 gccggctcgc caactcagcg ctcacggccg gcctcgtcca tcgcctggga aatgggggat
      901 acgagatact ggatgatccg gcgctccccg gtcttgatct cggcctggac ggcgagtccg
      961 gggccgatcg gctggcggcg gccgccgacc tggatccagc ttcgggacaa tttgatccgg
     1021 gccgtataca tgaggccgcc tgcctgcgcc ttgccctcgc cggccggctg cgcctcctgc
     1081 tggacggcat cgcggctgat cgtctcgaca acgccctcga cgaggccgta gtcggtgaac
     1141 ggataggctt cgagcttgac ccggaccttc tggccttcgc ggacgaagcc gatgtcgcgg
     1201 ttgatgatct gcgcttccac ctcgacctgg ctgccgtcgt ccggaacgat gatcatcagc
     1261 ggctgggcgg gctggacgat tccgccgatc gtgtggacgg cgagctgctg gacgatgccg
     1321 tcgaccggcg agcttatctg ctggtagagg cggcgccgct cggacttcct gacctcctcg
     1381 cttcgcatcc cggattcgtc ctcggccgcc gaaaggtcgg tcgccgccct cttcgagaat
     1441 tcctcgcgga gctggcgaag ctgggcgtcg atgttggcga tcgcggcgcg cgacttgccg
     1501 gcatggcttt cctggacgtc gatgttgcgc atgtgctcga cccgcatctg ctcatattcg
     1561 agcagcttga gcttcgagaa atggccccgg gcggtgaggt cgcggcgggc ctcgagctgc
     1621 tggtcgacga gcggcagggt ctggcgcagc ttggcgatct cggccgaggt cgcggcaagc
     1681 tcggccgacc gctcggctcg ggtctcgccg agcgacgctc gctgcgcctc atattcggcg
     1741 acggcgctcc ggatcagctg gttctgggtc tggacgacct cgggcgaagt gccggcggga
     1801 gtccggaacg aagcggggcg gccggcgagg tgggcgagga aagcgtcgtt gcgcgcctcg
     1861 gcgatccggg cggtgaggag gccccggctg gcctgggctt cctcggcccc ggcgacggtc
     1921 gggtcgagct cgatgaggag ctgccccttg cggaccctct ggccgttgcg gacgtggatg
     1981 gcgcgcaccg cgccgatctc gatcggctgg atcagcttga cgttggcggc cggaagggtc
     2041 ttgccgctgg ccaccgcgac gacgtcgacc ttgcccagga acgaccaggc gagggcgatc
     2101 gcgaagagcg tgcacaggct gagcatcagc aggcgaaggc ccgggctcgg cggcttctcc
     2161 atgatctcga gcgccgcggg gaggaattcg tgctcgacga tcggcttctt cgtccgctcg
     2221 gtctcgttct ggcggcgcca ggcctcgcga agcacggcca ggtggcggga cacggtcggg
     2281 aaacgttcgg cgatcgcggt catgcggcca ctcccatctg cttgctgtac agggtgctgt
     2341 agcggccgcc tttccggagc agcgtcgagt gatcgccttc ctcggtgacc tcgccggcct
     2401 cgacggtgat gatccggtgg cactggcgga cggcggaaag gcggtgggcg atgatgatca
     2461 ccgtccggcc gtggctgatc atcctcaagt tgcgctggat gatctcctcg ctctcggcgt
     2521 cgagggcgga ggtcgcctcg tccaggatga ggatgcgcgg gtcgttgatc agcgcccggg
     2581 cgatggcgat gcgctggcgc tggccgccgg aaaggttggc gccgcgctcg tcgatgatcg
     2641 tgtcgtagcc gtgggagagg ccgacgatga actcatgcgc tccggcgagc tcggcggccg
     2701 ccatcaccgc ctccatcgga cgggtcggat cgaccagcga gatgttctcg cggacgctcc
     2761 ggttgaacag gatgttctcc tgaagcacca ctccgacctg ccggcgaagc caggcgggat
     2821 cgaccagcga gaggtcgact ccgtcgacca tcacccggcc ctgctcgggc acgtagaggc
     2881 gctggacgag cttggtcagg gtcgacttgc cggagccgga cgggccgacg atgccgagca
     2941 tctcgcctgc cgcgatggtg aggctgactc cgcgcagagc ctccggagcg tcgggacgat
     3001 agcggaagcg gaccttgtcg aacccgatct cgccgcggat cgcgggcagg ctggcgcgat
     3061 tcggattatg ctcgggctcg gcgggggcgt tcaggacgtc gccgagccgg tcgaccgaga
     3121 tccggacctg ctggaagtcc tgccagagct gggagaggcg caggatcggc tgcgataccc
     3181 ggccggacag catgttgaag gcgacgagcg ctccgacgct gagatcgccg gcgatcaccg
     3241 ccttcgcccc gaaatagagg attgcgaccg tggtgagctt ggagatgccc tggatgaggt
     3301 ggttgcccca attggcgagc ctggtcacct ggaagccggt ctgggtatag ccggcgaact
     3361 gcttctccca gcggtcgcgc atctgcggct cgaccgccat cgccttcagt gtgccgatgc
     3421 ccgtcaccgt ctcgaccagg aaggactggt tctcggcgcc tcggcggaac ttctcgtcga
     3481 gccggtcgcg cagcggcggg gtgatgaaca gtgaaacggc gacgtagagg ggaatcgaca
     3541 gcaggacgac caacgtcagc atcgggctgt acatgtacat cacggcgaag aagacgacgg
     3601 tgaagaagag gtcgatgacc acggtgaccg cgttcgacgt caggaagtcg cggatgctct
     3661 cgagctcgcg cacccgggcg atgctgtccc cgacccgcct cgcctcgaaa taggagagcg
     3721 gcaggttgag caggtgccgg aacattcggg cgctgagctc cgcgtccacc cggttcgtgc
     3781 tgtgcgcgaa cagccagttg cggaggccgg aaagggccgt ttcccagatc agcaccaccg
     3841 ccaggccgaa cgccagc
//
LOCUS       DASWNU010000649         5521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_51069, whole genome shotgun sequence.
ACCESSION   DASWNU010000649 DASWNU010000000
VERSION     DASWNU010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51069"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VGB57_13015"
     CDS             <1..919
                     /locus_tag="VGB57_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY0132313.1"
                     /translation="EAASASELRENLVAESGFYVPAIDWTRTSRRVLTLEWIDGIKLT
                     DRDALVAAGHDLKSLAAILVRAFLRQSVIDGFFHADLHQGNLFALPDGRLAAIDFGIM
                     GRIDRQARMWLAEILYGLLTGNYRRVAEIHFEAQYVPGHHDVDEFATALRAVGEPIRG
                     LPVKDISIGQMLDGLFTITRDFEMPTQPHLLLLQKTMVMEEGVAHALDPDINMWETAE
                     PFLREWVRGELGPEAALADRIVRDFRTFAKIPELIRRIDHYYPPPGAAPPDPPLTDVI
                     VVKPRRYWRYALTAIGSAAAGAAAVWLLL"
     gene            924..1217
                     /locus_tag="VGB57_13020"
     CDS             924..1217
                     /locus_tag="VGB57_13020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0132314.1"
                     /translation="MTRGDVLDALRPLEPELKRLGFAKLYLFGSAARSEPNARDVDLL
                     YEAEGALKPGFNELADAWDRLESVLGKPVDLVDIELLHQRIRPRVENEMVEVY"
     gene            1217..1570
                     /locus_tag="VGB57_13025"
     CDS             1217..1570
                     /locus_tag="VGB57_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEY0132315.1"
                     /translation="MNAGPEADALYLDQIVELIQLIRESVVGTDEAEFLLDRTKGDAT
                     ALRLGAIGEASRKLSEELQERHPEIEWRRMYGLRNIVAHHYRKLNYRILWQIATSTLD
                     PLENACRDELSRFDG"
     gene            1567..2796
                     /gene="coaBC"
                     /locus_tag="VGB57_13030"
     CDS             1567..2796
                     /gene="coaBC"
                     /locus_tag="VGB57_13030"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY0132316.1"
                     /translation="MNGNRILLIVGGGIAAYKACELIRLIRKASGSVRCVLTAGGAQF
                     VTPTTLAALSEQPVHTSLWDLKDEVEMGHIQLSREADLVVVCPATADLMAKMAAGIAD
                     DLATTLLLATDKPVLAAPAMNVRMWRHEATQRNVARLRSDGVTILEPDEGEMACGEYG
                     PGRLPEPERIRAAIQAHFAAGGPLSGRHVIVTAGPTHEPIDPVRVIANRSSGKQGFAV
                     AAALGALGARVTLVAGPVALATPAGVERLDVETAREMEAAVAAALPADAAVLVAAVAD
                     WRVEPSPSKLKKAAGPPDLDWLPNPDILAGLAASPQRPPLLIGFAAETGDVVNVAQAK
                     RATKGCDWIVANDVSGNVMGGDRNRVHLITDAGVESWEEGEKREVARHLADRIAAALG
                     ENDAHGPAASDSTGEAG"
     gene            2750..3208
                     /locus_tag="VGB57_13035"
     CDS             2750..3208
                     /locus_tag="VGB57_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0132317.1"
                     /translation="MRTDLLLLTPLARLADLALLLLRWVTGAFLVYQSHDNVLSAERM
                     AEFEKFLVQFNFPMPGLMAPLCVYAQFLCGIAFVLGLLTRWAGLVTAFVFLVAVWMVH
                     WAQDFVGWWPALILIFIGLLFATRGAGRYSLDHRIERRDDRHRGQKTYQR"
     gene            3171..3608
                     /gene="dut"
                     /locus_tag="VGB57_13040"
     CDS             3171..3608
                     /gene="dut"
                     /locus_tag="VGB57_13040"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY0132318.1"
                     /translation="MIDIEVKRLTNGEGLPLPAYASDGAAGLDVVAAEDLSLEPGKRH
                     AVATGFALAIPPGYEVQVRPRSGLALKHGITCLNTPGTIDSDYRGEVKVILANLGTEP
                     FEVRRGERIAQLVPAPVLRAAFREVEELGATERGAGGFGSTGR"
     gene            3605..4366
                     /locus_tag="VGB57_13045"
     CDS             3605..4366
                     /locus_tag="VGB57_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEY0132319.1"
                     /translation="MTLRDDQLDRYARHIVLKEIGGEGQRRLLESAVTLVGAGGIGSP
                     ALQYLAAAGVGGLKVIDDDIVSLDNLQRQILFGTADVGRPKVEVAGEASRRLNPDVRF
                     RENRERLTARNAEALLAGAAVVIDGSDNFATRLLVSDTCTAMRIPLVSAAVGMFQVQV
                     GTWRGWEPGRPCYRCFVGDAFDSDDCDNCAELGVLGATAGIAGSWAAMEAIRQIVGFG
                     EAASGKVHVFDGLAPSMRTLRIAKDPGCRTCAGLT"
     gene            complement(4524..4820)
                     /locus_tag="VGB57_13050"
     CDS             complement(4524..4820)
                     /locus_tag="VGB57_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132320.1"
                     /translation="MRRSWTLVGLGVSAELLALALWALIRLYEVLFWPFLLLSVVAGL
                     AGAWIVLATMFDLTFHPPRGERLRPVRGFDLVLGSGLFILAFLQLRDALGQLPA"
     gene            complement(4820..>5521)
                     /locus_tag="VGB57_13055"
     CDS             complement(4820..>5521)
                     /locus_tag="VGB57_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEY0132321.1"
                     /translation="KFTRRFGQGGEDGAAEEGPEILGKHPETGEDIVRKTGRFGPYIE
                     MGQGKEAKRASIPKDAGELDLDLAVRLLSLPRTVGAHPESGKPITASIGRYGPYLAHD
                     GKYAKLGSTAEVCETGMNAAVVKLAEAAAGGGRARGSREPLKVLGAHPRTELEIKLME
                     GRFGPYVTDGTTNATLPKSLAPDQLTLEEAAQLIDERAAKGPPAKKGRGRKPAAKKAP
                     AKKAPAKKAPAKKKG"
BASE COUNT          873 a         1853 c         1881 g          914 t
ORIGIN      
        1 ggaggccgcc tcggcttcgg agcttcggga aaacctcgtg gccgagagcg gcttctacgt
       61 ccccgcgatc gactggactc ggacgtcgag gcgggtgctg acccttgaat ggatcgacgg
      121 gatcaagctc accgaccggg atgccctggt cgcggccggg cacgacctca agtcgctcgc
      181 ggcaatcctc gtccgggcct tccttcgcca gtcggtgatc gacggcttct tccacgccga
      241 cctccaccag ggcaacctct tcgccctccc ggacggccgg ctcgcggcga tcgacttcgg
      301 gatcatgggc cggatcgacc gccaggcgcg gatgtggctg gccgagatcc tctacggcct
      361 gctcaccggc aattaccgcc gggtcgccga gatccatttc gaggcgcaat atgtgcccgg
      421 ccatcacgac gtagatgagt tcgcgacggc actgagggcg gtcggcgagc cgatccgcgg
      481 ccttccggtc aaggacatct cgatcggcca gatgctcgac ggcctgttca ccatcacccg
      541 cgacttcgag atgccgaccc agccgcacct cctcctcctc cagaagacga tggtgatgga
      601 ggaaggggtc gcacacgccc tcgatcccga catcaacatg tgggagacgg ccgagccttt
      661 cctgagggaa tgggtgcgag gcgagctcgg gcccgaagcg gcgctggcgg accggatcgt
      721 gcgtgacttc cgcaccttcg cgaagattcc ggagctgatc cgacgcatcg accattatta
      781 tccgccgccc ggagcggctc cgccggaccc gccgctcacc gacgtcatcg tggtgaagcc
      841 ccggcgctat tggcgctatg cgctgacggc aatcggctcg gccgcggctg gcgcggcggc
      901 ggtttggcta ctactttgac gatatgaccc gcggcgacgt cctcgatgcc ctccggccac
      961 tggagcctga actcaagcgg cttggcttcg cgaaactcta cttgttcgga tcggccgccc
     1021 gttccgagcc aaacgcccgc gacgtcgacc ttctttacga ggccgagggt gctcttaagc
     1081 ctggcttcaa cgagcttgcc gatgcgtggg atcggctgga aagtgtactt ggcaaacccg
     1141 tagacctcgt cgacatagag ctgctccatc aacgcattcg cccccgcgtc gagaacgaga
     1201 tggtcgaggt ttactgatga acgcagggcc agaagcggac gccctttacc tcgatcagat
     1261 agtcgagctg attcaactta tccgggagag cgtcgtgggc accgacgagg ccgagttcct
     1321 tctcgaccga acgaaagggg acgcaacggc actccgcctt ggcgccatcg gcgaggccag
     1381 ccgtaagctt tcggaagaat tgcaggaacg gcatcctgaa atagaatggc gcaggatgta
     1441 cggactccgt aacatcgtcg cacaccacta tcgcaagcta aactatcgca ttctttggca
     1501 gatcgcgacc agcacgctgg atccgctaga gaacgcctgc cgcgatgagc tttcgcgctt
     1561 cgacggatga acggcaatcg catccttctc atcgtcggcg gcgggatcgc ggcttacaaa
     1621 gcgtgcgagc tcatccggct gatccgcaag gcgagcggca gcgtgcgctg cgtgcttacc
     1681 gccggaggcg ctcagttcgt gactccgacg acgctcgccg ccttgtccga gcagcccgtc
     1741 cacaccagcc tgtgggacct caaggacgag gtcgagatgg ggcatatcca gctcagccgg
     1801 gaggcggacc tggtggtcgt ctgcccggcc acggccgatt tgatggcgaa gatggcggcg
     1861 gggatcgccg acgatctcgc caccaccctc cttctggcca ccgacaagcc ggtactcgcg
     1921 gctccggcga tgaacgtccg gatgtggcgg cacgaggcga cccagcgcaa cgtcgcgcgg
     1981 ctgcggtcgg acggcgtgac catcctcgag ccggacgaag gcgagatggc gtgcggggaa
     2041 tatggccccg ggcggctgcc ggagccggag cgcatccgcg cggccattca ggcgcacttt
     2101 gccgccggcg gtccgctgtc gggccgccac gtcatcgtca ctgcgggacc gacccacgag
     2161 ccgatcgatc cggtgcgggt gatcgccaac cgctcttcgg gaaagcaggg cttcgccgtc
     2221 gccgcggcac ttggggcgct cggggcgcgg gtgaccctgg tggccgggcc ggtggcgctc
     2281 gccactccgg ccggagtcga gcggctcgac gtggagaccg cccgggagat ggaggcggcc
     2341 gtcgctgcgg cgctcccggc ggacgcggcg gtgctggtgg cggcggtcgc cgactggcgg
     2401 gtcgagccct ccccttccaa gctcaagaag gcggccggcc cgcccgatct agactggctg
     2461 cccaatcccg acatcctggc gggtctggcg gcaagcccgc agcggccgcc gctgctgatc
     2521 ggcttcgcgg ccgagaccgg ggacgtcgtg aatgttgcgc aggcgaaacg agcaaccaag
     2581 gggtgcgact ggatcgtcgc caatgatgta tccgggaacg tgatgggagg cgaccgcaac
     2641 cgggtccacc tgatcaccga cgccggcgtc gaaagctggg aggagggtga gaagagggag
     2701 gttgcacggc atctcgccga caggatcgcc gccgcattgg gggaaaacga tgcgcacgga
     2761 cctgctgctt ctgactccac tggcgaggct ggctgacctc gctctgctcc tgctgcgctg
     2821 ggtgaccggc gccttcctcg tctatcagag ccacgacaat gtgctgagcg cggagcggat
     2881 ggcggagttc gagaaattcc tcgtccagtt caacttcccg atgcccggcc tgatggcgcc
     2941 gctctgcgtc tacgcccagt tcctgtgcgg gatcgccttc gtcctcggcc tgctcacccg
     3001 ctgggccggg ctcgtcaccg ccttcgtctt cctcgtcgcg gtgtggatgg tgcactgggc
     3061 gcaggatttc gtcggctggt ggccggcttt gatcctgatc ttcatcggac tgctgtttgc
     3121 aacccgggga gccgggcgct actcgctcga tcaccggatc gagaggcgcg atgatcgaca
     3181 tagaggtcaa aagacttacc aacggtgagg gcctccccct ccccgcttat gccagcgacg
     3241 gggcggccgg cctcgacgtc gtcgccgccg aggacctgag tctcgagccg ggcaagcgcc
     3301 acgccgtcgc gaccggcttc gccctcgcca tcccccccgg ctacgaggtc caggtccgcc
     3361 cgcgctccgg actggcgctg aagcacggca tcacctgcct caacacgccc ggcacgatcg
     3421 acagcgacta tcgcggcgag gtgaaggtga tcctcgccaa tctcggtacc gaacccttcg
     3481 aggtccggcg cggcgagcgc atcgcccagc tggtgccggc gccggtgctt cgggccgctt
     3541 tccgcgaagt ggaggagctc ggagcgacgg aacgcggcgc cggcggcttc ggctcgacgg
     3601 ggcggtgacg ctccgggacg accagctcga ccgctacgcc cggcacatcg tcctcaagga
     3661 gattggcggc gagggccagc gcaggctgct cgagtcggcg gtaaccctgg tcggcgccgg
     3721 cgggatcggt tctccggccc tccagtatct cgccgccgcg ggagtgggcg ggttgaaggt
     3781 gatcgacgac gacatcgtct cgctcgacaa tctccagcgc cagatcctgt tcgggaccgc
     3841 cgatgtcggc cggcccaagg tagaggttgc cggggaagca tcgagacggc tcaacccgga
     3901 cgtccgcttc agggaaaatc gggagcggct gaccgcccgc aatgccgaag ctctgctggc
     3961 gggcgcagcg gtggtcatcg acggaagcga caatttcgcc acccggcttc tggtctccga
     4021 tacctgcacg gcgatgcgga tcccgctcgt ctccgcggcg gtcggcatgt tccaggtcca
     4081 ggtcggaacc tggcgcgggt gggagcccgg ccgtccctgc tatcgctgct tcgtcggcga
     4141 cgccttcgat tcggacgatt gcgacaattg cgccgagctt ggcgtgctcg gagccacggc
     4201 ggggatcgcg ggcagctggg cggcgatgga ggcgatccgg cagatcgtcg gtttcggcga
     4261 ggcggcgtcc ggcaaggtcc atgtcttcga cgggctggcg ccgtcgatgc ggaccctgag
     4321 gatcgccaag gacccaggct gccggacctg cgccggcctg acctaaatcc tccccggcat
     4381 ttgccgggga gggggaccat gggaagcatg gtggaggggc tctttttcct actccgaaga
     4441 acccctcccc cgcgcttcgc gcggtccccc tcccccggaa agccggggga ggatttaaaa
     4501 gggccatcca gcaaggcgct cgatcacgcc gggagttggc cgagcgcgtc gcgcaattgc
     4561 aggaaggcga ggatgaacag cccgctcccg agcacaaggt cgaagcctcg caccggccga
     4621 agccgctcgc cgcgcggggg gtggaaggtg aggtcgaaca tcgtcgcaag aacgatccag
     4681 gcgccggcca ggccggcgac gaccgagagc agcaggaagg gccagaacag gacttcgtag
     4741 agcctgatca aggcccacag agccaaggcc agcagctcgg cgctgactcc gagcccgacg
     4801 agggtccagc tccgccgcat cagcccttct tcttggcggg cgccttcttc gccggcgcct
     4861 tcttcgccgg tgccttcttg gcggcgggct tgcggcccct tcccttcttg gccggagggc
     4921 ccttggcggc gcgctcgtcg atcagctggg cggcttcctc gagcgtgagc tggtcgggcg
     4981 cgagcgactt gggcagggtg gcgttggtgg tgccgtcggt gacatagggg ccgaaccggc
     5041 cctccatcag cttgatctcg agctcggtgc gggggtgggc gcccagcacc ttgagcggct
     5101 ctcgcgagcc cctcgcccgt ccgcccccag ccgccgcttc ggcgagcttg acgacggccg
     5161 cgttcattcc cgtctcgcac acctcggcgg tcgagccgag cttcgcatat ttgccgtcat
     5221 gggcgagata ggggccgtag cggccgatcg aggcggtaat cggcttgccg ctttccggat
     5281 gcgcgccgac ggtgcgcgga agcgacagca gccggaccgc aaggtcgagg tcgagctcgc
     5341 cggcatcctt ggggatggac gcccgcttcg cctccttgcc ctgccccatc tcgatatagg
     5401 gaccgaagcg gccggtcttg cggacgatgt cctcgccggt ctcgggatgc ttgccgagta
     5461 tctccggccc ctcctccgcg gcgccgtcct cgccgccctg gccgaagcgg cgggtgaact
     5521 t
//
LOCUS       DASWNU010000650         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_76766, whole genome shotgun sequence.
ACCESSION   DASWNU010000650 DASWNU010000000
VERSION     DASWNU010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76766"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1908)
                     /gene="sppA"
                     /locus_tag="VGB57_13060"
     CDS             complement(37..1908)
                     /gene="sppA"
                     /locus_tag="VGB57_13060"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0132322.1"
                     /translation="MKFAGKVWKLLVGIKDGLVLLFMLLFFALLYAAMTARPTLGAGD
                     RGALLLQLDGPIVEQPAQATASEVFSGGGPREYRLRDVVHSLRAAAEDDRVKAVALDL
                     DIFSGGGQATLSSVGEALDAVRKANKPVLAYATAYDDDSYLLAAHASEVWVNPMGGVL
                     VTGPGGANLYMKGLLDKLGVTANVYKAGTYKAATEPLTRTDMSPEARQALSAVYGVMW
                     ESWQQEVRQARPKAQLAPYVADLPGRVAAANGDMARAALQAGMVDRVGEREAFDRRVA
                     EIAGKRFAELPGSFERIDYRKWIEANPASDSGGQIGIVTVAGDIVDGEANLGTAGAET
                     IVRALEKGLRERDLKALVVRIDSGGGSALASERIRQAVLAAKAKGLPVVASMGSVAAS
                     GGYWVAMAGDRIYAEPSTITGSIGVFGVLPSFEGAMTKLGLGADGVKTTPLSGEPDLL
                     RGPSPEADRLLQMGVDGTYRRFLALVSGARKIPVAQVDQIAQGRIWAGGTAHQLRLVD
                     SFGGLEDAVREAARLAKLDPEEAKAVWLEKEPGFADQLLMSMAGDGEEEASPDAFSRL
                     AAKPRLRMLAAIGEAERLVSGAAIQARCLECAGHGARPRAPLGGSGVTGWLWRLLGA"
     gene            2141..2428
                     /gene="groES"
                     /locus_tag="VGB57_13065"
     CDS             2141..2428
                     /gene="groES"
                     /locus_tag="VGB57_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0132323.1"
                     /translation="MKFRPLHDRVLVRRVEAEEKTAGGIIIPDTAKEKPQEGEVVAAG
                     SGTRGEDGTITPLDVKTGDKILFGKWSGTEVRLDGEDLLIMKESDILGIVG"
     gene            2537..4186
                     /gene="groL"
                     /locus_tag="VGB57_13070"
     CDS             2537..4186
                     /gene="groL"
                     /locus_tag="VGB57_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049566.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0132324.1"
                     /translation="MAAKDVRFSRDARERIMRGVDILADAVKVTLGPKGRNVVLDKSF
                     GSPRITKDGVTVAKEIELKDKFENMGAQMLREVASKTNDLAGDGTTTATVLAQSIVRE
                     GMKSVAAGMNPMDLKRGIDLAVIRVVEDLKARSKPVAGTNEIAQVGIISANGDTVVGE
                     KIAEAMDKVGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFITNPEKMQVELSEP
                     YILIHEKKLSNLQAMLPILEAVVQSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTNGEMISEDLGIKLENVTVAMLGQAKRVTIDKDNTTIVD
                     GAGEGDRIKARVEAIRAQIENTTSDYDKEKLQERLAKLAGGVAVIKVGGSSEIEVKER
                     KDRVDDALHATRAAVEEGIVPGGGTALLYATKALQDMKGANDDQTRGIDIVRRALQSP
                     IRQIAENAGHDGAVVAGKLIDGEDQTMGFNAQTEVYENLVAAGVIDPTKVVRTALQDA
                     ASVAGLLITTEAAVSEAPEDKPAGGGGMPGGGMGGMGGMDF"
BASE COUNT          804 a         1562 c         1481 g          654 t
ORIGIN      
        1 tggccgcgga gtcgtcgggc gtcgccgccc tcggcctcat gcgccgagca ggcgccagag
       61 ccagccggtc acgccggaac cccccagcgg cgcccgcggg cgcgcgccat ggccggcgca
      121 ttcgaggcat cgcgcctgga tggcggcgcc cgaaaccagc cgttcggcct cgccgatcgc
      181 cgcgagcatc ctgaggcgcg gcttggcggc aagccggctg aacgcgtccg gcgaggcttc
      241 ctcctcccca tcgccggcca tggacatcaa aagctggtcg gcgaagccgg gctccttttc
      301 gagccatacc gccttggcct cctcgggatc gagcttcgcc agccgcgccg cctcgcgaac
      361 ggcgtcctcg aggccgccga acgagtcgac gaggcggagc tggtgggccg tgcctccggc
      421 ccagatccgt ccctgggcga tctggtcgac ctgggcgacc gggatcttcc tggctccgga
      481 gaccaaagcg aggaagcgcc ggtaggtgcc gtcgacgccc atctggagca gccggtcggc
      541 ctcgggcgag gggccgcgga gcaggtccgg ctcgcccgac aggggcgtcg tcttgacgcc
      601 gtcggcgccg aggccgagct tggtcatcgc gccctcgaag ctgggaagca ccccgaacac
      661 tccgatcgag ccggtgatcg tggagggctc ggcatagatc ctgtcgcccg ccatcgccac
      721 ccaatagccg cccgacgcgg cgacgctccc catcgacgcg accaccggca gccccttcgc
      781 cttggcggcg agcaccgcct ggcgaatgcg ctcggaggcg agggcggagc cgccgccgga
      841 atcgatccgg acgaccagcg ccttgaggtc gcgctcgcgc aggcccttct ccagcgcccg
      901 gacgatcgtc tcggcgccgg cggtgccgag attggcctcg ccgtcgacga tgtcgcccgc
      961 gaccgtgacg atcccgatct ggccgccgct gtcgcttgcc ggattggcct cgatccactt
     1021 gcgatagtcg atccgctcga agcttccggg aagctcggcg aagcgctttc cggcgatctc
     1081 ggcgacccgg cggtcgaacg cctcgcgctc gccgaccctg tcgaccattc cggcctgcag
     1141 cgcggcccgg gccatgtcgc cattggccgc ggcgacccgg cccggaagat ccgcgacata
     1201 gggggccagc tgcgccttcg gacgcgcctg gcgcacctcc tgctgccagc tttcccacat
     1261 gaccccatag accgcgctca gcgcctgcct cgcctcgggc gacatgtcgg ttcgggtgag
     1321 cggctctgtc gccgccttgt aggtgccggc cttgtagacg ttggcggtca ccccgagctt
     1381 gtcgagcagg cccttcatgt acaggttggc gccgcccgga ccggtgacca gcactccgcc
     1441 catcggattg acccagacct cgcttgcatg ggcggcgaga aggtagctgt cgtcgtcata
     1501 ggcggtggcg taagcgagga cgggcttatt ggccttcctg acggcgtcga gcgcctcgcc
     1561 gaccgacgac agggtcgcct ggcctccgcc ggagaagatg tcgaggtcga gcgccaccgc
     1621 cttcaccctg tcgtcttccg cggcggcgcg aagcgagtgg acgacgtcgc gaagccgata
     1681 ttcgcgcggg ccgccgccgc tgaacacctc gctcgcggtg gcctgggcgg gctgctcgac
     1741 gatggggccg tcgagctgga gcaggagcgc tccgcggtcg ccggcgccga gcgttgggcg
     1801 agccgtcatc gccgcgtaga gaagcgcgaa gaagagcagc atgaacagga ggacgagccc
     1861 gtccttgatc ccgacgagca gtttccagac cttgcccgcg aacttcattg catcactccc
     1921 ggcttctcgt tcccaagggc tagaccagcc cccgcttcct gtaaatcggc gcatccgccc
     1981 ccgcaaaatt cagtgggtca gacccttgac ctgcgatgaa caagccctag atcggcaacg
     2041 ctggcactcg cgagggctga gtgccaacag tttcaaccag gacggccgcc tcacccaata
     2101 tggggacggc agccgcaata gcgaagaaga ggtgacgcgc atgaaatttc gtccgctcca
     2161 tgacagggtg ctcgtccgcc gggtcgaggc cgaggagaag acggccggcg gcatcatcat
     2221 ccccgacacc gccaaggaaa agccccagga gggcgaggtc gtcgccgccg gctccggcac
     2281 gcgcggcgag gacggcacga tcacgccgct cgacgtcaag accggcgaca agatcctgtt
     2341 cggcaaatgg tccggcaccg aggtccgcct cgacggcgag gacctgctga tcatgaagga
     2401 aagcgacatc ctggggatcg tgggctgagg ccagccgacg atgcccatcc cggcgaaggc
     2461 cgggatccca gttcgattta ggcacaacat ctcaacgaga ccccggcttt cgccggggtg
     2521 acgaaaggaa ataaacatgg cagcgaaaga cgttcgcttc tcccgcgacg cccgcgagcg
     2581 catcatgcgc ggcgtcgaca tcctcgccga cgcggtgaag gtgaccctcg gccccaaggg
     2641 ccgcaacgtc gtcctcgaca agagcttcgg aagcccgcgc atcaccaagg acggcgtcac
     2701 cgtcgccaag gagatcgagc ttaaggacaa gttcgagaac atgggcgcgc agatgcttcg
     2761 cgaggtcgcg tcgaagacca acgacctcgc cggcgacggc accaccaccg ccacggtgct
     2821 ggcccagtcg atcgtccgcg agggcatgaa gtccgtcgcg gccggaatga acccgatgga
     2881 cctgaagcgg ggcatcgacc tcgccgtcat ccgcgtcgtc gaggacctca aggcccgctc
     2941 caagccggtc gccggcacca acgagatcgc ccaggtcggg atcatctccg ccaacggcga
     3001 caccgtcgtc ggcgagaaga tcgccgaggc gatggacaag gtcggcaagg aaggcgtgat
     3061 caccgtcgag gaggccaagg gcctcgagtt cgagctcgac gtcgtcgaag gcatgcagtt
     3121 cgaccgcggc tacctgtcgc cttatttcat caccaatccg gagaagatgc aggtcgagct
     3181 cagcgagccc tacatcctca tccacgagaa gaagctctcc aacctccagg cgatgctccc
     3241 gatcctcgag gcggtggtcc agtcgggccg tccgctcctc atcatcgccg aggacatcga
     3301 gggcgaggcg ctcgccacgc tcgtcgtcaa caagcttcgc ggcggcctga aggtcgcggc
     3361 cgtcaaggct ccgggcttcg gcgatcgccg caaggcgatg ctcgaggaca tcgcgatcct
     3421 gaccaatggc gagatgatct ccgaggacct cggcatcaag ctcgagaacg tcaccgtcgc
     3481 gatgctcggc caggccaagc gcgtcaccat cgacaaggac aacacgacca tcgtcgacgg
     3541 tgccggcgag ggcgaccgga tcaaggcccg cgtggaagcg atccgtgcgc agatcgagaa
     3601 cacgaccagc gactatgaca aggagaagct ccaggagcgc ctcgccaagc tcgccggcgg
     3661 cgttgcagtg atcaaggtcg gcggctcgtc cgagatcgag gtcaaggagc gcaaggaccg
     3721 ggtcgacgac gccctccacg ccacccgcgc cgcggtcgag gaaggaatcg ttcccggcgg
     3781 cggaaccgcc ctcctctacg ccaccaaggc gcttcaggac atgaagggcg cgaacgacga
     3841 ccagacccgc ggaatcgaca tcgtccgccg cgcgctccag tcgccgatcc gccagatcgc
     3901 cgagaatgcg ggccatgacg gcgctgtcgt cgccggcaag ctgatcgacg gcgaagacca
     3961 gacgatgggc ttcaacgccc agaccgaggt ctacgaaaac ctggtcgcgg ccggcgtgat
     4021 cgacccgacc aaggtcgtcc gaacggcgct tcaggacgcg gcttcggtcg ccggtctcct
     4081 catcaccacc gaagcggcgg tgagcgaggc tccggaagac aagccggccg gcggcggcgg
     4141 aatgcccggc ggcggaatgg gcggaatggg cggaatggac ttctaagttc cgacgtctgg
     4201 acgctgaaat gaaatgagaa gggccgggga ggcgactccc cggcctttcg tcattgcgag
     4261 cgaagcgaag caatccagag ctgagcccgt cgcctaagct gggctggatt gcttcgtcgc
     4321 ttcgctcctc gcaatgacga aagggatatc ctcgccctca accttccctg tccgcctgcg
     4381 gacggccgtg ttcgcctgcg gtcaaaatac gcctccctcc ggcgtcctgt tcggacctgc
     4441 cgaggggccc ggaccggcac gcttcttgct cctttccggc tgaatacttc agcctcgaaa
     4501 g
//
LOCUS       DASWNU010000651         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_387686, whole genome shotgun sequence.
ACCESSION   DASWNU010000651 DASWNU010000000
VERSION     DASWNU010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387686"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1055)
                     /locus_tag="VGB57_13075"
     CDS             complement(12..1055)
                     /locus_tag="VGB57_13075"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0132325.1"
                     /translation="MTPDVITRIEGKVGRIRLNRPKAIHALTRDMCSAMISALLPWRE
                     DPAVQAVMIDHAEGRGFCAGGDVVMLARSGAGDGAEARAFFHEEYRLNHLLFTYAKPT
                     VAFMDGITMGGGVGISQPCRYRVATENTRFAMPETAIGLFPDVGGGWYLPRLPGRTGQ
                     FLALTGARLDGAECFALGLATHYMQSLALDDLKARILGNPERIETLLDENSTPPPPAR
                     IEANLPLIDRLFASDDYEEILAALEEDGSDWAQKELETLRSKSPQACKVSLRLLYDGA
                     RVQDFGHEMRQEYAVATRVVQRHDFVEGVRALLVDKDNSPRWEPATPEGVSEHLIDTI
                     FAPMPEGEEWTPL"
     gene            complement(1168..2310)
                     /locus_tag="VGB57_13080"
     CDS             complement(1168..2310)
                     /locus_tag="VGB57_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0132326.1"
                     /translation="MSQFDLTDEQRQIQEMARQFTADAITPHAAEWDEKHIFPRDTIR
                     QAAELGFGSIYVSEESGGIGLGRLEAALIMEAMAYGDPSTSAFISIHNMASWMIDRFG
                     SAAVKSKYLPSMIPMERMGSYCLTEPSSGSDAAALKTKAVKDGDCYVVSGAKAFISGG
                     GENEVYVTMVRTGVEGPKGISCLVIEKDMPGVSFGAQERKLGWHSQPTAQVNFDEVRV
                     PAENLVGGEGEGFRIAMMGLDGGRLNIGACSLGGAQRCLDEAIAYTKERKQFGNAIAD
                     FQATQFTLADMETELQAARMLLYAAAVKVTENAPDKSRFAAMAKRLATDTGSSVVDRA
                     LQLHGGYGYLMDYPIERFWRDLRVHSILEGTNQIMRVIVARDMLRQ"
     gene            complement(2350..>2748)
                     /locus_tag="VGB57_13085"
     CDS             complement(2350..>2748)
                     /locus_tag="VGB57_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0132327.1"
                     /translation="KGGKHMNSKALRELYVETMVGAANFYDGLARRTLGRSPAHMLLL
                     HETDLAALYLPALIDALRKDGWEIITADEAYADPIARALPDTPSAQGTRTEAMAWEKG
                     LPAPRWYERENVKIANALFAERVLKETPGP"
BASE COUNT          419 a          900 c          953 g          476 t
ORIGIN      
        1 gggatgacgg gtcaaagcgg cgtccactcc tcgccttccg gcatcggcgc gaagatggtg
       61 tcgatcagat gctccgacac gccttcgggc gtcgccggct cccagcgcgg gctgttgtcc
      121 ttatcgacca gcaaggcccg aaccccctcg acgaaatcgt ggcgctgcac cacccgggtg
      181 gcgacggcat attcctggcg catctcatgg ccgaaatcct gaacccgggc gccgtcgtag
      241 aggaggcgga gcgagacctt gcaggcttgg gggctcttgg agcgcaaggt ctccagctcc
      301 ttttgcgccc agtcggaccc gtcctcctcc agggcggcga ggatctcctc ataatcgtcc
      361 gaggcgaaca ggcggtcgat cagcggcagg ttcgcctcga tccgggcggg gggagggggg
      421 gtgctgttct cgtcgagcag cgtctcgatg cgctccggat tgccgaggat ccgcgccttg
      481 aggtcgtcga gcgcgagcga ttgcatataa tgggtggcga ggcccagagc gaagcattcg
      541 gccccgtcca gccgggcccc cgtcagtgcc aggaactggc cggttcggcc cggaaggcgg
      601 ggcaggtacc agccgccgcc gacgtccggg aacaggccga ttgccgtctc gggcatggcg
      661 aagcgggtat tctcggtggc gacacggtag cggcagggct gcgagatgcc cactccgccg
      721 cccatggtga tcccgtccat gaaggcgacg gtcggcttgg cgtaggtgaa gaggaggtgg
      781 ttgagccgat attcctcatg gaagaaggcc cgcgcctccg ccccgtcgcc ggccccgctt
      841 cgggccagca tcaccacgtc gccgccggcg cagaagccgc ggccctcggc atggtcgatc
      901 atcaccgcct gaacggcggg gtcttcgcgc cagggcagca gggcggagat catcgcgctg
      961 cacatgtcgc gggtgagggc gtggatcgcc ttggggcgat tgaggcggat ccggccgacc
     1021 ttgccttcga ttcgggtgat aacgtccggg gtcattcgat ctcgtcatcc cggcgaaggc
     1081 cgggatccat ggacaccgaa gtgctcaact tgccatcgac tgtgttcatg ggtcccggcc
     1141 ttcgccggga tgacgcgccg cgcgtaatca ctgcctcagc atgtcccgag cgacgatcac
     1201 ccgcatgatc tggttggtgc cctcgaggat cgaatggacc ctcaggtcgc gccagaagcg
     1261 ctcgatcgga tagtccatca gatagccgta gccgccgtgg agctggaggg cgcggtcgac
     1321 gaccgaggag ccggtgtcgg tggcgaggcg cttggccatg gcggcgaagc gcgacttgtc
     1381 gggggcgttc tcggtcacct tcaccgccgc ggcgtagagc agcatccggg ccgcctggag
     1441 ctcggtctcc atgtcggcga gggtgaactg ggtggcctgg aagtcggcga tggcgttgcc
     1501 gaactgcttg cgctccttgg tgtaggcgat cgcctcgtcg aggcagcgct gcgctccccc
     1561 cagcgagcag gcgccgatgt tgagccggcc gccgtcgagc cccatcatcg cgatccggaa
     1621 gccctcgccc tcgccgccga ccagattctc ggccggaacc ctcacctcgt cgaaattgac
     1681 ctgggcggtc ggctgcgaat gccagccgag cttgcgctcc tgggcgccga agctgactcc
     1741 cggcatgtcc ttctcgatca ccaggcagga gattcccttg ggcccctcga ccccggtacg
     1801 gaccatggtc acgtagacct cgttctcgcc gccgccggag atgaaggcct tggcgcccga
     1861 gacgacgtag caatcgccgt ccttcaccgc cttggtcttg agcgccgccg cgtccgaccc
     1921 ggagctcggc tcggtcaggc aatagctgcc catccgctcc atcgggatca tcgagggcag
     1981 gtatttcgac ttcaccgccg ccgacccgaa gcggtcgatc atccagctcg ccatgttgtg
     2041 gatcgagatg aaggccgagg tggagggatc gccataggcc atcgcctcca tgatcagggc
     2101 cgcctcgagc cgcccgagcc cgatcccgcc gctctcctcg gagacgtaga tcgacccgaa
     2161 gccgagctcc gccgcctgcc ggatcgtgtc gcgcgggaag atgtgcttct cgtcccattc
     2221 ggcggcgtgg ggcgtgatcg cgtcggcggt gaattggcgc gccatctcct ggatctggcg
     2281 ctgctcgtcg gtaaggtcga actggctcat gcgcgcgctc tagcgaacgc ggcgggaatc
     2341 gcaatcggat caggggccgg gcgtttcctt cagcacccgc tcggcgaaca gggcgttggc
     2401 gatcttcaca ttctcgcgct cgtaccagcg cggggccggc aggccctttt cccaggccat
     2461 ggcctcggtc cgggttccct gggcggaagg cgtgtcggga agggcgcggg cgatcggatc
     2521 ggcataggcc tcgtcggcgg tgatgatctc ccacccgtcc ttgcggagcg cgtcgatcag
     2581 ggcggggagg tacaaagcgg cgaggtcggt ctcgtggagc aggagcatgt gcgccggcga
     2641 acggccgagg gtgcggcggg cgaggccgtc gtagaaattg gccgccccga ccatcgtctc
     2701 gacgtagagc tctcggagcg ccttcgagtt catatgcttg ccgccctt
//
LOCUS       DASWNU010000652         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_51359, whole genome shotgun sequence.
ACCESSION   DASWNU010000652 DASWNU010000000
VERSION     DASWNU010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51359"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VGB57_13090"
     CDS             <1..609
                     /locus_tag="VGB57_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY0132328.1"
                     /translation="ARLASRPPGEAILIAVSKTHPPESIRPLIEAGQREFGENRVQEA
                     QDKWPALKAETPGLRLHLIGRLQSNKAADAVALFDSIHSVDRPSLVSALAEAMAREGR
                     RPDCFLQVNIGDEPQKGGCPVADLPALIEASREAGLPVAGLMCIPPADVEPAPYFALL
                     AKLAGRHGLAGLSMGMSADYETAVATGATHVRVGTALFGDRS"
     gene            624..1283
                     /locus_tag="VGB57_13095"
     CDS             624..1283
                     /locus_tag="VGB57_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0132329.1"
                     /translation="MFDFDGVIVDSEILSNTILAEALTDLGHPTSLEQAVERYIGLNW
                     RDMCVVIEAQIGRPLPTDFRTRSKEAFELRLEEVTAVAGVEAFLAALPPIPKAVASSS
                     PTQWLRGSLQRFGLAQYFGDRLFSAAEHVERGKPHPDIYLHAARALGVRPGEVLVLED
                     TPTGVKAARAAGMTVVGLCAGLHCGPGHGERLRSAGADHIAGSYPEVLKLVMPDLIRH
                     P"
     gene            complement(1365..>1728)
                     /gene="ribA"
                     /locus_tag="VGB57_13100"
     CDS             complement(1365..>1728)
                     /gene="ribA"
                     /locus_tag="VGB57_13100"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HEY0132330.1"
                     /translation="IAAIAASGWGILLYLRQEGRGIGLVNKLRAYALQDQGFDTVDAN
                     LRLGFADDERHYTLAARMLGALAQDEVRLLTNNPRKVEGLEAAGIRVSERVPLRAGGN
                     AHNRDYLETKRSRSGHKL"
BASE COUNT          260 a          604 c          574 g          290 t
ORIGIN      
        1 gcccgcctgg cctcgcgtcc gcccggcgag gcgatcctga tcgcggtctc gaagacccat
       61 cctcccgagt cgatccggcc gctgatcgag gcggggcagc gtgaattcgg cgagaatcgg
      121 gtccaggagg cgcaggacaa gtggccggcg ctgaaggcgg agacgcccgg ccttcggctc
      181 cacctgatcg gtcggctgca gtcgaacaag gcggccgacg cggtggccct gttcgactcc
      241 atccattcgg tcgaccggcc gtccctcgtc tcggcgctgg ccgaggcgat ggccagggag
      301 ggccggcggc cggattgctt cctccaggtc aatatcggcg atgagccaca aaaaggcggc
      361 tgcccggtcg cggacctgcc cgctttgatc gaggcgagcc gcgaggccgg cctgcccgtc
      421 gcgggcctga tgtgcattcc tccggcggac gtcgagccgg cgccttattt cgcgctgctg
      481 gccaagctgg ccggccggca cgggctcgcc ggcctcagca tgggcatgtc ggccgattac
      541 gaaaccgccg tcgcgaccgg ggccacccat gtccgggtcg gcaccgctct gttcggagac
      601 agaagctgaa tctcgaagcc ctgatcttcg atttcgacgg agtcatcgtc gacagcgaga
      661 tcctctccaa caccatcctc gccgaagcgc tgacggatct cggccatccc acctcgctgg
      721 agcaggcggt cgaacgctat atcggcctca actggcggga catgtgcgtg gtcatcgagg
      781 cgcagatcgg gcggccgctc cccaccgact tccggacccg ttccaaggag gcattcgagc
      841 ttcggctgga agaagtcacc gccgtggccg gagtcgaggc gttcctcgcc gcgcttccgc
      901 cgatcccgaa ggccgtggcc tcgtccagcc cgacccagtg gctgcgcggt tcgctccagc
      961 gcttcgggct cgcccaatat ttcggcgacc ggctgttcag cgccgccgag catgtcgagc
     1021 gcggcaagcc ccaccccgac atctacctgc acgcggctcg ggcgctgggg gtccggccgg
     1081 gggaggtcct cgtcctcgag gacactccga ccggcgtgaa agcggcccgg gcggcgggca
     1141 tgacggtcgt cggcctctgc gcgggccttc actgcggtcc gggtcacggc gaaaggctcc
     1201 gctccgccgg ggccgaccat atcgccggca gctacccgga agtgctcaag ctcgtcatgc
     1261 cggacttgat ccggcatcca tgaacacggc ctaaagccgg atttcctccg gcggtgttca
     1321 tggattccgg ctttcgccgg aatgacggat tcggcgccat gacgtcagag cttgtggccg
     1381 ctgcgcgatc gcttggtttc cagataatct cggttgtggg cgttgccgcc ggccctcagc
     1441 ggcacccgct cgctcaccct tatgccggcc gcctccagac cttcgacctt gcgcggattg
     1501 ttggtgagca gccgaacttc atcctgggcg agcgccccga gcattcgggc ggcaagcgta
     1561 tagtgacgct catcgtccgc gaagcccagg cgaagattgg cgtcgaccgt gtcgaagccc
     1621 tggtcctgga gcgcgtaggc gcgcagcttg ttgaccagcc cgatgccgcg gccctcctgc
     1681 cgcaaataaa gcaggatgcc ccagccgctc gcggcgatcg cagcgatc
//
LOCUS       DASWNU010000653        15866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_129274, whole genome shotgun sequence.
ACCESSION   DASWNU010000653 DASWNU010000000
VERSION     DASWNU010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 15866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15866
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129274"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1305
                     /locus_tag="VGB57_13105"
     CDS             436..1305
                     /locus_tag="VGB57_13105"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEY0132331.1"
                     /translation="MKRLPLVLAAPLAASLAAAAPRADVAEVVAAERAFASAARTDGA
                     NAAFRHYSAPDALVFQPGPKLARAALEANPIPPIPLDWWPAYAGIAASGDLGFTTGPY
                     VAGSGERKAHGWYFTVWRRQPDGSWRWIIDHGPPTREAAPEGSGAKVAALPAGRRSPA
                     SKAYGEVKSAEARLAAALAADARAALPRFLAADGRLMRVGPQPAVGAAAWAPLLAAGP
                     ERIDTAPLGGSASAAGDLAFTYGTARWRKGETGVEGHYVRIWQRRREGWKLIVDNMIA
                     IPPPPPARPSAGG"
     gene            1401..2495
                     /locus_tag="VGB57_13110"
     CDS             1401..2495
                     /locus_tag="VGB57_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0132332.1"
                     /translation="MAKIMPRPNVAFQAAKLTLGVWIFTFILFLMPVAVSMGELPRFA
                     VGMTALVVAIGMALTVPLYWVCARLQDSPTALKIGGVFGAVCVTAFVFSLSDALFGAN
                     IIQMFMGEHRIPPDVINRTVSNFISFSWLYGWLGTTYVILLTNAAMRERDLQLAEART
                     LAQTAQLTALRLQLNPHFLFNTLNAISSLIVTGRNRDGEAMLAKLCAFLRTALVGDGR
                     SEVTLGEELETLQTYLEIESIRFGDRLTVEFAAPEALLEYPVPNFILQPLVENAVKHG
                     VAPTSRPVIVRVGARRDGDDLILSVSDNGGAVAGSRVAGTGVGLDNTRRRLEVIYGPR
                     GRVETLAHDDGFTVLLRVPVNQAPALDLVA"
     gene            2492..3244
                     /locus_tag="VGB57_13115"
     CDS             2492..3244
                     /locus_tag="VGB57_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0132333.1"
                     /translation="MTLRTLLVDDEPLALERLQVFFRDIAGAEVIGTARDGREAAEAI
                     ERLKPDLALLDIQMPEQNGLRVAATLPPEDRPEIIFVTAFEQYAPDAFEVEAADYLLK
                     PVRFDRLWQAVERAKRRKHQRTLAGRAEAAASPRDHGREDGLWVPTRAGAVFVPVEAI
                     EWIEAQRDYVMLHTATRSHILRISMRALEDRLDPAQLMRVHRSAFARPANVVEVQRLG
                     RTINALVLRDGAIVQVGPNYSRAVAERLKVAE"
     gene            3460..3822
                     /locus_tag="VGB57_13120"
     CDS             3460..3822
                     /locus_tag="VGB57_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0132334.1"
                     /translation="MPGPMILLVEDEPLVLRNTADLLIEDGYDVAECTGCEEALARLE
                     AEPDTAVLVTDLNVACPGDGMTLAKVAAERWPHLRIVMVSGALRPPQGSYPERAIFFT
                     KPYAPGALLHVVKSPDFA"
     gene            3966..4721
                     /locus_tag="VGB57_13125"
     CDS             3966..4721
                     /locus_tag="VGB57_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0132335.1"
                     /translation="MDDDLPEAIPAATLILMRPAAAGGPPEILMLERAETMAFAAGAL
                     VFPGGRIDPGDEAAANRFPRLPDAAARIAAIRETIEETGVAPALRPAPDAIALREAMA
                     AGRPFAALLDEMNLSLDLEALTPFARWCPNFREARRFDTLFYLAEAPAGRGTRHSADE
                     TESVHTFWASAATVLAEIEAGRAKAIFPTRRNLERLARFGSLGEARADAERHPVRQIT
                     PWVEERGGKRFVCIPEGIGYPVTAEPYESARRR"
     gene            complement(4840..5841)
                     /locus_tag="VGB57_13130"
     CDS             complement(4840..5841)
                     /locus_tag="VGB57_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEY0132336.1"
                     /translation="MTASRLASRQVEKLWGRAELPRSFGAAAASGGEPIGEIWFEDGR
                     GPEAELLVKYLLTREKLSIQVHPDGEAARAAGHRRGKDEAWLVLEAEPGARIGLGLRQ
                     EVSPAGLRAAIEAGTLEALVDWREAKAGDFYYSPAGTIHALGPGLTLIEVQENVDLTY
                     RLFDYGRERELQVEAGVKAARPQPYVSPTPRREIEPGREILAEGGAFVVERWTRNAGG
                     GLAGEVERPVWLVPVKGSGLIDGQPLEPASVWLVEDQSRVRLDPGAEVLVAYPGPSVI
                     ETLFVDTRRSQPVEPIEERRRYRPPVRLEPSFRRLRRGDEISPRLPELAESRSFILP"
     gene            5896..6621
                     /locus_tag="VGB57_13135"
     CDS             5896..6621
                     /locus_tag="VGB57_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY0132337.1"
                     /translation="MRYLRGVKIFLVLALLAFLAFLGWGFARNHPEDVPWTELDLTRP
                     VGVFTGRKLAALSGEGEKCRALLRRAGVRFVALPPRGRAQCGYDDAVRFSSGGALTIG
                     YRPADLGTSCPVAAGLALWEWHVVQPAALRHFGTKVAAIEHFGSYSCRRIYGRPEGAW
                     SEHASANAVDIAAFRLSDGRRIRIVADWEGGGPKARFLREVRDGACRLFATTLSPDYN
                     AAHRDHFHLDQAERGAMGWRGCR"
     gene            complement(6834..7514)
                     /locus_tag="VGB57_13140"
     CDS             complement(6834..7514)
                     /locus_tag="VGB57_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY0132338.1"
                     /translation="MSSRIILLAKRERFSREAQAIAEAAFGADLATYTGAVGDPEPEA
                     LQTGSPEYLISFLSPWIVRKHSLDRAGAAINFHPGSTDYPGIGCYNFALYEGAAEFGA
                     VCHHMLAKVDTGDLIMERRFPVTPDDSVETLKLRTMETMVDMFREIVALIAAGRPLPR
                     AGVHWTRRPFTRREMEALKVIEHDMPKDEIERRVRATVYPGYPGPVMRLADGGTYVFP
                     VPDRPPIA"
     gene            complement(7632..7904)
                     /locus_tag="VGB57_13145"
     CDS             complement(7632..7904)
                     /locus_tag="VGB57_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY0132339.1"
                     /translation="MAKGTSAGGGARGGLSRPVTPSADLAAITGSDPLPRSQVVSKMW
                     DYIRTNKLQNPENKREIVADDKLKKIFGTDRCSMFEMNKHLSRHLT"
     gene            complement(8046..8861)
                     /locus_tag="VGB57_13150"
     CDS             complement(8046..8861)
                     /locus_tag="VGB57_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY0132340.1"
                     /translation="MRIGIVAPSTPILPETAERVKAIAAQAVPGAELVFHPQCFLSHR
                     HFAGDDGARAEAFVEVANDPGFDALWVARGGYGSCRIAEDAVARLGPAARGKTYLGYS
                     DAGYVLAALYRAGFERLAHGPMPGDVRRDGGETAVARALSWLVRGDPAALEPSLGDGP
                     AAAFNITVFSQLLGTALEPDLAGHVLMLEDVSEHMYRTDRSLFHITGNAGVRRVAGIR
                     LGRCTEVPDNDPDFGASEEEVFLHWCGRSGIPYLGRADIGHDAGNKVVPFGRR"
     gene            8914..10323
                     /locus_tag="VGB57_13155"
     CDS             8914..10323
                     /locus_tag="VGB57_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0132341.1"
                     /translation="MSGTKSYFFCGIGGSGMLPLAAIVRGQGARVSGSDRALDQGRTA
                     AKFDYLRGLGIELYPQDGSGPRAGQILVASAAVEESVPDVQAARRLGLERITRAELLS
                     RLFNAAATGIAVGGTSGKSTVTGMIAWILHRAGLEPTVMNGAVMKNFATSDAPFASAL
                     VGGDRVFVSEVDESDGSIALYRPKIALLNNVSLDHKSMEELRALFGDFLGRAGTAVVN
                     LDDPEARALAGAGAVTFSLADPRADFNASGIVEAPTAVQFRVAERSSGEAAAVRLPVP
                     GRHNVANALAALASARAAGVALAAAAEALSSFAGLRRRFDIVGTRGGVTVIDDFGHNP
                     DKIAATLATCHAFPGRLLLFFQPHGYGPLRTMRKELVAAFAGNMAREDVLILCDPAYF
                     GGTVERTVTSADLVADVAGAGRSAEHIPDRAEAGRRLAALARPGDRILVMGARDDTLS
                     QFAGEILNCLPMLRMGRQI"
     gene            complement(10320..10838)
                     /locus_tag="VGB57_13160"
     CDS             complement(10320..10838)
                     /locus_tag="VGB57_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HEY0132342.1"
                     /translation="MADEEKGGPPAYPPSAVHLARTAASAQMNLSAMADQKASILMGA
                     AFVIFTIAVGHSDGKGAPLPLLILGGAAFLSAVFAVLAILPAVGYRKRAPLNLMFFGS
                     FTRLDEDDYIERLLTELRSDDSIYRTMARDIYQNGIVLARKKYRMLGWAYRIFLAGLA
                     ASLIAFVIEALS"
     gene            10960..11208
                     /locus_tag="VGB57_13165"
     CDS             10960..11208
                     /locus_tag="VGB57_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0132343.1"
                     /translation="MTDREETFAKVVQLIEPFNKKDAELSDATTFAGDLEWDSLIVMD
                     FVASVEDEFDILITMNMQAEIETVGQLVDAVEELRNDG"
     gene            11229..12467
                     /locus_tag="VGB57_13170"
     CDS             11229..12467
                     /locus_tag="VGB57_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0132344.1"
                     /translation="MDPAGEPAGHTHGRDLFSKFDALIAERDTLLDTGVRDPFGLVME
                     EVLSPTRAIVKGKETILLGTYNYMGMTFDPDVIEAGKNALDAFGSGTTGSRVLNGTYQ
                     GHMEVEEALKDFYGTRDAMVFSTGYQANLGLMSTLAGKEDYIILDADSHASIYDGCKL
                     GDAQIVRFRHNNVEDLDKRLGRLPAQGGRLVVLEGVYSMLGDIAPLKEMVEVAKRHGA
                     MVVVDEAHGMGFFGPNGRGVFEEAGVEDQVDFVVGTFSKSVGTVGGFVVSNHPKFGVL
                     RLVCRPYVFTASLPPSVVATAATSIRKLKFAGNKRQHLWENSRRLHKGLRDLGFDLAT
                     PEAQSAIIAILLPDQDRTVRMWQALLEAGVYVNMARPPATPAGMYLLRCSLCADHSEE
                     QVTQILAMFESAGKATGILQ"
     gene            complement(12603..13979)
                     /gene="chrA"
                     /locus_tag="VGB57_13175"
     CDS             complement(12603..13979)
                     /gene="chrA"
                     /locus_tag="VGB57_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY0132345.1"
                     /translation="MSSSASTERPPEPETSVPLAEAVRVWAKIALLSFGGPAGQIALM
                     HRVLVEERRWIGEARFLHALNYCMLLPGPEAQQLATYIGWLMHRTKGGLIAGILFVLP
                     GFAAIMILSWIYAAAGNVGWVAALFFGLKAAVLAIVAEAVRRIGARALGNNVLRGIAA
                     AAFVAIFFFAVPFPLIVLAAGLAGYAGGRSGAGAFSGGGGHGPEPAGKSLLGDRIPAH
                     AVPSLRWALRISAIFLLLWLGPLALLLATAGPDSVFSKIGLFFSQMAVVTFGGAYAVL
                     AYVAQQAVETYGWLAPGEMLDGLGMAETTPGPLIMVTQFVGFMGAYRDPGGLHPMVAG
                     TLGGLLTTWVTFTPCFLWIFLGAPFVEKLRENRALTAALTAITAAVVGVVLNLAIWFA
                     LHTLFRVVREVAAGPLRFDLPVPASVDLAACALAAAALVAMFRFRLGAPVVLAGCAAA
                     GMLWWALG"
     gene            14175..>15866
                     /locus_tag="VGB57_13180"
     CDS             14175..>15866
                     /locus_tag="VGB57_13180"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0132346.1"
                     /translation="MSEERTSGAWRGVLASAGAVLAALLLVALMLMVTSSNEVRDRAL
                     ASERHSYDVMLLTRTVDGTIARAEAALGRYVLDEDVATSGSVYGAEWRQAQQLIAELR
                     RQVRGNKEQLARVEALEKLFATRGRELAPAAVAAQAKAGSGGISLFYGAARTPTGQQL
                     RNTLAQIATAEREDLRRRMAATRVSGTRSDQLTEWLGWLAVAIGLGAIVLGWLAFRAI
                     SGRLAALREADSEANRARQLEEAVRERTRELSQAYEQLKAESAEREAAEAQLRQVQKM
                     EAVGQLTGGIAHDFNNMLAVVVGGLDLARRKLTGPRREVEFHLDNAMEGATRAAALTR
                     RLLAFARAEPLLPVAVAPAGLVEGMLDLIDRSIGERITVRTRFPDRTWNVSVDTNQLE
                     NAILNLAVNGRDAMDGEGQLAIAVENVVLSAGEAGELGAGDYVRISVSDTGSGMPPEV
                     LERVFEPFFTTKPTGKGTGLGLSQIHGFAAQSGGEARMESEVGRGTTLHFLLPRTKKE
                     PPSQAAPQLDIALPLGLRILLVEDSEHVRYFARHLLEDLGCAVVEAADAEQALALL"
BASE COUNT         2537 a         5818 c         5168 g         2343 t
ORIGIN      
        1 aagacacgcc gagcgaatcc cgtcgaaccg ccctcgcgcg ttgggagggg cccggccgag
       61 cctcggcacc cctccccgcg cgctgtgcgg aacttgtccc gtttcaccat gtcaaagagc
      121 cctgccataa gcaacgcggc agcaggaaca gtataggaac aacccgaccg caattctcct
      181 acagcttgat ccgtttcgga agttgcgggg aattccctct ccctgcaggg gagagggaat
      241 gaaggcggca tgttcgtcgc gctgaaacac gatttcgtcg cccccgcgca ccttcgccgc
      301 ttcgatcgga gcggtggtcg cagccgccgc gcccgctccg gacactccgc tatcgaggaa
      361 ccaccgccgc gggagaccgg cacgccccct cgccccctcc cgcggcggac ccttttggga
      421 aatccggatc gttcgatgaa gcgccttcca ctcgtcctcg ccgccccgct cgccgcctcg
      481 ctcgccgccg ccgcgccccg cgccgacgtc gccgaggtcg tcgccgccga gcgggccttc
      541 gcgagcgccg cccggaccga cggggccaat gccgctttcc ggcattattc ggcgcccgac
      601 gctctcgtct tccagccggg cccgaagctc gcgagggccg cgctcgaggc caatcccatc
      661 ccgcccattc cgctcgactg gtggcccgct tatgccggga tcgccgcctc gggcgacctc
      721 ggcttcacca ccggccccta cgtcgccggc tccggcgagc gtaaagccca tggctggtat
      781 ttcacggtct ggcggcgtca gcccgacgga agctggcgct ggatcatcga ccacggcccg
      841 cccacccgcg aggccgcgcc cgagggcagc ggggcgaagg tcgccgccct ccccgccggc
      901 cgccgctccc ctgcgtccaa agcctatggc gaggtgaagt ccgccgaagc ccggctcgcc
      961 gccgccctcg ccgccgacgc ccgcgccgcc ttgccccgct tcctcgccgc cgacggccgg
     1021 ctgatgcggg tcggccctca gcccgcggtc ggcgccgccg cctgggcgcc gcttctcgcc
     1081 gccggcccgg aacggatcga caccgccccc ctcggcggct ccgcctcggc cgcgggcgat
     1141 ctcgccttca cctacgggac cgcgcgatgg cgcaagggcg agaccggggt cgagggccat
     1201 tacgtccgca tctggcagcg ccgccgcgaa ggctggaagc tgatcgtcga caatatgatc
     1261 gcgatcccgc ctccgccccc ggctcggccg tccgcgggcg gatagttctg cgaaaccctc
     1321 cgctcatgcg acgaaagggg cggcgccctg cccacttcca ctataggatg ggacgcctta
     1381 cgccctggaa acgatcttcg atggccaaga ttatgccccg tccgaatgtc gcgttccagg
     1441 cggccaagct gactctcggc gtctggatct tcaccttcat cctcttcctg atgccggtcg
     1501 ccgtctcgat gggcgagctg ccgcgcttcg ccgtcgggat gaccgccctc gtcgtcgcca
     1561 tcggcatggc cctgaccgtc ccgctctact gggtctgcgc ccgcctccag gactcaccga
     1621 ccgcgctcaa gatcggcggg gtgttcggcg ccgtctgcgt caccgccttc gtcttctcgc
     1681 tgagcgacgc cttgttcgga gccaacatca tccagatgtt catgggcgag caccgcatcc
     1741 cgccggacgt gatcaaccgc accgtctcca acttcatcag cttctcctgg ctctacggct
     1801 ggcttgggac gacctacgtc atcctcctga ccaacgccgc gatgcgcgag cgcgacctcc
     1861 agctcgccga ggcgcgcacc ctcgcccaga ccgcccagct caccgcgctc cggctccagc
     1921 tcaatcccca tttcctgttc aacaccctga acgccatctc ttccctgatc gtcaccggcc
     1981 gcaatcgcga cggcgaggcg atgcttgcga aactgtgcgc cttccttcgc accgctctgg
     2041 tcggcgacgg gcgaagcgag gtgactctgg gagaggagct ggaaacgctc cagacctatc
     2101 tcgagatcga gtcgatccgc ttcggcgatc gcctcaccgt cgaattcgcc gcccccgaag
     2161 ccctgctcga atatccggtg cccaatttca tcctccagcc cctggtcgaa aatgcggtga
     2221 agcacggagt cgccccgacc agccgccccg tcatcgtcag ggtcggcgcc cgccgcgacg
     2281 gcgacgacct catcctgtcg gtcagcgaca acggaggcgc ggtcgcgggc tcgcgggtcg
     2341 cgggcaccgg agtcggcctc gacaacaccc gccgccggct ggaggtcatc tatggcccgc
     2401 gcggccgggt cgagacgctt gcccatgacg acggcttcac cgtgctcctg cgcgtcccgg
     2461 tgaatcaggc tccggcgctg gacctcgtcg catgaccctt cgcaccctac tcgtcgacga
     2521 cgagcccctg gccctggagc ggctccaggt cttcttccgc gacatcgccg gggccgaggt
     2581 gatcggcacc gcccgcgacg gccgggaagc ggccgaggcg atcgagcgcc tgaagcccga
     2641 cctcgccctg ctcgacatcc agatgcccga gcagaacggc ctcagggtcg cagccaccct
     2701 tccgccggaa gaccgcccgg agatcatctt cgtcaccgcc ttcgagcaat atgcccccga
     2761 cgccttcgag gtcgaggcgg cagactatct tctgaaaccg gtccgcttcg accggctctg
     2821 gcaggcggtc gagcgcgcca agcgccgcaa gcaccagcgc accctcgccg gccgcgccga
     2881 agccgcggcc tcgccccggg accatggccg cgaggacggc ctctgggtcc cgacccgcgc
     2941 cggcgcggtc ttcgtgcccg tggaggcgat cgagtggatc gaggcccagc gcgactatgt
     3001 catgctccac accgcgaccc gaagccacat cctgcgcatc tcgatgcgcg cgctcgagga
     3061 caggctcgat cccgcccagc tcatgcgggt ccaccgctcg gccttcgccc gtcccgcgaa
     3121 tgtcgtagag gtccagcgcc tgggaagaac gatcaacgcc ctcgtcctac gcgacggagc
     3181 gatcgtccag gtcggcccga attattccag ggccgtggcg gagcggctca aggtggcgga
     3241 gtaggaagct ccccggcgaa ggccggggcc cagcggcgca gcgcggggcc tgaaactgga
     3301 tcccggcctt cgccgggaaa catactgcat cggcgagccc ccttccggac aatccccagc
     3361 tttatttcat ttcggcgacg gccgccggga acggccggtc ctgatctggg ttaacggcat
     3421 agggccgctt ccgccggccc ggaacagggg atgtcgcgcg tgccggggcc catgatattg
     3481 ctcgtcgagg atgagccgct cgtccttcgc aacaccgccg atctcctcat cgaggacggc
     3541 tacgacgtcg ccgaatgcac cggctgcgag gaagctttgg caaggctcga ggccgagccc
     3601 gacaccgccg tcctcgtcac cgatctcaac gtcgcctgcc ccggcgacgg gatgaccctc
     3661 gccaaggtcg ccgccgagcg ctggccgcac ctgcgcatcg tcatggtctc tggagcgctc
     3721 cgtccgcccc agggcagcta tcccgagcgc gccatcttct tcaccaagcc ctacgcgccc
     3781 ggcgccctcc tccacgtcgt caagtctccc gacttcgcct gaggggcggc acgccgtcat
     3841 tgcgaggagc gcagcgacgc ggcaatcccg gttctgcgtc ggaggccgca ctggattgct
     3901 tcgctacgct cgcaatgacg gagaagacct tcccacgccg cacggccgcg cctaacgctc
     3961 cggccatgga cgacgacctg ccggaggcca tcccggccgc caccctcatc ctgatgcggc
     4021 ccgcggccgc cggcggtccg cccgagatcc tcatgctcga gcgggccgag accatggcct
     4081 tcgcggccgg ggccctggtg ttcccgggcg gccggatcga tccgggggac gaagcggcgg
     4141 cgaaccgctt cccccggctc cccgacgccg ccgcccggat cgccgcgatc cgcgagacga
     4201 tcgaggagac cggggtcgcc ccggcgcttc gccccgctcc ggacgccatc gcgcttcgcg
     4261 aggcgatggc ggccggccgc ccgttcgccg ccctgctcga cgagatgaac ctgtcgctcg
     4321 atctcgaagc gctcaccccc ttcgcgcgct ggtgccccaa tttccgcgag gcccggcgct
     4381 tcgacaccct cttctatctg gccgaagctc cggcgggcag gggaacccgg cacagcgccg
     4441 acgagacgga gagcgtccat actttctggg ccagcgccgc gacggtgctc gccgagatcg
     4501 aggcgggccg cgccaaggcg atcttcccga cccgccgcaa cctcgagcgc ctcgcccgct
     4561 tcggctcgct cggcgaagcg cgcgcggacg cggagcgcca cccggtgcgg cagatcacgc
     4621 cctgggtcga agagcgcggc ggcaagcgct tcgtctgcat ccccgagggg atcggctatc
     4681 cggtgacggc ggagccctat gaaagcgccc ggcggcgctg acttcgcttg ggacggcgcg
     4741 aaaggcgcgc gaccgcgagg cggtccctcc ggagccggaa ggcggccagc cagaacatat
     4801 agctgagcat gagggcgata aggatcggga acgcgtacat catggcaaga taaagctccg
     4861 agactcagca agttccggca gtcgcggcga tatttcgtcg ccccggcgca ggcgacgaaa
     4921 tgacggctcc agtcgcaccg gcggccggta gcggcggcgt tcctcgatcg gctcgaccgg
     4981 ctgcgaccgg cgcgtgtcga cgaacagggt ctcgatgacg ctcggcccgg gataggcgac
     5041 cagcacctcc gctcccggat cgagccggac gcggctctgg tcctcgacca gccagaccga
     5101 ggccggctcg agcggctggc cgtcgatcag cccgcttccc ttgaccggca ccagccagac
     5161 cggccgctcg acctcgccgg ccagcccccc gcccgcgttc cgggtccagc gctcgacgac
     5221 gaacgccccg ccttccgcca gtatctcccg cccgggctcg atctcccggc gcggagtcgg
     5281 cgacacgtag ggttgcggcc gcgccgcttt caccccggcc tcgacctgaa gctcgcgctc
     5341 gcggccgtaa tcgaacaggc ggtaggtgag atcgacattc tcctggacct cgatcagggt
     5401 cagccccggc ccgagggcgt ggatcgtgcc cgccggcgaa taatagaaat ctccggcctt
     5461 cgcttcgcgc cagtcgacca gcgcctccag ggtccccgcc tcgatggcgg cgcgaagccc
     5521 ggccggcgag acctcctgcc tgaggccgag gccgatgcgg gcgccgggct cggcctccag
     5581 caccagccag gcctcgtcct tgccgcggcg atggcccgcg gcccgcgccg cttcgccgtc
     5641 gggatggacc tggatcgaca gcttttcccg ggtcagaaga tatttgacga gcagctccgc
     5701 ctccggaccg cgcccgtcct cgaaccagat ctcgccgatc ggctcgcccc cggatgccgc
     5761 ggccgcgccg aagctgcgcg gaagctcggc gcgtccccac aatttctcga cctgccttga
     5821 agcgaggcgg ctcgccgtca caccgggtcc tctcgagtta cgaaaccggc gccggtcgtc
     5881 cggagtcttg agggcatgag atacctgcgc ggggtaaaaa tcttcctagt gctggcgctg
     5941 ctcgccttcc tggcctttct cggctggggc tttgcgcgca accaccccga ggacgtgccc
     6001 tggaccgagc tcgatctcac ccggccggtc ggcgtcttca ccggccgcaa gctggcggcc
     6061 ctgagcgggg aaggggagaa atgccgcgcc ctcctgcgtc gcgccggagt ccgcttcgtc
     6121 gccctgccgc cgcgcggccg cgcgcaatgc ggctatgacg atgccgtccg cttctcctcg
     6181 ggcggtgcgc tcacgatcgg ctaccgcccg gcggacctcg gcaccagctg cccggtcgcc
     6241 gccggactcg cgctttggga gtggcacgtc gtccagcccg cggcgctccg ccatttcggc
     6301 accaaggtcg ccgcgatcga gcatttcgga agctacagct gccgccggat ctacggccgc
     6361 ccggaagggg cgtggagcga gcatgcctcg gccaacgccg tcgacatcgc cgctttccgg
     6421 ctctccgacg gccgacggat caggatcgtc gccgactggg agggcggcgg acccaaggcg
     6481 cgcttcctgc gcgaagtccg cgacggcgcc tgccggctgt tcgccaccac cctgtcgccc
     6541 gactacaacg cggcccatcg cgaccatttc cacctcgatc aggcggagcg cggcgcgatg
     6601 ggctggcgcg gctgccgcta gggtcccgac cccgcggcct agcgacagtt agcgagccgg
     6661 gcttctaaat tcctcccctg cattcgcagg ggagggggac catgcgaagc atggtgaagg
     6721 ggcctcgctg agttcgaaga acccctccac cgcgcttcgc gcggtccccc tcccccggaa
     6781 atccgggggg gaggaattaa gagggatcga cctggtctga atgtcgatag agcctacgcg
     6841 atgggcggcc ggtcggggac cggaaagacg taggtgccgc cgtccgcgag gcgcatcacg
     6901 ggcccgggat agcccggata gacggtcgcc cgcacccggc gctcgatctc gtccttcggc
     6961 atgtcgtgct cgatcacctt gagcgcttcc atctcgcggc gggtgaacgg ccgccgggtc
     7021 cagtgcacgc cggcccgcgg caggggccgc ccggcggcga tcagggcgac gatctcccgg
     7081 aacatgtcga ccatcgtctc catcgtccga agcttcaggg tctcgacgct gtcgtccggg
     7141 gtgactggaa agcgccgctc catgatgaga tcaccggtgt ccaccttcgc gagcatgtgg
     7201 tgacacacgg cgccgaactc cgcggcgccc tcgtaaagcg cgaaattgta gcagccgatg
     7261 ccgggatagt cggtcgatcc gggatggaag ttgatcgcgg cgccggccct gtcgaggctg
     7321 tgcttgcgaa cgatccaggg agacaggaac gagatgagat attcggggct gcccgtctgc
     7381 agcgcttcgg gctcggggtc gccgaccgcg cccgtatagg tggcgaggtc ggcgccgaag
     7441 gcggcctcgg cgatcgcctg ggcctcgcgg ctgaaccgct cccgcttggc gaggaggatg
     7501 atccgggatg acataggccg ccctttagcg cactggcctc ccgcggcaag gcggctgggc
     7561 gtcgccgaaa cgaaatcggc cccgcccgtc gccgggcgag gccgatatct tctccgccga
     7621 agcggaaagc ttcaggtcag atggcgcgag agatgcttgt tcatctcgaa catcgagcag
     7681 cggtcggttc cgaagatctt cttgagcttg tcgtcggcga cgatctcgcg cttgttttcc
     7741 gggttctgca gcttgttggt gcggatatag tcccacatct tgctgaccac ctggctgcgc
     7801 ggcagcgggt ccgagcccgt gatcgccgcc agatccgccg acggcgtcac cgggcgcgac
     7861 agtccgcccc tcgctccgcc gcctgctgaa gtgccctttg ccatagtttt ccctcctgtt
     7921 tatgctccgc tgataagccc caaacgcgcg gcgagcgcaa gagattccaa gaggggaatt
     7981 cgtcggatcg cccctgaggg ccgttccgac gcaaatttaa tgtggatcat tccccgattc
     8041 ggcccctagc gccggccgaa cggcaccacc ttgttgccgg cgtcgtggcc gatatcggcg
     8101 cggcccagat aggggatccc ggaacggccg caccaatgaa ggaacacctc ctcctcgctt
     8161 gcgccgaagt ccggatcgtt gtccgggacc tcggtgcagc ggcccagcct gatcccggcc
     8221 acccggcgga ctcccgcatt gccggtgatg tggaagagcg atcgatcggt ccggtacata
     8281 tgctcggaga catcctcgag catcagcaca tgaccggcca ggtccggttc gagcgcagtc
     8341 ccgagcagct gggagaagac ggtgatgttg aacgcggccg ccggtccgtc gcccagactg
     8401 ggctccaggg cggccggatc gccccggacc agccaggaca gcgcccgcgc aaccgcggtc
     8461 tcaccgccgt cgcggcgcac gtccccgggc atcgggccat gcgcgaggcg ctcgaagccg
     8521 gcgcggtaga gcgccgccag cacgtagccg gcatcgctat agcccagata ggtcttgccc
     8581 cgggccgcag gccccagccg cgcgaccgca tcctcggcga tccggcacga tccgtaaccg
     8641 ccgcgggcga cccagagggc gtcgaagccg ggatcgttcg cgacctcgac gaaagcctcg
     8701 gcccgcgccc cgtcgtcgcc ggcgaagtgg cggtggctga gaaagcattg cggatggaag
     8761 accagctccg ccccggggac ggcctgcgcg gcgatcgcct tcacccgctc cgccgtctcg
     8821 ggcaggatcg gagtgctcgg cgccacgatt ccaatgcgca ttccctcacc ctcagcgttt
     8881 gaaaagccac gtcgaccgca atagggcggc gccatgtcag gaaccaaatc ctatttcttc
     8941 tgcggcatcg gcggcagcgg catgctgccc ctggccgcga tcgtccgcgg ccagggcgcc
     9001 cgggtctccg gctccgaccg ggcgctcgac cagggccgca ccgccgccaa gttcgattat
     9061 ctgcgcggcc tcggcataga gctctacccc caggacggaa gcggcccccg ggcgggccag
     9121 atcctggtcg cctcggccgc ggtcgaggag agcgttcccg acgtccaggc cgcccgccgc
     9181 ctcggcctcg agcggatcac ccgcgccgag ttgctctccc gactgttcaa cgccgccgcg
     9241 accgggatcg ccgtcggcgg caccagcggc aaatcgaccg tcaccggaat gatcgcctgg
     9301 atactccacc gggccggcct cgagccgacc gtgatgaacg gcgcggtgat gaagaacttc
     9361 gcgacgtcag acgctccctt cgcaagcgcc ctggtcggcg gcgaccgggt cttcgtcagc
     9421 gaggtcgacg aaagcgacgg atcgatcgcg ctctaccggc cgaagatcgc cctgctcaac
     9481 aacgtcagcc tcgaccacaa gtcgatggag gagctgcgcg ccctgttcgg cgatttcctc
     9541 ggccgggccg ggacggcggt ggtcaatctc gacgatccgg aggctcgggc cctggcgggc
     9601 gcgggcgccg tcaccttcag cctcgccgac ccgcgggcgg actttaacgc ctccgggatc
     9661 gtcgaggcgc cgaccgccgt ccagttcagg gtcgcggaaa ggtccagcgg cgaggccgcc
     9721 gcggttcggc tcccggtccc cggccggcac aacgtcgcca acgccctcgc cgcactcgct
     9781 tcggcccgag ccgccggggt cgcgctcgcc gccgccgcgg aggcgctgtc gagcttcgcc
     9841 ggcctgcgcc gccgcttcga tatcgtcggc accaggggcg gggtcacggt catcgacgat
     9901 ttcggccaca acccggacaa gatcgccgcg actctggcca cctgccacgc ctttccgggc
     9961 cggctgctcc tgttcttcca gccccacggc tacgggccgc ttcgaaccat gcgcaaggag
    10021 ctcgtcgccg ccttcgccgg caacatggct cgggaggacg ttctgatcct ttgcgacccg
    10081 gcctatttcg gcggcaccgt cgagcgcacg gtgaccagcg ccgacctcgt cgccgacgtg
    10141 gccggggccg gccgaagcgc ggagcacatc cccgatcggg cggaggccgg ccgccggctc
    10201 gcggccctcg cccgccccgg cgaccgaatc ctcgtcatgg gcgcccgcga cgacacgctc
    10261 agccagttcg cgggcgagat actgaattgc ctccccatgc tgcgcatggg gaggcaaatc
    10321 taggacagcg cctcgattac gaaagcaatg aggctcgccg ccagccccgc caggaagatg
    10381 cgataggccc agcccagcat ccggtacttc ttccgggcga gcacgattcc gttctggtag
    10441 atgtcgcgcg ccatcgtccg gtagatgctg tcgtcgctgc gcagctcggt cagcagccgc
    10501 tcgatataat cgtcctcgtc gagccgggtg aacgatccga agaacatcag gttgagcggc
    10561 gcgcgcttgc gatagccgac ggccgggagg atcgcgagca ccgcgaacac cgccgacagg
    10621 aaggccgctc cgcccaggat aagcagcggc agcggcgcgc ctttcccgtc cgaatggccg
    10681 accgcgatgg tgaagatgac gaaggcggcg cccatcagga tgctcgcttt ctggtcggcc
    10741 atggcggaaa ggttcatctg cgcggaggcc gcggtacggg ccagatgcac cgcgctcggc
    10801 ggataggccg gcggcccgcc tttctcctcg tccgccatcg ccgcacccct tgtccttcgc
    10861 gccgcgctcc atggcatggc cctcccgccc tgccaagctc cggccccaat gcggccctaa
    10921 tcgcttggca gagcaacgcg ccggacataa ggggctggga tgaccgacag agaagagact
    10981 ttcgccaagg tcgtgcagct catcgagccg ttcaacaaga aggacgccga gctcagcgat
    11041 gcgaccacct tcgccggcga tctcgaatgg gacagcctca tcgtcatgga tttcgtcgcc
    11101 tccgtcgagg acgagttcga tatcttgatc acgatgaaca tgcaggcgga gatcgagacg
    11161 gtcggccagc tcgtcgacgc cgtcgaagag ctccggaacg acggatgacc gaagccgccc
    11221 gcaacgccat ggatcccgcc ggcgagccgg ccggccatac ccacggccgc gatctcttct
    11281 ccaagttcga cgctctgatc gccgagcgcg acaccttgct cgacaccggc gtgcgcgacc
    11341 cgttcgggct ggtcatggaa gaggtgctgt cgccgacccg ggcgatcgtc aagggcaagg
    11401 agacgatcct tctcggtacc tacaattaca tgggcatgac cttcgatccg gacgtgatcg
    11461 aggccggcaa gaacgccctc gacgccttcg ggtcgggcac caccggcagc cgggtcctca
    11521 acggcaccta ccagggccac atggaggtcg aggaggcgct gaaggacttt tacggcactc
    11581 gcgacgccat ggtcttctcg accggctacc aggccaatct cggcctgatg tcgacccttg
    11641 ccggcaagga ggattacatc atcctcgacg ccgacagcca cgcctccatc tacgacggct
    11701 gcaaattggg cgacgcccag atcgtccgct tccgccacaa caacgtcgag gatctcgaca
    11761 agcgcctggg acggctcccg gcccaggggg gaaggctggt ggtgctggag ggcgtctatt
    11821 cgatgctcgg agacatcgcg ccgctcaagg agatggtcga ggtcgccaag cgccacggag
    11881 cgatggtggt ggtcgacgaa gcccacggga tgggcttctt cggccccaac ggccgcggtg
    11941 ttttcgagga agccggggtc gaggaccagg tcgatttcgt cgtcggcacc ttctccaagt
    12001 cggtcggcac cgtcggcggc ttcgtcgtct ccaaccatcc caagttcgga gtgctccgac
    12061 tcgtctgccg cccctatgtc ttcaccgcct ccctgccgcc gagcgtcgtc gccaccgccg
    12121 cgacctcgat ccgcaagctg aagttcgccg gcaacaagcg ccagcacctc tgggagaaca
    12181 gccgccgcct ccacaagggc ctgcgcgacc tcggcttcga cctcgccacg cccgaggcgc
    12241 aatcggcgat catcgccatc ctcctgcccg accaggacag gacggtgcgc atgtggcagg
    12301 cgctcctgga agcgggagtc tacgtcaaca tggcccgccc gcccgccacg cccgccggaa
    12361 tgtacctgct gcgctgctcg ctctgcgccg accatagcga agagcaggtg acccagatcc
    12421 tcgccatgtt cgagagcgcc gggaaagcaa ccggaatcct gcaataacag caacttagtc
    12481 aaagtgactg tcactagtga cagtcactag tgacagtcac ctagtcaaag tgacagtcac
    12541 tagtgacagt cacccgcccc aaagtgactg tcactagtga cagtcacccg cccatggccc
    12601 acctagccca atgcccacca cagcatcccc gccgcagcgc agcccgccag caccaccggc
    12661 gcccccagcc tgaaccggaa catcgccacc aaagcggcgg cggcgagggc gcaggcggcg
    12721 aggtcgacgc tggccggcac cggcaggtcg aagcggagcg gccccgccgc cacctcgcgc
    12781 accacgcgga acaaggtgtg gagggcgaac cagatcgcga ggttcaggac cactccgacc
    12841 acggccgcgg tgatcgccgt cagcgccgcg gtgagcgcgc ggttctcgcg cagcttctcc
    12901 acgaacggcg ctccgaggaa tatccacagg aagcaggggg tgaaggtcac ccaggtggtg
    12961 agcagtccgc ccagcgtccc cgccaccatc ggatgaagcc cgcccgggtc gcgataggcg
    13021 cccatgaagc cgacgaactg ggtgaccatg atcaacggac cgggcgtggt ctccgccatg
    13081 cccagcccgt cgagcatctc gccgggcgcg agccagccgt aagtctccac cgcctgctgg
    13141 gcgacataag cgagcaccgc ataggcgccg ccgaacgtca ccaccgccat ttgcgagaag
    13201 aacaggccga tcttgctgaa cacgctgtcc gggccggccg tcgcgagcaa cagggccaaa
    13261 ggtcccagcc agaggagaag gaagatggcc gagatccgaa gcgcccagcg caggctcggg
    13321 acggcgtgcg cgggtatccg gtcgccgagc agggacttgc cggcgggctc ggggccatgg
    13381 ccgccgccgc cgctgaaggc gcccgccccg gagcggccgc cggcatagcc ggccaggccg
    13441 gcggcgagca cgatcagcgg aaacggcact gcgaagaaga agatcgcgac gaacgccgcc
    13501 gcggcgatac cgcgcaggac attgttcccg agcgcccggg cgccgatccg ccgcaccgcc
    13561 tcggcgacga tcgcgagcac ggccgccttg aggccgaaga acagggccgc gacccagccg
    13621 acattgcccg ccgcggcgta gatccaggac aggatcatga tcgcggcgaa gccgggaagc
    13681 acgaacagga ttccggcgat cagcccgccc ttcgtccggt gcatgagcca gccgatatag
    13741 gtggcgagct gctgcgcctc cgggccgggc aggagcatgc agtaattgag cgcgtgcagg
    13801 aacctggcct cgccgatcca gcggcgttcc tccacgagca cccggtgcat cagggcgatc
    13861 tggccggcag gcccgccgaa gctgagcaag gcgatcttcg cccagacgcg caccgcctcg
    13921 gcgagcggga ccgaagtctc cggctcgggc ggccgctcgg ttgaagcgga ggacgacatc
    13981 gggccctgac gtctcatcgc gccttgcgtt ccttcggcgg acaagctccc gctcccccgg
    14041 ccgggagggg gccgcaggca cgacagccgc cccccaaagc gcggcgaacc gatggtcggg
    14101 ccacggaact ttctttccat cccctccctg cttgatctag actcgacgcc atggcgggcg
    14161 aggacatgta cgccatgtcc gaggaaagga cttccggggc atggcgcgga gtgctggcga
    14221 gcgcgggtgc cgtgctcgcg gccctgctgc tcgtcgcctt gatgctgatg gtcacctcct
    14281 cgaacgaggt gcgggaccgg gctctggcgt cggagcgcca cagctacgac gtcatgctcc
    14341 tcacccgaac ggtcgacggc accatagccc gggccgaggc cgcgctcgga cgctacgtcc
    14401 tcgacgagga cgtcgcgacg agcggaagcg tctacggcgc ggaatggcgc caggcgcagc
    14461 agctcatcgc cgagctcagg cggcaggtcc gcggcaacaa ggagcagctc gcccgggtcg
    14521 aggcgctgga gaaactgttc gcgacccgcg gccgcgagct ggcgccggcg gcggtggccg
    14581 cccaggccaa ggccggcagc ggcggcatct cgctattcta tggcgcggcg cggactccga
    14641 ccgggcagca gctgcgcaac actctcgccc agatcgccac cgccgagcgg gaggatctgc
    14701 gccggcgaat ggcggccacc cgagtgtcgg gcacccgctc cgaccagctc accgaatggc
    14761 tcggctggct cgcggtcgcg atcgggcttg gagcgatcgt gctcggctgg ctcgccttcc
    14821 gggcgatcag cggccggctc gcggcgctcc gcgaggccga cagcgaggcc aaccgcgccc
    14881 gccagctcga ggaagcggtg cgcgaacgta cgcgcgagct gagccaggcc tatgagcagc
    14941 tcaaggcgga atcggccgag cgcgaggcgg ccgaggcgca gcttcgccag gtccagaaga
    15001 tggaggccgt cggccagctg acgggcggca tcgcccacga tttcaacaac atgctcgcgg
    15061 tcgtcgtcgg cggcctcgat ctggcgcggc gtaagctcac cggcccccgc cgcgaggtcg
    15121 agttccacct cgacaacgcg atggagggcg cgacccgcgc cgccgcgctc acccgccggc
    15181 tgctcgcctt cgcccgggcc gagcccctgc tcccggtcgc cgtggctccg gccggcctcg
    15241 tcgaaggcat gctcgacctc atcgaccgct cgatcggcga gcggatcacc gttcgcaccc
    15301 gcttccccga ccggacgtgg aacgtcagcg tcgacaccaa ccagctcgag aacgccatcc
    15361 tcaacctggc cgtcaacggt cgcgacgcga tggacggtga aggccagctc gccatcgccg
    15421 tcgagaatgt cgtcctctcc gccggcgaag cgggtgagct cggcgcgggc gactatgtcc
    15481 ggatcagcgt ctccgacacc ggctcgggca tgccgccgga agtattggaa cgggtgttcg
    15541 agcccttctt caccaccaag ccgaccggca agggcaccgg gctcggcctc tcacagatcc
    15601 acggcttcgc ggcacaatcg ggcggcgagg cgcggatgga gtcggaggtg gggcgcggaa
    15661 ccacgcttca cttcctgctc cccaggacga agaaggaacc gcccagccag gccgcgcccc
    15721 agctcgacat cgcgctgccg cttgggctca ggatattgct ggtcgaggat agcgagcatg
    15781 tccgctattt cgcgcgccat cttctcgagg atctcggctg cgcggtggtt gaagccgccg
    15841 acgcggaaca ggcgctggcg ctgctc
//
LOCUS       DASWNU010000654         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_102851, whole genome shotgun sequence.
ACCESSION   DASWNU010000654 DASWNU010000000
VERSION     DASWNU010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102851"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VGB57_13185"
     CDS             complement(<1..168)
                     /locus_tag="VGB57_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0132347.1"
                     /translation="MTDEGARPAVLVTGGAGYIGSHAVLALLDAGWRVVVIDNLTTGF
                     RWAVPGAATFVE"
     gene            258..833
                     /locus_tag="VGB57_13190"
     CDS             258..833
                     /locus_tag="VGB57_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY0132348.1"
                     /translation="MKAVFLRAGALLLAASLAGCATNQGGPGAHSGTEVTRFHLGNPI
                     ARGQIAIEAYEPRDSATPEFRVFADIVARELTRLGWTVVPTTTQSEQVALIDLEQGSR
                     EAVAALSAARIGRGVAGTAPAGASANVTASLLEVAIRRRSDGTVFWEGRAVGEAPTGS
                     AEATRTAAVERLAAALFRDFPGESGRTIRVR"
     gene            830..1963
                     /locus_tag="VGB57_13195"
     CDS             830..1963
                     /locus_tag="VGB57_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEY0132349.1"
                     /translation="MTLSVTSPFDGGNIRTVAIEGNRIDLEIVKDHSSDFYQWFYFRL
                     TGGAGQEVELRIVNCAGAAYPLGWQNYRACMSVDREEWERVDTRYEGGILSIRLTPPA
                     NSVWFAYFAPYTMERHHDLVASVAGHPAVEHRSLGRTFDGQEMDYLRIGEGPVQVWLY
                     ARQHPGETMAEWWMEGALERLLDEADPVARRLLAKATFHIVPNMNPDGSRRGHLRTNA
                     VGTNLNREWHDPSPERSPEVLCVRNEMDRTGVDFAMDVHGDEAIPANFLAGFEGIPSW
                     SEGRQKLFDRFSEELVRFSPDFQTRKGYEVARPGEANMSMSTSQLAERFGAVSMTLEM
                     PFKDNDDLPDPRRGWSPERSAHLARACLDALHAIIDDLPARKR"
     gene            1963..>2644
                     /gene="gpmA"
                     /locus_tag="VGB57_13200"
     CDS             1963..>2644
                     /gene="gpmA"
                     /locus_tag="VGB57_13200"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY0132350.1"
                     /translation="MPRLVLIRHGQSAWNLENRFTGWWDVDVTEKGAEEARLAGELMA
                     ARGIDFDLCFTSLQTRAIKTLNLALEAMGRLWLPVEKDWHLNERHYGGLTGLNKAETA
                     AKHGDEQVRIWRRSFDIPPPPMEEGSPYDLAADRRYAGIDVPRSESLKDTIARVLPYW
                     DGRIAPALRDGQRVLISAHGNSLRALVKHLSNIPDDEITSLEIPTGQPIVYELDDGLN
                     EVERYYLKD"
BASE COUNT          455 a          921 c          858 g          410 t
ORIGIN      
        1 ctcgacgaag gtcgctgcac ccggcaccgc ccagcgaaag ccggtggtga gattgtcgat
       61 cacgacgacc cgccagcccg cgtcgaggag ggcgagcacc gcatggctgc cgatatagcc
      121 cgcgccgcca gtcaccagca ccgccggcct tgcaccctcg tccgtcatgc aaattccccg
      181 tattcgcctt tgcagcgaaa cgctcctagg atcgttccac cgccgtataa ttggccgctt
      241 agcaacgagg ttccggaatg aaagctgttt ttctccgcgc gggcgcgctc ctcctggcgg
      301 catcgctggc gggctgcgcg accaaccagg gggggcccgg tgcccactcg ggcacggagg
      361 tgaccaggtt ccatctcggc aacccgatcg cacgcgggca gatcgccatc gaagcctatg
      421 agccacgcga cagcgccacg cctgaattcc gcgtcttcgc tgacatcgtc gctcgcgagc
      481 tgacccggct cggctggacc gtggttccga cgacgacgca atccgagcag gtcgccctca
      541 tcgacctcga gcagggcagc cgcgaggcgg tggcggcgct gagcgcggcc cggatcggcc
      601 gcggagtcgc cgggaccgct cccgccggag catccgccaa cgtcaccgcg agccttctcg
      661 aggtcgcgat ccgccgccgc tccgacggca ccgtcttctg ggaaggccgg gccgtcggcg
      721 aggcccccac cggaagcgcc gaagcgaccc ggacggccgc cgtcgaacgg cttgccgcgg
      781 ccctgtttcg ggacttcccc ggggagtcgg gccgcactat cagggttcga tgaccctatc
      841 cgttaccagc cccttcgatg gcggcaacat tcgcacggtc gccatcgagg gcaaccggat
      901 cgacctcgaa atcgtcaagg accacagctc cgatttctac caatggttct atttccggct
      961 gaccggcggg gccggccagg aggtcgagct caggatcgtc aactgcgccg gagccgccta
     1021 tccgctcggc tggcagaatt accgggcctg catgtcggtc gatcgggagg aatgggagcg
     1081 ggtcgacact cgctatgaag gcggaatcct ctccattcgc ctgactccgc cggccaacag
     1141 cgtctggttc gcctatttcg cgccttatac gatggagcgg caccacgatc tcgtcgcctc
     1201 ggtcgccggc cacccggcgg tggagcaccg ctcgctcggc cggacgttcg acggccagga
     1261 gatggactat ctccggatcg gcgagggccc cgtccaggtc tggctctacg cgcgccagca
     1321 tcccggcgag acgatggcgg aatggtggat ggagggcgcg ctcgagcggc tcctcgacga
     1381 ggccgatccg gtcgcccgcc ggctgctcgc caaggcgacc ttccacatcg tcccgaacat
     1441 gaatcccgac ggctcgcgcc gcggccattt gcgcaccaac gcggtcggaa ccaacctcaa
     1501 ccgcgaatgg cacgatccga gccccgagcg cagcccggaa gtgctgtgcg tcaggaacga
     1561 gatggaccga accggggtcg atttcgcgat ggacgtccat ggcgacgagg cgatcccggc
     1621 caatttcctc gccggcttcg agggcattcc aagctggagc gagggccggc agaagctgtt
     1681 cgaccgcttc agcgaggagc tggtccgctt ctcccccgat ttccagaccc gcaagggcta
     1741 tgaagtggcc cggcccggcg aggcgaacat gtcgatgtcc acctcgcagc tcgccgaacg
     1801 cttcggagcc gtctcgatga ctctggagat gccgttcaag gacaatgacg acctgcccga
     1861 cccgcgccgc ggctggtcgc ccgagcgaag cgcccacctc gcccgagcct gcctcgatgc
     1921 gctccacgcc atcatcgacg atctgcccgc ccggaaacgc tgatgcccag gctcgtcctc
     1981 atccgccacg gccagtcggc gtggaacctc gagaatcgct tcaccggctg gtgggacgtc
     2041 gacgtcaccg agaagggagc cgaggaggct cggctcgccg gggagctgat ggcggccagg
     2101 gggatcgact tcgacctgtg cttcaccagc cttcagaccc gggcgatcaa gaccctcaac
     2161 ctcgccctgg aagcgatggg gcggctgtgg cttccggtcg agaaggactg gcatctgaac
     2221 gagcgccatt acggcgggct caccgggctc aacaaggccg agacggcggc gaagcatggc
     2281 gacgagcagg tgcggatctg gcgccgctcg ttcgatatcc cgccgccgcc gatggaggag
     2341 ggcagcccct acgatctggc cgcggaccgt cgctatgccg gaatcgatgt gccgcgctcc
     2401 gagagcctca aggacacgat cgcccgggtg ctgccttatt gggacgggcg gatcgctccg
     2461 gctcttcgcg acgggcagag ggtgctgatc tccgcccacg gcaattcgct gcgggcgctg
     2521 gtcaagcatc tctcgaacat tccggacgac gagatcacga gcctcgagat cccgaccggg
     2581 cagccgatcg tatatgagct cgacgacggg ctgaatgaag tcgagcgcta ctacctcaag
     2641 gacc
//
LOCUS       DASWNU010000655         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_284799, whole genome shotgun sequence.
ACCESSION   DASWNU010000655 DASWNU010000000
VERSION     DASWNU010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284799"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGB57_13205"
     CDS             <1..492
                     /locus_tag="VGB57_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132351.1"
                     /translation="TLTATYRSHNLLWAWPENVYGLMAIQRHVAEGAGMETGPITVIS
                     NSLGINPESPRYELAKAIERGWKTDDDLDRDSGKYSLREDPNGYFVVTVDKEEGKLVA
                     EHRFGGVLVKRYEADRAVTIEREVAADMAISLVSHAMWLGRELTLKERALRGSAPGRD
                     EDG"
     gene            485..1207
                     /locus_tag="VGB57_13210"
     CDS             485..1207
                     /locus_tag="VGB57_13210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0132352.1"
                     /translation="MAEPGRSEDDGPVVFLDIGATLVGADVKGPASRIGALLGLDGEG
                     RRALRGALMTTDFWTPEEVAAHLVVSLGAEPEAAARASREIWESQRADARPLPGAPEA
                     LARLAAEGCRLGLISNIWRPYLESVRSHYGALFDDHVEPRLQLFSFQVGAAKPGPGIF
                     TEALRRAGVGPERAVMVGDSYDEDVAPAAALGMRTVWVLTRPEDQAEAIGHVEDGAAP
                     PPTFTVDSLDALDPRLLSCADG"
     gene            1227..1847
                     /locus_tag="VGB57_13215"
     CDS             1227..1847
                     /locus_tag="VGB57_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132353.1"
                     /translation="MLEVETFGREELLDALRETRLRGFDGARVYADATLELVESDPAE
                     LTPAQRYVLREGVEKILGLQAALLERGIDVFALEGGLWVRTADSPEERIPVIPPIVEE
                     SREPDGRTVWIVNDGLHRVFAARSLGCSINTVRVAGVPPEYPYYALALPGGWAEVAEL
                     DELPDGFQKKEYRQPDNYKALFRKFNDVFPGVQKERKNSNPGFLQA"
     gene            1856..>2092
                     /locus_tag="VGB57_13220"
     CDS             1856..>2092
                     /locus_tag="VGB57_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132354.1"
                     /translation="MTAGDRQPGLRPVDELISDLEAGAGSIAAVANKRCLDIDVLSGL
                     HLDLPRAETDGGGEAELARVLYRRYQKLREPFSED"
BASE COUNT          319 a          715 c          768 g          290 t
ORIGIN      
        1 acgctgaccg ccacctaccg ctcccacaac ctgctctggg cctggccgga gaacgtctac
       61 gggctgatgg cgatccagcg ccatgtcgca gagggagccg ggatggagac cggcccgatc
      121 accgtgatca gcaactcgct cgggatcaac cccgagagcc cccgctacga gctcgcgaag
      181 gcgatcgagc gtggctggaa gaccgacgac gacctcgacc gggacagcgg gaagtactcg
      241 ctgcgggagg acccgaacgg ctacttcgtc gtcaccgtcg acaaggagga gggcaagctc
      301 gtcgccgagc accgcttcgg gggcgtgttg gtcaagcgct acgaagccga ccgggcggtg
      361 acgatcgagc gcgaggtcgc cgccgacatg gcgatcagcc tcgtctcgca cgcgatgtgg
      421 ctgggccgcg agctgactct gaaagagcgg gcgctgcggg gctccgcccc tggtcgcgac
      481 gaggatggct gagccgggcc gaagcgagga cgacgggccg gttgtctttc tcgatatcgg
      541 tgcgacgctg gtcggcgccg acgtcaaggg gccggcctca cgaataggcg ccctgctcgg
      601 gctcgacggc gagggccggc gggcgctgcg cggggcgctg atgaccaccg acttctggac
      661 gccggaggag gtcgccgctc acctcgtcgt ctcgctgggc gccgagccgg aggcggcggc
      721 gcgggcctcg cgggagatct gggagtccca gagagccgac gcgcgtccgc tgccgggagc
      781 cccggaggcg ctcgcccgtc tcgctgccga aggctgcagg ctgggcctga tctcgaacat
      841 ctggcggcct tacctggaat cggtccgcag ccactacggg gccctcttcg acgaccacgt
      901 ggagccgcgc ctgcagctct tctccttcca ggtgggagcg gcgaagccgg gccccgggat
      961 cttcaccgag gcgctgcggc gggccggggt ggggccggag cgggcggtca tggtcggcga
     1021 ctcctacgac gaggacgtcg cgccggcggc ggcgctcggc atgcggacgg tctgggtgct
     1081 gacccggccc gaggatcagg cagaggcgat cgggcatgtc gaggacggcg cggcgccgcc
     1141 gccgacgttc acggtcgatt cgcttgacgc tctcgacccg cggctcctat cttgtgccga
     1201 tggctagggg gggcgaggcg attgaggtgc tcgaggtcga aaccttcggc cgcgaggagc
     1261 tgttggacgc cctccgcgaa acccggctgc gcggcttcga cggcgccagg gtctacgcgg
     1321 acgcgacgct cgagctggtc gagagcgacc ccgccgagct gacgcccgcc cagcgctacg
     1381 tgctcaggga gggggtggag aagatcctcg gcttgcaggc ggccctgctc gaacggggga
     1441 tcgacgtctt cgcactcgaa ggcgggctct gggtgcggac cgccgactcg cccgaggagc
     1501 gcattccggt gatcccaccg atcgtcgagg agtcccggga gccggacggc cgcaccgtct
     1561 ggatcgtcaa cgacggcctc caccgcgtct tcgccgcccg cagcctcggc tgctcgatca
     1621 acaccgtgcg ggtcgccggc gtgccgcccg agtaccccta ctacgccctc gccctccccg
     1681 gcggctgggc cgaggtcgcc gagctcgacg agctgcccga cggcttccag aagaaggagt
     1741 accgccagcc cgacaactac aaggccctct tccgcaaatt caacgacgtc ttcccggggg
     1801 tccagaagga gcgcaagaac tccaacccgg gattcctgca ggcatgaacg gcgcggtgac
     1861 cgctggcgat cgccagcccg gcctgcggcc ggtcgacgag ctcatctccg acctcgaagc
     1921 gggcgccggg tcgatcgcgg ccgtcgccaa caagcgctgc ctcgacatcg acgtgctgtc
     1981 ggggttgcac ctggacctgc cgcgggcgga gacggacggc gggggggagg ccgagttggc
     2041 tcgggtgctc taccggcgct accagaagct tcgcgagccc ttcagcgagg ac
//
LOCUS       DASWNU010000656         6180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_102867, whole genome shotgun sequence.
ACCESSION   DASWNU010000656 DASWNU010000000
VERSION     DASWNU010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102867"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGB57_13225"
     CDS             <1..186
                     /locus_tag="VGB57_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132355.1"
                     /translation="RTAGAEAIVHYVTDLSCASAGRCTARGGYLEGNLSASGNRYELE
                     RRNGRWQVTADTMEWIS"
     gene            282..1994
                     /locus_tag="VGB57_13230"
     CDS             282..1994
                     /locus_tag="VGB57_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132356.1"
                     /translation="MHLLRLLMSWSVRRVLVFVLLVAAMAAFVQVRSAYQRLPELRQQ
                     VESLERQQGLLEAEVDGQRKQAEARLQAIGSAEEPVLRRRLADVRAAIAGQEGGQSGG
                     LGLALDAARGDGNAVAGELAGRFGLQLLRREEAVIVARLDAIRSGGQVNGLVARIGAL
                     DSRIRALERSVAAIERRYPLLARIERVPGVRRIEGPWQELRAARQELAAARSERQRLA
                     PLYLTARGAFDQAGTAYKKSQAAVRELPAPTGELRRQIEQKRAQLAGHWASRAWAAVK
                     PVLGWALWVMLLILVTPPAVKAFWFFAVAPLASRLRPIRIGRPGRGELRWAAERLADG
                     EETRTGSAVSRRLLLRPGERLLVRPEYLQSSVVGAKADSQLLLSPALPLGSLATGLVG
                     LTRIRPEQETSAAISATRDLIDEIGLLEIPEGSAIVFRPRNLVGIVLTGSQPLRLERV
                     WRAACLMSWLTLRLRHLVFHGPCALVVKGARGVALEPAAGGRRVADAATMGWSAGLDY
                     SVTRSETFLAYLTGKQSLFHDRFEGESGQVVYEQMPRARAKSGLFGRGIEGLGDGLLK
                     IAGL"
     gene            complement(2002..2451)
                     /gene="rnhA"
                     /locus_tag="VGB57_13235"
     CDS             complement(2002..2451)
                     /gene="rnhA"
                     /locus_tag="VGB57_13235"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY0132357.1"
                     /translation="MTVPIVEIFTDGACKGNPGPGGWGAVIRSGPHEKEISGGEPLTT
                     NNRMELLAAIRALEALKRPCEVALYTDSIYVRDGITKWIHGWRRNGWKTADRKPVKNA
                     ELWQELLDSAAPHKVDWRWVKGHSGHPENERADKLACDAAMGFAKAR"
     gene            complement(2448..3395)
                     /gene="ispH"
                     /locus_tag="VGB57_13240"
                     /gene_synonym="lytB"
     CDS             complement(2448..3395)
                     /gene="ispH"
                     /locus_tag="VGB57_13240"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0132358.1"
                     /translation="MASKPPLTLLIAAPRGFCAGVDRAIRIVELALERYGAPVYVRHE
                     IVHNKFVVDSLKAKGAIFVPELDQVPDGVPVVFSAHGVPKAVPARAQERGLEYLDATC
                     PLVSKVHRQAERLVENDRHILFIGHAGHPEVIGTFGQVPEGRMTLIESVEDAQRVAPA
                     DPDGLAYLTQTTLSVDDTAEIVAILERRFPGIRGPKGEDICYATSNRQAAVKDIARAC
                     EAMLVIGSPKSSNSVRLVEVAEREGTRARLIGRASDIDLGWLAGVATLGITAGASAPE
                     TLVREVVDFLGRHYDVTELEGEGVIERMVFKLPRGLEAA"
     gene            3486..4127
                     /locus_tag="VGB57_13245"
     CDS             3486..4127
                     /locus_tag="VGB57_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132359.1"
                     /translation="MFKSKAALAALIPLGLAACSTPNGQITDTGITATRSACPAVAIP
                     AATGDITLFNPPSSRDAAAIDVVANITNLRSTCDESGANIVTTLTFDVHAQRRDGRGA
                     REVVLPYFVVAVQGGTQVQSKSVSRVGLSFADGALRASGRGSATGSVTRAAVTLPADI
                     TAQITRRRKPGDPSAAIDPMADPTVREAVRRATFEMLVGFQLTEEQLAYNATR"
     gene            complement(4274..5377)
                     /locus_tag="VGB57_13250"
     CDS             complement(4274..5377)
                     /locus_tag="VGB57_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132360.1"
                     /translation="MEHNSNISAQASIDERADPSPRAAGELSAHPADDAQFATQAEAD
                     AWWAGVSHGLRCATGENSRVDDEPVAPRDGRGEAAEVFDPSGRRLRVDGWLPWKKARF
                     LLVLVAGGVVADACRSVGMSVASAYALKNRRSGRAFGKMWDAILIHRARNRLADNNLS
                     RAMNGCVEQVLKDGVVIAERRRFDNRLSMAMLTRLDRLAESKDSEEAELLRTLSEDFE
                     DYLEVVEAGGDEEAFIEARRPREPEESGRPGFRRDSLDSLAGLSGCSDWRHVPFSEVP
                     VGGLDPERTQDWDIEDWVRAERSHFLAWLDLMKMAGQDLPPGPECPAAYDRVRQEILA
                     TMAEGAKDAAAEPEEPDPPPAPAPPGPRMGGEK"
     gene            5528..6007
                     /locus_tag="VGB57_13255"
     CDS             5528..6007
                     /locus_tag="VGB57_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132361.1"
                     /translation="MVTDTMPPSSTPRLATISTVISTAGAVVAAACAIISTNQVLKAV
                     QIDAQGKVFALKLDACDSFGETTATVSSDPLLAASDAAGRRKIVRSANRLIMLFPEDV
                     KSAGLKFINQIEIVYAARNGGPRDLPLNMALGSMMQAAIELEQACQSDIRKSAQGSP"
BASE COUNT          957 a         2134 c         2090 g          999 t
ORIGIN      
        1 cggaccgccg gcgccgaggc catcgtccat tatgtgaccg acctgtcctg cgcttcggcc
       61 ggccgctgca cggcgcgggg cggctatctc gaggggaatc tcagcgcgtc gggcaaccgc
      121 tatgagctgg agcgcaggaa cggccgctgg caggtcacgg cggacacgat ggaatggatc
      181 tcctgagcga aggccttgag cccgctcagt tcgaaaaggc gggacggtcc cgcgaggcct
      241 gatgaccctt tggacgcagc gacccggatc agccttttat gatgcacctg cttagactgc
      301 tgatgtcctg gtcggtccgg cgggtgcttg tgttcgttct gctggtcgcg gcgatggccg
      361 cgttcgtcca ggtcaggagc gcctatcagc gcctgcccga gctcaggcag caggtagagt
      421 cactcgagcg gcagcagggc ctgctcgaag cggaggtgga cgggcagcgg aagcaggcgg
      481 aggctcgcct tcaggcgatc ggaagcgccg aggagccggt gcttcgccgc cggctggccg
      541 atgtccgcgc cgcgatcgcg ggccaggagg gcggccaaag cggcggcctg ggcctcgccc
      601 tcgatgcggc gcgcggcgac ggcaatgcgg tcgccggcga gctggccggc cgtttcggat
      661 tgcagctcct gcgccgcgag gaagccgtca tcgtcgcccg cctggacgcg atccggagcg
      721 gcggccaggt gaacggcctc gtcgcgagga tcggcgccct cgattcgagg atccgggcgc
      781 tggagcggag cgtcgccgcg atcgagcgcc gatacccttt gctggcgcgg atcgagcggg
      841 tgccgggcgt caggcgcatc gagggcccgt ggcaggaatt gcgcgcggcg cggcaggagc
      901 tcgccgccgc caggtccgag cggcagcgcc tggcgcccct atacctgacg gcccgcggcg
      961 ccttcgacca agcgggcacc gcctacaaga agagccaggc ggcggttcgg gaacttccag
     1021 cgccgaccgg ggagctccgg cggcagatcg agcagaagcg cgcgcagctc gccggccact
     1081 gggcgagccg ggcctgggcg gcggtgaagc cggtgctggg ctgggcgctc tgggtgatgc
     1141 tgctcatcct ggtgactccc cctgcggtga aggccttctg gttcttcgcc gtcgccccgc
     1201 tcgcttcgcg cctgcgcccg atcaggatcg gccgccccgg ccgcggcgag ctgcgctggg
     1261 ccgccgagcg cctcgccgac ggcgaggaga cccgaacggg gagcgccgtc tcgcggcgcc
     1321 tgctcctccg gccgggcgag cgattgctcg tccgcccgga atatctgcaa agctcggtgg
     1381 tcggagcaaa agcggattcg cagctgctgc tgagccccgc tcttcccctc ggcagcctcg
     1441 ccaccggtct cgtcggtctc acccgcattc gccccgagca ggagaccagt gcggccattt
     1501 ccgccaccag ggacctgatc gacgagatcg gcctgctcga aatccccgaa ggctcggcaa
     1561 tcgtcttcag gccgcgcaac ctcgtcggaa tcgttctgac cggcagccag cccctgcggc
     1621 tcgaacgggt ttggagggcc gcctgcctca tgtcctggct gaccctccgg cttcggcacc
     1681 tcgtcttcca cggtccctgc gcgctggtgg tgaagggcgc ccgcggcgtc gcccttgagc
     1741 ccgccgccgg cggccggcga gtagccgatg ccgcgaccat gggctggagc gccggcctcg
     1801 actattcggt gacgcgaagc gaaaccttcc tcgcctatct caccggcaag cagagcctgt
     1861 tccacgaccg cttcgaaggc gagagcggcc aggtcgtcta cgagcagatg ccgcgggcga
     1921 gggcgaaatc cggcctcttc ggccgaggca tagagggcct cggcgacggc ctgctgaaaa
     1981 tcgccggcct ctgacctctc ctcaccgcgc cttcgcaaac cccatcgccg cgtcgcacgc
     2041 cagcttgtcc gcccgctcgt tctccggatg cccggaatgg cccttgaccc agcgccagtc
     2101 caccttgtgc ggggccgccg agtcgagcaa ttcctgccag agctcggcat tcttgaccgg
     2161 cttcctgtcc gccgtcttcc agccgttgcg gcgccagccg tggatccatt tggtgattcc
     2221 gtcgcgcacg tagatgctgt ccgtgtagag ggcgacctcg cagggccgct tcagggcctc
     2281 cagcgcgcgg atcgcggcga ggagctccat ccggttgttg gtggtcagcg gctcgccgcc
     2341 gctgatctcc ttctcgtgcg ggcccgaacg gattaccgcg ccccagccgc cggggccggg
     2401 gttgcccttg caggcgccgt ccgtgaagat ttcgacgatc gggacggtca agcggcttcg
     2461 agcccccggg gcagcttgaa caccatccgc tcgatcacgc cttcgccctc gagctcggtg
     2521 acgtcgtaat ggcggccgag gaagtcgacg acctcccgaa ccagcgtctc cggggccgag
     2581 gcgccggcgg tgatccccag ggtcgcgacc ccggccagcc agcccaaatc gatgtcgctc
     2641 gcccggccga tcagccgcgc cctcgtgccc tcgcgctccg cgacctcgac cagccgaacc
     2701 gagttggagc tcttgggcga tccgatcacc agcatcgcct cgcaggcgcg ggcgatgtcc
     2761 ttcaccgccg cctggcggtt cgaggtcgca tagcagatgt cctcgccctt gggcccccgg
     2821 atgccgggga agcggcgctc gaggatcgcg acgatctcgg ccgtgtcgtc gaccgaaagc
     2881 gtggtctggg tgaggtaggc gaggccgtcg ggatcggccg gagcgaccct ttgcgcatcc
     2941 tcgacgcttt cgatcagggt catccgtccc tccggcacct ggccgaaggt cccgatcacc
     3001 tcgggatggc cggcatggcc gatgaagagg atgtggcggt cgttctcgac cagccgctcg
     3061 gcctggcggt ggaccttgga gacgagcggg caggtggcgt cgagatattc gagcccgcgc
     3121 tcctgcgccc tggccggcac cgccttgggc actccgtgcg ccgagaaaac caccggaacc
     3181 ccgtccggaa cctggtcgag ctcgggcacg aatatcgccc ccttggcctt gaggctgtcg
     3241 acgacgaact tgttgtggac gatctcgtgg cggacataga cgggcgctcc gtagcgctcg
     3301 agcgccagct cgacgatccg gatcgcccgg tcgactccgg cgcagaagcc gcgcggggcc
     3361 gcgatcagca gggtgagggg gggcttggag gccatgtgac gagagctcta ctggatcgct
     3421 tgcctagacg gaacccggtt cctatgatcc gcggcgtccc gtccaaaggt gaggattccc
     3481 accctgtgtt caagtccaag gcggccctcg ccgcgcttat tcccctcggt ctcgccgcct
     3541 gctcgacccc gaacggccag atcaccgaca ccggcatcac cgccacccgc tccgcctgcc
     3601 cggcggtggc gatccccgcc gcgaccggcg acatcacttt gttcaacccg ccgagcagcc
     3661 gcgacgccgc ggcgatcgat gtggtcgcca acatcaccaa cctgcgctcg acctgcgacg
     3721 agagcggcgc caatatcgtc acgaccctga ccttcgacgt ccatgcccag cgccgcgacg
     3781 gccgcggcgc gcgcgaggtg gtgctccctt atttcgtggt cgcggtgcag ggcgggaccc
     3841 aggtccagtc caagagcgtg agccgggtcg gcctgagctt cgccgacggg gcgctgaggg
     3901 cgagcggccg aggctcggcg accggctcgg tgacccgggc ggcggtgacc ctgcccgccg
     3961 acatcaccgc ccagatcacc cgccgccgca agcccgggga tccgtcggcg gcgatcgatc
     4021 caatggccga tccgacggtg cgcgaggcgg tgcgccgggc gaccttcgag atgctggtcg
     4081 gcttccagct caccgaggag cagctcgcct acaacgcgac ccgctagtag gatcgacaat
     4141 tccactctcc cgcaaggacg ggagaggaag ctaatggatt ggctggccgc ccctgccctc
     4201 tcggcccttg ccgcagtcgg ccgccccgcg ttttgcgggc gaagttgaag aagttgaagc
     4261 tcgccatgag ccgtcatttc tctcctccca ttcgagggcc gggaggagcg ggcgcggggg
     4321 gcgggtcggg ctcttccggt tccgccgcgg cgtccttcgc cccctccgcc attgtggcaa
     4381 gtatttcctg gcggacccgg tcgtaggcgg ccgggcattc gggcccgggc ggcagatcct
     4441 gccccgccat cttcatcagg tcgagccagg ccagaaagtg gcttcgctcg gccctgaccc
     4501 aatcttcgat gtcccaatcc tgtgtccgct ccgggtccag cccgccgacc gggacctcgc
     4561 tgaacggcac gtggcgccag tcgctgcagc cgctgagacc ggccagggag tcgagactgt
     4621 cacgacggaa accgggccgg ccgctctcct cgggctcccg cggcctccgc gcctcgatga
     4681 aggcttcctc gtcgccgccc gcctcgacca cctccagata atcctcgaaa tcctcggaca
     4741 gggtgcgaag tagctccgct tcctcgctgt ccttgctttc ggcgagccgg tcgaggcggg
     4801 taagcatggc catggacagc cggttgtcga agcggcggcg ctcggcgatc accacgccgt
     4861 ccttgaggac ctgctcgacg cagccgttca tcgcccggga cagattgttg tcggcgagcc
     4921 ggttgcgggc gcggtggatc agaatcgcgt cccacatctt gccgaaggcg cggcccgagc
     4981 ggcggttctt cagcgcatag gcgctggcga cgctcatccc gaccgaccgg caggcgtcgg
     5041 cgacgacccc gccagcgacg aggacgagga ggaaccgcgc cttcttccag gggagccagc
     5101 catcgacgcg gaggcggcgg ccggacgggt cgaatacctc cgccgcctcg ccgcggccgt
     5161 cgcgcggcgc gaccggctcg tcgtccaccc gcgaattctc gccggtcgcg caacgcaggc
     5221 cgtgagagac gcccgcccac caggcgtcgg cctccgcctg agtggcgaat tgcgcgtcgt
     5281 cggcagggtg cgcgctcagc tcgccggcag cgcgagggga ggggtcggcc cgctcgtcga
     5341 tcgatgcttg agcagagatg ttgctattat gttccatact gaagttagat caaaacatct
     5401 cctgcatttc tacttcaatt ttgaggagct tcgattggcg caaaagcaat gttcatcaaa
     5461 gactcgtttc cttggcgaag caggcccaat caaataggat gagccaaacg ggagggggcg
     5521 ccaaactatg gtaaccgaca caatgccgcc gtcgtcgaca ccaaggctcg cgacgatcag
     5581 cacagtgatc tcgactgcgg gcgctgtcgt cgcagccgcc tgcgccataa tcagcacgaa
     5641 ccaagtgctc aaggccgttc agattgacgc acagggcaaa gtgtttgcgc tgaagttgga
     5701 tgcgtgtgac tcattcggcg agacgacagc gaccgtctcg agcgatcctt tgcttgccgc
     5761 ttccgatgcc gcgggccggc ggaaaatagt gcgcagcgcc aaccgcctca tcatgctgtt
     5821 tccggaagac gttaagagtg cagggctgaa attcatcaat cagatcgaaa tcgtctacgc
     5881 tgcgcggaac ggagggccgc gggaccttcc cttgaacatg gcgctgggat cgatgatgca
     5941 ggcggctatc gagctagagc aggcgtgcca aagcgacatc aggaagagcg cgcagggttc
     6001 cccatagcgt agaaacacca gggaaaatcc tcggtgcctg gcaccgggga aggccgaccg
     6061 ctgctcggat gacgtcgatc taaatcctcc cccggttttg caggggggga ctcgaagccg
     6121 ggtggagggg gacttgctga gttcgaagaa cccctccacc gcgcttcgcg cggtccccct
//
LOCUS       DASWNU010000657         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_207385, whole genome shotgun sequence.
ACCESSION   DASWNU010000657 DASWNU010000000
VERSION     DASWNU010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207385"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VGB57_13260"
     CDS             complement(<1..584)
                     /locus_tag="VGB57_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132362.1"
                     /translation="MSAPEYIPFGLTALFIAVLVWSFLPSKQPEPVTGTPALPPPPFA
                     DEHFGERIIRAIADGVNVRPGSGSDIGDRIDDKEREAREYQAVLAKQRAVITAKAQLM
                     TDRLASRNHSMMEPGRSPTHDSYLAAMWDAEERGEMLDETHRSTFPPPPPNRWELLSR
                     AAKLMGVFEMPLRDVVRRVAFESKWAIRYDRTNAL"
     gene            complement(834..1355)
                     /locus_tag="VGB57_13265"
     CDS             complement(834..1355)
                     /locus_tag="VGB57_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0132363.1"
                     /translation="MTDGSDTTLLIAILVAALAAAIVAGLVWRGARRRRRNRAPVLSE
                     RSGNERPYVRPPPTPPPAHAGVADEVAAATTDVLRDVLSVEKEAAAGSDDLQRLKGVG
                     PKLATRLRELGITSYAQLAHLGETEVARLDERLGPFSGRVSRDRLVEQAQFLARGDTD
                     GFEARFGKLGGEG"
     gene            complement(1462..>1700)
                     /locus_tag="VGB57_13270"
     CDS             complement(1462..>1700)
                     /locus_tag="VGB57_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132364.1"
                     /translation="SYQKEGMGSYPGINDRMRTIPGMAGIRWQGEQGDRVLPLLAEIL
                     LLAAAAWLIGLGIGWLLFGRKKRQSFLGDEEFET"
BASE COUNT          275 a          543 c          589 g          293 t
ORIGIN      
        1 agcgcatttg tccggtcgta tcggatcgcc cacttgctct cgaacgcgac acgccgaacg
       61 acatcgcgca acggcatttc gaaaacgccc ataagcttgg ccgccctgga gagcagttcc
      121 catcgattcg gcggcggagg cgggaatgtc gagcggtgcg tctcgtccaa catctcgccc
      181 cgttcttccg cgtcccacat tgcggcgaga tatgagtcgt gtgtcgggct tcggcccggc
      241 tccatcatgc tgtgattccg cgaagcaagg cggtctgtca tcaattgcgc tttggctgtg
      301 atgacggccc tctgcttcgc caagaccgcc tgatattccc ttgcctcccg ctccttgtca
      361 tcaattcgat ccccgatgtc gctgccagag ccggggcgga cgtttacgcc gtccgcaatt
      421 gcgcgaatta tccgctcgcc gaagtgctca tcggcgaagg gcgggggtgg gagggcggga
      481 gtgccggtga caggctcggg ctgcttggac ggcaaaaaac tccaaacgag cacggcaatg
      541 aacagagccg taagtccgaa cggaatgtac tctggcgcgc tcacgatggc tttccacgca
      601 tcgctggcat cggttggaag gttggcaacc ccgataactg tagtaaccac cgcccaggcg
      661 gtggcgacgg ccgcgccgat cccggtggtg atagcaccct ccggcttttt cgcttttgcc
      721 tgacagcctc tggaagcagc cccatcgggg ctctatacca ctactggaag ccctttggcg
      781 caaggggata aacgccgatc aagttcgggg tgacggcctt tcggcgagcc ggattatccc
      841 tcgccgccca atttgccgaa gcgcgcctcg aagccgtcgg tgtcgccgcg ggcgaggaat
      901 tgcgcctgct cgacgagccg gtcgcgggag acgcggccgc tgaacgggcc gagccgctcg
      961 tccagccggg cgacctcggt ctcgccgaga tgggcgagct gggcgtagct ggtgatgccg
     1021 agctcgcgca gccgcgtcgc aagcttcggc ccgacgcctt tcaggcgctg gagatcgtcg
     1081 gaccccgcgg ccgcctcctt ctcgacgctg aggacgtcgc gaagcacgtc ggtcgtcgcg
     1141 gcggcgacct cgtcggcgac gccggcatga gcgggcgggg gcgtcggcgg ggggcggaca
     1201 taggggcgct cgttgccgga gcgttcgctg agcaccggcg cgcggttacg gcggcggcgg
     1261 cgggcgccgc gccagaccag cccggcgacg atcgccgcgg ccagcgcggc gaccaggatg
     1321 gcgatcagga gggtcgtgtc ggagccgtcg gtcatgtctt gtcgcgtcct gaaggcgggc
     1381 ggcgaaaggt ccagcgagcg gtggcgatgc cgatcagcaa cgccgcggcg agcgggaaga
     1441 aattggcctg gatgagggcg gtcacgtctc gaactcctcg tcgccgagga agctctgccg
     1501 cttctttcgg ccgaacaaca gccagccgat gccgaggccg atcagccagg cggcggcggc
     1561 gaggaggagg atctcggcga gcagcggcag gacgcggtcg ccttgttccc cctgccagcg
     1621 aatgccggcc atgcccggga tggtgcgcat gcggtcgttg atccccggat aggagcccat
     1681 tccctccttt tggtaggaat
//
LOCUS       DASWNU010000658         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_466105, whole genome shotgun sequence.
ACCESSION   DASWNU010000658 DASWNU010000000
VERSION     DASWNU010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466105"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VGB57_13275"
     CDS             <1..1110
                     /locus_tag="VGB57_13275"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132365.1"
                     /translation="EASATGEDAIAVGALSEASLDGAVALGFDADATSFNAIALGTGS
                     RATGIGAIAGGVGAEASGENSIATGQYTTASGASSIAQGDDVTASGENSVAISTLAQA
                     TGEGSIAIGNRSVSSAQRTVALGDSAIASGEDAIALGALAEATTDGALAVGLNARANG
                     VNASAFGTDANAIQNGSTAVGSGATTTAANQVALGGNSSSVRIGDIAASTAAQTGTIG
                     VATVDASGTLGRNTTIIPAISSLQAGQATQAGQIVALQGVQASQGAQILGLQGAQAAL
                     SSSVDTLFDLRRIDRRDSRQGIAAAVAMGHAAMPSEPGRTSYVVNGATYRGELAIGAS
                     VAHRMRGNNPFAITAGFSYAGNKNNAARVGVAGEF"
     gene            1281..2108
                     /locus_tag="VGB57_13280"
     CDS             1281..2108
                     /locus_tag="VGB57_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132366.1"
                     /translation="MRNPARKALYCLLLVLASAGLIAIGAEREQRLGEDWTALGPILL
                     GFALLPFPLVFLVQALFAIRGRAKLLAGIGVIARWQVDPIEWGRFRGLDQRRSAQDFS
                     LGSDLWIRRRAPAEPVQVIVGEKSALVDGSYHSLRPGGLPELRAVSWLEGPPTCLEFA
                     LRYPRGRYGGTVPMTLRIPVAASARGAALRVFAHFERVTRRSPGLALRNPPRTYRICA
                     ALVAGAAVTGGVGYAIAGAVADGHGQDVLLGLLIGATILAVFAVILALATFLLTRKT"
     gene            2105..2557
                     /locus_tag="VGB57_13285"
     CDS             2105..2557
                     /locus_tag="VGB57_13285"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0132367.1"
                     /translation="MRIRAARPADVEAMHALRSRVAENALSDPRHVTPESYFPYLAQG
                     QAWVAETGRGLAGFAILDVAGASVWALFVAPEAEGKGVGRALHARLIEGAIGHGLTRL
                     SLSTAPRTRAERFYSEAGWTRTGETRTGDLSFGKDLAGEAMTSEGGQG"
BASE COUNT          389 a          925 c          928 g          377 t
ORIGIN      
        1 gaggccagtg cgacgggcga agacgccatc gcggtcggtg cactgtccga agccagcctc
       61 gacggcgccg tggcgctcgg cttcgacgcg gacgccacca gcttcaatgc gatcgccctc
      121 ggcacgggct cgcgggcgac cgggatcgga gcgatcgcgg gcggcgtcgg cgccgaggcc
      181 agcggcgaga acagcatcgc caccggccaa tatacgaccg cttccggagc ctcctcgatc
      241 gcccaaggcg acgatgtgac ggcgtcgggc gagaacagcg tcgccatctc gaccctcgcg
      301 caggcgacgg gggaaggaag catcgccatc ggcaacagga gcgtttcgag cgctcagcgg
      361 acggtcgccc tgggcgacag cgcgatcgcg tccggcgagg acgcaatcgc tctcggcgcc
      421 ctggccgagg cgaccacgga cggcgctttg gcggtgggcc tcaacgcccg ggcgaacggc
      481 gtcaacgcgt cggccttcgg aaccgacgcg aatgcgatcc agaacggatc gacggcggtc
      541 ggctccggcg ccacgacgac ggcggccaac caggtggcgc tgggcggcaa ctcaagctcg
      601 gtgcgcatcg gcgacatcgc ggcgagcacc gccgcgcaga ccggaacgat cggagtcgcg
      661 accgtcgacg cgtcgggaac gctcggccgc aacacgacga tcatcccggc catctcgtcg
      721 cttcaggccg gccaggcgac gcaggctggc cagatcgtcg cgcttcaggg cgtccaggcc
      781 agccagggcg cccagatcct gggactgcaa ggcgcccagg ccgcgctctc ctcgagcgtc
      841 gataccctgt tcgacctcag gcgcatcgac cggcgggact cgcgccaggg gatcgccgcg
      901 gcggtcgcca tgggccatgc ggcgatgccc tcggagcccg gccgcaccag ctacgtcgtc
      961 aacggcgcca cctatcgcgg cgagctggcg atcggagcct ccgtcgcgca ccggatgcgc
     1021 ggcaacaacc cgttcgcgat caccgccggc ttctcctatg ccggcaacaa gaacaacgcc
     1081 gcgcgggtgg gcgtcgccgg ggagttctag gcggaatcga gcagtggacg gacgacctgc
     1141 gtcattcccg cgttcgcggg aatgacgcag ggggcgccct gccacacggc tcgaaacccc
     1201 gacaggaccg ctttaccttc cgcttgcgct cggcgatgct ctcatcgttc gaggggaagc
     1261 cggaggggag aaaggtcggg atgcgcaatc cggcgcgcaa ggcgctttac tgcctgcttc
     1321 tcgtcctcgc ttccgcgggg ctgatcgcga tcggcgccga acgcgagcaa cgtctcggcg
     1381 aggattggac cgcgctcggc ccgatcctcc tcggcttcgc cctcctgccc ttccctctgg
     1441 tcttcctggt ccaggctttg ttcgcgatcc gaggccgggc caagctgctc gccgggatcg
     1501 gagtgatcgc ccgctggcag gtcgatccga tcgaatgggg gcggttccgg gggctcgacc
     1561 agcgccgctc ggcgcaggat ttctcgctcg gcagcgacct gtggatccga aggcgggccc
     1621 cggcggagcc ggtccaggtg atcgtgggcg agaagtcggc gctggtcgac ggcagctatc
     1681 attcgcttcg gccgggcggg cttcccgagc tgcgcgcggt gagctggctg gagggaccgc
     1741 ccacctgcct cgaattcgcg ctccgttatc cccgcggccg ctatggcggg accgttccga
     1801 tgacccttcg aatcccggtg gccgcgagcg cgcgcggggc ggcgcttcgg gtgttcgccc
     1861 atttcgagcg ggtcacccgc cggagccccg gcctcgccct gcgcaatccg ccgaggacct
     1921 acaggatctg cgcggcgctg gtcgccggcg ccgccgtgac gggcggcgtc ggctatgcca
     1981 tcgccggcgc cgtcgccgac ggccatggcc aggacgtgct gctcggcctc ctcatcggtg
     2041 cgaccatcct cgcggtcttc gccgtcatcc tcgctctggc gaccttcctc ctgacccgga
     2101 agacatgagg atcagggccg cccgacccgc cgacgtcgag gccatgcacg cgcttcgaag
     2161 ccgggtcgcc gagaacgcct tgtcggatcc ccggcacgtc accccggaat cctatttccc
     2221 ctatctcgcc cagggccagg cctgggttgc cgagaccggg cggggccttg ccggcttcgc
     2281 catcctcgac gtcgccggcg ccagcgtctg ggccctgttc gtcgctccgg aggcggaggg
     2341 gaaaggggtc ggccgggcgc tccacgccag gctgatcgaa ggagcgatcg ggcacggcct
     2401 cacccggctt tccctaagca ccgccccccg cacccgggcg gagcgcttct atagcgaggc
     2461 gggctggact aggaccggcg agactcgaac gggggacttg tcgttcggga aggatctggc
     2521 cggtgaggca atgacaagcg aaggaggaca ggggtgatgc aacgatgccg gtctgcggcg
     2581 gttgggcgcc gcatgagaag aacgatcctc gccgcggcg
//
LOCUS       DASWNU010000659         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_466161, whole genome shotgun sequence.
ACCESSION   DASWNU010000659 DASWNU010000000
VERSION     DASWNU010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466161"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /gene="rpsT"
                     /locus_tag="VGB57_13290"
     CDS             <1..183
                     /gene="rpsT"
                     /locus_tag="VGB57_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0132368.1"
                     /translation="IRTFVKQVEAAIEAGDKDQALAAISRVQPELARGVAKGVFHKNT
                     AARKFSRLTRRIAALA"
     gene            623..>1674
                     /gene="dnaA"
                     /locus_tag="VGB57_13295"
     CDS             623..>1674
                     /gene="dnaA"
                     /locus_tag="VGB57_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY0132369.1"
                     /translation="MERGALQSAWEAIRTGLRRDCGARTFDGWLRPIALGAFDSESGG
                     LRLELPSQFMADWVQTHFSERLTLAWRAACPAVRHISIRVGAAAPKTAAVVEIPAPPA
                     SEEPLPGDGLYAVALEPRYRFENFVVGKANEVAFSAATTLATADNVAFNPLFLHGGTG
                     RGKTHLLHAIGHEFHRLRPRAKIIYMSAEKFMVEFVAALRANDTIKFKQHLRAADLLM
                     IDDVQFIAGKESTQEEFFHTMNEIISAGRRLVISSDRAPQDLDGIEPRILSRLSWGLV
                     ADINAADLELRLNIIHKKLESLPDVKVPEDVIGFLARRISSNVRELEGALNRISAYSM
                     MQNRPITLEFVSEVLA"
BASE COUNT          296 a          591 c          495 g          292 t
ORIGIN      
        1 atccgcacct tcgtcaagca ggtcgaggcc gcgatcgagg ccggcgacaa ggatcaggcg
       61 ctcgccgcca tctcgcgcgt ccagccggag ctcgcccgcg gggtcgccaa gggggtgttc
      121 cacaagaaca ccgcggcccg caaattctcg cgactgacgc gccgtatcgc cgctctcgcc
      181 taagcccttt cagacgcttc aaagtcgccc gccgggaccc gtttccggcg ggctttttcg
      241 tgtcattttc ctgtcattgg aacgtaacac gaaaccctag gatttcccgc gattcgcgcg
      301 cctgccgatt tgaagaaatg gcggttttcc gccattttaa cggcgggccg tgcaaaacct
      361 atgtcaagca tgtttctttg aactgacacc aaatttgcgc cttgcaaggc cgggccgaga
      421 cttccaaaaa ggtctctccg gcggggcgaa tctccctccg gacgccacag caagaacgat
      481 ctttcgaatc ggcggccgcc ggggggtggc cgcgtgccgg aaggagtttg cgccagtcag
      541 ggcccgattg aaagaggcct ggtaggaaga gggggtgcgt ttcgtgtctg tgaagcccgc
      601 gggaggcagt tcgtcgaatg ggatggagcg gggcgcgctc cagtcggcct gggaagcgat
      661 tcgcaccggc ctccggcgcg attgcggcgc ccgtactttc gacggctggc tccgcccgat
      721 cgcgctgggc gccttcgact ccgaatccgg cggtcttcga ctcgagctgc cctcgcaatt
      781 catggccgac tgggtccaga cccatttctc cgagcgcctg accctcgcgt ggcgcgccgc
      841 ctgcccggcc gtccgccaca tctcgatccg ggtcggcgcc gccgcgccca agaccgccgc
      901 ggtcgtcgag atccccgcgc cgccggcctc ggaagagccc ctccccggcg acgggctcta
      961 tgcggtcgcc ctggagcccc gctaccggtt cgagaatttc gtcgtcggaa aggccaacga
     1021 ggtcgccttc agcgcggcca ccacgctcgc caccgccgac aatgtcgcct tcaacccgct
     1081 cttcctccac ggcggcaccg gccgcggcaa gacccacctt ctccacgcca tcggccacga
     1141 attccaccgc ctgaggccgc gcgccaagat catctacatg tcggccgaga agttcatggt
     1201 cgagttcgtc gccgcgctcc gcgccaacga cacgatcaag ttcaagcagc atctgcgcgc
     1261 cgcagacctc ctcatgatcg acgacgtcca gttcatcgcc ggcaaggaat cgacccagga
     1321 agaattcttc cacacgatga acgagatcat ctcggccggc cgtcgactcg tcatcagctc
     1381 ggatcgcgcc ccgcaggacc tcgacggaat cgagccgcga atcctctcgc gcctgtcctg
     1441 gggcctggtc gccgacatca acgcggccga cctcgagctg cggctcaaca tcatccacaa
     1501 gaagctcgaa tcgcttccgg acgtgaaggt gcccgaggac gtgatcggct tcctcgcccg
     1561 ccgcatcagc tccaacgtgc gcgaactcga aggcgcgctg aaccgcatct cggcttattc
     1621 gatgatgcag aaccggccga taacgctgga attcgtgtcc gaagtgctcg ccaa
//
LOCUS       DASWNU010000660         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_207554, whole genome shotgun sequence.
ACCESSION   DASWNU010000660 DASWNU010000000
VERSION     DASWNU010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207554"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VGB57_13300"
     CDS             <1..1502
                     /locus_tag="VGB57_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0132370.1"
                     /translation="TGEEKSVQLIAERFRQAGLQPGNKGSWYQNVPLVESLATPTALT
                     LSGGGAPMTFAHRTDFVANSYQVQSRIDLQGSEMVFVGYGINAPERGWNDYAGVDVKG
                     KTVVILVNDPDWQDSDLEGTFNGKAMTYYGRWTYKYEEAARQGAAAAFIVHDPEPAAY
                     GWNVVQSSWTGPQYNMDEPGGHMDQSKVIGWLTNEAARRILAASGQDLTALSAAARRP
                     GFKAVPLRTRASIGLTNQIKKQASKNVIGILPGSKRPNEYVIYTAHWDHLGRCDADKT
                     GDDICNGALDNATGTAGLIALAEAHARRGNPERSIVFLAVTAEESGLLGSRYYAENPV
                     YPLASTAGGVNMDGLNVIGDTRNFVVIGKGKSQLEDYMKRATDRYGLRVENEPSPQAG
                     YYYRSDHFSLARQGVPMLYAEGGEDLVEGGAAAGHAAAEDYRANRYHQPSDEYDPKWN
                     WSGAIRDLEIYWLIGRELADSSDWPNWNPGDEFRAIRDRSRGNAGPIMR"
BASE COUNT          309 a          534 c          524 g          238 t
ORIGIN      
        1 cgacgggcga ggaaaaatcg gtgcagctga ttgccgagcg tttccggcag gcggggctcc
       61 agccgggcaa caagggcagc tggtaccaga acgtcccgct ggtcgagagc ctggccactc
      121 cgaccgcgct gacgctatcc ggcggcggcg ctccgatgac cttcgcccac cgcaccgact
      181 tcgtcgccaa cagctaccag gtccagtcca ggatcgatct ccagggcagc gagatggtct
      241 tcgtcggcta cggcatcaac gcgcccgagc gcggctggaa cgattatgcc ggcgtcgacg
      301 tgaaggggaa gacggtcgtg atcctggtca acgatcccga ctggcaggac agcgacctcg
      361 aggggacgtt caacggcaag gcgatgacct attacggtcg ctggacctac aaatatgagg
      421 aagcggcccg ccagggcgcc gcagccgcct tcatcgtcca cgatccggag ccggccgcct
      481 acggctggaa cgtcgtccaa tcctcctgga ccggcccgca atataatatg gacgagcctg
      541 gcgggcacat ggaccagtcg aaggtgatcg gctggctgac aaacgaggcg gcgcggcgga
      601 tcctggcggc ttccggccag gacctgacgg cgctctccgc cgcggccagg cgccccggct
      661 tcaaggccgt gcccctgcgc acccgcgcgt cgatcggcct taccaaccag atcaagaagc
      721 aggcgtcgaa gaacgtgatc gggatccttc ccggctcgaa gcgccccaac gaatatgtga
      781 tctacaccgc ccactgggac catctcggcc gctgcgacgc cgacaaaacg ggcgacgaca
      841 tctgcaacgg cgccctcgac aacgcgaccg gcaccgccgg cctgatcgcg ctcgccgagg
      901 cccatgcgag gagaggcaat cccgagcgct cgatcgtctt cctggcggtc acggcggagg
      961 aatcggggct gctcggctcc agatattatg ccgaaaaccc ggtctacccg ctggcgagca
     1021 ccgccggggg cgtgaacatg gatgggctca acgtgatcgg cgacactcgg aacttcgtcg
     1081 tcatcggcaa gggcaagtcg cagctcgaag actatatgaa gcgcgccacc gaccgctacg
     1141 gcctccgggt cgagaacgag ccctcgccgc aggcgggcta ttattaccgc tccgaccatt
     1201 tcagcctcgc tcgccagggc gtgccgatgc tctacgccga gggcggcgag gatctggtcg
     1261 agggcggagc cgccgccggc catgccgcgg ccgaggatta ccgcgccaac cgctaccacc
     1321 agcccagcga cgaatacgac ccgaagtgga actggtcggg cgcgatccgc gacctcgaaa
     1381 tctattggct gatcggccgc gagctggcgg attcctcgga ctggccgaac tggaatccag
     1441 gggacgaatt ccgtgccatc cgcgaccgca gccggggcaa tgccggcccg atcatgcggt
     1501 gagggtttga agcgagggtc gggctgacaa ggcaaaccgg tttgcgagcc gtcggttcgg
     1561 ctcccatgag agttcaagcg tatcggcctg agttcgaagc ctctt
//
LOCUS       DASWNU010000661         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_129733, whole genome shotgun sequence.
ACCESSION   DASWNU010000661 DASWNU010000000
VERSION     DASWNU010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129733"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2203
                     /locus_tag="VGB57_13305"
     CDS             <1..2203
                     /locus_tag="VGB57_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0132371.1"
                     /translation="RVSDTRVVIDAVHPQVEVAPDRFRTRMPRWAGVSPDGRQIVFET
                     LGKLWIKPTAGGAPRRLVKGNEEELELFPAWSRDGRTIAYVGWTDRGLGRIRTVAAGG
                     GTPRQVTAQPGHYARPQFSPDGKTIVFEKGQGGFLTSARWSENPGIYRVPAAGGTPAR
                     VSAEGAAPQFAASNDRLFMIANAAGKRQLVSTDLNGEAKHVHSTGEMVSDYQVSPDGQ
                     YLAFRQNYQAFVMPLMPGTQDVAADAKGGPLPVTKVSAVGAEFIHWSGGGRRLHWSTG
                     PNVYTVDAATLFGSAPPAENAPKFQPPATGLSLSMEVEADRPSGIVALTGARIVTMAA
                     ADGGIIDDGVIVVRGDRILAIGPRGQVSIPAGAKTVDVTGKTIIPGLVDAHAHGPQGQ
                     DDLVPQQNWAQIANLAMGTTTAHDPSARAAEIFAAAEMQRAGKILSPRTFSTGEIVYG
                     AKAADVYAEINTFEDALAHVRRLKAQGAHSVKNYNQPRRDQRQMVVAAGQREGMEVVP
                     EGGSLFTMDISLIQDGNATVEHNVPAEIFYDDVVSLWTQSKTNYTPTLVVAYGGLAGD
                     PYWRQHMDVWKHPLLSRHAPPTLLAAQNVRRTMAPEEDFVDDDAAREAKKLADRGVQV
                     AIGAHGQQAGLGPHWEMWSFVRGGWSPIEALRAGTIAAATSLGYSRDVGSLEAGKLAD
                     LVVLDADPTADIRNSDKVHRVMLGGRLYDPVTMNEVVTGTRVRAPYWWEEN"
     gene            complement(2317..>2824)
                     /locus_tag="VGB57_13310"
     CDS             complement(2317..>2824)
                     /locus_tag="VGB57_13310"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0132372.1"
                     /translation="IDKLPNNWVHVGIIHLILPNAKVIDARRHPLGCCFSNFKQHFAH
                     GQAFTYSLADVGRYYSDYVRLLAHFDSVLPGRIHRVFHERMVDDSETEIRRLLEYLGL
                     PFEEACLRFYENDRAVRTPSAEQVRRPINREGVERWKAYEPWLGPLKEALGPVLTCYP
                     QVPEDWAD"
BASE COUNT          473 a          949 c          975 g          427 t
ORIGIN      
        1 ccgggtcagc gacacgcgcg tcgtcatcga cgccgttcat ccgcaggtgg aggtcgcccc
       61 cgatcgcttc cggaccagga tgccgcgctg ggcgggcgtt tcgcccgacg gccgccagat
      121 cgtgttcgag accctcggca agctgtggat caagccgacc gccggcggag cgcctcggcg
      181 gctggtgaag ggcaatgagg aggagctcga gctcttcccg gcctggtcgc gcgacggccg
      241 gacgatcgcc tatgtcggct ggaccgaccg gggcctggga cggatccgaa ccgtcgccgc
      301 gggaggcggg acgccgcgcc aggtgacggc tcagcccggc cattatgcgc ggccgcaatt
      361 ctcgcccgac ggcaagacga tcgtcttcga gaaagggcag gggggcttcc tcacctccgc
      421 ccgctggtcg gagaatccgg gcatctaccg ggtccctgcc gccggcggaa ctccggcgcg
      481 ggtctccgcg gaaggcgccg caccgcaatt cgcggcttcc aacgaccgat tgttcatgat
      541 cgccaacgcc gcgggcaagc gccagctggt cagcaccgat ctgaacggcg aggcgaagca
      601 cgtccattcg accggcgaga tggtcagcga ctatcaggtc tcgcccgacg gccagtatct
      661 cgccttccgg cagaattatc aggcgttcgt gatgcctctc atgcccggca cgcaggatgt
      721 ggcggcggac gcgaaaggcg gcccgttgcc ggtgacgaag gtcagcgcag tcggagccga
      781 gttcatccac tggtccggcg gcggccgccg gcttcactgg agcaccggcc ccaatgtcta
      841 cacggtcgat gccgccacct tgttcggctc cgctccgccg gccgagaatg caccgaagtt
      901 ccagccgccg gccaccgggc tttcgctgtc gatggaagtc gaggcggatc ggccgagcgg
      961 catcgtcgct ctcaccggag cgcggatcgt cacaatggcc gcggccgacg gcgggatcat
     1021 cgacgacggg gtcatcgtcg ttcggggcga ccggatcctg gccatcgggc cgcgcgggca
     1081 ggtgagcatc ccggccgggg cgaagacggt cgacgtcacg ggcaagacga tcatcccggg
     1141 cctggtcgat gcccatgccc acggccccca gggccaggac gacctcgtcc cgcagcagaa
     1201 ttgggcgcag atcgccaacc tcgccatggg gacgacgacc gcgcacgacc cgtcggcgcg
     1261 ggcggccgag atcttcgccg ccgccgagat gcagcgcgcc ggcaagatcc tctcgccccg
     1321 caccttctcg accggtgaga tcgtctacgg cgccaaggcc gccgacgtct acgcggagat
     1381 caacacgttc gaggacgcgc tcgcccacgt ccggcggctt aaggcgcagg gcgcccacag
     1441 cgtcaagaat tacaaccagc cccggcgcga ccagaggcag atggtcgttg ccgccggtca
     1501 gcgcgaaggg atggaggtcg tacccgaagg cggatcgctc ttcacgatgg acatctcgct
     1561 catccaggac ggcaacgcga cggtcgagca caacgtgccg gcagagattt tctacgacga
     1621 cgtcgtcagc ctgtggaccc agtccaagac caactacacg cccaccctcg tcgttgctta
     1681 tggcggcctc gccggcgacc cctattggcg ccagcatatg gacgtctgga agcatccctt
     1741 gctgtcgagg cacgcgcccc cgaccttgct cgccgctcag aacgtccggc gtacgatggc
     1801 gccggaggag gatttcgtgg acgacgacgc cgcccgcgag gccaagaagc tcgccgaccg
     1861 cggagtccag gtcgcgatcg gggcccacgg ccagcaggcc gggctggggc cgcattggga
     1921 gatgtggtcg ttcgtccgcg gcggctggag cccgatcgag gcgttgcgcg ccggaactat
     1981 cgccgcggcg acctcattgg gctattcccg cgacgtcggc tcgctcgaag ccggcaagct
     2041 cgccgacctg gtcgtgctcg atgcggatcc gacggcggac atccgcaaca gcgacaaggt
     2101 gcacagggtc atgctcggcg gacggctcta cgatccggtg acgatgaacg aggtcgtgac
     2161 cggcacccgc gtccgggcgc cttattggtg ggaagagaat tgattcgtcg tcccggcgaa
     2221 ggccgggatc ccagcgggtg aaaggcaccg cgcccttcac ccaccggggc ccaagcctgc
     2281 gctggggcga cggcctgagg gcggggtgcc ccgcggctag tccgcccaat cctccggcac
     2341 ttgcgggtag caggtcagca ccggtcccag cgcctccttg agcgggccga gccagggttc
     2401 gtaggccttc caccgctcga cgccctcgcg gttgatcggc cttcggacct gctcggcgct
     2461 gggcgtccgg acggcccggt cgttctcgta gaagcggagg caggcctctt cgaagggcag
     2521 gcccagatat tcgagcaggc gccggatctc ggtctcgctg tcgtcgacca tgcgctcgtg
     2581 gaatacccgg tggattcggc ccggcaggac cgaatcgaaa tgggcgagga gcctcacata
     2641 atcgctgtaa tagcggccga cgtcggccag ggaataggtg aaggcctggc cgtgggcgaa
     2701 atgctgcttg aagttcgaga agcagcagcc gagcggatga cggcgggcgt cgatcacctt
     2761 cgcattgggc aggatcaggt ggatgattcc gacatggacc caattgttgg gcaatttgtc
     2821 gatg
//
LOCUS       DASWNU010000662         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_207569, whole genome shotgun sequence.
ACCESSION   DASWNU010000662 DASWNU010000000
VERSION     DASWNU010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207569"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VGB57_13315"
     CDS             complement(<1..728)
                     /locus_tag="VGB57_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0132373.1"
                     /translation="MDDRDSLARLTEREKLCLRQWLQHKSAKEIAAGLGISHHAVEKR
                     LKMARTKLGATSSLEAARMLGEAEGYGRAVTQSPDLVSDALPLHSAFTRPTILGVAIM
                     ILVGAIVLGLLLQPATFSQAADNQPVAPPGERVSAPGSVPTAGTEAALRRLVAGLASG
                     SPDYDTLSPRFAEVVRRDLPMTHRLFSSMGELKSVTFRERGPMSDDVYNLVFANGGVT
                     MSAALDADGRMAGGMIQPAGQRGR"
     gene            940..>1818
                     /locus_tag="VGB57_13320"
     CDS             940..>1818
                     /locus_tag="VGB57_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0132374.1"
                     /translation="MTRGESNITATIAADAAGQRLDRALAAALPTLSRERLKALISSG
                     RVSGPAGLVRDPAAKAMAGVYQVVVPEPAPAHNEAQDIALEIVFEDDHLLVVDKPAGM
                     VVHPAAGNFDGTMVNALLHHCAGRLSGIGGVARPGIVHRIDKGTSGLLVVAKTDRAHE
                     GLAAQFARHSIDRRYLALVAGIPSPAAGTIDAPLARSSANRQKMAIVEGNRGKRAVTR
                     YRLLRPLCDSALVECRLETGRTHQVRVHMTSIGNPLLGDPAYGRTRSSHRELLKRLGF
                     ERQALHAAELGFIHPVT"
BASE COUNT          301 a          612 c          609 g          296 t
ORIGIN      
        1 cagcgccccc gttgccccgc cggttggatc atgccgccgg ccatcctgcc gtcggcgtcg
       61 agcgcggccg acattgtcac cccgccgttg gcaaacacca aattgtagac gtcgtcgctc
      121 atcggcccgc gctcgcggaa agtcaccgac ttcagctcgc ccatcgagct gaacaggcga
      181 tgggtcatgg gcaggtcccg gcggacgact tcggcgaacc gcggagagag cgtgtcgtag
      241 tcgggcgagc cgctcgccag cccggcgaca agcctccgca aggcagcctc ggtgccggcg
      301 gtcggcaccg agccgggggc ggatacccgt tctccaggcg gcgccaccgg ctggttgtca
      361 gccgcttgcg agaaggtcgc gggctgcaaa agcaacccga gcacaattgc tccaaccagg
      421 atcatgatgg ccactccgag gattgttgga cgagtgaagg ctgagtgtag cgggagagca
      481 tcggaaacca ggtccggcga ttgggttacc gctcgaccgt acccttcggc ttcgccgagc
      541 atccgagcgg cttccaggga cgacgtcgcg ccaagcttgg tgcgcgccat cttgaggcgc
      601 ttctcgaccg catggtgcga aatgccgaga cccgcggcga tttccttggc ggacttatgc
      661 tgcagccatt gccgcaggca gagcttctcg cgctccgtca atctcgcaag tgagtcgcgg
      721 tcgtccatcg atgaacggat aatggctgtc gccgctcgtc gatagtacgg tccgctggta
      781 aaacgggccg gacggcgttc atggcgtggc cgcaatgggc cggaagccgc cgcaacgaag
      841 gacagttcac ggccagaagc cgccattggg gtcacaagcc gattgctggc tcttccggcg
      901 gcttgaattc gccgcgcgcc gcgccacatc ggggccggaa tgacccgggg ggagtcgaac
      961 atcaccgcaa cgatcgccgc cgatgccgcc ggtcagcgcc tcgaccgggc gcttgccgcc
     1021 gcgcttccga ccttgtcgcg ggagcggttg aaggcgctga tctcgagcgg ccgggtttcc
     1081 ggccccgcgg gcctggttcg cgatccggcc gccaaggcga tggccggagt ctaccaagtc
     1141 gtcgttccgg aaccggcgcc cgcccataac gaagcccagg acatcgcgct cgaaatcgtc
     1201 ttcgaggacg atcacctgct cgtcgtcgac aagccggcgg ggatggtcgt ccatccggcg
     1261 gcaggcaatt tcgacgggac gatggtgaac gccctcctcc accattgcgc cggacggctg
     1321 tcgggcatcg gaggcgtggc gcggccgggg atcgtccatc gcatcgacaa gggcacttcg
     1381 ggccttctcg tcgtcgccaa gacggaccgg gcccatgagg ggctggcggc gcagttcgcc
     1441 cggcacagca tcgaccggcg ctatctggcc ctggtcgccg ggatcccgag cccggccgcc
     1501 gggaccatcg acgcccccct cgcccgctct tcggccaacc gccagaagat ggcgatcgtc
     1561 gaaggaaatc ggggcaagcg ggcggtgacc cgttatcgcc tgttgcggcc gctctgcgac
     1621 tcggctttgg tcgaatgccg gctcgaaacc gggcggacgc atcaggtcag ggtccacatg
     1681 acctcgatcg gcaacccgct gctcggcgat ccggcttacg gacggacccg atcgtcccat
     1741 cgcgagttat tgaaaaggct cggtttcgag cggcaggcgc ttcatgccgc ggagctcggg
     1801 ttcatccatc ccgtgact
//
LOCUS       DASWNU010000663         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_51602, whole genome shotgun sequence.
ACCESSION   DASWNU010000663 DASWNU010000000
VERSION     DASWNU010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51602"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VGB57_13325"
     CDS             complement(<1..318)
                     /locus_tag="VGB57_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074555.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0132375.1"
                     /translation="MAERWTDDRNNVLLLGAVLLALAIVVGGYLLGDGLRRARMADRA
                     VTVRGLAERNVTADLASWTIAYSEQGTELGPVQAAVDEKSRAVRAFFRRHGFADKDLS
                     DTGA"
     gene            737..1153
                     /locus_tag="VGB57_13330"
     CDS             737..1153
                     /locus_tag="VGB57_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0132376.1"
                     /translation="MKYLHTMIRVSDPDSTLRFFELLGLKELRRFDNEAGRFTLIFLA
                     APGDEDAQVELTHNWDEKGYGEGRNFGHLAYRVDNIYESCRRLMEGGVTINRPPRDGR
                     MAFVRSPDNISIELLQAGEALPPAEPWASLANTGHW"
     gene            1158..1565
                     /locus_tag="VGB57_13335"
     CDS             1158..1565
                     /locus_tag="VGB57_13335"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0132377.1"
                     /translation="MADLLLNIDVPDMERGIEFYTAALGLEVGRRFDPGFVELVGRDV
                     PIYLLCKDAGTPIGPAGGDRRRYERHWSPVHVDFVVDEIDSAVARATAAGAVREGETC
                     DAPYGKLAMFADPFGHGFCLIQFNSEGYDAIVA"
     gene            1549..2286
                     /gene="gloB"
                     /locus_tag="VGB57_13340"
     CDS             1549..2286
                     /gene="gloB"
                     /locus_tag="VGB57_13340"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY0132378.1"
                     /translation="MPSSLEIVRIPVLSDNYVWLVHEPGSNETAVVDPALAGPVLAEA
                     EARGWRITQIWNTHWHPDHTGGNSAIKAATGAFVTGPAAEAARIPTLDRQVVEGDRVT
                     LGGIEAEVLEVPAHTAGHIAYHLPSQEAVFVGDTLFAMGCGRLFEGTAEQMHSNLQRL
                     AMLPPETRAYCAHEYTLSNGRFAVTVEPGNEALALRMVEVEAARAAGEATVPTTIALE
                     LATNPFMRASSAAELAERRRAKDNFRA"
     gene            2334..2747
                     /locus_tag="VGB57_13345"
     CDS             2334..2747
                     /locus_tag="VGB57_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132379.1"
                     /translation="MRYLLLAASACLAAGCTTQTDEQRVDMAANDEASLAAQLRGFEP
                     AGPPVQCVSSRDLRGNRSAGETAIVFSGTGGRLWVNRPAAGCPDLRMSRALRTRTPST
                     RLCRGDIATVFDPLTRFEYGGCGLGEFEPYRRVRG"
     gene            complement(2918..>3286)
                     /locus_tag="VGB57_13350"
     CDS             complement(2918..>3286)
                     /locus_tag="VGB57_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0132380.1"
                     /translation="PQKVEGMLTLQPEILQAMVVGDRRPYLVGLLVPDPEWTAEWAQA
                     NGKAKGDGLRDDPDYHRALSAAVDRVNADLSVIEKVRRFILADAPFSTENEQLTPSIK
                     IRRHVIRAAYGERLDGLYRK"
BASE COUNT          515 a         1170 c         1048 g          553 t
ORIGIN      
        1 ggcgccggtg tcgctgaggt ccttgtcggc gaatccgtgg cgccggaaga aggcgcgcac
       61 cgcccggctc ttctcgtcga ccgccgcctg gaccggcccc aattccgtgc cctgctccga
      121 ataagcgatc gtccagctgg cgagatcggc cgtgacgttc ctctccgcga gcccgcgcac
      181 cgtcaccgcc cggtcggcca tccgcgcccg cctcagccca tcgccaagca ggtagccgcc
      241 gacgacgatc gccagcgcca gaagaaccgc cccgaggaga agcacgttgt tgcgatcgtc
      301 ggtccagcgc tcagccatgc catcctcctc cggccacacc gcgctttgtt acgctgccga
      361 tgaaccgtgc atttatgacg atgaatcgag cccaggcaag ccgtttcgaa tgcaggcgat
      421 gggcgagcct gcacgattcg ccgggaaaag ccccacttgt atctttgttc tatatgtgtt
      481 ctgctgatgg ccatgcgctc gccattcccc gatttgcgtc tgcccggctg ccggttcggg
      541 cctgccgggg ctgtcgcaac cgggacgggc ttggccggat tcgcaagttc caggcgccgc
      601 gggaaggatt tttttccctc cttggaagcc gaaactcaga aacttatgaa acttccggcc
      661 cggtcccgaa ctttccgccg ctgtgcttca cgcacccgcg accccctgcc ataggaggcc
      721 cgaaacggag cccgcgatga aatatctcca caccatgatc cgggtctcgg acccggactc
      781 caccctccgc ttcttcgagc tgctcggcct gaaggagctg cgccgcttcg acaacgaggc
      841 cggccgcttc accctcatct tcctcgccgc gcccggcgac gaggacgccc aggtcgagct
      901 cacccacaat tgggacgaga aaggctatgg cgaggggcgc aatttcggcc atttggccta
      961 tcgggtcgac aatatctacg agagctgccg ccggctgatg gaaggcgggg tgaccatcaa
     1021 ccgcccgccg cgcgacggcc gcatggcctt cgtccggagc ccggacaaca tctcgatcga
     1081 attgctccag gccggcgagg cgctgccgcc agccgagccc tgggcctcgc tggccaacac
     1141 cggccactgg tagggccgtg gccgacctgc tcctcaacat cgacgtgccc gacatggagc
     1201 gggggataga attctacacc gccgcgcttg ggctcgaggt aggccggcgc ttcgatccgg
     1261 gctttgtcga gctggtcggc cgcgacgtgc ccatctacct gctgtgcaag gacgccggca
     1321 ctccgatcgg cccggcgggc ggcgaccgcc gccgctacga gcgccactgg agcccggtcc
     1381 atgtcgattt cgtcgtcgac gaaatcgatt cggccgtcgc ccgcgccacc gccgccggag
     1441 cggtccggga aggcgagacc tgcgacgcgc cttatggcaa gctcgccatg ttcgccgatc
     1501 cgttcggcca cggcttctgc ctgatccagt tcaattcgga gggttacgat gccatcgtcg
     1561 cttgagatcg tccggatccc cgtcctctcc gacaattacg tctggctggt ccacgaaccg
     1621 ggatcgaacg agaccgccgt cgtcgatccc gcgttggccg ggccggtgct cgcggaggcg
     1681 gaggcgcgcg gctggcggat cacccagatc tggaacaccc actggcatcc cgaccacacc
     1741 ggcggcaatt cggcgatcaa ggccgccacc ggggcgttcg tcaccggccc ggcggccgag
     1801 gcggcgcgga tcccgacgct cgatcgccag gtcgtcgagg gcgaccgggt gacgctcggc
     1861 gggatcgagg cggaggtgct cgaggttccg gcccacaccg ccggccacat cgcttatcat
     1921 ctgccgtcgc aggaagcggt cttcgtcggc gataccctgt tcgcgatggg ctgcgggcgc
     1981 ctgttcgaag gcacggccga gcagatgcac tccaacctcc agcgcctggc gatgctcccg
     2041 cccgaaaccc gcgcttattg cgcgcacgaa tacaccctct ccaacggccg cttcgcggtg
     2101 acggtcgagc ccggcaacga agcgctcgcc ttgcggatgg tggaggtcga ggcggctcgc
     2161 gccgcgggcg aggcgacggt gccgacgacg atcgctctgg agcttgccac caacccgttc
     2221 atgcgcgctt catcggcggc ggagcttgct gagcggcgcc gggccaagga taatttccgc
     2281 gcatagggga ccgctcggca aatcgggcgt tacagccccg aggagtgccc aggatgagat
     2341 atctgctact tgccgcttca gcctgcctcg ccgccggctg caccacccaa accgatgaac
     2401 aacgggtcga catggcggcc aatgacgagg cctcgcttgc ggcccagctg cgcggcttcg
     2461 agcccgccgg accgccggtc cagtgcgtca gctcgcgcga cctcaggggc aatcgctccg
     2521 ccggcgaaac cgcgatcgtc ttctccggca ccggcggcag gttgtgggtc aaccgccccg
     2581 ccgccggctg ccccgatctg cgcatgagcc gcgccttgag gacccgtacc cccagcaccc
     2641 gcctgtgccg cggcgacatc gccaccgtgt tcgatccgct cacccgcttc gaatatggcg
     2701 gctgcggcct cggcgagttc gagccgtatc ggcgggtcag gggctagatc ttgcctcccc
     2761 cattgcgtcg caatggggag ggggatcgcc cgagggtggt ggaggggtga caaggctcgc
     2821 ctgcgaggtt cgagctcgat ccctcggcgt ccctgaccgg tcacccctcc accatgcttc
     2881 gcatggtccc cctccccatc tgcgatgggg aggccaatca cttcctgtag agcccgtcca
     2941 gccgctcgcc ataagcggcc cggatgacgt ggcggcggat cttgatcgag ggggtgagct
     3001 gctcgttctc ggtcgagaag ggggcgtcgg cgaggatgaa gcgccggacc ttctcgatca
     3061 ccgacagatc ggcattgacc cggtcgaccg ccgccgacag cgcccggtga tagtcgggat
     3121 cgtcgcgcag cccgtctcct ttggccttcc cgttggcctg agcccattcc gcggtccatt
     3181 ccggatcggg gaccagcagt ccgacgagat agggccggcg gtcgccgacc accatcgcct
     3241 gcaatatctc cggctggagg gtcagcatcc cctccacctt ctgcgg
//
LOCUS       DASWNU010000664         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_388265, whole genome shotgun sequence.
ACCESSION   DASWNU010000664 DASWNU010000000
VERSION     DASWNU010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388265"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..508)
                     /locus_tag="VGB57_13355"
     CDS             complement(53..508)
                     /locus_tag="VGB57_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0132381.1"
                     /translation="MITAIAGLMSSGPVQADELAAGDRLESLTAVVGTLDETLRAIDP
                     VVAEEKVSEAIGQGTASYYGSELAGNRTASGERFNPHDLTAAHRTLPLGTRLRVTNVA
                     NGRSVIVRVNDRGPFVGKRLIDVSLGAAREIQMIRSGTAQVRLEIVPTV"
     gene            707..>2283
                     /locus_tag="VGB57_13360"
     CDS             707..>2283
                     /locus_tag="VGB57_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HEY0132382.1"
                     /translation="MREKELRLALICYGGISLAVYMHGITKEVWRLARASQAFHSGED
                     AEPGSEAVYRRLIEAIARECELDVRVLVDILAGASAGGINAVFLAEAIASGRSLEPLT
                     DLWLETADVDRLIDPAMSRFSRLAKSAAVPLAWAWSSRRGALEKTVEAEHREEIRGKL
                     ANFVRARWFAPPFSGAGFTRLLLGAFDAMATGGQGAPLLPSYHPLDLFVTVTDFYGYS
                     EQLRLNSPPEVIEQEHRLILSFRRAGPGPLADAAELTFAARATASFPGAFPPFQVAEL
                     DSVLKERGTDWPGRSDFLTRALPRQSALGTAETAVLIDGSVLANAPFRPAIKALQNRP
                     AHREVDRRFVYIDPKPGVRSVRLAAGGGDEAPGFFATIFGALSDIPREQPIRDNLEAI
                     DQRSSTIRRLRRIVEAMRPEVEAAIERAFGSTIFLLKPTAKRLAGWRARAQNVAAREA
                     GYAYAAYGHFKLAGVAEDAAERIFQLGGGGDRSRHERIRQAVWSEVRRLGLADQDSMT
                     KAGARPEVVLFLRSFDAA"
BASE COUNT          334 a          805 c          776 g          368 t
ORIGIN      
        1 ctgaacttgt ttcagggtcc agtcgatcat gcagcggctt tcgccgcgtg gctcaaaccg
       61 tcgggacgat ctccaggcgg acctgggcgg ttccggagcg gatcatctgg atctcgcggg
      121 cggcgcccag ggagacgtcg atcaggcgct tgccgacgaa cggcccgcga tcgttgaccc
      181 ggacgatgac gctccggccg ttggcgacgt tggtcacccg aagcctggtt cccagcggca
      241 gcgtgcggtg ggcggcggtg aggtcgtggg gattgaagcg ctcgccgctc gcggttcggt
      301 tgccggcgag ctcgctgccg taatagctgg cggtgccctg gccgatcgcc tcggagacct
      361 tttcctcggc gacgacggga tcgatggcgc ggagggtttc gtcgagcgtc ccgaccaccg
      421 ccgtcaggct ttcgaggcgg tcgccggcgg cgagctcgtc ggcctggacc gggcccgagc
      481 tcatcagccc ggcgatggca gtgatcacgg caaattgagt gccgcggcga cgcaatcgcg
      541 aaaacaacat aagctttgtg gcccctcagc ctccgatggg acccgggcta accggctaat
      601 ctaaatgagt cccggtcgtt acggcggcgt gaaacgattc cgggatgagt tgagccgagt
      661 tccgtccgtc tcgacaactc gtcgctcgcc tgccacgatt catcctgtgc gcgaaaagga
      721 attacggctg gccctgatct gctacggcgg aatcagcctc gccgtttaca tgcacggcat
      781 caccaaggaa gtctggcgcc tcgcccgcgc cagccaagcc ttccactccg gcgaggacgc
      841 ggagccgggc agcgaggccg tctaccgccg cctgatcgag gcgatcgccc gcgaatgcga
      901 gctcgacgtc cgggtcctgg tcgacatcct cgccggggcc agcgcgggcg ggatcaacgc
      961 cgtcttccta gccgaggcga tcgcaagcgg ccgctcgctc gagcccctga ccgacctgtg
     1021 gctcgaaacc gcggatgtcg accggctgat cgaccccgcc atgagccgct tctcccgcct
     1081 cgccaaatcg gcggcggtgc cgctcgcctg ggcctggtcc tcgcggcggg gcgccctcga
     1141 gaagacggtg gaggccgagc atcgcgagga gattcgcggc aagctcgcca atttcgtccg
     1201 cgcccgctgg ttcgccccgc cgttcagcgg ggccggcttc acccgcctgc tgctcggcgc
     1261 gttcgacgcc atggcgaccg gcgggcaagg ggccccgctc cttccgagct accatccgct
     1321 cgacctgttc gtcaccgtca ccgacttcta cggctattcc gagcagcttc ggctcaactc
     1381 gccgccggag gtgatcgagc aggagcaccg gctgatcctc tccttccgcc gggcggggcc
     1441 gggcccactc gccgatgccg ccgagctcac cttcgcggct cgggcgacgg cgagcttccc
     1501 cggcgccttc ccacccttcc aggtggccga gctcgactcg gtgctgaagg agcgcggcac
     1561 cgactggccg ggccgaagcg acttcctcac cagggccttg ccgcgccagt cggcgctcgg
     1621 aacggctgaa acggcggttc tgatcgatgg ctcggttctc gccaacgccc cctttcgccc
     1681 ggcgatcaag gcgctccaga accggccggc gcaccgcgag gtcgaccgcc gcttcgtcta
     1741 tatcgatccc aagccgggcg ttcgttcggt gcggctggcg gcgggcgggg gcgacgaggc
     1801 gcccggcttc ttcgcgacca tcttcggagc gctttcggac attccgcgcg agcagccgat
     1861 ccgcgacaat ctcgaagcga tcgaccagcg ctcgtcgacc atccgccggc tgcgccggat
     1921 cgtcgaggcg atgcgacccg aggtcgaggc ggcgatcgag agggccttcg gctccaccat
     1981 cttccttttg aagccgacgg cgaagcgcct cgccggctgg cgcgcccggg cccagaacgt
     2041 cgccgcccgc gaggccggct atgcctatgc cgcctacggc catttcaagc tcgccggagt
     2101 ggcggaggac gcggcggagc ggattttcca gctcggcggc ggcggcgacc gaagccgcca
     2161 cgaacggatc cgccaggcgg tgtggagcga agtgaggcgg ctcggcctcg ccgaccagga
     2221 ttcgatgacc aaggccggcg cccggcccga ggtcgtgctc ttcctgagga gcttcgacgc
     2281 agc
//
LOCUS       DASWNU010000665         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_466572, whole genome shotgun sequence.
ACCESSION   DASWNU010000665 DASWNU010000000
VERSION     DASWNU010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466572"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VGB57_13365"
     CDS             <1..1439
                     /locus_tag="VGB57_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132383.1"
                     /translation="ILIANLEAMPTPVGARPAAAKPYKWFTNAAPGGTPPLQPVSHAA
                     NHRIRCAFDPVTGRLIAAPPVGTAKEVADVRIAYGYGIGREIGAGPQDRNDEDVPFDI
                     IDTADLQHFVRVVDAEASATAPANVVKTLAAALVDWKALGAGKRGLIVLVRCDLDSSG
                     SLAVAVHPASELHIVSAQWRPRTGPRHGYVVRKDRIFTIGGQLKVEPQPVGAKGGALI
                     LDGLEITGGVLLGARAVSSLTVRYCTLRAPGAAALDTTIALNDTDIVIHRSIVGRIRL
                     DVGAAVGTGSLAVAGSLVSADGFAGAAITAGTLDTRLADVTVFGPCAFKSLEATNVIF
                     DATAAVAVTRTQVGCVRFSWVPPGSKTPRRFRCQPDLAVQTEAARKGAPLSAAERSAT
                     TLSVTPIFLDTALGEPTAAMLHPLTSAAIASGGEGDSEMGVFAASAERLRMSNIESLF
                     DDYVPFGLEAGLIDDTRSSAVAQRRNRP"
     gene            1436..4081
                     /locus_tag="VGB57_13370"
     CDS             1436..4081
                     /locus_tag="VGB57_13370"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEY0132384.1"
                     /translation="MTNRFDGTRMRVPVKERRAFRGVDVSQGMVLLDVDLNEQGAALL
                     NRIEDETVDILGSKGKFVYPAGDTGFRITGSAPAANFDIGAGHGYLDGWLLENPAVCK
                     LGTQPHPRTGDVVNPVAIIALKALVRHVDRVEEPALADVALGDAQASGRAVNDWQVFP
                     LAMPVPAGGSLSCAEAALRVEWAHVIAPSTGTMTVIKQAAGPSSDPCSLTPGGGYTRL
                     ENRLYRVEVHGGVPKPGVLQADGPRYSGVGLIVKVQSRNASLMVRIVDIVGSEIKVEP
                     AALDARHWFAPGAYAEIVNVHDDVDPRAALANERLFKVALATDDRIVLAAPAAAIAAT
                     LAAKDGNWFLRLWDAFPSGDGTAVVTGGASQVIDLGDGLSIRFQGGIGATYRRGDYWT
                     FAARADGSIDWPETVPGTPVPMVPHGPETRYAVLAVAGGTAAAPVFDNCSIPFASLTD
                     RLLFYRGGDGQSGFIPPPGPAMSPFVLLPQRLRVAVMRGERPVAGAVIRWTPPPAAPD
                     SRVAGQPCTPGSAVVSITDANGLAEVDWEIDPARPLDLHRVQATLVTSPTTNLANTML
                     FHAQFATAARTSYTPGRCKHLLNVFNVQEAIDALCQKVETRPPVMTLTEIKLLQPNGQ
                     PIELIRENLILNGLEIRNDAFVNGISFGFDLGMPTIAITPWDPVVEVELDLPYPSTDP
                     DRAYWGLASRIAVPNAAPRQVRGPVGFQRLRLDGTVRLAPAQAGRQAGLLWQPSPEAQ
                     QFLQTATEHRFGQKFNQSSLVSLRDAGFLAESAFRRILCRVHVRSAMIWVGNGANRAY
                     LNAEHLGTRGPFTGRELDLRARDPQSAADLDFFLYLTPPNFSTDVTFPLDVNVLGNLH
                     GIATRDLNLLTRDVALTDGVVAPNS"
     gene            complement(4140..4607)
                     /locus_tag="VGB57_13375"
     CDS             complement(4140..4607)
                     /locus_tag="VGB57_13375"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0132385.1"
                     /translation="MRPSGLSGIAITNGPAVSPDGRILYWVDTVGQTINACRIADDGT
                     LGESRLLVKLGAKEGFPDGPTVDSEGCVWISLYWGWEARRYSPEGELIERLSFPVSNI
                     TKIAFGGDDFRTAYATTARQGIEAEAMAGQPEAGSVFAFEVDVPGMESPKAQL"
     gene            4582..>4742
                     /locus_tag="VGB57_13380"
     CDS             4582..>4742
                     /locus_tag="VGB57_13380"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0132386.1"
                     /translation="MPLRPDGRILYWVDTLGGTVEACDVADDGTLGPSRPFVTIDPAD
                     GTPDGPSVD"
BASE COUNT          713 a         1735 c         1571 g          723 t
ORIGIN      
        1 agatcctgat cgccaatctg gaggcgatgc cgaccccggt cggcgcccgg ccggccgcgg
       61 ccaagcccta taaatggttc accaatgccg ctcccggcgg gacgccgccg ctgcagccgg
      121 tctcccacgc ggccaaccac cgcatccgct gcgccttcga cccggtgacg ggccggctga
      181 tcgcggcgcc gccggtagga acggcgaagg aggtggccga cgtccggatc gcctatggat
      241 acgggatcgg cagggagatc ggcgccggtc cgcaggaccg caacgacgag gacgtaccgt
      301 tcgacatcat cgacacggcc gatcttcagc atttcgtccg cgtcgtcgat gccgaggcgt
      361 cggccaccgc gcccgcgaac gtcgtcaaga cccttgccgc ggcgcttgtc gactggaagg
      421 ctttgggagc cggcaagcgc ggactgatcg tcctcgtccg ctgcgacctc gacagctcgg
      481 gaagcctggc ggtcgccgtc catccggcct ccgagctgca catcgtcagc gcccagtggc
      541 ggccgaggac cggcccgcgt cacggctacg tggtccgcaa ggatcgcatt ttcaccatcg
      601 gcggccagct caaggtcgaa ccgcagccgg tgggcgccaa aggcggggct ctgatactcg
      661 acggattgga gatcacgggc ggcgtcctgc tcggcgcccg cgcggtttcg tcgctcacgg
      721 tccgctactg cacgctgcgc gcgccggggg ccgcggcgct cgacacgacc attgccctca
      781 acgacaccga catcgtcatc caccgctcga tcgtcggacg catccggctg gacgtcggcg
      841 ccgccgtcgg gaccgggtcg ctcgccgtcg ccggatcgct cgtctccgcc gacggcttcg
      901 ccggagccgc gatcacggcc ggcacgctcg atacgcgcct tgccgacgtg accgtcttcg
      961 gcccctgcgc gttcaagtcg ctggaagcga ccaacgtcat cttcgacgcg accgcggcgg
     1021 tcgcggtcac ccggacccag gtcggctgcg tccgcttctc ctgggtcccg ccggggtcga
     1081 agactccgcg acgcttccgc tgccagccgg atctcgccgt ccagacggag gcggcacgca
     1141 agggcgcgcc gctgagcgcg gccgagcgga gcgccacgac gctctcggtg acgccgatct
     1201 tcctcgacac cgcgctcggc gagccgaccg ccgcgatgct ccatccgctg accagcgccg
     1261 ccatcgccag cggcggcgaa ggcgacagcg agatgggagt cttcgccgcg tcggcggagc
     1321 gcctcaggat gagcaacatc gagagcctgt tcgacgatta cgtgccgttc ggcctggaag
     1381 cgggcctcat cgacgacacc cgctccagcg ccgtagccca gcgaaggaat cgaccatgac
     1441 caatcgtttc gacggcacca ggatgcgtgt cccggtcaag gagcgccgcg catttcgcgg
     1501 cgtggacgtg agccagggca tggtcctcct cgacgtggat ctgaacgagc agggcgctgc
     1561 cctgctgaac cggatcgagg acgagaccgt cgacatcctc gggtcgaagg gcaagttcgt
     1621 ctatcccgcc ggcgataccg gcttccggat cacgggcagc gctccggccg ccaatttcga
     1681 catcggcgcc ggtcacggct atctcgacgg ctggctgctg gagaatccgg cggtctgcaa
     1741 gctcggcacc cagccccacc cgcgcaccgg cgacgtcgtc aacccggtcg cgatcatcgc
     1801 cctcaaggcg ctggttcggc acgtcgatcg ggtcgaggag ccggcgctgg ccgacgtcgc
     1861 gctcggcgac gcccaggcgt cgggccgggc ggtcaacgac tggcaggtct ttccgcttgc
     1921 catgccggtt cccgccggcg gctcgctgag ctgcgcggag gccgccctca gggtggagtg
     1981 ggcccacgtc atcgcccctt cgaccggcac gatgacggtg atcaagcagg cggcgggccc
     2041 gtcctccgac ccctgctcgc tcactccggg cggcggctat acccggctcg agaaccggct
     2101 ctaccgcgtg gaggtccatg gcggcgtgcc caagccgggc gtgttgcagg ccgacgggcc
     2161 gcgttattcc ggagtcggac tgatcgtgaa ggtgcagagc cggaatgcca gcctgatggt
     2221 ccgcatcgtc gatatcgtcg gcagcgagat caaggtcgag ccggcggcgc tcgatgcccg
     2281 gcactggttc gcgccgggag cctatgccga gatcgtcaac gtccacgacg acgtcgatcc
     2341 gcgcgccgcg cttgcgaacg aaaggctgtt caaggtggcc ctcgccaccg acgaccgcat
     2401 cgtgctcgcc gcacccgccg ccgcgatcgc cgccactctc gccgcgaagg acggcaactg
     2461 gttcctgcgg ctctgggatg cgttcccgag cggcgacggc accgcggtcg tgaccggcgg
     2521 cgcgtcgcag gtcatcgatc tcggcgacgg cctctcgatc cggttccagg gcggtatcgg
     2581 cgccacttac cgacgcggcg attactggac cttcgcggcc cgcgccgacg gctcgatcga
     2641 ttggccggaa accgtccccg gcacgcccgt gccgatggtc ccgcacggcc ccgagacgcg
     2701 ctatgcggtc ctcgcggtcg ccgggggcac ggccgccgcg ccggtgttcg acaattgcag
     2761 catcccgttc gccagcctca ccgaccgcct gctcttctat cgcggcggcg acgggcagag
     2821 cggtttcatc cctccgcccg gcccggcaat gtcgccgttc gtcctgctgc cgcaaaggct
     2881 gagggtggcg gtgatgcgcg gcgagaggcc ggttgccggt gccgtcatcc gctggactcc
     2941 gccgccggcg gcgcccgatt cgcgcgtcgc gggccagccc tgcaccccgg gctcggcggt
     3001 ggtttcgatc accgacgcca acgggctcgc cgaggtcgac tgggagatcg acccggccag
     3061 gccgctcgac ctccaccgcg tccaggcgac gctggtcacg tcgccaacca cgaacctcgc
     3121 caacaccatg ctgttccacg cccagttcgc caccgcggcg cggaccagtt atacaccggg
     3181 caggtgcaag catctgctca acgtcttcaa tgtccaggag gcgatcgatg ccttgtgcca
     3241 gaaggtcgag acccgccccc ccgttatgac cctgacggag atcaagctgc tccagccgaa
     3301 cgggcagccg atcgaactga tccgcgaaaa cttgatcctc aatgggttgg aaatccgcaa
     3361 cgacgcgttc gtcaacggca tcagcttcgg cttcgacctc ggaatgccga cgatcgcgat
     3421 cacgccctgg gatcctgtcg tcgaggtcga gctggacctc ccctacccga gcaccgatcc
     3481 cgaccgggcc tattggggcc tggcgtcgag gatcgccgtg cctaacgccg cgccccgaca
     3541 ggtgcggggg ccggtcggtt tccagcgcct tcgcctcgac gggacggtga ggctcgcccc
     3601 ggctcaggcg gggcggcagg cgggactgct ctggcagccc tcgccggagg cccagcaatt
     3661 cctccagacg gcgaccgagc accgcttcgg acagaagttc aaccagtcca gcctcgtatc
     3721 cctgagggac gcaggcttcc tcgccgaatc ggccttccgc cgcatcctgt gccgggtcca
     3781 tgtccgctcg gcgatgatct gggtcggcaa cggcgccaac cgcgcctatc tgaatgccga
     3841 gcatctcggc acgcgcggcc cctttacggg gcgggagctg gacctgcgcg cgcgagaccc
     3901 gcaatcggcg gccgacctcg atttcttcct ctatctgacg ccgccgaact tcagcaccga
     3961 cgtcaccttc ccgctcgatg tgaacgtatt gggcaatctt cacgggatcg ccacccgcga
     4021 cctgaacctg ctcacgcgcg acgtagccct gaccgacggg gtggttgcgc cgaactcgta
     4081 gatccgcaca gcgggccgta cgagccgcaa ggggcgccgg gacccggggc cagctccgct
     4141 cagagctgcg ccttcggact ctccatgccg ggcacatcca cctcgaacgc gaacaccgag
     4201 cccgcctcgg gctgcccggc catcgcctcc gcctcgatgc cctggcgcgc ggtcgtcgca
     4261 taggccgtcc ggaagtcgtc gccgccgaag gcgatcttgg tgatgttgga gacggggaag
     4321 gataggcgct cgatcagctc gccttccggg gaatagcgcc gcgcctccca gccccaataa
     4381 aggctgatcc agacgcagcc ttcgctgtcc accgttggcc cgtccgggaa gccttccttg
     4441 gccccgagct tcaccagcag ccgcgactcg ccgagcgtgc cgtcgtcggc gatgcggcag
     4501 gcgttgattg tctgcccgac cgtgtcgacc cagtagagga tgcggccgtc gggcgacacg
     4561 gcggggccgt tggtgatggc gatgccgctg aggccggacg ggcgcatcct ctactgggtc
     4621 gacaccctcg gcggtaccgt cgaggcgtgc gacgtcgcgg acgacgggac gctcggcccc
     4681 tcgcgcccgt tcgtcaccat cgatcccgcc gacggcacgc ccgacggccc cagcgtggac
     4741 ag
//
LOCUS       DASWNU010000666         8343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_335997, whole genome shotgun sequence.
ACCESSION   DASWNU010000666 DASWNU010000000
VERSION     DASWNU010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335997"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..3156)
                     /gene="addB"
                     /locus_tag="VGB57_13385"
     CDS             complement(208..3156)
                     /gene="addB"
                     /locus_tag="VGB57_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEY0132387.1"
                     /translation="MTSVRPSVFTVPPHRAFADALAAGLIARYGRGDLGLAQGIVLVP
                     NNRAARAVADAFVRRSGGGLLLPRLVPVGDPELDERIGGALEPLGEGEGLPPAVEPLE
                     RLFLLARLIQQHVGGVDAAEALRLAEDLARTLDQLLVEGVEPSNLRDFAAELPDLSLH
                     WQASLERLGIILDQWPRLLSERGRIDLADRRNRLLAMVAARWRTSPPPGFVAAAGITS
                     AAPAIAGLLRTVSRLDDGLVVFPGLDLQMGPDEWDSLGPHEPDETTGFRKRSIETHPQ
                     FHLKLLLDRMGVGRGEVEGWRWGGGRDAPAARSRAIANAMKPAAFTDRWQVLRPAERR
                     LTGVRALELADPAEEAQTIAVALREALETPGRTAALVTPDRNLARRVSTHLRRWGVEA
                     DDSAGRPLSQLAPGTLLLALAAAAAEQFAPVSLLALLKHPLVMKGEGRLDWLDGARAL
                     DIALRGPRPPPGLDGITGHLSDRSGRDYKKRERASSWWSGVAPRLRALERGFAVERAA
                     LPSLLGLLRESASELSGDEAWAGPAGRAAAELLESAEPASAHGPSRLSPASLPALLEQ
                     LLAAVAVRPPYGQHPRLFIWGLIEARLQSADLVILGGLNEGVWPQLPTPDPWLAPRVR
                     RELGLPSLERRIGLAAHDFASALGGREVLVTRARRDARSPAIASRFWLRLEAMTAGVT
                     RSPRHRSWARALDRPTDFLPAARPAPSPAAELRPRRSAVTDVDRLKADPFAFYARKML
                     DFAPLDPIDADPSAAWRGSAVHKVLEAWMKEDDCEPSRLRPRAEALLGDSSAHPLMRA
                     LWQPRLLEAIDWIAAEMSKNLALGRRPVAAETKGTCEVEGIALYGEADRIDRFADGTL
                     AIVDYKTGTPPGPKAVAEGYAMQLGLLGLIAERKGFEGIEGVPACFEYWSLARKAGRL
                     GYVASPVTSRGGIDVAEFTTMAARNFLGAAARWLTGDEPFTAKLHPEHAPYGDYDQLM
                     RLDEWYGREV"
     gene            complement(3410..4174)
                     /locus_tag="VGB57_13390"
     CDS             complement(3410..4174)
                     /locus_tag="VGB57_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0132388.1"
                     /translation="MNRQRRPLALRPVPAGDVPKTAMVLAAGLGKRMRPLTATRPKPL
                     VEVAGKPLLDHCLDRLRTAGVEKVVVNVHYLADALEAHLKNRVQDLEVSVSDERQQLL
                     ETGGGMVRALPLIDADPFLVVNSDNLWIDGPVDSLRLLASNWDDARMDALLLLVPLAR
                     AHCHKGQGDFHMSSGGVLKRRKPGGVAPFVYTGVQIVSKRLLEGEIPEGPFSTTLLWD
                     RAIAGGRLFGAVHEGLWFDVGAPANIKQAEEVLGLA"
     gene            complement(4171..5175)
                     /locus_tag="VGB57_13395"
     CDS             complement(4171..5175)
                     /locus_tag="VGB57_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0132389.1"
                     /translation="MAAPMIPPAGAPDFLAEHGWDGAEIRPLAGDASFRRYFRVHRDS
                     QSAVLMDAPPEHEDIGPFLSVAGHLLDRGFCPPRPIAVDRNRGLLLLEDFGDDRVGPV
                     LQREPHREREIYGSAVDILARLAAQPAPAELAPYDDQAMEREVRLFPEWYVPALELQV
                     DEAGFLSAWRQVWGDVLRVVRENPVLVLRDYHADNLMVLPGRSELGLLDFQDALAGHA
                     AYDLVSLLQDARRDVSPELEEAMIDLYCERANVADRDLFRAHYEILGAQRNTKILGIF
                     TRLWKRDEKPFYPTMHPRIWGYVERNLAHPALGPVRAWFDSNVPSEFRNDACLRKIYA
                     "
     gene            complement(5160..5597)
                     /gene="tsaE"
                     /locus_tag="VGB57_13400"
     CDS             complement(5160..5597)
                     /gene="tsaE"
                     /locus_tag="VGB57_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY0132390.1"
                     /translation="MVLDGPEATEAIGRRLAAHLRAGDVVALFGELGAGKTTLARGIL
                     AGLGHDGEVASPTFPIVIPYDCVRIPVWHVDLYRLDGPAEIEELALDEALQDGALIVE
                     WPERMGRALWPHSLRLTLRRQGEGARALTAAVPAAWGARWPPR"
     gene            complement(5597..7942)
                     /locus_tag="VGB57_13405"
     CDS             complement(5597..7942)
                     /locus_tag="VGB57_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0132391.1"
                     /translation="MVNLSPTALAAIALLAGAWLAIALWATVRGLGRGRDATARIDAT
                     RHHEALLAASPALPLIVRRDGSLEEAERVGLALGLAATPRRLDDLGDTLAEEDRDSLL
                     GQVAATAATAAGLALSIRPAGSTRVYRVRGGPAPPSYPAGTVLLWLTDATESEEEMAA
                     LRERSERLASALDALSGLIEAAPFPIWHRGPDLRLAMVNSAYVAAVEAEDGRAVIEGG
                     LELVDDGEAGAIAEAAAVRQSGRPHARNVPATVGGERRMMRVVEVPLGEAGVAGYAID
                     VEDREQARSELARFVHAQRDMLDRLSAGVAQFARDRGLNFSNQPFARLFALRPDFLAD
                     RPEFNRLLDAMRETGHLPEVRDYPEWKKEHDRWFTGGLAAEEEDWLLPGGRHLRVVAQ
                     PLPDGGLLLIFEDRTEQLRLASARDTLLRVRTATFDNLFEAVGVFASDGRLHLWNNRF
                     RLLWEFDEEQLAGNPRIDALTTHIGKKLRNSSHAALVRELVRSATVERKQRNGRVAFT
                     DGREFEFAAVPLPDGNALFTMLDVTASRTVEAALRERTQALEQADRLKTAFVSNMSYE
                     LRTPLTSIGGFAEMLAGGYAGDLGPTASEYVTAILDSVARLGALIDDVLDLTQSDSGS
                     LLLAEDKVDLARLCAEAARAFKEQAAKKQIDFAVEIEAGAGSIAGDRARLRQSLDNVL
                     KNAVAYTGEGGRVFLHAAGNRKEAAITVSDNGRGIAPADQAGVFDRFSRTAEPRGADG
                     AALGLGLPLAKQFIEAHGGTIELVSEPGEGTTVTLRLPRKR"
     gene            8124..>8343
                     /locus_tag="VGB57_13410"
     CDS             8124..>8343
                     /locus_tag="VGB57_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132392.1"
                     /translation="MALTFRSRALRSVAFIGLLGALAPQAYAQETQGTEGTGAGETDD
                     PSQLNSEAEIESGQDASAADQGDITITGS"
BASE COUNT         1325 a         2843 c         3021 g         1154 t
ORIGIN      
        1 gttcgggacc gctgcgtcca gggccgttca gggcacgcca gggccaccgg ccatgccagg
       61 gccagccatg gccgccggat gcagctggcg atgagggccg gcgagcgggg ctgagcaacc
      121 gtcgccccag cgaaggccgg ggccccggcg ggtgaagacc gcggcgcctt tcgcctcccg
      181 gggtcccagc ctgcgctggg acgacgatca aacctcgcgc ccgtaccatt cgtcgaggcg
      241 catcagctgg tcgtaatccc cgtaaggagc atgttcgggg tggagcttcg ccgtgaacgg
      301 ctcgtccccg gtcagccagc gggcggccgc gccgaggaaa ttgcgcgcgg ccatcgtcgt
      361 gaattcggcg acgtcgatcc cgccccggct cgtcaccggg cttgcgacat agccgagcct
      421 gccggccttg cgggctaagg accaatattc gaagcaggcc ggaaccccct cgattccctc
      481 gaaacctttg cgctcggcaa taagcccgag caggccaagc tgcatcgcat agccttccgc
      541 cacggccttc ggaccgggcg gagtcccggt cttgtagtcg acgatggcga gggtcccgtc
      601 ggcgaaccgg tcgatccggt cggcctcgcc gtagagcgcg attccttcca cctcgcacgt
      661 ccccttggtc tcggcggcca cggggcggcg gcccagggcg aggtttttcg acatctccgc
      721 cgcgatccag tcgatcgcct ccaggaggcg gggctgccaa agcgcccgca tcagcgggtg
      781 ggcggaggag tccccgagca gggcttccgc ccgcggccga agccgcgacg gctcgcaatc
      841 gtcctccttc atccaggctt cgagcacctt gtggaccgcg ctcccgcgcc aggcggcgct
      901 cggatcggcg tcgatcggat cgagcggggc gaagtcgagc atcttgcgcg catagaaagc
      961 gaagggatcg gccttgagcc ggtcgacgtc ggtgacggcg ctccgccgcg gccggagctc
     1021 ggccgccggg ctcggcgccg gccgggcggc cgggaggaag tccgtgggcc ggtccagcgc
     1081 tcgcgcccag ctccgatgcc ggggcgaccg ggtcactccg gcggtcatcg cctcaagcct
     1141 caaccagaaa cgcgaggcga tggcgggcga gcgggcgtcg cggcgggcgc gggtgacgag
     1201 aacttcgcgg ccgccgagcg cgctcgcgaa atcgtgggcg gcaaggccga tccggcgctc
     1261 gaggctcggc aggccgagct cgcggcggac ccggggcgca agccagggat ccggggtcgg
     1321 caattgcggc cagaccccct cgttgagacc gccgaggatg acgagatcgg ccgactggag
     1381 ccgggcttcg atgaggcccc agatgaagag gcgcggatgc tggccgtaag ggggccgaac
     1441 cgccacggcg gcgaggagct gctccagcag ggcgggaagg ctcgccggcg acagacggga
     1501 ggggccgtgc gccgaagcgg gctcggccga ctcgagcaat tcggcagcgg cgcggccggc
     1561 cggtcccgcc caggcttcgt cgccgctgag ctcggacgcc gattcccgaa gcaggccgag
     1621 cagcgaggga agcgccgcgc gttcgaccgc gaagccgcgc tccagagcgc gaagccgggg
     1681 cgcgacgccg ctccaccacg aggatgcgcg ctcacgcttt ttgtagtccc ggccgctgcg
     1741 atcggagaga tgcccggtga tgccgtccag cccgggcggc ggccgcggac cgcgcagcgc
     1801 gatgtcgagg gcgcgggcgc cgtcgagcca gtccagcctc ccctccccct tcatgaccag
     1861 cggatgcttg agcaaggcga ggagcgacac cggcgcgaac tgctccgccg cggccgcggc
     1921 gagcgcgagc aggagcgtgc cgggcgcgag ctgggagagc ggtcggccgg cgctgtcgtc
     1981 ggcctcgact ccccaccgcc gaaggtgggt cgagacgcgc cgcgcgaggt tgcggtcggg
     2041 agtcaccaaa gcggcggtcc ggccgggagt ctcgagcgcc tcgcgaagag cgacggcgat
     2101 ggtctgcgcc tcttcggcgg gatcggccag ctcgagcgcc cgcaccccgg tgagacgccg
     2161 ctcggcggga cgcaggacct gccaccggtc ggtgaaggcc gcgggcttca tcgcgttggc
     2221 gatggcgcgg cttcgggccg ccggagcgtc gcggccgccg ccccagcgcc agccttccac
     2281 ttcgccccgg ccgacgccca tccggtcgag gagcagcttc aagtggaatt gcggatgggt
     2341 ctcgatcgat cgcttccgga acccggtggt ctcgtccggc tcgtgggggc ccagcgagtc
     2401 ccattcgtcc ggccccattt gaaggtcgag gcccggaaag acgacgaggc cgtcgtcgag
     2461 gcgggagacg gttcgaagca ggcctgcaat ggcgggagcg gcgctggtga tcccggcggc
     2521 ggcgacgaag ccgggaggcg gagaggtccg ccagcgggct gcgaccatgg cgagaaggcg
     2581 gttgcgccgg tcggcgaggt cgatccggcc ccgctcggag agaaggcgcg gccattggtc
     2641 gaggatgatc ccgagccgct ccagcgacgc ctgccagtgg agcgacagat ccggaagctc
     2701 ggcggcgaag tcgcgcaggt tcgacggctc caccccttcc acgagcagct ggtcgagcgt
     2761 ccgggcgaga tcctcggcga ggcgcaaggc ctccgccgca tccaccccgc cgacatgctg
     2821 ctggatgagg cgcgccagaa ggaacagccg ctcgagcggc tcgacggcgg gcggcaggcc
     2881 ctccccttcc ccgagcggct cgagcgcccc gccgatccgc tcgtccagct cggggtcgcc
     2941 caccggcacc agccggggca ggagcaggcc tccgcccgag cgccggacga aggcgtcggc
     3001 cacggcacgg gccgcgcggt tgttgggaac gaggacgatg ccctgcgcaa gccccaggtc
     3061 gccgcggccg tagcgcgcga tcagacccgc cgcgagcgcg tcggcgaaag cgcggtgcgg
     3121 cgggacggta aagacgctgg gacggacgga ggtcacgagg tcatcgccgg gcaagcccgc
     3181 ggaaggcgac aacagcgaca atcaagttgc ggaagtttcg gcttcccatg cagcggaaaa
     3241 aagcggcggc ctggttggcg ctgttgtcgc tgttcggcga cgctccggct ggaagggaaa
     3301 tgaatgccca tatgcggaac ataacatgaa tcaccaggtt cggcaaagtc cccctccgtg
     3361 agacggaact cgtttcggca tccgtgaggt tgcgagctta gccgccactt caggccagcc
     3421 caagcacttc ctcggcctgt ttgatgttgg cgggagcccc gacgtcgaac cagaggccct
     3481 catggacggc gccgaagagg cggcccccgg cgatcgcccg gtcccacagc aaggtggtgg
     3541 agaagggccc ctccggaatc tcgccctcga gcaggcgttt cgagacgatc tggacgccgg
     3601 tatagacgaa cggcgccact ccgcccggct tgcggcgctt cagcactccg ccggagctca
     3661 tatggaaatc gccctgcccc ttgtggcaat gggcgcgggc gagcggcacg aggagcagca
     3721 gtgcgtccat ccgggcatcg tcccaattgc tcgcgagcag ccgcagcgag tcgaccgggc
     3781 cgtcgatcca gagattgtcg gaattgacga cgaggaaggg atcggcgtcg atcagcggca
     3841 gcgccctgac cattccgccg ccggtctcga gcaattgctg ccgctcgtcc gacacggaaa
     3901 cctcaaggtc ttgcacccgg ttcttcagat gcgcttcgag cgcatcggcg agataatgga
     3961 cattgaccac gaccttctcg acaccggccg ttcgcagccg gtcgaggcaa tggtcgagca
     4021 gaggcttgcc ggcgacctcg acgaggggct tggggcgggt ggcggtgagc ggccgcatgc
     4081 gcttgcccag gcccgcggcg aggaccatcg ccgtcttcgg cacgtcgccg gccggcacgg
     4141 gtcggagggc gagcggccgg cgctggcggt tcatgcgtag attttcctga ggcaggcgtc
     4201 gttgcgaaat tccgagggca cgttggagtc gaaccaggcg cggaccggac cgagggcggg
     4261 atgggcgaga ttgcgctcga catagcccca gatgcgggga tgcatggtcg ggtagaaagg
     4321 cttctcgtcg cgcttccaga ggcgggtgaa gatgccgagg atcttggtgt tccgctgcgc
     4381 tccgaggatc tcgtaatgcg cccggaagag gtcgcggtcg gcgacgttcg cacgctcgca
     4441 atagaggtcg atcatcgcct cctcgagctc gggcgacacg tcccgccggg catcctggag
     4501 aagggagacg agatcgtagg cggcgtggcc ggccagggcg tcctgaaagt cgaggaggcc
     4561 gagctccgac cggcccggaa gcaccatcag attgtcggca tgatagtcgc gaaggaccag
     4621 caccggattt tcccggacga cgcgcagaac gtcgccccag acctgccgcc aggcggagag
     4681 gaagccggcc tcgtcgacct gcagttcgag cgccggaacg taccattccg ggaagaggcg
     4741 gacctcccgc tccatcgcct ggtcgtcata gggcgcaagc tcggcaggag ccggctgcgc
     4801 cgccagccga gcaaggatgt cgacggcgct cccgtagatt tcccgctccc ggtgcggctc
     4861 gcgctggagg accgggccga cccggtcgtc gccgaaatcc tcgagcagga ggaggcccct
     4921 gttccggtcg acggcgatgg ggcgcggagg gcagaagccg cggtcgagca ggtggccggc
     4981 aaccgaaagg aacggcccga tatcctcatg ctccggcggc gcgtccatca acaccgccga
     5041 ttgggaatcg cggtgaaccc tgaaatagcg ccggaagctg gcgtcgccgg ccaggggccg
     5101 gatctcggcg ccgtcccagc catgctctgc aaggaaatcg ggcgcccctg cgggcggaat
     5161 catcggggcg gccatcgcgc tccccaagcg gcggggacgg cggctgtcaa ggcgcgggcg
     5221 ccctcccctt gccgccgcag ggtgaggcga agcgaatgcg gccataaggc gcggcccatc
     5281 cgctcgggcc attccacgat caaggccccg tcctggagcg cctcgtcgag cgccagctcc
     5341 tcgatctcgg ccggaccgtc gaggcgatag agatcgacat gccagaccgg aattcggacg
     5401 caatcatagg ggatgacgat cgggaaggtc gggctggcca cctcgccgtc atggccgagc
     5461 ccggcgagga tgccgcgcgc gagggtggtc ttgccggcgc ccagctcgcc gaacagggcg
     5521 acgacgtcgc cggcgcgaag atgcgccgcg agccgccggc cgatcgcctc ggtcgcctcg
     5581 ggcccgtcga ggaccatcag cgcttgcggg gcagtcgaag ggtgacggtg gtgccctcgc
     5641 caggctccga caccagctcg atcgtaccgc catgggcctc gatgaactgc ttggcgagcg
     5701 gaaggcccag gcccagcgct gccccgtcgg ccccgcgcgg ctccgcggtg cgcgagaacc
     5761 gatcgaagac tccggcctgg tccgccggag cgatcccccg gccattgtcc gaaaccgtga
     5821 tcgccgcttc cttgcggttg ccggcggcgt ggaggaagac ccgcccccct tcacccgtat
     5881 aggcgacggc attcttgagc acattgtcga gcgactggcg gagacgcgcc cggtctccgg
     5941 cgatcgagcc cgcgcccgct tcgatctcca cagcgaaatc gatctgcttc ttcgccgcct
     6001 gctccttgaa ggcccgggcc gcctcagcgc agagcctggc gaggtcgacc ttgtcctcgg
     6061 cgagcaggag gcttccgctg tcgctctggg tcaggtcgag cacgtcgtcg atcaaagcgc
     6121 ccagccgagc gactgaatcg aggatcgccg tcacatattc ggaagcggtc ggaccgaggt
     6181 ccccggcata gccgccggcg agcatctcgg caaagccgcc gatcgaggtc aagggcgtgc
     6241 gcagctcgta gctcatgttc gagacgaagg cggtcttcag ccggtcggcc tgctcgagcg
     6301 cctgggtacg ctccctcaga gccgcttcga ccgtcctgct ggcggtgacg tccagcatcg
     6361 tgaagagagc gttgccgtcg gggagcggaa cggcggcgaa ctcgaactcg cgcccgtcgg
     6421 tgaaggcgac ccggccgttg cgctgcttgc gctcgactgt ggcgctccgg acgagctcgc
     6481 ggaccaaagc cgcgtggctc gaattcctga gcttcttgcc gatatgggtg gtgagagcgt
     6541 cgatccgggg attgccggcc agttgctcct cgtcgaactc ccacaataag cggaagcggt
     6601 tgttccagag gtggaggcgt ccgtcggagg cgaagactcc caccgcctcg aacagattgt
     6661 cgaaggtcgc ggtccggacc cggagcaggg tatcgcgcgc cgaagccagg cgaagctgct
     6721 cggtccggtc ctcgaagatg agcagcaggc cgccgtcagg aagcggctgg gcgacgaccc
     6781 ggaggtggcg cccgccgggg agaagccaat cctcctcctc cgccgcgagg ccgccggtga
     6841 accagcggtc atgctccttc ttccattccg gatagtcgcg cacttccggc aggtgcccgg
     6901 tctcgcgcat cgcgtcgagc agccggttga actcgggccg gtcggcgagg aaatccggcc
     6961 tcagcgcgaa gaggcgcgcg aagggctggt tcgagaagtt gagcccgcga tcgcgggcga
     7021 actgggcaac tccggccgac aggcggtcga gcatgtcgcg ctgggcgtgg acgaagcggg
     7081 cgagctcgga gcgcgcctgc tcgcgatcct cgacgtcgat cgcgtagccg gcgaccccgg
     7141 cctcccccag cggcacttcg acgacccgca tcatccgccg ctccccgccg accgtcgcgg
     7201 gcacgttgcg ggcgtgcggc cggccgctct gccggaccgc ggcagcctcg gcgatcgctc
     7261 cggcctcgcc gtcgtcgacc agctccagcc cgccctcgat cacggctctg ccgtcctccg
     7321 cctcgacggc ggcgacatag gcgctgttga ccatggcgag gcgaagatcg ggcccgcgat
     7381 gccagatcgg gaaaggcgcg gcctcgatca gcccggacaa agcgtcgagc gccgaggcga
     7441 gcctttcgct gcgctcgcga agcgccgcca tctcctcctc gctctccgtc gcgtcggtga
     7501 gccagaggag gaccgtgccg gcgggatagg agggcggcgc cgggccgccg cggacccggt
     7561 agacccgggt ggagccggcg ggacggatcg agagcgccag gccggccgcg gtcgcggcgg
     7621 tcgcggcgac ctgcccgagc agggagtccc ggtcttcctc ggccagtgtg tcgccgagat
     7681 cgtcgagccg gcgcggcgtg gcggcgaggc cgagtgcaag cccgacccgc tccgcttcct
     7741 cgaggcttcc gtcgcggcgg acgatgagcg gaagcgccgg cgacgcggcg agcagggctt
     7801 cgtgatgcct ggtcgcgtcg attcgcgcgg tggcgtcgcg gccgcgcccg aggccgcgga
     7861 ccgtggccca gagggcgatc gcgagccacg ctccggccag gagcgcgatc gccgccaatg
     7921 cggtcggtga gaggtttacc atggagcggc ctctctaacc gcgctccgga gtctccggaa
     7981 gggcgtgcgt gagccgcggc aaaatgtgac attattgcca cgcagcggcg ggatatcact
     8041 cgataagccg ccgccaccat tggaatatcc gctctggccg gatagtccga ttacacaatt
     8101 aatcgtggag agggattcac cccatggcac tgacctttcg ctcgcgcgcc ctgcgctccg
     8161 tcgcttttat cgggctcctc ggcgcgctcg ctccccaggc gtacgcgcag gagactcagg
     8221 gcaccgaagg gaccggcgcc ggtgaaaccg acgatccctc ccagctgaac tcggaagccg
     8281 agatcgaatc cggccaggat gccagcgccg ccgatcaggg cgacatcacc atcaccgggt
     8341 cgc
//
LOCUS       DASWNU010000667         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_311184, whole genome shotgun sequence.
ACCESSION   DASWNU010000667 DASWNU010000000
VERSION     DASWNU010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311184"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VGB57_13415"
     CDS             <1..798
                     /locus_tag="VGB57_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEY0132393.1"
                     /translation="IERERVQMTGGVPTIAWQLLEHPERSRFDLSSLETISYGGAPAA
                     PELVRKIREEFGALPGNGWGMTETTATVTSHVGEDYLNRPDSAGPPVPISDLKIMSPD
                     GASELAIGEVGELWARGPQVVKGYWNRPEATAETFVDGWVRTGDLARLDEEGFVYIVD
                     RAKDMVIRGGENIYSIEVENVLFDHPAVTDAALIGLPHRTLGEEPAAVVHLAPGASAT
                     EEELKAWVRERLAVFKTPVRIAFLPQPLPRNANGKILKGELKALFDS"
     gene            complement(934..1887)
                     /locus_tag="VGB57_13420"
     CDS             complement(934..1887)
                     /locus_tag="VGB57_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY0132394.1"
                     /translation="MRLSPSVRRALLQHDIDPSRIEGTGRDGRITRADVDRAVAAATK
                     VQEGPRTTAQPSVIPAEAGTSGRPAEPVRPEVPASAGMTGVRSHSVPHDRMRLKIAEN
                     MLGSVTRAPHVTAMFEADFSAIVAHRERHKAAWAKKGVKLTYSAYIVAAAAEAMKVAP
                     AINSRWHEDRLEIFDDINIGVGTALGEKGLIVPVLRKVQTLSLKGIAAGLDELIEKAR
                     REQLTARDVSGGTFTISNHGVSGSLFAAPIIINQPQSAILGVGKLEKRVVVREVGGTD
                     AILIRPMAYVSLTIDHRVVDGHQTNAWLTRFVEVLEGWPAE"
BASE COUNT          306 a          651 c          678 g          304 t
ORIGIN      
        1 atcgagcggg agcgggtgca gatgacgggc ggagtgccga ccatcgcctg gcagttgctc
       61 gagcatcccg aacgctcgcg cttcgacctc tccagcctgg agacgatcag ctatggcggc
      121 gccccggccg cgcccgagct cgtccgcaag atccgcgagg agttcggggc gctccccggc
      181 aacggctggg ggatgacgga gacgacggcg acggtgacca gccatgtcgg cgaggattat
      241 ctcaaccgcc ccgacagcgc cggcccgccg gtcccgatct cggacctcaa gatcatgtcc
      301 cccgacgggg cgagcgagct ggcgatcggc gaggtcggcg aattgtgggc gcgcgggcct
      361 caggtcgtca aaggctattg gaacaggccg gaggcgaccg ccgaaacctt cgtcgacggc
      421 tgggtgcgta ccggcgatct cgcccgcctc gacgaggaag gcttcgtcta tatcgtcgac
      481 cgggccaagg acatggtcat ccgcggcggc gagaacatct attccataga ggtggagaac
      541 gtcctcttcg accacccggc ggtgacggac gccgccctga tcggcctccc ccaccgaacc
      601 ctcggcgagg agccggcggc ggtcgtgcac ctggcgccgg gggcgagcgc gacggaggag
      661 gagctcaagg cctgggtgcg cgagcggctg gcggtgttca agacaccggt gaggatcgcc
      721 ttcctgcccc agcccctgcc gaggaacgcg aatggcaaga tattgaaggg ggaattgaag
      781 gcgctgttcg actcctaaat cctccccggc ttttgccggg gagggggacc atgcgaagca
      841 tggtggaggg gttcttctcg aacgccgaag aacccctcca ccgcgcttcg cgcggtccgc
      901 ctcccccggg aatccggggg aggatttagg aagtcactcc gccggccacc cctccagcac
      961 ctcgacgaac cgcgtcagcc acgcattggt ctggtggccg tcaaccaccc tatgatcgat
     1021 cgtcagcgag acataggcca tcggccggat caggatcgcg tcggttccgc cgacttcgcg
     1081 aaccaccacc ctcttctcga gcttgccgac cccgaggatc gccgactgag gctggttgat
     1141 gatgatcggg gccgcgaaga gcgagccgga gactccgtgg ttggagatgg tgaaggtgcc
     1201 gcccgacacg tccctggccg tgagctgctc gcgcctcgcc ttttcgatca gctcgtccag
     1261 gcccgcggcg atgcccttca gcgacagggt ctgcaccttg cgaagcaccg ggacgatcag
     1321 ccccttttcg cccagggccg ttccgacgcc gatattgatg tcgtcgaaaa tctccagccg
     1381 gtcctcgtgc cagcggctgt tgatcgccgg cgcgaccttc atcgcctcgg ccgccgcggc
     1441 gacgatgtag gcgctgtagg tgagcttcac gccctttttc gcccaggccg ccttgtgccg
     1501 ctcgcgatgg gcgacgatcg cgctgaaatc cgcctcgaac atggcggtga cgtgcggcgc
     1561 ccgggtcacc gagcccagca tgttctcggc gatcttcagc cgcatccggt cgtggggcac
     1621 gctgtgcgag cgcacacccg tcatcccggc ggaagccggg acctcgggtc gaacaggctc
     1681 ggcgggtctc cccgaggtcc cggcttccgc cgggatgacg gagggctggg cggtggtgcg
     1741 gggaccctcc tggaccttcg tcgccgccgc cacggcgcgg tcgacgtcgg cgcgcgtgat
     1801 ccgaccgtcg cggccggtgc cctcgatccg ggaggggtcg atatcgtgct ggaggagggc
     1861 gcggcggacg gagggagaaa ggcgcatctc cgtcgcccca gcgaaggctg gggccccggg
     1921 gggtgaaggg ctccggcgc
//
LOCUS       DASWNU010000668         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_336637, whole genome shotgun sequence.
ACCESSION   DASWNU010000668 DASWNU010000000
VERSION     DASWNU010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336637"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VGB57_13425"
     CDS             <1..872
                     /locus_tag="VGB57_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132395.1"
                     /translation="APTKMVATATAALVGQGEFCMISLYNGPDAGIDVNGNSDINLGC
                     GMKSNCTGKECVTAGGASSITARPIAAVGGLDGDSNNFNKPTVLQPHSAAQKDPFAHL
                     PNPAPNVTKCQAGGILTESTVFDPNDHDPCFLSAKVGPSATLNVPDHVQSITIYGGDI
                     DFKGDVNATGNTWVMTGPNGQAGDLIIISQANLVMSSPLDGQYKGVLFYRDRRASNIE
                     IKINGGASSSLTGAMYFPTSDITYAGNSSMVVQCLQMVGQKLKFRGGATIQNNCTGTG
                     ASAFNQTVVRLIE"
     gene            869..1318
                     /locus_tag="VGB57_13430"
     CDS             869..1318
                     /locus_tag="VGB57_13430"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0132396.1"
                     /translation="MKMLTTLARDERGTSLIEFGFLIPFLALLTMGVVDLSRGLAERF
                     ALQQAVNQGLELVQARPAVADAGSSDVNYDFVKNHVVAVTGVAPAQVTMTRWLECDGT
                     DKGDVNGTCTGTQDSARYLRVRVTKDFVGEFYFDKIPMAATGTLRTQ"
     gene            1318..1956
                     /locus_tag="VGB57_13435"
     CDS             1318..1956
                     /locus_tag="VGB57_13435"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY0132397.1"
                     /translation="MLKRLLLRGENKGIGAVEFALIAPVLIAFVVGISQMGKLFFANA
                     DMKNAMAHGARAASVYPVPEDDATIKAVVNERLARTGGVAKAKVEIKRGVENGNNFLD
                     IEMTYQVPLDFIVYDMAPVTLKDTRRVYTQMAPRSTDGSTSTTSTSATSAGGTTSSTS
                     ATSASSTGGTTSATSASSTTSSTTSASTTSSSGNGNSNGNSSHGHGDCKKKC"
     gene            complement(1985..2119)
                     /locus_tag="VGB57_13440"
     CDS             complement(1985..2119)
                     /locus_tag="VGB57_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132398.1"
                     /translation="MGNTLHARLETKLDGKRWRRDARAARAVGRVACGRPAGRASPTP
                     "
     gene            2401..2787
                     /locus_tag="VGB57_13445"
     CDS             2401..2787
                     /locus_tag="VGB57_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HEY0132399.1"
                     /translation="MSDLTIAGLAKAGGVGVETVRYYQRRGLLAEPPRAGAVRRYGPA
                     DVRRLLFIRRAQAAGFTLEEIGELLALDSSHDRARVRALAGDRLAALDSKIAELEAAR
                     AALTRLAGACAAGTKGPCPILEAFEG"
     gene            complement(2827..3207)
                     /locus_tag="VGB57_13450"
     CDS             complement(2827..3207)
                     /locus_tag="VGB57_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132400.1"
                     /translation="MTIESRLTEADWADFRRARIADHVALGLPRDAAAAAAERAEARS
                     KARLEWIQGRFERMAELWALLGRYWADFAEAHPEIDLEEPDDRDLPLLIDPPEQAELE
                     AVEAEIAAARDRDVWPRHLYFDEV"
     gene            complement(3204..>3568)
                     /locus_tag="VGB57_13455"
     CDS             complement(3204..>3568)
                     /locus_tag="VGB57_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132401.1"
                     /translation="ASLCRAARARSGGTVGLETLAWERGVTGIEEEVVQGGKVVGKRL
                     KRSDAVFKMLLEGGDSGRYGPQIKALREKIEAELRPKIEAEVRAEIAEKQRIDGPKLR
                     KQLEEMLSDFNRRMGGNG"
BASE COUNT          615 a         1338 c         1088 g          527 t
ORIGIN      
        1 aggccccgac caagatggtc gcgacggcga ccgccgcgct cgtcgggcag ggcgaattct
       61 gcatgatctc gctctacaac ggaccggacg ccggaatcga cgtcaacggc aattccgaca
      121 tcaatctcgg ctgcggcatg aagagcaatt gcaccggcaa ggaatgcgtc acggccggcg
      181 gagcttcgtc gattaccgcg cgcccgatcg ccgcggtcgg cggcctcgac ggcgactcca
      241 acaatttcaa caagccgacc gtcctccagc cgcactccgc ggcccagaag gatccgttcg
      301 cccacctgcc gaacccggcg ccgaacgtga ccaaatgcca ggccggcggg atactcaccg
      361 agagcacggt cttcgacccg aacgaccatg acccctgctt cctctcggcc aaggtcggcc
      421 cgagcgcgac tctgaacgtt cccgaccacg tccagtcgat caccatctat ggcggcgaca
      481 tcgacttcaa gggcgacgtc aacgccacgg gcaacacctg ggttatgacc ggtccgaacg
      541 gccaggccgg cgacctcatc atcatttcgc aggccaatct ggtgatgagc tcgccgctcg
      601 acgggcagta taagggcgtg ctcttctacc gcgaccggcg agcctccaac atcgaaatca
      661 agataaacgg cggcgccagc tcgagcctca ccggcgcgat gtacttcccg acctccgaca
      721 tcacctacgc cggcaattcc tcgatggtcg tccagtgcct gcagatggtc ggccagaagc
      781 tgaagttccg cggcggcgcc accattcaga ataactgcac cggcaccggg gcctcggcct
      841 tcaaccagac ggtcgtgagg ttgatcgaat gaagatgctt accaccctcg cccgcgacga
      901 gcgcggcacc agcctcatcg agttcggctt cctgatcccg ttcctggcgc tactgaccat
      961 gggcgtggtc gatctcagcc ggggcctcgc cgaacggttc gccctccagc aggcggtcaa
     1021 ccagggcctc gagctcgtcc aggcccggcc ggcggtcgcc gacgcgggct ccagcgacgt
     1081 caattacgac ttcgtcaaga accatgtcgt cgccgtcacc ggcgtggcgc cggcccaggt
     1141 gacgatgacc cgctggctcg aatgcgacgg caccgacaaa ggcgacgtca acggcacctg
     1201 cacgggcacc caggactcgg cccgttacct cagggtccgg gtgaccaagg atttcgtcgg
     1261 cgaattctat ttcgacaaga tcccgatggc ggccaccgga acgctgagga cccaatgatg
     1321 ctgaaacggc ttcttcttcg tggcgagaac aagggtatcg gcgcggtcga gttcgctctg
     1381 atcgccccgg tgctgatcgc gttcgtggtc ggcatcagcc agatgggcaa attgttcttc
     1441 gccaatgcgg acatgaagaa cgccatggcc catggcgctc gcgccgcctc ggtgtatccg
     1501 gtgcccgagg atgacgccac gatcaaggcg gtggtcaacg aacggctcgc ccgcaccggc
     1561 ggagtcgcca aggccaaggt cgaaatcaag cgcggcgttg agaacggcaa caacttcctc
     1621 gatatcgaga tgacctacca ggttccgctc gacttcatcg tctacgacat ggctccagtc
     1681 acgctcaagg acacccgccg ggtctacacc cagatggcgc cgcgaagcac cgacggatcg
     1741 accagcacca cgtcgaccag cgccacctcg gcgggcggaa cgacctcgtc gaccagcgcc
     1801 accagcgcca gctcgaccgg cggcacgacc agcgcgacca gcgccagctc gacgacgagc
     1861 tcgacgacca gcgccagcac gaccagctcg tcgggcaacg gcaacagcaa cggcaacagc
     1921 agccacggcc acggcgactg caagaagaag tgctaggctg agccgccgcg aacagccgcg
     1981 ctgttcacgg agtcggcgaa gcccggccgg cgggtctgcc gcaggccacc cgtccgacgg
     2041 cgcgggccgc ccgcgcgtcc ctgcgccagc gcttcccgtc gagcttcgtc tccagcctcg
     2101 catggagggt gttgcccatc gccccggggt ctcctgcccg gccgccttgg cgtggcgccc
     2161 cggcggcgac tgatccgccg cttgactccg taccatggta cggcagctat gggacgattc
     2221 gcggccggcg catcgggttc cggccacgac aggagaacga ccatgaagac acgcaccaac
     2281 gcccgcggcg gctgccgctg caacccctgc gcctgcagga attgcggctg cagctgagcc
     2341 tcgattcccc tctcccgccc gggcgggaga ggggcccacc ctggcgaagg agcccccgca
     2401 atgtccgacc tcaccatcgc cgggctcgcc aaggcgggcg gagtcggagt cgagacggtc
     2461 cgctattacc agcgccgcgg ccttctcgcc gagccgccga gagccggagc cgtccggcgc
     2521 tacggcccgg ccgacgtccg ccggctgctc ttcatccgcc gggcccaggc ggccggcttc
     2581 accctcgagg agataggcga gctcctcgcc ctcgattcga gccacgaccg ggcccgggtc
     2641 cgcgccctcg ccggcgaccg cctcgccgcg ctcgattcga agatcgccga gctcgaagcg
     2701 gccagggccg ccctcacccg cctcgccgga gcctgcgccg ccggaacgaa ggggccgtgc
     2761 cccatattgg aggcgtttga aggctgaccg ccttccccga tcgaagcacc gcttcgatcg
     2821 gatccctcag acctcgtcga aatacagatg ccgcggccac acgtcgcggt cccgggccgc
     2881 cgcgatctcc gcctccaccg cctcgagctc cgcctgctcg ggcgggtcga tcaggagggg
     2941 caggtcgcga tcgtccggct cctcgaggtc gatctcggga tgcgcctcgg cgaaatcggc
     3001 ccagtagcgg ccgagcaagg cccacagctc ggccatccgc tcgaaccgcc cctggatcca
     3061 ttcgagccgg gccttgctcc gagcctcggc ccgctccgcc gccgccgcgg cggcgtctcg
     3121 cggaagcccg agcgcgacat gatcggcgat ccgggcgcgc cggaaatcgg cccagtcggc
     3181 ttccgtcaac cggctctcga tggtcatcca ttgcctccca tccgccggtt gaagtcggac
     3241 agcatctcct cgagctgctt gcgcagcttc ggaccgtcga tccgctgctt ctcggcgatc
     3301 tcggcccgga cctccgcttc gatcttcggc ctcagctcgg cctcgatctt ctcccgaagc
     3361 gccttgatct gaggcccgta gcggcccgaa tcgccgccct cgagcagcat cttgaacacc
     3421 gcgtcggagc gcttgagcct cttcccgacc accttgccgc cctgcacgac ctcctcctcg
     3481 atcccggtca ccccgcgctc ccaggccagg gtctcgaggc ccaccgtccc gcccgaccgc
     3541 gcccgcgccg cccggcacag ggacgcga
//
LOCUS       DASWNU010000669        10696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3422
            k141_337147, whole genome shotgun sequence.
ACCESSION   DASWNU010000669 DASWNU010000000
VERSION     DASWNU010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959241
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:50:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,703
            CDSs (total)                      :: 2,673
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 0 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337147"
                     /isolate="SMAG_U3422"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VGB57_13460"
     CDS             complement(<1..640)
                     /locus_tag="VGB57_13460"
                     /inference="COORDINATES: protein motif:HMM:NF025137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEY0132402.1"
                     /translation="MGNRQDNRSRGNAAQLLEKYKSLARDAQLQGDRVQSEYWLQFAE
                     HYFRVLNENRARFEEQRPRREDYGSDEDEDQDESMAADGDDGDEDEGDRHIRDDRPSR
                     DDRGNREDRGNRDDRGYRTERAERVERPERNDRPERNERPERNERPERAEQPERPRRE
                     FQPREERNGERRPEGLRPRRERPEPRVEAHNDSDERISLDVLPPAIAVIEGDA"
     gene            complement(979..1848)
                     /gene="prmC"
                     /locus_tag="VGB57_13465"
     CDS             complement(979..1848)
                     /gene="prmC"
                     /locus_tag="VGB57_13465"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY0132403.1"
                     /translation="MKGKEKPSPVNAGEGIRGALGAATSRLAAHSDTPRLDAEVLMAH
                     ALGMERDQLLLADKDRKPPPEFETLVSRREAGEPIAYIVGRRGFWTIELEVGPGVLVP
                     RPDSETLIEAAVAHFGAAAPQRILDLGTGPGTLLLAALDQWPQATGLGVDSSETALDY
                     ARRNAERLDLSARAAFRKGDWAEGIDRIFDLILCNPPYVESGADLPRDVAEWEPAEAL
                     FAAADGLSEYRRLAPQIPRLLAPGGLACIELGAGQRDAVAALFDGSGLSLSSRRDLDN
                     IERCLTLSREIGR"
     gene            complement(1838..2917)
                     /gene="prfA"
                     /locus_tag="VGB57_13470"
     CDS             complement(1838..2917)
                     /gene="prfA"
                     /locus_tag="VGB57_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0132404.1"
                     /translation="MTSISAGRIAAIEARREELQNAMSSPALAPDEFVKLSKDYAEIE
                     PVAAAAREVRRIRAEIELLAEMLADPSPEIREMASEELAGLQAQLPEAERALALKLLP
                     SDAADQRPAMLEIRAGTGGDEAALFAGDLFRMYQRYAENQGWKVELISASSADMGGYK
                     EVVASVRGQGVFARLKYESGVHRVQRVPVTESGGRIHTSAATVAVLPEAEEVDVQIDE
                     ARDLRIDVYRSSGPGGQSVNTTDSAVRITHLPTGLVVIQQDEKSQHKNKAKALKVLRT
                     RLYEQERERLASERAGARKSMVGSGDRSERIRTYNYPQGRVTDHRINLTLHRLPEILE
                     GQLDELIGALISEDEAERLSSLDEG"
     gene            complement(2914..4158)
                     /gene="hisS"
                     /locus_tag="VGB57_13475"
     CDS             complement(2914..4158)
                     /gene="hisS"
                     /locus_tag="VGB57_13475"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY0132405.1"
                     /translation="MSKGPQRVRGTQDIWGEDADRFRAVVAAFERVRRLYGFGRVEMP
                     VFESTAVFARSIGETTDIVSKEMYSFEDRGGDSLTLRPEFTAGICRAYLSEGWQQHAP
                     VKIATHGPAFRYERPQKGRYRQFHQLDAEIIGAGEPASDVELIAMGAQLLAELGIGER
                     VDLKLNTLGDPATRDAWRAALVDYFQDHRNDLSAESRARLEKNPLRILDSKEHQDWPI
                     VDASPVVDDFLTLEAADYFGKVTAGLDSAGIVWKRDPRLVRGLDYYRHTAFEFVTEDL
                     GAQSAVIAGGRYDGLIESLGGPHTPAVGWAAGIERLGMLIEQPARPGIDAALIPLGAA
                     AETAAIGLVAQLRRSGIACEMAYKGNMKKRMQKADSSGARFAIILGDDELARGEAAVK
                     DLTTGEQKSVPIAGLAEAVRSR"
     gene            4451..5992
                     /locus_tag="VGB57_13480"
     CDS             4451..5992
                     /locus_tag="VGB57_13480"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0132406.1"
                     /translation="MRRTAPLFAVALLASTAVAADRTPEFSSSESATANTKAALKRIK
                     ALNPRINAVIAVDPTALGQARTLDRIRRARGPLFGMPILIKDNIESLGPLPTTAGSLA
                     LAGNVTNRDAPLVARLRQAGAVIVGKANLSEWANIRDNDSISGWSAVGGQTRNPHALN
                     RNACGSSTGSGAAVAAGMVPAAIGTETDGSITCPAALSGIVGFKPTVGLVSRTHVVPI
                     SHSQDTPGPMTRTVRDAALVLSAIAGSDPADPQTREADSRRADYAAGLAADSLRGKRI
                     GVMRFATGFGTDAPFEAALQTLRSQGAVLVEIAKFERKPEMGAGEFKVLLTELKADLN
                     AYLATTPPAVRTRTLAEVIAFNKGNAREMALFDQDTFEEAEKTKGLDDPEYRKARETS
                     LRLAGPEGIDKMLKDNNVIALVGPTMPPAWPIDAVNGDQISGGGAGGFAAVAGYPHLT
                     VPMGHVKGLPVGLSFIGPKWSDGMILSLGYAYEQASRKRVEPRFLPSIEESPEVAPLM
                     RPVAR"
     gene            complement(6038..6424)
                     /locus_tag="VGB57_13485"
     CDS             complement(6038..6424)
                     /locus_tag="VGB57_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY0132407.1"
                     /translation="MSFDEGLYAWVQEALEPLGAVTMRKMMGGATLYLDGIVFAILDE
                     GEIWLKADAESDAVWDSQGCEKFSVTFKDGKVDTMNYRRAPQDDYDDPDAMRRWAALA
                     VEAGLRGAAKKRPRPSSRRKPGPQAD"
     gene            complement(6421..6738)
                     /locus_tag="VGB57_13490"
     CDS             complement(6421..6738)
                     /locus_tag="VGB57_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEY0132408.1"
                     /translation="MAWLLLIVGGLFEVGFTTCLRFVDGFRNLPWTLGFLASVTLSMG
                     LLERAARDIPMGTAYAIWGGIGAAGTVLVGILWFGEPVNSIRILLLLALIACIVGLRL
                     TAA"
     gene            complement(6781..7686)
                     /locus_tag="VGB57_13495"
     CDS             complement(6781..7686)
                     /locus_tag="VGB57_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0132409.1"
                     /translation="MSAPELHPRPIVPFAVATAGIALFSTMDALMKGLSLAIGTYNAL
                     LWRTLAGAAIGGAVYFLRRSPRPGRAAIRVHLIRGIMGSVMAMTFFWGLARVPMAQAI
                     ALAFVAPLIALYLAAVLLKERIERAAILASLLGFAGVVVILAGQAQAEMGREALLGSI
                     SILASAGLYGWNIILMRQQAQLAGPVEIAFFTALIMTLCFLLAAPFLATPPPPAQMPA
                     ILAAAVLAFGSLMLLSWAYARAEAQRLAPVEYTGFLWAAIFGWLIFAEPIQPLTLAGA
                     AMIVVACWVATRPSRTPMPAVEPGA"
     gene            complement(7766..8257)
                     /locus_tag="VGB57_13500"
     CDS             complement(7766..8257)
                     /locus_tag="VGB57_13500"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY0132410.1"
                     /translation="MQKAFLLMVSAGLVIGVAWPSDRPAAPVVAAMDSAAGAEGGEDE
                     PRETVLERSGGGHFYADVEVNGELVRFIVDTGASGVALTERDAKRVGLAFDKGEYEQV
                     GTGASGTVRGKFVTLDKVSLDGKAAHKIEGAILEGSEISLLGQNYLGRFSIEIRGNTM
                     RIY"
     gene            complement(8318..8959)
                     /locus_tag="VGB57_13505"
     CDS             complement(8318..8959)
                     /locus_tag="VGB57_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY0132411.1"
                     /translation="MSAHCCHEKGHELERIALHKDIRRVLVVVMMLNLMMFVLEFGAG
                     IIADSAALMADSVDMLGDGLVYGISLYALNRSLRWRAGMALAKAGFILALGLGVIVQI
                     VLKIVWGQPPESGIMLIFGGMALVVNLSCVALLWPYRRHDVNLSSTFECSRNDVFANI
                     GVLAAAVLVSVTASPWPDIAIAAVIAYLFFRSSLKVTRDAWPQYRHAHAHPAE"
     gene            complement(8956..9429)
                     /locus_tag="VGB57_13510"
     CDS             complement(8956..9429)
                     /locus_tag="VGB57_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132412.1"
                     /translation="MSGDRLALILAKQKQAYEQRTAASEEQRRRQLEKERRVQNLLET
                     WNGSQVTLRAVIAELNREMQANGVSLRYREETSLAGVRRSAEITYDRPQEYILTGLSL
                     RLYLDSQGLLTVTIVGMGRPIKNEKIKLIDFVEDVQRGWLLDFLEATAEAEAGER"
     gene            complement(9442..10572)
                     /gene="ispG"
                     /locus_tag="VGB57_13515"
                     /gene_synonym="gcpE"
     CDS             complement(9442..10572)
                     /gene="ispG"
                     /locus_tag="VGB57_13515"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0132413.1"
                     /translation="MSIRPWRDIARRQSRQIMVGSVPVGGDAPVTVQTMTNTVTADVR
                     ATVDQIRRCEEAGADIVRVSCPDEDSTRALRQIVRAARVPIVADIHFHYRRALEAADA
                     GAACLRINPGNIGSADRVREVVNAAKANGCAIRIGVNAGSLERDLLEKYGEPCPEALV
                     ESALDHIRLLEDHDFREYKVAVKASDVFLAVAAYQQLAEAVDCPLHLGITEAGGLIGG
                     TVKSSIGIGALLWFGIGDTIRVSLSAEPEEEVKVGFEILKSLGIRNRGVRVVSCPSCA
                     RQGFDVIRTVQALEGRLAHIRTPMSLSVLGCVVNGPGEARETDIGITGGGNGKHMVYL
                     SGVTDHHVRDEEMVDHIVRLVEAKAAEIERAMTDAPISDAAE"
BASE COUNT         1726 a         3632 c         3621 g         1717 t
ORIGIN      
        1 gcgcgtcccc ttcgatcacc gcgatcgccg gcggaagcac gtcgagcgag atcctctcgt
       61 cggaatcgtt atgcgcttcg acacgcggct ccgggcgttc gcgacgcggg cgcaggccct
      121 cggggcggcg ctcgccgttc cgctcctcgc gcggctggaa ctcgcggcgc ggccgttccg
      181 gctgctccgc gcgctccgga cgctcgttgc gctccggacg ctcgttgcgc tccggacgat
      241 cgtttcgctc cggacgctcg acgcgctccg cccgctccgt gcgataaccc cggtcatcgc
      301 ggttgccgcg gtcttcccga ttgccgcggt cgtcgcggct ggggcggtcg tcgcggatgt
      361 ggcgatcgcc ctcatcctcg tcgccgtcgt cgccgtcggc agccatcgac tcgtcctgat
      421 cctcatcctc gtcggatccg taatcctcgc gacgggggcg ctgctcctcg aagcgggcgc
      481 ggttctcgtt caagacccga aaataatgct cggcgaactg cagccaatat tcggactgca
      541 cgcgatcgcc ctggagctgg gcgtcgcgcg ccagcgactt gtatttctca aggagctggg
      601 cggcattgcc gcgcgagcgg ttgtcctgcc ggttgcccac attggacgac tggttcggcg
      661 aacgcgggcc gctgcccccg cggccgcgac ggcggccgtt ttgacgattg tttatcaaat
      721 gtaggtcctc gtcctcgtgg tcctattcac gccaccgtga caaccgggcc cctcccggcc
      781 gcaaacttaa gcacgacacc cgggcagggc cgcacatgcg gcctccgcca cctcacgctg
      841 cgaacgtcct gggacgtcta agcaatcggg ggtcggcgac ggttggcctc acgcattcat
      901 gctgtgaaga gccccgtccc tgaaccccta tgtagctatg cccgcaacga aatccaagcg
      961 gaaagatgat gacgtggatc agcgtccgat ctctcggctt agcgtcaggc aacgctcgat
     1021 attatcaagg tccctgcgtg aggacaggct taaccccgag ccgtcgaaca gcgccgccac
     1081 cgcatcgcgc tggccggcac cgagctcgat gcaggcgaga ccgccggggg cgagcaatcg
     1141 cgggatctgc ggcgcgagcc ggcggtattc ggagagtccg tcggccgcgg cgaacagggc
     1201 ttcggcgggc tcccattccg ccacgtcgcg aggcaggtcg gcgccgctct ccacataagg
     1261 gggattgcac aggatgaggt cgaagatccg gtcgatcccc tcggcccagt ctccctttcg
     1321 gaaggcggcc cgtgcggaca ggtcgaggcg ctcggcgttg cggcgtgcgt aatcgagcgc
     1381 cgtttccgat gaatcgacgc cgaggccggt cgcctgtggc cattggtcga gagccgcaag
     1441 cagcagggta cccggccccg tgccgagatc gagaatgcgt tgcggagcgg cggcgccgaa
     1501 atgcgccacc gccgcttcga tcaaggtctc gctgtcgggt cggggaacca ggacaccggg
     1561 cccgacttcg agctcgatcg tccaaaaccc ccgccgcccg acgatgtagg cgattggttc
     1621 gccggcttcg cggcgggaaa cgagagtttc gaattcaggc ggcggctttc gatccttgtc
     1681 ggcaagcaac agctggtccc gctccatccc cagagcgtgc gccatcagga cctcggcgtc
     1741 gaggcggggg gtgtcgctat gcgcggcaag acgggaagtc gcggcgccga gggcgcctct
     1801 gatcccttct cccgcattta cgggagaggg tttttcctta cccttcatcc aggctcgaca
     1861 gccgctcggc ctcgtcctcc gagatgagcg ctccgatcag ctcgtcgagc tggccttcga
     1921 gtatttcggg aaggcggtgc agggtgaggt tgatgcgatg gtcggtcacc cgcccctgcg
     1981 gataattgta ggtccggatc cgctccgagc ggtcgcccga gccgaccatc gacttgcgcg
     2041 ctccggcgcg ctcggaggcg aggcgctcgc gctcctgctc gtagaggcgg gttcggagca
     2101 ccttgagggc cttggccttg ttcttgtgct gcgacttctc gtcctgctgg atgacgacca
     2161 ggccggtggg aagatgggtg atccggaccg cgctgtcggt ggtgttgacc gattgtccgc
     2221 cgggccccga ggagcggtag acgtcgatgc gcagatccct ggcctcgtcg atctggacgt
     2281 cgacctcctc cgcctcgggc aggaccgcca ccgtcgccgc ggaggtatgg atccggccgc
     2341 cgctctccgt caccggaacg cgctggacgc ggtggacccc gctttcatat ttcagcctgg
     2401 cgaacacacc ctggccgcgg accgaggcga cgacttcctt gtagccgccc atgtcggcgg
     2461 agctggccga gatgagctcg accttccagc cctggttctc ggcgtagcgc tggtacatgc
     2521 ggaacaggtc gccggcgaac aaggcggctt cgtcgccgcc ggtgccggcg cggatctcga
     2581 gcatcgcggg gcgctggtcg gccgcgtcgc tgggcaggag cttgagcgcc agcgcccgtt
     2641 ccgcctcggg cagctgcgcc tgaagcccgg ccagctcctc ggaagccatc tcgcggatct
     2701 cggggctggg atcggccagc atctcggcga gaagctcgat ctcggccctt atccgccgga
     2761 cttcgcgagc ggcggccgcg accggctcga tctcggcata atccttggag agcttcacga
     2821 actcgtccgg cgccagggcc ggcgacgaca tcgcgttctg aagctcctcg cgccgcgcct
     2881 cgatcgcggc gatccgtccg gccgagatcg aggtcatcgc gaccgcactg cctcggccag
     2941 gccagctatc gggacgcttt tctgctcgcc cgtcgtcaga tccttcaccg ccgcctcgcc
     3001 ccgggcgagt tcgtcgtcgc cgaggatgat ggcgaagcgg gcgccgctcg aatccgcctt
     3061 ttgcatccgt ttcttcatat ttcccttata ggccatctcg caggcgatcc ccgagcgccg
     3121 cagctgggcg acgaggccga tggccgccgt ttccgccgcc gctccaagcg ggatcagagc
     3181 ggcgtcgata cccggcctgg ccggctgctc gatcagcatc ccgagccgtt cgatccccgc
     3241 cgcccagccg accgcgggcg tgtgcgggcc gccgaggctc tcgatgagcc cgtcatagcg
     3301 gccgccggcg atcaccgcgc tctgcgcccc gagatcctcg gtgacgaact cgaaggcggt
     3361 gtggcggtaa taatcgaggc cccggacgag gcgcgggtcg cgcttccaga cgattccggc
     3421 ggagtcgagg ccggcggtga ccttgccgaa atagtcggcg gcctcgaggg tcaggaaatc
     3481 gtcgaccacc ggcgacgcgt cgacgatcgg ccagtcctga tgctccttgc tgtcgaggat
     3541 gcggagcggg ttcttctcga gccgcgcgcg gctctcggcg gagaggtcgt tgcggtgatc
     3601 ctggaagtaa tcgaccaagg cggcccgcca ggcatcgcgg gtggcggggt cgcccagcgt
     3661 gttgagcttc agatcgacgc gctcgccaat gccgagctcg gcgagcagct gcgctcccat
     3721 ggcgatcagc tcgacgtcgg acgccggctc gccggcgccg atgatctcgg cgtcgagctg
     3781 gtggaactgg cggtagcggc ccttctgcgg ccgctcgtag cggaaggcgg ggccgtgggt
     3841 ggcgatcttg accggcgcgt gctgctgcca gccttcggag agataggctc ggcagatccc
     3901 ggcggtgaat tcggggcgga gggtgaggct gtcgccgccg cggtcctcga agctgtacat
     3961 ctccttggag acgatgtcgg tggtctcgcc gatcgaccgg gcgaagacgg ccgtgctctc
     4021 gaagaccggc atctcgaccc ggccgaagcc gtagagcctt cgaacccgct cgaacgccgc
     4081 gaccaccgcc cggaagcgat cggcgtcctc gccccagatg tcctgggtgc cccttactct
     4141 ctgcggtccc ttgctcaccg gcgcggcctt agcatttttg gaggcggggg gaagggatcg
     4201 ggtttccccc tctcccccgc ggggagaggg ttggggtgag ggggctccag gttcgagact
     4261 ccctttcgaa ggccgaaccc cctcagccta ccctctcccg acaggggaga ggggctttcg
     4321 gcgcatgccc ctcctgcacc agatcgccca tcccgacatc gcccattctt tccggaaagg
     4381 cggcggtgga ggctggacgg acgatgcggg aggcgccatc gcgttgtaaa agctcaggag
     4441 agtcgcacac atgcgccgta ccgccccttt gttcgccgtc gcccttctcg cgtcgaccgc
     4501 cgtcgcggcc gaccggacgc cggaattcag ctcgtcggaa tccgccaccg ccaacacgaa
     4561 agcggctctg aagcggatca aggcgctcaa tccgaggatc aacgccgtca tcgcggtcga
     4621 tccgaccgcc ctcggccagg cccggaccct cgaccggatc cggcgcgccc gggggccgct
     4681 gttcgggatg ccgatcctga tcaaggacaa tatcgagagc ctgggcccgc ttccgaccac
     4741 ggcggggagc ctggcgctgg ccggcaacgt caccaaccgc gacgcgcccc tggtggcgag
     4801 gcttcgtcag gccggcgccg tcatcgtcgg caaggccaat ctgtccgaat gggccaacat
     4861 ccgcgacaac gacagcatct ccggctggag cgcggtcggc ggccagaccc gcaacccgca
     4921 tgcgctcaac cgcaacgcct gcggatcgag caccgggagc ggcgccgcgg tggcggccgg
     4981 aatggtgccg gcggcgatcg gcaccgagac cgacggctcg atcacctgcc cggccgcgct
     5041 cagcggaatc gtcggcttca agcccacggt cggcctggtc agccggaccc atgtcgtccc
     5101 gatcagccac agccaggaca cgcccgggcc gatgacgcgg acggtccgcg acgccgcgct
     5161 cgtcctctcc gccatcgccg gaagcgaccc cgccgacccg cagacccgcg aggccgattc
     5221 gcgccgggcc gactatgccg cgggcctcgc ggccgattcg ctgcgcggca aaaggatcgg
     5281 cgtgatgcgc ttcgcaaccg gcttcggcac cgacgccccg ttcgaggcgg cgcttcagac
     5341 cctgaggagc cagggcgcgg tcctcgtcga gatcgccaag ttcgagcgca agcccgaaat
     5401 gggcgcgggc gagttcaagg tgctgctcac ggagctcaag gccgatctca acgcctatct
     5461 cgccacgacc ccgcccgccg tcaggacccg cacccttgcc gaggtcatcg ccttcaacaa
     5521 gggaaatgcc cgagagatgg cgctgttcga ccaggacacg ttcgaggagg cggagaagac
     5581 caaaggcctc gacgatcccg aatacaggaa ggcgcgggag accagcctta ggctcgccgg
     5641 gcccgagggg atcgacaaga tgctgaagga caataacgtc atcgccctcg taggaccgac
     5701 catgccgccg gcctggccga tcgacgcggt caacggcgac cagatttcgg ggggaggcgc
     5761 cgggggattc gccgcggtcg cgggctatcc gcacctcacc gtgccgatgg gccatgtgaa
     5821 ggggctgccg gtgggcttga gcttcatcgg cccgaaatgg tcggacggga tgatcctctc
     5881 cctgggctat gcctatgagc aggcttcgag gaagcgggtg gagccgcgct tcctgccgtc
     5941 gatcgaggag agcccggagg tggcgccgtt gatgcggccg gtagcgcggt aaccgtcatc
     6001 ccggcgaaag ccgggagctc ggggagaccc gcggtgccta atcggcctga ggtcccggct
     6061 ttcgccggga tgacggtctg gggcgcttct tcgccgcgcc gcgcagcccc gcttccacgg
     6121 ccagcgccgc ccagcggcgc atcgcgtccg ggtcgtcgta gtcgtcctgc ggcgcgcggc
     6181 ggtagttcat ggtgtcgacc ttgccgtcct tgaacgtgac gctgaacttc tcgcaccctt
     6241 gcgagtccca aacggcatcg ctctccgcat ccgccttcag ccagatctcg ccctcgtcca
     6301 ggatcgcgaa gacgatgccg tcgagataga gcgtcgcgcc gcccatcatc ttcctcatcg
     6361 tcaccgcgcc caagggctcg agcgcctcct gcacccaggc gtagaggccc tcgtcgaagc
     6421 tcatgccgcc gtcagccgca gcccgacgat gcaggcgatc agcgcgagca ggagcagtat
     6481 ccggatcgaa ttgaccggct cgccgaacca gaggatcccg accagcaccg tcccggccgc
     6541 tccgatcccg ccccagatgg cgtaggcggt gcccatgggt atgtcgcggg cggcgcgttc
     6601 gagaaggccc atggacaggg tcaccgaggc gaggaagcca agcgtccagg gcaggttcct
     6661 gaagccgtcg acgaagcgaa ggcaggtggt gaagccgacc tcgaagaggc cgccgacgat
     6721 gaggagaagc caggccatgg tgactgtcac tagtgactgt cactagtgac tgtcactccc
     6781 tcacgcgccc ggctcgaccg ccggcatcgg cgtccggctc gggcgagtcg cgacccagca
     6841 ggcgacgacg atcatcgccg cgccggccag ggtcagcggc tggatcggct cggcgaagat
     6901 cagccagccg aagatcgccg cccagaggaa gccggtatat tcgaccggcg ccagccgctg
     6961 agcctccgcc cgggcgtagg cccaggagag gagcatgagc gacccgaagg cgagcacggc
     7021 agcggccagg atcgcgggca tctgggccgg cggcggcggg gtcgcgagga agggcgcggc
     7081 gaggaggaag cacaaggtca tgatcagggc ggtgaagaag gcgatctcca ccggcccggc
     7141 gagctgcgcc tgctgccgca tcaggatgat gttccagccg taaaggccag ccgaggcgag
     7201 gatcgagatc gagccgagca gggcctcccg ccccatctcg gcctgcgcct ggccggcgag
     7261 gatcacgacg accccggcga agccgagcag cgaggcgagg atcgcggcgc gctcgatccg
     7321 ctccttgagc agcaccgcgg caaggtagag cgcgatcaag ggggcgacga aggcgagagc
     7381 gatcgcctgg gccatcggca cccgcgccag cccccagaag aaggtcatcg ccatgacgct
     7441 gcccatgatc ccgcggatca ggtgaacccg aatcgcggcc cggcccggcc gcggcgaccg
     7501 gcgcaggaag tagacggcgc cgccgatcgc ggccccggcc agggtccgcc agagcaaagc
     7561 gttgtaggtg ccgatggcga gggacaggcc cttcatcagc gcgtccatgg tcgagaagag
     7621 cgcgattccg gccgtcgcca ccgcgaaggg gacgatgggc cggggatgca attcgggcgc
     7681 gctcatggcc ccgccgatag cggcggcggc gaccgccgtc acgcttcgag gccccctaaa
     7741 ttcctccccc ggaaaaccgg aggatttagt agatgcgcat cgtgttgccg cggatctcga
     7801 tcgagaagcg gccgaggtaa ttctggccga gaagcgagat ctcgcttcct tccagtatcg
     7861 cgccctcgat cttgtgggcc gccttgccgt cgaggctgac cttgtcgagg gtcacgaact
     7921 tgccccgaac cgtccccgac gcgccggttc cgacctgctc atattcgcct ttgtcgaagg
     7981 cgagcccgac ccgcttggcg tcgcgctcgg taagagcgac ccccgaggct ccggtgtcca
     8041 cgatgaagcg gacgagctcc ccgttcactt ccacgtccgc gtagaaatgg ccgccgccgc
     8101 tccgctcgag caccgtctcg cgcggctcgt cctctccgcc ttcggcgccg gcggcggagt
     8161 ccattgcggc gacgacgggc gccgccggcc ggtccgaggg ccaggcgacg ccgatgacga
     8221 gaccggcgga gaccatgaga aggaaggctt tctgcatcgc gcgaagctag cgcgagacgg
     8281 tgaaggaaga cctaaaagcc gttccgggac gcggcgttta ttccgccgga tgggcgtggg
     8341 cgtggcggta ttgcggccag gcgtcgcggg tcaccttcag cgaggagcgg aagaagaggt
     8401 aggcgatgac cgccgcgatg gcgatgtccg gccagggcga ggcggtgacc gagaccagga
     8461 ccgccgccgc cagaacgccg atattggcga agacgtcgtt gcgcgagcat tcgaacgtgc
     8521 tcgacaggtt cacgtcgtgg cgccggtagg gccagagcag tgcgacgcag ctcaggttca
     8581 cgaccagggc catgccgccg aagatcagca tgatgccgga ctcgggcggc tggccccaga
     8641 cgatcttgag gacgatctgg acgatgaccc ccagcccgag ggcgaggatg aagccggcct
     8701 tggccagggc cattccggcc ctccacctga ggctccggtt gagcgcgtag aggctgatcc
     8761 cgtagacgag cccgtcgccg agcatgtcca ccgaatcggc catcagcgcc gccgaatcgg
     8821 cgatgatgcc ggcgccgaac tcgagcacga acatcatcag gttgagcatc atcaccacga
     8881 cgagcacccg gcggatgtcc ttgtggaggg cgatccgctc gagctcatgg cccttttcgt
     8941 ggcagcaatg ggcgctcatc tctctccggc ctccgcctct gcggtggctt ccaggaaatc
     9001 cagaagccag ccccgctgaa cgtcctcgac gaaatcaatg agctttatct tctcgttctt
     9061 gatcggccgg cccatgccga cgatggtgac ggtcaacagg ccttgcgagt ccaggtaaag
     9121 acgcagcgac agccccgtca ggatatattc ctggggccgg tcgtaggtga tttccgccga
     9181 acggcgaact ccggctaggg acgtctcctc ccgatagcgc agcgacacgc cattggcctg
     9241 catctcccgg ttcagctccg cgatcaccgc ccgcaaggtg acctggctgc cattccacgt
     9301 ctcgagaagg ttttgcacgc gtctttcctt ctccagctgc cggcggcgct gctcttccga
     9361 agcggccgtc cgctgttcgt aggcctgctt ctgcttcgcc aggatgaggg caagcctgtc
     9421 tccgctcatt tcacttcccg attattccgc ggcgtccgag atcggagcgt cggtcatcgc
     9481 cctttcgatc tcggcggcct tggcctcgac cagccggacg atgtgatcga ccatctcctc
     9541 gtcgcggaca tgatgatcgg tgaccccgga gagatagacc atgtgcttgc cgttgccgcc
     9601 gccggtgatg ccgatgtcgg tctcgcgcgc ctcgccgggg ccattgacga cgcagccgag
     9661 gacggaaagg gacatggggg tgcgtatatg cgcgagccgc ccttccaagg cctgaacggt
     9721 gcggatgacg tcgaaaccct ggcgcgcgca gctcgggcag gagacgaccc ggactccgcg
     9781 gttgcggatg ccgaggctct tcaggatctc gaagccgacc ttgacctcct cctcgggctc
     9841 ggccgagagc gagacccgga tcgtgtcgcc gatcccgaac cagagcaagg cgccgattcc
     9901 gatcgaggat ttgacggtcc ctccgatcag cccgcccgcc tcggtgatcc cgagatgcag
     9961 cgggcagtcg acggcttcgg cgagctgctg ataggcggca acggccagga acacgtcgga
    10021 ggctttgacg gcgaccttat attcgcggaa atcatgatcc tcgagcagcc ggatatgatc
    10081 caatgcgctc tcgaccagcg cctcgggaca gggctcgcca tatttttcga gcaggtcgcg
    10141 ctccaggctg ccggcattga ctccgatccg gatcgcgcag ccattggcct tggccgcgtt
    10201 cacgacctcg cgcacccggt cggccgagcc gatattgccc gggtttatgc ggaggcaggc
    10261 ggcgccggcg tcggcggcct cgagggcgcg cctgtagtgg aagtggatgt cggcgacgat
    10321 cggcacccga gccgcccgga cgatctgcct gagagcgcgg gttgaatcct cgtccgggca
    10381 ggagactcgg acgatgtccg ccccggcctc ctcgcagcgc ctgatctggt cgaccgtcgc
    10441 ccggacgtcg gcggtgacgg tgttggtcat ggtctgcacc gtgaccggcg catcgccgcc
    10501 gaccggcact gagccgacca tgatctggcg ggactgacgg cgcgcgatgt cgcgccacgg
    10561 gcggatgctc atcctaccca tataggcggt tgaaaggtct ggcgaaagcc gcaccgaaat
    10621 ggaggtaatg cggggagaga gcggagatgg cgtgcagcac ccatcctccc catgcgcagc
    10681 atggggaggg ggacca
//
